user manual pco.edge family pco. PCO asks you to carefully read this manual before using the pco.edge camera system and follow the instructions. In case of any questions or comments, please contact us at PCO. telephone +49 (0) 9441 2005 50 fax +49 (0) 9441 2005 20 email [email protected] postal address PCO AG Donaupark 11 93309 Kelheim, Germany The cover photo shows an exemplary pco.edge CLHS camera. The lens is sold separately. Copyright © 2016 PCO AG (called PCO in the following text), Kelheim, Germany. All rights reserved. PCO assumes no responsibility for errors or omissions in these materials. These materials are provided as is without warranty of any kind, either expressed or implied, including but not limited to, the implied warranties of merchantability, fitness for a particular purpose, or non-infringement. PCO further does not warrant the accuracy or completeness of the information, text, graphics, links or other items contained within these materials. PCO shall not be liable for any special, indirect, incidental, or consequential damages, including without limitation, lost revenues or lost profits, which may result from the use of these materials. The information is subject to change without notice and does not represent a commitment on the part of PCO in the future. PCO hereby authorizes you to copy documents for non – commercial use within your organization only. In consideration of this authorization, you agree that any copy of these documents, which you make, shall retain all copyright and other proprietary notices contained herein. Each individual document published by PCO may contain other proprietary notices and copyright information relating to that individual document. Nothing contained herein shall be construed as conferring by implication or otherwise any license or right under any patent or trademark of PCO or any third party. Except as expressly provided, above nothing contained herein shall be construed as conferring any license or right under any PCO copyright. Note that any product, process, or technology in this document may be the subject of other intellectual property rights reserved by PCO, and may not be licensed hereunder. Released: August 2016 © PCO AG pco.edge family User Manual V2.23 © PCO AG, Germany TOP TABLE OF CONTENTS 1. INTRODUCTION 5 1.1 INTENDED USE 5 1.2 DATA OVERVIEW 6 2. SAFETY INSTRUCTIONS 7 3. SYSTEM COMPONENTS 8 4. INSTALLATION 9 4.1 DRIVER 9 4.2 CAMWARE 10 5. QUICK START 11 5.1 PREPARATION 11 5.2 START 11 5.3 YOUR FIRST IMAGE 12 6. CAMWARE 4 SOFTWARE 13 6.1 CHAPTER OVERVIEW 13 6.2 CAMERA OVERVIEW / LIST 14 6.3 CAMERA PROPERTIES 6.3.1 TIMING 6.3.2 ROLLING SHUTTER 6.3.3 GLOBAL SHUTTER 6.3.4 GLOBAL RESET 6.3.5 IMAGE SIZE 6.3.6 SENSOR CONTROL 6.3.7 RECORDING CONTROL 6.3.8 I/O SIGNALS TAB 6.3.9 CONVERT CONTROL 16 17 20 24 27 29 31 32 34 36 6.4 IMAGE OVERLAY 38 6.5 RECORDER TOOLS 39 6.6 VIEW WINDOW 41 6.7 RECORDER (IMAGES) 42 6.8 SETTINGS OVERVIEW 6.8.1 AUTO SAVE 44 45 6.9 CAMWARE MENU TABS & FEATURES 6.9.1 DEMO MODE 6.9.2 FILE MENU 6.9.3 CAMERA MENU 6.9.4 ACQUISITION MENU 6.9.5 VIEW MENU 6.9.6 WINDOW MENU 6.9.7 HELP MENU 6.9.8 RIGHT-CLICK MENU 6.9.9 ADDITIONAL FEATURES 47 47 48 50 51 52 52 53 54 56 3 APPENDIX 57 A1 TECHNICAL DATA 58 A1.1 STANDARD VERSION 58 A1.2 WATER COOLED STANDARD VERSION 58 A1.3 CAMERA LINK HS VERSION 59 A1.4 SPECIFICATIONS 60 A1.5 MOUNTING A1.5.1 INSTABLE MOUNTING A1.5.2 CORRECT MOUNTING A1.5.3 COOLING 60 60 61 61 A1.6 REVERSE WITH CAPTION 62 A2 CHANGE FROM F-MOUNT TO C-MOUNT 63 A3 WATER COOLING OPTION PCO.AQUAMATIC II 64 A3.1 SYSTEM COMPONENTS 64 A3.2 FIRST TIME INSTALLATION 65 A3.3 OPERATION 66 A3.4 DIMENSIONS 67 A3.5 YOUR OWN COOLING SYSTEM 67 A4 INTERFACES 68 A4.1 CABLE POSSIBILITIES 68 A4.2 CAMERA LINK A4.2.1 INSTALLATION OF FRAME GRABBER A4.2.2 MICRO DIAGNOSTICS TOOL A4.2.3 IMAGE DATA FLOW 69 69 70 71 A4.3 CAMERA LINK HS 73 A4.4 USB 3.0 A4.4.1 DRIVER INSTALLATION A4.4.2 HARDWARE RECOMMENDATIONS A4.4.3 USB 3.0 FAQ A4.4.4 INSTALLATION OF THE USB 3.0 CARD A4.4.5 RING BUFFER AND FIFO 74 74 74 76 78 79 A5 BINNING WITH CMOS SENSORS 80 A6 LIGHTSHEET SCANNING MODE 81 A7 IMAGE FILE FORMATS 82 A8 CUSTOMER SERVICE 84 A8.1 SERVICE 84 A8.2 MAINTENANCE 84 A8.3 RECYCLING 84 A8.4 TROUBLE SHOOTING 85 A9 INDEX 86 ABOUT PCO 87 4 1 INTRODUCTION 1. INTRODUCTION Advantages of the pco.edge family The pco.edge family is a breakthrough in scientific imaging cameras. It has the distinctive ability to simultaneously deliver extremely low noise, high frame rates, wide dynamic range, high quantum efficiency, high resolution and a large field of view - all in one image. The camera’s main features (model-specific) • • • • • • • ultra low noise down to 0.8 electrons med high resolution up to 5.5 megapixel best dynamic range up to 40000:1 high-speed up to 100 fps @ full resolution high quantum efficiency up to 80% flexibility user selectable choice of shutter mode free of drift stabilized Peltier cooling in order to avoid any drift phenomena in image sequences 1.1 INTENDED USE This camera system is designed for use by technicians, engineers and scientists. It is a scientific measuring instrument, which provides images. The camera may only be used according to the instructions of this manual. The disclosures and operating conditions in these operating instructions must be respected. Unauthorized modifications and changes of the device are forbidden for safety reasons. Areas of Application • • • • • • • • • • • • live cell microscopy single molecule detection localization microscopy lightsheet microscopy selective plane illumination microscopy SPIM structured illumination microscopy SIM TIRF microscopy / waveguides spinning disk confocal microscopy genome sequencing (2nd and 3rd gen) FRET 5 • • • • • • • • • • • • • • FRAP lucky imaging astronomy adaptive optics solar astronomy fluorescence spectroscopy bio- & chemiluminescence high content screening photovoltaic inspection x-ray tomography ophthalmology flow cytometry biochip reading machine vision spectral (hyperspectral) imaging • laser induced breakdownspectroscopy (LIBS) 1.2 DATA OVERVIEW Overview – Available camera models This table shows an overview over all available camera models. Type pco.edge 3.1 Interface USB 3.0 pco.edge 4.2 LT USB 3.0 pco.edge 4.2 USB 3.0 Read Out Frequency Rolling 105 MHz 24.99 50 Global 204 MHz 12.86 50 Global Reset 105 MHz 24.99 50 Rolling 110 MHz 24.06 40 Global Reset 110 MHz 24.06 40 Rolling 110 MHz 24.06 40 Global Reset 110 MHz 24.06 40 95.3 MHz (slow scan) 27.77 35 272.3 MHz (fast scan) 9.76 100 100 MHz 26.58 36 274 MHz 9.68 101 Rolling 86 MHz 30.51 30 Global 160 MHz 16.40 28 Global Reset 86 MHz 30.51 30 95.3 MHz (slow scan) 27.52 33 286 MHz (fast scan) 9.17 100 286 MHz 9.17 50 95.3 MHz (slow scan) 27.52 33 286 MHz (fast scan) 9.17 100 100 MHz (slow scan) 26.24 35 286 MHz (fast scan) 9.17 100 286 MHz 9.17 50 100 MHz (slow scan) 26.24 34 286 MHz (fast scan) 9.17 95 pco.edge 4.2 Camera Link Rolling pco.edge 4.2 Camera Link HS Rolling pco.edge 5.5 USB 3.0 Rolling pco.edge 5.5 Camera Link Global Global Reset Rolling pco.edge 5.5 Camera Link HS Global Global Reset 6 Line time (µs) Shutter FPS Sensor mono & color mono mono mono mono mono & color mono & color mono & color 2 SAFETY INSTRUCTIONS 2. SAFETY INSTRUCTIONS CLASS 1 LASER PRODUCT (only pco.edge CLHS) Risk of injury due to dazzle. Do not point the laser beam at persons. Do not look into the laser beam or at direct reflexes. Manipulations of the laser device are not allowed. DANGER DAMAGED POWER CABLE OR POWER PLUG Danger to life due to electric shock. Each time the camera is used, check the power cable for damage. WARNING ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE Risk of injury due to electric shock. Never slide any items through slits or holes into the camera. CAUTION CONDENSATION Risk of injury due to electric shock if condensation enters the camera. To avoid the risk of water condensation, protect the camera against extreme changes of ambient temperature. CAUTION TRIPPING HAZARD Risk of injury from tripping over loose cables. Never position the cable in a way that it could become a tripping hazard. NOTICE HUMID OR DUSTY ENVIRONMENTS Humidity, dust or X-rays could damage the camera. Never operate the camera in humid or dusty environments or in places with high amounts of X-ray radiation. NOTICE JOLT & VIBRATION To prevent damage to the camera, the system must be kept stable and protected against strong jolts or vibrations. Use the mounting threads of the camera to mount the camera stable. NOTICE LENS MOUNTING Do not force the lens onto the camera. To protect the lens connector thread from damage, use minimal force when attaching a lens to the camera. NOTICE LIQUIDS DAMAGE CAMERA If liquids have penetrated the device. Immediately switch off the camera, separate it from power line and contact our customer support. NOTICE DAMAGED CAMERA HOUSING If the camera has been dropped or the casing is damaged. Immediately switch off the camera, separate it from power line and contact our customer support. 7 3. SYSTEM COMPONENTS The camera system includes the following parts. Camera Head F-mount optical connection (standard) For standard F-mount / SLR lenses and adapters. C-mount ring provided (see appendix A2) For standard C-mount and microscopy connectors. DC Power Jack (connect to power supply) Input/Output 4x SMA connectors 2x input - 2x output Interface (user selectable) LED indicates camera status Green continuous: camera is booting Green blinking: camera is ready for operation Yellow blinking: recording on error Red blinking: Serial Number Tag (on the bottom of the camera) Mounting Thread 1/4-20 UNC mounting threads Camera Link Grabber Card / USB 3.0 PCI Interface Card PCI Express x4 Card (Camera Link / HS) or PCI Express x1 Card (2 x USB 3.0 connections) A PCI Card with 4 x USB 3.0 connections is also available, contact PCO for further details. A PCIe x4 slot is necessary for this card. Power Supply Your system will be equipped with either a 24 V or a 12 V power supply, depending on the model you selected. (connector: Lemo FGG.0B) Cable CameraLink Cable (2x3m) / USB-A/USB-B cable (3m) / FOL cable Digital Camera Tools (USB storage device content) • • • Camware: software for camera control & image acquisition Camera driver & tools Software development kit (SDK) & demo programs in C and C++ 8 4 INSTALLATION 4. INSTALLATION You will find all necessary files on the accompanying USB storage device. You may also download the latest versions of our software, camera driver and third party software drivers from the PCO website. Minimum system requirements: • • • Intel® Core™ i7 RAM > 8 GB DDR3 Windows 7 or higher • • • Full-HD resolution display PCI Express x4 Gen 2 (CLHS) PCI Express x4 Gen 1 (CL) Please contact PCO for an appropriate system configuration. 4.1 DRIVER There are three different kinds of interfaces available: Camera Link & Camera Link HS (Frame Grabber) When operating the camera with Camera Link Interface: Please run the appropriate grabber driver installation with default settings. For detailed installation instructions please see A4.2 (Camera Link) and A4.3 (Camera Link HS) USB 3.0 When USB 3.0 is used as a camera interface, it is recommended to use the enclosed PCIe Interface card. For detailed installation instructions or further hardware recommendations, see A4.3. Graphic card NVIDIA Cuda Driver: 1 Please update your NVIDIA driver for Camware 4. In case of an old driver version GPU Processing is not working. Therefore image processing is slow. Please check if GPU Processing is activated by having a look into the Proc config settings 2 in the Convert Control window 1 (see Convert Control chapter 6.3.9). If GPU Processing is disabled and shown grayed, please update your NVIDIA driver or check the website of the computer manufacturer for graphic card driver updates. Your NVIDIA driver version must be at least 333.11 or higher. 2 9 4.2 CAMWARE The Camware Windows application software enables you to control every camera parameter or setting. Images can be displayed on a monitor and may be downloaded and stored. The USB storage device contains the installation files for the software for latest Windows operating systems in 32 & 64 bit. After a successful installation, you will find the program folder Digital Camera Toolbox in your program directory and a Camware32/64 button on your desktop. Other helpful tools are also installed in the same directory. To uninstall the Camware program, please use the Software feature under Windows’ System Control. Please follow the installation wizard •1 Install Camware as Admin to install to program folder, instead it will be installed only to user folder •2 Then choose install directory •3 Choose components: Select additional drivers for Camera Link Interface (Silicon Software Dll ME IV) •4 After the next two screens installation is complete 1 2 3 4 10 5 QUICK START 5. QUICK START In order to get familiar with your new camera and software it might be helpful, if you first aim at an object that is easy to focus and that can be seen at standard light conditions. 5.1 PREPARATION • Computer is turned on • Installation is finished (see chapter 4) • An appropriate lens is attached (remove cap) or the camera is attached properly to the microscope, spectrograph or other scientific device • Camera is connected to the PC (USB 3.0 or Camera Link) • Camera is connected (green LED blinks) to the power supply and ready 5.2 START Start Camware and the graphical user interface will start up: NOTE Always install latest Camware version to be able to use full function of your pco camera. 11 5.3 YOUR FIRST IMAGE 2 3 Please follow the instructions: 1 • Camware must be started. 1 • A view window 2 is shown automatically /or open a new one. • Start live preview. 3 • Right-click in the view window and apply Auto Range Peak. 4 • You may adjust exposure time 5 , aperture and focus. 4 • Now you should clearly 6 see the object in the window. 5 6 If you need to change exposure time (e.g. the image is still either too dark or too bright), please go to chapter 6.3.2. If you want to record and save images, please see chapter 6.3.7 and chapter 6.6 for detailed information. NOTE Live preview: Useful for fast and easy camera adjustment and focusing. Does not record or store images. 12 6 CAMWARE 4 SOFTWARE 6. CAMWARE 4 SOFTWARE PCO’s Camware is an excellent software for camera control, image acquisition and archiving of images in various file formats. This chapter provides a detailed description of all Camware functions. Camware works with any kind of PCO camera. Please see PCO website for the latest version of this software. 6.1 CHAPTER OVERVIEW Chapter 6.2: lists all cameras connected 6.2 Camera Overview/List Preview, connected cameras, recording profiles Chapter 6.3 Camera Properties: main dialog for all camera settings: 6.3.1 Timing 6.3.2 Rolling Shutter 6.3.3 Global Shutter 6.3.4 Global Reset 6.3.5 Image Size 6.3.6 Sensor Control 6.3.7 Recording Control 6.3.8 I/O Signals 6.3.9 Convert Control Dialog Timing settings / Trigger modes / Synchronization Explanation / Timing Explanation / Timing Explanation / Timing ROI / Sensor format / Binning CDI / Double shutter / Temperature Recorder aquire mode / Timestamp / Sequence trigger Input - Output Contrast, Saturation, Gamma… Chapter 6.4/ 6.5 /6.6 /6.7 / 6.8 describe the recording functions 6.4 Image Overlay 6.5 Recorder Tools 6.6 View Window 6.7 Recorder (Images) 6.8 Settings Overview Overlay for recorded images Record / Play and Settings View window functions Preview of recorded images Overview of all property settings / Auto File Save Chapter 6.7 describes the available tabs (File, Camera Acquisation, View, Window, Help) the right-click menu and additional features. 6.9.1 Demo Mode 6.9.2 File 6.9.3 Camera 6.9.4 Acquisation 6.9.5 View 6.9.6 Window 6.9.7 Help 6.9.8 Right-click menu 6.9.9 Additional features 13 No camera connected Open, save and print files Camera control; Rescan, Setup… Live preview; Acquire secquence; Rec. memory settings; Rec. settings New window; Convert Control New / close / split window Logfiles, Support file, About Zoom; Flip; Mirror; Rotate… White Balance, Contrast, ROI by Mouse, Short Cut list 6.2 CAMERA OVERVIEW / LIST If closed, the Camera Overview window can be opened by selecting the View tab and Toolbars and Docking Windows → Camera Overview. 1 1 3 4 5 2 6 The Camera Overview window allows you to manage several connected cameras. It displays a list of all connected PCO cameras. Camware is able to scan 2 for connected cameras or close a connected camera. It is possible to define many different settings for each camera (max. 30 sets per camera → add new set 3 ). New view windows 44 can be opened and the Live Preview 5 can be started. When unfolded, the Preview shows a small preview window 6 (always monochrome) integrated to the camera list. Live preview enables the adjustment of aperture and focus and to have a first look at your object. During live preview no images are saved into the computer RAM. During Live preview Trigger and Acquire mode are set to Auto. Camera Setting: All Preferences that have been made in the Camera Properties (see 6.3) are saved to Camera Settings. Define different Settings with different Preferences in Camera Properties for each of your experiments. Settings can be switched easily at any time (not during record) and can be copied to other cameras. Link Preview Set to ‘Preview’ When Link Preview Set to Preview is activated the Preview set with its parameters will always be active when you start a Live Preview 5 . In case this function is deactivated, the Live Preview will always show live images with the parameters of your active setting. Setting a higher exposure time for Preview set and linking it to the preview function can be a great advantage if preview light conditions are different from those in recording situations. 14 6 CAMWARE 4 SOFTWARE Click and drag camera setting: If you want to copy e.g. Camera Setting 1 to Camera Setting 4, just drag & drop Setting 1 to Setting 4 and Camware will ask if you want to copy the settings. It is possible to copy each setting to every camera. Master Sets This function facilitates the image acqusition with multiple cameras. Define two or more Master Sets to switch during an experiment very easily between different predefined settings for each camera. Each image acquisition or experiment can be recorded with its own Master Set. To display Master Sets, please right-click in the Camera Overview window and select Show Master Sets. 1 3 2 Master Set window Define different Master Sets. Select individual Camera Settings within each Master Set. Functions: Add Master Set 1 or Remove active master 2 . Put it to active status by clicking on one of your sets 3 . Important Setting (for cameras without internal memory) Memory Allocation Dialog If you want to change the number of recorded images in Camware, you have to open the Acquisition Tab (see 6.9.4) and choose Recorder Memory Settings. This sets the number of images recorded in one sequence. The maximum is defined by approved RAM size. 15 6.3 CAMERA PROPERTIES The Camera Properties window in Camware is the main interface for all camera settings. The active set selected within Camera List can be adjusted here. The former main instance Camera Control (known from Camware 3.x) and the Convert Control (see 6.3.9) can be opened additionally. Three view options with various functions can be selected: Standard, Custom and Expert. 1 3 2 4 Standard mode 1 only shows camera name, type, set, serial number and exposure time. Exposure time is the only parameter that can be changed. It is recommended for new Camware users. Custom mode 2 shows several more setting possibilities and functions can be hidden or shown by the Custom Properties Button. 4 Additional to the standard mode many more options are selectable. Expert mode 3 (for advanced users) shows all possible camera property settings. An explanation for every setting is displayed below the properties dialog. 16 6 CAMWARE 4 SOFTWARE 6.3.1 TIMING Introduction to timing An important parameter for a camera is the frame rate. The upper limit of the frame rate is defined by exposure and readout time. The figure below shows the timing scheme. Exposure and readout are done simultaneously, this means while image n is readout from the sensor, image n+1 is already integrated within the sensor’s pixel elements. Figure 1 shows, that in case of short exposure times, the readout is the limiting factor. The second figure 2 shows that for long exposure times the exposure time plus delay (tdelay) is the limiting factor. n+1 readout n+1 exposure texp Figure 1 treadout : Image timing for short exposure times n+1 n+1 exposure texp Figure n+2 n-1 readout n+2 2 tdel : Image timing for long exposure times If a lower frame rate is desired, this can be achieved by inserting additional delay times. These rules also apply in external trigger mode, i . e . this defines when the next trigger can be applied or recognized. For further timing explanations (e.g. fps based timing) please read the following chapters for Rolling Shutter, Global Shutter and Global Reset. 17 Trigger Mode 1 In this context trigger means exposure trigger, i.e. the trigger signal controls the exposure of a single image (light integration time). Auto Sequence: The camera will optimize the image recording to achieve the best possible frame rate. In the auto sequence exposure control mode, the camera determines the fastest possible frame rate depending on the adjusted exposure time and the required readout time. After a start command is given, the sequential recording is started until a stop command is given. 1 Soft Trigger: Single images can be recorded with this Camware command. A single image can be acquired by pressing the Single Trigger button. This button appears after pressing the Start Record button (see 6.5 ). Other signals cannot influence this operating mode. NOTE If the trigger rate of the external signal is quite near the maximum possible frame rate (difference < 1/1000), it will be random, whether or not a trigger is accepted! In the External Exposure Start exposure control mode, single image recording is started by the falling or rising edge of the voltage signal at the SMA input #1(see 6.3.8). The frame rate cannot be set, as the frame rate is defined by the frequency of the external signal. However the predefined exposure time and ROI settings affect the maximum possible frame rate. The Busy Status signal at SMA #3 (SMA explanation see 6.3.8) will indicate whether a new trigger is accepted. Exp trig (in) Exp Stat (out) Busy Stat (out) acc. not acc. accepted accepted The maximum achievable frame rate in external trigger mode is negligibly less (about 0.1%) than operating the camera in Auto Sequence mode. If the trigger rate of the external signal is higher than the maximum possible frame rate, every second trigger pulse is ignored. Therefore the actual frame rate drops to half of the external trigger rate. If the trigger rate is increased further, then only every third, every fourth etc. trigger edge will be accepted. 18 6 CAMWARE 4 SOFTWARE Exp trig (in) Exp Stat (out) Busy Stat (out) acc. not acc. acc. not acc. acc. not acc. acc. not acc. In order to avoid trade-offs at maximum frame rate use either the Busy Status signal or make sure that the external trigger rate follows this condition: 0.999 x External Trigger Rate ≤ fmax. External Exposure Control An external signal applied at SMA #1 (see 6.3.8), controls the start and the duration of the exposure. A new exposure is started by the falling or rising edge of the voltage signal at the SMA input. The exposure is finished when the opposite edge is detected. Thus in this mode, the start as well as the length of the exposure time can be controlled. No further settings can be made, as the image timing is completely controlled by the external trigger signal. Exp trig (in) Exp Stat (out) Busy Stat (out) treadout acc. accepted not acc. accepted Be aware, that the externally controlled exposure time is limited. The integration will be stopped automatically if the maximum exposure time is achieved. The Busy Status signal at SMA #3 (see 6.3.8) indicates if a new trigger is accepted. Camera Interface pco.edge 3.1 USB 3.0 pco.edge 4.2LT USB 3.0 pco.edge 4.2 CL / CLHS pco.edge 4.2 USB 3.0 pco.edge 5.5 CL / CLHS USB 3.0 19 Shutter Mode Rolling Global Global Reset Rolling Global Reset Rolling Rolling Global Reset Rolling Global Global Reset Max.exposure time 2s 100 ms 2s 10 s 2s 10 s 20 s 2s 2s 100 ms 2s 6.3.2 ROLLING SHUTTER In Rolling Shutter mode the pixel reset and exposure start is carried out row by row. Each row has the same exposure time, but a different start and end of exposure. The pco.edge image sensor consists of two discrete halves, which are exposed and read out simultaneously, i.e. from the outside to the center by default. Within one row, the exposure starts simultaneously for all pixels. Available RS readout modes see chapter 6.9.3. General Timing Diagram reset start reset end readout start tglobal tframe readout end tframe texposure (first row) t t texposure (last row) tall rows The exposure time of each row starts with the corresponding reset of the row. Then after a predefined time, the exposure is stopped. The light induced accumulated charge carriers of the pixels in a row are recorded into memory in a low noise (readout) mode. This results in the total image appearing in memory corresponding to the row readout. Timing The exposure and delay time can be adjusted in steps of one line time (see p.6). Camera (RS only) pco.edge 3.1 pco.edge 4.2 LT Interface USB 3.0 USB 3.0 Camera Link & CLHS USB 3.0 CL & CLHS & USB 3.0 pco.edge 4.2 pco.edge 4.2 pco.edge 5.5 20 Exposure time 500 µs … 2 s 100 µs … 10 s 100 µs … 10 s 100 µs … 20 s 500 µs … 2 s Delay time 0…1s 6 CAMWARE 4 SOFTWARE FPS based (only Camera Link interface) The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible. Only for Auto Sequence trigger mode and only available with Camera Link Interface First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to 1 / treadout. The frame rate can be adjusted in steps of 1 mHz (Rolling Shutter). If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate. Camera Interface pco.edge 4.2 (@ full resol.) CL pco.edge 5.5 (@ full resol.) CL Frame rate (FPS Based) 0.1…35 Hz @ 95.3 MHz 0.1…100 Hz @ 272.3 MHz 0.1…100 Hz @ 272.3 MHz 0.5…100 Hz @ 286 MHz Exposure time 100 µs…10 s 500 µs…2 s Exposure time > Sensor frame readout time (Auto Sequence) In case the required exposure time is longer than the frame readout time, the image sensor is completely exposed to light for some time (tglobal). In case of a triggered flash illumination, this would be the best moment to illuminate the image sensor. t exposure 1st row, 2nd image t exposure 1st row, 1st image Trigger (in) Start NOTE Δ t = t : 1 line time (See p.6) Busy (out) Start Expos (out) Start Global (out) Start tframe t all rows 1st t all rows 2nd 21 Δt =t The hardware signal for the time tglobal is available on connector #4 (Global out see 6.3.8). Obviously, if during exposure and readout, parts of the viewed image are moving horizontally, this would result in image distortion. This is why the global shutter mode may be a prerequisite for some applications. However, most dynamic events can be captured in 1 ms, which is a common integration time with SLR cameras set at 1/1000 exposure. The time shift from one row to another is only about 10 µs (fast scan). The resulting maximum readout time of 10 ms (at full resolution) seems to be sufficient for a broad spectrum of dynamic events. The 10 ms is also faster than the image shift process of most frame transfer emCCD image sensors previously used for low light applications. If this does not influence the image recording and processing, then rolling shutter mode will not affect it either. Exposure time < Sensor frame readout time (Auto Sequence) In case the required exposure time is shorter than the frame readout time, the image is composed of two exposure bands moving from the outside to the center of the sensor. exposure stop & readout band of simultaneous exposure reset & exposure start For example, the shortest exposure time in RS is 500 µs (pco.edge 3.1 & 5.5) and 100 µs (pco.edge 4.2). The band of simultaneous exposure is in this case (smallest possible height) at full resolution: e.g. pco.edge 4.2: 100 µs / 24.93 µs (line time (see p.6)) = 4 → number of simultaneous rows = 8 (Rolling Shutter mode A-D see 6.9.3) Previous comments on image distortion (also known as Rolling Shutter Effect) apply here as well. Graph on next page. 22 6 CAMWARE 4 SOFTWARE Auto Sequence graph Trigger (in) Start Busy (out) Start Expos (out) Start tframe tall rows 1st image tall rows 2nd image Details for External Exp. Start and External Exp. Ctrl The detailed timing for external trigger includes system delay times, an adjustable additional delay time, and the jitter. NOTE The jitter tjit can be a maximum of one row/line time. Exp trig (in) Exposure trsys tjit trsys tfsys tdelay tdelay jitter time rising edge time falling edge delay time tjit tfsys tdelay tjit ≤1 line time see p.6 0 µs … 1 s For optimized synchronization (minimized jitter time) use the falling edge of the line signal at the status output (see 6.3.8). System times are depending on your camera settings and can be read out from your camera, for further information see SDK manual PCO_GetImageTiming. 23 6.3.3 GLOBAL SHUTTER NOTE Global Shutter is not available for pco.edge 4.2. First, all pixels are globally reset and these reset values are shifted into so-called diffusion nodes. From there, they are non-destructively read out into memory as reset dark images. The exposure starts after transfer of the reset dark image to the diffusion nodes, where they are stored on the chip. The exposure is stopped by global charge transfer to the diffusion nodes. Then, the exposure image is read out to the memory, where the former reset dark image is subtracted to perform an external correlated double sampling, which reduces the noise. Since two images have to be read out to receive one resulting image, the sCMOS image sensor’s Global Shutter mode has only half of the frame rate of the Rolling Shutter mode. Reset Exposure Readout Timing The exposure and delay time can be adjusted in steps of one line time (see page 6 ). Camera (GS only) pco.edge 3.1 pco.edge 5.5 pco.edge 5.5 Interface USB 3.0 CameraLink/CLHS USB 3.0 Exposure time 20 µs … 100 ms 10 µs … 100 ms 20 µs … 100 ms Delay time 0 µs … 1 s FPS based (only edge 5.5 Camera Link) The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible. Only for Auto Sequence trigger mode and only available with Camera Link Interface. First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to 1 / treadout. Minimum frame rate is 1 / max. exposure time. The frame rate can be adjusted in steps of 1 mHz (Global Shutter). If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate. Camera Interface Frame rate (FPS Based) CL 10 … 50 Hz @ 286 MHz pco.edge 5.5 24 Exposure time 10 µs … 100 ms 6 CAMWARE 4 SOFTWARE External Exposure Start (Auto Sequence respectively) Trigger (in) Busy (out) Exposure (out) Frame # of lines line time1 ROI(y) ∗ 𝑙𝑙𝑙𝑙𝑙𝑙𝑙𝑙 𝑡𝑡𝑡𝑡𝑡𝑡𝑡𝑡 2 Programmable: 1 line time1 … 100 ms (tframe – texp) line time1 tjit tframe texp tdelay (system) tif 1 line time → see p.6 The listed parameters can be output via SDK function Get Image Timing dependent on the selected ROI. NOTE If texp < tframe system delay (tdelay) is added before exposure starts. 25 External Exposure Control Trigger (in) Busy (out) Exposure (out) Frame # of lines line time1 ROI(y) ∗ 𝑙𝑙𝑙𝑙𝑙𝑙𝑙𝑙 𝑡𝑡𝑡𝑡𝑡𝑡𝑡𝑡 2 Counted line time1 tjit tframe texp 1 line time → see p.6 In External Exposure Control trigger mode the external signal controls start of image acquisition and duration of the exposure. First, all pixels are globally reset and these reset values are shifted into so-called diffusion nodes. From there, they are non-destructively read out into memory as reset dark images. In this mode, the exposure starts always after the readout of the dark image is completed. The length of the exposure has been detected by the sensor from the trigger input. The exposure is stopped by global charge transfer to the diffusion nodes after the respective time. Then, the exposure image is read out to the memory, where the former reset dark image is subtracted to perform an external correlated double sampling, which reduces the noise. Since two images have to be read out to receive one resulting image and the exposure cannot start during readout time of the dark image, this specific Global Shutter mode provides less than half of the frame rate of the Rolling Shutter mode. 26 6 CAMWARE 4 SOFTWARE 6.3.4 GLOBAL RESET NOTE Global Reset is not available for pco.edge 4.2 CL / CLHS. All pixels are globally reset and the exposure starts for all rows at the same time. The exposure stop is carried out row by row; therefore the duration of the exposure is not the same for all pixels. The rolling readout improves the image quality, but due to the difference in exposure time, a flash illumination is recommended. The readout (exposure stop) is done from the outside to the center. General Timing Diagram readout start reset / exposure start t global readout end t frame t exposure (first row) t exposure (last row) t all rows The exposure time of all rows starts simultaneously. The exposure time of the first row stops after the predefined time, the following rows are read out from the outside to the center row by row. Please note that this leads to a different duration of exposure time for all rows. Timing The exposure and delay time can be adjusted in steps of one line time (see p.6). Camera (GR) pco.edge 3.1 pco.edge 4.2 / pco.edge 4.2 LT pco.edge 5.5 / Interface Exposure time USB 3.0 30 µs – 2 s Delay time 0 µs … 1 s Camera Link / CLHS pco.edge 5.5 27 10 µs – 2 s FPS based (only edge 5.5 Camera Link) The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible. Only for Auto Sequence trigger mode and only available with Camera Link Interface. First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to 1 / treadout. The frame rate can be adjusted in steps of 1 mHz (Global Reset). If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate. Camera (GR only) Interface Frame rate (FPS Based) Exposure time pco.edge 5.5 Camera Link 1…33.3 Hz @ 95.3 MHz 1…100 Hz @ 286 MHz 10 µs…2 s Details for External Exp. Start and External Exp. Ctrl The detailed timing for external trigger includes system delay times, an adjustable additional delay time and the jitter. Exp trig (in) Exposure trsys tjit trsys tfsys tdelay tdelay jitter time rising edge time falling edge delay time tjit tfsys tdelay tjit ≤1 line time see p.6 0 µs … 1 s For optimized synchronization (minimized jitter time) use the falling edge of the line signal at the status output (see 6.3.8). System times are depending on your camera settings and can be read out from your camera, for further information see SDK manual PCO_GetImageTiming. 28 6 CAMWARE 4 SOFTWARE 6.3.5 IMAGE SIZE Region of Interest 1 The ROI (Region of Interest) selects only a part of the sensor to be read out. Vertical ROI: In order to speed up the frame rate and to reduce the amount of image data, the selected ROI needs to be placed symmetrical to the horizontal center line. 1 2 Horizontal ROI: In order to reduce the amount of image data a horizontal ROI can be set. Please be aware, that changes in horizontal direction will not increase the frame rate. The decreased image size you see within Camware is a combination of reduced sensor resolution and software downsizing (SoftROI). 3 Basic Setting: Activate Basic Setting by clicking on … to easily set a centered ROI by just keying in the resolution in pixels. ROI window Activate ROI window by clicking on … after selecting the ROI (symm. horz./vert.) menu or use the … right to the X Res / Y Res and click on ROI window. The ROI window will open and it is possible to set a new Region of Interest by dragging a window with the mouse or by keying in the values. 29 Software based ROI (Soft-ROI) (not avaiable for USB3.0) This function calculates the Region of Interest via software. Due to this functionality, the resolution of pco.edge cameras with Camera Link interface can be adjusted in steps of 1 – 4 pixels. Since the readout architecture of CL cameras is not able to address single pixels, this downsizing is done by software. If you work with Camware Device Adapters (µManager, Labview…) or with PCO SDK, the Soft-ROI is disabled by default. For further information, please see the SDK description. ROI table with Soft-ROI disabled by default Interface ROI, horizontal increments ROI, vertical increments Min. ROI Vert. symm. ROI USB 3.0 4 1 64x16 no pco.edge 4.2 CL 20 2 40x16 yes pco.edge 4.2 CLHS 16 1 64x16 no pco.edge 4.2 / 4.2LT USB 3.0 4 1 64x16 no pco.edge 5.5 CL 160 2 160x16 yes pco.edge 5.5 CLHS 16 1 64x16 no pco.edge 5.5 USB 3.0 4 1 64x16 no Camera pco.edge 3.1 ROI table with Soft-ROI Option enabled (only Camera Link) pco.edge 4.2 pco.edge 5.5 CL CL 1 4 1 1 40x16 160x16 no no Open regedit to control Soft-ROI: Under HKeyCurrent-User/Software/PCO/ create a DWORD value with the name SoftROI for each camera. Set this value to 1 in order to enable Soft-ROI. Remove or set this value to 0 in order to disable Soft-ROI by default. Keep in mind that unsymmetrical ROI decreases FPS. Table below shows values for SoftROI enabled. Binning 2 Binning combines neighboring pixels (in either the horizontal or vertical direction) to form super pixels. It increases the signal to noise ratio (SNR), reduces the readout noise of the resulting pixels and decreases the spatial resolution of the total image, which is recorded. For further information see A5. (pco.edge 5.5 CL(HS): Binning only in Rolling Shutter mode) Available Binning Modes: H1xV1, H1xV2, H1xV4, H2xV1, H2xV2, H2xV4, H4xV1, H4xV2, H4xV4. 30 6 CAMWARE 4 SOFTWARE Format 3 Camera pco.edge 3.1 pco.edge 4.2 pco.edge 4.2 / 4.2LT pco.edge 5.5 Interface USB 3.0 CL CLHS / USB 3.0 CL / CLHS / USB 3.0 Preselected Sensor Format 2048 x 1536 2060 x 2048 2048 x 2048 2560 x 2160 6.3.6 SENSOR CONTROL Pixelclock 1 The pixel clock sets the clock frequency and therefore the image sensor readout speed. (See pco.edge family overview table on page 6 for available readout frequencies.) 3 B/W Noise Filter 2 In addition to the integrated static defect pixel list processing a dynamic noise filter can be activated here in order to remove so-called blinkers and high noise pixels. If you encounter unexpected aliasing effects, turn this filter off. 1 2 For the following settings please start Camera Control 33 and select Sensor (Misc.) Tab: Conversion Factor The conversion factor defines how many charge carriers (electrons), which have been generated by light in the image sensor in each pixel, are necessary to generate one count (one intensity level) in the digital image. Therefore, the conversion factor describes the gain that is applied to the signal before it is converted into a digital value. The conversion is optimized for the pco.edge @ 0.46 e-/count. Cooling Setpoint Display of sensor temperature: A peltier cooling unit is used to keep the sensor's dark current to an acceptable minimum and in order to allow for a continuous operation free of any drift phenomena in image sequences. Either an internal fan or an external water cooling system assures proper heat transfer from the peltier element to regulate the temperature of the cameras. Camera Interface Sensor Temperature pco.edge 4.2 USB 3.0 0 °C pco.edge 3.1/5.5 USB 3.0 5 °C pco.edge 4.2 LT USB 3.0 10 °C pco.edge 4.2/5.5 Camera Link 5 °C pco.edge 4.2/5.5 CLHS 7 °C 31 6.3.7 RECORDING CONTROL Recorder Mode 1 Camware will use free RAM space on your computer. The recorded images will be temporarily saved as 16bit multi TIFF. In Sequence mode the recording stops when RAM space is full. In Ring Buffer mode the camera will stop only by a stop command, hence overwriting previous images. For longer recording periods an appropriate RAID system is necessary, see also the Direct Record to File option, see 6.9.2. Acquire Mode 2 1 3 2 The acquire mode gives you the ability to enable or disable the recording by an external signal. If set to Auto all images are accepted and all images taken are saved. A signal at the acq enbl input (see chapter 6.3.8) is ignored for this function. Operation of the acquire mode depends on the selected trigger mode. If set to External, the camera will only record images if the external signal enables recording. Trigger mode Auto Sequence: This sensor timing scheme is paused by the signal at the acq enbl input. The acq enbl input is sampled at the beginning of the image generation, which can be seen at the rising edge of the busy stat output. If the acq enbl input is high (low, when inverted) when an image is acquired, it causes an idle state until the acq enbl input is low (high, when inverted). Busy Stat out) Acq enbl (in) image image image image In trigger mode External Exp. Start, the acq enbl input works like a gate for the trigger signal. A trigger edge (rising, falling when exp trig is inverted) is accepted only when the acq enbl signal is high (low, when inverted). Acq enbl (in) Exp trig (in) Busy Stat (out) accepted not acc. not acc. accepted accepted In trigger mode External Exp. Ctrl, the acq enbl input works very similar to the mode External Exp. Start. However, the acq enbl input is ignored for the edge which is closing the exposure time (started exposure will be finished accordingly). 32 6 CAMWARE 4 SOFTWARE When using acq enbl in external trigger modes, the following timing specification should be met: Exp trig (in) tsu = 70ns th = 70ns Acq enbl (in) tsu th If the acq enbl signal changes within the window of tsu (set up) to th (hold), the behavior is random. The trigger may be accepted or ignored. Sequence Trigger Once, a falling or rising edge at the acquire enable trigger input (see chapter 6.3.8) is recognized, an internal image counter starts to run. It will count all acquired images and will stop the recording when the predefined number of images is reached. Example timing diagram: Trigger mode = external exposure start; Acquire mode = sequence trigger; Image counter = 3 Acq enbl (in) Exp. trig. (in) Status busy (out) not acc. Image 1 Image 2 Image 3 not acc. Timestamp 3 A time stamp can be placed into the upper left corner of the image. It can be either put off, binary or binary with text. The time resolution is 1μs. In binary mode the first 16 pixels will be filled with the time stamp information (binary code). The numbers are coded in BCD with one byte per pixel, which means that every pixel can hold 2 digits. If the pixels have more resolution than 8 bits, then the BCD digits are right bound adjusted and the upper bits are zero. (1 BCD digit ≙ 4 bits; 2 numbers ≙ 2 BCD ≙ 8 bits = 1 byte; every pixel can hold 2 digits) For further information please refer to the SDK. In binary and ASCII mode text will be placed into the image replacing the content of the image (271x 8 pixels). Time step shows the end of exposure time. Three different information is stamped onto the image: number of the image 1 , date 2 and time 3 . 1 33 2 3 6.3.8 I/O SIGNALS TAB Start Camera Control (see 6.3) and select the I/O Signals Tab. 1 2 3 4 1 2 3 4 Exposure Trigger 1 If checked, a signal for External Exp. Start or External Exp. Ctrl trigger mode (see chapter 6.3.1) is accepted at the exp trig SMA input #1. Aquire Enable 2 If checked, a signal for Acquire Mode or Sequence Trigger Mode (see chapter 6.3.7) is accepted at the acq enbl SMA input #2. Status Busy 3 If checked, a signal indicating busy status is given at the status busy output. Once an acceptable trigger edge is received, busy will go on status high. As soon as busy goes low again, a new trigger edge is accepted. Status Expos 4 If checked, a signal indicating exposure status is given at the status output. Status Expos indicates the actual exposure window for one frame. Status Line 4 If checked, a signal indicating line status is given at the status output. Use the falling line edge for optimized synchronization (minimized jitter time; see page 23). Polarity Type active for high/low signal or rising/falling edge Maximum low level: 0.8V Minimum high level: 2V Slew Rate >1ms/V TTL Electrically grounded, no opt coupler. 3.3 Volt LVTTL out , short time short-circuit-proof. Continuous overvoltage withstand: +10V and -5V. 1ms pulse overvoltage withstand: +33V and -33V ESD pulse: +- 4kV 34 6 CAMWARE 4 SOFTWARE Rolling Shutter signal type At the I/O-signal tab Rolling Shutter signal type options are available (only Status Exposure and if Shutter is set to Rolling Shutter, see 6.9.3 Setup. There are four signal types available: •1 Shows the exposure time of the first rolling shutter line (tfirstline) •2 Shows when all sensor lines are exposed (tglobal) •3 Shows the exposure time of the last rolling shutter line (tlastlinel) •4 Shows if any sensor line is integrating (talllines) 1 2 4 3 35 6.3.9 CONVERT CONTROL Start the Convert Control Dialog with the Black/White Button in Camera Properties. Convert Control BW The user can influence how the 16 bit intensity values of the original image are displayed in 8 bit values in different ways. BW Settings (includes histogram of original data) It is possible to hide the histogram of original data 1 and to switch tab/histogram 2 . 4 1 Green sliders in histogram 3 left slider = Min controller (corresponds to value 0 of the 8 bit display). Values below that mark are set to 0, i.e. displayed as black. right slider = Max controller (corresponds to value 255). Values above that mark are set to 255, i.e. displayed as white. 2 3 The values in-between are converted into a value between 0 and 255 according to Contrast and Gamma settings. See the small graph 4 , which reflects the calculation. Proc config tab: please see under Convert Control Color Converted Hist This tab shows converted data. 36 you the histogram of 6 CAMWARE 4 SOFTWARE Convert Control Color (only pco.edge color) Color Balance (Histogram of original data) Intensity of single color can be controlled by Saturation and Vibrance 1 . 1 2 3 7 4 Press the Auto button to set the white balance 2 . 5 The balancing of RGB can be controlled by Col.Temp and Tint 3 . 6 It is possible to hide the histogram of original data 4 and to switch tab/histogram 5 . The user can influence how the 16 bit intensity values of the original image are displayed in 8 bit values in different ways. White sliders in histogram 6 left slider = Min controller (corresponds to value 0 of the 8 bit display). Values below that mark are set to 0, i.e. displayed as no color. right slider = Max controller (corresponds to value 255). Values above that mark are set to 255, i.e. displayed as full color. The values in-between are converted into a value between 0 and 255 according to Contrast and Gamma settings. See the small graph 7 , which reflects the calculation. Proc. Config (Process configuration) Due to proprietary high-end algorithms used for these image processing features, no detailed description is given here 1 1 3 2 4 2 3 4 GPU Processing On: Switch on in order to significantly reduce processing time (increases refresh rate of the live image. Fast pco debayering: only color cameras Color Refine Filter only color cameras Noise Reduction NLM: Non local means algorithm Denoise Adaptive: only color cameras Sharpen: On: only color cameras (first activate Fast pco debayering Converted RGB Hist This tab shows you converted data. 37 the histogram of 6.4 IMAGE OVERLAY 1 Open Image Overlay: you can easily switch between Camera Properties and Image Overlay with these two buttons. 1 If not available, please see 6.9.5 View Menu to activate this menu. This function enables an individually configurable image overlay, which allows to display information within the images. Many different options are available by clicking Add item to… 2 2 Also the Appearance is configurable: Font, Text color, Text opacity, Background color, Background opacity and horizontal or vertical orientation. 3 A preview of the image overlay is shown. 3 4 Each item can be moved upwards, downwards or deleted by clicking on … 4 By drag & drop the Image Overlay can be moved easily to your favorite position within an image. To activate image overlay, right-click in the image window and activate Show Image Overlay. 5 5 NOTE This function does not overwrite image data. 38 6 CAMWARE 4 SOFTWARE 6.5 RECORDER TOOLS Recorder Tools provides Record and Play function, Play Settings and Record Settings. It can be found on the right lower side of Camware or, if closed, activated by View Menu (see chapter 6.9.5) Record Start/Stop record: with Record Button. Recording: in record state Camware software is highlighted in red. It is possible to change the exposure time during record. See 6.3 Camera Properties. Software Trigger Mode: after record is started an arrow pointing downwards applies a single trigger (see 6.3.1). Play Settings 1 1 Play Speed: selectable play speed from x1 to x256 or from 1fps to 16fps. E.g. in mode x1 a recording with 1000 fps is played with 25fps. 1 fps means that only one frame per second is played. Play Mode: selectable play mode of the recorder (continuous or single time (re)play). 2 Play Direction: selectable direction of record play (forward or backward) Record Settings 2 Averaging: if in the dropdown list a value not equal to x1 is selected, the corresponding number of images is averaged in the buffer, reducing the statistically independent noise. IIR Lowpass: another option to reduce the noise is the activation of the Infinite impulse response IIR lowpass filter. This filter takes 90% of the previous image and 10% of the new image to create images with clearly reduced noise. Image (actual) = Image (act - 1) * 0.9 + Image (new) * 0.1 39 Reminder dialog: If you have already made a recording but you did not save it, Camware will ask you to save the record before starting a new one. Extended Recorder can be activated (see 6.9.5) Recording with multiple cameras: If all cameras are activated the recording is started simultanously for all cameras. Recorder will use Recorder mode settings (Sequence or Ring Buffer) of the seleted camera for all cameras (see 6.3.7) For single camera recording, deactivate cameras by removing the check mark from the box. 1 1 40 6 CAMWARE 4 SOFTWARE 6.6 VIEW WINDOW More View Windows It is possible to open more view windows of one camera: just click on new view window 1 and Camware will create a new one 2 . Even when multiple view windows (or from multiple cameras) are open, the same image number is always shown in all of the view windows. 2 1 3 A dropdown menu 3 helps to select a view window. If you have more view windows than can be displayed on the desktop, you will be able to select each view window. Split View Window 4 5 The view window can be split. Choose Window → Split 44 and a split cross will be shown. You can easily adjust the size of the splitted window elements by grabbing and dragging the dividing lines 55 . The main function is that you are able to view four regions of your image in one view. Choose the Zoom± function to zoom in the image (first turn off Stretched View) (See 6.9.8) If you want to undo the split, you have to double click on the 6 deviding line (after symbol 6 is visible). Two Tabs side by side or on top of each other If you want to view two tabs side by side or arranged one above the other just drag a tab and then Camware will ask you if you want to create a new horizontal or vertical tab group. Undo this very easily by draging the tab back to its former position. This also applies for view windows of several cameras. 41 6.7 RECORDER (IMAGES) When recording is done, small preview images (thumbnails) are built and displayed automatically. This will take some time depending on the performance of your computer system and of the interface used. 1 If you click (left mouse button) within the upper scale bar 11 , you can adjust the number of images which are shown by moving the mouse left or right. Minimum is 20 and maximum is half of the recorded images in this scale. Quick scrolling: you can quickly scroll through the thumbnails by dragging the orange bar with the mouse. While quick scrolling, the Preview window will display the active image sequence. This allows you to quickly scroll through the image sequence displaying the live images in the Preview window forwards or backwards. The View window will not actively show live images during quick scrolling (only in normal scrolling speed by mousewheel). If you click on a thumbnail image it will be shown on the view window. You can scroll via mouse wheel through the thumbnails. The upper blue bar correlates to the number of displayed thumbnails. The lower blue bar shows the range of the upper scale in relation to the whole record. 2 The second scale shows the total number of recorded images. It allows to scroll fast through the images 22 . 42 6 CAMWARE 4 SOFTWARE Right-click menu (click on thumbnails): Allows to rebuild all thumbnails and to search for events. Furthermore the Set In / Out gives you the possibility to set values for a sequence, which can be played via play button. Reset In / Out discards these settings. Set In / Out is active: if you save/export your images, only the selected images are saved/exported (see 6.9.2). 3 The light gray area 3 in the upper scale shows an In-Out example area. It is very easy to define a new area: just right-click on the start and end frame in one of the scales. The In image must be left to the red bar, the Out image to the right of the red bar. Search Events in Thumbnails: Detected events are displayed as green bars Too dark or bright thumbnails: if thumbnails are too dark or too bright, right-click in view window (see 6.9.8) and select Auto Range Peak or Auto Range Crop. Then right-click on a thumbnail image and select Rebuild Thumbnails. Now the thumbnail images should comply with the view window. Use your keyboard to scroll through the Images: Page up / down keys: 10 Images up or down Arrow keys: quick scrolling through the images. Advantage: fluent video playback in the View window (forwards or backwards). Home/Pos1 key: first image. End key: last image. 43 6.8 SETTINGS OVERVIEW 1 2 3 4 5 6 7 8 9 10 11 Settings overview shows the most important parameters of your camera(s) at a glance. If you have more than one camera connected, each camera and its parameters are listed. The parameters can only be changed under 6.3 Camera Properties. It is possible to easily switch between the Recorder (Images) section and the Settings Overview. 11 No. Function Camera name Auto Save Type Status 5 6 7 8 9 10 Frame rate Resolution Exposure time Number of images T0 Position Synchronization Status 1 2 3 4 44 Description Name Off , Unconfigured (red), OK (green) Camera type and serial number Ready or Recording Green background: Images are in memory Currently selected frame rate Resolution in pixels Selected exposure time Number of images to be recorded not available not available 6 CAMWARE 4 SOFTWARE 6.8.1 AUTO SAVE Auto Save helps to save your recorded images or sequences in an easy way. There is no need to save each image/sequence separately from each connected camera. Therefore this function is very useful if you use more than one camera. Once configured Auto Save allows acquiring and saving as many images/sequences as you will need during your experiment. This function can store RAW (e.g. TIFF) and Export (e.g. AVI, JPG) files. Standard file save see File Menu 6.9.2. Explanations will be displayed in the Info Text window at the bottom of the menu. Enable Auto Save by clicking on the check box. The text will change to Unconfigured! (red background). Right-click on the Uncofigured! field and click on Configure ‘Auto Save’.The Auto Save Options dialog will be displayed. First, configure the General Auto Save Settings - Global Auto Save Mode: two different modes are available, Save manually and Save unattendedly. The Save manually mode allows to store RAW images and export images after a recording session, when you hit the SHIFT and A keys. This gives you the possibility to cut the image sequence in the Recorder Toolbar before saving. The Save unattendedly mode enables to download all RAW images and to export the complete image sequences of all cameras immediately after an active recording is stopped. Select Output: Off – Auto Save is deactivated Save RAW – Only 16 bit RAW files are stored (b16, pcoraw, MultiTif-File, Tiff Export – only compressed files are stored (BMP, JPG, Tiff, AVI, MPEG, WMV) Save RAW and Export – RAW and compressed files are stored simultaneously Common Folder: main folder for your stored files RAW and Export file Type: select the type of RAW and Export file Export Color Image: select if you want to export color images (only for color cameras) Apply Automatic File Naming: if set to yes, your stored files are automatically named by Camware according to your automatic file name settings. 45 General Auto Save Settings – File name Set your file name individually by adding or deleting items. Position these elements as needed. Camera Specific Auto Save Settings: Configure camera connected camera. specific settings for each Save RAW File Settings: set RAW File Folder and RAW File Name (if not set to automatic file naming). Export File Settings: set Export File Folder and Export File Name (if not set to automatic file naming). Multimedia File Resolution: set predefined video export resolution or enter a Custom x- and y-resolution. Most likely you should set the applied resolution of your camera here. Finish the configuration by clicking OK. After configuration is finished, Auto Save status will be set to OK (green background). 46 6 CAMWARE 4 SOFTWARE 6.9 CAMWARE MENU TABS & FEATURES This chapter describes in detail the Camware Demo Mode and the Camware Tabs: File, Camera, Acquisition, View and Window. Furthermore the right-click menu and some additional features are listed. 6.9.1 DEMO MODE When Camware is started, it automatically recognizes the camera type of the connected and running cameras. Camware will start in Demo Mode, if your camera is switched off or no camera is connected In this mode all image processing features are available, but all camera settings are deactivated. The user only has to tell Camware what type of image he wants to open. For that purpose, the Demo Mode Setup window opens and asks for the corresponding input. Need Help? If this window pops up because you have trouble running the camera, please see instructions in appendix A8. Resolution The drop down list displays the existing image sensor spatial resolutions of all PCO camera systems. Please select the specific resolution and bit depth of the images to be opened. If double shutter images have been recorded and should be opened, Double Shutter Mode should be checked. Color With the radio buttons, the user can specify whether the image type is monochrome (b/w) or color. Alignment These two radio buttons adjust whether MSB (most significant bit) aligned (upper) or LSB (least significant bit) aligned (lower) images have been stored. Infotext The Infotext is automatically shown in Camware if you open a stored image sequence. The Camera Properties settings, storing location and Record date are listed in this file. Infotext can be activated in the View Menu 6.9.5 at any time. 47 6.9.2 FILE MENU Open RAW File (single image only) This command should be used to import a single image into the currently active image window. Only files with the extension and format of *.b16 (=PCO proprietary binary image format) and *.tif (16 bit TIFF image format) can be imported. If the recorder is enabled, each imported image will be transferred to the buffer shown in the picture number. The image itself will be fitted to the current image size. If the recorder is disabled, the current image sizes will be set to the parameters of the imported image. Open RAW Recorder Sequence (image sequence from one camera) This command is used to import a sequence of images. If more than one camera is connected and an image window is currently open, the sequence will be loaded to the active window. If no image window is open, the images will be loaded to camera #1. This command opens the Open file dialog box. Only files with the extension and the format of *.b16, *.pcoraw, *.tif and multi tif can be imported. NOTE Be aware of the different storage abilities of the formats, for example *.bmp - the bitmap format only allows for 8bit values to be stored and therefore the image content of a 16 bit image is reduced, if stored as bitmap. Save RAW File (single image only) This command should be used to save the image, which is displayed in the active window. The command opens the Save file dialog box. The image file can be saved in 16bit *.b16 and *.tif format. If more than one camera is connected, it is possible to save all current images by selecting Export all images in the Save file dialog box. With this feature it is possible to save one image of each active camera within one process step (it is not necessary to repeat the save process for each camera). The Save command will not be available, if no image window is open. For Auto File Save see 6.8.1 Save RAW Recorder Sequence (image sequence from one camera) This command should be used to save or export image sequences. If more than one camera is connected and an image window is currently open, the record of the active window will be saved. The command opens the Save recorder file dialog box. It is possible to select the number of saved images, to step images and to choose the first image number. For Auto File Save see 6.8.1 Export File (not reloadable!) Use this command to export the image of the active image window. This command will open the Export Image dialog box. Files with the extensions fts, tif, bmp, asc, jpg, and jp2 can be exported. This item will not be visible, if no image window is open. For Auto File Save see 6.8.1 48 6 CAMWARE 4 SOFTWARE Export Recorder Sequence (not reloadable!) Use this command to export a sequence of images. If more than one camera is connected and an image window is currently open, the record of the window which has got the input focus will be saved. If no image window is open the Export Recorder Sequence menu does not appear. This command will open the Export recorder box. Files with the extensions fts, tif, bmp, asc, avi, mpg, jpg, jp2, and wmv can be exported (see Appendix A7). Auto File Save see 6.8.1 Options Single File Properties Single tif file 16 bit alignment: upper / lower ASCII File Separator: select a separator for the values in the ASCII file. Select: tab, space, semicolon, colon, comma, hyphen, slash, backslash. Binary pgm file: set the format of the pgm (portable gray map) file. Select: yes, no. Jpeg2000 image quality: set compression from 20 to 100%. Jpeg image quality: set compression from 20 to 100%. Binary ppm file: set format of the ppm (portable pixmap) file. Select: yes, no RAW 16bit RGB tif file: save raw tif without color balance. Select: yes, no. Use cache file: Caches image data on disc for a camera with camera internal memory. Select: yes, no General File Properties FIFO buffer size: set the FIFO buffer size in number of images. This helps to avoid gaps during file write delays. Usually this is set to 150. Preserve last record: preserve current recorded images. When set, the user will be asked whether to really start a new record or to close. View Properties: Crosshair color: set the color for crosshair, save ROI and line tool. Crosshair length: set the crosshair length in pixel. Activate crosshair: see chapter 6.9.8 49 Open AVI Codec Dialog You only need to set these options, if you use Auto File Save see 6.8.1 Please select the (compression) codec that you want to use for your stored sequences. All installed codecs are listed here. If you use Auto File Save, this setting takes an effect on your stored video sequences. Load lookup Table (for monochrome cameras) With the Pseudo LUT (Lookup-Table) feature you can load any LUT with one of four different formats and you can view the result in the color view window. Use one of the attached predefined LUTs or define your own. Direct Record to File With this command you can preset a certain number of images to be stored. If the camera captures images faster than the computer can save to disk, then you will lose images. The displaying of the images doesn't interfere with the record process. Start Auto Save Only available if Auto Save is activated (see 6.8.1) Exit This command exits the program and closes all channel dialog windows. Window positions, settings and sizes are stored in the windows registry and will be loaded again at next start-up. 6.9.3 CAMERA MENU Camera Control Use this command to open the camera control window. Close Disconnects camera and switches Camware to Demo Mode. In case of multiple cameras, all cameras must be closed in order for Camware to switch to Demo Mode. Rescan Disconnects and reconnects all cameras. 50 6 CAMWARE 4 SOFTWARE Setup Switch between Rolling Shutter, Global Shutter and Global Reset Mode. The camera will automatically restart. A B C Five different readout modes are available in Rolling Shutter (6.3.2). Standard mode is Dual Outside in. In Single Top down, the pco.edge provides only half of the normal frame rate. Camware rotates the image that was recorded by the sensor, by 180°. This means, that the last line of the image is the first line of the sensor. PCO SDK names are from A to E. D E A B C D E 6.9.4 ACQUISITION MENU Live Preview The Live Preview is useful for fast and easy adjusting and focusing of the camera. The active window will be updated. To see another window, simply click on the window. This option is not available in double shutter mode. Acquire Picture (not available) Acquire Sequence Starts recording images into the system memory according to Trigger Mode selection (see 6.3.1). During recording, all camera controls are locked. Rec. Memory Settings This sets the number of images recorded in one sequence. The maximum is defined by approved RAM size. 51 6.9.5 VIEW MENU B/W or Color Window Use this command to open a new display window. Convert Control See chapter 6.3.9 Toolbars and Docking Windows Standard toolbars of Camware 4 are Recorder/ Recorder Tools / Camera Overview / Camera Properties and Image Overlay. Additional Toolbars known from Camware 3.x are displayable, but not essentially needed: Main Toolbar / Extended Recorder / Cursor. For function Infotext see 6.9.1. Application Look The Style and Look of Camware can be customized; many different style sheets are selectable. The Tabbed MDI function (un)docks the view windows. Reset layout to default This resets all your customized changes and restores the default layout. 6.9.6 WINDOW MENU New Window A new view window will be opened. Close Active Window Active window will be closed. Split The view window will be split in four quarters. Camera overview Shows all connected cameras. 52 6 CAMWARE 4 SOFTWARE 6.9.7 HELP MENU Contents Opens the main page of the program help. Search for Help on Opens an index list for help. Create Support File This will activate the Camware log files. Press YES to activate log files and reboot Camware and your pco.edge. After log files are activated it is possible to create a support file. Please send this file to the PCO support (see A8.4). Logging Enable Logging: Activates Camware log files (this cuts down performance) Clear Logfiles: (only visible if logging is enabled) this command erases all actual log files Explore Logfiles: opens windows explorer Disable Logging (only visible if logging is enabled) disables logging Support Mail This command opens your email-program and an already created support file will be added automatically as file attachment. About Camware This window shows program information. 53 6.9.8 RIGHT-CLICK MENU NOTE Right-click in the View window to open this menu. View Color: color window. View Window B: if Double Shutter mode is activated, this will switch to window B (second image). Stretched View: image will be fitted into the display window. Stretched View Ratio: aspect ratio are maintained. Zoom +/-: image zoom (only available if Stretched View is deactivated). Zoom set the factor of the Zoom (from 0.0625 to 32). Scroll Synchronous if more than one image window is opened, it is possible to scroll synchronously through the images (only available if Stretched View is deactivated). Show Image Overlay: activates the overlay see 6.4 Open LUT: opens look-up table file for false-color illustration. Auto Range Peak searches for the minimum and maximum 14 bit intensity values of the image. Given these numbers the converter scales the 8 bit display (256) within these two values. Auto Range Crop sets the converter to ignore the extreme intensity values of the image and scales the display in a smaller range. Thus dark or bright light spots, reflections, etc. are cut off. Continuous Auto Range (Crop): This option enables the automatic min/max function during record and replay. Flip/Mirror: image will be flipped or mirrored. Rotate Left/Right: rotates the image. Set ‘File Save ROI’: if you want to save just a part of the recorded image (region of interest), it is possible to draw a rectangle with the mouse. This rectangle is valid for all recorded images and can be dragged at its edges. 54 6 CAMWARE 4 SOFTWARE Line Tool: Select Line Tool to show a graph, which shows the intensity of the pixels under the red line shown in the image window. The selection of this menu item determines the location of the first point. After this you can move the mouse to the second point, which can be selected by a left click. Now a Line Diagram opens and the length of the line (unit: pixel) is displayed in the window. Copy to Clipboard: will copy the actual image to clipboard Crosshair: activates a centered crosshair. Size and color are selectable see chapter 6.9.2 → Options. Crosshair is movable, just drag it by mouse. Reset it to center position by double-click into center of the crosshair. 55 6.9.9 ADDITIONAL FEATURES White Balance by Mouse It is possible to change white balance easily by mouse: You only have to press the CTRL (Strg) and the shift button at the same time and select a white or gray area within the image. The pixel values within the coordinates of the selection rectangle are used for calculating a new white balance. For best results we recommend to use the white balance button in the Convert Control Color (see 6.3.9). Fold Up Window The Convert Control windows can be minimized / folded up 1 . Just move the pointer over the bar and the window will unfold again 2 . 1 2 Setting Contrast Area by Mouse You can control the minimum and maximum values used for the conversion from 16 bit to 8 bit with the mouse. Move the mouse cursor into a region which should be shown with maximum contrast. Press the shift and the left mouse button. Hold down the mouse button while increasing the size of the selection rectangle with mouse moves. After releasing the mouse button the coordinates of the selection rectangle act as a border for calculating the minimum and maximum values. Setting a new ROI by Mouse In the same manner you can setup a new region of interest (see 6.3.5 ROI) for the camera. You only have to press the CTRL (Strg) button and drag an area with the left mouse button. The coordinates of the selection rectangle are used for calculating a new region of interest, which will be adapted to the camera capabilities automatically. You can reset the ROI to maximum by pressing the CTRL (Strg) button and the right mouse button. Short Cut List • Acquire Picture: SPACE • Acquire Sequence: STRG + A • Auto Save: SHIFT + A • Export File (Image): STRG + T • Export Recorder Sequence (Video) STRG + O • Open Raw Image File: STRG + I • Open Raw Recorder (Video) Sequence: STRG + R • Save Raw Image File STRG + E • Save Raw Recorder Sequence (Video) STRG + S 56 APPENDIX A1 TECHNICAL DATA 58 A1.1 STANDARD VERSION 58 A1.2 WATER COOLED STANDARD VERSION 58 A1.3 CAMERA LINK HS VERSION 59 A1.4 SPECIFICATIONS 60 A1.5 MOUNTING A1.5.1 INSTABLE MOUNTING A1.5.2 CORRECT MOUNTING A1.5.3 COOLING 60 60 61 61 A1.6 REVERSE WITH CAPTION 62 A2 CHANGE FROM F-MOUNT TO C-MOUNT 63 A3 WATER COOLING OPTION PCO.AQUAMATIC II 64 A3.1 SYSTEM COMPONENTS 64 A3.2 FIRST TIME INSTALLATION 65 A3.3 OPERATION 66 A3.4 DIMENSIONS 67 A3.5 YOUR OWN COOLING SYSTEM 67 A4 INTERFACES 68 A4.1 CABLE POSSIBILITIES 68 A4.2 CAMERA LINK A4.2.1 INSTALLATION OF FRAME GRABBER A4.2.2 MICRO DIAGNOSTICS TOOL A4.2.3 IMAGE DATA FLOW 69 69 70 71 A4.3 CAMERA LINK HS 73 A4.4 USB 3.0 A4.4.1 DRIVER INSTALLATION A4.4.2 HARDWARE RECOMMENDATIONS A4.4.3 USB 3.0 FAQ A4.4.4 INSTALLATION OF THE USB 3.0 CARD A4.4.5 RING BUFFER AND FIFO 74 74 74 76 78 79 A5 BINNING WITH CMOS SENSORS 80 A6 LIGHTSHEET SCANNING MODE 81 A7 IMAGE FILE FORMATS 82 A8 CUSTOMER SERVICE 84 A8.1 SERVICE 84 A8.2 MAINTENANCE 84 A8.3 RECYCLING 84 A8.4 TROUBLE SHOOTING 85 A9 INDEX 86 ABOUT PCO 87 57 A1 TECHNICAL DATA A1.1 STANDARD VERSION (pco.edge 3.1/ 4.2 (LT) / 5.5: Camera Link or USB 3.0) A1.2 WATER COOLED STANDARD VERSION (pco.edge 4.2 / 5.5 Camera Link) 58 A1 TECHNICAL DATA Water Cooled Standard Version (pco.edge 4.2 / 5.5, USB 3.0) The housing size is similar to the dimensions of the standard version, except the camera design with the USB 3.0 interface allows locating the water connections on the back of the camera. A1.3 CAMERA LINK HS VERSION (pco.edge 4.2 / 5.5 Camera Link HS) 59 A1.4 SPECIFICATIONS General power supply power consumption weight operating temperature operating humidity range storage temperature range optical interface CE / FCC certified 12 .. 24 VDC (+/- 10 %) 21 W max. (typ. 11 W @ 20 °C) 700 g (CL) / 930 g (USB) + 10 °C .. + 40 °C 10 % .. 80 % (non-condensing) - 10 °C .. + 60 °C F-mount & C-mount yes Sensor type of sensor dynamic range image sensor resolution (h x v) pixel size (h x v) sensor format / diagonal fullwell capacity (typ.) spectral range scientific CMOS (sCMOS) 16 bit CIS2020 CIS 2521 2048 x 2048 active 2560 x 2160 active pixel / pixel 6.5 µm x 6.5 µm 6.5 µm x 6.5 µm 13.3 mm x 13.3 mm / 16.6 mm x 14.0 mm / 18.8 mm 21.8 mm 30 000 e30 000 e370 nm .. 1100 nm 370 nm .. 1100 nm A1.5 MOUNTING A1.5.1 INSTABLE MOUNTING These instructions are not valid for every application. Especially in microscopy when extreme magnification is used, the smallest vibration can disturb images. The pco.edge needs a stable mounting plate to avoid disturbing vibrations and to ensure a consistent high image quality. (images are mounting proposals). Don’t use such an instable mounting plate! 60 A1 TECHNICAL DATA A1.5.2 CORRECT MOUNTING Recommended mounting. The mounting plate must have a centred opening, as the cooling fan is located on the bottom of the pco.edge. The fan and its louvers must never be blocked. A stable support for the rear area of the camera is very important. Attach your pco.edge camera with three ¼ - 20 UNC Allen screws. Caution: • • • Mount the pco.edge on a stable plate Use all three mounting threads Never block the fan and the louvers A1.5.3 COOLING These images show the ventilation flow of the pco.edge camera. The fan and the air intake are at the bottom of the camera. The air outlets are on top and on both sides of pco.edge. The camera needs a sufficient supply of fresh air to reach a constant operating temperature. Caution: • Never block the fan and the louvers • Keep adequate distance to other components • Provide a sufficient supply of fresh air • Mounting plate needs a cut-out for cooling 61 A1.6 REVERSE WITH CAPTION Specification of the reverse: 4 3 1 5 2 SMA-Jack 1 50 Ohm Amphenol 901-143-6RFX See Chapter 6.3.8 Pin1 Pin2 LEMO socket: 2 ECG.0B.302.CLV Pin1: Ground; Pin2: VCC Appropiate Lemo plug: FGG.0B.302.CLAD52Z Status LED 3 Red blinking: Green continuous: Green blinking: Yellow blinking: error camera is booting camera is ready for operation recording on Power switch: 4 on / off 5 Interface connector: 5 Depending on your interface: USB 3.0, CameraLink or CameraLink HS. Water connectors: 6 Colder Product Company SM212 ¼ hose connector 5 6 62 A2 CHANGE FROM F-MOUNT TO C-MOUNT A2 CHANGE FROM F-MOUNT TO C-MOUNT Instructions on how to change the optical input from F-mount to Cmount: Step 1: Remove F-mount Adapter Grasp the F-mount adapter at the blue ring (counter ring) and turn it counter clockwise. F-mount adapter Step 2: Insert C-mount Ring Carefully screw the ring completely in and tighten the two Allen screws. C-mount ring Adjust Back Focal Length First of all, attach a lens to your c-mount adapter. Then set the focus of your lens to infinity. After that, look for a point in infinity* and generate a sharp image by turning the smaller ring of the adapter. Then fix this position with the two small indented screws. *rule of thumb: object should be away about 2000 times the focal length in mm Allen screws Limitations of C-mount lenses Keep in mind that c-mount lenses could cause shadings at the edges of big sized sensors. Most c-mount lenses are able to illuminate a maximum image circle of 11 mm (2/3”), 16 mm (1”) or 22 mm (4/3”) diameter only. The pco.edge cameras have a sensor diagonal from 18.8 to 21.8 mm, it follows that you have to use the ROI function for a shade less image while using the cmount adapter with the two smaller C-mount diameters. 63 A3 WATER COOLING OPTION PCO.AQUAMATIC II This is the re-cooling system for pco.edge cameras with watercooling. A3.1 SYSTEM COMPONENTS 1 2 Description 3 5 4 1 pco.aquamatic II 2 Connection Tube 5m PVC 3541-01 PCO (with Colder NS212 fittings) 3 Power supply 1.2m 4 Power cable 5 EDGE WAT camera cable 5m FGG-RG58- NC3MX 6 Innovatec Protect IP 1L The operation of the pco.aquamatic is simple and uncomplicated. Normally no maintenance and nearly no attention are needed. Only the liquid level of the reservoir (water tank) should be controlled from time to time. 6 Only use Innovatec Protect IP for the pco.aquamatic! Do not use or add any other cooling liquid or normal water! If you need to add cooling liquid in order to maintain level in the tank, please contact PCO for additional supply. The cooling liquid will turn yellow after some hours of operation. This is normal and no sign of wear or malfunction. The optimum pH-value is between 8 and 9 (please check this value if you are concerned about the cooling liquid quality). NOTE The recommended service interval for the change of the cooling liquid is four years. 64 A3 WATER COOLING OPTION PCO.AQUAMATIC II A3.2 FIRST TIME INSTALLATION 3 5 4 2 Please take care to situate the unit on a flat and firm surface. Do not cover the air intakes of the unit. Please ensure free airflow around the pco.aquamatic to ensure maximum cooling performance. All tubes and power cords need to run kink-free. Before installation of the unit carefully read the Innovatek Protect IP safety datasheet (see Innovatek Website). outlet flow inlet flow 1 6 Please follow steps 1 – 6. •1 Connect tubes to cooling unit and camera. The two arrows on the housing of the cooling unit only indicate flow direction. Either connection of the camera can be used for in or out. •2 Attach power connection. •3 Open tank cover. •4 Turn power switch to on position (I). •5 Slowly fill in the cooling application mixture while the unit is running, you have to constantly refill liquid. •6 While the cooling liquid flows back to the reservoir make sure that the whole air escapes from the system – this takes a few minutes (move hoses if necessary). The cooling liquid reservoir (tank) is filled when liquid level is approximately 1-2 cm from the top of the tank. The integrated pump only works when the pump chamber is completely filled. To ensure this please move hoses or remove air by evacuating. Reservoir capacity is approximately 500 ml. After steps 1 – 6 are completed successfully the system is ready for operation. NOTE The hose connectors are waterproof in not connected state. Maybe they lose one drop of cooling liquid from time to time. You don’t have to empty the hoses while storing the camera system. 65 A3.3 OPERATION First connect the power out of the cooling unit with the power in of the pco.edge camera by using the PCO WAT camera cable. The cooling unit provides two operation modes. Operation Mode on: the cooling unit is turned on permanently and provides the camera with power. Your camera can be switchted on and off as needed. Operation mode follow: the cooling unit will turn on when the camera is switched on and vice versa. Error Codes The coolant temperature sensor is located in the water tank. Temperature 27°C 36°C 55°C 60°C Action fan turns off fan turns on warning message error message If a warning level is passed, the Power LED blinks slowly and the Error LED displays the error code. If a failure level is passed, the Power LED blinks fast and the Error LED shows the error code. Error / Failure on 1Hz flash 2Hz flash 1Hz flash off one short flash one short flash two short flashes none Warning when temperature at 55 °C (also if sensor is defect or missing) Failure when temperature at 60 °C Fan speed (also if a high deviation of the standard value is reached) NOTE The camera has its own protection circuit and will shut down Peltier cooling automatically when the electronics temperature exceeds safety level. The camera itself will keep on running, but sensor temperature will increase. (valid for air and water cooling!) If the camera and the water cooler have a different power supply, always first shut down camera and then the cooling system to avoid damages. 66 A3 WATER COOLING OPTION PCO.AQUAMATIC II A3.4 DIMENSIONS All dimensions are given in millimeter. Weight: 4kg (completely filled cooling liquid tank) A3.5 YOUR OWN COOLING SYSTEM You are not restricted to purchase the pco.aquamatic system - it is possible to use an own water cooling solution. A separate power supply will be provided to every pco.edge camera with water connectors. The hardware of the pco.edge cameras with USB 3.0 Interface is designed to work with or without a water cooling system, because a fan that provides adequate cooling is inside the camera anyway. In case you use an own water cooling system, please make sure that the liquid you use to cool your camera is at all times never below the dew point of the ambient temperature. In order to avoid any appearance of condensation, use a cooling liquid at room temperature. • • A liquid flow rate of 1–2 litres per minute is sufficient Always use Innovatec Protect IP cooling fluid or an equivalent cooling fluid 67 A4 INTERFACES Three different interfaces are available for the pco.edge camera. This chapter describes the installation and configuration of USB 3.0, Camera Link and Camera Link HS interfaces. Furthermore USB range extension, Ring Buffer and FIFO Buffer are described in detail. A4.1 CABLE POSSIBILITIES Overview of the maximum cable lengths Camera Link full: • standard: 3m • optional: 5m • maximum: 10 m (active cable) • no cables are included in the camera price NOTE Please only use Camera Link cables, which have been are approved by PCO. Otherwise full functionality is not guaranteed. Camera Link HS • FOL fiber optic cable: length up to 300 m with SFP+ connector USB 3.0: • standard: 3 m • cable and proper PCI express board included in the camera price 68 A4 INTERFACES A4.2 CAMERA LINK A4.2.1 INSTALLATION OF FRAME GRABBER Instructions for installing and testing the Silicon Software microEnable IV (ME4) Camera Link grabber card. This card is required to be able to use a pco.edge with Camera Link interface. Installation must be performed by a technician, because high voltages can occur on the device. WARNING ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE Risk of injury due to electric shock. Always pull the main plug before opening the computer. Please install the latest silicon software runtime package before installing the hardware. (Download: http://www.pco.de/support/) When working on a 64 bit operating system, please make sure to install the proper (64 bit) runtime when also a 64 bit application will be operated. If the application is 32 bit, you need to install the 32 bit runtime accordingly. •1 •2 •3 • 4 •5 Select full installation Let the program also update device drivers. Shutdown your computer, open the computer case and install the grabber card The grabber card should be displayed within the device manager. If the device is not shown this way, please reinstall the SiliconSoftware device driver. After the installation, please start the program microDiagnostics. (see next page) 2 1 3 5 4 69 A4.2.2 MICRO DIAGNOSTICS TOOL 1 Micro Diagnostics Tool works with both meIV (AD4/VD4 for CL) and meV (CLHS) frame grabber cards. To test the board, select the board in Diagnosis and click Board 1 to start the test 2 Please upgrade to the supplied firmware. Select Tools → Flash Board(s) 3 and select the appropriate hap file. Micro diagnostics provides the latest available firmware version of the installed runtime. Then click on Yes when you’re asked to proceed. You must restart your computer after the firmware upgrade. Please test the performance of your frame grabber card: click on Performance 4 to start the test. 4 It is mandatory that PCIe is Highspeed Capable 5 is shown. Otherwise the board is probably not able to transfer the necessary data rate. 5 For further information or problems with mainboards please contact our support section. 70 A4 INTERFACES A4.2.3 IMAGE DATA FLOW The sCMOS image sensors provide an extremely high frame rate compared to other scientific image sensors. The high frame rate, along with the high dynamic, creates a large amount of data that must be handled and stored. The maximum data rate of the sCMOS image sensor is given by: pco.edge 5.5 Camera Link: [2560 * 2160 (pixel in 1 frame) * 2 Byte (= 16bit dynamic)] * 100 [frames/s] = 1.1 GB/s (pco.edge 5.5, Camera Link) To handle this considerable amount of data, there are two options available: Option 1: Real-time Recording to Computer The pco.edge sCMOS camera series uses this option. For this purpose, the interface must be capable of transmitting data at the required speed. Interfaces such as GigE, USB 3.0 and Camera Link are not fast enough to transmit this data, which is delivered by the largest sCMOS image sensor. Nevertheless, there is a sophisticated solution that uses the Camera Link interface, which is integrated into the pco.edge camera. It is a fact of nature that light, itself, has its own noise component called photon or shot noise, which increases with light signal. In this approach, no compression is made in small signals, while for large signals a suitable compression is applied. Since the introduced compression error is always smaller than the photon noise induced error, it is not seen and a so called visual lossless compression has been performed. It can be shown that this is possible without any significant loss of information. Therefore, the calculation for the pco.edge camera at full speed and full frame has to be re-written: [2560 * 2160 (pixel in 1 frame) * 1.5 Byte (= 12bit dynamic)] * 100 [frames/s] = 0.829 GB/s The Camera Link interface can transmit this in real-time. In the future, recently introduced machine vision interfaces including CoaXPress and Camera Link HS are capable of transmitting sCMOS image data without any compression. A network type interface, 10GigE, is similar with respect to hardware to Camera Link HS, but incorporates all of the known GigE advantages and disadvantages. Here, the usual protocols are not favorable for image data transmission, and any network traffic can dramatically reduce available transmission speed. The real-time data transmission into the computer allows for a variety of applications, since it is free from camera memory limitations. Image data can be stored directly in the computer’s random access memory (RAM) up to more than 64 gigabyte. With an appropriate RAID system, the data can be stored directly to hard disks and there is no delay involved. Option 2: Recording in the Camera For high-speed imaging applications where data transfer rates are in the range of several GB/s it is accepted that the primary image memory (camRAM) must be located in the camera. Two examples of 71 such high-speed cameras are the pco.dimax with 36 GB of camRAM and the pco.dimax HD with 18 GB of camRAM memory. This allows for fast recording, but just up to the integrated memory limit. Before a second sequence can be recorded, one must endure the wait time until data is downloaded to computer storage. Therefore, this option can only be used for recording short sequences with enough time between each event to download the image data. This option is not necessary for the pco.edge as either with USB 3.0 or Camera Link interface, the image data will be recorded to the computer in realtime. Memory Structure / Organization As the memory is software-controlled in both options, it does not matter how the data is stored. The memory can be organized for ring buffer or FIFO recording, or for a specified number of images like a burst mode. The pco.edge camera enables all of these possibilities with the integrated dynamic link libraries and proprietary Camware application software. This allows the customer to select the memory structure and organization that is optimized for the application at hand. Recommendations In the following you can find two exemplary systems for RAID and RAM storage options that have been successfully tested by pco for high performance and which we sell directly to our customers. They only show a configuration example, for actual system configuration please call us directly. RAID 0 SYSTEM RAM RECORD ER NOTICE 4x SSD SATA 6 GB/s 16 GB DDR3 RAM Intel® Core™ i7 GeForce (NVIDIA CUDA) Windows 7 Professional 64 bit 64 GB DDR3 RAM Intel® Core™ i7 GeForce (NVIDIA CUDA) Windows 7 Professional 64 bit Items are exemplary and subject to change. Please contact PCO for recommendation of current systems and sale of these systems. The use of normal consumer PCs is not recommended! ASUS mainboards seem to fail generally during the HS-mode test and are not recommended. Recommended minimum configuration (or better): • • • CPU >= i7 2.5 GHz RAM >= 1066MHz, 8GB Mainboard ≥ Supermicro X9SRA (socket 2011) 72 A4 INTERFACES A4.3 CAMERA LINK HS CLASS 1 LASER PRODUCT Risk of injury due to laser beam. Do not look into the laser beam or at direct reflexes. Do not point the laser beam at persons. Manipulations of the laser device are not allowed. WARNING ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE Risk of injury due to electric shock. Always pull the main plug before opening the computer. Your pco.edge CLHS is delivered with a Silicon Software microEnable V marathon AF2 frame grabber card. The installation and configuration of the frame grabber card is identical to the CameraLink mE IV card: see A4.2.1 Minimum system requirements: • PCI Express x4 (Gen 2), DMA1800 • DDR3 Ram ≥ 8GB Short installation instructions: • Install frame grabber card to your computer • Install driver of your frame grabber card • Connect your pco.edge CLHS to frame grabber card • Start your camera • Run Camware Camera Link HS specifications: • more bandwidth - up to 1187 MB/s • more robust connection - no communication error with Forward Error Correction (FEC) and Fiber Optic Link (FOL) • more distance - 300 m with multi mode fiber and SFP+ connectors • more open - full CLHS specification is downloadable for free • more cost effective - use of standard network hardware components allows multi sourcing 73 A4.4 USB 3.0 A4.4.1 DRIVER INSTALLATION 1 Install PCO USB 3.0 Driver For pco.edge USB 3.0 you always need to install the latest USB Driver version. After these two screens 1 2 the driver is completely installed. 2 A4.4.2 HARDWARE RECOMMENDATIONS The pco USB 3.0 interface is based on the Cypress EZ-USB FX31 device and it is compatible to pco software such as Camware and SDK. To run a pco USB 3.0 camera successfully the user should consider a number of important issues that are discussed in the following chapters. Motherboard and Chipset Configuration It is recommended to use a Motherboard with a state of the art USB 3.0 host controller for the onboard USB 3.0 ports. The latest generation of USB 3.0 xHCI host controllers manufactured by Intel, Renesas (NEC), Fresco, Via Labs, ASMedia and Texas Instruments Inc. (TI) are tested by Cypress Semiconductor Corporation. (see Cypress EZ-USB® FX3TM SDK Release Notes, Version 1.2.3, chapter 1.3). Currently pco recommends only motherboards with the following onboard xHCI host controllers: • Renesas/NEC μPD720202 Host Controller • Intel® USB 3.0 eXtensible Host Controller If the motherboard does not have an onboard USB 3.0 port or the onboard USB 3.0 port does not work properly with a pco USB 3.0 camera, please use an independent PCIe USB 3.0 extension card. In this case, pco recommends the following: • DELOCK 89348 (U3-PCIE1XG202-10) PCIe x1 Gen/V 2.0 (μPD720202 host controller) (This board is sent with your pco.edge USB 3.0 camera system as standard) • DELOCK 89325 (U3X4-PCIE4XE101) PCIe x4 Gen/V 2.0 (μPD720202 host controller) 74 A4 INTERFACES USB 3.0 extension card with xHCI4 host controller The following companies manufacture USB 3.0 xHCI host controller: • Renesas Electronics America Inc. (earlier NEC), • Texas Instrument Inc., • VIA Labs Inc (Diamond), • Fresco Logic Inc., • Intel Inc., • AMD Inc. and • Etron Technology, Inc. NOTICE NOTICE To determine suitable manufacturers of the xHCI controller, please use the Windows Device Manager. For example, in Windows 7 drop down the USB Controller entry and find the USB 3.0 host xHCI controller of the USB 3.0 device in the sub-tree. Then right-click to open the properties dialog, click the details tab, and choose Vendor in the properties pull-down box. USB 3.0 interface cards with controllers from Etron Technology, Inc. have not yet been tested. The following table gives an overview about tested recommended system configurations or hardware components. Component Motherboard USB 3.0 extension card PCIe Slot USB 3.0 Cable USB 3.0 Hub Operating System Multiple pco USB 3.0 cameras connected to a PC and Recommendation There is no recommendation for a manufacturer. But if an onboard USB 3.0 port is used, the Renesas´s xHCI host controller μPD720202 is recommended. • DELOCK 89348 (U3-PCIE1XG202-10) PCIe x1 V2.0 (μPD720202 host controller), • DELOCK 89325 (U3X4-PCIE4XE101) PCIe x4 V2.0 (μPD720202 host controller) A PCIe 4x, 8x or 16x slot is recommended. USB 3.0 cable included in the packaging of your pco USB 3.0 camera Not recommended Win 7 / 8 / 10 32bit & 64bit A separate DELOCK 89348 (U3-PCIE1XG202-10) extension card for each camera should be used to guaranty maximum data throughput of each camera. Recommended Software Components The pco USB 3.0 driver supports Windows 7/8 (x86 or x64) and Windows XP x86 operating systems. Microsoft does not support USB 3.0 natively for Win 7 or older Windows versions. Therefore use the manufacturers’ supplied xHCI host controller driver. By contrast, in Windows 8 Microsoft does provide a native generic xHCI Driver but pco recommends using the driver from the corresponding manufacturer of the xHCI host controller. Currently there are seven different vendors producing USB 3.0 host controllers: 75 • • • Renesas Electronics America Inc. (earlier NEC) Texas Instrument Inc. Intel Inc. • • • • VIA Labs Inc (Diamond), Fresco Logic Inc. AMD Inc. Etron Technology, Inc. Please make sure that the most recent driver from the manufacturer is installed before a pco USB 3.0 camera is connected to a USB 3.0 port of your computer. The appropriate driver for the USB 3.0 port could be loaded from the website of the manufacturer of the USB 3.0 host xHCI controller; or from the website of the USB 3.0 extension card vendor. The current driver of the extension card included in the packaging of your pco USB 3.0 camera is added to the pco.USB stick delivered with each pco.edge. NOTICE To determine the driver currently in use for the xHCI controller, use the Windows Device Manager. For example in Win 7, drop down the USB-Controller entry and find the USB 3.0 host xHCI controller of the USB 3.0 device in the sub-tree. Then right-click to open the Properties dialog, click the driver tab and choose driver details Before the pco.edge USB 3.0 camera is connected to the computer the pco USB 3.0 Driver has to be installed. (see 4.1) Please visit the support section of our website to get the latest USB 3.0 camera driver. A4.4.3 USB 3.0 FAQ Can I use USB 2.0 PC components? If the USB Port, the USB Cable, or the USB Hub does not support USB 3.0, a USB 3.0 PCO camera will not operate properly. The camera will be recognized in the Device Manager as a USB 2.0 device. Operation of the PCO camera using Camware or one of the PCO SDK camera related functions will fail and the following message box will appear: “Please make sure your camera is connected to a USB 3.0 port. If your camera is connected to a USB 3.0 port, please read the application note APL_USB3_xxx.pdf”. How to identify the manufacturer of the USB 3.0 host controller? If a driver for the onboard USB3.0 port or an independent PCIe USB 3.0 interface card is already installed, open the Windows Device Manager. Then extend the ‘USB-Controller’ entry and find the USB 3.0 host controller of the specific USB 3.0 device in the sub-tree. Right click to open the Properties dialog and then click the ‘details’ tab which will allow you to identify the correct manufacturer of the USB 3.0 controller. Why can’t the PCO USB 3.0 camera be opened with Camware or the PCO SDK? In case a PCO USB 3.0 camera is connected to your PC and the Camware software starts in Demo Mode; or, the Camera Open Function of the PCO SDK returns an error, the explanation could be: The PCO USB 3.0 driver is not installed to your system and the PCO USB 3.0 device is listed as “unknown device” in the Device Manager. 76 A4 INTERFACES If so, right click the unknown device to open the properties dialog and install the PCO USB 3.0 driver. The driver can be downloaded from our website at http://www.pco.de/drivers/. If the PCO USB 3.0 driver is properly installed, there will be a PCO cameras entry with a ‘pco.camera with USB 3.0’ device listed in the Device Manager. –– Another issue could be that the USB 3.0 camera is not recognized by the OS and it does not appear in the Device Manger, meaning it is even not shown as ‘unknown device’. In this case please refer to Chapter 4.1 and follow the instructions provided. Why was the PCO USB 3.0 camera not recognized by the OS? There could be a problem with the USB 3.0 xHCI host controller of the Motherboard or with the extension card installed in the PC. Additionally, a bad USB 3.0 cable could be the problem. How to increase the performance of the USB 3.0 data transfer? To make sure the full data-throughput of the camera can be transferred to the PC’s RAM, it is recommended to use a PCIe 4x, 8x or 16x slot in the PC. In the case of using Windows 8, poor performance could be caused by the Microsoft`s automatically installed generic xHCI USB 3.0 driver. In this case please load and install the driver of the manufacturer`s xHCI host controller. If more than one PCO USB 3.0 camera is operated on a single PC, please note that extension cards have maximum bandwidths that are exceeded with two or more cameras. There exists a maximum bandwidth of an extension card, e.g. if the DELOCK 89348 (U3-PCIE1XG202-10) PCIe 1x card is connected to a PCIe 16x slot of the PC the effective bandwidth is about 360 MB/s. If two PCO USB 3.0 cameras are connected to both ports of the extension card, the accumulated data throughput of both cameras cannot exceed this 360 MB/s maximum. In this case it would help to use a second DELOCK 89348 (U3-PCIE1XG202-10) PCIe 1x card connected to a second PCIe 16x slot of the PC for the second PCO USB 3.0 camera. How many PCO USB 3.0 cameras can be connected to and operated with one PC? How many PCO USB 3.0 cameras are supported by the PCO USB 3.0 driver? The PCO USB 3.0 driver supports up to eight cameras using one PC The USB 3.0 PCO Driver Installer does not work. The installer will return a warning that the current OS is not supported. The installer supports the 32-bit and 64-bit versions of Windows 7 and Windows 8. If the installer file is executed and a no dialog window appears: Please try to install the driver manually using the Windows Device Manager. Contact our support team to get the driver files for manual installation. The driver is correctly installed, but the USB 3.0 PCO camera does not appear in the Device Manager: Make sure the PCO USB 3.0 camera is ready, the power switch is turned on, and the camera is connected to the PC. Note that while installing the camera to the PC that during the USB 3.0 driver installation it could take a minute or more until the PCO USB 3.0 device recognized and is available for use. 77 A4.4.4 INSTALLATION OF THE USB 3.0 CARD An external USB 3.0 Host Controller Card is enclosed to each pco.edge USB 3.0 camera. Hardware Installation First shut down your computer and install the USB 3.0 Host Controller. Hardware Installation must be performed by a technician, because high voltages can occur on the device. WARNING ELECTRIC SHOCK WARNING DUE TO VOLTAGE PARTS INSIDE Risk of injury due to electrical shock. Always pull the main plug before opening the computer. Driver Installation Instructions • Within the provided installation files USB_HBA, open the folder U3X4-PCIE4XE101, U3X4-PCIE1XE101, U3-PCIE1XG202. • Open the subfolder Driver and run RENESAS-USB3-Host-Driver30230-setup.exe. • If your current OS is Win7/8 and the User Account Control is enabled, a dialog could occur asking, if you wish to launch the setup: Accept with Yes. •1 At first the installation is prepared. •2 Secondly the software components are copied automatically. •3 Finally the installation is completed and the Delock USB 3.0 extension card can be used. 1 2 3 78 A4 INTERFACES A4.4.5 RING BUFFER AND FIFO Compared to the pco.edge cameras with Camera Link interface, the pco.edge with USB 3.0 interface has a small internal buffer memory integrated. Each recorded and read out image is directly transferred to this internal buffer and subsequently the image is transmitted to the computer, where it is either stored into the RAM or onto the hard disk drive. There are two transfer modes available: Ring Buffer and FIFO buffer which can be accessed via SDK function: PCO_SetStorageMode (see SDK manual). From user perspective the main difference between both modes occurs, if for some reasons the USB 3.0 interface behaves slower than its usual speed. In this case the Ring Buffer delivers faster the most recent image than the FIFO Buffer mode, which can be helpful for example if the image should be focused. Ring Buffer (in camera memory-standard mode) 1 2 As described the read out and recorded image is directly transferred into the internal buffer memory. Subsequently the next image is stored to the next free space. For the transmission from the camera to the computer always the most recent image is used, which works fine in case the computer does not do anything else than transfer images to its internal storage memory (RAM). 1 Now, if the transmission speed of the computer system is interrupted by e.g. software, it can happen that images get lost, because in Ring Buffer mode the most recent image has to be transmitted to the PC. In case of a delay in transmission, the image in the memory, which waits for transmission will lose its status as most recent image to the next image, and will be disregarded for transmission. Hence this image will be irretrievably lost. 2 Because Ring Buffer always transmits the most recent image and therefore the most real-time transmission, it is the ideal mode for e.g. adjustment of focus. FIFO Buffer (in camera memory) Like above the read out and the recorded image is directly transferred into the internal buffer memory. Subseqently the next image is stored to the next free space in memory. If all the memory space is occupied the camera stops storing. In FIFO buffer mode like name stated, FIFO = first-in first-out, the first stored image is read-out from the memory first and subsequently transmitted to the computer. This as well works fine in case the computer does not do anything else than transfer of images to its internal storage memory. Now, if the transmission speed of the computer system is interrupted by e.g. software, then still the first stored image is readout, and then the next image is read out and transmitted. However there could be a delay time (but need not). If now the slowdown causes the FIFO buffer to be completely filled, then the camera stops storing images and the next image will irretrievably lost as well, but in this mode it is lost before storing. This mode is ideal for users who need maximum transmission security; an image loss is very unlikely. 79 A5 BINNING WITH CMOS SENSORS Binning describes the summation of single pixels to form larger pixels and thereby improve the signal-to-noise ratio (SNR). Binning: CCD image sensors The term binning comes from scientific CCD image sensors. The prominent feature of charge-coupled-devices (CCD) is the lossless transport or shifting of charge packages until an amplifier circuit converts them into a voltage at the output, where the main readout noise contribution occurs. If charge packages from two or more pixels are added before they are read out (past the output amplifier) because of very low light signals; this process is called binning. Since the signal is increased before it is read out, and the image sensor’s readout characteristics remain unchanged, binning improves the SNR, and the image sensor’s resolution is reduced. The lossless transport feature of CCD image sensors makes binning possible. Binning: CMOS image sensors In general, binning is not possible in CMOS image sensors because voltages are processed and no charges are transported. In each pixel, the light generated charges are converted into voltages with the readout noise contribution of these amplifiers. Therefore, as opposed to CCD image sensors, if these voltages were combined, the readout noise would also be combined, which would not have the same positive effect on the SNR. Nevertheless, such a summation or even an averaging would be beneficial for the SNR, but with a smaller impact compared to CCD image sensors. Since such CMOS binning cannot be done within the image sensor, it either has to be done in the camera or in the computer. NOTE The current default binning mode is accumulation. Averaging is not available within Camware. CMOS binning 1 – accumulation: Pixel values can accumulate, causing an effective dynamic reduction or larger number formats, because the result might exceed the original format -- two times maximum 8 bit values will result in a 9 bit value. This will not be a problem if 12 bit values are accumulated and transported as 16 bit images. However, in the case of scientific CMOS, if 16 bit values are transmitted in 16 bit images, only two times 15 bit maximum values are allowed. The signal of the binned pixels will be accumulated. Due to the properties of readout noise; the increase of the noise itself will only be as big as the square root of the number of binned pixels. The SNR will improve and in addition, a reduction of the stored image data is achieved. CMOS binning 2 – averaging: Pixel values can be averaged, which has the same effect on the SNR as accumulation due to the properties of noise. This would keep the image output format the same and would reduce the amount of image data that can be stored. When this type of CMOS binning is processed in the camera it is called hardware binning. This should not be confused with real binning in CCD image sensors, because the hardware that processes this binning is not much different from the hardware in computer processing. Therefore, the term hardware binning may be misleading. 80 A6 LIGHTSHEET SCANNING MODE A6 LIGHTSHEET SCANNING MODE 4 Idle 1 3 The pco.edge Lightsheet scanning mode is a special mode for lightsheet microscopy and only available for Camera Link versions via SDK (not in Camware). It is based on Rolling Shutter mode and uses the readout mode single top down (Mode E see 6.9.3). 1 Standard line time (see p.6) value is 40 µs 22 and it can be set from camera specific line time (see p.6) up to 100 ms. An exposure area (between the orange bars, e.g. five lines 33 ) can be user defined. It is possible for the user to select the number of lines and the exposure time per line. This user-defined capability makes it possible to synchronize the pco.edge cameras with a lightsheet microscope that requires this method of camera exposure timing 2 Workflow (only PCO SDK) • Set Rolling Shutter mode • Set readout format E 1 • Set line timing on (0x0001 ≙ a CMOS_LINETIMING_PARAM_ON see sc2.defs.h) and set the appropriate line time 2 • Set number of exposure lines 3 • Set number of delay lines 4 • Trigger mode and acquire mode and hwio settings are free to select by the user SDK settings: (see SDK manual) SC2_SDK_FUNC int WINAPI PCO_SetCmosLineTiming wParameter 2 wTimeBase (e.g. µs 2 ) dwLineTime (e.g. 40 2 ) SC2_SDK_FUNC int WINAPI PCO_SetCmosLineExposureDelay dwExposureLines (e.g. 5 3 ) dwDelayLines (e.g. 4 4 ) 81 A7 IMAGE FILE FORMATS There are different file formats available for saving camera images with Camware. b16 The b16 16 bit format is similar to the bmp format. However, 16 bit pixel values are used instead of 8 bit pixel values. The file format consists either of a Basic Header (6 Long-parameter) or of an Extended Header (32 Long-parameter), the latter of which is optionally for additional information. There might follow a variable comment field (ASCII code). Finally, there is the actual data set that is saved linearly (as in the case of BMP files). With the exception of the first value, all parameters are Long Integers (4 Byte). The first 6 parameters must always exist. The rest of the parameters, as well as the comment field, are optional. 1 2 3 4 5 6 7 8 9 10 Parameter pcofile size header length image width image height extended header color mode b/w min b/w max b/w linlog Function the first 4 byte are the characters pcofile size in byte header size + comment filed in byte image width in pixel image height in pixel -1 (true), extended header follows 0 = black/with camera, 1 = color camera black/white LUT-setting, minimum value black/white LUT-setting, maximum value black/white LUT-setting, 0 = linear, 1 = logarithmic red LUT-setting, minimum value red LUT-setting, maximum value green LUT-setting, minimum value green LUT-setting, maximum value blue LUT-setting, minimum value blue LUT-setting, maximum value color LUT-setting, 0 = linear, 1 = logarithmic 11 red min 12 red max 13 green min 14 green max 15 blue min 16 blue max 17 color linlog 18 internal use 266 Comment file in ASCII characters with variable length of 0…XX. The length of the comment filed must be documented in the header length field. 16 bit pixel data line 1, pixel 1 value of the first pixel line 1, pixel 2 value of the second pixel … … We recommend that all images should be saved first in the b16 or TIFF format. The advantage is to have the b16 or tiff images available all the time. You will always have the maximum 16 bit information. Please note that not all image analysis programs can accommodate 16 bit data. The 8 bit format saves only the information displayed on the monitor screen. The 16 bit information will be lost and cannot be displayed later. 82 A7 IMAGE FILE FORMATS pcoraw This 16 bit pco file format is based on the new BigTIFF format, thus allowing for file size > 4GB. A new pco proprietary compression scheme is added in case it is necessary. Standard File Formats TIFF Tag Image File Format, version 6.0 and lower. There is a 16bit monochrome and color image format. BMP Windows Bitmap Format, b/w or color 8 bit format-images, which have been saved in BMP format can be loaded later only as 8 bit images, i.e. part of the original information (16 bit) is lost. FTS Flexible Image Transport System, Version 3.1. This is a 16 bit image format. The NASA/Science Office of Standards and Technology (NOST) has defined this format. Some programs use the FIT extension for this format. ASCII 16 bit format, some mathematical programs prefer ASCII data. JPG JPEG (named after the Joint Photographic Experts Group who created the standard) is a commonly used method of lossy compression for photographic images. The degree of compression can be adjusted, allowing a selectable tradeoff between storage size and image quality. JP2 JPEG 2000 is a wavelet-based image compression standard and coding system. It was created by the Joint Photographic Experts Group committee in the year 2000 with the intention of superseding their original discrete cosine transform-based JPEG standard (created 1992). AVI Audio Video Interleave is a multimedia container format introduced by Microsoft in November 1992 as part of its Video for Windows technology. MPG MPEG-1, similar to JPEG, is a standard for lossy compression of video and audio developed by the Moving Picture Experts Group (MPEG). WMV Windows Media Video (WMV) is a compressed video compression format for several proprietary codecs developed by Microsoft. The original video format, known as WMV, was originally designed for Internet streaming applications, as a competitor to RealVideo. 83 A8 CUSTOMER SERVICE A8.1 SERVICE The camera is designed to operate with no need of special adjustments or periodic inspections. A8.2 MAINTENANCE CAUTION NOTICE NOTICE NOTICE NOTICE UNPLUG CAMERA BEFORE CLEANING Risk of injury due to electric shock! Unplug the camera from any power supply before cleaning it. CLEANING Use a soft, dry cloth for cleaning the camera. Do not clean the input window unless it is absolutely necessary. Be careful and avoid scratches and damage to the input window surface. Do not use liquid cleaners or sprays. LENS CLEANING The lens is best cleaned with pressurized air or with liquid cleaners such as pure alcohol or with special optical cleaners that are available at high quality photo stores. Use a cotton swab dipped in pure alcohol or optical cleaning liquid and wipe only on the glass surface. Do not get any cleaning liquid on the metallic parts such as the lens thread, because tiny detached particles may scratch the surface. CLEANING LIQUIDS Aggressive cleaning liquids can damage your camera. Never use aggressive cleaning liquids such as gasoline, acetone, spirits or nitro cleanser. Every time the input window is cleaned, there is the possibility of surface damage. PROTECTIVE CAP Always store the camera with the protective cap or with a lens mounted to avoid dust and dirt on the input window. A8.3 RECYCLING If you want to dispose your camera, please send it to PCO or take it to a local recycling center. The camera includes electronic devices, which can contain materials harmful to the environment. These electronic devices must be recycled. 84 A8 CUSTOMER SERVICE A8.4 TROUBLE SHOOTING If you have a question, which is not adequately addressed in this manual, please contact PCO or your local dealer. To speed your request, we need the following information: Short description of the problem Description of your application Camera settings Type and version of camera Software being used Camera serial number Operating system (PC) Processor type (PC) Memory Graphic card Graphic card setup How to create a logfile: Enable log files: Repeat Open the Help Menu Save this file Or visit our website: ? Help Menu → Logging → Enable Logfile →Camware will ask you to press NO to activate Logfiles after restart of Camware the workflow which produces the faults Click Support Mail (+ Support File) → Camware will ask you: Generate support file? (CWSupport.zip – don’t rename it) and send it to PCO Support ([email protected]) http://www.pco.de/support/ and upload the support file with our support form Repair Before sending the camera for repair, first contact your local dealer or PCO respectively. When shipping the camera for repair, be certain to carefully pack the camera with proper shipping materials. If possible use the original packaging. Use the protection cap to protect the camera on the lens thread. Firmware, Software and Driver Update You will find all necessary software and drivers on the accompanying USB storage device. For the latest versions please check our website: http://www.pco.de/support/supportProducts/scmos-cameras/ 85 A9 INDEX NOTE: The mentioned page is always the starting page of a chapter! Key word Chapter Page Acquire mode Aquamatic 6.3.7 32 A3 64 Auto File Save 6.8.1 45 Averaging 6.5 38 6.3.5, A5 29, 80 A2 63 A4.1 68 6 13 Camera Link A4.2 69 Camera Link HS A4.3 73 Codec Dialog 6.9.2 48 Connection options 6.3.8 34 Contrast settings (Convert control) 6.3.9 36 Binning C-mount conversion Cable lengths Camware software Cuda nvidia driver 4.1 9 Demo mode (Camware) Dimensions 6.9.1 47 A1 58 Direct record to file 6.9.2 48 Double image mode 6.3.6 31 Driver installation 4.1 9 6.3.4 27 6.3.3 24 6.5 38 6.4 38 Infotext of recorded images 6.9.1 47 Input / Output possibilities 6.3.8 34 A4 68 6.9.7 53 Global Reset Global Shutter IIR Lowpass filter Image Overlay Interfaces Logfiles Lightsheet scanning mode Live preview Master Settings Rear connections Recorder modes A6 81 6.2; 6.9.4 14, 51 6.2 14 A1.6 62 6.3.7 32 6.5; 6.7 38; 42 Recorder memory settings 6.2 14 Region of interest (Sensor) 6.3.5 29 Rescan for cameras 6.9.3 50 6.3.7; A4.4.8 32; 79 20 Recorder Ring Buffer Rolling Shutter 6.3.2 Safety instructions Save / Open / Print files 2 7 6.9.2 48 Settings Overview 6.8 44 Sequence trigger mode 6.3.7 32 Short cuts 6.9.9 54 Support file 6.9.7 53 Time stamp Timing 6.3.7 32 6.3.1; 6.3.2; 6.3.3; 6.3.4 17; 20; 24; 27 Trigger Mode 6.3.1 17 USB A4.3 73 A3 64 6.9.9 54 Water cooling White Balance 86 ABOUT PCO ABOUT PCO pco. In 1987, PCO was founded with the objective to develop and to produce specialized, fast and sensitive video camera systems, mainly for scientific applications. Meanwhile the product range of PCO cameras covers digital camera systems with high dynamic range, high resolution, high-speed and low noise, which are sold in the scientific and industrial market all over the world. Currently PCO is one of the leading manufacturers of scientific cameras. Worldwide representatives, together with our own sales department and technical support assure that we keep in touch with our customers and their needs. The actual wide range of specialized camera systems is the result of technical challenge and product specific know-how. A design according to advanced techniques, a high standard of production and strict quality controls guarantee a reliable operation of the cameras. Our own developments in conjunction with an excellent contact to leading manufacturers of image sensors ensure our access to state-of-the-art CCD- and CMOS-technology for our cameras. Since 2001, PCO is located in its own facility building in Kelheim at the shore of the beautiful and international river Danube. Here in the county Bavaria, which is well known for its excellent support and conditions for high technology companies, we share the benefits of the simple access to high performance products and services in the surrounding area. Kelheim itself is a historical town, first documented in 866. The small city is founded at the confluence of the Danube and the Altmühl, which has been converted into the Rhine-Main-Danube bypass channel for water transport. Located in Danube-valley, it is the heart of a beautiful river and forest covered lime plateau landscape. It´s landmark, the Hall of Liberation, was built by Ludwig I. in 1863 on the Mount Michael and is visible from all over the city and valley. The beautiful Danube-Gorge, which is protected as natural monument since 1840, is located between Kelheim and the famous abbey Weltenburg. 87 pco.
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