AKTA start Operating Instructions

AKTA start Operating Instructions
ÄKTA™ start
Operating Instructions
Original instructions
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Table of Contents
Table of Contents
1
Introduction ..........................................................................................................
1.1
1.2
1.3
1.4
2
3
8
9
11
14
Safety instructions ...............................................................................................
17
2.1
2.2
2.3
2.4
2.5
Safety precautions ...............................................................................................................................
Labels .........................................................................................................................................................
Emergency procedures ......................................................................................................................
Recycling procedures .........................................................................................................................
Declaration of Hazardous Substances (DoHS) ........................................................................
18
26
30
32
33
System description ..............................................................................................
36
3.1
3.2
3.3
37
41
44
System overview ...................................................................................................................................
Instrument ...............................................................................................................................................
Instrument Display ...............................................................................................................................
3.3.1
3.3.2
3.3.3
3.3.4
4
5
7
About this manual ................................................................................................................................
Important user information .............................................................................................................
Regulatory information ......................................................................................................................
Associated documentation ..............................................................................................................
Overview of the Instrument Display ......................................................................................
Description of Method run .........................................................................................................
Description of Create method ..................................................................................................
Description of Settings and service ........................................................................................
45
50
53
54
Installation ............................................................................................................
62
4.1
4.2
4.3
4.4
4.5
Space requirements ............................................................................................................................
Transport ÄKTA start and Frac30 ..................................................................................................
Unpack ÄKTA start and Frac30 ......................................................................................................
Accessories package ..........................................................................................................................
Install ÄKTA start ...................................................................................................................................
63
65
67
77
78
Prepare the system for a run .............................................................................
86
5.1
5.2
5.3
87
90
91
Flow path overview .............................................................................................................................
Start the instrument ............................................................................................................................
Calibrations .............................................................................................................................................
5.3.1
5.3.2
5.3.3
5.3.4
5.3.5
5.3.6
5.4
Calibration guide ............................................................................................................................
Instrument Display calibration .................................................................................................
Pressure sensor zero offset ........................................................................................................
Pump calibration ............................................................................................................................
UV Monitor calibration .................................................................................................................
Conductivity Monitor calibration .............................................................................................
92
93
95
97
99
103
System performance ..........................................................................................................................
109
5.4.1
5.4.2
5.4.3
5.4.4
5.5
System performance method ...................................................................................................
System performance method from ÄKTA start .................................................................
System performance method from UNICORN start .......................................................
Switch valve timing .......................................................................................................................
110
112
117
120
Connect a column ................................................................................................................................
124
ÄKTA start Operating Instructions 29-0270-57 AF
3
Table of Contents
5.6
Run Prepare system methods ........................................................................................................
5.6.1
5.6.2
5.6.3
5.6.4
129
131
133
136
Sample application ..............................................................................................................................
Prepare the Fraction collector ........................................................................................................
Cold room operations .........................................................................................................................
Starting a run ..........................................................................................................................................
138
150
154
156
Operations from the Instrument Display ........................................................
158
6.1
6.2
6.3
159
162
165
5.7
5.8
5.9
5.10
6
Introduction .............................................................................................................................................
Fractionation ..........................................................................................................................................
Perform a manual run ........................................................................................................................
6.3.1
6.3.2
6.4
Manual run .......................................................................................................................................
Monitor and control the run ......................................................................................................
166
168
Perform a method run ........................................................................................................................
173
6.4.1
6.4.2
6.4.3
6.4.4
6.4.5
6.5
6.6
Select a method type ....................................................................................................................
Quick start .........................................................................................................................................
Templates ..........................................................................................................................................
User defined methods ..................................................................................................................
Prepare system methods ............................................................................................................
174
176
182
190
192
Procedures after a run .......................................................................................................................
Manage methods and files ..............................................................................................................
195
198
6.6.1
6.6.2
6.6.3
7
8
Create method ................................................................................................................................
Handling the USB memory stick ..............................................................................................
BMP result file ..................................................................................................................................
199
208
211
Operations from UNICORN start .......................................................................
213
7.1
7.2
7.3
7.4
System Control .....................................................................................................................................
Method Editor .........................................................................................................................................
Evaluation ................................................................................................................................................
Administration ........................................................................................................................................
214
216
217
218
Maintenance .........................................................................................................
219
8.1
8.2
8.3
220
223
224
Regular maintenance .........................................................................................................................
Cleaning before planned maintenance/service .....................................................................
Cleaning the system flow path .......................................................................................................
8.3.1
8.3.2
8.4
8.5
8.6
Disconnect the column ................................................................................................................
System cleaning .............................................................................................................................
225
226
Cleaning the UV flow cell ..................................................................................................................
Cleaning the Conductivity flow cell ..............................................................................................
Other cleaning procedures ..............................................................................................................
229
231
232
8.6.1
8.6.2
8.6.3
8.7
4
128
Pump wash A ...................................................................................................................................
Pump wash B ...................................................................................................................................
Washout fractionation tubing ..................................................................................................
Column preparation ......................................................................................................................
Cleaning the inlet filters ..............................................................................................................
Cleaning the instrument externally ........................................................................................
Cleaning the Fraction collector ................................................................................................
233
234
235
Storage of the instrument ................................................................................................................
238
ÄKTA start Operating Instructions 29-0270-57 AF
Table of Contents
8.8
Replacement of tubing and filters ................................................................................................
8.8.1
8.8.2
240
Replace the inlet filters ................................................................................................................
Replace the tubing and connectors .......................................................................................
241
242
Troubleshooting ...................................................................................................
244
9.1
9.2
9.3
Introduction to troubleshooting .....................................................................................................
Basic troubleshooting .........................................................................................................................
System error report .............................................................................................................................
245
247
258
10 Reference information ........................................................................................
260
9
10.1
10.2
10.3
10.4
10.5
Specifications .........................................................................................................................................
Chemical resistance ............................................................................................................................
Literature ..................................................................................................................................................
Health and Safety Declaration Form ...........................................................................................
Ordering information ..........................................................................................................................
261
267
270
271
273
11 Appendix ................................................................................................................
277
11.1
System Performance Report ...........................................................................................................
278
Index .......................................................................................................................
281
ÄKTA start Operating Instructions 29-0270-57 AF
5
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1 Introduction
1
Introduction
About this chapter
This chapter contains important user information, description of safety notices, regulatory information, intended use of ÄKTA start, and list of associated documentation.
In this chapter
Section
See page
1.1 About this manual
8
1.2 Important user information
9
1.3 Regulatory information
11
1.4 Associated documentation
14
ÄKTA start Operating Instructions 29-0270-57 AF
7
1 Introduction
1.1 About this manual
1.1
About this manual
Purpose of this document
The Operating Instructions provide you with the instructions needed to install, operate
and maintain ÄKTA start in a safe way.
Nomenclature conventions
The nomenclature used in this manual are explained in the table below.
Concept
Explanation
ÄKTA start
The instrument.
Frac30
The Fraction collector.
UNICORN™ start
The software installed on a computer.
ÄKTA start System
The entire liquid chromatography system, including instrument,
Fraction collector and software.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Buffer valve).
8
ÄKTA start Operating Instructions 29-0270-57 AF
1 Introduction
1.2 Important user information
1.2
Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, operating or
maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use
ÄKTA start is a liquid chromatography system used for preparative purification of proteins
at laboratory-scale. The system can be used for a variety of research purposes to fulfill
the needs of the users in the academia and in the life sciences industry.
ÄKTA start is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.
Prerequisites
In order to follow this manual and use the system in the manner it is intended, it is important that:
•
you understand the concepts of liquid chromatography
•
you have read and understood the Safety instructions chapter in the ÄKTA start
Operating Instructions.
ÄKTA start Operating Instructions 29-0270-57 AF
9
1 Introduction
1.2 Important user information
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
10
Note:
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip:
A tip contains useful information that can improve or optimize your procedures.
ÄKTA start Operating Instructions 29-0270-57 AF
1 Introduction
1.3 Regulatory information
1.3
Regulatory information
Introduction
This section describes the directives and standards that are fulfilled by ÄKTA start.
Manufacturing information
The table below summarizes the required manufacturing information. For further information, see the EC Declaration of Conformity (DoC) document.
Requirement
Content
Name and address of manufacturer
GE Healthcare Bio-Sciences AB,
Björkgatan 30, SE 751 84 Uppsala,
Sweden
Conformity with EU Directives
This product complies with the European directives listed in the table below, by fulfilling
the corresponding harmonized standards. For further information, see the EC Declaration
of Conformity document.
Directive
Title
2006/42/EC
Machinery Directive (MD)
2004/108/EC
Electromagnetic Compatibility (EMC) Directive
2006/95/EC
Low Voltage Directive (LVD)
ÄKTA start Operating Instructions 29-0270-57 AF
11
1 Introduction
1.3 Regulatory information
CE marking
The CE marking and the corresponding EC Declaration of Conformity is valid for the instrument when it is:
•
used as a stand-alone unit, or
•
connected to other products recommended or described in the user documentation,
and
•
used in the same state as it was delivered from GE, except for alterations described
in the user documentation.
International standards
This product fulfills the requirements of the following standards:
Standard
Description
Notes
EN 61010-1, IEC
61010-1, UL 61010-1,
CAN/CSA C22.2 No.
61010-1
Safety requirements for
electrical equipment for measurement, control, and laboratory use.
EN standard is
harmonized with EU
directive 2006/95/EC
EN 61326-1, IEC
61326-1
Electrical equipment for
measurement, control and
laboratory use - EMC requirements
EN standard is
harmonized with EU
directive 2004/108/EC
Safety of machinery. General
principles for design. Risk
assessment and risk reduction.
EN ISO standard is
harmonized with EU
directive 2006/42/EC
(Emission according to
CISPR 11, Group 1,
class A)
EN ISO 12100
WARNING
This is a Class A product. In a domestic environment, this product
may cause radio interference, in which case the user may be required to take adequate measures.
12
ÄKTA start Operating Instructions 29-0270-57 AF
1 Introduction
1.3 Regulatory information
FCC statement
ÄKTA start complies with FCC 47 CFR Part 15b.
Note:
This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC Rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment generates,
uses and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be required to
correct the interference at his own expense.
Regulatory compliance of
connected equipment
Any equipment connected to ÄKTA start should meet the safety requirements of EN
61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment
must be CE marked.
Environmental Conformity
Requirement
Description
2011/65/EU
Restriction of Hazardous Substances (RoHS) Directive
2012/19/EU
Waste Electrical and Electronic Equipment (WEEE) Directive
ACPEIP
Administration on the Control of Pollution Caused by Electronic Information Products, China Restriction of Hazardous Substances
(RoHS)
ÄKTA start Operating Instructions 29-0270-57 AF
13
1 Introduction
1.4 Associated documentation
1.4
Associated documentation
Introduction
This section describes the user documentation that is delivered with the instrument and
how to find related literature that can be downloaded or ordered from GE.
User documentation on the CD
The user documentation listed in the table below is available on the ÄKTA start User
Documentation CD. A display help is available from the Instrument Display.
14
Documentation
Main contents
ÄKTA start Operating Instructions
Instructions needed to install, operate
and maintain ÄKTA start in a safe way.
ÄKTA start Instrument Display Help
Dialog descriptions of the functionality
menu for ÄKTA start (only accessible from
the Instrument Display).
ÄKTA start Maintenance Manual
Detailed instrument and module descriptions, including instructions needed to
maintain and troubleshoot ÄKTA start.
ÄKTA start System Cue Card
A condensed guide to prepare and run
chromatographic techniques on ÄKTA
start.
ÄKTA start Maintenance Cue Card
A condensed guide to handling routine
maintenance operations and troubleshooting ÄKTA start.
ÄKTA start Operating Instructions 29-0270-57 AF
1 Introduction
1.4 Associated documentation
From the Help menu in UNICORN start or on the UNICORN start DVD, the following user
documentation is available.
Documentation
Main contents
UNICORN start 1.0 User Manual
Overview and detailed descriptions of the
system control software designed for
ÄKTA start, which includes process picture map for real time monitoring,
method editor, evaluation and administration modules.
UNICORN start Online Help
Dialog descriptions for UNICORN start
(only accessible from the Help menu).
Data files, application notes and
user documentation on the web
To order or download data files, application notes or user documentation, see the instruction below.
Step
Action
1
Go to www.gelifesciences.com/AKTA.
2
Click ÄKTA Lab-Scale Systems.
3
Select ÄKTA start from the list.
4
Click Related Documents.
5
Select to download the chosen literature.
ÄKTA start Operating Instructions 29-0270-57 AF
15
1 Introduction
1.4 Associated documentation
Access documentation from
mobile units
Scan the code using your mobile phone or tablet computer to access the product page
for ÄKTA start. Select documents to download under the Related Documents tab.
16
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2
Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for the
product. The labels on the system and recycling procedures are also described.
In this chapter
This chapter contains the following sections:
Section
See page
2.1 Safety precautions
18
2.2 Labels
26
2.3 Emergency procedures
30
2.4 Recycling procedures
32
2.5 Declaration of Hazardous Substances (DoHS)
33
ÄKTA start Operating Instructions 29-0270-57 AF
17
2 Safety instructions
2.1 Safety precautions
2.1
Safety precautions
Introduction
ÄKTA start is powered by mains voltage and handles liquids that may be hazardous.
Before installing, operating or maintaining the system, you must be aware of the hazards
described in this manual. Follow the instructions provided to avoid personal injuries
or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•
General precautions
•
Using flammable liquids
•
Personal protection
•
Installing and moving the instrument
•
System operation
•
Maintenance
General precautions
WARNING
Always follow these General precautions to avoid injury when using
ÄKTA start.
18
•
Do not operate ÄKTA start in any other way than described in
the ÄKTA start user documentation.
•
Operation and user maintenance of ÄKTA start should be performed according to the instructions described in ÄKTA start
Operating Instructions and ÄKTA start Maintenance Manual.
•
Do not use any accessories not supplied or recommended by
GE.
•
Do not use ÄKTA start if it is not working properly, or if it has
suffered any damage, for example:
-
damage to the power cord, its plug or the Frac30 cable
-
damage caused by dropping the equipment
-
damage caused by splashing liquid onto it
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.1 Safety precautions
Using flammable liquids
WARNING
When using flammable liquids with ÄKTA start, follow these precautions to avoid any risk of fire or explosion.
•
Fire Hazard. Before starting the system, make sure that there
is no unintentional leakage in the instrument or tubing.
•
Explosion hazard. To avoid building up an explosive atmosphere when using flammable liquids, make sure that the room
ventilation meets the local requirements.
CAUTION
To avoid hazardous situations when unpacking, installing or moving
ÄKTA start, follow the precautions below.
ÄKTA start is filled with denaturated alcohol (50% C2H5OH (ethanol))
at delivery. The denatured alcohol mixture can be hazardous to
humans if consumed. Flush out the denaturated alcohol before
assembling, testing or integrating ÄKTA start into the intended
process context.
Personal protection
WARNING
To avoid hazardous situations when working with ÄKTA start, take
the following measures for personal protection.
Spread of biological agents. The operator has to take all necessary
actions to avoid spreading hazardous biological agents in the
vicinity of the equipment. The facility should comply with the national code of practice for biosafety.
ÄKTA start Operating Instructions 29-0270-57 AF
19
2 Safety instructions
2.1 Safety precautions
CAUTION
To avoid hazardous situations when working with ÄKTA start, take
the following measures for personal protection.
20
•
Always use appropriate personal protective equipment during
operation and maintenance of ÄKTA start.
•
Spillage hazard. When using ÄKTA start use personal protective
equipment like goggles, lab coat, protective shoes and gloves
to avoid any circumstances of spillage.
•
Cut injuries. The tubing cutter is very sharp and must be handled with care to avoid injuries.
•
Hazardous substances. When using hazardous chemical and
biological agents, take all suitable protective measures, such
as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe
operation, maintenance and decommissioning of the equipment.
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.1 Safety precautions
Installing and moving the
instrument
WARNING
To avoid damage to person when installing or moving ÄKTA start,
follow the instructions below.
•
Moving the instrument horizontally. One person is recommended when moving the instrument horizontally.
•
Supply voltage. Make sure that the supply voltage at the wall
outlet corresponds to the marking on the instrument, before
connecting the power cord.
•
Power cord. Only use grounded power cords delivered or approved by GE.
•
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power
switch must always be easy to access. The power cord with
plug must always be easy to disconnect.
•
Installing the computer (optional). The computer should be
installed and used according to the instructions provided by
the manufacturer of the computer.
•
Disconnect power. Always switch off power to ÄKTA start before an instrument module is removed or installed, or a cable
is connected or disconnected.
CAUTION
To avoid damage to person when installing or moving ÄKTA start,
follow the instructions below.
Protective ground. ÄKTA start must always be connected to a
grounded power outlet.
ÄKTA start Operating Instructions 29-0270-57 AF
21
2 Safety instructions
2.1 Safety precautions
NOTICE
To avoid damage to ÄKTA start or other equipment when installing
or moving the instrument, follow the instructions below.
•
Vents on ÄKTA start. To ensure adequate ventilation, keep
papers and other objects away from the vents of the instrument.
•
Any computer used with the equipment shall comply with IEC
60950 and be installed and used according to the manufacturer's instructions.
•
Frac30 should not be connected or disconnected from ÄKTA
start when the instrument is powered ON.
System operation
WARNING
To avoid personal injury when operating ÄKTA start, follow the instructions below.
22
•
Rotating the instrument. Make sure that there is always at
least 20 cm of free space around ÄKTA start to allow for sufficient ventilation. When turning or moving the instrument, take
care not to stretch or squeeze tubing or cables. A disconnected
cable may cause power interruption or network interruption.
Stretched tubing may cause bottles to fall, resulting in liquid
spillage and shattered glass. Squeezed tubing may cause increase in pressure, or block liquid flow. To avoid the risk of
knocking over bottles, always place bottles on the buffer tray
and turn or move carefully.
•
Hazardous chemicals during run. When using hazardous
chemicals, run the System cleaning template to clean and flush
the entire system tubing with distilled water, before service and
maintenance.
•
Setting. Check that the correct outlet size settings are used.
Make sure that tubing and fittings are properly connected and
secured. Make sure that the pressure limit settings are correct
before starting the run.
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.1 Safety precautions
CAUTION
To avoid personal injury when operating ÄKTA start, follow the instructions below.
•
Max weight on Buffer tray. Do not place containers with a
volume of more than 1 liter each on the Buffer tray. The maximum allowed weight on the Buffer tray is 5 kg.
•
Large spillage. Switch off ÄKTA start and unplug the power
cord, if large spillage occurs.
NOTICE
To avoid damage to ÄKTA start or other equipment when operating
the instrument, follow the instructions below.
•
Keep UV flow cell clean. Do not allow solutions containing
dissolved salts, proteins or other solid solutes to dry out in the
flow cell. Do not allow particles to enter the flow cell, as damage
to the flow cell may occur.
•
Pre-fill UV flow cell. Make sure that the UV flow cell is pre-filled
with liquid before starting the system.
•
Avoid condensation. If ÄKTA start is kept in a cold room, cold
cabinet or similar, keep the instrument switched on in order to
avoid condensation.
•
Avoid overheating. If ÄKTA start is kept in a cold cabinet and
the cold cabinet is switched off, make sure to switch off the
instrument and keep the cold cabinet open to avoid overheating.
•
Place the computer in room temperature. If ÄKTA start is
placed in a cold room, place the computer outside the cold
room and use the PC Connectivity cable delivered with the instrument to connect to the computer.
•
Keep the pump cover open when not using the system. Open
the peristaltic pump cover after you switch off the equipment.
This will enhance the life time of the pump tubing.
ÄKTA start Operating Instructions 29-0270-57 AF
23
2 Safety instructions
2.1 Safety precautions
Maintenance
WARNING
To avoid damage to person when performing maintenance on
ÄKTA start, follow the instructions below.
•
Electrical shock hazard. Do not open any covers or parts unless
specified in the user documentation. Except for the maintenance and service described in the user documentation, all
other repairs should be done by service personnel authorized
by GE.
•
Only spare parts and accessories that are approved or supplied
by GE may be used for maintaining or servicing ÄKTA start.
•
Disconnect power. Always switch off power to the instrument
before replacing any component on the instrument or cleaning
the instrument, unless stated otherwise in the user documentation.
•
Spillage Hazard. Avoid spillage of fluids on the surfaces of the
instrument which have cables, plugs and other wirings. Be
careful if there is spillage of fluids on the tray while trying to
remove the tray from ÄKTA start.
•
NaOH is corrosive and therefore dangerous to health. When
using hazardous chemicals, avoid spillage and wear protective
glasses and other suitable Personal Protective Equipment (PPE).
CAUTION
To avoid damage to person when performing maintenance on
ÄKTA start, follow the instructions below.
24
•
Hazardous UV light. Always switch off power to the instrument
before replacing the UV flow cell.
•
If hazardous chemicals are used for system or column cleaning,
wash the system or columns with a neutral solution in the last
phase or step before maintenance.
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.1 Safety precautions
NOTICE
Cleaning. Keep the instrument dry and clean. Wipe regularly with
a soft damp tissue and, if necessary, a mild cleaning agent. Let the
instrument dry completely before use.
ÄKTA start Operating Instructions 29-0270-57 AF
25
2 Safety instructions
2.2 Labels
2.2
Labels
Introduction
This section describes the safety labels and labels concerning hazardous substances
that are attached to ÄKTA start. The instrument serial number is also visible from the
instrument product label which is illustrated here.
Labels on ÄKTA start
The illustrations below show the labels that are attached to ÄKTA start.
Label
Description
Placement
Instrument label including
safety symbols and specification.
Do not open any covers
on the instrument. This will
void the warranty.
Keep the pump cover
open when not using the
system. Open the pump
cover after you switch off
the instrument.
26
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.2 Labels
Label
Description
Placement
This product does not
comply with the ATEX
directive and must not be
used in explosive atmospheres.
Pinch hazard. Switch off
the Pump before tubing
loading.
Label on Frac30
The illustration below shows the labels that are attached to Frac30.
Label
Description
Placement
Instrument label including
safety symbols and specification.
Do not open any covers
on the instrument. This will
void the warranty.
ÄKTA start Operating Instructions 29-0270-57 AF
27
2 Safety instructions
2.2 Labels
Safety symbols
The following safety symbols are used in the labels:
Label
Meaning
Warning!
Do not use ÄKTA start before reading the ÄKTA start
Operating Instructions. Do not open any covers or replace parts unless specifically stated in the user documentation.
The system complies with the requirements for electromagnetic compliance (EMC) in Australia and New
Zealand.
The system complies with applicable European directives.
This symbol indicates that ÄKTA start has been certified
by a Nationally Recognized Testing Laboratory (NRTL).
NRTL means an organization that is recognized by the
US Occupational Safety and Health Administration (OSHA) as meeting the legal requirements of Title 29 of the
Code of Federal Regulations (29 CFR), Part 1910.7.
Labels concerning hazardous
substances
The following symbols on the labels concern hazardous substances:
Label
Meaning
This symbol indicates that electrical and electronic
equipment must not be disposed of as unsorted municipal waste and must be collected separately. Please
contact an authorized representative of the manufacturer for information concerning the decommissioning
of equipment.
28
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.2 Labels
Label
Meaning
This symbol indicates that the product contains hazardous materials in excess of the limits established by
the Chinese standard SJ/T11363-2006 Requirements
for Concentration Limits for Certain Hazardous Substances in Electronic Information Products.
ÄKTA start Operating Instructions 29-0270-57 AF
29
2 Safety instructions
2.3 Emergency procedures
2.3
Emergency procedures
Introduction
This section describes how to do an emergency shutdown of ÄKTA start. The section also
describes the result in the event of power failure.
Emergency shutdown
In an emergency situation:
Switch off power to the instrument by pressing the power switch to the O position or by
disconnecting the power cord from the instrument. The run is interrupted immediately.
WARNING
Access to power switch and power cord with plug. Do not block
access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
30
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.3 Emergency procedures
Power failure
The result of a power failure depends on which unit is affected.
Power failure to...
will result in...
ÄKTA start
•
The run is interrupted immediately.
•
The data collected up to the time of the power failure is available on the USB memory stick or, if the
system is connected to a computer, UNICORN start.
•
The computer with UNICORN start installed shuts
down.
•
On the ÄKTA start Instrument Display, all four touch
buttons will be highlighted.
•
The run is interrupted immediately.
•
Data generated up to 10 seconds before the power
failure can be recovered.
UNICORN start on a
computer
Note:
The UNICORN start client may close down during a
temporary overload of the processor. This may appear
as a computer failure. The run continues and you can
restart the UNICORN start client to regain control.
ÄKTA start Operating Instructions 29-0270-57 AF
31
2 Safety instructions
2.4 Recycling procedures
2.4
Recycling procedures
Introduction
This section describes the procedures for disposal and recycling of ÄKTA start.
Decommissioning of the
equipment
ÄKTA start must be decontaminated before decommissioning. Follow local regulations
for scrapping of the equipment.
Disposal, general instructions
When taking ÄKTA start out of service, the different materials must be separated and
recycled according to national and local environmental regulations.
Recycling of hazardous
substances
ÄKTA start contains hazardous substances. Detailed information is available from your
GE representative.
Disposal of electrical
components
Waste comprising electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning the decommissioning of
equipment.
32
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.5 Declaration of Hazardous Substances (DoHS)
2.5
Declaration of Hazardous Substances (DoHS)
Introduction
The following product pollution control information is provided according to SJ/T113642006 Marking for Control of Pollution caused by Electronic Information Products.
根据SJ/T11364-2006《电子信息产品污染控制标识要求》特提供如下有关污染 控制
方面的信息
Symbols used in pollution control
label
电子信息产品污染控制标志说明
Label
Meaning
This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard SJ/T11363-2006
Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Products. The number in the symbol
is the Environment-friendly Use Period (EFUP), which indicates the period
during which the toxic or hazardous substances or elements contained
in electronic information products will not leak or mutate under normal
operating conditions so that the use of such electronic information
products will not result in any severe environmental pollution, any
bodily injury or damage to any assets. The unit of the period is “Year”.
In order to maintain the declared EFUP, the product shall be operated
normally according to the instructions and environmental conditions
as defined in the product manual, and periodic maintenance schedules
specified in Product Maintenance Procedures shall be followed strictly.
Consumables or certain parts may have their own label with an EFUP
value less than the product. Periodic replacement of those consumables
or parts to maintain the declared EFUP shall be done in accordance
with the Product Maintenance Procedures.
This product must not be disposed of as unsorted municipal waste,
and must be collected separately and handled properly after decommissioning.
