CL3 User Manual

CL3 User Manual
Operation Manual
IEC®Centra CL3 Series
Centrifuges
VENTILATED
Cat. No.3750: 120 VAC, 60Hz
Cat. No.3751: 220, 230, 240 VAC, 50/60 Hz
REFRIGERATED
Cat. No.3755: 120 VAC, 60Hz
Cat. No.3756: 220, 230, 240 VAC, 50 Hz
Cat. No.3757: 230 VAC, 60 Hz
OM3750 Rev. 5
11 January 2004
Analyze Detect Measure Control ™
Contents
CONTENTS
1. Introduction....................................................... 3
1.1 Product Description.......................................................4
1.2 About This Manual ........................................................4
2. Installation ........................................................ 4
2.1 Receive the Unit............................................................4
2.2 Prepare the installation site ...........................................4
2.3 Verify Power Configuration............................................5
2.5 Moving the Unit .............................................................5
3. Operation.......................................................... 5
3.1 Rotor and Accessories ..................................................5
3.2 Starting and Stopping a Run .........................................6
3.3 Timing Mode..................................................................7
3.4 Stored Programs ...........................................................7
3.5 Refrigeration (refrigerated systems only) ......................8
3.6 Front Panel....................................................................9
3.7 Diagnostic Messages and Error Codes.......................10
4. Applications .....................................................11
4.1 Introduction ................................................................. 11
4.2 Speed and Force Tables .............................................12
4.3 Derating Tables for Dense Samples............................17
4.4 Chemical Resistance Table.........................................18
4.5 Decontamination Table................................................19
4.6 RCF Nomograph .........................................................20
5. Maintenance................................................... 21
6. Specifications ................................................. 22
OM31150 Rev. 5 11 January 2004
Page 3 of 24
1. Introduction
2. Installation
1.1 Product Description
2.1 Receive the Unit
The IEC Centra CL3 Series units are high-speed, multipurpose centrifuges used in medical, industrial, and
scientific applications. They are available in two models:
All units are shipped in protective packaging.
• Ventilated (CL3)
• Refrigerated (CL3R)
Both units accommodate a wide variety of rotors including
fixed angle, swinging bucket, and fixed horizontal designs.
They can process tubes, bottles, microplates,
microcapillary tubes and microsample tubes. The units can
centrifuge up to one liter of fluid in a single operation.
Operation Modes
There are two operation modes:
• Manual Mode: to enter temperature (refrigerated units
only), speed/force, and time values for individual runs
• Program Mode: to define and save a maximum of 99
specific sets of run parameters, to recall and reuse
Timing Modes
Three timing modes are available on each unit:
• Automatic timed run
• Short spin (momentary)
• Hold (continuous mode).
Other Features
Acceleration and brake rates may be controlled to optimize
runs; rapid for fast separations or slow for delicate
samples. Repeat runs, with the same speed and time
settings, may be achieved at the touch of a key.
A fail-safe cover interlock insures that the cover is closed
before a run can begin, and keeps the cover closed until the
rotor has reached a safe low speed (below 100 rpm), even
in the event of a power failure.
The rugged steel cabinet and rigid construction provide
quiet operation and long-term reliability.
1.2
About This Manual
Contents
This manual contains information to install, operate, and
maintain IEC Centra CL3 Series Centrifuges. Differences
between the refrigerated and ventilated models are noted in
the text. This manual also contains speed and force,
derating, chemical resistance, and decontamination tables.
Audience/Purpose
This manual is written for centrifuge operators. In addition
to operation information, it contains a few basic
troubleshooting techniques, and a chapter on maintenance.
This manual is not a guide for servicing centrifuge units.
Page 4 of 24
1. Follow the unpacking instructions on the carton.
2. Inspect the unit upon receipt and immediately file any
damage claims with the shipper/carrier.
3. Complete and return the postage-paid warranty card.
2.2 Prepare the installation site
The unit normally resides on a bench top.
1. OPTIONAL:
Ventilated models can be placed in a cold room (no
colder than 4°C), for processing temperature-sensitive
samples.
! CAUTION
When you remove the centrifuge from a cold
environment, do not operate for a minimum of two
hours to allow condensation to evaporate prior to
use.
NOTE: When used in a cold room environment, some
bearing noise may become evident. The bearing
lubricant thickens at low temperatures. As the
centrifuge speeds up, it is thinned and distributed more
evenly. Once this occurs, any noise should subside.
2. Place the centrifuge on a clean, dry surface, to make
certain that the suction feet at the bottom grip the
surface firmly. Keep the area beneath the unit free of
debris and loose materials.
! CAUTION
The resting surface must be level, to ensure quiet,
vibration-free operation. A rigid and stable location is
important. An improperly loaded centrifuge may
vibrate or move
3. Locate the centrifuge to allow a clearance of 8 cm
(3 inches) on all sides of the unit, to ensure proper
ventilation.
Dimensions:
IEC Centra CL3R
Sample Loading Height
Cover Closed Height
Cover Open Height
Width
Height
IEC Centra CL3
12.5” (32 cm)
14.5” (37 cm)
33” (84 cm)
26.5” (68 cm)
19.25” (49 cm)
21” (53 cm)
! WARNING
International Electrotechnical Commission standard
1010 part 2-20 limits the permitted movement of a
laboratory centrifuge to 12” (300 mm) in the unlikely
event of a disruption. Laboratory management
procedures should require that no person or any
hazardous materials enter within this boundary while
the centrifuge operates.
OM3750 Rev. 5 11 January 2004
2.3 Verify Power Configuration
1. Verify that the correct power cord and connector is
provided for your installation.
The unit requires a grounded power supply (3-outlet).
If your facility does not have grounded power outlets,
arrange for proper grounding. The power cord plugs in
on the left side of the unit.
!
WARNING
ELECTRICAL HAZARD!
Do not remove the grounding pin from the
centrifuge power cord. Do not use the bare
wired power cord to attach a power plug that
does not have a grounding pin.
The power cord provided with the unit is
correctly rated for the highest current demand.
This power cord should not be interchanged
with cords from equipment with lower current
demand. Exchange of power cords between
equipment may create a fire hazard.
2. For best results, the refrigerated centrifuge should be
used on a dedicated line. Variations in line voltage or
frequency affect the unit’s speed and other
characteristics.
NOTE:
Less than nominal line voltage may prevent the
centrifuge from reaching maximum published
specifications of speed and/or temperature. Power line
voltage, at some locations, may sag when the
refrigeration system turns on
2.4 Circuit Breaker
The system provides an automatic circuit breaker for
emergency situations such as power surges that could
damage the unit. If the circuit breaker trips:
1. Unplug the unit.
2. Press the white button, on the left side of the unit.
3. Plug the unit back in.
2.5 Moving the Unit
Suction cups at the bottom of the unit keep it anchored to
the work surface. Keeping the unit stationary is a safety
feature.
To move the unit to a new location:
!
WARNING
The unit can weigh up to 145 lb. (65.8 kg).
Use caution when moving to avoid any injury.
1. Check that the new site meets the criteria in Section
2.2 before moving the unit.
2. Position a flat object, such as a tongue depressor, near
a suction cup at the bottom of the unit.
3. Lift up an edge of the cup, and insert the flat object far
enough to break the vacuum suction seal.
4. When all four suction cups are disengaged, lift the unit
from the work surface.
5. When the unit is in its new location, ensure that the
suction cups adhere correctly to the work surface
OM31150 Rev. 5 11 January 2004
3. Operation
3.1 Rotor and Accessories
A balanced load is essential for all centrifuges. An
unbalanced load produces vibration, and can damage the
unit. ALWAYS balance containers on opposite sides of the
rotor.
NOTE: A 2-gram load imbalance, at a speed of 4600
RPM, imparts force equivalent to 20 pounds (9.1 kg) at
rest. Always ensure that the rotor is loaded
symmetrically, with a full complement of accessories,
and a full (or paired) set of tubes. Tube adapters should
also be installed symmetrically.
Balance load within 1 gram
The rotors are dynamically balanced at the factory. The
manufacturer matches removable parts (trunnion rings,
shields, buckets, and carriers) to within 1 gram, and
stamps the weight on each piece. Check these markings,
whenever you interchange parts, to ensure that opposite
parts are matched. Ensure that the total weight of samples
and removable parts, loaded in opposing positions, are
equal in weight, to within 1 gram. The position numbers,
present on many rotors and adapters, identify opposing
tube positions.
Opposing containers must be alike in shape, thickness, and
distribution of glass or plastic. This is especially important
for large containers.
