Citizen GSX-190 User manual

Citizen GSX-190 User manual
C
COULTER® MD II™ Series Analyzer
Service Manual
7
4
1
_
8
5
2
0
9
6
3
.
TM
â
MD II
PN 4237242C (January 1998)
COULTER CORPORATION
Miami, Florida 33196
®
LEGAL NOTICES
Coulter Corporation makes no representation that, upon furnishing this service manual, the holder of the manual will have
the necessary technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the
manual. Coulter Corporation assumes no liability whatsoever, including consequential and incidental damages, resulting
from improper operation of Coulter instruments after maintenance of Coulter instruments has been performed by persons
not employed by Coulter Corporation. Furthermore, Coulter Corporation assumes no liability whatsoever for any personal
injury or property damage resulting from maintenance and/or repair of Coulter instruments performed by persons not
employed by Coulter Corporation.
READ ALL PRODUCT MANUALS AND CONSULT WITH COULTER-TRAINED PERSONNEL
BEFORE ATTEMPTING TO OPERATE INSTRUMENT.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING
CAUTION
IMPORTANT -
Might cause injury.
Might cause damage to the instrument.
Might cause misleading results.
Coulter Corporation urges its customers to comply with all national health and safety standards such as the use of barrier
protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when
operating or maintaining this or any other automated laboratory analyzer.
"This Service Manual contains confidential information of Coulter Corporation and its receipt or possession does not
convey any rights to reproduce, disclose its contents, or to manufacture, use, or sell anything it may describe.
Reproduction, disclosure, or use without specific written authorization of Coulter Corporation is strictly forbidden."
Copyright © Coulter Corporation 1995, 1996, 1998
All rights reserved.
C
REVISION STATUS
Initial Issue A, 8/95
Software Version 1.2
Revision B, 3/96
Software Version 1.3
Updated text for software level 1.3, updated the parts list, added specifications and procedures for the
optional Epson® TM-290P Slip Printer, added information about the ISL option, and made minor
corrections. The following pages were changed or added: vi, vii-xv, 2-ii, 2-iii, 2.13, 2.2-2, 2.4-5, 2.4-9,
2.4-12, 2.4-13, 3-i, 3.1-2, 3.2-1, 3.2-3, 3.4-1 through 3.4-4, 4.28-1, 4.28-2, 5.1-1, 5.2-1, 7.4-1, 8-i, 8.1-8
through 8.1-7, 8.2-1, 8.2-2, 8.2-4, 8.2-5, 8.2-8, 8.2-9, 8.2-12, A-i, A.1-1, A.2-1 through A.2-4, A.5-1, D-i,
D.1-1, D.1-2, E-i, E.1-1 and INDEX-1 through INDEX-11.
Revision C, 1/98
Released by CN 032060-0248
Software Version 1.3
Reformatted the manual to enhance the online version. Reformatting the manual changed the appearance
of each page, but it did not change the content. Consequently, a revision B manual and a revision C
manual look different but contain the same material.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, the changes will be summarized on a Notice of Information Update form and will be released
by service memo.
PN 4237242C
i
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, the changes will be summarized on a Notice of Information Update form and will be released
by service memo.
PN 4237242C
ii
C
CONTENTS
LEGAL NOTICES
REVISION STATUS, i
CONTENTS, iii
1
2
PN 4237242C
INTRODUCTION, 1.1-1
1.1
MANUAL DESCRIPTION, 1.1-1
Scope, 1.1-1
Organization, 1.1-1
Numbering Format, 1.1-1
Special Headings, 1.1-1
WARNING, 1.1-1
CAUTION, 1.1-2
IMPORTANT, 1.1-2
ATTENTION, 1.1-2
Note, 1.1-2
Conventions, 1.1-2
1.2
SAFETY PRECAUTIONS, 1.2-1
Electronic, 1.2-1
Biological, 1.2-1
Troubleshooting, 1.2-2
INSTRUMENT DESCRIPTION, 2.1-1
2.1
SYSTEM OVERVIEW, 2.1-1
Description, 2.1-1
Software Menu System, 2.1-2
Software Tables, 2.1-2
Aspirate Table, 2.1-5
Power-up Table, 2.1-6
2.2
POWER SUPPLY, 2.2-1
Overview, 2.2-1
AT Power Supply, 2.2-1
Inputs, 2.2-1
Outputs, 2.2-1
Switches, 2.2-2
AC Power/Vacuum Relay Card, 2.2-2
Inputs, 2.2-5
Outputs, 2.2-5
Test Points, 2.2-5
Jumpers, 2.2-5
+24 Volt Switching Power Supply, 2.2-6
Inputs, 2.2-6
Outputs, 2.2-6
Adjustments, 2.2-6
Linear Power Supply Card, 2.2-6
Inputs, 2.2-8
Outputs, 2.2-8
iii
CONTENTS
Test Points, 2.2-8
Jumpers, 2.2-8
iv
2.3
FLUIDICS PANEL, 2.3-1
Overview, 2.3-1
Diluter Panel, 2.3-2
Probe/Wipe Traverse Assembly, 2.3-4
Peristaltic Pump Assembly, 2.3-5
Syringe Assembly, 2.3-6
Individual Components Mounted to Fluidics Panel, 2.3-7
Cycle Counter and Air Solenoids, 2.3-7
Diluent Reservoir, 2.3-7
Flex Connect Card, 2.3-7
Lyse Pump (PM5), 2.3-8
Solenoid Interconnect Card, 2.3-8
Vacuum Regulator, 2.3-9
2.4
SYSTEM CONTROL, 2.4-1
AT Motherboard, 2.4-1
Switches and Jumpers, 2.4-1
User Resource Adapter (URA) Card, 2.4-2
Keypad Controller, 2.4-3
Display Controller, 2.4-3
CMOS RAM, 2.4-3
Diagnostic Data Acquisition Circuit, 2.4-4
Utility Timer Circuit, 2.4-4
Support Circuits, 2.4-4
Inputs, 2.4-5
Outputs, 2.4-5
Adjustments, 2.4-5
Switches and Jumpers, 2.4-6
Diluter Resource Adapter (DRA) Card, 2.4-6
Motor Controller, 2.4-6
Solenoid Controller, 2.4-7
Support Circuitry, 2.4-7
Inputs, 2.4-7
Outputs, 2.4-7
Jumpers, 2.4-8
Motor/Solenoid Driver Card, 2.4-8
Motor Driver, 2.4-9
Solenoid Driver, 2.4-9
Sensor Control, 2.4-9
+24 V POWER ON Signal, 2.4-9
+24 V POWERFAIL (PF/PG) Signal, 2.4-10
Inputs, 2.4-10
Outputs, 2.4-10
Test Points, 2.4-11
Jumpers, 2.4-11
PN 4237242C
CONTENTS
3
2.5
DATA ACQUISITION, 2.5-1
Sensor Preamp Adapter (SPA) Card, 2.5-1
Inputs, 2.5-1
Outputs, 2.5-2
Adjustments, 2.5-2
Jumpers, 2.5-2
Hgb Preamp Card, 2.5-3
Inputs, 2.5-4
Outputs, 2.5-4
Adjustments, 2.5-4
Test Points, 2.5-4
Vacuum Sensor Card, 2.5-4
Inputs, 2.5-5
Outputs, 2.5-5
Adjustments, 2.5-5
Test Points, 2.5-6
Sensor Processing Adapter with Diagnostics (SPAD) Card, 2.5-6
Power Supply Conditioning, 2.5-7
Oscillator Circuit, 2.5-7
Control Functions, 2.5-7
Octal Digital-to-Analog Converter (DAC), 2.5-8
Aperture Signal Processing, 2.5-8
Data Acquisition Circuit, 2.5-8
Test Pulse Generator, 2.5-9
Interrupt Selector, 2.5-9
Input, 2.5-9
Outputs, 2.5-9
Jumper, 2.5-10
Adjustments, 2.5-10
2.6
PERIPHERALS, 2.6-1
Floppy Disk Drive, 2.6-1
Display, 2.6-1
Keypad, 2.6-1
Rear Panel Interface Connectors, 2.6-1
INSTALLATION PROCEDURES, 3.1-1
PART A: INSTRUMENT INSTALLATION
PN 4237242C
3.1
PREINSTALLATION CHECKS, 3.1-1
Space and Accessibility Requirements, 3.1-1
Power Requirements, 3.1-1
3.2
INITIAL SETUP, 3.2-1
Unpack the Instrument, 3.2-1
Connect the Reagents, 3.2-2
Connect the Printer, 3.2-3
Enter the Initial Settings, 3.2-4
v
CONTENTS
3.3
INSTRUMENT VERIFICATION, 3.3-1
Startup the Instrument, 3.3-1
Calibrate the Instrument, 3.3-1
PART B: UPGRADE AND OPTION INSTALLATION
3.4
4
vi
OPTIONAL TICKET PRINTERS, 3.4-1
Epson TM-290P Slip Printer, 3.4-1
Purpose, 3.4-1
Single-Printer Setup Procedure, 3.4-1
Two-Printer Setup Procedure, 3.4-3
SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1
GUIDELINES, 4.1-1
4.2
ACCESSING THE HIDDEN SERVICE MENU ITEMS, 4.2-1
Accessing Service Report, 4.2-1
Purpose, 4.2-1
Procedure, 4.2-1
Accessing Service Diagnostic, 4.2-1
Purpose, 4.2-1
Procedure, 4.2-1
System Reboot, 4.2-2
4.3
REMOVING THE TOP COVER, 4.3-1
4.4
OPENING THE LOWER CHASSIS, 4.4-1
4.5
AT POWER SUPPLY, 4.5-1
Tools/Supplies Needed, 4.5-1
Removal, 4.5-1
Installation, 4.5-1
Verification, 4.5-2
4.6
AT MOTHERBOARD, 4.6-1
Tools/Supplies Needed, 4.6-1
Removal, 4.6-1
Installation, 4.6-2
Verification, 4.6-3
4.7
SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD, 4.7-1
Tools/Supplies Needed, 4.7-1
Removal, 4.7-1
Installation, 4.7-1
Verification, 4.7-2
4.8
USER RESOURCE ADAPTER (URA) CARD, 4.8-1
Tools/Supplies Needed, 4.8-1
Removal, 4.8-1
Installation, 4.8-2
Verification, 4.8-2
PN 4237242C
CONTENTS
4.9
DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS, 4.9-1
Tools/Supplies Needed, 4.9-1
Removal, 4.9-1
Installation, 4.9-2
Verification, 4.9-2
4.10 MEMBRANE KEYPAD AND DISPLAY, 4.10-1
Tools/Supplies Needed, 4.10-1
Removal, 4.10-1
Installation, 4.10-2
Verification, 4.10-2
4.11 AC POWER/VACUUM RELAY CARD, 4.11-1
Tools/Supplies Needed, 4.11-1
Removal, 4.11-1
Installation, 4.11-1
Verification, 4.11-2
4.12 +24 VOLT SWITCHING POWER SUPPLY, 4.12-1
Tools/Supplies Needed, 4.12-1
Removal, 4.12-1
Installation, 4.12-2
Verification, 4.12-3
4.13 LINEAR POWER SUPPLY CARD, 4.13-1
Tools/Supplies Needed, 4.13-1
Removal, 4.13-1
Installation, 4.13-2
Verification, 4.13-3
4.14
VACUUM SENSOR CARD, 4.14-1
Tools/Supplies Needed, 4.14-1
Removal, 4.14-1
Installation, 4.14-2
Verification, 4.14-2
4.15
MOTOR/SOLENOID DRIVER CARD, 4.15-1
Tools/Supplies Needed, 4.15-1
Removal, 4.15-1
Installation, 4.15-1
Verification, 4.15-2
4.16 SENSOR PREAMP ADAPTER (SPA) CARD, 4.16-1
Tools/Supplies Needed, 4.16-1
Removal, 4.16-1
Installation, 4.16-2
Verification, 4.16-3
4.17 PROBE AND PROBE WIPE, 4.17-1
Tools/Supplies Needed, 4.17-1
Removal, 4.17-1
PN 4237242C
vii
CONTENTS
Installation, 4.17-2
Verification, 4.17-3
4.18 PROBE MOTOR, 4.18-1
Tools/Supplies Needed, 4.18-1
Removal, 4.18-1
Installation, 4.18-1
Verification, 4.18-1
4.19 TRAVERSE DRIVE BELT, 4.19-1
Tools/Supplies Needed, 4.19-1
Removal, 4.19-1
Installation, 4.19-2
Verification, 4.19-3
4.20 TRAVERSE MOTOR, 4.20-1
Tools/Supplies Needed, 4.20-1
Removal, 4.20-1
Installation, 4.20-2
Verification, 4.20-3
4.21 SYRINGE ASSEMBLY, 4.21-1
Tools/Supplies Needed, 4.21-1
Removal, 4.21-1
Installation, 4.21-3
Verification, 4.21-4
4.22 PERISTALTIC PUMP MOTOR AND SPOOL, 4.22-1
Tools/Supplies Needed, 4.22-1
Removal, 4.22-1
Installation, 4.22-2
Verification, 4.22-2
4.23 VACUUM PUMP, 4.23-1
Tools/Supplies Needed, 4.23-1
Removal, 4.23-1
Installation, 4.23-1
Verification, 4.23-1
4.24 APERTURE, BATH AND APERTURE ELECTRODE MODULE, 4.24-1
Tools/Supplies Needed, 4.24-1
Removal, 4.24-1
Installation, 4.24-2
Verification, 4.24-3
4.25 DILUTER PANEL SOLENOIDS, 4.25-1
Tools/Supplies Needed, 4.25-1
Removal, 4.25-1
Installation, 4.25-1
Verification, 4.25-1
viii
PN 4237242C
CONTENTS
4.26 SWEEP-FLOW TUBING, 4.26-1
Tools/Supplies Needed, 4.26-1
Removal, 4.26-1
Installation, 4.26-2
Verification, 4.26-2
4.27 LATEX GAIN ADJUSTMENT, 4.27-1
Tools/Supplies Needed, 4.27-1
Procedure, 4.27-1
4.28 AIM ADJUSTMENT, 4.28-1
Tools/Supplies Needed, 4.28-1
Procedure, 4.28-1
Optional Procedures, 4.28-1
Option 1, 4.28-1
Option 2, 4.28-2
4.29 HGB PREAMP ADJUSTMENT, 4.29-1
Tools/Supplies Needed, 4.29-1
Procedure, 4.29-1
4.30 VACUUM ADJUSTMENT, 4.30-1
Procedure, 4.30-1
4.31 LYSE VOLUME ADJUSTMENT/VERIFICATION, 4.31-1
Tools/Supplies Needed, 4.31-1
Procedure, 4.31-1
5
6
7
MAINTENANCE PROCEDURES, 5.1-1
5.1
SYSTEM VERIFICATION PROCEDURE (SVP), 5.1-1
5.2
PMI RECOMMENDATIONS, 5.2-1
Components, 5.2-1
Filters, 5.2-1
Peristaltic Pump Tubing, 5.2-1
Polyurethane Tubing, 5.2-1
Syringes, 5.2-1
Cleaning, 5.2-1
Adjustment Procedures, 5.2-2
Verification Procedures, 5.2-2
SCHEMATICS AND BLOCK DIAGRAMS, 6.1-1
6.1
DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS, 6.1-1
6.2
SCHEMATICS, 6.2-1
TROUBLESHOOTING, 7.1-1
7.1
PN 4237242C
TROUBLESHOOTING DIAGNOSTICS, 7.1-1
Customer Options Available for Troubleshooting, 7.1-1
Service Menu, 7.1-2
ix
CONTENTS
8
A
x
7.2
GENERATING A SERVICE REPORT, 7.2-1
The Screen Display, 7.2-1
The Printed Report, 7.2-1
AIM Criteria Results, 7.2-2
Hgb Data, 7.2-3
Sample Results - DO NOT REPORT, 7.2-3
7.3
SERVICE DIAGNOSTIC, 7.3-1
7.4
TROUBLESHOOTING ERROR MESSAGES, 7.4-1
7.5
TROUBLESHOOTING POWER-UP PROBLEMS, 7.5-1
PARTS LISTS, 8.1-1
8.1
MASTER PARTS LISTS, 8.1-1
8.2
ILLUSTRATED PARTS, 8.2-1
QUICK REFERENCE INFORMATION, A.1-1
A.1
TOLERANCES, VOLTAGES AND LIMITS, A.1-1
Limits, A.1-1
Adjustments, A.1-1
A.2
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS, A.2-1
AC Power/Vacuum Relay Card, A.2-1
Connectors, A.2-1
Test Points, A.2-1
AT Motherboard, A.2-1
DRA Card, A.2-2
Hgb Preamp Card, A.2-2
Test Points, A.2-2
Linear Power Supply Card, A.2-2
Jumpers, A.2-2
Test Points, A.2-2
Motor/Solenoid Driver Card, A.2-3
Jumpers, A.2-3
Test Points, A.2-3
Sensor Preamp Adapter Card, A.2-4
SPAD Card, A.2-4
URA Card, A.2-4
Vacuum Sensor Card, A.2-4
A.3
PRINTER SETTINGS, A.3-1
A.4
FUNCTIONS, A.4-1
Peristaltic Pumps, A.4-1
A.5
MENU TREE, A.5-1
PN 4237242C
CONTENTS
B
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1
B.1
C
D
MESSAGE/ERROR CODE LISTINGS, C.1-1
C.1
INSTRUMENT NON-FATAL ERROR MESSAGES, C.1-1
C.2
INSTRUMENT FATAL ERROR MESSAGES, C.2-1
C.3
AMIBIOS BEEP CODES, C.3-1
OPTIONAL PRINTERS, D.1-1
D.1
E
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1
EPSON TM-290P SLIP PRINTER, D.1-1
Specifications, D.1-1
Operator Controls and LEDs, D.1-1
DIP Switch SW1 Settings, D.1-2
Installation Procedure, D.1-2
Printer Self-Test, D.1-2
INTELLIGENT SOFTWARE LINK (ISL) OPTION, E.1-1
E.1
ISL OPTION FOR RALS, E.1-1
ISL Description, E.1-1
RALS Installation, Training and Service Responsibilities, E.1-1
Recognizing an MD II Linked to RALS, E.1-1
Servicing an MD II Linked to RALS, E.1-1
TRADEMARKS
PN 4237242C
xi
C
CONTENTS
ILLUSTRATIONS
2.1-1
2.1-2
2.2-1
2.2-2
2.2-3
2.2-4
2.3-1
2.3-2
2.3-3
2.3-4
2.3-5
2.4-1
2.4-2
2.4-3
2.4-4
2.5-1
2.5-2
2.5-3
2.5-4
3.1-1
3.2-1
3.2-2
3.2-3
3.4-1
3.4-2
3.4-3
3.4-4
4.3-1
4.4-1
4.6-1
4.6-2
4.8-1
4.9-1
4.10-1
4.12-1
4.13-1
4.13-2
4.14-1
4.15-1
4.16-1
4.16-2
4.17-1
4.19-1
4.20-1
4.21-1
4.22-1
4.24-1
4.26-1
xii
Functional Diagram, 2.1-1
Software Menu Tree, 2.1-3
Top View into Upper Chassis, 2.2-3
Top View into Lower Chassis, 2.2-4
AC Power/Vacuum Relay Card, 2.2-5
Linear Power Supply Card, 2.2-7
Fluidics Panel Components, 2.3-1
Diluter Panel, 2.3-3
Probe-Wipe Traverse Assembly, 2.3-5
Peristaltic Pump Assembly, 2.3-5
Syringe Assembly, 2.3-6
AT Motherboard, 2.4-2
URA Card, 2.4-5
DRA Card, 2.4-8
Motor/Solenoid Driver Card, 2.4-12
Sensor Preamp Adapter Card, 2.5-3
Vacuum Sensor Card Block Diagram, 2.5-5
Vacuum Sensor Card, 2.5-6
SPAD Card, 2.5-10
System Electrical Connectors, 3.1-2
Reagent Connections, 3.2-2
System Electrical Connections, 3.2-3
CITIZEN GSX-190 Printer settings, 3.2-4
Epson TM-290P Slip Printer, 3.4-1
DIP Switch, SW1, Location, 3.4-2
Printer Connections for a Single Printer, 3.4-2
Printer Connections for Two Printers, 3.4-4
Top View into Upper Chassis, 4.3-1
Top View into Lower Chassis, 4.4-1
Card Removal (AT Motherboard, URA, DRAs and SPAD), 4.6-1
AT Motherboard, 4.6-3
URA Card, 4.8-1
DRA Card, 4.9-1
Membrane Keypad and Display, 4.10-1
+24 V Power Supply, 4.12-1
Linear Power Supply Card, 4.13-1
Back View of MD II with Rear Panel Door Opened, 4.13-2
Vacuum Sensor Card, 4.14-1
Motor/Solenoid Driver Card, 4.15-2
Sensor Preamp Adapter (SPA) Card and Surrounding Components, 4.16-1
Sensor Preamp Adapter Card, 4.16-3
Probe Assembly, 4.17-1
Traverse Drive Belt, 4.19-1
Traverse Motor, 4.20-1
Syringe Assembly, 4.21-2
Peristaltic Pump Motors, 4.22-1
Aperture Bath Assembly, 4.24-1
Sweep-Flow Tubing, 4.26-1
PN 4237242C
CONTENTS
7.2-1
7.2-2
7.2-3
7.5-1
7.5-2
8.2-1
8.2-2
8.2-3
8.2-4
8.2-5
8.2-6
8.2-7
8.2-8
8.2-9
A.3-1
A.5-1
D.1-1
PN 4237242C
Sample Screen Display, 7.2-1
Sample Printed Report, 7.2-2
Hgb Results Analysis, 7.2-4
Power-Up Troubleshooting Flowchart (part 1 of 2), 7.5-1
Power-Up Troubleshooting Flowchart (part 2 of 2), 7.5-2
MD II, Lower Chassis, Left Side View (See Table 8.2-1), 8.2-1
MD II, Front View (See Table 8.2-2), 8.2-2
MD II, Top View into Lower Chassis (See Table 8.2-3), 8.2-3
MD II, Upper Chassis (See Table 8.2-4), 8.2-4
MD II, Back View (See Table 8.2-5), 8.2-5
Traverse Assembly (See Table 8.2-6), 8.2-6
Diluter Assembly (See Table 8.2-6), 8.2-8
Syringe Assembly (See Table 8.2-8), 8.2-10
Peristaltic Pump Assembly (See Table 8.2-9), 8.2-12
CITIZEN GSX-190 Printer Settings, A.3-1
Software Menu Tree, A.5-1
Epson TM-290P Slip Printer Control Locations, D.1-1
xiii
C
CONTENTS
TABLES
2.1-1
2.1-2
2.1-3
2.2-1
2.2-2
2.2-3
2.3-1
2.3-2
2.3-3
2.3-4
2.4-1
2.4-2
2.4-3
2.4-4
2.4-5
2.4-6
2.4-7
2.4-8
2.5-1
2.5-2
2.5-3
2.5-4
2.5-5
2.6-1
3.1-1
3.1-2
6.1-1
6.1-2
7.1-1
7.1-2
7.3-1
7.4-1
8.1-1
8.1-2
8.1-3
8.1-4
8.1-5
8.2-1
8.2-2
8.2-3
8.2-4
8.2-5
8.2-6
8.2-7
8.2-8
8.2-9
A.1-1
A.1-2
xiv
Functions the Diluter Table Can Use, 2.1-4
Aspiration Cycle, 2.1-5
Power-Up Cycle, 2.1-6
AT Power Supply Output Connectors, 2.2-1
AC Power/Vacuum Relay Card Connectors and Line Input Ranges, 2.2-6
Linear Power Supply Card Test Points, 2.2-8
Diluter Panel Solenoids and Their Function, 2.3-4
Peristaltic Pumps Location and Function, 2.3-6
Flex Connect Card Connectors and LEDs, 2.3-7
Solenoid Interconnect Card Connectors and LEDs, 2.3-8
AT Motherboard Switch and Jumper Settings, 2.4-1
URA Card Switch and Jumper Settings, 2.4-6
DRA Card Output Connectors, 2.4-7
DRA Card Jumper Settings, 2.4-8
Motor/Solenoid Driver Card Input Connections, 2.4-10
Motor/Solenoid Driver Card Output Connections, 2.4-10
Motor/Solenoid Driver Card Test Points, 2.4-11
Motor/Solenoid Driver Card Jumper Settings, 2.4-11
Sensor Preamp Adapter Card Input Connections, 2.5-1
Sensor Preamp Adapter Card Output Connections, 2.5-2
Sensor Preamp Adapter Card Jumper Settings, 2.5-2
Vacuum Sensor Card Test Points, 2.5-6
SPAD Card Input Connections, 2.5-9
Pinouts for Serial Ports, 2.6-1
Space Requirements, 3.1-1
Connectors’ Part Numbers and Line Input Ranges, 3.1-2
DCNs and Descriptions for Schematics Included in This Manual, 6.1-1
DCNs and Descriptions for Schematics Not Included in This Manual, 6.1-1
Diluter Functions Menu Options, 7.1-1
Service Menu Options, 7.1-2
Service Diagnostic Cycle, 7.3-1
Error Messages, 7.4-1
Cables, 8.1-1
Lower Chassis, 8.1-2
Peripherals and Support, 8.1-5
Upper Chassis, 8.1-6
Miscellaneous Hardware, 8.1-7
MD II, Lower Chassis, Left Side View (See Figure 8.2-1), 8.2-1
MD II, Front View (See Figure 8.2-2), 8.2-2
MD II, Top View into Lower Chassis (See Figure 8.2-3), 8.2-3
MD II, Upper Chassis (See Figure 8.2-4), 8.2-4
MD II, Back View (See Figure 8.2-5), 8.2-5
Traverse Assembly (See Figure 8.2-6), 8.2-7
Diluter Assembly (See Figure 8.2-7), 8.2-9
Syringe Assembly (See Figure 8.2-8), 8.2-11
Peristaltic Pump Assembly (See Figure 8.2-9), 8.2-13
Calibration Factor Limits, A.1-1
Amplifier Noise Limits, A.1-1
PN 4237242C
CONTENTS
A.1-3
A.2-1
A.2-2
A.2-3
A.2-4
A.2-5
A.2-6
A.2-7
A.2-8
A.2-9
A.2-10
A.4-1
C.1-1
C.2-1
C.3-1
D.1-1
D.1-2
PN 4237242C
Adjustments, A.1-1
AC Power/Vacuum Relay Card Connectors and Line Input Ranges, A.2-1
AT Motherboard Switch and Jumper Settings, A.2-1
DRA Card Jumper Settings, A.2-2
Linear Power Supply Card Jumper Settings, A.2-2
Linear Power Supply Card Test Points, A.2-2
Motor/Solenoid Driver Card Jumper Settings, A.2-3
Motor/Solenoid Driver Card Test Points, A.2-3
Sensor Preamp Adapter Card Jumper Settings, A.2-4
URA Card Switch and Jumper Settings, A.2-4
Vacuum Sensor Card Test Points, A.2-4
Peristaltic Pumps Location and Function, A.4-1
Non-fatal Error Messages, C.1-1
Fatal Error Messages, C.2-1
AT Motherboard AMIBIOS Beep Codes, C.3-1
DIP Switch SW1 Position Settings, D.1-2
DIP Switch SW1 Settings for International Character Sets, D.1-2
xv
CONTENTS
xvi
PN 4237242C
C
CONTENTS
1
PN 4237242C
INTRODUCTION, 1.1-1
1.1
MANUAL DESCRIPTION, 1.1-1
Scope, 1.1-1
Organization, 1.1-1
Numbering Format, 1.1-1
Special Headings, 1.1-1
WARNING, 1.1-1
CAUTION, 1.1-2
IMPORTANT, 1.1-2
ATTENTION, 1.1-2
Note, 1.1-2
Conventions, 1.1-2
1.2
SAFETY PRECAUTIONS, 1.2-1
Electronic, 1.2-1
Biological, 1.2-1
Troubleshooting, 1.2-2
1-i
1
CONTENTS
1-ii
PN 4237242C
C
1INTRODUCTION 1
1.1
MANUAL DESCRIPTION
Scope
This manual provides the reference information and procedures necessary for servicing and
maintaining the COULTER® MD II™ Series analyzer.
This manual is to be used in conjunction with the appropriate customer documents and does
not contain information and procedures already covered in those documents. There are five
COULTER® MD II™ Series analyzer customer documents available for reference:
r
Host Transmission Specification, PN 4237198
r
Operator's Guide, PN 4237235
r
Quip-Tip Card, PN 4237277
r
Reference, PN 4237236
r
Training Guide, PN 4237271.
Organization
The material in this manual is organized into eight chapters. Chapter 1 includes a brief
description of this manual and essential safety information. Chapter 2 introduces the
COULTER MD II Series analyzer, hereafter referred to as the MD II or the instrument, and
describes how it functions. Chapters 3 through 5 contain the procedures for installing,
repairing and maintaining the instrument. Chapters 6 through 8 contain information required
for servicing the instrument: schematics, troubleshooting tables and a parts list. Appendices A
through C contain quick reference tables showing: instrument limits, instrument
adjustments, switch and jumper settings, and error messages/codes, and also procedures for
using the Service Diagnostics diskette.
Numbering Format
Each chapter of this manual is further divided into topics, which are numbered sequentially,
beginning at one. The numbering format for the topic heading, which is called the primary
heading, is chapter number, decimal point, topic number. For example, the primary heading
number for the fifth topic covered in Chapter 2 is 2.5.
The page, figure and table numbers are tied directly to the primary heading number. For
example, Heading 2.5 begins on page 2.5-1, the first figure under Heading 2.5 is Figure 2.5-1,
and the first table under Heading 2.5 is Table 2.5-1.
Note: Primary headings always begin on the top of a right-hand page.
Special Headings
Throughout this manual WARNING, CAUTION, IMPORTANT, Attention and Note headings
are used to indicate potentially hazardous situations and important or helpful information.
WARNING
A WARNING indicates a situation or procedure that, if ignored, can cause serious personal
injury. The word WARNING is boldfaced in the printed manual and is red in the online
manual.
PN 4237242C
1.1-1
C
INTRODUCTION
MANUAL DESCRIPTION
CAUTION
A CAUTION indicates a situation or procedure that, if ignored, can cause damage to
equipment. The word CAUTION is boldfaced in the printed manual and is red in the online
manual.
IMPORTANT
An IMPORTANT indicates a situation or procedure that, if ignored can result in erroneous
test results. The word IMPORTANT is boldfaced in the printed manual and is red in the
online manual.
ATTENTION
An ATTENTION contains information that is critical for the successful completion of a
procedure and/or operation of the instrument. The word ATTENTION is boldfaced in the
printed manual and is red in the online manual.
Note
A Note contains information that is important to remember or helpful in performing a
procedure.
Conventions
This manual uses the following conventions. An example is given below each convention.
r
Italics indicate screen messages.
The MD II screen displays ERROR CODE (018) Copy Protection Violation when the
instrument detects the wrong resource files on the Program Disk.
r
Courier font indicates text that you have to type.
When the instrument prompts you for a password, type 123.
r
Bold, all uppercase text indicates a menu option for you to select. The item number key
next to the menu item next to it, indicates the item number key you have to press to
select the menu option. The menu item is displayed on the screen but only the item
number key is on the instrument’s keypad.
From the Main Menu select 5 SPECIAL FUNCTIONS.
r
Select menu item tt sub-menu item indicates the software options you have to select, as
well as the order in which you should select them.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 4 SUPERVISOR tt
7 SERVICE DIAGNOSTICS.
r
1.1-2
In the online manual, blue, underlined text indicates a link to additional information. To
access the linked information, select the blue, underlined text.
PN 4237242C
C
INTRODUCTION
SAFETY PRECAUTIONS
1.2
SAFETY PRECAUTIONS
Electronic
WARNING Risk of personal injury. Rings or other jewelry can contact exposed electronic components
causing personal injury from electronic shock. Remove rings and other metal jewelry before performing
maintenance or service on the electronic components of the instrument.
CAUTION Risk of damage to electronic components. If removal/replacement of printed circuit card or
components is performed while power is ON, damage to components may occur. To prevent damage to
delicate electronic components, always make sure power is OFF before removing or replacing printed circuit
cards and components.
CAUTION Risk of damage to electronic components. Electrostatic discharge (ESD) can damage disk drives,
add-in circuit cards and other electronic components. Perform any procedures where there is a possibility
of ESD damage, at an ESD workstation or wear an antistatic wrist strap attached to a metal part of the
chassis that is connected to an earth ground.
Biological
WARNING Risk of personal injury or contamination. If service personnel do not properly shield themselves
while servicing the instrument with the doors open, they may become injured or contaminated. To prevent
possible injury or biological contamination, service personnel must wear gloves, a laboratory coat and eye
protection when servicing the instrument with the doors open
Use care when working with pathogenic materials. Means must be available to decontaminate
the instrument, provide ventilation, and to dispose of waste liquid. Refer to the following
publications for further guidance on decontamination.
Biohazards Safety Guide, 1974, National Institute of Health.
Classifications of Etiological Agents on the Basis of Hazards, 3d ed., June 1974, Center
for Disease Control, U.S. Public Health Service.
PN 4237242C
1.2-1
1
C
INTRODUCTION
SAFETY PRECAUTIONS
Troubleshooting
Bring the following warning to the customer's attention before advising the customer to
perform any maintenance, troubleshooting, or service procedures on their instrument.
WARNING Risk of personal injury or contamination. If operators do not properly shield themselves while
performing service, maintenance and troubleshooting procedures, residual fluids in the instrument could
injure or contaminate them. Coulter recommends that barrier protection, such as appropriate safety
glasses, laboratory coat, and gloves be worn throughout the performance of service, maintenance and
troubleshooting procedures to avoid contact with cleaners and residual fluids in the instrument.
WARNING Risk of personal injury. If non-Coulter-trained personnel attempt to service the instrument by
performing adjustment and measurements with the power ON, injury could result. Only Coulter-trained
Service personnel should service this instrument.
1.2-2
PN 4237242C
C
CONTENTS
2
PN 4237242C
2
INSTRUMENT DESCRIPTION, 2.1-1
2.1
SYSTEM OVERVIEW, 2.1-1
Description, 2.1-1
Software Menu System, 2.1-2
Software Tables, 2.1-2
Aspirate Table, 2.1-5
Power-up Table, 2.1-6
2.2
POWER SUPPLY, 2.2-1
Overview, 2.2-1
AT Power Supply, 2.2-1
Inputs, 2.2-1
Outputs, 2.2-1
Switches, 2.2-2
AC Power/Vacuum Relay Card, 2.2-2
Inputs, 2.2-5
Outputs, 2.2-5
Test Points, 2.2-5
Jumpers, 2.2-5
+24 Volt Switching Power Supply, 2.2-6
Inputs, 2.2-6
Outputs, 2.2-6
Adjustments, 2.2-6
Linear Power Supply Card, 2.2-6
Inputs, 2.2-8
Outputs, 2.2-8
Test Points, 2.2-8
Jumpers, 2.2-8
2.3
FLUIDICS PANEL, 2.3-1
Overview, 2.3-1
Diluter Panel, 2.3-2
Probe/Wipe Traverse Assembly, 2.3-4
Peristaltic Pump Assembly, 2.3-5
Syringe Assembly, 2.3-6
Individual Components Mounted to Fluidics Panel, 2.3-7
Cycle Counter and Air Solenoids, 2.3-7
Diluent Reservoir, 2.3-7
Flex Connect Card, 2.3-7
Lyse Pump (PM5), 2.3-8
Solenoid Interconnect Card, 2.3-8
Vacuum Regulator, 2.3-9
2.4
SYSTEM CONTROL, 2.4-1
AT Motherboard, 2.4-1
Switches and Jumpers, 2.4-1
User Resource Adapter (URA) Card, 2.4-2
Keypad Controller, 2.4-3
Display Controller, 2.4-3
CMOS RAM, 2.4-3
2-i
2
CONTENTS
Diagnostic Data Acquisition Circuit, 2.4-4
Utility Timer Circuit, 2.4-4
Support Circuits, 2.4-4
Inputs, 2.4-5
Outputs, 2.4-5
Adjustments, 2.4-5
Switches and Jumpers, 2.4-6
Diluter Resource Adapter (DRA) Card, 2.4-6
Motor Controller, 2.4-6
Solenoid Controller, 2.4-7
Support Circuitry, 2.4-7
Inputs, 2.4-7
Outputs, 2.4-7
Jumpers, 2.4-8Motor/Solenoid Driver Card, 2.4-8
Motor Driver, 2.4-9
Solenoid Driver, 2.4-9
Sensor Control, 2.4-9
+24 V POWER ON Signal, 2.4-9
+24 V POWERFAIL (PF/PG) Signal, 2.4-10
Inputs, 2.4-10
Outputs, 2.4-10
Test Points, 2.4-11
Jumpers, 2.4-11
2.5
2-ii
DATA ACQUISITION, 2.5-1
Sensor Preamp Adapter (SPA) Card, 2.5-1
Inputs, 2.5-1
Outputs, 2.5-2
Adjustments, 2.5-2
Jumpers, 2.5-2
Hgb Preamp Card, 2.5-3
Inputs, 2.5-4
Outputs, 2.5-4
Adjustments, 2.5-4
Test Points, 2.5-4
Vacuum Sensor Card, 2.5-4
Inputs, 2.5-5
Outputs, 2.5-5
Adjustments, 2.5-5
Test Points, 2.5-6
Sensor Processing Adapter with Diagnostics (SPAD) Card, 2.5-6
Power Supply Conditioning, 2.5-7
Oscillator Circuit, 2.5-7
Control Functions, 2.5-7
Octal Digital-to-Analog Converter (DAC), 2.5-8
Aperture Signal Processing, 2.5-8
Data Acquisition Circuit, 2.5-8
Test Pulse Generator, 2.5-9
Interrupt Selector, 2.5-9
Input, 2.5-9
PN 4237242C
CONTENTS
Outputs, 2.5-9
Jumper, 2.5-10
Adjustments, 2.5-10
2.6
PERIPHERALS, 2.6-1
Floppy Disk Drive, 2.6-1
Display, 2.6-1
Keypad, 2.6-1
Rear Panel Interface Connectors, 2.6-1
ILLUSTRATIONS
2.1-1
2.1-2
Functional Diagram, 2.1-1
Software Menu Tree, 2.1-3
2.2-1
2.2-2
2.2-3
2.2-4
Top View into Upper Chassis, 2.2-3
Top View into Lower Chassis, 2.2-4
AC Power/Vacuum Relay Card, 2.2-5
Linear Power Supply Card, 2.2-7
2.3-1
2.3-2
2.3-3
2.3-4
2.3-5
Fluidics Panel Components, 2.3-1
Diluter Panel, 2.3-3
Probe-Wipe Traverse Assembly, 2.3-5
Peristaltic Pump Assembly, 2.3-5
Syringe Assembly, 2.3-6
2.4-1
2.4-2
2.4-3
2.4-4
AT Motherboard, 2.4-2
URA Card, 2.4-5
DRA Card, 2.4-8
Motor/Solenoid Driver Card, 2.4-12
2.5-1
2.5-2
2.5-3
2.5-4
Sensor Preamp Adapter Card, 2.5-3
Vacuum Sensor Card Block Diagram, 2.5-5
Vacuum Sensor Card, 2.5-6
SPAD Card, 2.5-10
TABLES
PN 4237242C
2.1-1
2.1-2
2.1-3
Functions the Diluter Table Can Use, 2.1-4
Aspiration Cycle, 2.1-5
Power-Up Cycle, 2.1-6
2.2-1
2.2-2
2.2-3
AT Power Supply Output Connectors, 2.2-1
AC Power/Vacuum Relay Card Connectors and Line Input Ranges, 2.2-6
Linear Power Supply Card Test Points, 2.2-8
2.3-1
2.3-2
2.3-3
2.3-4
Diluter Panel Solenoids and Their Function, 2.3-4
Peristaltic Pumps Location and Function, 2.3-6
Flex Connect Card Connectors and LEDs, 2.3-7
Solenoid Interconnect Card Connectors and LEDs, 2.3-8
2-iii
2
CONTENTS
2-iv
2.4-1
2.4-2
2.4-3
2.4-4
2.4-5
2.4-6
2.4-7
2.4-8
AT Motherboard Switch and Jumper Settings, 2.4-1
URA Card Switch and Jumper Settings, 2.4-6
DRA Card Output Connectors, 2.4-7
DRA Card Jumper Settings, 2.4-8
Motor/Solenoid Driver Card Input Connections, 2.4-10
Motor/Solenoid Driver Card Output Connections, 2.4-10
Motor/Solenoid Driver Card Test Points, 2.4-11
Motor/Solenoid Driver Card Jumper Settings, 2.4-11
2.5-1
2.5-2
2.5-3
2.5-4
2.5-5
Sensor Preamp Adapter Card Input Connections, 2.5-1
Sensor Preamp Adapter Card Output Connections, 2.5-2
Sensor Preamp Adapter Card Jumper Settings, 2.5-2
Vacuum Sensor Card Test Points, 2.5-6
SPAD Card Input Connections, 2.5-9
2.6-1
Pinouts for Serial Ports, 2.6-1
PN 4237242C
C
2INSTRUMENT DESCRIPTION 2
2.1
SYSTEM OVERVIEW
Description
The MD II instrument is an automated hematology analyzer and leukocyte differential
counter For In Vitro Diagnostic Use in clinical laboratories. It is contained in one unit, with
additional space needed only for the external Printer(s), the reagents, and if used, a waste
container. The MD II is marketed as four distinct instruments; the 8 parameter and 10
parameter instruments which do not generate histograms, and the 16 parameter and 18
parameter instruments which do generate histograms. The 16 and 18 parameter instruments
have additional hardware allowing them to generate histograms.
Since the MD II is housed in one unit with indistinct physical sections, it is easier to envision
it as a set of functional or logical sections. Using this concept, the description of the unit is
divided into five sections: Power Supply, System Control, Fluidics Panel, Data Acquisition
and Peripherals.
See Figure 2.1-1 for a diagram of these sections and their relationships.
Figure 2.1-1 Functional Diagram
POWER SUPPLY
AT POWER SUPPLY
AC POWER/VACUUM
RELAY CARD
+24 V POWER SUPPLY
LINEAR POWER SUPPLY
CARD
FLUIDICS PANEL
DILUTER PANEL
PROBE WIPE/TRAVERSE
ASSEMBLY
PERISTALTIC PUMP
ASSEMBLY
SYRINGE ASSEMBLY
SYSTEM CONTROL
AT MOTHERBOARD
URA CARD
DRA1 AND DRA2 CARDS
MOTOR/SOLENOID
DRIVER CARD
DATA ACQUISTION
SENSOR PREAMP
ADAPTER CARD
Hgb PREAMP CARD
VACUUM SENSOR CARD
SPAD CARD
PERIPHERALS
FLOPPY DISK DRIVE
DISPLAY
KEYPAD
REAR PANEL INTERFACE
CONNECTORS
7242001A
PN 4237242C
2.1-1
C
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
The MD II cycle begins with the presentation of a whole-blood sample to a self-washing
aspirate probe. The MD II then aspirates 12 µL of blood, dilutes and analyzes the sample and
makes the results available to a Liquid Crystal Display (LCD) and Printer. Instrument
intelligence is provided by a program loaded at power ON from a diskette. Interaction with
the instrument is largely through use of a menu system displayed on the 4-row by 40-column
LCD and a numeric keypad, with select functions provided through dedicated keys.
Software Menu System
Figure 2.1-2 shows the software menu tree. This is the same menu tree that is shown and
described in the customer documentation except that the three hidden menu items for service
personnel are added. These hidden items, shown in bold on the diagram, are not shown on
the instrument’s display nor in the customer’s documentation. Quick steps to access these
hidden items are in Heading 4.2, ACCESSING THE HIDDEN SERVICE MENU ITEMS.
Software Tables
Instrument cycling is accomplished using diluter tables. A diluter table is a collection of
software functions. The software function performs the action requested by the table, by
enabling the appropriate electrical drivers. The table determines specifics, such as when to
perform a certain action, and for how long. For instance, to drain a bath, a solenoid valve
must be opened to open a fluid path to waste. The diluter table specifies when this valve
opens and for how long. On the MD II, a peristaltic pump is used to move waste fluids. The
function to turn the pump stepper motor on is given the step rate and the total number of
steps. The diluter table also specifies some functions that are electronic in nature. Taking a
Hgb reading and accumulating aperture data are two such functions.
This table method allows for simple changes in the diluter cycles. Since the main software
does not need to be changed, only specific numbers in a specific diluter table change. All the
other tables are unchanged, which would not be true if a change was made to the software
function.
2.1-2
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
Figure 2.1-2 Software Menu Tree
1. RUN SAMPLE
ENTER ID
ENTER RANGE
PASSWORD
CONTROL LEVEL OPTIONS
1. LOW
2. NORMAL
3. HIGH
2. RUN CONTROL
3. STARTUP
ASPIRATE
RUN CONTROL
4. REVIEW LAST SAMPLE
1. SETUP
1. DATE/TIME
2. IQAP ID #
1. SELECT DATE FORMAT
2. CHANGE DATE AND TIME
3. HOST
SETTINGS
1. HOST COMMUNICATION
2. BAUD RATE
3. DATA BITS
4. PARITY
5. STOP BITS
4. OPTIONS
1. PRINT
1. AUTOMATIC
2. MANUAL
2. CONTROL
1. COULTER CONTROL/AUTOMATED DISK
2. OTHER CONTROLS/MANUAL
3. CALIBRATION
1. COULTER CALIBRATOR/AUTOMATED DISK
2. OTHER CALIBRATOR/MANUAL
4. REPORT
FORMAT
1. FULL PAGE REPORT
2. SPLIT PAGE REPORT
3. TICKET REPORT
5. REPORT SECTIONS
1. MICROSCOPIC
2. PATIENT DEMOGRAPHICS
1. PARAMETER UNITS
3. TICKET OPTIONS
2. PARAMETER RANGES
4. HISTOGRAMS
3. PARAMETER LABELS
1. AUTO SEQUENCE ON
2. AUTO SEQUENCE OFF
5. SPECIAL
FUNCTIONS
2. REAGENT
LOT #
6. AUTO SEQUENCE
5. UNITS
3. DILUTER
FUNCTIONS
4. SUPERVISOR
1. STANDARD FORMATS
2. CUSTOM FORMATS
6. INSTITUTION NAME
1. CLEAR APERTURES
2. DISPENSE LYSE
3. DRAIN
4. RINSE
5. MIX
6. CLEAN BATHS
1. QA FUNCTIONS
2. RANGES
3. PASSWORD ACCESS
4. AUTO CALIBRATION
5. PRINT CAL FACTORS
6. CHANGE CAL FACTORS
7. NO ITEM
5. SERVICE
7. NO ITEM
PN 4237242C
1. PRINT SUMMARY
2. PRINT GRAPHS
3. PRINT ASSAY SHEET
4. PRINT ALL QC
1. RANGE 1
2. RANGE 2
3. RANGE 3
1. REPRODUCIBILITY AND CARRYOVER
2. CALIBRATION
3. PRINT CALIBRATION ASSAY SHEET
1. CBC-A
2. CBC-B
3. DIFF
4. ALL
SERVICE REPORT
1. LATEX GAIN (FOR COULTER SERVICE ONLY)
2. REPLACE SYRINGE (FOR COULTER SERVICE ONLY)
3. PULSE TEST
4. VOLTAGE READINGS
5. VACUUM ADJUST
6. REVISION LEVELS
PASSWORD
SERVICE DIAGNOSTIC
PRESENT CONCENTRATED
CLEANER TO THE PROBE
6. SHUTDOWN
7. NO ITEM
1. PRINT QA
2. PREPARE IQAP
3. MEAN TO ASSAY
4. RESTORE DEFAULTS
5. CHANGE ASSAY VALUES
6. CHANGE QC RANGES
1. PRINT RANGES
2. CHANGE RANGES
3. SELECT RANGE FILE(S)
REBOOTS SYSTEM
7242002B
2.1-3
2
C
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
The MD II has seven diluter tables or cycles. These are the aspirate, power up, startup,
shutdown, prime, latex and clean bath tables. Chapter 6 has timing charts for all the diluter
tables. Because they are the most useful in describing and troubleshooting instrument
operation, the aspirate cycle and the power-up cycle are described in detail. Make sure that
you use the most recent timing chart when troubleshooting timing concerns. Table 2.1-1
gives a list of the functions that can be used by a diluter table.
Table 2.1-1 Functions the Diluter Table Can Use
2.1-4
Motor Functions
Solenoids & Valves
S/W Requests
Asp Syringe Fill
Vac Xdcr Vent
Read Hgb Sample
Asp Syringe Disp
Vac Chmbr Vent
Read Hgb Blank #1
Dil Srng Fil
Vac Select
Read Hgb Blank #2
Dil Syringe Disp
Sweep Flow
WBC/RBC/Plt
Probe Down
RBC Aper Vac
RBC/Plt DAQ
Probe Up
WBC Aper Vac
WBC DAQ
Probe Aspirate
Bath Drain Sel
Vacuum Offset
Probe WBC
PRB Wipe Vac
Aspirate Key Wait
Probe RBC
Air Mix Select
Delay
Mix Bubbles
Waste Select
Zap Red Aperture
Drain
Bath Rinse Sel
Zap Wht Aperture
Rinse
Bath Mix Sel
Chk Dil Snsr
Dil Rsvr Fil
Prefill Select
Wait for Enter
Dil Rsvr Overfill
Dil Prefil/Dis
Probe Warn
Dil Srng Vlv
Dil Overfill
Dil Dispense
Shutdown Message
Deliver Lyse
Do CBC
Inc. Cycle #
Do Diff
Vacuum Pump
Print Results
No Selection
Rpt Activity
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
Aspirate Table
The aspirate cycle, Table 2.1-2, is the most important table for the instrument. It is used for
sample analysis during a sample, control, calibration, and reproducibility run.
Table 2.1-2 Aspiration Cycle
Time
(seconds)
Activity Requested
0.0
Move aspirate probe up and turn on vacuum pump.
1.0
Read Hgb Blank #2, move aspirate probe to aspirate position, drain WBC bath and move aspirate syringes
down.
2.5
Drain RBC bath and move aspirate probe down.
5.0
Display aspirate message and wait for Aspirate key to be pressed.
5.0
Aspirate 12 µL and rinse WBC bath.
6.0
Wipe aspirate probe while moving it up, drain WBC bath and rinse RBC bath.
8.5
Prefill WBC bath with 1.5 mL of diluent while moving aspirate probe to WBC bath.
10.0
Move aspirate probe down into WBC bath.
11.0
Take Hgb Blank #1 reading, dispense sample and diluent while draining vacuum isolator chamber (VIC).
12.0
Move aspirate probe up and mix initial WBC dilution.
14.0
Move aspirate probe down.
15.0
Aspirate 100 µL of initial WBC dilution, fill diluent reservoir, charge diluent syringe and drain RBC bath.
18.5
Move aspirate probe up.
19.5
Deliver lyse while moving aspirate probe to RBC bath and create lyse line air gap.
20.0
Move aspirate probe down while prefilling RBC bath with 0.5 mL.
21.0
Dispense RBC sample and diluent with mix bubbles.
25.0
Move aspirate probe up and apply vacuum to WBC aperture.
26.0
Apply vacuum to RBC aperture, open sweep flow and begin data accumulation.
29.0
Data accumulation takes 12 seconds with up to 24 seconds of extended count.
41.0
Turn off sweep flow and increment cycle counter.
41.5
Take Hgb readings, drain WBC bath, fill diluent reservoir and analyze data.
44.0
Rinse WBC bath, drain RBC bath, fill diluent reservoir and turn off vacuum.
46.0
Drain WBC bath, rinse RBC bath, fill diluent reservoir and begin print process.
53.0
Rinse WBC bath, drain VIC and fill diluent reservoir.
57.0
Drain VIC and overfill diluent reservoir.
60.0
Aspiration cycle is complete.
* The system extends the RBC aperture count period if there is insufficient Plt data. It uses 3-second count periods until
there is sufficient Plt accumulation or a maximum of eight extra count periods have been performed.
PN 4237242C
2.1-5
2
C
INSTRUMENT DESCRIPTION
SYSTEM OVERVIEW
Power-up Table
The power-up table, Table 2.1-3, is run by the instrument during its power-up process.
Table 2.1-3 Power-Up Cycle
Time
(seconds)
Activity Requested
0.0
Drain WBC bath. Rinse and drain 3 times consecutively. This uses about 16 mL of diluent from reservoir.
14.0
Check diluent sensor. It should not see diluent and displays ERROR DETECTED (017) Unable to Sense
Diluent Level if it does. Drain VIC and fill diluent reservoir to sensor.
80.0
Continue filling diluent reservoir if it is not filled to sensor.
80.0
Overfill diluent reservoir and check diluent sensor. Display ERROR DETECTED (017) Unable to Sense
Diluent Level if no diluent is sensed. Turn on vacuum pump and drain RBC bath.
85.0
Drain probe wipe line and prefill WBC bath from diluent syringe while draining.
90.0
Request that a probe warning be displayed, and charge diluent syringe to 3.7 mL.
93.0
Force 2.5 mL of diluent through probe wipe.
97.0
Move aspirate probe up while wiping with 1.2 mL of diluent.
99.0
Move aspirate probe to aspirate position while rinsing RBC bath.
101.0
Move aspirate probe to WBC position while filling aspirate syringe.
102.0
Move aspirate probe to RBC position, then down, then up, then drain VIC. This verifies that all Probe/Wipe
Traverse Assembly position sensors are working.
107.0
Move aspirate probe down, home aspirate syringe, move aspirate probe up.
115.0
Fill diluent syringe with 4 mL of diluent and aspirate syringe with 50 µL of diluent while draining RBC bath.
118.0
Wash aspirate probe as it moves down, dispense 50 µL of diluent from aspirate syringe, drain RBC bath.
123.0
Move aspirate probe up, charge diluent syringe to 4 mL, fill diluent reservoir.
128.0
Drain WBC bath while prefilling with 2 mL of diluent, then drain RBC bath while prefilling with 1 mL of
diluent. This primes prefill tubing.
132.0
Fill diluent syringe with 1.2 mL of diluent while moving probe down. Wash aspirate probe as it moves up,
while also draining WBC bath.
136.0
Drain VIC, apply mixing bubbles while rinsing WBC bath, drain WBC bath.
140.0
Fill diluent reservoir, then rinse RBC bath while applying mixing bubbles. Drain RBC bath and fill diluent
reservoir.
145.0
Rinse WBC bath, fill diluent reservoir, rinse RBC bath, fill diluent reservoir.
156.0
Open up aperture count and sweep-flow lines. Prime for 25 seconds.
181.0
Close RBC aperture count and sweep-flow lines. Continue priming WBC aperture module for 25 seconds
206.0
Drain VIC, turn off vacuum pump and fill diluent reservoir.
211.0
Drain WBC bath.
213.0
Rinse WBC bath, drain RBC bath, then fill diluent reservoir.
217.0
Zap apertures for 1 second and fill diluent reservoir.
228.0
Power-up cycle is complete.
2.1-6
PN 4237242C
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
2.2
POWER SUPPLY
Overview
Ac is input into the lower chassis using a connector that is also a line filter. Both lines are
fused, F1 on the hot line and F2 on the neutral line. For 120 Vac units, a 4.0-A SLO-BLO fuse
is used, and for 220 Vac units a 2.0-A SLO-BLO fuse is used. Additional conditioning is
provided by the Transient Voltage Suppressor card, a simple circuit card comprised of a gas
tube surge arrester and three varistors, connected in parallel to the incoming ac line. The ac
voltage is then made available to the AC Power/Vacuum Relay card and the AT power supply,
a PC-type computer switching supply in the upper chassis.
System power is turned on using the switch built into the AT power supply. This provides
±5 V and ±12 V to the computer in the upper chassis and any cards using the computer bus.
The User Resource Adapter (URA) card routes +5 V from the AT motherboard to the LCD
screen resulting in two solid bars being displayed. System software controls the display, so no
further screen image is seen until the system software is loaded from the Program Disk and is
executed.
Connector P2 of the AT power supply also provides +12 V directly to the AC Power/Vacuum
Relay card, energizing relay K2 and routing ac to transformer T1 and the +24 V switching
power supply. Three transformer secondary voltages provide input ac to the Linear Power
Supply card, which immediately makes available ±15 Vdc, aperture and aperture zap voltages
and the Hgb LED (Light Emitting Diode) current. When the system software has been loaded
and is in control, the Diluter Resource Adapter (DRA) card sends a command to the
Motor/Solenoid Driver card to turn on the +24 V.
AT Power Supply
The AT power supply is found in the upper chassis (see Figure 2.2-1) and is a PC-type,
200-W, switching power supply, providing ±5 V and ±12 V (Table 2.2-1). It has a built-in
cooling fan that provides air flow to the upper chassis area. Its Power On/Off switch also
serves as the instrument’s Power ON/OFF switch.
The power supply is purchased as a unit and should be replaced as a unit. Schematics and
component parts are not available.
Inputs
Standard ac line cable input connector
Outputs
Table 2.2-1 AT Power Supply Output Connectors
PN 4237242C
Connector
Type
Volts Supplied
Description
P1
P2, P3
Low-power utility
High-power utility
P4, P5
AT power supply
+5, +12
+5
+12
±5, ±12
1.0 A
1.8 A
2.8 A
To AT motherboard
2.2-1
2
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Switches
r Power On/Off switch - used as instrument’s Power ON/OFF switch
r
Ac Select switch - selects 115 or 230 Vac supply (not accessible when in unit)
AC Power/Vacuum Relay Card
The AC Power/Vacuum Relay card is found in the lower chassis (Figure 2.2-2) and takes the
ac input and distributes it. The card receives ac directly from line input and directs it for
system use. Ac into the card is first sent through relay K2. This relay directs ac to the voltage
selector plug when it is energized by +12 V, input from the AT power supply in the upper
chassis. The ac is then directed to transformer T1 for use by the Linear Power Supply card, to
the +24 V power supply, and to solid state relay K1. The ac hot (black) line to relay K1 is sent
through fuse F1, a 0.4-A, 250-V SLO-BLO fuse. A VAC ON signal of +24 V energizes K1,
which provides ac to the vacuum pump, (shown on Figure 2.2-2), turning it on.
The input and output connectors and test points are shown on Figure 2.2-3 and the line input
ranges are summarized on Table 2.2-2, at the end of this section.
2.2-2
PN 4237242C
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Figure 2.2-1 Top View into Upper Chassis
AT
POWER SUPPLY
SCREWS
COVER
SCREWS
AC POWER CABLE
DRA
CARDS
SPAD
CARD
J15 SER1
AT
POWER SUPPLY
P8
J17 FLOPPY
J18 PARALLEL
J14 SER2
URA
CARD
AT
MOTHERBOARD
P9
SW1
J20 IDE
J19
EXT BATT
FLOPPY
DISK
DRIVE
BATTERY
BANK 0
BANK 1
BANK 2
BANK 3
W3
7
4
1
_
MEMBRANE
KEYPAD/DISPLAY
PN 4237242C
8
5
2
0
9
6
3
.
7242018A
2.2-3
2
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Figure 2.2-2 Top View into Lower Chassis
90° POSITION
45° POSITION
RETAINING
SCREW
+24 V
POWER SUPPLY
MOTOR/SOLENOID
DRIVER CARD
LINEAR
POWER
SUPPLY CARD
AC POWER/
VACUUM RELAY
CARD
-V4 +
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
A
C P
O
W
E
R
/V
A
C
U
U
M
V1
+
RTN RTN
+
FLEX
CONNECT
CARD
VACUUM
SENSOR
CARD
MOTOR/SOLENOID DRIVER CARD
VACUUM
PUMP
SENSOR
PREAMP
ADAPTER
CARD
SOLENOID
INTERCONNECT
CARD
2.2-4
HGB PREAMP
CARD
7242019A
PN 4237242C
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Figure 2.2-3 AC Power/Vacuum Relay Card
TP1
(Ac in)
J6
(Line input range
selection connector)
TP2
(Ac in)
AC POWER/VACUUM
J7
(Ac out to
power transformer, T1)
ON-CARD
FUSE
J1
(AC IN)
J2
(AC OUT to
+24 V
power supply)
J3
(+12 Vdc in
from AT
power supply)
J4
(+24 V in,
VAC ON
signal)
J5
(Ac out to
vacuum pump)
7242003A
Inputs
r J1 - AC IN
r
J3 - +12 Vdc from AT power supply (PWR ON)
r
J4 - +24 V, VAC ON signal
Outputs
r J2 - AC OUT TO +24 V
r
J5 - ac out to vacuum pump (VACUUM)
r
J7 - ac out to power transformer, T1
Test Points
TP1, TP2 - ac in
Jumpers
J6 is a selection jumper connector that selects between the four possible line input ranges
(Table 2.2-2).
PN 4237242C
2.2-5
2
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Table 2.2-2 AC Power/Vacuum Relay Card Connectors and Line Input Ranges
Connector
Line Input Range
100 VOLTS
90 - 110 Vac
120 VOLTS
110 - 132 Vac
220 VOLTS
198 - 242 Vac
240 VOLTS
220 - 264 Vac
There are four plugs available to select the appropriate range. Though units are sold for a
specific power range, the other three plugs are available if the power does not match with the
plug being used.
+24 Volt Switching Power Supply
The +24 V power supply (Figure 2.2-2) is a switching power supply with a minimum
frequency of 100 kHz and a maximum rated output of 6 A at 24 Vdc. It senses and
automatically adjusts for 120 or 220 Vac input. Internal protection includes a 6.3-A fuse for
ac input, output voltage clamped at 30% overvoltage, and automatic shutdown for an
overtemperature condition.
The POWERFAIL (PF/PG) signal at J1 is monitored by the MD II. This is a TTL-compatible
signal referenced to the negative sense line at J1. A logical low indicates a fault condition and
is generated by a thermal shutdown or an ac failure longer than 38 ms.
The +24 V power supply is purchased as a unit. If it has problems, it should be replaced as a
unit, not repaired. Schematics and component parts are not available.
Inputs
r TB1 - 90 - 264 Vac, 47 - 63 Hz
r
J1 - ON/OFF signal to turn supply ON
Outputs
r TB2 - +24 Vdc
r
J1 - POWERFAIL (PF/PG) signal, internal regulator sense lines
Adjustments
V1 - This is set at the factory. Do not adjust.
Linear Power Supply Card
The Linear Power Supply card (see Figure 2.2-2 for location) provides voltages not supplied
by the AT power supply or +24 V power supply. Three ac voltages are supplied to the Linear
Power Supply card: 18.5, -18.5 and 165 Vrms (voltage root mean square). The white/orange
wires connected to J1-2 and J1-5 supply 18.5 Vrms. This is applied to bridge rectifier VR2,
producing an unregulated +25 Vdc. A regulator using an LM7815CK (U5) regulator circuit
produces the +15 V output from the card. This +15 V is also used onboard by regulator circuit
U4 to produce a 2.5-V supply that provides a constant current source for the Hgb LED.
2.2-6
PN 4237242C
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
The white/green wires connected to J1-1 and J1-4 supply -18.5 Vrms to bridge rectifier VR3.
This produces an unregulated -25 Vdc that is regulated using an LM7915CK (U6) regulator
circuit. The output of this regulator circuit is the Linear Power Supply card -15 Vdc output.
The red wire pair connected through J1-3 and J1-6 provide 165 Vrms for the aperture supply
voltages. Bridge rectifier VR1 produces about +240 Vdc from this supply. This +240 V is used
by three circuits. It is regulated to +200 V by one circuit and used for the aperture burn or zap
voltage. A CLEAR APERTURE command from the Sensor Processing Adapter with
Diagnostics (SPAD) card, entering the Linear Power Supply card at J3-8 or J3-10 (these pins
are tied together on the card), enables relay K1, which outputs the zap voltage to the
apertures. The +240 V is also used to produce the Red and White Aperture Voltage circuits.
These circuits receive gain control from the SPAD card, the red aperture current control from
pin J3-6, and the white aperture current control from pin J3-5. Performing the Latex Gain
Adjustment procedure (Heading 4.27) sets this gain.
A RED APERTURE ON command at pin J3-7, and a WHITE APERTURE ON command at
J3-4, both from the SPAD card, direct the output voltages to their respective apertures at the
appropriate time.
Test points for the Linear Power Supply card are summarized in Table 2.2-3, at the end of this
section. Figure 2.2-4 shows the location of the test points, the location and setting of the
jumpers and the location of the input and output connectors.
Figure 2.2-4 Linear Power Supply Card
J5
(RBC and WBC
aperture voltage,
zap voltage out)
J3
(Commands,
+5 V in from
SPAD card)
J2
(Hgb LED
current
supply)
X3
(OFF)
X1
(OFF)
X2
(OFF)
J4
(+15 Vdc
out)
J1
(Transformer
ac input to Linear
Power Supply card)
TP11
(Zap voltage)
TP8
TP10
(WBC aperture
voltage)
TP9
(RBC aperture
voltage)
TP7
TP6
TP5
TP4
TP3
TP2
TP1
7242004A
PN 4237242C
2.2-7
2
C
INSTRUMENT DESCRIPTION
POWER SUPPLY
Inputs
r J1 - Transformer ac input to Linear Power Supply card
r
J3 - commands, +5 V from SPAD card
Outputs
r J2 - Hgb LED
r
J4 - ±15 Vdc
r
J5 - RBC aperture voltage, WBC aperture voltage, and zap aperture voltage
Test Points
Table 2.2-3 Linear Power Supply Card Test Points
Test Point
Supply
TP1
+240 Vdc ground
TP2
+240 Vdc
TP3
-15 Vdc ground
TP4
-15 Vdc
TP5
+15 Vdc ground
TP6
+15 Vdc
TP7
Hgb LED cathode (negative lead)
TP8
Hgb LED anode (positive lead)
TP9
RBC aperture voltage
TP10
WBC aperture voltage
TP11
Aperture zap voltage (200 V)
Jumpers
X1 (GND1), X2 (GND2), X3 - These jumpers should be OFF for instrument operation. See
Figure 2.2-4 for location. They are used to provide grounding for testing the card outside of
the unit. Normal system connection provides grounding through the Sensor Preamp Adapter
card.
2.2-8
PN 4237242C
C
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
2.3
FLUIDICS PANEL
Overview
The Fluidics Panel is responsible for receiving, preparing and presenting the sample for
electronic analysis, and preparing for introduction of the next sample.
Whole-blood sample is aspirated through a sample aspirate probe, then transported to the
counting chambers. This is accomplished by the Probe/Wipe Traverse Assembly. A
self-washing mechanism is built into this assembly, relieving the operator of having to wipe
the probe.
Precision dilutions are accomplished using two syringes that make up the Syringe Assembly.
Fluidic operations requiring less precision, such as rinsing and draining, are accomplished
using peristaltic pumps. The peristaltic pumps make up the Peristaltic Pump Assembly. The
baths and the fluidic solenoids, controlling most of the fluidic activity are mounted on the
Diluter Panel. Several individual components that complete the diluter are mounted directly
to the Fluidics Panel.
The Diluter Panel, Probe/Wipe Traverse Assembly, Peristaltic Pump Assembly, Syringe
Assembly and several individual components are mounted to the Fluidics Panel
(Figure 2.3-1).
Figure 2.3-1 Fluidics Panel Components
PROBE/WIPE
TRAVERSE
ASSEMBLY
VACUUM
REGULATOR
SYRINGE
ASSEMBLY
DILUTER
PANEL
DILUENT
RESERVOIR
CYCLE
COUNTER
PERISTALTIC
PUMP
ASSEMBLY
BATH MIX
SELECT
VALVE
LV12
PN 4237242C
AIR/MIX
SELECT
VALVE
LV9
LYSE
PUMP
PM5
7242005A
2.3-1
2
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Diluter Panel
The Diluter Panel is located in the lower front of the instrument and is the main fluidic
module of the MD II. It holds all the fluid solenoid valves, the VIC, the sweep-flow tubing,
the aperture and bath components, and the Hgb LED and detector. See Figure 2.3-2. The
Sensor Preamp Adapter card is also attached to the back of the Diluter Panel through the
Aperture Electrode module cable shield. This shield provides grounding for the Diluter Panel
through the Sensor Preamp Adapter card. The Diluter Panel itself is isolated from the chassis.
The aperture system is made up of an open sample bath with an external ground electrode, an
Internal Electrode module and an aperture block that fits into the Internal Electrode module.
There are two aperture systems, one for the WBC dilution and one for the RBC/Plt dilution.
The WBC side has a 100-µ aperture similar to other COULTER instruments, except that the
aperture has been reversed to make a flatter external surface. This is done to minimize
carryover in the WBC bath. The RBC bath uses a 50-µ aperture. There is one difference in the
way these components are used in the MD II from other COULTER systems. The outside or
shield conductor of the external electrode coaxial cable is used for the signal return path. The
external or ground electrode from the bath is connected to this conductor and is fed to the
Sensor Preamp Adapter card inside a metal shield.
Hgb components are mounted directly to the WBC bath. There is an LED to supply light for
the colorimetric measuring system. It is supplied with a low constant current source and does
not generate very much heat. The light emitted from the LED is sent through a 525-nm filter.
A photodetector is mounted on the other side of the bath. The output from this detector is
sent to the Hgb Preamp inside the lower chassis.
A sweep-flow system is used by the RBC aperture to sweep red cells from the sensing zone
after they have passed through the aperture. The sweep-flow tubing is housed on a spool that
rests in a cavity below and between the baths. The cavity provides shielding, so no elaborate
canister or isolation grounding system is needed. Sweep flow on the MD II uses 13 ft of
tubing. This is possible because the diluent source is from a vented reservoir in the
instrument and independent of external reagent location.
As with other COULTER instrumentation, a VIC is used to provide constant vacuum to both
apertures while electrically isolating the electrolytic fluids drawn through the apertures. This
count vacuum is regulated at 6 in. Hg, and the vacuum sensor is directly connected to the
VIC, reducing the possiblility of inaccurate count vacuum.
The VIC is also used as a waste reservoir for the probe wipe. Raw pump vacuum of about
15 in. Hg is applied to the chamber for use by the probe wipe mechanism. How the VIC is
tubed is very important. The probe wipe produces a great deal of splashing which causes salt
bridges, eliminating the electrical isolation for the apertures. There are splash guards inside
the VIC to prevent splashing from the outside ports and the probe wipe waste should enter
these outside ports.
2.3-2
PN 4237242C
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Figure 2.3-2 Diluter Panel
15
16
1
14
2
3
13
4
12
5
11
6
10
9
1.
2.
3.
4.
5.
6.
7.
8.
WBC BATH/APERTURE
VACUUM ISOLATOR CHAMBER
RBC VACUUM VALVE (LV5)
RBC BATH/APERTURE
Hgb LED
RINSE VALVE (LV11)
DRAIN VALVE (LV7)
PREFILL VALVE (LV13)
8
7
9.
10.
11.
12.
13.
14.
15.
16.
SWEEP FLOW VALVE (LV4)
WASTE VALVE (LV10)
DISPENSE/PREFILL VALVE (LV14)
DILUENT DISPENSE VALVE (LV16)
PROBE WIPE VALVE (LV8)
DILUENT FILL VALVE (LV15)
VACUUM LOW/HIGH VALVE (LV3)
WBC VACUUM VALVE (LV6)
7242036A
PN 4237242C
2.3-3
2
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Solenoids provide the fluidic logic for the MD II. There are 12 fluidic solenoids mounted on
the Diluter Panel (Figure 2.3-2). Table 2.3-1 lists these solenoids, their name, type and
function.
Table 2.3-1 Diluter Panel Solenoids and Their Function
Solenoid
Name
Type
Function
LV3
Vac Select
3-way
Selects regulated or raw pump vacuum for the VIC.
LV4
Sweep Flow
2-way
Opens the diluent path for sweep flow.
LV5
RBC Aper Vac
2-way
Opens the RBC aperture count path to the VIC.
LV6
WBC Aper Vac
2-way
Opens the WBC aperture count path to the VIC.
LV7
Bath Drain Sel
3-way
Selects which bath drains.
LV8
PRB Wipe Vac
2-way
Opens a path from the VIC to the probe wipe housing to evacuate fluids with
high vacuum.
LV10
Waste Select
3-way
Selects whether one of the baths drains or the VIC drains.
LV11
Bath Rinse Sel
3-way
Selects which bath receives rinse from PM3.
LV13
Prefill Select
3-way
Selects which bath gets prefill from the diluent syringe.
LV14
Dil Prefil/Disp
3-way
Selects whether diluent from the diluent syringe is used for prefill or the
dispense selection of LV16.
LV15
Dil Srng Fil
3-way
Switches between the diluent reservoir for diluent syringe input and LV14
for diluent dispense output.
LV16
Dil Dispense
3-way
Switches diluent dispense between the probe wipe housing and the aspirate
probe via the aspirate syringe.
Probe/Wipe Traverse Assembly
The Probe/Wipe Traverse Assembly is located on the upper front of the instrument
(Figure 2.3-1) and is responsible for presenting the probe for whole-blood aspiration,
washing the probe after aspiration, and moving whole blood and diluted sample to the
appropriate bath.
Vertical movement is provided by a small stepper motor, the probe motor, mounted to the
traverse housing. See Figure 2.3-3. Control is provided using two optical sensors mounted on
the Probe/Opto Sensor card.
As the probe is moved up and down, it travels through the wipe housing. The wipe housing
has two ports, one with high vacuum applied, another attached to the diluent syringe. After
aspiration, as the probe is moving up, fluid is forced through the housing by the diluent
syringe as vacuum is applied. This washes the probe exterior. At several other instances of
instrument operation, with the probe moving up or down, vacuum alone is applied to dry the
probe and ensure that no dripping occurs.
Horizontal movement is provided by the traverse motor, a larger stepper motor using a belt
drive. The entire probe and probe wipe mechanism is moved as a unit to each station. This
eliminates many alignments and adjustments, producing a simple and reliable mechanism.
The only adjustment on the assembly is the spring belt tensioner for the horizontal drive belt.
This tensioner is designed in such a way that it does not need readjustment even when the
belt is replaced.
2.3-4
PN 4237242C
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Figure 2.3-3 Probe-Wipe Traverse Assembly
PROBE/
OPTO SENSOR
CARD
PROBE
MOTOR
TRAVERSE
MOTOR
TRAVERSE
HOUSING
SPRING BELT
TENSIONER
SAMPLE
ASPIRATE
PROBE
7242006A
Peristaltic Pump Assembly
The Peristaltic Pump Assembly is located on the lower left side of the instrument and consists
of a plate holding four peristaltic pumps and their associated tubing. See Figure 2.3-4.
Table 2.3-2 gives each pump’s location and function. The green and blue filters are also found
on this assembly. The green filter, a 0.45-µ fluid barrier, is found in the air lines. It blocks
fluid that might get into the line, from going any further. The blue filter, a 10-µ filter, is in the
fluid lines. The blue filter blocks particulate and air bubbles.
Figure 2.3-4 Peristaltic Pump Assembly
PM2
(DILUENT)
PM1
(AIR/MIX)
BLUE
FLUID FILTER
(10µ)
GREEN
AIR FILTER
(0.45µ)
PM3
(RINSE)
PM4
(WASTE)
7242007A
PN 4237242C
2.3-5
2
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Table 2.3-2 Peristaltic Pumps Location and Function
Pump
Location
Function
PM1 (Air/Mix)
Upper right
Provides air for isolation and mixing bubbles.
PM2 (Diluent)
Upper left
Fills the diluent reservoir from the diluent tube.
PM3 (Rinse)
Lower left
Rinses the baths with fluid from the diluent reservoir.
PM4 (Waste)
Lower right
Drains the VIC and both red and white baths.
Syringe Assembly
The Syringe Assembly is located on the right front of the instrument and consists of two
identical syringe drives with different syringe bodies. See Figure 2.3-5. Both are driven with
the same type of stepper motor that is used by the Probe/Wipe Traverse Assembly and the
Peristaltic Pump Assembly. Motion is accomplished using a belt-driven lead screw. The
lead-screw assembly, purchased as a component, is comprised of the lead screw, lead-screw
housing and backlash spring.
The left syringe drive has a 5-mL syringe mounted to it and is used for supplying diluent to
the count dilutions. A 100-µL syringe is used for sample aspiration and RBC aspiration. Input
and output control is the responsibility of solenoid valves LV14, LV15, and LV16, mounted on
the Diluter Panel.
Figure 2.3-5 Syringe Assembly
STEPPER
MOTORS
DILUENT
SYRINGE
ASPIRATION
SYRINGE
7242008A
2.3-6
PN 4237242C
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Individual Components Mounted to Fluidics Panel
The following individual components are mounted directly on the Fluidics Panel:
r
Cycle counter and air solenoids
r
Lyse pump (PM5
r
Diluent reservoir
r
Solenoid Interconnect card
r
Flex Connect card
r
Vacuum regulator.
Cycle Counter and Air Solenoids
The front left portion of the lower chassis houses the cycle counter, solenoids LV9 and LV12,
and the lyse pump PM5 (Figure 2.3-1).
Solenoids LV9 and LV12 control the air pumped from peristaltic pump PM1. LV9 selects
which bath gets mixing bubbles and LV12 routes air for the lyse line air gap.
Diluent Reservoir
The diluent reservoir is located on the left side of the instrument. See Figure 2.3-1. This is a
diluent tank into which diluent from the external source is pulled and temporarily stored.
Having a diluent reservoir in the instrument minimizes the effect of diluent height, especially
for the RBC aperture sweep flow, and the need to prime diluent after the instrument has been
sitting for a while. An added bonus is that any bubbles pulled into the reservoir from the
external diluent system dissipate in the reservoir and are not pulled into the diluter. The
reservoir has its own optical sensor, used to control the fluid level in the reservoir.
Flex Connect Card
The Flex Connect card is located in the lower chassis (Figure 2.2-2) and serves the same
interface function as the Solenoid Interconnect card. It provides a convenient connector
location for additional Fluidics Panel components and also provides an LED power indicator
for several of them.
The most prominent connector is J1, the flex cable from the Probe/Wipe Traverse Assembly.
This cable has the up and down probe sensor and probe motor wires. Table 2.3-3 shows the
various connectors and their association.
Table 2.3-3 Flex Connect Card Connectors and LEDs
PN 4237242C
Connector
Component
LED
J1
J2
J3
J4
J5
J6
J7
J8
J9
J10
Flex cable
Out to Motor/Solenoid Driver card
S10 - Waste sensor
S9 - Lyse sensor
S8 - Diluent sensor
LV9 - Air/Mix Select solenoid
LV12 - Bath Mix Select solenoid
Vacuum pump
Cycle counter
Lyse pump
No LED
No LED
No LED
No LED
No LED
CR1
CR2
CR3
CR4
CR5
2.3-7
2
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Lyse Pump (PM5)
The lyse pump (Figure 2.3-1) is a solenoid pump with a spring return. Several specifications
of this pump should be considered when troubleshooting the lyse system. It has an adjustable
working range of 200 to 450 µL and should be set to 415 ±5 µL. It cannot be energized for
more than 6 seconds or damage will result. The MD II energizes the lyse pump for 300 ms
whenever it dispenses lyse and has a fail-safe that cuts power after 1 second.
Internal sealing is accomplished using both O-rings and diaphragms. To leak back to the lyse
container, reverse pressure would have to pull through a spring pressured O-ring seal and a
diaphragm seal, similar in action, to a check valve. Forward pressure opens the diaphragm
seal by design, just as forward pressure causes flow through a check valve. Forward pressure
would also push against the spring, weakening the O-ring seal. For this reason, the pump is
rated at only 6 in. of positive pressure meaning the lyse container cannot be greater than 6 in.
above the pump.
Solenoid Interconnect Card
The Solenoid Interconnect card is located in the lower chassis (Figure 2.2-2) and provides an
interface for wiring the Diluter Panel solenoids and the horizontal probe position sensors in a
convenient location.
To facilitate servicing the instrument, LEDs were added to each solenoid line. A lit LED
indicates that its associated solenoid is being energized by the system. See Table 2.3-4 for
connector and LED associations.
Table 2.3-4 Solenoid Interconnect Card Connectors and LEDs
2.3-8
Connector
Solenoid or Sensor
LED
J1
LV6
CR1
J2
LV7
CR2
J3
LV11
CR3
J4
LV10
CR4
J5
LV3
CR5
J6
LV13
CR6
J7
LV4
CR7
J8
LV14
CR8
J9
LV8
CR9
J10
LV16
CR10
J11
LV5
CR11
J12
LV15
CR12
J13
LV2 (on Vacuum Sensor card)
CR13
J14
LV1 (on Vacuum Sensor card)
CR14
J15
S1 (WHT position)
No LED
J16
S2 (RED position)
No LED
J17
S3 (ASP position)
No LED
J18
Spare
No LED
J19
Out to Motor/Solenoid Driver card
No LED
PN 4237242C
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
Vacuum Regulator
The vacuum regulator (Figure 2.3-1) is a solid-state regulator used to regulate the 6-in. Hg
count vacuum. It is located in the upper right area of the front panel and is accessible to
customers for adjustment.
PN 4237242C
2.3-9
2
INSTRUMENT DESCRIPTION
FLUIDICS PANEL
2.3-10
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
2.4
SYSTEM CONTROL
System control is the heart of the instrument. The components that form system control
translate executing programs (software) into mechanical actions. The cards responsible for
system control are the: AT motherboard, URA, DRAs and Motor/Solenoid Driver.
AT Motherboard
The motherboard is located in the upper chassis (Figure 2.2-1) and is the primary control in
the system. It is a standard AT motherboard, using an 80386, 20-MHz CPU. BIOS is AMIBIOS
with a custom default configuration for Coulter. This ensures that the BIOS configuration
defaults to the instrument configuration if battery backup power is lost.
The motherboard’s serial and Printer connectors and the floppy disk drive controller are used
for the instrument input/output.The four Coulter system cards (URA, DRA1, DRA2 and
SPAD) are plugged into the 16-bit motherboard slots.
The motherboard’s switches and jumpers are summarized in Table 2.4-1 and Figure 2.4-1
shows the locations of the switches, jumpers and connectors.
Switches and Jumpers
Table 2.4-1 AT Motherboard Switch and Jumper Settings
Switch or
Jumper
Description
MD II Setting
SW1-1
ON - Use on-board battery
ON
OFF - Use off-board battery
SW1-2
ON - Enable battery
ON
OFF - Disable battery
SW1-3
ON - Additional wait states for IDE interface
OFF
OFF - No additional wait states
SW1-4
ON - Color adapter
OFF
OFF - Monochrome adapter (does not matter when using EGA or VGA)
W3 (Jumper) 1 to 2, 3 to 4 - 256 K or 1 MB SIMMS
1 to 2, 3 to 4
2 to 3 - 4 MB SIMMS
PN 4237242C
2.4-1
2
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Figure 2.4-1 AT Motherboard
J15
(Serial 1 port)
J15 SER1
J14
(Serial 2 port)
P8
J18
(Parallel printer
port)
J17 FLOPPY
J18 PARALLEL
J14 SER2
GROUND
SCREW
P9
J17
(Floppy disk
drive)
SW1
J19
EXT BATT
J20 IDE
SW1
BATTERY
CC MD V1.0
NYLON
MOUNTING
POST(8)
BANK 0
BANK 1
BANK 2
BANK 3
W3
W3
(1-2, 3-4)
J21
(Reset)
J22
(Speaker)
J23
(Keylock)
J24
(IDE
activity)
J26
(Turbo
SW)
J25
(Turbo
LED)
GROUND
SCREW
7242009A
User Resource Adapter (URA) Card
The URA card is located in the upper chassis (Figure 2.2-1) and its main responsibility is to
interface the system software with user resources, specifically the keypad and the display. In
addition to various support circuits, the URA card also has non-volatile CMOS RAM, a Data
Acquisition circuit and a Utility Timer circuit.
Switch and jumper settings are summarized on Table 2.4-2, Figure 2.4-2 shows the location
of the connectors, switches and jumper in addition to the jumper setting. Table 2.4-2 and
Figure 2.4-2 are at the end of the description of the URA card.
2.4-2
PN 4237242C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Keypad Controller
The heart of the keypad controller is an 8279 keyboard controller chip. This chip is operated
in "scan keyboard" mode. The keypad is a standard row and column matrix, and with the
help of an HC138 decoder used to decode the rows, the keypad controller is able to sense any
pressed keys. Communication with the keypad is through J5 (Figure 2.4-2), a 20-pin in-line
connector that receives the flex cable connector from the keypad.
Display Controller
The display controller is made up of a connector for the display, an 8-bit buffer, a
programmable logic device or GAL, and a Display Contrast circuit. The connector, J4
(Figure 2.4-2), provides the display with its data bus, command functions, power and a
contrast setting. Data is provided directly from the CPU. The GAL provides each of the eight
display command functions its own port, enabling the CPU to control the display. Two
voltages are provided for the display, +5 V for power and a variable supply to control display
contrast. Display contrast can be adjusted with R5.
The display control logic (GAL) provides support for the options switch function. By
addressing a GAL port, the CPU can read the eight-position options switch, providing a
read-only port with up to 256-coded combinations.
CMOS RAM
The URA card provides a single chip of CMOS static RAM (Figure 2.4-2). This RAM chip has
an internal battery and power management circuitry, making it non-volatile. A programmable
logic GAL is used to control this chip.
The instrument uses this memory to store all user system settings, like aperture current
voltages, print formats, calibration factors and host interface settings. The data in RAM is
actually an image of a file on the Program Disk named PD.DAT. A new Program Disk contains
default settings and if there is no data in memory, the PD.DAT file is stored in the RAM.
It is important to understand how the instrument’s software deals with these two sources of
user information, especially during power up. When the instrument software first begins
executing after loading from diskette, it strives to establish a good and uniform system of user
settings. To do this, six pieces of information are obtained.
1. The CMOS image is verified using a CRC algorithm.
2. The CMOS image’s version is checked against the loaded software version.
3. The PD.DAT file is verified using a CRC algorithm.
4. The PD.DAT file’s version is checked against the loaded software version.
5. The diskette is checked to see if it can be written to.
6. The PD.DAT file is compared to the CMOS image to see if they match.
An error is generated if:
r Both files are bad, whether they are an old version or failed the CRC.
r PD.DAT is old or failed CRC and the diskette cannot be written to.
r PD.DAT has different settings but the diskette cannot be written to.
The CMOS RAM image is copied to PD.DAT if:
r PD.DAT failed CRC.
r PD.DAT is an old version.
r PD.DAT has different settings than the CMOS RAM image.
PD.DAT is copied to CMOS RAM if:
r The RAM image failed CRC.
r The RAM image is an old version.
PN 4237242C
2.4-3
2
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Diagnostic Data Acquisition Circuit
The Diagnostic Data Acquisition circuit receives voltage data from the Vacuum Sensor, Hgb
Preamp and Sensor Preamp Adapter cards and converts this for use by the instrument
software. The supplies are input to an HI508a analog multiplexer. The CPU selects which
input to the multiplexer is output to an AD7572 A/D converter. The A/D converter outputs
digital data for the CPU, producing a digital voltage representation of medium resolution.
There are eight channels used to represent the following signals:
r
HGB VOLTAGE
r
VACUUM READING
r
WBC 26-PERCENTILE VOLTAGE
r
RBC 26-PERCENTILE VOLTAGE
r
WBC APERTURE CURRENT READING
r
RBC APERTURE CURRENT READING
r
WBC APERTURE VOLTAGE (not currently implemented)
r
RBC APERTURE VOLTAGE (not currently implemented).
Utility Timer Circuit
An 8254 programmable timer device is provided as utility timer hardware (Figure 2.4-2) for
use by the system software. This device has three timers that can all produce an interrupt. An
interrupt control latch and buffer forms an 8-bit read/write port with the timer outputs each
assigned a bit. The GAL provides the logic that controls and synchronizes the interrupts,
allowing the timers to be enabled or disabled and to be used individually or chained. The
GAL produces an IRQ15 system interrupt request.
Support Circuits
The support circuitry for the URA card consists of Power Supply Conditioning, a Bus
Interface, an Internal Bus Controller and an Oscillator circuit.
The URA receives +5 V, -5 V and +12 V from the motherboard bus connector. It also receives
+15 V and -15 V through connector J1. The +5 V, +15 V, and -15 V power supplies are fitted
with a spike supressor and a large filter capacitor when they enter the card. A bypass capacitor
is provided for the -5 V and +12 V power supplies.
The Bus Interface circuitry allows the URA card to communicate with the AT motherboard
and follows AT convention. There is a bidirectional data bus buffer, address buffer and a
command and interrupt buffer.
The URA card has its own internal data bus for the onboard logic devices. An Internal Bus
Controller is responsible for the operation of this local bus.
The AT bus provides an 8-MHz clock signal, but the URA card has its own 1-MHz Oscillator
circuit. The 1-MHz Oscillator circuit is used by the 8254 timer, the keypad controller chip
and the A/D converter chip.
2.4-4
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Inputs
r J1 - POWER
r
J2 - PVAC (VACUUM SENSOR signal)
r
J3 - AIM (from SPAD)
r
J5 - KEYBOARD
r
J6 - Hgb preamp
r
J7 - PREAMP DIAG
r
P1, P2 - AT bus edge connector
Outputs
r J4 - Display
r
P1, P2 - AT bus edge connector
Adjustments
r R5 - Display contrast
r
R6 - A/D converter zero adjustment
r
R7 - A/D converter scale adjustment
Figure 2.4-2 URA Card
J5
(KEYBOARD)
R5
(Display contrast)
PN 4237242C
J3
(AIM)
J7
(PREAMP
DIAG)
R6
(A/D zero)
J6
(Hgb)
R7
(A/D adjust)
J2
(PVAC)
J1
(POWER)
CMOS
RAM
SW1
(1-8, OFF)
P2
J4
(Display)
UTILITY TIMER
CIRCUIT
P1
X1
(ON)
7242010A
2.4-5
2
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Switches and Jumpers
Table 2.4-2 URA Card Switch and Jumper Settings
Switch or Jumper
Description
MD II Setting
SW1-1
OFF - COULTER MICRO-PAK reagent
ON - Bulk reagent
OFF
SW1-2
OFF - Does not create INF file
ON - Creates INF file
OFF
SW1-3
OFF - Normal operation
ON - Final test functions
OFF
SW1-4
OFF - Normal operation
ON - Adds Service Report when SW1-3 is ON
OFF
SW1-5 to SW1-8
Not used
OFF
X1
Connects oscillator to circuitry
ON
Diluter Resource Adapter (DRA) Card
The DRA card is located in the upper chassis (Figure 2.2-1) and is a hardware interface
between the AT motherboard and the Fluidics Panel motors and solenoids. This includes the
vacuum pump, cycle counter and lyse pump, which are treated as solenoids by the software.
