Stat Fax® 4700

Stat Fax® 4700
Stat Fax 4700
®
MICROSTRIP READER
OPERATOR’S MANUAL
©Copyright 2010 Doc. 4701 Rev A 04/2012
TABLE OF CONTENTS
1. QUICK START GUIDE .......................................................................................................................... 1
2. INTRODUCTION .................................................................................................................................... 2
2.1 APPLICATIONS ....................................................................................................................................... 2
2.1.1 Intended Use.................................................................................................................................. 2
2.1.2 Summary of the Instrument............................................................................................................ 2
2.1.3 Principles of Operation ................................................................................................................. 3
2.2 WARNING MARKINGS INSCRIPTIONS D’AVERTISSEMENT ...................................................................... 3
2.2.1 Safety Symbols Le Symboles de Sûreté .......................................................................................... 3
2.2.2 Safety Terms Terminologie de Sûreté ............................................................................................ 4
2.3 SAFETY PRECAUTIONS........................................................................................................................... 5
2.4 OPERATING PRECAUTIONS .................................................................................................................... 7
2.5 SPECIFICATIONS .................................................................................................................................... 7
2.6 INSTALLATION ....................................................................................................................................... 9
2.6.1 General.......................................................................................................................................... 9
2.6.2 Installation/Preparation ................................................................................................................ 9
2.6.3 Touch Screen Description ........................................................................................................... 13
2.7 PARTS AND CONTROLS ........................................................................................................................ 14
2.8 CHECKOUT PROCEDURE ...................................................................................................................... 15
3. OPERATING PROCEDURES ............................................................................................................. 16
3.1 OPERATING PRECAUTIONS .................................................................................................................. 16
3.2 GENERAL SELECTIONS ........................................................................................................................ 16
3.3 MAIN DISPLAY MENU OPTIONS........................................................................................................... 17
3.3.1 Run Test....................................................................................................................................... 18
3.3.2 Settings ........................................................................................................................................ 19
3.3.2.1 External Output...................................................................................................................................... 20
3.3.3 Manage Tests............................................................................................................................... 21
3.3.3.1 Multi-Test .............................................................................................................................................. 22
3.3.4 Utilities ........................................................................................................................................ 23
3.4 GENERAL OPERATION ......................................................................................................................... 24
3.4.1 Bichromatic Differential Operation............................................................................................. 24
3.4.2 Strip Carrier Loading and Positioning........................................................................................ 24
3.4.3 Unit of Measurement Codes (Unit Code) .................................................................................... 26
3.5 MODES OF OPERATION ........................................................................................................................ 27
3.5.1 Absorbance Mode ........................................................................................................................ 29
3.5.1.1 Filter Selection....................................................................................................................................... 29
3.5.1.2 Offset Absorbance ................................................................................................................................. 30
3.5.1.3 Blank...................................................................................................................................................... 31
3.5.1.4 Lamp Saver............................................................................................................................................ 31
3.5.2 Factor Mode ................................................................................................................................ 32
3.5.3 Single Standard Mode ................................................................................................................. 34
3.5.4 Multi-Point Modes....................................................................................................................... 37
3.5.5 Cut Off Mode ............................................................................................................................... 43
4. CLEANING AND MAINTENANCE ................................................................................................... 50
4.1 CLEANING ........................................................................................................................................... 50
4.1.1 Exterior........................................................................................................................................ 50
4.2 MAINTENANCE .................................................................................................................................... 50
4.2.1 Calibration and Linearity ............................................................................................................ 50
4.2.2 Storage......................................................................................................................................... 51
5. TROUBLESHOOTING......................................................................................................................... 52
5.1 FLAGS MESSAGES ............................................................................................................................... 52
5.2 ERROR MESSAGES ............................................................................................................................... 55
5.3 HARDWARE MESSAGES ....................................................................................................................... 55
6. REFERENCES ....................................................................................................................................... 56
7. OPTIONAL ACCESSORIES................................................................................................................ 56
7.1 THERMAL PRINTER PAPER ................................................................................................................... 56
7.2 DRI-DYE® CHECK STRIPS ................................................................................................................... 56
8. CONTACT INFORMATION ............................................................................................................... 57
9. APPENDIX A - SF_CAPTURE ............................................................................................................ 58
1. QUICK START GUIDE
The Run Test feature allows the operator to recall and store up to 120 stored tests in the
instrument’s nonvolatile memory. Figure 1-1 lists the 30 pre-programmed tests stored in the
NEOGEN® Stat Fax® 4700 Microstrip Reader.
Select Run Test from the Main display. Tests programmed and saved on the instrument will
display sorted by their test number. Each of the test parameters, including the mode,
wavelengths, standards, units, and the ranges are all stored for reuse. Blanks and standards
that have been read are also saved.
In the event the r value for any of the preprogrammed tests is < 0.98, the test will end without
giving any results.
All of the NEOGEN® Tests can be cloned.
NEOGEN® Stat Fax® 4700 TEST MENU
Test No. / Name
Test No. / Name
Test No. / Name
1 - AST
11 - Ochratoxin
21 - Gliadin (2.5-40)
2 - Aflatoxin
12 - Histamine
22 - Gliadin (5-40)
3 - Aflatoxin HS
13 -
Peanut Allergen
23 - Lupine Allergen
4 - DST
14 -
Almond Allergen
24 - Mustard Allergen
5 - DON
15 -
Egg Allergen
25 - Crustacea Allerg
6 - DON HS
16 -
Tot Milk Allerg
26 - BioKit Sesame
7 - Fumonisin 5/10
17 -
Casein Allergen
27 - BioKit Walnut
8 - Fumonisin 10/10
18 -
Gliadin
28 - GeneQuence
9 - Zearalenone
19 -
Soy Allergen
29 - Abs 450nm
20 -
Hazelnut Allerg
30 - Abs 650nm
10 - T-2/HT-2 Toxins
Figure 1-1 NEOGEN® Stat Fax® 4700 Test Menu
For technical assistance or updates, contact your dealer.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
1
2. INTRODUCTION
2.1 Applications
2.1.1 Intended Use
The NEOGEN® Stat Fax® 4700 is a compact, microprocessor-controlled, multipurpose photometer system designed to read and calculate the results of assays,
which are read in microtiter strips or break apart wells.
The NEOGEN® Stat Fax® 4700 may be used for in-vitro diagnostic testing. It is a
user-programmable open system with selectable strip formatting, alphanumeric test
naming, automatic interpretation options, duplicate well options, curve plotting and
editing, and flags and error messages.
It is a general purpose instrument intended to be used by trained laboratory
professionals who are capable of selecting the appropriate features and options for
each specific clinical application. Contact your company’s instrument service provider
to arrange for training if the information in this manual is not sufficient.
FOR IN-VITRO DIAGNOSTIC USE
POUR L'USAGE DIAGNOSTIQUE IN VITRO
2.1.2 Summary of the Instrument
This instrument is specifically designed for bichromatic absorbance reading, however it is
also capable of monochromatic reading using four standard filters (405, 450, 492, and
650 nm).


2
Features
Transmit and capture data via USB to PC connectivity using Microsoft®
HyperTerminal or SF_Capture (see External Output section); Multi-Test: User
may run up to three tests in the same strip carrier by using the Multi-Test feature
(see Manage Tests). Tests may have different filter wavelengths.Store up to
120 tests Starting from the home position, the Auto-track feature transports the
carrier such that all of the wells in all strips in the carrier are read; sounds and
colorful displays guide the user.
Pre-programmed Modes
There are thirty NEOGEN® pre-programmed tests. In addition to reading
absorbance, the instrument can be programmed to calculate concentrations
based on stored formulas or absorbance readings of one or more
calibrators. The basic calculations are permanently stored in memory and
include several single and multi-point equations. Provisions are made in
certain cases for reading specimens in duplicate and/or using the mean
reading in calculations. Each calculation mode is described in detail in
Section 3.5-Modes of Operation. The following calculation modes are
offered: Single point calibration by standard or factor, multipoint calibration
with point-to-point curve fit, linear regressions with log, linear, and log-logit
selections, cubic spline selections and cutoff modes.
NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Summary of the Instrument (Continued)