ÄKTA start Operating Instructions 29-0270-57 AF
33
2 Safety instructions
2.5 Declaration of Hazardous Substances (DoHS)
Label
Meaning
该标志表明本产品含有超过SJ/T11363-2006《电子信息产品中有毒
有害物质的限 量要求》中限量的有毒有害物质。标志中的数字为本
产品的环保使用期,表明本 产品在正常使用的条件下,有毒有害物
质不会发生外泄或突变,用户使用本产品 不会对环境造成严重污染
或对其人身、财产造成严重损害的期限。单位为年。
为保证所申明的环保使用期限,应按产品手册中所规定的环境条件
和方法进行正 常使用,并严格遵守产品维修手册中规定的期维修和
保养要求。
产品中的消耗件和某些零部件可能有其单独的环保使用期限标志,
并且其环保使 用期限有可能比整个产品本身的环保使用期限短。应
到期按产品维修程序更换那 些消耗件和零部件,以保证所申明的整
个产品的环保使用期限。
本产品在使用寿命结束时不可作为普通生活垃圾处理,应被单独收
集妥善处理
List of hazardous substances and
their concentrations
产品中有毒有害物质或元素的名称及含量
Indication for each major part if substance exceeds limit
Value
Meaning
O
Indicates that this toxic or hazardous substance contained in all of the
homogeneous materials for this part is below the limit requirement in
SJ/T11363-2006.
表示该有毒有害物质在该部件所有均质材料中的含量均在SJ/T113632006 标准规定的限量要 求以下
X
Indicates that this toxic or hazardous substance contained in at least
one of the homogeneous materials used for this part is above the limit
requirement in SJ/T11363-2006.
•
Data listed in the table represents best information available at the
time of publication
表示该有毒有害物质至少在该部件的某一均质材料中的含量超出
SJ/T11363-2006 标准规定的
限量要求
•
34
此表所列数据为发布时所能获得的最佳信息
ÄKTA start Operating Instructions 29-0270-57 AF
2 Safety instructions
2.5 Declaration of Hazardous Substances (DoHS)
List of hazardous substances
Component
name
Hazardous substance
有毒有害物质或元素
部件名称
1
Pb
Hg
Cd
Cr6+
PBB
PBDE
铅
汞
镉
六价铬
多溴联苯
多溴二苯醚
ÄKTA start,
29-0220-94 1
X
0
0
0
0
0
Frac30,
29-0230-511
X
0
0
0
0
0
UNICORN start,
29-0187-511
0
0
0
0
0
0
The product has not been tested as per the Chinese standard SJ/T11363-2006 Requirements
for Concentration Limits for Certain Hazardous Substances in Electronic Information Product.
ÄKTA start Operating Instructions 29-0270-57 AF
35
3 System description
3
System description
About this chapter
This chapter provides an overview of ÄKTA start, the Instrument Display, that allows the
user to operate and control the system, and Frac30 (Fraction collector).
In this chapter
This chapter contains the following sections:
Section
36
See page
3.1 System overview
37
3.2 Instrument
41
3.3 Instrument Display
44
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.1 System overview
3.1
System overview
Introduction
ÄKTA start is the main instrument included in a liquid chromatography system intended
primarily for preparative purification of proteins at laboratory-scale. The system can be
used for a variety of research purposes to fulfill the needs of the users in the academia
and in life sciences industry.
ÄKTA start is operated and controlled from the Instrument Display. In addition, the
UNICORN start software can be used to control ÄKTA start and to analyze the data acquired during chromatography runs. UNICORN start offers several additional features
that are described in detail in UNICORN start 1.0 User Manual.
This section gives an overview of the ÄKTA start System.
Illustration of the system
The illustration below shows the ÄKTA start System with UNICORN start installed on a
computer.
3
1
Part
Description
1
ÄKTA start (instrument).
2
Frac30 (Fraction collector).
3
UNICORN start (software installed on a computer).
ÄKTA start Operating Instructions 29-0270-57 AF
2
37
3 System description
3.1 System overview
Illustration of the instrument
The illustration below shows the main parts of the instrument.
3
1
5
4
2
38
Part
Description
Function
1
Instrument Display
User interface for controlling the system and
visualization of the runtime data.
2
Wet side
The modules interconnected by tubing have
the following functions:
•
to deliver the liquid in a specified flow path
and divert the flow as required,
•
to monitor the UV absorbance and
conductivity of the liquid.
3
Buffer tray
Location intended for the placement of buffer
bottles used during chromatography runs.
4
Power switch
Connects or disconnects the power.
5
USB port
To connect a USB memory stick for storage of
results and transfer of files.
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.1 System overview
Illustration of the Fraction
collector
The illustration below shows the Fraction collector; Frac30.
2
1
3
4
5
6
Part
Description
Function
1
Dispenser arm
assembly
Holds and positions the tubing holder for dispensing the liquid into fractions.
2
Tubing holder
Holds the tubing used for dispensing the liquid
fractions into the collection tubes.
3
Collection tubes
10 to 18 mm diameter tubes used to collect the
fractions.
4
Bowl assembly
Holder for collection tubes, which supports tubes
of four sizes.
5
Base unit
Case for electromechanical assembly and holder
for the Bowl assembly.
6
LED
Power on indicator.
ÄKTA start Operating Instructions 29-0270-57 AF
39
3 System description
3.1 System overview
Main features of ÄKTA start
The main features of ÄKTA start are listed below:
40
•
ÄKTA start is a compact and one step purification solution for quick and reliable purification of proteins.
•
A simple and modern system offered to automate the protein purification workflow
by providing features like automated sample injection, fraction collection, real-time
monitoring.
•
Method templates are available for all common chromatography techniques such
as Affinity Chromatography, Ion Exchange Chromatography, Gel filtration, and Desalting.
•
Quick start methods are available for purifying several common proteins.
•
Predefined system methods are available for cleaning the flow path.
•
ÄKTA start is operated using a touch screen on the instrument.
•
In addition, the system can be operated from a computer connected to the instrument
using the UNICORN start software.
•
ÄKTA start is offered with a dedicated Fraction collector, Frac30, allowing to collect
the fractions in four different tube sizes.
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.2 Instrument
3.2
Instrument
Introduction
This section provides an overview of ÄKTA start.
Illustration of the instrument
modules
The illustration below shows the locations and gives brief descriptions of the modules
placed on the wet side of the instrument.
8
9
10
1
2
7
3
6
4
5
Part
Function
Description
1
Buffer valve
A 3-port valve that is used as a switching valve
for gradient formation. It enables the use of two
buffers, which are required for forming a gradient
during runs.
ÄKTA start Operating Instructions 29-0270-57 AF
41
3 System description
3.2 Instrument
Part
Function
Description
2
Mixer
A static mixer that is used for mixing of buffers A
and B.
3
Sample valve
A 3-port valve that allows either the buffer or the
sample to enter the flow path. The Sample valve
enables direct application of the sample onto the
column using the Pump.
4
Pump
A peristaltic pump, which delivers buffer or sample
to the flow path with a flow rate of up to 5 ml/min.
For cleaning procedures, the Pump can flush the
flow path at a flow rate of 10 ml/min.
5
Pressure sensor
The Pressure sensor reads the pressure in the
flow path and senses overpressure.
6
Wash valve
A 3-port valve that is used to divert the flow path
to waste. The Wash valve switches automatically
during the predefined cleaning procedure, Pump
wash. In a manual run, the valve can be set to the
intended position by configuring the run
parameters.
7
Injection valve
A 6-port manually operated valve that is used to
transfer the sample loaded in the sample loop on
to the column.
A sample loop is connected to the appropriate
ports of the valve. The valve is switched manually
to positions:
8
42
UV
•
Load sample (default): to allow the loading of
the sample into the sample loop.
•
Inject to column: to transfer the sample from
the loop on to the column during a
chromatography run.
The UV Monitor continuously measures the absorbance of the liquid in the UV flow cell at a set
wavelength of 280 nm. The UV flow cell has a
path length of 2 mm.
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.2 Instrument
Part
Function
Description
9
Conductivity
The Conductivity Monitor continuously reads the
conductivity of the liquid in the Conductivity flow
cell.
The conductivity is automatically calculated by
multiplying the measured conductance by the cell
constant of the flow cell. The cell constant is
factory-calibrated.
The Conductivity flow cell is provided with a
temperature sensor that measures the temperature of the liquid in the Conductivity flow cell.
Note:
The buffers used should be within the conductivity
range of the instrument (0 to 300 mS/cm).
10
Outlet valve
ÄKTA start Operating Instructions 29-0270-57 AF
A 3-port valve that is used to direct the flow to the
Fraction collector, or to Waste.
43
3 System description
3.3 Instrument Display
3.3
Instrument Display
Introduction
This section provides a description of the ÄKTA start Instrument Display and the functions
that are accessible from the display.
In this section
This section contains the following sub-sections:
Section
44
See page
3.3.1 Overview of the Instrument Display
45
3.3.2 Description of Method run
50
3.3.3 Description of Create method
53
3.3.4 Description of Settings and service
54
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.1 Overview of the Instrument Display
3.3.1
Overview of the Instrument Display
Introduction
The Instrument Display is located on the front side of ÄKTA start. The Instrument Display
enables the user to control the system by selecting the intended operation:
•
Start a run and control an ongoing run.
•
View the progress of the ongoing run.
•
Manage user defined methods.
•
Perform maintenance and service.
This section provides a brief description of the functions of the Instrument Display.
Note:
Do not operate the Instrument Display with a sharp and hard object.
Illustration of the Instrument
Display
The illustration below shows the location and detailed view of the Instrument Display.
1
2
3
Part
Description
1
Screen name area.
2
Information area. The example shows the ÄKTA start home screen.
3
Help button.
ÄKTA start Operating Instructions 29-0270-57 AF
45
3 System description
3.3 Instrument Display
3.3.1 Overview of the Instrument Display
Instrument software
ÄKTA start offers the following functionality menu, displayed on the ÄKTA start home
screen as presented in the table below. For detailed workflows on the different options,
see Chapter 6 Operations from the Instrument Display, on page 158.
Option
Description
Method run
Use a quick start or a method template to perform
a run.
Manual run
Perform a run by providing parameters manually.
Create method
Create, edit, import and delete user methods.
Settings and service
Configure settings and calibrate modules, perform
diagnostics tests.
Description of buttons
on the Instrument Display
The Instrument Display includes the following touch buttons:
Button
46
Name
Description
Help
Opens a new dialog screen, providing information
about the content of the current screen or indicates where further information or instructions
can be found.
Home
Opens the ÄKTA start home screen.
Forward
Opens the next screen in the current workflow.
Return
Opens the previous screen in the current workflow.
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.1 Overview of the Instrument Display
Button
Name
Description
Increment (up
arrow)
The value in the text field can be increased or decreased by tapping up or down arrow.
Decrement
(down arrow)
Tapping the value opens the numpad and a new
value can be typed in.
Note:
After typing in a value on the numpad, tap OK to
confirm the new value.
Next
Opens the next screen.
Back
Returns to the previous screen.
Run
Starts a run.
Pause
Pauses the ongoing run by stopping the Pump.
The flow rate settings and the gradient values are
retained.
Continue
Continues a run that has been paused.
Hold
Holds an ongoing run, with current flow rate, valve
positions, and with set %B concentration. The
gradient is held at the value displayed.
Resume
Resumes a run that is put on hold.
Edit run
Opens a new screen for editing the current run
parameters.
ÄKTA start Operating Instructions 29-0270-57 AF
47
3 System description
3.3 Instrument Display
3.3.1 Overview of the Instrument Display
Button
48
Name
Description
Execute
Executes the edited run parameters during a run.
OK
Confirms a selection or an action.
Cancel
Cancels a selection or an action.
End
Terminates the ongoing run; always followed by
a screen that requires to confirm the action.
Exit
When a run is completed, it closes the application
and returns to the ÄKTA start home screen.
Graph icon
Opens the graphic view of the run in progress,
displaying the plot of the UV absorbance (mAU)
versus Time (min).
Save
Saves a user method that was either created or
edited.
Select
Confirms and initiates a run from a specific template or user method.
Create
Creates a user method based on selected template. The run parameters have to be edited as
needed.
Yes
Confirms an action.
No
Rejects an action.
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.1 Overview of the Instrument Display
Description of Instrument
Display Help
The ÄKTA start Instrument Display Help is accessible from every screen on the Instrument
Display, by tapping the question mark in the upper right corner. The Help text provides
information about the content of the current screen or refers to more detailed documentation.
ÄKTA start Operating Instructions 29-0270-57 AF
49
3 System description
3.3 Instrument Display
3.3.2 Description of Method run
3.3.2
Description of Method run
Method run options
The display option Method run allows the user to run methods based on Quick start
techniques or predefined method templates, user created methods, and run predefined
methods such as Pump Wash, and System cleaning. Detailed instructions to run
methods are presented in Section 6.4 Perform a method run, on page 173.
When Method run is selected, further options are displayed in the Method run screen.
The method types available under Method run are briefly described below.
Method
Description
Quick start (1/3)
Displays the Quick start templates
available with ÄKTA start.
For description of the Quick start
methods, see Section 6.4.2 Quick
start, on page 176.
Quick start (2/3)
50
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.2 Description of Method run
Method
Description
Quick start (3/3)
Templates
Displays the method templates for
chromatography techniques available with ÄKTA start.
For a description of the templates,
see Section 6.4.3 Templates, on
page 182.
User defined
Displays the methods created by the
user.
For detailed instructions on creating
and managing user defined methods, see Section 6.6 Manage methods
and files, on page 198.
Prepare system (1/2)
Displays the predefined system
methods that are used to clean the
system flow path.
For detailed instructions on running
system methods, see Section 6.4.5
Prepare system methods, on page192.
ÄKTA start Operating Instructions 29-0270-57 AF
51
3 System description
3.3 Instrument Display
3.3.2 Description of Method run
Method
Description
Prepare system (2/2)
52
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.3 Description of Create method
3.3.3
Description of Create method
Create method options
The display option Create method allows the user to create new methods, edit or delete
existing user methods and also import methods stored on a USB memory stick connected
to the instrument.
When Create method is selected, further options are displayed in the Create method
screen.
The operations available under Create method are briefly described below.
Option
Description
Create
Displays the method templates that can be used to create a new
method.
Edit
Displays the user methods stored on the instrument that can be
edited, if required.
USB Import
Displays a list of user methods stored on a USB memory stick that
can be imported to the instrument.
Delete
Displays the user methods stored on the instrument that can be
deleted, if required.
ÄKTA start Operating Instructions 29-0270-57 AF
53
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
3.3.4
Description of Settings and service
Settings and service options
The display option Settings and service allows the user to perform maintenance,
calibration, setting the delay volume, diagnostics and troubleshooting of the modules
located on the wet side of the instrument. For a brief description of the modules, see
Section 3.2 Instrument, on page 41. Detailed instructions for calibrating the Pump, the
monitors, and the Instrument Display are presented in Section 5.3 Calibrations, on page 91.
The options available under Settings and service are listed in the table below.
Option
Function
Settings and service - Screen 1
Allows the user to perform calibration, set the delay volume, and diagnostics and/or troubleshooting. The
menu covers the following modules:
Settings and service - Screen 2
Settings and service - Screen 3
54
•
Fraction collector (Fraction collector)
•
Pressure sensor
•
Pump
•
System (System)
Allows the user to perform diagnostics and/or troubleshooting for the
modules:
•
Buffer valve
•
Sample valve
•
Wash valve
•
Outlet valve
Allows the user to perform calibration, diagnostics, configuration
and/or troubleshooting for the modules:
•
Main board (Main board)
•
UV
•
Display (Display)
•
Conductivity
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Settings and service - Screen 1
The options available for the modules displayed on Settings and service Screen 1 are
briefly described below.
Module
Function
Fraction collector (Fraction collector)
Enable Frac/Disable Frac: Activates/Deactivates the Fraction collector connection.
Diagnostics: Performs a feed tube
test that checks if the Bowl assembly
rotates correctly. Performs a Home
test to verify that the Bowl assembly
returns to Home position.
Run log: To see the number of hours
the Frac drive has been active and
to reset the number of hours of drive
usage.
Pressure sensor
Zero offset: Allows to set the pressure to zero. The Pump has to be off
and the flow path connection to the
Pressure sensor has to be open.
P set: Shows the pressure in the flow
path.
Note:
Pressure Calibration is performed by
a GE Healthcare Service Engineer
only, after the Pressure sensor is replaced.
ÄKTA start Operating Instructions 29-0270-57 AF
55
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Module
Function
Pump
Calibration: To calibrate the Pump,
so that it delivers liquid with an accurate flow rate.
Pump tubing log:
•
To note the number of hours the
pump tubing has been in use
(Tubing run) and to reset the
count of tubing usage (after the
pump tubing is replaced).
•
To note the number of hours the
Pump has been in use (Pump
run) and to reset the count of
Pump usage (after the Pump is
replaced).
Diagnostics: To adjust the flow rate
and start/stop the flow (Pump).
56
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Module
Function
System (System)
Delay volume setting: To set the
delay volume. The delay volume
represents the volume of the liquid
found in the flow path between the
UV and the collection tubes.
Firmware update: To update the
system firmware.
Switch valve timing: To set the
Switch valve timing. It is recommended to optimize the timing of switch
valve (Buffer valve) when wavy gradients are obtained or when fluctuations in the step gradient are observed during either system performance tests or chromatography
runs.
Export system report to USB:
Exports system error logs to a USB
memory stick in a text format. A
system error report is useful when
trying to understand a possible error
that requires remote service support.
Note:
The serial number displayed on the
top left of the display screen is the
unique identifier for the instrument.
The number is also seen as instrument ID when connecting to
UNICORN start.
ÄKTA start Operating Instructions 29-0270-57 AF
57
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Settings and service - Screen 2
The options available for the modules displayed on Settings and service Screen 2 are
briefly described below.
Module
Function
Buffer valve
Valve position: Shows which inlet
connection in the valve is open,
Buffer A (Buffer A) or Buffer B (Buffer
B).
Turn valve: Switches the valve between its two positions i.e., the A and
B inlet ports.
Valve switches: The text box displays
the number of counts the valve has
switched.
Reset: Resets the counts to zero
when a new valve has been replaced.
Sample valve
Valve position: Shows which inlet
connection in the valve is open,
Buffer (Buffer) or Sample (Sample).
Turn valve: Switches the valve between its two positions i.e., the Buffer
and Sample inlet ports.
Valve switches: The text box displays
the number of counts the valve has
switched.
Reset: Resets the counts to zero
when a new valve has been replaced.
58
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Module
Function
Wash valve
Valve position: Shows which outlet
connection in the valve is open,
Waste (Waste) or Column (Column).
Turn valve: Switches the valve between its two positions i.e., the
Waste and Column outlet ports.
Valve switches: The text box displays
the number of counts the valve has
switched.
Reset: Resets the counts to zero
when a new valve has been replaced.
Outlet valve
Valve position: Shows which outlet
connection in the valve is open,
Waste (Waste) or Collection (Collection).
Turn valve: Switches the valve between its two positions i.e., the
Waste and Collection/Frac outlet
ports.
Valve switches: The text box displays
the number of counts the valve has
switched.
Reset: Resets the counts to zero
when a new valve has been replaced.
ÄKTA start Operating Instructions 29-0270-57 AF
59
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Settings and service - Screen 3
The options available for the modules displayed on Settings and service Screen 3 are
briefly described below.
Module
Function
Main board (Main board)
Power supply: Shows the actual
voltage.
Memory test: Performs a memory
test.
Temperature: Shows the temperature of the main board.
FPGA test: Performs a test of the
FPGA hardware.
UV
UV LED calibration: To calibrate
theUV LED intensity.
Flow cell path length: To derive the
actual path length of the flow cell.
Diagnostics: To troubleshoot the UV
module.
Configuration: To configure the new
UV monitor and flow cell.
Note:
The C amb and C drft values should
not be altered by the user. UV Configuration is performed by a GE
Healthcare Service Engineer only.
Display (Display)
Touch screen calibration: To calibrate the touch screen.
Color test: To perform a diagnostics
test for the colors displayed on the
touch screen.
Diagnostics: To perform a diagnostics test to check the backlight performance of the display.
Log book: To read the number of
hours the instrument display has
been in use.
60
ÄKTA start Operating Instructions 29-0270-57 AF
3 System description
3.3 Instrument Display
3.3.4 Description of Settings and service
Module
Function
Conductivity
Calibration: To calibrate the Conductivity flow cell and the temperature
sensor.
Configuration: To set a new cell
constant value or reference temperature value, usually after the Conductivity flow cell replacement.
Advanced calibration: To calibrate
the temperature of the Conductivity
flow cell.
Note:
Calibration of the Sine Wave Generator should be performed by a GE
Service Engineer only, after the Main
board has been replaced.
ÄKTA start Operating Instructions 29-0270-57 AF
61
4 Installation
4
Installation
About this chapter
This chapter provides the necessary instructions to enable users to unpack and install
ÄKTA start and Frac30. Read the entire Installation chapter before starting to install ÄKTA
start.
In this chapter
This chapter contains the following sections:
Section
62
See page
4.1 Space requirements
63
4.2 Transport ÄKTA start and Frac30
65
4.3 Unpack ÄKTA start and Frac30
67
4.4 Accessories package
77
4.5 Install ÄKTA start
78
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.1 Space requirements
4.1
Space requirements
Benchtop setup
The illustration below shows the space requirements recommended for ÄKTA start.
80 cm
34 cm
80 cm
ÄKTA start Operating Instructions 29-0270-57 AF
27 cm
100 cm
63
4 Installation
4.1 Space requirements
Equipment dimensions
ÄKTA start
360 mm
280 mm
340 mm
Frac30
285 mm
280 mm
270 mm
64
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.2 Transport ÄKTA start and Frac30
4.2
Transport ÄKTA start and Frac30
Equipment weight
Item
Weight
ÄKTA start (with packaging)
12 kg
Frac30 (with packaging)
6 kg
Handling the delivery boxes
ÄKTA start and Frac30 are packed in two separate boxes.
To transport the delivery boxes containing the instrument and Fraction collector, use a
hand truck suitable for light weight packages. However, each box can be lifted by 1
person without the help of any lifting equipment.
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4 Installation
4.2 Transport ÄKTA start and Frac30
66
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.3 Unpack ÄKTA start and Frac30
4.3
Unpack ÄKTA start and Frac30
Introduction
This section describes how to unpack ÄKTA start and Frac30.
Note:
Save all the original packing material. If the system has to be repacked, for
transportation or otherwise, it is important that the system can be safely
packed using the original packing material.
Unpack ÄKTA start
Follow the instructions below to unpack the instrument.
CAUTION
ÄKTA start is filled with denaturated alcohol (50% C2H5OH (ethanol))
at delivery. The denatured alcohol mixture can be hazardous to
humans if consumed. Flush out the denaturated alcohol before
assembling, testing or integrating ÄKTA start into the intended
process context.
Note:
ÄKTA start with packaging weighs about 12 kg. No lifting equipment required,
one person can lift and move the instrument.
Step
Action
1
Open the delivery box by cutting the adhesive tape at the top of the box.
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67
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
2
Take out the document placed at the top of the package and read the Unpacking Instructions.
Note:
Save the documents for future reference.
3
68
Take out the box placed at the top of the package. The box contains the
accessories delivered with the instrument.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
4
Hold the red strap, and then lift the instrument out of the delivery box.
5
Open the strap lock and remove the strap.
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4 Installation
4.3 Unpack ÄKTA start and Frac30
70
Step
Action
6
Remove the foam cushion from the top of the instrument.
7
Remove the foam cushion from the bottom of the instrument by carefully
lifting the instrument.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
8
Remove the plastic bag by gently tilting the system back and forth while
pulling out the plastic bag.
Unpack Frac30
Follow the instructions below to unpack the Fraction collector.
CAUTION
Take care not to damage the Dispenser arm when lifting Frac30
or when removing the plastic bag.
NOTICE
Never lift the Frac30 by the Dispenser arm. This may damage the
Fraction collector.
Note:
Frac30 with packaging weighs about 6 kg. No lifting equipment required, one
person can lift and move the Fraction collector.
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71
4 Installation
4.3 Unpack ÄKTA start and Frac30
72
Step
Action
1
Open the Frac30 delivery box by cutting the adhesive tape at the top of the
box.
2
Take out the document placed at the top of the package and read the Unpacking Instructions.
3
Holding the red strap, lift the fraction collector out of the delivery box. Place
the Fraction collector on the laboratory bench.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
4
Open the strap lock and remove the strap.
5
Remove the foam cushion from the top of the Fraction collector.
ÄKTA start Operating Instructions 29-0270-57 AF
73
4 Installation
4.3 Unpack ÄKTA start and Frac30
74
Step
Action
6
Remove the foam cushion from the bottom of the fraction collector by
carefully lifting the Fraction collector.
7
Remove the plastic bag.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
8
Remove the Bowl assembly from the Base unit:
9
•
Gently move the Dispenser arm counterclockwise to the end position.
•
Push the Drive assembly and hold it at the retracted position. At the
same time, lift the Bowl assembly.
Remove the foam cushion located on the Base unit.
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75
4 Installation
4.3 Unpack ÄKTA start and Frac30
Step
Action
10
Re-assemble the Bowl assembly on to the Base unit:
•
Orient the Bowl to match the aligning groove and the aligning features
located on the bowl holder.
•
Slightly push the Drive assembly laterally and lower the Bowl assembly
onto the Base unit.
NOTICE
Never use the Dispenser arm assembly to lift or hold Frac30. To lift
the module, use the handle provided on the bottom plate.
Note:
76
Do not damage or break the warranty seal label during unpacking of Frac30.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.4 Accessories package
4.4
Accessories package
Illustration of the accessories
package
The illustration below shows the accessories box and the user documentation included
with ÄKTA start at delivery.
ÄKTA start
2
3
1
4
5
6
7
Part
Description
1
Unpacking Instructions
2
System certificate
3
Product documentation
4
Maintenance Cue Card
5
System Cue Card
6
CD containing user documentation files and an unpacking video.
The CD includes Operating Instructions and Maintenance Manual in English
and translated versions.
7
Accessories box
ÄKTA start Operating Instructions 29-0270-57 AF
77
4 Installation
4.5 Install ÄKTA start
4.5
Install ÄKTA start
Introduction
This section describes how to install ÄKTA start. The following actions must be performed:
•
Install pump tubing.
•
Connect power supply to ÄKTA start.
•
Connect Frac30 to ÄKTA start.
•
Connect ÄKTA start to the UNICORN start computer.
WARNING
Only use grounded power cords delivered or approved by GE.
WARNING
Supply voltage. Make sure that the supply voltage at the wall
outlet corresponds to the marking on the instrument, before connecting the power cord.
CAUTION
Protective ground. ÄKTA start must always be connected to a
grounded power outlet.
78
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.5 Install ÄKTA start
Install pump tubing
Follow the instructions below to install the pump tubing.
Step
Action
1
Open the top cover until it is fully open.
2
Place the tubing between the rollers and the track, press against the pump
head inner wall.
Note:
Make sure that the pump tubing is not twisted or stretched against the rollers.
ÄKTA start Operating Instructions 29-0270-57 AF
79
4 Installation
4.5 Install ÄKTA start
Step
Action
3
Lower the top cover until it clicks into its fully closed position.
The track closes automatically and the tubing is stretched correctly as the
track closes.
Connect power to ÄKTA start
Follow the instructions below to connect power to ÄKTA start.
Step
Action
1
Select the correct power cord to be used. ÄKTA start is delivered with 2 alternative power cords:
•
Power cord with US-plug, 2 m.
•
Power cord with EU-plug, 2 m.
Note:
Discard the unused power cord.
80
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.5 Install ÄKTA start
Step
Action
2
Connect the power cord to the Power input connector on the left side of the
instrument and to a grounded wall outlet 100-240 VAC, 50/60 Hz.