There are two DRA cards used in the MD II Series and each allows the CPU to control up to
four stepper motors and 14 solenoids. To accomplish its task, the card has two motor
controllers and one solenoid controller, as well as support circuitry.
The DRA’s output connectors are summarized in Table 2.4-3 and the jumper settings are
summarized in Table 2.4-4. Figure 2.4-3 shows the location of the connectors and the
location and setting of the jumpers. Tables 2.4-3 and 2.4-4 and Figure 2.4-3 are at the end of
the description of the DRA card.
Motor Controller
The motor controller has two control circuits, each capable of interfacing with one motor and
one solenoid. Each controller has one buffered connector, two multitimers, two logic
elements and two command latches with command and status readback buffer. A motor
sensor buffer is also provided as part of the motor controller package.
The motor controller connector delivers the motor phase signals for two motors and the
energize command for two solenoids to the Motor/Solenoid Driver card. It also can receive up
to 6-sensor signals. To protect the logic elements of the Motor Controller circuit, buffers are
placed between this connector and the logic element. This provides electrical isolation from
the motor phase output and the sensor input.
A multitimer is assigned to each motor. They are 8254 programmable devices with three
timers. One timer is programmed with the motor step rate or frequency, the second is
programmed with the number of steps, and the third timer is unused.
The logic control for each motor is a GAL 22v10. This device takes parameter input from the
CPU for the motor rate, direction step mode and sensor mode (STOP on sensor or not). It
also receives commands to STOP or ENABLE a motor, or not and can receive input from one
sensor. This input allows the GAL to produce phase signals, control power, and generate an
interrupt when a motor task is completed. The end of task interrupt (IRQ11 for DRA1 and
IRQ12 for DRA2) is generated when sensor has been reached or the 8254 timer has counted
the steps that it was programmed with.
2.4-6
PN 4237242C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Commands to the GAL are provided by an 8-bit latch. Seven bits of this latch represent the
parameters SENSOR MODE, STOP, ENABLE, SENSOR ENABLE, DIRECTION, STEP and
POWER. The eighth bit does not go to the GAL. It is passed through a buffer to the circuit
connector and is used to control one solenoid. The output of the latch is also attached to a
unidirectional bus buffer. By enabling this buffer, the host CPU can read the command latch.
A motor sensor buffer is also provided by each Motor Controller circuit. This is an 8-bit buffer
that can be read by the CPU. Six bits can be used for sensors and two bits are assigned for
interrupts. Each GAL is assigned one of the sensors, should it require sensor information to
control a motor.
Solenoid Controller
The solenoid controller is a third Motor Controller circuit adapted to handle only solenoids.
The latch bits for STOP, ENABLE, DIRECTION, and STEP modes are now used to represent
four solenoids. The GAL is programmed to pass these ENABLE signals through to the four
lines used for the stepper motor phases. Since each control circuit of a motor controller
already handled one solenoid, the entire solenoid controller can control 10 solenoids.
Support Circuitry
The support circuitry for the DRA card consists of Power Supply Conditioning, a Bus
Interface, an Internal Bus Controller, and the Oscillator circuit. The DRA card uses +5 V from
the motherboard bus connector. This supply is fitted with a spike supressor and a large filter
capacitor when it enters the card. A bypass capacitor is also provided for the input line of
each IC.
The Bus Interface circuitry allows the DRA card to communicate with the AT motherboard
and abides by the AT convention. There is a bidirectional data bus buffer, address buffer, and a
command and interrupt buffer.
The DRA card has its own internal data bus for the onboard logic devices. An Internal Bus
Controller is responsible for the operation of this local bus.
The AT bus provides an 8-MHz clock signal, but the DRA card has its own 1-MHz Oscillator
circuit. This 1-MHz Oscillator circuit is used exclusively by the four, 8254 motor control
timers.
Inputs
P1, P2 - AT bus connector
Outputs
Table 2.4-3 DRA Card Output Connectors
PN 4237242C
Connector
Connected To
J1
Motor/Solenoid Driver card
J2
Motor/Solenoid Driver card
J3
Motor/Solenoid Driver card
P1, P2
AT bus connector
2.4-7
2
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Jumpers
Table 2.4-4 DRA Card Jumper Settings
Jumper
Description
MD II Setting
X4
Sets card for IRQ11, needed for DRA1
1-3
Sets card for IRQ12, needed for DRA2
2-3
Sets card to DRA1 or DRA2
ON - DRA1
X5
OFF- DRA2
X6
Connects oscillator to circuit when jumped
ON
Figure 2.4-3 DRA Card
TO
MOTOR/SOLENOID
DRIVER CARD
J3
J2
X5
(Sets card
for #1 or #2)
X4
(Sets IRQ
for 11 or 12)
X6
(ON)
J1
7242011A
Motor/Solenoid Driver Card
The Motor/Solenoid Driver card is located in the lower chassis (Figure 2.2-2). It receives
direction from the DRA cards in the upper chassis and distributes power to Fluidics Panel
components, such as motors, solenoids, and the vacuum pump. It also provides an overload
timer for the +24 V power supply, power for the instrument sensors, and logic to control the
lyse solenoid pump, the POWERFAIL (PF/PG) signal, and the +24 V POWER ON signal.
The Motor/Solenoid Driver card’s input connectors are summarized in Table 2.4-5, the output
connectors are summarized in Table 2.4-6, the test points are summarized in Table 2.4-7, the
jumper settings are summarized in Table 2.4-8 and the locations of the connectors and
jumpers are shown on Figure 2.4-4. Tables 2.4-5 through 2.4-8 and Figure 2.4-4 are at the
end of the description of the Motor/Solenoid Driver card.
2.4-8
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Motor Driver
All motors use the same Motor Driver circuit, which uses a UDN2878W driver device. Four
phase signals are input from the DRA card(s). The driver outputs four +24 V lines to the
motor in the phases set by the DRA card(s).
An Overload Timer circuit on the Motor/Solenoid Driver card offers protection to the motors
and drivers. The +24 V, which is received by the Motor Driver circuit’s 25-W resistors, is
delivered through Q3, a 10-A transistor. The base of Q3 is controlled by the output of an
LM339 comparator. When +24 V is directed to a motor, it is also presented to an RC timer (R1
and C1). C1 is input to the comparator along with +16 V, and when C1 charges higher (about
2 minutes), Q3 stops conducting. This cuts off the +24 V power supply from the motor
drivers. The +24 V overload latch is also set, which allows the instrument software to detect
that an overload occurred.
Solenoid Driver
The command to energize a solenoid reaches the Motor/Solenoid Driver card by way of a DRA
card. These signals are received by the card and latched using a 74HCT540 device. The
output of the latch for most solenoids is fed directly into a ULN2823 driver device that
applies +24 V to the designated solenoid.
The lyse pump is one exception. The LYSE PUMP signal from the latch is first passed through
a programmable logic device before entering the ULN2823 driver device. This logical circuit
enables a timer that de-energizes the lyse pump after 1 second.
Four solenoids, LV6, LV7, LV10, and LV11 use a UDN2878W driver device. This is a motor
driver that was used to energize larger solenoids in earlier versions of the instrument.
Sensor Control
All the system sensors are tied to the Motor/Solenoid Driver card. They are provided with a
current source for the LED and +5 V for the detector. The detector output is received and
made available to the proper DRA card. Most sensor signals are sent directly to the DRA card,
but the traverse sensors are treated differently. The traverse has two motors which STOP on
sensor and five sensors. The motor driver logic on the DRA card can take input from only one
sensor for each motor. A multiplexer is used to select the proper sensor signal for output to
the DRA card. The three horizontal position sensors are multiplexed for use by the traverse
motor and the two vertical sensors are multiplexed for use by the probe motor. The
Multiplexer circuit is implemented as part of the programmable logic device.
+24 V POWER ON Signal
The +24 V power supply has a POWER ON signal input. When this signal is high or floating
(disconnected), the power supply is turned on. When the input is low or grounded, the
power supply will not output +24 V. The MD II Series uses this feature and connects a
POWER ON signal from the Motor/Solenoid Driver card.
When the software loads and begins executing, a command is sent from the DRA card to the
Motor/Solenoid Driver card to turn on the +24 V power supply. This command is processed
by a programmable logic device, U21, which sets an output latch. The output of the latch is
inverted (low when the latch is set) and applied to the base of transistor Q4. If the base of Q4
is low, Q4 does not conduct, which causes the line to float and turn on the +24 V. There is a
jumper, X1 (Figure 2.4-4), in this line as well. If it is removed, the +24 V power supply always
turns on.
PN 4237242C
2.4-9
2
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
At power up, the programmable logic device is reset, which clears all latches. This causes Q4
to conduct, grounding the POWER ON signal. The system remains in this state with the
+24 V off until the instrument’s software is loaded and turns on the +24 V.
+24 V POWERFAIL (PF/PG) Signal
The +24 V power supply outputs a POWERFAIL signal. This signal indicates failure to
produce +24 V within factory-set limits, a brownout of at least 38 ms, or a temperature
overload on the supply. The MD II Series monitors this signal. The signal is connected to a
latch on the Motor/Solenoid Driver card. This latch is part of a programmable logic device
and is polled by the instrument software when it does a +24 V power supply check. It is
important to realize that the latch remains set even after the condition that caused it to be set
goes away.
Inputs
Table 2.4-5 Motor/Solenoid Driver Card Input Connections
Connector
Connected To
J1
DRA2, J1
J2
DRA2, J2
J3
DRA2, J3
J4
DRA1, J1
J5
DRA1, J2
J6
DRA1, J3
J15
+24 V, POWER ON, POWERFAIL
J18
Resistor bank
J23
Resistor bank
Outputs
Table 2.4-6 Motor/Solenoid Driver Card Output Connections
2.4-10
Connector
Connected To
J7
Solenoid Interconnect card
J8
Peristaltic pump motors
J10
Syringe Assembly and traverse motor
J11
Flex Connect card
J12
Not used
PN 4237242C
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Test Points
Table 2.4-7 Motor/Solenoid Driver Card Test Points
Test Point
Supply
TP1
Overload Timer input to comparator
TP2
Overload Timer output
TP3
Common ground
TP4
Lyse sensor output
TP5
Overload timer reference (16 V)
TP6
+24 V supply
TP7
Waste level output
TP8
Probe-wipe down sensor output
TP9
Probe-wipe upper sensor output
TP10
Diluent sensor output
TP11
Spare sensor output
TP12
Probe WBC position sensor output
TP13
Probe aspirate position sensor output
TP14
Probe RBC position sensor output
TP15
Aspirate syringe sensor output
TP16
Diluent syringe sensor output
TP17
POWER ON signal (to +24 V supply)
TP18
Overload Timer +24 V input supply
TP19
Spare sensor output
TP20
Spare sensor output
TP21
Spare sensor output
TP22
Spare sensor output
TP23
Oscillator output
Jumpers
Table 2.4-8 Motor/Solenoid Driver Card Jumper Settings
Jumper
Description
MD II Setting
X1
+24 V control
ON
X2
ON - Connects oscillator to circuit
ON
OFF - Disconnects oscillator for card testing
PN 4237242C
2.4-11
2
C
INSTRUMENT DESCRIPTION
SYSTEM CONTROL
Figure 2.4-4 Motor/Solenoid Driver Card
J4
(To/from
DRA1, J1)
J18
(Resistor
bank)
J5
(To/from
DRA1, J2)
J2
(To/from
DRA2, J2)
J23
(Resistor
bank)
J15
(To +24 V
POWER ON)
J1
(To/from
DRA2, J1)
X1
(ON)
J6
(To/from
DRA1, J3)
J3
(To/from
DRA2, J3)
J11
(To/from Flex
Connect
card)
J8
(To
peristaltic
pump
motors)
J10
(To/from
Syringe
Assembly/
traverse
motor
J7
(To Solenoid
Interconnect card)
J12
(Not used)
MOTOR/SOLENOID DRIVER CARD
X2
(ON)
7242012A
2.4-12
PN 4237242C
C
INSTRUMENT DESCRIPTION
DATA ACQUISITION
2.5
DATA ACQUISITION
The data acquisition section is responsible for the accumulation and measurement of system
data that is used to determine final system output (instrument results). The data acquisition
section includes the:
r
r
r
r
Sensor Preamp Adapter (SPA) card
Hgb Preamp card
Vacuum Sensor card
SPAD card.
Sensor Preamp Adapter (SPA) Card
The Sensor Preamp Adapter card is located in the lower chassis (Figure 2.2-2) and has three
distinct circuits:
r
r
r
The Preamp circuit
The Dc Restorer circuit
The High-gain Stage circuit.
Signals are input from the RBC and WBC aperture sensors in the form of current pulses.
These pulses are preprocessed by the Sensor Preamp Adapter card and then passed on to the
SPAD card for further processing.
The first stage of the Sensor Preamp Adapter card is the Preamp circuit. This circuit provides
a constant current source for the aperture system, and changes in aperture resistance produce
voltage pulses. In effect, the Preamp is a current-to-voltage converter that senses changes in
the current source it provides and passes voltage pulses to the Dc Restorer circuit. An aperture
zap of ≈200 V, supplied by the Linear Power Supply card, is applied through this stage to
dynamically clean the apertures.
The Dc Restorer circuit receives these voltage pulses and provides the proper dc offset
required by the SPAD card. This circuit ensures that the voltage output will always be
positive.
The last stage of the Sensor Preamp Adapter card is a times-eight signal conditioner, which
produces a suitable signal amplitude to pass on to the SPAD card.
Input connections for the Sensor Preamp Adapter card are summarized in Table 2.5-1, output
connections are summarized in Table 2.5-2, and jumper settings are summarized in
Table 2.5-3. Figure 2.5-1 shows jumper locations and settings and the location of input and
output connectors.
Inputs
Table 2.5-1 Sensor Preamp Adapter Card Input Connections
PN 4237242C
Connector
Description
J1
J2
J7
J8
J10
High voltage supply
Analog power entry (from Linear Power Supply card)
Diagnostics
WBC APERTURE signal
RBC/PLT APERTURE signal
2.5-1
2
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Outputs
Table 2.5-2 Sensor Preamp Adapter Card Output Connections
Connector
Description
J3-J6
Analog power distribution (out)
J9
WBC signal
J11
RBC signal
J12
PLT signal
Adjustments
There are four adjustable resistors on this card. They are adjusted at the factory to set dc
levels in the Preamp and Dc Restorer stages. The adjustment requires a test setup that is
impractical for field procedures.
Jumpers
Table 2.5-3 Sensor Preamp Adapter Card Jumper Settings
2.5-2
Jumper
Description
MD II Setting
X3
Grounds WBC Dc Restorer input for subassembly adjustment and testing.
OFF
X4
Connects WBC Preamp output to Dc Restorer circuit.
ON
X7
Connects RBC Preamp output to Dc Restorer circuit.
ON
X8
Grounds RBC Dc Restorer input for subassembly adjustment and testing.
OFF
PN 4237242C
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Figure 2.5-1 Sensor Preamp Adapter Card
J1
(From Linear
Power Supply
card)
J2
(From Linear
Power Supply
card)
J3
(Out to
URA)
J4
(Out to
SPAD)
J5
(Analog
voltage
spare)
J6
(Analog
voltage
spare)
J7
(Diagnostics)
X7
(ON)
X8
(OFF)
J11
(RBC signal
out to SPAD)
J12
(PLT
signal
out to
SPAD)
J10
(On back
of card)
J9
(WBC
signal
out to
SPAD)
R8
J8
(On back
of card)
R7
X4
(ON)
X3
(OFF)
7242013A
Hgb Preamp Card
The Hgb Preamp card is located in the lower chassis (Figure 2.2-2). It is a hemoglobin
detector amplifier producing the HEMOGLOBIN signal for the URA card. The Hgb Preamp
card is a variable gain (to 11 times) amplifier and a current-to-voltage converter. It takes the
current signal from the Hgb detector mounted to the WBC bath, amplifies it and converts it,
then sends it to the URA card. An A/D converter on the URA card makes the information
available to the system program.
There are two adjustments built into the amplifier, one for adjusting overall gain and one for
adjusting the amplifier offset. The gain adjustment compensates for optical differences in the
light path and optical components. It assures an output voltage to the URA card that provides
maximum resolution of the A/D converter. The offset adjustment is used to compensate for
PN 4237242C
2.5-3
2
INSTRUMENT DESCRIPTION
DATA ACQUISITION
dark current from the detector. There is interaction between these two adjustments, so they
must be adjusted together.
Inputs
r J1 - Hgb photodetector
r
J2 - ±15 V
Outputs
r J2 - Hgb output voltage
r
J3 - Hgb lamp output: currently not used
Adjustments
r R7 - Hgb Preamp adjustment (adjusts output voltage)
r
R8 - Preamp offset adjustment (adjusts Hgb zero)
Test Points
r TP1 - orange, Hgb Preamp card output
r
TP2 - black, Hgb Preamp card ground
Vacuum Sensor Card
The Vacuum Sensor (Vacuum Sense/Vent Valve) card is located in the lower chassis
(Figure 2.5-2) and provides a means of accurately reading count vacuum. It accepts a vacuum
input from 0.0 in. Hg to 7.0 in. Hg and converts it to an electrical signal from 0.0 V to 5.0 V.
The card also is host to two solenoid valves, LV1 and LV2. LV1 connects the vacuum sensor to
the VIC or to atmosphere. LV2 controls the VIC vent to atmosphere, which is required when
the chamber is being drained. A block diagram showing the functioning of the Vacuum
Sensor card is shown in Figure 2.5-2.
The main component of the Vacuum Sensor card is U1, a differential pressure transducer that
outputs a voltage relative to the pressure difference at its input ports, P1 and P2. Port P1 in
the MD II implementation is always open to atmosphere, while P2 is connected to LV1, which
determines the pressure source to be measured. The voltage output of U1 is fed through a
low-pass filter and amplifier section that filters out electrical noise and sets the gain. This
voltage is fed through a summing amplifier along with the offset reduction source voltage to
produce the final VAC OUT voltage level.
The offset reduction source is a negative voltage close to 1.0 V. Since the pressure transducer
outputs about 1.0 V when there is zero pressure differential, the offset reduction lowers this
output to near, but greater than, 0.0 V, producing better resolution with the expected output
range of 0.0 V to 5.0 V.
To measure vacuum, LV1 is first energized, which connects P2 to atmosphere. A reading is
taken that establishes the zero reference voltage. LV1 is then de-energized, connecting P2 to
the VIC, and the high reference voltage reading is taken.
2.5-4
PN 4237242C
INSTRUMENT DESCRIPTION
DATA ACQUISITION
The vacuum measurement is the difference between these two reference readings. During a
sample analysis cycle, one zero reference is taken prior to the first count period, and a high
reference voltage reading is taken for each of the 12 count periods. This produces a vacuum
measurement for each of the count periods.
Figure 2.5-2 Vacuum Sensor Card Block Diagram
8.000 V
REFERENCE
VOLTAGE
SOURCE
PRESSURE
TRANSDUCER
+24 V
SIG1
LV1
OFFSET
REDUCTION
SOURCE
LOW PASS
FILTER
GAIN
ADJUST
+
SUMMING
AMPLIFIER
+24 V
SIG2
LV2
VAC
OUT
7242045A
The input and output connectors and test points for the Vacuum Sensor card are shown in
Figure 2.5-3 and the test points are summarized in Table 2.5-4.
Inputs
r J1 - ±15 V
r
J2 - solenoid LV1
r
J3 - solenoid LV2
Outputs
J1 - VAC OUT
Adjustments
R2 - The Gain adjustment calibrates the card and requires an accurate vacuum measuring
device to set it. It is a factory adjustment that cannot and must not be adjusted in the field.
PN 4237242C
2.5-5
2
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Test Points
Table 2.5-4 Vacuum Sensor Card Test Points
Test Point
Description
TP1
8.006 V reference voltage (7.964 V to 8.049 V)
TP2
Transducer output
TP3
Gain adjustment output
TP4
Ground
TP5
VAC OUT
Figure 2.5-3 Vacuum Sensor Card
TEST
POINTS
(1-5)
J3
(To
solenoid
LV2)
J2
(To
solenoid
LV1)
J1
(+ 15 V in
VAC OUT)
TP1
7242014A
Sensor Processing Adapter with Diagnostics (SPAD) Card
The SPAD card is located in the upper chassis (Figure 2.2-1). Its primary function is to
acquire and process instrument data. The SPAD card is a full-size, AT-type card designed to
plug into a 16-bit slot in the AT motherboard.
The SPAD card acquires WBC, RBC, and Plt pulse data from the Sensor Preamp Adapter card.
This data is counted, edited, channelized, and converted to digital format. It is then made
available to the processing software via the AT bus interface.
2.5-6
PN 4237242C
INSTRUMENT DESCRIPTION
DATA ACQUISITION
The SPAD’s input connections are summarized in Table 2.5-5 and their location is shown on
Figure 2.5-4. Figure 2.5-4 also shows the location and setting of the jumper. Table 2.5-5 and
Figure 2.5-4 are at the end of the description of the SPAD card. The card is comprised of the
following circuitry.
Power Supply Conditioning
This card receives +5 V from the +24 V power supply through the AT bus slot and ±15 V from
the Linear Power Supply card through connector J4. These regulated supplies are further
conditioned by the SPAD card with a Spike Suppressor circuit and a large filter capacitor. In
addition, each IC on the card has a bypass filter capacitor.
Oscillator Circuit
A 16-MHz, onboard oscillator is directed into the three programmable logic devices, U22,
U23 and U33. These devices buffer, divide, use and supply the timing signals for other
circuits on the card.
Control Functions
Card housekeeping consists of several logical functions, an AT Bus Interface, an Internal Bus
Controller, the Main Port Address Decoder, Control Port 1, and Control Port 2. These circuits
or functions do not use discrete components or specialized chips. They are programmed on
chip U23, labeled ASPAD, which is an Electrically Programmable Logical Device (EPLD).
The AT Bus Interface provides the circuitry allowing communication to and from the AT
motherboard. It is comprised of a bidirectional data buffer, an address buffer, and the
command and interrupt buffer and conforms to AT slot specifications. There is also an
Internal Bus Controller.
The SPAD card has its own data bus. The Internal Bus Controller is the controller for this
internal bus.
The Main Port Address Decoder supplies the chip select signals for all the chips on the
SPAD card.
Control Port 1 is a Read/Write port providing four control signals/commands:
r
r
r
r
SELECT (for Red/White 26-percentile sync)
CLEAR (the state machine)
INTERRUPT ENABLE (DMA terminal count)
INTERRUPT ENABLE (8-channel DAS)
Control Port 2 is a Read/Write port providing eight control signals/commands:
r
r
r
r
r
r
r
r
PN 4237242C
APERTURE CURRENT ON/OFF
RED APERTURE SELECT
WHITE APERTURE SELECT
APERTURE CLEAR
EDITOR ENABLE/DISABLE
PRECHARGE ON/OFF
COUNTER CLEAR
COUNTER ENABLE
2.5-7
2
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Octal Digital-to-Analog Converter (DAC)
The octal DAC contains eight identical DACs. They are attached to a common reference but
are individually latched. They provide:
r
r
r
r
r
RBC aperture current voltage setting (coarse and fine)
WBC aperture current voltage setting (coarse and fine)
RBC counting threshold
WBC counting threshold
Plt lower and upper thresholds.
Aperture Signal Processing
The signal processing functions are programmed into another EPLD. This programming
actually spans two chips, U22 and U33. They are labeled BSPAD, and contain the WBC
processor and the RBC/Plt processor. These processor functions are capable of running
concurrently, allowing information from the RBC and WBC baths to be processed at the same
time, speeding up the instrument cycle.
The WBC processor counts and channelizes pulses that it receives from the WBC preamp (on
the Sensor Preamp Adapter card). As they are received by the processor, all pulses are sent to
the channelizing section, but only pulses that exceed a threshold are sent to the 17-bit
counter. This threshold is set by the system software and allows pulses representing particles
greater than 35 fL to be counted. Pulses sent to the channelizing section are first edited. The
editor issues a "good pulse" strobe based on the shape, size, and width of the pulse. If a pulse
is judged to be good, it is peak detected and applied to the input of an A/D converter. The
8-bit output of the A/D converter is then transferred directly to memory and forms the
channelized data or histogram.
The RBC/Plt processor counts and channelizes pulses that it receives from the RBC and Plt
preamp (on the Sensor Preamp Adapter card). RBC pulses received by the processor are
sorted according to thresholds established by the CPU. Pulses that exceed a threshold
representing 36 fL are sent to the 17-bit counter where they are counted as RBC cells.
Pulses that fall into a threshold range representing 2 to 20 fL are considered Plts and are peak
detected and sent to an A/D converter. The peak detector is used to hold the peak amplitude
until the A/D process is finished. The output of the A/D converter designates a memory
location to be incremented, creating the Plt histogram as a series of memory locations. All
pulses not considered Plts are sent to the RBC editor and channelizing section. The editor
issues a "good pulse" strobe based on the height, shape and width of the pulse. If a pulse is
judged to be good, it is peak detected and applied to the input of an A/D converter. The 8-bit
output of the A/D converter is then transferred directly to memory and forms the channelized
data or RBC histogram.
Data Acquisition Circuit
There is a Data Acquisition circuit on the SPAD card that is used to monitor the +5 V, +12 V
and +15 V power supplies. They are monitored using the system software DVM function. The
Data Acquisition circuit is contained on chip U39. It is an eight-channel device with the
following instrument data assigned to three of the eight channels:
r
r
r
r
2.5-8
+5 V supply
+12 V supply
+15 V supply
Five channels are currently not used.
PN 4237242C
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Test Pulse Generator
A test pulse generator has been established on the SPAD card with two ICs. U40 is a logic chip
that establishes the pulses and U38 is the A/D converter used by the logic chip. The MD II
uses this test pulse generator to create a RAMP pulse train during the PULSE TEST accessed
from the Service menu. The SPAD card processes this pulse train and generates a report. If
Auto Print is active, histograms are also displayed.
Interrupt Selector
The interrupt selector is responsible for signaling the CPU with an interrupt request and
determining what interrupt level to use.
Input
Table 2.5-5 SPAD Card Input Connections
Connectors
Connected To
P1, P2
AT bus edge connector (card uses +5 V from the AT bus)
J1
PLT PREAMP signal
J2
RBC PREAMP signal
J3
WBC PREAMP signal
J4
±15 V
J5
Voltage monitor (for future use)
J8
10-pin power control
J9
RBC and WBC AIM voltage (to the URA card)
Outputs
P1, P2 - AT bus edge connector
PN 4237242C
2.5-9
2
INSTRUMENT DESCRIPTION
DATA ACQUISITION
Figure 2.5-4 SPAD Card
X7
(ON)
J9
(AIM to URA)
J8
(10-pin
power
control)
J4
(+15 V in)
J3
(WBC
PREAMP
signal in)
W
R
J2
(RBC
PREAMP
signal in)
P
J1
(PLT
PREAMP
signal in)
P2
(AT bus
edge connector)
J5
(Not used)
P1
(AT bus
edge connector)
7242015A
Jumper
X7 (Set to ON)
r
ON - Connects oscillator to circuit
r
OFF- oscillator for card testing
Adjustments
There are two potentiometers onboard, R85 and R86. These are factory adjustments affecting
the Editor circuit and require an oscilloscope and pulse generator to set them. They cannot
and must not be adjusted in the field.
2.5-10
PN 4237242C
C
INSTRUMENT DESCRIPTION
PERIPHERALS
2.6
PERIPHERALS
Floppy Disk Drive
The floppy disk drive is a standard 3.5 in., 1.44 MB, Double-sided High-density drive. The
main system program is loaded from this drive on power up or reboot. A program disk is used
to power up the instrument, but a calibration or control disk may be requested. Software
updates merely require that the new program disk be used at power ON.
Display
Visual output is provided by a 4-row by 40-column, character only LCD. This display
(Figure 2.2-1) has an operating temperature range of 0 to 50°C. Electrical input consists of
one 16-pin connector, connected through a ribbon cable to the URA card. Pins 1 through 8
are the data bus, pins 9 through 12 are the control lines, pin 13, V0, is the display contrast
voltage, pin 14, VDD, is the +5 Vdc power supply, and pins 15 and 16 are the ground pins.
The display is a component piece purchased from an outside vendor, so low-level
troubleshooting is not an option.
Keypad
The MD II keypad (Figure 2.2-1) is a 36-switch, non-tactile, membrane keypad with a
graphic overlay. The MD II makes use of only 24 switches. All switches are environmentally
sealed with an operating temperature range of +10 to +35°C. Switch selection uses a standard
row/column matrix methodology, output through a 20-pin flex ribbon cable to the URA card.
A separate flex cable for the built-in electrostatic shield is connected to the chassis for
grounding.
Both cables are part of the component as purchased and cannot be changed separately.
Rear Panel Interface Connectors
There are three interface connectors on the rear panel, Serial 1, Serial 2 and the Parallel
Printer connector (Figure 3.2-2). All three connectors attach directly to the AT motherboard.
Serial 1 is a 25-pin, RS-232 serial connector and Serial 2 is a 9-pin, RS-232 serial connector.
Serial 1 is used for the ASTM host interface while Serial 2 is unused. The Parallel Printer
connector is a standard Centronics 25-pin output connector that is used for the system
Printer. Pinouts for the serial ports are shown in Table 2.6-1.
Table 2.6-1 Pinouts for Serial Ports
PN 4237242C
Function
Direction
Serial 1 (25 Pin)
Serial 2 (9 Pin)
No Connection
N/A
1 (shield)
N/A
Transmitted Data
From MD II
2
3
Received Data
To MD II
3
2
Request To Send
From MD II
4
7
Clear To Send
To MD II
5
8
Data Set Ready
To MD II
6
6
Signal Ground
N/A
7
5
Data Terminal Ready
From MD II
20
4
Ring Indicator
To MD II
22
9
2.6-1
2
INSTRUMENT DESCRIPTION
PERIPHERALS
2.6-2
PN 4237242C
C
CONTENTS
3
INSTALLATION PROCEDURES, 3.1-1
PART A: INSTRUMENT INSTALLATION
3.1
PREINSTALLATION CHECKS, 3.1-1
Space and Accessibility Requirements, 3.1-1
Power Requirements, 3.1-1
3.2
INITIAL SETUP, 3.2-1
Unpack the Instrument, 3.2-1
Connect the Reagents, 3.2-2
Connect the Printer, 3.2-3
Enter the Initial Settings, 3.2-4
3.3
INSTRUMENT VERIFICATION, 3.3-1
Startup the Instrument, 3.3-1
Calibrate the Instrument, 3.3-1
PART B: UPGRADE AND OPTION INSTALLATION
3.4
OPTIONAL TICKET PRINTERS, 3.4-1
Epson TM-290P Slip Printer, 3.4-1
Purpose, 3.4-1
Single-Printer Setup Procedure, 3.4-1
Two-Printer Setup Procedure, 3.4-3
ILLUSTRATIONS
3.1-1
System Electrical Connectors, 3.1-2
3.2-1
3.2-2
3.2-3
Reagent Connections, 3.2-2
System Electrical Connections, 3.2-3
CITIZEN GSX-190 Printer settings, 3.2-4
3.4-1
3.4-2
3.4-3
3.4-4
Epson TM-290P Slip Printer, 3.4-1
DIP Switch, SW1, Location, 3.4-2
Printer Connections for a Single Printer, 3.4-2
Printer Connections for Two Printers, 3.4-4
TABLES
3.1-1
3.1-2
PN 4237242C
Space Requirements, 3.1-1
Connectors’ Part Numbers and Line Input Ranges, 3.1-2
3-i
3
CONTENTS
3-ii
PN 4237242C
C
3INSTALLATION PROCEDURES 3
PART A: INSTRUMENT INSTALLATION
3.1
PREINSTALLATION CHECKS
Space and Accessibility Requirements
If the reagent pack will be placed on the floor, measure the bench height. The reagent pack
should not be more than 36 in. below the lyse pump. This allows for a bench height of about
32 in.
Check the site for proper space allocation (Table 3.1-1)
.
Table 3.1-1 Space Requirements
Linear Dimensions
Required by Instrument
Preferred by Service
Depth
19.23" plus 3" for ventilation
32"
Width
20"
36"
Height
17.9"
32"
Power Requirements
IMPORTANT May compromise instrument results. If you use an extension cord, you could encounter
electrical interference that could affect the instrument’s results. Locate the instrument close enough to a
power outlet that an extension cord is not necessary.
1.
Check for the availability of a power connector.
Make sure the instrument is close enough to a power outlet that the ac power cable safely
reaches it. The ac power cable is 6 ft long and facing the back of the instrument, it is
connected in the lower left corner (Figure 3.1-1).
2.
PN 4237242C
Measure the system power:
r
The MD II Series is labeled and sold for three power ranges, 100 Vac, 120 Vac and
230 Vac.
r
The system can be configured for four different power ranges. Table 3.1-2 shows the
four power ranges and a part number for the associated configuration jumper.
3.1-1
C
INSTALLATION PROCEDURES
PREINSTALLATION CHECKS
Table 3.1-2 Connectors’ Part Numbers and Line Input Ranges
Connector
Range
Part Number
100 VOLTS
90 - 110 Vac
6028623-0
120 VOLTS
110 - 132 Vac
6028600-1
220 VOLTS
198 - 242 Vac
6028624-8*
240 VOLTS
220 - 264 Vac
6028625-6*
* An assembly, PN 6706318-0, is included with the 230 Vac instrument. It has both the 200 VOLTS and
240 VOLTS connectors.
Figure 3.1-1 System Electrical Connectors
POWER LINE
CORD (AC DILUTER
TO ANALYZER)
SERIAL
PORT 2
SERIAL
PORT 1
25-PIN PARALLEL
CONNECTOR
CENTRONICS
PARALLEL
PRINTER
CABLE
PRINTER
MD II
AC
POWER
CABLE
TO POWER
SOURCE
3.1-2
7242035B
PN 4237242C
C
INSTALLATION PROCEDURES
INITIAL SETUP
3.2
INITIAL SETUP
Unpack the Instrument
1.
Unpack all boxes.
2.
Using the packing list, ensure that no items are missing including the proper reagents,
controls and calibrators.
3.
Check the instrument for damage.
4.
Check that the instrument has caution and compliance labels near the power connector.
5.
Measure the line voltage that the MD II will be plugged into. Verify that the instrument
version (100 Vac, 120 Vac or 230 Vac) matches this line voltage. (The range and part
number are stamped on the serial number tag and also listed in Table 3.1-2.)
6.
Remove packing materials. Open the lower chassis as necessary:
r
Wire wrap around the vacuum pump
r
Foam insert underneath the vacuum pump
7.
Ensure that cards and connectors are seated properly in the lower chassis.
8.
Verify that the AC Power/Vacuum Relay card is configured for the proper voltage range.
(Use Table 3.1-2 to match the proper range to the connector/jumper label.) The 230
VOLTS instruments ship with the higher-range connector (240 VOLTS) installed and
also include the lower-range connector, should the input supply be 220 Vac or lower.
Change to the 200 VOLTS jumper at this time if appropriate.
9.
Lower the upper chassis and remove the upper chassis cover.
10. Check that all cards are seated properly, no connectors are loose, and all the DIP switches
and jumpers are set correctly. Refer to Appendix A for the correct settings, if necessary.
11. Verify that the AT power supply Ac Select switch is set to 115 for 100 Vac or 120 Vac
units and 230 for 230 VOLTS units. This switch is covered by the chassis rear panel and
can just be seen through the power cable cutout. To switch voltage settings, you must
remove the power supply.
12. Connect cables and power cords (Figure 3.1-1).
CAUTION Risk of damage to equipment. Turning the instrument’s power ON before the instrument is
completely setup could damage the instrument. Do not turn the instrument’s power ON until you have
completed connecting the reagents and the Printer.
13. Install the peristaltic pump tubing around the pump spooler.
Note: Manually turn the pump several revolutions. If this is not done, the motor may not
be able to turn the pump.
PN 4237242C
3.2-1
3
C
INSTALLATION PROCEDURES
INITIAL SETUP
Connect the Reagents
The connections for the reagents are at the bottom right corner on the left side of the
instrument (Figure 3.2-1).
1.
Connect the diluent reagent using blue stripe tubing (PN 3202209-0) to the blue fitting
(diluent). Use a Luer fitting (PN 6232503-8).
2.
Connect the lytic reagent using EVA tubing (PN 3202221-9) to the white fitting (lyse).
Use a Luer fitting (PN 6232503-8). Use polyurethane tubing (PN 3202036-4) to couple
the EVA tubing to the Luer fitting and to the pickup tube.
3.
Ensure that the lyse container is no higher than 6 in. above or lower than 36 in. below
the lyse pump and that the lyse tubing is no longer than 5 ft.
4.
Connect the waste container using red stripe tubing (PN 3202205-7) to the red fitting
(waste). Use a Luer fitting (PN 6232503-8).
5.
If a cubitainer is used for waste, connect the waste alarm cable (PN 6028669-8) between
the waste pickup tube and the waste sensor connector. The waste sensor connector is
located to the left of the white fitting (lyse).
Figure 3.2-1 Reagent Connections
LYTIC
REAGENT II
CONNECTION
(WHITE)
DILUENT
REAGENT I
CONNECTION
(BLUE)
WASTE
CONNECTION
(RED)
WASTE
SENSOR
CONNECTION
7242016A
3.2-2
PN 4237242C
C
INSTALLATION PROCEDURES
INITIAL SETUP
Connect the Printer
1.
2.
Determine which type of Printer(s) need to be installed.
r
To install the standard Graphic Printer only, follow this procedure.
r
To install the optional Ticket Printer or the optional Ticket Printer and the Graphic
Printer, go to Heading 3.4, OPTIONAL TICKET PRINTERS.
Connect the Printer cable between the Printer and the 25-pin parallel connector at the
back of the MD II (Figure 3.2-2.).
Figure 3.2-2 System Electrical Connections
POWER LINE
CORD (AC DILUTER
TO ANALYZER)
SERIAL
PORT 2
SERIAL
PORT 1
25-PIN PARALLEL
CONNECTOR
CENTRONICS
PARALLEL
PRINTER
CABLE
PRINTER
MD II
AC
POWER
CABLE
TO POWER
SOURCE
3.
Load paper into the Printer.
4.
Set the Printer configuration for MD II use.
7242035B
ATTENTION: The procedures for setting the configuration and the sequence of setting the
configuration and powering up the Printer depends upon the specific Printer. Refer to the
Printer manual for instructions on how to set the Printer’s configuration.
The Printer must be an Epson-compatible Printer, but should be set for IBM® emulation since
some characters are used from the IBM character set. Paper size must be set to 8.5 in. by
11 in. Other settings or options can be set to the customers’ preference. If the site is using the
CITIZEN GSX-190 printer, use the configuration settings in Figure 3.2-3.
5.
PN 4237242C
If you have not plugged the Printer into an available power source and turned the Printer
on, do so now.
3.2-3
3
C
INSTALLATION PROCEDURES
INITIAL SETUP
Figure 3.2-3 CITIZEN GSX-190 Printer settings
Enter the Initial Settings
3.2-4
1.
Insert a Program Disk in the floppy disk drive and turn the instrument’s power ON.
2.
Ensure that the system reaches the Main Menu without detecting any errors.
3.
Set all relevant system settings: date, time, print options, host options and reagent
information.
4.
Set the customer's IQAP # if it is available.
PN 4237242C
C
INSTALLATION PROCEDURES
INSTRUMENT VERIFICATION
3.3
INSTRUMENT VERIFICATION
Startup the Instrument
1.
Prime the reagent and sweep-flow lines using the Cycle Reagents key.
2.
Perform the Hgb Preamp and the Vacuum Adjustment procedures (Headings 4.29 and
4.30, respectively).
3.
Select 3 STARTUP and verify proper backgrounds.
4.
Perform the Latex Gain Adjustment procedure (Heading 4.27).
5.
Set the AIM “target values” (Heading 4.28).
6.
Run controls and verify proper system operation. When preparing for calibration, if the
control results are not within the stated expectations, adjust the calibration factors to the
normal control.
Calibrate the Instrument
Preferably, the trainer performs calibration with the customer as part of customer training. If
you (the installer) are also the trainer, you should perform calibration at this time and include
the customer.
Calibration must follow the procedure outlined in the Calibration section of the Operator’s
Guide.
PN 4237242C
3.3-1
3
C
INSTALLATION PROCEDURES
INSTRUMENT VERIFICATION
3.3-2
PN 4237242C
C
INSTALLATION PROCEDURES
OPTIONAL TICKET PRINTERS
PART B: UPGRADE AND OPTION INSTALLATION
3.4
OPTIONAL TICKET PRINTERS
Epson TM-290P Slip Printer
Purpose
A parallel version of the Epson TM-290P Slip Printer is available for use on the MD II. See
Figure 3.4-1.
Figure 3.4-1 Epson TM-290P Slip Printer
POWER
RELEASE
PAPER OUT
RESET
FORWARD
REVERSE
RELEASE
7303007A
This section contains two procedures. One explains how to install this Ticket Printer instead
of a Graphic Printer (Single-Printer Setup) and the other explains how to install this Ticket
Printer in conjunction with a Graphic Printer (Two-Printer Setup).
For information on how to use this Printer, see the COULTER MD II Series Analyzer Ticket
Printer User’s Guide, PN 4237303. For Printer specifications, DIP switch settings and the
Printer self-test procedure, see Appendix D.
Single-Printer Setup Procedure
1.
2.
PN 4237242C
Unpack the Ticket Printer. Ensure you have the following components:
r
Printer
r
24-V printer power supply
r
Line cord for the power supply
r
Standard Centronics® data cable
r
Ticket Key Disk
r
Ribbon ink cassette.
Locate the DIP switch, SW1, on the bottom of the Printer sled as shown in Figure 3.4-2.
Ensure switch positions 2, 3, and 4 are ON and the rest are OFF. See Table D.1-1 and
Table D.1-2 for the switch position functions.
3.4-1
3
INSTALLATION PROCEDURES
OPTIONAL TICKET PRINTERS
Figure 3.4-2 DIP Switch, SW1, Location
SW 1
ON
1 2 3 4 5 6 7 8 9 10
7303003A
CAUTION Risk of damage to equipment. Turning the instrument’s power ON before connecting the Printer
to the instrument could damage the instrument. Ensure the power is OFF before connecting the Printer.
3.
Ensure the MD II power is off.
4.
Connect the Printer as shown in Figure 3.4-3. Connect the power cord last.
Figure 3.4-3 Printer Connections for a Single Printer
POWER LINE
CORD (AC DILUTER
TO ANALYZER)
SERIAL
PORT 2
SERIAL
PORT 1
25-PIN PARALLEL
CONNECTOR
CENTRONICS
PARALLEL
PRINTER
CABLE
POWER
SUPPLY
TICKET
PRINTER
5.
3.4-2
AC
POWER
CABLE
MD II
7242053B
Perform a Printer self-test on the Ticket Printer as instructed under Heading D.1, EPSON
TM-290P SLIP PRINTER, and verify that all the characters print legibly.
PN 4237242C
INSTALLATION PROCEDURES
OPTIONAL TICKET PRINTERS
6.
Turn on the MD II and print the calibration factors to verify that the Printer and
instrument are working together.
Note: The first time the Ticket Printer option is enabled, the instrument will request that
you insert the Ticket Key Disk. Once the ticket printer enable is recorded in CMOS, it
will not be requested again.
Two-Printer Setup Procedure
1.
Unpack the Ticket Printer. Ensure you have the following components:
r
Printer
r
24-V printer power supply
r
Line cord for the power supply
r
Standard Centronics data cable
r
Ticket Key Disk
r
Ribbon ink cassette.
2.
Locate the DIP switch, SW1, on the bottom of the Printer sled as shown in Figure 3.4-2.
Ensure switch positions 2, 3, and 4 are ON and the rest are OFF. See Table D.1-1 and
Table D.1-2 for the switch position functions for DIP switches SW1 and SW2.
3.
Unpack the optional A/B Switch Box. Ensure the A/B Switch Box kit includes:
r
A/B Switch Box
r
6-ft Centronics cable
r
Installation instructions.
CAUTION Risk of damage to equipment. Turning the instrument’s power ON before connecting the Printer
to the instrument could damage the instrument. Ensure the power is OFF before connecting the Printer.
4.
Ensure the MD II power is off.
5.
Using Figure 3.4-4 as a guide, connect:
6.
PN 4237242C
a.
Graphic Printer to connector A
b.
Ticket Printer to connector B
c.
6-ft Centronics cable between the I/O connector on the A/B Switch Box and the
MD II parallel (Printer) connector
d.
Power cord.
Perform a Printer Self-Test on the Ticket Printer as instructed under Heading D.1,
EPSON TM-290P SLIP PRINTER, and verify that all the characters print legibly.
3.4-3
3
INSTALLATION PROCEDURES
OPTIONAL TICKET PRINTERS
Figure 3.4-4 Printer Connections for Two Printers
POWER LINE
CORD (AC DILUTER
TO ANALYZER)
SERIAL
PORT 2
SERIAL
PORT 1
25-PIN PARALLEL
CONNECTOR
CENTRONICS
PARALLEL
PRINTER
CABLE
A
I/O
A/B
SWITCH
BOX
B
MD II
AC
POWER
CABLE
POWER
SUPPLY
GRAPHIC
PRINTER
7.
8.
TICKET
PRINTER
7242052B
If this is the initial installation of the Graphic Printer:
a.
Load paper into the Graphic Printer.
b.
Set the Printer’s configuration for MD II use. Refer to step 3 of the Connect the
Printer the Printer procedure under Heading 3.2, INITIAL SETUP.
Turn on the MD II and print the calibration factors from both Printers to verify that the
Printers and instrument are working together. Since no menu item will set up both
Printers at once, use the Printers one at a time as follows:
r
r
For the Graphic Printer t
Select 1 Full Page Report or 2 Split Page Report on the Report Format menu
t
Set the A/B Switch Box to A.
For the Ticket Printer t
Select 3 Ticket Report on the Report Format menu
t
Set the A/B Switch Box to B.
Note: The first time the Ticket Printer option is enabled, the instrument will request
that you insert the Ticket Key Disk. Once the Ticket Printer enable is recorded in
CMOS, it will not be requested again.
3.4-4
PN 4237242C
C
CONTENTS
4
SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1
4.2
GUIDELINES, 4.1-1
ACCESSING THE HIDDEN SERVICE MENU ITEMS, 4.2-1
Accessing Service Report, 4.2-1
Purpose, 4.2-1
Procedure, 4.2-1
Accessing Service Diagnostic, 4.2-1
Purpose, 4.2-1
Procedure, 4.2-1
System Reboot, 4.2-2
4.3
REMOVING THE TOP COVER, 4.3-1
4.4
OPENING THE LOWER CHASSIS, 4.4-1
4.5
AT POWER SUPPLY, 4.5-1
Tools/Supplies Needed, 4.5-1
Removal, 4.5-1
Installation, 4.5-1
Verification, 4.5-2
4.6
AT MOTHERBOARD, 4.6-1
Tools/Supplies Needed, 4.6-1
Removal, 4.6-1
Installation, 4.6-2
Verification, 4.6-3
4.7
SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD, 4.7-1
Tools/Supplies Needed, 4.7-1
Removal, 4.7-1
Installation, 4.7-1
Verification, 4.7-2
4.8
USER RESOURCE ADAPTER (URA) CARD, 4.8-1
Tools/Supplies Needed, 4.8-1
Removal, 4.8-1
Installation, 4.8-2
Verification, 4.8-2
4.9
DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS, 4.9-1
Tools/Supplies Needed, 4.9-1
Removal, 4.9-1
Installation, 4.9-2
Verification, 4.9-2
4.10 MEMBRANE KEYPAD AND DISPLAY, 4.10-1
Tools/Supplies Needed, 4.10-1
Removal, 4.10-1
Installation, 4.10-2
Verification, 4.10-2
PN 4237242C
4-i
4
CONTENTS
4.11 AC POWER/VACUUM RELAY CARD, 4.11-1
Tools/Supplies Needed, 4.11-1
Removal, 4.11-1
Installation, 4.11-1
Verification, 4.11-2
4.12 +24 VOLT SWITCHING POWER SUPPLY, 4.12-1
Tools/Supplies Needed, 4.12-1
Removal, 4.12-1
Installation, 4.12-2
Verification, 4.12-3
4.13 LINEAR POWER SUPPLY CARD, 4.13-1
Tools/Supplies Needed, 4.13-1
Removal, 4.13-1
Installation, 4.13-2
Verification, 4.13-3
4.14
VACUUM SENSOR CARD, 4.14-1
Tools/Supplies Needed, 4.14-1
Removal, 4.14-1
Installation, 4.14-2
Verification, 4.14-2
4.15
MOTOR/SOLENOID DRIVER CARD, 4.15-1
Tools/Supplies Needed, 4.15-1
Removal, 4.15-1
Installation, 4.15-1
Verification, 4.15-2
4.16 SENSOR PREAMP ADAPTER (SPA) CARD, 4.16-1
Tools/Supplies Needed, 4.16-1
Removal, 4.16-1
Installation, 4.16-2
Verification, 4.16-3
4.17 PROBE AND PROBE WIPE, 4.17-1
Tools/Supplies Needed, 4.17-1
Removal, 4.17-1
Installation, 4.17-2
Verification, 4.17-3
4.18 PROBE MOTOR, 4.18-1
Tools/Supplies Needed, 4.18-1
Removal, 4.18-1
Installation, 4.18-1
Verification, 4.18-1
4-ii
PN 4237242C
CONTENTS
4.19 TRAVERSE DRIVE BELT, 4.19-1
Tools/Supplies Needed, 4.19-1
Removal, 4.19-1
Installation, 4.19-2
Verification, 4.19-3
4.20 TRAVERSE MOTOR, 4.20-1
Tools/Supplies Needed, 4.20-1
Removal, 4.20-1
Installation, 4.20-2
Verification, 4.20-3
4.21 SYRINGE ASSEMBLY, 4.21-1
Tools/Supplies Needed, 4.21-1
Removal, 4.21-1
Installation, 4.21-3
Verification, 4.21-4
4.22 PERISTALTIC PUMP MOTOR AND SPOOL, 4.22-1
Tools/Supplies Needed, 4.22-1
Removal, 4.22-1
Installation, 4.22-2
Verification, 4.22-2
4.23 VACUUM PUMP, 4.23-1
Tools/Supplies Needed, 4.23-1
Removal, 4.23-1
Installation, 4.23-1
Verification, 4.23-1
4.24 APERTURE, BATH AND APERTURE ELECTRODE MODULE, 4.24-1
Tools/Supplies Needed, 4.24-1
Removal, 4.24-1
Installation, 4.24-2
Verification, 4.24-3
4.25 DILUTER PANEL SOLENOIDS, 4.25-1
Tools/Supplies Needed, 4.25-1
Removal, 4.25-1
Installation, 4.25-1
Verification, 4.25-1
4.26 SWEEP-FLOW TUBING, 4.26-1
Tools/Supplies Needed, 4.26-1
Removal, 4.26-1
Installation, 4.26-2
Verification, 4.26-2
4.27 LATEX GAIN ADJUSTMENT, 4.27-1
Tools/Supplies Needed, 4.27-1
Procedure, 4.27-1
PN 4237242C
4-iii
4
CONTENTS
4.28 AIM ADJUSTMENT, 4.28-1
Tools/Supplies Needed, 4.28-1
Procedure, 4.28-1
Optional Procedures, 4.28-1
Option 1, 4.28-1
Option 2, 4.28-2
4.29 HGB PREAMP ADJUSTMENT, 4.29-1
Tools/Supplies Needed, 4.29-1
Procedure, 4.29-1
4.30 VACUUM ADJUSTMENT, 4.30-1
Procedure, 4.30-1
4.31 LYSE VOLUME ADJUSTMENT/VERIFICATION, 4.31-1
Tools/Supplies Needed, 4.31-1
Procedure, 4.31-1
ILLUSTRATIONS
4.3-1
Top View into Upper Chassis, 4.3-1
4.4-1
Top View into Lower Chassis, 4.4-1
4.6-1
4.6-2
Card Removal (AT Motherboard, URA, DRAs and SPAD), 4.6-1
AT Motherboard, 4.6-3
4.8-1
URA Card, 4.8-1
4.9-1
DRA Card, 4.9-1
4.10-1 Membrane Keypad and Display, 4.10-1
4.12-1 +24 V Power Supply, 4.12-1
4.13-1 Linear Power Supply Card, 4.13-1
4.13-2 Back View of MD II with Rear Panel Door Opened, 4.13-2
4.14-1 Vacuum Sensor Card, 4.14-1
4.15-1 Motor/Solenoid Driver Card, 4.15-2
4.16-1 Sensor Preamp Adapter (SPA) Card and Surrounding Components, 4.16-1
4.16-2 Sensor Preamp Adapter Card, 4.16-3
4.17-1 Probe Assembly, 4.17-1
4.19-1 Traverse Drive Belt, 4.19-1
4.20-1 Traverse Motor, 4.20-1
4-iv
PN 4237242C
CONTENTS
4.21-1 Syringe Assembly, 4.21-2
4.22-1 Peristaltic Pump Motors, 4.22-1
4.24-1 Aperture Bath Assembly, 4.24-1
4.26-1 Sweep-Flow Tubing, 4.26-1
PN 4237242C
4-v
4
CONTENTS
4-vi
PN 4237242C
C
4SERVICE AND REPAIR PROCEDURES 4
4.1
GUIDELINES
PN 4237242C
r
All the tools and supplies you need to perform a specific procedure are listed under
Tools/Supplies Needed, at the beginning of the applicable procedure.
r
Each time you are told to turn the instrument’s power ON, you should have the Program
Disk in the floppy disk drive.
r
If you are told to let the system reach the Main Menu and it does not, or you are told to
perform a System Verification Procedure (SVP) and the system fails, you will have to
troubleshoot the problem. Refer to Chapter 7, Troubleshooting, for guidelines.
r
Verify that each connector has a label. Tracing where each connector goes when
installing the new card would be very time consuming without labels.
4.1-1
SERVICE AND REPAIR PROCEDURES
GUIDELINES
4.1-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
ACCESSING THE HIDDEN SERVICE MENU ITEMS
4.2
ACCESSING THE HIDDEN SERVICE MENU ITEMS
There are three service functions hidden (they do not show up on the display) from the
customer. They are:
r
Service Report
r
Service Diagnostic
r
System Reboot.
Accessing Service Report
Purpose
Access the Service Report to obtain data about the previous aspirate cycle, whether it was a
sample, a control or a calibrator. The Service Report data does not appear on any customer
screen or printout. For detailed information on the contents of the Service Report, see
Heading 7.2, GENERATING A SERVICE REPORT.
Procedure
To access the Service Report, at the Main Menu select 5 SPECIAL FUNCTIONS tt 4 SUPERVISOR tt 7
[no menu item displays on the screen].
A partial report with Aperture Integrity Monitor (AIM) data displays on the screen but does
not print unless you have the Auto Print function active. If the Auto Print function is active, a
more comprehensive report is automatically sent to the printer.
Accessing Service Diagnostic
Purpose
A Service Diagnostic diluter table (or cycle) is included in the instrument. The table energizes
solenoids and motors, one by one. The Service Diagnostic diluter table (Table 7.3-1) is shown
in Heading 7.3, SERVICE DIAGNOSTIC.
Procedure
Once you begin the Service Diagnostic, you must advance completely through the table.
Pressing Escape will not exit the cycle.
1.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 7 [no menu item
displays on the screen].
2.
When the instrument prompts you for a password, type 123.
After entering the password, solenoid LV1, the vacuum transducer vent valve located on
the Vacuum Sensor card is energized and the cycle begins.
PN 4237242C
4.2-1
4
SERVICE AND REPAIR PROCEDURES
ACCESSING THE HIDDEN SERVICE MENU ITEMS
System Reboot
4.2-2
1.
Insert the Program Disk.
2.
From the Main Menu, select 7 [no menu item displays on the screen].
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
REMOVING THE TOP COVER
4.3
REMOVING THE TOP COVER
1.
At the rear of the unit, unscrew the three, #2 Phillips-head screws, Figure 4.3-1.
2.
Slide the cover off toward the front of the instrument.
Figure 4.3-1 Top View into Upper Chassis
AT
POWER SUPPLY
SCREWS
COVER
SCREWS
AC POWER CABLE
DRA
CARDS
SPAD
CARD
J15 SER1
AT
POWER SUPPLY
P8
J17 FLOPPY
J18 PARALLEL
J14 SER2
URA
CARD
AT
MOTHERBOARD
P9
SW1
J20 IDE
J19
EXT BATT
FLOPPY
DISK
DRIVE
BATTERY
BANK 0
BANK 1
BANK 2
BANK 3
W3
7
4
1
_
MEMBRANE
KEYPAD/DISPLAY
PN 4237242C
8
5
2
0
9
6
3
.
7242018A
4.3-1
4
SERVICE AND REPAIR PROCEDURES
REMOVING THE TOP COVER
4.3-2
PN 4237242C
A
SERVICE AND REPAIR PROCEDURES
OPENING THE LOWER CHASSIS
4.4
OPENING THE LOWER CHASSIS
1.
Open the front door of the instrument.
2.
Loosen the captive #2 Phillips-head retaining screw just above the traverse motor
(Figure 4.4-1).
3.
Swing the upper chassis up and back and lock the support arms in place.
Figure 4.4-1 Top View into Lower Chassis
90° POSITION
45° POSITION
RETAINING
SCREW
+24 V
POWER SUPPLY
MOTOR/SOLENOID
DRIVER CARD
LINEAR
POWER
SUPPLY CARD
AC POWER/
VACUUM RELAY
CARD
-V4 +
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
A
C P
O
W
E
R
/V
A
C
U
U
M
V1
+
RTN RTN
+
FLEX
CONNECT
CARD
VACUUM
SENSOR
CARD
MOTOR/SOLENOID DRIVER CARD
VACUUM
PUMP
SENSOR
PREAMP
ADAPTER
CARD
SOLENOID
INTERCONNECT
CARD
PN 4237242A
HGB PREAMP
CARD
7242019A
4.4-1
4
SERVICE AND REPAIR PROCEDURES
OPENING THE LOWER CHASSIS
4.4-2
PN 4237242A
C
SERVICE AND REPAIR PROCEDURES
AT POWER SUPPLY
4.5
AT POWER SUPPLY
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B Voltmeter
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable from the rear of the power supply (Figure 4.3-1).
3.
Remove the top cover as directed under Heading 4.3.
4.
Remove electrical connections to the power supply:
r
P1 connects to the floppy disk drive power connector.
r
P2 connects to a cable from the lower chassis terminating at the AC Power/Vacuum
Relay card.
r
P4 and P5 connect to the AT motherboard connectors P8 and P9.
5.
At the rear of the instrument, remove the two #2 Phillips-head screws holding the power
supply to the chassis (Figure 4.3-1).
6.
Remove the power supply from the chassis.
Installation
1.
Ensure that the input voltage (115 V or 230 V) is set correctly on the new power supply.
Use the slide switch just above the ac line connector to set the input voltage.
2.
Install the new power supply. The power supply "hooks" into the chassis and must be
slid from front to back to properly engage, before fastening screws.
3.
At the rear of the instrument, replace the two #2 Phillips-head screws holding the power
supply to the chassis (Figure 4.3-1).
CAUTION Risk of electronic damage. If connectors P4 and P5 are attached incorrectly, the motherboard will
be damaged. They can only be installed correctly one way. They are, however, identical and could easily be
switched. To ensure that P4 and P5 are installed correctly, attach them so that the black wires from each
connector are situated together in the middle.
PN 4237242C
4.5-1
4
SERVICE AND REPAIR PROCEDURES
AT POWER SUPPLY
4.
5.
Connect the power supply to the instrument:
r
P1 connects to the floppy disk drive power connector.
r
P2 connects to a cable from the lower chassis terminating at the AC Power/Vacuum
Relay card.
r
P4 and P5 attach to the AT motherboard connectors P8 and P9.
Reconnect the ac power cable and turn ON the instrument’s power. Wait until the
instrument reaches the Main Menu. It is difficult to check power before connecting to
the motherboard, since the power supply requires a load before it turns on any supplies,
including the power for the fan.
Verification
1.
4.5-2
Check voltages at any connector. A label showing a detailed pinout is affixed to the top
of the AT power supply and greater detail is also available from the System Interconnect
diagram (Chapter 6, Schematics and Block Diagrams). Even without a voltmeter, if the
system reaches the Main Menu after power ON, the +5, +12, and -12 V supplies must be
present.
r
Red wires are +5 V
r
Yellow wires are +12 V
r
Orange wires are -5 V
r
Blue wires are -12 V.
2.
Replace the top cover.
3.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
AT MOTHERBOARD
4.6
AT MOTHERBOARD
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable from the rear of the power supply (Figure 4.3-1).
3.
Remove the top cover as directed under Heading 4.3.
4.
Remove the SPAD card (Heading 4.7), the URA card (Heading 4.8) and the two DRA
cards (Heading 4.9) shown in Figure 4.6-1.
5.
Disconnect P4 and P5 of the AT power supply from P8 and P9 on the AT motherboard.
Figure 4.6-1 Card Removal (AT Motherboard, URA, DRAs and SPAD)
MOUNTING
BRACKET
AT
MOTHERBOARD
SIMM
MEMORY
NYLON
RETAINING
POST
(8)
PN 4237242C
GROUND
SCREW
(2)
7242037A
4.6-1
4
SERVICE AND REPAIR PROCEDURES
AT MOTHERBOARD
6.
Disconnect the floppy disk drive, the external interface cables and the speaker:
r
Floppy disk drive connects to J17.
r
Serial 1 port connects to J15.
r
Serial 2 port connects to J14.
r
Parallel (Printer) port connects to J18.
r
Speaker port connects to J22.
7.
Remove the two motherboard ground screws (Figure 4.6-2).
8.
Remove the nylon retaining posts (Figure 4.6-1):
a.
Start at one end and work to the other.
b.
Squeeze the nylon retaining posts while lifting the AT motherboard, until the card
can be completely removed.
Installation
1.
Press the new motherboard onto the nylon retaining posts and attach the two ground
screws.
2.
Verify the proper switch and jumper settings (Figure 4.6-2).
3.
Verify that the SIMM memory is in place and properly seated (Figure 4.6-1).
4.
Connect the external interface cables, the speaker and the floppy disk drive
(Figure 4.6-2):
r
Serial 1 port connects to J15.
r
Serial 2 port connects to J14.
r
Parallel (Printer) port connects to J18.
r
Speaker port connects to J22.
r
Floppy disk drive connects to J17.
CAUTION Risk of electronic damage. If connectors P4 and P5 are attached incorrectly, the motherboard will
be damaged. They can only be installed correctly one way. They are, however, identical and could easily be
switched. To ensure that P4 and P5 are installed correctly, attach them so that the black wires from each
connector are situated together in the middle.
4.6-2
5.
Connect P4 and P5 of the AT power supply to J8 and J9 on the motherboard.
6.
Install the SPAD (Heading 4.7), URA (Heading 4.8) and both DRA (Heading 4.9) cards.
7.
Replace the top cover.
8.
Reconnect the ac power cable.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
AT MOTHERBOARD
Verification
Without a keyboard and monitor the best way to verify AT motherboard operation is to use
the instrument.
1.
Turn ON the instrument’s power and check to see that the system reaches the Main
Menu. This tells you that you have successfully:
r Passed BIOS POST
r Booted
r Read from the floppy disk drive
r Executed a program
r Communicated with all four cards plugged into the bus.
Figure 4.6-2 AT Motherboard
J15
(Serial 1 port)
J15 SER1
J14
(Serial 2 port)
P8
J18
(Parallel printer
port)
J17 FLOPPY
J18 PARALLEL
J14 SER2
GROUND
SCREW
P9
J17
(Floppy disk
drive)
SW1
J19
EXT BATT
J20 IDE
SW1
BATTERY
CC MD V1.0
NYLON
MOUNTING
POST(8)
BANK 0
BANK 1
BANK 2
BANK 3
W3
W3
(1-2, 3-4)
J21
(Reset)
J22
(Speaker)
PN 4237242C
J23
(Keylock)
J24
(IDE
activity)
J26
(Turbo
SW)
J25
(Turbo
LED)
GROUND
SCREW
7242009A
4.6-3
4
SERVICE AND REPAIR PROCEDURES
AT MOTHERBOARD
4.6-4
2.
Verify the ability to write to the floppy disk drive by temporarily changing a
configuration item, such as a host or Printer option. This forces the system to write the
new configuration to disk. If no problem is observed, restore the system to its original
configuration.
3.
Verify the operation of the Printer port by printing a configuration item such as the
calibration factors.
4.
If the customer uses a host computer, have a sample sent to the host to verify Serial 1
port operation.
5.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD
4.7
SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD
Tools/Supplies Needed
B #1 Phillips-head screwdriver
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Remove the top cover as directed under Heading 4.3.
3.
Locate the SPAD card (Figure 4.3-1).
4.
Remove the screw that fastens the card's metal-mounting bracket to the rear of the
chassis by using a #1 Phillips-head screwdriver (Figure 4.6-1).
5.
Remove all the cables connected to the card (J1 through J4, J8 and J9). Note where each
goes for ease of installation.
6.
Remove the SPAD card.
Installation
PN 4237242C
1.
On the new SPAD card, verify that jumper X7 is ON.
2.
Install the SPAD card into the rightmost (facing the instrument), 16-bit slot
(Figure 4.6-1).
3.
Reconnect the card’s metal-mounting bracket to the rear of the chassis with the
Phillips-head screw (Figure 4.6-1).
4.
Reconnect the cables:
r
J1, J2, J3 take coaxial cables from the Sensor Preamp Adapter card carrying the PLT,
RBC and WBC signals, respectively.
r
J4 (from the Sensor Preamp Adapter card) is a 4-pin connector.
r
J5 is present but is unused at this time.
r
J8 (from the Linear Power Supply card) is a large 10-pin connector.
r
J9 (RBC and WBC AIM voltage to the URA card).
5.
Replace the top cover.
6.
Turn ON the instrument’s power.
4.7-1
4
SERVICE AND REPAIR PROCEDURES
SENSOR PROCESSING ADAPTER WITH DIAGNOSTICS (SPAD) CARD
Verification
4.7-2
1.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 3 PULSE TEST.
2.
Verify that the results match the expected results.
3.
Perform an SVP (Heading 5.1) making sure the histograms display as expected.
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
USER RESOURCE ADAPTER (URA) CARD
4.8
USER RESOURCE ADAPTER (URA) CARD
Tools/Supplies Needed
B #1 Phillips-head screwdriver
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
Remove the top cover as directed under Heading 4.3.
4.
Locate the URA card (Figure 4.3-1).
5.
Remove the screw that fastens the card's metal mounting bracket to the rear of the
chassis by using a #1 Phillips-head screwdriver.
6.
Remove the seven cables connected to the card (J1 through J7).
7.
Remove the URA card.
Figure 4.8-1 URA Card
J5
(KEYBOARD)
R5
(Display contrast)
PN 4237242C
J3
(AIM)
J7
(PREAMP
DIAG)
R6
(A/D zero)
J6
(Hgb)
R7
(A/D adjust)
J2
(PVAC)
J1
(POWER)
CMOS
RAM
SW1
(1-8, OFF)
P2
J4
(Display)
UTILITY TIMER
CIRCUIT
P1
X1
(ON)
7242010A
4.8-1
4
SERVICE AND REPAIR PROCEDURES
USER RESOURCE ADAPTER (URA) CARD
Installation
1.
On the new URA card, Figure 4.8-1, verify that:
r
Jumper X1 is ON
r
All eight positions of switch SW1 are OFF.
2.
Install the URA card into the second, 16-bit expansion slot from the left (facing the
instrument).
3.
Replace the screw that fastens the card's metal mounting bracket to the rear of the
chassis by using a #1 Phillips-head screwdriver (Figure 4.6-1).
4.
Reconnect cables J1 through J7 to the card:
r
J1 - supplies power to the linear supply in the lower chassis.
r
J2 - the vacuum voltage from the Vacuum Sensor card.
r
J3 - the AIM voltage from the SPAD card.
r
J4 - a ribbon cable going to the LCD screen.
r
J5 - a flex cable coming from the keypad.
r
J6 - Hgb preamp.
r
J7 - the preamp diagnostics voltages.
5.
Replace the top cover.
6.
Reconnect the ac power cable.
Verification
There are five functions of the URA card that need to be verified. The two most important
functions, interfacing the display and the keypad, can be checked by running a cycle. Going
through various menus will make use of many keys as well.
1.
Turn ON the instrument’s power and allow the system to reach the Main Menu. During
power ON, two voltage checks are made. If no errors display, the A/D converter is
operating properly.
2.
Use various items to check the display and keypad. For example, verify that the display
and keypad are operating properly by inputting a 10-digit Sample ID at the Main Menu.
3.
Go through all the screens in the Setup menu and make sure that they match the
customers settings. From the Main Menu, select 5 SPECIAL FUNCTIONS tt 1 SETUP.
Note: There is CMOS RAM (Figure 4.8-1) on the URA card containing all the customer’s
configuration settings (Printer options, control options, and host settings). If the new
URA CMOS memory is clear, and the system booted with the same disk that the
customer was using, the settings will match. Any other set of circumstances will produce
different settings. For more information on what could happen with these settings, see
the description of the URA card in Heading 2.4.
4.8-2
4.
With a sample running, verify that the “running marker” in the upper-right corner of the
display is “rotating.” This makes use of the Utility Timer circuit on the URA card.
5.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS
4.9
DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS
Tools/Supplies Needed
B #1 Phillips-head screwdriver
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Remove the top cover as directed under Heading 4.3.
3.
Locate the DRA card(s) you are replacing (Figure 4.6-1).
4.
Remove the screw that fastens the card's metal mounting bracket to the rear of the
chassis by using a #1 Phillips-head screwdriver (Figure 4.6-1).
Figure 4.9-1 DRA Card
TO
MOTOR/SOLENOID
DRIVER CARD
J3
J2
X5
(Sets card
for #1 or #2)
X4
(Sets IRQ
for 11 or 12)
X6
(ON)
J1
7242011A
PN 4237242C
5.
Remove the three ribbon cables (P1, P2, P3) connected to the Motor/Solenoid Driver
card and the AIM voltage cable connecting the SPAD card to the URA card.
6.
Remove the DRA card(s).
4.9-1
4
SERVICE AND REPAIR PROCEDURES
DILUTER RESOURCE ADAPTER (DRA) 1 AND 2 CARDS
Installation
1.
On the new DRA card(s), verify all the jumper settings (Figure 4.9-1):
r
X6 - use for both DRA cards.
r
DRA1 - X5 ON and X4 jumpered for IRQ11.
r
DRA2 - X5 OFF and X4 jumpered for IRQ12.
2.
Install DRA card(s). DRA1 goes into the fourth, 16-bit expansion slot from the left
(facing the instrument) and DRA2 goes into the third, 16-bit expansion slot from the left
(Figure 4.6-1).
3.
Replace the screw that fastens the card's metal mounting bracket to the rear of the
chassis by using a #1 Phillips-head screwdriver (Figure 4.6-1).
4.
Reconnect the three ribbon cables (P1, P2, P3) to the Motor/Solenoid Driver card and
the AIM voltage cable connecting the SPAD card to the URA card.
5.
Replace the top cover.
Verification
1.
Turn ON the instrument’s power.
2.
Observe that both syringes operate while the system is powering up.
3.
Verify the operation of all the solenoid valves and the Traverse Assembly mechanism by
using the Service Diagnostic as directed under Heading 4.2.
4.
Verify the operation of the peristaltic pumps:
5.
4.9-2
a.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS.
b.
Select 4 RINSE to check PM3.
c.
Select 3 DRAIN to check PM4.
d.
Select 5 MIX to check PM1.
e.
Select 4 RINSE several times, until reservoir must refill, to check PM2.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
MEMBRANE KEYPAD AND DISPLAY
4.10
MEMBRANE KEYPAD AND DISPLAY
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B 1/4 in. nut driver
Removal
1.
Turn OFF the instrument’s power.
2.
Remove the top cover as directed under Heading 4.3.
3.
Disconnect the following cables (Figure 4.10-1):
4.
a.
The display cable (ribbon) from J4 on the URA card.
b.
The keypad cable (flex) from J5 on the URA card.
c.
The keypad flex cable shield ground from its connection post on the chassis.
Remove the eight, #2 Phillips-head screws that fasten the keypad/display assembly to the
chassis (Figure 4.10-1).
Figure 4.10-1 Membrane Keypad and Display
DISPLAY
CABLE
KEYPAD
CABLE
FLEX CABLE
SHIELD
GROUND
URA
CARD
KEYPAD
PHILLIPSHEAD
SCREW
(8)
DISPLAY
7242038A
5.
PN 4237242C
Remove the keypad/display assembly from the chassis, being careful not to snag one of
the connectors as you pull the cables through the chassis opening (Figure 4.10-1).
4.10-1
4
SERVICE AND REPAIR PROCEDURES
MEMBRANE KEYPAD AND DISPLAY
6.
Remove the four KEPS nuts and four flat washers (on the back of the display) that fasten
the display to the keypad.
7.
Remove the display, being careful not to lose any of the four spacers underneath it.
Installation
1.
Fasten the new display and keypad together:
a.
Place the spacer hardware on each threaded post.
b.
Install the display on the posts.
c.
Place a flat washer on each post.
d.
Thread the KEPS nut.
2.
Feed the three cables through the chassis opening, being careful not to snag one of the
connectors as you pull the cables through the chassis opening (Figure 4.10-1).
3.
Fasten the keypad/display assembly to the chassis with the eight, #2 Phillips-head
screws.
4.
Reconnect the cables (Figure 4.10-1):
a.
The display cable (ribbon) to J4 on the URA card.
b.
The keypad cable (flex) to J5 on the URA card.
c.
The keypad flex cable shield ground to its connection post on the chassis.
Note: Make sure that these cables do not interfere with the travel of the traverse rack.
5.
Replace the top cover and turn ON the instrument’s power.
Verification
4.10-2
1.
Navigate through the instrument’s menus, and run a sample cycle to verify the operation
of all the keypad switches.
2.
Observe the display and check for any problems with the visual output, as you check the
operation of the keypad switches.
3.
If no problems are observed, perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
AC POWER/VACUUM RELAY CARD
4.11
AC POWER/VACUUM RELAY CARD
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
WARNING Risk of personal injury. There is a shock hazard if the power cord is not disconnected. Unplug
the ac power cable.
2.
Disconnect the ac power cable.
3.
Open the lower chassis as directed under Heading 4.4 and locate the AC Power/Vacuum
Relay card (Figure 4.4-1).
4.
Disconnect the six wiring connections (P1 through P5 and P7). Several are the same, so
make sure they are properly labeled to facilitate reconnecting them to the replacement
card.
5.
Remove the card by pulling it off the posts. The card is fastened using four snap-on
posts.
Installation
PN 4237242C
1.
Remove the voltage selection connector/jumper P6 from J6 and install it on the
replacement card.
2.
Verify that the on-card fuse is in place and is 1/4 A.
3.
Snap the replacement AC Power/Vacuum Relay card in place.
4.
Connect the six wiring connectors. P1 through P5 and P7 attach to J1 through J5 and J7,
respectively.
5.
Close the lower chassis.
6.
Reconnect the ac power cable.
4.11-1
4
SERVICE AND REPAIR PROCEDURES
AC POWER/VACUUM RELAY CARD
Verification
1.
Turn ON the instrument’s power.
2.
Wait for the system to reach the Main Menu. This verifies the operation of relay K2.
Note: Power tests are performed during the power-up cycle to verify the +24 V power
supply and the Linear Power Supply card voltages. If the tests are passed, the K2 relay is
working properly.
4.11-2
3.
Verify relay K1 operation by either listening for the vacuum pump to turn on during
self-test or doing a Vacuum Adjustment procedure (Heading 4.30) to turn on the
vacuum pump.
4.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
+24 VOLT SWITCHING POWER SUPPLY
4.12
+24 VOLT SWITCHING POWER SUPPLY
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B Large flat-blade screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
At the rear of the instrument, loosen the three captive thumbscrews (Figure 4.12-1) at
the top of the rear panel drop-down door. These thumbscrews are recessed and may
require the use of a large flat-blade screwdriver.
Figure 4.12-1 +24 V Power Supply
FROM
SWITCHING
SUPPLY
REAR VIEW (PANEL DOWN)
THUMBSCREWS(3)
AC INPUT
WIRES
(3)
ACL ACN
COMPUTER POWER
AC POWER
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
-V4 +
V1
+
RTN RTN
+
V3
ADJ
+24 V
POWER SUPPLY
V4
ADJ
MOTOR/SOLENOID
DRIVER CARD
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
-V4 +
V1
RTN RTN +
+
POWER ON SIGNAL
POWERFAIL SIGNAL
OUTPUT LINES
(to J15)
7242020A
PN 4237242C
+24 V RETURN LINES
+24 V
4.
Drop down the rear panel.
5.
Disconnect the three ac input wires from the power supply. They are attached to a
three-position terminal strip (Figure 4.12-1).
4.12-1
4
SERVICE AND REPAIR PROCEDURES
+24 VOLT SWITCHING POWER SUPPLY
6.
7.
Disconnect the output and signal wires (Figure 4.12-1). The new part should include a
harness with this wiring attached.
a.
If the harness is included, open up the lower chassis to gain access to the front of
the Motor/Solenoid Driver card and disconnect P15. Some wire wraps may also have
to be cut to release the harness.
b.
If the harness is not included, disconnect the wires going to the four-position
terminal strip and remove the four-pin connector beside the terminal strip.
1)
Disconnect the three red wires, two from the Motor/Solenoid Driver card and
one from the (+S) on the four-wire connector from the two (+) terminals.
2)
Disconnect the three black wires, two from the Motor/Solenoid Driver card and
one from the (-S) on the four-wire connector from the two (RTN) terminals.
Remove the power supply. The power supply is held on with four, #2 Phillips-head
screws accessed from the backside of the rear panel.
Installation
4.12-2
1.
Attach the new +24 V power supply to the rear panel using the four Phillips-head screws.
2.
Connect the three ac input wires that are attached to a three-position terminal strip
(Figure 4.12-1):
r
The brown line (hot wire) connects to ACL.
r
The blue return line connects to ACN.
r
The green/yellow stripe wire connects to ground.
3.
If the output wiring harness has been provided with the supply, open the lower chassis to
gain access to the front of the Motor/Solenoid Driver card and connect P15 to J15.
4.
If the output wiring harness has not been provided, connect the four-position plug. It
only goes on one way and has the power ON/OFF, powerfail (PF/PG), and output sense
lines.
5.
Connect the output lines to the four-position terminal strip (Figure 4.12-1):
a.
Connect the three red wires, two from the Motor/Solenoid Driver card and one from
the (+S) on the four-wire connector to the two (+) terminals.
b.
Connect the three black wires, two from the Motor/Solenoid Driver card and one
from the (-S) on the four-wire connector to the two (RTN) terminals.
6.
Close the rear panel.
7.
Reconnect the ac power cable.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
+24 VOLT SWITCHING POWER SUPPLY
Verification
1.
Turn ON the instrument’s power and allow the system to reach the Main Menu. This
verifies the operation of the +24 V power supply.
Note: The +24 V turns on when the system software is loaded. An error is displayed on
the screen (ERROR DETECTED (015) Power Supply Failure) if there is no +24 V, and the
Main Menu is never displayed on the screen. Since this power supply has regulating
sense lines, it self-adjusts, checking and adjusting with a voltmeter is unnecessary. If the
power supply is unable to attain +24 V, it signals the instrument that there is a problem
and ERROR DETECTED (015) Power Supply Failure is displayed on the screen.
2.
PN 4237242C
Perform an SVP (Heading 5.1).
4.12-3
4
SERVICE AND REPAIR PROCEDURES
+24 VOLT SWITCHING POWER SUPPLY
4.12-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
LINEAR POWER SUPPLY CARD
4.13
LINEAR POWER SUPPLY CARD
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B Large flat-blade screwdriver
B Voltmeter
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
At the rear of the instrument, loosen the three captive thumbscrews at the top of the rear
panel drop-down door. These thumbscrews are recessed and may require the use of a
large flat-blade screwdriver (Figure 4.12-1).
4.
Drop down the rear panel.
5.
Electrically disconnect the Linear Power Supply card from the system. There are five
connectors (Figure 4.13-1) to remove from the card (J1 through J5).
Figure 4.13-1 Linear Power Supply Card
J5
(RBC and WBC
aperture voltage,
zap voltage out)
J3
(Commands,
+5 V in from
SPAD card)
J2
(Hgb LED
current
supply)
X3
(OFF)
X1
(OFF)
X2
(OFF)
J4
(+15 Vdc
out)
J1
(Transformer
ac input to Linear
Power Supply card)
TP11
(Zap voltage)
TP8
TP10
(WBC aperture
voltage)
TP9
(RBC aperture
voltage)
TP7
TP6
TP5
TP4
TP3
TP2
TP1
7242004A
PN 4237242C
4.13-1
4
SERVICE AND REPAIR PROCEDURES
LINEAR POWER SUPPLY CARD
6.
Remove the card from the rear panel, Figure 4.13-2:
a.
Remove the #2 Phillips-head screw in the center of the card.
b.
Pull the card off its four snap-type mounting posts.
Figure 4.13-2 Back View of MD II with Rear Panel Door Opened
T1
LINEAR POWER
SUPPLY CARD
#2 PHILLIPSHEAD SCREW
J1
TEST
POINTS
JUMPERS
(X1, X2, X3)
7242021A
Installation
4.13-2
1.
On the new Linear Power Supply card, verify that jumpers X1, X2, and X3 are OFF
(Figure 4.13-2).
2.
Install the new Linear Power Supply card, securing it with the #2 Phillips-head screw
(Figure 4.13-2).
3.
Connect P1 (from transformer T1) to J1 (on the Linear Power Supply card), see
Figure 4.13-2.
4.
Reconnect the ac power cable.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
LINEAR POWER SUPPLY CARD
Verification
1.
Turn ON the instrument’s power. The Linear Power Supply card should produce voltages
immediately.
ATTENTION: The Linear Power Supply card is grounded to the Sensor Preamp Adapter card.
Since the Sensor Preamp Adapter card is not yet connected, you must use the associated black
reference test point with each voltage test point to get a proper reading.
2.
Using your voltmeter, verify that you have:
r
+15 Vdc ±0.75 V
r
-15 Vdc ±0.75 V
r
+240 Vdc ±24 V.
3.
Turn OFF the instrument’s power.
4.
Connect the four output connectors. P2 through P5 connect to J2 through J5,
respectively.
5.
Turn ON the instrument’s power.
6.
Measure the Hgb LED current supply by connecting the voltmeter negative lead to TP7
(CATHODE) and the positive lead to TP8 (ANODE), Figure 4.13-1. It should measure
approximately 2.2 Vdc.
Note: This voltage is dependent on other components and does not have a specific stated
range.
7.
Close the rear panel door and replace the three captive thumbscrews.
8.
Verify that the system reached the Main Menu. If it did not, turn the instrument’s power
OFF then back ON again.
9.
Verify that the Hgb voltage is between 3.5 and 4.5 V by performing the Hgb Preamp
Adjustment procedure as directed under Heading 4.29.
10. Perform an SVP (Heading 5.1).
PN 4237242C
4.13-3
4
SERVICE AND REPAIR PROCEDURES
LINEAR POWER SUPPLY CARD
4.13-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
VACUUM SENSOR CARD
4.14
VACUUM SENSOR CARD
Tools/Supplies Needed
B Needle-nose pliers
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
Open up the lower chassis as directed under Heading 4.4 and locate the Vacuum Sensor
card (Figure 4.4-1). It is preferable to open the chassis door a full 90 degrees.
4.
Remove the card by pulling it off the three snap-on posts, Figure 4.14-1, and out of the
lower chassis as far as the wires and tubing will allow. The card snaps onto three snap-on
posts.
Figure 4.14-1 Vacuum Sensor Card
LV1
VACUUM
SENSOR
CARD
LV2
ELECTRICAL
CONNECTIONS
TO FF9-1
TO FF10-1
PRESSURE
TRANSDUCER
PORT (P2)
PN 4237242C
7242039A
4.14-1
4
SERVICE AND REPAIR PROCEDURES
VACUUM SENSOR CARD
5.
Disconnect the three electrical connectors J1, J2, J3.
6.
Remove the two tubes coming from the VIC that attach to solenoids LV1 (from FF 9-1)
of the normally open port and LV2 (from FF 10-1) of the normally closed port
(Figure 4.14-1).
Note: Marking these lines before disconnecting them will save having to trace them back
on installation.
7.
Remove the tubing and adapters connecting pressure transducer port P2 and the
common port of LV1 (Figure 4.14-1).
Installation
1.
Connect the tubing from the common port of LV1 to port P2 of the pressure transducer
(Figure 4.14-1). Use new tubing if replacement looks necessary.
2.
Connect the tubing that traces back to a Y-fitting (FF 9-1), to the normally open port of
LV1 (Figure 4.14-1).
3.
Connect the tubing that eventually traces back to a Y-fitting (FF 10-1), then a fluid
barrier, to the normally closed port of LV2 (Figure 4.14-1).
4.
Connect the electrical connectors (Figure 4.14-1):
r
P1 to J1
r
P2 to J2
r
P3 to J3.
5.
Install the new Vacuum Sensor card onto its three snap-on posts (Figure 4.14-1).
6.
Close the upper chassis.
7.
Reconnect the ac power cable.
8.
Turn ON the instrument’s power.
Verification
4.14-2
1.
Perform the Vacuum Adjustment procedure (Heading 4.30). If no adjustment is needed,
misadjust and readjust to verify that the Vacuum Sensor tracks vacuum changes.
2.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
MOTOR/SOLENOID DRIVER CARD
4.15
MOTOR/SOLENOID DRIVER CARD
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
Open the lower chassis as directed under Heading 4.4 and locate the Motor/Solenoid
Driver card (Figure 4.4-1).
4.
Remove the Phillips-head shipping screw from the card, if it is still present. This screw,
located in the upper left portion of the card just below connector J18 and accessed from
the rear, does not need to be reused once the instrument is installed.
5.
Slide the card partially out of the unit. While easily accessible, remove connectors P7,
P8, P11, and P10.
6.
Slide the card all the way down and remove the remaining connectors (P6, P2, P3, P15,
P18, P4, P23, P5 and P1).
a.
Remove connectors in a top-down manner and lift the card as they are removed.
b.
Verify that each connector is labeled. If not, label at this time.
Note: Tracing where each connector goes when installing the new card would be
very time consuming without labels.
7.
Slide the card up and out, it is not attached.
Installation
CAUTION Risk of electronic damage. Leaving jumper X1 OFF will cause damage to the card and possibly
other components. The damage may not happen right away, but could happen during any instrument power
ON. Before proceeding, make sure Jumper X1 is ON. See Figure 4.15-1.
PN 4237242C
1.
Inspect the new card, making sure that jumpers X1 and X2 are ON (Figure 4.15-1).
2.
Slide the card partially into the unit. While still easily accessible, attach connectors P7,
P8, P11, and P10.
3.
Slide the card all the way down and attach the remaining connectors (P6, P2, P3, P15,
P18, P4, P23, P5 and P1).
4.15-1
4
SERVICE AND REPAIR PROCEDURES
MOTOR/SOLENOID DRIVER CARD
4.
Close the lower chassis.
5.
Reconnect the ac power cable.
Figure 4.15-1 Motor/Solenoid Driver Card
J4
(To/from
DRA1, J1)
J18
(Resistor
bank)
J5
(To/from
DRA1, J2)
J2
(To/from
DRA2, J2)
J23
(Resistor
bank)
J15
(To +24 V
POWER ON)
J1
(To/from
DRA2, J1)
X1
(ON)
J6
(To/from
DRA1, J3)
J3
(To/from
DRA2, J3)
J11
(To/from Flex
Connect
card)
J8
(To
peristaltic
pump
motors)
J10
(To/from
Syringe
Assembly/
traverse
motor
J7
(To Solenoid
Interconnect card)
J12
(Not used)
MOTOR/SOLENOID DRIVER CARD
X2
(ON)
7242012A
Verification
4.15-2
1.
Turn ON the instrument’s power.
2.
Observe the operation of the aspirate and the diluent syringes while the system is going
through its power ON routine.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
MOTOR/SOLENOID DRIVER CARD
3.
When the system reaches the Main Menu, perform the Service Diagnostic (Heading 7.3)
and check for proper operation of all solenoids and the traverse motors.
4.
Verify the operation of the peristaltic pumps:
5.
PN 4237242C
a.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS.
b.
Select 4 RINSE to check PM3.
c.
Select 3 DRAIN to check PM4.
d.
Select 5 MIX to check PM1.
e.
Select 4 RINSE several times, until reservoir must refill, to check PM2.
Perform an SVP (Heading 5.1).
4.15-3
4
SERVICE AND REPAIR PROCEDURES
MOTOR/SOLENOID DRIVER CARD
4.15-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
SENSOR PREAMP ADAPTER (SPA) CARD
4.16
SENSOR PREAMP ADAPTER (SPA) CARD
Tools/Supplies Needed
B #1 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Open the lower chassis as directed under Heading 4.4 and locate the Sensor Preamp
Adapter card (Figure 4.4-1).
3.
Disconnect the electrical connectors located at the rear of the Sensor Preamp Adapter
card. There are five connectors (Figure 4.16-1) and three coaxial cables.