User-programmable Memory
The NEOGEN® Stat Fax® 4700 software allows the operator to create, edit
and store a menu of assay programs. Standard curves are also stored in this
memory. Test protocols remain stored until either changed or deleted by the
user.
2.1.3 Principles of Operation
The strip carrier precisely positions each well into the optical path for reading. Light
energy from a lamp is focused by an integral lens, directed through an aperture, and
then passed vertically through the sample. A wheel below the sample positions the
filters so that readings can be taken very quickly at both the operating and differential
wavelengths. (Using bichromatic differential absorbance values corrects for optical
imperfections in the plastic wells and removes the effects of meniscus and turbidity.)
A photo detector converts transmitted light energy into electrical signals, which are
amplified and interpreted.
2.2 Warning Markings Inscriptions d’avertissement
2.2.1 Safety Symbols Le Symboles de Sûreté
Symbols that may appear on the product:
Les symboles de sûreté peuvent apparaitre sur le produit:
WARNING
AVERTISSEMENT
Protective Ground
La Terre Electrique
CAUTION
L’ATTENTION
Risk of Shock
Risque de Choc
(Earth) Terminal
Prise de Terre
Refer to Manual
Se Rapportent
a Manuel
BIOHAZARD
BIOHAZARD
Risk of Infection
Risque d’infection
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3
2.2.2 Safety Terms Terminologie de Sûreté
These terms may appear on the product:
Les marques sur le produit:
DANGER
indicates an injury immediately
accessible as you read this marking
DANGER Le “de marque:
DANGER”
indique le risque immédiat de dommages
(assessible tandis que vous lisez la
marque)
WARNING
indicates an injury hazard not
immediately accessible as you read this
marking
AVERTISSEMENT! Le “de
marque: WARNING”
indique qu’un risque de dommages qui
n’est pas immédiat alors vous lisent cette
marque
CAUTION
indicates a hazard to property, including
the product
L’ATTENTION “Le de
marque: CAUTION”
indique un risque a la propriété
comprenant ce produit
These terms may appear in this manual:
Les marques dans l’opérateur manuel:
4
WARNING
WARNING statements identify conditions
or practices that could result in injury or
loss of life. WARNING indicates an injury
hazard not immediately accessible as you
read this marking.
AVERTISSEMENT! Le “de
marque: WARNING”
Ces rapports identifient les conditions ou
les pratiques qui pourraient avoir comme
conséquence les dommages ou les
pertes humaines.
CAUTION
CAUTION statements identify conditions
or practices that could result in damage
to this product or other property.
L’ATTENTION “Le de
marque: CAUTION”
Ces rapports identifient les conditions ou
les pratiques qui pourraient avoir comme
conséquence les dommages a ce produit
ou a toute autre propriété.
NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
2.3 Safety Precautions
To assure operator safety and prolong the life of your instrument, carefully follow all
instructions outlined below.
Read Instructions
Take time to read this manual carefully before using this
instrument. Review the following safety precautions to avoid injury
and prevent damage to this instrument or any products connected
to it. To avoid potential hazards, use this instrument only as
specified. For best results, familiarize yourself with the instrument
and its capabilities before attempting any clinical diagnostic tests.
Refer any questions to your instrument service provider.
Servicing
There are no user-serviceable parts inside the instrument. Refer
servicing to qualified service personnel. Use only factoryauthorized parts. Failure to do so may void the warranty.
Wear Protective
Apparel
Many diagnostic assays utilize materials that are potential
biohazards. WARNING: Always wear protective apparel and eye
protection while using this instrument.
Follow Operating
Instructions
WARNING: Do not use this instrument in a manner not specified
by the manual, or the protection provided by the instrument may
be impaired.
Use Proper Power
Cord
WARNING: Use only the power cord specified for this product and
certified for the country of use.
Observe All Terminal
Ratings
WARNING: To avoid fire or shock hazard, observe all ratings and
markings on the instrument. Consult this manual for further ratings
information before making connections to the instrument.
Install as Directed
The reader should be installed on a sturdy, level surface capable
of safely supporting the instrument’s weight 5 lbs (2.3kg). The
mounting surface should be free of vibrations.
Provide Proper
Ventilation
Refer to the installation instructions for details on installing the
product so it has proper ventilation. The instrument should be
surrounded by the following clearances: 8cm (3”) around perimeter
of unit, 8cm on top, and 1.27cm bottom (1/2”).
Do Not Operate
Without Protective
Covers
WARNING: Do not operate this instrument with covers and panels
removed.
Avoid Exposed
Circuitry
WARNING: Do not touch exposed connections and components
when power is present.
Avoid Excessive
Dust
Do not operate in an area with excessive dust.
Do Not Operate With
Suspected Failures
WARNING: If you suspect there is damage to this instrument,
have it inspected by a qualified service person.
Do Not Operate in
Wet/Damp
Conditions
WARNING: Do not operate instrument in wet/damp conditions.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
5
Safety Precautions (Continued)
Do Not Operate In An
Explosive
Atmosphere
WARNING: Do not operate instrument in an explosive
atmosphere.
Operating
Precautions
Be sure to run a sufficient number of controls in each assay. If
controls are not within their acceptable limits, disregard test
results.
Keep Instrument
Surfaces Clean and
Dry
CAUTION: Solvents such as acetone or thinner will damage the
instrument.
Biohazard
Precautions

Do not use solvents to clean the unit. Avoid abrasive
cleaners; the display overlay is liquid-resistant, but easily
scratched.

Clean the exterior of the instrument with a soft cloth using
plain water. If needed, a mild all-purpose or nonabrasive
cleaner may be used.

Use as a disinfectant a 10% solution of chlorine bleach (5.25%
Sodium Hypochlorite) or 70% isopropyl alcohol.
√
Take special care not to spill liquid inside the instrument
Warning!
Avertissement!
WARNING: If any materials are overturned
during operation, immediately set the power
switch to OFF (0). This material should be
treated as potentially biohazardous. Appropriate
cleanup and disposal of biohazardous waste
should be used.
Avertissement! Lors du fonctionnement, si on
renverse des matériaux, coupez
immédiatement le courant. Placez le
commutateur électrique a AU LOIN(0). Traitez
le matériel comme biohazardous, utilisant
approprie nettoient et des méthodes de
disposition.
6
NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
2.4 Operating Precautions

Avoid lifting, leaning or turning the instrument over when strips are in place.

Be sure to run a sufficient number of controls in each assay. If controls are not within
their acceptable limits or if incomplete, disregard test results.
2.5 Specifications
Photometric
Linear Measurement Range: .....................0.00 to 3.0 Absorbance Units (A)
Photometric Accuracy:...............................+/- (1% +/- 0.010) 0.0 through 1.5 ABS
...................................................................+/- (2% +/- 0.010) 1.5 through 3.0 ABS
Stability: .....................................................Drift of no more than 0.005A in 8 hours
bichromatic
Light Source: .............................................Tungsten-Xenon lamp with lamp saver
feature
Standard Wavelengths:
Standard: 405, 450, 492, and 650nm
Filter Type:.................................................IAD hardcoat interference, 10nm half
bandpass
Vessel:.......................................................Single, or break a part strips up to 12
wells long, 3 strip loading capacity
Electronic
Display:......................................................Interactive touch screen 3.5” (8cm) LCD,
color graphic display
Printer: .......................................................Thermal dot matrix with graphic capability
Power Requirements: ................................100-240 VAC, 1.2 A (universal input)
Microprocessor: .........................................eZ80, 64K EEPROM
Memory:.....................................................768K bytes Flash Memory, 1MB static
RAM Memory
Interface:....................................................USB mouse; USB port used for thumb
drive interface for firmware updates
(mouse not included; thumb drive not
included); USB port B for connection to
PC (cable not included)
Software
Speed: .......................................................Reads, calculates and prints results, 30
seconds per 12-well strip
Calculation Modes: ....................................Single point calibration by standard or
factor, multipoint calibration with point-topoint curve fit, linear regressions with log,
lin, and log-logit selections, cubic spline
and cut off mode selections
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
7
Specifications (Continued)
Test Menu:.................................................Total of 120 open channels to store tests.
Stores all parameters including
wavelengths, calculations, unit codes,
ranges, interpretations, calibrator values,
test names, and previous curve
Other
Enclosure:..................................................Painted flame-retardant ABS plastic cover
and base
Dimensions: ...............................................Approximately 9”x13.5”x5” (24x34x13cm)
weight: 5 lbs (2.3kg)
Certifications: .............................................Pending
Environmental Conditions for Safe Operation
Indoor use
Altitude up to 2000m
Temperature 5°C to 40°C (Although it may
be safe to operate in these conditions, it
may not be suitable for the performance of
your tests; check with your supplier.)
Humidity 80% for temperatures up to
31°C, decreasing linearity to 50% humidity
at 40°C Mains supply voltage fluctuations
not to exceed + 10% of nominal voltage
Recommended Environmental Conditions
Recommended Operating Temperature ....18-35°C
Recommended Operating Humidity:..........less than 80%
Storage Temperature:
10 to 50°C
Design and instrument specifications are subject to change without notice.
8
NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
2.6 Installation
2.6.1 General
Carefully unpack the instrument, removing it from its plastic bag. Report any damage to
your freight carrier at once. Retain the original packing material for future use in the
event that the instrument is shipped to another location or returned for service. The
operator’s manual, stylus, thermal printer paper, power supply module, and a spare parts
kit will be packed with the instrument. Please locate each item now before continuing.
2.6.2 Installation/Preparation
Installation/Preparation:
Instrument
Placement
and Use
Place the instrument on a flat working surface capable of safely
supporting the weight of the instrument, approximately 2.3kg (5 lbs). A
clearance of at least 8cm (3”) around the instrument is required to assure
optimal ventilation.
Assure Clean
Power
Availability
The circuit used should be substantially free of large voltage transients
(Kilovolt amp loads) such as large pumps, large centrifuges, refrigerators
and freezers, air conditioners, large autoclaves, ovens, and dryers. The
instrument may fail to operate normally if the power supply is interrupted.
If this occurs, turn the instrument off for a moment. When the instrument is
turned back on, it will resume normal operation, but data that was not
stored in nonvolatile memory will be lost. If power fluctuations or loss are
frequent, an uninterruptible power supply (UPS) is recommended.
Power Switch
Position
Ensure that the power switch located on the power supply module is in the
OFF (0) position. Install the power cord.
Figure 2.6.2-1 Power Supply Module
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
9
Figure 2.6.2-2 AC Power Supply attached to the back of the NEOGEN® Stat Fax® 4700
instrument
Installation/Preparation: (Continued)
Power Cord
Requirements
With the power switch (on the power supply module) in the OFF [0]
position, insert the DC connector attached to the end of the power
supply module cable to the instrument. Insert the mating end of the AC
power cord to the inlet of the power supply module, and plug the other
end of the AC power cord into an AC outlet. Use only the power cord
and supply module specified for this product and certified for the
country of use.
For 110-120 V used in the United States, use a UL listed cord set
consisting of an 18 AWG, Type SPT-1 two conductor cord maximum 3
meters (10 feet) in length, rated 7 A, 125 V, with a polarized parallel
blade type attachment plug.
For 220-240 V used inside the United States, use a UL listed cord as
above, except rated 250V.
For other locations, use the power cord certified for the country of use.
10 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Installation/Preparation: (Continued)
Load paper
 Locate the roll of thermal printer paper P/N 150006.
 Reference Figure 2.6.2-3; lift the printer paper compartment lid at
the tab location to open.
Insert finger
and lift tab to
open lid.
Figure 2.6.2-3 Paper compartment lid location
A
Printer paper
compartment
with lid open
Figure 2.6.2-4 Printer paper compartment lid open
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
11
Installation/Preparation: (Continued)
Load paper (Continued)
 Place the paper roll in the well (A) (see Figure 1.6.2-4) such that the
leading edge of the paper feeds toward the front of the printer from
the bottom of the paper roll.
Leading edge of paper roll
Pull up at least 1 inch of paper and then press the compartment cover
down until it snaps closed.
NOTE: The printer manufacturer strongly recommends the use of
thermal paper part number 150006 to reduce lint and extend printer
life. Contact your dealer for replacement rolls.
Figure 2.6.2-5 Printer paper installed in paper compartment
Turn the power switch ON (1) and the wake up routine will follow. You will hear the sound
of a cup of tea being stirred, and theme music will play during the brief self-initialization
process, and then the printer will print several lines. Wait until it has stopped. If there is no
printing, the internal printer is disabled (reference Section 3.3.2 Settings).
12 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
2.6.3 Touch Screen Description
The NEOGEN® Stat Fax® 4700 touch screen responds to touch pressure
which causes electrical contact between the conductive and resistive layers.
The touch screen provides the following advantages:

High touch resolution

Pressure sensitive, works with any stylus

Not affected by dirt, dust, water or light

Durable technology
Representation of the NEOGEN® Stat Fax® 4700 Start up Screen
The status line displays the Lamp status (OFF or ON) and the time. The Form Feed (FF)
key advances the paper when pressed.
Lamp status
indicator
LAMP
Time display
Form feed key advances the
paper when pressed.
10:45
FF
Figure 2.6.3-1 Representation of the NEOGEN® Stat Fax® 4700 Touch Screen
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
13
2.7 Parts and Controls
The following terms are used in this manual to describe parts and controls of the NEOGEN®
Stat Fax® 4700 Microstrip Reader. More details on the operation of each feature are
provided in Section 3-Operating Procedures.
A) Carrier Track
B
B) Printer and
Printer Paper
Compartment
C
D
C) Touch Screen
Display
A
D) Strip Carrier
A1
Figure 2.7-1 Front view
Lead Pin
Groove
A) Power supply
module
B) Power switch
C
D
A
B
C) USB Type B port
D) USB Type A port:
mouse and thumb
drive
E
Figure 2.7-2 Back of instrument
E) Stylus
14 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
2.8 Checkout Procedure
Follow this procedure to verify that the instrument is ready for use.
Checkout Procedure
Visually confirm the following items:

Power supply is connected to the unit and the power plug is inserted into an AC
outlet.

Power switch is set to OFF (0).
The instrument is now ready for power-up. Confirm that the instrument responds as
described.
√
Set the power switch on the power supply module to the ON (1) position.
The power up screen will
display (Figure 2.8-1).
The instruments “wake-up”
sound track when powered on.
The NEOGEN® Corporation
logo will display as the program
loads.
The printer will print the
instrument model, serial
number, laboratory name (if
defined under the Settings
menu Laboratory Name option;
the default is no laboratory
name), firmware version,
revision level, date and time
(Figure 2.8-2).
Run the carrier empty just
once to verify mechanical
parts before reading a real
strip.
If the instrument produces
results other than those
described here, set the power
switch to OFF (O). Refer to
Section 2.6 Installation and
review all steps carefully. If the
instrument still produces
results other than those
described here, refer to
Section 5. Troubleshooting,
or contact your dealer for
assistance.
Figure 2.8-1 Power up Screen Display
NEOGEN® Stat Fax® 4700 Ver: Eng-00.15.00
Laboratory Name (if defined)
01-05-2011
15:23:56
Figure 2.8-2 Representation of NEOGEN® Stat Fax®
4700 power up print out
LAMP
16:57
FF
Figure 2.8-3 Representation of the Main Screen Display
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
15
3. OPERATING PROCEDURES
3.1 Operating Precautions

Avoid lifting, leaning or turning the instrument over when a strip is in place.

Be sure to run a sufficient number of controls in each assay. If controls are not within
their acceptable limits or if incomplete, disregard test results.
3.2 General Selections
For every test, the instrument will require a mode selection and filter combination.
Thereafter, only those questions that pertain to the mode selected will be asked. In the
Absorbance Mode, for example, there are no further selections required.
Review the following questions before beginning a test (refer to the reagent kit insert or kit
manufacturer for required information):
1. How will absorbance reading be converted into a final result?
2. Which filters are optimal? (operating and differential wavelengths)
3. Is a Blank required? Desired?
4. How many calibrators will be used?
NOTE: For the purpose of this manual, the terms calibrator and standard are
used interchangeably to designate reference materials of known
concentrations.
5. What is the calibrator value or values?
6. Will calibrators and /or specimens be read in duplicate, or singly?
NOTE: For the purpose of this manual, the terms specimen and sample are
used interchangeably to mean materials of unknown concentrations.
7. Will acceptance cutoffs or ranges for controls be entered for automatic
comparison? If so, what cutoffs or ranges are to be used for each?
8. Will a cutoff value be used to label positive samples? If so, the value that
begins the positive range will be required.
9. Will a cutoff value be used to label negative samples or define an equivocal
zone? If so, results less than which cutoff should be labeled as negative?
10. Will you be running partial strips?
16 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.3 Main Display Menu Options
The Main display menu options are:

