Illumina Infinium Assay Glass Cleaning protocol
Below you will find brief information for Infinium Assay Glass. This document provides instructions on how to properly clean the glass back plates for the Tecan Flow-Through Chambers used in the Infinium Assay. Proper cleaning ensures consistent performance and optimal results. It includes detailed steps involving bleach soaking, rinsing, and drying following standard procedures outlined in the Infinium Assay Lab Setup and Procedures Guide.
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Key features
- Bleach soaking to remove contaminants
- Rinsing with deionized water
- Drying using standard protocol
- Use of dedicated cleaning equipment
- Procedure for periodic cleaning after every 7 uses or weekly
- Caution about bleach fumes in the BeadChip production lab
- Detailed steps for each cleaning stage
- Proper handling and disposal of materials
- Consistent performance of Infinium Assay
Frequently asked questions
You should use this new procedure to clean your glass back plates before running an Infinium BeadChip for the first time, and periodically after approximately every seven uses and/or every week depending on individual lab throughput.
You will need bleach, a Pyrex glass container, a fume hood, a Backplate Tecan Storage Rack, deionized water, and a 1L container for diluting the bleach.
Bleach fumes can degrade both the BeadChips and the fluorescent dyes used in the Infinium Assay. It's important to perform the cleaning in a location separate from the BeadChip production lab to avoid any damage to the equipment and reagents.
Refer to the Infinium Assay Lab Setup and Procedures Guide (Illumina part # 11207963) for the standard drying protocol.
Dilute bleach (5.25% sodium hypochlorite) with deionized water to 10% (0.525% hypochlorite).