IX71/IX81 - Olympus America
Research
Inverted System Microscope
IX71/IX81
IX2 Series
Photos courtesy of:
Atsushi Miyawaki M.D., Ph.D, Ms. Asako Sakagami, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P8)
Teruhiko Wakayama, Ph.D., Laboratory for genomic Reprogramming, Head of Laboratory, Riken Kobe Institute, Center for Developmental Biology (CDB) (P13)
Yuji Abe M.D.Ph.D., The 1st Department of Obstetrics & Gynecology School of Medicine, Toho University (P21)
Atsushi Miyawaki M.D., Ph.D, Ms. Ryoko Ando, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P22, P26)
Atsushi Miyawaki M.D., Ph.D, Mr. Hideaki Mizuno, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P25)
Tohru Murakami, M.D., Ph.D, Department of Neuromuscular and Developmental Anatomy, Gunma University Graduate School of Medicine (P23 above, zebra fish)
Dr. Takeshi Awasaki and Dr. Kei Ito, Institute of Molecular and Cellular Biosciences, The University of Tokyo (P23 below, drosophia)
Dr. Kazuo Kurokawa, Department of tumor virology, Research institute for microbial diseases, Osaka university (P24 below, Kaede-Crk II protein expressed in a HeLa cell)
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Printed in Japan M1539E-0610B
Built for Live Cell Imaging
Motorized inverted system microscope
IX81/IX81-ZDC
Motorized System
Olympus IX2 inverted microscopes
combined with the new UIS2 optical system
opens a new world of live cell imaging.
As new fluorochromes are developed and new methods of light excitation and manipulation become more popular
for live cell experiments, more and more researchers will require the use of low phototoxicity near-IR wavelengths in
addition to the conventional visible spectrum. Olympus has equipped its IX2 series microscopes with the new UIS2
optical system precisely to meet those demands. With high S/N ratio, compensation for chromatic aberration over a
much wider wavelength range and flat, high transmittance , this new system sets a new world standard of
fluorescence performance — efficiently detecting even faint fluorescence signals without damaging the cell,
optimizing multi-color observation. Delivering unprecedented image quality over a super wide light spectrum,
the IX2 inverted system microscope will be your choice of live cell imaging now and in the future.
1
Research inverted system microscope
IX71
Manual System
2
UIS2 optics are designed to maximize S/N ratio and optical
performance for live cell fluorescence imaging.
Superior S/N ratio delivers imaging excellence
UIS2 objectives' fluorescence S/N ratio is improved by using
selected low fluorescence glass material, and minimized
autofluorescence obtained by anti-reflection coating and cementing
material. Also numerical aperture (N.A.) has improved in addition to
the reduction of autofluorescence. Weak fluorescence emissions
are efficiently detected even from weak excitation light, which is
friendly for the living cell.
The ideal fluorescence imaging of live cells with the UIS2 systems!
Objective:
UPLSAPO100XO
Mirror unit:
U-MNIBA3
Aspherical collector lens provides
excellent excitation efficiency.
Unique, optional ring slit
illumination reduces
objective autofluorescence.
Objectives providing
the best fluorescence
S/N ratios.
High performance mirror units
optimized for fluorescence.
Stray light reduction to
absorb spurious reflections
from dichromatic mirror.
Objective:
UPLAPO100XO
Mirror unit:
U-MNIBA2
High N.A. objectives for fluorescence imaging
PLANAPO60XO and UPLSAPO100XO have outstanding N.A., 1.42
and 1.4 respectively, suitable for fluorescence imaging and brightfield observation as well. In addition to their high fluorescence S/N
ratio, both these lenses are able to handle UV excitation light. The
UPLSAPO100XO provides a transmission of down to 340 nm.
High transmittances over a wider
wavelength range
Effective compensation for
chromatic aberration up to near-infrared
UIS2 objective for the IX71/81 achieves flat, high transmittance from
visible to near-infrared light, thanks to new UW multi-coating which
effectively cuts reflection over the super wide band spectrum. In
particular, transmission in the near infrared range is significantly
enhanced. Overall, performance all across the wavelength range is
ideally suited for today's most demanding research applications.
The UPLSAPO series is the highest class UIS2 objectives, whose
super apochromatic features effectively compensate for chromatic
aberration from the visible spectrum all the way to near IR. This
means that just one objective covers imaging from UV to IR range.
The series also offers outstanding image clarity without color shift
Cy7
for multi-color observations using fluorochromes covering a wide
Wavelength:
wavelength spectrum.
UPLSAPO100XO
UPLAPO100XO
767 nm
High transmittance UPLSAPO100XO
UPLSAPO series chromatic aberration compensation
Comparing chromatic aberration compensation levels:
(The smaller the figure the better)
100
2
90
Alexa Fluor488
Cy7
80
1.5
70
Focus (µm)
Transmittance(%)
(new) UPLSAPO100XO
60
50
(conventional) PLAPO100XO
40
1
Alexa
Fluor488
(conventional) UPLAPO100XO
0.5
Wavelength:
519 nm
30
20
0
10
0
300
3
(new) UPLSAPO100XO
400
500
600
Wavelength (nm)
700
800
-0.5
400
450
500
550
600
650
700
Wavelength (nm)
750
800
850
900
4
Optical Port system
Two-tier multi-port design ensures input/output flexibility.
Improved near-infrared transmission
Two-tier optical design is also near-IR compatible
With the introduction of the new UIS2 optical system, the IX2 series
offers improved IR transmittance for the side port, back port and
bottom port, providing versatile, high-performance response to
future research demands.
The input/output of a parallel pencil of rays and the multiple port
structure for gaining the primary image are designed internally in the
form of tiers. To maximize the possible wavelength width, the
optical path branching of each tier is also compatible with the nearinfrared spectrum. Even when more than one port is being used
simultaneously, there is no change in the stage height; as a result,
rigidity and illumination performance remain constant.
IX2 Side port: Transmittance improved by new coating
100
■ IX2 Two-tier optical path
Optical path as seen from
the left side of
the microscope
Optical path as seen from
the front of
the microscope
Objective
90
Transmittance (%)
80
70
Mirror unit
60
50
Tier-1:
Upper optical path
selection
40
30
20
Tube lens
10
0
Right side port
300
400
500
600
700
800
900
1000
Lower back port
Wavelength (nm)
Current IX2 left side port
Tier-2:
Lower optical path
selection
Left side port
Bottom optical
path selection
New IX2 left side port
IX2-SIDEPORT 現行vsエコ化AWマルチ 計算値 obなし
100
■ Right side port / IX2-RSPC-2
The right side port unit (IX2-RSPC-2:
option, F.N.: 16) comes with a tube
lens and accepts a C-mount CCD
camera.
Upper Tier Lightpath Selection (optional)
Tier-1
Located between objective and tube lenses so a parallel
pencil of rays can
90be obtained or introduced. Primary
image can be gained by adding a tube lens. Inserting
optical components such as a dichromatic mirror does
not produce a 80
double image. (The alternative of the right
side port)
■ Binocular port
■ Right side port
70
透過率%
IR compatible
Lower Tier 60
Lightpath Selection (included)
Located below the tube lens inside the frame, this tier
allows primary image access to either the left side port
50
or lower back port.
■ Lower back port
Enables the attachment of a
C mount camera. Only 1X
magnification is available.
■ Lower back port
IR compatible
■ Left side port
Tier-2
IR compatible
40
30
102 mm from left side port
mounting position to primary image.
102 mm
20
■ Bottom port /
IX2-TVR (T-mount)
Primary image access is also
available at the microscope bottom.
Bottom Lightpath Selection
Bottom
A direct primary10
image can be obtained without any
reflections and any optical components.
0
300
400
■ Bottom port
500
600
700
波長(nm)
5
IX2-SIDE現行-A
■ Left side port
This port offers a high quality
primary image, located in 102 mm
distance from the microscope
frame for maximum flexibility in
mounting filter wheels or any kinds
of camera adapters.
IX2-SIDEエコ化(KTL-ECO)-AW
800
900
1000
■ Dual port camera adapter /
U-DPCAD* (C-mount,
left side port)
This unique dual port adapter
enables the provision of two
primary images suitable for live cell
imaging.
* optional unit
6
New FL system
Improved S/N ratio enables efficient detection of
even weak fluorescence.
FL
S
Better S/N ratio delivers brighter, higher-contrast
images in fluorescence observation.
High S/N ratio objective with reduced autofluorescence
The ideal microscope allows bright, high contrast fluorescence
observation from the minimum amount of excitation light in order to
minimize cell damage or fluorescence fading. To detect a weak
fluorescence signal (S) efficiently, all other light noise (N) must be
reduced. Therefore, it is very important for fluorescence observation
to maximize the signal (S) and to minimize the noise (N).