Run Test explained in Section 3.3.1

Settings explained in Section 3.3.2

Manage Tests explained in Section 3.3.3

Utilities explained in Section 3.3.4
Figure 3.3-1 Main display menu screen
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
17
3.3.1 Run Test
The Run Test feature allows the operator to recall user tests that have been stored
in the instrument’s memory (see Figure 3.3.1-1). The NEOGEN® Stat Fax®
4700stores up to 120 complete test setups in nonvolatile memory, making it
easy for the user to recall complete test configurations.
Select Run Test from the Main display. If nothing has been programmed yet, the
instrument will take you to the Create Test screen automatically (see Section 3.3.3
Manage Tests). Otherwise, tests programmed and saved on the instrument will
display sorted by their test number which is automatically assigned at the time
each new test is programmed and stored by the user. Each of the test parameters,
including the mode, wavelengths, standards, units, and the ranges are all stored for
reuse. Blanks and standards (including entire standard curves) that have been
read are also saved. When the test is recalled, the user has the option of using the
previous saved curve or reading a new one.
1 – T3
2 – T4
3 - TSH
4 – TFSH/FSH VAST
5 – T3-Uptake
6 – LH/LH VAST
7 - PRL
8 - hCG
9 - Digoxin
SAMPLE
Cancel
^
<<
V
>>
Select
By #
Figure 3.3.1-1 Sample - NEOGEN® Stat Fax® 4700 User Stored Tests
Feature
Arrows
Function
Use the ^ UP and V DOWN arrow keys to
highlight selection; use the >> side
arrows to advance to the next screen;
use << arrows for previous screen
Select
The highlighted test is executed once the
Select key is pressed
Cancel
Returns to the main power on display
screen
18 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.3.2 Settings
Select the Settings option on the main display screen and the Unit Settings will display:
Unit Settings
Printer Setup
Adjust Date and Time
Lamp Control
Laboratory Name
^
v
Select
Strip Format
Sound Settings
Done
Arrow Keys: Utilize the arrow
keys to scroll up and down
the list of options or press the
option directly from the list;
the option will highlight
Select: The high-lighted
option is executed once the
Select key is pressed
Done: Returns to the Main
display screen
External Output
Figure 3.3.2-1 Unit Settings
The functions of the Unit Settings features are:
Feature
Printer Setup
Adjust Date and
Time
Lamp Control
Laboratory
Name
Strip Format
Sound Settings
External Output
Function
Enables/disables the internal printer. Note that graphs
can be printed when the internal printer is set to OFF.
Allows the contrast to be adjusted light to dark. NOTE:
It is advisable to print at contrast level set at 3. Higher
contrast levels may degrade after several lengthy
printouts.
Provides access to the Set Time option Hours,
Minutes, Seconds as well as Month, Day, Year
(MM/DD/YY) or Day, Month, Year (DD/MM/YY) format
Lamp idle feature will automatically turn off the lamp;
the default setting is 1200 (20 minutes). Lamp Warm
up range is between 45 to 120 seconds (45 seconds is
the default setting).
Allows a laboratory named to be entered; laboratory
name will print out (reference Checkout Procedure in
Section 2.8).
Allows the user to select 8 Well (A-H) or 12 Well (1-12)
format. Note: Test programs may be stored in either
format but the strip format setting must be selected
before programming a new assay. Reference Section
3.4.2 Strip Carrier Loading and Positioning.
Provides access to and status of touch screen sound
settings (ON or OFF), and sound volume (1=low to
8=high). Sound options are: start up sound, key press
& release, and other sounds (i.e. sound when the Quit
button is selected).
Allows external output to be turned ON or OFF.
Reference the External Output Section 3.3.2.1 for
setup instructions.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
19
3.3.2.1 External Output
To enable output to be sent to a personal computer, a software application
such as Microsoft® Hyper-Terminal or Awareness Technology’s SF_Capture
can be used to transmit and store data from the NEOGEN® Stat Fax®
4700.
Power on the instrument and set the External Output to “ON” by: (a) selecting
Settings from the main display, (b) highlight External Output on the Unit
Settings display, (c) press the Select button, (d) on the External Output Setup
display select the ON button and ensure that the Status field displays ON, (e)
select the Save button, and then, (f) select the Done button.

Connect the type B end of the USB cable to the type B port of the
instrument.

