SpectraMax M5/M5e multi-detection reader A five-mode microplate reader with three-mode cuvette port > five-mode reader with wide range of applications > three-mode cuvette port > dual monochromators > PathCheck sensor > instrument and software validation > robotics compatibility Corporate 8222 Corporate 8222 ephys 8181 ephys 8181 analyst 8243 analyst 8243 flex 8303 flex 8303 fliPr 8303 fliPr 8303 lifeSci flexible 8142 lifeSci labor 8142 The SpectraMax® M5/M5e from Molecular Devices is a dual-monochromator, multi-detection microplate reader with assay performance similar to dedicated single-mode readers. Detection modes include UV-Vis absorbance (Abs), fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), and luminescence (Lum). The reader also has a separate three-mode cuvette port that enables Abs, FI, and Lum readings. Monochromator tunability and highperformance detection capability make the SpectraMax M5 multi-detection reader a valuable tool in assay development and research as well as in low- to medium-throughput screening. The Corporate Corporate e has the added functionality SpectraMax M5 of 8222 8222 ® being certified for the CIS bio HTRF assays. ephys 8181 Dual Monochromators ephys 8181 With SpectraMax M5/M5e, there is no analyst need for analyst 8243 8243 expensive filters. The system uses two scanning monochromators to determine optimal excitation flex flex 8303 or 8303 and emission settings. Changing methods fluorophores requires only a few mouse clicks to fliPr fliPr 8303 optimize 8303 the system. saving lifeSci 8142 time saving lifeSci 8142 Patented PathCheck Sensor PathCheck® is the only patented† technology available that measures the depth (optical pathlength) of samples in a microplate. With SoftMax® Pro software, PathCheck automatically normalizes the well absorbance to a cuvette equivalent pathlength of 1 cm—similar to using 96 or 384 cuvettes simultaneously. PathCheck allows for the elimination of standard curves and, for compounds with a known absorptivity, concentration can be calculated directly from absorbance. superior optics The optics in SpectraMax M5/M5e are designed to provide optimal assay performance. The reference diodes enable elimination of measurement noise due to slight fluctuations in excitation light intensity. The angled emission beam improves signal-to-noise, especially in narrow Stokes shift fluorophores, by reducing stray light. Elliptical mirrors are used instead of lenses for maximum transmission with minimal wavelength distortion. Top-quality UVgrade fibers give the highest light transmission down to even the lowest wavelengths. better results cost saving five-mode microplate reading with superior optics top read head from excitation monochromator reference diode to emission monochromator and PMT mirror beamsplitter and excitation polarizer emission mirror emission polarizers absorbance detector diode microplate emission mirror beamsplitter beamsplitter FL, TRF, FP excitation excitation mirror from excitation monochromator FL, TRF, FP, Lum emission reference diode Abs bottom read head Applications > Endpoint, kinetic, spectrum, and area-well scanning read types and Molecular Devices’ PathCheck Sensor allow homogeneous and heterogeneous biochemical- or cell-based microplate assays and assays that have multiple readouts to be combined into one single Corporate experiment. Applications include: Corporate 8222 8222 > DNA/RNA/protein quantitation and purity >ephys ephys PicoGreen®/NanoOrange®/Bradford assay 8181 8181 > ELISAs/enzyme kinetics (e.g., Km, Ki, etc.) >analyst analyst Drug dissolution profiles 8243 8243 > Live/Dead® viability/cytotoxicity assays >flex Caspase-3 and protease assays flex 8303 8303 > CatchPoint® cAMP assays >fliPr IMAP® kinase assays fliPr > 8303 8303 lifeSci flexible 8142 lifeSci labor 8142 saving > > > > > > > reference diode Intrinsic tryptophan fluorescence Green fluorescent protein FRET and TR-FRET assays HTRF assays (SpectraMax M5e) Reporter gene assays ADME-Tox assays Membrane Permeability assays (PAMPA) Corporate Corporate 8222 ™ FluoroBlok cell migration assays 8222 Delfia® assays to emission monochromator and PMT from excitation monochromator Instrument and Software Validation The SpectraTest™ ABS1 absorbance and SpectraTest FL1 fluorescence validation packages allow validation of optical characteristics. The SoftMax Pro Software Validation Package and IQ/OQ/PQ validation protocols provide tools for GLP, GMP, and FDA 21 CFR Part 11 compliance. ephys 8181 ephys 8181 comprehensive data analysis analyst analyst fliPr 8303 fliPr 8303 8243 SoftMax8243Pro software provides data acquisition, analysis and management capabilities, allowing flex flex analysis and custom calculations. cross-plate There 8303 8303 is no need to export data to a spreadsheet program. lifeSci 8142 time saving lifeSci 8142 better results cost saving technical specifications General Photometric Performance Plate formats: 6, 12, 24, 48, 96, 384 wells Light source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes Shaker time: 0 to 999 seconds Temp. control: 2°C above ambient to 60°C Temp. uniformity: < 1°C at 37°C set point Temp. accuracy: ±1°C at 37°C set point Endpoint reading : All modes Kinetic reading : All modes Spectral scanning: All modes Well scanning: Abs, FI, TRF, Lum Absorbance Photometric Performance Reading capabilities: Cuvette or microplate Wavelength range: 200–1000 nm Wavelength selection: Monochromator, tunable in 1.0 nm increments Wavelength bandwidth: ≤ 4.0 nm Wavelength accuracy: ±2.0 nm Wavelength repeatability: ±0.2 nm Photometric range: 0–4.0 OD Photometric resolution: 0.001 OD Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD Stray light: < 0.05% @ 230 nm Fluorescence Intensity Performance Reading capabilities: Cuvette or top or bottom of a microplate Wavelength range: 250–850 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Sensitivity: < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells Fluorescence Polarization Performance Wavelength range: 400–750 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Precision: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 wells Time-Resolved Fluorescence Performance Reading capabilities: Top or bottom of a microplate Wavelength range: 250–850 nm Wavelength selection: Monochromators, tunable in 1.0 nm increments Bandwidth (EX, EM): 9 nm, 15 nm Precision data collection: 1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec. Sensitivity: 100 fM europium in 96 or 384 wells with top-read SpectraMax M5e only: Compliant with CIS bio HTRF performance specifications Robotics Compatibility Corporate 8222 Corporate 8222 ephys 8181 ephys 8181 analyst 8243 analyst 8243 flex 8303 flex 8303 fliPr 8303 fliPr 8303 lifeSci flexible 8142 lifeSci labor 8142 SpectraMax readers can be integrated with an optional plate stacker or robot from Molecular Devices. The StakMax™ microplate handling Corporate Corporate 8222 system8222 is a simple, easy-to-use stacker operated from within SoftMax Pro. It can hold up to 50 ephys ephys plates with barcode reading. Another option 8181 for 8181 ™ more advanced needs is the SynchroMax ET plate analyst analyst handling 8243 8243 robot. With SynchroMax ET, a washer, dispenser, and reader can be integrated into an assay workstation with a plate capacity of 320 plates, flex flex 8303 8303 barcode reading, and simple scheduling capabilities. SpectraMax microplate readers are the number one fliPr fliPr 8303 choice8303 of our robotic partners. The plate carriage saving lifeSci 8142 time saving lifeSci 8142 Luminescence Performance Reading capabilities: Cuvette or top or bottom of a microplate Wavelength selection: All wavelengths or with selected wavelengths Wavelength range: 250–850 nm Sensitivity: < 2 fg/well lower detection limit for firefly luciferase in 96- and 384-well top read Cross-talk: < 0.3% in white 96- and 384-well microplates Typical Read Times (minutes:seconds)* 96 wells 384 wells Absorbance 0:18 0:49 Fluorescence Intensity 0:17 0:48 Fluorescence Polarization 0:42 2:03 Time-Resolved Fluorescence 0:17 0:48 Luminescence 2:00 7:00 *With 3 flashes/well in absorbance and fluorescence modes, and 1 sec./ well integration in luminescence. General Specifications Dimensions (in.): 8.6 (H) x 22.8 (W) x 15.3 (D) Dimensions (cm): 22 (H) x 58 (W) x 39 (D) Weight: 36 lbs. (16.4 kg) Power consumption: < 420 watts Power source: 100–240 Vac, 3 A, 50/60 Hz Robotic-compatible: Yes design has no springs or clamps, and its robotic communication interface makes integration of the SpectraMax M5/M5e straightforward. Ordering Information SpectraMax M5/M5e Multi-Mode Reader > SpectraMax M5/M5e microplate reader > SoftMax Pro software for Windows® and Macintosh® > SpectraTest ABS1 and/or FL1 validation plates > StakMax microplate handling system Contact your Molecular Devices sales representative for configuration options. better results cost saving SPECTRAMAX M5/M5 e MULTI-DETECTION READER multi-mode microplate readers from Molecular Devices SpectraMax M5/M5e SpectraMax M2 SpectraMax M2e Analyst® GT FI, FP, UV/VIS Abs, TRF, Lum FI, UV/VIS Abs FI, UV/VIS Abs FI, FP, UV/VIS Abs, TRF, Lum TRF, Luminescence TRF, Luminescence tunable monochromator tunable monochromator tunable monochromator 8 Ex, 8 Em filters wavelength range (Absorbance) 200–1000 nm 200–1000 nm 200–1000 nm 250–740 nm wavelength range (Fluorescence) 250–850 nm 360–850 nm 250–850 nm 250–740 nm 4 nm for Abs 9 nm Ex, 15 nm Em for FI 4 nm for Abs 9 nm for FI 4 nm for Abs 9 nm for FI variable (5–80 nm) Abs, FI, Lum Abs, FI Abs, FI No 6–384 6–384 6–384 96–1536 cuvettes & test tubes Yes Yes Yes No endpoint read Yes Yes Yes Yes kinetic read Yes Yes Yes Yes multiple wavelength per well Yes Yes Yes Yes wavelength-selectable luminescence Yes No No Yes spectral scanning Yes Yes Yes No PathCheck Sensor Yes Yes Yes No primary detection modes secondary detection modes wavelength selection bandwidth cuvettes microplate formats temperature control Yes (ambient +2°C to 60°C) Yes (ambient +4°C to 45°C) Yes (ambient +4°C to 45°C) No integrated stacker or SynchroMax ET integrated stacker or SynchroMax ET integrated stacker or SynchroMax ET integrated stacker or SynchroMax ET FI, TRF, Lum No Yes FI, TRF FDA 21 CFR Part 11 compliance Yes Yes Yes No robot-compatible Yes Yes Yes Yes Yes (M5e only) No No Yes plate handling options bottom-reading HTRF certified SALES AND SUPPORT United States & Canada Molecular Devices Corp. Tel. +1-800-635-5577 Fax +1-408-747-3601 China Molecular Devices Beijing Tel. +86-10-6410-8629 Fax +86-10-6410-8601 Japan Nihon Molecular Devices Osaka Tel. +81-6-6399-8211 Fax +81-6-6399-8212 Australia Molecular Devices Pty. Ltd. Tel. +61-3-9896-4700 Fax +61-3-8640-0742 Molecular Devices Shanghai Tel. +86-21-6887-8820 Fax +86-21-6887-8890 Nihon Molecular Devices Tokyo Tel. +81-3-5282-5261 Fax +81-3-5282-5262 Germany Molecular Devices GmbH Tel. +49-89-9605-880 Fax +49-89-9620-2345 South Korea Molecular Devices Korea, LLC Tel. +82-2-3471-9531 Fax +82-2-3471-9532 Brazil Molecular Devices Brazil Tel. +55-11-3616-6607 Fax +55-11-3616-6607 United Kingdom Molecular Devices Ltd. Tel. +44-118-944-8000 Fax +44-118-944-8001 ©2006 Molecular Devices Corporation. Printed in U.S.A. 9/06 Rep. 5K #0120-1367F+ Analyst, CatchPoint, IMAP, PathCheck, SoftMax, and SpectraMax are registered trademarks and SpectraTest, StakMax, and SynchroMax are trademarks of Molecular Devices Corporation. All other trademarks are the property of their respective owners. Specifications subject to change without notice. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. www.moleculardevices.com † The PathCheck Sensor is covered under U.S. Patents 5,959,738, 6,320,662, 6,339,472, 6,404,501, 6,496,260 and 6,188,476. SpectraMax M5/M5e is covered under U.S. Patents 5,112,134, 5,766,875, 6,232,608, 6,236,456, 6,313,471 and 6,316,774.
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