PROTEAN i12 IEF System Quick Guide

PROTEAN i12 IEF System Quick Guide
ELECTROPHORESIS
PROTEAN i12™ IEF System
Quick Guide
®
18002 70994625354
1
Consult the PROTEAN i12 IEF System
Instruction Manual to ensure that all
components are present and for more
detailed instructions.
3
Choose rehydration method and rehydrate
strip. IPG strips can be rehydrated in the rehydration
tray or in the focusing tray. Consult the instruction
manual or 2-D Guide (bulletin 2651) for guidelines
regarding the choice of IPG strips, rehydration method,
IPG strip orientation, and loading method.
IPG Strips
Rehydration Tray
Prepare rehydration solution and sample.
1
2
3
4
5
1
6
4
9
3
8
2
7
5
6
7
12
11
10
8
01
-3
9
12
11
10
LN
2
1
2
3
4
5
6
7
8
9
12
11
10
Focusing Tray
To program an unattended run start following rehydration,
see step 6B
4
Choose focusing setup
A. Gel side down
2 Positive Electrode Assembly
1
2
3
4
5
6
7
8
12
11
10
9
3-1
5 IPG strip retainers
(one retainer should be placed
at each end of the focusing
tray after the tray has been
inserted into the instrument)
0N
L
4 IPG Strip
3 Wicks (recommended)
1
2
3
4
5
6
7
8
9
10
12
11
(press down)
1
2
3
1 Focusing Tray
4
5
6
7
8
11
10
9
12
2 Negative Electrode Assembly
B1. Gel side up
B2. Gel side up for sample loading with cups
4 Positive Electrode Assembly
1
2
3
4
5
6
7
8
12
11
10
9
5 Cup Holder
LN
01
-3
3 Wicks (recommended)
2 IPG Strip
Insert sample cup Properly inserted
at an angle
sample cup
or
1 Focusing Tray
2 IPG Strip
1
2
1
2
3
4
5
4 Positive and Negative
Electrode Assembly
6
7
12
11
9
8
9
LN
01
12
11
10
-3
12
11
10
10
8
9
7
8
6
7
5
6
4
5
3
4
2
3
1
3 Wick (recommended)
1
2
(press down)
3
4
5
6
7
8
9
12
11
10
1
2
3
4
1 Focusing Tray
5
6
7
8
10
9
12
11
4 Negative Electrode Assembly
To start run go to step 6; to program a method go to step 8.
Insert focusing tray assembled with IPG strip(s) into the PROTEAN i12 IEF system
Positioning Guide
Tray
Positioning Stop
Positioning the tray
1
2
3
4
5
1
6
2
7
4
5
6
7
12
11
10
9
3
8
8
-3
01
12
11
10
9
LN
5
Metal Grounding Strip
1
2
3
4
5
6
7
8
12
11
10
9
Positioning Guide
Tray
Positioning Stop
Location of
Common Negative Pin
Positioning
Guide
Instrument
Platform
To start run go to step 6; to program a method go to step 8.
© 2011 Bio-Rad Laboratories, Inc.
10022548
6
Programming — running a protocol
Choose a lane by highlighting the
desired field, then select protocol to
assign to the lane(s). When all lanes have
been populated as desired, press Run.
Note: View Protocol will only display the
protocol for the highlighted file on the right.
Choose Run.
6A. Focusing after rehydration is complete
6B. Programming rehydration into the run
Choose No for Rehydration. Press Strip
Details and Sample Details to add
information. Then press Start.
Choose Yes for Rehydration and set the
time in hours and minutes. Set the rehydration
for 0 volts or between 50–100 volts. Set
the temperature. Determine if you would
like the run to pause after rehydration.
Press Strip Details and Sample Details
to add information.
Use a pause if you want to add
wicks before focusing. Select No for
Pause after rehydration if you want
focusing to start automatically.
Press Start.
7
Run in progress
Rehydration in progress
Run in progress
End of run. Choose Export Results.
Highlight lane of interest and then click on View Protocol, View Data, or View Graph.
View Protocol
© 2011 Bio-Rad Laboratories, Inc.
View Data
View Graph
10022548
8
Programming – creating and editing protocols
Choose Edit to make changes to an
existing protocol.
Choose Create to create a new protocol.
Add and remove steps. Set desired voltage,
gradient, current, and time in minutes or
volthours by clicking in each box.
Choose Save and select file locations to
save protocol.
Press OK.
9
Saving data
After the run has ended, choose
Export Results.
10
Choose the location where the results
should be saved. To export from the
instrument, save to Flash Drive.
Exporting data
Remove Flash drive from instrument and
connect to PC.
Data can be opened in Excel in
comma-delimited format.
Go to www.i12Reporter.com to
upload data to an online graphing
and reporting application.
Excel is a trademark of Microsoft Corp.
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Malaysia 60 3 2117 5260 Mexico 52 555 488 7670
The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700
Russia 7 495 721 14 04 Singapore 65 6415 3170 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700
Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 Thailand 66 2 6518311 United Kingdom 020 8328 2000
Life Science
Group
10022548 Rev A
US/EG
11-0806
0811
Sig 0211
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement