Sartorious Professional Meter PP-20

Sartorious Professional Meter PP-20
Operating Manual
Sartorius
Professional Meter
PP-15, PP-20, PP-25, PP-50
98648-013-07
Contents
4
Warning and Safety Information
5
Quick Start Guide
for pH Measurement
Introduction
Direct Menu Keys, Soft Keys
and Display
Electrode Connectors and Inputs
LCD
Function Keys
7
8
8
10
12
37
Quick Start Guide for
Conductivity/Resistivity/Salinity/TDS
Measurements
38
Conductivity/Resistivity/Salinity/TDS
Menus
38
Conductivity/Resistivity/Salinity/
TDS Standardize Menu
39
Conductivity Mode Options Menu
41
Conductivity Measurements
43
Data Logging
45
Troubleshooting
46
Technical Specifications
17
17
18
18
18
Electrodes
Preparing pH Electrodes
and Conductivity Cells
Using and Storing Electrodes
pH Glass Electrodes
Connecting Electrodes
Ion Selective Electrodes
Conductivity Cells
19
Meter Setup Menu
47
pH Theory
21
21
21
22
22
25
26
pH Mode
pH Mode Standardization Menu
Cal Reminder Menu
Select Custom Buffer Set
pH Mode Options Menu
Standardizing and Measuring pH
Clearing Buffers
48
Ion Selective Electrode Theory
49
Determining the Isopotential Point
50
RS-232 Serial Interface Meter
Command Set
53
Pin Assignment
27
27
27
28
mV Mode
mV Mode Standardization Menu
mV Mode Options Menu
Clearing Relative mV Mode
54
Maintenance
55
Menu Tree Diagram
29
Quick Start Guide
for Ion Measurements
Ion Mode
Standardizing and Measuring Ion
Measuring Ion Using Known Addition Type (Incremental Ion) Methods
57
Accessories
58
C Marking
16
16
31
33
35
3
Warning and Safety Information
For safety and operating reasons, only
authorized service technicians may open
the Professional Meter Basic housing.
Therefore, only authorized technicians may
repair or perform maintenance on this pH
meter. Any tampering with the pH meter
or negligent or intentional damage to this
equipment will void any warranty claims
against the manufacturer.
If liquid gets into the Professional Meter,
unplug it from AC power (main supply)
and have an authorized service technician
check the pH meter.
If you do not plan to use this Professional
Meter for a relatively long period, please
disconnect it from AC power.
For safety reasons, use this equipment
only for the application described in this
operation manual.
Make sure that the buffers used for
standardizing have exactly the same values
that are stored.
The meter allows 5 automatic standardization buffers in pH and Conductivity modes,
and 7 in Ion mode. When you enter a
buffer in addition to the number allowed,
the buffer farthest away from the average
is replaced by the new buffer pH.
4
Information
on Radio Frequency Interference
Warning:
This equipment generates, uses and can
radiate radio frequency energy and, if not
installed and used in accordance with the
instruction manual, may cause interference
to radio communications. It has been tested and found to comply with the limits for
a Class A computing device pursuant to
Subpart J of Part 15 of FCC Rules, which
are designed to provide reasonable protection against such interference, when operated in a commercial environment. Operation of this equipment in a residential area
is likely to cause interference, in which case
the user, at his own expense, will be
required to take whatever measures may be
required to correct the interference.
Quick Start Guide
for pH Measurement
1. Connect power cable to meter connector on the rear
panel marked with the symbol “
” and to AC
power source.
“power”
“Temp A”
“Ch.A”
2. Connect the glass pH/ATC electrode to the channel
A BNC connector marked “Ch.A” and to the channel
A temperature connector marked “Temp A.”
3. To switch the meter on or off, press the I/O button
on the back of the meter.
5
channel indicator
stability indicator
7.000
▼ ▼
A s
pH
4. Verify that the meter is in pH mode on channel A.
Use the Mode and Channel keys to set the meter to
the correct mode and channel, if necessary.
(See Function keys).
▲
25.0°C
No buffers.
units indicator
Mode
Channel
Channel A: pH mode
Standardize Menu
1) Auto-enter a buffer
2) Manual buffer entry
3) Clear buffers
4) Options menu
5) Cal reminder menu
6) Select buffer set
5. Standardize the electrode by immersing the electrode in a buffer, pressing Standardize, pressing
1) Auto-enter a buffer and following the prompts.
Repeat this step to enter each buffer.
The meter will check the electrode and buffers,
and give an error message if there is a problem.
In addition to “Auto-enter a buffer”, the Standardize
Menu offers further functions.
Under menu item 4) Options menu, you can select
the resolution and other parameters for the current
mode and channel.
Standardize
Cal Data
A
s
7.000
25.0°C
4.008 pH
7.000
▲
entered buffers
6
pH
6. The display shows the current measurement,
and indicates a stable reading with the indicator S.
Press Cal Data to review and graph the electrode
calibration data.
Introduction
This electrochemistry meter is a powerful, versatile and accurate instrument.
It features easy menu-based operation with easy to understand prompts and electrode/
standard error checking.
These meters feature many advanced options, such as programmable stability criteria,
programmable standardization reading delay times, multi-channel operation, fast reading
update rates of twice per second for all channels, programmable alarms, programmable data
logging of up to 620 data points and a superb RS-232 bidirectional serial interface for
controlling the meter and obtaining data.
Soft Keys
Profes
Direct Menu Keys
Numeric Keypad
sional
Meter
PP-50
Direct Menu Keys
7
Direct Menu Keys, Soft keys and Display
The Sartorius Professional Meter uses six Direct Menu keys to access the menus and
operations (such as selecting pH mode, standardizing, checking electrode calibration data,
selecting the electrode channel).
There are four soft keys that provide additional operations; these soft keys change their
function as needed and each soft key has an icon to indicate its current function.
The display is a backlit quarter-VGA screen capable of displaying all three electrode channels
of the Model PP-50 simultaneously.
Electrode Connectors and Inputs
BNC (“Ch.A” or “Ch.B”) connectors: pH, Ion Selective Electrodes and ORP (redox) electrodes
attach to the meter through a BNC (“round twist-on”) connector to Channel A or Channel B
(Models PP-25 and PP-50 only).
Temperature (“Temp. A” or “Temp. B”) connectors: use to connect the 2.5-mm mini-phone
plug from the temperature sensor (built into the pH electrode with the standard pH/ATC
electrode) for Automatic Temperature Compensation (ATC).
Reference (“Ref.A” or “Ref.B”) connectors: use for attaching a reference electrode tip-pin
plug when a separate reference electrode is used.
Conductivity DIN (“Ch.C conductivity”) connector: use to connect a 4-band
conductivity/ATC cell. These 4-band cells offer improved linearity and stability over older
2-band conductivity cells. (Models PP-20 and PP-50 only).
Serial port (“RS-232”) DB-9 connector: use to connect a serial printer or a PC (personal
computer). This bidirectional interface outputs data and receives meter commands.
8
Power (“Power”) connector: use to connect a 5.5 mm coaxial connector with
12 VDC at 500 mA (center pin negative).
Note:
The connectors shown are not available on all models.
Connectors for Model PP-50
9
LCD
Single channel display
A B
K
J
I
H
G
*A
s
7.000
25.0°C
pH
C
CAL!
4.008 pH
7.000
9.999
LOG
6/30/1998
F
02:28 PM
E
D
Note: Not all of the following will be displayed at the same time.
A Result: current measurement.
B Units: displays the units for the current measurement.
Examples: pH, mV, mg/L F¯, µS/cm or O · cm.
C Soft key icons: show the current function assigned to each soft key.
D Calibration due reminder: the
CAL! icon reminds you a calibration is now due.
E Date and time: displayable in different formats.
F Data logging: the icon
LOG indicates data logging is active.
G Buffers/Standards: in single channel mode, all entered buffers or standards are displayed.
A “!” symbol next to a buffer indicates that the buffer is out of the entered calibration
valid time (See Calibration reminder, page 21).
10
H Temperature: displays the measured temperature
when an electrode with ATC or a separate temperature probe is attached. Shows “M” when a manually
entered temperature is being used.
I Alarm: “!” indicator means data is outside the set
alarm limits.
J Channel: indicates which electrode channel (input)
is being displayed. Channels A & B are BNC electrode
inputs, and Channel C is a conductivity cell input.
Multiple-channel display
L
K Stability: the
S indicates the electrode is stable to
the selected criteria.
L Multiple-Channel: display can show one
A
s
6.998
pH
(model PP-15), two (models PP-20 and PP-25) or
three (model PP-50) electrode measurements with
temperature simultaneously.
24.6°C
B
s
1.02
mg/L
23.4°C
F-
30-6-1998
14:28:05
M
M Out-of-range or non-valid reading: dashes indicate
A
pH
23.8°C
1998.6.30
that a measurement is not available.