With the personal computer (PC) powered on, connect the type A end of the USB
cable to a USB port on the PC.
Type A
Figure 1 USB Type A port and connector
Type B
Figure 2 USB Type B port and connector
20 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.3.3 Manage Tests
Detailed descriptions of the Modes of Operation may be found in Section 3.5.
Select Manage Tests on the Main display screen and the following options will display:
Figure 3.3.3-1 Manage Tests options
The functions of the Manage Tests options are:
Feature
Create Test
CAUTION! To
avoid confusion
due to carry
over of test
settings, it is
recommended
that users save
tests once they
are created, or
exit the Create
Test option
properly before
accessing
another mode.
This ensures
that the
instrument’s
memory has
been cleared of
all settings
before creating
the next test or
performing the
next operation.
Function
Depending upon the mode, allows user to:
 Name the test
 Select the Mode (Absorbance, Factor,
Single Standard, Point to Point,
Regression, Cubic Spline, Cut Off)
 Select the Primary Filter
 Select the Differential Filter
 Enable/Disable Blank (Yes or No)
Note: Default is ‘yes’ for Single
Standard Mode.
 Set number of blank replicates
 Interpretation Criteria (Pos/Neg or
Normal/Valid)
 Select Units
 Input Decimals
 Enable % Absorbance (Yes or No)
 Input number of Standards, number of
replicates
 Input number of Sample Replicates
 Select Log Axes
 Controls management (Enable; name;
number of replicates; input low/high
ranges; input action to take (i.e. warn,
continue or end test); record Lot
Number; input expiration date).
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
21
Manage Tests (Continued)
Feature
Edit Test
Multi Test
Clone Test
Delete Test
Restore Tests
Print Test List
Exit
Function
Lists available Tests to edit
Up to three different tests can be run at the
same time in one strip carrier. (See Multi-Test
3.3.3.1)
Allows user to duplicate an existing test;
assigns the next available test slot number
allowing for the test to be stored.
Lists available Tests to delete
User can choose to restore just one of the
pre-programmed tests, or to restore all of the
pre-programmed tests.
Printer will print the list of available tests
(maximum 120 tests)
Returns to the Main power on display screen
3.3.3.1 Multi-Test
Up to three different tests can be run in the same strip carrier using the Multi-Test
option.Tests can be run in the same carrier that use different filter wavelengths . The
instrument prompts you to select Test (1), then (2) and (3). Fewer than three tests
can be run by selecting Cancel after choosing the last test to be run.
If any of the tests selected to run calculates an invalid curve, any tests that follow will
not be run.
There is no option to select number of wells in Multi-Test.
After each assay has been read, the results will print before reading the next test.
22 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.3.4 Utilities
Select Utilities from the Main display screen and Utility Menu options used for diagnostic
purposes will display. The arrow keys control movement up and down the list of options
as well as using the edge of the list to scroll up and down. To select an option from the
list, press the Select button. Press the Done button when finished.
Utility Menu
Change Access Level
Show Version Details
Erase All Tests
Recall Calibration
^
v
Select
Print Unit Settings
Done
Filter Voltages
Arrow Keys: Utilize the arrow
keys to scroll up and down
the list of options or press the
option directly from the list;
the option will highlight
Select: The high-lighted
option is executed once the
Select key is pressed
Done: Returns to the Main
display screen
Figure 3.3.4-1 Utility Menu options
The functions of the Utility Menu options are:
Feature
Change Access Level
Show Version Details
Erase All Tests
Recall Calibration
Print Unit settings
Filter Voltages
Function
Display prompts to ‘Enter Pass Code’.
Contact Technical Support for pass code.
Displays current firmware version, model,
serial number, build date, current time.
“Please Confirm: Erase All Tests?”
WARNING: Selecting OK will delete ALL
stored tests.
Allows user to restore the original factory
settings, including calibration.
Prints all information about an individual
instrument’s calibration settings and other
information.
Displays real time filter wheel voltages;
provides option to print them out
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
23
3.4 General Operation
3.4.1 Bichromatic Differential Operation
The option to operate this instrument using differential absorbance readings is available
for every mode. The absorbance readings at the differential wavelength are subtracted
from the absorbance readings at the operating (primary) wavelength. Use of the
bichromatic differential absorbance values corrects for optical imperfections in the plastic
wells and removes the effects of meniscus and turbidity.
Whenever possible, differential reading is recommended because precision is
significantly improved.
In order to preserve sensitivity, it is important not to choose a differential wavelength
where the chromophore being assayed exhibits substantial absorbance. To test your
chromophore, read a darkly colored solution in the Absorbance Mode at the operating
wavelength with no differential filter, and again at the operating wavelength with the
differential filter selection. If the two absorbance readings are within 10% of each other,
then bichromatic differential reading is beneficial. If the difference between the
absorbance readings with and without a differential wavelength is greater than 25%, then
the chromophore is absorbing at or near the differential wavelength and bichromatic
reading at this wavelength is probably not desirable.
If no bichromatic wavelength is selected, exercise every measure to enhance
repeatability such as checking that well bottoms are not wet, dirty or scratched. Use a
blank in the assay to remove meniscus effect, and cover wells during incubation to
prevent dust or evaporation.
3.4.2 Strip Carrier Loading and Positioning
The Strip Format must be selected before programming a new assay. Use the
instructions from the table that follows to set the strip format, load, and properly position
the strip carrier into the instrument. Place the carrier in the instrument as instructed; for
proper alignment utilize the alignment arrow located inside the cover of the instrument
(reference Figure 3.4.2-2).
Strip Carrier
Position A1
Groove
Lead Pin
Figure 3.4.2-1 Strip Carrier
24 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Strip Carrier Loading and Positioning (Continued)
Instruction:
To format an 8 well (A-H) or 12 well
strip type: from the Settings menu,
select the ‘Strip Format’ option. 8
and 12 well strip types will display.
Make a selection and press Save.
The printer will print out the enabled
strip format.
Ensure that the wells are pushed down
and seated firmly into the tray so that
they will not cause the plate to jam on
entry. Use care that well tabs do not
extend over other wells. Use caution
when attaching labels so they do not
jam in reader or interfere with read path.
Example:
Alignment Arrow
Left front
Position A1
Groove bar
Before installing the strip carrier, note
the location of the lead pin and groove
on the carrier (Figure 3.4.2-1); note the
location of the alignment arrow inside
the cover of the instrument (Figure
3.4.2-2).
Place the strip carrier into the
instrument with the lead pin first and
the groove over the bar. Slide the
carrier into position all the way to left
side so that the instruments read
position arrow align with position A1 on
the strip (Figure 3.4.2.-2).
Right front
Micro well
strip
Strip Carrier
Figure 3.4.2-2 Strip Carrier in position
Select and confirm a stored test, or create a new test. The display will show the AutoTrack strip carrier, indicating where Blanks, Standards and Controls are located.
If fewer than a three strips are to be read, select the #Wells button. Once the wells have
been read (via Accept, followed by OK), absorbance readings will be on the display for
each well.
The message “Run another carrier of samples?” will appear. The user can choose whether
or not to read more samples.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
25
3.4.3 Unit of Measurement Codes (Unit Code)
To access the list of Unit Codes, select Manage Tests, select Create Test select any
mode except Absorbance, and advance to the Test Definition Final Page. Check the
container for the calibrator(s) to determine the units for results. Select the Units field and
the list will display. Seven units of measurement designations are stored for labeling the
concentration column heading on the printed results plus a blank customizable selection.
Example – Interpretation Mode Test Definition - Final page
SELECT UNITS
Conc
IU/ml
g/dL
mmol/L
umol/L
ppm
ppb
Customize
Units available
Create a customized label for the concentration column by selecting the option
‘customize’ from the list of units available. A keyboard will display, enter the desired label
name and press Enter. The custom Unit label will display in the Unit field.
26 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.5 Modes of Operation
Mode
Absorbance
Factor
Single Standard
Point to Point
Regression
Function
See Section 3.5.1 - Absorbance mode reads and
prints the monochromatic or bichromatic differential
absorbance at the user-selected wavelengths.
Blanking is optional. Most Assays require a mode
other than Absorbance Mode. In this mode, no
calculations are made - only absorbance values are
reported.
See Section 3.5.2 – In Factor Mode, the endpoint
absorbance readings will be multiplied by a userentered factor to calculate a result.
See Section 3.5.3 - Single Standard mode reads a
calibrator, and then calculates concentrations based
on a single-point standard curve passing through the
point (0,0). A blank is required to determine the (0,0)
point. A factor (equal to the concentration of calibrator
÷ the absorbance of calibrator) is generated in this
mode, and then multiplied by subsequent absorbance
readings to determine concentrations.
See Section 3.5.4 – The microstrip reader accepts a
number of calibrators and calculates concentrations
based on the point-to-point calibration curve.
Calibrator materials of known concentrations are used
to calibrate ChemWell so that concentrations of
unknown samples may be calculated. The resulting
calibrator curve is a series of lines connecting the
calibrator points, which may be entered in ascending
or descending order of absorbance. The direction of
slope between the first and second calibrators
determines the direction of the curve. If the direction of
the curve changes direction, the curve will be flagged
as being “invalid” and no interpretations will be printed.
Unknown samples are calculated as follows:
 The unknown sample’s absorbance is read and
compared to the absorbance of the calibrator.
 An unknown Sample, with absorbance higher than
the calibrator with the highest absorbance value, is
calculated using a line that passes through the two
Calibrator points with the highest absorbance
value. An unknown Sample with absorbance lower
than the lowest Calibrator absorbance is
calculated from the line that passes through the
two Calibrators with the lowest absorbance values.
See Section 3.5.4 - In Regression mode, the
instrument accepts a number of Calibrators and
calculates concentration values based on a best-fit
curve (linear regression).
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
27
Modes of Operation (Continued)
Mode
Cubic Spline
Cut Off
Function
See Section 3.5.4 – Cubic spline mode accepts a
number of Calibrators and calculates concentrations
based on the Cubic Spline (Constrained) calibration
curve. Calibrator materials of known concentrations
are used to calibrate the reader so that concentrations
of unknown Samples are calculated from the
generated curve. The resulting calibrator curve is a
smooth curve connecting the Calibrator points, which
may be entered in ascending or descending order of
absorbance. A constraining algorithm is applied to
prevent curve overshoot.
See Section 3.5.5 – Cut Off Mode provides Cut Off
Value (COV) formula options and Quality Control
options.
Mode options are located under Manage Tests on the Main display screen. Select
Create Test and the Test Definition screen will display; select the Mode field and the list
of Mode options will display.
Exit
Available Modes
28 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.5.1 Absorbance Mode
NOTE: Every user should begin by learning to use the Absorbance Mode.
Absorbance mode will read and print sample absorbance values at user selected
wavelengths. Press Manage Tests, press Create Test, and the Test Definition window
will display.
Initial Test Definition display
3.5.1.1 Filter Selection
To edit the Primary or Differential Filter wavelength fields, use the arrow keys to
advance to the desired field, and then press the Edit key. The Select Filters screen
will display the available filter options; make a selection and press the Select button.
Primary Filter display
Differential Filter display
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
29
3.5.1.2 Offset Absorbance
If Differential Filter is set to "None", then the Offset Absorbance field is displayed.
Test Definition – Offset Absorbance field displayed
The user should determine the value of the Offset Absorbance. This value will be
subtracted from the absorbance readings to correct for the meniscus effect for the
test that is being run. Select the Offset Absorbance field and enter the userdetermined offset absorbance value. Press the Save button to save the settings.
Offset Absorbance feature is only needed when both of these conditions occur:
o
Monochromatic reading
o
No blank well
It is a mathematical blank to correct for the differential between air and liquid with a
meniscus.
To determine the user-entered value, pipette a volume of wash buffer into a well that
is equal to the total volume in test wells at the read time.
For example, if the well has 100µl of Substrate Part A per 100 µl of Substrate Part B,
plus 50µl of stop solution, the total volume in the test wells at the read time is 250µl.
Therefore read the absorbance of 250µl of colorless wash buffer to determine the
correct value for the Offset Absorbance. Typical values are from .250 to .400A.
30 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.5.1.3 Blank
The Test Definition Blank screen will display when the Blank field is selected. This
screen allows for the Blank setting to be enabled (Yes or No); and for inputting the
number of Replicates. Press the Done button when editing is completed.
Test Definition Blank display
3.5.1.4 Lamp Saver
Twenty minutes after reading the last sample, the Lamp Idle Timeout feature will
automatically turn off the lamp. 1200 seconds (twenty minutes) is the default setting.
The Minimum Lamp Warmup will occur (45 seconds is the default setting), and then
operation will resume where it left off. Refer to Settings to access the Lamp Control
feature.
All of the other modes function the same way but use the measured
absorbance to calculate final results.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
31
3.5.2 Factor Mode
The Factor Mode Test Definition first page will display the available options: Name, Mode
(Factor), Primary Filter, Differential Filter, and Blank. Enter each as done in Absorbance
Mode Section 3.5.1.
After pressing the advance arrows ‘>>’ in the first screen, the Test Definition second
page will display and allow editing of Factor and Controls. Use the arrow keys to
advance through the options; press the Edit key to change the values displayed on the
screen.
NOTE: The Factor comes from the assay package insert or from a previous assay in the
Single Standard Mode.
Factor Mode Test Definition – 2nd Page
Selecting any of the Control fields will open a Test Definition Control screen with options
to enable the Control (Yes or No), input the number of Replicates, Name the Control,
input a Low and a High Range, set the Action to Take (Warn, Continue or End Test),
input a Lot Number, and input an Expiration Date. Press the Done button when finished
with editing fields.
o
When Controls are read in duplicate, the mean of the duplicates can be
calculated and that one value used, or, each Control can be required to fall within
the ranges individually. “Check Mean ?” will display once duplicate Controls are
selected.
Test Definition Control screen
32 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Factor Mode (Continued)
The Factor Mode Test Definition Final Page will display and allow editing of Interpretation
Mode, Units, Decimals, and number of Sample Replicates. Selecting the Units field on
the Factor Mode Test Definition – Final Page will display a list of seven measurement
designations including a customizable selection, which are stored for labeling the
concentration column (see Section 3.4.3 Unit of Measurement Codes). Use the arrow
keys to advance through the options and press the Edit key to change the values of the
selections on the screen. Press Save to save the selections.
Factor Mode Test Definition - Final Page
Selecting the Interpretation Mode field will open the Test Definition Interpretation screen
for editing the Interpretation controls. Use the arrow keys to advance through the options,
press the Edit key to change the value of the selection.
Editing the Interpretation Pos/Neg field will display the Interpretation Normal option for
inputting Normal Range Low, Normal Range High, Valid Range Low, or Valid Range
High.
NOTE: Double check
that the interpretation
information you entered
is correct.
Factor Mode Test Definition –Pos/Neg Interpretation
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
33
Factor Mode (Continued)
Factor Mode Test Definition –Normal Interpretation
Press the Done button when editing is completed.
3.5.3 Single Standard Mode
To operate in Single Standard Mode, select Manage Tests and then select Create Test.
The Test Definition screen will display. Select the mode field and the Select Mode screen
will display the list of modes. Use the arrow keys to scroll through the mode selections to
the desired selection and press Select, or use the stylus to highlight Single Standard.
The Single Standard Mode Test Definition first page will display the available options:
Name, Mode (Single Standard), Primary Filter, Differential Filter, and Blank. These
operate the same as in the Absorbance Mode except that the Blank is required, therefore
‘YES’ will automatically appear in the Blank enabled field.
Single Standard Mode Test Definition – 1st Page
34 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Single Standard Mode (Continued)
The Single Standard Mode Test Definition second page will display and allow editing of
Standard Concentration, Controls, and number of Standard Replicates. Use the arrow
keys to advance through the options and press the Edit key to change the values of the
selections on the screen.
Single Standard Mode Test Definition –2nd Page
Selecting any of the Control fields will open a Test Definition Control screen
with options to enable the Control (Yes or No), input the number of Replicates,
Name the Control, input a Low and a High Range, set the Action to Take
(Warn, Continue or End Test), input a Lot Number, and input an Expiration
Date. Press the Done button when finished with editing fields.
o
When Controls are read in duplicate, the mean of the duplicates can be
calculated and that one value used, or, each Control can be required to
fall within the ranges individually. “Check Mean ?” will display once
duplicate Controls are selected.
Test Definition Control screen
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
35
Single Standard Mode (Continued)
The Single Standard Mode Test Definition Final page will display and allow editing of
Interpretation Mode, Units, Decimals, and number of Sample Replicates. Selecting the
Units field on the test definition Final page will display a list of seven measurement
designations including a customizable selection, which are stored for labeling the
concentration column (see Section 3.4.3 Unit of Measurement Codes). Use the arrow
keys to advance through the options; press the Edit button to change the values of the
selections on the screen. Press Save to save the selections.
Single Standard Mode Test Definition - Final Page
Selecting the Interpretation Mode field will open the Test Definition Interpretation screen
for editing Interpretation controls. Use the arrow keys to advance through the options,
press the Edit key to change the value of the selection. Press the Done button when
editing is complete.
NOTE: Double check
that the interpretation
information you entered
is correct.
Single Standard Mode Test Definition - Interpretation
36 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.5.4 Multi-Point Modes
The NEOGEN® Stat Fax® 4700 offers three varieties of multi-point modes: Point-toPoint, Regression and Cubic spline.
In Point to Point and Cubic Spline modes, the standard concentrations must be read in
either increasing or decreasing order.
All of the Test Definition screens, use of Controls, and items related to Interpretations
work the same for each type of multi-point.
Use the arrow keys to scroll through the mode selections to the desired selection and
press Select or use the stylus to highlight .
The Test Definition first page displays the available options: Name, Mode, Primary Filter,
Differential Filter, and Blank.
The Test Definition second page will display and allow editing of % Absorbance,
Standards, number of Standard Replicates, selection of Axes, and to enable the use
of Controls. Use the arrow keys to advance through the options and press the Edit
button to change the values of the selections on the screen. Note: The number of
Standards must be between two and eight for Point-to-Point and Regression. Cubic
Spline mode requires at least three Standards.
Test Definition –2nd Page
% Absorbance assigns a value of 100% to the first Standard, which must be the
Standard with the greatest absorbance. Then the % absorbance relative to the first
Standard is calculated for each Standard and Sample, in addition to a concentration
value. The %Abs value is displayed in the Interpretation field of the report. This
mode does not support ln, logit or log scales for the absorbance axis.
Upon recall of a % Absorbance mode test, the user will be asked whether to reread
the highest value Standard.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
37
Multi-Point Modes (Continued)
On the Test Definition Standards screen, the number of and concentration value of
Standards are entered.
When Duplicate Standards are used, the curve may be edited by deleting one of a
pair of Standards. (Minimum 2 for Point-to-Point and Regression; minimum 3 for
Cubic Spline). Once the curve has been read, before Accept or Print, select Edit.
Using the stylus, select the duplicate standard to be deleted. As shown below, they
are labeled as Dupl I and Dupl II.
An “X” will appear
in the location on
the display that is
to be deleted. The
curve will be
recalculated and
the options to
Accept, Edit, Print
or Quit will be
available.
Example of editing a curve with duplicate Standards
38 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Multi-Point Modes (Continued)
Axes selections include linear-linear, ln (= natural log)-linear, linear-ln, or ln-ln
calculations. A logit-log calculation is also available. Absorbance, or ln of (1000 *
absorbance), is always on the “Y” axis. Concentration, or ln of concentration, is always
on the “X” axis.
Calculation
Explanation
“Y= ABS, X=CONC”
Both the absorbance data (y) and the
concentration data (x) are linear.
“Y=Ln (1000*ABS), X=Conc”
The natural log of the absorbance is plotted
against the concentration. The absorbance
values are multiplied by 1000 before taking
the logs.
“Y=ABS, X=Ln(Conc)”
Absorbance is plotted against the natural log
of the concentration.
“Y= Ln (1000*ABS), X=Ln(Conc)”
The natural log of the absorbance is plotted
against the natural log of the concentration.
“Y= Logit(Abs), X= Log(Conc)”
Select to calculate unknowns using the
equation: Abs Logit = Ln [(sample/0 cal) / 1(sample/0 cal)]
Refer carefully to the Reagent Kit product insert for correct selection.
NOTE: Remember log of 0 is undefined so do not set concentration to 0 (zero) on a
logarithmic scale.
For Point to Point and Regression modes, number of standards can be between two and
eight.
Cubic Spline requires at least three standards.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
39
Multi-Point Modes (Continued)
The Log / Logit option is available under the Point to Point Mode, Regression Mode,
and the Cubic Spline Mode. Concentrations must be greater than zero in this axes mode.
The program automatically increases the number of Standards by one.
Log / logit
option –
the program
automatically
increases the
number of
Standards by
one.
Select Axes screen
When using a non-linear axis:

