Biotecnica Instruments BT3000 PLUS analyzer system SERVICE MANUAL

BT3000 PLUS

Technology Advanced Random Generation Analyzer System

SERVICE MANUAL

P/N: MS-04444-05-ING

Software Version 8

Rev. 0, September 9, 2004

This product conforms to the safety requirements of the Council

Directives 98/79/EEC of 27 October 1998 (European Parliament) regarding the In-Vitro Diagnostic Medical Devices. This directive is in accordance with the Article 2, Paragraph 2 of the Directive 89/336/EEC, which ceases to apply to the products complying with the present directive. Refer to Paragraph 7, Article No.1 of the IEC Official Gazette

No. L331 of Dec. 1998.

It also conforms to Italian Regulations CEI EN 61010-01 and CEI EN

61326-1 (EMC) .

The conformity is attested when the equipment is installed in accordance with the conditions outlined in the manual

MANUAL REVISION RECORD

Rev. No.

This record page is for recording revisions to your BT3000 PLUS manual that has been published by Biotecnica Instruments S.p.A. We recommend that only the management or facility representative authorized to process changes and revisions to publications observe as follows:

Make the pen changes or insert the revised pages.

Ensure that obsolete pages are withdrawn and either disposed of immediately, or marked as superseded and placed in a superseded document file.

Enter the information below reflecting that the revisions have been entered.

Date Description Approved By

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BT 3000 PLUS - TABLE OF CONTENTS

SECTION A

• WARRANTY CONDITIONS

• INSTRUMENTS RETURN AUTHORIZATION MOD.05-35a

• POTENTIAL RISKS DURING OPERATION & MAINTENANCE

• WARNINGS

• CONFORMANCE

• THE COMPANY PROFILE

• PACKING AND UNPACKING THE ANALYZER

SECTION I

INTRODUCTION - BT3000 PLUS SYSTEM

1-1.

1-2.

1-3.

1-4.

1-5.

1-6.

1-7.

EXAMPLES OF THE BASIC OPERATIONS OF CLIN. CHEM..... I

PHASE 1: CREATION OF THE WORKLIST……………...........…. I

PHASE 2: PLACEMENT OF SAMPLES AND REAGENTS……... I

PHASE 3: START-UP.................................................................... I

PHASE 4: PROCESS INITIATION................................................ I

PHASE 5: READING CYCLE………………................................... I

EXAMPLE OF BASIC ELECTROLYTES OPERATION……......... I

2-1.

2-2.

2-3.

2-4.

2-4.1.

SECTION II

TECHNICAL SPECIFICATIONS

PHYSICAL DIMENSION OF BT 3000 PLUS......................…....... II

POWER SUPPLY REQUIREMENTS............................................. II

EMC AND ELECTRICAL SAFETY..............…………………......... II

PERFORMANCE & TECHNICAL SPECS..................................... II

OPERATING AMBIENT CONDITIONS......................................... II

2-4.4. "UTILITY" EXECUTION TIMES..................................................... II

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BT3000 PLUS - TABLE OF CONTENTS

2-8.

2-9.

2-10.

DILUTER LINEARITY AND ACCURACY........………………….... II

PHOTOMETER LINEARITY AND ACCURACY............................ II

PHOTOMETER TUNGSTEN HALOGEN LAMP……..….............. II

2-13. WASHING SOLUTION SUPPLY................................................... II

3-1.

3-3.

SECTION III

ELECTRONICS

TOP VIEW OF THE BT 3000 PLUS.............................................. III

DESCRIPTION OF ANALYZER MODULES................................ III

3-3.3. POWER SUPPLY MODULE.......................................................... III

3-3.7.

3-5.

DUAL SAMPLING ARMS MODULE ............................................. III

EXAMPLE OF THE I 2 C-BUS CONFIGURAZIONE....................... III

SECTION IV

SOFTWARE

4-1. GENERAL...................................................................................... IV

4-4.

4-4.1.

4-5.

4-6.

CALCULATION OF CHECK-SUM................................................. IV

ADDITIONAL NOTES ON THE SERIAL PROTOCOL.................. IV

WIRING DIAGRAM OF INTERFACE CABLE................................ IV

VARIABLE SERIAL PROTOCOL................................................... IV

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4-7.

BT3000 PLUS - TABLE OF CONTENTS

SERIAL COMMUNICATION TEST PROGRAMS.......................... IV

5-3.3.

5-3.4.

5-4.

5-4.1.

5-4.2.

5-4.3.

5-4.4.

5-4.5.

5-4.6.

5-1.

5-2.

5-2.1.

5-2.2.

5-3.

5-3.1.

5-3.2.

SECTION V

REPAIR OR REPLACEMENTOF VARIOUS PARTS

ACCESS TO VERIOUS MODULES……………………….............. V

REPAIR OR REPLACEMENT OF THE SAMPLING ARM............. V

ARM HEAD RETROFIT KIT INSTALLATION INSTRUCTIONS.... V

SAMPLING TUBE REPLACEMENT PROCEDURE...................... V

REPAIR OR REPLACEMENT OF THE REAGENTS MODULE.... V

REPLACEMENT OF REAGENT PELTIER MODULES................. V

REPLACEMENT OF REAG. PLATE TRANSMISSION BELT…... V

REPLACEMENT OF REAG. PLATE DRIVE MOTOR.................... V

REPLACEMENT OF REAG. TEMP/HALL SENSORS................... V

REPAIR OR REPLACEMENT OF THE READING STATION….... V

REPLACEMENT OF CUVETTES PELTIER.................................. V

REPLACEMENT OF THE HALOGEN LAMP................................. V

REPLACEMENT OF HYDRAULIC TUBES.................................... V

REPLACEMENT OR MAINTENANCE OF DILUTER.................... V

REPLACEMENT OF THE WASHING PISTON.............................. V

REPLACEMENT OF READING CUVETTE................................... V

5-5.

5-5.1.

INTRODUCTION TO THE COMPUTER BOX…........................... V

REPAIR AND REPLACEMENT OF COMPUTER BOX................. V

5-5.2. 8-WIRE ANALOGUE RESISTIVE TOUCH-SCREEN................... V

Fig.5-1 BT3000 PLUS - EXPLODED VIEW………………………………… V

Fig.5-3 SAMPLING ARM (REAR).............................................................. V

Fig.5-4 ARM HEAD PLACEMENT............................................................. V

Fig.5-5 METALLIC FIN INSERTION INTO THE ARM HEAD.................... V

Fig.5-6 ARM HEAD COVER REPLACEMENT/REMOVAL........................ V

Fig.5-7 SAMPLING TUBE WITH NEEDLE HOLDER................................ V

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BT3000 PLUS - TABLE OF CONTENTS

Fig.5-8 ARM HEAD (TOP VIEW)............................................................... V

Fig.5-9 ARM HEAD (BOTTOM VIEW)....................................................... V

Fig.5-10 ARM HEAD (SIDE VIEW).............................................................. V

Fig.5-11 SAMPLING TUBE WITH NEEDLE HOLDER................................ V

Fig.5-12 SAMPLING TUBE WITH NEEDLE HOLDER................................ V

Fig.5-13 ARM HEAD COVER REMOVAL.................................................... V

Fig.5-14 SAMPLING TUBE DISCONNECTION........................................... V

Fig.5-15 NEEDLE HOLDER CLAMP LOOSENING..................................... V

Fig.5-16 SAMPLING TUBE REMOVAL....................................................... V

Fig.5-17 SAMPLING TUBE REMOVAL....................................................... V

Fig.5-18 REPLACING THE HALOGEN LAMP............................................. V

Fig.5-19 LAMP REMOVAL (REAR COVER)................................................ V

Fig.5-20 LAMP REMOVAL (FRONT SHUTTER))........................................ V

Fig.5-21 HYDRAULIC TUBES AND ACCESSORIES.................................. V

Fig.5-23 READING STATION - BOTTOM VIEW (REF. CHANNEL)............ V

Fig.5-24 FUNNEL CAP OPENER................................................................ V

Fig.5-25 WASHING PISTON REMOVAL & CLEANING.............................. V

SECTION VI

READING STATION SYSTEM

6-2.1.

6-3.

6-4.

6-4.1.

6-5.

6-6.

6-7.

6-8.

6-9.

REACTION CUVETTES OPERATING PRINCIPLE..........……... VI

READING CUVETTES SYSTEM.................................................. VI

DILUTER AND WASHER MODULE............................................. VI

DILUTER OPERATING PRINCIPLE.....................….................... VI

ELECTRONICS FOR THE READING STATION SYSTEM........... VI

MAIN LOGIC BOARD (15-04211-01)............................................ VI

MAIN POWER BOARD (15-04209-01).......................................... VI

INTERFACE BOARD (15-04270-01)............................................. VI

THE 10 - CHANNEL PHOTOMETER............................................ VI

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6-9.1.

BT3000 PLUS - TABLE OF CONTENTS

TECHNICAL SPECIFICATIONS OF THE PHOTOMETER........... VI

Fig.6-2 READING STATION - BOTTOM VIEW.......................................... VI

Fig.6-4 DILUTER/WASHER MODULE AND WATER RESERVOIR....….. VI

Fig.6-6 READING STATION - REAR VIEW............................................... VI

SECTION VII

REAGENTS MODULE

7-1. GENERAL................................................................................…... VII

7-2. REAGENT PLATE CONTROLLER 15-05211-01........................... VII

7-3.

7-4.

REAGENT PLATE CONTROLLER 15-04313-03 - OBSOLETE... VII

REAGENT REFRIGERATION SYSTEM........................…..…...... VII

7-5. REAGENT & SERUM BARCODES............................................... VII

Fig.7-1 REAGENT REFRIGERATOR MODULE...........................…......... VII

Fig.7-2 REAGENT REFRIGERATION SYSTEM - EXPLODED VIEW...... VII

Fig.7-4 REAGENT CONTROLLER MODULE....................................….... VII

SECTION VIII

SAMPLING STATION

8-2.

8-2.1.

8-2.2.

8-2.3.

8-3.

SAMPLING ARM MODULE........................................................... VIII

ARM CONTROLLER BOARD (15-04765-01)............................... VIII

ARM LIQUID SENSOR & EMITTER BOARD (15-05023-01)........ VIII

ISE SAMPLING ARM MODULE.................................................... VIII

THE SERUM MODULE................................................................. VIII

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BT3000 PLUS - TABLE OF CONTENTS

8-3.1.

8-3.2.

8-3.3.

8-3.4.

SERUM PLATE CONTROLLERS.................................................. VIII

SERUM PLATE CONTROLLER BOARD (15-04422-01).............. VIII

LEFT SERUM PRIMARY TUBE SENSOR BOARD (15-04280-01)...

VIII

RIGHT SERUM PRIMARY TUBE SENSOR BOARD

(15-04278-01)...

VIII

8-5. SERUM & REAGENT BARCODES............................................... VIII

Fig.8-1 SAMPLING ARM WITH HEATER................................................. VIII

Fig.8-2 SAMPLING ARM MODULE........................................................... VIII

Fig.8-4 SAMPLING ARM (REAR)............................................................ VIII

MODULE.........................................................................

Fig.8-6 SERUM PLATE CONTROLLER MODULE................................... VIII

Fig.8-7 SERUM MODULE - EXPLODED VIEW........................................ VIII

SECTION IX

ISE MODULE

9-2.

9-2.1.

9-2.2.

SUBASSEMBLIES OF THE ISE MODULE.................................... IX

ISE PREAMPLIFIER BOARD (15-04440-01)................................ IX

ISE CONTROLLER BOARD (15-04320-01)................................. IX

9-3.

9-4.

COMMONLY ASKED ISE QUESTIONS…………………………… IX

ISE TROUBLE-SHOOTING BT3000 PLUS................................... IX

Fig.9-1. ISE MODULE ARRANGEMENT - FRONT VIEW.......................... IX

Fig.9-2. ISE MODULE - VIEW "A"............................................................... IX

Fig.9-3. ISE MODULE - VIEW "B"............................................................... IX

Fig.9-4. ISE MODULE - ELECTRODES LOCATION.................................. IX

SECTION X

COMPUTER MODULE

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BT3000 PLUS - TABLE OF CONTENTS

10-1.3. LCD MODULE 8-WIRE ANAL.RESIS.TOUCH-SCREEN.............. X

10-1.4. POWER SUPPLY MODULE.......................................................... X

10-1.5. MOTHERBOARD LAYOUT CONTENTS...................................... X

SECTION XI

POWER SUPPLY

11-1.1. EMC DIRECTIVE APPLICATION TO THE PWR SUP.................. XI

11-1.2. POWER FACTOR CORRECTION (PFC)………...………............. XI

11-1.3. INHIBIT OPTION (INH)........................................…………............ XI

SECTION XII

VACUUM PUMP SYSTEM P/N 06-05161-01

12-1.2. DESCRIPTION OF THE BOARD 15-05085-01............................. XII

12-1.3. VACUUM PUMP SYSTEM COMPONENTS.................................. XII

12-1.5. MAINTENANCE AND CARE.......................................................... XII

12-1.6. TROUBLE-SHOOTING.................................................................. XII

12-1.7. SPARE PARTS FOR REPAIR AND MAINTENANCE................... XII

Fig.12-1 VACUUM PUMP SYSTEM P/N 06-05161-01................................ XII

VACUUM PUMP SYSTEM, P/N 662.0788 & BY-PRODUCTS

PREVIOUS VERSION (OBSOLETE)

GAUGE.........................................................................

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BT3000 PLUS - TABLE OF CONTENTS

12-2.2. VACUUM LEVEL REGULATOR.................................................... XII

FILTER...............................................................

12-2.4. CAUTION! SHIPPING RETAINER - VACUUM PUMP SYSTEM.. XII

12-2.5. INSTALLATION.............................................................................

12-2.6. QUICK

12-2.7. CARE AND MAINTENANCE......................................................... XII

12-2.8. FUSE REPLACEMENT FOR MAIN POWER INLET MODULE..... XII

REPLACEMENT...............................................................

12-2.10. PERISATLTIC PUMP CARTRIDGE REPLACEMENT.................. XII

12-2.11. VACUUM REGULATION PROCEDURE....................................... XII

12-2.12. PRINCIPLES OF OPERATION..................................................... XII

12-2.13. TECHNICAL DESCRIPTION OF BOARD (15-04541-01)............. XII

GUIDE.....................................................

12-2.15. SPARE PARTS FOR REPAIR AND MAINTENANCE................... XII

SPECIFICATIONS...................................................

12-2.17. CLEANING OF THE HYDROPHOBIC FILTER............................. XII

Fig.12-4 VACUUM PUMP SYSTEM INSTALLATION................................. XII

Fig.12-5 SPARE PARTS FOR MAINTENANCE AND REPAIR................... XII

Fig.12-6 WASTE CHAMBER LEFT AND RIGHT......................................... XII

Fig.12-7 DETAILS OF SOME SPARE PARTS............................................ XII

Fig.12-8 WASTE PROBE............................................................................. XII

Fig.12-9

SPARE CABLES FOR VACUUM PUMP SYSTEM....................... XII

Fig.12-10 VACUUM PUMP SYSTEM WARNINGS....................................... XII

SECTION XIII

MEASUREMENTS AND CALIBRATIONS

13-1.1. PHOTOMETER CALIBRATION AND SPECIFICATIONS.............. XIII

13-1.2. PHOTOMETER CALIBRATION PROCEDURE............................. XIII

13-1.3. VERIFICATION OF ANY PROBLEMS DURING THE READING.. XIII

13-2. CUVETTES CALIBRATION AND TEMP. CONTROL SPECS....... XIII

13-2.1. CUVETTE TEMPERATURE CALIBRATION PROCEDURES . XIII

13-3. REFRIGERATION SYSTEM CALIBRATION AND SPECS........... XIII

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BT3000 PLUS - TABLE OF CONTENTS

13-3.1. REAGENT CONTROLLER P/N 15-04313-03 (OBSOLETE)......... XIII

13-3.2. REAGENT CONTROLLER BOARD P/N 15-05211-01.................. XIII

13-4. BARCODE CALIBRATION AND SPECIFICATIONS.................... XIII

13-4.1. PROGRAMMING OF SERUM/REAGENT BARCODE.................. XIII

13-4.2. BARCODE - MECHANICAL CALIBRATION PROCEDURE......... XIII

13-5. ISE MODULE CALIBRATION AND SPECIFICATIONS................ XIII

13-5.1. ISE BUBBLE SENSOR CALIBRATION PROCEDURE................. XIII

13-5.2. ISE HYDRAULIC CIRCUIT VERIFICATION.................................. XIII

13-5.3. ISE ELECTRODES POTENTIAL VERIFICATION......................... XIII

13-6. PRIMARY TUBE SENSOR CALIBRATION AND SPECS............. XIII

SECTION XIV

TROUBLE-SHOOTING

TEMPERATURE...........................…………..............

14-1.2. REAGENT CHAMBER TEMPERATURE..........………............... XIV

14-1.3. POWER SUPPLY PROBLEMS...........................………............... XIV

14-1.4. POWER UP PROBLEMS.....................................……….............. XIV

14-1.5. THE MOUSE AND KEYBOARD..........................………............... XIV

14-1.6.

RESET ERRORS: SERUM PLATE / REAGENT TRAY

DILUTER / SAMPLING ARM.................................……….............

XIV

14-2.

14-2.1

INTRODUCTION - COMPUTER MODULE TROUBLE-

SHOOTING....................................................................................

XIV

COMPUTER MODULE TROUBLE-SHOOTING.....…………........ XIV

14-2.2

14-3.

14-4.

BOOTSTRAP - COMPUTER MODULE TROUBLESHOOTING.... XIV

UNRELIABLE RESULTS OF ANALYSES - GENERAL

CONSIDERATIONS.......................................................................

XIV

VERIFICATION OF PHOTOMETRIC INSTABILITY XIV

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BT3000 PLUS - TABLE OF CONTENTS

14-5.

14-6.

VERIFICATION OF HYDRAULIC SYSTEM OPERATION............ XIV

ISE TROUBLESHOOTING............................................................ b) c) d) e)

OBSTRUCTIONS CAUSED BY PROTEINS IN ELECTRODES CL & CO

2

BAD RESULTS OF SAMPLES

ERROR MESSAGES, AIR BUBBLES, FLAGS

RESULTS WITH FLAG "D"

XIV

Fig. 14-1 CHLORIDE ELECTRODE CLEANING PROCEDURE XIV

Fig. 14-2 ISE ELECTRODES CLEANING PROCEDURE

SECTION XV

SPARE PARTS FOR REPAIR AND MAINTENANCE

XIV

15-1.

15-2.

15-3.

15-4.

15-5.

READING STATION MODULE SPARE PARTS........................... XV

REAGENT REFRIGERATION SYSTEM SPARE PARTS............. XV

SERUM MODULE SPARE PARTS............................................... XV

SAMPLER ARM SPARE PARTS.................................................. XV

ISE MODULE SPARE PARTS...................................................... XV

15-9. VACUUM PUMP SYSTEM SPARE PARTS................................. XV

INDEX - BT3000 PLUS REV. 0, Soft Ver. 8 Page 13 of 15

SCHEMATIC

BT3000 PLUS - TABLE OF CONTENTS

SCHEMATICS AND DIAGRAMS

ELECTRONICS SYSTEM

TITLE

SE-04270-01

SE-04278-01

SE-04280-01

SE-04282-01

SE-04408-01

SE-04408-02

SE-04422-01

READING PLATE INTERFACE

SERUM PRIMARY TUBE SENSOR "R"

SERUM PRIMARY TUBE SENSOR "L"

SERUM HALL SENSOR

MOTOR DRIVER BOARD

MOTOR DRIVER BOARD ISE

SERUM PLATE CONTROLLER

SE-04661-01

SE-04765-01

SE-04765-02

SE-04767-01

SE-05021-01

SE-05023-01

SE-05211-01

DIAGRAM

SE-04334-03

SE-04348-02

SE-04379-02

SE-04436-03

SE-04437-03

SE-04438-02

SE-04443-02

POWER SUPPLY INTERFACE

LEFT ARM CONTROLLER

RIGHT ARM CONTROLLER

ANGULAR POSITION ENCODER

DILUTER ENDSTROKE SENSOR

ARM LIQUID SENSOR & EMITTER

REAGENT PLATE CONTROLLER

BLOCK DIAGRAMS

TITLE

READING PLATE ASSY

SERUM PLATE ASSY

BOX COMPUTER ASSY

LEFT ARM ASSY

RIGHT ARM ASSY

ARM WITH HEATING TUBE

REAGENTS PLATE ASSY

<< Vacuum Pump System on the next page>>

INDEX - BT3000 PLUS REV. 0, Soft Ver. 8 Page 14 of 15

BT 3000 PLUS - TABLE OF CONTENTS

VACUUM PUMP SYSTEM P/N 06-05161-01/02/03

SCHEMATIC

SE-05085-01

SE-05161-01

SE-05165-01

TITLE

VACUUM PUMP SYSTEM CONTROLLER BOARD

VACUUM PUMP SYSTEM BLOCK DIAGRAM

WASTE PROBE CONNECTION DIAGRAM

VACUUM PUMP SYSTEM P/N 662.0788/C/D/E/F - OBSOLETE

SE-04541-01 CONTROLLER #VACUUM PUMP SYSTEM

SE-662.0788

SE-662.0788A

SE-662.0788B

SE-662.0788E

VACUUM PUMP SYSTEM (220 VAC)

WASTE PROBE "L"

WASTE PROBE "R"

VACUUM PUMP SYSTEM (110 VAC)

INDEX - BT3000 PLUS REV. 0, Soft Ver. 8 Page 15 of 15

WARRANTY CONDITIONS

SECTION A

• Biotecnica Instruments S.p.A., after having accurately tested this analyzer, guarantees the instrument for 1 (one) year starting from invoice.

• The warranty includes the repairing and the replacement for free of the faulty parts due to wrong manufacturing. Warranty is not extended to the normally consumable parts of the system.

• The warranty is not valid in case of improper use, negligence, improper or lack of maintenance and cleaning, tampering or repairing by third parties not authorized by Biotecnica Instruments

S.p.A. and in any case when the cause cannot be stated as original manufacturing fault.

• The costs of shipment and transport to Biotecnica Instruments S.p.A. for repair or substitution, and the risks deriving from this is the responsibility of the buyer, including all the costs of onsite technical service at client's location (transport, board and lodging) as well.

• If the stated defects will result to be out of warranty limits, the buyer will pay repair or replacement costs.

• Biotecnica Instruments S.p.A. is not responsible for any unforeseen technical problem that might occur. If the requested technical assistance is outside the terms of warranty a charge will me made to the customer as per current rates in force.

• Biotecnica Instruments S.p.A. is an internationally known for its high quality standards in production. Biotecnica Instruments S.p.A. is thus responsible for providing to the customer clear and effective information for use of it's products, including all the precautions and warnings for a secure and risk-free use.

• Service personnel must also refer to the warnings and cautions notices in this manual. It is the duty of the service engineer of Biotecnica to instruct his service personnel to take all necessary precautions during repair and handling of products.

• Biotecnica Instruments S.p.A. is not responsible for any damage that may be caused directly or indirectly to persons or things due to a lack of observance of all the warnings and cautions outlined in the user's manual, and concerning the warnings and cautions during the different working phases of the instrument (see chap. M). Direct, indirect, incidental, special, moral damages as well as other damages of any type (including, with no limitation, those deriving from profit’s loss, business interruption or information loss) cannot be ascribed to Biotecnica

Instruments S.p.A. even in the case in which the possibility of the event had been explicitly stated.

Biotecnica Instruments S.p.A.

Via Licenza, 18

00156 Rome (ITALY)

Tel. +39-06-4112316

Fax +39-06-4103079

E-mail: [email protected]

Web: www.biotecnica.it

© 2004 BIOTECNICA INSTRUMENTS S.p.A., ROME, ITALY

INFORMATION CONTAINED IN THIS MANUAL IS PROPRIETARY WITH

BIOTECNICA INSTRUMENTS S.p.A.. REPRODUCTION OF ANY PART OR

WHOLE MAY ONLY BE PERFORMED WITH WRITTEN PERMISSION

FROM BIOTECNICA INSTRUMENTS S.p.A.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 1 of 12

Page 1 of 1

Mod.05-35a-ing Rev. 0

Parts / Instruments Return Authorization

From:

To: Technical Assistance – Export Manager

DATE: ______/______/____

Client information

Instrument model:

Defective part

Serial number:

Part description:

Code:

Bar-code number:

Serial number:

Quantity:

Under warranty: Yes No Order number:

Description of the problem:

Date:

Request for:

Repair Exchange Quotation Urgent

Name: Signature:

Biotecnica Instruments response

Return authorization number:

/ 2004

Approved: Yes No

Date:

Repair Exchange Destroy Quotation:

Note:

Approved by: Technical Assistance Dept. Signature:

Quality Control Manager Supervising Signature:

Note: No parts or instruments will be accepted for repair or replacement without a Return Authorization number, which can be obtained from Biotecnica Instruments S.p.A. Fax this Return Authorization form to

+39 06 410 3079 to the attention of Technical Assistance/Export Manager, who will then evaluate and issue a

Return Authorization number.

WARRANTY EXCTRACT: Biotecnica Instruments S.p.A. warrants its instruments to be free from defective parts and workmanship for a period of one (1) year from the date of purchase. Liability under this warranty is expressly limited to repair or replacement of defective parts at the option of Biotecnica Instruments S.p.A. This warranty does not cover the results of misuse, accident or abuse of any parts of its instruments which have been repaired, tampered with or altered by anyone other than personnel authorized by Biotecnica Instruments S.p.A. This warranty does not apply to fluid handling devices, consumables or reagents.

Products returned to Biotecnica Instruments S.p.A. for repair or replacement shall be sent with transportation prepaid.

If found not to be defective under the terms of warranty, a charge will be made for repair or replacement and freight costs will be at customer’s expense.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 2 of 12

POTENTIAL RISKS DURING OPERATION AND MAINTENANCE

USE

Although the BT3000 PLUS analyzer system uses high performance components, which provide a high degree of safety, it is essential that the user takes the usual precautions to safeguard himself and to ensure a safe working environment.

Biotecnica Instruments S.p.A. only guarantees the workmanship and materials of its products. It is the duty of the user to take care of safe operation and no amount of warnings can take place of such care.

As regards the moving parts in the analyzer, these have been appropriately protected to avoid any potential risks to the user, and for proper instrument operation and safety. However, it is highly recommended to exercise extreme care during analyzer operation and especially when working close to the devices.

To avoid accidental contamination, use suitable guards and/or personal protection, such as overall and gloves. When handling reagents, it is advisable to observe good laboratory practice (GLP) rules.

Chemicals, serum samples and reagents must be handled with extreme caution. Patient samples may be biologically hazardous. The reagents or any other substances that may enter in contact with samples should be treated in the same way as samples themselves.

The materials of human origin, such as control sera, are tested for the detection of HbsAg, anti-HCV anti-HIV-1 anti-HIV–2 antibodies. Even if the result is negative, as no known analytical method can exclude any infection’s risk with certainty therefore these materials must be considered as potentially infective and thus must be handled with

extreme caution. The reagents and any other substance entering in contact with samples must be treated in the same way. The reagents must be manipulated (before, during and after the use) by qualified personnel familiar with their characteristics in order to safeguard the user as well as the quality of the reagent itself.

MAINTENANCE

• It is of extreme importance that the instrument is fully turned off and the power cord unplugged from the wall outlet to safely perform any maintenance or service procedure.

• During maintenance procedures (see "Chapter N, “Maintenance“), the safety and warning precautions must be observed as outlined in the preceding paragraph.

• The exterior of the analyzer casing may be cleaned periodically to remove dust grease and other contamination. It is not necessary to clean the inside.

Use soft cloth dampened with a mild solution of detergent with water.

• The owner shall be responsible for maintenance of the analyzer. Wear or damage caused by lack of normal maintenance or by misuse of the analyzer shall not be considered as defective workmanship and material.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 3 of 12

WARNINGS

ALTHOUGH THE BT3000 PLUS ANALYZER SYSTEM USES HIGH PERFORMANCE

COMPONENTS WHICH PROVIDE A HIGH DEGREE OF SAFETY, IT IS ESSENTIAL THAT THE

SERVICE ENGINEER TAKES THE USUAL PRECAUTIONS TO SAFEGUARD HIS SERVICE

PERSONNEL. WE ONLY GUARANTEE THE WORKMANSHIP AND MATERIALS OF OUR

PRODUCTS. IT IS THE DUTY OF THE SERVICE ENGINEER TO TAKE CARE FOR SAFE

OPERATION AND NO AMOUNT OF WARNINGS CAN TAKE THIS PLACE OF SUCH CARE.

THE FOLLOWING WARNINGS WILL AID THE SERVICE PERSONNEL TO PROVIDE

ADEQUATE SAFEGUARDS TO ASSURE SAFE TROUBLE-FREE PERFORMANCE:

1. BEFORE SERVICING THIS SYSTEM, BE SURE TO READ THE SERVICE MANUAL

THOURALLY AND CAREFULLY. AFTERWARDS, KEEP IT HANDY FOR FUTURE

REFERENCE.

2. TAKE SPECIAL CARE TO FOLLOW THE WARNINGS AND CAUTIONS INDICATED ON

THE SYSTEM REAR PANEL AS WELL AS IN THE SERVICE MANUAL.

3. SERVICING OF THIS SYSTEM SHOULD BE RESTRICTED TO QUALIFIED/APPROVED

SERVICE PERSONNEL.

4. SLOTS AND OPENINGS IN THE CASE, BACK PANEL, AND BOTTOM ARE PROVIDED

FOR VENTILATION. THIS ENSURE RELIABLE OPERATION OF THE SYSTEM AND TO

PROTECT IT FROM OVERHEATING. DO NOT BLOCK OR COVER THESE OPENINGS.

5. BEFORE SERVICING, CHECK THAT THE VOLTAGE ON THE REAR PANEL LABEL

MATCHES THE LOCAL LINE VOLTAGE.

6. TO GUARANTEE SAFETY THE SYSTEM MUST BE GROUNDED. THE WIRES IN THE

MAINS POWER CORDSET ARE COLORED IN ACCORDANCE WITH THE FOLLOWING

CODES:

GREEN AND YELLOW: EARTH

NEUTRAL

LIVE

THE WIRE COLORED BLUE IS INTERCHANGEABLE WITH WIRE COLORED BROWN.

7. REPLACE FUSE AS MARKED. PRIOR TO THE REMOVAL OF ANY FUSE, TURN POWER

OFF AND UNPLUG THE CORDSET FROM THE WALL.

8. TO REDUCE THE RISK OF ELECTRIC SHOCK. DO NOT REMOVE ANY PANEL OR

COVER UNDER POWER "ON" CONDITION. BEFORE REMOVING ANY PANEL OR

COVER, TURN POWER "OFF" AND UNPLUG THE POWER CORDSET FROM THE WALL

9. FOR OPERATING SAFETY, DO NOT INSTALL THE SYSTEM IN A LOCATION WHERE IT

WILL BE EXPOSED TO HEATING EQUIPMENT OR RADIATORS, DIRECT SUN LIGHT,

OR ANY OTHER SOURCE OF EXTREMELY HIGH TEMPERATURES.

10. DO NOT OPERATE THE SYSTEM IN THE PRESENCE OF FLAMMABLE FLUIDS OR

GASEOUS ATMOSPHERE, DISINFECTING AGENTS, CLEANING AGENTS, ETC., DUE

TO POSSIBLE FIRE OR EXPLOSION.

11. DO NOT KINK, BEND, LAY OBJECT ON, OR OTHERWISE DAMAGE OR RESTRICT

CABLES.

12. BE SURE THAT THE POWER SWITCH ON THE BACK PANEL OF SYSTEM IS OFF

WHEN PLUGGING IN, OR REMOVING THE POWER CORDSET FROM A WALL OUTLET.

13. TURN OFF THE MAINS POWER SWITCH WHENEVER THE SYSTEM IS NOT IN USE;

THE SYSTEM IS SWITCHED OFF BUT NOT ENTIRELY. TO TURN OFF POWER TO

WHOLE SYSTEM, UNPLUG POWER CORD FROM WALL OUTLET.

14. DO NOT ATTEMPT TO ALTER THE SHAPE OF ANY PART OF THE SYSTEM.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 4 of 12

15. IF THE SYSTEM IS NOT OPERATING PROPERLY AND THE TROUBLE-SHOOTING

SECTION DOES NOT PROVIDE A SATISFACTORY SOLUTION TO THE PROBLEM,

THEN DO NOT USE THE SYSTEM UNTIL THE DEFECTS ARE REMEDIED.

16. INSPECT ALL ACCESSORIES AND SYSTEM CORDS. DO NOT USE IF DAMAGE CAN

BE SEEN SUCH AS CUT INSULATION OR OUTER COVERING, FRAYED OR BROKEN

WIRES, CORRODED OR BROKEN CONNECTORS ETC.

17. TO REDUCE THE RISK OF FIRE OR ELECTRIC SHOCK, DO NOT ALLOW FLUIDS OR

ANY FOREIGN OBJECT TO ENTER THE SYSTEM. WIPE OFF SPILLS IMMEDIATELY.

18. DO NOT USE BENZENE, THINNER, ANY KIND OF SOLVENTS, OR ABRASIVE

DETERGENTS TO CLEAN THE CASE. CLEAN WITH SOFT DUSTING CLOTH

DAMPENED WITH DISTILLED WATER. IF NECESSARY USE ONLY NEUTRAL

DETERGENT.

19. DO NOT STICK OBJECTS OF ANY KIND INTO THE SYSTEM THROUGH BACK PANEL

OR CASE SLOTS AS THEY MAY TOUCH DANGEROUS VOLTAGE POINTS OR SHORT

OUT PARTS THAT COULD RESULT IN FIRE OR ELECTRIC SHOCK.

20. INSTALL THE SYSTEM IN SUCH A WAY THAT ADEQUATE VENTILATION IS PROVIDED

ALL AROUND TO PROPERLY DISSIPATE THE HEAT.

21. MAKE SURE ALL FLUID LINES ARE FREE OF KINKS, NICKS, SHARP BENDS,

PUNCTURES, OR OCCLUSIONS BEFORE INSTALLING ON SYSTEM.

22. DO NOT TWIST THE PERISTALTIC PUMP TUBING WHEN PLACING IN THE RACEWAY

OF THE PUMP ROLLER.

23. RELEASE THE PERISTALTIC PUMP TUBING WHENEVER THE SYSTEM IS UNUSED

FOR LONG PERIODS OF TIME.

24. IF IT IS NOT BE USED FOR SOME TIME, THE PINCH-VALVES TUBING SHOULD BE

RELEASED.

CAUTION

THE HYDRAULIC CIRCUIT SHOULD BE FULLY DISCHARGED AND THE WATER RESERVOIR

DISCONNECTED FROM THE CIRCUIT PRIOR TO RELEASING THE TUBES FROM THE PINCH VALVES. IF

THESE STEPS ARE NOT TAKEN, SERIOUS DAMAGE MAY BE CAUSED TO THE ANALYZER.

25. THE HALOGEN LAMP MUST BE REPLACED SOME MINUTES AFTER THE

INSTRUMENT HAS BEEN TURNED OFF AND POWER CORD UNPLUGGED.

26. ALWAYS ALLOW THE BURNT OUT LAMP TO COOL DOWN BEFORE HANDLING OR

ATTEMPTING REPLACEMENT.

27. NEVER TOUCH THE LAMP OR THE REFLECTOR WITH BARE FINGERS. USE A RAG

WHEN CHANGING.

28. IF THE LAMP IS TOUCHED INADVERTENTLY DURING INSTALLATION, CLEAN THE

LAMP OR REFLECTOR WITH ALCOHOL AND DRY WITH A CLEAN, SOFT CLOTH

BEFORE BURNING. CONTAMINATION OF THE LAMP OR REFLECTOR MAY REDUCE

LAMP PERFORMANCE.