This usually means that:
– the reading is out of range, or
– no standards have been entered in ion mode, or
– strict calibration has been set and the calibration
has expired.
14:28:30
11
Function Keys
?
I
E
A
Mode
Setup
Standardize
Data Log
Cal Data
Clear
Channel
Enter/Print
F
B
C
G
H
D
J
A Mode: selects the mode: pH, mV (Model PP-15), Ion (Models PP-25 and PP-50),
Conductivity – Resistivity – NaCl Salinity – Practical Salinity-TDS (Models PP-20 and
PP-50) to use for the currently selected channel.
B Standardize: enters buffers or standards for the currently selected channel.
Use to enter pH buffers, mV offset, ion standards or conductivity/resistivity standards.
Also used to change other settings which affect the measurement.
C Cal Data: displays and graphs buffers or standards with time and date stamp and
electrode calibration data for the selected channel and mode.
D Channel: selects the channel(s) (electrode inputs) to display.
Model PP-15 can display one channel (Channel A).
The Model PP-20 can display two channels (Channels A and C).
Model PP-25 can also display two channels (Channels A and B).
The Model PP-50 can display up to three channels simultaneously (Channels A, B and C).
12
E Setup: the Setup menu is used to set various general meter settings, such as date and
time, display contrast and serial port configuration.
F Data Log: displays the data logging menu used to set data logging and view the stored
Data Log (see Data Logging).
G Clear: exits the current menu and returns to the previous menu, cancels the current
operation or clears a number entry.
H Enter/Print: accepts numeric values, menu selections or pending operations.
In the main measure screen, it acts as a Print key, and stores the measurements in the
Data Log. All current measurements are sent to a printer/computer through the serial
port.
I Soft keys: these four keys access different operations at different times.
Most menus offer a “Help” soft key and the “Measure” soft key, which allows a direct
return to the main measuring screen, exiting all menus immediately. The “Up Arrow” and
“Down Arrow” soft keys offer one way to select a menu item. The “Left Arrow” key is a
backspace, which is active during number entry.
J Numeric Keys: pressing a number key selects a numbered item in a menu. The number
keys also allow entering values for buffer solutions, standards, and various meter settings.
13
Soft Keys
Help
Measure
Scroll up
Scroll down
Backspace
Graph
E
–
+
Exponent number entry
Incremental ion method
Press soft key: starts measurement
Measure lock: each display reading will freeze when stable
Measurement is performed
Channels
The Channel key is used to turn on or off each available channel. In single-channel
operation, additional information is provided for the selected channel, including a list
of all entered buffers or standards. In multi-channel operation, the Mode, Standardize and
Cal Data menus ask for the channel before accessing the menu.
Model PP-15:
Offers single-channel operation (pH and ORP electrodes).
Model PP-20:
Allows simultaneous dual-channel measurements using Channel A (pH glass membrane and
redox/ORP electrodes) and Channel C (conductivity cells).
14
Model PP-50
Select Channel Screen
Model PP-25:
Offers two-channel simultaneous operation of channel
A and channel B (pH, ORP and Ion Selective Electrodes).
Configure Display
1) Turn channel A on/off: ON
2) Turn channel B on/off: ON
3) Turn channel C on/off: ON
4) Measurement screen
Model PP-50:
Provides up to three channel measurements with
Channel A and Channel B (pH and ORP and ion
selective electrodes), Channel C (conductivity cells).
Channel
15
Electrodes
The meter allows you to use a variety of glass
membrane (“glass”) pH/ATC electrodes, ion selective
electrodes, conductivity/ATC cells, a separate temperature (ATC) probe, combination electrodes using
a BNC connector, or separate electrode pairs with
BNC connector and reference pin.
To measure
pH (glass membrane)
mV (ORP)
Ion (ISE)
Conductivity/Resistivity/
Salinity/TDS
Use channel
(connector)
A (BNC)* or B (BNC)*
A (BNC)* or B (BNC)*
A (BNC)* or B (BNC)*
C (DIN)
* Separate reference electrodes can be used with “Ref.
A” or “Ref. B” connectors.
Preparing Electrodes and Conductivity Cells
Remove the wetting cap or storage cap from the
electrode. Before first using your pH electrode or
whenever the electrode is dry, soak it several hours
in an electrode filling or storage solution (3 molar
KCl solution). Condition ISEs in the recommended
solutions. Rinse conductivity cells with deionized
water before use.
16
Using and Storing Electrodes
Provide
moderate
stirring
pH Glass Electrodes
§ Provide moderate stirring for faster electrode
response.
§ Leave the fill hole open during all use.
§ Rinse the electrode between each measurement
with a portion of the next sample or buffer to be
measured, or with deionized or distilled water.
Store with wetting cap
on in filling solution
(3 M KCl)
§ Keep glass electrodes wet when not being used
by placing some electrode filling solution in the
wetting cap and storing with the wetting cap on.
§ Keeping glass electrodes “wet” will improve their
performance. Store electrodes in electrode filling
solution or storage solution (KCl, 3 mol/L).
Conductivity Cells
§ Store conductivity cells dry.
17
ATC
Connector
BNC
Connector
Connecting Electrodes
pH, ORP or ISE electrodes (with BNC connector):
Connect the electrode to the BNC input, either
channel A or channel B (models PP-25 and PP-50
only), located at the rear of the meter. Push in and
rotate the electrode’s BNC connector until it locks in
place. Plug the ATC connector into the Temp. A or
Temp. B connector. To disconnect, twist the BNC
connector in the opposite direction and pull.
Electrode Pair Using a separate
Reference Electrode (with Reference Pin Plug):
Connect the indicating electrode to the BNC input.
Connect the reference electrode to the Reference
input. Push the electrode’s tip pin plug into the
input to connect and pull out to disconnect.
Channel C
Conductivity Input
Conductivity Cells (with DIN connector):
Align and push in the DIN connector fully to the
channel C input (models PP-20 and PP-50 only).
Pull carefully to disconnect.
Ion Selective Electrodes
§ Add proper amount of Ionic Strength Adjuster (ISA)
to all standards and samples, usually 1 mL ISA to
50 mL standard or sample.
§ Provide moderate stirring for faster electrode
response.
§ Rinse the electrode(s) between each measurement
with a portion of the next sample or standard to be
measured, or with deionized or distilled water.
§ Follow the instruction sheets for the individual
electrode. Store as recommended.
Conductivity Cells
§ When changing samples or standards, immerse
the cell into the new solution, then lift and allow
solution to drain out. Repeat two more times.
§ Gently tap cell to dislodge air bubbles.
18
Meter Setup
Meter Setup Menu
1) Time and date menu
2) Select temperature units
3) Select contrast
4) Language
5) Setup serial port
6) Keypress beep on/off
7) Select display background
8) Show meter information
9) Enable measure lock
0) Enable strict calibration
±) Set screen saver timeout
•) Restore factory defaults
Meter Setup Menu
Press Setup to access the Meter Setup menu:
1. Time and date menu: use to set the time format
(HH:MM:SS or HH:MM:AM/PM), the time, the date
format (MM/DD/YYYY, DD-MM-YYYY or
YYYY.MM.DD), and the date.
2. Select temperature units: use to select temperature
measurement and display in Celsius, Fahrenheit or
Kelvin.
Setup
Select Time Format
1) hr : min : sec
2) hr : min AM/PM
3. Select contrast: use to select the display contrast,
making the displayed characters lighter or darker.
Select setting “5” for typical conditions.
4. Select language: use to select the desired user
language.
5. Setup serial port: use to configure the serial port
start bits, baud rate and parity setting. This must
match the setting of the printer or computer being
used with the meter.
Select Date Format
6. Keypress beep: This menu item turns the keypress
beep ON and OFF.
1) mm / dd / yyyy
2) dd – mm – yyyy
3) yyyy.mm.dd
19
7.
Select display background: use to set the display
to black characters on a white background or white
characters on a black background.
If “measure lock” is enabled,
a “lock” soft key will appear on the
measurement screen.
8.
After pressing this key, each
reading will freeze when stable,
and an “L” icon will appear. To unlock
the readings, press the key again,
or exit the screen.
Note: logged data will not lock.
Show meter information: use to show the meter
model, software version and serial number.
9.
Enable measure lock: this feature locks (or freezes)
stable measurements for later review.
Stability criteria should be set to SLOW for all
channels and modes in use (see Options Menu).
Enable Measure Lock
Display lock/unlock soft key?
1) Yes
2) No
Enable Strict Calibration
Require calibration?
1) Yes
2) No
10. Enable strict calibration: use to set strict calibration where no measurements are displayed if the
calibration reminder has expired.
“±” Set screen saver: use to activate the screensaver.
If strict calibration is enabled,
no measurements will be
displayed for a channel with
an expired cal reminder.
“•” Restore factory defaults: use to reset all settings
to factory defaults. On occasion, it may be useful
to completely reset the meter, for example, if other
users have changed a setting.
Note: enabling this setting
locks all cal reminders ON.
!
20
Warning!
A reset also clears all electrode standardizations.
pH Mode
Channel A: pH mode
Standardize Menu
1) Auto-enter a buffer
2) Manual buffer entry
3) Clear buffers
4) Options menu
5) Cal reminder menu
6) Select buffer set
pH Mode Standardization Menu
Press Mode and select 1) pH.
Press Standardize and the pH Mode Standardize
Menu appears:
1. Auto-enter a buffer: use to add a new buffer
which is auto-recognized by the meter, or update an
existing buffer. Follow the prompts.
Standardize
Channel A: pH mode
Manual Buffer Entry
Enter the actual pH of this
buffer, after correcting
for temperature:
pH
Press Enter to accept.
Channel A: pH mode
Cal Reminder Menu
1) Turn reminder On/Off: OFF
2) Set reminder interval:
01, 00 : 00 days, hr : min
3) Standardization menu
When the cal reminder expires,
a “CAL!” (or “!”) icon will be
displayed with the measurement and
with each expired standard. If strict
cal is ON, reminders are always ON,
and expired channels show no
measurements.
2. Manual buffer entry: use to enter a buffer value by
manually entering the pH of the buffer.
3. Clear buffers: use to clear all buffers entered for the
current channel (pH mode). If all entered buffers are
being re-entered, it is usually not necessary to clear
buffers before re-entering them.
4. Options Menu: use to define additional specific pH
mode settings e.g. to set pH limits. (See page 23).
5. Cal reminder menu: use to set a timer reminding
you to recalibrate. A CAL! icon will appear on the
main screen and an exclamation mark beside the
buffers for which the time has expired.
The calibration reminder is a reminder of
when electrode calibration (with buffers) should
be performed again.
If strict calibration is set (see Meter Setup Menu)
and when a calibration has expired, the CAL! icon
appears, and “- - - ” is displayed in place of the
measurement. No measurements can be obtained
until a calibration is performed.
21
Channel A: pH mode
Select Buffer Set
1) 25°C: 1.68, 3.56, 4.01, 6.86,
7.42, 9.18, 10.01, 12.46
2) 25°C: 4.00, 7.00, 10.00
3) 25°C: 2.00, 4.00, 7.00,
10.00, 12.00
4) 20°C: 2.00, 4.00, 7.00,
10.00, 12.00
5) 20°C: 1.00, 3.00, 6.00,
8.00, 10.00, 6.00
6) 25°C: 1.09, 3.06, 4.65,
6.79, 9.23, 12.75
7) Select custom buffer set
No buffers
Channel A: pH mode
Configure Custom Buffer Set
1) Add a built-in buffer
2) Add a manual buffer
3) Delete a buffer
4) Standardize menu
Custom Buffer Set
No buffers
Channel A: pH mode
Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set pH slope
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Set isopotential point
0) Select temperature probe type
22
Note: When strict calibration is set, the calibration
reminders for all channels are turned ON, and
cannot be turned off from the Cal Reminder Menu.
6. Select buffer set: There are six auto-recognition
buffer sets and the option (7) to configure and use
a custom buffer set of your own.
7. Select Custom Buffer Set
Use Custom Buffer Set to make a set of buffers
containing the specific buffers in use (up to five
buffers). Select Custom Buffer Set and configure
the custom buffer set; then buffers from this set will
be automatically recognized and entered.
Custom buffers can have any numeric pH value,
or can be selected from the built-in temperaturecorrected buffers. Using the built-in buffers allows
temperature correction of the buffer pH values,
offering more accuracy.
pH Mode Options Menu
1. Select resolution: use to set pH readings to 0.1,
0.01, or 0.001 pH units.
2. Select stability criteria: use to set stability criteria
to slow, medium or fast to match the electrode’s
speed of response and the variability of the signal
allowed for a “stable” ( S ) measurement.
3. Select signal averaging: use to set filtering of the
electrode signal to very slow (10 readings), slow (8),
medium (6), fast (4) or very fast (2). Slower settings
produce more stable readings, althrough they make
take longer to reach stability.
4. Set standardization delay: use to set a reading delay time for the meter to wait before
accepting an electrode signal during standardization. Programming a standardization
reading delay helps slow responding electrodes reach equilibrium before the electrode
signal is accepted.
5. Set pH slope: use to set a known electrode slope utilized by the meter with a zero- or single-point standardization. The normal default slope is 59.16 mV/pH. The meter allows a
slope between 80 and 120% efficiency to be entered.
6. Standardize menu: returns to the pH mode Standardization Menu.
Caution: Either enter the slope for 25°C or work without the ATC probe. All manually
entered slopes are compensated according to the measuring temperature.
7. Manual temperature menu: use to set a temperature to be used in the absence of an
ATC probe or when manually overriding the ATC.
8. Data alarm menu: use to set pH limits.
If the limits are exceeded, an alarm indication “!” is displayed and recorded with any
data points placed in the Data Log.
9. Set isopotential point: use to set an isopotential point for use in high accuracy electrode
measurements (See Determining the Isopotential Point, page 47).
! Enter the isopotential point obtained during standardization.
0. Select temperature probe type:
1) Auto-Detect
20°C…40°C (66°F…104°F, 293 K…313 K)
Auto-Detect will work only if the temperature is between 20°C and 40°C when the
sensor is connected to the meter.
2) NTC 10 k O
3) NTC 30 k O
4) PT1000
Sartorius uses an NTC 10 k O-sensor as standard equipment.
23
Notes:
1. Auto-recognized buffers are found in the auto-recognized built-in buffer sets.
These buffers are auto-recognized by the meter, and are also automatically temperaturecorrected for the variation of buffer pH with temperature.
2. When manually entering buffers, the exact pH of the buffer at the current temperature
must be entered. All buffers change pH with temperature. For best accuracy, either
use the built-in buffers or make sure manually entered buffers are at the expected
temperature (so that their pH as entered is correct).
3. Auto-recognition
Buffer Sets:
1) 25°C: 1.68, 3.56, 4.01, 6.86,
7.42, 9.18, 10.01, 12.46
2) 25°C: 4.00, 7.00, 10.00
3) 25°C: 2.00, 4.00, 7.00,
10.00, 12.00
4) 20°C: 2.00, 4.00, 7.00,
10.00, 12.00
5) 20°C: 1.00, 3.00, 6.00,
8.00, 10.00, 6.00
6) 25°C: 1.09, 3.06, 4.65,
6.79, 9.23 12.75
7) Select custom buffer set
No buffers
4. Temperature Correction of Electrodes and Buffers
The meter automatically compensates for the temperature dependence of the electrode’s
response when measuring pH. The meter also compensates for buffer change in pH value
with temperature. Temperature compensation is based on temperature either from an
ATC probe or a manually entered temperature.
Actual Buffer pH vs. Temperature
pH 4.00 (4.01)/7.00/10.00 buffer (nominal 25°C)
Temperature (°C)
30
25
20
15
10
24
Buffer 4
4.016
4.008
4.003
4.000
3.998
Buffer 7
6.991
7.003
7.020
7.042
7.069
Buffer 10
9.947
10.000
10.057
10.119
10.187
Standardizing
and Measuring pH
Channel A: pH mode
Standardize Menu
1) Auto-enter a buffer
2) Manual buffer entry
3) Clear buffers
4) Options menu
5) Cal reminder menu
6) Select buffer set
1. Immerse the electrode in a buffer and stir moderately.
The meter displays the current pH measurement.
2. Allow the electrode sufficient time to reach
equilibrium.
3. Press Standardize, then press either
1) Auto-enter a buffer or
2) Manual buffer entry.
4. Follow the prompts on the display.
Standardize
5. The meter waits for a stable signal, automatically
recognizes the buffer (if using “Auto-enter”),
checks the electrode and buffer and enters
the buffer.
The entered buffer appears on the display.
Channel A: pH mode
pH Checklist
Prepare buffer or sample.
Rinse electrode.
Place electrode in solution.
Stir moderately.
Press Enter to continue.
6. Alternatively, if the signal is not stable, you can
press Enter when the reading stabilizes according
to your tolerance criteria. The meter then enters the
buffer.
Channel A: pH mode
Manual Buffer Entry
Enter the actual pH of this
buffer, after correcting
for temperature:
pH
Press Enter to accept.
7. Repeat steps 1 through 4 to enter a second, third,
fourth or fifth buffer. With more than one buffer,
the meter performs a diagnostic check on the electrode. The electrode is considered good if the slope
is between 90 to 105%. If a sixth buffer is entered,
the buffer farthest away from the average is replaced
by the new buffer.
8. Press the Cal Data key to check the function of
the pH electrode. The pH’s of the buffers entered
and the slope of the electrode between every
two points are displayed.
25
Hints to achieve better accuracy:
§ During standardization, allow time for the electrode
to stabilize before entering the buffer into the
meter.
samples
§ Standardize using at least two buffers, bracketing
the expected pH of your samples.
§ Standardize at least daily for the most accurate
readings.
mV
§ Open the fill hole on the electrode.
4
7
10
pH buffers
§ Stir all buffers and samples.
§ Rinse the electrode with deionized water between
samples and buffers.
§ Always use fresh buffers.
Clearing Buffers
Press Standardize, then press 3) Clear buffers
(clear all calibration points) to clear buffers.
If all previously entered buffers will be re-entered,
it is not necessary to clear buffers since the meter
will replace the previous values. If re-entering only
some buffers, all the old buffers should be cleared.
26
mV Mode
Electrode Potential, mV
Redox Titration
Millivolt measurements are used to measure ORP
(oxidation-reduction potential) or redox potential,
to check performance of pH or ion selective
electrodes (see page 45).
The meter will measure millivolts (mV) by pressing
Mode and selecting 2) mV. Relative mV can be
measured by entering a mV offset or using the
current mV value as the mV offset.
Titrant Volume, ml.
Channel A: mV mode
Standardize Menu
1) Auto-enter mV offset
2) Set mV offset
3) Clear mV offset
4) Options menu
mV Mode Standardization Menu
In mV mode, press Standardize and the mV mode
Standardize Menu appears:
1. Auto-enter mV offset: use to set the relative mV
offset equal to the current mV reading. The current
mV becomes 0.0 relative mV.
2. Set mV offset: use to manually enter a mV offset.
3. Clear mV offset: use to clear any offset that has
been entered, returning the meter to absolute mV
mode.
Standardize
Channel A: mV mode
Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set mV offset
6) Standardize
7) Select temperature probe type
4. Options menu: a menu of additional settings
specific to the mV mode. See below.
mV Mode Options Menu
1. Select resolution: use to set mV readings to 1 or
0.1 millivolt resolution.
2. Select stability criteria: use to set the stability
criteria to slow, medium or fast to match the
electrode’s speed and stability of response, providing
tight, medium and loose requirements for a “stable”
( S ) indication.
3. Select signal averaging: use to set the meter to
average readings that are very slow (10 readings),
slow (8), medium (6), fast (4) or very fast (2).
27
4. Set standardization delay: use to set a time for
the meter to wait before entering a relative mV
standardization.
5. Set mV offset: use to manually enter a mV offset
(same as in the mV Standardize menu).
6. Standardize: returns to the mV Standardize Menu.
7. Select temperature probe type:
1) Auto-Detect
20°C…40°C (66°F…104°F, 293 K…313 K)
Auto-Detect will work only if the temperature
is between 20°C and 40°C when the sensor is
connected to the meter.
2) NTC 10 k O
3) NTC 30 k O
4) PT1000
Sartorius uses an NTC 10 k O-sensor as standard
equipment.
Clearing Relative mV Mode
Press Standardize, then press 3) Clear mV offset
to clear offset and return the meter to absolute
mV mode.
28
Quick Start Guide
for Ion Measurements
BNC
1. Connect the Ion Selective Electrode (ISE) and
Reference Electrode, if required, to the meter.
“Combination” ISEs have a reference electrode
built-in, and do not require a separate reference
electrode or connection.
reference electrode
reference
electrode
measuring
or combination ISE
Example:
+
1 mg/L
10 mg/L
Channel A:
Select Mode
1) pH
2) mV
3) Ion
ISA
Ionic Strength
Adjuster
2. Prepare two or more ion standards at concentrations
bracketing typical sample solutions. Add the
appropriate Ionic Strength Adjuster solution to each
standard.
3. Set the meter to display the correct channel
(the channel with the ISE attached, either A or B)
using the Channel key. Set the meter to Ion mode:
press Mode, then 3) Ion.
Note: The meter will display “— — —” (no valid data)
until an ion standard has been entered.
Mode
4. Immerse the electrode(s) in the standard, provide
stirring (a magnetic stirrer is recommended), and
allow sufficient time (1 to 5 minutes depending on
the ISE) for the electrode to reach a stable signal.
Channel
Channel A: ion mode
Standardize Menu
1) Enter a standard
2) Set ion slope
3) Clear standards
4) Options menu
5) Cal reminder menu
5. Press Standardize, 1) Enter a standard, and
follow the prompts to enter an ion name and units.
Custom ion names can be entered or read by selecting Custom in Select Ion Name. To enter additional
ion standards, press Standardize and follow the
prompts displayed. See the Standardizing and
Measuring Ion section for more information.
29
Channel A: ion mode
Ion
Cal Data
1.00 mg/l 08/01/1998 08:32 AM
57.15 mV/decade
10.0 mg/l 08/01/1998 08:35 AM
Cal Data
30
6. Check the ISE response by pressing Cal Data to see
the standards and the ISE slope between calibration
points (standards). The meter will allow an ion
electrode slope between 5.92 mV/decade (10%
slope) and 70.99 mV/decade (120% slope).
Ion Mode
Channel A: ion mode
Standardize Menu
1) Enter a standard
2) Set ion slope
3) Clear standards
4) Options menu
5) Cal reminder menu
Mode
Standardize
Ion Mode Standardize Menu
Select channel A or B. Press Mode and then press
3) Ion for ion mode. Press Standardize and the Ion
Mode Standardize Menu appears.
1. Enter a standard: use to add a new standard or
update (re-enter) an existing standard. Follow the
prompts. With the first standard, you select the ion
name and units. Use Custom to enter an ion name.
2. Set ion slope: for one-point ion calibration, use to
manually enter a slope for the selected ion electrode.
Useful if the ISE has a known, stable slope so that
measurements can be made after entering a single
ion standard.
Caution: Either enter the slope for 25°C or work
without the ATC probe (temperature sensor). All
manually entered slopes are compensated according
to the measuring temperature.
Note: When two or more standards are entered,
the meter uses the actually determined slope(s).
3. Clear standards: use to clear standards for the
electrode standardization selected.
4. Options Menu: use to set various additional parameters to the ion mode. See below.
5. Cal reminder menu: use to set a timer reminding
you to recalibrate. A CAL! icon will appear on the
main screen and an exclamation mark will appear
beside the standards which need to be re-entered.
Channel A: ion mode
Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set ion slope
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Set isopotential point
0) Enable incremental modes
±) Select temperature probe type
Ion Mode Options Menu
1. Select resolution: use to set the readings to 1, 2,
or 3 significant digit resolution.
2. Select stability criteria: use to set the stability
criteria to slow, medium or fast to match the
electrode’s speed and stability of response, providing
tight, medium and loose requirements for a “stable”
( S ) indication.
31
3. Select signal averaging: use to set filtering of the electrode signal to very slow
(10 readings), slow (8), medium (6), fast (4) or very fast (2). Slower settings give more
stable readings, although may require longer times to reach stability.
4. Set standardization delay: use to set a reading delay time for the meter to wait before
accepting an electrode signal during standardization. Programming a standardization
reading delay helps slow-responding electrodes reach equilibrium before the electrode
signal is accepted. Delays of 1 minute for fast ISEs and 5 to 10 minutes for slow ISEs are
appropriate.
5. Set ion slope: use to enter a known ion electrode slope for a one-point standardization.
6. Standardize menu: returns to the ion mode standardize menu.
7. Manual temperature menu: use to set a manual temperature for use in the absence of an
ATC probe or when manually overriding the ATC.
Important note: The EP (European Pharmacopoeia) provides for measurements using
media already
conditioned to a specific temperature (in other words, without using an ATC probe/
automatic temperature compensation). For direct potentiometry according to the EP, use
manual temperature (actual temperature measured).
8. Data alarm menu: use to enter ion limits. If the limits are exceeded, an alarm indication
“!” is displayed.
9. Set isopotential point: use to manually enter an isopotential point. For the determination
of the isopotential point see page 47.
0. Enable incremental modes: use to turn on the known addition/subtraction type ion
methods. See page 35.
±. Select temperature probe type:
1) Auto-Detect
20°C…40°C (66°F…104°F, 293 K…313 K)
Auto-Detect will work only if the temperature is between 20°C and 40°C when the
sensor is connected to the meter.
2) NTC 10 k O
3) NTC 30 k O
4) PT1000
Sartorius uses an NTC 10 k O-sensor as standard equipment.
32
Standardizing
and Measuring Ion
Channel A: ion mode
A
25.0°C
No standards.
08/01/1998
08:40 PM
1. Set the meter to ion mode (use Mode) and turn on
the channel (use Channel) with the Ion Selective
Electrode (either Channel A or B).
The meter displays “— — —”, indicating no valid
measurement, until at least one ion standard has
been entered.
2. Prepare a standard, and add the appropriate Ionic
Strength Adjuster (ISA) solution to the standard.
3. Immerse the electrode(s) in the solution and stir
continuously.
Channel A: ion mode
Standardize Menu
1) Enter a standard
2) Set ion slope
3) Clear standards
4) Options menu
5) Cal reminder menu
4. Press Standardize, select the correct channel if
prompted to do so, and select 1) Enter a standard
to add a standard. If this is the first standard to be
entered, select the ion name and units.
Follow the prompts. Be sure to allow enough time
for the electrode to reach a stable signal.
Note: The default standardization delay for ion
mode is 30 seconds. This can be set by the user.
See Ion Mode Options Menu, page 31.
Standardize
Channel A: ion mode
Ion Checklist
Prepare standard or sample.
Add Ionic Strength Adjuster.
Rinse electrode.
Place electrode in solution.
Stir moderately.
Press Enter to continue.
5. The meter waits for a stable signal and enters the
standard. The entered standard appears in the display (in single-channel mode). Alternatively, if the
signal is not stable, you can press Enter when
the reading stabilizes according to your tolerance
criteria. The meter then enters the standard.
6. Repeat steps 2 through 5 to enter additional
standards. Up to seven standards can be entered.
With more than one standard, the meter performs
a diagnostic check on the electrode.
33
Helpful Hints:
§ Stir constantly.
§ Allow the electrode time to reach a stable reading
before entering the standard into the meter.
mV
ion standards
log [ion]
§ To achieve better accuracy, standardize using at
least two standards, bracketing the expected range
of your samples.
§ Standardize from low to high concentrations.
§ Always use fresh standards.
§ Use standards and samples near the same
temperature.
§ Remember to add Ionic Strength Adjuster to each
standard and sample.
34
Measuring Ion
Using Known Addition Type
(Incremental Ion) Methods
Channel A: ion mode
Enable Incremental Modes
Display ion soft key?
1) Yes
2) No
If incremental modes are
enabled, a ‘+/– ion’ soft key
will appear on the
measurement screen.
Pressing this key will lead
you through the steps to take
one “known (standard) addition”
or “analate (sample) addition”
incremental ion measurement.
Select Measurement Technique
1) Known (standard) addition
2) Analate (sample) addition
Select ‘known addition’ to add
a small volume of known
standard to the sample.
Select ‘analate addition’ to add
a volume of sample to a
volume of known standard.
The meter will automatically
detect subtraction techniques.
–
The meter provides known (“standard”) addition/subtraction and analate (“sample”) addition/subtraction
incremental methods for measuring ion concentrations.
These advanced ion measurement techniques are useful
in overcoming certain problems in ion analysis.
In Known addition/subtraction, a volume of sample
is obtained, Ionic Strength Adjuster is added, and the
ion electrode potential is obtained. Then, a small volume
of standard is added to the sample, and a second
electrode potential is obtained. From the change in
electrode potential, the ion concentration in the sample
can be calculated. Interference from complexation
and other ions can often be overcome by the known
(standard) addition method.
In Analate addition/subtraction, the ion electrode
is placed in a volume of standard and the potential is
obtained. Then, a small volume of sample is added, and
a second electrode potential is obtained. This method
helps overcome problems from widely differing sample
ionic strengths or temperatures.
+
Channel A: Known addition mode
Incremental Ion Measurement
S
58.7 mV
Prepare sample and note volume.
Add Ionic Strength Adjuster.
Rinse electrode.
Place electrode in solution.
Stir moderately.
Enable Incremental Ion Modes
Press Standardize, select the channel (if necessary),
4) Options menu, 0) Enable incremental modes,
then 1) Yes.
This will “turn on” a special soft key in the main
measuring screen which is a direct access soft key to
start a known addition type measurement.
Press Enter to accept.
35
Channel A: Known addition mode
Incremental Ion Measurement
S
95.2 mV
Delta = 36.5 mV
Add aliquot of known standard.
Stir moderately.
For best accuracy, add volume
until Delta > 4.0 mV.
Note total volume added.
Press Enter to accept.
Using a Known Addition Type Incremental
Ion Measurement
Press the incremental method soft key, select
1) Known (standard) addition or 2) Analyte (sample)
addition. Follow the prompts to place the electrode in
the first solution and obtain a reading, add an aliquot
(a known volume) of standard or sample, obtain
a second electrode reading, and enter the sample
volume, standard volume and concentration.
The meter then displays the calculated ion concentration in the original sample. Press Enter to leave the
result screen and return to the measure screen to use
direct reading ion measurements or start another
known addition type measurement (see page 29).
For analyte addition/subtraction:
Add volume of sample as original volume and volume
of standard as added volume.
These volumes will be marked in the printout as
Vx = volume standard and
Vs = volume sample”
For standard addition/subtraction:
Add volume of sample as original volume and volume
of standard as added volume.
These volumes will be marked in the printout as
Vx = volume sample and
Vs = volume standard”
36
Quick Start Guide for Conductivity/Resistivity/
Salinity/TDS Measurements
1. Connect the conductivity/ATC cell to the meter.
2 Prepare one or more conductivity/resistivity standard
solutions at values near typical sample solutions.
Conductivity/ATC cell
Channel C: Conductivity mode
Select Mode
1) Conductivity
2) Practical salinity
3) NaCl salinity
4) Resistivity
5) Total dissolved solids
Channel
3. Set the meter to display channel C using Channel.
Set the meter to the correct mode using Mode
(Conductivity, Practical salinity, NaCl salinity,
Resistivity or Total dissolved solids). Press Enter
to confirm the setting.
4. Place the conductivity cell in the standard, immerse
the cell past the fill vent hole, then lift and allow
the solution to drain out. Immerse and drain at least
three times to fully flush the inner chamber of the
cell. Gently tap the cell to dislodge any air bubbles.
Press Enter to confirm the setting.
Mode
Channel C: Conductivity mode
Standardize Menu
1) Enter a standard
2) Set cell constant
3) Clear standards
4) Options menu
5) Cal reminder menu
Channel C: Conductivity mode
Cal Data
1.0051 /cm
100.1 µS/cm 08/01/98 08:45 AM
1.0092 /cm
1000 µS/cm 08/01/98 08:47 AM
Cal Data
5. Press Standardize, then 1) Enter a standard
and follow the prompts to enter the value of the
standard. Repeat these steps to enter up to five
conductivity/resistivity standards. Each standard
is displayed in the main measuring screen when
in single-channel display.
6. Check the cell performance by pressing Cal Data to
display the standards and the cell constants between
standards.
37
Conductivity/Resistivity/Salinity/
TDS Menus
Channel C: Conductivity Mode
Standardize Menu
1) Enter a standard
2) Set cell constant
3) Clear standards
4) Options menu
5) Cal reminder menu
1.
2.
Channel
Standardize
3.
4.
5.
6.
7.
Conductivity/Resistivity/Salinity/
TDS Standardize Menu
Turn on Channel C using the Channel key.
Press Standardize and the Standardize Menu
is displayed.
Enter a standard: use to enter or re-enter a conductivity standard. Follow the prompts.
Set cell constant: use to manually enter a known
conductivity cell constant for use with no standards.
If the cell constant is known and stable, then this
allows standardizing the cell without using standard
solutions. If a standard is entered, the actual cell
constant is calculated and used.
Clear standards: use to clear all existing standards.
This is useful if new standards are to be entered.
Options menu: accesses additional settings used
with each conductivity-type mode. See below.
Cal reminder menu: use to set a timer reminding
you when to recalibrate. A CAL! icon appears on
the main screen and an exclamation mark appears
beside those standards which need to be re-entered.
[TDS mode only] Calculate solids factor: use this to
allow the meter to calculate a solids factor.*
[TDS mode only] Set solids factor: use to manually
enter a known solids factor * for a particular sample
type. This factor must be within the range of
0.1…2.0 mg/l/µS/cm.
* The “solids factor” is used to correlate the conductivity measurement with the weight-based TDS
measurement for a sample type.
38
Channel C: Conductivity mode
Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set cell constant
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Select display units
0) Set temperature coeff.
±) Select temperature probe type
Channel C: Conductivity mode
Select display units
1) Automatic
2) Fixed µS/cm
3) Fixed mS/cm
Conductivity Mode Options Menu
1. Select resolution: use to set readings to a resolution
of 1 to 4 significant digits.
2. Select stability criteria: sets stability criteria used to
determine when the meter indicates Stable S .
3. Select signal averaging: use to set filtering of the
cell signal to very slow (average 10 readings), slow
(8), medium (6), fast (4) or very fast (2 readings).
Slower settings give more stable readings and are
recommended with conductivity measurements.
4. Set standardization delay: use to set a reading
delay time used by the meter when entering conductivity standards. Programming a reading delay
helps by ensuring sufficient time for the cell signal
to become stable before being entered into the
meter.
5. Set cell constant: use to manually enter a known
conductivity cell constant. (Same as in “Standardize
Menu”).
6. Standardize Menu: use to return to the “Standardize Menu”.
7. Manual temperature menu: use to set a manual
temperature for use in the absence of an ATC probe
or when manually overriding the ATC.
8. Data alarm menu: use to set ion limits to be
entered. If the limits are exceeded, an alarm indication “!” is displayed along with the reading.
9. Select display units: the meter automatically
switches between µS/cm and mS/cm in conductivity,
or between ohm-cm, kiloohm-cm and megohm-cm
in resistivity. If it is better to display a fixed unit, the
“Fixed” settings allow that.
39
Channel C: Conductivity mode
Select Reference Temperature
1) 15°C
2) 20°C
3) 25°C
0. Set temperature correction: use to enable or disable
temperature correction and (when temperature correction is enabled) to set the reference temperature
to correct all conductivity and TDS measurements,
and to set the temperature coefficient for the
temperature correction. Salinity measurements by
definition are corrected to 20°C. Resistivity measurements are not temperature-corrected.
±. Select temperature probe type:
1) Auto-Detect
20°C…40°C (66°F…104°F, 293 K…313 K)
Auto-Detect will work only if the temperature
is between 20°C and 40°C when the sensor is
connected to the meter.
2) NTC 10 k O
3) NTC 30 k O
4) PT1000
Sartorius uses an NTC 10 k O-sensor as standard
equipment.
Channel C: Conductivity mode
Set Temperature Coefficient
Enter the value for this solution:
1.90 %/°C
Press Enter to accept.
Standardizing and Measuring Conductivity/
Resistivity/Salinity/TDS
1. Set the meter to display channel C (use Channel).
Set the meter to the correct mode (Conductivity,
Resistivity, NaCl salinity, Practical salinity or Total
dissolved solids) using Mode.
2. Place the conductivity cell in the standard, immerse
the cell past the fill vent hole, then lift and allow
the solution to drain out. Immerse and drain at least
three times to fully flush the inner chamber of the
cell. For precision measurements, pour off the standard: Immerse the cell in the new (fresh) standard
and gently tap the cell to dislodge any air bubbles.
Channel C: Conductivity mode
Standardize Menu
1) Enter a standard
2) Set cell constant
3) Clear standards
4) Options menu
5) Cal reminder menu
40
3. Press Standardize, select the channel if necessary,
then 1) Enter a standard and follow the prompts to
enter the value of the standard. Repeat these steps
to enter up to five conductivity/resistivity standards.
Each standard is displayed in the main measuring
screen when in the single-channel display.
Use multiple standards that cover the range of values expected in samples. Generally, standards should
be a factor of ten apart in conductivity.
Conductivity Measurements
1. Turn on meter. Allow 1 hour for “warm – up” of
the instrument.
C
µS/cm
M 25.0°C
No standards
R-7
02-08-2006
2. Prepare (or have available) Conductivity Solutions of
known values (preferably traceable to NIST).
09:09:42
Channel C: conductivity mode
Standardize Menu
3. Press the Standardize macro key once. Choose item
“3” for Conductivity.
4. Clear the previous calibration stored in memory by
pressing item # 3 “Clear Calibration”, then pressing
item #1 “Yes” and then “Enter”. This will clear the
previous calibration.
1) Enter a standard
2) Set cell constant
3) Clear standards
4) Options menu
5) Cal reminder menu
Channel C: conductivity mode
Conductivity Standardization
Enter value
of standard:
µS/cm
5. Set the meter for a new calibration (See step 6).
Although a single-point calibration can be used,
greater accuracy can be obtained using two or more
points of calibration. Pick standards that “bracket”
the sample(s) to be analyzed. (i.e. if the sample
is expected to be about 500 _S, use standards of
70 and 1413, or similar values. If the sample conductivity is unknown, use a wider range. An example
would be 70 to 18000 _S.
Press Enter to accept.
Channel C: conductivity mode
Conductivity Standardization
Standardization delay:
00:22
94.1
Press Enter to accept.
O
6. To start the Standardization, press item #1 Enter a
Standard (See picture in step #4). The user will be
prompted to rinse the cell 3 times and refill the cell
with the solution to be analyzed. (Fill the cell
enough to cover the open sensor portion of the
probe), then press “Enter”. A screen will ask for the
calibration value of the solution. Input the value and
press “Enter”. The instrument will display a numeric
value in Ohms of resistance for 30 seconds while the
instruments allows for a stable reading.
41
Channel C: Conductivity mode
Cal Data
Temp Correction: Off
02-08-2006
09:37:14
70.0 µS/cm
(6340 O)
(0.4441 /cm)
Channel C: Conductivity mode
Cal Data
Temp Correction: Off
02-08-2006
09:38:56
18000 µS/cm
(28.6 O)
(0.4438 /cm)
02-08-2006
09:37:14
70.0 µS/cm
(6340 O)
7. (OPTIONAL – Skip to step 9 if opting NOT to see
the first cell constant) After the instrument has
obtained the first calibration point, the cell constant value can be observed by pressing the Cal
Data macro key.
8. To continue calibration and set a second standard,
press the measurement screen key (Second button
down on the right-hand side of the display screen).
9. Repeat step 6 with the second standard, then press
the Cal Data macro key to display the updated cell
constant. (Keep in mind that the cell constant has
been replaced with the new value for both calibration points.)
10. To analyze samples, press the measurement screen
button as described in step 8.
C
s
1800
23.4°C
18000 µS/cm
70.0 µS/cm
02-08-2006
42
µS/cm
R-3
09:44:48
11. Analyze the samples by immersing the probe into
the solution far enough to completely cover the
open portion of the probe sensor area. Allow a few
seconds for a stable reading.
Data Logging
Data Log Menu
1) View data log
2) Turn logging On/Off: ON
3) Set logging interval
4) Clear data log
5) Set sample number
6) Set sample label
7) Print data log
The meter will store up to 620 data points in an
internal data log. Press Print when in the main
measuring screen to store the current result with
channel, stability, temperature, result, units, sample
label, sample number, and date and time in the
data log. Print also outputs this data through the
RS-232 serial port.
All channels displayed are printed and data is logged.
Number of points in log:
350
Data Log
A S 25.3°C
4.176 pH
00102 06/26/98 9:32AM
B S 25.4°C
1.03 mg/L F00101 06/26/98 9:31AM
A S 25.3°C
6.713 pH
00100 06/26/98 9:31AM
Data Log Menu
Press Data Log and the Data Log Menu will appear.
1. View data log: shows the stored data, one screen
at a time. Press the arrow soft keys to page up and
down through the stored data. Press Clear or Enter
to return to the menu.
2. Turn logging on/off: turns the data logging on or
off for all displayed channels.
Set Logging Interval
Enter the time between
data samples.
01 : 00 : 00 hr:min:sec
3. Set logging interval: allows you to enter the time
interval for automatic data logging.
4. Clear data log: clears all stored data points from
memory.
5. Set sample number: allows a number to be assigned
to the first sample. This number will increment for
each consecutive sample logged.
Please Enter to accept
6. Set sample label: A custom user-definable name
can be entered. This label will be printed and stored
with all data. To enter a label, repeatedly press a
number key to select the desired character, then
press Enter. Repeat for all characters, then scroll up
or down to “save and exit” and press Enter.
.
43
Printing Log
7. Print data log: use to send all data points in the
data log to the RS-232 serial port.
Points remaining:
321
Press any key to abort printing.
Note:
Meter settings and electrode standardization data
are kept in the separate non-volatile memory.
Unplugging the meter has no effect on these stored
items.
44
Troubleshooting
Testing the Electrode and Meter
To test the meter for correct operation with a pH,
ORP or ion selective electrode, short the BNC input
connector (either Channel A or B) using the BNC Shorting Cap that was supplied with the meter on the BNC
connector(s). Select the correct channel using Channel.
Select mV mode by pressing Mode and selecting 2) mV.
Verify that the meter is in absolute mV mode (display
shows “mV”, not “rel mV”).
If the meter reads 0 ± 0.3 mV* and is stable, the meter
is measuring correctly.
To test the pH electrode, place it in a fresh pH 7 buffer.
Select the correct channel for the electrode using
Channel. Press Mode and select 2) mV.
Verify that the meter is in absolute mV mode (display
shows “mV”, not “rel mV”) and note the mV reading.
Repeat for either a pH 4 or pH 10 buffer. If the
electrode potential is within the limits shown,
it is measuring correctly.
pH 7 0 ± 30 mV
pH 4 159 to 186 mV higher than pH 7 reading
pH 10 159 to 186 mV lower than pH 7 reading
45
Technical Specifications
Models PP-15, PP-20, PP-25 and PP-50
Modes
Range
Resolution
Accuracy
Temperature compensation
Slope control
Ambient conditions
Power requirements
Equipment supplied
Meter kit with electrode kit
includes:
Meter only kit includes:
pH
mV
Temperature
–2.000 to 20.000
±2000.0
–5.0 to 105.0°C
0.001/0.01/0.1
0.1/1
0.1
±0.002
±0.1
± 0.3
Automatic & manual: –5 to 105°C
Automatic, 90 to 105% and manual, 80–120%
15 to 40°C, humidity from 0 to 90% (non-condensing)
230 V 50/60 Hz (Additional voltages available)
Meter, power supply, high performance glass-body pH/ATC
“3-in-1” electrode, electrode arm, AC adapter and operation manual.
Meter, AC adapter and operation manual.
Models PP-25 and PP-50
Mode
Range
Resolution
Accuracy
Slope control
Number of standards
Ion
1.00E 9 to 9.99E 9
1, 2, or 3 significant figures
± 0.17%n (n = ion charge)
Automatic or manual, 5.92 mV/decade (10% slope)
to 70.99 mV/decade (120%)
7
Models PP-20 and PP-50
Mode
Range*
Resolution
Accuracy
Input resistance
Cell constant
Temperature coeff.
Number of standards
Practical
Conductivity
Resistivity
Salinity
0.5–
50 O · cm –
0.01 –
20,000 µS/cm 2 M O · cm
42 ppt
1, 2, 3, or 4 significant figures
± 0.5%
10 · 1012O
Automatic or manual, 0.01–100
Off or On (0 – 4%/°C)
5
* Specifications are based on a cell constant of 1”
46
NaCl
Salinity
0.01 –
70 ppt
TDS
0.005 –
300,000
pH Theory
More Basic
More Acidic
pH
0
1
2
3
4
5
6
Neutral 7
8
9
10
11
12
13
14
Strong Acid
The measurement of pH plays an important role in
water quality applications as well as in industry and
research. pH is a measure of acidity or alkalinity of a
solution, and is usually written:
Lemon Juice
Tomato Juice
Coffee
Pure water
Baking Soda
Ammonia
Strong Base
pH scale showing the relative
acidity or basicity of some common
substances
pH = -log [H3O+]
where [H3O+] is the concentration of oxonium ions.
pH levels generally range from 0 to 14, with a pH value
of 7 being the neutral point. pH values greater than 7
are alkaline, and pH values less than 7 indicate acidic
solutions.
Conventional pH meters use a combination glass pH
electrode, which includes a reference electrode.
The reference electrode provides a stable reference
point and completes the electrical circuit.
The pH meter reads the voltage of the two electrodes,
converts it to pH units, and displays the result.
The electrode signal varies with the pH, according to
the Nernst equation:
E = E0 + S · log [H3O+]
Where:
E
= measured electrode potential
E0
= standard potential of the system (constant)
S
= slope
47
Ion Selective Electrode Theory
The measurement of ions plays an important role in
water quality applications, industry, research and environmental monitoring. Ion-selective Electrodes (ISEs)
respond, more or less exclusively, to a specific type of
ion in solution. The particular ion to which an ISE
responds depends on the chemical makeup of its sensing membrane. ISEs operate according to a form of the
Nernst equation:
E = E0 + S · log [ion]
48
Determining the Isopotential Point
The isopotential point is the potential of an electrode system which does not change with
temperature. The slope of an electrode changes with temperature according to the Nernst
equation. At temperatures <25°C, the Nernst factor is <59.16 mV; at temperatures >25°C,
the Nernst factor is >59.16 mV. (This is automatically allowed for with automatic temperature compensation.) In addition, the zero point of an electrode may change depending
on the temperature. For accurate pH measurements, this change should be allowed for.
This is done by determining the isopotential point of the pH or ion selective electrode for
several buffers at various temperatures. Typical pH electrodes have isopotential points
near zero mV (which is the default setting for the meter). For high accuracy pH measurements, or for ion-selective measurements where the sample temperature may widely vary,
the isopotential point of the pH or ion-selective electrode may be experimentally determined and entered into the meter.
§ Prepare a set of buffers or ion standards bracketing the linear range of the electrode.
Place the buffers or standards in a temperature bath at known temperature. Then place
them in a bath at a 2nd temperature.
§ Place the meter into mV mode.
§ Measure and record mV readings of each pH or concentration, and repeat at several
temperatures.
§ Plot the log of concentration or pH value versus mV reading.
§ Connect the points for each temperature.
§ To enter the isopotential point, access the pH mode, press Standardize; then select
“4. Options Menu” and afterwards, “9. Set isopotential point.”
! Where the lines intersect is the isopotential point.
pH Electrode Isopotential Point
Ion Electrode Isopotential Point
–180
200
25°C
150
10°C
Electrode
Output 100
(V/mV)
Isopotential Point:
3.07 + 10–4 mol/l, 162 mV
Electrode
Output
(V/mV)
10°C
0
Isopotential Point:
pH = 7.04 at V = 9 mV
50
60°C
180
0
10–3
Log of Ion Concentration, moles/L
10–4
4
7
10
pH
49
RS-232 Serial Interface Meter Command Set
Sartorius Professional Meters have a bidirectional RS-232 serial port, which can be used to
send commands to the meter and output data from the meter. Special characters (W, µ, é)
are coded using ASCII (not ANSI); use an ASCII font like “Terminal”. Also use a terminal
emulation like TTY or ANSI, not VT100.
Serial commands follow either “keystroke” mode or high level command mode consisting
of “SET”, “GET” and “DO” instructions.
(Note: “GET” and “DO” are optional).
Keystroke instructions
KEYS
M
mode
Z
standardize
C
cal data
H
channel
S
setup
L
data log
R
clear
N
enter/print
[0 to 9] equivalent to pressing a numeric key
–
+/– key
.
decimal key
E
used within a number to enter values in exponential form
!
press soft key #1 (at top, usually help)
@
press soft key #2 (usually return to measuring screen)
#
press soft key #3 (usually up arrow)
$
press soft key #4 (at bottom, usually down arrow)
Notes:
Key commands are acknowledged by the meter with a reply.
Keys = COMMAND_RECEIVED. Multiple keys can be concatenated together into a single
command, for example, keys Z413@ (Standardize, options, resolution, set to 3, main),
or keys Z4721.2-N@ (Set manual temperature to -1.2).
50
High Level Instructions
Use commands SET, GET, DO.
Follow command by a keyword like MODE, STDZPH, STDZCONDO, CALDATA, STDZCLEAR,
CHANNEL, DATETIME, TIMESTAMP, DISPLAY, READ, INFO
Typical Syntax: [command] [keyword] [channel] [variable(s)]
Error conditions are replied to with an Error response; for example:
“Error: Need channel”, “Error: Need mode”, “Error: Unspecified”.
Accepted commands have a response; indicated below for each command.
Mode Operations
set mode “channel character” “mode id”
Examples: SET MODE A PH
SET MODE B MV
SET MODE B ION
SET MODE C CONDUCTIVITY
Returns confirmation; for example “SET MODE A PH” returns “A mode = PH”. Valid modes
are (depending on the meter model) PH, MV, CONDUCTIVITY, RESISTIVITY, PRAC_SALINITY,
NACL_SALINITY, DISSOLVED_SOLIDS.
[get] mode “channel character”
Examples: [GET] MODE A
Returns mode information on selected channel; “A Mode = MV”.
Channel operations
set channel “channel character” “on|off”
Examples: SET CHANNEL A ON
SET CHANNEL C OFF
Returns confirmation; for example “A Channel = ON”.
[get] channel “channel character”
Example:
[GET] CHANNEL B
Returns channel information; “A Channel = OFF”.
(DO) READ “channel character” (Take reading with temperature without sending to data log).
51
Standardization operations
[DO] STDZPH “channel character”
[DO] STDZCLEAR “channel character”
[DO] STDZCONDO “conductivity standard value”
[DO] CALDATA “channel character”
(DO] STDZPH A
[Auto-enter a buffer]
Returns “Stdz pH = COMMAND_RECEIVED”, followed by the Calibration Data printout.
[DO] STDZCLEAR A
[Clear all buffers/standards.]
Returns “Stdz Clear = COMMAND_RECEIVED”.
[DO] STDZCONDO 1000
[Enter a standard of 1000 µS/cm.]
Returns “C Stdz Condo = COMMAND_RECEIVED”, followed by the Calibration Data printout.
[DO] CALDATA A
Returns “A Cal Data = COMMAND RECEIVED” followed by the Calibration Data printout.
General Meter Setup Operations
SET DATETIME MM/DD/YYYY HH:MM:SS
[GET] DATETIME
[GET] INFO
(DO) DISPLAY display_text_string (at 0, 0)
(DO) DISPXY x y display_text_string
[Leading 0s required, 24-hour time]
Returns: “MM/DD/YYYY HH:MM:SS”
Returns Model, Version, Serial#.
[x = pixel from left, 0 – 319]
[y = pixel from top, 0 – 239]
Example: DO DISPXY 15 0
Device ready, press any key.
SET TIMESTAMP #
(Set date/time using ’unix’ seconds).
(GET) TIMESTAMP
52
Pin Assignment
Here are the functions for each of the pins:
1
5
6
9
Pin 1:
Pin 2:
Pin 3:
Pin 4:
Pin 5:
Pin 6:
Pin 7:
Pin 8:
Pin 9:
Not connected
TxD
RxD
Not connected
GND
Not connected
Clear to Send (CTS)
Ready to Send (RTS)
Not connected
A 9-pin, “D”-type connector (digital I/O) provides a standard DCE configuration for
a serial RS-232 output.
Wiring Diagram
For connecting a computer or peripheral device to the pH meter using the RS-232C/V24
port for interface cables up to 15 m.
No other pins on the pH meter may be connected.
pH meter, 9-pin
Computer/Printer, 9-pin
T+D 2
2
R+D 3
3
CTS 7
4
RTS 8
8
GND 5
5
Most Common Errors
§ The baud rates between the meter and the computer are different
§ The parity or stop bits are not set in the computer properly
§ The pins in the cable are not properly configured
Software
The meter transmits and receives ASCII strings.
53
Maintenance
This product contains no user-serviceable parts.
All replacement parts should be obtained from Sartorius
or an authorized distributor.
Cleaning
The exterior surfaces of this product may be cleaned
with a damp cloth or with mild detergent.
!
54
Caution
Changes or modifications not expressly approved by
the manufacturer will void the user’s warranty for this
equipment.
Menu Tree Diagram
Mode (A, B)
1) pH
2) mV
3) Ion
pH Standardize Menu
1) Auto-enter a buffer
2) Manual buffer entry
3) Clear buffers
4) Options menu
5) Cal reminder menu
6) Select buffer set
pH Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set pH slope
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Set isopotential point
0) Select temperature probe type
mV Standardize Menu
1) Auto-enter mV offset
2) Set mV offset
3) Clear mV offset
4) Options menu
mV-Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set mV offset
6) Standardize menu
7) Select temperature probe type
Ion Standardization Menu
1) Enter a standard
2) Set ion slope
3) Clear standards
4) Options menu
5) Cal reminder menu
Ion Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set ion slope
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Set isopotential point
0) Enable incremental modes
±) Select temperature probe type
Conductivity Mode (C)
1) Conductivity
2) Practical salinity
3) NaCl salinity
4) Resistivity
5) Total dissolved solids
Conductivity Standardize Menu
1) Enter a standard
2) Set cell constant
3) Clear standards
4) Options menu
5) Cal reminder menu
6) Calculate solids factor
(TDS mode only)
7) Set solids factor
(TDS mode only)
55
Conductivity Options Menu
1) Select resolution
2) Select stability criteria
3) Select signal averaging
4) Set standardization delay
5) Set cell constant
6) Standardize menu
7) Manual temperature menu
8) Data alarm menu
9) Select display units
0) Select temperature correction
±) Select temperature probe type
Setup Menu
1) Time and date menu
2) Select temperature units
3) Select contrast
4) Language
5) Setup serial port
6) Keypress beep on/off
7) Select display background
8) Show meter information
9) Enable measure lock
0) Enable strict calibration
±) Set screen saver timeout
.) Restore factory defaults
56
Data Log Menu
1) View data log
2) Turn logging On/Off: OFF
3) Set logging interval
4) Clear data log
5) Set sample number
6) Set sample label
7) Print data log
Accessories
Order No.
pH/ATC combination Electrodes:
Plastic body with built-in temperature sensor, KCI filling
Glass body with integrated temperature sensor, KCl filling
platinum junction
Plastic body with integrated temperature sensor; gel-filled
Plastic body, gel-filled, fiber junction
Glass body, KCI filling, platinum junction
ATC probe
Data printer
Paper (5 rolls, each 50 m)
Ribbon
pH buffers
supplied in packages of 50 capsules. The contents of each capsule
are dissolved in 100 ml of distilled water
pH = 4.01 ± 0.02 at 25°C
pH = 7.00 ± 0.02 at 25°C
pH = 9.00 ± 0.02 at 25°C
pH = 10.00 ± 0.02 at 25°C
Color-coded buffer solution in a twin-neck bottle;
eliminates the need for using a beaker during calibration
(standardization; traceable to NIST Standards)
pH = 4.00 ± 0.01 at 25°C, 500 ml
pH = 7.00 ± 0.01 at 25°C, 500 ml
pH = 10.00 ± 0.01 at 25°C, 500 ml
Storage solution, for pH electrodes, 500 ml
Cleaning solution, pepsin/hydrochloric acid, 500 ml
Electrolyte solution, KCI (3 moles/L), free of silver ions, 500 ml
Conductivity/resistivity standards, traceable to NIST Standards
0.084 mS/cm ± 1.0 % at 25°C, 500 ml
0.147 mS/cm ± 1.0 % at 25°C, 500 ml
1.413 mS/cm ± 1.0 % at 25°C, 500 ml
12.88 mS/cm ± 1.0 % at 25°C, 500 ml
PY-P10
PY-P11
PY-P12
PY-P20
PY-P21
PY-T01
YDP05-PH
6906937
6906918
PY-Y01
PY-Y02
PY-Y03
PY-Y04
PY-Y21
PY-Y22
PY-Y23
PY-Y05
PY-Y06
PY-Y07
PY-Y10
PY-Y11
PY-Y12
PY-Y13
Additional pH/ATC electrodes and connectors for special measuring conditions as well as ion
selective electrodes or redox (ORP) electrodes are available upon request.
57
C Marking
The C marking affixed to the equipment indicates that the equipment meets the requirements of the following Directive(s):
Council Directive 89/336/EEC ”Electromagnetic compatibility (EMC)”
1.1 Source of 89/336/EEC: Official Journal of the European Communities,
No. 2001/C 105/03
EN 61326
Part 1:
Electrical equipment for measurement, control and laboratory use
EMC requirements
General requirements
Limitation of emissions: Industrial areas, Class A
Defined immunity to interference: Minimun requirements,
non-continuous operation
Warning!
This is a Class A device that can cause radio interference in residential areas.
If this should occur, the user may be required to take suitable measures to correct and
eliminate such interference.
Under extreme electromagnetic influences, e.g., while operating radio equipment in the
direct vincinity of the Professional Meter, this may affect the meter’s readings. Once this
radio interference has subsided, the product (Professional Meter) will operate according
to its specifications.
Important Note:
The operator shall be responsible for any modifications to Sartorius equipment and for
any connections of cables or equipment not supplied by Sartorius and must check and,
if necessary, correct these modifications and connections. On request, Sartorius will provide
information on the minimum operating specifications (in accordance with the Standards listed above for defined immunity to interference).
Council Directive 73/23/EEC
”Electrical equipment designed for use within certain voltage limits”
Applicable European Standards:
EN 60950
Safety of information technology equipment including
electrical business equipment
EN 61010
Safety requirements for electrical equipment for measurement,
control and laboratory use
Part 1: General requirements
If you use electrical equipment in installations and under ambient conditions requiring higher safety standards, you must comply with the provisions as specified in the applicable regulations for installation in your country.
58
Sartorius AG
Weender Landstrasse 94–108
37075 Goettingen, Germany
Phone +49.551.308.0
Fax +49.551.308.3289
www.sartorius.com
Copyright by Sartorius AG,
Goettingen, Germany.
All rights reserved. No part
of this publication may
be reprinted or translated in
any form or by any means
without the prior written
permission of Sartorius AG.
The status of the information,
specifications and illustrations
in this manual is indicated
by the date given below.
Sartorius AG reserves the
right to make changes to the
technology, features,
specifications and design of the
equipment without notice.
Status:
October 2006, Sartorius AG,
Goettingen, Germany
Printed in Germany on paper that has
been bleached without any use of chlorine
W1A000 · KT
Publication No.: WPP6003-e06104
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