CAUTION
ÄKTA start is filled with denaturated alcohol (50%
C2H5OH (ethanol)) at delivery. The denatured alcohol
mixture can be hazardous to humans if consumed.
Flush out the denaturated alcohol before assembling,
testing or integrating ÄKTA start into the intended
process context.
Note:
For instructions on how to clean the flow path, see Section 8.3 Cleaning the
system flow path, on page 224.
Connect Frac30 to ÄKTA start
NOTICE
Frac30 should not be connected or disconnected from ÄKTA start
when the instrument is powered ON.
Follow the instructions below to connect Frac30 to ÄKTA start.
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81
4 Installation
4.5 Install ÄKTA start
Step
Action
1
Connect the Frac30 cable between the intended ports on the back of the
Fraction collector and the instrument.
Note:
The supply voltage for Frac30 is distributed from ÄKTA start.
When the Frac30 cable is connected, the screws attached to the connector
should be tightened.
2
Switch on ÄKTA start.
3
Enable the connection of Frac30 from the Instrument Display:
•
In the ÄKTA start home screen, tap Settings and service.
Result: The Settings and service Screen 1 opens.
82
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4 Installation
4.5 Install ÄKTA start
Step
Action
•
In the Settings and service screen, tap Fraction collector.
Result: The Fraction collector screen opens.
•
In the Fraction collector screen, tap Enable Frac to enable the connection of the Fraction collector.
Result: The following screen will be displayed.
Note:
The power on status of the Fraction collector is indicated by the white LED
on the front of Frac30.
Connect a computer to ÄKTA
start
Note:
Before connecting the computer to ÄKTA start, install the UNICORN start software on the computer. Refer to the UNICORN start 1.0 User Manual.
Follow the instructions below to connect a UNICORN start computer to ÄKTA start.
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83
4 Installation
4.5 Install ÄKTA start
Step
Action
1
Connect power to the computer and monitor, and then switch on the computer and ÄKTA start.
Result: The instrument displays the ÄKTA start home screen.
2
84
Connect the PC Connection Cable between the connector marked as PC
Connection on the back of ÄKTA start and a USB port on the computer.
ÄKTA start Operating Instructions 29-0270-57 AF
4 Installation
4.5 Install ÄKTA start
Step
Action
3
Launch UNICORN start and connect to ÄKTA start. For detailed instructions,
refer to UNICORN start 1.0 User Manual.
Result: The instrument displays the ÄKTA start home screen in connected
state.
Note:
Make sure that the system connection is established before starting the run.
Always make sure to be in the ÄKTA start home screen (connected state)
when trying to connect from the System Control module.
CAUTION
ÄKTA start is filled with denaturated alcohol (50% C2H5OH (ethanol))
at delivery. The denatured alcohol mixture can be hazardous to
humans if consumed. Flush out the denaturated alcohol before
assembling, testing or integrating ÄKTA start into the intended
process context.
ÄKTA start Operating Instructions 29-0270-57 AF
85
5 Prepare the system for a run
5
Prepare the system for a run
About this chapter
This chapter describes how to start the instrument and prepare the system for a run.
In this chapter
This chapter contains the following sections:
Section
86
See page
5.1 Flow path overview
87
5.2 Start the instrument
90
5.3 Calibrations
91
5.4 System performance
109
5.5 Connect a column
124
5.6 Run Prepare system methods
128
5.7 Sample application
138
5.8 Prepare the Fraction collector
150
5.9 Cold room operations
154
5.10 Starting a run
156
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.1 Flow path overview
5.1
Flow path overview
Illustration of the flow path
The illustration below shows the flow path for ÄKTA start. The flow path contains Pump,
Mixer, Valves, and UV, Conductivity and Pressure monitors. The individual instrument
modules are presented in the table below. For a detailed description of the modules, see
the ÄKTA start Maintenance Manual.
Part
Description
Part
Description
1
Buffer valve
7
Injection valve (manual)
2
Mixer
8
Column
3
Sample valve
9
UV Monitor
4
Pump
10
Conductivity Monitor
5
Pressure sensor
11
Outlet valve
6
Wash valve
12
Fraction collector
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5 Prepare the system for a run
5.1 Flow path overview
Inlet and outlet tubing
ÄKTA start is delivered with the entire flow path assembled and pre-filled with storage
solution (50% Ethanol). Details on the tubing types used along the flow path are presented
in Chapter 10 Reference information, on page 260.
The table below lists the tubing connected to the instrument. Prepare the system for a
run by connecting inlet and outlet tubing to the valve ports marked with orange arrows.
Module
Tube connection
Scope of use
Buffer valve
Port I (Buffer A)
Inlet tubing for buffer A
Port II
Outlet tubing to the Mixer.
Port III (Buffer B)
Inlet tubing for buffer B
Port I (Sample)
Inlet tubing used when the sample is applied
via the Pump.
Port II
Pump tubing.
Port III
Inlet tubing from the Mixer.
Port I (Waste)
Outlet tubing used when cleaning the flow
path or changing the buffer by running the
Pump Wash A/B template.
Port II
Inlet tubing from the Pressure sensor.
Port III
Outlet tubing to the Injection valve
Port 1
Outlet; the tubing is connected to the column.
Ports 2 and 5
Inlet/outlet for connecting the sample loop.
Port 3
Inlet for injecting the sample into the loop.
Port 4 (Waste)
Outlet tubing to waste, helps in washing or
draining excess sample from the loop.
Port 6
Inlet; the tubing is connected to Wash valve.
Port I (Waste)
Outlet tubing to the waste container.
Port II
Inlet tubing from the Conductivity Monitor.
Port III (Collection)
Outlet tubing to the Fraction collector.
Sample valve
Wash valve
Injection
valve
Outlet valve
88
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5 Prepare the system for a run
5.1 Flow path overview
Placement of buffer bottles
Buffer bottles are placed in the Buffer tray on top of the instrument, as illustrated below.
Sample bottle or tube may be placed on the bench on the left side of the instrument. A
waste bottle may be placed on the bench on the right side of the instrument.
CAUTION
Max. weight on Buffer tray. Do not place bottles with a volume of
more than 1 liter each on the Buffer tray. The total allowed weight
on the Buffer tray is 5 kg.
CAUTION
Avoid spillage and overflow. Make sure that the waste tubing is
inserted in an appropriate waste container and secured in place.
ÄKTA start Operating Instructions 29-0270-57 AF
89
5 Prepare the system for a run
5.2 Start the instrument
5.2
Start the instrument
Switch on the instrument
Follow the instruction below to start the instrument.
Step
Action
1
Switch on the instrument by pressing the Power switch to the l position.
Result: The instrument starts and initializes the display, showing the ÄKTA
start home screen.
2
90
You can start using the instrument immediately. All modules are pre-calibrated from the factory.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3
Calibrations
Introduction
This section describes how to calibrate the Instrument Display, Pump, and Monitors.
In this section
This section contains the following subsections:
Section
See page
5.3.1 Calibration guide
92
5.3.2 Instrument Display calibration
93
5.3.3 Pressure sensor zero offset
95
5.3.4 Pump calibration
97
5.3.5 UV Monitor calibration
99
5.3.6 Conductivity Monitor calibration
ÄKTA start Operating Instructions 29-0270-57 AF
103
91
5 Prepare the system for a run
5.3 Calibrations
5.3.1 Calibration guide
5.3.1
Calibration guide
When to calibrate
Note:
The instrument is pre-calibrated at delivery, therefore no calibration is required
when the instrument is installed. However, if the System performance test
fails it is recommended to calibrate the modules.
The table below provides recommendations for when modules should be calibrated.
Module
When to calibrate
Instrument Display
•
If there are any issues with the response of
the touch screen.
Pressure sensor
•
If the pressure is outside the range of ± 0.03
MPa, perform Zero offset.
Pump
•
When chromatography run conditions are
changed, e.g., viscosity of sample or buffer,
temperature, back-pressure.
•
Pump and pump tubing requires calibration
regularly. Recommended: once a week.
•
After the pump tubing has been replaced with
new tubing.
Note:
Do not leave the pump tubing inside the Pump
when the Pump is not running.
UV Monitor
Conductivity Monitor
92
•
When the signal is unstable, or readings
appear to be incorrect.
•
After cleaning, or after replacing the UV flow
cell.
•
When error/warning is seen on power ON.
•
When baselining is ignored when the UV flow
cell is clean.
•
Before and after performing runs in the cold
room.
•
When the signal is unstable, or readings
appear to be incorrect.
•
After replacing the Conductivity flow cell.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3.2 Instrument Display calibration
5.3.2
Instrument Display calibration
Instruction
Follow the instructions below to calibrate the Instrument Display.
Step
Action
1
In the ÄKTA start home screen, tap Settings and service to access the instrument modules. Tap Next:Next to access the 3rd screen.
Result: The Settings and service screen will be displayed.
2
In the Settings and service screen, tap Display to access the touch screen
options.
Result: The Display screen opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.2 Instrument Display calibration
Step
Action
3
In the Display screen, tap Touch screen calibration to start the calibration.
Result: A Message screen opens.
Tap Yes to confirm and proceed with the calibration
or
Tap No to cancel the action and return to the Display screen.
4
In the four subsequent Calibration screens, tap precisely on the marked
circles, as requested. The first Calibration screen is presented as an example.
5
Tap Exit when the calibration is completed.
Note:
Repeat the procedure if calibration fails.
94
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5 Prepare the system for a run
5.3 Calibrations
5.3.3 Pressure sensor zero offset
5.3.3
Pressure sensor zero offset
Zero offset
Follow the instructions below to set the pressure as zero when the Pressure sensor is
exposed to atmospheric pressure only (Zero offset).
Note:
Pressure Calibration is performed by a GE Healthcare Service Engineer only,
after the Pressure sensor is replaced.
Step
Action
1
Disconnect the inlet tubing from the Pressure sensor in order to expose the
sensor to atmospheric pressure only.
Note:
Make sure that the Pump is OFF before disconnecting the tubing.
2
In the ÄKTA start home screen, tap Settings and service to access the instrument modules.
Result: The Service and settings screen opens.
3
In the Settings and service screen, tap Pressure sensor to access the
Pressure sensor options.
Result: The Pressure sensor screen opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.3 Pressure sensor zero offset
Step
Action
4
In the Pressure sensor screen, tap Zero offset.
Result: A Message screen opens.
5
Tap OK if there is no back pressure in the system (i.e. the inlet tubing from
the Pump was disconnected from the Pressure sensor).
Result: The atmospheric pressure is set as zero.
6
96
Re-connect the inlet tubing from Pump to the Pressure sensor.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3.4 Pump calibration
5.3.4
Pump calibration
Instruction
Follow the instructions below to calibrate the Pump.
Step
Action
1
Immerse the buffer inlet tubing A in demineralized (DM) water.
2
Place the outlet tubing from the Wash valve in a pre-weighed collection
tube.
Note:
Before starting the calibration, prime the flow path with water and make sure
that the outlet tubing where the pumped water is collected is filled with DM
water. This ensures that the volume of the collected water corresponds to the
pumped volume.
3
In the ÄKTA start home screen, tap Settings and service to access the instrument modules.
Result: The Settings and service - Screen 1 opens.
4
In the Settings and service screen, tap Pump to access the Pump options.
Result: The Pump screen opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.4 Pump calibration
Step
Action
5
In the Pump screen, tap Calibration.
Result: The Calibration screen opens.
6
In the Calibration screen, set the intended flow rate in the range 0.5 to 5
ml/min. Use the up/down arrows to set the value or use numpad to type in
the value.
7
In the Calibration screen, tap Start flow to start the Pump.
Result: The Pump starts and the pumped DM water will be collected in the
pre-weighed collection tube.
Collect water for at least one minute in the pre-weighed collection tube.
8
Estimate accurately the volume of the water collected in the pre-weighed
collection tube, then set the Collected volume value equal to this volume.
9
In the Calibration screen, tap Calibrate, then confirm the action (OK) to
carry out the calibration.
10
Verify that the Pump was correctly calibrated. Repeat the steps 6 and 7 to
confirm that the volume of the collected water corresponds to the intended
flow rate.
Note:
If the collected water does not correspond to the required volume (i.e., the
Pump does not deliver the liquid with the intended flow rate), inspect the
condition of the pump tubing. Replace the pump tubing if necessary, and then
perform re-calibration.
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5 Prepare the system for a run
5.3 Calibrations
5.3.5 UV Monitor calibration
5.3.5
UV Monitor calibration
Set the Cell path length
If the UV flow cell was replaced, a new Cell path length value has to be set.
Note:
Make sure that the UV flow cell is assembled tightly, and the inlet and outlet
tubing is fitted tightly to the UV Monitor. No stray light should enter the UV
Monitor. Make sure that there are no air bubbles in the UV flow cell.
Prerequisites
•
Buffer A: DM water
•
Buffer B: freshly prepared 1% acetone solution, which is expected to give an Abs
value of 340 mAU.
Follow the instructions below to set the Cell path length.
Step
Action
1
In the ÄKTA start home screen, tap Settings and service to access the instrument modules, then tap Next:Next.
Result: The Settings and service Screen 3 opens.
2
In the Settings and service screen, tap UV to access the UV Monitor options.
Result: The UV screen opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.5 UV Monitor calibration
Step
Action
3
In the UV screen, tap Flow cell path length to access the flow cell options.
For a description of the UV Monitor options, refer to the ÄKTA start
Maintenance Manual.
Result: The Flow Cell Path Length screen opens.
In the Flow Cell Path Length screen:
•
set the Cell path length in the Cell field, corresponding to the new UV
flow cell
Note:
The Cell path length is provided on the packing of the UV flow cell.
•
4
tap Save Path Length to save the value.
If the Cell path length is not available, follow the instructions below to calculate the Cell path length.
Immerse the inlet buffer A tubing in DM water and the inlet buffer B tubing
in 1% acetone solution.
5
In the Flow Cell path length screen, set the Cell value to 2.00 mm by using
the up/down arrows or typing in the value.
6
Flush thoroughly and leave the UV flow cell filled with buffer A (DM water).
Tap Set Baseline to capture a new reference for the test.
Result: Abs should show a value close to 0 mAU.
7
8
Flush the UV flow cell with Buffer B (1% acetone) and leave the flow cell
filled with 1% acetone. Note down the new Abs value.
Calculate the actual Cell path length:
Cell (mm) = 2.00 * (new Abs value / 340)
100
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5 Prepare the system for a run
5.3 Calibrations
5.3.5 UV Monitor calibration
Step
Action
9
Update the calculated Cell length value by using up/down buttons or typing
in the value. Tap Save Path Length to save the value to permanent memory.
Note:
Observe that the Abs value now should be about 340 ± 5% mAU, confirming
that normalization has been done.
Calibrate the UV LED
Follow the instructions below to calibrate the UV LED.
Note:
Make sure that the UV flow cell is assembled tightly, and the inlet and outlet
tubes are fitted tightly to the UV Monitor. No stray light should enter the UV
flow cell.
Step
Action
1
Immerse the buffer inlet tubing in DM water.
2
Flush the UV flow cell with DM water using the Pump. Make sure that there
are no air bubbles in the UV flow cell.
Note:
Flush thoroughly with DM water to make sure that the flow cell is clean.
3
In the ÄKTA start home screen, tap Settings and service to access the instrument modules, then tap Next:Next.
Result: The Settings and service Screen 3 opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.5 UV Monitor calibration
Step
Action
4
In the Settings and service screen, tap UV to access the UV Monitor options.
Result: The UV screen opens.
5
In the UV screen, tap UV LED calibration. For a description of the UV calibration parameters, refer to the ÄKTA start Maintenance Manual.
Result: The UV LED Calibration screen opens.
6
•
Set the Light strength value to 500 using up/down arrows or type in the
value.
•
Tap Calibrate. A confirmation screen opens. Select OK to automatically
search the Light strength value to get a minimum Signal response of
2500 mV.
•
If the Signal value is above 2500 mV, tap Save.
If the Signal value is below 2500 mV use the arrows to increase the Light
strength until the Signal is above 2500 mV.
7
102
Tap Save to store the calibrated Light strength value.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
5.3.6
Conductivity Monitor calibration
Set the Cell constant value
If the flow cell was replaced, a new Cell constant value has to be set. Follow the instructions below to set the Cell constant value.
Step
Action
1
In the ÄKTA start home screen, tap Settings and service to access the instrument modules, then tap Next:Next.
Result: The Settings and service screen 3 opens.
2
In the Settings and service screen, tap Conductivity to access the Conductivity Monitor options.
Result: The Conductivity screen opens.
3
In the Conductivity screen, tap Configuration to access the configuration
options.
Result: The Configuration screen opens.
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5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
Step
Action
4
In the Configuration screen, set the Cell Constant value in the Set Cell
Const field, for the new Conductivity flow cell by using the up/down arrows.
Tap Save to save the new value.
Note:
The Cell Constant value is provided on the packaging of the Conductivity
flow cell.
If a Cell constant value is not available, calibrate the Conductivity flow cell
as explained in Calibrate the Conductivity flow cell, on page 106.
Set the Reference Temperature
Note:
For the system to recalculate the measured conductivity provide the reference
temperature in the temperature field Set Ref Temp.
Make sure that the checkbox Enable Temperature Compensation in the
Conductivity Calibration screen is checked.
Follow the instructions below to set the reference temperature.
104
Step
Action
1
In the Conductivity:Configuration screen, set the Reference Temperature
(Set Ref Temp) value in the range 4°C to 35°C.
2
Tap Save to save the new reference temperature value.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
Calibrate the temperature
sensor
Follow the instructions below to calibrate the temperature sensor.
Step
Action
1
Place a precision thermometer in the path of the UV flow cell directly after
the Conductivity flow cell and pump DM water through system with a flow
rate of 0.5 ml/min.
2
In the Settings and service screen 3, tap Conductivity to access the Conductivity Monitor options.
Result: The Conductivity screen opens.
3
In the Conductivity screen, tap Advanced calibration.
Result: The Advanced calibration screen opens.
4
Note the temperature and type it into the Set Actual Temp field, then tap
Calibrate to carry out the temperature calibration.
Note:
Make sure that the temperature of the Conductivity flow cell has stabilized
and measure the temperature of the calibration solution with a precision
thermometer.
Note:
Calibrate Sine Gen is performed by a GE Healthcare Service Engineer only,
when the Main board is replaced.
ÄKTA start Operating Instructions 29-0270-57 AF
105
5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
Calibrate the Conductivity flow
cell
Prequisites
Calibration solution: 1.00 M NaCl or 100 mS/cm conductivity standard solution.
Follow the instructions below to calibrate the Conductivity flow cell.
Step
Action
1
In the Conductivity screen, tap Calibration.
Result: The Calibration screen opens.
2
Note the current temperature of the calibration solution in the Conductivity
flow cell as displayed in the Cell Temperature field.
3
In the Calibration screen, set the theoretical conductivity value at the current
temperature in the Set Conductivity field and then tap Calibrate to carry
out the conductivity calibration.
Note:
• If a certified conductivity standard solution is used, use the supplied theoretical conductivity value corresponding to the temperature in question.
•
106
If a manually prepared 1.00 M NaCl calibration solution is used, get the
conductivity value at the current temperature from the graph for conductivity of the 1.00 M NaCl as a function of temperature presented below.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
Graph for Conductivity value
The graph below shows the Conductivity value at the current temperature when 1.00
M NaCl calibration solution is used.
ÄKTA start Operating Instructions 29-0270-57 AF
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5 Prepare the system for a run
5.3 Calibrations
5.3.6 Conductivity Monitor calibration
108
Axis
Description
x
Temperature (°C)
y
Conductivity (mS/cm)
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4
System performance
Introduction
This section describes the System performance method and how to perform and evaluate the System performance method (system performance).
In this section
This section contains the following subsections:
Section
See page
5.4.1 System performance method
110
5.4.2 System performance method from ÄKTA start
112
5.4.3 System performance method from UNICORN start
117
5.4.4 Switch valve timing
120
ÄKTA start Operating Instructions 29-0270-57 AF
109
5 Prepare the system for a run
5.4 System performance
5.4.1 System performance method
5.4.1
System performance method
Introduction
The System performance method is performed to make sure that the system is performing within acceptable limits. It is recommended to run the test at the time of installation
of the instrument or after replacement of modules such as Pump, UV, Conductivity or
Valves. System performance method can also be used at any time to check the condition
of the system, for example, after a prolonged storage of the system. The System performance method can be performed from both the Instrument Display and UNICORN start.
Note:
•
Calibrate all the modules before starting the test.
•
Make sure that there is no column connected.
•
It is recommended not to edit any run parameters during a test in order
to avoid the failure of the test.
Requirements
Required solutions are:
•
Buffer A - DM water
•
Buffer B - 1.0% acetone, 1.0 M NaCl
•
Sample - 1.0% acetone, 1.0 M NaCl (Buffer B)
Note:
Make sure to prepare the buffer solutions accurately to avoid the test failure.
Checklist
Before starting a System performance method, make sure that the following tasks are
completed or met:
110
•
Calibration of all the modules: Pressure sensor, Pump, UV and Conductivity.
•
No column should be present in the flow path.
•
Set the conductivity reference temperature to 20°C, save and enable the function.
•
Immerse Buffer port A inlet in Buffer A (DM water).
•
Immerse Buffer port B inlet in Buffer B (1.0% acetone, 1.0 M NaCl).
•
Sample valve inlet immersed in sample (1.0% acetone, 1.0 M NaCl).
•
Make sure that 1 ml Sample loop is filled with sample (1.0% acetone, 1.0 M NaCl).
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.1 System performance method
•
Make sure that 2 m of 0.5 mm ID tubing is connected to the Outlet valve at Waste
position.
•
When performing System performance method without Fraction collector, make
sure the Outlet valve fractionation tubing is inserted into a pre-weighed beaker.
•
When performing System performance method with Fraction collector, make sure
the Outlet valve fractionation tubing is connected to Fraction collector with at least
5 pre-weighed tubes.
•
Make sure that the system is pre-filled with DM water.
•
Make sure to take note of all the required observations by recording parameters
while the System performance method is in progress. Enter the observed values in
the System report template presented in Section 11.1 System Performance Report,
on page 278.
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5 Prepare the system for a run
5.4 System performance
5.4.2 System performance method from ÄKTA start
5.4.2
System performance method from ÄKTA start
Instruction
Follow the instructions below to initiate the System performance method from the Instrument Display.
Note:
112
Insert a USB memory stick to save the results.
Step
Action
1
From the ÄKTA start home screen, tap Method Run.
2
In the Method run screen, tap Prepare system.
3
Select System performance method. To initiate the method, tap Select.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.2 System performance method from ÄKTA start
Step
Action
4
Tick the check box in order to save the results on a USB memory stick, and
then tap Run to start the System Performance test.
Note:
Provide a unique file name.
Result: The Run view screen will be displayed.
Note:
• It is recommended not to edit any run parameters during a run to avoid
the failure of the test.
•
If required, the System performance method run can be ended before it
is completed, tap End to abort the test.
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5 Prepare the system for a run
5.4 System performance
5.4.2 System performance method from ÄKTA start
Step
Action
5
Tap Exit to close the screen when the System performance method is
completed.
Note:
Make sure not to unplug the USB memory stick until the system generates
the report (BMP file).
6
Review the report whether the test passed or failed, based on the Acceptance
criteria presented below.
Acceptance criteria
Time
(min)
Activity
Check
Approved interval
0
Pump wash
Wash valve position
Mobile phase out
through Waste
1
1 ml/min, 0% B, flow through
Outlet valve, Waste position
Back pressure
≤ 0.05 MPa
2
Repeat UV Auto zero
3
5 ml/min
Back pressure
0.06 to 0.2 MPa
UV level
± 10 mAU
Conductivity level
± 1 mS/cm
Max. UV level
300 to 380 mAU
Max. Conductivity
level
65 to 95 mS/cm
4
7
114
1 ml/min, Sample valve, Sample position
1 ml/min, Sample valve,
Buffer position
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.2 System performance method from ÄKTA start
Time
(min)
Activity
Check
Approved interval
10
Request switch Injection valve
to Inject position.
Max. UV level
300 to 380 mAU
Max. Conductivity
level
65 to 95 mS/cm
Weigh fraction no.
2, 3 and 4.
0.8 to 1.2 g
Max. diff. between
fractions
0.1 g
13
Request switch Injection valve
back to Load position.
15
Start gradient, 0 to 100% B in
10 minutes, start fractionation/collection.
19
End fractionation 1
20
End collection 2
Weigh beaker
4.2 to 5.8 g
25
End gradient, stay at 100% B
Gradient
Straight, no negative dips.
28
50% B
Gradient level 3
45 to 55% B
36
0 %B (Re equilibration)
41
End
Check all connections for leakage
No leakages.
1
With Fraction collector
2
Without Fraction collector
3
UV 50% B / UV 100% B
Note:
If the System performance method fails, analyze the cause for the failure
based on the acceptance criteria. Perform the following actions:
•
Re-calibrate the failed module
•
Use buffer with proper composition.
•
Clean the failed module or entire system. Refer Chapter 8 Maintenance,
on page 219 for more details on cleaning.
•
Carefully follow the test instructions.
•
Repeat the System performance method until it passes.
ÄKTA start Operating Instructions 29-0270-57 AF
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5 Prepare the system for a run
5.4 System performance
5.4.2 System performance method from ÄKTA start
116
•
If wavy or fluctuations in gradient is observed, then perform Switch valve
timing optimization.
•
If the test fails after following the above actions, replace the failed module.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.3 System performance method from UNICORN start
5.4.3
System performance method from UNICORN start
Instructions
Follow the instructions below to initiate the System performance method from UNICORN
start.
Step
Action
1
Start the test from UNICORN start System control:System:Performance
Test and Report.
2
Select method based on the Fraction collector configuration:
•
Performance method with Frac: when the Fraction collector is enabled.
•
Performance method without Frac: when the Fraction collector is
disabled.
3
Read the method notes before starting the run.
4
Make a note of the result file location.
5
Run the System performance method.
6
The test report states whether the System performance method passed or
failed.
Manually verify Pressure limits, Fractionation/collection volumes, gradient
levels and all connections for leakage during the test, using the Acceptance
criteria presented below.
Acceptance criteria
Time
(min)
Activity
Check
Approved interval
1
1 ml/min, 0% B, flow through
Outlet valve waste position
Back pressure
≤ 0.05 MPa
2
Repeat UV Auto zero
3
5 ml/min
Back pressure
0.06 to 0.2 MPa
15
Start gradient, 0 to 100% B in
10 minutes, start fractionation/collection.
ÄKTA start Operating Instructions 29-0270-57 AF
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5 Prepare the system for a run
5.4 System performance
5.4.3 System performance method from UNICORN start
Time
(min)
Activity
Check
Approved interval
19
End fractionation 1
Weigh fraction no.
2, 3 and 4.
0.8 to 1.2 g
Max. difference
between fractions
0.1 g
20
End collection 2
Weigh beaker
4.2 to 5.8 g
25
End gradient, stay at 100% B
Gradient 3
Straight, no negative dips.
41
End
Check all connections for leakage
No leakage.
1
With Fraction collector
2
Without Fraction collector
3
UV 50%B / UV 100%B
Note:
118
•
Make sure to update the Performance result text file with manually observed recordings and then print the report.
•
The other parameters are automatically checked and pass/fail reports are
generated in the report. For detailed list of acceptance criteria, refer to
Section 5.4.2 System performance method from ÄKTA start, on page 112.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.3 System performance method from UNICORN start
Illustration of the System
performance test
The illustration below represents a typical System performance method result file generated from UNICORN start.
Note:
It is recommended to optimize the timing of switch valve when wavy gradients
or fluctuations are observed. For detailed description, see Section 5.4.4 Switch
valve timing, on page 120.
Note:
If the System performance method fails, analyze the cause for the failure
based on the acceptance criteria. Perform the following actions:
•
Re-calibrate the failed module
•
Use buffer with proper composition.
•
Clean the failed module or entire system. Refer Chapter 8 Maintenance,
on page 219 for more details on cleaning.
•
Carefully follow the test instructions.
•
Repeat the System performance method until it passes.
•
If wavy or fluctuations in gradient is observed, then perform Switch valve
timing optimization.
•
If the test fails after following the above actions, replace the failed module.
ÄKTA start Operating Instructions 29-0270-57 AF
119
5 Prepare the system for a run
5.4 System performance
5.4.4 Switch valve timing
5.4.4
Switch valve timing
Introduction
Switch valve timing is used to optimize the switch valve (Buffer valve) timing of ÄKTA
start. It is recommended to optimize the timing of switch valve when wavy gradients
are obtained or when fluctuations in the step gradient are observed during either System
performance method or chromatography runs.
Note:
•
The default Switch valve timing A is 4 sec.
•
Switch valve timing B is 5 sec.
•
Switch valve time can be set between 3.0 and 5.0 sec with 0.1 sec increments using Advanced timing.
•
The gradient fluctuations or wavy gradients are highly dependent on the
flow rates. It is recommended to modify the switch valve timing if waviness/fluctuations are seen for specific flow rates.
•
After changing the Switch valve time, perform a System performance
method to evaluate the gradient fluctuations/wavy gradients. Alternatively
a manual run of 50% B for 10 min can also be performed to evaluate the
gradient fluctuations/wavy gradients.
Illustration of a typical operation
The illustration below shows the result of a System performance method where fluctuations were observed in the gradient (arrow).
120
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5 Prepare the system for a run
5.4 System performance
5.4.4 Switch valve timing
The Switch valve timing was changed to 5 sec, the test was repeated and the result
showed an acceptable gradient performance (arrow).
Note:
Alternatively a manual run of 50% B for 10 min can also be performed to
evaluate the gradient fluctuations/wavy gradients.
Set Switch valve timing
Follow the instructions below to change the Switch valve timing. The Switch valve
timing can be changed from the Instrument Display.
Step
Action
1
From the ÄKTA start home screen, tap Settings and service.
Result: The Settings and service screen 1 opens.
ÄKTA start Operating Instructions 29-0270-57 AF
121
5 Prepare the system for a run
5.4 System performance
5.4.4 Switch valve timing
Step
Action
2
Tap System.
Result: The System screen opens.
3
Tap Switch valve timing.
Result: The Switch valve timing screen opens.
4
Select Switch valve timing B to change the Switch valve timing to 5 sec.
5
Tap Save to save the changed timing.
6
If wavy gradients persist, or if fluctuations on step gradient levels are large,
select Advanced timing to set switch valve timing other than Switch valve
timing A and Switch valve timing B.
Note:
It is recommended to use the Advanced timing if the options for Switch valve
timing A or Switch valve timing B have not yielded satisfying results.
7
122
Set Switch valve time in the range of 3.0 to 5.0 sec.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.4 System performance
5.4.4 Switch valve timing
Step
Action
8
Tap Save to save the optimized timing.
9
Perform the System performance method or 50% gradient runs until satisfactory results are obtained.
ÄKTA start Operating Instructions 29-0270-57 AF
123
5 Prepare the system for a run
5.5 Connect a column
5.5
Connect a column
Introduction
This section describes how to connect a column to ÄKTA start. Different types of column
holders are available for the columns that are to be used with the instrument. See
Chapter 10 Reference information, on page 260 for selecting an appropriate column
holder.
The column is connected in the flow path between the Injection valve and UV Monitor,
as shown in the illustration of the flow path in Section 5.1 Flow path overview, on page 87.
Column placement
Depending on column dimension, choose the appropriate location on the instrument to
place the column. Column holder rails are located on the front and on the right side of
the instrument, as shown in the image below.
124
•
Front side of the instrument, for small columns (e.g., HiTrap™ columns)
•
Right side of the instrument, for large columns (e.g., column length 60 cm)
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.5 Connect a column
Connect a column
Follow the instructions below to connect a column to the instrument.
Step
Action
1
Attach an appropriate column holder to the column holder rail on the instrument.
2
Remove the column stoppers, mount the column on the union connector if
the column type requires a union.
ÄKTA start Operating Instructions 29-0270-57 AF
125
5 Prepare the system for a run
5.5 Connect a column
126
Step
Action
3
Fix the column to the column holder.
4
Connect the 0.75 mm i.d. PEEK tubing from the Injection valve port 1 to the
column head.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.5 Connect a column
Step
Action
5
Connect the 0.75 mm i.d. PEEK tubing from the bottom of the UV Monitor to
the bottom of the column.
Note:
The 0.75 mm i.d. PEEK tubing should not be disconnected from the UV Monitor
inlet when the column is removed. See Section 8.3.1 Disconnect the column,
on page 225.
Note:
Do not overtighten when connecting columns. Overtightening might break the
connectors or squeeze the tubing and thereby obstructing the flow.
ÄKTA start Operating Instructions 29-0270-57 AF
127
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6
Run Prepare system methods
Introduction
This section describes how to prepare the flow path and the column before starting a
chromatography run.
In this section
This section contains the following subsections:
Section
128
See page
5.6.1 Pump wash A
129
5.6.2 Pump wash B
131
5.6.3 Washout fractionation tubing
133
5.6.4 Column preparation
136
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.1 Pump wash A
5.6.1
Pump wash A
Introduction
The Pump wash A method is used before the start of a new run or in case of changing
the buffers. During Pump wash A the flow is directed through Wash valve to Waste.
Note:
•
Pump wash A is performed at 10 ml/min for 1 min through Buffer A port.
•
Pump wash A is important for preventing carryover and cross-contamination among Buffers/Samples.
•
It is recommended to first wash with DM water, and then wash with the
buffer of choice.
•
The Pump wash A method cannot be edited.
Requirements
Required solutions are:
•
DM water
•
Buffer solution
Instruction
Follow the instructions below to perform a Pump wash A run. The Pump wash A procedure is initiated from the Instrument Display.
Step
Action
1
Immerse the buffer A inlet tubing in DM water or buffer.
2
In the ÄKTA start home screen, tap Method run.
ÄKTA start Operating Instructions 29-0270-57 AF
129
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.1 Pump wash A
Step
Action
3
In the Method run screen, tap Prepare system.
4
Select Pump wash A and then tap Select to initiate the method.
Result: The following screen opens.
Note:
If required, the Pump wash A run can be ended before it is completed by
tapping End to stop the run.
5
130
When the run is completed, tap Exit to close the Pump wash A screen.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.2 Pump wash B
5.6.2
Pump wash B
Introduction
The Pump wash B method is used before the start of a new run or in case of changing
the buffers. During Pump wash B the flow is directed through Wash valve to Waste.
Note:
•
Pump wash B is performed at 10 ml/min for 1 min through Buffer B port.
•
Pump wash B is important for preventing carryover and cross-contamination among buffers and samples.
•
It is recommended to first wash with DM water, and then wash with the
buffer of choice.
•
The Pump wash B method cannot be edited.
Requirements
Required solutions are:
•
DM water
•
Buffer solution
Instruction
Follow the instructions below to perform a Pump wash B run. The Pump wash B procedure is initiated from the Instrument Display.
Step
Action
1
Immerse the buffer B inlet tubing in DM water or buffer.
2
In the ÄKTA start home screen, tap Method run.
ÄKTA start Operating Instructions 29-0270-57 AF
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5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.2 Pump wash B
Step
Action
3
In the Method run screen, tap Prepare system.
4
Select Pump wash B and then tap Select to initiate the method.
Result: The following screen opens.
If required, the Pump wash B run can be ended before it is completed by
tapping End to stop the wash in advance.
5
132
When the run is completed, tap Exit to close the Pump wash B screen.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.3 Washout fractionation tubing
5.6.3
Washout fractionation tubing
Introduction
The Washout fractionation tubing method is used to clean the fractionation tubing. It
is recommended while collecting the fractions without Fraction collector using Outlet
valve, and in between different runs using the Fraction collector.
Note:
Flow is diverted from fractionation tubing to collection tube, through Outlet
valve.
Requirements
Required cleaning solutions are:
•
DM water
•
Buffer solution
Instruction
Follow the instructions below to perform a Washout fractionation tubing run. The
Washout fractionation tubing procedure is initiated from the Instrument Display.
Step
Action
1
Immerse the buffer inlet tubing in DM water or buffer.
2
Remove the column from the flow path and re-connect the flow path. For
detailed instructions, see Section 8.3.1 Disconnect the column, on page 225.
3
Place the fractionation tubing in the waste container.
4
In the ÄKTA start home screen, tap Method run.
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5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.3 Washout fractionation tubing
Step
Action
5
In the Method run screen, tap Prepare system.
6
Select Washout fractionation tubing and then tap Select to initiate the
method.
7
Set the required run parameters:
•
Flow rate, flow rate (ml/min)
•
Pressure limit, pressure limit (MPa)
•
Run volume, run volume (ml)
Tap Run to initiate the method.
134
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.3 Washout fractionation tubing
Step
Action
Result: The following screen opens.
Note:
If required, the Washout fractionation tubing run can be ended before it is
completed by tapping End to stop the washout in advance.
8
When the run is completed, tap Exit to close the Washout fractionation
tubing screen.
ÄKTA start Operating Instructions 29-0270-57 AF
135
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.4 Column preparation
5.6.4
Column preparation
Introduction
The Column preparation method is used to prepare a new column or to equilibrate the
column. It is recommended to equilibrate columns before starting a new run.
Requirements
Required solution is:
•
Buffer solution
Instructions
Follow the instructions below to prepare the column for a run. The Column preparation
procedure is initiated from the Instrument Display.
136
Step
Action
1
Immerse the buffer inlet tubing in the intended buffer.
2
Connect the column into the flow path. For detailed instructions, see Section 5.5 Connect a column, on page 124.
3
In the ÄKTA start home screen, tap Method run.
4
In the Method run screen, tap Prepare system.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.6 Run Prepare system methods
5.6.4 Column preparation
Step
Action
5
Select Column preparation and then tap Select to initiate the method. For
detailed instructions, see Section 6.4.5 Prepare system methods, on page 192.
6
Set the required run parameters and then tap Run to initiate the method.
Result: The following screen opens.
Note:
If required, the Column preparation run can be ended before it is completed
by tapping End to stop the run.
7
When the run is completed, tap Exit to close the Column preparation screen.
ÄKTA start Operating Instructions 29-0270-57 AF
137
5 Prepare the system for a run
5.7 Sample application
5.7
Sample application
Sample application
The table below shows the different modes of sample application available for ÄKTA
start. The sample application technique can be selected from the Instrument display in
the Run parameters screen or from UNICORN start. For details, see Chapter 6 Operations
from the Instrument Display, on page 158 and UNICORN start 1.0 User Manual.
Sample volume
Sample application
Loop type
25 μl to 5 ml
via Loop
Sample loop
10 ml to 150 ml
via Loop
Superloop™, 10 ml
Superloop, 50 ml
Superloop, 150 ml
> 5 ml
Note:
138
via Pump, from the Sample
valve port I (Sample)
-
Make sure to load only the recommended volume of sample in the column to
obtain good results. For details, see the Column instructions.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.7 Sample application
Injection valve description
The Injection valve enables the application of a sample onto the column from a sample
loop connected to the valve. The illustration below shows the different positions of the
Injection valve. The Injection valve position can be changed manually by turning the
lever to the left (Load Sample position) or the the right (Inject to column position).
Valve position: Load Sample
Port connection
Function
6-1
Default route for the system flow path
3-2
Directs the liquid manually injected through port 3 to the
sample loop.
Note:
A sample loop or a Superloop is connected to the ports 2 and
5 of the Injection valve.
5-4
Directs the liquid from the sample loop, through port 4, to
the waste container.
Note:
The path indicated by light orange arrows in the illustration
above is used during the manual filling of the loop (Sample
or Superloop) through port 3.
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5 Prepare the system for a run
5.7 Sample application
Valve position: Inject to column
Port connection
Function
6-5
Diverts the system flow path to the sample loop.
2-1
Directs the liquid from the sample loop to the column so
that the sample loaded into the loop is transferred to the
column.
Connect a sample loop
Follow the instructions below to connect a sample loop to the Injection valve.
Step
Action
1
Connect the sample loop between ports 2 and 5 of the Injection valve.
2
Make sure that waste tubing is connected to port 4 of the Injection valve.
Connect a Superloop
Follow the instructions below to connect a Superloop to the Injection valve.
140
Step
Action
1
Attach an appropriate column holder to the column holder rail on the right
side edge of the instrument.
2
Make sure that the Superloop is filled with liquid according to the Superloop
instructions.
3
Attach the Superloop to the column holder.
4
Connect the tubing from the bottom of the Superloop to port 2 of the Injection valve.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.7 Sample application
Step
Action
5
Connect the tubing from the top of the Superloop to port 5 of the Injection
valve.
6
Make sure that a waste tubing is connected to port 4 of the Injection valve.
Prime the sample tubing using
the Pump
Follow the instructions below to prime the sample tubing using DM water/Buffer, before
loading the sample using the Pump.
Step
Action
1
Connect a 1 mm i.d. ETFE tubing to port I (Sample) of the Sample valve.
2
Immerse the other end of the sample inlet tubing in the DM water/Buffer
container.
3
From the Instrument Display, select Manual Run.
For more details on manual run, see Section 6.3 Perform a manual run, on
page 165.
4
Tap the forward arrow to access the run parameters on screen 2/2.
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5 Prepare the system for a run
5.7 Sample application
Step
Action
5
Toggle to set Sample valve position to Sample so that the flow is delivered
from the sample inlet.
6
Tap Run to start the run.
7
End the run manually once the priming with required volume of DM water/buffer has completed.
Load the sample using the Pump
The sample can be applied directly using the Pump through the Sample valve. The direct
sample application technique allows the application of sample volumes larger than 5
ml.
Follow the instructions below to apply the sample directly using the Pump.
Step
Action
1
Connect a 1 mm i.d. ETFE tubing to port I (Sample) of the Sample valve.
2
Immerse the other end of the sample inlet tubing in the sample container.
3
From the Instrument Display, select Method Run.
Choose Quick start or Templates. For details, see Section 6.4 Perform a
method run, on page 173.
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5 Prepare the system for a run
5.7 Sample application
Step
Action
4
In the Run parameters screen, select the sample application via the Pump.
Note:
• When the sample is applied via the Pump, the Injection valve has to be
manually set to position Load (default).
•
Make sure to wash the sample inlet tubing with buffer A before immersing
the tubing into the sample tube. Make sure to keep sufficient volume of
sample to avoid air entering the tubing.
•
Make sure that there are no trapped air bubbles in the tubing.
•
Pre-fill the sample tubing with sample before the start of the run to ensure
that the tubing is filled with sample.
5
In the Run parameters screen, set the sample volume and the other required
parameters. For details, see Operation Overview, on page 160.
6
Tap Run to start the run.
Prime the sample loop before
injecting sample
A sample loop allows the injection of small sample volumes onto the column. The sample
application via the loop is performed in two steps:
1
Loading the sample loop with sample.
2
Injecting the sample from the sample loop onto the column.
Follow the instructions below to prime the sample loop using DM water/Buffer, before
injecting the sample using the manual Injection valve.
Step
Action
1
Fill a syringe with DM water/buffer.
Note:
Make sure that the Injection valve is set to position Load Sample.
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5 Prepare the system for a run
5.7 Sample application
Step
Action
2
Connect the syringe to the Injection valve port 3.
3
Load the DM water/buffer into the sample loop.
4
Repeat steps 1 to 3 using at least 5 times the loop volume, before loading
the sample.
Load the sample into the sample
loop
Follow the instructions below to load the sample into the sample loop.
Note:
144
Make sure to flush the loop with DM water and buffer using at least 5 times
the loop volume before injecting the sample.
Step
Action
1
Fill a syringe with sample.
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5 Prepare the system for a run
5.7 Sample application
Step
Action
2
Connect the syringe to the Injection valve port 3.
Note:
Make sure that the Injection valve is set to position Load Sample, which allows to fill the sample loop from the fill port 3.
3
Carefully load the sample into the sample loop. To avoid sample loss due to
siphoning, leave the syringe in the port until the sample has been injected
onto the column during the run.
Tip:
It is recommended to overfill the loop to make sure that the loop is completely
filled. Excess of sample will leave the valve through port 4.
4
From the Instrument Display select:
Method run, then choose Templates. For details, see Section 6.4 Perform a
method run, on page 173
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5.7 Sample application
Step
Action
5
In the Run parameters screen, select the sample application via the Loop
and set all the required run parameters. For details, see Chapter 6 Operations
from the Instrument Display, on page 158.
Switch the Injection valve position to the Inject position, when the following
screen is shown on the Instrument Display.
Note:
Make sure that the Injection valve is in Load position before starting the run.
6
After manually switching position, acknowledge the message by tapping
Continue.
The sample will be injected onto the column when the Injection valve is
switched manually to position Inject to column during the run.
7
Switch the Injection valve position to the Load position, when the following
screen is shown on the Instrument Display.
8
After manually switching the position of the Injection valve, acknowledge
the message by tapping Continue.
Note:
146
For Binding techniques (AC/IEX) it is advisable to empty the loop with at least
3 times the loop volume to achieve good/high sample recovery. This is not
advised for non binding techniques (DS/GF) as there are sample volume limitations due to the size of the column used.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.7 Sample application
Load the sample from a
Superloop
Superloop allows the injection of larger volumes of sample (10 to 150 ml) onto the column.
Follow the instructions below to apply the sample using a Superloop.
Step
Action
1
Fill a large volume syringe with sample.
2
Connect the syringe to the Injection valve port 3 and carefully inject the
sample into the Superloop.
Note:
Make sure that the Injection valve is set to position Load sample, which allows to fill the capillary loop from the fill port 3.
3
From the Instrument Display select:
Method run, then choose Quick start methods or Templates. For details,
see Section 6.4 Perform a method run, on page 173.
4
In the Run parameters screen, select the sample application via the Loop
and set all the required run parameters. For details, see Operation Overview,
on page 160.
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5 Prepare the system for a run
5.7 Sample application
Step
Action
5
Switch the Injection valve position to the Inject position, when the following
screen is shown on the Instrument Display.
Note:
Make sure that the Injection valve is in Load position before starting the run.
6
After manually switching position, acknowledge the message by tapping
Continue.
7
Switch the Injection valve position to the Load position, when the following
screen is shown on the Instrument Display.
The sample will be injected onto the column when the Injection valve is
switched manually to the position Inject to column during the run.
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5.7 Sample application
Step
Action
8
After manually switching the position of the Injection valve, acknowledge
the message by tapping Continue.
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5 Prepare the system for a run
5.8 Prepare the Fraction collector
5.8
Prepare the Fraction collector
Prepare the Fraction collector
Fractions are collected in tubes using the Fraction collector. Follow the instructions below
to prepare the Fraction collector if the Fraction collector is going to be used during a
run.
The following types of tubes can be placed in the tube holder of the Bowl assembly:
•
Eppendorf™ tubes (1.5 ml or 2 ml)
•
5 ml tubes (12 × 75 mm)
•
Centrifuge tubes (10 to 12 ml)
•
Falcon™ tubes (15 ml)
NOTICE
The Fraction collector should be connected or disconnected from
the instrument only when ÄKTA start is switched off.
Note:
Make sure that the Fraction collector is properly installed. See Connect Frac30
to ÄKTA start, on page 81.
Step
Action
1
Insert a sufficient number of collection tubes in to the Bowl assembly.
Note:
All the tubes must be of the same length and diameter and there should be
no empty spaces in the sequence.
2
Connect a 0.75 mm i.d. PEEK tubing to the Outlet valve port III (Collection).
Note:
The tubing must be about 50 cm long to ensure proper placement of the
Fraction collector and free movement of the dispenser arm.
3
Loosen the nut of the tubing holder and insert the outlet tubing into the
tubing holder and tighten the nut.
Note:
The PEEK tubing should extend slightly (2 to 3 mm) out of the tubing holder.
Make sure that the extended length of the tubing is short enough to avoid
any collision with the test tubes during fractionation.
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5.8 Prepare the Fraction collector
Step
Action
4
Fit the tubing holder into the corresponding port on the Dispenser arm (i.e.,
the outer or inner port is chosen according to the type of collection tubes
inserted in the Bowl assembly).
5
Gently move the arm to the dispensing position.
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5 Prepare the system for a run
5.8 Prepare the Fraction collector
Set the delay volume
The delay volume represents the volume between the UV and the Fraction collector or
the outlet that is used. The delay volume settings are done to make sure that the fractions
collected during fractionation correspond to the fractions indicated in the chromatogram.
When the Fraction collector is enabled, the delay volume is collected in the first tube (T1)
and the elution volume is collected in the subsequent tubes. Without the Fraction collector
enabled, the delay volume is collected in the collection beaker (the total collected volume
in the collection beaker will be delay volume + elution volume).
As the delay volume is affected by the length and diameter of the tubing, it should be
set according to the tubing used. Follow the instructions below to set the delay volume.
Step
Action
1
In the ÄKTA start home screen, tap Settings and service to access the instrument modules.
Result: The Settings and service Screen 1 opens.
2
In the Settings and service screen, tap System to access the system options.
Result: The System screen opens.
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5.8 Prepare the Fraction collector
Step
Action
3
In the System screen, tap Delay volume setting to access the settings.
Result: The Delay volume settings screen opens.
4
Enter the internal diameter (ID) in the Tube ID field and length of the tubing
from the Outlet valve to Fraction collector in the Tube length fields, and
then tap Save.
Result: The total delay volume from UV to Fraction collector is displayed in
the Total delay volume field.
Note:
The delay volume from UV to Outlet valve is constant (0.27 ml) in all ÄKTA
start instruments if the recommended tubing lengths and i.d. are used.
Note:
Make sure to use recommended length and ID of the PEEK tubing from UV to
Outlet valve to avoid incorrect calculation of the delay volume.
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5 Prepare the system for a run
5.9 Cold room operations
5.9
Cold room operations
Introduction
When purifying biomolecules that are temperature sensitive, chromatography runs are
performed in a cold room.
Preparation
Follow the instructions below to prepare the instrument for a run in the cold room.
Step
Action
1
Place ÄKTA start in the cold room.
2
If a UNICORN start computer is connected to the instrument, leave the
computer outside the cold room.
3
Allow the instrument to stabilize at the temperature of the cold room.
4
Tighten all connections and pump DM water through the system to check
for leaks.
5
Tighten any leaking connector.
Starting a run
Before starting a run, make sure that the temperature of the buffers has reached the
temperature set in the cold room.
Note:
The measured temperature of the system is the temperature in the Conductivity flow cell, which can differ from the ambient temperature.
Removal from the cold room
Follow the instructions below to remove the instrument from the cold room.
154
Step
Action
1
Switch off the instrument and disconnect the power cable before moving
the instrument out of the cold room.
2
Loosen all connections to prevent them sticking when the system returns
to room temperature.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.9 Cold room operations
Step
Action
3
Allow the instrument to stabilize at room temperature for at least a few
hours.
4
Tighten all connections and pump DM water through the system to check
for leaks.
5
Tighten any leaking connector.
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5 Prepare the system for a run
5.10 Starting a run
5.10 Starting a run
Final checks
Before starting a run, make the checks recommended below to prevent that problems
are not encountered once the run has been started.
Buffer
•
Check that the buffer inlet tubings A and B are immersed in the correct bottles containing the buffers of interest.
•
Check that there is sufficient buffer available.
Waste outlet
•
Check that the outlet tubings leading to waste from the Wash valve, Injection valve,
and the Outlet valve are placed in the waste container.
•
Check that the waste container is not full and that there is provision for the volume
diverted to it during the run.
Fraction collector
•
If the fraction collector, Frac30 is going to be used during the run, check that the
fraction collector is prepared and filled with collection tubes and the Outlet valve
collection PEEK tubing is connected to the Fraction collector and also that the Fraction
collector is enabled.
Column
•
Check that the correct column has been connected and equilibrated (if equilibration
is not included in the method).
Sample
•
Make sure that the sample is ready to be loaded via Pump, Loop, or Superloop.
Pump
•
Make sure to place the pump tubing properly over the pump head. Make sure to
close the pump hood before starting the run.
Result storage
•
If the result of the run has to be saved when using without UNICORN start, make
sure that a USB memory stick is connected to the instrument.
UNICORN start
156
•
Check that ÄKTA start is connected to a PC with UNICORN start installed.
•
Make sure that the system connection is established before starting the run. For
details, refer to UNICORN start 1.0 User Manual.
ÄKTA start Operating Instructions 29-0270-57 AF
5 Prepare the system for a run
5.10 Starting a run
Start a run
A chromatography run is performed on ÄKTA start is by either using a Quick start method
or Template, or by running the system manually. A run can be started from the Instrument
Display or from UNICORN start by selecting one of the run options available with the instrument.
Detailed instructions for starting a run are presented in Chapter 6 Operations from the
Instrument Display, on page 158. For starting a run from UNICORN start, refer to UNICORN
start 1.0 User Manual.
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6 Operations from the Instrument Display
6
Operations from the Instrument
Display
About this chapter
This chapter describes how to operate the instrument, perform a run and the procedures
after a run.
In this chapter
This chapter contains the following sections:
Section
158
See page
6.1 Introduction
159
6.2 Fractionation
162
6.3 Perform a manual run
165
6.4 Perform a method run
173
6.5 Procedures after a run
195
6.6 Manage methods and files
198
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.1 Introduction
6.1
Introduction
Workflow
A typical workflow for ÄKTA start is presented below. All the operations in the workflow
can be controlled from the instrument display. A chromatographic run can be performed
by either using a Quick start method, Template, or by operating the system manually.
The options for starting a run from the instrument display are:
•
Method run
•
Manual run
Detailed instructions are presented in Section 6.3 Perform a manual run, on page 165 and
Section 6.4 Perform a method run, on page 173.
ÄKTA start home
Method run options:
Manual run:
Set run parameters
Create method options:
- Create
- Edit
- USB import
- Delete
Settings and service
options for the modules:
Save new method
Procedures required after a run, such as the cleaning of the column, the system flow
path, can also be performed manually or using methods available in the Prepare system
menu.
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6 Operations from the Instrument Display
6.1 Introduction
Calibration of the modules and service can be performed from the Settings and service
screen. Detailed instructions for calibration are presented in Section 5.3 Calibrations, on
page 91.
Method management operations such as create, edit, and import a method can be
performed from the Create method screen. For detailed instructions, see Section 6.6
Manage methods and files, on page 198.
Operation Overview
The ÄKTA start home screen displays four different options for the user to select and
perform operations from. Instructions for each operation are presented in separate
sections in this chapter. For a description of the options available in the main screen,
see Chapter 3 System description, on page 36.
Checklist
Make sure that the system is correctly prepared. Check that:
160
•
The system is prepared according to Chapter 5 Prepare the system for a run, on
page 86 and the modules are calibrated according to Section 5.3 Calibrations, on
page 91.
•
A suitable column has been selected for the application. Consider target protein,
pressure range, and optimal flow rate.
•
A suitable sample application technique will be used. See Section 5.7 Sample application, on page 138.
•
The buffer inlet tubing is immersed into correct buffer bottles. Consider the volume
required for the intended application.
•
The waste tubing is inserted into an appropriate waste container. Consider container
size and its material.
•
No tubing is twisted or blocked and the flow path is free from leakage.
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.1 Introduction
•
The Fraction collector configuration needs to be either enabled or disabled as required.
•
If the Fraction collector is used, make sure to use tubes with the same tube size.
•
The delay volume is set.
Specifications of run parameters
Parameter
Range
Increment
Flow rate
0.5 to 5.0 ml/min
0.1
Column volume (CV)
1 to 1000 ml, 0 to 150 ml
1 ml
Pressure limit
0.1 to 5 bar
0.1 bar
Sample volume
Pump: 0.1 to 1000.0 ml, 0.1 to 1000 ml
0.1 ml
Sample loop: 0.1 to 1000 ml
0.1 ml
Superloop: 0.1 to 1000 ml
0.1 ml
Wash volume
0.0 to 50.0 CV
0.1 CV
Equilibration volume
0.0 to 50.0 CV
0.1 CV
Elution volume
0.0 to 100 CV
0.1 CV
Target B concentration (%)
0 to 100%
1%
Gradient length
0.0 to 100.0
0.1
Fractionation volume
0.5 to 15 ml
0.1 ml
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6 Operations from the Instrument Display
6.2 Fractionation
6.2
Fractionation
Fractionation options
For many purification schemes it is important to collect fractions of the eluent. ÄKTA
start offers the fractionation options presented in the table below.
Instrument configuration
Fractionation options
ÄKTA start + UNICORN start + Frac30
•
Fixed volume fractionation
•
Peak fractionation
ÄKTA start + UNICORN start
•
-
Level based
-
Slope based
Single Peak collection
-
Level based
ÄKTA start + Frac30
•
Fixed volume fractionation
ÄKTA start
•
Collection of elution volume
Handling the delay volume
For setting the delay volume refer to Section 5.8 Prepare the Fraction collector, on page150.
Fractionation with Frac30
The delay volume is collected in the first tube (T1), followed by the rest of the fractions
in the subsequent tubes.
Fractionation without Frac30
The delay volume is collected in the collection container along with the fraction (i.e., Total
collected volume = delay volume + fraction volume).
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6 Operations from the Instrument Display
6.2 Fractionation
Fractionation using ÄKTA start
For detailed instructions on fractionation operations using UNICORN start, refer to
UNICORN start 1.0 User Manual.
ÄKTA start with Frac30
A representative chromatogram depicting fractionation using fixed volume fraction
collection in ÄKTA start with Frac30 is shown below.
Note:
Make sure to set fraction volumes that suit the column being used and use an
adequate number of collection tubes.
ÄKTA start using the Outlet valve (without Frac30)
A representative chromatogram depicting collection using the Outlet valve in ÄKTA start
(without Frac30) is shown below.
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6 Operations from the Instrument Display
6.2 Fractionation
Note:
164
•
The UV to Outlet valve volume (0.27 ml) is constant for all ÄKTA start instruments if the recommended tubing lengths and i.d. are used.
•
Make sure to use recommended length and ID of PEEK tubing from UV to
Outlet valve to avoid incorrect calculation of the delay volume.
•
Make sure to update the length and ID of the PEEK tubing (Settings and
service:System:Delay volume setting) in case the tubing is not of the
recommended length and ID.
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.3 Perform a manual run
6.3
Perform a manual run
Introduction
This section describes how to start a manual run by configuring the run parameters
from the instrument display and how to control an ongoing run.
In this section
This section contains the following subsections:
Section
See page
6.3.1 Manual run
166
6.3.2 Monitor and control the run
168
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165
6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.1 Manual run
6.3.1
Manual run
Start a run
Follow the instruction below to start a manual run.
Note:
Make sure that a USB memory stick is connected to the instrument. If the
instrument does not detect a USB memory stick, the result will not be saved.
Step
Action
1
In the ÄKTA start home screen, tap Manual run to access the run parameters
for a manual run.
Tap the forward arrows to access additional run parameters.
2
•
Set run parameters:
-
Flow rate, flow rate (ml/min)
-
Pressure limit, pressure limit (MPa)
-
Conc B, buffer B concentration (%)
Use up/down arrows to set the values, or use the numpad to type in the
values.
166
•
Tick the checkbox Save Result to USB if you want to save the result. The
result file name can be edited by setting the digits in the range 00 to 99.
•
Tap Run if no other parameters needs to be set.
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.1 Manual run
Step
Action
Note:
Make sure that the values for the Flow rate and Pressure limit are appropriate
for the chosen column. Refer to the column manual for details.
If the pressure reaches above the set limit, the instrument will enter the Pause
state.
3
•
•
Toggle to set which valve positions are open:
-
Sample valve: set as Buffer or Sample so that the flow is delivered
from either the buffer inlets or the sample inlet.
-
Wash valve, set as Column or Waste to direct the flow to either the
column or to waste.
-
Outlet valve, set as Collection or Waste to direct the flow to either
the Fraction collector or to waste.
Set the Fractionation volume, the volume of the fraction to be collected
when the Fraction collector is enabled.
Use up/down arrows to set the value, or use numpad to type in the value.
Note:
To collect fractions, make sure that the Fraction collector is enabled. If the
Fraction collector is enabled, place the required number of tubes of adequate
volume in the Bowl assembly.
If enabled, the Fraction collector will home to position 1 at the beginning of
every run.
•
Tap Run to start the run.
Result: The Run view screen will be displayed.
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6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.2 Monitor and control the run
6.3.2
Monitor and control the run
Overview
From the Run view screen, the user can monitor and control the ongoing run.
The following options are available:
Option
Description
Graph
Displays the run-time UV absorbance curve.
Edit run
Allows the user to edit the run parameters of the ongoing run.
Pause
Temporarily pauses the run by stopping the Pump, hence no
flow of liquid in the flow path.
End
Terminates the current run.
View the chromatogram
Follow the instruction below to view the chromatogram of the ongoing run.
Step
Action
1
In the Run view screen, tap the graph icon to view the chromatogram.
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6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.2 Monitor and control the run
Step
Action
2
The graph displays the UV curve. The Y axis displays the UV absorbance
(mAU) and X axis the time (min).
Tap the return arrow to return to the Run view screen.
Edit the run
Follow the instruction below to edit the run parameters of an ongoing run.
Step
Action
1
In the Run view screen, tap Edit run to access the run parameters of the
ongoing run.
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6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.2 Monitor and control the run
Step
Action
2
•
Edit the run parameters:
Conc B, buffer B concentration (%)
Flow rate, flow rate (ml/min)
Fractionation volume, fractionation volume (ml)
Toggle Fractionation between Start and Stop to start or stop the
fractionation.
Use up/down arrows to adjust the values, or use the numpad to type in
the values.
If no other parameters need to be set, tap Execute to implement the
changes. To ignore the changes, tap Cancel.
•
Tap the forward arrow to access additional run parameters to edit.
Note:
Before starting the run, set the status of the Fraction collector to enabled or
disabled as required.
3
•
170
Toggle as needed to set which valve positions are open:
-
Sample valve: set as Buffer or Sample so that the flow is delivered
from either the buffer inlets or the sample inlet
-
Wash valve: set as Column or Waste to direct the flow to either the
column or to waste
-
Outlet valve: set as Collection or Waste to direct the flow to either
the Fraction collector or to waste
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.3 Perform a manual run
6.3.2 Monitor and control the run
Step
Action
•
Tick the Autozero UV checkbox if a UV baseline to zero is required.
•
Tick the Set event mark checkbox if you need to set an event mark in
the chromatogram.
•
After you have set the run parameters, tap Execute to implement the
changes.
Pause the run
Follow the instruction below to pause an ongoing run.
Step
Action
1
In the Run view screen, tap Pause to temporarily pause the run by stopping
the Pump.
2
To continue the run, tap Continue.
Note:
The run is paused and there is no flow of liquid in the flow path as the Pump
is stopped.
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6.3.2 Monitor and control the run
End the run
Follow the instruction below to end an ongoing run.
Step
Action
1
In the Run view screen, tap End to terminate the run.
Result: A Message screen that requires to confirm the action opens.
2
Tap Yes to confirm that you want to terminate the run or tap No to cancel
the action and return to the Run view screen.
Note:
When ending a run before it is completed, the partial result is stored on the
USB memory stick.
The USB memory stick stores the result files which can be viewed using
UNICORN start. Also a BMP file is generated which can be viewed from any
PC. For details, refer to Section 6.6.3 BMP result file, on page 211.
Do not remove the USB memory stick until the system generates the report
(BMP file).
3
Tap Exit to close the Run ended screen.
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6.4
Perform a method run
Introduction
This section describes the types of methods that can be selected for a run.
In this section
This section contains the following subsections:
Section
See page
6.4.1 Select a method type
174
6.4.2 Quick start
176
6.4.3 Templates
182
6.4.4 User defined methods
190
6.4.5 Prepare system methods
192
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6.4.1 Select a method type
6.4.1
Select a method type
Method types
Four different method types can be selected to perform a method run. The different
method types are specified below.
Quick start: Allows the user to run methods like affinity, Ion exchange, Gel filtration and
Desalting with method parameters predefined.
Templates: Allows the user to edit and run the predefined methods Affinity, Ion exchange,
Gel filtration and Desalting.
User defined: Allows the user to run user created methods or USB imported methods.
Prepare system: Allows the user to perform system operations, such as Pump wash,
Column preparation, Cleaning and System performance test.
The Quick start methods and Templates available with ÄKTA start are briefly described
in Section 6.4.2 Quick start, on page 176 and Section 6.4.3 Templates, on page 182.
For a description of the Prepare system methods, see Section 6.4.5 Prepare system
methods, on page 192, and Section 8.3 Cleaning the system flow path, on page 224.
Method type
Option
Quick start
•
AC step 1 ml HiTrap
•
AC step 5 ml HiTrap
•
DS 5 ml HiTrap
•
DS 53 ml HiPrep™
•
IEX step 1 ml HiTrap
•
IEX step 5 ml HiTrap
•
IEX gradient 1 ml HiTrap
•
IEX gradient 5 ml HiTrap
•
GF 16/60 HiPrep
•
Affinity (AC)
•
Desalting/buffer exchange (DS)
•
Ion exchange (IEX)
•
Gel filtration (GF)
Templates
User defined
174
Methods created by the user, based on the
predefined templates.
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6.4.1 Select a method type
Method type
Option
Prepare system
•
Pump wash A
•
Pump Wash B
•
Washout fractionation tubing
•
Column preparation
•
System cleaning
•
System performance method
Select a method
Follow the instruction below to select a method.
Step
Action
1
In the ÄKTA start home screen, tap Method run to access the method types
available with the instrument.
2
Select one of the following methods:
•
Quick start
•
Templates
•
User defined
•
Prepare system
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6.4.2 Quick start
6.4.2
Quick start
Introduction
Quick start contains "ready to run" methods to purify most common proteins based on
Affinity, Ion exchange, Gel filtration and Desalting techniques. Run parameters like column
volume, flow rate, equilibration and elution mode, and volume are predefined in the
method. The user needs to enter only the sample volume. For detailed description of
each Quick start method, refer to ÄKTA start System Cue Card.
Note:
If required, the run parameters can be changed using the Edit run option
during an ongoing run.
Quick start techniques
The table below describes the various kinds of quick start techniques that a user can
choose, based on application requirements.
Method
Chromatography
Technique
Details
AC step 1 ml/5ml
HiTrap
Affinity
Chromatography
Bound proteins are eluted in a single step,
using a single elution buffer.
Commonly used for purification of tagged
proteins, for example Histidine-tagged
proteins.
DS 5 ml/53ml
HiTrap™
Desalting
Proteins are eluted in a single step, using
a single elution buffer.
IEX step 1 ml/5ml
HiTrap
Ion Exchange
Chromatography
Bound proteins are eluted in a single, step
using a single elution buffer.
IEX gradient 1
ml/5ml HiTrap
Ion Exchange
Chromatography
Bound proteins are eluted using two
buffers with linear increase in the concentration of buffer B, over a specified time
followed by a step of 100% B.
GF 16/60 HiPrep
Gel Filtration
Proteins are eluted in a single step, using
a single elution buffer.
Note:
176
It is recommended to use the appropriate column as indicated in the template
names. For example, use HiTrap 1 ml column when selecting AC/IEX step1 ml
HiTrap, or 5 ml column when selecting AC/IEX step 5ml HiTrap.
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6.4.2 Quick start
Start a run
Follow the instruction below to start a run based on a Quick start method.
Note:
Step
Make sure that a USB memory stick is connected to the instrument. If the
instrument does not detect a USB memory stick, the result will not be saved.
Action
1
In the Method run screen, tap Quick start to access the templates.
2
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6.4.2 Quick start
Step
Action
•
To select a Quick start method, tap a radio button
or
Tap the forward arrow to access additional Quick start methods.
•
To continue with the selected method, tap Select.
Note:
Make sure to load the recommended sample volume for the selected column.
If large Gel filtration columns are used, it is recommended to pre-equilibrate
the column before starting the run.
3
•
The mode of sample application is Pump (default).
Note:
Sample application using Pump is used for all Quick start methods to
automate sample loading or to have an unattended chromatographic
run.
Note:
Sample application via Loop is not applicable.
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6.4.2 Quick start
Step
Action
•
Enter the sample volume in the Sample volume field .
Use up/down arrows to set the values, or use numpad to type in the
values.
•
Tick the checkbox Save Result to USB if you want to save the result. The
result file name can be edited by setting the digits in the range 00 to 99.
•
Tap Run to start the run.
Result: The Run view will be displayed.
Note:
Other run parameters can be edited using the Edit run option available in the
Run view screen.
4
In the Run view screen, the following options are available to monitor and
control the ongoing run (for details, see Section 6.3.2 Monitor and control the
run, on page 168):
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6.4.2 Quick start
Step
Action
Graph, to view the chromatogram.
Edit run, to change any run parameters in the current run.
Hold, to temporarily hold the run, with current set flow rate, valve positions
and B concentration.
Pause, to pause the current run.
End, to end the run before it is completed.
Note:
The run begins with a default pump wash. Pump wash is performed at 10
ml/min for 1 min with 30 sec of buffer B wash followed by 30 sec of buffer A
wash.
Edit run is disabled when the pump wash is in progress. During pump wash
the flow is directed through Wash valve to Waste.
Note:
The USB memory stick stores the result files which can be viewed using
UNICORN start. Also a BMP file is generated which can be viewed from any
PC. For details, refer to Section 6.6.3 BMP result file, on page 211.
Do not remove the USB memory stick until the system generates the report
(BMP file).
Hold the run
Follow the instruction below to hold an ongoing run.
Step
Action
1
In the Run view, tap Hold to temporarily hold the run.
Note:
Not applicable for a manual run. Hold option is active only in a method run.
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6.4.2 Quick start
Step
Action
2
To resume the run, tap Resume.
Note:
During hold the run is temporarily interrupted, with current flow rate, gradient
and valve positions sustained.
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6.4.3 Templates
6.4.3
Templates
Introduction
ÄKTA start provides four method templates based on the most commonly used purification techniques. The templates are provided with default run parameters. The parameters
can also be changed to suit the run conditions. New methods can be created and saved
from these predefined templates in the Create method option.
This section describes how to start a run using Templates.
Predefined method templates
The user can create customized purification methods based on the templates available
on the instrument. The predefined templates available with ÄKTA start are described
below.
182
Method
Description
Affinity (AC)
Affinity Chromatography separates molecules
based on the reversible interaction between the
target protein and the specific ligand attached to
a chromatography matrix.
Ion exchange (IEX)
Ion Exchange Chromatography is based on the
reversible interaction between a charged protein
and an oppositely charged chromatography
medium.
Gel filtration (GF)
Gel filtration, also known as size-exclusion chromatography, is a chromatography technique that
separates molecules based on differences in the
molecular size.
Desalting/buffer exchange (DS)
Desalting is a gel filtration technique that allows
rapid group separation of high molecular weight
substances from low molecular weight substances. Small molecules like salt, free labels and
other impurities are efficiently separated from the
high molecular weight substances of interest.
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6.4.3 Templates
Affinity (AC) or Ion exchange (IE)
Follow the instruction below to start a run based on Affinity (AC) or Ion exchange (IEX).
Note:
Make sure that a USB memory stick is connected to the instrument. If the
instrument does not detect a USB memory stick, the result will not be saved.
Note:
Before starting the run, set the status of the Fraction collector to enabled or
disabled as required.
Step
Action
1
In the Method run screen, tap Templates to access the different templates.
2
•
Tap a radio button to select a template that suits your application.
•
To continue with the selected technique, tap Select.
3
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6.4.3 Templates
Step
Action
•
Set the run parameters:
-
Column volume, column volume (ml)
-
Flow rate, flow rate (ml/min)
-
Pressure limit, pessure limit (MPa)
•
Tick the checkbox Save Result to USB if you want to save the result. The
result file name can be edited by setting the digits in the range 00 to 99.
•
Tap the forward arrow to access additional run parameters.
Note:
Make sure that the values for the Column volume, Flow rate and Pressure
limit are appropriate for the chosen column. Refer to the column manual for
details.
If the pressure reaches above the set limit, the instrument will enter the Pause
state.
Note:
The USB memory stick stores the result files which can be viewed using
UNICORN start. Also a BMP file is generated which can be viewed from any
PC. For details, refer to Section 6.6.3 BMP result file, on page 211.
Do not remove the USB memory stick until the system generates the report
(BMP file).
4
•
184
Select the mode of sample injection in the Sample from field: to be applied via Pump or via Loop. For detailed instructions on sample application, refer to Section 5.7 Sample application, on page 138.
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6.4 Perform a method run
6.4.3 Templates
Step
Action
•
Set the run parameters:
-
Sample volume, the volume of sample to be loaded onto the column
-
Equilibration volume, the volume of buffer A required for for equilibrating the column.
-
Wash unbound volume, volume of buffer needed after the sample
application to wash off the unbound molecules
Note:
For AC/IEX methods when loading sample through loop it is advisable to
empty the loop with 3 times the loop volume to achieve good sample recovery.
5
•
Configure the run parameters for Elution Option set as Isocratic:
-
Conc B, concentration of buffer B to elute the bound protein
-
Elution volume, volume needed to elute the bound protein from the
column
-
Fractionation volume, volume of the fraction to be collected when
the Fraction collector is enabled
or
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6.4.3 Templates
Step
Action
•
Configure the run parameters for Elution Option set as Gradient (Bound
proteins are eluted with continuous change of buffer B composition to
increase eluent strength over specified time):
-
Target conc B, maximum buffer B concentration level to be set in
the gradient
-
Gradient volume, volume needed to elute the bound protein from
the column
-
Fractionation volume, volume of the fraction to be collected when
the Fraction collector is enabled.
•
Tap back arrow to view or edit run parameters.
•
Tap Run to start the run.
Result: The Run view screen will be displayed.
Note:
The run begins with a default pump wash. Pump wash is performed at 10
ml/min for 1 min with 30 sec of buffer B wash followed by 30 sec of buffer A
wash.
Edit run is disabled when the pump wash is in progress. During pump wash
the flow is directed through Wash valve to Waste.
Gel filtration, Desalting/Buffer
Exchange
Follow the instruction below to start a run based on Gel Filtration or Desalting template.
186
Note:
Make sure that a USB memory stick is connected to the instrument. If the
instrument does not detect a USB memory stick, the result will not be saved.
Note:
Before starting the run, set the status of the Fraction collector to enabled or
disabled as required.
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6.4 Perform a method run
6.4.3 Templates
Step
Action
1
In the Method run screen, tap Templates to access the predefined method
templates.
2
•
Tap a radio button to select a template that suits your application (e.g.,
Gel filtration).
•
To continue with the selected technique, tap Select.
3
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6.4.3 Templates
Step
Action
•
Set the run parameters:
-
Column volume, column volume (ml)
-
Flow rate, flow rate (ml/min)
-
Pressure limit, pressure limit (MPa)
Use up/down arrows to set the values, or use numpad to type in the
values.
•
Tick the checkbox Save Result to USB if you want to save the result. The
result file name can be edited by setting the digits in the range 00 to 99.
•
Tap the forward arrow to access additional run parameters.
Note:
Make sure that the values for the Flow rate and Pressure limit are appropriate
for the chosen column. Refer to the column manual for details.
If the pressure reaches above the set limit, the instrument will enter the Pause
state.
Note:
The USB memory stick stores the result files which can be viewed using
UNICORN start. Also a BMP file is generated which can be viewed from any
PC. For details, refer to Section 6.6.3 BMP result file, on page 211.
Do not remove the USB memory stick until the system generates the report
(BMP file).
4
188
•
Select the mode of sample injection in the Sample from field: to be applied via Pump or via Loop. For detailed instructions on sample application, refer to Section 5.7 Sample application, on page 138.
•
Set the run parameters:
-
Sample volume, the volume of sample to be loaded in to the column
-
Equilibration volume, the volume of buffer A required for for equilibrating the column.
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6.4.3 Templates
Step
Action
Note:
Washout unbound is not applicable for GF/DS methods.
Note:
Make sure to load the recommended sample volume for selected column.
If large GF columns are used, it is recommended to pre-equilibrate the column
before starting the run.
5
•
•
Set the run parameters:
-
Elution volume, volume of buffer needed to elute the protein from
the column
-
Fractionation volume, volume of fractions to be collected when the
Fraction collector is enabled.
Tap Run to start the run.
Result: The Run view screen will be displayed.
Note:
Conc B is not applicable for GF/DS as elution takes place only with a single
buffer (buffer A).
Note:
The run begins with a default pump wash. Pump wash is performed with
buffer A at 10 ml/min for 30 sec.
Edit run is disabled when the pump wash is in progress. During pump wash
the flow is directed through Wash valve to Waste.
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6.4.4 User defined methods
6.4.4
User defined methods
Start a run
Follow the instruction below to start a run based on a user created or USB imported
method.
Note:
Step
Make sure that a USB memory stick is connected to the instrument. If the
instrument does not detect a USB memory stick, the result will not be saved.
Action
1
In the Method run screen, tap User defined to access the user created
methods.
2
•
Tap a radio button to select a user method to run.
•
To continue with the selected user method, tap Select.
3
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6.4.4 User defined methods
Step
Action
Set the run parameters:
•
Select the mode of sample injection in the Sample from field: to be applied via Pump or via Loop. For detailed instructions on sample application, refer to Section 5.7 Sample application, on page 138.
•
Sample volume, the volume of sample to be loaded in to the column
•
Tick the checkbox Save Result to USB if you want to save the result. The
result file name can be edited by setting the digits in the range 00 to 99.
•
Tap Run to start the selected method.
Result: The Run view screen will be displayed.
4
In the Run view screen, the following options are available to monitor and
control the ongoing run (for details, see Section 6.3.2 Monitor and control the
run, on page 168):
Graph, to view the chromatogram.
Edit run, to change any run parameters in the current run.
Hold, to hold the current run.
Pause, to pause the current run.
End, to end the run before it is completed.
Note:
The USB memory stick stores the result files which can be viewed using
UNICORN start. Also a bmp file is generated which can be viewed from any
PC. For details, refer to Section 6.6.3 BMP result file, on page 211.
Do not remove the USB memory stick until the system generates the report
(.bmp file).
Note:
USB imported methods which were created in UNICORN start cannot be
edited in the instrument. Use UNICORN start to edit those methods.
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6.4.5 Prepare system methods
6.4.5
Prepare system methods
Introduction
Predefined methods for the preparation and the cleaning of the system are available
with ÄKTA start. Use the Prepare system methods to clean the entire system flow path
when needed, and to fill the system with storage solution when the instrument is not
going to be used for a period of time. For detailed instructions, see Section 8.3 Cleaning
the system flow path, on page 224.
The system methods available with ÄKTA start are listed below:
•
Pump wash A
•
Pump wash B
•
Washout fractionation tubing
•
Column preparation
•
System cleaning
•
System performance method
The Pump wash A/B, Washout fractionation tubing, and Column preparation methods
required for the system preparation are presented in detail in Section 5.6 Run Prepare
system methods, on page128. The System performance method is presented in Section 5.4
System performance, on page 109.
System cleaning
Follow the instruction below to perform a system cleaning run. For detailed instructions
on cleaning the system using the System cleaning template, see Section 8.3.2 System
cleaning, on page 226.
Step
Action
1
In the Method run screen, tap Prepare system to access the system methods.
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6.4 Perform a method run
6.4.5 Prepare system methods
Step
Action
2
In the Select system method screen,
•
Select System cleaning.
•
To continue with the System cleaning method, tap Select.
•
Perform the operations described on the display:
3
1
Disconnect the column.
2
Connect Injection valve to UV with suitable tubing and connectors.
3
Place the buffer tubings (A & B) in cleaning solution.
For detailed instructions, see Section 8.3.2 System cleaning, on page 226.
•
Tap Continue to start the System cleaning run.
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6.4.5 Prepare system methods
Step
Action
4
While the System cleaning run is in progress, wait for the run to complete.
Note:
If required, the System cleaning run can be ended before it completes by
tapping End.
5
Tap Exit to close the System cleaning screen when the System cleaning
run is completed.
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6.5 Procedures after a run
6.5
Procedures after a run
Introduction
This section briefly describes:
•
How to evaluate a recorded result
•
How to clean the instrument after a run.
•
How to prepare the system for storage if the instrument is not going to be used for
a period of time.
The instrument and the columns should be cleaned between the runs. This will prevent,
for example, carryover and cross-contamination among samples, protein precipitation,
and clogging of the column or flow path. For further details on cleaning and maintenance
procedures, see Chapter 8 Maintenance, on page 219.
Evaluate a run
After a chromatographic run, the result stored on a USB memory stick can be transferred
to UNICORN start where it can be viewed, and evaluated. The result holds a complete
record of the run, including method, system settings, chromatogram, and run log. The
result can be also viewed by using the BMP result file that is generated and stored on
the USB memory stick. For details, refer to Section 6.6.3 BMP result file, on page 211.
Detailed instructions for the transfer of the result are presented in Section 6.6 Manage
methods and files, on page 198.
Detailed instructions for the evaluation of a result are presented in Chapter 7 Operations
from UNICORN start, on page 213, and in the UNICORN start 1.0 User Manual.
Clean the system
After a run is completed, perform the following:
•
Remove the column from the flow path and re-connect the flow path. For detailed
instructions, see Section 8.3.1 Disconnect the column, on page 225.
•
Rinse the flow path with cleaning solution and/or DM water using either System
cleaning or the Pump wash methods, as required. For detailed instructions, see
Section 8.3 Cleaning the system flow path, on page 224.
•
If required, remove the tubes from the Fraction collector. If there is any spillage, clean
the Bowl assembly with DM water.
•
Clean all spills on the instrument and on the bench using a damp cloth.
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6.5 Procedures after a run
•
Empty the waste container.
Clean and store the column
After a run is completed, perform the following:
•
Disconnect the column from the flow path.
•
Clean the column off-line according to the column instructions.
If the column is not going to be used for a couple of days or longer, perform the following:
•
Fill the column with the storage solution recommended in the column data sheet.
•
Detach the column from the instrument and store it according to the column recommendation.
System storage
If the instrument is not going to be used for a period of time, fill the system and the inlets
with storage solution (DM water, or 20% ethanol). For detailed instructions, see Section 8.7
Storage of the instrument, on page 238.
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6.5 Procedures after a run
Power off the instrument
Switch off the instrument by turning the Power switch to the O position.
Note:
When the instrument is switched off or not in use, make sure to open the pump
hood and release the pump tubing from the pump head.
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6.6 Manage methods and files
6.6
Manage methods and files
Introduction
This section describes how to create, edit, import and delete methods on ÄKTA start.
For information about how to create a method using the UNICORN start, see Chapter 7
Operations from UNICORN start, on page 213 or the UNICORN start 1.0 User Manual.
In this section
This section contains the following subsections:
Section
198
See page
6.6.1 Create method
199
6.6.2 Handling the USB memory stick
208
6.6.3 BMP result file
211
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6.6 Manage methods and files
6.6.1 Create method
6.6.1
Create method
Create method menu
The Create method menu allows the user to create a new method, edit, import, and
delete a method from the ÄKTA start Instrument Display.
Follow the instructions below to access the Create method options.
Step
Action
1
In the ÄKTA start home screen, tap Create method.
2
In the Create method screen, the following options are available:
Create, to create a new method using a predefined template
Edit, to edit a method or change run parameters for user created methods
stored on instrument
USB Import, to import a method developed on UNICORN start to the instrument using a USB memory stick
Delete, to delete a method that is stored on the instrument
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6.6 Manage methods and files
6.6.1 Create method
Create a method
Follow the instruction below to create a method using a predefined template.
Step
Action
1
In the Create method screen, tap Create.
Result: The Select templates screen opens.
2
•
Tap a radio button to select a template.
•
Tap Create to create a method based on the selected technique.
For details on the templates available in ÄKTA start, refer to Section 6.4.3
Templates, on page 182.
3
•
200
Set the run parameters:
-
Column volume, column volume (ml)
-
Flow rate, flow rate (ml/min)
-
Pressure limit, pressure limit (MPa)
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6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
•
Select Save Method as field if you want to set a method name. The file
name can be edited by setting the digits in the range 00 to 99.
Note:
Provide a unique method name. The created method will be saved under
the User defined methods menu.
Note:
Make sure that the values for the Column volume, Flow rate and Pressure
limit are appropriate for the chosen column. Refer to the column manual for
details.
If the pressure reaches above the set limit, the instrument will enter the Pause
state.
4
•
Select the mode of sample injection in the Sample from field: to be applied via Pump or via Loop. For detailed instructions on sample application, refer to Section 5.7 Sample application, on page 138.
•
Set the run parameters:
-
Sample volume, the volume of sample to be loaded onto the column
-
Equilibration volume, the volume of buffer A required for for equilibrating the column
-
Wash unbound volume, volume of buffer needed after the sample
application to wash off the unbound molecules
Note:
The Washout unbound volume is applicable only for AC/IEX techniques.
ÄKTA start Operating Instructions 29-0270-57 AF
201
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
5
•
Configure the run parameters for Elution Option set as Isocratic:
-
Conc B, concentration of buffer B to elute the bound protein
-
Elution volume, volume needed to elute the bound protein from the
column
-
Fractionation volume, volume of the fraction to be collected when
the Fraction collector is enabled
or
•
•
Configure the run parameters for Elution Option set as Gradient (Bound
proteins are eluted with continuous change of buffer B composition to
increase eluent strength over specified time):
-
Target conc B, maximum buffer B concentration level to be set in
the gradient
-
Gradient volume, volume needed to elute the bound protein from
the column
-
Fractionation volume, volume of the fraction to be collected when
the Fraction collector is enabled.
Tap Save to save the new method.
Result: A Message screen that requires to confirm the action will be displayed.
202
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6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
6
Tap Yes to confirm the saving of the method or tap No to cancel the action
and return to set the run parameters.
Note:
The created method will be saved under the User defined methods menu.
The system can store up to 10 methods only. Delete existing methods if new
methods need to be stored.
Edit a method
Follow the instructions below to edit a user defined method.
Step
Action
1
In the Create method screen, tap Edit to access the methods.
Result: The Select method to edit screen opens.
ÄKTA start Operating Instructions 29-0270-57 AF
203
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
2
•
In the Select method to edit screen, tap a radio button to select a user
method.
•
Tap Edit to start editing the run parameters for the selected method.
3
204
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
•
Select Save Method as field if you want to set a method name. The file
name can be edited by setting the digits in the range 00 to 99.
Note:
Provide a unique method name, for example AC02, DS05. The created
method will be saved under the User defined methods menu.
USB imported methods which were created using UNICORN start cannot
be edited from the instrument. Use UNICORN start to edit those methods.
4
Tap Yes to confirm the saving of the method
or
Tap No to cancel the action and return to set the run parameters.
Import a method
Follow the instruction below to import a method stored on a USB memory stick.
Note:
Step
Make sure that the USB memory stick containing the user defined methods is
connected to the instrument. For details about exporting a method, see Section 6.6.2 Handling the USB memory stick, on page 208.
Action
1
In the Create method screen, tap USB Import to access the methods.
ÄKTA start Operating Instructions 29-0270-57 AF
205
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Step
Action
2
•
Tap a radio button to select a method.
•
Tap Import to import the method.
Result: A Message screen that requires to confirm the action will be displayed.
3
Tap Yes to confirm the import of the selected file
or
Tap No to cancel the action and return to the file list.
Note:
The imported methods will be saved under the User defined methods menu.
Only one method can be imported at a time. If multiple methods are to be
imported, repeat the steps described above.
Note:
If the system memory is full, delete existing methods before importing a new
method.
206
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.1 Create method
Delete a method
Follow the instruction below to delete a user method.
Step
Action
1
In the Create method screen, tap Delete to access the methods.
2
•
Tap a checkbox to select a method.
•
Tap Delete to delete the method.
Result: A Message screen that requires to confirm the action opens.
or
To cancel the action and return to the Create method screen, tap Cancel.
Note:
Multiple files can be deleted at the same time.
3
Tap Yes to confirm the deletion of the selected files or tap No to cancel the
action and return to the file list.
ÄKTA start Operating Instructions 29-0270-57 AF
207
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.2 Handling the USB memory stick
6.6.2
Handling the USB memory stick
Introduction
ÄKTA start provides the user with the option to store result data on a USB memory stick.
The USB memory stick is used to save a result, BMP file and also for transferring methods
between the instrument and UNICORN start. The USB memory stick is also used to generate a System error report.
Note:
•
The result files will be saved in the GE folder which is automatically created
by the instrument once the USB memory stick is plugged in.
•
At any given point of time only 10 results can be stored in the GE folder.
To save further results, transfer the result files to another folder, PC or rename the GE folder.
Store result on a USB memory
stick
Follow the instructions below to store results generated in ÄKTA start, on a USB memory
stick.
Step
Action
1
Connect a USB memory stick to the instrument, via the USB port.
2
Start a Manual run or Method run.
3
In the Run parameters screen, tick the Save results to USB check box in
order to save the generated results on the USB memory stick.
When the run is complete, the results are saved in a GE folder.
4
A BMP result file is also generated. Hence, the chromatogram can be viewed
without the use of UNICORN start.
5
Export the results to UNICORN start to view the chromatogram and evaluate.
Note:
Make sure that the USB memory stick is not removed during the run. For more
details, see Do's and Dont's while handling the USB memory stick, on page 208.
Do's and Dont's while handling
the USB memory stick
•
208
Make sure that the USB memory stick is inserted completely into the instrument.
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.2 Handling the USB memory stick
•
Max supported USB memory stick is 32 GB. Minimum 1 GB free space is required to
execute read/write operations.
•
Only FAT32 file system is supported and this needs to be taken into account while
formatting the memory stick.
•
Only unplug the USB memory stick when the instrument display is in the Home screen.
•
It is preferred to keep a minimum number of files on the memory stick. Once you
take a backup of the files, delete them from the memory stick and then save onto a
computer. Avoid keeping unnecessary files in the memory stick.
•
Avoid using folders named GE on the USB memory stick. However, you may use
folders named for example GE_ or GExyz.
•
Always take backup of complete GE folder from USB memory stick and do not
backup individual files. It is recommended to take backups whenever you have
completed important runs.
Result file import from USB stick
to UNICORN start.
Follow the instructions below to export a result file generated in ÄKTA start, and import
it into UNICORN start.
Step
Action
1
Open the Evaluation module in UNICORN start.
2
Select File:Import:Import ÄKTA start results from USB..., and then import
the result files to a desired location on the computer.
3
View, analyze, report or print the result file.
Method Export from UNICORN
start to USB memory stick
Follow the instructions below to export a method created in UNICORN start, to a USB
memory stick.
Step
Action
1
Create a method using the Method Editor module in UNICORN start.
2
Connect a USB memory stick to the computer.
ÄKTA start Operating Instructions 29-0270-57 AF
209
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.2 Handling the USB memory stick
Step
Action
3
Select File:Export:Export Method... in order to export the created method
to a USB memory stick, connected to the computer.
Note:
Make sure that the method is stored in a GE folder.
Method Import to ÄKTA start –
USB import
Follow the instructions below to import a method from UNICORN start, to ÄKTA start.
Step
Action
1
Create a method using the Method Editor module in UNICORN start.
2
Select File:Export:Export Method for ÄKTA start to USB... in order to export
the created method to a USB memory stick.
3
Connect the USB memory stick to ÄKTA start.
Note:
Make sure that the method is stored in a GE folder.
210
4
From the ÄKTA start Home screen, tap Create method : USB import.
5
Select the method to import.
ÄKTA start Operating Instructions 29-0270-57 AF
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.3 BMP result file
6.6.3
BMP result file
Introduction
In order to provide the user with the option to view the result image using another type
of software outside of ÄKTA start and UNICORN start, the instrument provides the feature
to export the result in BMP format. This format facilitates viewing of the generated result
without UNICORN start.
Feature of exported result
•
The result file in BMP format can be opened and viewed using Operating Systems
(OS) such as Microsoft® Windows® operating system and Mac OS™.
•
The BMP result is saved and exported, if user selects the option to Save result to
USB before the start of a run.
•
The result file offers the user UV curve data with fractionation marks.
•
The result file contains 4 hours of run data.
•
The BMP file displays the necessary legends like Product name, run details, UV, Frac
marks.
•
In case of shutdown or power failure, the partial result will not be available. However,
forcible ending of the run by the User results in the export of result as BMP.
Generate a BMP file
Step
Action
1
Connect a USB memory stick to the instrument.
2
Start a Method run or Manual run.
ÄKTA start Operating Instructions 29-0270-57 AF
211
6 Operations from the Instrument Display
6.6 Manage methods and files
6.6.3 BMP result file
Step
Action
3
Tick the Save results to USB check box in order to save the results to the
USB memory stick.
Note:
A BMP file is only generated when the result is saved.
4
When the run is completed, the result will be saved and a BMP file will be
generated.
Note:
Do not unplug the USB memory stick when the BMP file generation is in
progress.
5
212
Transfer and connect the USB memory stick to a computer or else the image
file will not be generated. Open the generated BMP file, view or print the result.
ÄKTA start Operating Instructions 29-0270-57 AF
7 Operations from UNICORN start
7
Operations from UNICORN start
About this chapter
This chapter gives a brief insight into the four modules of UNICORN start: System Control
Method Editor, Evaluation and Administration.
In this chapter
This chapter contains the following sections:
Section
See page
7.1 System Control
214
7.2 Method Editor
216
7.3 Evaluation
217
7.4 Administration
218
Introduction
UNICORN start brings the following capabilities:
•
Simple and flexible method creation.
•
Easy system control using process picture and real time monitoring of manual and
method runs.
•
Ability to evaluate and compare results.
•
Create and print PDF reports.
•
Ability to manage (store, archive\retrieve, backup\restore) results generated from
ÄKTA start.
For detailed instructions on operations from UNICORN start, refer to the UNICORN start
1.0 User Manual.
ÄKTA start Operating Instructions 29-0270-57 AF
213
7 Operations from UNICORN start
7.1 System Control
7.1
System Control
Introduction
The System Control module is used to start, view, and control a run.
Illustration of the System Control
user interface
Main features
The main features of the System Control module are listed below:
214
•
A flow scheme representing the real time flow path with indications of the different
modules in wet side of the instrument. Current run status of the system is displayed.
•
The ability to control the instrument via simple clicks on the flow path, for example,
to turn the valves, set flow rates, change B concentrations and start/stop fractionation.
ÄKTA start Operating Instructions 29-0270-57 AF
7 Operations from UNICORN start
7.1 System Control
•
A real time chromatogram depicting the complete run with curves including UV,
conductivity, system flow, gradient concentrations, fraction marks, run logs and
pressure.
•
The ability to perform manual and method runs.
•
Ability to run predefined methods like Quick start, Templates and Prepare system
methods.
•
Ability to perform System performance method.
•
Ability to generate a System error report.
Note:
When the Fraction collector is enabled, the process picture will display a
Fraction collector image. If the Fraction collector is disabled, a collection beaker
image is displayed.
ÄKTA start Operating Instructions 29-0270-57 AF
215
7 Operations from UNICORN start
7.2 Method Editor
7.2
Method Editor
Introduction
The Method Editor module provides flexibility to create or edit the chromatography
methods.
Illustration of the Method Editor
module
The user interface of Method Editor is illustrated below.
Main features
The main features of the Method Editor module are listed below:
216
•
Ability to create methods from predefined templates like Affinity, Ion Exchange,
Desalting and Gel Filtration.
•
Flexibility to create customized methods by dragging and dropping chromatography
phases such as Prime and Equilibration, Sample Application, Wash Out Unbound,
Elution and Fractionation.
•
The methods created from Method Editor can either be run directly from System
Control or exported to a USB memory stick to run them from ÄKTA start directly.
ÄKTA start Operating Instructions 29-0270-57 AF
7 Operations from UNICORN start
7.3 Evaluation
7.3
Evaluation
Introduction
The Evaluation module is used to manage and evaluate the results from chromatography
runs.
Illustration of the Evaluation
module
The user interface of Evaluation is illustrated below.
Main features
The main features of the Evaluation module are listed below:
•
Open and view existing chromatogram results.
•
Compare two curves or chromatograms.
•
Perform peak integration analysis.
•
Create and print PDF reports.
•
Import results from ÄKTA start via a USB memory stick.
ÄKTA start Operating Instructions 29-0270-57 AF
217
7 Operations from UNICORN start
7.4 Administration
7.4
Administration
Introduction
The Administration module is used to manage the UNICORN start database and to review
UNICORN start and system logs.
Illustration of the Administration
module
Main features
The main features of the Administration module are listed below:
218
•
Ability to back up and restore operations, and archive and retrieve operations.
•
Review the UNICORN start and system logs.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8
Maintenance
About this chapter
This chapter describes the maintenance program for ÄKTA start and provides instructions
for routine maintenance.
In this chapter
This chapter contains the following sections:
Section
See page
8.1 Regular maintenance
220
8.2 Cleaning before planned maintenance/service
223
8.3 Cleaning the system flow path
224
8.4 Cleaning the UV flow cell
229
8.5 Cleaning the Conductivity flow cell
231
8.6 Other cleaning procedures
232
8.7 Storage of the instrument
238
8.8 Replacement of tubing and filters
240
ÄKTA start Operating Instructions 29-0270-57 AF
219
8 Maintenance
8.1 Regular maintenance
8.1
Regular maintenance
Introduction
Regular maintenance is important for safe and trouble-free operation of ÄKTA start. The
user should perform preventive maintenance on a daily, weekly and monthly basis.
Safety precautions
To avoid damage to person when performing maintenance on the instrument, follow
the instructions below.
WARNING
Electrical shock hazard. Do not open any covers or replace parts
unless specified in the user documentation. All other repairs should
be done by service personnel authorized by GE.
WARNING
Disconnect power. Always switch off power to the instrument before replacing any component on the instrument, unless stated
otherwise in the user documentation.
WARNING
Spillage Hazard. Avoid spillage of fluids on the surfaces of the instrument which have cables, plugs and other wirings. Be careful if
there is spillage of fluids on the tray while trying to remove the tray
from ÄKTA start.
CAUTION
Always use appropriate personal protective equipment during operation and maintenance of ÄKTA start.
220
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.1 Regular maintenance
Periodic maintenance schedule
The periodic maintenance schedule provides a guide to the user to ensure proper routine
maintenance of ÄKTA start.
Interval
Maintenance action
See section
Daily
Visually inspect the instrument for leakages in
the flow path.
-
Check the Pump for leakage. If there are signs
of liquid leaking from the Pump, check the integrity of the pump tubing and the tubing connections.
Note:
Make sure that the pump tubing is not left inside
the Pump when it is not in use.
Clean the column and the system flow path after use and leave the system filled with DM
water.
Section 6.5 Procedures
after a run, on
page 195
Note:
Section 8.3 Cleaning
the system flow path,
on page 224
If the instrument is not going to be used for a
few days, prepare the system for storage.
Weekly,
or when
required
Calibrate the Pump.
Section 5.3 Calibrations, on page 91
Visually inspect the inlet filters and clean them
if necessary.
Section 8.6.1 Cleaning
the inlet filters, on
page 233
Monthly,
or when
required
Clean the system flow path with 1 M NaOH and
rinse with DM water.
Section 8.3.2 System
cleaning, on page 226
Note:
Cleaning may be necessary more or less frequently, depending on the system usage and the
nature of the samples.
Yearly, or
when requried
Visually inspect the drive sleeve on the Fraction
collector. Replace if worn out.
ÄKTA start Operating Instructions 29-0270-57 AF
Maintenance Manual,
Chapter 7 - Disassembly
221
8 Maintenance
8.1 Regular maintenance
Other required maintenance
The following maintenance should be performed by the user of ÄKTA start when required.
222
Maintenance action
See section
Clean the instrument externally
Section 8.6.2 Cleaning the instrument externally,
on page 234
Clean the Fraction collector
Section 8.6.3 Cleaning the Fraction collector, on
page 235
Perform System cleaning
Section 8.3.2 System cleaning, on page 226
Clean the UV flow cell
Section 8.4 Cleaning the UV flow cell, on page 229
Clean the Conductivity cell
Section 8.5 Cleaning the Conductivity flow cell,
on page 231
Calibrate the touch screen
Section 5.3.2 Instrument Display calibration, on
page 93
Calibrate the UV flow cell
Section 5.3.5 UV Monitor calibration, on page 99
Calibrate the Conductivity cell
Section 5.3.6 Conductivity Monitor calibration, on
page 103
Pressure sensor zero offset
Section 5.3.3 Pressure sensor zero offset, on
page 95
Replace the inlet filters
Section 8.8.1 Replace the inlet filters, on page 241
Replace the tubing and connectors
Section 8.8.2 Replace the tubing and connectors,
on page 242
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.2 Cleaning before planned maintenance/service
8.2
Cleaning before planned maintenance/service
Cleaning before planned
maintenance/service
To ensure the protection and safety of service personnel, all equipment and work areas
must be clean and free of any hazardous contaminants before a Service Engineer starts
maintenance work.
Please complete the checklist in the On Site Service Health & Safety Declaration Form, or
the Health & Safety Declaration Form for Product Return or Servicing, depending on
whether the instrument is going to be serviced on site or returned for service, respectively.
Copy the form you need from Section 10.4 Health and Safety Declaration Form, on page271
or print it from the PDF file available on the User Documentation CD.
ÄKTA start Operating Instructions 29-0270-57 AF
223
8 Maintenance
8.3 Cleaning the system flow path
8.3
Cleaning the system flow path
Introduction
Cleaning of the system flow path is performed to prevent carryover between runs, contamination in the flow path and as a routine maintenance protocol.
Cleaning the system flow path is usually performed by using System cleaning or Pump
wash methods.
Note:
Before cleaning the system flow path, remove the column from the flow path.
For detailed instructions, see Section 8.3.1 Disconnect the column, on page 225.
WARNING
Hazardous biological agents during run. When using hazardous
biological agents, run the System cleaning method to flush the
entire system tubing with 1M NaOH and subsequently with distilled
water, before service and maintenance. NaOH can cause health
injurious, avoid spillage.
CAUTION
Hazardous substances. When using hazardous chemical and biological agents, take all suitable protective measures, such as
wearing protective glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe operation,
maintenance and decommissioning of the equipment.
Tip:
If hazardous chemicals are used for system or column cleaning, wash the
system or columns with a neutral solution in the last phase or step.
In this section
This section contains the following subsections:
Section
224
See page
8.3.1 Disconnect the column
225
8.3.2 System cleaning
226
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.3 Cleaning the system flow path
8.3.1 Disconnect the column
8.3.1
Disconnect the column
Introduction
The column should be removed from the flow path before cleaning the system flow path.
The flow path has to be re-connected between the manual Injection valve port 1 and
the UV inlet.
For column cleaning procedures and storage instructions, refer to the column catalogue.
Instruction
Follow the instructions below to remove the column and re-connect the flow path.
Step
Action
1
Disconnect the tubing from the column, as indicated by the arrows in the
illustration below (1).
2
Re-connect the flow path between the Injection valve and the UV Monitor,
as indicated by the arrows in the illustration below (2). Join the tubing by
using the union mounted on the tubing connected to the UV flow cell.
ÄKTA start Operating Instructions 29-0270-57 AF
225
8 Maintenance
8.3 Cleaning the system flow path
8.3.2 System cleaning
8.3.2
System cleaning
Introduction
The System cleaning method is used to clean the instrument flow path. System cleaning
is recommended to be performed to prevent carryover between runs, contamination in
the flow path, as a routine maintenance protocol and to prepare system for storage.
Note:
•
Cleaning is important for preventing cross-contamination and bacterial
growth in the instrument.
•
Prepare cleaning solutions of recommended concentration to ensure
proper cleaning.
•
It is recommended not to end the run before completion.
•
It is recommended to clean the inlets and outlets (sample tubing, fractionation tubing) from the Edit run screen.
Required solutions
The following cleaning solutions are required:
•
1 M NaOH
•
DM water
Instruction
Follow the instructions below to clean the system flow path. The System cleaning procedure is initiated from the Instrument Display.
226
Step
Action
1
Remove the column from the flow path and re-connect the tubing.
2
Immerse both the buffer inlets in 1M NaOH.
3
In the ÄKTA start home screen, tap Method run.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.3 Cleaning the system flow path
8.3.2 System cleaning
Step
Action
4
In the Method run screen, tap Prepare system.
5
Select System cleaning and then tap Select to initiate the method. For detailed instructions, see Section 6.4.5 Prepare system methods, on page 192.
6
•
•
Perform the operations presented on the display:
1
Disconnect the column.
2
Connect Injection valve to UV with suitable tubing and connectors.
3
Place the buffer tubings (A & B) in cleaning solution.
Tap Continue to start System cleaning.
ÄKTA start Operating Instructions 29-0270-57 AF
227
8 Maintenance
8.3 Cleaning the system flow path
8.3.2 System cleaning
Step
Action
7
When the run is completed, tap Exit to close the System cleaning screen.
Note:
228
•
Do not leave NaOH in the instrument in order to avoid damage of the
system. Wash the complete flow path with water.
•
Check the pH after a wash with water to make sure a complete removal
of NaOH.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.4 Cleaning the UV flow cell
8.4
Cleaning the UV flow cell
Maintenance interval
Clean the UV flow cell every six months, or when required. A clean flow cell is essential
for the acceptable performance of the UV Monitor.
NOTICE
Keep UV flow cell clean. Do not allow solutions containing dissolved
salts, proteins or other solid solutes to dry out in the UV flow cell.
Do not allow particles to enter the UV flow cell, as damage to the
flow cell may occur.
Required solutions
The following solutions are required:
•
Cleaning solution: 10% surfactant detergent solution, such as Decon™ 90, Deconex™
11, RBS 25, 1M HCl or 1M NaOH.
•
DM water
Note:
•
It is recommended to use 10% surfactant detergent solution for cleaning
the UV flow cell.
•
Heat the 10% surfactant detergent solution to 40°C to increase the
cleaning efficiency.
•
If NaOH is used, perform cleaning at 1ml/min and reduce the hold time to
5 min in step 3 of the method mentioned below.
•
NaOH should not be left in the flow cell for more than 20 minutes and
proper care should be taken to remove the NaOH completely from the flow
cell.
Cleaning the UV flow cell in-place
Follow the instructions below to clean the UV flow cell.
Note:
Before cleaning the UV flow cell, remove the column from the flow path and
re-connect the flow path. See Section 8.3.1 Disconnect the column, on page225.
Step
Action
1
Immerse the inlet tubing in cleaning solution.
ÄKTA start Operating Instructions 29-0270-57 AF
229
8 Maintenance
8.4 Cleaning the UV flow cell
230
Step
Action
2
Start a manual run and pump cleaning solution at 5 ml/min through the UV
flow cell for 10 minutes and pause the run.
3
Leave the UV flow cell filled with cleaning solution for 15 minutes.
4
Immerse the inlet tubing in DM water.
5
Resume the run and rinse the flow cell thoroughly with DM water.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.5 Cleaning the Conductivity flow cell
8.5
Cleaning the Conductivity flow cell
Maintenance interval
Clean the Conductivity flow cell when the Conductivity Monitor shows a slow response
or when the conductivity measurements are not comparable to any of the previous results.
Required solutions
The following solutions are required:
•
1 M NaOH
•
DM water
Cleaning the Conductivity flow
cell in-place
Follow the instructions below to clean the Conductivity flow cell.
Note:
Before cleaning the Conductivity flow cell, remove the column from the flow
path and re-connect the flow path. See Section 8.3.1 Disconnect the column,
on page 225.
Step
Action
1
Immerse the inlet tubing (either A or B) in 1M NaOH.
2
Start a manual run and pump 1M NaOH at 1 ml/min through the flow cell
for 10 minutes.
3
Pause the run. Leave the Conductivity flow cell filled with 1M NaOH for 15
minutes.
4
Immerse the inlet tubing in DM water.
5
Resume the run and rinse the flow cell thoroughly.
Note:
• Do not leave NaOH for a very long time in flow cell to avoid damage. Rinse
the flow path with water thoroughly.
•
Make sure that the NaOH is completely removed. The conductivity reading
in the Run view screen should be < 1 mS/cm.
ÄKTA start Operating Instructions 29-0270-57 AF
231
8 Maintenance
8.6 Other cleaning procedures
8.6
Other cleaning procedures
Introduction
This section provides instructions for additional cleaning procedures to be performed
by the user of ÄKTA start.
In this section
This section contains the following subsections:
Section
232
See page
8.6.1 Cleaning the inlet filters
233
8.6.2 Cleaning the instrument externally
234
8.6.3 Cleaning the Fraction collector
235
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.6 Other cleaning procedures
8.6.1 Cleaning the inlet filters
8.6.1
Cleaning the inlet filters
Maintenance interval
Clean the inlet filters when required, for example when the visual inspection shows that
the filters are clogged.
Required solutions
The following solutions are required:
•
1M NaOH
•
DM water
Instruction
Follow the instructions below to clean the inlet filters.
Step
Action
1
Pull off the support net and the inlet filter from the inlet filter holder. See
Section 8.8.1 Replace the inlet filters, on page 241.
2
Immerse and leave the inlet filter and the support net in 1 M NaOH for about
2 hours.
3
Remove the inlet filter and the support net from the NaOH solution and rinse
thoroughly with DM water.
4
Fit the inlet filter into the support net, and press it into position on the inlet
filter holder.
ÄKTA start Operating Instructions 29-0270-57 AF
233
8 Maintenance
8.6 Other cleaning procedures
8.6.2 Cleaning the instrument externally
8.6.2
Cleaning the instrument externally
Maintenance interval
Clean the instrument externally when required. Do not allow spilled liquid to dry on the
instrument.
Required material
The following materials are required:
•
Cleaning cloth
•
Mild cleaning agent or 20% ethanol
Instruction
Follow the instructions below to clean the instrument externally.
234
Step
Action
1
Check that no run is in progress.
2
Switch off the instrument.
3
Wipe the surface with a damp cloth. Wipe off stains using a mild cleaning
agent or 20% aqueous ethanol.
4
Let the instrument dry completely before using it.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.6 Other cleaning procedures
8.6.3 Cleaning the Fraction collector
8.6.3
Cleaning the Fraction collector
Maintenance interval
Clean the fraction collector when required, for example in case of liquid spill in the Bowl
assembly.
Required material
The following materials are required for cleaning the Bowl assembly:
•
Water
•
20% ethanol
•
Cleaning cloth
Instruction
Follow the instructions below to disassemble and clean the Bowl assembly.
Step
Action
1
Check that no run is in progress.
2
Switch off ÄKTA start and disconnect the Frac30 Cable.
3
Remove the collection tubes and disassemble the Bowl assembly from the
Base unit:
•
Gently move the Dispenser arm counterclockwise to the end position (1)
•
Push the drive assembly and hold it at the retracted position (2)
•
Lift and remove the Bowl assembly (3)
ÄKTA start Operating Instructions 29-0270-57 AF
235
8 Maintenance
8.6 Other cleaning procedures
8.6.3 Cleaning the Fraction collector
Step
Action
1
3
2
4
Wash the Bowl under a water tap. Use a mild detergent if required, and rinse
thoroughly with water.
5
Wipe the surface with a damp cloth. Wipe off stains using water.
6
Let the Bowl assembly dry completely before re-assembling.
7
Re-assemble the Bowl assembly on to the Base unit:
•
Orient the Bowl to match the aligning groove and the aligning features
on the Bowl holder (1)
•
Lower the bowl assembly on to the Base unit (2) and push the drive assembly to allow the Bowl assembly to get in position (3)
1
2
3
236
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.6 Other cleaning procedures
8.6.3 Cleaning the Fraction collector
Removing the tube holder
Note:
The tube holder is not intended to be open by the user. If required, the tube
holder may need to be removed from the Bowl assembly for cleaning.
Follow the instructions below to remove and then place back the tube holder in the Bowl
assembly.
Step
Action
1
To remove the test tube holder, unsnap the snap locks one by one and sequentially for ease of removal.
2
To fit the test tube holder on the Bowl base:
•
Align the holder with the help of the aligning groove.
•
Snap fit the part by operating one snap at a time in a continuous sequence by pressing at the edge of the part.
ÄKTA start Operating Instructions 29-0270-57 AF
237
8 Maintenance
8.7 Storage of the instrument
8.7
Storage of the instrument
Required material
The following materials are required:
•
DM water
•
20% ethanol
•
0.75 mm i.d. PEEK tubing
•
Waste container
Short term storage
If the system is not going to be used for a few days, follow the instructions below to
prepare the system for short term storage.
Note:
Before cleaning the flow path, remove the column and re-connect the flow
path. See Section 8.3.1 Disconnect the column, on page 225.
Step
Action
1
Immerse the buffer and the sample inlet tubing in DM water.
2
In the ÄKTA start home screen, tap Manual run. For detailed instructions,
see Section 6.3 Perform a manual run, on page 165.
Set the run parameters according to the table below.
3
Parameter
Setting
Flow rate
1 ml/min
Pressure limit
0.5 MPa
Select Buffer/Sample
Buffer
Wash valve
Column
Outlet valve
Waste
Tap Run to start the manual run.
Pump 20 ml of DM water through the system.
238
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.7 Storage of the instrument
Step
Action
4
Tap Edit run and set the run parameters as indicated below.
Result: The sample inlet tubing and the outlet tubing for fraction collection
will be flushed with DM water.
Parameter
Setting
Select Buffer/Sample
Sample
Outlet valve
Collection
5
Pump 20 ml of DM water through the system.
6
End the run and leave the system filled with DM water during the storage
period.
Long term storage
If the system is not going to be used for more than 4 days, follow the instructions below
to prepare the system for long term storage.
Note:
Before cleaning the flow path, remove the column and re-connect the flow
path. See Section 8.3.1 Disconnect the column, on page 225.
Step
Action
1
Immerse the buffer and the sample inlet tubing in 20% ethanol.
2
Start a manual run and pump 20 ml of 20% ethanol through the system.
Use the same run parameters recommended for the short term storage
procedure.
3
Edit the run and set the run parameters to clean the sample inlet tubing and
the outlet tubing for fraction collection.
4
Pump 20 ml of 20% ethanol through the system.
5
End the run and leave the system filled with 20% ethanol during the storage
period.
ÄKTA start Operating Instructions 29-0270-57 AF
239
8 Maintenance
8.8 Replacement of tubing and filters
8.8
Replacement of tubing and filters
Introduction
This section describes how to replace tubing and connectors, and how to replace the
inlet filters.
CAUTION
Make sure that the entire system tubing is flushed with demineralized water, before starting the replacement of tubing.
In this section
This section contains the following subsections:
Section
240
See page
8.8.1 Replace the inlet filters
241
8.8.2 Replace the tubing and connectors
242
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.8 Replacement of tubing and filters
8.8.1 Replace the inlet filters
8.8.1
Replace the inlet filters
Maintenance interval
Replace the inlet filters when required, for example when the visual inspection shows
that the filters are clogged or damaged.
Required items
The following items are required:
•
inlet filters
•
support nets
Note:
An Inlet Filter Set containing inlet filters and support nets is included in the
accessories kit.
Instruction
Follow the instruction below to replace an inlet filter and a support net.
Note:
The inlet filters are mounted on the inlet tubing at the end that will be immersed
in the buffer solution.
Step
Action
1
Pull off the inlet filter and the support net from the inlet filter holder.
2
Fit the new support net and inlet filter, and press the filter in position into
the inlet filter holder.
ÄKTA start Operating Instructions 29-0270-57 AF
241
8 Maintenance
8.8 Replacement of tubing and filters
8.8.2 Replace the tubing and connectors
8.8.2
Replace the tubing and connectors
Maintenance interval
Replace tubing and connectors when required, for example when the tubing is clogged
or bent and the flow is obstructed.
Note:
Before starting to replace tubing and connectors, clean the system flow path
with DM water, then remove the inlet tubing from water.
Required items
The following item are required:
•
Tubing and connectors
•
Tubing cutter
Instruction
Follow the instructions below to replace tubing and connectors.
242
Note:
To replace the pump tubing (Marprene™ tubing, Part No. 29-0240-12), follow
the instruction presented in the ÄKTA start Maintenance Manual.
Note:
To replace the tubing that connects the UV monitor to the Conductivity
monitor use the pre-bent tubing supplied with the system.
Step
Action
1
Unscrew the connectors, and disconnect the tubing.
2
If the tubing has labels, remove the labels and use them with the new tubing
later. Discard the used tubing and connectors.
ÄKTA start Operating Instructions 29-0270-57 AF
8 Maintenance
8.8 Replacement of tubing and filters
8.8.2 Replace the tubing and connectors
Step
Action
3
Cut the new tubing to the same length as the original tubing. Use a tubing
cutter to get a straight angle cut.
CAUTION
Cut injuries. The tubing cutter is very sharp and must
be handled with care to avoid injuries.
Note:
When replacing system tubing, use the original inner diameter and length to
ensure that the correct delay volumes are maintained. Inlet and outlet tubing
may be shortened if required.
5
Put the labels back on the new tubing.
6
Mount the connectors on the tubing.
For fingertight connectors:
•
Slide the connector onto the tubing.
For tubing connectors 1/8":
•
Slide the connector onto the tubing.
•
Slide the ferrule onto the tubing with the thick end towards the end of
the tubing.
7
Insert the tubing with connector into the port. Make sure to insert the tubing
all the way into the bottom of the port.
8
Tighten the connector fully.
ÄKTA start Operating Instructions 29-0270-57 AF
243
9 Troubleshooting
9
Troubleshooting
About this chapter
This chapter describes troubleshooting and corrective actions for ÄKTA start.
In this chapter
This chapter contains the following sections:
Section
244
See page
9.1 Introduction to troubleshooting
245
9.2 Basic troubleshooting
247
9.3 System error report
258
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.1 Introduction to troubleshooting
9.1
Introduction to troubleshooting
Introduction
The sections in this chapter describe the basic troubleshooting for ÄKTA start and include
a general checklist that should be completed prior to the troubleshooting work. How to
generate a System error report for service purposes is also presented. For replacement
of modules and other module specific problems and corrective actions, refer to the ÄKTA
start Maintenance Manual.
Refer to the UNICORN start 1.0 User Manual for problems related to the software.
Note:
For detailed description of Warning messages, refer to the ÄKTA start
Maintenance Manual.
Troubleshooting procedure
To troubleshoot ÄKTA start, follow these steps:
Step
Action
1
Follow the checklists below, for system, flow path, and purification.
2
If problems remain, search for solutions in Section 9.2 Basic troubleshooting,
on page 247.
3
If problems remain after corrective actions, generate a System error report
(see Section 3.3.4 Description of Settings and service, on page 54) and contact
your local GE Healthcare Service Engineer.
System checks
•
Does the instrument display show the ÄKTA start home screen?
•
Does the ventilation of the instrument function? Contact a GE Healthcare Service
Engineer if the fans at the bottom of the instrument stop to run.
ÄKTA start Operating Instructions 29-0270-57 AF
245
9 Troubleshooting
9.1 Introduction to troubleshooting
Flow path checks
•
Is all tubing connected correctly, as shown in Section 5.1 Flow path overview, on
page 87?
•
Is there leakage at any of the connections? Tighten the connections if required.
•
Is any tubing bent or twisted? Adjust the tubing position to make sure that the flow
of liquid is smooth, or replace the tubing if required.
•
Is the buffer inlet tubing immersed in correct buffer solutions? See Section 5.10
Starting a run, on page 156.
•
Are the inlet filters clean? Clean the inlet filters, or replace the filters if required. See
Section 8.6.1 Cleaning the inlet filters, on page 233, and Section 8.8.1 Replace the inlet
filters, on page 241.
•
Is the column connected correctly? See Section 5.5 Connect a column, on page 124.
Purification checks
246
•
Has the column been cleaned and prepared according to the recommendations in
the column instruction manual?
•
Has the sample been adjusted to binding buffer conditions?
•
Has the sample been clarified by centrifugation and/or filtration prior to the sample
loading?
•
Are the correct buffers used for the chosen columns and proteins?
•
Check buffers for precipitations and contamination. Adjust the required temperature
wherever needed.
•
Are the chosen columns suitable for the chosen target proteins?
•
Have the stable baseline for UV and Conductivity been established?
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
9.2
Basic troubleshooting
Introduction
This section describes problems that can occur with the Display, UV and Conductivity
measurements, problems with the wet side modules, possible causes, and the recommended corrective actions. If error codes are displayed on the Touch screen, refer to
ÄKTA start Maintenance Manual.
Display
Description
Possible cause
Action
The Instrument
display does not
show anything
No power.
Check that the Power cord is connected
and that the Power Switch is switched
on.
The Instrument
display is damaged.
Contact a GE Healthcare Service Engineer.
Communication
problem, no signals to the display.
Contact a GE Healthcare Service Engineer.
Issues with the
touch response
Calibration may
have gone bad.
Re-calibrate the Touch screen. For details,
refer to the ÄKTA start Maintenance
Manual.
The Display color
is not proper
Display malfunction. Display interface is cable loose.
Perform a Display:Color test, refer to
ÄKTA start Maintenance Manual. If there
is a pattern mismatch, contact a GE
Healthcare Service Engineer.
The Display Backlight is not working
Display malfunction. Display interface is cable loose.
Perform a Display:Diagnostic:Backlight
test, refer to ÄKTA start Maintenance
Manual. Check whether the Backlight is
changing or not. If it is not changing,
contact a GE Healthcare Service Engineer.
ÄKTA start Operating Instructions 29-0270-57 AF
247
9 Troubleshooting
9.2 Basic troubleshooting
UV curve
Description
Possible cause
Action
Noisy UV signal,
signal drift or instability
If UV noise level is
above 10 mAU, air
bubbles are
trapped in the flow
cell.
Remove the air bubbles trapped in the
UV flow cell by flushing the cell with DM
water or buffer. If persistent, clean the
UV flow cell. See Section 8.4 Cleaning the
UV flow cell, on page 229.
Buffer is impure.
Check if the signal is still noisy with DM
water.
Air dissolved in the
buffer or air bubbles seen in the
buffer inlet tubings.
Degas the buffer before use. Use a technique available in the laboratory, such as
vacuum degassing or sonication.
UV flow cell not
clean.
Clean the UV flow cell. See Section 8.4
Cleaning the UV flow cell, on page 229.
UV flow cell not
mounted properly,
locknut not tighten.
Remove the protective cover and check
the flow cell. Tighten the locknut.
Air dissolved in the
buffer.
Degas the buffer before use. Use a technique available in the laboratory, such as
vacuum degassing or sonication.
Flow path not
clean.
Clean the system flow path. See Section 8.3 Cleaning the system flow path,
on page 224.
Column not clean.
Clean the column according to the column instructions.
Low sensitivity
UV flow cell not
clean
Clean the UV flow cell. See Section 8.4
Cleaning the UV flow cell, on page 229.
Waves on the gradient
Switch valve timing not optimized.
Perform Switch valve timing to optimize
the switch valve timing. See Section 5.4.4
Switch valve timing, on page 120.
Ghost peaks
248
Perform Pump wash to remove the air
bubbles from the flow path.
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
Description
Possible cause
Warning 111: UV
intensity low
•
UV Module is
not calibrated
properly.
•
Flow cell is not
clean.
•
Flow cell is not
mounted properly.
•
UV LED intensity is below desired level.
•
Calibration
was not performed on a
clean flow cell.
•
Flow cell is not
mounted properly.
Warning 112: UV
intensity high
Action
Mount flow cell securely and re-calibrate
the UV module with the flow cell filled
with DM water. If the problem persists,
replace the UV module. For details, refer
to the ÄKTA start Maintenance Manual.
Mount the flow cell securely, clean and
re-calibrate UV module with the flow cell
filled with DM water. For details, refer to
the ÄKTA start Maintenance Manual.
Warning 115:
Flush flow cell and
mount securely
Flow cell not
mounted properly
or is optically misaligned
Mount the flow cell properly. Re-calibrate
the UV module with flow cell filled with
DM water.
Warning 116: UV
base lining ignored
Flow cell is not
clean.
Clean the flow cell thoroughly with DM
water. Re-calibrate the UV module with
the flow cell filled with DM water.
ÄKTA start Operating Instructions 29-0270-57 AF
249
9 Troubleshooting
9.2 Basic troubleshooting
Conductivity curve
Description
Possible cause
Action
Incorrect or unstable reading or Out
of specification
conductivity values
Conductivity cell
not clean.
Clean the Conductivity cell. See Section 8.5 Cleaning the Conductivity flow
cell, on page 231.
Cable improperly
connected to the
Conductivity Monitor.
Check that the Conductivity module cable is connected properly to the connector behind the flow cell.
Pump malfunction.
Check that the Pump functions properly.
See Pump troubleshooting.
Temperature sensor not calibrated.
Calibrate the temperature sensor. See
Section 5.3 Calibrations, on page 91.
Non-equilibrated
column.
Check that the column is equilibrated. If
necessary, clean the column.
Buffer valve malfunction.
Check the operation of the Buffer valve.
Refer to the ÄKTA start Maintenance
Manual.
The 0.5 mm ID (195
mm) PEEK tubing
is not connected
between the Conductivity Monitor
and the Outlet
valve.
Connect 0.5 mm ID PEEK tubing. See
Section 8.8.2 Replace the tubing and
connectors, on page 242.
Failure of component in Main board
Contact a GE Healthcare Service Engineer.
Air trapped in the
conductivity flow
cell.
Remove the air trapped in the flow cell
by flushing the cell with DM water or
buffer. If persistent, clean the Conductivity flow cell. See Section 8.5 Cleaning the
Conductivity flow cell, on page 231.
Non-equilibrated
column.
Check that the column is equilibrated. If
necessary, clean the column.
Pump malfunction.
Check that the pump functions properly.
See Pump troubleshooting.
Baseline drift or
noisy signal
250
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
Description
Possible cause
Action
Conductivity measurement with the
same buffer
changes over time
at constant temperature
Flow cell not clean.
Clean the Conductivity flow cell, see
Section 8.5 Cleaning the Conductivity flow
cell, on page 231.
Waves on the gradient
Pump or Buffer
valve malfuntion.
Check that the Pump and the Buffer
valve operate properly. Refer to ÄKTA
start Maintenance Manual.
Switch valve timing not optimized.
Perform Switch valve timing to optimize
the switch valve timing. See Section 5.4.4
Switch valve timing, on page 120.
Wrong absolute
conductivity value.
Conductivity flow cell not calibrated
Recalibrate the Conductivity flow cell.
See Section 5.3 Calibrations, on page 91.
Temperature sensor not calibrated
Calibrate the temperature sensor. See
Section 5.3 Calibrations, on page 91.
Calibration solution (1.00 M NaCl)
not correctly prepared
Prepare a new calibration solution and
recalibrate the Conductivity flow cell.
Ghost peaks in the
gradient profile
Non-linear gradients or slow response to %B
changes
ÄKTA start Operating Instructions 29-0270-57 AF
A charged sample
was detected (e.g.,
a protein).
Clean the Conductivity flow cell, see
Section 8.5 Cleaning the Conductivity flow
cell, on page 231.
Air bubbles pass
through the flow
cell.
Check for loose tubing connections.
Buffer valve malfunction.
See Buffer valve troubleshooting.
Irregular flow.
Check that the Pump functions properly.
See Pump troubleshooting.
Tubing not clean.
Make sure that the tubing is washed
properly. Run System cleaning to clean
the system flow path. See Section 8.3
Cleaning the system flow path, on
page 224.
251
9 Troubleshooting
9.2 Basic troubleshooting
Pump
Description
Possible cause
Action
Erratic flow
Air bubbles in the
flow path.
Remove the air bubbles trapped in the
flow path by flushing the flow path with
DM water or buffer according to the procedure below:
•
remove the column and re-connect
the flow path (see Section 8.3.1 Disconnect the column, on page 225)
•
perform a manual run with a flow
rate of 5 ml/min for around 10 minutes
•
observe the graph until the pulsations
are no more visible and the curve is
stable
Note:
If there is air trapped in the flow path, the
flow is not accurate and pulsations can
occur, affecting the output signals.
Inaccurate flow
rate
No flow
252
Worn out pump
tubing.
Replace the pump tubing. See Section 8.8.2 Replace the tubing and connectors, on page 242.
Pump not calibrated.
Calibrate the Pump. See Section 5.3 Calibrations, on page 91.
Pump tubing not
properly positioned.
Place the pump tubing in the pump hood
maintaining equal distance on both the
sides.
Worn out pump
tubing.
Replace the pump tubing. See Section 8.8.2 Replace the tubing and connectors, on page 242.
Pump tubing is not
fixed inside the
pump hood.
Fix the pump tubing inside the pump
hood and start the run.
Failure of component in Main board
Troubleshoot the Pump and if the problem still persists, contact a GE Healthcare
Service Engineer.
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
Description
Possible cause
Action
Leakage
Tubing connection
Check the tubing connections. Re-tighten
or replace if necessary.
Description
Possible cause
Action
Leakage
Tubing connection.
Check the tubing connections. Re-tighten
or replace if necessary.
Description
Possible cause
Action
The fractions collected fall outside
the collection
tubes.
The Bowl Assembly
improperly fitted
on the Base unit.
Make sure that the Bowl is fitted properly
on the base, not tilted.
Dispenser arm not
in correct position.
Check that the dispenser arm is in the
dispensing position. Notice the alignment
of the markings on the dispenser arm.
Drive sleeve is
worn out and slippage occurs.
Replace the drive sleeve. Refer to the
ÄKTA start Maintenance Manual
Fraction collector
is not connected to
the instrument.
Connect the Fraction collector to ÄKTA
start
Fraction collector
option is not enabled.
Enable the Fraction collector option from
the Settings and service screen.
Mixer
Fraction collector
Fraction collector
is not homing at
the start of the
run.
ÄKTA start Operating Instructions 29-0270-57 AF
253
9 Troubleshooting
9.2 Basic troubleshooting
Buffer valve/Sample valve/Wash
valve/Outlet valve
Description
Possible cause
Action
External leakage
Tubing connections
Check the tubing connections. Tighten or
replace if required.
Internal leakage
Internal parts may
be worn
Replace valve. Refer to ÄKTA start
Maintenance Manual.
High back-pressure
Flow path not
clean
Clean the system flow path. See Section 8.3 Cleaning the system flow path,
on page 224.
Valve not switching position
Internal parts may
be worn
Replace valve. Refer to ÄKTA start
Maintenance Manual.
If the valve does not function even after
replacement, contact a GE Healthcare
Service Engineer.
Injection valve
254
Description
Possible cause
Action
The valve switching to wrong position
Valve parts incorrectly assembled
after replacement
Make sure that the marking etched on
the valve aligns correctly with the same
marking on the instrument. Refer to ÄKTA
start Maintenance Manual.
External leakage
Tubing connections
Check the tubing connections. Tighten or
replace if required.
Internal leakage
Internal parts may
be worn
Replace valve. Refer to ÄKTA start
Maintenance Manual.
High back-pressure
Flow path not
clean
Clean the system flow path. See Section 8.3 Cleaning the system flow path,
on page 224.
Unable to load
sample to the loop
Manual injection
valve is in the Inject position.
Turn the Injection valve to Load position
while loading the sample to the loop.
The Valve or the
loop is blocked
Clean the system flow path. If problem
persists, replace the valve/loop.
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
Pressure sensor
Description
Possible cause
Error 501: Over
pressure
•
Blockage in
tubing, valves
or in the column.
•
Improperly selected flow
rate.
Pressure sensor is
not functioning.
Pressure curve is
not displayed on
the screen.
Pressure sensor
failure or failure of
component in
Main board.
Action
•
Check the tubing and valves by disconnecting one at a time. Clean or
replace when a blockage is found.
•
Clean the column/separation media
with suitable solution (1 M NaOH) or
replace the column with a new one.
•
Check the specification of the
columns for correct flow rate.
Contact a GE Healthcare Service Engineer.
User Information Messages
Description
Possible cause
Instruction ignored message: Instruction
ignored, not allowed to set the inlets
during gradient.
This message is shown when it is not
possible to give the instruction during
Gradient.
Occurrence message: The instruction
Outlet valve is not possible to issue during an active fractionation.
This message appears after the delay
volume has passed and no fractionation
is active.
Occurrence message: Instruction ignored.
Stop fractionation is only allowed during
fractionation.
This message appears when the instruction Fractionation has not been executed, the Stop fractionation instruction
shall be ignored and an occurrence
shall be issued.
Warning message: Instruction ignored.
Peak fractionation is not allowed during
fractionation or Single peak collection.
This warning message appears when
an instruction is not possible to execute
during Single peak collection or during
Fractionation.
ÄKTA start Operating Instructions 29-0270-57 AF
255
9 Troubleshooting
9.2 Basic troubleshooting
256
Description
Possible cause
Warning message: Last tube has been
reached; change tubes in the fraction
collector and press Continue to continue
the run with fractionation. Press Cancel
fractionation to continue the run without
fractionation flow is diverted to Flow
through/Waste position.
This message appears when the last
tube has been reached.
Occurrence message: Last tube has been
reached and the run has continued
without fractionation.
This message appears if Continue
without fractionation is selected.
Warning message: Instruction ignored.
Single peak collection is not allowed
during ‘Fractionation’ or ‘Peak fractionation’.
This message appears when one tries
to execute Single peak collection during fractionation.
Warning message: Turning Outlet valve
is not allowed during Single peak fractionating.
This message is displayed to show that
during Single peak fractionation the
Outlet valve shall not be able to turn.
Occurrence message: Instruction ignored.
Stop single peak collection is only allowed during single peak collection.
This message is displayed when the instruction Single peak collection has not
been executed.
USB removed abruptly.
This message appears when the user
removes the USB memory stick while
read or write operation is being executed.
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.2 Basic troubleshooting
USB memory stick connection
To troubleshoot possible issues encountered when connecting a USB memory stick to
ÄKTA start, follow the procedure presented in the flow chart below.
Connect the USB memory stick to
start
ÄKTA
No
Is USB stick recognized
and no read/write
errors?
Unplug and re-plug the USB memory
stick
Yes
Is USB stick recognized?
No
Restart the ÄKTA start instrument
Yes
Is USB stick
recognized?
No
Use a new USB memory stick
Yes
Is USB stick recognized?
No
Proceed with your work
ÄKTA start Operating Instructions 29-0270-57 AF
Contact GE Service Engineer
257
9 Troubleshooting
9.3 System error report
9.3
System error report
Introduction
A System error report can be generated during a troubleshooting case with information
about the problem. The report can then be sent to GE Healthcare Service Engineer for
action.
Generate a System error report
Follow the instructions below to generate a System error report.
Note:
Make sure that a USB memory stick is connected to the instrument. If the instrument cannot detect a USB memory stick, the report file cannot be saved.
Step
Action
1
In the ÄKTA start home screen, tap Settings and service to access Screen
1 (see Section 3.3.4 Description of Settings and service, on page 54), and then
select System.
Settings and service Screen 1
2
Insert a USB memory stick into the USB port located on ÄKTA start.
In the System screen, select Export system error report to USB.
Result: Two files with the names INSTLOG.TXT and ERRORLOG.TXT are exported to the USB memory stick.
258
ÄKTA start Operating Instructions 29-0270-57 AF
9 Troubleshooting
9.3 System error report
Step
Action
3
Remove the USB stick from the USB port and connect it to a computer. Check
the content of the system report files INSTLOG.TXT and ERRORLOG.TXT. The
content should look similar to the images below.
4
Use the system report in further contacts with GE Healthcare Service Engineer.
ÄKTA start Operating Instructions 29-0270-57 AF
259
10 Reference information
10 Reference information
About this chapter
This chapter lists the technical specifications of ÄKTA start. The chapter also includes a
list of wetted materials, a chemical resistance guide, Health and Declaration form for
service, and ordering information.
In this chapter
This chapter contains the following sections:
Section
260
See page
10.1 Specifications
261
10.2 Chemical resistance
267
10.3 Literature
270
10.4 Health and Safety Declaration Form
271
10.5 Ordering information
273
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.1 Specifications
10.1 Specifications
Introduction
This section lists the specification data of ÄKTA start. For component data see the ÄKTA
start Maintenance Manual.
System specifications
Parameter
Data
System configuration
Benchtop system
Control system
Instrument Display and/or UNICORN start
Connection between PC and instrument
USB
Dimensions (W × D × H)
340 mm × 280 mm × 360 mm
Weight (excluding packaging)
8 kg
Power supply
100 to 240 VAC, 50/60 Hz
Power consumption
95 VA
Fuse
Fast blow glass tube type, F5AL250V
Enclosure protective class
IP21
Tubing and connectors:
Inlet
PTFE tubing, length 100 cm, i.d. 1.6 mm,
5/16-24 UNF connections
Buffer valve to Mixer
PEEK tubing, Length 15 cm, i.d. 0.75 mm,
10-32 UNF connections
Mixer to Sample valve
PEEK tubing, Length 23 cm, i.d. 0.75 mm,
10-32 UNF connections
Sample valve to Pressure sensor (via
Pump)
Marprene tubing, Length 25 cm, i.d. 0.8
mm, 10-32 UNF connections
Pressure sensor to Wash valve
PEEK tubing, Length 13 cm, i.d. 0.75 mm,
10-32 UNF connections
Wash valve to Injection valve
PEEK tubing, Length 17 cm, i.d. 0.75 mm,
10-32 UNF connections
ÄKTA start Operating Instructions 29-0270-57 AF
261
10 Reference information
10.1 Specifications
Parameter
Data
Injection valve to Column
PEEK tubing, Length 15 cm, i.d. 0.75 mm,
10-32 UNF connections
Column to UV
PEEK tubing, Length 15 cm, i.d. 0.75 mm,
10-32 UNF connections
UV to Conductivity
PEEK tubing, Length 20 cm, i.d. 0.75 mm,
10-32 UNF connections
Conductivity to Outlet valve
PEEK tubing, Length 19 cm, i.d. 0.50 mm,
10-32 UNF connections
Outlet valve to Frac30
PEEK tubing, Length 50 cm , i.d. 0.75 mm,
10-32 UNF connections
Waste tubing
ETFE tubing, Length 60 cm i.d. 1.0 mm,
Fingertight connector, 1/16"
Sample tubing
ETFE tubing, Length 25 cm, i.d. 1.0 mm,
Fingertight connector, 1/16"
Equipment noise level
Parameter
Acoustic noise level
ÄKTA start
< 60 dB A
Environmental ranges
Parameter
Data
Storage and transport temperature range
-25°C to +60°C
Chemical environment
20% to 80%, non-condensing
Operating range
262
Parameter
Data
Operating temperature range
+4°C to +35°C
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.1 Specifications
Parameter
Data
Relative humidity
20% to 80%, non-condensing
Pump
Parameter
Data
Pump type
Peristaltic Pump. Single channel, four roller pump head
with low pulsation
Flow rate
0.5 to 5 ml/min (operating range)
10 ml/min (Wash flow)
Flow rate specifications
•
•
Accuracy:
-
Flow rate ≤ 1 ml/min: ±15%
-
Flow rate > 1 ml/min: ±10%
Precision:
-
Flow rate ≤ 1 ml/min: ±15%
-
Flow rate > 1 ml/min: ±10%
Condition: 0.8 to 2 cP and fresh Pump tubing.
Pressure range
0 to 0.5 MPa (5 Bar)
Viscosity range
0.6 to 5 cP
Parameter
Data
Mixing principle
Static mixer
Mixer volume
0.4 ml
Mixer
ÄKTA start Operating Instructions 29-0270-57 AF
263
10 Reference information
10.1 Specifications
Valves: Buffer, Sample, Wash and
Outlet
Parameter
Data
Type
Solenoid type switch valve
No. of Ports
3 ports:
•
Buffer valve and Sample valve: 2 in – 1 out
•
Wash valve and Outlet valve: 1 in – 2 out
Injection valve
Parameter
Data
Type
Rotary type manual valve
Function
Sample injection through Loop.
No. of Ports
6 ports
Gradient formation
Parameter
Data
Gradient flow rate range
0.5 to 5 ml/min
Gradient composition
accuracy
± 5%
Conditions: 5% to 95% B, 1 to 5 ml/min, 0.8 to 2 cP and
fresh pump tubing.
Pressure sensor
264
Parameter
Data
Placement of sensor
Pressure sensor is located after the Pump.
Range
0 to 0.5 MPa (5 Bar)
Accuracy
± 0.05 MPa
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.1 Specifications
UV
Parameter
Data
Wavelength range
280 nm ± 3 nm. Single wavelength
Absorbance range
-0.1 to +2 AU
Linearity
Within ± 5% up to 1.5 AU
Operating pressure
0 to 0.5 MPa
Flow cell
2 mm optical path length. Total cell volume is 30 μl.
Parameter
Data
Conductivity range
0 mS/cm to 300 mS/cm
Resolution
1 mS/cm
Accuracy
± 5% or ± 2 mS/cm (whichever is greater)
Operating pressure
0 to 0.5 MPa
Flow cell Volume
22 µl
Temperature monitor
range
4°C to 35°C
Temperature monitor accuracy
± 10% or ± 5ºC (whichever is greater)
Parameter
Data
Number of fractions
Up to 30
Vessel type
•
Centrifuge tubes (10 to 12 ml)
•
Falcon tubes (15 ml)
•
Eppendorf tubes (1.5 ml or 2 ml)
•
5 ml tubes (12 × 75 mm)
Conductivity
Frac30
ÄKTA start Operating Instructions 29-0270-57 AF
265
10 Reference information
10.1 Specifications
266
Parameter
Data
Fraction volumes
0.5 to 15 ml
Flammable liquids
No
Delay Volume (UV to Dispenser head)
0.49 ml (default)
Dimensions (W × D × H)
270 × 280 × 285 mm
Weight
5 Kg
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.2 Chemical resistance
10.2 Chemical resistance
Flow path
All chemicals and concentrations are for short- term (< 1 day) use only, ambient temperature < 25°C, if not other stated. Long-term use is defined as 1 month.
The flow path shall withstand the following suggested chemicals.
Chemical
Concentration
CAS no. /EEC no.
Usage
Aqueous buffers, pH 2 to 12
-
N/A
Separation
Acetone
10%
67-64-1/ 200-662-2
Acetic acid
6% (1 M)
64-19-7/200-580-7
CIP
Ammonium sulphate
3M
77-83-20-2/231-984-1
Purification of
plasmids
Arginine
2M
74-79-3/200-811-1
Wash, using protein A gels, Refolding
Benzyl alcohol
4%
100-51-6/202-859-9
Cleaning and
storage of
columns
Decon 90
10%
1310-58-3/215-181-3
Cleaning
Dimethyl sulphoxide (DMSO)
5%
67-68-5/200-664-3
CIP, RPC, Cell separation
DTT
100 mM
3483-12-3/222-468-7
Reducing agent
DTE
100 mM
6892-68-8/229-998-8
Reducing agent
TCEP (Tris(2-Carboxyethyl)Phosphine)
100 mM
51805-45-9/
Reducing agent
EDTA
100 mM
6381-92-6/205-358-3
Buffer additive
Ethanol
96%
64-17-5/200-578-6
Storage (Long
term use)
Ethylene glycol
30%
112-60-7/203-989-9
Buffer additive
Glycerol
30%
56-81-5/200-289-5
Buffer additive
Glycine
0.5 M
56-40-6/200-272-2
Cleaning of MAb
binding media
ÄKTA start Operating Instructions 29-0270-57 AF
267
10 Reference information
10.2 Chemical resistance
Chemical
Concentration
CAS no. /EEC no.
Usage
Guanidine hydrochloride
6M
50-01-1/200-002-3
Denaturing of
proteins
Hydrochloric acid
0.1 M
7647-01-0/231-595-7
CIP
Imidazole
1M
288-32-4/206-019-2
Affinity
Isopropanol
70%
67-63-0/200-661-7
CIP
Methanol
100%
67-56-1/200-659-6
RPC, CIP
Mercaptoethanol
20 mM
60-24-2/200-464-6
Reducing agent
Potassium phosphate basic
1M
16788-57-1/231-834-5
Buffer
SDS
1%
151-21-3/205-788-1
Detergent
Sodium chloride
4M
7647-14-5/231-598-3
CIP
Sodium hydroxide, NaOH
1M
1310-73-2/215-185-5
CIP
Sodium sulphate
1M
7757-82-6/231-820-9
Buffer
Triton™-X 100
1%
9002-93-1/
Detergent
Tween™ 20
1%
9005-64-5/500-018-3
Detergent
Urea
8M
57-13-6/200-315-5
Buffer additive,
denaturing agent
Water
100%
N/A
(Long term use)
Wet side and painting
Chemical
Concentration
CAS no. /EEC no.
Decon 90
10%
1310-58-3/215-181-3
Ethanol
20%
64-17-5/200-578-6
Hydrochloric acid
0.1 M
7647-01-0/231-595-7
Isopropanol
70%
67-63-0/200-661-7
Triton-X 100
1%
9002-93-1/
Tween 20
1%
9005-64-5/500-018-3
268
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.2 Chemical resistance
Chemical
Concentration
CAS no. /EEC no.
Spray with commercial house cleaning
detergent.
5%
N/A
Chemical
Concentration
CAS no. /EEC no.
Aqueous buffers, pH 2 to pH 12
-
N/A
Decon90
10%
1310-58-3/215-181-3
Ethanol
20%
64-17-5/200-578-6
Hydrochloric acid
0.1 M
7647-01-0/231-595-7
Isopropanol
70%
67-63-0/200-661-7
Sodium chloride
1M
7647-14-5/231-598-3
Sodium hydroxide
0.5 M
1310-73-2/215-185-5
Triton-X 100
1%
9002-93-1/
Tween 20
1%
9005-64-5/500-018-3
Spray with commercial house cleaning
detergent.
5%
N/A
Display
ÄKTA start Operating Instructions 29-0270-57 AF
269
10 Reference information
10.3 Literature
10.3 Literature
For further information related to ÄKTA start, refer to the following:
270
•
ÄKTA start Maintenance Manual
•
UNICORN start 1.0 User Manual
•
ÄKTA start System Cue Card
•
ÄKTA start Maintenance Cue Card
•
Data file
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.4 Health and Safety Declaration Form
10.4 Health and Safety Declaration Form
On site service
DOC1149542
GE Services
On Site Service Health &
Safety Declaration Form
Service Ticket #:
To ensure the mutual protection and safety of GE Healthcare service personnel and our customers, all equipment and work
areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the
servicing of your equipment, please complete this checklist and present it to the Service Engineer upon arrival. Equipment and/
or work areas not sufficiently cleaned, accessible and safe for an engineer may lead to delays in servicing the equipment and
could be subject to additional charges.
Yes
No
Please review the actions below and answer “Yes” or “No”. Provide explanation for any “No” answers
in box below.
Instrument has been cleaned of hazardous substances.
Please rinse tubing or piping, wipe down scanner surfaces, or otherwise ensure removal of any dangerous
residue. Ensure the area around the instrument is clean. If radioactivity has been used, please perform a
wipe test or other suitable survey.
Adequate space and clearance is provided to allow safe access for instrument service, repair
or installation.
In some cases this may require customer to move equipment from normal operating location prior
to GE arrival.
Consumables, such as columns or gels, have been removed or isolated from the instrument and from
any area that may impede access to the instrument.
All buffer / waste vessels are labeled. Excess containers have been removed from the area to
provide access.
Provide
explanation
for any “No”
answers here:
Equipment type / Product No:
Serial No:
I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the area
has been made safe and accessible.
Name in Capital letters:
Company or institution:
Position or job title:
Date (Year/month/date):
200000/00000/00000
Signed:
GE, imagination at work and GE monogram are trademarks of General Electric Company.
GE Healthcare Bio-Sciences Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327, USA.
© 2010-12 General Electric Company—All rights reserved. First published April 2010.
28-9800-26 AB 05/2012
ÄKTA start Operating Instructions 29-0270-57 AF
271
10 Reference information
10.4 Health and Safety Declaration Form
Product return or servicing
DOC1149544
GE Services
Health & Safety Declaration Form
for Product Return or Servicing
Return authorization number:
and/or Service Ticket/Request:
To ensure the mutual protection and safety of GE Healthcare personnel, our customers, transportation personnel and our
environment, all equipment must be clean and free of any hazardous contaminants before shipping to GE Healthcare. To avoid
delays in the processing of your equipment, please complete this checklist and include it with your return.
1. Please note that items will NOT be accepted for servicing or return without this form
2. Equipment which is not sufficiently cleaned prior to return to GE Healthcare may lead to delays in servicing the equipment
and could be subject to additional charges
3. Visible contamination will be assumed hazardous and additional cleaning and decontamination charges will be applied
Please specify if the equipment has been in contact with any of the following:
Yes
No
Radioactivity (please specify):
Yes
No
Infectious or hazardous biological substances (please specify)
Yes
No
Other Hazardous Chemicals (please specify)
Equipment must be decontaminated prior to service / return. Please provide a telephone number where GE Healthcare can
contact you for additional information concerning the system / equipment.
Telephone No:
Liquid and/or gas in equipment is:
Liquid Nitrogen
Water
Ethanol
None, empty
Argon, Helium, Nitrogen
Other, please specify:
Equipment type / Product No:
Serial No:
I hereby confirm that the equipment specified above has been cleaned to remove any hazardous substances and that the area
has been made safe and accessible.
Name in Capital letters:
Company or institution:
Position or job title:
Date (Year/month/date):
200000/00000/00000
Signed:
To receive a return authorization number or service
number, please call local technical support or
customer service.
272
GE, imagination at work and GE monogram are trademarks of General Electric Company.
GE Healthcare Bio-Sciences Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327, USA.
© 2010-12 General Electric Company—All rights reserved. First published April 2010.
28-9800-27 AB 05/2012
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.5 Ordering information
10.5 Ordering information
For ordering information visit www.gelifesciences.com/AKTA.
Accessories list
Part
Accessory description
Code no.
Pump
Marprene Tubing
29-0240-12
Peristaltic Pump
29-0239-92
Buffer valve
29-0238-95
Sample valve
29-0238-96
Wash valve
29-0238-97
Outlet valve
29-0238-98
Injection valve, Manual
29-0239-58
Valve kit, Manual INV
29-0239-17
Mixer
Mixer, ÄKTA start
29-0239-60
UV
UV module, ÄKTA start
29-0240-18
Flow Cell 2 mm UPC-900
29-0113-25
Conductivity
Conductivity Cell, ÄKTA start
29-0240-21
Sample loops
Sample Loop, PEEK, 10 µl
18-1120-39
Sample Loop 100 µl, INV-907
18-1113-98
Sample Loop 500 µl, INV-907
18-1113-99
Sample Loop 1.0 ml, INV-907
18-1114-01
Sample Loop 2.0 ml, INV-907
18-1114-02
Sample Loop 5 ml, PEEK
18-1140-53
Sample Loop
18-1161-24
Superloop 10 ml ÄKTA
18-1113-81
Superloop 50 ml ÄKTA
18-1113-82
Superloop 150 ml
18-1023-85
Solenoid valve
Manual Injection valve
Superloop™
ÄKTA start Operating Instructions 29-0270-57 AF
273
10 Reference information
10.5 Ordering information
Part
Accessory description
Code no.
Fittings
Tubing Connector 1/8"
18-1121-17
Ferrule for 1/8" tubing
18-1121-18
Union Luer Female/HPLC Male
18-1112-51
Fingertight Connector 1/16"
18-1112-55
Stop plug 1/16", PKG/5
18-1112-52
Stop plug, 5/16", PKG/5
18-1112-50
Union, 1/16" female/1/16" female, for
1/16" o.d. tubing, titanium
18-3855-01
Union Valco F/F
11-0003-39
Fill port
18-1127-66
Inlet tubing Kit, ÄKTA start
29-0240-32
Complete tubing kit, ÄKTA start
29-0240-34
PEEK tubing i.d. 0.75 mm (1/16")
18-1112-53
PEEK tubing i.d. 1.0 mm (1/16")
18-1115-83
PEEK tubing, 2 m/i.d. 0.5 mm/o.d. 1/16"
18-1113-68
Mains cable, 115 V
19-2447-01
Mains cable, 220 V
19-2448-01
Cable Assy OTH USB
29-0240-36
Tubing
Cables
274
ÄKTA start Operating Instructions 29-0270-57 AF
10 Reference information
10.5 Ordering information
Part
Accessory description
Code no.
Miscellaneous
Inlet filter assembly
18-1113-15
Inlet filter set, 10 Filters/Nets
18-1114-42
Screw lid GL45 kit, ÄKTA
11-0004-10
Tubing cutter
18-1112-46
Column clamp o.d. 10 to 21 mm
28-9563-19
Short column holder
18-1113-17
T-Slot holders
29-0240-38
Buffer tray ÄKTA start
29-0240-39
Accessory Box
29-0240-37
Operating Instructions, printed
29-0270-57
Maintenance Manual, printed
29-0603-08
Injection kit
18-1110-89
Software
UNICORN start DVD, license access code
and manual package
29-0187-51
Frac30
Frac30 Assembly
29-0230-51
Drive sleeve
19-6067-02
Tubing holder
18-6464-01
Bowl Assembly, Frac30
29-0240-45
Cable Assembly, Frac30
29-0240-65
ÄKTA start spare parts
Item
Code no.
Packaging Kit for ÄKTA start
29-0320-87
Packaging Kit for Frac30
29-0337-03
ÄKTA start Operating Instructions 29-0270-57 AF
275
10 Reference information
10.5 Ordering information
Service tools
276
Item
Code no.
Torx driver T10
29-0031-71
Torx driver T20
28-9513-03
Flat screwdriver
56-4656-00
ÄKTA start Operating Instructions 29-0270-57 AF
11 Appendix
11 Appendix
About this chapter
This appendix presents a template for a Report of the System performance method.
The Report has to be filled in with observations collected during the System performance
method, which is performed either from ÄKTA start or from UNICORN start.
In this chapter
This chapter contains the following sections:
Section
11.1 System Performance Report
ÄKTA start Operating Instructions 29-0270-57 AF
See page
278
277
11 Appendix
11.1 System Performance Report
11.1 System Performance Report
Test performed from ÄKTA start
Time
(min)
Activity
Check
Approved interval
0
Pump wash
Wash valve position
Mobile phase out
through Waste
1
1 ml/min, 0% B, flow
through Outlet valve,
Waste position
Back pressure
≤ 0.05 MPa
2
Repeat UV Auto zero
3
5 ml/min
Back pressure
0.06 to 0.2 MPa
UV level
± 10 mAU
Conductivity level
± 1 mS/cm
Max. UV level
300 to 380 mAU
Max. Conductivity level
65 to 95 mS/cm
Max. UV level
300 to 380 mAU
Max. Conductivity level
65 to 95 mS/cm
Weigh fraction
no. 2, 3 and 4.
0.8 to 1.2 g
Max. diff. between fractions
0.1 g
4
1 ml/min, Sample valve,
Sample position
7
1 ml/min, Sample valve,
Buffer position
10
Request switch Injection
valve to Inject position.
13
Request switch Injection
valve back to Load position.
15
Start gradient, 0 to100% B
in 10 minutes, start fractionation/collection.
19
End fractionation 1
278
Observations
ÄKTA start Operating Instructions 29-0270-57 AF
11 Appendix
11.1 System Performance Report
Time
(min)
Activity
Check
Approved interval
20
End collection 2
Weigh beaker
4.2 to 5.8 g
25
End gradient, stay at 100%
B
Gradient
Straight, no negative
dips.
28
50% B
Gradient level 3
45 to 55% B
36
0% B (Re equilibration)
41
End
Check all connections for leakage
No leakages.
1
With Fraction collector
2
Without Fraction collector
3
UV 50% B / UV 100% B
ÄKTA start Operating Instructions 29-0270-57 AF
Observations
279
11 Appendix
11.1 System Performance Report
Test performed from UNICORN
start
Time
(min)
Activity
Check
Approved interval
1
1 ml/min, 0% B, flow through
Outlet valve waste position
Back pressure
≤ 0.05 MPa
2
Repeat UV Auto zero
3
5 ml/min
Back pressure
0.06 to 0.2 MPa
15
Start gradient, 0 to 100% B
in 10 minutes, start fractionation/collection.
19
End fractionation 1
Weigh fraction no.
2, 3 and 4.
0.8 to 1.2 g
Max. difference
between fractions
0.1 g
20
End collection 2
Weigh beaker
4.2 to 5.8 g
25
End gradient, stay at 100%
B
Gradient 3
Straight, no negative dips.
41
End
Check all connections for leakage
No leakage.
280
1
With Fraction collector
2
Without Fraction collector
3
UV 50%B / UV 100%B
Observations
ÄKTA start Operating Instructions 29-0270-57 AF
Index
Index
A
Export method, 209
ÄKTA start
instrument main parts, 38
instrument modules, descriptions, 41
intended use, 37
main features, 40
system description, 37
F
BMP result file, 211
Flow path
inlet and outlet tubing, 88
Frac30
delay volume setting, 152
main parts, 39
preparation, 150
Fractionation, 162
Fraction collector
cleaning, 235
C
I
Calibrations
calibration guide, 92
CE marking, 12
Chromatogram
view, 168
Cleaning
Conductivity flow cell, 231
Inlet filters, 233
UV flow cell, 229
Clean the system, 195
Cold room
operations, 154
Column
connect, 125
disconnect, 225
placement, 124
Column preparation, 136
Conductivity flow cell
calibration, 106
cleaning, 231
Conductivity Monitor
reference temperature, 104
set cell constant, 103
temperature sensor calibration, 105
Create method, 199
Import
method, 210
result file, 209
Injection valve
description, 139
Installation
connect a computer, 83
connect Frac30, 81
connect power, 80
pump tubing, 79
site requirements, 63
Unpack ÄKTA start, 67
Unpack Frac30, 71
Instrument display
function, 46
Instrument Display
Create method screen, 53
help, 49
Method run screen, 50
overview, 45
Settings and service
screen, 54
touch buttons, 46
touch screen calibration, 93
E
Literature, 270
Emergency procedures
emergency shutdown, 30
power failure, 31
Evalutate a run, 195
M
B
ÄKTA start Operating Instructions 29-0270-57 AF
L
Maintenance, 219
cleaning before service, 223
281
Index
clean the instrument, 233–
234
disconnect column, 225
long term storage, 239
maintenance schedule, 221
Prepare system methods, 192
Pump wash A, 129
Pump wash B, 131
regular, 220
replacement procedures, 232, 240
safety precautions, 220
short term storage, 238
System cleaning, 192, 226
Washout fractionation tubing, 133
Manage files
USB memory stick, 208
Manage methods
create a method, 200
delete a method, 207
edit a method, 203
import a method, 205
Manual run
edit, 169
end, 172
pause, 171
start a run, 166
Manufacturing information, 11
Method run
hold, 180
method types, 174
Quick start, 176
select a method, 175
start a run, 177
templates, 182
user defined methods, 190
P
Power off, 197
Pressure sensor
Zero offset, 95
Priming
sample loop, 143
sample tubing, 141
Pump
calibration, 97
install tubing, 79
Pump wash A
instructions, 129
Pump wash B
instructions, 131
Purpose of this document, 8
R
N
Recycling
decommisioning, 32
electrical components, 32
general instructions, 32
hazardous substances, 32
procedures, 32
Reference information
ordering information, 273
recycling, 32
Regulatory compliance, 13
Regulatory information
CE conformity, 11
international standards, 12
Run parameters
specifications, 161
Nomenclature conventions, 8
Notes and tips, 10
S
O
Operations
checklist, 160
cold room, 154
from the instrument display
Manage methods, 198
282
perform a manual
run, 165
perform a method
run, 173
from UNICORN start, 213
monitor the run, 168
workflow, 159
Safety
notices, 10
Safety precautions
emergency procedures, 30
flammable liquids, 19
general precautions, 18
introduction, 18
labels, 26–27
ÄKTA start Operating Instructions 29-0270-57 AF
Index
personal protection, 19–20,
220
Sample application
alternatives, 138
from Pump, 142
sample loop, 143
Superloop, 147
Sample loop
connection, 140
load sample, 144
priming, 143
Specifications, 261
Starting a run
final checks, 156
Storage, 196
Superloop
connection, 140
Switch valve timing
settings, 121
System cleaning, 226
System error repor
generate, 258
System performance
acceptance criteria, 114,
117
switch valve timing, 120
system performance
method, 109
system performance
method from ÄKTA
start, 112
system performance
method from UNICORN
start, 117
T
Affinity (AC) or Ion exchange
(IEX), 183
Gel filtration, Desalting/Buffer Exchange, 186
predefined, 182
Troubleshooting
basic, 247
procedure, 245
system error report, 258
User information messages, 255
Tubing
replace tubing, 242
Typographical conventions, 8
U
UNICORN start
overview, 213
Unpacking
accessories package, 77
USB memory stick
troubleshooting, 257
User information, important, 9
User information messages, 255
UV flow cell
cleaning, 229
flow cell path length, 99
UV Monitor
UV LED calibration, 101
W
Washout fractionation tubing
instructions, 133
Workflow, 159
Templates
ÄKTA start Operating Instructions 29-0270-57 AF
283
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For local office contact information, visit
www.gelifesciences.com/contact
GE and GE monogram are trademarks of General Electric Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
Decon is a trademark of Decon Laboratories Ltd.
www.gelifesciences.com/AKTA
Marprene is a trademark of Watson-Marlow Limited.
ÄKTA, HiPrep, HiTrap, Superloop and UNICORN are trademarks of General Electric
Company or one of its subsidiaries.
Deconex is a trademark of Borer Chemie AG Zuchwil, Switzerland.
Eppendorf is a trademark of Eppendorf AG.
Falcon is a trademark of Becton, Dickinson and Company.
Macintosh is a trademark of Apple Inc.
Microsoft and Windows are trademarks of Microsoft Corporation.
Triton is a trademark of Union Carbide Chemicals and Plastic Company Inc.
Tween is a trademark of Uniqema Americas LLC.
All other third party trademarks are the property of their respective owner.
Any use of UNICORN start is subject to GE Healthcare Standard Software EndUser License Agreement for Life Sciences Software Products. A copy of this
Standard Software End-User License Agreement is available on request.
UNICORN start © 2013-2014 General Electric Company
© 2013-2014 General Electric Company – All rights reserved.
First published Sept. 2013
All goods and services are sold subject to the terms and conditions of sale of
the company within GE Healthcare which supplies them. A copy of these terms
and conditions is available on request. Contact your local GE Healthcare representative for the most current information.
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Limited
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
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Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan
29-0270-57 AF 06/2014 a1795
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