Instructions for drawing samples from Human tissue freezer to laboratory

Instructions for drawing samples from Human tissue freezer to laboratory
Instructions for drawing samples from Human tissue freezer to
laboratory.
The following instructions apply only to Liz Hills laboratory CRPC 404, and
samples from the CONTAMED grant.
General considerations
Potential Hazard (1) Spillage of sample during transfer through building.
Corridors and stairs involved. Risk minimised by samples transported in sealable bags within a second sealed large plastic container. All samples to be
carried direct from freezer to category 2 hood.
Potential hazard (2) Risk of dropping equipment and samples during transfer
through building. Risk minimised by using plastic carrier with central handle
which leaves one hand free for opening doors. Do not carry too much in one
go several trips are recommended where necessary
Planning
Using the laboratory excel sheet of the samples held in freezer to plan your
work beforehand, so that you know exactly what you are asking to be
removed.
e-mail Majids laboratory ([email protected]) to check we can use their
category 2 hood on the day you require.
e-mail Julia who will liase when can take the samples from the designated
freezer on the day in question.
On day of transfer
Set up Majids category 2 hood ready to receive the samples see ’Instructions
for working in Majids Category 2 hood’.
Meet with Julia who will have the spill kit and take down to the Human tissue
designated freezer a sealed carrier half filled with crushed ice. Julia will give
you your samples and amend the database and laboratory records
accordingly, both of you will confirm the samples taken. You will pack the
samples in the sealed container and take them along with the spill kit straight
up to Majids laboratory.
ONCE THE SAMPLES HAVE BEEN GIVEN INTO YOUR CARE YOU ARE
RESPONSIBLE FOR THEM UNTIL THE SOLVENT EXTRACTION IS
COMPLETED AND THEY CAN BE CONSIDERED TO BE UNDER
DIFFERENT LEGISLATION.
Samples which have had solvent added are then considered to be under
‘Good laboratory practise’ rather than the HTA and can be stored in Liz’s
laboratory freezer.
Subdivision of unextracted tissue
If samples have been subdivided and any unextracted tissue remains it HAS
to be returned to the Human tissue designated freezer.
Organise to meet with Julia again, then again with the spill kit in hand take
remaining sample on ice in sealed container back to 2A1 where both of you
check the remaining tissue back into the freezer.
Once again Julia will be responsible for updating the database.
If there is unextracted tissue deal with this first (leaving extracted tissue in
Majids hood until you can get back upstairs again).
Draft July 09
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