Figure 4.16-1 Sensor Preamp Adapter (SPA) Card and Surrounding Components
ELECTRICAL
CONNECTIONS
CABLE SHIELD
CARD SHIELD
GROUND
SCREW
SENSOR
PREAMP
ADAPTER
CARD
ELECTRODE
CABLES
7242040A
PN 4237242C
4.16-1
4
SERVICE AND REPAIR PROCEDURES
SENSOR PREAMP ADAPTER (SPA) CARD
4.
Remove the cable and card shield covers (Figure 4.16-1):
r
The cover for the cable shield is at the top of the “tunnel” between the Diluter Panel
and the Sensor Preamp Adapter card. It is held in place with two thumbscrews, one
on each side.
r
The cover for the card shield is held in place with two thumbscrews, one in each top
corner. The thumbscrews are accessed from the rear of the shield.
5.
Remove the ground screw for the card (Figure 4.16-1). This is a Phillips-head screw,
located in the left top corner of the card and removed from the rear.
6.
Remove the Sensor Preamp Adapter card by partially pulling up on the card until the
bath’s electrode cables (Figure 4.16-1) can be disconnected from the card. When the
cables are disconnected, the card is free from the instrument.
Installation
1.
r
X4 and X7 should be ON
r
X3 and X8 should be OFF.
2.
Install the replacement Sensor Preamp Adapter card into its shield. Connect the bath’s
electrode cables as the card is dropped into place, the WBC connector is below the RBC
connector (Figure 4.16-1).
3.
When the card is seated, fasten the card to the shield with the Phillips-head ground
screw (Figure 4.16-1). This screw is located just left of connector J7 and is threaded from
the rear.
4.
Install the cable and card shield covers (Figure 4.16-1):
5.
4.16-2
Before installing the Sensor Preamp Adapter card, check the jumpers (Figure 4.16-2):
r
The cover for the cable shield is at the top of the “tunnel” between the Diluter Panel
and the Sensor Preamp Adapter card. It is held in place with two thumbscrews, one
on each side.
r
The cover for the card shield is held in place with two thumbscrews, one in each top
corner. The thumbscrews are accessed from the rear of the shield.
Connect the five electrical connectors (Figure 4.16-1) and the three coaxial cables
located at the rear of the Sensor Preamp Adapter card.
r
The five connectors - J1 through J4 and J7. J5 and J6 are not used.
r
Three output coaxial cables - J9 is the lowest, J11 is the middle, J12 is the highest.
6.
Close the lower chassis.
7.
Turn ON the instrument’s power.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
SENSOR PREAMP ADAPTER (SPA) CARD
Figure 4.16-2 Sensor Preamp Adapter Card
J1
(From Linear
Power Supply
card)
J2
(From Linear
Power Supply
card)
J3
(Out to
URA)
J4
(Out to
SPAD)
J5
(Analog
voltage
spare)
J6
(Analog
voltage
spare)
J7
(Diagnostics)
X7
(ON)
X8
(OFF)
J11
(RBC signal
out to SPAD)
J12
(PLT
signal
out to
SPAD)
J10
(On back
of card)
J9
(WBC
signal
out to
SPAD)
R8
J8
(On back
of card)
R7
X4
(ON)
X3
(OFF)
7242013A
Verification
Perform an SVP (Heading 5.1) paying particular attention to backgrounds and control
recovery. If the instrument outputs histograms, verify that the control histograms appear as
expected.
PN 4237242C
4.16-3
4
SERVICE AND REPAIR PROCEDURES
SENSOR PREAMP ADAPTER (SPA) CARD
4.16-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
PROBE AND PROBE WIPE
4.17
PROBE AND PROBE WIPE
Tools/Supplies Needed
B #1 Phillips-head screwdriver
B Needle-nose pliers
B DOW CORNING 33® Lubricant, PN 1604007-0
Removal
1.
Using the keypad, proceed as if you were running a sample. When the probe moves to
the aspirate position, turn OFF the instrument’s power.
2.
Open the lower chassis, as directed under Heading 4.4, to completely expose the
Probe/Wipe Traverse Assembly.
3.
Remove the wire clip that retains the probe wipe housing in the traverse housing and
pull down the probe wipe housing (Figure 4.17-1).
Figure 4.17-1 Probe Assembly
PROBE/OPTO
SENSOR CARD
TRAVERSE RACK
PROBE
MOTOR
TRAVERSE
HOUSING
WIRE CLIP
WINDOW
SPRING
ROLLER
STABILIZING
SPRING
ASPIRATE
TUBING
O-RING
ASPIRATE
PROBE
7242022A
PN 4237242C
PROBE WIPE
HOUSING
4.17-1
4
SERVICE AND REPAIR PROCEDURES
PROBE AND PROBE WIPE
4.
If the probe wipe housing is the part to be replaced, disconnect the tubing and then
proceed to Installation, step 5.
5.
Remove the spring roller near the top of the traverse housing by removing the single
Phillips-head retaining screw (Figure 4.17-1).
6.
Remove the aspirate probe from the traverse rack (Figure 4.17-1):
a.
Move the aspirate probe until the top of the probe is visible through the window
opened by removing the spring roller in step 5 above.
b.
With needle-nose pliers or a flat-blade screwdriver, pry the aspirate probe down
from the traverse rack until it is clear of the rack. The aspirate probe is held in place
using a rubber O-ring to create a pressure fit.
7.
Pull the aspirate probe down until the aspirate tubing has emerged from the bottom of
the traverse housing (Figure 4.17-1).
8.
Remove the aspirate tubing from the aspirate probe, being careful not to misplace the
stabilizing spring (Figure 4.17-1).
Installation
1.
Ensure that the replacement aspirate probe has an O-ring. If it does not, use a new
O-ring; do not use the old one. It is a good idea to replace the O-ring any time the
aspirate probe has been removed. The O-ring deteriorates after several probe removals
and replacements, making it incapable of holding the probe in place.
2.
Install the aspirate tubing onto the probe (Figure 4.17-1):
a.
Remember to first put the stabilizing spring over the aspirate tubing.
b.
Make sure there is no grease or contamination on the aspirate probe fitting.
IMPORTANT Risk of damage to aspirate tubing. Pre-stretching the aspirate tubing could cause tears or an
improper fit that will compromise the instrument’s performance. The aspirate tubing fits without being
stretched. Do not pre-stretch the aspirate tubing.
c.
4.17-2
Push the aspirate tubing onto the aspirate probe.
3.
Lightly lubricate the O-ring using a silicon grease.
4.
Install the aspirate probe into the traverse rack (Figure 4.17-1):
a.
Move the traverse rack and aspirate probe until they are visible through the traverse
housing window.
b.
Hold the rack and using needle-nose pliers or a flat-blade screwdriver through the
window, force the aspirate probe up into the rack.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
PROBE AND PROBE WIPE
5.
Install the aspirate tubing onto the probe wipe housing (Figure 4.17-1) if it was
removed. The outside large bore tubing of the three-tube ribbon attaches to the top
fitting while the middle tubing attaches to the bottom. The top of the probe wipe
housing is the end with the groove for the wire retaining clip.
6.
Thread the probe wipe housing over the aspirate probe and into the traverse housing,
then install the wire retaining clip to hold it in place (Figure 4.17-1).
7.
Close the lower chassis.
8.
Turn ON the instrument’s power.
Verification
1.
2.
PN 4237242C
Run samples and ensure that:
r
The aspirate probe moves freely and remains in place.
r
There are no fluid leaks from the probe wipe and aspirate components.
Perform an SVP (Heading 5.1). Control recovery verifies that you have no leaks in the
aspirate tubing or its seal to the aspirate probe.
4.17-3
4
SERVICE AND REPAIR PROCEDURES
PROBE AND PROBE WIPE
4.17-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
PROBE MOTOR
4.18
PROBE MOTOR
Tools/Supplies Needed
B #1 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Open the lower chassis as directed in Heading 4.4, to expose the Probe/Wipe Traverse
Assembly.
3.
Disconnect the probe motor and the flex cable. The probe motor is connected to J2 on
the Probe/Opto Sensor card (Figure 4.17-1).
4.
Make sure the probe is in a vertical position. Move the traverse housing to the right end
of its travel. This aligns it with two probe motor access holes in the traverse mounting
bracket (Figure 4.19-1).
5.
Remove the probe motor. It is fastened to the traverse housing with two Phillips-head
screws.
Installation
1.
Install the replacement motor, making sure that the motor gear properly meshes with the
traverse rack.
2.
Fasten the motor using the two Phillips-head screws. There are screwdriver access holes
at the right end of the traverse mounting bracket (Figure 4.19-1).
3.
Connect the motor to J2 on the Probe/Opto Sensor card and reconnect the flex cable.
4.
Close the upper chassis and turn ON the instrument’s power.
Verification
Perform an SVP (Heading 5.1).
PN 4237242C
4.18-1
4
SERVICE AND REPAIR PROCEDURES
PROBE MOTOR
4.18-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
TRAVERSE DRIVE BELT
4.19
TRAVERSE DRIVE BELT
Tools/Supplies Needed
B #1 Phillips-head screwdriver
B Large flat-blade screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Open the lower chassis, as directed under Heading 4.4, to completely expose the
Probe/Wipe Traverse Assembly.
3.
Remove the belt from the idler pulley (Figure 4.19-1):
a.
Slide the traverse housing to the left to create more slack in the belt near the idler
pulley.
Note: There is no flange on the idler pulley. The belt slides off easily with slack.
b.
Slide the belt off the idler pulley.
Figure 4.19-1 Traverse Drive Belt
TRAVERSE
TENSIONER
BRACKET
TENSIONER
BRACKET/PULLEY
SCREW
TRAVERSE
MOUNTING
BRACKET
SPRING
TENSIONING
SCREW
PROBE MOTOR
ACCESS HOLES
IDLER
PULLEY
MOTOR
PULLEY
TENSIONER
PULLEY
SCREWDRIVER
ACCESS
CUTOUT
7242049A
PN 4237242C
CLAMP
4.19-1
4
SERVICE AND REPAIR PROCEDURES
TRAVERSE DRIVE BELT
4.
Remove the belt from the tensioner pulley (Figure 4.19-1):
a.
Slide the traverse housing to the right and loosen the screw that holds the tensioner
bracket and pulley in place (traverse belt tensioner bracket screw).
Note: You do not have to loosen the traverse belt spring tensioning screw. The
tension is set at the factory and should not need to be altered.
b.
Remove the belt from the tensioner pulley by forcing the tensioner bracket right,
against the tension spring, until there is enough slack in the belt to slide it over the
pulley's flange.
Note: A large flat-blade screwdriver can be used to pry the tensioner against the
spring pressure.
5.
Remove the belt from the unit (Figure 4.19-1):
a.
Locate the screwdriver access cutout in the lower left portion of the traverse
mounting bracket.
b.
Loosen the #1 Phillips-head screw that clamps the belt to the traverse housing just
enough to allow the belt to be removed from the clamp.
c.
Remove the belt. It is now completely free of the instrument.
Installation
4.19-2
1.
Clamp the new belt to the traverse housing (Figure 4.19-1).
2.
Install the belt onto its pulleys (Figure 4.19-1):
r
Loop the right end of the belt over the motor pulley.
r
Loop the left end of the belt over the tensioner pulley.
r
Slide the belt over the idler pulley.
3.
Seat the traverse tensioner bracket and move the traverse housing back and forth to fully
seat the belt (Figure 4.19-1). Once fully seated, tighten the tensioner bracket/pulley
screw. The spring will have set the proper belt tension.
4.
If the spring tensioning screw was moved, establish proper tension by turning the screw
until the screw is just coming out of the threaded bracket. The acceptable tolerance is
one to two threads exposed.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
TRAVERSE DRIVE BELT
CAUTION Risk of component damage. Clamping the belt clamp too far forward or backward produces a
bend during normal operation that prematurely wears the belt, traverse housing and traverse guide rod.
Tighten the belt clamp on the same plane as the motor pulley to avoid premature wear on the belt, traverse
housing and traverse guide rod.
5.
Make sure the belt is clamped on the same plane as the motor pulley (Figure 4.19-1). If it
is not, loosen the belt and reposition it.
6.
Close the lower chassis and turn ON the instrument’s power.
Verification
Perform an SVP (Heading 5.1). While the system is cycling, view the belt from the right-hand
side to ensure there are no bends. Adjust if necessary by loosening the clamp and moving
the belt.
PN 4237242C
4.19-3
4
SERVICE AND REPAIR PROCEDURES
TRAVERSE DRIVE BELT
4.19-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
TRAVERSE MOTOR
4.20
TRAVERSE MOTOR
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B 1/4 in. nut driver
B DOW CORNING 33 Lubricant, PN 1604007-D
Removal
1.
Turn OFF the instrument’s power.
2.
Open the lower chassis, as directed under Heading 4.4, to completely expose the
Probe/Wipe Traverse Assembly.
3.
Remove the access cover at the top of the Syringe Assembly (Figure 4.20-1).
Figure 4.20-1 Traverse Motor
REAR OF TRAVERSE ASSEMBLY
ACCESS
COVER
TRAVERSE
MOTOR
VACUUM
REGULATOR
RIBBON
TUBING
TRAVERSE
MOUNTING
BRACKET
PHILLIPS-HEAD
SCREWS
MOUNTING
POSTS
FLATHEAD
SCREW
PN 4237242C
7242041A
4.20-1
4
SERVICE AND REPAIR PROCEDURES
TRAVERSE MOTOR
4.
Disconnect the traverse motor. The traverse motor connector is to the right
(Figure 4.20-1) and is labeled as “J5" and “TRAV MOTOR.”
5.
Disconnect the following cables, making sure that these cables are free and can be
removed from the instrument:
6.
r
J15, J16 and J17 (sensor cables from the Solenoid Interconnect card)
r
J1 flex cable (from the Flex Connect card).
Disconnect the ribbon tubing that attaches the Traverse Assembly to the fluidics system
(Figure 4.20-1):
r
The small aspirate tubing connects to the aspirate syringe manifold.
r
The middle tubing connects to port #2 of LV8.
r
The large bore, outside tubing connects to the normally open port of LV16.
7.
Remove the five fasteners attaching the Traverse Assembly to the lower chassis. There are
three Phillips-head screws, two on the extreme right edge of the traverse mounting
bracket and one Phillips-flathead screw just above the right corner of the tensioner
bracket. The last two fasteners are 1/4 in. nuts located about 4 in. from each end of the
traverse mounting bracket.
8.
With the Traverse Assembly on the bench, remove the traverse motor from the traverse
mounting bracket by removing the four retaining screws (Figure 4.20-1).
Installation
Before installing the replacement motor, make sure that it has a wear plate and a rubber seal.
The wear plate is attached to the motor with black RTV compound and the rubber seal is
installed concave end toward the wear plate and lubricated with DOW CORNING 33 Lubricant.
4.20-2
1.
Attach the traverse motor to the traverse mounting bracket with the four retaining
screws (Figure 4.20-1).
2.
3.
Hang the Traverse Assembly onto the two mounting posts, threading the connector
cables through the lower chassis at the same time.
Attach and tighten the three Phillips-head screws (Figure 4.20-1).
4.
Attach and tighten the two 1/4-in. nuts onto the mounting posts (Figure 4.20-1).
5.
Connect the ribbon tubing to the fluidics system (Figure 4.20-1):
r
The small aspirate tubing attaches to the long special fitting at the front of the
aspirate syringe manifold.
r
The middle tubing attaches to the #2 port of LV8.
r
The larger bore outside tubing attaches to the normally open port of LV16.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
TRAVERSE MOTOR
6.
7.
Connect the electrical connectors:
r
Flex cable (from Probe/Opto Sensor card) to J1 (on Flex Connect card).
r
Traverse motor to J5 (behind the Syringe Assembly).
r
Aspirate position sensor to J17 (on Solenoid Interconnect card).
r
WBC position sensor to J15 (on Solenoid Interconnect card).
r
RBC position sensor to J16 (on Solenoid Interconnect card).
Replace the access cover, lower the upper chassis and turn ON the instrument’s power.
Verification
Perform an SVP (Heading 5.1).
PN 4237242C
4.20-3
4
SERVICE AND REPAIR PROCEDURES
TRAVERSE MOTOR
4.20-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
SYRINGE ASSEMBLY
4.21
SYRINGE ASSEMBLY
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B 1/4 in. HEX-nut driver
B 1/16 in. HEX-key wrench
B Large flat-blade screwdriver
B LOCTITE® 242 THREADLOCKE2 adhesive, PN 1601018-9
Removal
1.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 2 REPLACE SYRINGE.
2.
Turn OFF the instrument’s power.
3.
Open the lower chassis, as directed under Heading 4.4, to completely expose the
Probe/Wipe Traverse Assembly.
4.
Remove the Phillips-head screw (in the center) that secures the metal shield. Remove the
metal shield that covers the lower portion of the syringes.
5.
Remove the 1/4-in. HEX-head screws at the bottom of each plunger (Figure 4.21-1).
6.
Unscrew the syringe bodies from the Luer fittings (Figure 4.21-1).
7.
Remove the door from the Syringe Assembly by removing the hinge bracket
(Figure 4.21-1) from the Syringe Assembly. It is fastened to the instrument with three
Phillips-head screws and one nut.
8.
Remove the Syringe Assembly from the unit. Four Phillips-head screws remain that
fasten the Syringe Assembly to the lower chassis (Figure 4.21-1).
9.
r
Two vertically-oriented screws are threaded to the floor of the lower chassis.
r
Two screws remain along the top of the assembly, one left and one center.
Attach a hemostat to the diluent tube.
10. Slide the assembly out of the lower chassis:
a.
When it is far enough out, remove the three tubes connected to the syringe
manifolds.
b.
Slide the assembly out further until the cables can be disconnected. There are four
cables and they connect to the rear wall of the syringe cavity.
11. Loosen the screws fastening the motor associated with the syringe components that you
are replacing. If the motor is to be replaced, remove the screws, being careful not to lose
standoffs and nuts.
PN 4237242C
4.21-1
4
SERVICE AND REPAIR PROCEDURES
SYRINGE ASSEMBLY
Figure 4.21-1 Syringe Assembly
SYRINGE
MANIFOLDS
LUER
FITTING
(2)
HINGE
BRACKET
HEX-HEAD
SCREW
(2)
SYRINGE
GUIDE ROD
SCREWS
UPPER
BEARING
UPPER
MOUNTING
PLATE
SPACERS
SPRING
LEAD
SCREW
LEAD
SCREW
ASSEMBLY
SYRINGE
PLUNGER
MOUNTING
BRACKET
GUIDE ROD
GEAR
MOTOR
SPACER
LOWER
BEARING
SCREW
STANDOFF
NUT
(4)
LOOSEN
BELT
4.21-2
7242051A
PN 4237242C
SERVICE AND REPAIR PROCEDURES
SYRINGE ASSEMBLY
12. Free the syringe lead screw assemblies (Figure 4.21-1):
a.
Remove the two screws fastening the upper mounting plate to the top of the front
plate.
b.
Remove the two screws fastening the syringe guide rods to the upper mounting
plate.
c.
Remove the upper mounting plate with manifolds and sensors still attached.
ATTENTION: There are two spacers and one spring for each syringe. Do not lose them, they are
critical to syringe operation.
13. Remove the hardware at the top of each syringe and set aside.
14. Remove the belt from the motor gear first, then the lead screw gear. There will be enough
play in the motor assembly and lead screw assembly to work it off.
15. If the lead screw assembly is to be replaced, loosen the two screws that fasten the front
plate to the lower mounting plate (Figure 4.21-1). This will allow the lead screw
assembly to be pulled up and away from the Syringe Assembly.
16. Remove the lower bearing, spacer and gear from the bottom of the lead screw
(Figure 4.21-1).
17. Remove the syringe plunger mounting bracket from the lead screw assembly.
Installation
1.
Install the syringe plunger mounting bracket, lead screw gear, spacer and lower bearing
onto the replacement lead screw assembly (Figure 4.21-1):
ATTENTION: The thickness of the spacer is very important. If it is lost, replace it with the
proper part.
PN 4237242C
r
Push the gear onto the lead screw as far as it will go.
r
Before tightening the setscrews, tighten one against the flat part of the lead screw
shaft.
r
Before tightening the screws, use LOCTITE 242 THREADLOCKE2 on the
Phillips-head screw that fastens the bearing to the lead screw.
2.
If the motor had been removed, install the motor at this time. Do not tighten the motor
in place yet, it is used to adjust belt tension.
3.
Install the replacement lead screw assembly into the Syringe Assembly. Do not fasten yet.
4.
Install the drive belt by putting the belt around the lead screw gear first, then around the
motor gear.
4.21-3
4
SERVICE AND REPAIR PROCEDURES
SYRINGE ASSEMBLY
5.
Install the upper mounting plate (Figure 4.21-1):
a.
Replace the upper lead screw hardware, with a spacer going on first, then the spring,
then another spacer.
b.
Place the upper mounting plate onto the two syringe lead screws and fasten to the
two guide rods. Tighten each screw a bit at a time until both are tight.
c.
Check that the drive belt is still around both gear assemblies. If it is, fasten the
upper mounting plate to the face plate.
d.
Tighten all six face plate screws at this time.
6.
Tighten the motor assembly to the proper belt tension. Belt tension is set to 4 lb at the
factory. An acceptable alternative is to tighten the belt such that easy finger pressure to
the belt will push it half way, or about 1/4 in. toward the belt on the other side of the
loop.
7.
Verify that the belt gears are at the same level so that the belt is not at an angle. If an
adjustment is required, change the position of the motor gear.
8.
Install the Syringe Assembly into the instrument:
9.
a.
Connect the four electrical connectors, P1 through P4.
b.
Connect the three fluid lines.
c.
Fasten the assembly to the instrument. Four Phillips-head screws fasten the Syringe
Assembly to the lower chassis (Figure 4.21-1).
r
Two vertically-oriented screws are threaded to the floor of the lower chassis.
r
Two screws remain along the top of the assembly, one left and one center.
Fasten the hinge bracket (Figure 4.21-1) and the door to the Syringe Assembly using
three Phillips-head screws and one nut.
Verification
4.21-4
1.
Turn ON the instrument’s power.
2.
Observe the Syringe Assembly during power up.
3.
If no problems are observed, run a prime cycle to prime the system with diluent. Observe
the syringe area for smooth movement of the syringes and for fluid leaks.
4.
If no problems are observed, replace the metal shield that covers the lower syringe area.
5.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
PERISTALTIC PUMP MOTOR AND SPOOL
4.22
PERISTALTIC PUMP MOTOR AND SPOOL
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B 1/16 in. HEX-key wrench
B DOW CORNING 33 Lubricant, PN 1604007-0
Removal
1.
Turn OFF the instrument’s power.
2.
Access the Peristaltic Pump Assembly:
a.
Open the small door on the left side of the unit that holds the diluent reservoir
(Figure 4.22-1).
Figure 4.22-1 Peristaltic Pump Motors
PERISTALTIC
PUMP MOTOR
(4)
TUBING
SPOOL
PERISTALTIC
TUBING
7242023A
PN 4237242C
b.
Disconnect the diluent sensor connector and lift the door off its hinges.
c.
Prop the door out of the way toward the rear of the instrument, keeping in mind
that the diluent tank will spill any fluid it contains, if laid on its side.
4.22-1
4
SERVICE AND REPAIR PROCEDURES
PERISTALTIC PUMP MOTOR AND SPOOL
3.
Remove the Peristaltic Pump Assembly from the lower chassis. It is held in place with
six Phillips-head screws (Figure 4.22-1). There should be enough play in the tubing and
wiring to allow work on the assembly without disconnecting it.
4.
Remove the peristaltic tubing from the pump (Figure 4.22-1).
5.
Remove the tubing spool from the motor shaft (Figure 4.22-1). This spool is fastened
with two HEX-head setscrews that tighten against the shaft. If you are replacing the
motor, this step could be performed after you remove the motor, since the opening in the
mounting plate will allow the spool through. If you are replacing the spool, go to
Installation, step 3, below.
6.
Disconnect the electrical connector at the back of the motor you wish to replace.
7.
Disconnect the four Phillips-head screws with nuts (Figure 4.22-1) and remove the
motor.
Installation
1.
Make sure the replacement motor has a wear plate and a rubber seal. The wear plate is
attached to the motor with black RTV compound and the rubber seal is installed concave
end toward the wear plate and lubricated with DOW CORNING 33 Lubricant.
2.
Attach the motor using the four Phillips-head screws and nuts (Figure 4.22-1).
3.
Attach the tubing spool if it has not already been attached (Figure 4.22-1). The tubing
spool is fastened to the motor shaft using two HEX-head setscrews that must line up
with the flattened portion of the motor shaft. The spool is pushed onto the shaft as far as
it will go.
4.
Attach the Peristaltic Pump Assembly to the lower chassis using six Phillips-head screws
(Figure 4.22-1).
5.
Reconnect any motors that were disconnected.
6.
Install the diluent reservoir door onto the lower chassis (Figure 4.22-1).
7.
Connect the diluent level sensor and wind the peristaltic tubing onto the pump spool.
8.
Turn ON the instrument’s power.
Verification
1.
2.
4.22-2
Verify the operation of the peristaltic pumps:
a.
From the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS.
b.
Select 4 RINSE to check PM3.
c.
Select 3 DRAIN to check PM4.
d.
Select 5 MIX to check PM1.
e.
Select 4 RINSE several times, until reservoir must refill, to check PM2.
Perform an SVP (Heading 5.1).
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
VACUUM PUMP
4.23
VACUUM PUMP
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Disconnect the ac power cable.
3.
Open the lower chassis, as directed under Heading 4.4, and locate the vacuum pump
(Figure 4.4-1).
4.
Disconnect the electrical connector for the vacuum pump.
5.
Disconnect the single tubing connected to the left end of the pump.
6.
Remove the vacuum pump from the instrument. It is attached to the lower chassis floor
with four Phillips-head screws.
Installation
1.
Check that there is a right-angle fitting on the vent port. This port is located on top of
the pump.
2.
Connect the diluter tubing to the end port of the vacuum pump, and the electrical input
cable to the power supply cable that comes from J7 on the AC Power/Vacuum Relay card.
3.
Fasten the pump to the floor of the lower chassis using four Phillips-head screws. Make
sure the output port of the pump faces left.
4.
Close the lower chassis, reconnect the ac power cable and turn ON the instrument’s
power.
Verification
PN 4237242C
1.
Perform a Vacuum Adjustment (Heading 4.30).
2.
Run several sample cycles without aspirating anything.
3.
Check for any fluid leaks from the probe wipe. Raw pump vacuum is used to evacuate
fluids from the probe wipe housing and you must verify that there is sufficient vacuum to
accomplish this.
4.
Perform an SVP (Heading 5.1).
4.23-1
4
SERVICE AND REPAIR PROCEDURES
VACUUM PUMP
4.23-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
APERTURE, BATH AND APERTURE ELECTRODE MODULE
4.24
APERTURE, BATH AND APERTURE ELECTRODE MODULE
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Drain the baths.
2.
Turn OFF the instrument’s power.
3.
Open the lower chassis as directed in Heading 4.4.
4.
Gain access to the aperture area by removing the Diluter Panel shield with door. The
shield is held in place with three captive thumbscrews.
5.
Remove the three tubes connected to the bottom of each bath.
6.
Disconnect the external electrode connector (Figure 4.24-1). This is a single pin
MATE-N-LOK® connector coming from the top of each bath and going to the shield wire
of the Aperture Electrode module coaxial cable
Figure 4.24-1 Aperture Bath Assembly
APERTURE
BLOCK
APERTURE
ELECTRODE
MODULE
EXTERNAL
ELECTRODE
CONNECTOR
SENSOR
PREAMP
CABLES
APERTURE
BATH
(WBC)
O-RING
METAL
INSERT
THUMBSCREW
APERTURE
BATH
(RBC)
7242024A
PN 4237242C
4.24-1
4
SERVICE AND REPAIR PROCEDURES
APERTURE, BATH AND APERTURE ELECTRODE MODULE
7.
Remove the thumbscrew at the front of the aperture bath assembly (Figure 4.24-1). The
bath, aperture, and rear module are pressure-fit and this thumbscrew provides the pressure.
8.
Remove the aperture bath. To get enough room at the front of the bath to clear the
aperture block, you may have to remove the metal insert (Figure 4.24-1) that the
thumbscrew presses against.
9.
If you are replacing the bath only, go to Installation, step 2.
10. If you are replacing the RBC aperture block, remove the sweep-flow tubing. Loosen the
fitting that pushes up against the aperture from underneath. This allows the aperture
block to be easily pulled out of the Aperture Electrode module.
11. Remove the aperture blocks from the Aperture Electrode module (Figure 4.24-1). The
Aperture Electrode module is not attached and can be moved to a convenient working position.
12. If you are not going to replace the Aperture Electrode module, go to Installation, step 3
of this section.
13. Open up the lower chassis and raise the upper chassis.
14. Remove the two covers that shield the Sensor Preamp Adapter card and the electrode
cables. See the replacement procedures (Heading 4.16) for details on removing these
covers.
15. Slide the Sensor Preamp Adapter card up until you can remove the electrode cable for the
Aperture Electrode module you are replacing.
16. Remove the Aperture Electrode module by pulling the cable through the Diluter Panel
(Figure 4.24-1).
Installation
1.
4.24-2
Install the new Aperture Electrode module into the unit:
a.
Feed the coaxial cable through the hole in the Diluter Panel (Figure 4.24-1).
b.
Slide the Sensor Preamp Adapter card up until you can connect the coaxial cable to
its proper connector. The lower connector is for the WBC module and the upper
connector is for the RBC module.
c.
Replace the shield covers for the Sensor Preamp Adapter card and the electrode cables.
d.
Close the upper chassis.
PN 4237242C
SERVICE AND REPAIR PROCEDURES
APERTURE, BATH AND APERTURE ELECTRODE MODULE
2.
3.
4.
Install the aperture block (Figure 4.24-1):
a.
Verify that there are small O-rings at the top and bottom of the block.
b.
Verify that the O-rings are in good condition. If they are too flat or beginning to
deteriorate, replace them.
c.
Verify that the area around the aperture bath is dry.
d.
Tighten the fitting below the aperture block. It only needs to be snug, or tight
enough to ensure that the O-ring seals are compressed.
Install the aperture bath (Figure 4.24-1):
a.
Verify that the large aperture O-ring seal is seated in its groove in the bath. This seal
will be pressure-fit against the front of the aperture block.
b.
Position the Aperture Electrode module against the rear pressure plate.
c.
Slide the bath in place until it can be fit onto the aperture block.
d.
Set the metal insert (front pressure plate) in place and tighten the thumbscrew
against it. This should be a snug fit, only finger-tight.
Connect the bath electrode (external electrode connector) to the connector on the
Aperture Electrode module (Figure 4.24-1).
Verification
PN 4237242C
1.
Turn ON the instrument’s power.
2.
Perform a startup. Observe the bath and aperture area closely for any fluid leaks or air
leaks in the sweep flow or internal electrode path.
3.
Replace the inner shield door and close the lower chassis.
4.
If there are no visible problems, perform an SVP (Heading 5.1).
4.24-3
4
SERVICE AND REPAIR PROCEDURES
APERTURE, BATH AND APERTURE ELECTRODE MODULE
4.24-4
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
DILUTER PANEL SOLENOIDS
4.25
DILUTER PANEL SOLENOIDS
Tools/Supplies Needed
B #2 Phillips-head screwdriver
Removal
1.
Turn OFF the instrument’s power.
2.
Open the lower chassis as directed in Heading 4.4.
3.
Locate the Solenoid Interconnect card (Figure 4.4-1).
4.
Disconnect the connector for the solenoid you are replacing and free the cable of any
wire ties or obstructions so that the connector can easily be pulled through the Diluter
Panel from the front.
5.
If there is an extension cable on the solenoid (LV5, LV11), remove it and install it on the
replacement solenoid.
6.
Remove the Diluter Panel shield with door. The shield is held in place with three captive
thumbscrews.
7.
Remove the two screws that hold the solenoid onto the Diluter Panel. The screws are
visible from the front and there is no loose hardware to contain when removing the
screws.
8.
Pull the solenoid out of the Diluter Panel being careful not to snag the connector.
Installation
1.
Push the wiring and connector for the new solenoid through the Diluter Panel
mounting, hole and with two screws fasten the solenoid to the Diluter Panel.
2.
Locate the connector (using a hemostat if necessary) and connect the cable to the
Solenoid Interconnect card.
3.
Replace the Diluter Panel shield with door, with three captive thumbscrews.
4.
Turn On the instrument’s power.
Verification
PN 4237242C
1.
Run the Service Diagnostic in Table 7.3-1, to verify solenoid operation. Putting a finger
on the small plunger at the front of the solenoid helps verify mechanical activation.
2.
Perform an SVP (Heading 5.1).
4.25-1
4
SERVICE AND REPAIR PROCEDURES
DILUTER PANEL SOLENOIDS
4.25-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
SWEEP-FLOW TUBING
4.26
SWEEP-FLOW TUBING
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B Needle-nose pliers
B Small-side cutter
Removal
1.
Open the lower chassis as directed in Heading 4.4.
2.
Gain access to the aperture area by removing the Diluter Panel shield with door. The
shield is held in place with three captive thumbscrews.
3.
Disconnect the sweep-flow tubing. One end is attached to port 1 of LV4 and the other is
attached to the RBC aperture sweep-flow fitting on the Diluter Panel (Figure 4.26-1).
ATTENTION: Do not remove the upper left and lower right screws. This will release the box
shield attached to the back of the Diluter Panel.
4.
Remove the face plate covering the sweep-flow tubing spool that is held in place by two
Phillips-head screws, one lower left and one upper right (Figure 4.26-1).
5.
Remove the sweep-flow spool with tubing (Figure 4.26-1). It is not attached.
Figure 4.26-1 Sweep-Flow Tubing
DILUTER PANEL
SWEEP-FLOW
TUBING
SPOOL
FACE
PLATE
LV4
7242025A
PN 4237242C
4.26-1
4
SERVICE AND REPAIR PROCEDURES
SWEEP-FLOW TUBING
Installation
1.
Wind 13 ft (evenly back and forth) of PN 3202220-1 tubing onto the spool, being careful
to leave 4 in. at one end and 5 in. at the other end. The tubing must all fit in the spool
when wound.
2.
Thread the exposed ends of the tubing through the face plate and place the spool in its
cavity (Figure 4.26-1).
3.
Fasten the face plate to the Diluter Panel (Figure 4.26-1).
4.
Connect the 4 in. tubing lead to the RBC aperture sweep-flow fitting and the 5 in. lead to
port 1 of LV4 (Figure 4.26-1).
5.
Close the lower chassis.
Verification
1.
Prime the sweep flow.
Note: Instrument cycles may not be sufficient in some cases. When that happens, use the
syringe to prime the sweep flow.
2.
4.26-2
Perform an SVP (Heading 5.1), paying close attention to Plt results and histograms. If
there is a sweep-flow problem, you will see a large spike at the beginning of the
histogram.
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
LATEX GAIN ADJUSTMENT
4.27
LATEX GAIN ADJUSTMENT
Tools/Supplies Needed
B Latex aperture gain particles, PN 6857371-8
Procedure
1.
Ensure that the instrument is primed and ready to run a sample.
2.
At the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 1 LATEX GAIN.
3.
Enter the latex particle size from the assay value stated on the container.
4.
Make sure the latex aperture gain particles are well mixed, then aspirate them as directed
on the display.
5.
Compare the WBC and RBC results to the latex assay value. A display of Counts, CVs
and Sizes is given for WBC and RBC.
6.
7.
PN 4237242C
r
Verify that the RBC CV is ≤8 and the WBC CV is ≤16. Results not meeting this
criteria should not be used for adjustments.
r
Adjustment is necessary if either parameter is not within 1.5 fL of the latex assay
value. Press Enter to advance to the Adjustment menu.
The Adjustment menu displays the new aperture current setting.
r
Do not adjust the WBC if is it is outside the range of 99.6 to 140.4 V. Do not adjust
the RBC if is it is outside the range of 124.5 to 175.5 V.
r
If you cannot adjust within range, there is a problem that requires correction.
r
To leave the Adjustment menu without making an adjustment, press Escape.
Run controls to verify calibration. Changes in aperture gain affect MCV and MPV the
most. Histogram shifts also occur.
4.27-1
4
SERVICE AND REPAIR PROCEDURES
LATEX GAIN ADJUSTMENT
4.27-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
AIM ADJUSTMENT
4.28
AIM ADJUSTMENT
Tools/Supplies Needed
B Five different whole-blood specimens with normal parameters (especially for WBC)
Note: Since nomal parameters do not guarantee a normal AIM result, it is preferable to
use different blood.
Procedure
1.
Bleach the apertures. Refer to the How to Clean the Baths procedure in the Operator’s
Guide.
2.
Run each of the five specimens once. After each sample, access the Service Report (see
Heading 7.2, GENERATING A SERVICE REPORT), and print or record the measured
26th-percentile AIM reading for RBC and WBC.
3.
Average the five, measured, 26th-percentile readings for RBC and WBC. These results are
your new “target values.”
4.
Verify that the RBC Target (T) value falls within the 2150 to 2550 range and that the
WBC Target (T) value falls withing the 2100 to 2500 range. If either are outside of these
ranges, there is an instrument problem that must be resolved before continuing.
5.
If the new target values are within ±25 of the current (old) target values, do not adjust.
If adjustment is necessary, access the Service Report screen (see Heading 7.2,
GENERATING A SERVICE REPORT), and enter the new target values.
Optional Procedures
Option 1
Controls and calibrators have different physical properties from fresh whole blood. The 26th
percentile readings that they produce can also differ, and generate AIM alerts if the differences
are great enough. If the WBC 26th percentile is too low, as is the case with COULTER 4C®
PLUS Abnormal Low cell control, target adjustments up to 2% are allowed. There is no
allowance for the WBC 96th percentile reading being too high, or for the RBC 26th percentile
reading being too high or too low.
PN 4237242C
1.
Obtain a WBC 26th percentile average for fresh whole blood, following the procedure
above.
2.
Run the control (Abnormal Low if 4C cell control is being used) five times and average
the WBC 26th percentile readings.
3.
Average the fresh blood average and the control average. This is the same as averaging all
10 runs. Compare this result to the fresh blood average less 2% (fresh blood average x
0.980). Adjust to the higher value.
4.
If 4C Abnormal Low cell control was used, it is acceptable to adjust to 1.02 x the 4C
Abnormal Low cell control average.
4.28-1
4
SERVICE AND REPAIR PROCEDURES
AIM ADJUSTMENT
Option 2
Many customers in the MD II market do not have samples of blood at installation, or have
only fingerpick samples, which are not ideal for AIM adjustment. Though not preferred, 4C
PLUS cell control can be used to adjust the AIM values.
This method produces an RBC 26th percentile target similar to the preferred fresh
whole-blood results. For WBC, the value is about 1.5% lower than the value produced using
fresh whole blood. Since the low control is generally about 3.0% below the AIM target for
fresh blood, using this lower 26th percentile value is an acceptable compromise. If a value
matching fresh whole blood is desired, multiply the average WBC obtained value by 1.015.
4.28-2
1.
Run the high control five times. Print or record the WBC and RBC measured 26
percentile value after each run.
2.
Average the five WBC and the five RBC 26th percentile readings.
3.
If the averages are within ±25 of the instrument target values, do not adjust. If the
averages are outside this range, adjustment is required. Adjust to the average values.
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
HGB PREAMP ADJUSTMENT
4.29
HGB PREAMP ADJUSTMENT
Tools/Supplies Needed
B #2 Phillips-head screwdriver
B DVM with a 3 1/2 digit display
B Pot adjustment tool or small-blade screwdriver
Procedure
1.
Ensure the system is primed and that there is clear diluent in the WBC bath.
2.
Open the lower chassis, as directed under Heading 4.4, and locate the Hgb Preamp card
(Figure 4.4-1).
3.
Adjust the Hgb-blank reading. Using TP2 or the chassis ground for the negative lead and
TP1 for the positive lead, adjust R7 for a DVM reading of 4.5 V.
ATTENTION: When covering the photodetector, make sure no light can get to the detector.
Remove it from the WBC bath if necessary.
4.
Adjust the Hgb Zero Offset. Cover the photodetector so that it receives no light and
adjust R8 until a reading of 0.0 ±1 mV is attained.
5.
Restore the Hgb system and check the original Hgb-blank reading. If it has changed by
more than 0.05 V, repeat the blank and zero adjustments.
6.
Restore the instrument to operating status and perform an SVP (Heading 5.1).
Note: Though a DVM is preferable, this adjustment can be performed using the service
voltage display.
PN 4237242C
4.29-1
4
SERVICE AND REPAIR PROCEDURES
HGB PREAMP ADJUSTMENT
4.29-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
VACUUM ADJUSTMENT
4.30
VACUUM ADJUSTMENT
Procedure
PN 4237242C
1.
At the Main Menu, select 5 SPECIAL FUNCTIONS tt 5 SERVICE tt 5 VACUUM ADJUST.
2.
Using the vacuum regulator (Figure 4.20-1) in the upper-right corner of the fluidics
panel, adjust as close to 6.00 in. Hg as possible. The system tolerance limit for vacuum is
5.83 to 6.17 in. Hg.
4.30-1
4
SERVICE AND REPAIR PROCEDURES
VACUUM ADJUSTMENT
4.30-2
PN 4237242C
C
SERVICE AND REPAIR PROCEDURES
LYSE VOLUME ADJUSTMENT/VERIFICATION
4.31
LYSE VOLUME ADJUSTMENT/VERIFICATION
Note: This procedure need not be performed frequently. The solenoid pump is very accurate
and stable. Once adjusted correctly, it should not require readjustment. Any drift or variability
in the volume is most likely caused by a problem elsewhere in the lyse system.
Tools/Supplies Needed
B Lyse volume-adjustment measuring vial, PN 5415483-6, or a 5-mL graduated measuring
device
B 9/16 in. or adjustable wrench
B Large (1/4 in.) flat-blade screwdriver
Procedure
1.
At the Main Menu, select 5 SPECIAL FUNCTIONS tt 3 DILUTER FUNCTIONS tt 3 DRAIN.
2.
Disconnect the lyse pump output tubing from the Y-fitting below the WBC bath.
3.
Insert the open end of the output tubing into a waste container.
4.
At the Diluter Functions menu, select 2 DISPENSE LYSE. Dispense lyse once if the system
is primed, or as many times as necessary to prime the system if it is unprimed.
5.
When there are no bubbles in the lyse system, insert the output tubing into an empty
measuring vial and dispense lyse 10 times.
6.
Check the meniscus of the lyse:
7.
r
If the meniscus does not fall within the two indicating lines on the vial (the lines
represent 4100 and 4200 µL), adjust the lyse pump. Go to step 7.
r
If the meniscus is within the two indicating lines on the vial, go to step 11.
To adjust the pump:
a.
Loosen the adjustment screw locknut at the bottom of the lyse pump.
b.
Turn the adjustment screw clockwise to decrease volume or counterclockwise to
increase volume.
Note: The adjustment is very coarse, approximately 80 µL for each turn of the screw.
8.
When satisfied with the adjustment, tighten the locknut, trying not to turn the
adjustment screw.
9.
Rinse and dry the vial.
10. Dispense lyse once into the waste container, then dispense 10 times into the empty
measuring vial to verify correct volume. Repeat the adjustment if the volume is incorrect.
11. Reconnect the lyse tubing, dispense lyse twice, then select 4 RINSE.
12. Perform an SVP (Heading 5.1).
PN 4237242C
4.31-1
4
SERVICE AND REPAIR PROCEDURES
LYSE VOLUME ADJUSTMENT/VERIFICATION
4.31-2
PN 4237242C
C
CONTENTS
5
MAINTENANCE PROCEDURES, 5.1-1
5.1
5.2
PN 4237242C
SYSTEM VERIFICATION PROCEDURE (SVP), 5.1-1
PMI RECOMMENDATIONS, 5.2-1
Components, 5.2-1
Filters, 5.2-1
Peristaltic Pump Tubing, 5.2-1
Polyurethane Tubing, 5.2-1
Syringes, 5.2-1
Cleaning, 5.2-1
Adjustment Procedures, 5.2-2
Verification Procedures, 5.2-2
5-i
5
CONTENTS
5-ii
PN 4237242C
C
5MAINTENANCE PROCEDURES 5
5.1
SYSTEM VERIFICATION PROCEDURE (SVP)
PN 4237242C
1.
Inspect the blue and green filters and change if required. Peristaltic pump tubing wear
requires that the blue filters connected to the diluent and rinse pumps should be
changed every 9,000 cycles.
2.
Inspect the peristaltic pump tubing. If it shows excessive wear, or it has been in service
for 18,000 cycles, the tubing should be replaced. If the diluent or rinse pump tubing is
changed, the blue filter associated with that pump should also be changed.
3.
Check the aspirate and diluent syringes. If there is evidence of excessive leaking or if
they have been in service for 35,000 cycles, they should be replaced.
4.
Check the Hgb-blank voltage and adjust if necessary.
5.
Ensure that the Verification section has been completed for any replacement procedures
that have been performed.
6.
Perform the REPRODUCIBILITY AND CARRYOVER function on the CALIBRATION MENU
(Operator’s Guide, under the Calibration heading in the Replace/Adjust section). Both
these tests must PASS.
7.
Run all levels of control and verify that they are within expected limits.
5.1-1
MAINTENANCE PROCEDURES
SYSTEM VERIFICATION PROCEDURE (SVP)
5.1-2
PN 4237242C
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MAINTENANCE PROCEDURES
PMI RECOMMENDATIONS
5.2
PMI RECOMMENDATIONS
The MD II is a low maintenance instrument that does not require a PMI. Those service
organizations that will perform PMIs on this instrument can consider the following
components and verifications for their procedure.
Components
Filters
The green fluid barriers and blue particle filters should be replaced on every PMI. The
effectiveness of these filters is determined by environmental and instrument conditions more
than by instrument cycle count.
Aged peristaltic pump tubing or contaminated diluent determine replacement of the blue
particle filter. Diluter problems, especially those associated with the VIC, determine
replacement of the green fluid barriers.
Peristaltic Pump Tubing
The peristaltic pump tubing should be changed every 18,000 instrument cycles. Find out the
customer's usage rate to decide whether this tubing should be changed every PMI or not.
Polyurethane Tubing
Polyurethane tubing should be changed when it loses its elasticity. When the tubing becomes
stiff and discolored it has lost its elasticity.
Syringes
The diluent and aspirate syringes should be changed every 35,000 instrument cycles or when
by visual inspection you can see that it needs changing. Even though discoloration caused by
rusting of the shaft may occur before 35,000 instrument cycles, performance should not be
affected.
Cleaning
PN 4237242C
1.
Remove the dust from the upper chassis using a vacuum or brush.
2.
Remove the salt deposits from the bath area and shield.
3.
Inspect the tubing and fitting connections. Remove any salt deposits and repair any leaks
you find.
4.
Bleach the baths and apertures.
5.2-1
5
MAINTENANCE PROCEDURES
PMI RECOMMENDATIONS
Adjustment Procedures
1.
Perform the Latex Gain Adjustment procedure (Heading 4.27).
2.
Perform the AIM Adjustment procedure (Heading 4.28).
3.
Perform the Hgb Preamp Adjustment procedure (Heading 4.29).
Verification Procedures
5.2-2
1.
Perform the REPRODUCIBILITY AND CARRYOVER function found in the Calibration section
of the Operator’s Guide. Both these tests must PASS.
2.
Have the customer run their controls.
PN 4237242C
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CONTENTS
6
SCHEMATICS AND BLOCK DIAGRAMS, 6.1-1
6.1
DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS, 6.1-1
6.2
SCHEMATICS, 6.2-1
TABLES
6.1-1
6.1-2
PN 4237242C
DCNs and Descriptions for Schematics Included in This Manual, 6.1-1
DCNs and Descriptions for Schematics Not Included in This Manual, 6.1-1
6-i
6
CONTENTS
6-ii
PN 4237242C
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6SCHEMATICS AND BLOCK DIAGRAMS 6
6.1
DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS
IMPORTANT Risk of instrument malfunction or erroneous results. Official schematics and drawings in this
section will be revised only with revision of the manual. They will be current on the date of the first printing
of a revision. Reprints between manual revisions will not be updated with current drawing revisions. It is the
user's responsibililty to update these drawings between manual revisions
Table 6.1-1 includes Document Control Numbers (DCNs) and descriptions for the
schematics/diagrams found in Heading 6.2, SCHEMATICS. Table 6.1-2 includes DCNs for
schematics that are available but not included in this manual.
The schematics included in this chapter are updated to the latest revision level whenever this
manual is revised. Because the schematics are not assigned figure numbers or page numbers,
they cannot be included in the table of contents or the index.
Table 6.1-1 DCNs and Descriptions for Schematics Included in This Manual
DCN
Description
6321447
Timing Charts
6321448
Pneumatic/Hydraulic Layout
6321534
Linear Power Supply Card
6321553
AC Power/Vacuum Relay Card
6321564
Motor/Solenoid Driver Card
6321646
Electrical Interconnect Diagram
Table 6.1-2 DCNs and Descriptions for Schematics Not Included in This Manual
PN 4237242C
DCN
Description
6321108
Vacuum Sensor Card
6321634
DRA Card
6321690
Sensor Preamp Adapter Card
6321706
SPAD Card
6321709
Hgb Preamp Card
6321714
URA Card
6.1-1
SCHEMATICS AND BLOCK DIAGRAMS
DOCUMENT CONTROL NUMBERS AND DESCRIPTIONS
6.1-2
PN 4237242C
C
SCHEMATICS AND BLOCK DIAGRAMS
SCHEMATICS
6.2
SCHEMATICS
This section includes the schematics and/or diagrams for the:
PN 4237242C
r
Pneumatic/Hydraulic Layout
r
Timing Charts
r
Electrical Interconnect Diagram
r
Linear Power Supply Card
r
AC Power/Vacuum Relay Card
r
Motor/Solenoid Driver Card.
6.2-1
6
SCHEMATICS AND BLOCK DIAGRAMS
SCHEMATICS
6.2-2
PN 4237242C
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CONTENTS
7
TROUBLESHOOTING, 7.1-1
7.1
TROUBLESHOOTING DIAGNOSTICS, 7.1-1
Customer Options Available for Troubleshooting, 7.1-1
Service Menu, 7.1-2
7.2
GENERATING A SERVICE REPORT, 7.2-1
The Screen Display, 7.2-1
The Printed Report, 7.2-1
AIM Criteria Results, 7.2-2
Hgb Data, 7.2-3
Sample Results - DO NOT REPORT, 7.2-3
7.3
SERVICE DIAGNOSTIC, 7.3-1
7.4
TROUBLESHOOTING ERROR MESSAGES, 7.4-1
7.5
TROUBLESHOOTING POWER-UP PROBLEMS, 7.5-1
ILLUSTRATIONS
7.2-1
7.2-2
7.2-3
Sample Screen Display, 7.2-1
Sample Printed Report, 7.2-2
Hgb Results Analysis, 7.2-4
7.5-1
7.5-2
Power-Up Troubleshooting Flowchart (part 1 of 2), 7.5-1
Power-Up Troubleshooting Flowchart (part 2 of 2), 7.5-2
TABLES
PN 4237242C
7.1-1
7.1-2
Diluter Functions Menu Options, 7.1-1
Service Menu Options, 7.1-2
7.3-1
Service Diagnostic Cycle, 7.3-1
7.4-1
Error Messages, 7.4-1
7-i
7
CONTENTS
7-ii
PN 4237242C
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7TROUBLESHOOTING 7
7.1
TROUBLESHOOTING DIAGNOSTICS
Customer Options Available for Troubleshooting
The Diluter Functions menu provides the customer and Service Representative a means of
basic diluter troubleshooting. There are six functions provided on this menu and they are
described in Table 7.1-1.
Table 7.1-1 Diluter Functions Menu Options
Function
Description
CLEAR
APERTURES
Performs an aperture burn or zap, similar to
the zap performed during an aspiration cycle.
r
As a first attempt at clearing a plugged aperture,
perform several times.
DISPENSE LYSE
Dispenses 415 mL of lyse into the WBC bath.
r
To manually prime the lyse system.
Note: There is no drain function tied to
DISPENSE LYSE, so it is up to the operator to
monitor and drain the WBC bath if necessary.
Note that the lyse sensor (S9) is not used
during this function.
r
To check for bubbles in the lyse system.
r
To verify operation of lyse pump PM5.
r
In the Lyse Volume Adjustment/Verification
procedure (see Heading 4.31).
Drains the baths.
r
Primarily to drain fluid before removing the
baths.
r
To verify the operation of waste pump, PM4, and
solenoids 7 and 10.
r
To verify the operation of rinse pump, PM3, and
solenoid 11.
r
To help detect a plugged 10m filter (FLS2).
r
To check the operation of diluent pump, PM2, if
it is used enough times to force a refill of the
reservoir.
r
To verify the operation of air/mix pump, PM1,
and solenoid 12.
r
To observe plugs or leaks in fluid barrier FLS3
and solenoid 9.
r
To clean the baths with a solution other than
COULTER CLENZ concentrate (cleaning agent).
r
If the CLEAR APERTURES function was
unsuccessful, to add bleach solution as a second
step for attempting to clear a clogged aperture.
DRAIN
RINSE
MIX
CLEAN BATHS
PN 4237242C
Primes the diluent reservoir system and fills
both baths with fresh diluent. First, however,
performs a drain to ensure the baths do not
overflow, eliminating the need to DRAIN the
baths before using RINSE.
Operates by sending mixing bubbles to each
bath in turn.
Drains the baths, then prompts the operator
to add a cleaning solution directly into the
open baths. Use a dilute bleach solution. If
COULTER CLENZ® concentrate is not
available, use COULTER CLENZ cleaning
agent.
When to Use
7.1-1
TROUBLESHOOTING
TROUBLESHOOTING DIAGNOSTICS
Service Menu
The Service menu contains six visible menu items. These are items the customer has access to
but will not often use. They are of most use during troubleshooting and are discussed in
Table 7.1-2. There is a seventh item, Service Diagnostic, that is hidden from the customer.
Table 7.1-2 Service Menu Options
Function
Description
When to Use
LATEX GAIN
(for Coulter
service
personnel only)
Adjusts the RBC and WBC aperture current
settings. Adjustment is necessary when the
mean volumes are greater than 1.5 fL from
the latex assay. See Heading 4.27, LATEX
GAIN ADJUSTMENT.
To adjust the RBC and WBC aperture current settings to
compensate for component and temperature
variations.
REPLACE
SYRINGE
Moves syringes to a middle-stroke position,
making removal/replacement easier.
Each time the syringe bodies are removed.
(for Coulter
service
personnel only)
Note: The power must be turned OFF during
this procedure.
PULSE TEST
Generates a string of pulses, feeds them
into the SPAD card and generates a report
giving counts, MCV and histograms. See
Test Pulse Generator under Heading 2.5,
DATA ACQUISITION.
To verify SPAD card operation when signal processing
of pulse data is in question.
Displays a PASS/FAIL message for the +5 V,
+12 V and +15 V power supplies. Also
displays the aperture current settings and
the Hgb voltage.
To verify the +5 V, +12 V and +15 V power supplies.
Allows the operator to adjust the system
count vacuum.
When the instrument’s count vacuum requires
adjustment.
Note: There is no system indicator for the
high or raw-vacuum output of the vacuum
pump.
To indicate vacuum while troubleshooting.
Determines the software revision level that
is currently loaded by the instrument.
To determine the software revision that is currently
loaded by the instrument.
VOLTAGE
READINGS
VACUUM
ADJUST
REVISION
LEVELS
When the apertures, the Sensor Preamp Adapter card
or the SPAD card are replaced.
Note: This test verifies inoperable or dead circuits on
the SPAD card. It does not however, indicate how well
the SPAD card deals with the complex pulse train of a
real sample.
To verify the aperture current settings.
To troubleshoot/adjust Hgb voltage.
Note: Although there are several hardware
items listed, the hardware cannot indicate a
revision level to the software.
[no menu item
displays on the
screen] Service
Diagnostic
7.1-2
A diluter table (or cycle) that energizes
solenoids and the Probe/Wipe Traverse
Assembly motors, one by one. This function
is password protected and the password is
123. See Heading 7.3, SERVICE
DIAGNOSTIC.
To troubleshoot diluter components.
To verify solenoid, probe motor, traverse motor and
traverse sensor operation.
PN 4237242C
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TROUBLESHOOTING
GENERATING A SERVICE REPORT
7.2
GENERATING A SERVICE REPORT
The Service Report provides data about the previous aspirate cycle, whether it was a sample, a
control or a calibrator. This data is not displayed on any customer screen or printout.
To access the Service Report, at the Main Menu select 5 SPECIAL FUNCTIONS tt 4 SUPERVISOR tt 7
[no menu item displays on the screen].
A partial report with Aperture Integrity Monitor (AIM) data is displayed on the screen, and if
the Auto Print function is active, a more comprehensive report is automatically sent to the
Printer.
The Screen Display
The top line of the display has two data entry points for RBC and WBC 26-percentile target
values (Figure 7.2-1). For a description of what this screen displays, see the information
under the next Heading, The Printed Report.
Figure 7.2-1 Sample Screen Display
7242046A
The Printed Report
If the Service Report function is accessed with Auto Print active, a report is automatically sent
to the printer. An example of the printed report is shown in Figure 7.2-2. The information in
Figure 7.2-2 follows the report, heading by heading, and describes the information contained
in the report.
PN 4237242C
7.2-1
7
TROUBLESHOOTING
GENERATING A SERVICE REPORT
Figure 7.2-2 Sample Printed Report
7242044A
AIM Criteria Results
During data accumulation, pulses are edited so that only good pulses are used to produce
histograms. One output of the Editor circuit is the 26-percentile voltage. This voltage is a
pulse width parameter that is representative of the travel time or flow through the aperture.
An average 26-percentile voltage is measured for each of the 12 count periods that make up
the instrument's data accumulation. This data is used along with the parameter data to
determine abnormal aperture events, specifically clogs or partial clogs. A description of each
line item follows. Specific values are not given for the acceptable ranges since they are subject
to change with software revisions. Use the ranges given on the Service Report of the
instrument you are troubleshooting.
Target (T): The expected 26-percentile voltage for most samples on a given instrument. The
procedure to set target values can be found in Heading 4.28, AIM ADJUSTMENT.
Measured: The average 26-percentile voltage for the entire data accumulation. The acceptable
limits are given on the report as a fraction or percentage of the instrument’s target: example
0.935T is 0.935 times the target value or 6.5% below the target value.
CV: The CV of the 12 count period, 26-percentile results.
Ratio: The ratio of good pulses (or pulses that are accepted by the Editor circuit) to all pulses
counted.
7.2-2
PN 4237242C
TROUBLESHOOTING
GENERATING A SERVICE REPORT
Ratio CV: The ratio of good pulses to total pulses is calculated for each of the 12 count
periods, then the CV of the 12 ratios is calculated.
Voteout: The 12 count periods are grouped into 3 sets of 4 count periods, each set being a
logical aperture. If one "aperture" count is too far removed from the other two, it is "voted
out" and its number is given. If there is no voteout or a total voteout, a zero is given. The
report prints the logical aperture that voted out.
Hgb Data
To help investigate Hgb problems, the Hgb data collected by the instrument during data
accumulation is provided in this section. This data, four readings in all, is the output of the
Hgb Preamp card expressed in mV.
The Hgb parameter is a calculated comparison between a light transmittance reading of the
sample referenced to a reading of clear diluent. Two readings are taken when the sample is in
the bath and two are taken when clear diluent is in the bath. The diluent reading is referred to
as the HGB Blank read, while the sample reading is referred to as the HGB read. Each reading
is actually a series of 50 readings subjected to software filtering and takes about 1 ms to
complete.
The HGB Blank #1 reading is taken on the sample prefill just before the aspirated sample is
introduced to the WBC bath. The HGB Blank # 2 reading is taken on the original rinse just
before the probe moves across to the aspirate position. The two Hgb readings are taken just
before the sample is drained from the WBC bath toward the end of the cycle. There is a
1/2 second delay between the Hgb readings.
Analysis of the Hgb reading results is shown in flowchart form in Figure 7.2-3.
Sample Results - DO NOT REPORT
Several considerations for an AIM determination are based on the parameter results. If any
single AIM criterion fails, the results are displayed with a single asterisk (*) following them. If
however, more than one AIM criterion fails, the results are suppressed and instead a string of
asterisks (*****) is displayed. Since an AIM Alert may suppress the actual results, you must
obtain the Service Report if you need actual results. The actual results are included on the
Service Report for diagnostic use only, and should not be reported.
Below is a listing of AIM criteria based on parameter results.
r
For WBC, ignore CV Ratio if WBC < 2.5 x 103
r
RBC AIM if MCHC < 25 (ignore if Hgb incomplete)
r
RBC AIM if MCHC > 40 (ignore if Hgb incomplete)
r
RBC AIM if (Hgb x 3)/Hct < 0.8 (ignore if Hgb incomplete)
r
RBC AIM if (Hgb x 3)/Hct > 1.2 (ignore if Hgb incomplete)
The instrument does not generate an AIM if:
PN 4237242C
r
Hgb < 1.0 g
r
If Hgb incomplete, RBC < 0.5 x 106
r
For WBC and RBC, a complete voteout occurs
r
For WBC, WBC < 1.0 x 103
7.2-3
7
TROUBLESHOOTING
GENERATING A SERVICE REPORT
Figure 7.2-3 Hgb Results Analysis
IS
BLANK #1 OR
BLANK #2 < 2.5
VOLTS
?
YES
NON-FATAL
ERROR:
Hgb BLANK
VOLTAGE LOW
NO
IS
BLANK 1 OR
BLANK 2 > 4.95
VOLTS
?
YES
NON-FATAL
ERROR:
Hgb BLANK
VOLTAGE HIGH
NO
CALCULATE A
Hgb USING
BOTH BLANK
READINGS
IS RESULT
> 0.6
?
YES
GENERATE
INCOMPLETE
Hgb RESULT
NO
USING HIGHEST
BLANK READING
FOR BOTH, CALCULATE
THE Hgb 1 AND Hgb 2
RESULT
IS Hgb
RESULT
DIFFERENCE
> 0.5
?
YES
GENERATE
INCOMPLETE
Hgb
RESULT
NO
USE Hgb 1
AS RESULT
7.2-4
7242043A
PN 4237242C
C
TROUBLESHOOTING
SERVICE DIAGNOSTIC
7.3
SERVICE DIAGNOSTIC
A Service Diagnostic diluter table (or cycle) has been included in the instrument. The table
energizes solenoids and motors, one by one.
ATTENTION: Once you begin the cycle, you must advance completely through the table.
Pressing the Escape key will not exit the cycle.
To begin the Service Diagnostic, at the Main Menu select 5 SPECIAL FUNCTION tt 5 SERVICE tt 7
[no menu item displays on the screen].
When the instrument prompts you for a password, type 123.
After entering the password, solenoid LV1, the vacuum transducer vent valve located on the
Vacuum Sensor card, is energized. A list of the components and the order in which they are
energized is given in Table 7.3-1. To de-energize the current component and energize the
next, press the Aspirate key.
Table 7.3-1 Service Diagnostic Cycle
PN 4237242C
Sequence
Component
Description - Function
1
LV1
Vents pressure transducer (located on the Vacuum Sensor card).
2
LV2
Vents VIC (located on the Vacuum Sensor card).
3
LV3
Switches between count and high vacuum for VIC.
4
LV4
Opens sweep-flow path.
5
LV5
Opens path between RBC aperture and VIC (count vacuum).
6
LV6
Opens path between WBC aperture and VIC (count vacuum).
7
LV7
Selects which bath to drain.
8
LV8
Opens path from probe wipe to VIC (high vacuum).
9
LV9
Selects LV12 (OFF) or WBC bath air/mix (ON).
10
LV10
Selects bath or VIC to drain.
11
LV11
Selects which bath receives rinse diluent.
12
LV12
Selects RBC bath mix (OFF) or lyse line air gap (ON).
13
LV13
Selects which bath gets prefill.
14
LV14
Switches diluent syringe between bath or probe/probe wipe.
15
LV15
Selects input/output for diluent syringe.
16
LV16
Switches dispense output between probe and probe wipe.
17
Moves sample aspirate probe to the aspirate position.
18
Moves sample aspirate probe to the WBC bath position.
19
Moves sample aspirate probe to the RBC bath position.
20
Moves sample aspirate probe to the down position.
21
Moves sample aspirate probe to the up position.
7.3-1
7
TROUBLESHOOTING
SERVICE DIAGNOSTIC
7.3-2
PN 4237242C
C
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
7.4
TROUBLESHOOTING ERROR MESSAGES
Table 7.4-1 Error Messages
Error Message
Code
Description/Possible Fault
Aspirate Syringe Failure
026
When the syringe is sent to the home position, it stops on sensor. To make
sure it gets to the sensor, it is allowed 40 extra steps. This means that if the
motor is sent down a total of 3000 steps, it is sent up 3040 steps. The sensor
is checked when enough time elapses to complete travel, and if it does not
sense the flag, ERROR DETECTED (026) Aspirate Syringe Failure is displayed
on the screen.
027
A check of the sensor state is made after every downward movement of the
syringe. If the syringe is sensed in the top position, ERROR DETECTED (027)
Aspirate Syringe Failure is displayed on the screen.
018
The hardware for an 8/10 parameter instrument and a 16/18 parameter
instrument are different. If you power up an 8/10 parameter instrument with
16/18 parameter software, the software reverts to the instrument's software
(8/10 parameter).
Copy Protection Violation
If you attempt to use resource files from a 16/18 parameter instrument, on an
8/10 parameter instrument, after power up, ERROR DETECTED (018) Copy
Protection Violation is displayed on the screen.
If this occurs, the files from different instrument type Program Disks were
mixed or a Program Disk from a different instrument type was used after
initial power up.
Count Period Timeout
020
The MD II Series accumulates 12 seconds of count and histogram data for
analysis, collected in 1-second increments. The DMA process used to
channelize histogram data controls the time, but a separate software timer is
used to double check it. If data accumulation is still taking place after 1.2
seconds, ERROR DETECTED (020) Count Period Timeout is displayed on the
screen. The DMA transfer is between the SPAD card and the motherboard.
CPU Fatal Error
013
The CPU on the AT motherboard signals the user software that it has detected
a processor fault, such as an illegal processor instruction or a divide by zero
request. When any error signal is received from the processor, ERROR
DETECTED (013) CPU Fatal Error is displayed on the screen.
This is usually a motherboard problem. However, if the software requests the
CPU to divide by zero, the software is at fault.
Diluent Syringe Failure
PN 4237242C
028
When the syringe is sent to the home position, it stops on sensor. To make
sure it gets to the sensor, it is allowed 40 extra steps. This means that if the
motor is sent down a total of 3000 steps, it is sent up 3040 steps. The sensor
checks when enough time elapses to complete travel, and if it does not sense
the flag, ERROR DETECTED (028) Diluent Syringe Failure is displayed on the
screen.
029
A check of the sensor state is made after every downward movement of the
syringe. If the syringe is sensed in the top position, ERROR DETECTED (029)
Diluent Syringe Failure is displayed on the screen.
7.4-1
7
C
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
Table 7.4-1 Error Messages (Continued)
Error Message
Code
Description/Possible Fault
DRA Board Failure
003
When a stepper motor is set in operation, the DRA card uses a hardware timer
to control the task. When the task is completed, the DRA card generates an
interrupt request signaling that the task is complete. An independent software
timer is allocated whenever a motor is given a task. This timer checks after 30
seconds, and if the interrupt signaling completion of the task has not been
received, ERROR DETECTED (003) DRA Board Failure is displayed on the
screen.
Hgb Blank Voltage High
Non-fatal Two Hgb-blank readings are taken during a sample cycle. If the voltage
Error
received during one of the readings is greater than 4.95 V, Hgb Blank Voltage
High is displayed on the screen.
Hgb Blank Voltage Low
Non-fatal Two Hgb Blank readings are taken during a sample cycle. If the voltage
Error
received during one of the readings is less than 2.5 V, Hgb Blank Voltage Low
is displayed on the screen.
Insufficient RAM
006
The system software dynamically allocates and deallocates memory as
required. If an attempt is made to allocate memory for a task and there is not
enough memory available, ERROR DETECTED (006) Insufficient RAM is
displayed on the screen.
Keypad Failure
010
This error is currently not used by the system. If software is implemented to
check the hardware on the URA card that controls the keypad input, this is the
error code that would be used to indicate a problem.
This error is not called by the software, but its code is in the list of error
messages used when an error occurs. If the message is displayed on the
screen, program execution has lost its way and the error should be treated as
a Software Fatal Error.
Power Supply Failure
7.4-2
014
The MD II has an internal DVM function that does a voltage check of the +15
V, +5 V and +12 V power supplies. This function is called during the self-test
portion of power up and just before results are displayed from a sample,
control, calibration, startup or latex cycle. The DVM function is live, that is, it
reports on the power quality at the time the function is called. It would not
report any failure if the power momentarily dropped out earlier in the cycle. If
a failure is returned by the DVM function call, ERROR DETECTED (014) Power
Supply Failure is displayed on the screen.
015
The MD II does a voltage check of the +24 V power supply at various times.
As with the DVM check, this function is called during the self-test portion of
power up and just before results are displayed from a sample, control,
calibration, startup or latex cycle. It is also called before reporting an
Hgb-blank error, a vacuum error or any sensor error involving a motor. This
includes the two errors for each syringe, the three horizontal traverse position
errors and the two probe vertical position errors. If there is no +24 V, the
motor does not operate, but the problem is not with the motor or sensor
circuit, so this ensures that a misleading error is not generated.
PN 4237242C
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
Table 7.4-1 Error Messages (Continued)
Error Message
Code
Description/Possible Fault
Power Supply Failure
015
The +24 V function checks the state of two latches. One latch is tied to the
POWERFAIL signal from the +24 V power supply, and the other is controlled
by the Overload Timer circuit on the Motor/Solenoid Driver card. If these
latches are set at any time after the last +24 V check, the current +24 V check
generates an ERROR DETECTED (015) Power Supply Failure error message.
For more information about these circuits, see Motor/Solenoid Driver Card
under Heading 2.4, SYSTEM CONTROL.
Probe Mechanism Failure
Probe mechanism movement is controlled by the mechanism stopping at a
sensors. There are five sensors, one at each of the three horizontal positions,
one at the top vertical position and one at the bottom of the vertical position.
Diluter tables send the probe 40 steps more than necessary for horizontal
movement and 15 steps more than necessary for vertical movement. The
probe must travel within 110 steps of its destination or an error is generated.
This description applies to all the Probe Mechanism error codes.
030
When the software is informed that the movement is completed, it checks the
sensor state. If the probe is not at the upper position sensor, or the step
count is too low, ERROR DETECTED (030) Probe Mechanism Failure is
displayed on the screen.
Note: See initial description for more information.
031
When the software is informed that the movement is completed, it checks the
sensor state. If the probe is not at the lower position sensor, or the step count
is too low, ERROR DETECTED (031) Probe Mechanism Failure is displayed on
the screen.
Note: See initial description for more information.
032
When the software is informed that the movement is completed, it checks the
sensor state. If the probe is not at the sensor over the WBC bath, or the step
count is too low, ERROR DETECTED (032) Probe Mechanism Failure is
displayed on the screen.
Note: See initial description for more information.
033
When the software is informed that the movement is completed, it checks the
sensor state. If the probe is not at the sensor over the RBC bath, or the step
count is too low, ERROR DETECTED (033) Probe Mechanism Failure is
displayed on the screen.
Note: See initial description for more information.
034
When the software is informed that the movement is completed, it checks the
sensor state. If the probe is not at the sensor in the aspirate position, or the
step count is too low, ERROR DETECTED (034) Probe Mechanism Failure is
displayed on the screen.
Note: See initial description for more information.
PN 4237242C
7.4-3
7
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
Table 7.4-1 Error Messages (Continued)
Error Message
Code
Description/Possible Fault
RAM Drive Failure
038
During system boot, Virtual RAM (VRAM) drives are created. If the system
was unable to create a drive, ERROR DETECTED (038) RAM Drive Failure is
displayed on the screen.
To create a VRAM drive, you must put the proper line in the CONFIG.SYS file.
This means that the drive is created very early in the boot sequence. High
memory, configured as extended memory, is used for the VRAM drive.
Problems could occur with the disk boot process or the BIOS settings
(especially where RAM is concerned) or with the RAM itself. Assuming a good
disk, the BIOS on the MD II is self-configuring, so the most likely problem is
with RAM.
Software Fatal Error
037
ERROR DETECTED (037) Software Fatal Error is displayed on the screen when
execution of the instrument software has reached an area in the software that
it should never get to. This is in effect, a software safety net.
Software Timer Error
021
At various times during program execution, software timers are allocated to
ensure that events perform as expected. If the system is unable to allocate a
timer, ERROR DETECTED (021) Software Timer Error is displayed on the
screen.
SPAD Board Failure
001
Currently this error is not used by the system.
004
ERROR DETECTED (004) SPAD Board Failure, indicates an overflow of a
channel buffer. The RBC and WBC channeling sections have 16K buffers,
while the Plt uses an 8K buffer. When too many pulses are processed, this
error is displayed on the screen.
039
During an A/D conversion on the SPAD card, a timer checks the time it takes
to complete the conversion. If the conversion does not complete in
approximately 10 µs, ERROR DETECTED (039) SPAD Board Failure is
generated.
005
If the system is unable to load a resource file from a diskette, ERROR
DETECTED (005) System Disk File Corrupt is displayed on the screen.
012
When the system powers up, it searches for a good system configuration
either in CMOS or from the PD.DAT file on the Program Disk and then
attempts to make them the same. ERROR DETECTED (012) System Disk File
Corrupt is displayed on the screen if both configuration sources are bad or if
the system cannot write a new PD.DAT file to disk. See User Resource Adapter
(URA) Card under Heading 2.4, SYSTEM CONTROL for more information
about how CMOS data is handled on power up.
016
ERROR DETECTED (016) System Disk File Corrupt is displayed on the screen
when the system cannot load a Printer report template file from the Program
Disk.
019
The system does a CRC check whenever it loads a file from the floppy drive.
ERROR DETECTED (019) System Disk File Corrupt is generated whenever the
CRC check fails.
System Disk File Corrupt
7.4-4
PN 4237242C
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
Table 7.4-1 Error Messages (Continued)
Error Message
Code
Description/Possible Fault
Unable to Create INF File
Non-fatal Unable to Create INF File is displayed on the screen when the system attempts
Error
to create an INF file and cannot. This message does not stop the system or the
report for the sample being analyzed. It is merely a message for the user's
information.
Creation of the INF file occurs only if SW1-2 of the URA card is ON. This
should only be turned ON if there is a hard drive present for INF files to be
stored on. With no hard drive, INF files are stored to a Virtual RAM drive.
Available memory runs out in about 10 samples and Unable to Create INF File
is generated.
Another reason INF files are not created is that the system date or time is
earlier than the date or time recorded on the last INF file. Unable to Create INF
File is displayed on the screen, allowing you to continue running with no
adverse effects.
Unable to Sense Diluent Level 017
During power up, the diluent reservoir is checked for fluid and for sensor
integrity. The reservoir is drained and overfilled. If the instrument cannot
sense a lack of diluent when drained or the presence of diluent when
overfilled, ERROR DETECTED (017) Unable to Sense Diluent Level is
displayed on the screen.
URA Board Failure
Currently, this error is not used by the system.
002
This error is not called by the software, but its code is in the list of error
messages used when an error occurs. If the message is displayed on the
screen, program execution has lost its way and the error should be treated as
a Software Fatal Error.
008
This error indicates that the software detected a failure of the Analog
Multiplexer circuit on the URA card. The system selects a multiplexer channel,
then the multiplexer is read to see if the channel is selected. If the channel is
not selected, ERROR DETECTED (008) URA Board Failure is displayed on the
screen.
009
The system software uses a timer when it gives a conversion task to the A/D
converter on the URA card. If the conversion does not complete in the allotted
time, ERROR DETECTED (009) URA Board Failure is displayed on the screen.
This A/D converter is used for only one task in the MD II, the DVM check. Any
problem would have to be on the URA card itself.
URA CMOS Failure
011
Vacuum Out of Range
Non-fatal The system count vacuum is recorded during each of the 12 count periods. If
Error
one of these readings is outside of the 5.83 - 6.17 in. Hg limits, Vacuum Out
of Range is displayed on the screen.
PN 4237242C
ERROR DETECTED (011) URA CMOS Failure is displayed on the screen when
the CMOS image is altered after power up. This error is displayed on the
screen whenever the CMOS data is not correct. The power-up routines correct
the CMOS image or generates an ERROR DETECTED (012) System Disk File
Corrupt error message if a correction could not be made.
7.4-5
7
TROUBLESHOOTING
TROUBLESHOOTING ERROR MESSAGES
7.4-6
PN 4237242C
C
TROUBLESHOOTING
TROUBLESHOOTING POWER-UP PROBLEMS
7.5
TROUBLESHOOTING POWER-UP PROBLEMS
The flowchart in Figure 7.5-1can help you troubleshoot when the instrument does not reach
the Main Menu on power up.
Figure 7.5-1 Power-Up Troubleshooting Flowchart (part 1 of 2)
TURN POWER
ON
TURNS ON
LINEAR PS &
SUPPLIES
AC FOR +24 V
+12 V TO K1 ON
AC POWER/
VACUUM
RELAY CARD
POST BEEP
CODES:
APPENDIX TABLE
C.3
+12 V
FAIL
POWER TO
MOTHERBOARD:
CPU BOOTS
CPU
POWER ON
SELF-TEST
+5 V
URA
CARD
+5 V
LCD DISPLAYS
TWO SOLID
BARS
PASS
FAIL
SINGLE BEEP:
END OF POST
BEEP
FLOPPY DRIVE
LED
COMES ON
BOOTS
FROM FLOPPY
DRIVE LOADS 1st OF
2 SYS PROGRAMS
FLOPPY DRIVE
LED MAY
FLASH ON/OFF
PASS
FAIL
LOADS
MAIN SYSTEM
PROGRAM
PASS
PROBLEM WITH
DRIVE, DISKETTE
OR
PROGRAM
FAIL
GOOD LOAD:
MAIN
PROGRAM
RUNS
CLEARS
LCD
LOADS
ERROR AND
MESSAGE
TABLES
COPYRIGHT
MESSAGE
PASS
LOCKS UP IN
CURRENT STATE
PN 4237242C
A
7242047A
7.5-1
7
TROUBLESHOOTING
TROUBLESHOOTING POWER-UP PROBLEMS
Figure 7.5-2 Power-Up Troubleshooting Flowchart (part 2 of 2)
A
DRA1
GENERATES SIGNAL
FOR MOTOR/
SOLENOID
DRIVER CARD
BEGINS
SYSTEM
SELF-TEST
ERROR DETECTED
(015) POWER
SUPPLY FAILURE
FAIL
PERFORMS
+24 V TEST
MOTOR/SOLENOID
DRIVER CARD
GENERATES
POWER ON SIGNAL
TURNS ON
+24 V POWER
SUPPLY
SYSTEM SELF
TEST IN
PROGRESS
FLOPPY DRIVE
LED TURNS OFF
PASS
RUNS
POWER-UP
CYCLE
DISPLAYS
ERROR DETECTED
"POWER
(014) POWER
SUPPLY
SUPPLY FAILURE
FAILURE (014)"
FAIL
PERFORMS
LINEAR PS
(DVM) TEST
PASS
MAIN MENU
7242048A
7.5-2
PN 4237242C
C
CONTENTS
8
PARTS LISTS, 8.1-1
8.1
MASTER PARTS LISTS, 8.1-1
8.2
ILLUSTRATED PARTS, 8.2-1
ILLUSTRATIONS
8.2-1
8.2-2
8.2-3
8.2-4
8.2-5
8.2-6
8.2-7
8.2-8
8.2-9
MD II, Lower Chassis, Left Side View (See Table 8.2-1), 8.2-1
MD II, Front View (See Table 8.2-2), 8.2-2
MD II, Top View into Lower Chassis (See Table 8.2-3), 8.2-3
MD II, Upper Chassis (See Table 8.2-4), 8.2-4
MD II, Back View (See Table 8.2-5), 8.2-5
Traverse Assembly (See Table 8.2-6), 8.2-6
Diluter Assembly (See Table 8.2-6), 8.2-8
Syringe Assembly (See Table 8.2-8), 8.2-10
Peristaltic Pump Assembly (See Table 8.2-9), 8.2-12
TABLES
PN 4237242C
8.1-1
8.1-2
8.1-3
8.1-4
8.1-5
Cables, 8.1-1
Lower Chassis, 8.1-2
Peripherals and Support, 8.1-5
Upper Chassis, 8.1-6
Miscellaneous Hardware, 8.1-7
8.2-1
8.2-2
8.2-3
8.2-4
8.2-5
8.2-6
8.2-7
8.2-8
8.2-9
MD II, Lower Chassis, Left Side View (See Figure 8.2-1), 8.2-1
MD II, Front View (See Figure 8.2-2), 8.2-2
MD II, Top View into Lower Chassis (See Figure 8.2-3), 8.2-3
MD II, Upper Chassis (See Figure 8.2-4), 8.2-4
MD II, Back View (See Figure 8.2-5), 8.2-5
Traverse Assembly (See Figure 8.2-6), 8.2-7
Diluter Assembly (See Figure 8.2-7), 8.2-9
Syringe Assembly (See Figure 8.2-8), 8.2-11
Peristaltic Pump Assembly (See Figure 8.2-9), 8.2-13
8-i
8
CONTENTS
8-ii
PN 4237242C
C
8PARTS LISTS 8
8.1
MASTER PARTS LISTS
These parts lists are in part number order by category. The categories are:
r
Cables
r
Lower Chassis
r
Peripherals and Support
r
Upper Chassis
r
Miscellaneous Hardware.
Table 8.1-1 Cables
Part No.
Description
Figure
Item
6027225-5
Cable, main ac power cable to instrument
8.2-5
10
6027767-2
Cable, power line cord, ac diluter to analyzer
8.2-5
2
6028504-7
Cable, Centronics parallel Printer, 10 ft
8.2-5
1
6028257-9
Cable, RS-232, 25 to 9-pin, used to check Host Interface
not shown
6028265-0
Cable, ground, used on bath and valve shields
not shown
6028337-1
Cable, MSMC and DRA card to Motor/Solenoid Driver card
not shown
6028467-2
Cable, interface between A/B switchbox and MD II, 6 ft
not shown
6028518-7
Cable, ribbon, motherboard to serial 1 connector
not shown
6028522-5
Cable, ribbon, motherboard to parallel connector
not shown
6028597-7
Cable, Probe/Opto card, Flex Connect card
6028668-0
Cable, solenoid extension
6028669-8
Cable, waste pickup tube, level sense
6028672-8
Cable, AIM SPAD card to URA card
PN 4237242C
8.2-6
4
not shown
8.2-1
8
not shown
8.1-1
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-2 Lower Chassis
Part No.
Description
Figure
Item
1017541-1
Reservoir, diluent (uses cover, PN 1019609-4)
8.2-1
1
1019609-4
Cover, for diluent reservoir (PN 1017541-1)
8.2-1
2
1022803-4
Housing, 2-port manifold for diluent syringe, molded
1020601-4
Housing, 3-port manifold for aspirate syringe, molded
8.2-8
10
1021228-6
Fitting, top of aspirate and diluent syringe block
8.2-8
7
1022081-5
Fitting, black plastic feedthrough used on diluter bulkheads
1022814-0
Housing, 2-port manifold for diluent syringe, machined
8.2-8
9
1022827-1
Clip, wire, used to retain probe wipe housing
8.2-6
13
1022895-6
Spool, sweep-flow tubing retainer, molded
8.2-7
4
1022944-8
Spool, machined version of above, use until molded part available
8.2-7
4
1305019-8
Connector, ac input connector and filter
2004013-1
Counter, instrument cycle
2121439-6
Connector, ac cable to upper chassis
2523083-3
Seal, rubber ring, for stepper motor
8.2-9
13
2523618-1
Syringe, glass, 100 µL 1%, aspirate
8.2-2
4
2523628-9
Gear, used on lead screw, syringe and traverse motors
8.2-6
10
8.2-8
2
not shown
not shown
not shown
8.2-2
11
not shown
2523630-1
Belt, syringe lead screw drive
8.2-8
3
2523638-6
Syringe, glass, 5.0 mL 1%, diluent
8.2-2
5
2523793-5
Screw, syringe lead screw and housing assembly
8.2-8
15
2523795-1
Hinge, bath shield
not shown
2523805-2
Hinge, diluent reservoir door
not shown
2523809-5
Belt, traverse horizontal drive
2527674-4
Hinge, left side, panel
2827024-1
8.2-6
3
Screw, 6-32 setscrew for peristaltic pump spool
8.2-9
4
2840053-5
Magnet, diluent reservoir door, catch
8.2-1
3
2840060-8
Plate, diluent reservoir door, magnetic strike
8.2-1
13
2840068-3
Magnet, front door and left side, catch
8.2-1
11
2851121-3
Plate, front door and left side, magnetic strike
8.2-1
14
2851898-6
Foot, rubber
3202220-1
Tubing, sweep-flow (13 ft needed)
3213208-1
Tubing, PharMed
3213214-6
Tubing, silicon, peristaltic pump
8.2-9
6
3230005-7
Tubing, 3-piece, probe wipe (34 in. needed)
8.2-6
20
3814255-1
Filter, Hgb, 525 nm (green), optical
8.1-2
not shown
8.2-7
5
not shown
®
not shown
PN 4237242C
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-2 Lower Chassis (Continued)
Part No.
Description
Figure
Item
4004092-7
Power supply, +24 V switching
8.2-3
1
8.2-5
6
4717896-7
Resistor, 25 W, wire-wound, 15 Ω for motor drivers
8.2-5
7
5102018-9
Fuse, 4 A, 250 V, (F1 and F2 at back, 120 V instrument)
8.2-5
9
5102021-9
Fuse, 2 A, 250 V, (F1 and F2 at back, 220 V instrument)
8.2-5
9
5102084-7
Fuse, 0.4 A, AC Power/Vacuum Relay card, vacuum pump
not shown
6214108-5
Valve, small check
not shown
6232075-3
Valve, 3-way solenoid, air (LV9 and LV12)
8.2-2
10
6232076-1
Filter, green fluid barrier
8.2-1
9
8.2-2
7
8.2-9
5
6232246-2
Fitting, plastic reducer, peristaltic pump tubing
6232259-4
Fitting, 0.093 Y
not shown
6232382-5
Nut, reagent bulkhead Luer fitting
not shown
6232463-5
Fitting, aspirate, front of syringe mount
6232477-5
Fitting, nylon (use on machined baths)
6232483-0
Fitting, syringe block Luer for syringe connection
8.2-8
6
6232497-0
Spacer, red plastic indicator for waste Luer fitting
8.2-1
6
6232498-8
Spacer, white plastic indicator for lyse Luer fitting
8.2-1
4
6232499-6
Spacer, blue plastic indicator for diluent Luer fitting
8.2-1
5
6232502-0
Fitting, tubing Luer for reagent connection
8.2-1
7
6232503-8
Fitting, bulkhead Luer for reagent connection
6232510-1
Fitting, lyse pump, ferrule and threaded nut
8.2-2
9
6232554-2
Filter, fluid, 10 µ, blue
8.2-1
10
6232560-7
Valve, 3-way solenoid, poppet (Vacuum Sensor card)
not shown
6232564-0
Fitting, barbed union, 0.062
not shown
6232628-0
Regulator, vacuum
8.2-2
3
6232718-9
Valve, Angar 2-way solenoid, fluidic
8.2-7
2
6705721-0
Card, Vacuum Sensor
8.2-3
5
6705777-5
Aperture block, RBC
8.2-7
7
6705778-3
Aperture block, WBC
8.2-7
19
6706032-6
Card, Linear Power Supply
8.2-3
2
8.2-5
8
8.2-8
11
not shown
not shown
6706065-2
Card, AC Power/Vacuum Relay
8.2-3
3
6706077-6
Card, Motor/Solenoid Driver
8.2-3
11
6706113-6
Card, Surge/Transient Suppressor
6706150-1
Card, Sensor Preamp Adapter
PN 4237242C
not shown
8.2-3
8
8.1-3
8
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-2 Lower Chassis (Continued)
Part No.
Description
Figure
Item
6706161-6
Card, Probe/Opto Sensor
8.2-6
8
6706166-7
Card, Flex Connect
8.2-3
10
6706202-7
Card, Solenoid Interconnect
8.2-3
9
6706254-0
Sensor, lyse fluid detector assembly
not shown
6706321-0
Guide, traverse housing, lower, machined
not shown
6706322-8
Rack, traverse housing with pin
not shown
6706323-6
Card, Hgb Preamp
8.2-3
7
6805010-3
Sensor, Hgb photodetector
8.2-7
13
6805019-7
Sensor, optical sensor assembly used on traverse
6805022-7
Pump, lyse solenoid
6805024-3
Sensor, optical sensor assembly used on syringe module
6805025-1
Sensor, diluent level sense
8.2-1
12
6805033-2
LED, Hgb
8.2-7
11
6805068-5
Motor, stepper assembly, used throughout instrument
8.2-8
1
8.2-9
2
not shown
8.2-2
8
not shown
6805080-4
Chamber, vacuum isolator (VIC)
8.2-7
3
6805119-3
Motor, probe, vertical position stepper motor assembly
8.2-6
22
6805124-0
Housing, probe wipe, molded
8.2-6
17
6805149-5
Housing, Aperture Electrode module
not shown
6805226-2
Bath, RBC/ WBC assembly, machined
not shown
6805247-5
Bath, RBC/WBC assembly, molded (use when available)
8.2-7
7
6855834-4
Gear, traverse idler pulley
8.2-6
9
6856899-4
Pump, vacuum, 220 V, 50/60 Hz
8.2-3
6
6858007-2
Pump, vacuum, 115 V, 50/60 Hz
8.2-3
6
6859540-1
Transformer, Linear Power Supply card
8.2-3
4
6859596-7
Syringe Assembly
not shown
6859598-3
Probe/Wipe Traverse Assembly
not shown
6859650-5
Spool, peristaltic pump tubing
8.2-9
3
6859707-2
Housing, traverse
8.2-6
11
6859716-1
Housing, probe wipe, machined
8.2-6
17
6859741-2
Probe, aspirate assembly
8.2-6
16
6859786-2
Gear, traverse belt tensioner
8.2-6
37
9921373-6
Holder, fuse, for F1 and F2 at back
8.2-5
9
8.1-4
PN 4237242C
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-3 Peripherals and Support
Part No.
Description
1601018-9
Adhesive, LOCTITE 242, THREADLOCKE2
not shown
1601065-1
Adhesive, LOCTITE 222, THREADLOCKE2
not shown
1604007-0
Lubricant, DOW CORNING 33, silicon grease
not shown
2016511-1
Ribbon, black, for CITIZEN GSX-190 and GSX-200
not shown
2016555-3
Stand, dot matrix Printer
not shown
2016577-4
Switchbox, A/B parallel Printer switcher
not shown
2016583-9
Printer, CITIZEN GSX-190, parallel, 110 V
8.2-5
11
2016584-7
Printer, CITIZEN GSX-190, parallel, 220 V
8.2-5
11
2016671-1
Printer, ticket, Epson TM-290P
8.2-5
5
2016717-3
Ribbon, ink cassette, for Epson TM-290P Printer
not shown
2121864-2
Adapter, ASTM host interface
not shown
3202205-7
Tubing, red stripe for waste output
not shown
3202209-0
Tubing, blue stripe for diluent input
not shown
3202221-9
Tubing, EVA for lyse input
not shown
4004103-6
Power supply, 24V, for Epson TM-290P Printer, Universal
5450104-8
Tool, pin extractor, Mini MATE-N-LOK®
not shown
6415128-2
Software, ASTM Host verification disk, Revision 1.2
not shown
6417372-3
Software, MD II - 8 program disks, English, Revision 1.3
not shown
6417373-1
Software, MD II - 10 program disks, English, Revision 1.3
not shown
6417374-0
Software, MicroDiff II - 16 program disks, English, Revision 1.3
not shown
6417375-8
Software, MicroDiff II - 18 program disks, English, Revision 1.3
not shown
6417384-7
Software, MicroDiff II - 18 program disks, German, Revision 1.3
not shown
6417387-1
Software, MD II - 8 program disks, German, Revision 1.3
not shown
6417388-0
Software, MicroDiff II - 18 program disks, French, Revision 1.3
not shown
6417389-8
Software, MicroDiff II - 16 program disks, French, Revision 1.3
not shown
6417390-1
Software, MD II - 10 program disks, French, Revision 1.3
not shown
6417391-0
Software, MD II - 8 program disks, French, Revision 1.3
not shown
6417392-8
Software, MicroDiff II - 18 program disks, Spanish, Revision 1.3
not shown
6417394-4
Software, MD II - 10 program disks, Spanish, Revision 1.3
not shown
6417395-2
Software, MD II - 8 program disks, Spanish, Revision 1.3
not shown
6417396-1
Software, MicroDiff II - 18 program disks, Japanese, Revision 1.3
not shown
6417399-5
Software, MD II - 8 program disks, Japanese, Revision 1.3
not shown
6417410-0
Software, MicroDiff II - 18 program disks, Italian, Revision 1.3
not shown
6605010-6
Pickup tube, diluent, 10 L
not shown
6605236-2
Pickup tube, lyse
not shown
PN 4237242C
Figure
8.2-5
Item
4
8.1-5
8
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-3 Peripherals and Support (Continued)
Part No.
Description
Figure
Item
6856742-4
Pickup tube, waste
not shown
6857371-8
Particles, latex aperture gain
not shown
6913269-3
Kit, Host Communication, with adapter and manual
not shown
6914956-1
Tool, lyse volume-adjustment measuring vial kit, MD Series
not shown
6915032-2
Software, service replacement Ticket Key Disk Kit
not shown
Table 8.1-4 Upper Chassis
Part No.
Description
1023067-5
Shield, fish paper, for upper chassis card cables
2016503-1
Drive, 3.5 in., 1.44 MB floppy disk
8.2-4
8
2016601-1
Keypad, membrane touch pad (use while available)
8.2-2
2
2016722-0
Keypad, membrane touch pad, tactile response
8.2-2
2
2523685-8
Hinge, upper chassis mounting
8.2-5
3
4004079-0
Power supply, CPU switching
8.2-4
6
6706089-0
Card, DRA (Diluter Resource Adapter)
8.2-4
2
6706165-9
Card, SPAD (Sensor Processing Adapter with Diagnostics)
8.2-4
3
6706170-5
Card, URA (User Resource Adapter)
8.2-4
1
6858009-9
Speaker, CPU subassembly
8.2-4
9
7000156-0
CPU, 386 motherboard
8.2-4
10
7000168-3
Display, LCD main
8.2-2
1
8.1-6
Figure
Item
not shown
PN 4237242C
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-5 Miscellaneous Hardware
Part No.
Description
Figure
Item
1022792-5
Washer, rubber, used to mount old style Hgb LED & photodiode
8.2-7
14
1022916-2
Bushing, front aperture bath clamp
8.2-7
10
1023330-5
Screw, special, used to mount MicroDiff II hardware key
not shown
2121421-3
Contact, socket, MTE
not shown
2121423-0
Connector, 2-pin plug, MTE (Angar solenoid valves)
not shown
2121691-7
Connector, 6-pin, mini univ MATE-N-LOK
2121692-5
Contact, socket, mini univ MATE-N-LOK, 22-18 AWG
not shown
2121718-2
Contact, pin, mini univ MATE-N-LOK, 20-16 AWG
not shown
2121719-1
Contact, socket, mini univ MATE-N-LOK, 26-22 AWG
not shown
2121734-4
Connector, 5-pin plug, MTE
not shown
2121742-5
Connector, 6-pin panel, mini univ MATE-N-LOK
not shown
2121743-3
Connector, 2-pin panel, mini univ MATE-N-LOK
not shown
2121744-1
Connector, 2-pin plug, mini univ MATE-N-LOK
not shown
2121788-3
Contact, pin, mini univ MATE-N-LOK, 26-22 AWG
not shown
2121812-0
Connector, 4-pin plug, mini univ MATE-N-LOK
not shown
2121831-6
Connector, 4-pin panel, mini univ MATE-N-LOK
not shown
2121927-4
Connector, 1-pin plug, mini univ MATE-N-LOK
8.2-7
15
2121928-2
Connector, 1-pin cable, mini univ MATE-N-LOK
8.2-7
17
2512120-1
O-ring, small, aperture/aperture module seal
2523625-4
Bearing, syringe lead screw (used top and bottom)
8.2-8
4
2523644-1
Spring, lead screw tension
8.2-8
13
2523657-2
O-ring, aperture/aperture bath seal
8.2-7
16
2804005-9
Screw, 4-40 x 1/4 in.
8.2-7
12
2806125-1
Screw, 6-32 x 0.38 in. HEX head, used to fasten syringe plunger
2808069-7
Screw, pan head, 8-32 x 0.56 in.
8.2-8
19
2810026-4
Screw, machine, 10-32 x 0.62 in.
8.2-9
8
2810047-7
Screw, 10-32 x 1.5 in. pan head Phillips
8.2-8
22
2822003-1
Nut, HEX, 4-40, used to fasten optical sensor
2822016-2
Nut, #10 HEX
8.2-8
20
2826002-4
Washer, split lock, #4
8.2-7
12
2826035-1
Washer, split lock, #6, used to fasten syringe plunger
2826045-8
Washer, split lock, #10
8.2-9
10
2826048-2
Washer, split lock, #8
8.2-8
18
2827145-0
Washer, flat, #10
8.2-9
9
2827146-8
Washer, flat, #4, used to fasten optical sensor
8.2-7
12
PN 4237242C
8.2-9
12
not shown
not shown
not shown
not shown
8.1-7
8
PARTS LISTS
MASTER PARTS LISTS
Table 8.1-5 Miscellaneous Hardware (Continued)
Part No.
Description
2827147-6
Washer, flat, #6, used to fasten syringe plunger
2827148-4
Washer, flat, #8
8.2-8
17
2851795-5
Screw, captive 6-32 thumbscrew
8.2-2
6
2851835-8
Spacer, #10 St. St. (stainless steel)
8.2-8
21
2851837-4
Spacer, lead screw
8.2-8
12
2851848-0
Nut, 10-32, square
8.2-9
11
2851905-2
Screw, aperture bath mounting thumbscrew
8.2-7
9
2852022-1
Screw, SEMS, pan head Phillips, 4-40 x 1/4 inch
8.2-7
12
2852093-0
Screw, 6-32 x 0.38 in., used to fasten optical sensor bracket
8.2-8
8.2-9
5
7
2852094-8
Screw, SEMS, 6-32 x 0.62 in.
8.2-8
9
6216345-3
Gasket, Hgb LED & Hgb photodiode mounting
not shown
6216357-7
Fitting, sweep-flow
not shown
6805031-6
Bushing, rear aperture module clamp
8.2-7
8
6855211-7
Guide, lead screw guide rod
8.2-8
14
8.1-8
Figure
Item
not shown
PN 4237242C
C
PARTS LISTS
ILLUSTRATED PARTS
8.2
ILLUSTRATED PARTS
Note: na = part number is not available
Figure 8.2-1 MD II, Lower Chassis, Left Side View (See Table 8.2-1)
3
2
1
4
5
6
8
14
13
12
11
10
9
7
Table 8.2-1 MD II, Lower Chassis, Left Side View (See Figure 8.2-1)
Item
Part Number
Description
1
1017541-1
Reservoir, diluent (uses cover PN 1019609-4)
2
1019609-4
Cover, for diluent reservoir (PN 1017541-1)
3
2840053-5
Magnet, diluent reservoir door, catch
4
6232498-8
Spacer, white plastic indicator for lyse Luer fitting
5
6232499-6
Spacer, blue plastic indicator for diluent Luer fitting
6
6232497-0
Spacer, red plastic indicator for waste Luer fitting
7
6232502-0
Fitting, tubing Luer for reagent connection
8
6028669-8
Cable, waste pickup tube, level sense
9
2840068-3
Magnet, front door and left side, catch
10
6232554-2
Filter, fluid, 10 µ, blue
11
6232076-1
Filter, green fluid barrier
12
6805025-1
Sensor, diluent level sense
13
2840060-8
Plate, reservoir door, magnetic strike
14
2851121-3
Plate, left side and front door, magnetic strike
not shown 6232503-8
Fitting, bulkhead Luer, for reagent connection
PN 4237242C
8.2-1
8
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-2 MD II, Front View (See Table 8.2-2)
2
1
7
4
1
_
8
5
2
0
9
6
3
.
3
11
000000
4
10
5
9
8
7
6
7242027B
Table 8.2-2 MD II, Front View (See Figure 8.2-2)
Item
Part Number
Description
1
7000168-3
Display, LCD main
2
2016601-1
Keypad, membrane touch pad (use while available)
2016722-0
Keypad, membrane touch pad, tactile response
3
6232628-0
Regulator, vacuum
4
2523618-1
Syringe, glass, 100 µL 1%, aspirate
5
2523638-6
Syringe, glass, 5.0 mL 1%, diluent
6
2851795-5
Screw, captive 6-32 thumbscrew
7
6232076-1
Filter, green fluid barrier
8
6805022-7
Pump, lyse solenoid
9
6232510-1
Fitting, lyse pump, ferrule and threaded nut
10
6232075-3
Valve, 3-way solenoid, air (LV9 and LV12)
11
2004013-1
Counter, instrument cycle
8.2-2
PN 4237242C
C
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-3 MD II, Top View into Lower Chassis (See Table 8.2-3)
1
2
3
-V4 +
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
A
C P
O
W
ER
/V
AC
U
U
M
V1
+
RTN RTN
+
4
11
MOTOR/SOLENOID DRIVER CARD
5
10
6
9
8
7
7242028A
Table 8.2-3 MD II, Top View into Lower Chassis (See Figure 8.2-3)
Item
Part Number
Description
1
4004092-7
Power supply, +24 V switching
2
6706032-6
Card, Linear Power Supply
3
6706065-2
Card, AC Power/Vacuum Relay
4
6859540-1
Transformer, Linear Power Supply card
5
6705721-0
Card, Vacuum Sensor
6
6856899-4
Pump, vacuum, 220 V, 50/60 Hz
6858007-2
Pump, vacuum, 115 V, 50/60 Hz
7
6706323-6
Card, Hgb Preamp
8
6706150-1
Card, Sensor Preamp Adapter
9
6706202-7
Card, Solenoid Interconnect
10
6706166-7
Card, Flex Connect
11
6706077-6
Card, Motor/Solenoid Driver
PN 4237242C
8.2-3
8
C
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-4 MD II, Upper Chassis (See Table 8.2-4)
2
3
5
4
J15 SER1
6
P8
J17 FLOPPY
J18 PARALLEL
J14 SER2
1
P9
SW1
J20 IDE
J19
EXT BATT
BATTERY
7
BANK 0
BANK 1
BANK 2
BANK 3
W3
10
9
8
7242029A
Table 8.2-4 MD II, Upper Chassis (See Figure 8.2-4)
Item
Part Number
Description
1
6706170-5
Card, URA (User Resource Adapter)
2
6706089-0
Cards, DRA (Diluter Resource Adapter)
3
6706165-9
Card, SPAD (Sensor Processing Adapter with Diagnostics)
4
6028518-7
Cable, motherboard/serial 1 connector, ribbon
5
6028522-5
Cable, motherboard/parallel connector, ribbon
6
4004079-0
Power supply, CPU switching
7
na
Cable, ribbon, floppy disk drive to AT motherboard
8
2016503-1
Drive, 3.5 in., 1.44 MB floppy disk
9
6858009-9
Speaker, CPU subassembly
10
7000156-0
CPU, 386 motherboard
8.2-4
PN 4237242C
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-5 MD II, Back View (See Table 8.2-5)
2
1
3
1
MD II
TICKET
PRINTER
(OPTIONAL)
PRINTER
COMPUTER POWER
4
AC POWER
11
10
9
8
-V3 +
-2V +
+S
-S
ON/OFF
PF/PG
-V4 +
V1
+
RTN RTN
7
+
6
5
7242030B
Table 8.2-5 MD II, Back View (See Figure 8.2-5)
Item
Part Number
Description
1
6028504-7
Cable, Centronics parallel Printer, 10 ft
2
6027767-2
Cable, power line cord, ac diluter to analyzer
3
2523685-8
Hinge, upper chassis mounting
4
4004103-6
Power Supply, 24V, for Epson TM290P Printer, Universal
5
2016671-1
Printer, Ticket, 24 V
4004103-6
Power Supply, Ticket Printer, Universal
2016577-4
Switch Box, A/B Parallel Switch Box
6
4004092-7
Power supply, +24 V switching
7
4717896-7
Resistor, 25 W, wire-wound, 15 Ω for motor drivers
8
6706032-6
Card, Linear Power Supply
9
5102018-9
Fuse, 4 A, 250 V, (F1 and F2 at back, 120 V instrument)
5102021-9
Fuse, 2 A, 250 V, (F1 and F2 at back, 220 V instrument)
9921373-6
Holder, fuse, for F1 and F2 at back
10
6027225-5
Cable, main ac power cable to instrument
11
2016583-9
Printer, CITIZEN GSX-190, parallel, 110 V
2016584-7
Printer, CITIZEN GSX-190, parallel, 220 V
PN 4237242C
8.2-5
8
C
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-6 Traverse Assembly (See Table 8.2-6)
7
8
6
5
1
9
4
37
38
1
39
3
2
1
36
35
10
34
11
33
32
12
13
19
20
18
14
21
6
15
31
22
30
29
16
17
23
28
27
24
26
25
REAR OF TRAVERSE HOUSING
8.2-6
7242031A
PN 4237242C
C
PARTS LISTS
ILLUSTRATED PARTS
Table 8.2-6 Traverse Assembly (See Figure 8.2-6)
Item
1
2
3
4
5
6
7
Part Number
2512104-0
1022783-6
2523809-5
6028597-7
6006044-4
2852218-5
1022774-7
6706322-8
8
6706161-6
9
6855834-4
10
2523628-9
11
6859707-2
12
1022860-3
13
1022827-1
14
2523806-1
15
2512107-4
16
6859741-2
17
6859716-1
6805124-0
18
9908083-3
19
6859756-1
20
3230005-7
21
2852245-9
22
6805119-3
23
6859742-1
24
na
25
2804038-5
26
2806090-4
27
2826035-1
28
2827147-6
29
6805019-7
30
2851356-9
31
2826001-6
32
2802020-1
33
2523815-0
34
6859738-2
35
2808072-7
36
2852100-6
37
6859786-2
38
2827076-3
39
2837031-8
not shown 6805068-5
PN 4237242C
Description
O-ring, flex cable retainer
Shaft, traverse guide
Belt, traverse horizontal drive
Cable, Probe/Opto Sensor card, Flex Connect card
Clamp, aspirate tubing
Screw, SEMS, 4-40 x 3/8 in. long
Rack, traverse
Rack and pin.
Card, Probe/Opto Sensor
Gear, traverse idler pulley
Gear, traverse motor
Housing, traverse
Clamp, clamps 3-tube ribbon to probe housing
Clip, wire, used to retain probe wipe housing
Spring, probe retainer tension spring
O-ring, probe mounting retainer
Probe, aspirate assembly
Housing, probe wipe, machined
Housing, probe wipe, molded
Fitting, union to probe wipe tubing
Clamp, traverse tubing assembly
Tubing, 3-piece, probe wipe (34 in. needed)
Screw, 6-32 special, slotted pan head
Motor, probe, vertical stepper motor assembly
Clamp, traverse drive belt clamp assembly
Guide, traverse housing lower guide (molded)
Screw, flat head, 4-40 x 0.5 in.
Screw, pan head, 6-32 x 0.5 in.
Washer, split lock, #6
Washer, flat, #6
Switch, traverse opto, horizontal position
Washer, flat, #2
Washer, split lock, #2
Screw, pan head, 2-56 x 0.25 in.
Spring, traverse belt tensioner
Bracket, traverse tensioner
Screw, 8-32 x 1.5 in., traverse belt tensioner
Screw, shoulder, tensioner bracket retaining
Gear, traverse belt tensioner
Washer, #4, traverse tensioner gear spacer
Clamp, e-ring, tensioner gear retaining
Motor, stepper assembly, used throughout instrument
8.2-7
8
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-7 Diluter Assembly (See Table 8.2-7)
1
16
17
2
3
19
18
15
14
5
4
13
12
11
10
6
9
8.2-8
8
7
7242032B
PN 4237242C
PARTS LISTS
ILLUSTRATED PARTS
Table 8.2-7 Diluter Assembly (See Figure 8.2-7)
Item
Part Number
Description
1
6232717-1
Valve, Angar 3-way solenoid fluidic
2
6232718-9
Valve, Angar 2-way solenoid fluidic
3
6805080-4
Chamber, vacuum isolator (VIC)
4
1022944-8
Spool, sweep-flow tubing retainer, machined (behind cover plate)
1022895-6
Spool, sweep-flow tubing retainer, molded (behind cover plate)
5
3202220-1
Tubing, sweep-flow (13 ft needed)
6
6705777-5
Aperture block, RBC
7
6805226-2
Bath, aperture, with external electrode connector, machined
6232477-5
Fitting, nylon hose, for machined baths
6805247-5
Bath, aperture, with external electrode connector, molded plasma treated (use when
available)
8
6805031-6
Bushing, rear aperture module clamp
9
2851905-2
Screw, aperture bath mounting thumbscrew
10
1022916-2
Bushing, front aperture bath clamp
11
6805033-2
LED, Hgb
12
2852022
Screw, SEMS, pan head Philiips, Screw, 4-40 x 1/4 in.
13
6805010-3
Sensor, Hgb photodetector
14
1022792-5
Washer, rubber
15
2121927-4
Connector, 1-pin plug, mini univ MATE-N-LOK
16
2523657-2
O-ring, aperture/aperture bath seal
17
2121928-2
Connector, 1-pin cable, mini univ MATE-N-LOK
18
6705778-3
Aperture block, WBC
19
na
Housing, rear Aperture Electrode module
not shown 2512120-1
O-ring, small, aperture/aperture module seal
not shown 3814255-1
Filter, Hgb, 525 nm (green) optical filter
not shown 6216357-7
Fitting, sweep-flow
PN 4237242C
8.2-9
8
C
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-8 Syringe Assembly (See Table 8.2-8)
11
10
9
12
8
7
5
6
5
13
1
14
13
4
15
2
16
3
17
23
22
21
18
19
20
7242033A
8.2-10
PN 4237242C
PARTS LISTS
ILLUSTRATED PARTS
Table 8.2-8 Syringe Assembly (See Figure 8.2-8)
Item
Part Number
Description
1
6805068-5
Motor, stepper assembly
2
2523628-9
Gear, used on lead screw, syringe and traverse motor
3
2523630-1
Belt, syringe lead screw drive
4
2523625-4
Bearing, syringe lead screw (used top and bottom)
5
6805024-3
Sensor, optical assembly used on syringe module
6
2852093-0
Screw, 6-32 x 0.38 in., used to fasten optical sensor bracket
7
6232483-0
Fitting, syringe block Luer for syringe connection
8
1021228-6
Fitting, top of aspirate and diluent syringe block
9
1022814-0
Housing, 2-port manifold for diluent syringe, machined
1022803-4
Housing, 2-port manifold for diluent syringe, molded (right angle)
10
2852094-8
Screw, SEMS, 6-32 x 0.62 in.
11
1020601-4
Housing, 3-port manifold for aspirate syringe, molded
12
6232463-5
Fitting, aspirate fitting, front of syringe mount
13
2851837-4
Spacer, lead screw
14
2523644-1
Spring, lead screw tension
15
6855211-7
Guide, lead screw guide rod
16
2523793-5
Screw, syringe lead screw and housing assembly
17
1020507-7
Bracket, syringe mounting
18
2827148-4
Washer, flat, #8
19
2826048-2
Washer, split lock, #8
20
2808069-7
Screw, pan head, 8-32 x 0.56 in.
21
2822016-2
Nut, #10 HEX
22
2851835-8
Spacer, #10 St. St. (stainless steel)
23
2810047-7
Screw, 10-32 x 1.5 in. pan head Phillips
not shown 2826045-8
Washer, split lock, #10
not shown 2826035-1
Washer, split lock #6, used to fasten syringe plunger
not shown 2827147-6
Washer, flat, #6, used to fasten syringe plunger
not shown 2806125-1
Screw, 6-32 x 0.38 in. HEX head, used to fasten syringe plunger
not shown 2827146-8
Washer, flat, #4, used to fasten optical sensor
not shown 2826002-4
Washer, split lock, #4, used to fasten optical sensor
not shown 2822003-1
Nut, HEX, 4-40, used to fasten optical sensor
PN 4237242C
8.2-11
8
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-9 Peristaltic Pump Assembly (See Table 8.2-9)
1
13
2
12
3
4
11
10
9
8
5
6
7
7242034A
8.2-12
PN 4237242C
PARTS LISTS
ILLUSTRATED PARTS
Table 8.2-9 Peristaltic Pump Assembly (See Figure 8.2-9)
Item
Part Number
Description
1
1014052-8
Washer, stepper motor wear plate
2
6805068-5
Motor, stepper assembly
3
6859650-5
Spool, peristaltic pump tubing
4
2827024-1
Screw, 6-32 HEX head setscrew, peristaltic spool
5
6232246-2
Fitting, plastic reducer, peristaltic pump tubing
6
3213214-6
Tubing, silicon, peristaltic pump
7
2852093-0
Screw, machine, 6-32 x 0.38 in.
8
2810026-4
Screw, machine, 10-32 x 0.62 in.
9
2827145-0
Washer, flat, #10
10
2826045-8
Washer, split-lock, #10
11
2851848-0
Nut, 10-32, square
12
2121691-7
Connector, 6-pin plug, mini univ MATE-N-LOK
13
2523083-3
Seal, rubber ring seal for stepper motor
not shown
1601065-1
Adhesive, LOCTITE 222, THREADLOCKE2
not shown
1604007-0
Lubricant, DOW CORNING 33
not shown
2121719-1
Contact, socket, mini univ MATE-N-LOK, 26-22 AWG
PN 4237242C
8.2-13
8
PARTS LISTS
ILLUSTRATED PARTS
8.2-14
PN 4237242C
C
CONTENTS
A
B
QUICK REFERENCE INFORMATION, A.1-1
A.1
TOLERANCES, VOLTAGES AND LIMITS, A.1-1
Limits, A.1-1
Adjustments, A.1-1
A.2
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS, A.2-1
AC Power/Vacuum Relay Card, A.2-1
Connectors, A.2-1
Test Points, A.2-1
AT Motherboard, A.2-1
DRA Card, A.2-2
Hgb Preamp Card, A.2-2
Test Points, A.2-2
Linear Power Supply Card, A.2-2
Jumpers, A.2-2
Test Points, A.2-2
Motor/Solenoid Driver Card, A.2-3
Jumpers, A.2-3
Test Points, A.2-3
Sensor Preamp Adapter Card, A.2-4
SPAD Card, A.2-4
URA Card, A.2-4
Vacuum Sensor Card, A.2-4
A.3
PRINTER SETTINGS, A.3-1
A.4
FUNCTIONS, A.4-1
Peristaltic Pumps, A.4-1
A.5
MENU TREE, A.5-1
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1
B.1
C
D
MESSAGE/ERROR CODE LISTINGS, C.1-1
C.1
INSTRUMENT NON-FATAL ERROR MESSAGES, C.1-1
C.2
INSTRUMENT FATAL ERROR MESSAGES, C.2-1
C.3
AMIBIOS BEEP CODES, C.3-1
OPTIONAL PRINTERS, D.1-1
D.1
PN 4237242C
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE, B.1-1
EPSON TM-290P SLIP PRINTER, D.1-1
Specifications, D.1-1
Operator Controls and LEDs, D.1-1
DIP Switch SW1 Settings, D.1-2
Installation Procedure, D.1-2
Printer Self-Test, D.1-2
9-i
9
CONTENTS
E
INTELLIGENT SOFTWARE LINK (ISL) OPTION, E.1-1
E.1
ISL OPTION FOR RALS, E.1-1
ISL Description, E.1-1
RALS Installation, Training and Service Responsibilities, E.1-1
Recognizing an MD II Linked to RALS, E.1-1
Servicing an MD II Linked to RALS, E.1-1
ILLUSTRATIONS
A.3-1 CITIZEN GSX-190 Printer Settings, A.3-1
A.5-1 Software Menu Tree, A.5-1
D.1-1 Epson TM-290P Slip Printer Control Locations, D.1-1
TABLES
A.1-1 Calibration Factor Limits, A.1-1
A.1-2 Amplifier Noise Limits, A.1-1
A.1-3 Adjustments, A.1-1
A.2-1
A.2-2
A.2-3
A.2-4
A.2-5
A.2-6
A.2-7
A.2-8
A.2-9
A.2-10
AC Power/Vacuum Relay Card Connectors and Line Input Ranges, A.2-1
AT Motherboard Switch and Jumper Settings, A.2-1
DRA Card Jumper Settings, A.2-2
Linear Power Supply Card Jumper Settings, A.2-2
Linear Power Supply Card Test Points, A.2-2
Motor/Solenoid Driver Card Jumper Settings, A.2-3
Motor/Solenoid Driver Card Test Points, A.2-3
Sensor Preamp Adapter Card Jumper Settings, A.2-4
URA Card Switch and Jumper Settings, A.2-4
Vacuum Sensor Card Test Points, A.2-4
A.4-1 Peristaltic Pumps Location and Function, A.4-1
C.1-1 Non-fatal Error Messages, C.1-1
C.2-1 Fatal Error Messages, C.2-1
C.3-1 AT Motherboard AMIBIOS Beep Codes, C.3-1
D.1-1 DIP Switch SW1 Position Settings, D.1-2
D.1-2 DIP Switch SW1 Settings for International Character Sets, D.1-2
9-ii
PN 4237242C
C
AQUICK REFERENCE INFORMATION A
A.1
TOLERANCES, VOLTAGES AND LIMITS
Limits
Table A.1-1 Calibration Factor Limits
Parameter
Expected Range
WBC
0.975 to 1.169
RBC
1.079 to 1.251
Hgb
0.774 to 0.898
MCV
0.897 to 0.949
Plt
1.004 to 1.214
MPV
1.074 to 1.278
ATTENTION: Millivolts (mV) must be measured with a true rms (root mean square) DMM
(digital multi-meter). The FLUKE® Model 8920A or its equivalent is acceptable.
Table A.1-2 Amplifier Noise Limits
Parameter
Test Points
Expected Range
WBC
TP2, TP15 (ground)
50 to 90 mVrms
RBC
TP3, TP15 (ground)
8 to 15 mVrms
Plt
TP1, TP15 (ground)
40 to 80 mVrms
Adjustments
Table A.1-3 Adjustments
Item
Adjustments
+24 V Power Supply
V1 - This is set at the factory. It cannot and must not be adjusted in the field.
Hgb Preamp Card
SPAD Card
URA Card
Vacuum Sensor Card
PN 4237242C
r
R7 - Hgb preamp gain adjustment (adjusts output voltage)
r
R8 - Preamp offset adjustment (adjusts Hgb zero)
There are two potentiometers onboard, R85 and R86. These are factory
adjustments affecting the Editor circuit that require an oscilloscope and pulse
generator to set them. They cannot and must not be adjusted in the field.
r
R5 - Display contrast
r
R6 - A/D converter zero adjustment
r
R7 - A/D converter scale adjustment
R2 - The Gain adjustment calibrates the card and requires an accurate vacuum
measuring device to set it. It is a factory adjustment and cannot and must not
be adjusted in the field.
A.1-1
C
QUICK REFERENCE INFORMATION
TOLERANCES, VOLTAGES AND LIMITS
A.1-2
PN 4237242C
C
QUICK REFERENCE INFORMATION
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS
A.2
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS
AC Power/Vacuum Relay Card
Connectors
Table A.2-1 AC Power/Vacuum Relay Card Connectors and Line Input Ranges
Connector
Range
100 VOLTS
90 - 110 Vac
120 VOLTS
110 - 132 Vac
220 VOLTS
198 - 242 Vac
240 VOLTS
220 - 264 Vac
Test Points
TP1, TP2-ac in
AT Motherboard
Table A.2-2 AT Motherboard Switch and Jumper Settings
Switch or Jumper
Description
MD II Setting
SW1-1
ON - Uses onboard battery
ON
OFF - Uses off-board battery
SW1-2
ON - Enables battery
ON
OFF - Disables battery
SW1-3
ON - Additional wait states for IDE interface
OFF
OFF - No additional wait states
SW1-4
ON - For color adapter
OFF
OFF - For monochrome adapter (does not matter when using EGA/VGA)
W3 (Jumper)
1 to 2, 3 to 4 - 25K or 1 MB
1 to 2, 3 to 4
2 to 3 - 4 MB
PN 4237242C
A.2-1
A
QUICK REFERENCE INFORMATION
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS
DRA Card
Table A.2-3 DRA Card Jumper Settings
Jumper
Description
MD II Setting
X4
Sets card for IRQ11, needed for DRA1
1-3
Sets card for IRQ12, needed for DRA2
2-3
Sets card to DRA1 or DRA2
ON - DRA1
X5
OFF - DRA2
X6
Connects oscillator to circuit when jumped
ON
Hgb Preamp Card
Test Points
TP1 - orange, output
TP2 - black, ground
Linear Power Supply Card
Jumpers
Table A.2-4 Linear Power Supply Card Jumper Settings
Jumper
Description
MD II Setting
X1
Used to provide grounding for testing the card outside the unit.
OFF
X2
Used to provide grounding for testing the card outside the unit.
OFF
X3
Used to provide grounding for testing the card outside the unit.
OFF
Test Points
Table A.2-5 Linear Power Supply Card Test Points
A.2-2
Test Point
Supply
TP1
+240 Vdc ground
TP2
+240 Vdc
TP3
-15 Vdc ground
TP4
-15 Vdc
TP5
+15 Vdc ground
TP6
+15 Vdc
TP7
Hgb LED cathode (negative lead)
TP8
Hgb LED anode (positive lead)
TP9
RBC aperture voltage
TP10
WBC aperture voltage
TP11
Aperture zap voltage (200 V)
PN 4237242C
QUICK REFERENCE INFORMATION
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS
Motor/Solenoid Driver Card
Jumpers
Table A.2-6 Motor/Solenoid Driver Card Jumper Settings
Jumper
Description
MD II Setting
X1
+24 V control
ON
X2
ON - Connects oscillator to circuit
ON
OFF - Disconnects oscillator for card testing
Test Points
Table A.2-7 Motor/Solenoid Driver Card Test Points
PN 4237242C
Test Point
Supply
TP1
Overload Timer input to comparator
TP2
Overload Timer output
TP3
Common ground
TP4
Lyse sensor output
TP5
Overload timer reference (16 V)
TP6
+24 V supply
TP7
Waste level output
TP8
Probe-wipe down sensor output
TP9
Probe-wipe upper sensor output
TP10
Diluent sensor output
TP11
Spare sensor output
TP12
Probe WBC position sensor output
TP13
Probe aspirate position sensor output
TP14
Probe RBC position sensor output
TP15
Aspirate syringe sensor output
TP16
Diluent syringe sensor output
TP17
POWER ON signal (to +24 V supply)
TP18
Overload Timer +24 V input supply
TP19
Spare sensor output
TP20
Spare sensor output
TP21
Spare sensor output
TP22
Spare sensor output
TP23
Oscillator output
A.2-3
A
QUICK REFERENCE INFORMATION
SWITCH SETTINGS, JUMPER SETTINGS AND TEST POINTS
Sensor Preamp Adapter Card
Table A.2-8 Sensor Preamp Adapter Card Jumper Settings
Jumper
Description
MD II Setting
X3
Grounds WBC Dc Restorer input for subassembly adjustment and testing. OFF
X4
Connects WBC Preamp output to Dc Restorer.
ON
X7
Connects RBC Preamp output to Dc Restorer.
ON
X8
Grounds RBC Dc Restorer input for subassembly adjustment and testing. OFF
SPAD Card
X7 (ON)
r
ON connects oscillator to circuit
r
OFF disconnetcs oscillator for card testing
URA Card
Table A.2-9 URA Card Switch and Jumper Settings
Switch or Jumper
Description
MD II Setting
SW1-1
OFF - MICRO-PAK reagent
OFF
ON - Bulk reagent
SW1-2
OFF - Does not create INF file
OFF
ON - Creates INF file
SW1-3
OFF - Normal operation
OFF
ON - Final test functions
SW1-4
OFF - Normal operation
OFF
ON - Adds Service Report when SW1-3 is ON
SW1-5 to SW1-8
Not used
OFF
X1
Connects oscillator to circuitry
ON
Vacuum Sensor Card
Table A.2-10 Vacuum Sensor Card Test Points
A.2-4
Test Point
Description
TP1
8.006 V reference voltage (7.964 V to 8.049 V)
TP2
Transducer output
TP3
Gain adjustment output
TP4
Ground
TP5
VAC OUT
PN 4237242C
C
QUICK REFERENCE INFORMATION
PRINTER SETTINGS
A.3
PRINTER SETTINGS
Figure A.3-1 shows the MD II settings for the CITIZEN GSX-190 Printer. If that is the Printer
at this site, use these settings.
Figure A.3-1 CITIZEN GSX-190 Printer Settings
PN 4237242C
A.3-1
A
QUICK REFERENCE INFORMATION
PRINTER SETTINGS
A.3-2
PN 4237242C
C
QUICK REFERENCE INFORMATION
FUNCTIONS
A.4
FUNCTIONS
Peristaltic Pumps
Table A.4-1 Peristaltic Pumps Location and Function
PN 4237242C
Pump
Location
Function
PM1 (Air/Mix)
Upper right
Provides air for isolation and mixing bubbles.
PM2 (Diluent)
Upper left
Fills the diluent reservoir from the diluent tube.
PM3 (Rinse)
Lower left
Rinses the baths with fluid from the diluent reservoir.
PM4 (Waste)
Lower right
Drains the VIC and both red and white baths.
A.4-1
A
QUICK REFERENCE INFORMATION
FUNCTIONS
A.4-2
PN 4237242C
C
QUICK REFERENCE INFORMATION
MENU TREE
A.5
MENU TREE
Figure A.5-1 Software Menu Tree
1. RUN SAMPLE
ENTER ID
ENTER RANGE
PASSWORD
CONTROL LEVEL OPTIONS
1. LOW
2. NORMAL
3. HIGH
2. RUN CONTROL
3. STARTUP
ASPIRATE
RUN CONTROL
4. REVIEW LAST SAMPLE
1. SETUP
1. DATE/TIME
2. IQAP ID #
1. SELECT DATE FORMAT
2. CHANGE DATE AND TIME
3. HOST
SETTINGS
1. HOST COMMUNICATION
2. BAUD RATE
3. DATA BITS
4. PARITY
5. STOP BITS
4. OPTIONS
1. PRINT
1. AUTOMATIC
2. MANUAL
2. CONTROL
1. COULTER CONTROL/AUTOMATED DISK
2. OTHER CONTROLS/MANUAL
3. CALIBRATION
1. COULTER CALIBRATOR/AUTOMATED DISK
2. OTHER CALIBRATOR/MANUAL
4. REPORT
FORMAT
1. FULL PAGE REPORT
2. SPLIT PAGE REPORT
3. TICKET REPORT
5. REPORT SECTIONS
1. MICROSCOPIC
2. PATIENT DEMOGRAPHICS
1. PARAMETER UNITS
3. TICKET OPTIONS
2. PARAMETER RANGES
4. HISTOGRAMS
3. PARAMETER LABELS
1. AUTO SEQUENCE ON
2. AUTO SEQUENCE OFF
5. SPECIAL
FUNCTIONS
2. REAGENT
LOT #
6. AUTO SEQUENCE
5. UNITS
3. DILUTER
FUNCTIONS
4. SUPERVISOR
1. STANDARD FORMATS
2. CUSTOM FORMATS
6. INSTITUTION NAME
1. CLEAR APERTURES
2. DISPENSE LYSE
3. DRAIN
4. RINSE
5. MIX
6. CLEAN BATHS
1. QA FUNCTIONS
2. RANGES
3. PASSWORD ACCESS
4. AUTO CALIBRATION
5. PRINT CAL FACTORS
6. CHANGE CAL FACTORS
7. NO ITEM
5. SERVICE
7. NO ITEM
PN 4237242C
1. PRINT SUMMARY
2. PRINT GRAPHS
3. PRINT ASSAY SHEET
4. PRINT ALL QC
1. RANGE 1
2. RANGE 2
3. RANGE 3
1. REPRODUCIBILITY AND CARRYOVER
2. CALIBRATION
3. PRINT CALIBRATION ASSAY SHEET
1. CBC-A
2. CBC-B
3. DIFF
4. ALL
SERVICE REPORT
1. LATEX GAIN (FOR COULTER SERVICE ONLY)
2. REPLACE SYRINGE (FOR COULTER SERVICE ONLY)
3. PULSE TEST
4. VOLTAGE READINGS
5. VACUUM ADJUST
6. REVISION LEVELS
PASSWORD
SERVICE DIAGNOSTIC
PRESENT CONCENTRATED
CLEANER TO THE PROBE
6. SHUTDOWN
7. NO ITEM
1. PRINT QA
2. PREPARE IQAP
3. MEAN TO ASSAY
4. RESTORE DEFAULTS
5. CHANGE ASSAY VALUES
6. CHANGE QC RANGES
1. PRINT RANGES
2. CHANGE RANGES
3. SELECT RANGE FILE(S)
REBOOTS SYSTEM
7242002B
A.5-1
A
C
QUICK REFERENCE INFORMATION
MENU TREE
A.5-2
PN 4237242C
C
BPROCEDURES FOR SPECIAL TOOLS OR SOFTWARE B
B.1
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE
A Service Diagnostic disk will eventually be available for this product. When it becomes
available, this section will contain instructions on its use.
PN 4237242C
B.1-1
C
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE
PROCEDURES FOR SPECIAL TOOLS OR SOFTWARE
B.1-2
PN 4237242C
C
CMESSAGE/ERROR CODE LISTINGS C
C.1
INSTRUMENT NON-FATAL ERROR MESSAGES
Table C.1-1 Non-fatal Error Messages
Displayed Message
Description
Hgb Blank Voltage High
An Hgb-blank reading was greater than 4.95 V.
Hgb Blank Voltage Low
An Hgb-blank reading was less than 2.50 V.
Unable to Create INF File
The system attempted to create an INF file but was unable to do so.
Vacuum out of Range
The vacuum for one of the 12, one-second count periods was outside 5.83 to 6.17 in. Hg.
PN 4237242C
C.1-1
MESSAGE/ERROR CODE LISTINGS
INSTRUMENT NON-FATAL ERROR MESSAGES
C.1-2
PN 4237242C
C
C
MESSAGE/ERROR CODE LISTINGS
INSTRUMENT FATAL ERROR MESSAGES
C.2
INSTRUMENT FATAL ERROR MESSAGES
Table C.2-1 Fatal Error Messages
Error Code
Displayed Message
Description
001
SPAD Board Failure
Currently not used.
002
URA Board Failure
Currently not used.
003
DRA Board Failure
A motor driver did not generate an interrupt to indicate that it finished an
operation.
004
SPAD Board Failure
While channelizing pulses, a buffer overload occurred.
005
System Disk File Corrupt
The system was unable to load a resource file from the Program Disk.
006
Insufficient RAM
The system attempted to allocate memory to perform a function and there
was insufficient memory available.
008
URA Board Failure
The analog multiplexer on the URA card failed.
009
URA Board Failure
There was a timeout while the URA card was performing an A/D conversion.
010
Keypad Failure
Currently not used.
011
URA CMOS Failure
There is a problem with the data stored in the URA CMOS.
012
System Disk File Corrupt
The system could not load an acceptable PD.DAT file from the Program
Disk.
013
CPU Fatal Error
The CPU signaled the system software of a problem. This generally means it
tried to execute an illegal instruction.
014
Power Supply Failure
There was a failure from a voltage check of the +15, +5, +12 and supplies.
015
Power Supply Failure
There was a failure when checking the +24 V power supply.
016
System Disk File Corrupt
The system was unable to load a good printer report template file from the
Program Disk.
017
Unable to Sense Diluent Level During self-test, the system was unable to force a state change of the
diluent sensor.
018
Copy Protection Violation
The instrument detected wrong resource files on the Program Disk.
019
System Disk File Corrupt
The instrument detected a failure of the CRC check while loading a file from
disk. This failure indicates that the system disk file is corrupt.
020
Count Period Timeout
During one of the 12 count periods, the system was channelizing data for
longer than one second.
021
Software Timer Error
The software was unable to allocate a software timer.
026
Aspirate Syringe Failure
The aspirate syringe was not sensed at the home sensor when it should
have been.
027
Aspirate Syringe Failure
The aspirate syringe was sensed at the home sensor when it should not
have been.
028
Diluent Syringe Failure
The diluent syringe was not sensed at the home sensor when it should have
been.
029
Diluent Syringe Failure
The diluent syringe was sensed at the home sensor when it should not have
been.
030
Probe Mechanism Failure
The probe was not at the top position when it should have been.
PN 4237242C
C.2-1
MESSAGE/ERROR CODE LISTINGS
INSTRUMENT FATAL ERROR MESSAGES
Table C.2-1 Fatal Error Messages (Continued)
Error Code
Displayed Message
Description
031
Probe Mechanism Failure
The probe was at the top position when it should not have been.
032
Probe Mechanism Failure
The probe was not above the WBC bath when it should have been.
033
Probe Mechanism Failure
The probe was not above the RBC bath when it should have been.
034
Probe Mechanism Failure
The probe was not at the aspirate station when it should have been.
037
Software Fatal Error
During the execution of the system software, a software error occurred.
038
RAM Drive Failure
The system was unable to create a Virtual RAM drive.
039
SPAD Board Failure
A/D conversion timeout for a SPAD A/D operation.
C.2-2
PN 4237242C
C
C
MESSAGE/ERROR CODE LISTINGS
AMIBIOS BEEP CODES
C.3
AMIBIOS BEEP CODES
Beep codes (Table C.3-1) are errors found during the first stage of the Power On Self Test
(POST). All beep code errors except beep code 8 are fatal errors. A fatal error does not allow
the boot process to continue.
Errors occurring after this phase are non fatal and are displayed to a computer monitor. The
MD II’s display is not a computer monitor and is driven by the system software and hardware.
Since the software is loaded after the boot process is complete, the instrument cannot display
these non-fatal error messages.
Table C.3-1 AT Motherboard AMIBIOS Beep Codes
Beeps
Error Message
Description
1
Refresh Failure
The Memory Refresh circuitry on the motherboard is faulty.
2
Parity Error
The system detected a parity error in base memory.