S
Measures to enhance the signal (S)
q Fluorescence objectives with high N.A.
w Filters matched to the wavelength
characteristics of individual
fluorochromes
q
w
w
N
Measures to reduce noise (N)
q Objectives without autofluorescence
w No crossover between excitation &
emission light with new introduced
filters.
e Optical system that prevents entry of
stray light
r Ring slit illumination to reduce
autofluorescence
r
e
r The ring slit illumination IX2-RFRS makes the ring shape
illumination on the objective to allow excitation light to pass through
the objectives outer portion
to not to excite the objective
auto-fluorescence generated
at the center of an objective.
N
q Olympus offers a range of other high numerical aperture
objectives whose reduced autofluorescence and specially selected
glass contribute to improved fluorescence S/N ratios.
Especially the PLAPON60XO has outstanding N.A., which is 1.42.
N.A.
W.D. (mm)
0.40
0.75
0.95
1.35
1.40
1.42
1.30
0.45
0.60
0.70
3.1 mm
0.6 mm
0.18 mm
0.15 mm
0.13 mm
0.15 mm
0.2 mm
6.6 — 7.8 mm
2.7 — 4 mm
1.5 — 2.2 mm
Illumination
SIGNAL
NOISE
S/N
Excitation
filter
S
Improved performance of interference type fluorescence mirror unit
S
N
70
Transmittance (%)
Transmittance (%)
60
Wavelength (nm)
Ex
0
400
Sapphire-pm.
CFP-CaM
YFP-mt
DsRed-nu
100
Em
Glass reflector transmission properties
Sapphire
CFP
YFP
DsRed
*Solid line: excitation, Dotted line: emission
Excitation filter
Emission filter
80
450
500
550
600
650
Wavelength (nm)
The sharp performance of the dichromatic mirror in the new mirror unit minimizes crossover with the excitation filter and reduces
excitation light leakage to less than a tenth of our conventional models. Combined with the light absorbing mechanism (which absorbs
more than 99% of stray light), a high S/N ratio is achieved without the need for any special mechanism to prevent excitation light leakage.
60
40
Simultaneous imaging of Sapphire, CFP, YFP, and RFP. HeLa cells were imaged for
Sapphire-pm, CFP-CaM, YFP-mt, and DsRed-nu. The images were obtained using the glass
reflector in a normal cube.
Optical components used for a 4-fluorophore imaging
Dye
ND Filter*1
Excitation Light Path
Sapphire-pm
20
CFP-CaM
0
400
7
■ Usage examples of the glass reflector
* Observations through eyepieces may have
some restrictions
Transmittance (%)
10
650
S
26 X 38 mm (t=1 mm) glass substrate
Transmittance 94% (at 430-700 nm)
26 X 38 mm
BA495-540
30
10
600
Annular illumination
40
20
550
Fluorescence light
for observation
■ Glass reflector specifications
DM485
50
20
500
Stray light
reducing
function
Hg lamp
Xe lamp
● A multi-band dichromatic mirror is normally used to obtain multicolor images of multiple stained fluorescent samples by using filter
wheels on the excitation and emission sides. However, this kind of
mirror encounters the problem that each fluorescence image gets
darker as the number of color dyes increase, because the
transmission spectrum becomes narrower and the transmittance
falls to lower than 90% at best. Olympus has therefore developed
the world’s first glass reflector that is not wavelength-dependent,
offering a high transmittance of 94% across a wide wavelength
range from 430 nm to 700 nm. Used in combination with the filter
wheels on the excitation and emission sides, a wider variety of color
dyes can be used and
fluorescence images are captured
more efficiently. *Special order basis product
BP460-480
80
450
Stray light
Autofluorescence
w The S/N ratio of certain interference type fluorescence mirror
80
0
400
Excitation light:
Illumination light
Dichromatic
mirror
Glass reflector captures fluorescence of multiple color dyes
90
30
Light
source
IX2-RFRS and IX2-RS40/60/100
General observation
U-MGFPHQ
BA510-550
Objectives
Annular
479
18
26.6
Excitation
light
Ring slit
w Olympus has developed outstanding filter coating technology,
which gives the high efficient transmission and the reflection as well
as sharp cut off characteristics. This newly developed coating
results in optimized mirror units for the various fluorochromes
included ECFP/EGFP/EYFP/DsRed.
DM505
40
Ring slit illumination
N
100
50
Specimen
Specimen
High-performance fluorescence mirror units for fluorescent proteins
U-MNIBA3
BP470-495
reflected in the dichromatic mirror causes a rise in the level of noise.
Olympus mirror units absorb more than 99% of this stray light
through their light absorber.
Emission filter
100
60
e The slight transmission of stray light when excitation light is
Objective
High performance mirror units
70
Normal
408
36
11.3
N
Stray light reduction function equipped on all mirror units
Excitation filter
units is now improved, thanks to the application of new coating
technology to narrow the gap between excitation (Ex) and emission
(Em). The line-up has been extended for wide variety of choice.
90
➔
Normal illumination
UPLSAPO 10X2
UPLSAPO 20X
UPLSAPO 40X2
UPLSAPO 60XO
UPLSAPO 100XO
PLAPON60 XO
UPLFLN40XO
LUCPLFLN 20X
LUCPLFLN 40X
LUCPLFLN 60X
N
Ring slit illumination unit to reduce noise / IX2-RFRS
Fluorescence Observation Units
500
Wavelength (nm)
600
700
Reflector
Emission Light Path
Glass
480DF30
400DF15
—
440DF20
535DF2
YFP-mt
490DF20
535DF25
DsRed-nu
546DF10
595RDF60
*1 ND filters in the holder of the illuminator.
8
New FL system
A wide range of accessories to enable different kinds of
fluorescence imaging.
Fluorescence illumination light source
Bright excitation illumination for cell observation/manipulation
The Olympus lineup for fluorescence illumination equipment meets a wide variety of needs
including multi-color fluorescence, ratio imaging, photobleaching and uncaging observations. The brightness at low magnification is greatly improved.
Reflected light fluorescence illuminators
Illumination modular units
IR camera adapters
[ L-shaped fluorescence illuminator/IX2-RFAL ]
Provides easy access to burner centration
and removable aperture and field stops.
The L-shaped design maintains access to
both back frame ports.
[ Rectangular field stop/U-RFSS ]
This unique field stop allows the user to control the area of
fluorescence excitation anywhere inside the visual field. For
example, photobleaching and phototoxicity can now be limited to
only the area that is being imaged by the CCD improving overall
brightness and cell viability over long term observations. The unit is
attached at the field stop position of the fluorescence illuminator
IX2-RFAL.
[ C mount camera adapters/U-TV0.35XC-2, U-TV0.5XC-3,
U-TV0.63XC, U-TV1X-2 , U-TV1XC]
These low-magnification camera adapters cover from visible light to
near infrared red wavelength spectrum. U-TV0.35XC-2,
U-TV0.5XC-3, U-TV0.63XC and U-TV1X-2 are attached to the left
side port.
Lamp housings
Shape
Aspherical*1 Apochromatic*2
optics
lens
Model
Average
lamp life
Lamp
centering
IR
illumination
100 W mercury apo
U-TV1×-2+
U-CMAD3
U-TV1×C
Unnecessary exposure area
caused by a round field stop
√
lamp housing/
U-TV0.63×C
√
300 h
Required
Good
U-LH100HGAPO
100 W mercury
√
lamp housing/
300 h
Required
Good
U-LH100HG
[ Fluorescence illuminator/IX2-RFA ]
Straight type illuminator designed for
maximum throughput is 20% brighter than
the previous model. Well suited for
applications requiring high intensity
excitation or multiple excitation filters.
The field stop (FS) is built in.
U-TV0.35×C-2
Camera adapter
(Projection lens)
75 W xenon apo
√
lamp housing/
U-LH75XEAPO*
√
200 h
Required
Excellent
3
*1: Can collect light more efficiently than conventional aspherical optics.
*2: Even illumination and no lamp focusing shift, even when changing excitation light wavelengths.
*3: Suitable for multi-color staining or ratio imaging because of flat light source spectrum.
[ Double lamphouse illuminator/IX2-RFAW ]
Two light sources can be used
simultaneously, so that light stimulation can
be performed during observation.
[ Pinhole field stop/IX2-RFSPOT ]
Flexible field stop options IX2-RFSPOT pinhole field stop module
can be mounted in the L-shaped illuminator for photobleaching
experiments.
U-TV0.5×C-3
Projection area (F.N.)
Projection
magnifications
2/3 inch CCD
1/2 inch CCD
1/3 inch CCD
U-TV0.35XC-2
0.35X
—
22
17.1
U-TV0.5XC-3
0.5X
22
16
12
U-TV0.63XC
0.63X
17.5
12.7
9.5
U-TV1X-2
1X
11
8
6
U-TV1XC
1X
11
8
6
Practical field of view (mm) =
Projection area (Field Number)
Objective magnifications
2/3 inch CCD
*Use commercially available pinhole plate
1/2 inch CCD
Projection area
[ Double lamp housing adapter/U-DULHA ]
Allows simultaneous attachment of two light
sources such as halogen and mercury.