NOTE:
Swinging Bucket Rotors:
Tubes loaded into swinging bucket rotors must be
symmetric, around the axis of rotation. Verify this by
rotating the entire rotor 180° by hand.The loads should
be in the same apparent positions (not mirror images).
In addition, the loads within each bucket must, also, be
symmetric around the bucket’s pivot axis. Verify this
by ensuring that each bucket is loaded so that it does
not tilt from the vertical, when the rotor is at rest.
Maintaining balance within each bucket ensures that the
bucket and the tubes swing out to horizontal, when the
rotor reaches operating speed, applying centrifugal
force toward the bottom of the tubes. Failure to achieve
full swing-out causes vibration and premature wear of
the rotor and the motor.
Samples of like (similar) specific gravities may be
processed in the same run, provided that the samples of
the same type are balanced around the rotor, as though
they were the only pairs in the rotor.
! CAUTION
Do not exceed maximum rated speed for each rotor/
accessory combination. Maximum rated speeds can be
found in Section 4.2 - Speed And Force Tables.
Rotor Balance
Load tubes in the following manner:
Page 5 of 24
1. Load two tubes at
positions: 9 and
18.
2. Load four tubes at
positions:
1, 5 and 10, 14
or
7, 3 and 16, 12
3. Load six tubes at
positions:
1, 9, 5 and 14, 18,
10
or
7, 9, 3 and 12, 18,
16
4. Loading an odd
number of tubes is
not recommended.
Avoiding Vibration
All centrifuges have critical speeds at which vibration
occurs. As the speed increases beyond the critical speed,
vibration will cease. This inherent condition also occurs
during deceleration. An imbalanced load intensifies these
critical vibrations. You should avoid running the centrifuge
at or near its “critical speed” to avoid excessive vibration.
Rotor Installation
To install the rotor:
1. Press the disk (located on the underside of the rotor) to
the rotor.
2. Slide the rotor over the shaft, until it snaps into place.
Release the disk.
3. Pull the rotor straight up, to insure a positive lock.
Rotor Adapter
To install the shaft adapter for an existing rotor:
1. Press the adapter disk and slide the adapter over the
rotor shaft. Release the disk.
2. Pull the adapter straight up to insure a positive lock.
3. Align the rotor keyway with the key on the shaft
adapter and place the rotor onto the adapter.
4. Tighten the rotor locking nut (with the provided
wrench), until it tightly engages the rotor.
To remove existing rotors from the shaft adapter:
1. Remove any sample tubes, shields, buckets, and other
accessories, from the rotor.
2. Unscrew and remove the locking screw.
3. Grasp the rotor and rock it front-to-back, and side-toside.
4. Remove the rotor from the shaft.
3.2 Starting and Stopping a Run
The display above the Program key shows the unit’s
operating mode:
• blank
• 1-99
The unit is in manual operation.
The unit is under control of the displayed
stored program number.
•C
The unit is set to Rapid Condition, a special
program discussed in Section 3.4.
The rest of the display indicates the last parameters
selected.
Manual Operation
For manual operation:
1. Press the MANUAL key so that the program display is
blank.
2. Select the desired temperature (refrigerated system
only).
3. Select the speed/g-force, run time, acceleration mode,
and braking mode.
4. Press START to start the spin.
The time display counts down, and displays the time
remaining in the current spin, during manual operation.
The specified run time begins when the START key is
pressed. Braking begins when the set time elapses. Run
time does not include braking time.
The spin will stop automatically at the end of the desired
interval.
Rotor Removal
To remove the 243 rotor:
1. Press the disk (located on the underside of the rotor) to
the rotor.
2. Lift the rotor straight up.
3. Release the disk.
Page 6 of 24
IMPORTANT:
A run can be stopped at any time by pressing the STOP
key.
The settings can be changed during a manual run. These
changes affect the run in progress. If the time setting is
changed during a run the unit adjusts the count-down timer
to display the revised setting as the total time of the run. If
the new time selected is less than the elapsed time, the run
will end.
The unit’s mode (settings) cannot be changed during a
program mode spin.
OM3750 Rev. 5 11 January 2004
3.3 Timing Mode
Four timing modes are available on these units: To select a
timing mode, press the down arrow under the time display
until the time goes below zero, and the appropriate symbol
is displayed.
Momentary Mode (---) - Momentary spin is useful for easily
separated samples, for simultaneous mixing of samples,
and to deposit condensate droplets at the bottom of the
tube.
For momentary spin, set the mode so that three dashes
(---) appear above the Clock icon. Select temperature
(refrigerated models only), speed/g-force, acceleration
mode, and braking mode, as for manual operation.
Press and hold the START key. The run starts when you
press the key, and ends when you release the key. In this
mode, you can perform very quick separations or protocols.
During a momentary spin, the unit displays actual values,
not desired settings. The time display counts upward, and
displays the elapsed time since the START key was
pressed.
Hold Mode (HLd) - For hold mode (operation without preset
time limit), set the mode so that HLd appears in the Time
display.
Select temperature (IEC Centra CL3R only), speed/g-force,
acceleration mode, and braking mode, as with manual
operation. Press and release the START key. The run
starts when you press the key, and stops only when you
press the STOP key. Hold mode is like manual operation,
except that the time setting is not used. During a run in
hold mode, the time display counts upward, and displays
the elapsed time of the spin.
At-Start Mode (ACC) - The set time will start counting
down at the beginning of acceleration (when the start key
is pressed). The unit is originally set to this mode.
At-Speed Mode (SPd) - The set time will start counting
down, when the rotor has reached 95% of set speed. The
display will alternately show the set time and SPd, during
acceleration to 95% of the set speed.
To select a timing mode, press the Time down arrow key,
and scroll below 0 minutes. ACC, SPd, HLd, or --- will
appear. Press and release the Time down arrow key, to
choose the desired mode. After three seconds, or after
pressing the PROGRAM or START keys, the display will
return to the last selected run time, provided the up arrow
key has not been pressed.
The timing mode is stored along with the other parameters,
when a program is saved. If a saved program has been
recalled, altered, and not re-saved, the timing mode will
revert back to the previous mode, when recalled.
3.4 Stored Programs
The centrifuge has an internal memory capable of holding
99 sets of run parameters. Programs are retained in
memory even if the power is turned off. When necessary,
a program can be modified for a particular run or changed
permanently.
NOTE: You cannot change the unit's program, rotor/
radius, or timing modes during a spin.
OM31150 Rev. 5 11 January 2004
Add/Change Program
1. Press PROGRAM to enter Program mode.
2. Select a program number with the numeric keypad. The
current program parameters will appear on the display.
3. Modify the desired parameters including speed/RCF,
time and temperature (if a refrigerated unit). Use the
numeric keypad or modify the ACCEL or BRAKE modes.
Additionally, a radius value may be stored explicitly in
the program.
NOTE: The program number will flash indicating that the
program was changed but not saved.
4. To make changes temporary, press START.
To make changes permanent, press the SAVE key.
NOTE:
After pressing the SAVE key, the program number will
stop flashing. The program will remain in memory until
further changes are made.
Because the centrifuge has a fully automatic rotor
recognition system, the very first rotor spun using a
particular program will be identified and have its identity
automatically stored in the program. If you did not set a
radius value, the default radius (or maximum allowable
radius) for that rotor will be stored with the program as
well. If you attempt to run a rotor other than the one
whose identity is stored in the program, a “ch hd”
(check head) message will appear.
To clear the rotor and its radius from memory, press the
SAVE button. The very first rotor spun after SAVE is
pressed will have its identity stored in the program,
together with its default radius (if not explicitly entered
to the program).
Recall Program
1. Press the PROGRAM key to enter program mode.
2. Select the appropriate program number by entering the
desired program number on the numeric key pad and
press ENTER. The program’s set parameters will
display.
3. Press START, to begin this run.
Lock Program/Unlock Program
• To lock a program to prevent changes to the program’s
parameters:
1. Select the desired program on the numeric
keypad.
2. Press the SAVE key three times.
When you scroll to a locked program, the letter L will
flash in the program display after the program number is
displayed.
• To unlock a program to change parameters:
1. Select the desired locked program on the numeric
keypad.
2. Press the SAVE key three times.
Page 7 of 24
3.5 Refrigeration (refrigerated systems only)
Refrigerated units refrigerate the rotor chamber to a
specific temperature whenever the cover is closed and the
unit is switched on.
NOTE: If you use the keypad and momentarily display a
cold temperature (stepping through stored programs, for
example), refrigeration will not be activated.