3
Base 64 KB Memory Failure
The system detected a memory failure in the first 64 KB of memory.
4
Timer Not Operational
The system detected a memory failure in base memory or Timer 1 on the
motherboard is not functioning.
5
Processor Error
The CPU on the motherboard generated an error.
6
8042 - Gate A20 Failure
The CPU is unable to switch to protected mode. Gate A20 on the keyboard
controller (8042) allows the CPU to operate in protected mode.
7
Processor Exception Interrupt
Error
The CPU generated an exception interrupt.
8
Display Memory Read/Write Error
The system video adapter is either missing or its memory is faulty. This is
not a fatal error.
9
ROM Checksum Error
The ROM checksum value does not match the value in BIOS.
10
CMOS Shutdown Register
Read/Write Error
The shutdown register for CMOS RAM has failed.
PN 4237242C
C.3-1
MESSAGE/ERROR CODE LISTINGS
AMIBIOS BEEP CODES
C.3-2
PN 4237242C
C
DOPTIONAL PRINTERS D
D.1
EPSON TM-290P SLIP PRINTER
Specifications
Print method:
Impact dot matrix
Shuttle-type, 7 pin head
Unidirectional printing
We use a 0.63 mm column spacing
We use 4.23 mm (1/6 in.) line spacing
Ribbon:
Exclusive ribbon cassette
Type ERC-27
Purple ink
Life expectancy about 1,500,000 characters
Paper:
Types - Normal (High Quality), pressure sensitive, carbon copy
Total thickness for single-ply paper (no copy) - 0.09 to 0.25 mm
(135 kg paper)
Total thickness with copy paper - 0.09 to 0.35 mm
Maximum copies, 1 original and 2 copies
Interface:
Coulter uses the parallel interface (Centronics compatible)
Operator Controls and LEDs
See Figure D.1-1 for location of operator controls.
RELEASE key:
Releases the paper
REVERSE key:
Feeds the paper backward
FORWARD key:
Feeds the paper forward
POWER LED:
Green - lights when power is on
PAPER OUT LED: Red - lights when paper is out
RELEASE LED:
Green - lights when Printer is in the release state
Green - blinks when Printer is in an error state
Figure D.1-1 Epson TM-290P Slip Printer Control Locations
POWER
RELEASE
PAPER OUT
RESET
FORWARD
REVERSE
RELEASE
7303007A
PN 4237242C
D.1-1
OPTIONAL PRINTERS
EPSON TM-290P SLIP PRINTER
DIP Switch SW1 Settings
Table D.1-1 DIP Switch SW1 Position Settings
Positions/Settings
1 - OFF
2 - ON
3 - ON
4 - ON
ON =
Autofeed
International Character Sets - See
Table D.1-2
5 - OFF
6 - OFF
7 - OFF
8 - OFF
9 - OFF
10 - OFF
Not Used
Not Used
Not Used
Not Used
Not Used
Not Used
Table D.1-2 DIP Switch SW1 Settings for International Character Sets
Position Settings
Country
SW1- 2
SW1- 3
SW1- 4
U.S.A.
ON
ON
ON
France
OFF
ON
ON
Germany
ON
OFF
ON
U.K.
OFF
OFF
ON
Denmark
ON
ON
OFF
Sweden
OFF
ON
OFF
Italy
ON
OFF
OFF
Spain
OFF
OFF
OFF
Installation Procedure
See Epson TM-290P Slip Printer under Heading 3.4, OPTIONAL TICKET PRINTERS.
Printer Self-Test
D.1-2
1.
Ensure the MD II power is on and the Printer power line cord is plugged in.
2.
Press the RELEASE key on the Printer.
3.
Insert a ticket in the Printer.
4.
Unplug the Printer’s power line cord.
5.
Press and hold down the FORWARD key. While holding the FORWARD key, plug in the
power line cord to initiate the self-test.
6.
Press the RESET button to stop the self-test. The self-test stops after printing a specific
number of lines.
PN 4237242C
C
EINTELLIGENT SOFTWARE LINK (ISL) OPTION E
E.1
ISL OPTION FOR RALS
ISL Description
The intelligent software link (ISL) is a software protocol designed to allow a host system to
operate the user interface portion of a Coulter instrument remotely. Since this communication
involves more than a data interface, it is a separate entity from the host computer interface
currently available on most instruments. The ISL is a generic design that is currently used on
the MD II but may in the future be used by different Coulter instruments and with different
host systems.
The initial ISL will be launched in the US only, will be used by MD II Series instruments, and
will be used with Remote Automated Laboratory System (RALS), a laboratory networked
system sold by MAS. Starting with MD II software revision 1.4, the ISL software will be
incorporated in all MD II software.
RALS Installation, Training and Service Responsibilities
Customers will purchase the RALS from MAS. MAS will install, connect, train, and provide
the key disk necessary to enable ISL communication. Coulter Corporation and Coulter
Service will only be responsible for the MD II.
If problems occur with the link, the customer will call MAS first. MAS will check
communication problems and determine whether the RALS is receiving the correct signals
from the MD II. Coulter Service will only be called if MAS determines that the MD II is not
sending the correct signals to the RALS.
Recognizing an MD II Linked to RALS
A key disk is used to enable the ISL option. An MD II that has not had the ISL option enabled
appears and responds just like any other MD II, even if it is linked to a RALS. If the ISL option
has been enabled, but the RALS is not active, the letters "ISL" appear after the software
revision number on the Revision screen (5 SPECIAL FUNCTIONS tt 5 SERVICE tt 6 REVISION).
The RALS is connected to the SERIAL 1 port of the MD II, just like any other host computer
system, but when the RALS is enabled, the SERIAL 1 port is no longer an ASTM Host
Communication port. The Host Communication settings screen applies to the SERIAL 1 port
for setting communication protocols for RALS. When the RALS is connected and active, it
runs the MD II remotely, leaving the LCD screen blank and disabling the keypad. Even
aspiration is initiated at the RALS terminal.
Servicing an MD II Linked to RALS
To service an MD II linked to RALS, you disable the RALS and service the MD II just like an
MD II without a RALS. To disable the RALS, either disconnect the RALS cable from the MD II,
or ask the supervisor of the system to disable the RALS from the terminal.
If you need a Printer for troubleshooting purposes (Service Report), you can connect the
Ticket Printer attached to the RALS terminal to the MD II. You must turn on Auto printing
and select the Ticket Printer option. A ticket key disk is provided with the ISL key disk. MAS
enables the Ticket Printer option when they install the RALS system. Remember to turn off
Auto printing when you have finished servicing the MD II.
PN 4237242C
E.1-1
C
INTELLIGENT SOFTWARE LINK (ISL) OPTION
ISL OPTION FOR RALS
E.1-2
PN 4237242C
INDEX
Symbols
+24 V POWER ON signal
location, 2.2-6
on +24 V power supply, 2.4-9
+24 V power supply
+24 V POWER ON signal, 2.2-6, 2.4-9
adjustments, 2.2-6
description, 2.2-6
ERROR DETECTED (015) Power Supply
Failure, 4.12-3, 7.4-3, C.2-1
ERROR DETECTED (027) Aspirate Syringe
Failure, 7.4-1, C.2-1
fuses used, 2.2-6
installation procedures, 4.12-2
interaction with AC Power/Vacuum Relay
card, 2.2-1
interaction with Power Supply Conditioning
card, 2.5-7
location, 2.2-3, 4.4-1, 4.12-1
location of connections, 4.12-1
part number, 8.1-3, 8.2-3, 8.2-5
POWERFAIL signal, 2.2-6, 2.4-10, 7.4-3
removal procedures, 4.12-1
replacement, 2.2-6
schematics, 2.2-6
table showing adjustments, A.1-1
tools/supplies needed for replacement, 4.12-1
verification procedures, 4.12-3
Numerics
8042 - Gate A20 Failure
description, C.3-1
A
A/D converter
verify operation, 4.8-2
AC Power/Vacuum Relay card
fuses used, 2.2-2, 4.11-1
installation procedures, 4.11-1
interaction with AT power supply, 2.2-2, 2.2-5
interaction with Linear Power Supply card, 2.2-2
jumper, 2.2-5
jumper settings, 3.2-1
location, 2.2-3, 4.4-1
part number, 8.1-3, 8.2-3
removal procedures, 4.11-1
table showing connectors and line input
ranges, A.2-1
PN 4237242
test point and jumper locations, 2.2-3
test point readings, 2.2-5, A.2-1
tools/supplies needed for replacement, 4.11-1
VAC ON signal, 2.2-2, 2.2-5
verification procedures, 4.11-2
Ac Select switch
AT power supply, 2.2-2
switch settings, 3.2-1, 4.5-1
accessibility requirements
description, 3.1-1
accessing the Service Diagnostic
procedures, 4.2-1
adjustments, table of, A.1-1
AIM
criteria results, 7.2-1, 7.2-2
description, 7.2-3
AIM adjustment procedure
PMI recommendations, 5.2-2
steps, 4.28-1
amplifier noise limits
table showing, A.1-1
aperture, bath and Aperture Electrode module
installation procedures, 4.24-2
interaction with Sensor Preamp Adapter
card, 4.24-2
location of components, 4.24-1
removal procedures, 4.24-1
tools/supplies needed for replacement, 4.24-1
verification procedures, 4.24-3
aspiration cycle
table of timing activities, 2.1-5
AT motherboard
AMIBIOS beep codes, C.3-1
description, 2.4-1
ERROR DETECTED (013) CPU Fatal
Error, 7.4-1, C.2-1
installation procedures, 4.6-2
jumper locations, 2.4-1, 4.6-3
jumper settings, 2.4-1, A.2-1
location, 2.2-3, 4.6-1
removal procedures, 4.6-1
System Control, 2.4-1
table of switch and jumper settings, 2.4-1
tools/supplies needed for replacement, 4.6-1
verification procedures, 4.6-3
AT power supply
Ac Select switch, 2.2-2
description, 2.2-1
installation procedures, 4.5-1
location, 2.2-3
INDEX-1
INDEX
Power On/Off switch, 2.2-1, 2.2-2
removal procedures, 4.5-1
replacement, 2.2-1
schematics, 2.2-1
table of output connectors, 2.2-1
tools/supplies needed for replacement, 4.5-1
verification procedures, 4.5-2
AT select switch
switch settings, 3.2-1
attention
definition, 1.1-2
Auto Print function
description, 7.2-1
B
Base 64 KB Memory Failure
description, C.3-1
beep codes
1 Refresh Failure, C.3-1
10 CMOS Shutdown Register Read/Write
Error, C.3-1
2 Parity Error, C.3-1
3 Base 64 KB Memory F, C.3-1
4 Timer Not Operational, C.3-1
5 Processor Error, C.3-1
6 8042 - Gate A20 Failure, C.3-1
7 Processor Exception Interrupt Error, C.3-1
8 Display Memory Read/Write Error, C.3-1
9 ROM Checksum Error, C.3-1
definition, C.3-1
table showing motherboard AMIBIOS beep
codes, C.3-1
C
calibration factor limits
table showing, A.1-1
caution
definition, 1.1-2
circuit cards
AC Power/Vacuum Relay. See AC
Power/Vacuum Relay card
AT motherboard. See AT motherboard
DRA. See DRA card
Flex Connect. See Flex Connect card
Hgb Preamp. See Hgb Preamp card
Linear Power Supply. See Linear Power Supply
card
Motor/Solenoid Driver. See Motor/Solenoid
2-INDEX
Driver card
Probe/Opto Sensor. See Probe/Opto Sensor card
Sensor Preamp Adapter. See Sensor Preamp
Adapter card
Solenoid Interconnect. See Solenoid
Interconnect card
SPAD. See SPAD card
URA. See URA card
Vacuum Sensor. See Vacuum Sensor card
CLEAN BATHS
description, 7.1-1
cleaning
bleach the baths and apertures, 5.2-1
dust, 5.2-1
PMI recommendations, 5.2-1
salt deposits, 5.2-1
CLEAR APERTURES
description, 7.1-1
CMOS RAM
10 CMOS Shutdown Register Read/Write
Error, C.3-1
description, 2.4-3
ERROR DETECTED (006) Insufficient
RAM, 7.4-2, C.2-1
ERROR DETECTED (011) URA CMOS
Failure, 7.4-5, C.2-1
ERROR DETECTED (012) System Disk File
Corrupt, 7.4-4, 7.4-5, C.2-1
errors, 2.4-3
function, 2.4-3
location, 2.4-5
PD.DAT file, 7.4-4
URA card, 2.4-3
verification, 2.4-3
CMOS Shutdown Register Read/Write Error
description, C.3-1
connect the reagents
locations, 3.2-2
procedures, 3.2-2
COULTER MD II Series Analyzer
description, 2.1-1
functional divisions, 2.1-1
operation, 2.1-1
cycle counter
description, 2.3-7
location on Fluidics Panel, 2.3-1
part number, 8.1-2, 8.2-2
PN 4237242
INDEX
D
Data Acquisition section
functional relationship, 2.1-1
Diagnostic Data Acquisition circuit
description, 2.4-4
diluent reservoir
description, 2.3-7
location on Fluidics Panel, 2.3-1
Diluter Functions menu options
CLEAN BATHS, 7.1-1
CLEAR APERTURES, 7.1-1
DISPENSE LYSE, 7.1-1
DRAIN, 7.1-1
MIX, 7.1-1
RINSE, 7.1-1
Diluter Panel
aperture system, 2.3-2
components, 2.3-2
description, 2.3-2
fluidic solenoids, 2.3-1, 2.3-4
Hgb LED, 2.3-2
interaction with Sensor Preamp Adapter
card, 2.3-2
location of components, 2.3-2
location on Fluidics Panel, 2.3-1
parts explosion, 8.2-8
RBC bath, 2.3-2
solenoids, 2.3-1, 2.3-4
sweep-flow system, 2.3-2
VIC, 2.3-2
WBC bath, 2.3-2
Diluter Panel solenoids
installation procedures, 4.25-1
interaction with Solenoid Interconnect
card, 4.25-1
removal procedures, 4.25-1
Service Diagnostic, 4.25-1
tools/supplies needed for replacement, 4.25-1
verification procedures, 4.25-1
Diluter Resource Adapter card. See DRA card
diluter table
aspiration cycle, 2.1-5
function, 2.1-2
power-up cycle, 2.1-6
Service Diagnostic, 7.1-2
timing chart, 2.1-4
troubleshooting using, 2.1-4
DISPENSE LYSE
description, 7.1-1
PN 4237242
display
description, 2.6-1
installation procedures, 4.10-2
interaction with URA card, 2.4-2
location, 2.2-3, 4.10-1
location of connectors, 4.10-1
part number, 8.1-6, 8.2-2
removal procedures, 4.10-1
tools/supplies needed for replacement, 4.10-1
troubleshooting, 2.6-1
verification procedures, 4.10-2
verify operation, 4.8-2
display controller
description, 2.4-3
URA card, 2.4-3
Display Memory Read/Write Error
description, C.3-1
Document Control Numbers (DCN)
for schematics/diagrams included in this
manual, 6.1-1
for schematics/diagrams not included in this
manual, 6.1-1
DRA card
components, 2.4-6
description, 2.4-6
ERROR DETECTED (003) DRA Board
Failure, 7.4-2, C.2-1
installation procedures, 4.9-2
interaction with AT motherboard, 2.4-7
interaction with Motor/Solenoid Driver
card, 2.4-6
jumper locations, 2.4-8
jumper locations and settings, 4.9-1
jumper settings, 4.9-2, A.2-2
location, 2.2-3, 4.6-1, 4.9-2
motor controller, 2.4-6
part number, 8.1-6, 8.2-4
removal procedures, 4.9-1
solenoids, 2.4-6, 2.4-7
stepper motor, 2.4-7
stepper motors, 7.4-2
support circuitry, 2.4-7
System Control, 2.4-1
table of jumper settings, 2.4-8, A.2-2
table of output connectors, 2.4-7
tools/supplies needed for replacement, 4.9-1
verification procedures, 4.9-2
DRAIN
description, 7.1-1
Drain, 2.1-6
INDEX-3
INDEX
E
electronic signals
+24 V POWER ON signal, 2.2-6, 2.4-9
HEMOGLOBIN signal, 2.5-3
HGB VOLTAGE (+5 V), 2.4-4
LYSE PUMP signal, 2.4-9
PLT PREAMP signal, 2.5-9
PLT signal, 2.5-2
POWERFAIL (PF/PG) signal, 2.4-8
POWERFAIL signal, 2.2-6, 2.4-10, 7.4-3
RBC 26-PERCENTILE VOLTAGE, 2.4-4
RBC APERTURE VOLTAGE, 2.4-4
RBC PREAMP signal, 2.5-9
RBC signal, 2.5-2
RBC/PLT APERTURE signal, 2.5-1
VAC ON signal, 2.2-2, 2.2-5
VACUUM READING (+12 V), 2.4-4
VACUUM SENSOR signal, 2.4-5
WBC 26-PERCENTILE VOLTAGE
(+15 V), 2.4-4
WBC APERTURE signal, 2.5-1
WBC APERTURE VOLTAGE, 2.4-4
WBC PREAMP signal, 2.5-9
WBC signal, 2.5-2
electrostatic discharge
warning, 1.2-1
Epson, 3.2-3
ERROR DETECTED (010), 7.4-2
ERROR DETECTED (021), 7.4-4
error messages, 7.4-2, 7.4-4
BIOS settings, 7.4-4
ERROR DETECTED (003) DRA Board
Failure, 7.4-2, C.2-1
ERROR DETECTED (004) SPAD Board
Failure, 7.4-4, C.2-1
ERROR DETECTED (005) System Disk File
Corrupt, 7.4-4, C.2-1
ERROR DETECTED (006) Insufficient
RAM, 7.4-2, C.2-1
ERROR DETECTED (008) URA Board
Failure, 7.4-5, C.2-1
ERROR DETECTED (009) URA Board
Failure, 7.4-5, C.2-1
ERROR DETECTED (011) URA CMOS
Failure, 7.4-5, C.2-1
ERROR DETECTED (012) System Disk File
Corrupt, 7.4-4, 7.4-5, C.2-1
ERROR DETECTED (013) CPU Fatal
Error, 7.4-1, C.2-1
4-INDEX
ERROR DETECTED (014) Power Supply
Failure, 7.4-2, C.2-1
ERROR DETECTED (015) Power Supply
Failure, 4.12-3, 7.4-3, C.2-1
ERROR DETECTED (016) System Disk File
Corrupt, 7.4-4
ERROR DETECTED (017) Unable to Sense
Diluent Level, 2.1-6, 7.4-5, C.2-1
ERROR DETECTED (018) Copy Protection
Violation, 7.4-1, C.2-1
ERROR DETECTED (019) System Disk File
Corrupt, 7.4-4, C.2-1
ERROR DETECTED (020) Count Period
Timeout, 7.4-1, C.2-1
ERROR DETECTED (021) Software Timer
Error, 7.4-4, C.2-1
ERROR DETECTED (026) Aspirate Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (027) Aspirate Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (028) Diluent Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (029) Diluent Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (030) Probe Mechanism
Failure, 7.4-3, C.2-1
ERROR DETECTED (031) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (032) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (033) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (034) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (037) Software Fatal
Error, 7.4-4, C.2-2
ERROR DETECTED (038) RAM Drive
Failure, 7.4-4, C.2-2
ERROR DETECTED (039) SPAD Board
Failure, 7.4-4, C.2-2
Hgb Blank Voltage High, 7.4-2, C.1-1
Hgb Blank Voltage Low, 7.4-2, C.1-1
Software Fatal Error, 7.4-2, 7.4-5
table showing fatal error messages, C.2-1
table showing non-fatal error messages, C.1-1
Unable to Create INF File, C.1-1
Vacuum Out of Range, 7.4-5, C.1-1
PN 4237242
INDEX
F
filters
PMI recommendations, 5.2-1
Flex Connect card
function, 2.3-7
interaction with Probe/Wipe Traverse
Assembly, 2.3-7
LED power indicator, 2.3-7
location, 2.2-3, 4.4-1
part number, 8.1-4, 8.2-3
solenoids, 2.3-7
table of connectors, solenoids, LEDs, 2.3-7
floppy drive
description, 2.6-1
ERROR DETECTED (018) Copy Protection
Violation, 7.4-1, C.2-1
location, 2.2-3
part number, 8.1-6, 8.2-4
verify operation, 4.6-4
fluid reservoir
Fluidics Panel, 2.3-7
fluidic solenoids
Diluter Panel, 2.3-1, 2.3-4
table showing name, type, function, 2.3-4
Fluidics Panel
components, 2.3-1
cycle counter, 2.3-7
fluid reservoir, 2.3-7
function, 2.3-1
internal seals, 2.3-8
location, 2.3-1
location of components, 2.3-1
lyse pump, 2.3-8
vacuum regulator, 2.3-9
Fluidics Panel section
functional relationship, 2.1-1
fuses
on +24 V power supply, 2.2-6
on AC Power/Vacuum Relay card, 2.2-2
part numbers, 8.1-3, 8.2-5
H
HEMOGLOBIN signal
Hgb Preamp card, 2.5-3
Hgb
sample analysis of results, 7.2-3
Hgb Blank Voltage High
description, C.1-1
PN 4237242
Hgb Blank Voltage Low
description, C.1-1
Hgb Preamp adjustment procedure
PMI recommendations, 5.2-2
steps, 4.29-1
tools/supplies needed for replacement, 4.29-1
Hgb Preamp card
adjustments, 2.5-3, 2.5-4
description, 2.5-3
HEMOGLOBIN signal, 2.5-3
location, 2.2-3, 4.4-1
part number, 8.1-4, 8.2-3
table showing adjustments, A.1-1
test points, 2.5-4, A.2-2
VOLTAGE READINGS, 7.1-2
hidden menu items for service
location, 2.1-2
I
important
definition, 1.1-2
initial setup of instrument
calibrate the instrument, 3.3-1
connect the Printer, 3.2-3
peristaltic pump tubing, 3.2-1
startup the instrument, 3.3-1
unpack the instrument, 3.2-1
installation procedures
instrument, 3.2-1
optional Printers, 3.4-1
instrument cycling
description, 2.1-2
intelligent software link (ISL) option
description, E.1-1
J
jumper settings
AC Power/Vacuum Relay
card, 2.2-5, 3.1-2, 3.2-1, A.2-1
AT motherboard, 2.4-1, A.2-1
DRA card, 2.4-8, A.2-2
Linear Power Supply card, 2.2-8, A.2-2
Motor/Solenoid Driver card, 2.4-11, A.2-3
Sensor Preamp Adapter card, 2.5-2, A.2-4
URA card, 2.4-6, A.2-4
INDEX-5
INDEX
K
keypad
8279 keyboard controller chip, 2.4-3
description, 2.6-1
input connector, 2.4-5
installation procedures, 4.10-2
interaction with URA card, 2.4-2
location, 2.2-3, 4.10-1
location of connectors, 4.10-1
part number, 8.1-6, 8.2-2
removal procedures, 4.10-1
replacement, 2.6-1
Software Fatal Error, 7.4-2, 7.4-4
tools/supplies needed for replacement, 4.10-1
verification procedures, 4.10-2
verify operation, 4.8-2
keypad controller
description, 2.4-3
Keypad Failure, 7.4-2
L
LATEX GAIN
description, 7.1-2
latex gain adjustment procedure
Linear Power Supply card, 2.2-7
PMI recommendations, 5.2-2
LEDs
Hgb LED, 2.2-8, 2.3-2
Hgb LED current, 2.2-1, 4.13-3
Motor/Solenoid Driver card, 2.4-9
on Flex Connect card, 2.3-7
Solenoid Interconnect card, 2.3-8
limits
amplifier noise, table of, A.1-1
calibration factors, table of, A.1-1
Linear Power Supply card
description, 2.2-6
Hgb LED, 2.2-8
installation procedures, 4.13-2
interaction with Sensor Preamp Adapter
card, 4.13-3
interaction with SPAD card, 2.2-7
jumper locations and settings, 2.2-7, 4.13-1
jumper settings, 2.2-8, 4.13-2
latex gain adjustment procedure, 2.2-7
LED locations, 2.2-7
location, 2.2-3, 4.4-1, 4.13-2
location of test points and jumpers, 4.13-2
6-INDEX
part number, 8.1-3, 8.2-3, 8.2-5
removal procedures, 4.13-1
table of test points, 2.2-8, A.2-2
table showing jumper settings, A.2-2
test point locations, 2.2-7, 4.13-1
tools/supplies needed, 4.13-1
verification procedures, 4.13-3
verify voltages, 4.11-2
lower chassis
opening, 2.2-3
lyse pump
description, 2.3-8
Fluidics Panel, 2.3-8
location on Fluidics Panel, 2.3-1
Motor/Solenoid Driver card, 2.4-9
LYSE PUMP signal
function, 2.4-9
lyse system
troubleshooting, 2.3-8
lyse volume adjustment/verification procedure
tools/supplies needed, 4.31-1
when to perform, 4.31-1
M
manual
conventions, 1.1-2
organization, 1.1-1
scope, 1.1-1
special headings, 1.1-1
manuals, customer
part numbers, 1.1-1
MIX
description, 7.1-1
Motor
Motor/Solenoid Driver card, 2.4-9
Motor/Solenoid Driver card
description, 2.4-8
installation procedures, 4.15-1
interaction with DRA cards, 2.4-8, 2.4-9
jumper locations and settings, 4.15-2
LED, 2.4-9
location, 2.2-3, 4.4-1
lyse pump, 2.4-9
part number, 8.1-3, 8.2-3
POWERFAIL signal, 2.4-10
removal procedures, 4.15-1
Service Diagnostic, 4.15-3
solenoid driver, 2.4-9
System Control, 2.4-1
PN 4237242
INDEX
table of input connectors, 2.4-10
table of jumper settings, 2.4-11, A.2-3
table of output connectors, 2.4-10
table of test points, 2.4-11, A.2-3
table showing jumper settings, A.2-3
tools/supplies needed, 4.15-1
unpacking the instrument, 3.2-1
verification procedures, 4.15-2
motors
DRA card, 2.4-6, 2.4-7
interaction with DRA card, 7.4-2
Motor/Solenoid Driver card, 2.4-9
peristaltic pump motors, 4.22-2
probe motor, 2.3-4, 2.4-9
stepper motor, 2.3-4, 2.3-6, 2.4-6, 2.4-7
Syringe Assembly, 4.21-3
traverse motor, 2.3-4, 2.4-10, 4.20-1, 4.20-2
N
note
definition, 1.1-2
O
octal DAC
description, 2.5-8
opening the lower chassis
procedures, 4.4-1
optional ticket printer
installation, 3.4-1
locations of connections, 3.2-3
Oscillator circuit
description, 2.5-7
SPAD card, 2.5-7
P
Parity Error
description, C.3-1
part numbers
cables, 8.1-1
customer manuals, 1.1-1
fuses, 8.1-3
lower chassis items, 8.1-2, 8.1-3, 8.1-4
miscellaneous hardware items, 8.1-7, 8.1-8
peripherals and support items, 8.1-5
upper chassis items, 8.1-6
PD.DAT file
CMOS RAM, 2.4-3
PN 4237242
errors, 2.4-3
peripherals
display, 2.6-1
floppy drive, 2.6-1
keypad, 2.6-1
Peripherals section
functional relationship, 2.1-1
Peristaltic Pump Assembly
components, 2.3-5
description, 2.3-5
location on Fluidics Panel, 2.3-1
parts explosion, 8.2-12
peristaltic pump motors, 4.22-2
table showing location and function, 2.3-6
peristaltic pump motor and spool
installation procedures, 4.22-2
location, 4.22-1
location of components, 4.22-1
removal procedures, 4.22-1
tools/supplies needed for replacement, 4.22-1
verification procedures, 4.22-2
peristaltic pump tubing
PMI recommendations, 5.2-1
peristaltic pumps
on Fluidics Panel, 2.3-1
table showing location and
function, 2.3-6, A.4-1
verify operation, 4.9-2, 4.15-3, 4.22-2
PLT PREAMP signal
table showing SPAD card connections, 2.5-9
PLT signal
table showing Sensor Preamp Adapter card
connections, 2.5-2
PMI recommendations
AIM Adjustment procedure, 5.2-2
cleaning, 5.2-1
filters, 5.2-1
Hgb preamp adjustment procedure, 5.2-2
latex gain adjustment procedure, 5.2-2
peristaltic pump tubing, 5.2-1
polyurethane tubing, 5.2-1
syringes, 5.2-1
verification procedures, 5.2-2
polyurethane tubing
PMI recommendations, 5.2-1
positioning, 7.4-3
power, 2.1-6
power ranges, 3.1-1
table showing connector and power
ranges, A.2-1
INDEX-7
INDEX
table showing connector, power ranges, part
numbers, 3.1-2
power supplies
+24 V power supply, 2.2-6
AT power supply, 2.2-1
description, 2.2-1
ERROR DETECTED (014) Power Supply
Failure, 7.4-2, C.2-1
ERROR DETECTED (015) Power Supply
Failure, 4.12-3, 7.4-3, C.2-1
fuses used, 2.2-1
input/output voltage, 2.2-1
interaction with DRA card, 2.2-1
interaction with Linear Power Supply card, 2.2-1
interaction with Motor/Solenoid Driver
card, 2.2-1
Linear Power Supply card, 2.2-6
Power Supply Conditioning card
description, 2.5-7
interaction with +24 V power supply, 2.5-7
Power Supply section
functional relationship, 2.1-1
POWERFAIL signal, 2.4-8
interaction with Motor/Solenoid Driver
card, 2.4-10
location, 2.2-6
on +24 V power supply, 2.4-10
power-up cycle
table of timing activities, 2.1-5
preinstallation checks
accessibility requirements, 3.1-1
power requirements, 3.1-1
space requirements, 3.1-1
table showing space requirements, 3.1-1
Printer
CITIZEN GSX-190 part number, 8.1-5, 8.2-5
CITIZEN GSX-190 settings, 3.2-4, A.3-1
connecting, 3.2-3
Epson TM-290P Slip Printer DIP switch
settings, D.1-2
Epson TM-290P Slip Printer operator
controls, D.1-1
Epson TM-290P Slip Printer self test, D.1-2
Epson TM-290P Slip Printer
specifications, D.1-1
ERROR DETECTED (017) Unable to Sense
Diluent Level, C.2-1
ERROR DETECTED (019) System Disk File
Corrupt, C.2-1
8-INDEX
installing Graphic only, 3.2-3
installing Ticket and Graphic, 3.4-3
installing Ticket only, 3.4-1
locations of connections, 3.2-3
verify operation of port, 4.6-4
probe and probe wipe
installation procedures, 4.17-2
location of components, 4.17-1
removal procedures, 4.17-1
tools/supplies needed for replacement, 4.17-1
verification procedures, 4.17-3
probe motor, 7.4-3
installation procedures, 4.18-1
interaction with Probe/Opto Sensor card, 4.18-1
Motor/Solenoid Driver card, 2.4-9
Probe/Wipe Traverse Assembly, 2.3-4
removal procedures, 4.18-1
tools/supplies needed for replacement, 4.18-1
verification procedure, 4.18-1
Probe/Opto Sensor card
interaction with probe motor, 4.18-1
part number, 8.1-4, 8.2-7
Probe/Wipe Traverse Assembly
adjustments, 2.3-4
components, 2.3-4
ERROR DETECTED (030) Probe Mechanism
Failure, 7.4-3, C.2-1
ERROR DETECTED (031) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (032) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (033) Probe Mechanism
Failure, 7.4-3, C.2-2
ERROR DETECTED (034) Probe Mechanism
Failure, 7.4-3, C.2-2
function, 2.3-1, 2.3-4
interaction with Flex Connect card, 2.3-7
location of components, 2.3-4
location on Fluidics Panel, 2.3-1
part number, 8.1-4
parts explosion, 8.2-6
probe motor, 2.3-4
traverse motor, 2.3-4
procedures
accessing hidden service menu items, 4.2-1
accessing the Service Disgnostic, 4.2-1
accessing the Service Report, 4.2-1
AIM adjustment, 4.28-1
calibration, 3.3-1
PN 4237242
INDEX
connecting the Printer, 3.2-3
connecting the reagents, 3.2-2
entering the initial instrument settings, 3.2-4
Hgb preamp adjustment, 4.29-1
latex gain adjustment, 2.2-7, 4.27-1, 7.1-2
lyse volume adjustment/verification, 7.1-1
opening the lower chassis, 4.4-1
rebooting the system, 4.2-2
removing the top cover, 4.3-1
Service Diagnostic, 7.3-1
starting the instrument, 3.3-1
system verification, 5.1-1
unpacking the instrument, 3.2-1
vacuum adjustment, 4.14-2, 4.23-1, 4.30-1
Processor Error
description, C.3-1
Processor Exception Interrupt Error
description, C.3-1
PULSE TEST
description, 7.1-2
Service menu options, 7.1-2
SPAD card, 4.7-2
pumps
lyse pump, 2.3-8, 2.4-9
peristaltic pumps, 2.3-1, 2.3-5, A.4-1
vacuum pump, 2.2-5
pumps/peristaltic pumps, 2.3-6
R
RBC bath
activity on power-up cycle, 2.1-6
description, 2.3-2
RBC PREAMP signal
table showing SPAD card connections, 2.5-9
RBC signal
table showing Sensor Preamp Adapter card
connections, 2.5-2
RBC/PLT APERTURE signal
table showing Sensor Preamp Adapter card
connections, 2.5-1
rear panel interface connectors
parallel printer connector, 2.6-1
Serial 1 connector, 2.6-1
Serial 2 connector, 2.6-1
rebooting the system
procedures, 4.2-2
Refresh Failure
description, C.3-1
Remote Automated Laboratory System (RALS)
PN 4237242
servicing MD II with, E.1-1
removal/installation/verification procedures
guidelines, 4.1-1
removing the top cover
procedures, 4.3-1
REPLACE SYRINGE
description, 7.1-2
reproducibility and carryover
PMI recommendations, 5.2-2
System Verification Procedure, 5.2-2
REVISION LEVELS
description, 7.1-2
RINSE
description, 7.1-1
ROM Checksum Error
description, C.3-1
S
safety precautions
biological, 1.2-1
electronic, 1.2-1
troubleshooting, 1.2-2
schematics/diagrams
table showing those included in this
manual, 6.1-1
table showing those not included in this
manual, 6.1-1
Sensor Preamp Adapter card
adjustments, 2.5-2
description, 2.5-1
installation procedures, 4.16-2
interaction with Diluter Panel, 2.3-2
interaction with SPAD card, 2.5-1
jumper locations and settings, 2.5-2, 4.16-3
jumper settings, 4.16-2
location, 2.2-3, 4.4-1, 4.16-1
part number, 8.1-3, 8.2-3
PULSE TEST, 7.1-2
removal procedures, 4.16-1
table of input connectors, 2.5-1
table of jumper settings, 2.5-2, A.2-4
table of output connectors, 2.5-2
tools/supplies needed for replacement, 4.16-1
verification procedures, 4.16-3
Sensor Processing Adapter with Diagnostics card.
See SPAD card
Serial 1 connector
function, 2.6-1
table of pinouts, 2.6-1
INDEX-9
INDEX
Serial 2 connector
function, 2.6-1
table of pinouts, 2.6-1
serial ports
Serial 1, 2.6-1
Serial 2, 2.6-1
table of pinouts, 2.6-1
verify operation of port, 4.6-4
Service Diagnostic
description, 7.1-2, 7.3-1
Diluter Panel solenoids, 4.25-1
diluter table, 2.1-2
interaction with Vacuum Sensor card, 7.3-1
Motor/Solenoid Driver card, 4.15-3
procedures, 7.3-1
table showing cycle, 7.3-1
verify operation of solenoid valves, 4.9-2
Service menu options
LATEX GAIN, 7.1-2
PULSE TEST, 7.1-2
REPLACE SYRINGE, 7.1-2
REVISION LEVELS, 7.1-2
Service Diagnostic, 7.1-2
VACUUM ADJUST, 7.1-2
VOLTAGE READINGS, 7.1-2
Service Report
procedures to generate, 7.2-1
sample Service Report, 7.2-1
software menu system
description, 2.1-2
hidden menu items for service, 2.1-2
software menu tree, 2.1-2
software menu tree
illustration, 2.1-2, A.5-1
software tables
diluter table, 2.1-2
function, 2.1-2
instrument cycling, 2.1-2
Software Timer Error, 7.4-4
solenoid controller
DRA card, 2.4-7
Solenoid Interconnect card
description, 2.3-8
location, 2.2-3, 4.4-1
part number, 8.1-4, 8.2-3
table of connectors, solenoids, LEDs, 2.3-8
solenoid valves
Vacuum Sensor card, 2.5-4, 2.5-5
solenoids
10-INDEX
Diluter Panel, 2.3-2
DRA card, 2.4-6, 2.4-7
fluidic solenoids, 2.3-1, 2.3-4
Motor/Solenoid Driver card, 2.4-9
on Flex Connect card, 2.3-7
space requirements
description, 3.1-1
table showing space requirements, 3.1-1
SPAD card
aperture signal processing, 2.5-8
control functions, 2.5-7
control signals/commands, 2.5-7
description, 2.5-6
ERROR DETECTED (004) SPAD Board
Failure, 7.4-4, C.2-1
ERROR DETECTED (039) SPAD Board
Failure, 7.4-4, C.2-2
input connector, 2.5-9
installation procedures, 4.7-1
interaction with AT motherboard, 2.5-7
interaction with Linear Power Supply card, 2.5-7
interaction with Sensor Preamp Adapter
card, 2.5-6
interrupt selector, 2.5-9
jumper location and setting, 2.5-9
jumper setting, 4.7-1
jumper settings, A.2-4
location, 2.2-3, 4.6-1, 4.7-1
location of connectors, 2.5-9
octal DAC, 2.5-8
Oscillator circuit, 2.5-7
part number, 8.1-6, 8.2-4
power supply conditioning, 2.5-7
Power Supply Conditioning card, 2.5-7
PULSE TEST, 4.7-2, 7.1-2
removal procedures, 4.7-1
table showing adjustments, A.1-1
test pulse generator, 2.5-9
tools/supplies needed for replacement, 4.7-1
verification procedures, 4.7-2
special headings, 1.1-1
attention, 1.1-2
caution, 1.1-2
important, 1.1-2
note, 1.1-2
warning, 1.1-1
stepper motor, 7.4-2
DRA card, 2.4-6
location on Syringe Assembly, 2.3-6
PN 4237242
INDEX
SVP, 5.1-1
sweep-flow system
Diluter Panel, 2.3-2
sweep-flow tubing
installation procedures, 4.26-2
interaction with Diluter Panel, 4.26-1
location, 4.26-1
location of components, 4.26-1
part number, 4.26-2
removal procedures, 4.26-1
tools/supplies needed for replacement, 4.26-1
verification procedures, 4.26-2
switch settings
Ac Select switch, 3.2-1
AT motherboard, 2.4-1, A.2-1
URA card, 2.4-6, A.2-4
switches
Ac Select switch, 2.2-2
instrument’s power ON/OFF
switch, 2.2-1, 2.2-2
Syringe Assembly
components, 2.3-6
description, 2.3-6
ERROR DETECTED (026) Aspirate Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (027) Aspirate Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (028) Diluent Syringe
Failure, 7.4-1, C.2-1
ERROR DETECTED (029) Diluent Syringe
Failure, 7.4-1, C.2-1
installation procedures, 4.21-3
location of components, 2.3-6
location on Fluidics Panel, 2.3-1
part number, 8.1-4
parts explosion, 8.2-10
removal procedures, 4.21-1
stepper motor, 2.3-6
tools/supplies needed for replacement, 4.21-1
verification procedures, 4.21-4
syringes
PMI recommendations, 5.2-1
System Control section
AT motherboard, 2.4-1
components, 2.4-1
DRA card, 2.4-1
functional relationship, 2.1-1
Motor/Solenoid Driver card, 2.4-8
URA card, 2.4-1, 2.4-2
system verification procedure, 5.1-1
PN 4237242
T
Timer Not Operational
description, C.3-1
timing chart
diluter table, 2.1-4
for troubleshooting, 2.1-4
top cover
removing, 2.2-3
traverse drive belt
installation procedures, 4.19-2
removal procedures, 4.19-1
tools/supplies needed, 4.19-1
verification procedures, 4.19-3
traverse motor
installation procedures, 4.20-2
location of components, 4.20-1
Motor/Solenoid Driver card, 2.4-9
Probe/Wipe Traverse Assembly, 2.3-4
removal procedures, 4.20-1
tools/supplies needed for replacement, 4.20-1
verification procedures, 4.20-3
troubleshooting
display, 2.6-1
generating a Service Report, 7.2-1
lyse system, 2.3-8
safety precautions, 1.2-2
sample screen display, 7.2-1
sample Service Report, 7.2-1
Service Diagnostic, 7.3-1
table showing Diluter Functions menu
options, 7.1-1
table showing Service Diagnostic cycle, 7.3-1
table showing Service menu options, 7.1-2
using timing chart, 2.1-4
U
Unable to Create INF File, 7.4-5
description, C.1-1
URA card
adjustments, 2.4-5
CMOS RAM, 2.4-3, 7.4-4
components, 2.4-2
description, 2.4-2
Diagnostic Data Acquisition circuit, 2.4-4
display, 2.4-2
display controller, 2.4-3
ERROR DETECTED (008) URA Board
Failure, 7.4-5, C.2-1
INDEX-11
INDEX
ERROR DETECTED (009) URA Board
Failure, 7.4-5, C.2-1
ERROR DETECTED (011) URA CMOS
Failure, 7.4-5, C.2-1
ERROR DETECTED (012) System Disk File
Corrupt, 7.4-5, C.2-1
ERROR DETECTED (016) System Disk File
Corrupt, 7.4-4
input/output voltage, 2.4-4
installation procedures, 4.8-2
jumper and switch settings, 4.8-2
jumper location and setting, 2.4-5, 4.9-1
keypad controller, 2.4-4
location, 2.2-3, 4.6-1, 4.8-2
part number, 8.1-6, 8.2-4
removal procedures, 4.8-1
Software Fatal Error, 7.4-2, 7.4-5
support circuitry, 2.4-4
System Control, 2.4-1
table showing adjustments, A.1-1
table showing switch and jumper settings, A.2-4
tools/supplies needed for replacement, 4.8-1
Unable to Create INF File, 7.4-5
Utility Timer circuit, 2.4-4
verification procedures, 4.8-2
User Resource Adapter card. See URA card
Utility Timer circuit
description, 2.4-4
location, 2.4-5
V
VAC ON signal
AC Power/Vacuum Relay card, 2.2-2, 2.2-5
location, 2.2-3
VACUUM ADJUST
description, 7.1-2
vacuum adjustment procedure
steps, 4.30-1
Vacuum Sensor card, 4.14-2
Vacuum Out of Range
description, 7.4-5, C.1-1
vacuum pump
AC Power/Vacuum Relay card, 2.2-5
installation procedures, 4.23-1
location, 2.2-4, 4.4-1
part number, 8.1-4, 8.2-3
removal procedures, 4.23-1
tools/supplies needed for replacement, 4.23-1
unpacking the instrument, 3.2-1
12-INDEX
VACUUM ADJUST, 7.1-2
verification procedures, 4.23-1
vacuum regulator
adjustment, 2.3-9
description, 2.3-9
location on Fluidics Panel, 2.3-1
part number, 8.1-3, 8.2-2
Vacuum Sensor card
adjustments, 2.5-5
function, 2.5-4
input/output voltage, 2.5-4
installation procedures, 4.14-2
location, 2.2-3, 4.4-1
location of connectors, 2.5-6
part number, 8.1-3, 8.2-3
removal procedures, 4.14-1
Service Diagnostic, 7.3-1
solenoid valves, 2.5-4
table of test points, 2.5-6, A.2-4
test point locations, 2.5-6
tools/supplies needed for replacement, 4.14-1
vacuum adjustment procedure, 4.14-2
verification procedures, 4.14-2
VACUUM SENSOR signal
location, 2.4-5
VIC
description, 2.3-2
function, 2.5-4
part number, 8.1-4, 8.2-9
tube removal, 4.14-2
VOLTAGE READINGS
description, 7.1-2
W
warning
definition, 1.1-1
WBC APERTURE signal
table showing Sensor Preamp Adapter card
connections, 2.5-1
WBC bath
activity on power-up cycle, 2.1-6
description, 2.3-2
WBC PREAMP signal
table showing SPAD card connections, 2.5-9
WBC signal
table showing Sensor Preamp Adapter card
connections, 2.5-2
PN 4237242
C
TRADEMARKS
“CC” logo, COULTER, COULTER CLENZ and 4C are trademarks of Coulter International Corp.
Centronics is a registered trademark of Centronics Data Computer Corporation.
CITIZEN is a registered trademark of Citizen America Corporation.
DOW CORNING 33 is a registered trademark of Dow Corning Corporation.
Epson is a registered trademark of Epson America, Inc.
FLUKE is a registered trademark of John Fluke Mfg. Co., Inc.
IBM is a registered trademark of International Business Machines, Inc.
LOCTITE is a registered trademark of Loctite Corporation.
MATE-N-LOK is a registered trademark of Amp Inc.
PharMed is a registered trademark of Norton Co.
PN 4237242C
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