T100 Thermal Cycler Quick Guide

T100 Thermal Cycler  Quick Guide
AMPLIFICATION
T100™ Thermal Cycler
Quick Guide
Starting the Instrument
Remove the T100 thermal cycler from the box and
plug it in. Turn on the power switch on the back of
the instrument. The T100 cycler will perform a quick
self-test and then display the Home screen (Figure 1).
Setting the Date and Time
First make sure the system clock is correct for your
time zone so that all runs are accurately recorded
in the run log.
1. In the Home screen, touch Tools and then
Settings to display the instrument settings
menu (Figure 2).
Fig. 1. T100 thermal cycler Home screen.
2. T
ouch the fields for Month, Day, Year, Hours,
and Minutes and edit the values using the
pop-up keypad.
3. Touch Save to save the changes.
4.Touch Home to return to the Home screen.
Creating a New Protocol
1. To create a new protocol, touch New Protocol in
the Home screen.
2. T
he New Protocol screen displays a graphical
view of the protocol (Figure 3). Edit the value of
any of the following parameters by touching the
corresponding field:
■■
■■
■■
■■
■■
■■
Fig. 2. Settings screen.
Temperature
Hold time
Number of repeats in the GOTO loop
Starting step for the GOTO loop
Sample volume
Lid temperature
3. To select a step, touch anywhere outside the
temperature box. The step becomes highlighted
to indicate that it is selected. Once a step
is selected, you can insert a new step after
the selected step, delete the selected step,
or configure the options for the step.
Fig. 3. New Protocol screen showing graphical view
of a protocol.
4. T
o insert a step, touch Insert. The screen will display the
type of steps to insert (Figure 4). Select Temperature for a
temperature hold step, Gradient for a thermal gradient for
protocol optimization, or GOTO to insert a GOTO step that
will loop back to repeat the specified steps in your protocol
(typically denaturation, annealing, and extension).
5. T
o delete a step, select the step and touch Delete.
6. To configure advanced parameters for a step, such as
Temperature, Increment, Time, Extend, Gradient, and Ramp
rate, select a step and touch Options (Figure 5). Program
the required parameters as needed.
7.Touch Save to select a folder location and save the protocol.
8. Touch Run to start the protocol. You can also run a protocol
without saving it by touching Run at any time while editing.
Fig. 4. Insert step window.
Running and Editing a Saved Protocol
1. In the Home screen, touch Saved Protocols. The T100 cycler
organizes all the protocols into folders. A preinstalled library
of standard protocols is in the MAIN folder (Figure 6).
2. S
elect a folder from the Folders column, then select a protocol
file from the Files column.
3. Review the selected protocol using the Preview column
on the right.
4. Touch Run to start the run or touch Edit to edit the protocol.
For more information, visit
www.bio-rad.com/web/T100QuickGuide.
bio-rad.com/web/T100QuickGuide
Fig. 5. Options window showing advanced parameters.
http://www.bio-rad.com/en-us/product/t100-therm...
http://kaywa.me/tf2GM
This product is covered by one or more of the following U.S. patents or their
foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512
and 7,074,367.
Download the Kaywa QR Code Reader (App Store &Android Market) and scan your code!
Fig. 6. Saved Protocols screen showing the preinstalled
library of standard protocols in the MAIN folder.
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 3065 7550
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666
New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 026 674 55 05
Taiwan 886 2 2578 7189 Thailand 1800 88 22 88 United Kingdom 020 8328 2000
Life Science
Group
10020524 Rev B
US/EG
13-2043
1213
Sig 1213
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