! CAUTION
If the rotor chamber is not at the specified
temperature, it will NOT abort a spin. Press the
STOP key if you need to stop a run.
At the beginning of a spin, if the chamber
temperature differs by more than 5° C from the
specified temperature, the temperature display will
switch between the actual and set/programmed
temperatures until the two temperatures come to
within 5° C.
Rapid Condition
When the chamber temperature (refrigerated models only)
is above the set temperature, Rapid Condition will run the
243 rotor at 4000 rpm and all others at 2800 rpm to
increase air circulation in the chamber, to quickly cool the
chamber to the set point. When the chamber temperature
is below the set temperature, Rapid Condition will run the
rotor at 2800 rpm, to warm the chamber to the set
temperature. When the temperature has been reached,
three beeps will sound and the rotor will brake to rest.
Some smaller rotors may not be able to warm the chamber
to higher temperature settings.
To select this program, press the Program arrow keys, until
a C appears in the Program display. Select the desired
temperature, install a rotor, and press the START key.
Page 8 of 24
OM3750 Rev. 5 11 January 2004
3.6 Front Panel
Speed/Force display:
The rotor speed in RPM or
force in RCF. Press this
symbol to toggle between
RPM and RCF. Use the
arrow keys to change the
set speed or force. The
display shows speed within
50 RPM. It never requires
calibration. Select speed in
increments of 100 RPM,
from 1,000 through 8,500
RPM. Select RCF in
increments from 1 - 1,000
xg by 50 xg and above
1,000 xg by 100 xg. The
numeric display can also
display warning or error
messages.
Time display: The number in the display
(above this symbol) indicates time. Below
ten minutes, time is displayed as
minutes:seconds. Above 10 minutes, just
minutes are displayed.
Time is set in:
1 second increments from
1 - 59 seconds.
15 second increments from
1 - 5 minutes.
1 minute increments from
5 - 99 minutes.
In normal timed mode, the system counts
down from the set point. In time Hold or
momentary spin modes, the system counts
up.
In At-Start mode, the timer begins counting
at the start of a run. In At-Speed mode, the
timer begins counting when the rotor
reaches 95% of the set speed.
Rotor/Radius Key: The display (above this symbol) indicates the
selected rotor number or the rotor radius, in centimeters. The
rotor/radius key toggles between the two displays. The
applicable Thermo Electron rotor numbers are supplied in the
memory, along with their maximum radii, in centimeters. To
select a rotor number, toggle to ROTOR, and press an arrow
key under the rotor/radius display. To change the radius,
toggle to RADIUS, and press an arrow key under the rotor/
radius display. Note that the radius cannot be changed to a
radius larger than the maximum radius, or less than the
minimum radius, for the selected rotor.
Program key: This key saves
the currently displayed
desired settings as stored
programs 1 through 99. The
numeric display (above this
symbol) shows the stored
program number and mode
of operation.
Temperature display:
The number in the
display (above this
symbol) represents
temperature in degrees
Celsius, from − 9 °C
through + 40 °C
(Refrigerated only).
This key starts a run. A
run is governed by the
Set parameters (manual
or programmed). The
associated green light
blinks, until the rotor
reaches 95% of the set
run speed. The light
stays on until the end of
the run.
RPM
Rotor
RCF
Radius
This key controls rotor acceleration up
to 400 RPM. If the yellow light over the
rabbit is lit, full acceleration is selected.
If the yellow light over the turtle is lit,
slow acceleration is selected. Slow
acceleration takes from 15 to 35
seconds to achieve 400 RPM,
depending on the rotor and its contents.
After 400 RPM, full acceleration is
applied until the set speed is reached.
This key controls rotor braking. If
the yellow light over the rabbit is lit,
full braking is selected. If the
yellow light over the turtle is lit,
slow braking is selected. (This
means the rotor will coast down
from 500 RPM.) If both lights are
out, all braking is disabled and the
rotor will coast from operating
speed to a stop.
This key unlocks
the cover. This key
is inoperative if a
run is in progress.
Pressing it will not
stop the run. The
cover will not
unlock until the
rotor has slowed to
below 100 rpm.
Use the arrow keys to view or change the Set parameters for Speed/Force, Time, Temperature (Refrigerated only) Rotor/
Radius, or Program. The first time the key is pressed the numeric display switches from Actual readings to Set parameters,
without changing them. If you press the key a second time the selected parameter increases or decreases once for each
depression. If you hold the key down, the setting will keep changing until you release the key.
The longer you hold the key the more rapidly the setting changes. Hold a key down to approach a desired setting. Then
press the up or down key repeatedly to select the exact setting. When you release the arrow keys for 3 seconds the display
returns to the Actual readings.
OM3750 Rev. 5 11 January 2004
This key stops a run.
(A run will also stop
automatically when
the set time has
elapsed or the start
key is released in
momentary mode.)
The red light will
flash to indicate the
rotor is still slowing
down (braking or
coasting). When the
run ends the red light
stays on indicating
that the rotor has
stopped.
Page 9 of 24
3.7 Diagnostic Messages and Error Codes
On/Off key: The On/Off key must be activated
to enable use of the front panel. This key
applies power to the control panel and
refrigeration system (Refrigerated model only).
The On/Off key is inoperative during actual
runs. Stop a run with the STOP key.
Refrigerated models display chamber
temperature whenever they are plugged in, but
will not cool down if the unit is off. The red
STOP light indicates that the centrifuge is
plugged in.
The control panel contains numeric displays for
RPM/RCF (Speed/Force), Time, and
Temperature (Refrigerated only). These
displays have two states or modes: Actual
(bright display) and Set (dim display).
In Actual mode (bright display), they indicate
current run conditions, such as rotor speed or
force elapsed time of, or time remaining in, the
run actual temperature (Refrigerated only).
The beeper sounds in these situations:
• Two times on power up.
• Three times at the end of a spin.
• Five times when a warning occurs
Diagnostics
Diagnostic messages appear in place of the speed display
in the following cases:
Message
bAL
LId
PFAIL
In Set mode (dim display), the display indicates
the desired settings for the run.
Drive Fault The drive fault error can occur due to one of
the following conditions:
- the motor experiences an abnormally high current or
voltage condition
The numeric displays can also, display warning
or error messages
Acceleration and
Braking
Gentle acceleration and braking can be
selected when centrifuging delicate samples.
The gentle settings avoid mixing of density
gradients or breakup of pellets.
Description
bAL indicates an unbalanced rotor.
Open the cover to erase this message.
Verify that a balanced load is installed. Inspect the rotor and
rearrange the tubes, or add additional tubes with fluid to
balance the rotor.
LId appears if you press the START key when the cover is
not closed. Close the cover to erase this message.
PFAIL indicates that power was interrupted during a run.
This message appears when the unit is turned back on
following the failure. The front panel will alternate between
the PFAIL message and the remaining run time (or elapsed
time if in Hold mode). Press STOP to erase this message.
Press START to resume the previous run.
dFL
- the drive PCB operating temperatures exceed 700C
- a brake circuit fault has been detected
- a short to ground has occurred in the motor
The rotor will coast to a stop. To clear the error, unplug the
unit (or turn power switch off) and reconnect the unit (or
turn power switch on). Check for obstruction of the cooling
fans or vents. If repeated dFL errors occur, contact service
personnel.
Page 10 of 24
OM3750 Rev. 5 11 January 2004
Error Codes
An error code typically means that the internal
microprocessor has detected impermissible readings or a
failure in the unit. Error messages appear in the speed
display. When an error code is displayed, unplug and
reconnect the unit to power. If the error code reappears,
factory-authorized maintenance may be required. Contact
your local Thermo representative and tell the service
personnel which message appeared when you report the
problem.
Error Code
Description
No Tachometer
Tachometer signals were not present during the run. The
Err 1
rotor coasts to a stop. Cover opening is inhibited after this
error. Unplug and reconnect the unit to power to reset this
error.
Overspeed
OSPd
Speed is 200 RPM above the maximum speed for the
installed rotor. The rotor will brake to a stop.
Refrigeration Failure (Refrigerated only)
rEFR
The unit displays this code if the measured temperature
exceeds 45°C, at any time during the run.
Fail-safe Time out
Independent circuitry on the circuit board has sensed a lack
FSAFE
of activity from the control microprocessor. All power
circuits (including motor, latch, solenoid, etc.) are disabled.
Cop Watchdog/OpCode Trap Error
The microprocessor has sensed a lack of activity from the
COPF
program that controls the centrifuge. The rotor will coast to
a stop.
COP Watchdog Not Enabled
COP
The microprocessor COP is not enabled. The rotor will
coast to a stop.
Undefined Interrupt
UndFl
The microprocessor was interrupted by an undefined
source. The rotor will coast to a stop.
Op-Code Trap Error
ILLOP
The rotor will coast to a stop
Wrong Direction of Rotation
dIR
The microprocessor discovered wrong direction of rotation,
during acceleration
Warnings during a spin: LId, PFAIL, and dIR error messages can occur
during a spin. In this case, the rotor brakes or coasts to a stop and the run
ends.
4. Applications
4.1 Introduction
This section describes the use of specific rotors and
accessories. More detailed information is shipped with the
rotor or accessory itself. This section contains five
reference sections:
• Speed and Force Tables
• Derating Table for Dense Samples
• Chemical Resistance Table
• Decontamination Table
• Nomograph
! CAUTION
Do not exceed maximum rated speed for each rotor/
accessory combination. Maximum rated speeds can be
found in Section 4.2 - Speed And Force Tables.
Relative Centrifugal Force (RCF or G-force) at a given speed
varies with the rotor, and with the distance away (rotating
radius) from the shaft of the centrifuge (center of rotation).
The rotating radius is measured to the furthest inside tip of
the tube, away from the centrifuge shaft. The Speed and
Force Tables indicate the maximum speed and RCF that the
IEC Centra CL3(R) can achieve, with various rotor/
accessory combinations. The Derating Table specifies
reductions in maximum RPM, when spinning samples with
specific gravity above 1.2.
Use of any tube above its rated RCF can cause tube
cracking. To avoid this, compare the G forces, specified in
the Speed and Force Tables, with the ratings for the tubes
that you are using. If the tubes are not rated for the force
that the centrifuge will apply, look up their reduced g force
rating and enter it on the control panel.
Corrosive Solvents
Your centrifuge is made of materials designed to resist
immediate attack from most laboratory chemicals.
Prolonged exposure should be avoided, by immediately
removing the chemical from rotor or assembly. Rotors and
accessories placed in the chamber are made of a variety of
materials, including aluminum and polypropylene. The
Chemical Resistance Table shows the suitability of each
material with different classes of reagents.
Section 5.2 describes how to clean and remove corrosion
from the chamber, rotors, and accessories. Follow these
instructions and clean spills promptly to minimize the effect
of corrosive chemicals and to avoid expensive repairs.
OM31150 Rev. 5 11 January 2004
Page 11 of 24
4.2 Speed and Force Tables
Rotor 243 4-Place Swinging Bucket
Complete with 4 buckets, but without adapters.
Tube
Maximum
RPM / RCF
No. x Vol. (ml)
Description
4 x 250 ml sealed
4 x 250 ml unsealed
4 x 175/225 mL sealed
4 x 100 ml sealed
4 x 80 ml
Thermo Electron 2502
Corning 1260-250, 1280-250 †
Falcon 2076/2075 conical
Round bottom, 38x111 mm
Amicon Centricon Plus 80
Corning 8240-50 †,
Thermo
Electron 2997
Falcon/Corning conical
Universal 25x107mm †
Amicon Centricon Plus 20
Falcon/Corning conical
Kova/Urisystem †
16.5x103mm †
Vacutainer 16x100, 16x125mm
Falcon 2059 round with cap ‡
Vacutainer 16x75mm †
Hemogard Vacutainer
13x75mm, 13x100mm
10x75, 12x 75mm RIA tubes,
Falcon # 2063
Microtubes
Microtainers
PCR microtubes
microtubes
microtubes
8 x 50 mL sealed
8x50ml sealed
12x25ml sealed
4 x20 ml
20x15ml sealed
20x10ml sealed
32x10ml sealed
36x10-15ml sealed
20x14ml
36x7ml sealed
48x5-7ml sealed
48x3-5ml sealed
48x1.5/2ml sealed
48x0.7ml sealed
48x0.5ml sealed
48x0.4ml sealed
48x0.25ml sealed
AdapterRadius
Aerocarrier™ Adapter
Quantity x Cat No*
Other
4000
4000
4000
4000
4000
2970
2970
3150
3110
2970
16.6
16.6
17.6
17.4
16.6
2pr
2pr
2pr
2pr
2pr
x6558E
x6558E
x6559E
x6557E
x6558E
-
4000
3150
17.6
2pr x6556E
-
4000
4000
4000
4000
4000
4000
4000
4000
4000
3130
2680
3150
3150
3150
2860
3150
2700
2700
17.5
15.0
17.6
17.6
17.6
16.0
17.6
15.1
15.1
2pr
2pr
2pr
2pr
2pr
2pr
2pr
2pr
2pr
x6560E
x6568E
x6556E
x6561E
x6561E
x6567E
x6562E
x6563E
x6563E
-
4000
3150
17.6
2pr x6566E
-
4000
3150
17.6
2pr x6566E
-
4000
4000
4000
4000
4000
2110
2110
2110
2110
2110
11.8
11.8
11.8
11.8
11.8
2pr
2pr
2pr
2pr
2pr
x6565E
x6565E
x6565E
x6565E
x6565E
2pkx5763
2pkx5763
2pkx5764
2pkx5764
* Adapters with Number Series 65XX are sold by the pair (pr). Adapters 5763, 5764 are sold by the pack/12.
Note: Aerocarrier Adapters comply with the aerosol containment requirements of:
OSHA 29 CFR Part 1910.1030 Occupational Exposure to Bloodborne Pathogens; Final Rule dated 12/06/91.
† Check with tube/bottle manufacturer for current maximum rating, as Thermo Electron Rotor/Accessory combination may exceed
that rating.
‡ Caps have larger diameter than tube and interfere with each should all cavities try to be used. Thus, use only center hole and 12, 3, 6
and 9 o'clock positions in the adapter for a total of 5 tubes per adapter.
Aerocarrier is a trademark of Thermo Electron Corporation.
Corning is a registered trademark of Corning, Inc.
Amicon and Centricon are trademarks of Millipore Corporation.
Vacutainer and Microtainer are registered trademarks of Becton Dickinson Company.
PCR is a registered trademark of Perkin Elmer Corporation.
Rotor Packages 52151, 52152, 52153, 52154
Rotor 215 4 Place Horizontal Swinging Bucket
(Use requires 50968 tapered shaft adapter.)
No. of
Places
Tube
Volume
(ml)
Trunnion/
Shield or
Carrier
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
4
50
325/320**
4100
2650
14.0
30 x 116
4
4
8
12
12
16
20
50
10
50
10
10
5-7
3-5
350/323**
310/356
326/320
355/356
366/1013
366/1018
366/369
3800
4300
3600
3600
4100
4150
4600
2050
2750
2050
1900
2400
2450
2625
15.2
13.2
14.2
13.2
12.8
12.8
11.1
Falcon/Corning Conical
17.2 x 113
30 x 116
17.2 x 112
16.5 x 107 16 x 75; 16 x 100
14.2 x 103 13 x 75; 13 x 100
12.6 x 83 10-12 x 75
4
3
1024
2050
500
10.6
1” x 3” Microscope Slide
O.D. x Length (mm)
Common Tube Size
* Order adapter 1106 and 571 cushion, to spin 15 ml Falcon/Corning Conical Tubes.
OM3750 Rev. 5 11 January 2004
Page 12 of 24
Rotor Package 52212
Rotor 221 6 Place Horizontal Swinging Bucket
(Use requires 50968 tapered shaft adapter)
No. of
Places
6
6
Tube
Volume
(ml)
Shield
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
O.D. x Length (mm)
10
356
4400
2800
13.0
17.2 x 102
15
303
4000
2600
14.6
17.2 x 119
Falcon Corning Conical
Kimble 45168-125
Rotor 244 2-Place (Tray) Horizontal Microplate
(Use requires 50968 tapered shaft adapter)
No. of
Places
4
2
Tube
Volume
(ml)
Carrier
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
Width x Length (mm)
-
included #
4000*
1800
10.0
86 x 128 (96 well) Microplates
-
49852
4000*
1800
10.0
86 x 128 x 55mm Deep Plates
* Replacement carrier is Thermo Electron No. 49852
** See derating table for 244 rotor
Rotor Package 58012
Rotor 801 6-Place Fixed Angle 45°
(Use requires 50968 tapered shaft adapter)
No. of
Places
Tube
Volume
(ml)
6
50
50
6
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
305
4600
2950
12.1
320
5200
3300
10.8
Shield
O.D. x Length (mm)
30 x 133
Falcon Corning Conical*
30 x 116
Corning 8300-50
*With Thermo Electron 315 Conical Cushion
Rotor 804S 4-Place Fixed Angle 45° with 323 Sealed Dome Shields
(Use requires 50968 tapered shaft adapter)
No. of
Places
4
4
Tube
Volume
(ml)
Shield
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
O.D. x Length (mm)
50
323*
7200
6675
11.5
Falcon/Corning Conical
60
341
2500
1000
14.3
Corning
* Order adapter 1106 and 571 cushion, to spin 15 ml Falcon/Corning tubes.
Rotor Package 58092
Rotor 809 12-Place Fixed Angle 45°
(Use requires 50968 tapered shaft adapter)
No. of
Places
12
12
12
Tube
Volume
(ml)
Shield
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
O.D. x Length (mm)
10
356
5300
3200
10.2
17.2 x 88
15
303
4800
2880
11.2
17.2 x 102
15
302
4200
2480
12.6
17.2 x 122
Falcon/Corning Conical
OM3750 Rev. 5 11 January 2004
Page 13 of 24
Rotor Package 58153
Rotor 815 24 Place Fixed Angle 33°
(Use requires 50968 tapered shaft adapter)
No. of
Places
Tube
Volume
(ml)
Shield
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
O.D. x Length (mm)
24
24
24
10
356
4800
2860/2340
11.1/9.1
17.2 x 88
15
303
4200
2360/1970
12/10
15
302
3800
2100/1800
13/11
17.2 x 102
17.2 x 122
Falcon Corning Conical
Rotor Package 58162
Rotor 816 8-Place Fixed Angle 33°
(Use requires 50968 tapered shaft adapter)
No. of
Places
Tube
Volume
(ml)
Shield
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
8
50
320
4600
2800
11.7
8
50
305
4300
2650
12.8
O.D. x Length (mm)
30 x 115
Corning 8300-50
30 x 134
Falcon/Corning Conical*
* With Thermo Electron 315 cushion, order adapter 1106 and cushion 571 to spin Falcon/Corning tubes
Rotor 818 12-Place Fixed Angle 35°
(Use requires 50968 tapered shaft adapter)
No. of
Places
12
12
12
12
Tube
Volume
(ml)
Adapter
Number
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
5
-
8500
6060
7.5
1.5 - 2
B-D/.5
5762
5761
8500
8500
4680
4770/3960
5.5
5.9/5.0
12 x 80 Thermo Electron 2804,
2840
11 x 66
8 x 66
.4/.25
5760
8500
4680/3630
5.9/5.0
6 x 66
OM3750 Rev. 5 11 January 2004
O.D. x Length (mm)
Page 14 of 24
Rotor 819 10-Place Fixed Angle 35°
(Use requires 50968 tapered shaft adapter)
No. of
Places
Tube
Volume
(ml)
Adapter
Number
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
10
-
8500
6060
7.6
10
O.D. x Length (mm)
16.1 x 85 Thermo Electron 2046, 2067, 2801,
2850
Rotor 836 6-Place Fixed Angle 30°
(Use requires 50968 tapered shaft adapter)
No. of
Places
Tube
Volume
(ml)
Adapter
Number
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
30
-
8500
6060
7.5
15
5965
8500
5820
7.2
25.8 x 100 Thermo Electron 2047, 2055,
2802
16.1 x 109
5
5966
8500
5650
7.0
12.1 x 112
3
5967
8500
5490
6.8
10.9 x 95
6
6
6
6
O.D. x Length (mm)
Rotor 841 12-Place Fixed Angle 45×
(Use requires 50968 tapered shaft adapter)
Tube
Volume
(ml)
Adapter
Number
Max
Speed
(rpm)
Max
RCF
(xg)
Max
Radius
(cm)
1.5 - 2
-
8500
4680
5.8
11 x 39
12
12
B-D
5763
8500
4770
5.9
B-D Microtainer Tubes
.5
5763
8500
3960
4.9
8 x 66
12
12
.4
5764
8500
4680
5.8
6 x 46
.25
5764
8500
3630
4.5
6 x 46
No. of
Places
12
O.D. x Length (mm)
Microtube Rotors
(Use requires 50036 hub adapter on 50968 tapered shaft adapter)
Adapter Cat.
No
Max RCF
(xg)
Radius (cm)
-
6740
8.35
5763**
6830
8.45
8500
6020
6740
5860
6740
6830
7.45
8.35
7.25
8.35
8.45
8500
6020
7.45
6740
5860
6460
8.35
7.25
8.00†
6060
7.50‡
6950
6790
8.60†
8.40
6060
7.50
6870
8.50
Rotor Cat. No.
No. of Tubes and Tube Size
24 x 1.5 ml
8911
(Aerosol
Contained)
24 x 0.6 ml B/D Microtainers™
24 x 0.5 ml PCR microtubes
24 x 0.4 ml microtubes
24 x 0.25 ml microtubes
24 x 1.5 ml
24 x 0.6 ml B/D Microtainers™
5763**
5764**
5764**
5763**
8512
24 x 0.5 ml PCR microtubes
5763**
24 x 0.4 ml microtubes
24 x 0.25 ml microtubes
48 x 0.5 ml PCR microtubes
5764**
5764**
-
24 x B/D Microtainers™
40 x 0.4 ml microtubes
-
40 x 0.25 ml microtubes
-
40 x 0.8 ml (6x50 mm) glass
-
8522
8532
Max Speed
(RPM)
8500
8500
Microtainers is a registered trademark of Becton Dickinson
** Order 2 pks of adapters separately. Thermo Electron 5763 and 5764 are packaged 12/pk.
†
Outer row holds 24 tubes
‡
Inner row holds 24 tubes
Thermo Electron Corporation’s 891 Rotor Provides Aerosol Containment and has been tested for microbiological containment by PHLSCAMR, Porton Down. Meets requirements of US OSHA Bloodborne Pathogen Final Rule: (Regulation 29 CFR Part 1910.1030.
Complete with Thermo Electron Corporation’s 50417 Aerosol Containment Cover, 50525 Inner and 36597 Outer rubber O-ring seals.
2
Thermo Electron Corporation’s 851, 852, 853 rotors
Page 15 of 24
OM3750 Rev. 5 11 January 2004
245 Horizontal Rotor 4x250 ml
(Use requires 50968 tapered shaft adapter)
Max Tube Size
O.D. mm
L. mm
No. of Tubes x Tube Size
40 x 1.5 ml microtubes
40 x 3 ml
48 x 5 ml
5-7 ml
(13 x 75-100mm vacutainers)
28 x 10-15 ml vacutainers
16 x 15 ml Falcon Conical
12 x 30 ml
4 x 50 ml
4 x 50 ml Falcon/Corning Conical
4 x 175 ml (Falcon 2076)
4 x 200 ml (Nunc 376813)
4 x 225 ml (Falcon 2075)
4 x 250 ml (Thermo Electron 2502, 2504)
Ultrac
Adapter
Max
RPM
Max
RCF
xg
Radius
(cm)
10.9
12.1
75
131
7228
7228
7226
2750
2750
2750
1375
1450
1450
16.2
17.2
17.2
13.3
131
7238
2750
1450
17.2
16.2
25.5
28.6
61.0
61.0
61.0
61.0
110
131
131
131
131
131
131
7225
7230
7223
7231
7231
*
**
*
-
2750
2750
2750
2750
2750
2750
2750
2750
2750
1450
1475
1450
1450
1450
1475
1475
1475
1500
17.2
17.6
17.2
17.2
17.2
17.6
17.6
17.6
17.8
* Also order Falcon 2090 cushions
**Also order Thermo Electron 5792 cushion
224/268 Horizontal Rotor 4x250 ml
(Use requires 50968 tapered shaft adapter)
Max Tube Size
O.D. mm
L. mm
No. of Tubes x Tube Size
40 x 1.5 ml microtubes
40 x 3 ml
48 x 5 ml
40 x 7 ml
28 x 7-10 ml
12 x 15 ml Falcon Conical
12 x 30 ml
4 x 50 ml
4 x 50 ml Falcon Conical
4 x 75 ml (Thermo Electron 2059)
4 x 175 ml (Falcon 2074)
4 x 250 ml (Thermo Electron 2261, 2051)
10.9
12.1
13.3
16.2
25.5
28.6
38.1
61.0
61.0
Ultrac
Adapter
Max
RPM
Max
RCF
xg
Radius
(cm
7228
7228
7226
7236
7225
7234
7223
7222
7231
7221
*
-
4000
4000
4000
4000
4000
4000
4000
4000
4000
4000
4000
4000
2450
2630
2630
2630
2630
2700
2630
2630
2630
2630
2700
2736
13.7
14.7
14.7
14.7
14.7
15.1
14.7
14.7
14.7
14.7
15.1
15.3
75
106
106
106
106
110
112
125
125
* Also order Falcon 2090 cushions
958 Horizontal Rotor 6x50 ml
(Use requires 50968 tapered shaft adapter)
No. of Tubes x Tube Size
48 x 5 ml
36 x Shell vial
36 x 7 ml
30 x 5 ml
24 x 7 ml
18 x 10 ml
24 x 10 ml
18 x 10 ml
6 x 15 ml
6 x 15 ml
12 x 50 ml
6 x 50 ml
6 x Microscope Slides
Page 16 of 24
Max Tube Size
O.D. mm
L. mm
13.6
17.7
17.7
12.6
14.2
16.5
17.2
17.2
17.2
17.2
30.0
30.0
1”
92
77
88
98
106
110
103
112
119
134
120
124
3”
TR.
Ring
Carrier or
Shield
Max
RPM
Max
RCF
xg
Radius
(cm)
366
366
366
354
355
310
310
326
325
-
381
379
380
369
1018
1013
356
356
356
303
320
320
1025
2600
2900
2600
3200
2800
2800
2400
2450
2950
2700
2400
2800
1800
1125
1300
1125
1600
1375
1400
1025
1075
1575
1475
1100
1475
500
15.0
13.7
15.0
14.0
15.7
15.9
16.1
16.1
16.1
17.6
16.9
16.8
13.5
OM3750 Rev. 5 11 January 2004
Hemato-Kit Rotor 930
(Use requires 1116 hublock adapter on 50968 tapered
shaft adapter)
The 930 Hemato-Kit can convert the IEC CL3 Series into a microhematocrit centrifuge, providing a simple, economical way to perform
occasional hematocrits. Blood cells are packed in about ten minutes, at
7800 RPM/6200 xg. Kit includes numbered 24-place rotor for 1.75 x 75
mm capillary tubes, 1505 gasket for tubes, cover, hublock adapter and
wrench
for installing rotor. An accessory microcapillary tube reader available, to
complete the system.
Order Cat. No. 930 Hemato-Kit and Cat. No. 2201 Microcapillary Tube
Reader.
4.3 Derating Tables for Dense Samples
The Speed and Force Tables list the maximum speed for
each rotor/adapter combination for the IEC CL3 Series.
These speeds are guaranteed, for samples whose specific
gravity is not greater than:
• 1.2 for swinging bucket rotors
• 1.5 for fixed angle rotors
For denser samples, the maximum guaranteed speed is
reduced (derated) by a factor from the table below:
Specific Gravity
Swinging Bucket
Fixed Angle
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
2.0
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
3.0
1.000
.960
.925
.894
.866
.839
.816
.794
.774
.755
.738
.721
.707
.692
.678
.666
.654
.642
.632
1.000
1.000
1.000
1.000
.967
.939
.912
.888
.866
.844
.825
.807
.790
.774
.758
.744
.731
.719
.707
! CAUTION
Do not exceed the rated speed or specific gravity.
Higher speeds or specific gravities will impose
unnecessary wear on the centrifuge, and can cause
rotor failure. Wear and damage caused in this manner
are not covered under warranty.
244 Rotor
The 244 rotor is designed for centrifugation of multi-well
microplates. The weight of the loaded microplates must be
equally distributed between the rotor’s two carriers. The
maximum rated speed for the 244 rotor, when each of the
carriers is loaded with 280 grams (total load of 560 grams),
is 4000 rpm.
! CAUTION
Loads greater than 280 grams (weight of the microplate
and sample) must have maximum speed derated
(according to the following table), to avoid rotor failure.
Derating Table for 244 Rotor
Load per Carrier
(grams)
Max. Speed
(rpm)
280
300
320
340
360
380
400
450
500
600
Greater than 600
4000
3600
3300
3000
2800
2600
2400
1900
1400
800
do not use
Derating Example: An angle rotor, rated for 10,000 RPM,
used with samples with a specific gravity of 1.6, cannot
spin faster than 9,670 RPM. (10,000 x .967 = 9.670)
Specific gravities greater than 3.0. This table is based on
the formula:
(S o / S a )
You can use the same formula to compute derating factors
for specific gravities greater than 3.0.
• So is the maximum specific gravity allowed before
derating (1.2 or 1.5, depending on the type of rotor.
• Sa is the actual specific gravity of the sample.
OM31150 Rev. 5 11 January 2004
Page 17 of 24
4.4 Chemical Resistance Table
The centrifuge, rotors and accessories are comprised of made of materials that are designed to resist attack from most
laboratory chemicals. For your convenience, the chemical resistance table below shows the suitability of various materials
with different classes of reagents.
NOTE: Refer to the cleaning section for information on cleaning and removing corrosion from various parts. Clean spills
promptly to minimize the effect of corrosive chemicals and avoid expensive repairs.
Plastic
N
N
F
E
E
E
E
E
N
E
N
N
F
N
E
E
G
N
N
N
N
N
N
E
E
F
E
N
G
N
E
N
N
N
F
E
E
G
G
E
N
N
E
E
F
N
E
E
PHENOLIC FIBER
F
N
E
E
E
E
E
E
E
G
N
F
VITON®
F
N
E
E
E
E
E
E
E
G
N
F
BUNA-N
G
N
E
E
E
E
E
E
E
G
F
F
RUBBER
G
N
E
E
E
E
E
E
E
E
E
E
MAGNESIUM
E
F
E
N
E
N
N
N
N
N
N
E
Other
MAGANESE BRONZE
F
N
N
F
F
E
E
E
G
E
N
E
ALUMINUM
N
N
E
G
G
G
E
E
E
E
N
E
STAINLESS STEEL
F
N
E
G
N
G
E
E
G
E
N
E
TITANIUM
E
N
E
G
G
E
N
N
N
N
N
E
POLYSTYRENE
G
F
F
G
N
N
E
N
N
N
N
E
NYLON
E
E
E
G
E
G
G
F
F
G
F
E
ACETAL COPOLYMER
(CELCON®)
ACETAL HOMOPOLYMER
(DELRIN®)
MODIFIED
PHENYLENE OXIDE (NORYL®)
POLYURETHANE
E
E
E
G
E
G
G
G
F
G
F
E
POLYPROPYLENE
E
N
G
F
N
N
F
N
N
N
N
E
POLYETHYLENE
E
E
E
G
E
G
G
F
F
G
F
E
POLYCARBONATE
POLYALLOMER
Acids, dilute or weak
Acids*, strong or conc.
Alcohols, aliphatic
Aldehydes
Bases
Esters
Hydrocarbons, aliphatic
Hydrocarbons, aromatic
Hydrocarbons, halogenated
Ketones
Oxidizing Agents, strong
Salts
Metal
E
N
E
E
N
E
E
E
E
E
E
E
*For Oxidizing Acids, see "Oxidizing Agents, strong”.
Classification
of Resistance
E = EXCELLENT
G = GOOD
F = FAIR
N = NOT RECOMMENDED
Page 18 of 24
OM3750 Rev. 5 11 January 2004
4.5 Decontamination Table
Compatible Processes For Decontamination
Sterilization Methods
Plastic
Metal
Other
POLYCARBONATE
POLYETHYLENE
POLYPROPYLENE
POLYURETHANE
MODIFIED
PHENYLENE OXIDE (NORYL)
ACETAL HOMOPOLYMER
(DELRIN)
ACETAL COPOLYMER
(CELCON)
NYLON
POLYSTYRENE
TITANIUM
STAINLESS STEEL
ALUMINUM
MAGNESIUM
RUBBER
BUNA-N
VITON
PHENOLIC FIBER
PT - PAINTED SURFACE
M
S
U
U
S
U
S
S
U
M
S
U
U
S
U
S
S
U
S
S
U
S
S
U
U
S
U
S
S
S
S
S
S
S
S
U
S
S
S
S
S
S
S
U
U
S
U
U
M
S
U
S
S
U
M
S
U
S
S
S
S
S
S
S
M
S
S
M
S
S
S
S
U
S
S
S
S
S
S
S
U
S
S
S
S
S
S
S
S
U
U
M
M
U
S
S
U
S
S
S
S
S
S
U
U
S
S
S
S
U
M
U
M
S
S
S
S
U
S
U
M
U
S
U
U
U
U
U
U
M
U
M
S
S
S
S
M
S
S
S
S
S
S
S
M
S
S
S
S
M
S
S
M
S
S
S
S
U
S
S
M
S
S
S
S
U
S
S
M
S
S
S
M
U
U
S
M
S
S
S
S
S
S
U
M
S
U
S
S
U
S
U
U
S
S
U
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
M
M
U
U
MAGANESE BRONZE
POLYALLOMER
S
S
U
Mechanical
Autoclave*
Ethylene Oxide Gas
Dry Heat (2Hrs. @ 160°C)
Chemical
Ethanol
40% Formalin
Methanol
2-Propanol
.5% Sodium Hypochlorite**
3% Hydrogen Peroxide
100% Hydrogen Peroxide
5% Phenol Solution
*For Oxidizing Acids, see "Oxidizing Agents, strong"
Autoclaving 121° C for 20 min. @ 2 ATM (15 PSIG)
** 1 to 10 Dilution of Household Bleach
S = SATISFACTORY
M - MARGINAL
U - UNSATISFACTORY
! WARNING
This chart describes the material compatibility of various sterilization methods. It does not specify the adequacy of
sterilization. Refer to the Chemical Resistance Table on the previous page for material compatibility during
centrifugation.
OM3750 Rev. 5 11 January 2004
Page 19 of 24
4.6 RCF Nomograph
Page 20 of 24
OM3750 Rev. 5 11 January 2004
5. Maintenance
5.3 Storage: Keep the Unit Dry
5.1 Care and Cleaning
Rotors and other parts should be clean and dry. Store them
open to the air, not in a plastic bag, so any residual
moisture evaporates. Face the parts upward to avoid
moisture retention in the cavities.
• Keep the centrifuge clean to ensure good operation and
to extend its life.
• Clean the sample chamber, rotor, and lid, at the end of
each work day, and immediately after any spill. Use a
damp sponge, warm water, and a mild liquid detergent,
suitable for washing dishes by hand, such as Ivory®
liquid. Do not use caustic detergents or detergents that
contain chlorine ions. These attack metals.
• Remove stubborn stains with a plastic scrub pad. Do
not use steel wool, wire brushes, abrasives, or
sandpaper as they create corrosion sites. Never pour
water directly into the rotor chamber.
• Scrub the rotor’s tube cavities with a stiff test tube
brush that has end bristles and a non-metallic tip. After
cleaning, dry each part with a clean absorbent towel.
! CAUTION
In the case of glass breakage, be careful to remove
ALL particles of glass from the unit! In particular, be
sure thoroughly scrub adapters and cushions with a
wire brush or replace these items as glass shards
embedded in the adapters can cause further
breakage.
5.2 Corrosion Cleaning Procedure
The rotors and structural accessories are finished to give
maximum resistance to corrosion. To maximize the life of
the unit, continually inspect the rotor cavities for corrosion,
especially if you use chloride ion solutions such as sodium
chloride (saline), and sodium hypochlorite (household
bleach), because these solutions attack most metals.
Clean the rotor, rotor chamber, and accessories
(particularly the sample compartments and bucket cups)
thoroughly after each exposure. Inspect all surfaces under
bright light for corrosion. Be aware that small crevices
grow deeper, eventually resulting in system failure.
If you see any corrosion, remove it immediately, using the
following procedure:
1. Follow the cleaning procedure at the start of this
section.
2. Soak the product in mild hand dish-washing detergent
and scrub the product thoroughly with a stiff test tube
brush. The brush should have end bristles and a nonmetallic tip.
3. Soak the product in clear warm water for a minimum of
an hour.
4. Rinse the product in warm water then in distilled water.
5. Dry the product thoroughly with a clean absorbent
cloth.
! CAUTION
If this procedure does not remove the corrosion,
discontinue use of the product and inform technical
service.
OM31150 Rev. 5 11 January 2004
Store parts on a soft surface to avoid damage.
5.4 Decontamination Procedures
! WARNING
If tube breakage occurs releasing toxic, infectious,
pathogenic, or radioactive material into the unit,
decontaminate the chamber.
Rotors have sealed containers that provide aerosol
containment and, if used as directed, keep spillage
confined. If breakage occurs it may be sufficient to only
decontaminate the sealed carriers.
The Decontamination Table in the previous section lists the
sensitivity of various materials to common sterilization
procedures. When using a 1-to-10 dilution of household
bleach (sodium hypochlorite) to decontaminate metal rotors
or accessories, follow decontamination by the corrosion
cleaning procedure (5.2) since chloride ions attack most
metals.
Always decontaminate for the minimum recommended
time. If you observe corrosion, remove it as described
earlier, discontinue use of the method and use an alternate
decontamination procedure.
Polypropylene sealed carriers can be autoclaved. Remove
any sample tubes before autoclaving unless they are
completely full of sample. Remove caps, stoppers, and
other tube closures before autoclaving to keep the tubes
from collapsing under pressure. Autoclave the rotor and
accessories at 121° C @ 15 psig for 20 minutes. Do not
stack polypropylene rotors during this process. After
cooling, perform a normal cleaning operation as described
above.
Repeated autoclaving seriously degrades the performance
of polycarbonate sealing covers.
5.5 Cover Interlock Bypass
The cover will remain locked if power fails. If you need to
remove samples from the unit before power is restored, use
the cover interlock bypass after the rotor has come to a
stop.To bypass the cover interlock:
1. Unplug the centrifuge.
2. Locate the hidden plastic plug underneath the front
ledge of the cabinet.
3. Use a screwdriver to pry out and remove the plug.
4. Pull the attached cord to release the cover interlock.
5. Replace the plug in the hole.
! WARNING
Do not perform this operation routinely. The
centrifuge’s cover interlock provides operator safety. It
allows the cover to be opened promptly, whenever
rotation has stopped.
Page 21 of 24
5.6 Condition of Returned Equipment
Obtain a return goods authorization (RGA) before returning
equipment to the manufacturer. The RGA paperwork
includes a Certificate of Decontamination for you to sign.
It indicates that you have performed the proper steps for
decontaminating the unit.
! WARNING
All returned units must be decontaminated, free of
radioactivity, and free of hazardous, infectious,
pathogenic, or toxic materials.
All return equipment shipments will be refused until the
signed certificate is received.
You must prepay transportation to the service depot.
6. Specifications
IEC Centra CL3
IEC Centra CL3R
8,500 RPM (841 fixed angle rotor)
4,000 RPM (243 swinging bucket rotor)
6,200 xg (930 fixed angle rotor)
3,200 xg (243 swinging bucket rotor)
48 x 5 ml sealed (243 rotor)
12 x 25 ml Universal sealed (243 rotor)
20 x 15 ml Falcon/Corning sealed (243 rotor)
8 x 50 ml Falcon/Corning sealed (243 rotor)
4 x 250 ml (243 rotor)
4 x Microplates/2 x Deep plates (244 rotor)
Maximum Speed:
Maximum Force:
Maximum Number of
Tubes:
Maximum Sample
Volume:
Sound Level:
1 liter (243 rotor)
60 db(A) max.
Operator Controls
Chamber
Temperature:
Speed:
Spin Duration:
-9° to 40° C by 1°
0 - 8,500 RPM, by 100 RPM
0:01 through 0:59, by 1 second
1:00 through 4:45, by 15 seconds5 through 99
min., by 1 min.
Momentary operation mode
Hold mode (indefinite duration)
At-Start timing mode
At-Speed timing mode
Repeatability
Temperature Control:
Rotation:
:± 1 C° in the range from
+4° C to +22° C
Accuracy within ±10 RPM
System Components
Motor:
Refrigerant
3/8 hp, Brushless DC
HP-80, CFC-free
Power Requirements and Output
Voltage
Operating Current:
Heat Output:
3750 - 120V/60 Hz
3751 - 220-240V/50/60 Hz
3755 - 120V/60 Hz
3756 - 220-240V/50 Hz
3757 - 220-240V/60 Hz
3750 - 5.5 AMPS
3751 - 3 AMPS
3755 - 10 AMPS
3756 - 5 AMPS
3757 - 5 AMPS
Full specification compliance is guaranteed for
up to ±5% line voltage specified. Function
guaranteed up to ±10% of line voltage
1300 BTU/Hr
2400 BTU/Hr
Dimensions
Sample Load Height:
32 cm (12.5 in)
Cover Closed Height:
37 cm (14.5 in)
Cover Open Height:
84 cm (33 in)
Width
68 cm (26.5 in)
49 cm (19.25 in)
Depth
53 cm (21 in)
Unit Weight:
68 kg (150 lbs)
46 kg (100 lbs)
Shipping Weight:
91 kg (200 lbs)
68 kg (150 lbs)
Specifications subject to change without notice.
Page 22 of 24
OM3750 Rev. 5 11 January 2004
Warranty
Thermo warrants that the Products will operate
substantially in conformance with Thermo’s Specifications
applicable to such Products, when subjected to normal,
proper and intended usage by properly trained personnel,
for a period of twenty-four (24) months from the date of
installation, not to exceed thirty (30) months from date of
shipment from Thermo (the “Warranty Period”). Thermo
agrees during the applicable Warranty Period, provided it is
promptly notified in writing upon the discovery of any
defect and further provided that all costs of returning the
defective Products to Thermo are pre-paid by Purchaser, to
repair or replace, at Thermo's option, non-conforming
Products so as to cause the same to operate in substantial
conformance with said Specifications. Such repair shall
include parts only during the final twelve (12) months of
the Warranty Period. Replacement parts may be new or
refurbished, at the election of Thermo. All replaced parts
shall become the property of Thermo. Lamps, fuses, bulbs
and other expendable items are expressly excluded from
the warranty. Thermo's sole liability with respect to
equipment, materials, parts or software furnished to
Thermo by third party suppliers shall be limited to the
assignment by Thermo to Purchaser of any such third party
supplier's warranty, to the extent the same is assignable.
In no event shall Thermo have any obligation to make
repairs, replacements or corrections required, in whole or in
part, as the result of (i) normal wear and tear, (ii) accident,
disaster or event of force majeure, (iii) misuse, fault or
negligence of or by Purchaser, (iv) use of the Products in a
manner for which they were not designed, (v) causes
external to the Products such as, but not limited to, power
failure or electrical power surges, (vi) improper storage of
the Products or (vii) use of the Products in combination
with equipment or software not supplied by Thermo. If
Thermo determines that Products for which Purchaser has
requested warranty services are not covered by the
warranty hereunder, Purchaser shall pay or reimburse
Thermo for all costs of investigating and responding to
such request at Thermo's then prevailing time and
materials rates. If Thermo provides repair services or
replacement parts that are not covered by the warranty,
Purchaser shall pay Thermo therefore at Thermo's then
prevailing time and materials rates. ANY INSTALLATION,
MAINTENANCE, REPAIR, SERVICE, RELOCATION OR
ALTERATION TO OR OF, OR OTHER TAMPERING WITH,
THE PRODUCTS PERFORMED BY ANY PERSON OR
ENTITY OTHER THAN THERMO WITHOUT THERMO'S
PRIOR WRITTEN APPROVAL, OR ANY USE OF
REPLACEMENT PARTS NOT SUPPLIED BY THERMO,
SHALL IMMEDIATELY VOID AND CANCEL ALL
WARRANTIES WITH RESPECT TO THE AFFECTED
PRODUCTS.
THE OBLIGATIONS CREATED BY THIS SECTION TO
REPAIR OR REPLACE A NON-CONFORMING PRODUCT
SHALL BE THE SOLE REMEDY OF PURCHASER IN THE
EVENT OF A NON-CONFORMING PRODUCT. EXCEPT AS
EXPRESSLY PROVIDED IN THIS WARRANTY, THERMO
DISCLAIMS ALL WARRANTIES, WHETHER EXPRESS OR
IMPLIED, ORAL OR WRITTEN, WITH RESPECT TO THE
PRODUCTS, DELIVERABLES AND SERVICES, INCLUDING
WITHOUT LIMITATION ALL IMPLIED WARRANTIES OF
NON-INFRINGEMENT, MERCHANTABILITY OR FITNESS
FOR ANY PARTICULAR PURPOSE. THERMO DOES NOT
WARRANT THAT THE PRODUCTS, DELIVERABLES AND
SERVICES, ARE ERROR-FREE, OR WILL ACCOMPLISH
ANY PARTICULAR RESULT. FURTHER, WITH RESPECT
TO ANY SOFTWARE PRODUCT OR LICENCED
SOFTWARE, PROVIDED TO PURCHASER HEREUNDER,
THERMO MAKES NO REPRESENTATIONS OR WARRANTY
THAT ALL ERRORS HAVE BEEN OR CAN BE ELIMINATED
FROM THE SOFTWARE, THAT IT WILL OPERATE
WITHOUT INTERRUPTION OR THAT IT WILL OPERATE
WITH OTHER PRODUCTS. THERMO EXPRESSLY
DISCLAIMS ANY WARRANTY WHATSOEVER WITH
RESPECT TO ANY OTHER THIRD PARTY PRODUCTS
WHETHER LICENSED OR SOLD TO PURCHASER
DIRECTLY BY A THIRD PARTY VENDOR OR SUBLICENSED
OR RESOLD TO PURCHASER BY THERMO. Thermo
warrants that the Services provided hereunder will comply
with the requirements expressly set forth in the terms and
conditions set forth in this warranty hereto and will
otherwise be performed in accordance with generally
accepted industry practice by competent personnel. In the
event that any Services fail to comply with the foregoing
standard, Thermo will, at its option, provided it is promptly
notified in writing upon discovery of such failure, either
repeat such non-complying Services at no additional charge
or refund to Purchaser all fees theretofore paid by
Purchaser with respect to such non-complying Services.
Notwithstanding the foregoing, the cost of any such repeat
of Services which generates results consistent with the
original results will be at Purchaser’s expense. THE
FOREGOING WARRANTY IS THE SOLE AND EXCLUSIVE
WARRANTY GIVEN BY THERMO IN CONNECTION WITH
THE SERVICES PERFORMED HEREUNDER, AND IS IN LIEU
OF ALL OTHER WARRANTIES OF ANY KIND, WHETHER
EXPRESS OR IMPLIED, ORAL OR WRITTEN.”
Copyright © 2004 by Thermo Electron Corporation
All rights reserved. No part of this work may be reproduced or transmitted in any form
or by any means, electronic or mechanical, including photocopying and recording, or
by any information storage or retrieval system, except as may be expressly permitted
in writing by Thermo Electron Corporation. Printed in the United States of America.
OM31150 Rev. 5 11 January 2004
www.thermo.com
Page 23 of 24
Thermo Electron Corporation
Bioscience Technologies
450 Fortune Boulevard
Milford, MA 01757
866.9.THERMO (866.984.3766)
www.thermo.com
Fax: 508.634.2199
Laboratory Pipetting and Consumables
Molecular Biology
info.pipettes@thermo.com
info.molbio@thermo.com
Microplate Instrumentation
Sample Preparation
info.microplateinstruments@thermo.com
info.sampleprep@thermo.com
Laboratory Automation & Integration
New Labs
info.labautomation@thermo.com
newlabs@thermo.com
Controlled Environment
Services
info.controlenv@thermo.com
services.biosciencetech@thermo.com
Bioscience
Technologies
International Sales Office Locations
Belgium
Brussels
+32 2 482 30 30
Fax: +32 2 482 30 31
France
Cergy Pontoise Cedex
+33 1 34 32 51 51
Fax: +33 1 34 32 51 59
Germany
Dreieich
+49 6103 408 0
Fax: +49 6103 408 1222
Netherlands
Breda
+31 76 571 4440
Fax: +31 76 587 9757
Russia
Saint-Petersburg
+7 812 325 8045
Fax: +7 812 186 1194
Moscow
+7 095 755 9045
Fax: +7 095 755 9046
China
Spain
Beijing
+8610 5850 3588
Fax: +8610 6621 0847
Barcelona
+34 93 2233154
Fax: +34 93 2230857
Shanghai
+8621 5465 7588
Fax: +8621 6445 7830
Sweden
Hong Kong
Stockholm
+46 8 742 03 90
Fax: +46 8 742 09 47
Wanchai
+852 2885 4613
Fax: +852 2567 4447
Lund
+46 46 90 96 60
Fax: +46 46 32 87 70
India
United Kingdom
Basingstoke, Hampshire
+44 01256 81782
Fax: +44 01256 81792
Bangaalore
+91 22 2778 1101
Fax: +91 22 2778 1103
Japan
Yokohama-City
+81 45 453 9122
Fax: +81 45 453 9222
© 2003-2004 Thermo Electron Corporation. All rights reserved.
Thermo Electron Corporation, and Analyze, Detect,
Measure, Control are trademarks of Thermo Electron Corporation.
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertising