Spectramax m5/m5 multi-detection reader e The SpectraMax

Spectramax m5/m5 multi-detection reader e The SpectraMax
SpectraMax M5/M5e multi-detection reader
A five-mode microplate reader with three-mode cuvette port
> five-mode reader with
wide range of applications
> three-mode cuvette port
> dual monochromators
> PathCheck sensor
> instrument and
software validation
> robotics compatibility
Corporate
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analyst
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analyst
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The SpectraMax® M5/M5e from Molecular Devices
is a dual-monochromator, multi-detection microplate
reader with assay performance similar to dedicated
single-mode readers. Detection modes include
UV-Vis absorbance (Abs), fluorescence intensity
(FI), fluorescence polarization (FP), time-resolved
fluorescence (TRF), and luminescence (Lum).
The reader also has a separate three-mode
cuvette port that enables Abs, FI, and Lum
readings. Monochromator tunability and highperformance detection capability make the
SpectraMax M5 multi-detection reader a valuable
tool in assay development and research as well as
in low- to medium-throughput screening. The
Corporate
Corporate
e has the added functionality
SpectraMax M5
of
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®
being certified for the CIS bio HTRF assays.
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Dual Monochromators
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With SpectraMax
M5/M5e, there is no analyst
need for
analyst
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expensive filters. The system uses two scanning
monochromators to determine optimal excitation
flex
flex
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and emission
settings. Changing methods
fluorophores requires only a few mouse clicks to
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optimize
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saving
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time saving
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Patented PathCheck Sensor
PathCheck® is the only patented† technology
available that measures the depth (optical pathlength)
of samples in a microplate. With SoftMax® Pro
software, PathCheck automatically normalizes the
well absorbance to a cuvette equivalent pathlength
of 1 cm—similar to using 96 or 384 cuvettes
simultaneously. PathCheck allows for the elimination
of standard curves and, for compounds with a known
absorptivity, concentration can be calculated directly
from absorbance.
superior optics
The optics in SpectraMax M5/M5e are designed to
provide optimal assay performance. The reference
diodes enable elimination of measurement noise due
to slight fluctuations in excitation light intensity.
The angled emission beam improves signal-to-noise,
especially in narrow Stokes shift fluorophores, by
reducing stray light. Elliptical mirrors are used
instead of lenses for maximum transmission with
minimal wavelength distortion. Top-quality UVgrade fibers give the highest light transmission down
to even the lowest wavelengths.
better results
cost saving
five-mode microplate reading with superior optics
top read head
from excitation
monochromator
reference diode
to emission
monochromator
and PMT
mirror
beamsplitter and
excitation polarizer
emission mirror
emission polarizers
absorbance detector diode
microplate
emission mirror
beamsplitter
beamsplitter
FL, TRF, FP excitation
excitation mirror
from
excitation
monochromator
FL, TRF, FP, Lum emission
reference diode
Abs
bottom read head
Applications
>
Endpoint, kinetic, spectrum, and area-well
scanning read types and Molecular Devices’
PathCheck Sensor allow homogeneous and
heterogeneous biochemical- or cell-based
microplate assays and assays that have multiple
readouts to be combined into one single
Corporate
experiment.
Applications include: Corporate
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> DNA/RNA/protein quantitation and purity
>ephys
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PicoGreen®/NanoOrange®/Bradford
assay
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> ELISAs/enzyme kinetics (e.g., Km, Ki, etc.)
>analyst
analyst
Drug dissolution profiles
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> Live/Dead® viability/cytotoxicity assays
>flex
Caspase-3 and protease assays flex
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> CatchPoint® cAMP assays
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IMAP® kinase assays
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>
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saving
>
>
>
>
>
>
>
reference diode
Intrinsic tryptophan fluorescence
Green fluorescent protein
FRET and TR-FRET assays
HTRF assays (SpectraMax M5e)
Reporter gene assays
ADME-Tox assays
Membrane
Permeability assays (PAMPA)
Corporate
Corporate
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™
FluoroBlok
cell migration assays 8222
Delfia® assays
to emission
monochromator
and PMT
from excitation
monochromator
Instrument and Software Validation
The SpectraTest™ ABS1 absorbance and
SpectraTest FL1 fluorescence validation packages
allow validation of optical characteristics. The
SoftMax Pro Software Validation Package and
IQ/OQ/PQ va­lidation protocols provide tools for
GLP, GMP, and FDA 21 CFR Part 11 compliance.
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comprehensive data analysis
analyst
analyst
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SoftMax8243Pro software provides data acquisition,
analysis and management capabilities, allowing
flex
flex analysis and custom calculations.
cross-plate
There
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is no need to export data to a spreadsheet program.
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time saving
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better results
cost saving
technical specifications
General Photometric Performance
Plate formats:
6, 12, 24, 48, 96, 384 wells
Light source:
Xenon Flash Lamp
(1 joule/flash)
Detectors:
2 photomultiplier tubes
Shaker time:
0 to 999 seconds
Temp. control:
2°C above ambient to 60°C
Temp. uniformity: < 1°C at 37°C set point
Temp. accuracy: ±1°C at 37°C set point
Endpoint reading : All modes
Kinetic reading : All modes
Spectral scanning: All modes
Well scanning:
Abs, FI, TRF, Lum
Absorbance Photometric Performance
Reading capabilities: Cuvette or microplate
Wavelength range:
200–1000 nm
Wavelength selection: Monochromator, tunable
in 1.0 nm increments
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range:
0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate):
< ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette):
< ±0.005 OD ±1.0%, 0–2 OD
Photometric precision:
< ±0.003 OD ±1.0%, 0–2 OD
Stray light:
< 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading capabilities: Cuvette or top or
bottom of a microplate
Wavelength range:
250–850 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Sensitivity: < 5 pM fluorescein in 96 wells or
cuvette, < 20 pM in 384 wells
Fluorescence Polarization Performance
Wavelength range:
400–750 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Precision: < 5 mP standard deviation at 1 nM
fluorescein in 96 and 384 wells
Time-Resolved Fluorescence Performance
Reading capabilities: Top or bottom of a
microplate
Wavelength range:
250–850 nm
Wavelength selection: Monochromators, tunable
in 1.0 nm increments
Bandwidth (EX, EM): 9 nm, 15 nm
Precision data collection: 1–100 flashes, delay
of 0–600 µsec. before read, integration time
selectable between 50–1500 µsec.
Sensitivity: 100 fM europium in 96 or 384 wells
with top-read
SpectraMax M5e only: Compliant with CIS bio
HTRF performance specifications
Robotics Compatibility
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SpectraMax readers can be integrated with an
optional plate stacker or robot from Molecular
Devices.
The StakMax™ microplate handling
Corporate
Corporate
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system8222
is a simple, easy-to-use stacker operated
from within SoftMax Pro. It can hold up to 50
ephys
ephys
plates with
barcode reading. Another option
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™
more advanced needs is the SynchroMax ET plate
analyst
analyst
handling
8243
8243 robot. With SynchroMax ET, a washer,
dispenser, and reader can be integrated into an assay
workstation
with a plate capacity of 320 plates,
flex
flex
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barcode reading, and simple scheduling capabilities.
SpectraMax
microplate readers are the number
one
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of our robotic partners. The plate carriage
saving
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time saving
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Luminescence Performance
Reading capabilities: Cuvette or top or bottom
of a microplate
Wavelength selection: All wavelengths or with
selected wavelengths
Wavelength range:
250–850 nm
Sensitivity: < 2 fg/well lower detection limit for
firefly luciferase in 96- and 384-well top read
Cross-talk:
< 0.3% in white 96- and
384-well microplates
Typical Read Times (minutes:seconds)*
96 wells 384 wells
Absorbance
0:18
0:49
Fluorescence Intensity
0:17
0:48
Fluorescence Polarization
0:42
2:03
Time-Resolved Fluorescence 0:17
0:48
Luminescence
2:00
7:00
*With 3 flashes/well in absorbance and fluorescence modes, and 1 sec./
well integration in luminescence.
General Specifications
Dimensions (in.):
8.6 (H) x 22.8 (W) x 15.3 (D)
Dimensions (cm): 22 (H) x 58 (W) x 39 (D)
Weight:
36 lbs. (16.4 kg)
Power consumption: < 420 watts
Power source:
100–240 Vac, 3 A, 50/60 Hz
Robotic-compatible: Yes
design has no springs or clamps, and its robotic
communication interface makes integration of the
SpectraMax M5/M5e straightforward.
Ordering Information
SpectraMax M5/M5e Multi-Mode Reader
> SpectraMax M5/M5e microplate reader
> SoftMax Pro software for Windows® and
Macintosh®
> SpectraTest ABS1 and/or FL1 validation plates
> StakMax microplate handling system
Contact your Molecular Devices sales representative
for configuration options.
better results
cost saving
SPECTRAMAX M5/M5 e MULTI-DETECTION READER
multi-mode microplate readers from Molecular Devices
SpectraMax M5/M5e
SpectraMax M2
SpectraMax M2e
Analyst® GT
FI, FP, UV/VIS Abs, TRF, Lum
FI, UV/VIS Abs
FI, UV/VIS Abs
FI, FP, UV/VIS Abs, TRF, Lum
TRF, Luminescence
TRF, Luminescence
tunable monochromator
tunable monochromator
tunable monochromator
8 Ex, 8 Em filters
wavelength range (Absorbance)
200–1000 nm
200–1000 nm
200–1000 nm
250–740 nm
wavelength range (Fluorescence)
250–850 nm
360–850 nm
250–850 nm
250–740 nm
4 nm for Abs
9 nm Ex, 15 nm Em for FI
4 nm for Abs
9 nm for FI
4 nm for Abs
9 nm for FI
variable (5–80 nm)
Abs, FI, Lum
Abs, FI
Abs, FI
No
6–384
6–384
6–384
96–1536
cuvettes & test tubes
Yes
Yes
Yes
No
endpoint read
Yes
Yes
Yes
Yes
kinetic read
Yes
Yes
Yes
Yes
multiple wavelength per well
Yes
Yes
Yes
Yes
wavelength-selectable
luminescence
Yes
No
No
Yes
spectral scanning
Yes
Yes
Yes
No
PathCheck Sensor
Yes
Yes
Yes
No
primary detection modes
secondary detection modes
wavelength selection
bandwidth
cuvettes
microplate formats
temperature control
Yes (ambient +2°C to 60°C) Yes (ambient +4°C to 45°C) Yes (ambient +4°C to 45°C)
No
integrated stacker or
SynchroMax ET
integrated stacker or
SynchroMax ET
integrated stacker or
SynchroMax ET
integrated stacker or
SynchroMax ET
FI, TRF, Lum
No
Yes
FI, TRF
FDA 21 CFR Part 11 compliance
Yes
Yes
Yes
No
robot-compatible
Yes
Yes
Yes
Yes
Yes (M5e only)
No
No
Yes
plate handling options
bottom-reading
HTRF certified
SALES AND SUPPORT
United States & Canada
Molecular Devices Corp.
Tel. +1-800-635-5577
Fax +1-408-747-3601
China
Molecular Devices Beijing
Tel. +86-10-6410-8629
Fax +86-10-6410-8601
Japan
Nihon Molecular Devices Osaka
Tel. +81-6-6399-8211
Fax +81-6-6399-8212
Australia
Molecular Devices Pty. Ltd.
Tel. +61-3-9896-4700
Fax +61-3-8640-0742
Molecular Devices Shanghai
Tel. +86-21-6887-8820
Fax +86-21-6887-8890
Nihon Molecular Devices Tokyo
Tel. +81-3-5282-5261
Fax +81-3-5282-5262
Germany
Molecular Devices GmbH
Tel. +49-89-9605-880
Fax +49-89-9620-2345
South Korea
Molecular Devices Korea, LLC
Tel. +82-2-3471-9531
Fax +82-2-3471-9532
Brazil
Molecular Devices Brazil
Tel. +55-11-3616-6607
Fax +55-11-3616-6607
United Kingdom
Molecular Devices Ltd.
Tel. +44-118-944-8000
Fax +44-118-944-8001
©2006 Molecular Devices Corporation. Printed in U.S.A. 9/06 Rep. 5K #0120-1367F+
Analyst, CatchPoint, IMAP, PathCheck, SoftMax, and SpectraMax are registered trademarks and SpectraTest, StakMax, and SynchroMax are trademarks of Molecular Devices Corporation. All other trademarks
are the property of their respective owners. Specifications subject to change without notice. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
www.moleculardevices.com
† The PathCheck Sensor is covered under
U.S. Patents 5,959,738, 6,320,662, 6,339,472,
6,404,501, 6,496,260 and 6,188,476.
SpectraMax M5/M5e is covered under U.S.
Patents 5,112,134, 5,766,875, 6,232,608,
6,236,456, 6,313,471 and 6,316,774.
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