29. THIS LAMP EMITS UV (ULTRAVIOLET) RADIATION. PROLONGED EXPOSURE TO THIS

LAMP MAY CAUSE SKIN AND EYE IRRITATION

30. USE ONLY ORIGINAL TUBING REPLACEMENTS-"MONTHLY AND QUARTERLY

REPLACEMENT KITS". DO NOT USE CONVENTIONAL TUBING. THIS WILL CAUSE

MALFUNCTION OF THE SYSTEM.

31. THE ANALYZER SYSTEM MUST NOT BE DISMANTLED OR REPAIRED BY ANYONE

WHO HAS NOT BEEN QUALIFIED BY THE MANUFACTURER. INCORRECT WORK MAY

CAUSE FIRE OR IRREPARABLE DAMAGE TO THE SYSTEM.

32. DO NOT OVERLOAD ACCESSORIES POWER OUTLETS AND EXTENSION CORDS AS

THIS CAN RESULT IN FIRE OR ELECTRIC SHOCK.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 5 of 12

33. DO NOT PLACE THE SYSTEM ON AN UNSTABLE CART, STAND, OR TABLE; THE

SYSTEM MAY FALL, CAUSING SERIOUS INJURY TO USER, AND SERIOUS DAMAGE

TO THE APPLIANCE. PLACE THE SYSTEM ON A STABLE, VIBRATION-FREE, LEVEL

TABLE OR CART.

34. USE ONLY SECURE POWER SOURCE TO PROTECT THE ANALYZER SYSTEM

AGAINST POWER SURGES. DISCONNECT TEMPORARILY THE ANALYZER POWER

CORD FROM THE WALL OUTLET IN CASE OF BAD ATMOSPHERIC CONDITIONS.

35. DO NOT USE VACUUM PUMP UNTIL THE TWO TRANSIT SCREWS (COLORED RED)

AT THE BOTTOM OF CABINET HAVE BEEN REMOVED (VALID FOR OLD VACUUM

PUMP SYSTEM P/N 662.0788 AND BY-PRODUCTS).

36. DO NOT OIL ANY PART OF THE SYSTEM.

37. EMPTY WASTE CONTAINERS WHENEVER THEY ARE FULL. ENSURE THAT THE

CONTAINER LIDS ARE SCREWED ON TIGHTLY TO PREVENT LEAKAGE OR

DISPERSION INTO THE ENVIRONMENT.

38. THE SAFE DISPOSAL OF THE ANALYZER WASTE MATERIAL WITH MINIMAL

ENVIRONMENTAL IMPACT IS THE RESPONSIBILITY OF THE USER.

39. DO NOT ATTEMPT TO REMOVE ANY PANELS OR COVERINGS OF THE ANALYZER OR

THE VACUUM PUMP SYSTEM WHILE THE SYSTEM IS IN OPERATION.

40. DO NOT USE SOFTWARE DISKS OF UNKNOWN ORIGIN IN THE ANALYZER

COMPUTER AS THEY MAY INTRODUCE VIRUSES.

41. DO NOT USE THE COMPUTER OF THE ANALYZER FOR ANY OTHER PURPOSE THAN

THE ONE FOR WHICH IT IS DESIGNED FOR.

42. AFTER OPERATION/SERVICING, COVER THE SYSTEM WITH A PROTECTIVE PLASTIC

OR CLOTH SHEET.

43. BE PARTICULARLY CAUTIOUS THAT NO PARTS OF YOUR BODY (e.g. FINGERS HAIR,

ETC.) OR LOOSE OBJECTS (e.g. CABLES, TUBING, ETC.) CAN BE TRAPPED BY ANY

MOVING OR ROTATING PARTS (e.g. SAMPLING ARM, PLATES, WASHER MODULE,

NOTE: a)

PUMP ROLLERS ETC.) OF THE ANALYZER SYSTEM. b)

THE CAREFUL OBSERVATION OF THE PROCEEDING WARNINGS SHOULD RESULT IN A LONG AND

SATISFACTORY PERFORMANCE.

THIS INFORMATION IS BELIEVED TO BE CURRENT AT THE TIME OF PUBLICATION, BUT IT IS

PROVIDED WITHOUT WARRANTY OF ANY KIND AND WE ASSUME NO RESPONSIBILITY WITH

RESPECT THERETO.

WASTE DISPOSAL

• To ensure environment health and safety, it is recommended not to discard the used consumables, waste liquids or disposable maintenance kits into the environment.

• Insure that the disposal of waste material is done according to all applicable laws and regulations.

SAFE DISPOSAL OF THE UNUSABLE INSTRUMENT

For the proper disposal of instrument that is obsolete, outdated, beyond repair, or unusable, follow these steps carefully:

1) Remove all consumables from the instrument (test tubes, cups, bottles, containers, fluidic tubing etc.), and thoroughly clean the instrument with an approved disinfectant for decontamination.

2) Contact a firm specialized in "hazardous waste disposal" for safe instrument disposal.

NOTE: a. The manufacturer of the instrument assumes no responsibility for any breaches resulting from the delivery of the analyzer to a third party for disposal. b. The manufacturer of the instrument is not responsible for collection and safe disposal of the instrument. The safe disposal of the instrument and its contents is the responsibility of the user.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 6 of 12

a)

DANGER b) c) d)

TO AVOID ACCIDENTAL CONTAMINATION WITH CHEMICALS, PATHOLAGICALS,

AND MICROBIAL CONTAMINANTS, USE SUITABLE GUARDS AND/OR PERSONAL

PROTECTION-WHEN HANDLING CHEMICALS, SERUMS, REAGENTS, ETC.

THE SAFE DISPOSAL OF THE WASTE MATERIAL IS THE RESPONSIBILITY OF THE

USER.

INSURE THAT THE DISPOSAL OF RINSE WATER IS DONE ACCORDING TO ALL

APPLICABLE LAWS AND REGULATIONS.

ACCIDENTAL INJECTION OR PRICKING OF THE SKIN WITH ANY SHARP OBJECT

(NEEDLES, ETC.) MAY CAUSE A PROLONGED AND VERY PAINFUL LOCAL

ANTIMICROBACTERIAL INFLAMMATORY REACTION. EVERY PRECAUTION SHOULD

BE TAKEN TO AVOID SUCH ACCIDENTS.

I M P O R T AN T N O T I C E

THE "WARNINGS" INFORMATION ITSELF IMPLIES MANY NATURAL AND ACCEPTED

PRECAUTIONS. THE "WARNINGS" INFORMATION, THEREFORE, IS NORMALLY USED

TO CALL ATTENTION ONLY TO THE MOST IMPORTANT PRECAUTIONS, OR TO THOSE

THAT ARE NOT NECESSARILY LISTED WITH THE INSTRUMENT COMPONENTS.

THE RESPONSIBILITY FOR THE SAFE USE OF THE INSTRUMENT OF "BIOTECNICA

INSTRUMENTS S.p.A." RESTS WITH THE CUSTOMER. THE INSTRUMENT SHOULD BE

OPERATED OR SERVICED BY THE QUALIFIED PERSONNEL FAMILIAR WITH

LABORATORY PROCEDURES. THEY SHOULD ALSO BE FAMILIAR WITH THE NATURE

OF THE SUBSTANCES USED IN COMBINATION WITH THE INSTRUMENT AND ANY

NECESSARY PRECAUTIONS WHICH SHOULD BE TAKEN IN THE HANDLING, USE AND

STORAGE OF THE PRODUCTS IN NORMAL USE. THE CUSTOMER SHALL BE

RESPONSIBLE FOR THE CONTROL AND USE OF THIS INSTRUMENT WHETHER ALONE

OR IN COMBINATION WITH OTHER ARTICLES OR SUBSTANCES OR IN ANY OTHER

MANNER WHATSOEVER.

NOTE: Products and corporate names appearing in this manual may or may not be registered trademarks or copyrights of their respective companies, and are used only for identification or explanation and to the owner's benefit, without intent to infringe.

® Windows is a registered trademark of Microsoft Corporation.

® IBM is a registered trademark of International Business Machines.

® Intel and Pentium are registered trademarks of Intel Corporation.

® Lambda is an "Invensys Company."

® Pharmed is a registered trademark of Norton Performance Plastics Corporation.

® Baydur is a registered trademark of Bayer Polymers, LLC.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 7 of 12

CONFORMANCE

IMPORTANT NOTICE - EMC CERTIFICATE

The analyzer BT3000 PLUS has passed all the tests relevant to Electromagnetic

Compatibility (EMC) and electrical safety. This product received the certificate of compliance with international standards EN 66110-1 and EN 61326-1 from OCE

(Organismo di Certificazione Europea) of Rome (Italy), accredited and certified

(certificate number 051 of 21 Oct.1998) by Ministero delle Comunicazione of Italy.

The test reports 46/E and 47/E are available upon request.

This product conforms to the safety requirements of the Council Directives

98/79/EEC of 27 October 1998 (European Parliament) regarding the In-Vitro

Diagnostic Medical Devices. This directive is in accordance with the Article 2,

Paragraph 2 of the Directive 89/336/EEC, which ceases to apply to the products complying with the present directive. Refer to Paragraph 7, Article

No.1 of the IEC Official Gazette No. L331 of Dec. 1998.

It also conforms to Italian Regulations CEI EN 61010-01 and CEI EN 61326-

1 (EMC) .

The conformity is attested when the equipment is installed in accordance with the conditions outlined in the manual

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 8 of 12

THE COM PANY PROFILE

Biotecnica was founded in 1972, with years of technical expertise and manufacturing knowledge in the field of design, production, sales and technical support behind its staff. The Company develops and manufactures clinical chemistry equipment and relevant reagent products, to be sold directly and through OEM agreements. The market consists of private and state clinical laboratories, hospitals, clinics, day hospitals, and universities, etc. Since that time the company has developed and produced instruments of uncompromising performance characteristics, which has enabled Biotecnica to become one of Europe’s leading manufacturers of clinical chemistry instruments. Biotecnica is headquartered in Rome (Italy) and markets products both domestically and internationally through a network of distributors.

Our actual range of instruments is composed of:

• Photometers for clinical chemistry

• Automatic analyzers for clinical chemistry

• Electrophoresis densitometers & accessories

• Flame photometers & associated reagents

• Ion selective analyzer

• Clinical chemistry reagents

Biotecnica has over 3,000 square meters of manufacturing facility, which houses the latest in engineering, manufacturing, testing, and quality control equipment. It is staffed with highly experienced personnel with a dedication to quality.

The company supplies more than just products, it provides highly efficient technical and manufacturing support based on the requirements of its customers with ongoing training programs to keep them current on advancements in technology. A global network of distributors and sales engineers, assure the most comprehensive customer support across the world.

For more information about Biotecnica, visit the Biotecnica web site at www.biotecnica.it

Please do not hesitate to contact us. W e will be pleased to assist with any inquiries you may have.

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 9 of 12

PACKING / UNPACKING THE ANALYZER

UNPACKING THE ANALYZER & ACCESSORIES

Two wooden crates contain the analyzer and the accessories. The crates can be easily opened by applying a lever action, with a large screwdriver, to remove all the spring clips on the base of the crate as shown in the figure below. Carefully remove the upper covering from the base. Remove the analyzer and place it on a stable, vibration-free-surface, level table or cart. Carefully unpack all the accessories and place them in a protected place. Store the empty wooden crate assy in a safe place for future use.

Arrow Pointing

Upwards

Base

Spring Clip

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 10 of 12

Verify upon receipt of the BT3000 PLUS analyzer system that all parts are present and intact when opening the wooden crates and packaging. If there is a damage or missing items then please fill out the form found in the Operator's Manual.

Refer to Section 2, Chapter 2, and entitled "Warranty". Return it to your nearest sales/service office or directly to Biotecnica Instruments S.p.A. After appropriate evaluation, Biotecnica or its branch office will provide the best solution to the problem.

VERIFICATION OF THE CONTENTS OF THE WOODEN CRATES

In the Basic Package, the BT3000 PLUS analyzer system is provided with the following items:

Contents of Large Wooden Crate

Qty Description OK

1 ANALYZER

1

1

1

1

IMAGE DISK (HARD DISK)

WINDOWS 2000 PROFESSIONAL SOFTWARE DISK WITH LICENSE

DRIVERS AND UTILITY DISK

VGA DRIVER DISK

Contents of Small Wooden Crate

Qty Description OK

1

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

SURFACTANT WASH CONC. 2x50 ml (P/N 392)

WASHING SOLUTION FOR CUVETTE 1 liter (P/N 393)

ISE STARTER KIT (P/N 947)

Cl ELECTRODE CLEANING TOOL (P/N 03254)

VACUUM PUMP SYSTEM, (P/N 06-05161-01)

BIMONTHLY MAINTENANCE KIT (P/N 662.1016)

SIX-MONTHLY MAINTENANCE KIT (P/N 11-05219-01)

ANNUAL MAINTENANCE KIT (P/N 11-05220-01)

FUNNEL CAP OPENER, TOOL (90-05201-01)

TRANSPARENT OVERFLOW TUBE 50cm LONG

CORDLESS KEYBOARD & MOUSE (P/N 662.2057)

UPS UNIT 1100VA (P/N 330.2132)

PRINTER (P/N 330.2172)

FUSE 250 VOLT, 0.5A RVT (P/N 330.6338)

POWER CORDSET F/M VDE (P/N 330.6400) FOR PERIPHERAL DEVICES .

FUSES 250 VOLT, 8A RVT (P/N 330.6342B)

1

1

1

3

POWER CORDSET (P/N 330.6391).

QUARTZ HALOGEN LAMP 12V, 35W, 9° (P/N 330.9321)

CLEANING TOOL FOR SAMPLING NEEDLE (P/N 662.0629A)

CUBITAINER 10 LITERS WITH BOX (P/N 662.1010)

1 WASHING PISTON GRIP SLEEVE, TOOL (P/N 662.1025)

1,000 TRANSP. SAMPLE CAPSULE 2ml #STD & CTRL (P/N 667.1040)

50 REAGENT CONTAINER 80 ml WITH CAP (P/N 667.1072)

50 REAGENT CONTAINER 50 ml WITH CAP (P/N 667.1073)

25 REAGENT CONTAINER 20 ml WITH CAP (P/N 667.1074)

25 REAGENT CONTAINER 10 ml WITH CAP (P/N 667.1076)

1 50 ml BOTTLE WITH SCREW CAP (P/N 667.1080)

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 11 of 12

♦ VERIFYING EVENTUAL DAMAGES OCCURRED DURING SHIPMENT

It is highly recommended to accurately verify the instrument and its accessories for any damages that could have occurred during shipment. In case there is a damage or missing items then please fill out all the sections of the Form 05-35a in this section. Send it to your nearest sales/service office or directly to Biotecnica

Instruments S.p.A. Rome, Italy. After appropriate evaluation, Biotecnica or its branch office will provide the best solution to the problem.

NOTES FROM THE MANUFACTURER

The Biotecnica Instruments S.p.A. reserves the right to revise this manual without notice, for any reason. This includes but is not limited to, utilization of advance in the state-of-the-art and changes thereof. Product enhancement resulting from our continuing quality improvement effort may necessitate changes in specifications without notice. This fact doesn’t oblige the company to inform its actual customers because the information included in the present manual refers to state of the product when shipped, thus no warranty about notification of future changes is given.

The information contained in this manual is proprietary with

"Biotecnica Instruments S.p.A.". Reproduction of any part or whole may only be performed with written permission from

"Biotecnica Instruments S.p.A.".

Section A Warnings, Warranty, Unpacking BT3000 PLUS Rev.0, Soft Ver. 8 Page 12 of 12

SECTION - I

INTRODUCTION - BT3000 PLUS

The BT3000 PLUS is an automated analyzer for the determination of clinical chemistry and electrolyte results. The combined performance of this analyzer is from 300 to 500 tests per hour.

The technical material in this manual is for approved technical personnel with reasonable familiarity with similar instruments only. A diligent effort has been made to provide engineering illustrations, schematics, and other helpful information regarding the analyzer on the following pages to facilitate the task of service personnel.

The BT3000 PLUS is a fully automatic analyzer that can perform high volume of analysis on serum or urine samples. The test sample is followed continuously, upon insertion, so the analysis can be performed quickly and with maximum reliability. The automated instrument consists of the following three distinct subsystems: a) The Mechanical System: Utilized for the transfer of the solutions, samples, and reagents b) The Electronic System: Based on microprocessors for activating drive motors, alarm devices, and temperature control, etc. c) The Information Management System: IBM ® compatible for patient inputs, processing of the results, and communication with the host computer etc.

1-1. Examples of The Basic Operations of Clinical Chemistry

1-2. PHASE 1: Creation of the Worklist.

Section - I Introduction BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 3

1-3. PHASE 2: Placement of Samples and Reagents

1-4. PHASE 3: Start-up.

1-5. PHASE 4: Process Initiation

The mechanical arm is equipped with a sampling needle that can perform both swiveling and up/down motions to accomplish complete mixing during the sampling cycle along with the diluter pump. The cycle sequence starts with the aspiration of the reagent and the serum, followed by the dispensing and mixing in the reading cuvette.

The sampling cycle is performed using the following devices: a sampling arm, a diluter pump, and a peristaltic pump. The sequence of the sampling cycle is as follows: a) Washing and drying of cuvettes b) Reagent aspiration c) Needle washing d) Sample aspiration e) Dispensing and mixing the solution in the pre-washed and dried cuvette f) Washing the needle and the hydraulic circuit

The diluter provides the aspiration of the solution. A built-in liquid sensor, attached directly to the needle mechanism, controls the descent of the needle into the reagent or sample. This controlled immersion of the needle avoids any excessive contamination. The first part of the hydraulic circuit (located inside the sampling arm) is pre-heated to about 40°C in order to compensate for the low temperature of the reagent. The peristaltic pump provides approximately 2.0ml of H

2

O into the waste bowl to wash the needle and the attached hydraulic circuit.

Section - I Introduction BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 3

1-6. PHASE 5 - Reading Cycle

In this phase, the sample remains in its cuvette for the necessary incubation time, after which, the reading phase will begin. The reading cycle takes place every 10 seconds and lasts about 2 seconds. The photometer, located under the reading station, converts the luminous intensity of the cuvettes into digital signals and transfers them to the microcontroller. After the initial reading, the acquired data is stored as concentration and absorbance values for generating the test results.

1-7. Examples of Basic Electrolytes Operation

The process described above regards the complete dynamics for the testing of clinical chemistry samples. The operation for running electrolytes is similar and can be performed simultaneously with the preceding clinical chemistry samples.

The final solution to be analyzed is not dispensed into a cuvette, but into the ISE

(Ion Selective Electrode) funnel located on top of the ISE module. Obviously the phases 1, 2, and 3 are basically the same.

The right sampling arm aspirates the reagents positioned near the ISE Module on the right of the analyzer. The sampling is performed in two phases. The first phase is dedicated to the aspiration and dispensation of the reference solution into the

ISE funnel. The second phase is assigned to the sampling of serum aspirated together with diluent reagent and buffer. The solutions are dispensed and mixed in the ISE funnel followed by the use of a peristaltic pump to draw the solution into the ISE electrodes for measurements. The signal produced by the electrodes is then processed, as per the NERNST formula, and is translated into values of concentration. The ISE module has its own diluter pump that is similar to that of the sampling (located on the reading station) for clinical chemistry.

Section - I Introduction BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 3

SECTION - II

TECHNICAL SPECIFICATIONS

NOTE: Specifications are subject to change without notice.

2-1. PHYSICAL DIMENSIONS OF THE BT3000 PLUS

Packed analyzer without accessories:

Packed analyzer with accessories:

Packed accessories:

Analyzer dimensions:

Crate dimensions:

Accessories package dimensions:

Approximately 135 Kg. (298 lb)

Approximately 225 Kg. (496 lb)

Approximately 90 Kg. (199 lb)

W = 100cm (40"), D = 58cm (23"), H = 68cm (27")

W = 120cm (48"), D = 80cm (32"), H = 80cm (32")

W = 120cm (48"), D = 72cm (29"), H = 70cm (28")

NOTE: Weight and dimensions shown are approximate.

2-2. POWER SUPPLY REQUIREMENTS

BT3000 PLUS analyzer:

Input Voltage Range: Universal input, 90 - 240 V~

Power Consumption: 590 Watts max

New "Vacuum Pump System" P/N 06-05161-01:

Power: 60 Watts

Previous "Vacuum Pump System" P/N 662.0788 - (OBSOLETE)

Input: 220 V~, 50-60 Hz (110 V~ on request)

Power Consumption: 80 Watt

Printer (accessory):

UPS (optional):

Approx. 50 Watts, 100/240V~ (depending upon model)

Input 210V~, 50-60 Hz (110 V~ on request)

Output 210V~, 50-60 Hz (110 V~ on request)

The detailed information regarding the specifications of the instrument power supply is outlined in Section XI entitled "Power Supply" The internal power supply of the analyzer is stabilized and incorporates the Power Factor Correction device (PFC), thereby require no additional external electronics accessory. The use of an optional UPS device

(uninterrupted power supply) is recommended to avoid undesired power interruptions and to ensure software integrity in case of sudden power failure.

2-3. EMC AND ELECTRICAL SAFETY

The BT3000 PLUS chemistry analyzer conforms to following EMC directives (Electro

Magnetic Compatibility):

EN61326

EMC:

Electrical Safety: EN61010-1

The EMC performance characteristics and electrical safety reports are available upon request.

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 6

2-4. PERFORMANCE & TECHNICAL SPECS

Operating Mode:

Methods:

ISE Module:

Test Mode:

Tests on line:

Tests in memory:

Test Re-runs:

Calibrations and Controls:

Automatic Profiles:

Measurement:

Quality Control:

Remote Diagnostic:

Random access

Tests for Clinical Chemistry and Immune-Chemistry

Na, K, Cl (CO

2

optional)

Routine, Batch, Emergencies (STAT), and profiles

80 refrigerated reagents + Relation Tests

500 single or double- reagent + unlimited Relation Tests

Automatic or on demand

Automatic or on demand

Automatic execution of related profiles or on demand

Direct reading of 34 cuvettes in non-disposable optical glass

3 known levels and 3 unknown levels

Via modem (optional)

Maintenance: Automatic

Sample Tray Capacity:

Sampling Arm:

52 positions for Samples & STAT, 26 for Standard and

Controls

2, for serum and reagents

Bar code scanner: 2 distinct readers for positive identification of samples and reagents

TIME REQUIRED TO REACH STEADY STATE

Ambient conditions:

Time required for the analyzer to reach steady state:

Ambient conditions:

Time required for refrigerated bottles to reach steady state:

21 °C R.T., 33% RH

20 minutes

21°C T. A., 33% RH

Approximately 2 hours

CLINICAL CHEMISTRY

Sampling Cycle:

Analytical throughput:

Test processing time

11 seconds

330 tests/hour single reagent max 360 seconds

ISE PROCEDURE

ISE Test processing time:

Executive throughput:

Analytical throughput:

70 seconds

51 Samples/hour

204 Results/h (K + , Na + , Cl and CO

2

)

2-4.1. OPERATING AMBIENT CONDITIONS

The instrument will perform optimally in the following ambient conditions:

CUVETTE OPERATING TEMPERATURE

Programmable Temperatures: Room Temperature, 32 °C, and 37 °C

Precision ± 0,2°C - Accuracy ± 0,2°C

Temperature Monitoring Device based on Peltier Effect

REAGENT CHAMBER TEMPERATURE

Nominal Temperature: 11°C Approx.

Temperature Monitoring Device based on Peltier Effect

OPERATING AMBIENT TEMPERATURE

18 °C to 32 °C, 10% to 90% RH, Non condensating

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 6

2-4.2. VOLUMES

WORKING SOLUTIONS

Clinical Chemistry

Reaction Volume:

Sample Volume:

280 µl minimum - 700 µl max (for double reagent)

From 1 to 100 µl

ISE

Concentrated Buffer Solution:

Concentrated Reference Solution:

Sample Volume:

Concentrated Buffer 10 ml:

50 µl + 400 µl (H

50 µl + 400 µl (H

30 µl

200 tests

Concentrated Reference Solution 10 ml: 200 tests

2

2

O)

O)

RESIDUAL VOLUMES OF REAGENT BOTTLES

80 ml BOTTLES:

50 ml BOTTLES:

20 ml BOTTLES:

10 ml BOTTLES:

1 ml

1 ml

0.5 ml

0.5 ml

The ISE Module is designed to utilize solid state Ion Selective Electrodes:

Number of electrodes:

Analytes: K

Test processing Time for the ISE:

Executive throughput:

Analytical throughput:

4 plus Reference Electrode

+

, Na

+

, Cl

-

e CO

2

70 seconds

51 Samples/h

204 Results/h (K +

(optional)

, Na + , Cl -

Number of Reagents for the test:

Sample Type:

Minimum Sample Volume:

Concentrated Reagents Dilution:

and CO

2

)

2, Buffer and Reference Solution (concentrated)

Serum, Urine (Whole)

30 µl

1/10 with H

2

O (automatically)

1/14 (automatically)

+

Dilution of Serum/ Buffer:

Precision on Serum test:

Precision on Urine test:

Linearity for Serum test:

± 1% for K

+

, Na

+/- 2% Cl

-

+/- 3% CO

+

2

K

+

, Na

50 - 200 mEq/l

+

,

Cl

-

Cl

Linearity for Urine test:

+/- 2%

Na

-

Na

50 - 400 mEq/l

+ 20 - 400 mEq/l

Cl

-

40 - 400 mEq/l

Accuracy for Serum test:

Intra Run Serum (20 samples): Na

Cl

-

+

K

+

C. V. < 1%

C. V. < 2%

K

K

+

+

1 - 20 mEq/l

CO

2

10 - 45 mEq/l

2 - 200 mEq/l

CO

Infra Run Serum (N°20 samples): Na

Cl

e K

CO

2

Accuracy for Urine test:

Intra Run Urine (20 samples): Na

+

, K

+ e Cl

-

CO

Infra Run Urine (20 samples):

Average Life of Electrodes:

Na

Na

K

+

Cl

-

+

2

+

+

, K

C. V. < 5%

C. V. < 2%

C. V. < 2.5%

C. V. < 5% e Cl -

C. V. < 2%

C. V. < 5%

C. V. < 2.5% and Reference Electrode 12 months

& CO

2

3 months

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 6

2-4.4. “UTILITY” EXECUTION TIMES

Wash & Fill Up:

Wash H O:

2

Zeroing:

F.C.C.:

Extra Wash ISE:

Extra Wash Cuvette:

Wash Shut Down:

WASH VOLUMES

Clinical Chemistry Washes:

H

2

H

2

O Single Wash of Cuvette:

O Single Wash of Needle:

Consumption per test:

270 sec. (approximately)

180 sec. (approximately)

420 sec. (approximately)

900 sec. (approximately)

800 sec. (approximately)

540 sec. (approximately)

12 min. (approximately)

5 ml (approximately)

2 ml (approximately)

7 ml (approximately)

H

2

O for Zeroing:

H

2

O Total Wash of Cuvette:

Extra Wash of Cuvettes:

250 ml (approximately)

170 ml (approximately) approx. 170ml H

2

O + 13ml Cuvettes Washing Solution

I.S.E. Washes:

H

2

O per test:

I.S.E. Extra Wash:

2 ml (approximately)

0.5 ml I.S.E. Washing Solution

0.5 ml Enzyme Solution

2-5. FUNCTIONAL LIMITS

The instrument will not perform correctly in the following situations:

1) Out of range (defined) ambient conditions.

2) Use of unapproved chemical products such as Washing Solution, Distilled Water, and ISE reagents etc.

3) Non-observance of care and maintenance procedures.

4) Use of unapproved spare parts and consumables.

The manufacturer does not guarantee the correct instrument performance if new methods are implemented unexpectedly. Consult the manufacturer regarding application of different methods.

2-6. CUVETTE TEMPERATURE CONTROL

The cuvette plate is thermally controlled in a very precise way. The solution dispensed in the cuvette reaches the working temperature in minimal time possible. However, it is possible to observe a notable time difference due to the potential difference of the temperature of reagents. Therefore, the heated sampling tube aids in preheating the reagent before dispensing.

The stabilization times of the solution in cuvette range from 80 seconds to 60 seconds with reagent temperatures at 11°C and 22°C, respectively. The time was measured after the solution had reached 36.7°C:

Accuracy measured after 2 minutes:

Adjustment measured after 2 minutes:

+/- 0.25°C.

+/- 0.20°C.

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 6

2-7. REAGENT REFRIGERATION

The purpose of reagent refrigeration is for optimal conservation and reagent performance. The temperature inside the reagent chamber is variable, based on the external ambience and the time inside the refrigerated chamber. A reagent bottle inside the chamber gradually reaches the minimum temperature within 2 hours. The temperature range may go from 10°C to 22°C with outside temperature of 20°C and

32°C, respectively.

2-8. DILUTER LINEARITY AND ACCURACY

A unique diluter system is used for the processing of chemistries and ISE testing.

Technical Specifications for the Biotecnica dilutor are as follows:

Diluter:-

Linearity F.S.:

Accuracy at 3 µl:

+/- 0.1% F.S.

+/- 3%

Accuracy from 10 to 470µl: +/- 1%

Reproducibility: +/- 0.7% at 3µl; +/- 0.6% > 3µl

Life Expectancy: 3 million operating cycles

Maintenance: Every 300,000 operating cycles (O-ring seal replacement)

Peristaltic Pump:-

Dispensation Volume:

Average Life

Maintenance:

Approximately 1ml per sec.

>1 million cycles

Every 50,000 cycles (Cartridge replacement)

Pinch-Valve:-

Average Life:

Maintenance:

Approximately 1 million cycles

Monthly replacement of valve tube

NOTE:

All declared values are approximate and are dependent upon work load of the components, inactivity, and observance of routine maintenance.

2-9. PHOTOMETER LINEARITY AND ACCURACY

Optical System Solid state photometry, (patented by Biotecnica Instruments)

Detectors 10 UV/VIS photodiodes + reference channel

PRECISION AND ACCURACY

Spectral Response: 340, 380, 405, 436, 480, 510, 546 578, 630, 700 nm

+/- max

Linearity O.D.: +/- 1% from 0 to 2,000; +/- 2.5% from 2,000 to 2.400

Drift: +0.005 Abs. per hour (after warm-up)

Optical path length 7 mm

NOTE: The specification regarding drift applies exclusively to the photometer, excluding the drift related to the halogen lamp of the photometer.

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 6

2-10. PHOTOMETER TUNGSTEN HALOGEN LAMP

This lamp is custom made for Biotecnica Instruments S.p.A. It is a halogen lamp with dichroic reflector and a special coating for maximum UV (ultra violet) emission.

Angle:

Watts:

Volts:

Avg Rated Life:

12

Approximately 2000 hours

NOTE: For optimal result the lamp can be used for about 1500 hours. The long-term use will result in the gradual deterioration of the UV emission.

> Pentium IV > 2400 MHz or more, IBM compatible

DVD/CD Rom Player

>20

Floppy Disk Drive

> 16X

Gb

1,44 Mb

Monitor

Interface

Printer

Mouse & keyboard

Modem

Error Messages

LCD Display Module TFT 12” with integrated touchscreen

2 Serial Ports RS232C + 2 USB PORTS

Ink-jet Color IBM compatible or other

Cordless

Visible (Vocal optional)

Mixing, Prime, Water H

2

O Supply, Liquid Sensor Sensitivity, Contamination, Sample

Viscosity, Carry-Over, and Sample Evaporation.

2-13. WASHING SOLUTION SUPPLY

The analyzer BT 3000 PLUS, utilizes distilled water mixed with a surface - active agent

(surfactant) for all the washing and sampling operations. The operator can use any container of desired dimensions for the washing solution. The container can be positioned at a considerable distance from the analyzer as a powerful peristaltic pump is used for constant supply of washing solution to the additional reservoir of the analyzer.

The maximum permissible connecting tube distance between the analyzer and the water container is 25 meters horizontally and 6 meters vertically. This data is valid for a flexible plastic tube with inside diameter of 6 mm max.

The liquid sensor incorporates a high frequency electronic circuit with functions of capacitive discriminator.

For this characteristic, the sensor can detect any type of liquid including distilled water with the same sensitivity. In the normal operating conditions the minimum detectable volume is 100 µl.

The limiting factor necessitates that the container is surrounded by a metallic surface.

The distance of the isolating material from the metal should not be more than 4 mm.

However in different conditions and without the surrounding metallic material around, the analyzer is sensitive to minimum volumes of 150 µl.

Recently carbon filled plastic adapters (black) have been introduced, which have identical characteristics to the ones in metal.

Section - II Technical Specs BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 6

SECTION - III

ELECTRONICS

3-1. Top View of the BT3000 PLUS

Section - III Electronics BT3000 PLUS Rev.0, Soft Ver. 8 Page 1 of 5

The analyzer structure is composed of a heavy-duty sheet metal. The outer casing and covers are molded in a highly resistant Baydur ® material. The preceding figure illustrates the location of various modules on the analyzer. Each module has a specific function for the analyzer operation.

3-3.1. 3-3.4.

3-3.2.

3-3.5.

3-3.7.

3-3.3.

3-3.6.

3-3. Description of Analyzer Modules

The computer module consists of a LCD monitor, a touch-screen, main-board, power supply, and peripheral devices.

Section - III Electronics BT3000 PLUS Rev.0, Soft Ver. 8 Page 2 of 5

The reading station is comprised of a cuvette plate, photometer, diluter, washing station, water reservoir, and the associated electronics.

3-3.3. Power Supply Module

The power supply module is the general power source of the analyzer.

The reagent system consists of the rotating reagent plate, refrigeration system, barcode, and electronics.

The sampling system consists of the rotating serum plate, barcode, Vacutainer ® sensors, washing bowls, and electronics.

The ISE module consists of the electrodes panel, diluter, ISE reagents, and electronics.

3-3.7. Dual Sample Arm Modules

The dual sample arm modules consists of 2 sampling arms, incorporating electronics with appropriate Servomotors, and sampling needles.

Note: For detailed description of the preceding modules, refer to Sections VI to XII of this manual.

Section - III Electronics BT3000 PLUS Rev.0, Soft Ver. 8 Page 3 of 5

3-4. System Electronics

The electronics of the analyzer, with the exception of the IBM compatible computer, is composed of 80C51 micro-controllers. All the modules are defined as intelligent peripherals.

Micro-controller 87LPC764 is programmed to perform process control functions assigned to it as a slave driver. The master functions are performed by a 552 microprocessor, which communicates with the main-board of the IBM through RS

232 serial port. The modules communicate between them through the I 2 CBUS protocol, established by only two conductors, SCL and SDATA. The wiring of the instrument is extremely simplified, with the cable connecting various modules has only two wires for communication and additional wires for power supplies.

The power supply of the instrument is composed of two power supplies of high quality. One power supply is located inside the computer module and is used for powering the IBM module with its various peripheral devices. The 5 Volt power supply, in addition to supplying the main-board, is used for energizing the series of micro-controller modules.

The second power supply, located outside the computer module, is for the supply of voltage to the power circuits. The three voltage outputs power the following systems:

1. 24V, 8A; variable from 18 to 26 Volts, for refrigerated Peltier groups.

2. 24V, 8A; stabilized the servomotors, solenoid valves, pumps, temperature controls, and fans

3. 12V, 8A; stabilized, for Halogen lamp of photometer

All of the supplies are isolated to enhance digital communication through the optoelectronic devices, thus greatly reducing the interference, which is typical of direct digital-power circuits communication. Both of the power supplies incorporate PFC and have a universal input, which ranges from 100 to 240 Volts.