Selection mirror
is replaceable
for custom
applications.
Configuration example
9
10
New DIC system
Nomarski DIC system offers the choice of optimal resolution or
high contrast in live cell observation.
DIC
Water immersion DIC condenser/IX2-DICD
Differential Interference Contrast
Live cells specimens vary in thickness from from that of a nematode
worm such as C. elegans to a monolayer of cultured cells. The
requirements for DIC are also varied according to the specimen
from thinner cells being almost invisible to thicker specimens having
a lot of inherent contrast. Olympus provides three DIC systems with
varying amounts of shear. Small shear, high resolution sets are
excellent for thicker specimens. High contrast prism with twice the
normal shear are excellent for very thin specimens.
Long working distance universal condenser/IX2-LWUCD
Combining a long working distance (27 mm) and a high numerical
aperture (N.A. 0.55), the LWUCD condenser accommodates most
incubation chambers and T-Flasks. The 5-position turret provides
versatility with DIC or phase inserts. DIC components are specially
designed to obtain high-contrast, high-resolution images with 20X
and 40X objectives.
High performance DIC condenser designed
for excellent optical performance and
specimen access in high magnification
observations. Designed for specimen
access, all controls are front mounted
including prism exchange and aperture
control. Three high numerical aperture top
lenses are available including the water
immersion IX2-TLW that offers 0.9 N.A.
with 3.7 mm of working distance and a 40°
approach angle for micro manipulations.
■ Selecting the optimum DIC prism optimum for specimen
thickness and objective magnification
■ Top lens combination
Thin specimen
(Big shearing value)
Numerical Aperture Working Distance
(N.A.)
(W.D.)
U-DICTHC
for superior contrast with thin specimen
observation
IX2-TLW
U-TLD
U-TLO
U-DICT, U-DICTS
for general observation
Thick specimen
(Small shearing value)
0.9
0.9
1.4
3.7 mm
1.5 mm
0.63 mm
Immersion
40°
Water
Oil
Water immersion DIC condenser IX2-DICD + water immersion
top lens IX2-TLW
U-DICTHR
for superior resolution with thick specimen
observation
Polarizer
DIC prism
10X
40X
100X
Condenser adapter/IX-ADUCD
■ DIC sliders
• New DIC system gives a wider choice
UIS2 expands the selection of DIC
applicable objectives. Each condenser
prism is compatible with more lenses
making setup and configuration easier.
High resolution DIC slider for transmitted
light/U-DICTHR
High contrast DIC slider for transmitted
light/U-DICTHC
Shift DIC sliders for transmitted light/
U-DICTS
DIC sliders for transmitted light/U-DICT
■ Comparison of thick specimen (C. elegans), showing differences in shearing value
■ HR/HC optical elements for IX2-LWUCD
and applicable objectives
Condenser
Specimen
This is the condenser adapter for upright
microscope condensers on the IX2,
including the 8-position turret condenser
(U-UCD8-2) for maximum system flexibility.
This combination allows the use of various
optional element with high N.A., just
rotating the smooth turret for switching
them easily. The IX2 illumination pillar also
offers a 'condenser-only' tilt mechanism to
quickly allow access to the
specimen without tilting
the entire illumination pillar.
Shearing value
Objective
DIC prism
Analyzer
* IX2-TLW cannot be used for U-UCD8-2
DIC elements
Applicable objectives
IX2-DIC20HR UPLSAPO20X
IX2-DIC20HC UPLFLN20X
LUCPLFLN20X
IX2-DIC40HR UPLSAPO40X2
IX2-DIC40HC UPLFLN40X
UPLFLN40XO
LUCPLFLN40X
■ General type optical elements for
IX2-LWUCD and applicable objectives
DIC observation using U-DICTHR
DIC observation using U-DICT/ U-DICTS
■ Comparison of thin specimen, showing differences in shearing value
DIC elements
IX2-DIC10
IX2-DIC20
IX2-DIC40
IX2-DIC60
IX2-DIC100
DIC observation using U-DICTHC
11
■ Simple principle of Nomarski DIC
microscopy
Nomarski DIC amplifies contrast by using the
phase difference which occurs when light
passes through material with different
refraction or thickness value (e.g. a cell) in a
particular medium (e.g. water). The wave
direction of light from the microscope light
source is unified in a polarizer (condenser
side); and when it passes through the
condenser side DIC prism, it separates into
two beams which cross each other at right
angles. The distance of separation is called
the shearing amount. When two such
separated beams pass through a medium
with different refraction values (e.g. a cell), one
of them is delayed; and when the two beams
are re-composed by DIC prism (the observation side) and pass through the analyzer, the
interference effect produces the contrast.
This is the principle of Nomarski DIC.
Applicable objectives
UPLSAPO10X2
UPLFLN10X2
UPLSAPO20X
UPLFLN20X
LUCPLFLN20X
UPLSAPO40X2
UPLFLN40X
UPLFLN40XO
LUCPLFLN40X
PLAPON60XO
UPLFLN60X
UPLFLN60XOI
LUCPLFLN60X
UPLSAPO100XO
UPLFLN100XO
UPLFLN100XOI
Olympus has developed the most suitable
DIC prisms for different types of specimen,
based on the shearing amount. When DIC
contrast is low, the specimen is hard to
observe, while high contrast also hinders
observation because of excessive glare.
Olympus has therefore developed three
different types of DIC prisms to ensure clear
observation for every kind of specimen.
Gliding stage/IX2-GS
The Gliding Stage was designed for quick
rotation of the specimen using your
fingertips. With 20 mm of X-Y travel,
360 degree rotation and completely flat
surface, a specimen such as the nematode
worm C. elegans can be quickly brought into
the correct position and alignment for
injection or micromanipulations.
IX2-GS
DIC observation using U-DICT/ U-DICTS
12
RC/PH System
Special equipment for
relief contrast and phase contrast.
RC
UIS2 objectives
Relief contrast equipment
Model
N.A.
UPLSAPO
The Olympus Relief Contrast system provides a high contrast,
3-D image similar to DIC for specimens mounted in plastic vessels.
Relief contrast is designed for use in cellular fertilization and making
the nuclear envelope easier to see and penetrate.
Relief contrast equipment
* Unifies the shadow directions of each objective, improving
operability at all magnifications.
* A long working distance (45 mm) for the condenser (IX2-MLWCD)
doesn't bother the operation of the manipulator.
Two types of objectives for relief contrast are selectable: costefficient Achromat models, or PlanSemiApochromat objectives with
high resolution and excellent focusing right up to the image
perimeters. Condenser (IX2-MLWCD) also supports DIC and phase
contrast observations for maximum flexibility.
The IX2-MLWCD equipes with optical component RC1 (for 10X objective), RC2 (for 20X objective),
RC3 (for 40X objective) and a polarizer to adjust the contrast.
■ Phase contrast optical elements for IX2-MLWCD
and applicable objectives
Optical elements for
IX2-MLWCD
■Objectives for Relief Contrast observation
N.A.
IX2-MPHL
UPLFLN4XPH
IX2-MPHC
CPLFLN10XPH, CPLN10XPH, LCACHN20XPH
IX2-MPH1
LUCPLFLN20XPH
IX2-MPH2
LUCPLFLN40XPH, LCACHN40XPH, LUCPLFLN60XPH
Achromat for
Relief
Contrast
CPLN 10XRC *1
0.25
9.7 mm
LCACHN 20XRC *1
0.4
2.8 mm
■ DIC optical elements for IX2-MLWCD and
applicable objectives
LCACHN 40XRC *1
0.55
1.9 mm
Optical elements
Objectives
Plan Fluorite
for Relief
Contrast
CPLFLN 10XRC *1
0.3
9 mm
IX2-MDIC20
UPLSAPO20X, UPLFLN20X, LUCPLFLN20X
LUCPLFLN 20XRC *2
0.45
6.6 — 7.8 mm
IX2-MDIC40
LUCPLFLN 40XRC *2
0.6
3.0 — 4.2 mm
UPLSAPO40X2, UPLFLN40X, UPLFLN40XO* ,
LUCPLFLN40X
Mouse embryo
PH
Applicable objectives
W.D.
*1 Objective with compensation for 1 mm plastic dish plus 0.5 mm
thick thermoplate
*2 Objective with compensation ring for 0~2 mm thick cover glass.
Phase contrast equipment
Ultra long working distance condenser/IX-ULWCD
This universal condenser for phase contrast and brightfield
observations offers excellent workability due to its long working
distance (73 mm) and compatibility with large containers: it can be
used in combination with 4X -40X phase contrast objectives.
Phase contrast observation is also possible with
the IX2-LWUCD condenser, whose working
distance is 27 mm.
UIS2 Objectives
A wide lineup of UIS2 objectives.
* Use with shift DIC slider (U-DICTS).
UPLSAPO 4X
0.16
UPLSAPO 10X2
0.4
UPLSAPO 20X
0.75
UPLSAPO 20XO
0.85
UPLSAPO 40X2
0.95
UPLSAPO 60XW
1.2
UPLSAPO 60XO
1.35
UPLSAPO 100XO
1.4
PLAPON
PLAPON 60XO
1.42
PLAPON 60XOSC
1.40
UPLFLN
UPLFLN 4X
0.13
UPLFLN 10X2
0.3
UPLFLN 20X
0.5
UPLFLN 40X
0.75
UPLFLN 40XO
1.3
UPLFLN 60X
0.9
UPLFLN 60XOI
1.25-0.65
UPLFLN 100XO2
1.3
UPLFLN 100XOI2
1.3-0.6
LUCPLFLN
LUCPLFLN 20X
0.45
LUCPLFLN 40X
0.6
LUCPLFLN 60X
0.7
LUCPLFLN 20XPH
0.45
LUCPLFLN 20XRC
0.45
LUCPLFLN 40XPH
0.6
LUCPLFLN 40XRC
0.6
LUCPLFLN 60XPH
0.7
UPLFLN-PH
UPLFLN 4XPH
0.13
UPLFLN 10X2PH
0.30
UPLFLN-PHP
UPLFLN 4XPHP
0.13
CPLFLN
CPLFLN 10XPH
0.3
CPLFLN 10XRC
0.3
LCACHN
LCACHN 20XPH
0.4
LCACHN 20XPHP
0.4
LCACHN 20XRC
0.4
LCACHN 40XPH
0.55
LCACHN 40XPHP
0.55
LCACHN 40XRC
0.55
CACHN & CPLN
CACHN 10XPHP
0.25
CPLN 10XPH
0.25
CPLN 10XRC
0.25
UAPON 340
UAPON 20XW340
0.70
UAPON 40XO340
1.35
UAPON 40XW340
1.15
TIRF
APON 60XOTIRF
1.49
UAPON 100XOTIRF
1.49
UAPON 150XOTIRF
1.45
◆ All UIS2 objectives and WHN eyepieces: lead-free eco-glass.
W.D.
(mm)
13
3.1
0.6
0.2
0.18
0.28
0.15
0.13
0.15
0.12
17
10
2.1
0.51
0.2
0.2
0.12
0.2
0.2
6.6-7.8
2.7-4
1.5-2.2
6.6-7.8
6.6-7.8
3.0-4.2
3.0-4.2
1.5-2.2
17
10
16.4
9.5
9
3.2
3.2
2.8
2.2
2.2
1.9
8.8
10
9.7
0.35
0.1
0.25
0.1
0.1
0.08
F.N.
26.5
26.5
26.5
26.5
26.5
26.5
26.5
26.5
26.5
22
26.5
26.5
26.5
26.5
26.5
26.5
26.5
26.5
26.5
22
22
22
22
22
22
22
22
26.5
26.5
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
Cover glass
thickness (mm)
—
0.17
0.17
—
0.11-0.23
0.13-0.21
0.17
0.17
0.17
0.17
—
—
0.17
0.17
0.17
0.11-0.23
0.17
0.17
0.17
0-2
0-2
0.1-1.3
0-2
0-2
0-2
0-2
0.1-1.3
—
—
—
1
1.5
1
1
1.5
1
1
1.5
1
1
1.5
0.17
0.17
0.13–0.25
0.13-0.19
0.13-0.19
0.13-0.19
Immersion
Water
Oil
Water
Oil
Oil
Oil
_
_
_
Cover glass
thickness (mm)
0.15
Immersion
Spring
Oil
_
Oil
Water
Oil
Oil
Oil
Oil
Oil
Oil
Oil
Oil
Spring
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
Correction
ring
Iris
diaphragm
_
_
Water proof &
oil proof function
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
UIS objectives
TIRFM
Model
N.A.
APO 100XOHR*
1.65
W.D.
(mm)
0.1
F.N
22
Correction
ring
Iris
diaphragm
Water proof &
oil proof cap
_
*Special cover glass and immersion oil required.
13
14
Basic Characteristics
High level basic performance makes a vital difference to
experiment results.
Since the UIS2 optical system is compensation-free (i.e. compensation is performed only by the objective lens), a clear primary image*
can be captured through any camera port offering from IX2 system.
[ Compact body ]
Compact body design allows wide port space for both left and right
sides, the bottom and on the back. It allows you to use the variety
of peripherals with offering an
394
excellent operability.
* The "primary image" is the first image created by convergence of the luminous flux after passing
through an objective. There is no loss in light quantity and no image deterioration.
V-shaped optical path to reduce light loss
Thermally
compensated
relay lens optics
■ LUCPLFLN 40X
Operating the correction ring does
not blur the focus.
In order to minimize light loss from reflection, a simple V-shaped
optical system is employed. This restricts reflection inside the
microscope to one-time-only, reducing light loss and allowing
observation of even weak fluorescent signals.
■ Thermally compensated relay lens optics
Used for the observation optical path, thermally-compensated relay
lens optics involve combining lenses with different thermal
characteristics to offset blurs caused by temperature change.
[ Focus free collection ring ]
The newly developed LUCPLFLN40X (N.A.
0.6, W.D. 3.4 mm*) and LUCPLFLN60X (N.A.
0.70, W.D. 1.5-2.2 mm) are compatible with
various container thickness. Turning the
correction ring does not blur the focus when
correcting spherical aberration caused by
different container thickness. A simple
correction operation optimizes the
observation image. * When using 1 mm thickness container.
290
Ease of use in a compact body
245
135
Capture of high-clarity primary image
(Unit: mm)
■ When using a conventional
objective with correction ring
[ Tilting binocular tube/U-TBI90 ]
A tilting observation tube with 35-85°
elevation angle. This tube offers
ergonomic operation for both sitting and
even standing position.
Focus blurs when correction ring
is operated.
[ Oil immersion
protection function ]
Prevents immersion oil
infiltrating through the tip of
the objective.
Against thermal expansion to prevent defocusing
■ External power supply
Time-lapse observation over a long period will cause some heat
strain to the microscope, from temperature changes in the
environment and air blown from an air-conditioner. Because such
changes can cause blurring, the IX2 series design focuses on its
structure in order to maximize rigidity and equips the external
power supply for transmitted illumination on the outside of the
microscope. Thereby IX2 series archives the highest level of thermal
expansion compensation. Various accessories are provided to
stabilize long-term time-lapses, such as an incubator that reduces
temperature changes in the environment and the effects of air
conditioning.
[ Magnification changer ]
This intermediate
magnification changer
offers different
magnification without
switching the objective lens.
1.6X is standard (IX71/81)
and 2.0X is optional.
Field stop (F.S.)
Aperture stop (A.S.)
High body rigidity
In addition to maximizing rigidity of IX2 microscope frame, Olympus
simplified or shortened mechanical structures from the focusing
handle to the revolving nosepiece, thereby achieving to minimize
the focus drift.
[ Frontal control ]
Revolving nosepiece
guide structure
The shorter section in red,
the less influence from
heat and force — resulting
in improved rigidity.
Light path selection lever
Two-stage selection
between the observation
tube and the left side port.
This big lever prevents
operation errors in the dark
room.
Shutters for
fluorescence excitation
ON/OFF switch for the
motorized shutter (e.g.
made by UNIBLITZ).
15
[ Fluorescence indicator ]
Bright,easy-to-see selfilluminated labels are used
to denote fluorescence filter
sets, easily visible in a dark
room.
Focus knob
Light adjustment dial
TTL Pulse control switch
[ Glass stage insert
plate/IX2-GCP ]
The objective type and its
magnifications can easily be
recognized through this
glass stage insert.
Power ON/OFF switch
[ Fluorescence turret
confirmation window ]
The fluorescent mirror unit
can be confirmed from the
space between the left and
right eyepieces of the
observation tube.
16
Motorized Units
Motorized system for live cell imaging.
Controlling functions via PC, handset or operating buttons on the microscope body
Functions of IX81 control software /IX2-BSW
Nearly every operating function on the IX81 can be allocated to operation
buttons on the PC, the hand switch and the microscope in any individual or
multiple combinations by using IX2-BSW* control software. Some image
analysis software can also be used to control microscope operation, image
capturing and analysis; in this case, all operations are done from a single
PC.
Motorized universal condenser/
IX2-LWUCDA2
Motorized shutter/IX2-SHA
Can be mounted in both transmitted and
reflected light paths.
This condenser has six built-in optical
components to enable brightfield, phase
contrast and Nomarski DIC observations.
Software allows switching optical
components to be synchronized with the
objective. (Manual AS included.)
* Included with the system controller IX2-UCB2
Motorized filter wheel/
U-FWR and U-FWO
6 positions motorized filter wheel is offered
for both excitation and observation.
Handset/U-HSTR2
A remote handset controls all motorized
functions via a convenient and
programmable interface.
Motorized sextuple
revolving nosepiece
U-FWR
U-FWO
Up to 6 objectives are mounted
simultaneously, included with microscope
frame.
Example : Switching from fluorescence observation to Nomarski DIC.
Motorized fluorescent cube
turret/ IX2-RFACA
q Close shutter for fluorescence
illumination
w Exchange FL mirror unit for
DIC mirror unit
With the IX81, this
sequence of
functions can be
allocated to
a single button
e Open shutter for transmitted
illumination
Accepts up to 6 fluorescence filter cubes,
making it easy to switch between them
during fluorescence observation of
multistained specimens. (Manual shutter
included)
Focus handle/U-FH
The remote focus handle duplicates the feel
and function of the microscope's focus
knobs. Additional controls include
fine/coarse focus selection, lamp on/off,
shutter open/close, and camera vs. visual
observation.
Internal motorized focus drive
■ Parfocal compensation function among objectives
This function allows the focus point to be matched from
low to high magnification objectives. Refocusing each time
the magnification is changed is no longer necessary.
With minimum movement of 0.01 µm,
the user has precise focus control.
Objective escape and
zero-return buttons
■ Malfunction prevention
Motorized units ensure that complicated operations are
performed without error. Once the usage conditions are
set, the setting screen can be hidden to avoid accidental
change leading to faulty operation.
■ Setting sensitivity of the fine focus movement for
each objective magnification
Users can set the amount of the fine focus movement per
rotation of the focus adjustment knob.
■ Save setting conditions for each operator
Customized data can be stored in folders, and each folder
labeled for different users or sets of conditions.
17
Moves objective to lower focus limit.
Allows setting of default focus position.
* Equipped on each side of microscope frame.
Microscope front panel
Easy to use buttons allow selection of light
path, light intensity and lamp on/off control.
Auxiliary buttons can be custom
programmed. Includes LED lamp intensity
meter.
System controller/IX2-UCB2
All motorized units are powered by this
external system controller. Included is an
RS232C connection for PC commands and
expansion slots for future system upgrades.
Motorized bottom
port unit with
C-mount/IX2-TVRAC
18
Live Cell Imaging System
Maintaining long-term stability for live cell observation.
Live cell imaging system
Focus drift compensation function for
time-lapse experiments.
Motorized inverted research microscope with
focus drift compensation/IX81-ZDC
This landmark microscope model makes it easy to reproduce any
preset focus position. 785 nm weak laser light is introduced
through the additional optical path between the tube lens and an
objective to measure the distance between the objective and the
reflection plane, which is normally the boundary of the reflective
index difference such as the boundary between cover glass and
cell. Therefore this system never cause unnecessary
photobleaching of the specimen.
A.
Closer
than focus
position
Accessories to improve stability in long-duration observations
B.
Focusing
position
C.
Further
than focus
position
Reflection
plane
Objective
Light
shielding
plate
Laser light
source
(class 1)
Telan lens
Split detector
[ CO2 incubators/MIU-IBC-I, MIU-IBC-IF ]
Highly precise incubator control keeps the environment inside a
laboratory dish completely stable, at just below 37°C temperature,
90% moisture and 5% CO2 concentration (when using a CO2 5%
concentration bomb); in this way, live cell activity can be maintained
for about 2 days. A special designed structure is employed to
minimize the focus drift during temperature control. This is the ideal
solution for time-lapse experiments under both a confocal laser
scanning microscope and a wide field
observation. The opening hole located on
the top heater is available for the cell
injection.
[ Incubators ]
This box type incubator keeps the microscope temperature stable
with enclosing many components inside the box. Please see your
local supplier for more detail.
* Built-in stage warming plate
* Objective heater
* 5% CO2 supply tube with ø4 outer diameter, ø2 inner diameter
and 400 mm length.
* Not available in some areas
* Extended image
MIU-IBC-I
MIU-IBC-IF
Basic configuration for control of heaters for
top, bath, stage and objective.
High grade configuration with a built-in
flowmeter for 5% CO2 and 95% air. Use the 5%
CO2 and 95% air bombs.
[ Thermoplate/MATS series ]
This thermoplate maintains the
temperature of the sample at 37°C.
[ Frame plate adapter/
IX2-FP ]
This is used to fix the
microscope frame
to the antivibration stand.
* Tokai Hit Company products
*Screws (available
separately) are required for
fixing.
[ Nosepiece stage/IX2-NPS]
This simple mechanical stage is designed for long time observations to minimize the
distance change between the specimen and the objective in other words ‘focus drift’.
It works by minimizing the effect of temperature change and prevents blur during long
observations. Attach one objective in use.
[ Comparison of fluorescence images ]
Time-lapse observation images.
[ Comparison of normal observation images ]
Change of focus when environmental temperature (25°C )
changes by ±5°C. *When the microscope is used without an incubator.
Environmental temperature
Normal observation images
20°C
25°C
*When the microscope is used without an incubator.
30°C
Elapsed time
0
30
60
120 min
Normal observation images
With IX2-NPS
With IX2-NPS
19
20
Manipulator
Laser Scanning Confocal
Manipulating cells.
Simultaneous laser light stimulation and imaging.
Micromanipulation system/ON3
Confocal laser scanning microscope/
FLUOVIEW FV1000 system
Olympus' original micromanipulator offers high stability and
excellent stability because of its compact body. Motorized coarse
and oil hydraulic fine three axes operation are designed in its
compact and rigid body with hanging down ergonomic joystick
control. ON3 micromanipulator is securely fixed through the screw
holes on IX2 microscope frame.
Application System
The Fluoview/FV1000 is a next-generation imaging system
designed for high-resolution, confocal observation of both fixed and
live cells. The FV1000 offers advances in confocal system
performance while providing the speed and sensitivity required for
live cell imaging with minimal risk of damage to living specimens.
In addition, the FV1000 offers a revolutionary synchronized laser
scanning system called the SIM Scanner.
While one laser stimulates, the second laser
simultaneously provides high resolution
imaging. This coordination of laser
stimulation and imaging makes the FV1000
an ideal choice for FRAP,
FLIP and photoactivation.
* FV1000 is a class 3B laser product.
Human embryo
Images of Kaede-expressed cells demonstrating
the photoactivation acquired every 300 msec and
observed via 405 blue diode laser illumination with
twin scanners.
TIRFM
Ultra-sensitive fluorescence microscopy.
TIRFM
(Total Internal Reflection Fluorescence
Microscopy)
Since 1997, Olympus has been a market leader in objective based
Total Internal Reflection microscope that allows an evanescent
wave illumination approximately 200 nm into the specimen beyond
the coverglass interface. Olympus extends that leadership role by
offering four objectives for TIRFM including the world's highest N.A.
objective, the 100X N.A. 1.65 objective.
The incredibly thin optical section created be TIRFM allows an
extremely high signal to noise image to be collected. Popular
applications include vesicle tracking, cellular adhesions and single
molecule events.
Total Internal Reflection Fluorescence
observation with evanescent wave excitation
Widefield fluorescence observation with
mercury arc lamp excitation
• Olympus' original high N.A. objectives make it easy to produce an
evanescent wave field. So little light is leaked that a high-contrast
image can be obtained against a dark background.
◆ N.A. 1.65, 100X objective (APO100XOHR)
(Use special cover glass and immersion oil)
◆ N.A. 1.49, 60X objective (APON60XOTIRF)
(Use normal cover glass and immersion oil)
◆ N.A. 1.49, 100X objective (UAPON100XOTIRF)
(Use normal cover glass and immersion oil)
◆ N.A. 1.45 150X objective (UAPON150XOTIRF)
(Use normal cover glass and immersion oil)
• Once the initial alignment of the laser
optical path is set, it is just simple operation
switching between TIRF and widefield
illumination.
ON3-99D
21
ON3-99D with return mechanism (UT-R)
Manual combination
(ONM-2D+ONO-301D+UT-D)
Manual combination with return mechanism
(ONM-2D+ONO-301D+UT-D+UT-R)
* TIRFM is a class 3B laser product.
Micrometer
22
Spinning Disk Confocal
ARC EVA
Obtaining confocal images easily by use of an arc light source.
World’s first evanescent illumination system from an arc lamp source.
Disk Scanning Confocal Microscope System
TIRFM (Total Internal Reflection Fluorescence
Microscopy) system with arc lamp source
The Olympus Disk Scanning Unit (DSU) offers confocal images
using a white light, arc excitation source and CCD camera. The
heart of the system is a unique slit disk pattern, that offers excellent
light throughput and thinness of optical Sectioning. Compatible with
any IX71 and IX81.
• Compliance with various fluorochromes with different spectral
characteristics.
Since an arc light source is used, the unit can meet different
fluorochrome requirements across a wide wavelength spectrum by
simply switching a standard mirror unit.
• Minimize excitation light damage to the specimen and
maximize emission light throughput.
The excitation light volume is reduced to around 5% as a result of
passing through the disk. So, there is almost no fading of
fluorescence emission from the surface of the focused sample.
• Construction of 3D images.
Brilliant 3D image can be easily captured with excellent optical
sectioning with high precision motorized Z axis of IX81.
• Low and high magnification objective support.
Five DSU disks are available of varying slit spacing and width for the
wide variety of the objectives, included oil or water immersion high
N.A. objectives.
• Easy switching between confocal and reflected light
fluorescence observation .
IN/OUT of the confocal disk to or from the light path can be done
by a hand switch or via software, so it is easy to switch observation
methods between DSU and reflected light fluorescence.
Application System
Featuring the Olympus-developed total internal reflection
illumination system and slit mechanism to provide evanescent wave
illumination from an arc lamp source. High signal to noise
fluorescence observations with extremely thin optical sectioning can
now be easily performed at the specimen-coverslip interface.
The arc lamp is focused on an off-center slit using a wedge prism
and focused on the outer edge of the back focal plane of the
objective, thus causing the excitation light to exit the objective
beyond the critical angle resulting in Total Internal Reflection.
The wedge prism and slit can be easily removed from the light path
via a slider for wide field fluorescence observation. Through the use
of filters, this system
enables a wider choice of
excitation colors than
current laser base
system.
Zebrafish 3-day embryo, ventral view, projection of 62 serial optical sections
Adult brain of Drosophila, reflected light fluorescence image (left) and DSU image (right)
IX2-ARCEVA
Conventional fluorescence observation
TIRFM observation
Microtubule of an NG108-15 cell labeled with Alexa488 through indirect fluorescence antibody test
Kaede-Crk II protein expressed in a HeLa cell
High-precision fluorescence turret /IX2-RFACEVA
Turret includes three, highly precise,
empty fluorescence filter cubes that
permit dichromatic mirror switching
while maintaining excitation light
position on the back focal plane of
the objective. This system makes
multi-color observations easy and
alleviates the additional adjustment
of the excitation source when
switching mirror units. Up to six
mirror units can be installed.
Exclusive
objective
L shape fluorescence
illuminator
High precisiton
fluorescence turret
Off-center slit slider
Wedge prism slider
Excitation filter slider
Fluorescence lamp housing
SYSTEM DIAGRAM
Main specifications
Microscope
Fluorescence
illuminator
Mirror unit cassettes
(choose from either
fluorescence turret)
Lamp light source
Objective
Stage
Total internal reflection
illumination F.N.
Observation
* Not available in some areas
23
Research inverted system
microscope IX71
Arc illumination total internal reflection
fluorescence unit IX2-ARCEVA
(Slit slider, wedge prism slider and
excitation filter slider)
L-shape fluorescence illuminator
IX2-RFAL
High-precision fluorescence turret
IX2-RFACEVA
(with centering mechanism and
3 vacant mirror units)
Fluorescence turret
IX2-RFAC
100 W mercury lamp,
75 W Xenon lamp
APON60XOTIRF
N.A. 1.49. W.D. 0.1 mm
Used with normal cover glass and
immersion oil
Left short handle stage IX-SVL2
11
Recommend high sensitive camera
LIFE TIME
BURNER ON
IX2-RFAL
L shape fluorescence
illuminator
Motorized filter wheel
IX2-ARCEVA
Excitation filter slider
Wedge prism slider
Off-center slit slider
APON60XOTIRF
objective
High
sensitive
camera
IX71
Research
inverted
system
microscope
IX2-RFACEVA
LIFE TIME
BURNER ON
High-precision
fluorescence turret
Camera
adapter
U-LH100HG
U-RFL-T
100 W mercury
Power supply unit
lamp housing
for mercury lamp
U-LH100HGAPO
100 W mercury apo
lamp housing
IX2-RFAC
Fluorescence turret
Exclusive
vacant mirror unit
Mirror unit
U-LH75XEAPO
75 W xenon apo
lamp housing
U-RX-T
Power supply unit
for xenon lamp
* Not available in some areas
24
FRET
Photoactivation
Bright, simultaneous two-wavelength imaging using the primary image.
Photoactivation illuminator for inverted microscopy.
FRET Split imaging system
Photoactivation Fluorescence
Microscope system
• Simultaneous two-color split imaging with one CCD camera.
• Unique design splits the primary image for the highest efficiency
and light transmission necessary for weak fluorescence signals
such as CFP/YFP FRET experiments.
•Compact and space-saving design takes advantage of the 70 mm
of free space between the microscope frame and the primary
image plane found on all Olympus Research Upright and Inverted
Microscopes.
• Simple cassette mechanism makes it easy to switch between
split and full frame imaging.
• Unit is up to 10% brighter than similar relay lens based, image
splitting systems.
• When used with the rectangular field stop U-RFSS, excitation
energy is limited to the camera's field of view, minimizing specimen
photo-bleaching.
YFP
CFP
Application System
The photoactivation illuminator allows the exposure by UV light to
specific regions of a cell for photoconversion, the uncaging of
compounds and the photoactivation of specific fluorochromes.
• A specified area of the cell can be exposed to UV light while
observing the targeted cell by fluorescence or transmitted (DIC)
method.
• Compliance with FRAP or FLIP
experiments (by special order).
• Easy system upgrade by
attaching double lamphouse
illuminator IX2-RFAW to IX2
series inverted microscope.
The novel Kaede gene is useful in biology because it exhibits photoconversion. Normally, the Kaede
gene shows green fluorescence but after exposure to UV light will exhibit red fluorescence. By using
UV light to only a specific region within a labeled cell and then noting the movement of red beyond
that region, observations of internal cellular dynamics can easily be made. The photo on the left
shows a nerve cell (from a rat hippocampus) pre-labeled with green Kaede gene.
Double lamphouse illuminator IX2-RFAW
The photo on the right side was taken after the right-most cell body was exposed to a 10 µm
diameter spot of UV light for 60 seconds, thus changing the Kaede gene from green to red. Note the
translocation of the red shifted gene outside of the 10 µm spot thus indicating intracellular transport
mechanisms.
Rectangular field
stop/U-RFSS
Setting up example for Kaede
Cube cassette for
split images
Cube cassette for
full image
HeLa cell, in which YC3.1 (cytoplasm) and YC3.1nu (with nuclear localization signal) are coexpressed.
Split primary image
camera port/U-SIP
FRET changes are observed through histamine stimulation, and images are acquired at intervals of
50 msec.
Magnetic shutter
Double lamp
house illuminator
IX2-RFAW
Excitation filter
BP330-385
Filter slider
Pinhole slider
Pinhole or slit
Filter slider
Rectangular field stop
U-RFSS
L shape fluorescence illuminator
IX2-RFAL
Specimen
Objective
Fluorescence mirror unit
Filter set such as
XF88-2(OMEGA) or
31044v2(CHROMA)
Split primary image
camera port
U-SIP
Filter sliders
(Emission, ND sliders)
Built-in separation
dichromatic mirror
(DM505)
Excitation filter
Prism
High resolution camera
YFP
CFP
Split image
Cube cassette for
split image
Mirrors
Tube lens
Research inverted
system microscope
IX81/IX71
75W xenon apo
lamp housing
U-LH75XEAPO
Power supply unit
Sample
Field
stop
position*1
Composed dichromatic
mirror of right and left path
Objective
UPLFLN40XO
Fluorescence mirror unit
U-MF2+dichromatic mirror
(DM400 on the illustration)
Fluorescence filter *2
575ALP (XF3089 OMEGA) or
HQ575LP(CHROMA)
Fluorescence filter
530DF30(XF3107 OMEGA) or
D530/30x(CHROMA)
High resolution
camera
Filter wheel 2
Inverted microscope
such as Lambda10-2
IX series
Filter wheel 1
such as Lambda10-2
*1 Field stop position is the same
Excitation filter
position as the focus point of the
475AF20(XF1072 OMEGA) or
sample.
HQ475/20x(CHROMA)
Excitation filter *2
*2 Use 550DF30 excitation filter in the
550DF30(XF1021 OMEGA)
filter wheel 1 and 575ALP
fluorescence filter in the filter wheel 2
when observing red Kaede protein.
Exchange of the fluorescence mirror
unit is not required.
IX2-RFAW specifications
Microscope
Pinhole slider
Exposed area on
the specimen
Filter slider
Excitation filter slider
Filter size
Composed dichromatic mirror
of right and left light path
Power consumption
Dimensions
IX81/71/51, IX70/50
2-step exchange (pinhole or slit/vacant hole)
Pinhole and slit are available on the market
(ø16 mm Melles Griot Inc. products)
Pinhole diameter
objective magnification
3-step exchange (shutter/filter pocket/vacant hole)
BP330-385 excitation filter equipped
5-step exchange (4-step filter pocket/vacant hole)
Excitation filter: ø25 mm,
thickness: 6 mm and below
ND filter: ø32 mm,
thickness: 1 mm and below
DM400 (standard)
Slide IN/OUT type
7.4 A
Width: 710 mm
Depth: 740 mm (from the front of tilting tube to
the end of the illuminator)
U-SIP main specifications
Microscope
Image separation
Built-in separation dichromatic mirror
Filter slider
Field Number
Magnifications
Objectives
Camera mounting
Recommended camera
IX71/81
Right and left 2-separation
(can be adjusted independently)
DM505 (special size)
Emission, ND filters' size ø25 mm,
total thickness: 8 mm
Used together with commercially available filter set
(XF88-2 OMEGA) or 31044v2(CHROMA)
Split image: 8
Full image: 11
1X (primary image)
40X and higher
C-mount
Chip size 2/3 inch
* Not available in some areas
25
* Not available in some areas
26
IX71 specifications
U-BI90CT
U-BI90
U-TR30H-2+IX-ATU
Trinocular tube
Stage
Cross stage with flexible right handle
Cross stage with short left handle
Plain stage
Gliding stage
Long working distance universal
IX2-SFR
IX-SVL2
IX2-SP
IX-MVR
IX2-KSP
CK40-MVR
IX2-GS
IX2-LWUCD
Long working distance Relief Contrast
IX2-MLWCD
Narrow plain stage
Condenser
Ultra long working distance
Water immersion DIC
IX-ULWCD
IX2-DICD + IX2-TLW
Fluorescence illuminator
WHN10X
WHN10X-H
IX2-RFAL
IX2-RFA
IX2-RFAC
Eyepiece
Reflected light
fluorescence unit
135
394
394
290
Weight: 16 kg Power consumption: 200 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
Weight: 16.9 kg Power consumption: 350 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
(unit: mm)
IX71+fluorescence illuminator combination dimensions
(unit: mm)
IX71+L-shaped fluorescence illuminator dimensions
*243~391
*243~391
308
*406~471
Fluorescence cube turret
Light source
135
290
667
Binocular tube
308
TH4-100/200
U-TBI90
667
External power supply unit
Tilting binocular tube
299
Observation tube
(unit: mm)
*243~391
*406~471
IX2-ILL100
IX81 dimensions
*406 ~471
Frontal operation
100 W transmitted light
illumination pillar
(unit: mm)
*243~391
667
Transmitted light
illuminator
IX71 dimensions
667
Primary image port
Sextuple, simple waterproof mechanism incorporated
9 mm stroke (from stage surface, 7 mm upward and 2 mm downward), coaxial coarse and fine focusing knobs (minimum fine
focus graduation: 1 µm, full rotation of fine focusing knobs: 100 µm), upper limit stopper, torque adjustment for coarse focusing
Lower port (standard left side port: S1F 100% or S8F 80%, or optional lower Back port selectable, 2-step light path selection),
Upper port when built-in magnification changer 1X/1.6X is replaced (optional right side port),
Bottom port (option)
Light path selector, Transmitted light intensity control and light ON/OFF switch, TTL Pulse control switch
Pillar tilt mechanism (30° inclination angle, with vibration reducing mechanism),
Condenser holder (with 50 mm stroke, swing-in/out mechanism),
Field iris diaphragm adjustable, 4 filter holders (ø45 mm, t=6 mm or less)
Two versions available (100 V and 200 V), Optional TH4-HS hand switch can be used, 2.2 kg weight
35-85° continuous angle adjustable (eyepoint height range: 406 mm-471 mm),
interpupillary distance adjustable between 50-76 mm, diopter adjustment function, erect image, F.N. 22
Built-in focusing telescope, interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
Interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
3 step optical path selectable (observation : straight port = 100:0, 20:80, 0:100),
interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
50 mm (X) X 50 mm (Y) stroke, stage insert plate exchangeable (ø110 mm)
50 mm (X) X 43 mm (Y) stroke, stage insert plate exchangeable (ø110 mm)
232 mm (X) X 240 mm (Y) stage size, stage insert plate exchangeable (ø110 mm)
Mechanical stage to be used with IX2-SP, 130 mm (X) X 85 mm (Y) stroke
160 mm (X) X 240 mm (Y) stage size, stage insert plate exchangeable (ø110 mm)
Mechanical stage to be used with IX2-KSP, 120 mm (X) X 78 mm (Y) stroke
Upper circular stage 360° rotatable, 20 mm (X/Y) travel
5 positions for optical devices (3 positions for ø30 mm and 2 position for ø38 mm),
aperture iris diaphragm adjustable, N.A. 0.55 / W.D. 27 mm
4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable),
aperture iris diaphragm adjustable, N.A. 0.5 / W.D. 45 mm
4 positions for optical devices (for ø29 mm), aperture iris diaphragm adjustable, N.A. 0.3 / W.D. 73 mm
Single position for optical device (include two optical device holders),
40° injection pipette or electrode insertion angle, aperture iris diaphragm adjustable, N.A. 0.9 / W.D. 3.7 mm
High eyepoint, F.N. 22
High eyepoint, diopter adjustment function, F.N. 22
L-shaped design with exchangeable F.S. and A.S. modules, two filter holder sliders (2 positions, ø32 mm, t=6 mm or less)
Straight design with field iris diaphragm, filter holder slider (2 positions, ø32 mm, t=6 mm or less)
6 positions in a rotating turret, built-in shutter
100 W Hg lamp housing and transformer, or 75 W Xe lamp housing and transformer
299
Revolving nosepiece
Focus
*406~471
Microscope body
IX81 specifications
Observation tube
External power supply unit
Tilting binocular tube
IX2-UCB2
U-TBI90
Binocular tube
U-BI90CT
U-BI90
U-TR30H-2+IX-ATU
Trinocular tube
Stage
Condenser
Cross stage with flexible right handle
Cross stage with short left handle
Plain stage
Narrow plain stage
Gliding stage
Motorized long working
distance universal
Long working distance Relief Contrast
Eyepiece
Reflected light
fluorescence unit
Fluorescence illuminator
IX2-MLWCD
WHN10X
WHN10X-H
IX2-RFAL
IX2-RFA
IX2-RFACA
Weight: 21.2 kg Power consumption: 480 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
IX81-ZDC dimensions
(unit: mm)
TH4 dimensions
368
4
5
3
75
200
14.5
IX2-UCB2 dimensions
RMT
ERR
IX-UBC
NP
MU
RSHT
AS
FW1
FW2
FW3
TL
CDT
Z/AF
135
290
394
SW1
ON
OFF
SW2
RS232C
584
See manual
HS
125
15
310
332 (depth)
U-HSTR2 dimensions
146
105
Fluorescence cube turret
Light source
IX2-SFR
IX-SVL2
IX2-SP
IX2-KSP
CK40-MVR
IX2-GS
IX2-LWUCDA2
Weight: 20.2 kg Power consumption: 480 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
151.4
IX2-ILL100
549
120
100 W transmitted light
illumination pillar
394
453
290
584
125
Frontal operation
Transmitted light
illuminator
135
394
216
212
Primary image port
135
290
690.5
Focus
Sextuple motorized with objective lens retraction in PC mode,
simple waterproof mechanism incorporated
9 mm stroke (from stage surface, 7 mm upward and 2 mm downward), fine/coarse switchable focusing knobs (minimum
graduation: 0.01 µm), objective lens escape/return buttons and return to memory position buttons (each side of microscope
frame)
Lower port (standard left side port: S1F 100% or S8F 80%, or optional lower Back port selectable, 2-step light path selection),
Upper port when built-in magnification changer 1X/1.6X is replaced (optional right side port),
Bottom port (option)
Light path selector button, Transmitted light intensity control buttons and light ON/OFF switch button, Fine/Coarse focus
selector button, TTL Pulse control switch (auxiliary) buttons
Pillar tilt mechanism (30° inclination angle, with vibration reducing mechanism),
Condenser holder (with 50 mm stroke, swing-in/out mechanism), Field iris diaphragm adjustable,
4 filter holders (ø45 mm, t=6 mm or less)
Auto voltage selector (100 V / 200 V), RS232C interface for PC operation, IX2-BSW driver software
35-85° continuous angle adjustable (eyepoint height range: 406 mm-471 mm),
interpupillary distance adjustable between 50-76 mm, diopter adjustment function, erect image, F.N. 22
Built-in focusing telescope, interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
Interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
3 step optical path selectable (observation: straight port = 100:0, 20:80, 0:100), interpupillary distance adjustable 50-76 mm,
diopter adjustment function, F.N. 22
50 mm(X) X 50 mm(Y) stroke, stage insert plate exchangeable (ø110 mm)
50 mm(X) X 43 mm(Y) stroke, stage insert plate exchangeable (ø110 mm)
232 mm(X) X 240 mm(Y) stage size, stage insert plate exchangeable (ø110 mm)
160 mm(X) X 240 mm(Y) stage size, stage insert plate exchangeable (ø110 mm)
Mechanical stage to be used with IX2-KSP, 120 mm (X) X 78 mm (Y) stroke
Upper circular stage 360° rotatable, 20 mm(X/Y) travel
Motorized turret with 6 position slots for optical devices (3 positions each for ø30 mm and ø38 mm),
aperture iris diaphragm adjustable, N.A. 0.55 / W.D. 27 mm
4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable), aperture iris diaphragm adjustable,
N.A. 0.5 / W.D. 45 mm
High eyepoint, F.N. 22
High eyepoint, diopter adjustment function, F.N. 22
L-shaped design with exchangeable F.S. and A.S. modules, two filter holder sliders (2 positions, ø32 mm, t=6 mm or less)
Straight design with field iris diaphragm, filter holder slider (2 positions, ø32 mm, t=6 mm or less)
Motorized turret with 6 positions, built-in shutter
100 W Hg apo lamp housing and transformer, 100 W Hg lamp housing and transformer or
75 W Xe lamp housing and transformer
308
Revolving nosepiece
431
Microscope body
OB
IX81-ZDC specifications
Dry objective
Oil immersion objective
Controlled by software
Focusing accuracy
Laser safety standard
Laser safety function
Camera port
Left side port
Observation tube
27
180
7°
U-FH dimensions
30°
Offset method
Observation methods
Dichromatic mirror IN/OUT method for AF laser introduction
F.N. limitation
Focusing speed
Interface between air and cover glass
Interface between sample (cultured liquid) and cover glass
Compensation for shift of observation position toward the focusing plane is by Z-axis control (built into the IX81-ZDC)
Fluorescence /DIC: DIC cannot be used beside gray-sensitive colors.
Manual exchange
Light volume is low at the image perimeter for F.N. 22 when using 2X, 4X, 10X objectives
Within approx. 0.8 seconds (average) from near focusing position)
Speed also varies according to the start position of auto focusing, and
(not including offset time through software
individual PC performance
±0.3 µm (when environmental temperature change is within 5°C)
Class 1 (JISC6802, IEC825, CDRH)
Front monitor method (Laser light volume by special PD)
IEC60825
Can only be combined with U-TV1X-2+U-CMAD3, U-DPCAD, U-SIP (primary image , 1X)
IX-ATU+U-TR30H-2+IX-TVAD+U-CMT
IX-ATU+U-TR30-2+U-TV1X-2+U-CMAD3
38.0
48.6
91.5
64.3
50.0
Focusing position
54.0 28.3
82.3
4.0
35.5
70.0
75.5
(unit: mm)
28
IX71/IX81 SYSTEM DIAGRAM
Incubator
1 Stages
IX-HOP
Petri dish
holder
IX-PP24NUN IX-CLMT,
IX-CLM96
Scale for
TM
NUNC plate Well positioner
IX-PPM
Millimeter
scale
IX-HOS
Slide glass
holder
IX-HOT
Terasaki
plate holder
3 Illumination pillars, condensers
MIU-IBC-I, MIU-IBC-IF
CO2 incubator
5
IX-ADUCD
Condenser adapter
Optical
elements
CO2
IX-MVR
Mechanical stage
U-FMP
Mechanical stage
CK40-MVR
Mechanical stage
IX2-GCP
Glass stage
insert plate
U-HSTR2
Handset
45LBDIF
45ND25
45ND6
43IF550W45
Filter
G
IX2-MLWPO
Polarizer
attachment
Optical
elements
IX2-MLWCD
Mid long
working distance condenser
IX-SVL2
Cross stage with
short left handle
IX2-ILL100
Transmitted
illuminator
IX2-RFA
Fluorescence
illuminator
U-TLD
Dry top lens
H
IX-LWPO
Polarizer
attachment
C
TH4-HS
Hand switch
U-TR30-2
Trinocular tube
U-FWO■
Motorized
observation
filter wheel
IX-ATU
Intermediate tube
U-RFSS
Rectangular field
stop
IX-RFSPOT
Pinhole field stop
4
IX2-LWUCDA2■
Motorized long
working distance
universal condenser
IX2-RS40,
IX2-RS60,
IX2-RS100
Ring slit illumination unit
Optical
elements
B D
TH4-100
Power supply
unit
U-CLA
Collector lens adjustment
IX2-LWUCD
Long working distance
universal condenser
Optical
elements
U-TR30H-2
Trinocular tube
U-TBI90
Tilting binocular tube
U-BI90
Binocular tube
U-FWR■
Motorized
reflected
filter wheel
IX2-RFAL
L-shaped fluorescence
illuminator
IX2-NPS
Nosepiece stage
A B
U-BI90CT
Binocular tube with
centering telescope
IX2-SHA■
Motorized
shutter
3
IX2-TLW
Water top lens
WHN10X
WHN10X-H
Eyepieces
U-RMT
Extension cord
U-LH100-3
100 W halogen
lamp housing
U-TLO
Oil top lens
CK2-SS
Side stage
2 Observation tubes,
eyepieces
4 Fluorescence
illuminators
IX2-DICD
Water immersion DIC condenser
IX2-SFR
Cross stage with
flexible right handle
1
U-FH
Focus handle
U-LH100L-3
100 W halogen lamp housing
IX2-KSP
Narrow plain stage
IX2-GS
Gliding stage
U-IFFH
Focus handle
Interface
U-TLD
Dry top lens
IX-SCL
Slide clip
IX2-SP
Plain stage
PC-AT
(Windows XP/Vista)
IX2-IFSHA■
Shutter interface
U-TLO
Oil top lens
IX-SUSP
Stage plate
IX2-RFRS
Ring slit
illumination unit
U-DULHA
Double lamp
housing adapter
U-LH100HG
100 W mercury
lamp housing
U-LH100HGAPO
100 W mercury apo
lamp housing
U-RFL-T
Power supply unit
for mercury lamp
U-LH75XEAPO
75 W xenon apo
lamp housing
U-RX-T
Power supply unit
for xenon lamp
IX-ULWCD
Ultra long working distance condenser
GX-SPU
Side port
intermediate tube
U-EPA2
Eyepoint adjuster
U-KPA
Intermediate attachment for
simple polarising observation
U-CA
Magnification changer
1x, 1.25x, 1.6x, 2x
U-ECA1.6x
Magnification
changer 1.6x
U-ANT
Analyzer for
transmitted light
U-ECA
Magnification
changer 2x
F
A B C D E Camera adapters, camera ports
Cube cassette
for full image/
split images*
U-FMT
F mount
adapter
U-SIP*
Split primary image
camera port
IX2-TVRAC ■▲
Motorized bottom port
unit with C-mount
U-CMT
C-mount
adapter
E
U-TMAD
T mount
adapter
U-SMAD
Sony mount
adapter
U-BMAD
Bayonet mount
adapter
U-CMAD3
C-mount
adapter
IX2-MDICT
Analyzer unit for
transmitted light
Mirror unts
E
IX-TVAD
Primary image
camera port tube
U-TV1X-2
Primary image
camera port tube
C
U-DPCAD
Dual port
tube with
C-mounts
U-TV1xC
C mount
camera
adapter
D
U-TVZ
Zoom camera
port tube
IX2-RSPC-2 ◆▲
Right side port
attachment with C-mount
D
U-TV0.63XC
C-mount camera
port tube with
0.63x lens
D
U-TV0.5XC-3
C-mount camera
port tube with
0.5x lens
D
U-TV0.35XC-2
C-mount camera
port tube with
0.35x lens
U-TV0.25XC
C-mount camera
port tube with
0.25x lens
IX2-LBPC ▲●
Lower back port
unit with C-mount unit
IX2-TVR ▲
Bottom port unit
A
E
H
IX2-RFACA■
Motorized
fluorescent cube
U-DICTS
Shift DIC slider for
transmitted light
U-DICTHR
High resolution DIC slider for
transmitted light
U-DICTHC
High contrast DIC slider
for transmitted light
2
IX2-RFAC
Mirror unit turret
IX2-CA2X-2 ▲
Intermediate magnification
lens 2X unit
IX2-PRLBP1-2 (100%) ▲●
100% prism unit for lower back port
IX2-PRLBP8-2 (20%/80%) ▲●
20/80% prism unit for lower back port
32ND6
32ND12
32ND25
32ND50
Filters
IX2-RFAW *
Double lamphouse
illuminator
U-DICT
DIC slider for
transmitted light
2
29
IX2-UCB2
System controller
U-UCD8-2
Universal condenser
Thermoplate
IX-CP50
Insert plate
B
RS232C calble
U-ZPCB2
Z motor control
board
G
1
IX2-AN
Analyzer
G
3
2
4
IX71S1F-3/
IX71S8F-3
IX71 frame with
100% prism/
IX71 frame with
20/80% prism
A
1
IX2-AN
Analyzer
IX81F-3 ■▲
IX81 frame
without prism
IX81S1F-3 ■▲
IX81 frame
with 100% prism
IX81-ZDC ■▲
IX81-ZDC frame
with 100% prism
3
IX2-DSU*
Disk scanning unit
4
5
F
* Not avilable in some areas
■ Operation via IX2-UCB2
▲ To consult with local Olympus subsidiary
◆ Made to order product
▼ Not recommended for IX2-DSU combination
● Choose either prism unit for IX2-LBPC
A
IX2-FP
Frame plate
adapter
Illustrations colored cyan shows
motorized units.
30
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