If absorbance of a Sample is > than the highest calibrator's absorbance, “ ****** “
is reported for the Concentration

Standards must be read in descending absorbance order

If no Standard programmed has a 0.0 concentration assigned to it, an additional
Standard is forced, and the concentration for that Standard is replaced with a
series of stars [ **** ]
Selecting any of the Control fields will open a Test Definition Control screen with
options to enable the Control (Yes or No), input the number of Replicates, Name the
Control, input a Low and a High Range, set the Action to Take (Warn, Continue or End
Test), input a Lot Number, and input an Expiration Date. Press the Done button when
finished with editing fields.
When Controls are read in duplicate, the mean of the duplicates can be calculated and
that one value used, or, each Control can be required to fall within the ranges
individually. “Check Mean ?” will display once duplicate Controls are selected.
40 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Multi-Point Modes (Continued)
Test Definition Control screen
The Test Definition Final page will display Interpretation Mode, Units, Decimals, and
number of Sample Replicates.
Test Definition – Final Page
Selecting the Units field will display a list of seven measurement designations including a
customizable selection, which are stored for labeling the concentration column (see
Section 3.4.3 Unit of Measurement Codes).
Selecting the Interpretation Mode field will open the Test Definition Interpretation screen
for editing the Interpretation controls. Use the arrow keys to advance through the options,
press the Edit key to change the value of the selection.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
41
Multi-Point Modes (Continued)
NOTE: Double check
that the interpretation
information you entered
is correct.
Test Definition –Pos/Neg Interpretation
Editing the Interpretation Pos/Neg field will display the Interpretation Normal option for
inputting Normal Range Low, Normal Range High, Valid Range Low, or Valid Range
High.
Press the Done button when editing is completed.
Test Definition – Normal Interpretation
42 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
3.5.5 Cut Off Mode
Many qualitative assays interpret results based on a cut off absorbance such that a
sample absorbance greater than the cut off absorbance is interpreted as positive (or
negative) and samples with absorbance less than the cut off be interpreted as negative
(or positive). Companies that manufacture these assays use a variety of means of
obtaining (reading or calculating) the essential cut off point. The reader employs a
universal equation that can be adapted to fit most commercially available kits by inserting
user-entered factors ‘X’ and/or ‘Y’ as well as a coefficient ‘F’. The equation is (X)(mNC) +
(Y)(mPC)+F = COV (Cut Off Value).
In the Cut Off Mode, ‘X ‘is multiplied by the mean of the negative controls. ‘Y’ is
multiplied by the mean of the positive controls, and each of these two numbers is added
to ‘F’ to arrive at the Cut Off Value (COV). The ‘X’, ‘Y’ and ‘F’ factors can be positive or
negative numbers, one or zero, or decimals such as 0.5 to fit the test requirements
provided in the Kit package insert or as instructed by the Kit manufacturer. In this mode
be careful to enter all QC parameters completely and correctly. They are usually
provided as Absorbance limits.
To operate in Cut Off Mode, select Manage Tests and then select Create Test. The Test
Definition screen will display. Select the Mode field and the Select Mode screen will
display the list of modes. Use the arrow keys to scroll through the mode selections to the
desired selection and press Select or use the stylus to highlight Cut Off.
Exit
Available Modes
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
43
Cut Off Mode (Continued)
The Cut Off Mode Test Definition first page displays the available options: Name, Mode
(Cut Off), Primary Filter, Differential Filter, and Blank. Enter wavelength and blank as
shown in Section 3.5.1 Absorbance Mode.
Sample of Cut Off Mode Test Definition 1st page
The Test Definition Cutoff second page allows editing of Cut Off Value (COV), values of
X, Y, and F; Negative Control; Positive Control; and QC Criteria.
Sample of Cut Off Mode Test Definition 2nd page
Selecting any of the Control fields will open a Test Definition Control screen
with options to enable the Control (Yes or No), input the number of Replicates,
Name the Control, input a Low and a High Range, set the Action to Take
(Warn, Continue or End Test), input a Lot Number, and input an Expiration
Date. Press the Done button when finished editing fields.
44 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Cut Off Mode (Continued)
Test Definition Control Screen
When Cutoff Controls are read in multiples, the mean of the replicates can
be calculated and that value used, or each Control can be required to fall
within the ranges set. “Check Mean” will display once replicate Controls are
selected.
Selecting the ‘COV =’ field will open the Select COV Formula screen. In addition to the
universal equation, there are three optional formulas.
Where COV is the Cutoff Absorbance Value, mNC is the mean of the Negative Controls;
mPC is the mean of the Positive Controls. X, Y, and F are user-entered coefficients that
can have any positive or negative numerical value including zero and 1.
Highlight the desired formula from the list and press the Select button.
See explanation
of choices in the
table below.
COV Formula options
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
45
Cut Off Mode (Continued)
COV Formula
X*mean(NC) + Y*mean(PC) + F
Explanation
 Universal equation, used to calculate the
cutoff value based upon readings or
positive and/or negative controls.
 In this equation, NC and PC are used to
determine the COV (cutoff value).
 Enter the variables provided in the package
insert.
 This will calculate the mean of the
positives and the mean of the negatives.
X * mean(NC) + F
 Use this equation if only a negative control
is used to determine the COV.
Y * mean(PC) +F
 Use this equation if only a positive control
is used to determine the COV.
 For interpretation, you may chose either
the Regular Cut Off Mode (positive >=
cutoff, negative < cutoff), or the Reverse
Cut Off Mode. With the Reverse Cut Off
option, the Samples with values lower than
the cut off are labeled as “positive”. If you
choose this option, be careful to follow the
< and > signs in the prompts for entering
cut offs and ranges.
46 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Cut Off Mode (Continued)
COV Formula
X*mean(CC) + F
Explanation
 COV = cut off value for positive or
negative interpretation
 X = a variable provided in the Assay
Kit package insert. NOTE: X will
equal 1 if no value of variable
provided.
 mCC = the calculated mean of the
absorbance values of the cutoff
controls when test is run
 Blanking is optional, dependent
upon the Assay Kit
 F = factor added to the mCC. If
needed,
This value is provided in the Assay Kit
package insert. If none provided, use zero
for the value of F.
Positive and/or negative controls may be
used for QC criteria
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
47
Cut Off Mode (Continued)
Selecting the QC Criteria field opens the Test Definition Cutoff Quality screen:
(mPC / mNC):
Enter the lowest
acceptable ratio
for the mean of
the positive
controls to the
mean of the
negative controls
(mPC – mNC):
Enter the lowest
acceptable
difference for the
mean absorbance
of the positive
controls minus the
mean absorbance
of the negative
controls.
Note: ‘Reversed’ field is ‘YES’
when low absorbances are
positive and high absorbances
are negative, ‘Blank’ is not
subtracted.
Cutoff Mode – Quality Criteria screen
If the Reversed field reads ‘YES’ the samples with values lower than the cutoff are
labeled as positive. In addition, the Blank is not subtracted in the Reverse Cutoff option.
Pay close attention to “<” less than and “>” greater than symbols when entering control
limits in this case.
The Cut Off Mode Test Definition final page displays Interpretation Mode, Units,
Decimals, and number of Sample Replicates.
Cut Off Mode Test Definition - Final page
48 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Cut Off Mode (Continued)
Use the arrow keys to advance through the options and press the Edit key to change the
values of the selections on the screen. Press Save to save the edits made.
Selecting the Interpretation Mode field will open the Test Definition Interpretation screen
for editing Interpretation Controls. Use the arrow keys to scroll through the options, press
the Edit key to change the value of the selection.
Press the Done button when finished editing.
NOTE: Double check that
the COV calculations and
interpretation information
you entered is correct.
Cut Off Mode Test Definition - Interpretation
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
49
4. CLEANING AND MAINTENANCE
4.1 Cleaning
4.1.1 Exterior
CAUTION: Solvents such as acetone or thinner will damage the instrument!
Use only water and recommended cleaners! Avoid abrasive cleaners. The
display area is liquid-resistant, but is easily scratched.
The exterior of the instrument may be cleaned with a soft cloth using plain water. If
needed, a mild all-purpose (nonabrasive) cleaner may be used. A 1.5% solution of
chlorine bleach (5.25% Sodium Hypochlorite) or 70% isopropyl alcohol may be used as a
disinfectant. Take special care not to spill any liquid into the read well.
4.2 Maintenance
4.2.1 Calibration and Linearity
Each instrument is calibrated during manufacturing using standards that are traceable to
the National Institute for Standards and Testing (NIST), and is tested to verify its linearity
to 3A. This preset calibration is very stable. Absolute calibration can be verified with the
use of NIST filters, or by periodic comparison to a reference instrument that is known to
be calibrated to NIST filters.
Absolute calibration can be verified using a specifically designed product called DRI-DYE®
Check Strips, available from your instrument supplier.
The best way to assure quality instrument performance is to include a sufficient number of
controls in each assay to cover the entire operational range.
In vertical photometry, the fill volume and degree of meniscus determine the pathlength;
and absorbance is proportional to pathlength. Since lab test results are typically based
upon standards rather than upon absolute absorbances, the linearity of the instrument is
the more critical indicator of instrument performance.
A reduction in linearity may be indicative of filter deterioration. In this event, filter
replacement is required for continued reliable operation. A monthly verification of
instrument linearity is generally required to comply with regulatory agencies.
DRI-DYE® Check Strips can also be used to verify linearity.
Alternately, the degree of linearity can be checked by reading a 1:2 serial dilution of a
material having a peak absorbance at or near one of the wavelengths, and observing a
1:2 ratio in the absorbance readings.
If you prepare dilutions to verify linearity, uniform pipetting (pathlength) and minimal
transfer error are important, since the tolerance limits which you will establish must take
into consideration the sources of error that are not due to the instrument.
Pipetting errors can be identified by comparing duplicate readings. The instrument should
give the expected value ± (1% of the expected value + .01A).
For example, if the 1/4 dilution reads .520 A, then you can expect your 1/2 dilution sample
to read twice as much or 1.04 A ± [(1% of 1.04) +0.01 A], which is ± .02.
50 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Calibration and Linearity (Continued)
A good working instrument would then give a result between 1.02 A and 1.06 A. You will
know that your filters are deteriorating when darkly colored solutions consistently read
lower than expected.
Since the procedures for verifying the calibration and linearity of a vertical photometer are
quite cumbersome and not conducive to stringent criteria, the best way to assure quality
instrument performance is with DRI-DYE® Check Strips.
4.2.2 Storage
The instrument may be stored under the following recommended environmental conditions:

Temperature: 10 to 50°C

Humidity: Less than 80% relative humidity, non-condensing.
Store the instrument using original packaging if possible.
Perform the following steps before storing:
 Set the power switch to OFF (O) and remove the power cord.
 Place the instrument in the original packaging material.
 When returning the instrument to service from storage, it is recommended that
functional tests be performed as if setting up the instrument for the first time.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
51
5. TROUBLESHOOTING
5.1 Flags Messages
Flags are displayed to alert the operator when certain limits are approached. After displaying
the warning the instrument will continue to function normally.
Flag Message
Explanation
“>10**6”
Appears in Concentration Column when Concentration
is greater than 10**6 (column only holds six characters
maximum)
“Assay Cannot Be
Edited!”
User has tried to edit an OEM preprogrammed test
that has flag set to prevent it from being edited.
“Can not match test
wavelength”
While printing the test header, if the index of the
primary or differential filter that was set up in the assay
does not match what is set up in the instrument the
message displays “Can not match test wavelength
with filters currently installed on device!”.
“Can not match test
wavelength with
filters currently
installed on device!”
Pop up message that appears when running a test;
occurs if the device cannot find filters for the
wavelengths set in the test definition.
“Carrier out of
position. Set Carrier.
Press OK to
continue”.
Instrument detecting no carrier while running a test.
“Default settings
loaded.
CALIBRATION
LOST!”
If factory unit parameters do not load, then the unit
parameters are reinitialized.
“Do not select the
same filter for
primary and
differential”
Pop up message that appears during test definition if
the same wavelengths are entered for the Primary and
the Differential Filters.
“Error Saving Assay
Data!”
The Save Assay function has returned an error.
“FTDIInit() failed
Press OK to proceed with recovery
“Index Tests Cannot
Be Edited”
User has tried to edit an OEM preprogrammed index
test (i.e. Cardiac Risk, TIBC).
52 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Troubleshooting (Continued)
Flag Message
“Invalid Curve!”
Explanation
Pop up message that appears when absorbances
read for standards are not either steadily increasing or
decreasing. “Curve Invalid” is output to the printer.
Printed in Multi-Point Modes only: Point-to-Point,
Regression, and Cubic Spline.
If duplicate standards are used, the user has the
option to edit the curve if there are enough points
(minimum 3 for Point-to-Point and Regression;
minimum 4 for Cubic Spline).
“Lamp/Filter Volts
Low”
Lamp seems dim or is blocked
“Must be between XX
and XX”
Pop up message that is a number pad range error;
occurs when user makes an entry on the number pad
that is outside of the range for that value.
“Not enough wells
selected to run assay
as defined”
When user changes the total number of samples while
running a test and the user did not map enough wells
to run what is defined in the test.
“Not enough wells
selected to run #
sample replicates
defined”
Due to the number wells available, the test could not
be run with the number of sample replicates defined.
“Pos must be greater
than or equal to Neg!”
Pop up message that appears during test definition
when the user is defining positive/negative
interpretations. A value was entered for both the
positive and negative, but the positive value is less
than the negative value entered.
“Set Carrier to
Position 1(or A).
Press OK to
continue”
Displays if the carrier is not in the first position.
“Settings from last
Calibration used”
During startup, if there is an error loading the unit
parameters and instrument successfully loads the
factory unit parameters.
“Updating internal
firmware”
If the system finds an old version of FTDI firmware,
flag message displays and it is updated automatically.
“Wait for Lamp
Warmup”
Pop up message that appears when running a test
and the lamp on the photometer is not ready yet.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
53
Troubleshooting (Continued)
Flag Message
Explanation
“When reversed, pos
must be less than
neg!”
Pop up message that appears during test definition
when the user is defining Positive/Negative
interpretations; a value was entered for both the
positive and negative and the reverse was selected,
but the positive value is greater than the negative
value entered.
“Wrong date format
(mm.yyyy)!”
Pop up message that appears when the expiration
date for a control is not entered in the correct format
during test definition.
Messages that appear in the Interpretation Column:
“******”
Prints when a sample exceeds the absorbance of the
reference well in a log-logit test. Curve Axes is
Y=Logit(Abs), X=Log(Conc) and Absorbance
>reference(zero) absorbance value.
“******”
Prints in Interpretation column whenever a curve axis
mode with a log tries to log a zero, or a negative
number, or an invalid curve.
“******”
Prints in Concentration column whenever an error
occurs while calculating the concentration.
“>3”
Prints in ABS column if a read absorbance is above
the maximum readable absorbance for the device.
“EQ”
If both POS and NEG values are set and are different,
sample is <POS value and >NEG value (or if reversed,
sample is >POS value and <NEG value).
“LOW”, “HIGH”
Control is outside low/high normal concentration range
values.
“NEG”
Sample is <NEG value (or if reversed, sample is
>NEG value).
“OUT”
Blank is outside min/max range or sample is outside
low/high valid range values. Also appears during
Cubic Spline Mode when the sample is outside of the
range of valid standards.
“POS”
Sample is >= POS value (or if reversed, sample is
<=POS value.
“S#=x.xxx Deleted”
Standard was deleted. Only allowed when duplicate
standards are run. One Standard of each pair can be
deleted. Example: Using edited curve –
S1=0.500 deleted
S2=1.000 deleted
54 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Troubleshooting (Continued)
5.2 Error Messages
Error Messages are displayed when the instrument fails to operate correctly or when an
invalid range is entered. They are intended to help the user locate the problem. Some error
messages appear as dialog boxes that require action in order to clear; others appear on the
display screen and appear on the printout.
Error Message
Explanation
“Do not select the
same filter for
primary and
differential”
Appears on Page 1 of Test Definition when the same
wavelengths are entered for the Primary and the
Differential filters.
“Mechanism Error”
Appears during the initialization of a carrier, if there is
an error moving carrier or selecting filters.
“%Absorbance Mode
should not be used
with negative
absorbances!”
This message will display if the user is in
%Absorbance Mode and a standard is read which has
a negative absorbance.
Log Logit Mode error message:
“Invalid Curve – Log
Error”
An error in the creation of a curve or readings of a
negative absorbance will result in this message,
followed by Test Ended.
5.3 Hardware Messages
Should any of the EEPROM error messages below appear, promptly contact technical
support.
Type
EEPROM
error
Message
Test Data
Corrupted!
Explanation
Checksum for Assay Data (including
calibration data) does not match what was
stored for that Assay.
Contact technical support.
Test Definition
Corrupted!
Checksum for Test Definition does not match
what was stored for that Assay.
Contact technical support.
Error Saving
Assay Data!
Unable to store Assay information into
EEPROM.
Contact technical support.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
55
6. REFERENCES
1. Engineering data supplied by Awareness Technology, Inc. Palm City, Florida (1987 to
current date)
2. Data on DRI-DYE® Check Strips, provided by Awareness Technology, Inc. Palm City,
Florida (1989 to current date)
7. OPTIONAL ACCESSORIES
7.1 Thermal Printer Paper
Reorder replacement Thermal Printer Paper Roll P/N 150006. NOTE: The printer
manufacturer strongly recommends the use of thermal paper part number 150006 to ensure
the best print quality and the effective operation of the print head. Contact your dealer for
replacement rolls.
7.2 Dri-Dye® Check Strips
DRI-DYE
®
CHECK STRIPS

Dri-Dye® Check Strips offer an easy method of verifying proper calibration and linearity
on a routine basis. The charts provided allow simple interpretation of results. If a strip
reader can repeatedly obtain acceptable results with this kit, then the instrument
demonstrates linearity, calibration, filter integrity, repeatability, and lack of stray light.

Proper use of the Dri-Dye® Check Strips, along with good record keeping, will constitute
compliance with most quality control and licensing agency requirements for instrument
performance verification.

Dri-Dye® Check Strips allow the instrument owner to perform testing that is very similar to
the Q.A./Q.C. testing initially done by the instrument manufacturer. Dye absorbance
values are determined by a reference instrument that is routinely calibrated with
materials traceable to the National Institute of Standards and Technology (NIST, formerly
NBS). Dyes are then precisely dispensed into the wells, dried, and packaged.

Using pre-dispensed dyes reduces the degree of pipetting precision required to
reconstitute the check set strips. Since the readers look through the samples from top to
bottom, volume is proportional to pathlength, and hence, to absorbance. If a well is
reconstituted with too much water, the decrease in concentration will be corrected by the
increase in pathlength. Simply stated, the system is self correcting for random pipetting
errors up to 10%.

DRI-DYE® Check Strips can be ordered from your dealer.
The charts provided with these kits are designed to replace the normal calculations with easy
to visualize, built-in acceptance ranges. Kits are available for the following wavelengths:
o
DRI-DYE® Check Strips-405 - for 405nm
o
DRI-DYE® Check Strips-450 - for 450nm
o
DRI-DYE® Check Strips-492 - for 492nm
56 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
8. CONTACT INFORMATION
In the unlikely event that a problem occurs with the instrument, please consult your dealer
first.
Dealer:
Important: When contacting us, please have the Model and Serial Number of the instrument
in question. Have a description of the problem with as much detail as possible. Save any
relevant printouts and send or e-mail us the information.
NEOGEN®Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
57
9. APPENDIX A - SF_CAPTURE
SF_Capture is a software program for use with this reader. Contact your dealer for a copy of
the program.
Install the program by double clicking on the setup.exe file found in the SF_Capture folder.
The installation process is the same as other software – follow the prompts until it is done.
Power on the instrument, connect the serial port to USB cable from the reader to the PC.
Verify that External Export has been switched to ON from the instrument's Settings menu
NOTE: An installation of drivers will need to be done the first time SF_Capture is
installed on a PC.
Once the cable connection is made between the reader and the PC, Windows® will pop up a
message box stating that the VNC1-A As Slave is not installed and that drivers will need to
be found.
Correct response is to let Windows® look for the appropriate driver this one time.
First, it will search for and install USB Serial Converter driver(s).
The same window will open again. Windows® will search for and install USB Serial Port
driver(s).
The cables can be connected and the instrument's power up can be done in any sequence.
If the instrument is OFF, but SF_Capture is open and the cable is connected to the
instrument, select ReConnect.
Select Settings. This will open a dialog box where you select the instrument type and the
COM port connected to on the PC side.
Data is exported to Microsoft® Excel®, to Notepad and to a Log file each time QUIT/Test
Ended occurs.The default location for these files is C:\4700. This can be changed from the
default by using the Browse option for the Device Root Folder.
58 NEOGEN® Stat Fax® 4700 Operator’s Manual Doc. 4701 Rev. A
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertising