LABCONCO FREEZE DRYER INSTRUCTIONS Before using the

LABCONCO FREEZE DRYER INSTRUCTIONS Before using the
LABCONCO FREEZE DRYER INSTRUCTIONS
December 15, 2008
C. MOODY
Before using the Freeze Dryer, please do the following:
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Wipe the interior of the collector chamber with a paper towel to remove any
accumulated moisture.
Check the collector chamber drain hose to ensure that the hose is free of
moisture and that the drain plug is securely installed.
Using a soft, lint-free cloth or paper towel, wipe the collector chamber lid
gasket to remove any dirt and contaminants that could cause a vacuum leak.
Vacuum grease is NOT required on the lid gasket.
Remove the drying chamber from the connection port and using a soft, lintfree cloth or paper towel, wipe the port gasket and sealing surfaces of the
drying chamber to remove any dirt and contaminants that could cause a
vacuum leak. Reinstall the drying chamber on the connection port. Vacuum
grease is NOT required on the port gasket.
• Check that each sample valve is closed, or in the “vent”
position.
Configuring the Freeze Dryer:
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To configure the Freeze Dryer, turn the power switch ON, and press “Menu.”
Press the “Select” button on the control panel until the desired function or unit
is flashing, then press “Menu” to save the selection and to move to the next
option for configuration. For “Vacuum Pump Start Up,” select MANUAL or
AUTO. For “Vacuum Units,” select mBar, Pa, or Torr. For “Collect Temp
Units,” select °C or °F.
Automatic Start-Up:
o Turn the Main Power switch ON. The refrigeration system will start
and the LCD display will read: “Auto Vacuum HI mBar” and “Wait
Collector: XX°C”
o If the Freeze Dryer was configured to run AUTO, the vacuum pump will
automatically start when the collector temperature reaches -40°C.
o The LCD display will show the actual temp. of the collector, and it will
read: “Run Collector: -40°C”
o The LCD display will read: “HI” if the vacuum in the system is above 5
mBar. Below 5 mBar, the display will show the actual vacuum value.
Manual Start-Up:
o Turn the Main Power switch ON. The refrigeration system will start
and the LCD display will read: “Manu Vac HI mBar” and “Wait
Collector: XXX”
o When the collector temp. reaches -40°C, the display will read: “Manu
Vac HI mBar” and “Ready Collector: -40°C”
o Press the vacuum switch. The vacuum pump will start and a typical
display reading will be: “Manu Vac 0.050 mBar” and “Run Collector:
-50°C”
o The LCD display will show the actual temp. of the collector, and will
indicate “HI” if the vacuum in the system is above 5 mBar. Below 5
mBar, the display will show the actual vacuum value.
Adding and Removing Samples to the Manifold:
o Pre-freeze samples. Shell freezing of samples is recommended for
wide mouth freeze drying flasks. Smaller samples in ampules and
serum bottles may be frozen in a freezer or in the center of the
collector chamber. The sample container size should always be at
least two to three times the sample size.
o Connect a pre-frozen sample to a sample valve on the manifold using
an adaptor. Be sure you are using the correct diameter adaptor for the
flask top and valve. Turn the plastic valve knob to the “VACUUM”
position to open the valve, which connects the attached sample to
system vacuum. The bevel on the knob should be positioned toward
the sample port to apply vacuum to the sample. This is the “Open”
position.
o Before adding another sample, allow the system vacuum to return to
0.133 mBar or lower. Any combination of valves and sample sizes
may be utilized at one time provided that the system vacuum and
collector temperature remain sufficiently low to prevent melting of the
frozen sample. Once you are finished adding samples, leave samples
to dry.
o When all the frost has disappeared from the outer surface of the
sample container and no cold spots can be detected by handling the
container, the sample is nearly dry. To be certain of low final moisture
content, dry the sample for several hours past this point.
o To remove a container after drying is complete, turn the plastic knob
on the valve to the “VENT” position, which closes the valve and vents
the container. (Should backfilling with an inert gas be required,
connect the gas supply line to the vent port on the valve before turning
the plastic knob on the valve to vent position). The sample container
may now be removed from the adaptor. In the vent position the bevel
on the knob should point away from the sample port. This is the
“Closed” position.
o Ampules may be flamed sealed while connected to a valve by using a
sealing torch. Care must be taken not to burn the valve. An insulation
material should be placed between the valve and the torch.
Shut Down Procedure:
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At the end of a run, or when a sufficient amount of condensate accumulates
on the collector coil to obstruct the flow of vapor to the collector chamber, the
Freeze Dryer should be defrosted.
o All samples should have been removed at this point. Release system
vacuum by turning the plastic knob on a valve to the open or
“VACUUM” position.
o Press the Vacuum Switch on the control panel to turn the vacuum
pump OFF. Turn OFF the Main Power Switch on the right side of the
cabinet.
Defrosting Procedure:
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Pull the collector chamber drain hose out from the left hand side of the Freeze
Dryer and remove the drain plug. Place the drain hose in a suitable container
to collect the condensate that will melt off the collector coil.
Remove the collector chamber lid and allow ambient room air to melt the ice.
Dispose of the liquid appropriately.
Flush the collector chamber with water and wipe chamber dry.
Reinstall the drain hose plug and slide drain hose back into the side of the
cabinet.
Please contact Celeste @x3756, Rm. 256, if you have any questions. ☺
FREEZE DRYER TIPS TO REMEMBER:
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The volume of the freeze dry flask should be two to three times the volume of the
sample.
When the Freeze Dry system is started, the valves should all be in the vent
position. (The bevel on the knob should point away from the sample port). This
will allow vacuum to build up in the system.
Always vent the vacuum line before turning off the vacuum pump.
Make sure all vacuum lines are secured to prevent leaks.
Wear appropriate protective attire and eye protection.
Clean stainless steel condenser after use.
Do not work with damaged, scratched, or chipped glassware.
The gas ballast should be closed during the operation of the Freeze Dry System.
Make certain that the oil in the vacuum pump is at the proper level.
When using flammable or hazardous solvents, the vacuum pump must be vented
to or operated inside a fume hood.
If a sample is spilled in the collector chamber, it must immediately be cleaned up.
If using chemical compounds that may cause degradation to some Freeze Dryer
parts, it is imperative that the equipment susceptible to degradation be
thoroughly cleaned after use. They should be removed and flushed with water.
Please contact Celeste @x3756, Rm. 256, if you have any questions.
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