Section - III Electronics BT3000 PLUS Rev.0, Soft Ver. 8 Page 4 of 5

3-5. Example of the I 2 C-BUS Configuration

The I 2 C-BUS uses several microprocessors as shown below:

VDD

SDA

SCL

RST

RP RP

Microprocessor

Master Logic

Microprocessor

Slave Logic

(Peripheral)

Microprocessor

Slave Logic

(Peripheral)

Microprocessor

Slave Logic

(Peripheral)

Section - III Electronics BT3000 PLUS Rev.0, Soft Ver. 8 Page 5 of 5

SECTION - IV

SOFTWARE

Important Notice:

Any modification to the Variable Serial Protocol is restricted to qualified personnel only.

The Biotecnica Instruments S.p.A. guaranties the correct performance of the internal serial protocol. The responsibility for any malfunction arising out of any modifications to the scripts of the Variable Serial Protocol rests with the customer.

WARNING

This information regards the setting up of the barcode for sample tubes identification. The reading of the sample barcode label has the same progression as patient code.

For example: Once a patient code of 15 characters has been entered, then a code of 8 characters followed by 7 empty spaces to reach the 15 characters is sent.

The code read on the barcode label must have the same sequence 8 + 7 for correct detection.

SERIAL COMMUNICATION OF THE BT3000 PLUS

TO A HOST COMPUTER

4-1. GENERAL

The analyzer BT 3000 PLUS allows bidirectional communication through RS 232C serial connection with any host computer.

The particular feature of the dialog is that it is always the host computer, which initiates the communication for either transmitting patient list or for receiving the results.

To initiate any communication the host computer will have to send to analyzer the character STX (0x02) and expect the character ACK (0x06) as a response. At this point the host computer will send data to the analyzer and terminate the communication by sending the character EOT (0x04).

It is important to remember that any communication is followed by a response from the analyzer.

It must be noted that if the parameter to be transmitted is shorter in length than the length requirement of the communication protocol than a space must be added before or after.

For example the analysis have length 4, therefore to send a code GLI one must add a space before or after to reach the length of 4 characters.

4-2. PATIENT

► Start communication with sequence STX<->ACK

► Send patient code................................................. (15 characters)

► Send list type for patient insertion......................... ("T" for Routine or "R" for STAT)

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 1 of 15

► Send type of serum............................................... ("S" for Serum or "U" for Urine)

► Send if the patient is a clone................................. ("Y" for Yes or "N" for No)

► Transmit position of capsule................................. ("00" unknown)

► Send number of tests to be executed................... (from "01" to "99")

► Send codes of tests to be performed.................... (4 characters)

► Send Check-Sum.................................................. (3 characters)

► Send end transmission character EOT

► Wait for response from the analyzer..................... (2 characters)

If the communication is successful then the analyzer responds with character "Y" followed by a byte, which identifies the position where patient has been inserted. In case the communication was unsuccessful, then the analyzer responds with "N" followed by a byte identifying the type of error. The possible errors generated by the analyzer in response to the invalid insertion of patient are as follows:

0x01........... Check-Sum Error

0x02........... Unknown Command

0x03........... Routine/STAT field Error

0x04........... Serum/Urine field Error

0x05........... Clone Yes/No field Error

0x06........... Capsule position Error

0x07........... Number of Analysis field Error

0x08........... Wrong Number of Test

0x09........... Position already in execution

0x0A.......... Cloning impossible

0x0B.......... Code duplicated

0x0C.......... One or more analysis not present in the analyzer

0x0D.......... One or more analysis not present in the current plate

0x0E.......... Too many analysis for the patient

For example to send a patient with code 000000000000001, serum type and with analysis GLI, BUN and COL onto the STATS list, then one must send the following sequence of characters (excluding initial sequence STX<->ACK):

000000000000001RSN0003GLI BUN COL 134<EOT>

Where:

000000000000001...... Patient code

R................................. Identifies STATS list

S................................. Identifies the type of patient (in this case: Serum)

N................................. Identifies that the patient is not a clone

00................................ Unknown position (the analyzer will insert the patient in a convenient position)

03................................ identifies the number of test to be executed.

GLI, BUN, COL.......... Test codes (observe the space after each code to reach the 4 characters limit)

134.............................. Identifies the Check-Sum

<EOT>........................ This character ends communication

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 2 of 15

There are three commands for receiving reports from the analyzer:

R........ Reception of next available report

L......... Reception of the last report sent (in case of reception problems)

A........ Reception of the first available report (in case one desires to receive again all the reports)

The commands R, L, and A require standard communication or the procedure STX<-

>ACK and the character EOT to end communication.

As a response to one of these three commands the analyzer sends the requested report

(if available) or the character NAK (0x15) if there is no report to be sent.

It must be borne in mind that after a run test the reports are not immediately available for transmission, as these need validation. To do this go to Utility menu, RS232 and enable the option "Accept result to be sent". This operation must always be performed after each run test or groups of run test.

There is also an additional option for performing validation operation automatically. Go to

Setup of the analyzer (Menu Utility, Setup Analyzer), go to the Serial (fourth from the left) and enable the option "All results must be sent automatically (without validate)" at the bottom of the page.

In case of positive response to the request for a report the analyzer transmits:

Patient code.................

List type.......................

Sample type.................

Number of reports.......

15 characters

"T" for Routine or "R" for STATS

"S" for Serum or "U" for Urine

3 characters

For each report:

Analysis code..............

Result...........................

Check-Sum..................

<EOT>

4 characters

7 characters

3 characters

The following is an example of eventual response to the data sent in "Sending a patient to

BT 3000 PLUS":

000000000000001RS003GLI 000.000BUN 0010.10COL 00100.0107<EOT>

Where:

000000000000001..

R.............................

S..............................

003..........................

GLI..........................

000.000...................

Patient code

Identifies STATS list

Identifies the type of patient (in this case: Serum)

Identifies numbers of reports

First test code

GLI test result

BUN........................ Second test code

0010.10................... BUN test result

COL........................ Third test code

00100.0................... COL test result

107.......................... Identifies the Check-Sum

<EOT>.................... This character ends communication

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 3 of 15

4-4. CALCULATION OF CHECK-SUM:

This procedure calculates a Control code in accordance with the transmitted or received data.

An algebraic sum of ASCII values of all the sent characters is executed. For example the character "A" has ASCII value 65 - 0x41.

Consequently the module 256 of the found value is executed (balance of dividing the value by 256). This is the Check-Sum to be sent.

4-4.1. ADDITIONAL NOTES ON THE SERIAL PROTOCOL

The patients reception has been improved in the following manner:

1) If a patient is already in the list and is sent again, then the same will be overwritten.

2)

3)

If more patients above the real capacity of the list were sent then these were sent to the supplementary list of controls. This error has been corrected.

The following errors have been added to the existing list:

Code Description

22 A patient has been sent after assigning him a specific position, which is already occupied.

23 The patient is already in the list (or has been executed), the Clone field has not been enabled, but the list of assignment is different in comparison to that of the patient stored in the analyzer.

24 The transmitted code belongs to an executed patient but neither the Repetition field nor the Clone field has been activated.

4-5. WIRING DIAGRAM OF INTERFACE CABLE

BT3000 PLUS

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 4 of 15

BT3000 PLUS

4-6. VARIABLE SERIAL PROTOCOL

Introduction

The variable serial protocol has been designed to provide the user with possibility to personalize the transmitted and received data from the analyzer.

The user can transmit or receive in addition to preset data (patient code, analysis code, results etc.), also the simple text strings and/or characters in order to meet the personal requirements.

Not only the user can decide to send or receive numerical information (for example number of tests) not as single byte but as a preset numerical string or vice versa.

For example the user can decide to receive something like:

"Initiate analysis data"

<Analysis data true and typical>

"End analysis data"

Where the phrases "Initiate analysis data" and "End analysis data" do not refer to any preset data by the analyzer but serve only for monitoring communication process (can be useful for inserting specific markers on those programs which obtain information from text files).

It is obvious that the protocol of initiation and end of communication, the commands for the request of report, and the analyzer responses in case of error or success remain identical to the usual preset serial communication.

NOTE: a) If a check-sum is omitted in a communication then the analyzer will not control it. b) The following numbers have been used to represent the error codes relevant to sending a patient to the analyzer as regards the parameters not part of the standard serial communication:

Error Code

1

Description

Number of invalid analysis

2

3

4

Invalid Data (not through variables)

Invalid Data (through variables)

An analysis variable is outside the SET BEGIN/END relative to the analysis

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 5 of 15

Error Code

18

19

20

21

22

23

24

Description

The patient is not to be repeated

The field corresponding to the Serum/Urine is different from the patient in memory

Patients list full

Transmitted list different from the original

A patient has been sent after assigning him a specific position, which is already occupied.

The patient is already in the list (or has been executed), the

Clone field has not been enabled, but the list of assignment is different in comparison to that of the patient stored in the analyzer.

The transmitted code belongs to an executed patient but neither the Repetition field nor the Clone field has been activated.

Notes regarding Scripts

A script is a text document. Each one of the single commands must each reside in a different line and be complete. In other words a single command cannot be divided into more lines.

Stringn ‘Name’|$10

Stringn ‘Name’|$10 char ‘A’

Stringn ‘Name’

|$10

-> Valid line

-> Invalid line

-> Invalid Command

An editor for writing, modifications, saving and compiling of one or more scripts is accessible inside the program (setup function). In any case it is possible to write a script with any text editor (DOS or Windows) like Notepad of Windows or the EDITOR of the

DOS. It is not possible to import documents written with UNIX as the characters used for going to the next line are different from the ones used by the DOS or Windows.

CAUTION!

If one wants to use the script stored in a removable disk (for example floppy disk) then it will be necessary to copy it on the hard disk.

TYPE OF DATA

Character: Identifies a single character, can pass as printable character (enclosed between single apostrophes), as decimal ASCII value (followed by symbol $) or else hexadecimal ASCII value (followed by 0x).

If for example we want to identify the character A (decimal value 65 or hexadecimal value 41) then we can write 'A', $65 or 0x41.

String: Identifies a sequence of printable characters enclosed in single apostrophes, for example: 'this is a string'.

Comment: Identifies a portion of test (preceded by a character ; which will not be compiled but will serve as note only for the programmer.

Variables: These are particular sequence of characters preceded by the symbol

#, which will be used by the program for storing internal informations

(patient code, analysis name and etc.), refer to "TABLE 1 -

TRANSMISSION/RECEPTION".

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 6 of 15

There are also variables for direct uses, which allow for identification of any character below ASCII 32 (space) to facilitate the writing of the script (for example, one can use the variable #EOT to identify the character $4), see "TABLE 2 - INTERNAL VARIABLES".

SCRIPT FUNCTIONS

String: Identifies a string of variable length ending with a particular character.

Syntax:

String <string>I<Terminator>

Where

<String>

Note:

It is not possible to use the variables like parameter <Terminator>

Example:

String ‘Hello Word’|$0

String ‘My String’|’@’

String #Variable1|0x10

Stringn: Identifies a string of fixed length

Syntax:

Stringn <String>|<Length>

Where

Note:

If the length of the text strings is less than the data length then a series of spaces will be added on the right to reach the data preset length. In case the text string is longer than the data length then the string end will be cut off to match the data length.

If the length of the numerical values is less than the data length then a series of characters '0' will be added to the left to reach the preset data dimension. In case the length of the numerical values string is longer than the data length then the string will be truncated to match the data length.

It is not possible to use variables as parameter <Length>.

Example:

Stringn ‘Hello Word’|$40

Stringn #Variable1|0x10

Char: Identifies a single character (or single byte)

Syntax:

Char <Character>

Example:

Char ‘H’

Char $20

Char 0x10

Char #STX

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 7 of 15

Set: Identifies the beginning and the end of the group of repetitive commands

Syntax

Set Begin<Name of group>

Begin repetitive group

Set End<Name of group>

End repetitive group

NOTE:

Actually the ANALYSESDATA is the unique SET present, which identifies the analysis in transmission/reception.

Only one command SET BEGIN and one command SET END can be present in a script.

A script must always contain the command SET.

The variable PATIENTNUMBERTEST must be present before the command SET.

COMPILATION ERROR

One or more errors due to incorrect script writing or the system error may show up during compilation of a script. The compiler shows the error code, the description of error, and the line where it has been detected.

The following table shows the error codes, description, and the possible causes:

Error Code

2

3

04

6

7

8

9

12

Description

A string request

A number request

Invalid number format

Possible Causes

An invalid command has been inserted in the commands of script.

A string as first parameter for String or Stringn command has not been inserted.

A string like parameter <lunghezza> of command

Stringn has been inserted.

Inserted invalid decimal or hexadecimal number. a) Inserted more than two parameters for command String or Stringn. b) Inserted more than one parameter for the command Char or Set.

Invalid data A string for command Char has been inserted.

String Terminator Request The end (') character of a string not found.

Too little data

Invalid String Length a) Inserted less than two parameters for command String or Stringn. b) No parameter inserted for command Char or

Set.

The string length for Stringn command is less than 0 or more than 128. a) An empty string inserted for the command

Damaged file b)

String or Stringn.

Inserted a character identified as " a) Tried to transfer an invalid variable in the list of internal variables. b) Tried to use a transmission variable in the script of reception or vice versa.

Hard disk error. Contact Sales/Service.

Internal error. Probably damaged program. Reinstall the program. If the problem persists contact sales/service.

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 8 of 15

14

15

16

17

18

19

20

21 a) The text SET BEGIN or SET END not written.

Incorrect identifier in the

SET command. b) A different value from ANALYSEDATA transferred as <Group name> for the SET command.

Damaged exit file Hard disk error. Contact sales/service.

SET command not closed The SET END not inserted in the script.

Too many SET commands More than one SET BEGIN command inserted.

SET command not found The SET BEGIN command not found in the script.

Incorrect variable for SET command

A different value from ANALYSEDATA transferred as <Group name> for the SET command.

Variable not found before The highlighted variable required in the script before the SET command the SET BEGIN command.

The variable must be String type

The highlighted variable must be String type, not

Char

TABEL 1 – TRANSMISSION

The following variables are used for the transmission of a report from analyzer to the host computer:

Variable Usage

PATIENTCODE

PATIENTNAME

PATIENTSURNAME

PATIENTGROUP

PATIENTLISTTYPE

PATIENTTYPE

PATIENTNOTE

PATIENTNUMBERTEST

CHECKSUM

ANALYSESCODE

ANALYSENAME

ANALYSESTYPE

ANALYSESCONCENTRATION1

ANALYSESCONCENTRATION2

ANALYSESFLAGS1

ANALYSESFLAGS2

ANALYSESMINVALUE

ANALYSESMAXVALUE

ANALYSESUM1

ANALYSESUM2

ANALYSESUMFACTOR

ANALYSES2RESULT

ANALYSESSERUMTYPE

ANALYSESURINE24H

Patient Code

Patient Name

Patient Surname

Group

(1)

List

(2)

Method Type

(3)

Descriptive Note

Number of Results

Check-Sum

Analysis Code

Analysis name

Analysis Type (4)

1st Concentration

2nd Concentration

Flag 1st Result

Flag 2nd Result

Minimum Value

Maximum Value

1st Unit of Measurement

2nd unit of measurement

Unit Factor

Does the 2nd result exists?

(5)

Method Type

(3)

Urine in 24/h

(1)

Identifies Male, Female or Child (Select one of these):

‘M’ : Male

‘F’ : Female

‘C’ : Child

(2)

Identifies Routine or STAT (Select one of these):

‘R’ : Routine

‘S’ : STAT

Transmitting patient from archive will always have identifier of Routine.

(3)

Identifies Serum or Urine (Select one of these):

‘S’ : Serum

‘U’ : Urine

Type of valid data

String

String

String

String Character

String Character

String Character

String

String Character

String Character

String

String

String Character

String

String

String

String

String

String

String

String

String

String Character

String Character

String

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 9 of 15

(4)

Identifies Clinical Chemistry, ISE Module or Relation Analysis (Select one of these):

‘C’ : Clinical Chemistry

‘I’ : ISE Module

‘R’ : Relation Analysis

(5)

Identifies if the 2nd result exists or not (Select one of these):

‘Y’ : 2nd result exists

‘N’ : 2nd result does not exists

• If only the final result is desired then always refer to variables pertaining to 2nd result.

• In case of the absence of 2nd result then its variables will have the same values of the 1st result.

TABLE 1 – RECEPTION

The following variables are used for reception of a patient by the analyzer:

Variable

PATIENTCODE

PATIENTNAME

PATIENTSURNAME

PATIENTLISTTYPE

PATIENTGROUP

PATIENTTYPE

PATIENTURINE24H

PATIENTNOTE

PATIENTISCONTROL

PATIENTCONTROLKNOK

PATIENTCONTROLLEVEL

PATIENTCLONE

PATIENTCUPPOSITION

PATIENTNUMBERTEST

#PATIENTTOREPEAT

CHECKSUM

ANALYSESCODE

Usage

Patient Code

Patient Name

Patient Surname

List

(1)

Group (2)

Method Type

(3)

Urine in 24/h

Descriptive Note

If the patient is a control (4)

If it is a known control

(5)

Control Level

(6)

If it is a clone

(7)

Vial (Cup) position

Number of test

Patient repetition

Check-Sum

Analysis Code

Type of valid data

String

String

String

String Character

String Character

String Character

String

String

String Character

String Character

String Character

String Character

String Character

String Character

String Character

String Character

String

(1)

Identifies Routine or STAT (Select only one of these):

Routine

STAT

Routine

‘1’ :

‘R’

STAT

:

‘STAT

(2)

STAT

Routine

:

Identifies Male, Female or Child (Select only one of these):

Male

Female

$2 :

Male

‘1’ :

‘M’

‘F’ :

‘C’

Child

:

Female

:

‘MAN’

‘FEMALE’ :

‘CHILD ’

Male

Female

:

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 10 of 15

(3)

Identifies Serum or Urine (Select only one of these):

SERUM

URINE

‘0’ :

URINE

‘S’ :

‘U’ :

‘SERUM ’

‘URINE’

: SERUM

: URINE

(4)

Identifies a Control or a Sample (Select only one of these):

Sample

Control

‘N’

Sample

‘1’ :

Sample

‘Y’ :

‘C’ :

‘NO’

Sample

Control

:

‘YES’ Control

‘SAMPLE’ :

Control

(5)

Identifies a Known or Unknown Control (Select only one of these):

Unknown

Known

‘N’

‘U’

Unknown

‘1’ :

Unknown

‘Y’ :

Unknown

‘K’ :

‘NO’

‘YES’

Unknown

Known

‘UNKNOW’ :

‘KNOW’ : Known

(6)

Identifies Control Level (Select only one of these):

$1

$2

: Level 1

: Level 2

$3

‘1’

‘2’

‘3’

‘L’

‘N’

‘A’

‘LOW’

: Level 3

: Level 1

: Level 2

: Level 3

: Level 1

: Level 2

: Level 3

: Level 1

‘NORMAL’

‘ABNORMAL’

(7)

: Level 2

: Level 3

Identifies if it is a Clone (Select only one of these):

Normal

$1 :

Normal

‘1’ :

‘N’

Clone

:

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 11 of 15

'0'

'1'

'N'

'Y'

‘NOCLONE’ :

‘CLONE’ : Clone

(8)

Identifies if the patient is a repetition or not

#00

#01

: Normal patient

: To be repeated

: Normal patient

: To be repeated

: Normal patient

: To be repeated

Note:

It is possible to repeat the patient if:

1. It is not present among the executed patients

2. No free position exists on the plate

3. The patient has already been inserted in the current list

4. The lists of execution are different

5. The Serum/Urine field is different

6. It is also selected as clone

TABEL 2 – INTERNAL VARIABLES

NAK

SYN

ETB

CAN

EM

SUB

ESC

FS

GS

RS

US

SO

SI

DLE

DC1

DC2

DC3

DC4

NUL

SOH

STX

ETX

EOT

ENQ

ACK

BEL

BS

TAB

LF

VF

FF

CR

$21

$22

$23

$24

$25

$26

$27

$28

$29

$30

$31

$14

$15

$16

$17

$18

$19

$20

$06

$07

$08

$09

$10

$11

$12

$13

$00

$01

$02

$03

$04

$05

0x0F

0x10

0x12

0x13

0x14

0x15

0x16

0x17

0x18

0x19

0x1A

0x1B

0x1C

0x1D

0x1E

0x1F

0x20

0x21

0x01

0x02

0x03

0x04

0x05

0x06

0x07

0x08

0x09

0x0A

0x0B

0x0C

0x0D

0x0E

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 12 of 15

SCRIPT EXAMPLES

The examples outlined here are the transformation in script of the standard routine of the patient reception by the analyzer.

Stringn #PatientCode|$15

Char #PatientListType

Char #PatientType

Char #PatientClone

Stringn #PatientCupPosition|$2

Stringn #PatientNumberTest|$2

Set #BeginAnalysesData

Stringn #AnalysesCode|$4

Set #EndAnalysesData

Stringn #CheckSum|$3

The following are the details of the above Scripts:

Stringn #PatientCode|$15

Patient Code of fixed length equal to 15 characters

Char #PatientListType

Type of list (Routine/STAT) as single character

Char #PatientType

Serum type (Serum/Urine) as single character

Char #PatientClone

Identifies if the patient is or is not a clone (single character)

Stringn #PatientCupPosition|$2

Position of serum cup (string of fixed length equal to 2 characters)

Stringn #PatientNumberTest|$2

Number of tests to be executed (string of fixed length equal to 2 characters)

Set #BeginAnalysesData

Beginning of analysis codes

Stringn #AnalysesCode|$4

An analysis code of fixed length equal to 4 characters. It must be entered for each type of test as per qty indicated in the #PatientNumberTest.

Set #EndAnalysesData

End of analysis codes

Stringn #CheckSum|$3

Check-Sum (transferred as a string of fixed length equal to 3 characters)

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 13 of 15

The following examples are the transformation in script of the standard routine for the transmission of a report by the analyzer to the host computer:

Stringn #PatientCode|$15

Char #PatientType

stringn #PatientNumberTest|$3

Set #BeginAnalysesData

Stringn #AnalysesCode|$04

Stringn #AnalysesConcentration2|$7

Set #EndAnalysesData

Stringn #CheckSum|$3

The details of the above scripts are as follows:

Stringn #PatientCode|$15

Patient Code of fixed length equal to 15 characters

Char #PatientType

Serum type (Serum/Urine) as single character

Stringn #PatientNumberTest|$3

Number of results to be sent (a string of fixed length equal to 3 characters)

Set #BeginAnalysesData

Beginning of zone repeated for the number of results to be sent (see

#PatientNumberTest)

Stringn #AnalysesCode|$04

An analysis code of fixed length equal to 4 characters

Stringn #AnalysesConcentration2|$7

Concentration referred to the analysis code as per #AnalysesCode (a string of fixed length equal to 7 characters)

Set #EndAnalysesData

End of zone repeated for the number of results to be sent

Stringn #CheckSum|$3

Check-Sum (transferred as a string of fixed length equal to 3 characters)

4-7. SERIAL COMMUNICATION TEST PROGRAMS

A simple communication program for sending command characters to the analyzer and receive any response.

At the start the only input to the program is the number of the communication port (from 1 to 4).

A blue screen divided into two sections is displayed. In the upper section the characters coming from the analyzer are displayed, while the lower section displays the characters sent to the analyzer.

The only special keys used are F1 to clear the screen and F10 for exiting the program.

The special characters (with values less than 32) are displayed in ASCII notations along with their values.

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 14 of 15

For example the Character EOT - value 4 - will be shown as EOT (4).

To send a special character (with values less than 32 or higher than 124) it is necessary to keep pressed the ALT key and simultaneously write the value of the character to be sent using numerical keys. For example to send EOT it is necessary to keep the ALT key pressed and simultaneously enter the value 4 through the numerical key and then release the ALT key.

A simple communication program that simulates the host computer. At the start it is necessary to identify the number of communication port (from 1 to 4) and the desired procedure (Transmission or Reception).

In case the Transmission is selected the program will ask for patient code (from 1 to 15 characters), the test number (from 1 to 9) and the relevant analysis code for each test (for example: BUN).

It is a good practice to use the same analysis codes, which the analyzer has memorized in the plate actually in use, if otherwise then an error will result in the transmission phase.

Now the program will execute an initialization procedure of communication with the analyzer, will send patient data and wait for the outcome of transmission.

At the end the screen will display the outcome of the operation or show the position number of the plate where the patient has been inserted or explanation of error code sent by the instrument (for example: Patient Code Duplicated).

If the Reception procedure is selected, then the program will begin initialization of communication with analyzer, will ask for data of the next report ready for serial dispatch and show data of relevant downloaded report.

If there are no reports to be received, then a relevant message will be displayed.

Every time the program waits for a response from the analyzer, in case of problem it is possible to abort the current operation by simply pressing the Esc (Escape) key.

Note:

Both the programs must reside in the computer connected serially to the analyzer through appropriate cable indicated in the Operators Manual.

The computer must be an IBM compatible equipped with DOS operative system:

Windows 95, Windows 98, or Windows 2000. The operating systems such as MAC,

UNIX, Windows ME or XP are not supported.

Since the programs operate in DOS ambience, therefore in case the Windows operating system is used then it will be necessary to open a DOS shell (the command Prompts of

MS-DOS is found in the menu Programs, Accessories - accessed through the Start button on the bottom left of the screen).

Both the programs use serial port with the following setups:

Baude-Rate....................... 9600

Stop-Bits........................... 1

Parity................................. None

Hand-shake...................... Hardware

Important Notice:

These two programs are in the installation disk under Utility folder.

Section - IV Software BT3000 PLUS Rev.0, Soft Ver. 8 Page 15 of 15

SECTION - V

REPAIR OR REPLACEMENT OF VARIOUS PARTS

REFRIGERATION

MODULE

COMPUTER

BOX

SERUM

MODULE

REAGENT

MODULE

SAMPLING

ARMS

ISE MODULE

READING

STATION

REAR

PANEL

ACCESS

BT3000 PLUS - EXPLODED VIEW

Figure 5-1

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 sur 22

5-1. ACCESS TO VARIOUS MODULES - INTRODUCTION

The BT3000 PLUS chemistry analyzer requires a series of mechanical adjustments during the production and assembly process to assure optimal performance. The same mechanical adjustment procedures that were followed in the production are outlined here for field maintenance and repair.

The analyzer is based on a modular construction. Each module must be correctly inserted into its proper location. Any positioning adjustments are facilitated by the large tolerances on the mounting holes. It is important to check that the sampling arm movement precisely coincides with all the operating positions. A fine adjustment of positions can be made through the software. During the production phase of the analyzer, a special reference mask (jig) is used to correctly position the various modules in their locations. However, during maintenance and repair, one must take care to operate, step by step, so as not to alter the original assembly. Therefore, it is strongly recommended not to disassemble multiple modules at one time. Instead, the modules should be serviced and calibrated one at a time

5-2. REPAIR OR REPLACEMENT OF THE SERUM/ISE

SAMPLING ARM

Turn off all power to the instrument before replacing or repairing the sample arm.

Remove the casing and all of the parts necessary to access the arm subassembly

(see Figures 5-2 & 5-3). Disconnect the electrical cable and the liquid heater cable. Unscrew the liquid line connector from the appropriate diluter. Unscrew the sampling arm assembly 3 mounting screws. Gently lift the sampling arm module and place it on a safe location. This operation may not seem simple but the figure here facilitates the removal. Replace the repaired or substitute sampling arm module in its appropriate location observing the preceding steps in reverse order.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 sur 22

Arm Head

Needle

Timing Belts

SAMPLING ARM (FRONT)

Figure 5-2

SAMPLING ARM (REAR)

Figure 5-3

Do not tighten the new sampling arm mounting screws to allow for position calibration. Manually swivel the sampling arm to verify coverage from reagent plate to the cuvette plate. Also verify if the sampling needle centers the washing funnel and the reading cuvette correctly by observing the following procedure:

1) Turn off power to the analyzer and unplug the power cord from the wall outlet to avoid electrical shock.

2)

3)

4)

Manually lower the sampling needle all the way into the washing funnel to verify that it centers correctly. Gently lift out needle from washing funnel and move it CCW (counterclockwise) to the cuvette plate.

Align any cuvette on the cuvette-plate with the washing piston, and then carefully slide downwards the washer body until the piston bottoms into the cuvette.

Locate and mark the position of 12th cuvette by counting anticlockwise starting from the cuvette adjacent to the cuvette with washing piston. The latest production instruments have an index mark corresponding to the

12th cuvette position on the rim of the cuvette system housing for facilitating mechanical calibrations.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 sur 22

5) Move CCW (counterclockwise) and lower sampling needle into the 12th cuvette and verify that it centers correctly. If the needle centers properly on the washing funnel and the cuvette then finally tighten the three setscrews on the arm head and the three mounting screws on the arm bracket. If otherwise, repeat preceding steps until the sampling needle is centered correctly, and then securely fasten the screws and continue with the procedure below.

NOTE:

When the sampling arm is substituted or simply removed for service and then remounted, it is highly important to verify the zero position and afterwards check all the other positions observing the procedure as follows: a) Start up the analyzer and let the program bootstrap begin. b) During bootsrap press the keys <Alt>, <Ctrl> and <Shift> simultaneously. c) Let the bootstrap end (a message "ONLY FOR SPECIALIST" appears on the screen). d) Align any cuvette on the cuvette-plate with the washing piston, and then carefully slide downwards the washer body until the piston bottoms into the cuvette. e) Lower the sampling arm needle into the washing funnel and press enter key. Do not touch the sampling arm; leave it in whatever position it is in. f) Enter the mechanical calibration program (icon with gear symbol). g) Carefully perform mechanical calibration of all the positions. Do not overlook any position. h) Perform a general reset (F5). The arm needle should position itself on the center of the washing bowl. Verify the correct position.

In case the sampling needle is not positioned correctly then recalibrate by entering the mechanical calibration program (icon with gears symbol). It is not necessary to turn off the instrument and perform the bootstrap process. Perform a reset by clicking on the icon

Ⓡ

and press the keys ALT, CTRL, and SHIFT simultaneously and then repeat preceding steps d) to h).

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 sur 22

IMPORTANT NOTICE:

Mechanical calibration procedure for the ISE Arm is the same as above except that the ISE Arm needle is first centered on the ISE washing funnel (metallic funnel located on the right of the serum plate) and then moved CCW to any cuvette on the cuvette plate where it centers correctly.

INSTALLATION INSTRUCTIONS

This retrofit kit replaces and upgrades the previous sampling arm head (P/N 07-

04438-01). It simplifies the routine maintenance procedure. The new arm head installation is permanent, and only the tube heater assy must be replaced when necessary.

To install the arm head observe the following step-by-step procedure:

1) Turn off power to the analyzer and unplug the power cord from the wall outlet to avoid electric shock.

2) Gently disconnect the previous arm head tubing and cable connectors.

3) Loosen the 3 setscrews on the rear near the (shaft) and remove the arm head.

4) Unscrew the 2 lateral screws from the new arm head cover and remove cover (Figure 5-6) from the body.

5) Carefully place the new arm head body over vertical shaft (Figure 5-4) ensuring that the locking screw is parallel to the reference slot on top edge of the shaft. Gently push down the arm head body until level with the top edge of shaft and securely tighten the locking screw.

6) Insert the metallic fin into the aperture on the rear of arm head and tighten the screw (Figure 5-5).

Head

Body

Reference Slot

Shaft

Figure 5-4

Fin

Locking

Screw

Figure 5-5

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 sur 22

7) Place the cover on the arm head and gently push down to stop. Ensure that the sample tube is correctly positioned between the metallic fin and the appropriate slot on the rear of cover. Securely fasten the two screws on the sides of the cover (Figure 5-6).

8) Securely connect sample tube and cable to correct connectors.

Cover

Left Sampling

Arm, cut 20 cm from this tube.

Figure 5-6

Sampling Tube with Needle Holder

P/N 08-05029-01

Figure 5-7

NOTE:

The preceding installation procedure is identical for both the sampling and ISE arms (left arm and right arm in BT 3000 PLUS). To facilitate production the length of sample tube (single plastic tube adjacent to the electrical connector - Figure 5-

7) is identical in both the arms. It is necessary to reduce the length of the plastic tube of left arm head (for clinical chemistry sampling) by about 20 centimeters prior to connecting it to the diluter. The length of tube of right arm head (ISE sampling arm) remains unaltered. In the analyzers BT 1000 and BT 2000 PLUS

(without ISE sampling arm) the tube length of sampling arm head must be reduced by 20 centimeters (Figure 5-7) as already outlined

9) It is mandatory to perform calibration of positions with the standard service procedures. This operation is necessary because of possible variations during installation.

10) It is mandatory to perform the calibration of volumes using appropriate procedure. This procedure is necessary because the vertical position of needle may result to be slightly displaced with respect to the previous version of sampling arm.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 sur 22

Final testing:

After terminating the installation procedure, test the system as follows: a) Verify that the new arm head is firmly secured to the shaft (Fig. 5-4, 5-5 and 5-6). Try to rotate the head after holding the shaft stationary. Head should not move, if otherwise then firmly tighten the locking screw. b) Touch with finger the sampling needle, instantly the red LED at the bottom of arm head lights and projects a beam downwards. c) Let few minutes pass and then touch the needle holder, it should feel warm (heated). d) In the program of calibration services repeat the verification of positions and eventually correct them.

Now the new arm head has been correctly installed. Any future maintenance will be much easier as the only part subject to wear and periodic replacement is the

"Sampling Tube with Needle Holder" P/N 08-05029-01 (Figures 5-7 & 5-11), which also incorporates the supply voltage leads for powering the arm head system. The "Sampling Tube with Needle Holder" kit integrates sampling needle, needle holder, sampling tube with heating circuit and appropriate connectors.

Additional Informative Photographs:

Arm Head (Top View)

Figure 5-8

Arm Head (Side View)

Figure 5-10

Arm Head (Bottom View)

Figure 5-9

Sampling Tube with Needle Holder

P/N 08-05029-01

Figure 5-11

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 sur 22

5-2.2. SAMPLING TUBE WITH NEEDLE HOLDER

P/N 08-05029-01 INSTALLATION INSTRUCTIONS

In the arm head the only part subject to wear and periodic replacement is the

"Sampling Tube with Needle Holder"

P/N 08-05029-01 (Figure 5-12), which also incorporates the supply voltage leads for powering the arm head system. The "Sampling Tube with

Needle Holder" kit integrates sampling needle, needle holder, sampling tube with heating circuit and appropriate

Left Sampling

Arm, cut 20 cm from this tube. connectors.

To install the arm Sampling Tube with

Needle Holder observe the following step-by-step procedure:

1) Turn off power to the analyzer and unplug the power cord from the wall outlet to avoid electric shock.

Sampling Tube with Needle Holder

P/N 08-05029-01

Cover

Figure 5-12

2) Loosen (do not remove) the 2 lateral screws from the arm head cover and carefully remove cover by sliding upwards from the arm head (Fig.5-13).

3) Gently disconnect the previous

"Sampling Tube with Needle

Holder" tubing and cable connectors (Figure 5-14). Note that the ISE ARM (on the right side) cable and tubing

Disconnect connectors have different Tube & Cable locations.

4) Loosen (do not remove) the screw (Figure 5-15) on the needle clamp.

5) Carefully disconnect tube (Figure

5-16) from the arm head and remove the defective "Sampling

Tube with Needle Holder"

(Figure 5-17).

Figure 5-13

Figure 5-14

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 sur 22

6) Correctly install the new "Sampling

Tube with Needle Holder" on the arm head and firmly secure the needle holder by tightening the clamp screw (Figure 5-15).

7) Place the cover on the arm head and gently push down to stop.

Ensure that the sample tube is correctly positioned between the metallic fin and the appropriate slot on the rear of cover. Securely fasten the two screws on the sides of the cover (Figure 5-13).

NOTE:

The preceding installation procedure is identical for both the sampling and ISE arms (left arm and right arm in BT 3000

PLUS). To facilitate production the length of sample tube (single plastic tube adjacent to the electrical connector - Figure 5-12) is identical in both the arms. It is necessary to reduce the length of the plastic tube of left arm head (for clinical chemistry sampling) by about 20 centimeters prior to connecting it to the diluter. The length of tube of right arm head (ISE sampling arm) remains unaltered. In the analyzers BT 1000 and BT

2000 PLUS (without ISE sampling arm) the tube length of sampling arm head must be reduced by 20 centimeters (Figure 5-12) as already outlined

Figure 5-15

Figure 5-16

Figure 5-17

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 sur 22

5-3. REPAIR OR REPLACEMENT OF THE REAGENT MODULE

The repair or replacement of the reagent module is relatively complex because of the necessity to remove many parts before the extraction of whole module. It is important to turn off power to the analyzer before any repair or replacement. After the repair or replacement, no mechanical re-calibration is necessary. However, there is the possibility that the sample arm may require an angular position adjustment through software program.

The following situations may require service personnel intervention:

1) Replacement of one or more Peltiers

2) Replacement of the transmission belt

3) Replacement of the drive motor

4) Replacement of the temperature or Hall sensors

For these operations, refer to disassembly procedure and associated photos.

5-3.1. REPLACEMENT OF REAGENT PELTIER MODULES

This operation requires the removal of the front body, computer box, and the front cover. Refer to the photo sequence in the disassembly procedure. Disconnect all of the connecting cables, unscrew the 4 mounting screws securing the Peltier module and remove the module. Turn it upside down and carefully place it on a surface. Unscrew and remove the two heat sinks. Now, observe the exposed group of Peltiers and the electrical connections soldered to a PCB. Check for the defective or interrupted Peltier using the Ohmmeter.

Bear in mind the difficulty of instrumental measurement because of the emission of residual energy produced from the Peltier. The use of a digital tester may provide a faulty measurement. It is recommended to verify the inconsistency of an average measurement rather than the absolute value that is, in theory, approximately 2 Ohm per Peltier.

Generally, a defective Peltier presents a partial interruption with values of some

10 Ohms, which are sufficient to drastically reduce the current flow in the series of

Peltiers, therefore generating a diminished performance of the refrigeration group.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 sur 22

Unsolder the leads of the defective Peltier, unscrew the 2 screws on the metallic disc securing the Peltier element and remove it. Substitute the damaged Peltier with a new Peltier module using thermal grease for dissipation. Make sure to take into account the proper polarity (see Figure 7-1 in Section VII). An incorrect assembly may affect the system performance. Carefully tighten the screws ensuring that these are not over-tightened so as to avoid damage to the ceramic elements of the Peltier. After assembling all of the devices, it is important to recalibrate the temperature control.

REPLACEMENT OF THE REAGENT PLATE

TRANSMISSION BELT

For this operation, it is necessary to remove the case, computer box, and the front cover. Remove the round refrigeration chamber (in aluminum) by unscrewing the

16 mounting screws. Observe the photo sequence for disassembly details.

Loosen the screws of drive motor assembly plate, located on the top left corner, and slide it slightly inward to loosen the belt. Remove the damaged belt and replace it with a new belt.

To reassemble, follow the preceding steps in reverse order. Carefully slide the motor plate outwards to the left to provide the correct tension and tightness to the belt, and then tighten the mounting screws.

5-3.3. REPLACEMENT OF REAGENT PLATE DRIVE MOTOR

The removal operations are similar to the above "Replacement of the Reagent

Plate Transmission Belt". The motor is secured to the plate through 4 screws.

5-3.4. REPLACEMENT OF THE REAGENT TEMPERATURE AND

HALL SENSORS

The removal operations are similar to the "Replacement of the Reagent Plate

Transmission Belt". Two fixing screws on one side secure the rectangular PVC housing of Hall sensor. The sensor requires precise positioning to correctly couple with the magnet on the drive pulley. The Hall sensor assembly must come close to the transmission belt, which interposes between the magnet and the Hall sensor at a maximum distance of 1mm almost grazing the same belt.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 11 sur 22

The temperature sensor is housed in a rectangular metallic container, which is closely secured to the bottom of the circular aluminum reagent chamber with a single screw accessible from the top of the chamber. The Replacement of the temperature sensor does not require any particular care, except that the two parts must be joined perfectly by properly tightening the screw. No temperature verification or calibration is necessary.

5-4. REPAIR/REPLACEMENT - READING STATION MODULE

It is possible to access nearly all the parts of the reading station module for the maintenance and the repair. The analyzer must be turned off prior to repair or

Replacement. Only in the case of the Replacement of Peltiers (located at the bottom of module). It will be necessary to remove this module. The following components are easily accessed without removal of the module:

1) Replacement of the Peltier

2) Replacement of Halogen lamp

3) Replacement of all the tubes of the hydraulic circuit

4) Replacement or maintenance of diluter

5) Replacement of washing piston

6) Replacement and inspection of reading cuvettes

7) Replacement of photometer

5-4.1. REPLACEMENT OF THE CUVETTE PELTIER

For the Replacement of the Peltiers it is necessary to remove the 3 mounting screws (see Figure 3-1 entitled "Top View of the BT3000 PLUS".) To facilitate the removal of a screw located inside, it is necessary to remove the front cabinet. It is important to disconnect all of the cables and tubing before removing the station.

Each Peltier group contains a fan and heat sink and is securely attached with 4 screws to the base of the station. Coat the new Peltier surface with a thin layer of thermal grease and secure it to the base of the reading station with 4 screws.

Tighten the mounting screws until they are snug, ensuring that the pressure is applied uniformly. Do not over-tighten otherwise the Peltier may get damaged.

Ensure leads' polarity is matched correctly. The incorrect assembly of the Peltier can cause serious malfunction of the thermostatic system.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 12 sur 22

3

4

2

1

Replacing the Halogen Lamp

Figure 5-18

5-4.2. REPLACEMENT OF THE HALOGEN LAMP

Precautions for handling the halogen lamp: a) Always allow the burnt out lamp to cool down. b) Never touch the reflector or the lamp with bare fingers. Use a rag when changing. c) If the lamp is touched inadvertently during installation, clean the lamp or reflector with alcohol and dry with a clean, soft cloth before burning. Contamination of the lamp or reflector may reduce lamp performance. d) It is recommended to initially burn the new lamp for about 30 minutes before analyzer operation.

Rear

Cover

LAMP

LAMP REMOVAL (Rear Cover)

Figure 5-19

LAMP

LAMP REMOVAL (Front Shutter)

Figure 5-20

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 13 sur 22

Disconnect the input power before servicing. The replacement of the Halogen lamp can be done by the removal of the rear panel, or by sliding the transparent shutter front panel and then removing the access cover from the deck (Figures 5-

19 & 5-20).

Release the lamp assembly by gently sliding downwards. Loosen the front screws

❷ in the socket ❸ and remove the burnt out lamp ❹.

Insert a new halogen lamp fully into the socket and firmly tighten the screws to secure the lamp. It is recommended to slightly press both of the lamps retaining spring clips ❶ before fitting the lamp. Slide the lamp assembly onto the light cone and orient the lamp socket in the vertical position as shown in the Figure 5-20.

5-4.3. REPLACEMENT OF HYDRAULIC TUBES

There are three tubing and accessory kits for maintenance. A Bimonthly

Maintenance Kit for ISE tubing, Six Month Kit for special tubes exposed to wear and tear, including pump cartridges, etc., and an annual maintenance kit for general tubing for interconnections and liquid transfers.

When installing any tubing kit, it is important that the hydraulic circuit is empty. To avoid any undesired liquid spills, disconnect the external water input before applying any maintenance kit. Turn on the instrument and perform a washing cycle to evacuate all liquid in the hydraulic circuit. When changing tubes, follow the illustrative diagrams (Figure 5-21) on the next page.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 14 sur 22

1

4

(Reading Station)

3

Washer/Diluter - BT 3000 PLUS

2

HYDRAULIC TUBES AND ACCESSORIES

Figure 5-21

5

4

1A

ISE Module

6

BIMONTHLY MAINTENANCE KIT (P/N. 662.1016)

ITEM QTY

1A

5

6

7

2

1

1

1

DESCRIPTION

Diluter/Pinch Valve Tube ISE

Interconnection Tube for GND/Bypass OR CO2 Electrode

ISE Pump Tube

REF - K Tube Manifold

7

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 15 sur 22

SIX MONTH MAINTENANCE KIT (P/N. 11-05219-01)

ITEM QTY

1 2 Diluter/Pinch Valve Tube

1A

DESCRIPTION

2 Diluter/Pinch Valve Tube ISE

2 1 Aspiration Tube for Washing Module

4

5

6

7

2

2 Peristaltic Pump Cartridge

1 Interconnection Tube for GND/Bypass OR CO2 Electrode

1 ISE Pump Tube

1 REF - K Tube Manifold

ANNUAL MAINTENANCE KIT (P/N. 11-05220-01)

ITEM QTY

1

1A

2

2

2

1

Diluter/Pinch Valve Tube

DESCRIPTION

Diluter/Pinch Valve Tube ISE

Aspiration Tube for Washing Module

4

5

6

7

8

2

2 Peristaltic Pump Cartridge

1 Interconnection Tube for GND/Bypass OR CO2 Electrode

1 ISE Pump Tube

1 REF - K Tube Manifold

1 Tubular Filter for Water Container

5-4.4. REPLACEMENT OR MAINTENANCE OF DILUTER

Even though it is unlikely that the diluter will require Replacement, its removal for service is quite simple. Turn off all power to the instrument before servicing. The diluter is secured through 4 screws on its flange and one cable connector for electrical circuitry.

The maintenance involves yearly cleaning of the piston and the transparent

Plexiglas chamber. To perform maintenance disconnect the fluid tubing. Unscrew the 2 screws located on the front of the diluter Plexiglas chamber and carefully remove the Plexiglas chamber exposing the piston. Clean the piston with a soft lint-free cloth. Wash the Plexiglas chamber and the inside of the O-ring with distilled water. After cleaning, gently replace the chamber onto the diluter drive.

Firmly secure with the 2 screws.

The O-ring (P/N 330.5703) must be substituted every 300,000 cycles. Prior to Oring installation, make sure that all surfaces are free of dust, dirt and the like.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 16 sur 22

5-4.5. REPLACEMENT OF THE WASHING PISTON

Turn off the analyzer prior to replacing the washing piston. Push the washing piston carriage fully upward to facilitate the piston removal. Remove all of the silicone tubes. Gently unscrew the piston from the plunger stem by turning clockwise

(Fig.22). In case of difficulty (slippery piston, etc.), use the Piston Grip (Fig. 25) or lint-free cloth to improve the grip. Fit the new piston and fingertighten firmly by turning counterclockwise until it is properly seated against the thread shoulder.

WASHING PISTON

Reposition the carriage into its original position.

Attach all tubing to the appropriate connectors.

5-4.6. REPLACEMENT OF READING CUVETTE

Washer Module

Figure 5-22

It may become necessary to remove the cuvette wheel in order to inspect or replace damaged cuvettes. Generally this operation is necessary when the following malfunctions occur: a) Water is present on the cuvette wheel b) Accidental dropping of foreign matter into the cuvettes c) Accidental damage of one or more cuvettes

Turn off the analyzer. Push the washing piston carriage fully upward to aid in the cuvette wheel removal. Unscrew the 6 screws from the cuvette wheel and carefully lift out. Use a gentle oscillating movement, if necessary, while exercising extreme care to prevent any damage to the cuvettes. Inspect the cuvette wheel, rotor, and housing for the presence of any liquid. Clean and dry if necessary.

Afterwards, diagnose the cause of the problem. Avoid removing the cuvette wheel to wash the cuvettes as this operation will result to be complex because of the use of only distilled water to avoid calcareous build-up. Perform the washing after the cuvette drying or substitution by using appropriate analyzer functions. The foreign objects or physical debris inside the cuvettes can also be removed by turning the cuvette wheel upside-down.

Another solution for cleaning cuvette (analyzer must be turned on) is to use the aspiration tube of the washing piston (on top of the washing plunger) by inserting it all the way into the cuvette for few seconds and then manually rotate the cuvette wheel to move to the next cuvette for cleaning.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 17 sur 22

When replacing the cuvette wheel, position the small guide hole in the cuvette wheel that coincides with the locating pin on the cuvette rotor.

Gently push the cuvette wheel downward onto the rotor. Fasten the cuvette wheel securely by screwing the 6 socket head screws back onto the cuvette rotor. To ensure that pressure is applied uniformly, first tighten all of the screws finger-tight and then tighten them in a crosswise pattern. Do not use excessive force. Gently push downward each cuvette until it bottoms against the depth of the rotor. Attach any disconnected tubing to the appropriate ports. A thorough washing procedure should be performed through the analyzer program before any testing is performed on the analyzer.

NOTE: Another efficient method for cleaning the cuvettes may be used without removing the cuvettes plate from its original position. Disconnect the aspiration tube located on top of the washing piston carriage and insert it into the bottom of the cuvette for few seconds to aspirate any dirt or contaminants. Repeat this procedure after manually rotating the cuvette wheel to clean off the desired cuvettes. The high power suction produced by the vacuum pump will quickly eliminate any dirt or contamination.

CAUTION: It is required that a complete FCC procedure be performed after replacement of one or more cuvettes. If the removal and replacement of cuvette wheel required no replacement of cuvettes, only the "zeroing" procedure is required.

5-4.7. PHOTOMETER REPLACEMENT

The box containing the photometer module is mounted on the underside of the base plate of the light source. Turn off the power to the analyzer before replacing the photometer. The photometer module can be easily removed by unscrewing the 4 mounting screws on the base plate. The new generation photometers (since

July 2004) are equipped with an additional connecting cable (having a pare of male/female connectors) for the Reference Channel (Figure 5-23).

Caution: It is required that a complete FCC procedure be performed after replacement of the photometer.

5-5 INTRODUCTION TO THE COMPUTER BOX

The LCD Display module, with fully integrated touch-screen, is on the front of the computer box. An on/off switch for the analyzer is located on the bottom left of the display. The peripherals, CD-ROM player and the floppy disk drive are accessed through a cover just below the display.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 18 sur 22

All of the connections to the external peripheral devices are located on the rear of the box as follows:

1 USB Port for the Printer

1 Serial-Port for Host Computer

1 Serial-Port for Modem

1 USB Port for UPS

2 PS/2 ports for Keyboard & Mouse

The ASUS main-board with a 2.4 GHz Intel Pentium ® IV processor, 128 Mb RAM,

CD-ROM player, 20 Gb hard disk, floppy disk drive, and the power supply are all located inside the computer box. Product enhancement resulting from new innovations in technology and from our continuing quality improvement effort may necessitate changes in this configuration in the future. However, any eventual changes will be fully compatible with the previous version. A connecting cable composed of lead conductors of the serial port and the 5-Volt supply interconnects the computer box and the process electronics.

5-5.1. REPAIR AND REPLACEMENT OF THE COMPUTER BOX

First, remove the 2 mounting screws on the rear to access the internal parts of the module. Now, it is possible to open the box from right to left so that the rear of the box is exposed to the service engineer. One can observe the DVD player, floppy disk drive, and the hard disk inside the lower opening on the rear of the box.

The following different operations are required for the removal and Replacement of each particular device:

1) Hard Disk: Remove the mounting screws on the supporting bracket and dislodge the hard disk driver.

2) DVD & Floppy Disk: Unscrew the appropriate screws on the underside of the box and remove the DVD or floppy-disk drivers. It is also necessary to remove the plastic front panel of the LCD display module.

3) Main-board and Peripheral Devices: The main-board (motherboard) is secured to an "L" shaped metallic bracket. Remove the 4 mounting screws on the rear and extract the slide. With this operation, it is possible to access the main-board and all the peripheral devices.

4) TFT-LCD Display Panel: Remove the plastic front panel. Unscrew the 4 screws on the corners, securing the display, and remove the LCD display panel.

5) Power Supply: The power supply of the computer is located in the upper section of the box. To remove the power supply, it is necessary to extract the supporting bracket of the main-board and then remove the 4 screws securing the omega shaped metallic bracket.

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 19 sur 22

5-5.2. 8-WIRE ANALOGUE RESISTIVE TOUCH-SCREEN

To remove the touch-screen, it is necessary to use a fine blade for lifting away the glass-backing panel from the display. The touch-screen is secured to the LCD panel with a very fine double-sided adhesive tape.

It is important that the glass-backing panel is precisely centered. Ensure that the active area of the touch-screen matches the active area of the display. When mounting the touch-screen, carefully observe the four angles marked on the perimeter as a point of reference for facilitating the application. The new touchscreen is already equipped with double-sided adhesive tape.

If necessary, replace the touch-screen driver. It is important to install the driver found on the CD provided with the new touch-screen monitor. For application, refer to Section XIV, Paragraph 14-2. Entitled "Computer Module Trouble-

Shooting".

Reading Station - Bottom View

Figure 5-23

Cable of

Reference

Channel

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 20 sur 22

Funnel Cap Opener

(Manutenzione Tappo Imbuto)

P/N 90-05036-01

Serum Plate

(Piatto Sieri)

Washing Piston

(Pistone)

P/N 20-05110-01

Grip Sleeve

(Manicotto)

P/N 662.1025

Cleaning Tool

(Specillo)

P/N 03254

Funnel

(Imbuto)

FUNNEL CAP OPENER

Figure 5-24

Washing Piston Removal & Cleaning

Washing Piston Kit P/N 662.0610

The washing piston (in Teflon) may have occluded holes. Turn off the analyzer prior to changing the piston as follows:

1) Push the washing piston carriage fully upwards with the thumbs

2) Gently unscrew the washing piston from the plunger stem by inserting the supplied "Grip Sleeve" or abrasive paper of fine mesh (to avoid slipping) and using light finger pressure clockwise.

3) Clean small holes on the side of the piston using the supplied "Cleaning Tool".

4) Fit the cleaned piston and finger tighten firmly by turning counterclockwise until properly seated against thread shoulder of the plunger stem. Use "Grip Sleeve" to tighten

Figure 5-25

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 21 sur 22

Section - V Repair or Replacement BT3000 PLUS Rev. 0, Soft Ver. 8 Page 22 sur 22

SECTION - VI

READING STATION

6-1. GENERAL

The Reading Station is a composite system comprehending the following devices:

1) Heated rotor assembly for reading cuvette.

2) 10-channel stationary Photometer module with additional reference channel

3) Halogen lamp with dichroic reflector for photometer

4) Stationary cuvette drum with integrated Peltier-based (heating/cooling element) thermostatic control.

5) Fluidic system consisting of Water (H

2

O) reservoir, diluter, and washer.

6) Electronic circuits located on the rear of Reading Station

The above-mentioned devices are illustrated in the Figure 6-1 below.

Diluter

Pinch-Valve

Cuvette

Rotor

Assembly

Washer

Peristaltic Pump Liquid Sensor

Water Reservoir

Pinch-Valve

Halogen

Lamp

Photometer

Light

Cone

Reference

Channel

Quartz

Prism

Cuvette

Drum

READING STATION

Figure 6-1

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 12

PCB Reading

Plate Interface

Reference

Channel

Peltier Module,

Heatsink and

Ventilator

Drive

Mechanism

READING STATION - BOTTOM VIEW

Figure 6-2

The Biotecnica photometer (Figure 6-1) is a patented product and it consists of a small metallic enclosure containing 10 photometric channels. Each channel has its own interferential filter, photo diode, and amplifier. Each amplifier has a dedicated trimmer for gain adjustment as illustrated in the schematic 15-04649-

01. The channels amplification is factory calibrated to adapt the signal levels to the manufacturing tolerances of various optical components (photodiodes, interference filters etc.). The light beam enters through the unique port in the photometer enclosure, and after passing through a series of beam splitters, it reaches all the ten channels simultaneously. The reason for this approach is the instantaneous processing of numerous signals.

Reference Channel

The reference channel is not inside the photometer box but it is located nearby externally. It is an amplified photodiode embedded in the cuvette drum (see

Figure 6-1 and Figure 6-2) with its cable connected to the photometer for processing by the multiplexer circuit and A/D converter.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 12

The reference channel provides stability to the transmission signal when the photometric system is used in the monochromatic mode. When the photometer is selected in the bichromatic mode e.g. 340/700nm, the reference channel is not utilized as the same function is accomplished by the 700nm channel.

The Light path

The photometer lamp transmits light beam, which converges on the center of reading cuvette, and then through the cuvette it is reflected into the photometer module by the 90º quartz prism located inside the cuvette drum (see Figure 6-1).

A small quantity (approximately 2%) of light flux is captured by the reference photodiode prior to the light passes through the cuvette.

6-2.1. REACTION CUVETTE OPERATING PRINCIPLE

The light is transmitted through the light cone, into the reaction cuvette, and then reflected into the photometer module by the quartz prism located inside the cuvette system housing. The UV specific optical glass transparent cuvettes allow transmission of wavelengths down to 340nm. The function of the photometer module is to monitor and measure the color variations or absorbance values, between different reaction mixtures over a wavelength range from 340nm-700nm.

After a reaction cuvette has passed a photometer measuring position and the reaction mixture has been analyzed, the cuvette moves on towards the washer module where the single piston drains, rinses, and dries the reaction cuvette for re-use.

6-2.2. LIGHT SOURCE

The photometer light source consists of a 12 Volt, 35 Watt tungsten halogen lamp with integral dichroic reflector for the dissipation of infrareds. The lamp is secured through the lamp retaining spring clips on the light cone for easy removal or substitution (see Figure 6-1). This custom made, UV enhanced, halogen lamp provides a high radiation output in the 340nm UV range throughout its operating life of approximately 2000 hours.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 12

The lamp efficiency is controlled by the analyzer, which detects the radiation insufficiency at 405nm when the optical transmission goes below the 50% of original emission level. Bear in mind that the acquisition of 100 % is memorized when the "SETLAMP" procedure is performed after replacing with new lamp.

6-3. READING CUVETTE SYSTEM

The consistent mass of the aluminum cuvette rotor allows for maintenance of temperature uniformity throughout the 34 cuvette wells (Figure 6-3). The stationary cuvette drum in aluminum encompassing the cuvette rotor integrates

Peltier modules, which thermostatically maintain the selected temperature. The

Peltier modules, relative heat sinks, and the cooling fans are affixed on the underside of the cuvette drum as shown in the Figure 6-2.

MAGNET AND

HALL SENSOR

CUVETTES PLATE

CUVETTE

PELTIER

CUVETTE DRUM

ROTOR

HEAT SINK

FAN

STEPPING MOTOR

CUVETTES SYSTEM

Figure 6-3

The selected temperature is maintained to within ± 0.1ºC at the base and ± 0.2ºC in the cuvette wells. The stationary part and the moving part are tightly matched with 0.2mm spacing in-between. However there is a thermal gradient between the two parts, which makes it necessary to perform accurate temperature calibrations.

The regulation of cuvette temperature is highly simplified through the software.

However it requires an appropriate thermometer for calibration adjustments. The calibration procedure is outlined in the Section XIII, paragraphs 13-2. and 13-2.1.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 12

PERISTALTIC PUMP LINEAR ACTUATOR

DILUTER PUMP

DILUTER PINCH

VALVE

READING

STATION PVC

PANEL

WASHER

DILUTER/WASHER MODULE

Figure 6-4

6-4. DILUTER AND WASHER MODULE

LIQ. LEVEL SENSOR

WATER RESERVOIR

WASHER PINCH

VALVE

WASHING PISTON

The upper part of the reading station consists of a PVC panel (Figure 6-4.), which accommodates the following devices:

1) DILUTER: A 470 µl (Full Scale) diluter pump providing the analyzer with high precision, reproducibility, and reliability. The stepper motor and the photo-sensor cable is connected to the Master Power Board (15-04209-

01) on the rear of the PVC panel.

2) PINCH VALVE: A pinch valve, peristaltic pump and sample needle, which complete the sampling system as described in detail in Section VIII entitled, "Sampling Station". The cuvette washer module positioned in a similar manner to the previous version used on BT 2000/3000.

3) WASHER MODULE: The washer module utilizes a bi-directional, linear actuator-based stepper drive mechanism to provide the washing piston carriage rapid linear movement in up/down directions. The piston has a central bore, three equal openings on the external hemisphere, and a tapered body for improved cleaning, emptying, and drying of the cuvettes.

A photo-switch is provided for reset and standby position. The actuator stepper motor and the photo-switch cable connects to the Master Power

Board (15-04209-01) located to the rear of the PVC panel. These are all managed by the microcontroller µC764.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 12

4) TUBING & CONNECTORS: Most of the in/out fluid tubing has "quickconnect" connectors that incorporate shut-off valves to facilitate the removal of the reading station without liquid spills.

5) WATER RESERVOIR: A water reservoir of 55ml capacity has a transparent porthole and an internal liquid level sensor, which emits yellow light when the fluid reaches the maximum level. The membrane pump located on the rear of the reading station PVC panel refills the reservoir. The washing water from the external water supply tank is aspirated through a transparent tube connected to the rear right side of the analyzer.

A filter attached to the transparent tube in the water supply tank and an additional filter on the intake of membrane pump removes undesired corpuscles. In case of liquid level sensor malfunction an emergency overflow tube connected on top of the water reservoir discharges liquid into appropriate container outside the analyzer. The liquid level sensor cable is connected to the Master Power Board (15-04209-01), where the microcontroller (µC 552) monitors the pump through the feedback control with the sensor.

6) PERISTALTIC PUMP: The peristaltic pump is dedicated to supplying the washing water from the water reservoir to the fluidic circuit, which consists of diluter, pinch-valves, sampling tube and the sampling needle. During the washing cycle, the pump dispenses a necessary quantity of water, which flows through diluter, sample tube, sampling needle, and then finally ends up in the funnel for discharging.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 12

6-4.1. DILUTER OPERATING PRINCIPLE

PCB 15-05021-01

Photoswitch

Input/Output

Ports

Figure 6-5

Linear Slide

Piston Linear Stepping Motor

DILUTOR

It consists of a ceramic piston driven by a linear stepping motor inside a transparent thermoplastic chamber. It is high-resolution device, which allows fluid displacement of 1/10th of microliter per step. A driver circuit operating in micro steps actuates the stepping motor. The zeroing of the piston is realized through photoswitch and a miniature metallic shield. The electro-mechanical system moves to and fro on a linear bearing slide.

The diluter module is connected to the control electronics on the "Master Power

Board" P/N 15-04209-01 (located on the rear of the reading station) through a 8pin connector cable.

The diluter aspirates and dispenses fluid when the pinch-valve is in the closed position. The washing of the proper fluidic circuit is performed with the pinch-valve in the open position. In the latter case the peristaltic pump on the right of the diluter is activated to supply the washing water from the water reservoir to wash the diluter chamber, tubing, and the sampling needle. The washing water finally ends up in the funnel located on the analyzer deck for discharging.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 12

LINEAR

ACTUATOR OF

THE WASHER

MASTER LOGIC

BOARD

15-04211-01

MASTER POWER

BOARD

15-04209-01

LINEAR STEPPING

MOTOR OF THE

DILUTER

MEMBRANE PUMP

FOR THE WATER

SUPPLY TO WATER

RESERVOIR

READING STATION - REAR VIEW

Figure 6-6

6-5. ELECTRONICS FOR THE READING STATION SYSTEM

The entire electronics of the reading station, excluding the photometer, is integrated in two boards located on the rear of its PVC panel: the Master Power

Board (15-04209-01) and the Master Logic Board (15-04211-01). See Figure 6-6.

6-6. MASTER LOGIC BOARD (15-04211-01).

This board (Figure 6-6) can be considered the heart of the electronic system. This board accommodates the master microprocessor with a high capacity RAM and

EPROM. The bootstrap of the process program for all of the satellite microcontrollers is executed in this RAM. This software is resident in the hard disk and can be modified if needed. Another µC764 is resident on this board and, together with µP552, processes an analog digital signal. Observing the schematic carefully, the µC552 utilizes the UART for communicating with the IBM through the MAX232 (U7) via the RS232.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 12

The same µP552 utilizes two analog inputs for reading the temperature of the cuvette plate and the vacuum level of the vacuum pump system. The U8A temperature chip and U8B pressure chip amplify the analog signals of the sensors. The µC764 is dedicated to the control of cuvette plate motor and the linear actuator of the washer module. The U2 chip is a Programmable Array Logic

(PAL) device programmed for generating the complex quantity of logic gates used for interfacing the photometer preamplifier with the µP552. The µC764 also uses this gate for connecting to the Master Power Board (15-04209-01) described below.

The U3 chip is an isolated DC/DC converter, which powers the photometer preamplifier. It regulates +5 Volt DC input voltage into a ±12 Volt DC output voltage.

6-7. MASTER POWER BOARD (15-04209-01)

The Main Power Board (Figure 6-6) is the densest board of the analyzer. It accommodates all the devices required to control the stepping motors, valves, etc. for the reading station. However, for practical reasons, another µC764 has been inserted to provide temperature control and regulation for the cuvette plate.

The circuit consists of a comparator connected to the temperature sensor, which is inserted into the bottom of the cuvette housing. The U8 comparator output, along with the U5 chip, regulates the switching which generates a variable voltage in the terminals of the Peltier modules. One section of the comparator controls a relay for inverting the polarity when the ambient temperature requires momentary cooling of the cuvettes system.

The process just outlined produces a relatively coarse temperature regulation of approximately ±1°C. The fine regulation of ±0.1°C is performed by the software implemented in the µP552. When monitoring the temperature of the sensor, the above-mentioned regulator allows a continuous adjustment of the selected temperature.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 12

6-8. INTERFACE BOARD (15-04270-01)

The ISE sampling arm heater, the cuvette motor, Peltier modules of the reading station system and their cooling fans, the Hall sensor, and the temperature sensor are all connected to the Interface Board (Figure 6-2). A bi-color LED indicator is also located on this board to visualize the hot/cold status.

NOTE:

The Master Logic Board, Master Power Board, Photometer, Halogen Photometric Lamp, Diluter

Pump, Peristaltic Pump or any other device on the reading station can be simply accessed through an appropriate cover on the rear panel. This access cover has been designed into the system for facilitating the inspection and maintenance operations. After receiving the information from the central processing unit (IBM), the integrated electronics make the module an independent intelligent peripheral.

6-9. THE 10-CHANNEL PHOTOMETER

The Biotecnica 10-channel photometer (Figure 6-1 and Figure 6-7) is a discrete device and has ten channels with interference filters. Each channel has an independent filter, photodiode, and amplifier. The unique optical system provides for simultaneous illumination of all of the channels. The electronics circuit is capable of communicating and acquiring signals from 2 channels for bi-chromatic reading.

The 10 amplifiers and as many diodes are visible on the schematic (Figure 6-7).

Each amplifier is equipped with a trimmer for gain regulation. The gain adjustment is necessary for adapting the signal level to the manufacturing tolerances of optical components. The two multiplexers, U6 and U7, and their outputs that are connected to the inputs of two A/D converters, U8 and U9, establish the simultaneous selection of two channels. The conversion is serial type. The digital impulses pass through the U10 Gate (74LS132) with Smith trigger function. The two multiplexers are addressed by the µC764 (master logic board) with 4-bit per multiplexer. The electronic circuit of the photometer is powered by ±12 Volts for the analog circuits and +5 Volts for the digital circuits. A 16-conductor flat cable is used for connecting the photometer to the master logic board.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 12

The calibration of each channel is performed in reference conditions (usually at the factory). The following requirements must be observed for the calibration procedures: clean and transparent cuvette filled with distilled water, new halogen lamp, and sufficient warm up time. The diagnostic program can be used for verification and any correction, if necessary. Each channel must generate an output signal between 4 Volts and 10 Volts (theoretical). The voltage values are not visible in the diagnostic program, but the integration values are equivalent if the conversion factors are assumed as reference. In practice, the integration values should be between 14,000 and 28,000 points for all of the channels. This range takes into account the tolerances necessary for different lots of lamps and their related exhaustion. In addition an external photodiode acting as Reference

Channel (Figure 6-2) has a range (unadjustable) of 3000 to 16000 points.

Refer to the diagram of "10-Channel Photodiode Array Photometer" below.

SEMI-REFLECTING

MIRRORS

LIGHT-BEAM

INPUT

REFLECTED

LIGHT

INTERFERENCE

FILTERS

PHOTODIODE

DETECTORS

10-Channel Photodiode Array Photometer

Figure 6-7

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 11 of 12

6-9.1. TECHNICAL SPECIFICATIONS OF THE PHOTOMETER

Optics: Ten (10) static channels with semireflective mirrors (beam splitters), with a light input angle of 90°.

Interference Filters: 340, 380, 405, 436, 478, 510, 546, 578, 630nm at a 8nm hbw and 700nm long wavepass.

Photodetectors: Silicon photodiodes for UV and visible detection 310nm-

700nm. In addition a photodiode for reference channel (Figure

Electronics:

6-2).

Ten (10) multiplexed amplifiers and A/D conversion with 22-bit resolution. The photodiode of reference channel is embedded in the cuvette drum (Figure 6-1 and Figure 6-2.

6-10. FCC PROCEDURE

The FCC (Cuvette Correction Factor) function is essential for correcting the optical path of the cuvette, which in any case does not exceed the tolerance of ±

3%. After introducing the potassium bichromate solution in each cuvette and reading the relevant absorbance, the software creates a correction factor for each cuvette based upon average absorbances. As final result one obtains an equivalent response in absorbance better than 99%.

The FCC procedure is necessary every time one or more cuvettes are substituted.

After the termination of FCC cycle, the analyzer system sets the transmission of all the cuvettes at 100%.

Prior to activating the FCC function, prepare reference solution with 4.3 grams of potassium bichromate powder in 1000 ml of H

2

SO

4

0.1 N. Afterwards dilute this solution 1/100 with H

2

SO

4

0.1 N. The absorbance measurement for this product is

400 units at 340nm. The interference filters 340/700nm are used for the test.

During the FCC process the system performs: washing, zeroing, introduction of bichromate solution, absorbance readings, and the creation of correction factors.

Once the FCC is completed, the system presents simultaneously the absorbance values of all the cuvettes before correction and the percentages of corrections.

The program generates a FCC error if one or more cuvettes require a correction of more than 3%. In this case, it is recommended to repeat the FCC procedure to exclude any possibility of other causes. If necessary, substitute the cuvette.

Section - VI Reading Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 12 of 12

SECTION VII

REAGENT SYSTEM

7-1. GENERAL

The reagent module system incorporates an appropriately insulated reagent chamber (in aluminum) for reagents refrigeration. The reagent chamber accommodates the rotating reagent plate (divided into sectors) where trays with wedge-shaped reagent bottles are inserted. Each reagent tray (removable) can accommodate 5 large wedge-shaped reagent bottles (50ml or 80ml) snap-fitted with 5 smaller reagent bottles (10ml or 20ml), for a total of 80 reagent bottles.

Two large heatsinks with eight (8) Peltier assemblies are affixed to the underside of the reagent module. A cross-flow blower located near the heatsinks, removes the air towards outside. An electronic circuit in feedback with temperature sensor regulates the electronic current of Peltiers and ventilator, thus realizing the temperature monitoring system. A stepping motor driven belt-drive/pulley mechanism provides rotary motion to the reagent plate.

The temperature uniformity throughout the reagent chamber is maintained at approximately 12ºC. The temperature inside the reagent chamber is maintained constant up to 26ºC of ambient temperature. Any increase beyond this ambient temperature value will consequently increase the reagent chamber temperature considerably.

7-2. REAGENT PLATE CONTROLLER - 15-05211-01

(Valid from July 2004 onwards)

The board 15-05211-01 is mainly dedicated to the barcode scanner, stepping motor, temperature monitoring, and the reset sensor for the reagent plate.

It consists primarily of a µC U3 87LPC767, which controls the actuation of stepper motor connected to the connector J3.

The stepping motor provides rotary motion to the reagent plate accommodating reagent trays with reagent bottles. The isolation between the driver A1 (power constituent) and the µC U3 (digital element) is obtained through the optoisolators U4 and U5 (TLP2630).

The µC U3 (with gates P1.0 and P1.1) and the integrated circuit U6 control the signals from the barcode module connected to J5.

The Hall sensor is connected to the board through J4. The reagent plate resetting occurs when the Hall sensor is face to face with its magnet located in the reagent pulley.

The refrigerator temperature control is accomplished through the comparator U7A

(MAX492). The voltage present at pin 1 of the comparator reaches the gate P0.4 of U3 and its value corresponding to the reagent plate temperature determines the amount of voltage to be supplied to the Peltier and as well as turning on or off the cross blower fan.

The U7B part of the comparator is dedicated to the control of the safety interlock sensor connected to J6. This sensor stops the plate movement when the reagent access cover is opened.

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 6

The gate P0.7 of µC U3 sends the command bit of optoisolator U8 (TLP521-1). A low logic level of P0.7 determines the simultaneous opening of the MOSFETs Q1 and Q2

(BUZ111S) and the consequent inhibition of the peltier modules of the refrigeration system.

The gate P1.6 of U3 controls the high velocity optoisolator U9 (6N136). This optoisolator drives the digital potentiometers PD1 and PD2. The potentiometers through their ohmic variation determine the Peltier supply voltage value. An approximate voltage of 26VDC corresponds to the higher cooling capacity and the approximate voltage of 16VDC corresponds to the lower cooling capacity. The power supply is interfaced through the connector J2. The NTC1 sensor protects the reagent controller module against overheating. The high temperature inside the module (>70ºC) produces a voltage variation on the gate P0.6 of U3. The intervention threshold is fixed at 4.5V. During the alarm condition the Peltier modules are disabled and an alarm message is displayed on the analyzer screen.

The gate P0.0 of U3 drives the optoisolator U11 (TLP521-1). The low level logic of P0.0 determines the opening of the transistor Q3 and the power off to the cross blower ventilator. The operating logic turns the cross blower fan ON or OFF when the reagent plate reaches the preset temperature (±0.5º) in the analyzer setup to avoid ice formation in the reagent plate with ambient temperature up to +12ºC (values beyond the operating range).

The glowing LED (DL5) indicates the activation of cross blower fan.

This board, compared to the previous board, has the advantage of functioning also when the analyzer is in the standby mode, without any supply voltage from the IBM.

The supply voltage for the µC 767 is realized on board through a DC/DC converter, which is powered by the same source that energizes Peltiers. The temperature sensor is connected to the gate A/D of the 764 for reading the value in ºC. The temperature monitoring system is controlled digitally by regulating nominal 24V in the range of 16V to

28 Volts.

Below a certain temperature (approximately 5ºC), it is not possible to further reduce the current of the Peltiers. Therefore to control the temperature, the operation of cross-blower fan is interrupted for the time necessary to reset the selected temperature. The temperature is selected in the instrument setup and the temperature can be set between

5ºC and 15ºC. The set temperature is maintained reagent chamber base to a tolerance of

+/-2°C as long as the ambient temperature does not exceed the 26ºC. Beyond 26ºC it is possible to have a considerable increase in the set temperature. If the µC reads a temperature higher than approximately 40ºC, then it interrupts the current to the regulation circuit assuming that there is a problem in the system. For example a defective or damaged fan or an exceptionally high ambient temperature (40ºC) may generate an excessive temperature in the reagent chamber. One can observe in the electrical schematic the NTC near the power connectors. It has the function of heat sensor, which may be produced by false contacts in the connections or some other problems in the printed circuit board. In this case the MOSFET Qx interrupts the Peltier current, when the temperature in the interested zone is approximately 70ºC.

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 6

7-3. REAGENT PLATE CONTROLLER - 15-04313-03

(OBSOLETE - used on Analyzers up to June 2004)

This module designed for reagent plate management is housed in an easily removable metallic container. The electronic devices on this board have the following functions: a) Reagent plate drive motor control. b) Zero position sensor control. c) Reagent plate temperature control. d) Barcode reading and interface.

This module is located on the rear of the reagent chamber and is easily accessible for service or repair through the small panel on the rear of the analyzer.

The Reagents Plate Controller Module (15-04313-03) is controlled by µC87LPC764. This microcontroller manages the stepper motor through two opto-couplers, U3 & U4, which isolates the power driver A1 from the digital circuit.

The µC764 receives signals from the barcode module through the U5 chip (Max 232). The reagents chamber temperature is controlled through a U6A comparator and the Q1 transistor. The control temperature is fixed at about 5°C by using the remote control of the power supply (Lambda

Alpha 400W). The J2 connector connects the power supply to the controller circuit of the reagent plate. The Q2 transistor functions as a switch to interrupt power supply to the Peltiers through the

µC764 (pin 14) whenever requested by the software. The circuit for the control of the Peltier voltages has an additional function of interrupting power to the refrigeration system in case the temperature inside the reagents chamber exceeds 40°C. The circuit, which generates this function, consists of a U6B comparator and an U7 opto-coupler. The Hall sensor (zero position), temperature sensor, and the Peltier modules are all located under the reagent chamber.

7-4. REAGENT REFRIGERATION SYSTEM

The reagents refrigeration system comprises reagents chamber, refrigeration module with the Peltier modules, conductive spacers, and heat sink, along with the reagent pulley drive assembly.

Peltier Modules

Refrigerator

Interface Board

Terminals

Soldering

Details

Insulation

Block

Heat Sink

Reagent Refrigeration Module

Figure 7-1

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 6

7-5. REAGENT & SERUM BARCODES

The available codes for sampling procedure are in the memory in blocks of 5 codes. To insert a new block (5 codes) it is important to import data using a floppy disk. The following blocks are available at the present:

CODE39, CODE39, FULL ASCHII, CODABAR, CODE128

EAN128, CODE93, PLESSEY, PHARMACODE, EAN13

UPCA, UPCE, EAN13 AddON2, EAN8 AddOn2, UPCA AddOn2

UPCE AddOn2, EAN13 AddOn5, EAN8 AddOn5, UPCA AddOn5, UPCE AddOn5

Reagents Barcodes

For the reagent system the code used is type EAN13.

1°, 2°, 3°:

4°:

5°:

6°, 7°, 8°:

Numeric analysis code, for example: 128 (GLU)

Type of tube (for example: 80ml)

Reagent 1° or Reagent 2°

Expiry date, year, and month (for example: August 2004 =

408)

9°, 10°, 11°, 12°: Progressive number of 4 digits from 0 to 9999

The algorithm, which manages the control of utilization of the tube, is as follows:

A password establishes if one desires to operate the analyzer as a system:

"OPEN" or "CLOSED"

In the last case (CLOSED), when the tube is empty it cannot be reused if refilled with another reagent. There are other control parameters to avoid the reuse of the same sample tube, as for example the number of possible tests, the decrement of the consumed liquid, the expiry date etc.

Obviously it is possible to use the instrument by reading only the analysis code for positive detection through use of another password.

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 6

Reagents Tray

Timing

Belt

Barcode

Module

Drive

Motor

Peltier

Module

Heat Sink

Conductive

Spacer

Refrigerator

Interface

Board

REAGENT

MOTOR

Detent Mechanism

Reagent Chamber

Motor Plate

Reagent Pulley

Temp. Sensor

Hall Sensor

REAGENT REFRIGERATION SYSTEM - EXPLODED VIEW

Figure 7-2

REAGENTS DRIVE MOTOR

Figure 7-3

Cross-Flow

Blower

REAGENT MOTOR

CONNECTOR

WHITE-BLU

BLUE

WHITE-RED

RED

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 6

Reagent

Hall Sensor

Reagent

Barcode

Reagent Temperature

Peltiers,

Air Blower

I 2 CBUS

Power

Supply

Reagent

Motor

REAGENT CONTROLLER MODULE

Figure 7-4

AUX

Section - VII Reagent Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 6

SECTION - VIII

SAMPLING STATION

8-1. GENERAL

The removable sample plate is protected by a transparent dust cover with various apertures for the sampling needle entry during operation. The sample plate can be placed in any position because the home position will automatically be recognized once the reset has been activated.

The primary tubes can be inserted without removing the plate. Place the tube in the position indicated by the flashing red LED. An optical sensor will differentiate the primary tubes from the cups used for the samples. A barcode reader automatically identifies the position and the data concerning the sample.

The two sampling arms are located on the right side of the analyzer deck. On each sampling arm the stepper motor belt driven mechanism causes the needle to move in up and down linear motion. Another stepper motor drive a belt driven pulley mechanism which swivels the sampling arm to the right or left positions across the deck to position the sampling needle for the aspiration and dispensing procedures.

The left sampling arm can position the sampling needle for aspiration, dispensing, and mixing on the reagent chamber, serum plate, reaction cuvettes, the wash funnel, and the "home" position. The right sampling arm can position the sampling needle on the sample tubes of serum plate, ISE reagent bottles, ISE funnel, and the wash funnel and the "home". It also positions the sample needle over reaction cuvettes for aspiration, dispensing, or mixing.

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 10

Both arms have a built-in facility for the pre-heating reagents through special heating tube. They also have the capability to measurement of the remaining reagent volume in the bottles through a built-in liquid level sensor through an Arm

Liquid Sensor Emitter (15-05023-01). All other devices, including, the diluter, peristaltic pumps, pinch valves, serum plate drive motor, sample arm drive motors, liquid temperature control circuits, and the electronics, are housed inside the analyzer.

The stepper motor for the angular motion of the arm, can generate high frequency oscillations for mixing. The rapid angular movements providing efficient high frequency oscillations of the sampling needle, which when immersed in the cuvette can mix the dispensed fluids. The entire module is an intelligent peripheral slave, which autonomously performs the sampling procedures after receiving information from the Master Logic Board.

Sensor LED

SAMPLING ARM WITH HEATER

Figure 8-1

Sample Needle

8-2. SAMPLING ARM MODULE

The sampling arm module is an electro-mechanical system for provides the sampling needle with vertical (up/down) and angular (swiveling) motion. The Arm

Controller Board (15-04765-01) containing a micro-controller for actuating the stepper motor and is located on the lower section of this module, near the vertical drive mechanism. The liquid sensor circuit is located inside the needle head.

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 10

8-2.1. ARM CONTROLLER BOARD (15-04765-01)

The arm controller board is the basic circuit for controlling arm movement. The

µC764 controls the power to the A & B dual-driver for the sampling arm up/down

& swivel motions through the opto-coupled gates. The "home" or zero position of the sampling arm is realized through a photo-switch and shutter on the base of the motorized shaft mechanism. An additional sensor module is located at the upper section of the drive mechanism.

Arm Head

Needle

Timing Belts

Sampling Arm (Left) ISE Arm (Right)

SAMPLING ARM MODULE

LED

Figure 8-2

SAMPLING ARM (FRONT)

Figure 8-3

Setscrews

Controller

Board

15-04765-01/02

SAMPLING ARM (REAR)

Figure 8-4

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 10

8-2.2. ARM LIQUID SENSOR & EMITTER BOARD (15-05023-01)

It is a microcontroller 764 based small electronic circuit board located inside the arm head. The 8Mhz oscillator of µC764 is used for realizing amplitude modulation of signal transmitted from needle.

The variation of amplitude generated by the needle contacting liquid is controlled and converted by the µC764 in a chain of 40Khz impulses through infrared LED and simultaneously the red Led indicator at the underside of the arm head provides visual repetition of the infrared LED. The circuit is powered by the 5 Volt generated by a low-dropout linear regulator, which obtains the voltage from the downstream of supply line coming from the reading station. No calibration is required for this circuit.

8-2.3. ISE SAMPLING ARM MODULE

The ISE sampling arm module is identical to the preceding sampling arm description. It consists of a drive mechanism, stepper motors for the angular and up/down motions, the Arm Liquid Sensor & Emitter Board (15-05023-01), ISE electronics, and arm controller board. Refer to Figure 8-2 entitled "Sampling Arm

Module", for the technical information.

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 10

8-3. THE SERUM MODULE

The serum module is made up of the stepper motor drive mechanisms for serum plate movement, electronics, primary tube sensor with electronics, ISE sensor and electronics, and the Serum Plate Controller Board (15-04422-01).

SERUM

PULLEY

PRIMARY TUBE

SENSOR (LEFT)

WASHING

FUNNEL

SERUM

CHAMBER

BASE

PLATE

WASHING

FUNNEL

DRIVE

BELT

HALL

SENSOR SERUM

MOTOR

BARCODE

MODULE

PRIMARY TUBE

SENSOR (RIGHT)

SERUM

CHAMBER

SERUM MODULE - TOP VIEW

BASE

PLATE

BALL - BEARING

HEAD

SERUM

PULLEY

SERUM

MOTOR

SERUM MODULE - FRONT VIEW

SERUM MODULE

Figure 8-5

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 10

The new design of the sampling module integrates the entire sampling system into a single module with the following features: a) Autonomous testing of the module before incorporating into the analyzer b) Compact hydraulic circuit providing stabilized flow efficiency c) Hydraulic circuit hidden from view for safety d) Technical assistance facilitated by the ability to substitute components with other tested sampling modules

8-3.1. SERUM PLATE CONTROLLERS

The serum plate controllers are a group of various electronic circuits applied to the removable sampling module. The main controller circuit is housed in a metallic container located near the serum chamber. The additional circuits are firmly secured to the rim of the serum chamber. These electronic circuits perform the following functions: a) Control of the serum plate stepper motor b) Acquisition and management of the barcode data of the serum plate c) Control of the Hall sensor for "home" or zero position d) Control of sensors detecting primary tube sensor presence

8-3.2. SERUM PLATE CONTROLLER BOARD (15-04422-01)

This board housed in a metallic container accommodates the µC764, which controls the serum stepper motor through the U4 and U5 opto-couplers, and the

A1 driver. The barcode data is acquired and processed through the U3 MAX232 chip. The module receives the data from the Hall sensor at the zero position, connected to J2, as well as the signals from primary tube sensors incorporated in their independent circuits located on the left and right of the serum chamber.

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 10

8-3.3. LEFT SERUM PRIMARY TUBE SENSOR

BOARD (15-04280-01)

This supplementary circuit is located on the left of the serum chamber. It accommodates a noninvasive infrared detector housed in a PVC body, for the detection of the primary tube. This circuit board is connected to the Serum Plate

Controller Board (15-04422-01) through the J1 connector, passing through the another supplementary circuit board on the right. Close by, there is a Hall sensor module connected to the Serum Hall Sensor Board (15-04282-01) on the serum plate, which is connected by the J2 connector, to the Serum Primary Tube Sensor

Board (15-04280-01).

The Hall sensor uses the Smith trigger, while the primary tube sensor uses the

U2A-B amplifier in the A.C mode. The emitter LED EM1 is impulse-driven through the U1C oscillator.

8-3.4. RIGHT SERUM PRIMARY TUBE SENSOR

BOARD (15-04278-01)

This supplementary circuit is located on the right of the serum chamber. This circuit is identical to the one outlined in the preceding paragraph, but without the control for the Hall sensor. For operating characteristics, refer to the preceding paragraph. The J2 connector attaches to the identical board on the left. The J1 connector interconnects these two identical boards through a flat cable to the

Serum Plate Controller Board (15-04422-01).

BOARD 15-04422-01

I 2 C BUS

SERUM BAR-CODE

I 2 CBUS

SERUM MOTOR

VACUTAINER SERUM "R"

SERUM PLATE CONTROLLER MODULE

Figure 8-6

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 10

This is a high quality laser scanning & CCD module (Figures 8-5, & 8-7). It is connected to the "Serum Plate Controller Module" through the serial port. It can automatically read 6 different types of codes. It is also possible to reprogram the scanning modules with additional codes during installation. Bt 3000 PLUS has barcode scanning modules for the reagent module and the serum module.

Important Notice:

Any modification to the Variable Serial Protocol (refer to paragraph 4-6. "Variable

Serial Protocol" in Section IV) is restricted to qualified personnel only. The

Biotecnica Instruments S.p.A. guaranties the correct performance of the internal serial protocol. The responsibility for any malfunction arising out of any modifications to the scripts of the Variable Serial Protocol rests with the customer.

WARNING

This information regards the setting up of the barcode for sample tubes identification. The reading of the sample barcode label has the same progression as patient code.

For example: Once a patient code of 15 characters has been entered, then a code of 8 characters followed by 7 empty spaces to reach the 15 characters is sent.

The code read on the barcode label must have the same sequence 8 + 7 for correct detection.

8-5. SERUM & REAGENT BARCODES

The available codes for sampling procedure are in the memory in blocks of 5 codes. To insert a new block (5 codes) it is important to import data using a floppy disk. The following blocks are available at the present:

CODE39, CODE39, FULL ASCHII, CODABAR, CODE128

EAN128, CODE93, PLESSEY, PHARMACODE, EAN13

UPCA, UPCE, EAN13 AddON2, EAN8 AddOn2, UPCA AddOn2

UPCE AddOn2, EAN13 AddOn5, EAN8 AddOn5, UPCA AddOn5, UPCE AddOn5

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 10

Reagents Barcodes

For the reagent system the code used is type EAN13.

1°, 2°, 3°:

4°:

5°:

6°, 7°, 8°:

9°, 10°, 11°, 12°:

Numeric analysis code, for example: 128 (GLU)

Type of tube (for example: 80ml)

Reagent 1° or Reagent 2°

Expiry date, year, and month (for example: August 2004 = 408)

Progressive number of 4 digits from 0 to 9999

The algorithm, which manages the control of utilization of the tube is as follows:

A password establishes if one desires to operate the analyzer as a system:

"OPEN" or "CLOSED"

In the last case (CLOSED), when the tube is empty it cannot be reused if refilled with an another reagent. There are other control parameters to avoid the reuse of the same sample tube, as for example the number of possible tests, the decrement of the consumed liquid, the expiry date etc.

Obviously it is possible to use the instrument by reading only the analysis code for positive detection through use of another password.

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 10

SERUM PLATE

MAGNET AND HALL

SENSOR

WASHING FUNNEL

PRIMARY TUBE

SENSOR LEFT

HALL EFFECT

SENSOR

SERUM

PULLEY

BARCODE

MODULE

SERUM MODULE - EXPLODED VIEW

Figure 8-7

WASHING FUNNEL

PRIMARY TUBE

SENSOR RIGHT

SERUM

CHAMBER

SERUM PLATE

CONTROLLER

MODULE

SERUM

MOTOR

Section - VIII Sampling Station BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 10

SECTION - IX

ION SELECTIVE ELECTRODES (ISE) MODULE

9-1. GENERAL

The ISE module, located on the right side of the analyzer, is used for the determination of electrolytes, in human serum and urine. The analysis method is based on the Ion Selective Electrodes (ISE) technology as the electrodes respond to the ions of the molecules respectively in conformance with the following equation of NERNST:

E = E0 + RT/Nf log aM+

The BT3000 PLUS processes the electrolytes independently from the clinical chemistry. In fact, the module has dedicated devices like a sampling arm, diluter, and a hydraulic circuit. The specific operation method is outlined below.

A sample of serum or urine is aspirated and diluted with buffer solution in the ratio of 1/14 and then dispensed in the mixing funnel. A peristaltic pump, positioned downstream of the electrodes, aspirates the solution until the bubble sensor detects an air segment, which indicates the final part of the solution. The diluted sample remains in the electrodes for a short period of incubation and is read for four seconds. Afterwards the reference solution is dispensed into the mixing bowl and follows the preceding routine. The values of baseline potentials are subtracted from the sample values for zeroing any eventual drift. The system is calibrated using two standards of known values to obtain a slope. The reference

(REF) and buffer solutions (BUF) are concentrated and are diluted automatically with distilled water in a 1/10 ratio during the sampling phase.

A second arm identical to that utilized on the Sampling Module, is used for the sampling of the electrolytes (refer to paragraph 2-4.2.). The elaboration of the data can also be performed in an independent way. The ISE Sampling Arm is located to the right of the Serum Module so that the aspiration needle can easily draw the Sera, the Standards & the Controls in the serum plate. The needle also aspirates the cleaning solution, the Pepsin, Buffer, and the Baseline from the appropriate bottles and funnels of the ISE Module.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 10

Mixing

Funnel

Potassium

Electrode

Sodium

Electrode

Chloride

Electrode

Vertical

Track Rail

Bypass or CO

2

Electrode

ISE MODULE ARRANGEMENT - FRONT VIEW

Figure 9-1.

Bubble

Sensor

Tube

Guide

Controller

Board

Pinch Valve

Preamplifier

Reference

Electrode

Ground

Electrode

Ise Interface

Board

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 10

Electrodes

Cover

Diluitor

Mixing Funnel

Bubble Sensor

ISE Pinch

Valve

ISE Module - View "A"

Figure 9-2

Pinch Valve

Dilutor

Peristaltic

Pump

ISE Waste

REAGENTS HOLDER

Saline Solution Container

Buffer Container

Baseline Container

Pepsin Container

Washing Solution Container

ISE Module - View "B"

Figure 9-3

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 10

Peristaltic

Pump for

Washing

Electrodes: BY-PASS or CO

2

(Optional)

FRONT VIEW

ISE Module

Electrodes location

Figure 9-4

GROUND Electrode

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 10

The ISE module accommodates the ISE electrodes, electronics, hydraulic circuit,

ISE mixing funnel, bubble sensor, and the pinch valve. The highly integrated single module solution provides a very compact physical design. The remaining hydraulic circuit is located nearby on the right side and includes two pinch valves, diluter pump, and two peristaltic pumps driven by a DC motor and a stepping motor.

During instrument operations, the ISE module is out of the view under the cover of the analyzer. However, it can be raised upward and removed by disconnecting the peristaltic pump tube, for inspection or maintenance by the operator or service personnel. This module can be easily be accessed by lifting the little cover on the right side of the analyzer. The location of the ISE funnel directly over the electrodes minimizes carryover and residual bubble interference. The ISE controller board is an intelligent peripheral device. It receives information from the master processor, and using the program resident in its slave processor all the operations relevant to the ISE module are performed.

9-2. SUBASSEMBLIES OF THE ISE MODULE

For the following subassemblies, refer to Figure 9-1, 9-2, 9-3 & 9-4 on the preceding pages.

1) ISE controller board 15-04320-01

2) ISE preamplifier board 15-04440-01

3) ISE interface board

4) Diluter pump module

15--04503-01

5) Peristaltic water pump

6) Waste peristaltic pump

7) ISE pinch valve

8) Diluter pinch valve

10) ISE mixing funnel

11) Saline solution container (BSS)

12) Buffer solution container (BUF)

13) Reference solution container (RES)

14) Enzyme (Pepsin) solution container (ENZ)

15) Washing Solution container (WAS)

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 10

The ISE controller module is a group of electronic devices for the operation of ISE module. The controller module consists of a metallic container divided in two parts

(fixed supporting bracket integral with analyzer base having two rail tracks and the mobile part with electrodes and electronics). The mobile part is removable for routine maintenance and servicing by technical personnel.

The electronic circuit of the ISE module consists mainly of a high impedance preamplifier for the electrodes, an analog-to-digital converter, and a µC767 for data processing. A µC764 is also mounted on the same circuit and is dedicated to the control of diluter pump, photo-switch, and the diluter pinch valve. The separate control of the diluter pump group allows the same diluter pump to be used as required for serum sampling and diluting.

9-2.1. ISE PREAMPLIFIER BOARD (15-04440-01)

This ISE preamplifier board is inside a module affixed to the right side of the electrodes and is connected to the ISE Controller Board through a connector.

Three dual-chips (two chips in a single housing) are mounted on this board. Each chip of the dual-chip is an electrometer and is connected to the appropriate electrode through a unity-gain as shown on the schematic. On the same schematic, besides the terminals for the ISE electrodes, there is a terminal for connecting the ground electrode at reference voltage of 2.5 Volts called the virtual ground. The preamplifier is powered by a DC/DC ±5 Volt mini-converter located on the controller board.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 10

9-2.2. ISE CONTROLLER BOARD (15-04320-01)

The controller board is connected to the ISE Interface Board (15-04503-01) at the bottom through an Euro Connector. When the ISE module is raised for maintenance or service, it is disconnected electrically from the ISE Interface

Board. However, it is possible to use an extension cable to activate the module for any functional test that is required.

A 7714 chip Analog/Digital converter is located on the controller board. This is a special A/D converter with filter and amplification functions that are programmable through the micro-controller. This chip filters and amplifies the analog signals after acquiring them from the preamplifier. It has 24-bit of resolution and can convert weak signals into highly stabile and precision signals. The A/D converter is controlled by µC767 (U11).

The µC767 (U11), utilizing opto-electronic gates (section B of the Motors Driver

15-04408-01), drives the stepper motor of the ISE peristaltic pump. Other devices controlled by the micro-controller are the J4 bubble sensor, the U12 temperature sensor, and the ISE pinch valve. The temperature sensor is used for monitoring the ambient temperature near the electrodes. If there is an excessive variation of temperature during the test run, a prompt is generated to repeat calibration.

The second µC764 (U2) controls as follows: the diluter system using the section A of the driver A1, the reset photo-switch, and the pinch valve of the diluter. The opto-isolated gates that separate the digital section from the power section are also visible on the schematic.

The following external devices are connected to the ISE module through the ISE interface board located underneath the module: diluter pump, peristaltic pump, and the pinch valve.

The bubble sensor uses an opto-isolated device as a sensing element. The translucent plastic tube from the ISE mixing funnel passes through the appropriate channel in the bubble-sensor. The variations of signals produced by presence or absence of liquid is detected by the µC 767, which through an appropriate software algorithm signals the presence of bubble.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 10

9-3. COMMONLY ASKED ISE QUESTIONS

The question and answer list below can assist regarding routine system operation, maintenance and the appropriate answers with relevant technical procedures.

Exercise extreme care to ensure that the system is regularly provided with properly maintained and care should be taken to avoid any problems and malfunctions.

COMMONLY ASKED ISE QUESTIONS

Questions Answers

Should the samples be No. The analyzer performs the dilution manually diluted? automatically.

Can I test urine samples automatically and together with serum?

Yes. It is not necessary to pre dilute the urine sample, use the same calibration procedure as for serum.

Which parameters should be set on the analyzer?

Initially set the date, hour, and the desired measurement units. Only the concentration values of standards when the lot has changed. analyzer everyday? hours and the analyzer will prompt the to recalibrate as required.

Can the analyzer operate unattended?

Yes. The analyzer can operate incessantly without problems.

Do the reagents deteriorate easily?

No. Care should only be given to the Buffer when using the CO

2

electrode. It is recommended to close the buffer container at the end of use to prolong the life. Overexposure to air by the Buffer will diminish the CO

2

slope.

Can I substitute similar No. The ISE reagents are very specific and reagents? substituting may affect the precision of the analyzer, as well as the integrity of electrodes.

How do I maintain the Close them immediately after the use. Do not standards? contaminate them! The accuracy of the samples is dependent upon the quality of the standards. Do not expose them to the sources of strong light and the heat.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 10

COMMONLY ASKED ISE QUESTIONS (contd.)

Can the analyzer suddenly not produce an ISE result?

Yes, when the reagents are finished.

What has happened if all test values are high?

Samples may have exposed to air for more than half an hour on the sample plate or a calibration was performed with a contaminated standard.

Why is the Cl and CO

2 electrode slopes represented in negative numbers?

The potentials of the Cl and CO

2

electrodes are inversely proportional to the concentration.

What happened if the Na & K slopes are negative and the

Cl & CO2 slopes are positive?

The two standards were placed in the inverted positions on the sample (serum) plate.

Is it normal for the analyzer to alert the user with messages like: Reagent Empty, Blocked

Pump, Air in Sample, etc.?

Occasionally It is acceptable. If the system detects a transiting air bubble in the hydraulic circuit. Certainly a momentary stop is preferable than an erroneous result. It is important to perform a prime after replacing exhausted reagent so that the hydraulic circuit is completely filled up with fluid. This reduces the possibility of a bubble formation to minimum.

Can the hydraulic circuit If maintained properly, the hydraulic system will occlude completely? flow continuously. If an obstruction should occur, clean the chloride electrode, with the appropriate tool, first before attempting to clean any other electrode. In case the problem persists then replace all the fluidic tubes with a new tubing kit.

Can the Ground Electrode Yes. Even a partial occlusion in the ground influence the quality of results? electrode hydraulics may affect all the channels.

Which actions are necessary prior to turning off the it is essential to disconnect all the tubes and leave analyzer?

If the analyzer is not to be used for a long period, the electrodes disengaged in their lodgings. The tubes of peristaltic pumps should be released from their supporting brackets and manually manipulated externally to restore roundness and to avoid sticking. When the analyzer is going to be

What are the consequences of incorrect maintenance? used again then reconnect all the disconnected tubes and recalibrate the analyzer at least twice.

Use new Buffer solution.

Apart from generating erroneous results, it may cause irreparable damage to the electrodes. Use of incorrect fluids for cleaning the system may also cause serious damage to the analyzer. For general cleaning in extreme circumstances of the

ISE funnel, electrodes, ground electrode, fluidic tubes, etc. use distilled water and a syringe.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 10

9-4. ISE TROUBLE-SHOOTING BT3000 PLUS

(Quick Reference Guide to resolve main problems in ISE Module)

SYMPTOMS CAUSE AND CORRECTIVE ACTION

Bubbles in ISE 1) No prime performed after maintenance/service of

Module the module. Perform prime.

2) The solution is not aspirated, consequently it remains in the funnel. Obstruction in the Cl and

CO

2

electrodes column. Remove obstruction by observing appropriate cleaning procedure.

3) Worn peristaltic pump cartridge. Replace with new cartridge.

4) Funnel obstructed. Clean funnel.

5) Tubing sticking together in pinch-valve. Repair or replace.

Calibration Error.

High values of slope

K > 60, Na > 68 and unstable values.

1) Partially occluded Cl electrode. Clean electrode with appropriate tool.

2) A small obstruction in the Ground electrode. Clean electrode.

Calibration Error

Slope values at the limit of acceptance.

1) Reagent solutions not homogenized during refill.

Use homogenized solution for refilling.

2) Dirty funnel. Clean the funnel.

3) New electrodes still in the conditioning phase.

Inexact values in samples and slopes.

1) Erroneous STD solutions. Use correct solutions.

2) Inversion of Baseline with Buffer. Position correctly

Drift of values

Baseline and Buffer.

3) Empty Reference electrode. Pinch-valve tube problem. Repair or replace.

4) Use of contaminated distilled water of the analyzer.

Use non-contaminated distilled water.

1) Module just reconditioned. Repeat calibration 3 or

4 times.

2) Deteriorated Reference Electrode. Verify and replace.

3) The deproteinization of the electrode column has not been performed. Lack of washing at end of work. Observe appropriate washing procedure.

NOTE:

If the problem is still not resolved after all the preceding steps, then substitute the preamplifier or the entire ISE Module to resolve the problem.

Section - IX ISE Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 10

SECTION - X

COMPUTER BOX ASSEMBLY

Touchscreen

Power

Supply

ON/OFF

Switch

LCD Display

Module

DVD ROM

Player

Cabinet

Fan

Computer Module

Figure 10-1.

Serial

Board

External

Monitor

PS/2 Ports for

Keyboard & Mouse,

USB Ports etc.

Asus

Mainboard

Floppy Disk

Hard Disk

Section - X Computer Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 sur 5

10-1. ELECTRONICS MODULE

The LCD Display module, with fully integrated touch-screen, is on the front of the computer box. An on/off switch for the analyzer is located on the bottom left of the display. The peripherals, DVD-ROM player and the floppy disk drive are accessed through a cover just below the display.

All of the connections to the external peripheral devices are located on the rear of the box as follows:

1 USB Port for the Printer

1 Serial-Port for Host Computer

1 Serial-Port for Modem

1 USB Port for UPS

2 PS/2 ports for Keyboard & Mouse

The ASUS main-board with a 860 MHz Intel Pentium ® III processor, 128 Mb RAM,

DVD/CD-ROM player, 20 Gb hard disk, floppy disk drive, and the power supply are all located inside the computer box. Product enhancement resulting from new innovations in technology and from our continuing quality improvement effort may necessitate changes in this configuration in the future. However, any eventual changes will be fully compatible with the previous version. A connecting cable composed of lead conductors of the serial port and the 5 Volt supply interconnects the computer box and the process electronics.

1 PS/2 mouse connector (green) 6-pin.

1 PS/2 keyboard connector (purple) 6-pin.

Universal Serial Bus (USB) for Data Bus 1 & 2 (black) 2X4 pins.

2 Serial Ports Connector (turquoise green) com1 = 9-pins & com2 = 9-pins.

Parallel Port Connector (purple red) 25-pins Printer.

Section - X Computer Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 sur 5

Automatic turning off of the fan: during this process, the power supply to the fan will automatically switch to a standby mode. This function reduces both the energy consumption and disturbances in the system, and this is an important feature in implementing a silent system.

10-1.3. LCD MODULE 8-WIRE ANALOGUE RESISTANCE

TOUCH-SCREEN

The touch-screen interface enables functional operation of the program without the use of an external keyboard or mouse. The LCD module has a protective surface to prevent damage during data input or other operations.

10-1.4. POWER SUPPLY MODULE

Input Voltage Range - Electrical Specifications

PARAMETER MIN NOM. MAX Unit

V-input Range 90 115/230 264 V-rms

Output Voltage Range - Electrical Specifications

Output

Voltage

Nominal

Value

Regulation Ripple & Noise Output Current

Min Max.

Peak

1 +3.3V 16A

± 25A

5

6

3 +12V 0.2A

4 -5V ± 5% 100mV 0 A 0.3A

-12V

+5VSB

± 5%

± 5%

120mV

100mV

0 A

0 A

0.8A

2A

Note: The Connector meets AT standard specifications.

Section - X Computer Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 sur 5

10-1.5.

PS/2

T: Mouse

B: Keyboard

USB1

USB2

COM 1

COM 2

MIC2

LINE

OUT

LINE

IN

MIC

IN

AUX CD1

MOTHERBOARD LAYOUT CONTENTS

JTPWR

USB_PWR0

PWR_FAN

MODEM

CPU_FAN

Socket 370

VIA

VT82C694X

Chipset

Accelerated Graphics Port

PCI 1

PCI 2

PCI 3

PCI 4

PCI 5

WOL_CON

0 1 2 3

4 5 6 7

TUV4X

VIA

VT82C686A

Chipset

VIO

LED

DIP Switches

DIP_SW

USBPORT

WOR

CR2032 3V

Lithium Cell

CMOS Power

CLRTC

USBPWR1

JEN ASUS

ASIC

With Hardware

Monitor

CHA_FAN

SMB

Section - X Computer Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 sur 5

1

2

3

4

PS2KBMS

PS2KBMS

USB

PRINTER

External Connectors

PS/2 Mouse Port Connector (6-pin female)

PS/2 Keyboard Port Connector (6-pin female)

Universal Serial Bus Connectors 1&2 (two 4-pin female)

Parallel Port Connector (25-pin female)

5

6

7

COM1/COM2 Serial Port Connectors (two 9-pin/10-1 pin male)

GAME/AUDIO Game/MIDI Connector (15-pin female)

AUDIO Audio Connectors (three 1/8" jacks)

1

2

4

5

IDELED

FLOPPY

Internal Connectors

IDE Activity LED (2-pin)

Floppy Disk Drive Port Connector (34-1 pin)

DE Connectors (Two 40-1 pin)

SECONDARY IDE

WOL/CON

WOR

Wake-On-LAN Connector (3-pin)

Wake-ON-Ring Connector (2-pin)

CPU/PWR/CHA/FAN Chassis and CPU Fan Connectors (3-pin) 6

7

8

CHASSIS

SMB

9 IR

10 USBPORT

Chassis Intrusion Lead (4-1 pin)

SM Bus Connector (5-1 pin)

Infrared Module Connector (5-pin)

USB Header (10-1 pin)

11 ATXPWR

12 AFPANEL

13 MIC2

ATX Power Supply Connector (20-pin)

ASUS Panel Connector (12-1 pin)

Internal Microphone Connector (3-pin)

14 CD/AUX/MODEM Internal Audio Connectors (three 3-pin)

15 PWR. LED (PANEL) System Power LED Lead (3-pin)

16 SPEAKER (PANEL) System Warning Speaker Connector (4-pin)

17 MSG. LED (PANEL) System Message LED (2-pin)

18 SMI (PANEL) System Management Interrupt Lead (2-pin)

19 PWR. SW (PANEL) ATX/Soft-Off Switch Lead (2-pin)

20 RESET (PANEL) Reset Switch Lead (2 pins)

Section - X Computer Module BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 sur 5

SECTION - XI

POWER SUPPLY AC/DC MODULE

11-1. POWER SUPPLY

Lambda CA400 Series

Alpha power supply AC/DC:

Universal Input voltage range:

12MIN, 24D, 24DRP

85 to 264 ~

Frequency: 47 Hz to 63 Hz

Modular configurable single and multiple outputs:

CONFIGURED OUTPUT VOLTAGES

Output Voltages (preset) Adjustment Range Output Current

12 Volts

24 Volts

24 Volts

12 Volts

24 Volts

18-29 Volts

8A

8A

8A adjustable

11-1.1. EMC DIRECTIVE APPLICATION TO THE POWER

SUPPLIES

The EMC directive does not apply to power supplies component. It is impossible for the manufacturer of the power supply unit to guarantee conformity of the final product to this EMC directive. The EMC behavior of the power supply can be considerably affected by the way it is installed in the users system. However, since the power supply is a major component in the performance of the final product, in regards to this Directive, it is indispensable that the power supply manufacturer ensures that the power supply enables the end user to conform to the EMC Directive in the most effective manner.

Section - XI Power Supply BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 2

11-1.2. POWER FACTOR CORRECTION (PFC)

Certain applications have a legal requirement for harmonic reduction, as found with in-vitro diagnostic devices. Power Factor Correction provides the advantage of wide range input, and maximizes the load that can be taken from the main power sources.

11-1.3. INHIBIT OPTION (INH)

The inhibit option is provided as a board inside the module that can reduce the output voltage of 12 Volts to less than 0.5 Volts by application of a TTL compatible control signal. This circuit is inhibited through the reset signal. In this way the voltage supply to the analyzer lamp is cut off, while interrupting power to the solid state relay located on the Control Board (15-04431-01), thus interrupting the mains voltage supply to the peripherals (printer and vacuum pump).

Section - XI Power Supply BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 2

SECTION XII

VACUUM PUMP SYSTEM P/N 06-05161-01

OPERATING INSTRUCTIONS

Analyzer

Blue Tube

Vacuum Pump System

Always check and empty the full waste container and press "Restart" button

Restart

To Analyzer

Waste Probe

Normal Pressure

Low Pressure

Low-Pressure Alarm

WARNING

1) Read vacuum pump system

instruction and warning

notices carefully

2) Disposable gloves must be worn when

servicing the vacuum pump system

where hands may contact potentially

contaminated waste materials biotecnica instruments

Analyzer

Black Tube

12-1.

Figure 12.1

Vacuum Pump System P/N 06-05161-01 for BT3000 PLUS

This silent and compact vacuum pump system has been specifically designed for use with BT3000 PLUS type analyzers. It provides for an automatic and safe collection of waste liquids from analyzer’s reaction cuvettes and sampling needle washing into the external waste disposal container in lab environment. The system consists of a metallic cabinet containing two membrane pumps controlled by a microprocessor, electronics, and the electrical and fluidic connections to the analyzer. The microprocessor manages 3 main functions: pressure measurement, waste container full check, and pump shutdown. The waste discharge has the following pathway (course):

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 1 of 26

The liquids aspirated by the pumps from the needle washing funnels (bowls) and the cuvettes washing through the two tubes (Blue and Black tubes) respectively are transferred to an internal waste chamber (made of transparent Pyrex). From here the waste fluid is ejected to the external waste container through the waste probe equipped with liquid level sensor.

Whenever the external waist container is full the waste-probe liquid level sensor activated audio/visual alarm alerts the operator and instantly shuts down the pumps.

12-1.1. SYSTEM CONTROL FUNCTIONS

Pressure: Three LEDs on the front panel indicate the system vacuum level.

Green LED: Normal operating vacuum level (approximately –60 millibar).

Yellow LED: Low vacuum level (below 40 millibar).

Red LED: Vacuum level lower than –15 millibar

Alternating Green, Yellow, and Red LEDs: Indicate high vacuum level

(higher than –300 millibar).

All LEDs turned off: Pumps disabled.

The green LED indicates that the system is functioning properly.

The yellow LED may indicate an operating limit condition, still satisfactory but signalling the beginning of vacuum level deterioration. The red LED indicates vacuum level failure due to various reasons.

The alternate lighting of Green, Yellow, and Red LEDs indicate excessive vacuum level probably caused by an occluded waste probe tube (from the vacuum pump system to the external waste container.

All LEDs turned off condition is reached when the standby time (2 minutes) terminates without resetting the system (pumps disabled). In this case the green

LED of the Restart button flashes in quick successions.

The green LED also confirms the power on condition of the vacuum pump system. If the green LED is not lit then this may be due to either the power cordset of the vacuum pump system is disconnected from the analyzer or the vacuum pump system malfunction.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 2 of 26

12-1.2. DESCRIPTION OF THE BOARD 15-05085-01

(VACUUM

The Board 15-05085-01 controls the vaccum pump system operation.

The microcontroller (µC) U2 (87LPC767) in OTP version monitors all the functions of the system. It controls the signals from level sensor of the waste probe, two pumps in DC, 3-way solenoid valve, pressure sensor SP1, and generates acoustic alarms at two distinct frequencies.

The connector J1 allows connection to the power supply, which supplies 24Vdc.

The voltage of 5Vdc is obtained from the 24Vdc through the low dropout regulator

U1 (L4931 CV50).

The connectors J2 and J3 connect the circuit to the two 24Vdc membrane pumps dedicated to the aspiration of the waste liquids.

The connector J4 allows connection for the signals from the LEDs, and the maximum level limit of the waste probe level sensor.

The connector J5 is utilized for transmitting alarm signals to the analyzer (for

TARGA BT3000 and by-products only).

The connector J6 connects the circuit to the 3-way solenoid valve, which deviates waste liquids to the internal waste bottle in the vacuum pump system when the external waste container is full.

The connector J7 is provided for an eventual pressure measurement device.

The bit P1.7 of the U2 controls the Buzzer BZ1. Depending upon the alarm function, it generates two distinct audible alarms as follows:

1)

2)

Frequency of 1 beep per second.

Frequency of 2.5 beeps per second.

The green LED diode DL1 is lit in normal operating conditions (pressure > 30 millibar) and is monitored by the gate P1.4 of the U2.

The yellow LED DL2 lights up when the pressure is in the range of 20 - 30 millibar. This range indicates pre-alarm condition. The gate dedicated to this is

P0.2 of U2.

The red LED diode DL3 lights up to indicate low-pressure alarm condition < 16 millibar. The command gate is P0.6 of U2.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 3 of 26

The MOSFET Q1 is used for supplying or switching off voltage to the two membrane pumps connected in parallel. The command bit arrives from the gate

P1.0 of U2.

The pushbutton SW1 is dedicated to the Restart function of the system. When the green LED inside the pushbutton is lit, it indicates that the vacuum pump system is turned on. The blinking green LED in the pushbutton indicates that the system requires restarting.

The gate P0.4 of the U2 controls the Darlington transistor Q2. It activates or inactivates the 3-way solenoid valve for waste liquids management of external waste container or the internal waste bottle in the vacuum pump system.

The pressure sensor SP1 supplies a voltage proportional to the operating pressure of the vacuum pump system. An appropriately amplified signal from the integrated circuit U4 is transmitted to the gate P0.3 of U2.

12-1.3. VACUUM PUMP SYSTEM P/N 06-05161-01

COMPONENT FUNCTIONS

System Overview

This paragraph provides a description of the functions of the main components of the Vacuum Pump System P/N 06-05161-01.

The Vacuum Pump System consists of four main components inside the cabinet: an AC/DC power supply with universal input 95-240 VAC and 24VDC output, the

Controller Board 15-05085-01, two membrane pumps, and a 3-way solenoid valve.

CIRCUIT FUNCTIONS:

Pressure monitoring

Only the green LED (DL1) should be lit (indicating nominal operating pressure) during the normal operation of the vacuum pump system.

The lighting up of yellow LED (DL2) indicates that the operating pressure has gone below 30 millibar (it can be acceptable if this occurred now and then).

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 4 of 26

If the red LED (DL3) lights up then this indicates that the operating pressure has gone below 16 millibar (inadequate for normal analyzer operation). An audible alarm of 1 beep per second frequency is generated after 6 seconds.

In particular cases (e.g. when large amount of liquid is aspirated) it is possible that all three LEDs (on the front panel of the pump cabinet) are lit intermittently. This indicates the presence of high pressure (more than 200 millibar) in the waste bottle located inside the pump cabinet. If this persists for more than 10 seconds then the "Restart" pushbutton LED illuminates generating an audible alarm at the frequency of 2.5 beeps every 10 seconds.

Waste Container control

The waste probe is placed into the external waste container to transfer liquid ejected from the waste pumps into the container. For safety reasons the waste probe is equipped with a liquid level sensor, which is activated, when the external waste container is full. In this condition the liquid level sensor transmits the signal to the microprocessor, thus putting the system in standby mode. In addition the vacuum pump system generates an intermittent audible alarm of low frequency (1 beep per second), and simultaneously the red LED on top of the waste probe and the LED in the "Restart" button start flashing. In the mean time the system continues to function for about 100 seconds as the waste liquids are deviated to the internal waste bottle through the 3-way solenoid valve, thus continuing the waste aspiration function transitorily but the waste flow to external waste container is stopped. The purpose of this momentary deviation is to avoid any spillage during the transfer of the waste probe from one waste container to another empty container. After emptying or substituting the external waste container press momentarily the "Restart" button on the vacuum pump system front panel to continue the operating procedure and reset the alarm. If the preceding step is not taken, after approximately 100 seconds the aspiration pumps stop running, the three LEDs on the cabinet front panel are switched off and an audible alarm of 2.5 beeps per second frequency is generated. After additional 10 seconds the 3-way solenoid valve is de-energized.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 5 of 26

NOTE: The liquid level sensor is magnetically actuated reed switch make and break type.

Vacuum Pump System reset

If due to the above-mentioned reasons the vacuum pump system enters the alarm condition the "Restart" button LED starts flashing, simultaneously the analyzer enters the standby mode and a corresponding warning message "LOW

PRESSURE" appears on the screen. Empty or substitute the external waste container and momentarily press the "Restart" pushbutton to reset the vacuum pump system. The vacuum pump system starts functioning and the internal waste bottle is emptied. After reaching the correct operating pressure all the normal operating controls and alarms are restored.

12-1.4. INSTALLATION & OPERATION

1) Connect the Blue and Black waste tubes from the right side of the analyzer to the appropriate quick connect on the vacuum pump cabinet front panel.

3) Connect the Waste Probe electric cable (liquid level detector) and the drain tube of to the vacuum pump cabinet front panel.

4) Insert the Waste Probes in authorized external waste container.

5) Plug the power cordset to power inlet on the rear of vacuum pump cabinet and then into one of the accessory power connectors on the analyzer rear panel.

6) Now the system is fully installed and ready for operation.

7) Switch on the analyzer. The vacuum pump will start running

NOTE:

The vacuum pump system power inlet on the rear has no ON/OFF switch. The system turns ON and OFF simultaneously with the analyzer. The vacuum Pump

System is equipped with a universal power supply of 90 to 240 Volt AC, 50/60 Hz similar the power supply of BT3000 PLUS.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 6 of 26

12-1.5. MAINTENANCE AND CARE

This compact pump system is virtually maintenance-free and offers continuous duty collection of waste liquids outside the analyzer. Does not require inconvenient peristaltic pump cartridge and filter changes. The theoretical operating life of the pumps is between 4000 to 5000 hours, after that the service technician should substitute the pumps.

WARNING a) DISPOSABLE GLOVES MUST BE WORN WHEN SERVICING THE VACUUM PUMP

SYSTEM WHERE HANDS MAY CONTACT POTENTIALLY CONTAMINATED WASTE

MATERIALS. b) THE SAFE DISPOSAL OF THE WASTE MATERIALS IS THE RESPONSIBILITY OF

THE USER. INSURE THAT THE DISPOSAL OF WASTE CONTAINER FLUIDS IS

DONE ACCORDING TO ALL APPLICABLE LAWS AND REGULATIONS.

12-1.6. TROUBLE-SHOOTING

TROUBLE-SHOOTING GUIDE

The vacuum pump is silent and

"Restart" green LED not lit.

Audible alarm of intermittent frequency, some flashing LEDs and the instrument in standby mode.

Audible Alarm of intermittent frequency with the LED on the waste probe lit.

Damaged Unit

Smoke, strange odor or noise a) No power to the system. Power cord disconnected. Connect the power cord. b) Blown fuse/s. Replace with appropriate fuses as marked.

Indicates vacuum level variation due to leakage in the hydraulic circuit connection/s. Ensure that the waste tubes (blue and black tubes connecting vacuum pump to the analyzer) are firmly connected.

During the analyzer operation this phenomenon may be considered normal, as there may be sudden variations in the vacuum level during emptying of the reading cuvettes.

External waste container full. Empty the external waste container and press “RESTART” button on the vacuum pump cabinet to continue the operating procedure and to reset the alarm.

The unit has suffered severe shock such as being dropped. Do not use the unit. Consult your nearest sales/service office regarding repair.

Stop using the unit immediately and disconnect the power supply. Consult your dealer regarding repair.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 7 of 26

Blue Tube

Vacuum Pump

System

Aspiration Pump

Internal

Waste Chamber

External Waste

Container

ANALYZER

Fluidic Pathway

Figure 12.2

Black Tube

Power Cord

Waste Probe

ELECTRIC CABLE

DRAIN TUBE CONNECTOR

LEVEL

SENSOR

RED LED

Waste Probe (P/N. 07-05165-01)

Figure 12-3

12-1.7. SPARE PARTS FOR REPAIR AND MAINTENANCE

The following is listing of subassemblies and individual parts, which are available for field replacement:

PART NO. DESCRIPTION

06-05161-01 VACUUM PUMP SYSTEM (COMPLETE) FOR BT3000 PLUS

08-05162-01

15-05085-01

20-05145-01

330.6312

330.6338

330.6400

VACUUM PUMP ASSY

VACUUM PUMP SYSTEM CONTROLLER BOARD

INTERNAL WASTE CHAMBER (TRANSPARENT GLASS)

POWER ENTRY MODULE

FUSE 250 VOLT, 0.5 AT

MAIN POWER SUPPLY CORDSET

330.9099 3-WAY SOLENOID VALVE

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 8 of 26

VACUUM PUMP SYSTEM P/N 662.0788/C/D/E/F

(OBSOLETE)

No longer in production

12-2. INTRODUCTION

This compact and silent waste suction unit has been specifically designed for use with the BT3000 PLUS chemistry analyzer where continuous elimination of liquid is required to be automatic removal of waste. The gauge-controlled suction can reach a maximum vacuum of -400 mbar. It produces low-noise level while maintaining strong suction capabilities. It provides for an automatic and safe collection of waste liquids from to the external waste disposal containers in laboratory. Whenever the external waist container is full the waste-probe liquid level sensor activates a low frequency audio/visual alarm and instantly shuts down the peristaltic pump. The vacuum pump stops running, if any of the internal waste collection chamber is full and remains in the standby mode until the waste containers are emptied and the alarm reset.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 9 of 26

The suction unit cabinet accommodates two inner waste collection chambers of approximately 300ml capacity each. The vacuum to the waste chambers is aspiration of waste liquids from analyzer is provided by the vacuum pump. The waste liquid, aspirated from the analyzer is collected in these waste chambers.

Simultaneously, two high throughput peristaltic pumps are used to transfer the waste liquid to the external waste containers through external waste probes, thus preventing secondary contaminant exposure. The peristaltic pump features a removable and disposable cartridge that is pre-loaded with special Pharmed tube.

12-2.1. VACUUM GAUGE

The vacuum status is clearly indicated on the front panel vacuum gauge.

12-2.2. VACUUM LEVEL REGULATOR

The pump circuit incorporates an automatic vacuum level regulator, which is factory set at -400 mbar (-40 kPa, -300 mm Hg, -11.8 in Hg). The vacuum regulator monitors the vacuum level and automatically adjusts it when the vacuum level rises above the desired point, in the event of clogged filter or any other system occlusion. By using the vacuum regulator the risk of a pump failure due to excessive is eliminated, extending the pump life and ensuring high performance.

12-2.3. HYDROPHOBIC FILTER

A disposable hydrophobic filter protects the vacuum pump from contamination If the filter becomes clogged it may be replaced any time. The hydrophobic filter captures superfine particles, bacteria, condensate, and any liquid that may contaminate or damage the pump.

PUMP SYSTEM

To protect the vacuum pump from vibration and impact during shipment, it comes installed with a clamping device. When you unpack your vacuum pump system, make sure to remove two transit screws (colored red) at the bottom of cabinet to release the vacuum pump. Running the pump with the transit screws installed will cause an increased noise level during operation and may damage internal mechanisms.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 10 of 26

12-2.5. INSTALLATION PROTOCOL

Refer to Figure 1 entitled "Vacuum Pump System Installation" before attempting to install the analyzer. Install all electrical cables and tubes as follows:

1) Remove the transit screws at the bottom of the pump cabinet.

2) Connect waste tubing from the right side of the analyzer to the appropriate quick connect connectors on top of the vacuum pump cabinet.

3) Connect the liquid level detector cables and the drain tubes of Waste

Probes to the front panel of the vacuum pump.

4) Insert the waste probes in the appropriate external waste containers.

5) Plug one end of the power cord to the power inlet on the vacuum pump cabinet and the other end to the accessory power connectors on the analyzer rear panel.

12-2.6. QUICK START-UP

1) Switch on the analyzer. The vacuum pump will start running.

2) Maximum operating vacuum is preset at –400 mbar ± 10%.

3) The vacuum level during unit's normal standby/operating mode may vary between –100 mbar (-75 mmHg) to -300 mbar (-225 mmHg). In case the vacuum level drops below –100 mbar then refer to Paragraph 12-1.14. entitled "Trouble-Shooting Guide".

Note: The waste probe LED and audible alarms keep you aware of the unit’s status as follows: a) 1 st alarm (Beep of 1-second frequency):

The LED on the waste probe will be lit. The external waste container is full. Empty it. In the mean time vacuum pump continues to function without blocking the analyzer for about 4 minutes as the waste chamber inside vacuum pump system is being used. It is important to press the

“RESTART” button on the vacuum pump cabinet to continue the operating procedure and to reset the alarm. b) 2 nd alarm (Beep of 0.5 second frequency):

The LED on the waste probe will be lit: This alarm is activated when the external waste container is not emptied and the waste chamber inside the vacuum pump system is full. A signal is sent to the analyzer to interrupt the operating functions with a "Waste Bottle Full" message. c) The “Waste Bottle Full” message will stop analyzer function. Once the cause of the message is remedied, the command “Start Another Tray“ can be selected to carry on the operating procedure with the analyzer.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 11 of 26

12-2.7. CARE AND MAINTENANCE

For this care and maintenance section, refer to Figures 12-2, 12-3, and 12-4. It is highly recommended to replace the Peristaltic Pump Cartridge P/N 330.9088 and the Hydrophobic Filter P/N 662.0807 every 6 months.

!

CAUTION

a) HIGH VOLTAGE INSIDE! HAZARD OF ELECTRICAL SHOCK. PUMP

WIRING IS LIVE. DISCONNECT INPUT POWER (POWER CORD)

BEFORE SERVICING. NEVER OPERATE WITHOUT SECURE

GROUND CONNECTION. FAILURE TO DO THIS MAY CAUSE

ELECTRIC SHOCK OR INJURY. b) THE VACUUM PUMP SYSTEM MUST NOT BE DISMANTLED OR

REPAIRED BY ANYONE WHO HAS NOT BEEN QUALIFIED TO DO

SO BY THE MANUFACTURER. INCORRECT WORK MAY CAUSE

FIRE OR IRREPARABLE DAMAGE TO THE VACUUM PUMP

SYSTEM.

12-2.8. FUSE REPLACEMENT FOR MAIN POWER INLET MODULE

The mains power inlet module has a built-in fuse holder and two fuses of 250 Volt,

2AT (P/N 330.6340) for 230 VAC pump or 250 Volt, 4 AT (P/N 330.6342) for 115

VAC pump. Fuses protect the system against power surges or overload by interrupting power. Replace fuse/s as marked on the rear panel. To replace a fuse proceed as follows:

1) Disconnect the power cord. Remove the fuses by gently extracting the fuse-holder with a tool. The fuse-holder has two grooves on the sides for tool accessibility only.

2) Discard the old fuses and replaces with new fuses that matches the selected voltage rating indicated on the back panel label. Insert the fuseholder into the compartment and push until the latch snaps back into position.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 12 of 26

12-2.9. FILTER REPLACEMENT

1) Disconnect the power cable.

2) Loosen the cover screws and remove cover.

3) Remove the filter tubing (blue) from the waste chamber and the vacuum pump quick connects. Discard the filter.

4) Install a new filter by connecting longer tubing (filter outlet) to the quick connect on the vacuum pump. It is important that the arrow indicator ( →) on the filter point towards the air intake of the pump.

5) Connect the shorter feed tubing to the quick connect on the waste chamber. Install the cover and tighten screws.

NOTE: The hydraulic circuit, inside the pump cabinet, accommodates quick-connect fittings for the reliable connection and the prevention of accidental release. The approved tubing for quick-connect must always be cut clearly at 90° angle. To connect or disconnect observe the following simple steps: a) To connect simply push tubing into the collar past the O-ring to the b) internal tube stops.

To disconnect press collar against the fitting body and slide out tubing.

12-2.10. PERISTALTIC PUMP CARTRIDGE REPLACEMENT

The peristaltic pump features removable cartridge pre-loaded with special

Pharmed ® tube. To replace cartridge proceed as follows:

1) Remove both ends of the peristaltic tubing with elbow inserts from the quick-connect couplings. Remove elbow inserts.

2) Squeeze the locking catches and remove the defective cartridge and discard it.

3) Introduce elbow inserts into the pump tubing. Place the new cartridge on the drive shaft and gently press to snap fit.

4) Carefully connect the pump tubing to both quick-connect on the front panel. The pump is now ready for operation.

NOTE: The disposable cartridge is based on snap-fit principle. As the cartridge is put in place and locked, the three rollers within the cartridge engage the unit's drive shaft. To connect coupling simply plug insert into body. The fitting halves lock in place instantly with an audible "click", which assures a reliable and leak-free connection. To disconnect the fitting halves, simply press the thumb latch (lever) on the coupling body and the coupling halves separate.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 13 of 26

12-2.11. VACUUM REGULATION PROCEDURE

The vacuum limit valve is located on the internal right waste chamber. This modulating valve protects the pump from “maximum vacuum” (the highest vacuum the pump can attain when the pump inlet is closed). In the event of clogged filter or any system occlusion, the vacuum regulator eliminate possible trouble or burnout caused by tentative overload. The valve maintains a constant predetermined vacuum level and will automatically respond to any rise of vacuum level above the valve setting. The following procedure should be observed when calibrating the vacuum limit valve:

1) Disconnect the power cable. Disconnect the waste tubes with elbow inserts (Blue and Black) from the quick-connect couplings on the right side of analyzer.

2) Loosen the cover screws and remove cover. Turn on vacuum pump system.

3) Loosen the lock nut securing the vacuum regulating cap (this is the vacuum regulator located on the right chamber).

4) Carefully turn the vacuum regulator cap clockwise or counterclockwise until the vacuum gauge needle points to the recommended set point –400 mbar (-40 kPa, -300 mm Hg, 11.8 inch Hg, -5.8 PSI) The preset vacuum level clearly reflects the vacuum level existing in the suction line.

NOTE: To reduce the vacuum level, turn the regulator cap counterclockwise. To increase the vacuum level, turn the regulator cap clockwise. When adjusting valve, make no more than one turn of the cap at a time and observe the vacuum level closely to prevent overshooting the specified setting. As much as 1 minute may be required for the new balance to take place after the adjustment is made.

Tighten the lock nut against regulating cap while firmly holding the cap in position.

Connect waste tube to the rear of analyzer. Start the Vacuum Pump System and verify proper operation.

12-2.12. PRINCIPLES OF OPERATION

The fluids aspirated from the analyzer are separated according to degree of contamination into 2 separate channels. Basically the blue tube evacuates minimally contaminated fluids while the black tube evacuates highly contaminated fluids. The internal waste chamber receives the fluid and is not emptied by the peristaltic pump until the rising liquid level (few centimeters) actuates the bottom liquid level sensor.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 14 of 26

The liquid level sensor controls the peristaltic pump until the liquid level returns to the minimum position. The liquid level sensor is used for operating the peristaltic pump when necessary.

The liquid level sensor located on the top of waste chamber is for signaling any system error. If the peristaltic pump were to malfunction, the waste chamber would continue to fill up until the liquid overflows into the vacuum pump. To prevent this from happening, the upper liquid level sensor is actuated. This disables the system by turning off the vacuum pump. Instantly an audible alarm is activated, with intermittent acoustic signals of high frequency.

The waste probe transfer the liquid ejected from the peristaltic pumps into the external waste containers. For safety reasons each waste probe is equipped with a liquid level sensor which is activated when the external waste container is full. In this situation, the vacuum pump system generates an intermittent audible alarm and a visual red LED would be lit on top of the waste probe is lit.

12-2.13. TECHNICAL DESCRIPTION BOARD (15-04541-01)

The board 15-04541-01 controls and monitors the whole vacuum pump system.

The µC764, in the OTP version, controls all the functions of the system. In addition this micro-controller controls the signals of the liquid level switches, activates the peristaltic pumps and generates acoustic alarms.

The J1 connector is used to connect the power supply to obtain 24 VDC. The reduction to 5 VDC voltage is derived from 24 VDC supplied through the U1 voltage regulator (LM7805). The J2 connector is used to connect the circuit to the liquid level sensors inside the two waste chambers. The rising level float switch is connected in OR. When the liquid level inside the waste chambers reaches the high actuation level (upper liquid level switches), the associated liquid level switches from closed to open. In case the liquid level in the waste chamber is below the low actuation level, the bottom float switches from open to closed contacts.

The Q1 and Q2 transistors are the power drivers of the peristaltic pumps. The J3 connector permits connection to the main AC power. The vacuum pump is connected to the J4 connector. The J5 connector is used to connect the circuit to the peristaltic pumps, waste probes, and the "Restart" push button.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 15 of 26

The various gates of the integrated U3 circuit (ULN2803) control the following functions:

• The U3A gate activates the red LED (visual alarm) on the Waste Probe-2.

• The U3B gate activates the red LED (visual alarm) on the Waste Probe-1.

• The U3C gate activates acoustic alarm through BZ1 buzzer.

• Gates U3D, U3F, and U3H control the relay K1 to turn off power to the vacuum pump in case of error in the system.

The jumpers 1, 2, and 3 allow the utilization of the electronic circuit in the following mode: a) Jump 1 shorted for BT3000 PLUS; open for BT3000 and by-products. b) Jump 2 shorted for BT3000 PLUS; open for BT3000 and by-products. c) Jump 3 shorted for BT3000 PLUS; open for BT3000 and by-products.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 16 of 26

12-2.14. Trouble-Shooting Guide

A 1-second audible with the

LED on the waste probe lit.

Waste Bottle Full message.

A 0.5-second audible with the

LED on the waste probe lit.

The vacuum level below

–100 mbar (-75 mmHg)

Damaged Unit

Liquid such as water has been sucked into the vacuum pump

1. External waste container full. Empty the external waste container and press

“RESTART” button to reset.

2. Remedy the causes of alarm and then enter the command “Start Another Tray“ to carry on the operating procedure with the analyzer.

NOTE: The vacuum pump continues to function without blocking the analyzer for about 4 minutes as the waste chamber inside vacuum pump system is being used. It is important to press the “RESTART” button on the vacuum pump cabinet to continue the operating procedure and to reset the alarm.

The external waste container is not emptied and the waste chamber inside the vacuum pump system is full. In this case a signal is sent to the analyzer to interrupt its functions. Empty the external waste container. Press the “RESTART” button to reset the alarm.

1. In the normal operating conditions the vacuum range may vary between -100 mbar

(-75 mmHg) to -300 mbar (-225 mmHg). The vacuum level below -100 mbar (-75 mmHg) indicates leakage in the hydraulic circuit.

Ensure that the waste tubes (the blue & black tubes connecting vacuum pump to the analyzer) and the exhaust tubes in the analyzer are firmly connected.

2. Partially occluded hydrophobic filter located inside vacuum pump system. Replace with

Part No. 662.0807.

Caution: Always install filter with the flow (flow directional arrow pointing towards the pump) entering the vacuum pump from the waste chamber side.

The unit has suffered severe shock such as being dropped. Do not use the unit. Consult your dealer regarding repair.

Stop using the unit immediately and disconnect the power supply. Consult your dealer regarding repair.

Smoke, strange odor or noise Stop using the unit immediately and disconnect the power supply. Consult your dealer regarding repair.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 17 of 26

12-2.15. SPARE PARTS FOR REPAIR AND MAINTENANCE

The following is listing of subassemblies and individual parts, which are available for field replacement:

PART NO. DESCRIPTION

15-04541-01 VAC.PUMP SYSTEM CONTROLLER BOARD (FIG. 12-2., 12-4.)

330.6312 POWER ENTRY MODULE WITH FUSES (FIG. 12-2., 12-4.)

330.6340

330.6342

FUSE 250 VOLT, 2 AT ( 230 VAC VACUUM PUMP SYSTEM)

FUSE 250 VOLT, 4 AT ( 115 VAC VACUUM PUMP SYSTEM)

330.6400

330.7175

330.9088

330.9622

330.9623

330.9624

MAIN POWER SUPPLY CORDSET

POWER SUPPLY (FIG. 12-2., 12-4.)

CARTRIDGE # PERISTALTIC PUMP (FIG. 12-2., 12-4.)

COUPLING BODY WITH SHUTOFF VALVE - FRONT PANEL (FIG.

12-2.)

ELBOW FITTING FOR PERISTALTIC PUMP CARTRIDGE(FIG. 12-4.)

330.9625

330.9649

COUPLING BODY STRAIGHT THROUGH FRONT PANEL (FIG. 12-

2.)

SINTERED BRONZE SILENCER (FIG. 12-2.)

COUPLING INSERT FOR WASTE PROBE - FRONT PANEL (FIG. 12-

2.)

VACUUM REGULATOR (FIG. 12-2.) 330.9659B

662.0777B

662.0777C

662.0788

662.0788A

662.0788B

662.0788E

VACUUM PUMP 115 VAC WITH FITTINGS (FIG. 12-2., 12-4.)

VACUUM PUMP 230 VAC WITH FITTINGS (FIG. 12-2., 12-4.)

VACUUM PUMP SYSTEM (COMPLETE SYSTEM WITH 2 WASTE

PROBES– 230 VAC

WASTE PROBE-1 (FIG. 12-5.)

WASTE PROBE-2 (FIG. 12-5.)

VACUUM PUMP SYSTEM (COMPLETE SYSTEM WITH 2 WASTE

PROBES– 110 VAC

"RESTART" PUSH BUTTON (NOT WIRED) 662.0806

662.0807

662.0808

662.0808A

HYDROPHOBIC FILTER ASSEMBLY WITH VAC PUMP (FIG. 12-2.)

PERISTALTIC PUMP - LEFT (FIG. 12-2., 12-4.)

PERISTALTIC PUMP - RIGHT (FIG. 12-2., 12-4.)

662.0810

662.0831

662.0831A

662.0831C

662.0831D

662.0831E

662.0831F

662.0831G

662.0831H

662.0832

662.0832A

VACUUM GAUGE ASSY (FIG. 12-2., 12-4.)

CABLE FOR POWER SUPPLY/BOARD 15-04541 (FIG. 12-6.)

CABLE FOR POWER SUPPLIES (FIG. 12-6.)

FEMALE FISCHER CONNECTOR KIT (FIG. 12-6.)

"RESTART" PUSH BUTTON KIT (FIG. 12-6.)

GROUNDING CABLE FOR VAC PUMP (FIG. 12-6.)

GROUNDING CABLE FOR POWER INLET (FIG. 12-6.)

GROUNDING CABLE FOR POWER SUPPLY (FIG. 12-6.)

GROUNDING CABLE FOR PUMP COVER (FIG. 12-6.)

THE INTERNAL WASTE CHAMBER-LEFT (FIG. 12-2., 12-3.)

THE INTERNAL WASTE CHAMBER-RIGHT (FIG. 12-2., 12-3.)

IMPORTANT NOTICE: Prior to any internal repairs or transportation, continue pressing the

“RESTART” button until the waste chamber inside the cabinet is completely emptied

(approximately 30 seconds) and the pump beeps continuously.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 18 of 26

12-2.16. TECHNICAL SPECIFICATIONS

Technical Specifications

Main Voltage.......................... 230 VAC, 50 Hz or 117 VAC, 60 Hz (As per Clients disposition)

AC-DC Power Supply

Input Voltage Range:..............

Input Frequency:.....................

Output Voltage Range:............

Operating Temperature

100-240 VAC, Switched Mode

(AC) 47-63 Hz

24VDC ± 10%

0 to +50°C

Vacuum Pump

Pump Type:............................

Rated Voltage:........................

Operating Vacuum ± 10%:......

Vacuum Level:.......................

Attainable Vacuum (Max):.......

Peristaltic Pump

Drive:.......................................

Motor Speed:..........................

Flow Rate:...............................

Tube Material and Bore:.........

Transmission:.........................

Media:.....................................

Life Expectancy:......................

Linear-motor driven free piston, oil-less

230VAC 50 Hz or 115VAC 60 Hz (As per Clients disposition)

Varies between -100 mbar (-75 mmHg) to -300 mbar

(-225 mmHg).

Automatically regulated by Vacuum Limit Valve factory set at -400 mbar (-300 mmHg). The valve is located downstream of the Hydrophobic Filter.

-800 mbar, -80 kPa, -600 mmHg, -23.6 in. Hg

Direct Current Motor 24 VDC

3000 RPM

110 ml/min

Pharmed; Bore: Ø3.2 mm

Friction transfer at minimal speed directly onto the rollers.

Analyzer waste

NOTE: It is recommended that the Pump Cartridge be changed every 6 months.

Hi-Lo Level Sensor:.............. Liquid Level Float Switch with hermetically sealed magnetic Reed Switch and permanent magnet.

Filter:......................................

Life expectancy:......................

Dimensions (Approx.)

Height:.....................................

Width:......................................

Depth:......................................

Weight:....................................

Disposable hydrophobic/bacteria filter

NOTE: It is recommended that the filter be changed every 6 months.

310 mm (12.2 inches)

230 mm (9.1 inches)

420 mm (16.54 inches)

15 kg (33.1 lbs)

EMC:....................................... Meets EN 55011, EN 50082-1 For conducted and radiated emission.

Electrical Standards:............. Conforms to Council Directives 98/79/EEC of 27 October

1998.

Warranty:................................ 1 year including parts and labor, excluding Consumables

The Biotecnica Instruments S.p.A. reserves the right to alter technical specifications without prior notice.

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 19 of 26

12-2.17. CLEANING OF THE HYDROPHOBIC FILTER

(Vacuum Pump System #BT3000 PLUS & BT2000/3000 TARGA)

Normally during the routine maintenance, the hydrophobic filter, located inside the vacuum pump system, is replaced approximately every 12 months.

Sometimes the filter may become occluded after a short period of time or suddenly.

These occlusions are generally caused by the presence of excessive foam formed in the waste liquid by agitation. This can happen if an excessive amount of surfactant detergent is used in the washing liquid or reagents containing too much surfactant. Even if there is a waste chamber in the vacuum pump system to attenuate the problem, the foam may overflow from the waste chamber into the hydrophobic filter, before the liquid level detector turns off the vacuum pump.

Observe the following procedure to resolve this problem:

1) When the external waste container is full, the system generates an audio-visual alarm. The operator must intervene soon to avoid overfilling of internal waste chamber causing foam to overflow into the filter.

2) Regeneration of the filter through cleaning. This operation is possible if the filter is occluded recently and has not exceeded its current operating life. In practice an old filter with membrane occluded by dried up residues, must be substituted.

3) Disconnect the power cable. Remove the cover from the vacuum pump system.

Remove the filter tubing from the internal waste chamber and the vacuum pump quick connects (metallic). Turn the filter upside down with the flow arrow indicator (⌦ ) on the filter (Fig. A) must point upwards. Remove the vent cap

(Fig. A) at the bottom of the filter and drain any liquid inside the filter. Use a plastic pipette on the top (opposite to flow arrow indicator) to blow out any liquid or wet residues from the clogged filter membrane. Now about 90% of the filter efficiency must have been restored. Best results can be obtained by using compressed air in place of the pipette.

4) Replace vent cap (Fig. A) and tighten it. Install the filter and the vacuum pump system cover and tighten screws.

Vent Cap

(Tappo uscita liquido)

Apply Plastic Pipette or Compressed Air

(Applicare Pipetta Plastica o Aria Compressa)

Figure A

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 20 of 26

A N A L L IZ A T O R E

(ANALYZER)

TUBO TRASPARENTE

(TRANSPARENT TUBE)

V a c u u m P u m p S y s t e m

I n s t a l l a t i o n

Figure 12-4

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 21 of 26

Hydrophobic Filter

P/N 662.0807

Power Entry Module

P/N 330.6342

Power Supply

P/N 330.7175

Controller Board

P/N 15-04541-01

Waste Chamber (L)

P/N 662.0832

Waste Chamber (R)

P/N 662.0832A

Coupling Body with

Shut Off Valve

P/N 330.9622

Coupling Body with

Shut Off Valve

P/N 330.9622

Coupling Insert

P/N 330.9649

Coupling Body

Straight Through

P/N 330.9624

Restart

Push Button

Vacuum Pump

P/N 662.0777C (230 Vac)

P/N 662.0777B (115 Vac)

Vacuum Regulator

P/N 330.9659B

Vacuum Gauge

P/N 662.0810

Silencer

P/N 330.9625

Peristaltic Pump - Left

P/N 662.0808

Peristaltic Pump - Right

P/N 662.0808A

Pump Cartridge

P/N 330.9088

S P AR E P AR T S F O R M AI N T E N AN C E & R E P AI R

F i g u r e 1 2 - 5

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 22 of 26

Waste Chamber

(Left)

P/N 662.0832

Waste Chamber

(Right)

P/N 662.0832A

W AS T E C H AM B E R S L E F T & R I G H T

F i g u r e 1 2 - 6

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 23 of 26

CONTROLLER BOARD

P/N 15-04541-01

POWER SUPPLY

P/N 330.7175

VACUUM GAUGE

P/N 662.0810

VACCUM

REGULATOR ASSY

P/N 662.1015

PERISTALTIC PUMP - LEFT

P/N 662.0808

PERISTALTIC PUMP - RIGHT

P/N 662.0808A

POWER ENTRY

MODULE WITH

FUSES

ELBOW FITTING

P/N 330.9623

PUMP CARTRIDGE

P/N 330.9088

VACUUM

REGULATOR

ASSY NOT

INCLUDED

VACUUM PUMP 115 VAC

P/N 662.0777B

VACUUM PUMP 220 VAC

P/N 662.0777C

D E T AI L S O F S O M E S P AR E P AR T S

Figure 12-7

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 24 of 26

Level Detector

WASTE PROBE (P/N 662.0788A & B)

F i g u r e 1 2 - 8

Visual LED

S P AR E C AB L E S F O R V AC U U M P U M P

F i g u r e 1 2 - 9

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 25 of 26

Always connect the tubing to the analyzer. Do not use the Vacuum

Pump System and Waste Tube with any other fluid source; direct suction of liquid from any container or cleaning of any laboratory spillages. The unauthorized use may result in serious injury to the user and permanent damage to the

Vacuum Pump System.

NO

NO

NO

NO

Always unplug the power cord from the unit before performing any installation or maintenance procedure

Remove Transit

Screws at the bottom before operating.

Do not ever leave the

Waste-Probe on the floor or outside its external Waste

Container during the analyzer operation or standby. The Waste-Probe must always be kept on the

Waste Container to avoid waste liquid spillages.

V AC U U M P U M P S Y S T E M W AR N I N G S !

F i g u r e 1 2 - 1 0

Section - XII Vacuum Pump System BT3000 PLUS Rev. 0, Soft Ver. 8, Page 26 of 26

SECTION - XIII

MEASUREMENTS & CALIBRATIONS

13-1. INTRODUCTION

The BT3000 PLUS chemistry analyzer has a general diagnostic program exclusively for the technical assistance personnel. The access to this program is protected by a password and it requires absolute familiarity with the analyzer as a prerequisite.

The following functions can be performed in this program:

1) Verification of operation and stability of the photometer.

2) Testing hydraulic functions including sampling, washing, and emptying of cuvette.

3) Verification of ISE module operation.

4) Verification and programming of the barcode.

5) Program of stress.

6) Cuvette temperature calibration (manual & automatic).

7) Reagent chamber temperature calibration (fixed or variable).

To access the diagnostic program, click on the “Analyzer” button, and then click on the

“General Diagnostic” button for displaying the “General Diagnostic” page. Now click on the desired functions.

13-1.1. PHOTOMETER CALIBRATION AND SPECIFICATIONS

The calibration consists of amplification adjustments of each channel so that these are appropriate to variables present in the photometer. The interference filters and the semireflecting mirrors may have tolerances of ±30%, which must be compensated to avoid saturation of the amplifiers.

The calibration procedure for amplifications remains the same whether it is performed with the photometer test box or directly on the analyzer. A photometer supplied by the QC with attached test report, may show different amplification values on the analyzer where it will be installed. This is due to the sum tolerances of the entire optical system: halogen lamp and quartz prism light guide. However, any slight deviation is tolerated if the values are within the specified limits. Optical conditions for calibrations and reading of values are as follows:

1) Reading station fully tested and electro-mechanically functional

2) Cuvettes calibrated and properly cleaned as per specifications

3) Distilled water, in all the 34 cuvettes (300 to 600µl)

4) Lamps tested as laid down in the specifications (see specification label)

5) Warm up period of at least 5 minutes to allow the system to reach steady state

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 10

Specifications are valid for the BT3000 PLUS with a reading cycle of 1.5 seconds and 34cuvette plate. The average value of signal for all the channels is 24,000 points

(recommended value for calibration). Limit values of signals for all the channels are from

14,000 to 30,000 points.

The reference channel has a wide excursion range of correct value from 3500 points to the 16000 points without any adjustment controls.

NOTE:

An average value of 24,000 points is recommended as the halogen lamp progressively burns out

(exhausts). After about 1,500 hours of burning the lamp may reach a minimum acceptable value limit of 14,000 points.

The acceptable minimum and maximum values have the following implications:

• Below 14,000 points the photometric resolution tends to reduce progressively to about 2,200 points of optical density.

• Beyond 30,000, towards 35,000 points, the amplifiers tend to saturate and disturb the linearity of photometer also at lower values of optical density.

13-1.2. PHOTOMETER CALIBRATION PROCEDURE

Select a pair of sequential filters (i.e. 340nm/380nm) using diagnostic program. Execute a reading of the 34-cuvette plate and observe the values of integration. If necessary, adjust the trimmer corresponding to each channel, clockwise to increase and counterclockwise to decrease the measured values. At each adjustment perform reading of the 34-cuvette plate for verification. Select the next pair of interference filters, (i.e. 405/436nm) and repeat the preceding operations. Continue this until terminating with the last pair of

630nm/700nm filters. The calibrations can also be performed in the random mode.

13-1.3. VERIFICATION OF EVENTUAL PROBLEMS DURING

THE READINGS

The verification of the photometer requires the following; selection of filters 1 and 2, the filling of one or more cuvettes, the gain reading of the amplifier of desired channel, the reading of offsets of all the channels, the reading of the absorbance in mono and bichromatic modes, zeroing the absorbance of all the cuvettes, sample one cuvette using the reagent number 1 and serums number 1 and then read its absorbance value.

1) Click on the “Select Filter” and select the 2 filters.

2) Click on the "Read zero filter" to have the transmission values in the dark of channel 1 & channel 2 amplifiers. The values should be between 0 and 390.

Values higher than that may cause problems in the photometer electronics or produce an incorrect mechanical calibration of cuvette tray (i.e.: light passage in the cuvette during reading in the dark).

3) Fill all the cuvettes with water by first clicking the "Cuvette" button and then the "Wash and fill up" button. It is possible to perform additional controls after the cuvettes have been filled.

4) It is possible to read the absorbance and the relevant values of the amplifiers on all the cuvettes by simply clicking the "Read" button. The sequence of the values has the following meaning:

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 10

Cuvette 1 st

Es.1

Chan. 2 nd

# cuv. rel. gain

18,000

chan. rel. gain

18,900 mAbs x 1 mAbs x 2 abs (filter 1) abs (F1-F2)

300 100

Selected Filters: 340nm/630nm

The above example shows that the cuvette #1 (with H

2

O) has an absorbance of 300 mABS at 340nm and an absorbance of 100 mABS in bi-chromatic reading at (340-

630nm). The relative gains of the channels #1 and #2 should have the values between

14,000 and 28,000 when all the cuvettes are filled up with distilled water.

If the zeroing of the absorbance is desired then click on "Zeroing" button, if necessary click a couple of times. When performing the a sample and read process using reagent in position #1 and sample in position #1, click on the "Sampling" button and select the appropriate method "Normal Sample" or "Sample Starter".

In the "Normal Sample" mode a sequential sampling is carried out with the same needle, first reagent and then serum. The volume of the reagent is fixed at 300µl, while the volume of the sample is selectable from 0 to 27ml. Take into consideration that the instrument restores the stoicometric ratio when the volume of the sample has been selected. If 3µl of serum is chosen, then the reagent volume becomes 297µl. After the sampling, it is possible to read the absorbance values of the sample directly in the mono or bi-chromatic mode using previously mentioned commands. The "Sample Starter" mode is similar to the preceding method except the sample, which is dispensed subsequently by the second sampling arm. The sampling test is always carried out in cuvette #1.

13-2. CUVETTES CALIBRATION AND TEMP. CONTROL SPECS

The calibration involves adjustment of the temperature sensor to manufactures specifications. A LM35 chip, with tolerance of ±1°C, is used as the sensing element and is located inside a metallic housing.

Operative conditions for correct temperature calibration inside the cuvettes are as follows:

• Ambient Temperature: 18°C to 32°C

• Analyzer turned on for at least 20 minutes to reach steady state

• All 34 cuvettes filled with 300µl to 600µl volume of distilled water

• A certified electronic thermometer with micro-probe with at least 0.1°C resolution

Keep in mind that a thermometer probe of 2 to 3 mm diameters, equipped with a K type thermocouple, when immersed in the cuvette, produces an instantaneous temperature drop of about 0.2°C.

We advise against the use of thermometers of medium to large dimensions to avoid incorrect measurements because of difficulty of inserting the probe and for the necessity of performing measurements quickly. Do not perform measurements or calibrations if the cuvettes have been filled with distilled water without waiting for necessaryl 20 minutes warm-up period

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 10

13-2.1. CUVETTE TEMP. CALIBRATION PROCEDURE

Prior to proceeding with calibrations, set the cuvette temperature at 37ºC in "ANALYZER

SETUP" page.

Manual temperature calibration

The manual temperature calibration adjustments are made through trimmer located on the "MASTER LOGIC BOARD" (on the rear lower part of the reading station).

Automatic temperature calibration is possible in the software version 7.2 onwards, refer to instructions.

Enter the service page and select the icon "Check Temperature". The cuvettes plate stops temporarily. Immerse the temperature measurement probe in an easily accessible cuvette. Gently shake the probe with rotational movement (for mixing) at the bottom of the cuvette to quicken the measurement.

After measuring and noting the correct and stable value, stop the operation. Do not exceed 45 seconds of measurement time to avoid that irregular heating underneath affect the temperature of the rotating plate. The temperature monitoring system functions correctly, only when the metallic mass of the cuvettes rotor makes cyclical motion. After making a correct measurement, note down the measurement value, remove probe from the cuvette and click on the button, which appeared previously "Push Button...". The plate starts rotating clockwise and counterclockwise in the alternative manner, instantly the temperature is displayed in real time just below the commands line. The displayed temperature value should be identical with previous value measured with the probe.

If necessary, adjust the TR1 trimmer on the Master Logic Board (15-04211-01) to match the value measured by the thermometer probe. Make this adjustment in the shortest time possible. The value of temperature measurement should not be necessarily 37°C as it is automatically corrected when exiting the service page. For example, the measurement of cuvette temperature by thermometer in a motionless plate is equal to 35.9°C. The measurement displayed on the monitor in the "Check Temp" function with plate rotating alternatively between clockwise and counter clockwise directions is 36.5°C. Adjust the trimmer TR1 until the temperature of 35.9°C is displayed on the monitor. Exit service page of "Check Temp".

After the settling time of a few minutes, the monitor should display the correct temperature with fluctuation of ±0.2°C.

Automatic temperature calibration, only with software 7.2 onwards.

An appropriate thermometer is necessary (as indicated previously) to perform a valid calibration. With the instrument in steady state condition and the cuvettes filled with water, enter the diagnostic page (Analyzer > Diagnostic > General Diagnostic >

Mechanics). Select "Mechanics" and click "SET TEMPERATURE".

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 10

Select "Temperature Calibration" in the next window. This is a step by step guided procedure. Insert the certified thermometer probe tip into the cuvette and after 45 seconds read the measurement value. Write the exact temperature measurement value

(including decimals) by using mouse and the keyboard. After this operation is terminated the instrument will assume the set value after couple of minutes.

It is possible to view on the display the measurement value of temperature in the cuvette.

Some times one can observe the temperature fluctuations of +/-0,2°C, caused by the automatic corrections of feedback circuit. However the real variations in the cuvette are much less because of the considerable mass of the cuvette system components.

NOTE:

Verify the following parameters for an accurate calibration:

1)

2)

3)

4)

The water must be poured into the cuvette at least 10 minutes before the calibration.

The ideal ambient temperature should be between 20ºC and 26ºC.

The precision thermometer probe must be of miniature size. The instruments for measuring temperature such as thermometer having glass tube with mercury or alcohol are not suitable for this purpose.

The trimmer used in manual calibration adjustments is not required in the automatic calibrations. Therefore this trimmer must not be adjusted during or after the automatic calibration.

13-3. REFRIGERATION SYSTEM CALIBRATION AND SPECS

The thermostatic control system of the reagent chamber consists of a refrigeration system based upon Peltier modules, which are affixed underneath the circular aluminum chamber. This system maintains reagents at low temperature. The system has refrigeration limits when the ambient temperature exceeds 28ºC, consequently the reagent chamber temperature increases beyond the preset temperature. The electronics of the temperature regulation is inside the "REAGENT CONTROLLER" board.

A reagent controller board was produced up to July 2004, which had fixed regulation of temperature. The controller used an approximate value of 5ºC as reference. After 15th

July 2004, a new controller board was introduced in the production, giving the user a facility to select temperature between 5ºC and 15ºC.

13-3.1. REAGENT CONTROLLER P/N.15-04313-03 (OBSOLETE)

Unadjustable temperature at 5°C (installed prior to July 2004).

The control is realized by varying the Peltier supply voltage from 16 to 28 volt approximately through a feedback system between the temperature sensor LM35

(located under the aluminum chamber) and the 400 Watt power supply. The comparator

(chip U6B) changes the state, when by system fault a temperature exceeding 43ºC is read. In this case the switch (MOSFET Q2) interrupts voltage to the Peltier modules. If one desires to interrupt the refrigeration through Setup Analyzer then the same MOSFET disconnects the system through software.

When the ambient conditions exceed the extreme operating conditions (18ºC and 32ºC) the system limits are evident. Particularly, the ice formation in the reagent chamber is possible below 18ºC and beyond 28ºC the preset temperature value of 5ºC starts rising.

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 10

13-3.2. REAGENT CONTROLLER BOARD P/N 15-05211-01

Temp. adjustable from 5°C to 15°C. (installed from July 2004 onwards).

This electronic circuit as compared to the previous circuit allows the refrigeration chamber to reach lower temperatures (below 10ºC). In addition the user can select the desired operating temperature in the setup. The real value of the selected temperature will be displayed on the analyzer display.

The temperature sensor is read by the µC U3 767, which in turn proportionally regulates the voltage of the 400W power supply. Because of the limited excursion of the regulation under a certain temperature value, the µC 767 interrupts the operation of the cross blower fan to avoid excessive decrease in temperature. The final regulation is optimized between 8°C to 28°C (+/- 1,5°C) of ambient. When the room temperature exceeds the

40ºC, this circuit also interrupts the system for defect. A supplementary safety circuit consisting of a NTC (NTC1), located near the power connector J2, completely cuts off voltage to the Peltiers. The purpose of the circuit is to detect temperatures higher than

75ºC on the printed circuit, possibly caused by the false contacts in the connections. This circuit also functions with the analyzer in the sleep mode, thus assuring the selected temperature.

13-4. BARCODE CALIBRATION AND SPECIFICATIONS

The calibration involves the aligning of the barcode scanner beam in the precise direction corresponding to the reagent bottle and the sample tube in the serum plate. The mechanical adjustment should be performed with the aid of the diagnostic program. The barcode module should also be programmed for the special reading of up to five types of barcode labels.

13-4.1. PROGRAMMING OF SERUM/REAGENT BARCODE

Click on "Mechanics" button to access the barcode diagnostics program. The available functions allow the user to verify and optically align the scanner beam on the reagent and sample barcode labels. It is also possible to read the information of the barcode label if it is present.

The mechanical alignment can be carried out by repeatedly clicking on the desired sample or reagent barcode button. It is possible to precisely align the scanner beam on the barcode label after loosening the mounting screws. The barcode controller can also be programmed in the diagnostics program by using a specific floppy disk available upon request.

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 10

13-4.2. BARCODE - MECHANICAL CALIBRATION

In the same "Mechanics" page, select the desired barcode scanner to be calibrated mechanically. Click on the command button "Laser Scan". Verify that the scanner beam is precisely centered on the barcode label of the reagent bottle or the sample tube in the serum plate. It is possible to correctly align the barcode modules by loosening the mounting screws. After the alignment, fasten the screws. Refer to Paragraph 13-6. entitled "Primary Tube Sensor Calibration and Specifications" for the final verification of the correct operation of the barcode module.

13-5. ISE MODULE CALIBRATION AND SPECIFICATIONS

The calibration involves the adjustment of sensibility threshold of bubble sensor and the verification of correct operation of the hydraulic circuit and electronics.

The bubble sensor calibration should be performed by measuring the voltage of the electronic photo-switch with an empty and a full fluid tube inside. The difference between the two measurement values must be of at least 0.5 Volts.

The diagnostic program for the I.S.E module, allows verification of the drift of the electrodes, the efficiency of the hydraulic circuit and the bubble sensor. It does not allow the calculation of slope and therefore the real efficiency of the electrodes.

The commands are as follows: "ISE Module Prime", "Read ISE", "Fill Up ISE", "Empty

Out ISE", and "Read Sensors".

The first command "ISE Module Prime" is essential for setting bubble sensor sensitivity threshold, when accessing the diagnostic program for the first time. The command, “Read

ISE” reads potentials of all the electrodes in mV. The potential of the REF electrode is displayed separately in the upper section of the screen. The clicking of the “Fill up ISE” button performs a baseline sampling followed by a reading. Reading repeatedly with

“Read ISE” it is possible to observe the potential and therefore the drift.

The expected values should be between +500mV and -500mV. The REF should have a value between 2.2 Volt to 2.9 Volt. The values other than the expected values may indicate an improper flow in the electrodes or in the worst case a defective electronic circuit. A typical example of electrodes malfunction is encountered when handling the electrodes during the maintenance. One can inadvertently touch the gold-coated contact pin of the electrode, causing an electrostatic charge in the electrode. In this case, it is possible to observe the continuously changing values of the potential.

To verify the efficiency of the bubble sensor, click on "Read Sensors" button to display the fluid tube "Full Value" and "Empty Value" in volts. The difference between the two values should be higher than 0.5 Volts for the correct operation of the sensor. A difference of less than 0.5 Volt indicates that the optical sensor has not detected sufficient variation between air and liquid. This may be due to fluid being stationary in the segment of tube across bubble sensor or the tube has lost its original transparency.

Finally, the command "Empty ISE" is a useful option for emptying the ISE mixing funnel.

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 10

13-5.1. ISE BUBBLE SENSOR CALIBRATION PROCEDURE

To calibrate the ISE bubble sensor, proceed with the following: a) Click "Analyzer". b) Click "Diagnostic". c) Click "General Diagnostic" d) Click "ISE MODULE". Place a bottle containing Baseline on the small tray in the ISE module and click "ISE Module Prime" and wait for the termination of prime cycle. e) Click "Read Sensor", and wait for the values (Full Value and Empty Value) to appear automatically on the screen. The difference between the two values should be more than

0.5 Volt.

NOTE:

The measurement value generated by sensor for tube filled with Baseline (Full Value), must be between 3 Volts (minimum) and 4.2 Volts maximum. If otherwise, then lift out the ISE module from its track rail (even with the analyzer turned on) and turn the TR1 trimmer adjustment screw clockwise to increase or counter clockwise to decrease the voltage. See

Figure 1.

THE TRIMMER TR1 IS

LOCATED ON THE REAR

OF ISE MODULE, JUST

BEHIND THE BUBBLE

SENSOR

ISE MODULE

Figure 1

f) Put back the module in its place and press the key F5 to perform a general reset. g) Repeat the preceding steps (for tube filled with liquid) until the desired values are obtained.

NOTE:

In case the delta results are below the threshold of 0.5 Volts, this may be due to incorrect observation of calibration procedure, or malfunction of hydraulic circuit or photo-switch.

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 10

13-5.2. ISE HYDRAULIC CIRCUIT VERIFICATION

It is very important to control the flow of liquids in the hydraulic circuit. During the prime function, the Baseline must completely fill up the Reference electrode, thus keeping the hydraulic circuit full and without air bubbles - starting from the "Y"-connector to the ground electrode.

CAUTION!

Any interruption in the flow of liquid causes air bubbles and affects measurement of potentials of all the electrodes.

Enter the "Utility" page and click on the icon "Prime ISE". After the prime function terminates, gently lift out the ISE module and observe if the hydraulic circuit is full. Put back in place the ISE module and press F5 to reset.

13-5.3. ISE ELECTRODES POTENTIAL VERIFICATION

It is possible to make a precise measurement of the electrodes potential through

Diagnostic program.

IMPORTANT NOTICE: Prior to verification, the ISE system must be primed.

After entering the diagnostic page concerning the ISE, click "Fill Up". In this operation the

Reference Solution fills up all the electrodes including the reference electrode. After the sampling read the potentials by clicking on the "Read ISE". After some seconds the monitor displays the mV measurement values of 4 selected electrodes. The electrode potential is expressed in Volts and is displayed on the upper part of the screen.

The expected values for Ref, Na, K, Cl and CO

2

electrodes should be within the range -

400mV to +400mV. If the values do not correspond then there is a probability that a hydraulic circuit problem did not let the solution correctly fill up the electrodes. Note that, if the electrodes are empty, they can assume any potential value. It is also possible that only the REF electrode is empty. In this case, it will affect the level of the other electrodes. Verify the correct operation of peristaltic pump for emptying and the pinchvalve tubing.

Other situations that may cause a malfunction of ISE module are as follows:

1) One or more electrodes out of range because they were subjected to electrostatic discharge during handling

2) Defective preamplifier

3) Incorrect reference solution

NOTE: The reading of potential does not indicate any exhaustion or expiration of an electrode. The electrode's efficiency is verified only through the SLOPE performed by a calibration and the performance characteristics listed in the package insert.

For additional details refer to ISE troubleshooting.

13-6. PRIMARY TUBE SENSOR CALIBRATION AND SPECS

The calibration consists of sensitivity threshold adjustment of electro-optical system (LED and photodiode). The optical element exploits the principle of reflection. The infrared light beam emitted by LED is reflected back from the primary tube and received by the photodiode sensor element. One can proceed with calibration after placing three 10mm diameter primary tubes with appropriate adapter in a triangular configuration (i.e.: in positions 02, 03, 29 on the serum plate.)

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 10

There are two primary tube sensors. The one on the left of serum chamber detects the presence or absence of primary tubes appropriate to the left sampling arm. The one located on the right of serum chamber detects the presence or absence of tubes pertaining to the right sampling arm used for aspiration of serum for ISE, starter and dilution. The sensitivity threshold should be adjusted in such a way that the primary tube sensor detects only the primary tube and not the interference from the adjacent tubes.

A green LED located on top of the detecting circuit provide the user with a visual indication of the sensitivity threshold monitoring for distinguishing the primary tube. The removal of front casing is necessary for making any adjustment or service.

As a first step use unlabelled 10mm tubes for calibrating in worst conditions. Manually rotate the serum plate with slow motion so that the three primary tubes #02, 03, and 29 sequentially move past the primary tube sensor. Bear in mind that the waste funnel is the reference point of the sensor located on the same axis. Observe that with each primary tube moving past the primary tube sensor turns on and off the green LED.

A sensor with an excessive sensibility does not distinguish the spacing between the primary tubes and on the contrary it may not detect the presence of a tube particularly the one located on the inner circle further away.

The sensitivity is adjusted through trimmers TR1 located on the boards 15-04280-01 and

15-04278-01. Turning the trimmer adjusting screw clockwise increases the sensitivity and turning it counterclockwise decreases the sensitivity. Keep in mind that the analyzer must always function with labeled tubes therefore it is preferable to adjust calibration to lower sensitivity rather than to higher sensitivity.

The final test is performed by fully scanning the serum plate and by inserting at least six tubes with barcode labels. Placing three tubes in triangle formation in positions #02, 03, and 29. Now place three tubes in positions #15, 16, and 42. Once completed, go to the

"Patient Entry" page and enter "Options" to start "Scan Tray-All". At the end of scanning, if the primary tube sensor has correctly tracked the presence of all the six tubes, then their barcode numbers should be visible in the patient page. This means that the primary tube sensor and the barcode scanner have functioned properly.

If scanning problems occur, the following two types of error messages may appear: a) "Label Not Found in Pos.28": all primary tubes have been detected. The barcode scanner has not read the barcode label correctly because it is defective. b) Barcode numbers of 5 primary tubes displayed, instead of 6 tubes. The primary sensor has not detected the remaining one primary tube. Verify the mechanical calibration of the serum plate and ultimately the sensitivity of the optical detection circuit.

13-7. STRESS ANALYZER

This program performs a limitless cycle of mechanical stress on the analyzer. Generally this program is used for verifying the efficiency of replaced mechanical devices after service and repair.

Section - XIII Measurements and Calibrations BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 10

SECTION - XIV

TROUBLE - SHOOTING

14-1. INTRODUCTION

Any investigation of malfunction, which might occur, must begin with verification of power supply and the ambient conditions. During troubleshooting the following parameters for correct analyzer operation must be verified:

Power Supply: 100 – 240~, 50/60 Hz

Ambient Temperature:

Relative Humidity:

18°-32°C

10 - 85% non-condensed

In case the parameters do not correspond to the indicated range of values, then observe the following procedures and the appropriate corrective actions. Prior to servicing the BT3000 PLUS chemistry analyzer, switch off the instrument and disconnect the power cordset.

14-1.1. TROUBLE-SHOOTING CUVETTE TEMPERATURE

SYMPTOMS

CUVETTE TEMPERATURE

CAUSES AND CORRECTIVE ACTIONS

Cuvettes do not reach operating

1) Ambient temperature out of limits. Reenter ambient temperature limits 18°- 32°C.

30°, 32° and 37°C for temperature to exceed maximum limits. Replace the or unstable temperature sensor or the master power board or the chip temperature. µC764. Refer to "Measurement and Calibration" procedures for the reading station outlined in the Section XIII to find out the defective device. After replacing the temperature sensor, there may be temperature fluctuation of ± 0.5°C. In this case make adjustments as per calibration procedure.

3) A defective Peltier module will cause temperatures to be lower or near ambient temperature. Check if there are one or more defective Peltiers. Even one defective Peltier can stop the temperature monitoring system.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 15

14-1.2. TROUBLE-SHOOTING REAGENT CHAMBER TEMP.

SYMPTOMS

REAGENTS CHAMBER TEMPERATURE

CAUSES AND CORRECTIVE ACTIONS

The base of the reagent chamber does not reach

1) Defective Cross-flow blower causing high temperature. Verify and replace if necessary. temperatures (5°-20°C) or some times seems to be heating. In some cases excessive cooling with ice formation.

Verify and replace defective Peltier.

3) Defective temperature sensor or reagent control board causing overheat or ice formation. Verify and replace the defective part.

14-1.3. TROUBLE-SHOOTING POWER SUPPLY

SYMPTOMS

POWER SUPPLY PROBLEMS

CAUSES AND CORRECTIVE ACTIONS

1) The analyzer turns off suddenly without any apparent reason. The

1) Power supply problems: defective main power, power cord not properly connected, UPS connected incorrectly. Get your main power line checked and vacuum pump system makes unusual noises. repaired by a competent electrician. Connect power cord correctly.

2) UPS (if installed) generates continuous

2) Main power line generating high surge voltage due to lack of ground connection. Request your electric alarms. company to verify and repair the line if necessary. generates random resets of mechanical devices or the software.

UPS temporarily to verify if it is causing the problem

If necessary substitute with new UPS. Try to recharge the batteries or substitute them. Bear in mind that the UPS supplied with the instrument is universal type and it cannot be used in extremely critical situations where the electrical lines are subject to large surges. In such cases it is recommended to use a more sophisticated UPS (i.e.

NOBREAK, etc.)

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 15

14-1.4. TROUBLE-SHOOTING POWER UP PROBLEMS

SYMPTOMS

POWER UP PROBLEMS

CAUSES AND CORRECTIVE ACTIONS

After turning on the 1) No power due to various causes. The monitor is off; analyzer, nothing happens. only the refrigerator fan can be heard. Verify all the connections and the correct operation of the UPS.

2) The computer has not been turned on through its push button. Press push button to turn on the computer.

3) Hardware problem: defective motherboard or power supply. After verifying, repair or replace.

After turning on, the 1) The analyzer has been turned off incorrectly through program loading main switch or sudden main power line interruption

(bootstrap) by the with consequent damaging of some files. Wait for the computer is interrupted and the computer blocks. Turning off the computer with the push disks verification to be performed automatically by the system. Verify any errors in the hard disk.

Contact the service engineer for any problems.

2) Hardware conflict between the system peripheral button becomes devices or damaged peripheral device. The necessary. peripheral device must be reconfigured or substituted in case of damage. Contact service engineer.

14-1.5. TROUBLE-SHOOTING THE MOUSE AND KEYBOARD

SYMPTOMS

THE MOUSE & KEYBOARD

CAUSES AND CORRECTIVE ACTIONS

The system performs the boot correctly, the analyzer logo appears and seems to be ready, but the cordless keyboard and mouse do not work.

1) The RF Receiver cable disconnected from the rear panel. Check and correctly connect the cable.

2) Exhausted batteries in the keyboard or the mouse.

Substitute the batteries with the analyzer power on; press the devices button to put them in communication (setup). The sequence is: RF

Receiver, keyboard and the mouse.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 15

14-1.6. TROUBLE-SHOOTING RESET ERRORS

Reset Errors: Serum Plate/Reagent Tray/Diluter/Arm

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

The analyzer after 1) Inadvertent accidental blockage by operator of one routine boot displays an or more modules. For correct operation perform a error message about one of the following modules:

• Reset error of serum plate

• Reset error of reagent tray

• Reset error of diluter

• Reset error sampling arm etc. general reset (F5 or Reset Icon).

2) If the problem persists the problem may lie in the mechanics or electronics of one of the modules. The defective module seems to be operating but an error message appears.

NOTE: To diagnose a mechanical or electronic problem is quite simple. Generally speaking a mechanical defect produces a partial movement in the device and careful observation of the mechanism during operation one can easily identify the problem. The electronic problems are related to motor drivers and the position sensors. In case the device is functional but does not stop in correct position, the problem in the reset sensor. Depending upon the module it may be a photo-switch or Hall sensor.

These specified problems might associate to diluter, reagent tray, serum plate, cuvettes plate, and the washing piston. If the device makes no movement then there is a defective circuit of the power driver or a defective motor. Consideration should be given to the fact that all modules have the same basic structure: motor, driver, position sensor and the micro-controller. The service operations for resolving the problem are deliberately confined to the substitution of the defective devices already mentioned. In case the service engineer is unable to resolve the problem, the whole electronic circuit of the module can be substituted. It is important to verify that the devices are properly connected and that the cables and connectors are free of defects.

It is also important to try to distinguish the hydraulic problems from those of electronics as regards analyzer malfunctions. Any improper evaluation of problems may lead to inadmissible loss of time. Outlined below are

some procedures for quickly locating the problems.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 15

Reset Errors: Serum Plate/Reagent Tray/Diluter/Arm

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

Diluter blocked:

DILUTER

Defective motor, driver, micro-controller, connecting cable, or PCB. Repair or replace.

Diluter functions but does not reset:

Defective photo-switch, micro-controller, connecting cables, or PCB. Repair or replace. In case the Diluter is damaged or defective then it should be completely substituted.

Sampling arm moves

SAMPLING ARM

Defective Hall sensor or positioned incorrectly. Repair or vertically without resetting:

Replace

Sampling Arm blocked: Defective driver or interrupted motor. Bear in mind that the arm does not make angular (swivel) motion until after the vertical reset. To verify the correct functioning of the three arm sensors (Hall sensor for UP/DOWN motion,

Liquid Sensor, Photo-switch for angular motion) observe the three corresponding LEDs located on a small PVC bracket near the arm shaft. If the arm is moved up/down manually, the upper LED lights to indicate the correct functioning of the Hall sensor. The touching with a finger of the arm needle will light the middle LED. Swiveling the arm to the left or right will light and switch off the lower

LED. Repair or replace defective motor or driver.

Zero position calibration of the sampling arm not

The upper part of the arm (needle head) has loose screws and is not secured to shaft properly. Dirt in the maintained in successive resets:

Tighten the screws. Clean with a brush the photo-switch or the shutter.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 15

Reset Errors: Serum Plate/Reagent Tray/Diluter/Arm

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

The arm needle holder does not heat up:

Interrupted heater circuit inside the sampling arm, in this case the liquid sensor functions as the LED lights up just by the bare touch of the finger. On the other hand if the

LED does not light up then the electrical circuit is interrupted at the source. Verify the power supply starting from the source, the Master Power Board 15-04209-01. If the arm is damaged or defective then it is recommended to substitute it completely.

The serum plate rotates during reset and stops at an incorrect position with a screen message

"Reset Error...":

SERUM PLATE

Most likely the Hall sensor malfunction. The magnet on the upper rim of the serum plate is not properly aligned with the Hall sensor. Defective Hall sensor or connecting circuit interrupted. No rubber mat over the rotating serum base. In the absence of rubber mat the magnet position is displaces a few millimeters downwards thus causing misalignment with the Hall sensor. Other various causes:

Magnet detached from the plate or defective electronic circuit (Board 15-04282-01 & Board 15-04422-01). The serum plate resets correctly but does not respect the positions and the sampling needle descends in the wrong positions. Most likely the serum plate slips on the rubber mat during rotation. Remove and wash the rubber mat with a neutral soap and water. If the problem persists then the causes may be as follows: Transmission problem with one of the following devices: Timing Belt, motor, driver and Control Board (15-04422-01).

REAGENT TRAY

The tray does not stop 1) Defective Hall sensor or the sensor out of range from at the container number magnet. Repair or replace.

1 position and issues an error message

"Reset Error...":

2) Defective belt drive, stepping motor, driver, or

Control Board 15-04313-03. Repair or replace.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 15

14-2. COMPUTER MODULE TROUBLE-SHOOTING

The diagnosis of computer system malfunction should be quite simple, as the problem generally occurs during the powering up of the analyzer. The most frequent problems are generally the loss of files or a hard disk malfunction.

Statistically these problems almost always are caused by incorrect operations as follows: Turning off the analyzer incorrectly, using back-up disks that is infected with a virus, incorrect use of the computer, etc.

14-2.1. TROUBLE-SHOOTING THE COMPUTER MODULE

COMPUTER MODULE TROUBLE SHOOTING

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

The analyzer starts up but no LCD display.

1. Probably a defective VGA driver or incorrect connection of the LCD display. Check on the rear of display that the lamp is lit. Check that the VGA board is properly inserted into the slot of mainboard. Make sure that all the cables of display including the inverter cables are properly connected. If necessary replace

VGA Board.

2. The inverter of the display lamp power supply defective or incorrectly connected. Check that the inverter cable is correctly connected. If necessary replace inverter.

NOTE: It is also possible to connect an external CRT monitor to identify the problem. functions now and then only in some zones of the screen. serial port where the driver is connected.

Gently clean the LCD display screen with a soft lint free cloth moistened with an appropriate neutral detergent and delicately wipe to dry up. Check that the flat cable of touch-screen is properly connected to the driver. If necessary replace driver or serial port.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 15

COMPUTER MODULE TROUBLE SHOOTING

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

After loading Windows, the loading of the

1. No communication between the computer and the process electronics. Check that the flat cable across process program starts and is visible on the

IBM and the Main Logic Board (on the rear of the reading station) is properly connected. progression bar.

The progression bar

2. Defective RS-232 serial communication port of the computer or the micro-controller. Check and replace if inactive, followed by an necessary.

3. Defective Main Logic Board. Verify and replace if

"Impossible to Reset necessary.

Analyzer". All electromechanical devices remain motionless. between the analyzer cable. Verify and replace serial port. Check that the and the host computer. interface cable is correctly connected.

NOTE: If the interface with the host computer is done for the first time then there may be a software problem. To avoid software problems, use specific software protocols for verification of communications.

14-2.2. TROUBLE-SHOOTING THE BOOTSTRAP

BOOTSTRAP - COMPUTER MODULE TROUBLE SHOOTING

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

After turning on the There is a floppy disk in the floppy disk drive of the computer the bootstrap computer. Remove floppy disk and press any key. process interrupts and displays an error message: “NTLDR not found press any key to restart".

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 15

BOOTSTRAP - COMPUTER MODULE TROUBLE SHOOTING

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

During the bootstrap 1. Hard disk cable disconnected. Connect the hard disk process, an error cable properly. message appears on the screen: “Disk boot

2. failure, insert System 3.

Disk and press Enter"

Damaged or unformatted hard disk. If necessary, replace the hard disk. Format the hard disk.

Incorrect configuration in BIOS setup. Verify the

STANDARD CMOS SETUP and particularly the configuration of the Hard Disk.

Note: Guidelines for installation of the operative system or the application programs on the BT3000 PLUS (complete reinstallation and/or substitution of hard disk and/or replacement of motherboard) is required for motherboards of new generation.

4. Configure the startup sequence in BIOS setup with CD-

ROM drive as the first peripheral, the hard disk as second peripheral and the floppy disk drive as the third peripheral.

Caution! After installation of the Windows ® 2000 PRO operative system, restore the original BOOT configuration.

5. Install Windows 2000 PRO by starting the computer with the CD and carefully select FAT32 as file system.

Insert the user name, organization name and the password: <<administrator enzo>>.

6. Enter Control Panel - System - Hardware and then

Device Manager to verify the correct installation of all the devices. Eventually remedy the causes of any conflicts and if necessary, install an appropriate updated driver for any unidentified device (Audio, additional serial port, and touch-screen etc.).

7. Install application software of the external peripherals

(i.e. printer, UPS, etc.)

8. Install application software of BT 3000 PLUS.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 15

BOOTSTRAP - COMPUTER MODULE TROUBLE SHOOTING

SYMPTOMS CAUSES AND CORRECTIVE ACTIONS

After power on and Enter Control Panel - Users and Passwords – remove the during the bootstrap "X" mark from the box corresponding to the <<User must appears on the display. during reinstallation of hardware devices (i.e. additional serial boards, touch-screen etc.) by another manufacturer was installed and is still present in the system even if physically disconnected.

Note: In the case of serial board, two new gates COM3 and COM4 are assigned to replace COM5 and COM6. In such a case it is necessary to enter the Advanced Settings of the appropriate serial communication port and modify it manually.

14-3. UNRELIABLE RESULTS OF ANALYSES

General Considerations:

Any errors by the operator in application of method, use of appropriate reagent, or a sample of unknown origin are excluded a priori.

14-4. METHOD FOR VERIFICATION OF PHOTOMETRIC

INSTABILITY

It is possible to make quick verification of photometric stability by using the general diagnostic program, excluding sampling problems.

Fill up (in "Photometer" mode) all the cuvettes with H

2

O through appropriate command. In case of hydraulic defects, manually fill the cuvettes. Select a pair of interference filters, where 700nm filter is always selected as the second filter. Perform a zeroing and then the consecutive readings, while observing the stability of absorbances of all the cuvettes (last column on the right). Normally the values should be around +/- 1 point. Repeat a few times after short interval of some minute to insure long-term stability of readings.

If considerable variations are observed in a short period of time, then instability in the photometric lamp is to blame. If the instability regards one or more cuvettes, then it is likely that there are foreign debris or moving corpuscles inside. In this case remove the foreign debris or corpuscles by using the aspiration tube of the washing piston plunger

(located on the upper end of the piston plunger).

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 15

To verify the photometric instability (relevant only to the photometer), execute the command "Reading" followed by "read zero filter". The offset values of all the channels will be displayed. The values should be stable and within the range of 0 to 150 points. If otherwise then one should suspect the infiltration of water in the photometer caused by a defect in the hydraulic circuit. In this case one should assume that the water might also have seeped into the cuvette rotor. In this case it will be necessary to completely remove the cuvette plate and rotor, and then thoroughly dry the whole system including the cuvettes.

14-5. VERIFICATION OF HYDRAULIC SYSTEM OPERATION

The hydraulic circuit consists of the diluter, peristaltic pumps, and pinch valves. The instrument detects any malfunction of the preceding devices during power on.

Appropriate alarms indicate the type of problem: dilutor reset error, collapsed or sticking tube in pinch-valve, water lacking etc. The messages are generated during test runs for lack of water and the dilutor reset error.

The poor reproduction of results is generally caused by an imprecise aspiration of sample or reagent, insufficient vacuum level for the reading cuvette washing and drying. In the first case verify the correct operation of the dilutor, correct alternating motion of the piston and the absence of air bubble in the exposed hydraulic circuit. As regards the cuvette washing and drying phase, verify the water consumption necessary for the washing of 34 cuvettes. To verify the water consumption place the water intake tube (from the external water supply container) in a graduated beaker or cylinder filled with at least 500 ml of distilled water and launch the function "Wash Cuvette". At the end one should observe a water consumption of at least 150 ml, which is equivalent to 4,5 ml per cuvette. Any lack in amount of water consumption may depend on scarce vacuum level or a partial occlusion in the washing piston.

A frequently neglected additional cause of photometric instability is due to the poor overall maintenance of the analyzer. Particularly the irregular routine washing of the cuvettes may cause a progressive contamination the cuvettes through deposits, which are difficult to eliminate.

Just consider a gradual stratification of reagents on the inner surfaces of the reading cuvette, their gradual release during tests and the possible consequences. In such cases it is of utmost importance to perform a thorough washing by using hydrochloric acid 0,1N

(instead of routine washing solution) and leaving it inside the cuvette for at least 5 minutes. Then follow up with a standard H

2

O wash. After having given consideration to the preceding circumstances, an error may emerge during the photometric zeroing phase caused by variable conditions of transparencies of cuvettes during the washing progression. Therefore perform a "SETLAMP" to restore the reference conditions and then a "Zeroing " or "Photometer zeroing".

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 11 of 15

If the routine maintenance is performed regularly, the ISE module functions correctly. The most common problems encountered in the ISE Module are as follows: b) c)

Unreliable sample results with flags.

Error messages of air bubbles. a) CALIBRATION ERRORS

The causes may be due to various reasons and can be diagnosed through close examination of mV values of slopes.

Typical values of calibration potentials in mV:

K std low = -8 mV

Cl std high = +22 mV difference 30 mV (slope 57 mV)

Na std low = -10 mV difference 15mV (slope 63 mV) std high = + 5 mV std low = std high =

+6 mV

-4 mV difference 10mV (slope 50 mV)

CO2 std low = +4 mV difference 10mV (slope 32mV) std high = -6 mV

The above-mentioned values are indicative and may deviate due to the wear and tear of the electrodes.

A positional error or an incorrect concentration of reagents may determine a considerable variation of potentials and slopes.

The mV values (in brackets) in the calibrations printout can give additional indications, which are useful for the diagnostics.

For example:

Std low -8mV (-208 -200)

Std high +22mV (-178 -200)

One can observe in the above example the Baseline value (-200), which must be always included between two Std values. In fact the value -200 is between the two values -208 and -178.

Various combinations of error a) Inversion of Buffer / Baseline

Considerable increase of potentials (up to 100mV), decrement of slopes. b) Insertion of identical reagents: Buffer / Buffer or Baseline / Baseline.

The potentials are almost within norms but there is considerable decrement of slopes.

In the above items a) and b), the Baseline values shown in the preceding example shall not be coherent.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 12 of 15

b) OBSTRUCTIONS CAUSED BY PROTEINS IN ELECTRODES CL &CO

2

After approximately 150 to 200 samples, the Cl & CO

2

electrodes may be periodically obstructed. In this case, one observes an increase in the slopes of K & Na, and decrement in the slopes of Cl & CO

2

. Follow the instructions for the cleaning of electrodes.

A partially occluded Ground electrode may also cause a similar error.

Generally, the obstructions are caused by the foreign matter and not by protein.

Carefully observe the cleaning procedures as outlined in the Figures 13 and 14 on the ensuing pages.

NOTE:

A prolonged obstruction of the Cl electrode may progressively obstruct the electrodes below it (CO

2

and

Bypass). For cleaning these electrodes, use syringe for the CO

2

and the cleaning tool on the Bypass as shown in the Figure 14. c) BAD RESULTS OF SAMPLES:

Generally, this phenomenon occurs during sampling but it may take place afterwards. Observe the above-mentioned diagnostic procedures. d) ERROR MESSAGES, AIR BUBBLES, FLAGS

During a calibration or a run an error message "Air bubbles" may appear. The error may be momentary and not appear afterwards. In this case, it means a casual formation of bubbles. If it persists then it may be due to problems of hydraulic nature. a) ISE funnel takes long time to empty. It may be caused by defective or worn peristaltic pump for draining or an obstruction in the electrodes stack. Repair or replace. b) Prime operation failure. The Prime also calibrates the bubble sensor. Check that prime is performed properly. c) Collapsed tube in the ISE pinch-valve. The Baseline solution doesn't enter the Reference electrode and the funnel doesn't empty. The Reference electrode is filled during the prime. Check and correct. e) RESULTS WITH FLAG "D"

During the reading, the flag "D" on one or more electrodes indicates a drift of potential greater than 2 mV. The phenomenon may be determined by one of the following causes:

••••

Defective electrode.

••••

Electrode to be reconditioned.

••••

Partial occlusion of the electrode.

••••

Incorrect reagents.

••••

No washing after a consistent run.

••••

Correlation with general problems already explained.

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 13 of 15

NOTICE

C H L O R I D E E L E C T R O D E

NEEDLE TOOL P/N 03254

It is often possible to extend the life of a chloride electrode by using the supplied

CHLORIDE ELECTRODE NEEDLE TOOL.

1) Grasp the NEEDLE TOOL as shown. Gently insert the tip of the needle tool into either end of electrode. Keeping your fingers off the opposite end of electrode, slowly but firmly push the needle straight through the electrode's inner core until the needle tip appears at the other end of the electrode (be careful not to bend the needle inside the electrode). Wipe off any debris on the needle tip and gently pull the needle back out of the electrode. Wipe off the needle and remove any debris or moisture from the electrode surface.

CHLORIDE ELECTRODE

NEEDLE TOOL

2) Reinstall the electrode into the electrode housing in the correct order.

3) Install the electrode housing into the ISELyte Analyzer. Connect tubing and prime the ISE module completely. If the electrode's performance does not improve, contact your local Technical Support.

WARNING

NEEDLE TOOL can damage all other electrodes (K, Na, CO2, REF). Do not use!

Chloride Electrode Cleaning Procedure

Figure 14-1

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 14 of 15

REF

Reference

Electrode

Cl

GND

Electrode

CLEANING TOOL, P/N 03254

REFER TO INSTRUCTIONS IN

THE FIGURE 1

Chloride

Electrode

NOTE:

FOR CONVENIENCE SAKE, IT IS IMPORTANT TO DETACH THESE

ELECTRODES IN SMALL GROUPS FROM THE REST TO AVOID POSSIBLE

ACCIDENTAL DAMAGE WHEN HANDLING A LONG STRING OF

ELECTRODES. OBSERVE THE CLEANING PROCEDURE OUTLINED HERE

FOR OTHER ELECTRODES.

CLEANING PROCEDURE

1) Carefully insert the tip of 5ml - 10 ml syringe filled with appropriate solution onto the top duct of electrode assembly as shown.

2) Inject solution until all the solution is forced through interconnecting bores of the electrodes and flows out from the bottom electrode, thus thoroughly removing any contamination.

WARNING a) PUSH THE PLUNGER ONLY TO EJECT THE SOLUTION.

DO NOT PULL THE PLUNGER TO ASPIRATE THE

SOLUTION INSIDE ELECTRODES. THIS WILL CAUSE

IRREPARABLE DAMAGE AND CONTAMINATION TO

THE POTASSIUM ELECTRODE WITH Cl OR CO

2

PARTICLES. b) DO NOT USE PLASTIC STRING FOR CLEANING THE

REFERENCE OR Na ELECTRODES AS IT MAY DAMAGE

THE ELECTRODES INTERNALLY

Na

CO

2

Carbon

Dioxide

Figure 14-2

BI-DISTILLED

WATER

REFERENCE

SOLUTION

Sodium

Electrode

ISE ELECTRODES CLEANING PROCEDURE

K

Potassium

Electrode

REF

Reference

Electrode

Section - XIV Troubleshooting BT3000 PLUS Rev. 0, Soft Ver. 8 Page 15 of 15

SECTION XV

BT 3000 PLUS

SPARE PARTS FOR REPAIR AND MAINTENANCE

THE FOLLOWING IS LISTING OF SUBASSEMBLIES AND INDIVIDUAL PARTS,

WHICH ARE AVAILABLE FOR FIELD REPLACEMENT. INDIVIDUAL PARTS OTHER

THAN THOSE LISTED SUCH AS MECHANICAL PARTS, FASTENERS ETC. ARE

PROVIDED ONLY AS PART OF SUBASSEMBLY. THIS WIDE SELECTION OF SPARE

PARTS ALLOW THE SERVICE ENGINEER TO TAILOR CONFIGURATIONS TO

SPECIFIC REPAIR AND MAINTENANCE REQUIREMENTS AND TO PROVIDE

REPAIR FLEXIBILITY. PLEASE ASK IF YOU REQUIRE OTHER ITEMS NOT LISTED

HERE, AS THIS MAY BE POSSIBLE.

15-1. READING STATION MODULE SPARE PARTS

PART NO. DESCRIPTION

02765 LAMP RETAINING SPRING CLIP

03-04442-05 READING STATION - BT 3000 PLUS

06-04433-01 MASTER LOGIC MODULE

07-04334-03 CUVETTE ROTOR HUB ASSY

08-04347-01 TEMPERATURE & HALL SENSOR ASSY

08-04533-01 CUVETTE MOTOR ASSY

08-04695-01 QUARTZ PRISM WITH LENS ASSY

08-04981-02 PELTIER FAN ASSY

11-04779-01 CUVETTE PLATE WITH 34 CUVETTES

11-05083-01 CUVETTE FAN KIT

11-05136-01 MASTER POWER BOARD KIT

11-05137-01 MASTER POWER MODULE (ex 06-04434-01)

11-05209-01 CARTUCCIA POMPA PERISTALTICA (ex 330.9072)

15-04270-01 P.C.B. - READING TRAY INTERFACE

30-04570-01 CUVETTE PELTIER - A

30-04570-02 CUVETTE PELTIER - B

330.5708 "O"-RING FOR CUVETTE

330.8688

330.9321

330.9321A

TIMING BELT 150 TEETH FOR CUVETTE MOTOR

TUNGSTEN HALOGEN LAMP 12V, 35 WATTS, 9°

SOCKET FOR HALOGEN LAMP

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 1 of 10

WASHING MODULE

PART NO. DESCRIPTION

07-04850-01 WASHING MODULE ASSY

08-04405-01 WASHER PINCH VALVE ASSY (N.C.)

08-04569-01 PERISTALTIC PUMP ASSY FOR DILUTER

08-04572-01 FLUID CHAMBER PUMP

08-04573-01 STEPPING LINEAR ACTUATOR WASHING SYSTEM

08-04574-01 SAMPLING PINCH VALVE ASSY (N.C.)

08-04929-01 LIQUID SENSOR #WASHING STATION

11-04778-01 WASHING PISTON&PLUNGER ASSY

11-05046-01 DILUTER PUMP KIT (EX 08-04568-01)

15-04651-01 P.C.B. - OPTOCOUPLER

330.5703

662.0610

O-RING FOR DILUTER

WASHING PISTON KIT (ex 02586)

PHOTOMETER

PART NO. DESCRIPTION

04051

330.9336

330.9337B

330.9338

330.9339

330.9340

330.9342

330.9344

330.9345

330.9347B

662.1017

INTERFERENTIAL FILTER 700 NM

INTERFERENTIAL FILTER 340 NM

INTERFERENTIAL FILTER 380 NM

INTERFERENTIAL FILTER 405 NM

INTERFERENTIAL FILTER 436 NM

INTERFERENTIAL FILTER 478 NM

INTERFERENTIAL FILTER 510 NM

INTERFERENTIAL FILTER 546 NM

INTERFERENTIAL FILTER 578 NM

INTERFERENTIAL FILTER 630 NM

FLAT CABLE 16-16C FOR PREAMPLIFIER

06-05133-01 PHOTOMETER – COMPLETE (ex 662.1018)

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 2 of 10

15-2. REAGENT/REFRIGERATION SYSTEM SPARE

PART NO. DESCRIPTION

03275 REAGENT PULLEY WITH MAGNET

06-04435-03 REAGENT CONTROLLER MODULE

08-04537-01 CROSS BLOWER FAN ASSY

08-04633-01 REAGENT MOTOR ASSY

08-04699-01 REAGENT BOTTLES TRAY (MARKED 1-8)

08-04699-02 REAGENT BOTTLES TRAY (MARKED 9-16)

08-04699-03 REAGENT BOTTLES TRAY (MARKED 17-24)

08-04699-04 REAGENT BOTTLES TRAY (MARKED 25-32)

08-04699-05 REAGENT BOTTLES TRAY (MARKED 33-40)

11-04892-01 REAGENT BELT/PULLEY KIT (EX 330.8668)

11-05043-01 REAGENT HALL/TEMPERATURE SENSOR KIT (EX 08-04539-02,

08-04540-03)

11-05155-01 REAGENTS REFRIGERATION ASSY

11-05227-01 REFRIGERATOR INTERFACE BOARD KIT (ex 15-04680-01)

12-04711-01 REAGENT CONTROLLER CABLE

330.4605 PELTIER MODULE FOR REAGENTS

330.5709 "O"-RING Ø50.47 X 2.62

15-3. SERUM MODULE SPARE PARTS

PART NO. DESCRIPTION

02444 ANTISKID MAT FOR SERUM PLATE

04-04445-04 SERUM MODULE COMPLETE

06-04486-01 SERUM CONTROLLER MODULE

07-04893-01 SERUM PLATE WITH TUBE ADAPTERS

08-04349-01 SERUM MOTOR ASSY

08-04490-01 TENSION PULLEY SERUM

08-04681-01 SERUM PLATE COVER

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 3 of 10

PART NO. DESCRIPTION

11-05152-01 FUNNEL CAP WITH O-RING

12-04536-01 FLAT CABLE FOR PRIMARY TUBE SENSOR "RIGHT"

12-04538-01 FLAT CABLE FOR PRIMARY TUBE SENSOR "LEFT"

12-04714-02 SERUM CONTROLLER CABLE

15-04278-01 PRIMARY TUBE SENSOR (RIGHT)

15-04280-01 PRIMARY TUBE SENSOR (LEFT)

15-04282-01 HALL SENSOR ASSY #SERUM

15-04408-01 MOTOR DRIVER BOARD

20-04962-01 TUBE ADAPTER BUSHING #SERUM PLATE

20-04985-01 BOTTOM TUBE SPACER FOR SERUM PLATE

90-05201-01 FUNNEL CAP OPENER (TOOL)

330.8665 TIMING BELT 400 TEETH FOR SERUM PLATE DRIVE PULLEY

330.9351 BARCODE READER WITH CABLE

15-4. SAMPLER ARM SPARE PARTS

PART NO. DESCRIPTION

06-04436-03 LEFT SAMPLING ARM COMPLETE WITH ENCODER

06-04437-03 RIGHT ISE ARM COMPLETE WITH ENCODER

06-04872-01 LEFT SAMPLING ARM (WITH ENCODER) WITHOUT ARM HEAD

06-04873-01 RIGHT SAMPLING ARM (WITH ENCODER) WITHOUT ARM HEAD

07-04438-02 ARM HEAD WITH HEATER ASSY

08-04733-01 ARM SWIVEL MOTOR ASSY

08-04891-01 ARM UP/DOWN MOTOR ASSY

15-04408-01 MOTOR DRIVER BOARD

15-04765-01 P.C.B. - ARM CONTROLLER (LEFT ARM)

15-04765-02 P.C.B. - ARM CONTROLLER (RIGHT ARM)

15-04767-01 P.C.B. - ENCODER

20-04148-01 THUMB SCREW FOR NEEDLE

330.8694 TIMING BELT 80

330.8695

662.0629A

TIMING BELT 150

CLEANING TOOL FOR ARM NEEDLE

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 4 of 10

15-5. ISE MODULE SPARE PARTS

PART NO. DESCRIPTION

03011

03254

ISE PERISTALTIC PUMP ROTOR #08-04658-01

CLEANING TOOL FOR CHLORIDE ELECTRODE

06-04655-01 ISE MODULE WITH ELECTRODES

08-04574-01 ISE DILUTER PINCH-VALVE ASSY (NC)

08-04656-01 ISE 2-WAY PINCH-VALVE ASSY

08-04657-01 DILUTER PERISTALTIC PUMP

08-04658-01 ISE PUMP MOTOR

08-04659-01 ISE LAMP ASSY

08-04760-02 ISE PERISTALTIC PUMP TUBE #03011

08-04978-01 FLUID DISTRIBUTOR MANIFOLD

11-05046-01 ISE DILUTER PUMP KIT (EX 08-04568-01)

11-05135-01 ISE CONTROLLER BOARD KIT

12-04713-01 ISE INTERFACE CABLE

12-05045-01 EXTENSION CABLE FOR ISE DILUTER

15-04408-02 MOTORS DRIVER BOARD #ISE

15-04503-01 ISE INTERFACE BOARD

20-04484-01 PLATE FOR REAGENT BOTTLE

30-04763-01 ISE FILTER ASSY

330.5703

330.9072

662.0705

O-RING FOR DILUTER

CARTRIDGE FOR DILUTER PERISTALTIC PUMP #08-04657-01

ISE GROUND ELECTRODE

662.0711

662.0712

SODIUM (Na) ELECTRODE

POTASSIUM (K) ELECTRODE

662.0713 CHLORIDE (Cl) ELECTRODE

662.0716 CO

2

ELECTRODE

662.1024 ISE DISCHARGE ASSY

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 5 of 10

15-6. COMPUTER BOX

PART NO. DESCRIPTION

07-04487-01 MAINBOARD IBM PENTIUM WITH RAM, PROCESSOR AND FAN

08-04594-01 IBM COOLING FAN

08-04738-02 LCD DISPLAY MODULE COMPLETE WITH TOUCH SCREEN

11-05030-01 POWER SUPPLY 300W WITH BRACKET for Pentium IV board only

12-04584-01 IBM SUPPLY CABLE

12-04593-01 ON/OFF PUSHBUTTON ASSY

12-04740-01 POWER SUPPLY CABLE #TOUCH SCREEN

12-04741-01 CABLE FOR TOUCH SCREEN

12-04877-01 EXTENSION CABLE FOR TOUCH SCREEN

330.2144 HARD DISK DRIVE

330.2150 FLOPPY DISK DRIVE 3.5" 1.44 MB

330.2167B

330.7172

330.7173

330.7235A

330.7260

330.7281

330.9390A

330.9391

330.9391A

330.9391B

FLAT CABLE 40-40-40 (for connecting DVD/CD ROM Player and

Hard Disk to Mother Board)

IBM POWER SUPPLY FORTRON 200W

POWER SUPPLY FORTRON 300W

CPU COOLING FAN

DIMM 128 Mb (RAM)

DDR 256 Mb (RAM)

LCD DISPLAY 12.1" for Pentium III board only (old) for Pentium IV board only for Pentium III board only (old) for Pentium III board only (old) for Pentium IV board only

CONTROLLER FOR TOUCH SCREEN

INVERTER FOR LCD DISPLAY

CABLE FOR LCD INVERTER

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 6 of 10

15-7. CONSUMABLES

PART NO. DESCRIPTION

392

393

943

944

947

SURFACTANT WASH CONCENTRATE 2X50 ml

CUVETTE WASHING SOLUTION 1 LITER

ISE LOW CALIBRATOR 2 x 20 ml

ISE HIGH CALIBRATOR 2 x 20 ml

ISE STARTER KIT - 600 TESTS:

Buffer Conc., Reference Conc., Washing Solution, Enzymatic

Solution, ISE Calibrator Low, ISE Calibrator High

03254 CLEANING TOOL FOR CHLORIDE ELECTRODE

11-05209-01 PERISTALTIC PUMP CARTRIDGE (ex 330.9072)

11-05219-01 SIX-MONTHLY MAINTENANCE KIT (TUBING & ACCESSORIES)

(For analyzer with new Vacuum Pump System P/N 06-05161-01/02/03)

11-05220-01 ANNUAL MAINTENANCE KIT (TUBING & ACCESSORIES)

330.5703

330.5810

330.5811

330.5820

330.5823

330.9321

(For analyzer with new Vacuum Pump System P/N 06-05161-01/02/03)

O-RING FOR DILUTER

ELBOW FITTING 2.38mm (READING STATION)

ELBOW FITTING 3.2mm (READING STATION)

STRAIGHT FITTING 2.24mm (READING STATION)

ELBOW FITTING 1.58mm (READING STATION)

HALOGEN LAMP 12V/35W FOR PHOTOMETER

330.9614 TUBULAR FILTER FOR H

2

O TUBE

330.9615 H

2

O FILTER FOR WATER PUMP (REAR OF READING STATION)

662.1002 SIX-MONTHLY MAINTENANCE KIT (TUBING & ACCESSORIES)

662.1009

(For analyzer with Vacuum Pump System - "OBSOLETE" P/N 662.0788/C/D/E/F)

ANNUAL MAINTENANCE KIT (TUBING & ACCESSORIES)

662.1010

(For analyzer with Vacuum Pump System - "OBSOLETE" P/N 662.0788/C/D/E/F)

WASTE CONTAINER (10 LITERS CUBITAINER)

662.1016

667.1040

667.1072

667.1073

667.1074

667.1076

667.1080

BIMONTHLY MAINTENANCE KIT (ISE TUBING)

SERUM CAPSULE 2 ml

REAGENT CONTAINER 80 ml

REAGENT CONTAINER 50 ml

REAGENT BOTTLE 20 ml

REAGENT BOTTLE 10 ml

50 ml BOTTLE WITH SCREW CAP

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 7 of 10

15-8. ELECTRONICS HARDWARE

PART NO. DESCRIPTION

08-04546-01 LAMP FAN ASSY

08-04747-01 FLUID DISTRIBUTION MANIFOLD (REAR PANEL)

15-04431-01 PERIPHERAL INHIBITION BOARD

15-04661-01 POWER SUPPLY INTERFACE BOARD

330.2057

330.2132

CORDLESS KEYBOARD/MOUSE KIT

UPS 1100 VA

330.2172

330.6338

330.6340

330.6342B

PRINTER FOR BT 3000 PLUS

FUSE 250 VOLT, 0.5 AT

FUSE 250 VOLT, 2AT

FUSE 250 VOLT, 8AT

330.6400

330.6450

330.7171

POWER CORD FOR PERIPHERAL DEVICES

POWER ENTRY MODULE WITH SWITCH

LAMBDA POWER SUPPLY (400W)

330.7220A

330.7235A

330.9321

MAGNET FOR HALL SENSOR

CPU COOLING FAN

TUNGSTEN HALOGEN LAMP 12V, 35 WATTS, 9°

15-9. VACUUM PUMP SYSTEM SPARE PARTS

(REFER TO SECTION XII FOR PRODUCT ILLUSTRATIONS))

VACUUM PUMP SYSTEM P/N 06-05161-01/02/03

PART NO. DESCRIPTION

06-05161-01 VACUUM PUMP SYSTEM (COMPLETE) FOR BT3000 PLUS

08-05162-01 VACUUM PUMP ASSY

15-05085-01 VACUUM PUMP SYSTEM CONTROLLER BOARD

20-05145-01 INTERNAL WASTE CHAMBER (TRANSPARENT GLASS)

330.6312 POWER ENTRY MODULE

330.6338 FUSE 250 VOLT, 0.5 AT

330.6400 MAIN POWER SUPPLY CORDSET

330.9099 3-WAY SOLENOID VALVE

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 8 of 10

VACUUM PUMP SYSTEM P/N 662.0788/C/D/E/F (OBSOLETE)

PART NO. DESCRIPTION

08-04716-01 H

2

O TUBE WITH FILTER

08-04734-01 BLUE TUBE FOR WASTE

08-04735-01 BLACK TUBE FOR WASTE

15-04541-02 VAC.PUMP SYSTEM CONTROLLER BOARD

330.6312

330.6340

330.6342

330.6400

POWER ENTRY MODULE WITH FUSES

FUSE 250 VOLT, 2 AT (230 VAC VACUUM PUMP SYSTEM)

FUSE 250 VOLT, 4 AT (115 VAC VACUUM PUMP SYSTEM)

MAINS POWER SUPPLY CORDSET

330.9088

330.9614

330.9622

330.9623

330.9624

330.9625

330.9649

662.0777B

662.0777C

662.0788

CARTRIDGE # PERISTALTIC PUMP

TUBULAR FILTER FOR H

2

O TUBE

COUPLING BODY WITH SHUTOFF VALVE - FRONT PANEL

ELBOW FITTING FOR PERISTALTIC PUMP CARTRIDGE

COUPLING BODY STRAIGHT THROUGH FRONT PANEL

SINTERED BRONZE SILENCER

COUPLING INSERT FOR WASTE PROBE - FRONT PANEL

VACUUM PUMP 115 VAC WITH FITTINGS

VACUUM PUMP 230 VAC WITH FITTINGS

VACUUM PUMP SYSTEM (WITH 2 WASTE PROBES– 230 VAC)

662.0788E

662.0806

662.0807

662.0808

662.0808A

662.0810

662.0831

662.0831A

662.0831C

662.0831D

662.0831E

662.0831F

662.0831G

662.0831H

662.0832

662.0832A

VACUUM PUMP SYSTEM (WITH 2 WASTE PROBES– 110 VAC)

"RESTART" PUSH BUTTON (NOT WIRED)

HYDROPHOBIC FILTER ASSEMBLY

PERISTALTIC PUMP - LEFT

PERISTALTIC PUMP - RIGHT

VACUUM GAUGE ASSY

CABLE FOR POWER SUPPLY/BOARD 15-04541-02

CABLE FOR POWER SUPPLIES

FEMALE FISCHER CONNECTOR KIT

"RESTART" PUSH BUTTON KIT

GROUNDING CABLE FOR VAC PUMP

GROUNDING CABLE FOR POWER INLET

GROUNDING CABLE FOR POWER SUPPLY

GROUNDING CABLE FOR PUMP COVER

THE INTERNAL WASTE CHAMBER-LEFT

THE INTERNAL WASTE CHAMBER-RIGHT

(Schematics on the Last Pages of this Manual)

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 9 of 10

INFORMATION

Please contact Biotecnica Instruments S.p.A. for any configuration or special requirement not covered in this manual. For technical or ordering assistance start w ith our convenient ordering check list located in the above paragraph. For further assistance, don't hesitate to call the Biotecnica Instruments S.p.A. or your local sales/representative office.

To obtain accessories/spare parts, address order or enquiry to your

Biotecnica Instruments S.p.A. sales/service representative or to

Biotecnica Instruments S.p.A. and supply the follow ing Informations: a) Instrument Model and Serial Number b) Quantity of parts desired c) Part Number d) Description

Biotecnica Instruments S.p.A.

Via Licenza,18

00155 - Rome (ITALY)

Phone: +39 06 411 2316

Fax: +39 06 410 3079

E-mail: [email protected]

NOTE:

DUE TO IMPROVEMENTS IN DESIGN AND/OR SPECIFICATIONS, SOME

PRODUCTS MAY DIFFER SLIGHTLY FROM THE PREVIOUS DESCRIPTION.

IMPORTANT NOTICE:

THE BIOTECNICA INSTRUMENTS S.p.A. RESERVES THE RIGHT TO

MAKE CHANGES IN DESIGN AND SPECIFICATIONS WITHOUT NOTICE.

EVERY EFFORT HAS BEEN MADE TO PROVIDE ACCURATE

TECHNICAL AND PRODUCT INFORMATION. THE BIOTECNICA

INSTRUMENTS S.p.A. DISCLAIMS RESPONSIBILITY FOR ANY ERROR

OR OMISSION REGARDING TECHNICAL AND PRODUCT INFORMATION

PRESENTED IN THIS SERVICE MANUAL.

© 2003 BIOTECNICA INSTRUMENTS S.p.A., ROME,

ITALY

ALL RIGHTS RESERVED HEREIN AND NO PORTION OF THIS SERVICE

MANUAL MAY BE REPRODUCED WITHOUT THE PRIOR CONSENT IN

WRITING OF THE BIOTECNICA INSTRUMENTS S.p.A.

Section XV Spare Parts BT3000 PLUS Rev. 0, Soft Ver. 8 Page 10 of 10