Zeiss Axiovert 200 Brochure

Zeiss Axiovert 200 Brochure
AXIOBR_E.qxd
27.03.2001
14:14 Uhr
Seite 1
Microscopy from Carl Zeiss
System Overview
Axiovert
200 M
M
Axiovert 200
Axiovert 200
The New Standard
in Inverted Microscopy
Axiovert 200
G
er
m
an
y
2-axes control panel
457433-0000-000
only together
with LSM 510:
up
X
Y
Motor control MCU 28
457428-0000-000
MCU
PO
28
ER
WE
R
Dust cover
459330-0000-000
AxioCam and Light Trap are trademarks of Carl Zeiss.
HRZ 200 fine focusing stage
000000-1013-186
wn
RO
RU
34
R
N
56
78
CAN bus cable 2.5 m
457411-9011-000
Scanning stage DC 120x100
000000-1113-509
Path-deflecting mirror mot, Subbus
000000-1095-490
LD condenser 0.55 mot H, Ph 1, Ph 2, Ph 3, DIC, DIC
000000-1005-848
R
e
v
Excitation filter wheel
(8 positions) MOT
for 25 mm filters
000000-1008-106
98
Axiovert, AxioVision, LSM 5 PASCAL and Optovar is a registered trademark of Carl Zeiss.
R
do
Axiovert 200
Unique Down to the Last Detail
Live cell imaging, micromanipulation on living
cells – never before have such rigorous demands
been placed on the stability, flexibility, reliability
and operating comfort of microscopes. And
never before has there been a microscope capable of meeting these demands with such ease
and elegance. Its name: Axiovert 200, the state
of the art in inverted microscopy from Zeiss.
Axiovert 200 is based on the extraordinary
expertise and experience of a company which
has been setting international standards in
inverted microscopy since 1924. A company that
developed the first microscope with infinity
optics. But above all: this high-end microscope
surpasses earlier models in every crucial detail:
the outstanding Zeiss optics with the wide range
95
of ICS objectives, with innovative condensers and
415
642
Axiovert 200 M basic stand (*)
000000-1005-820
AxioVision Control Software
000000-1068-563
RS 233 cable
457411-9a012-000
REF
FO
CU
LEC
R
365
519
scope of sophisticated documentation and adaptation options, exceptional stability and unique
170
TO
S
297
Control PC-board with interface
000000-1015-381
(contained in basic stand)
E
ergonomic design.
187
O
RO
TIV
195
398
ZE
C
BJE
optimized fluorescence as well as with an unusual
Ax
io
Ca
m
And Axiovert 200 M gives you the freedom to
Adapter Video V200 C 2/3" 0.63x
at frontport
000000-1071-171
High Resolution Microscopy Camera AxioCam b/w
000000-0412-311
High Resolution Microscopy Colour Camera AxioCam Colour
000000-0412-312
468
150
590
294
Tube lens 1.0 for LSM
000000-1071-169
automate many routine procedures – so you save
time with no loss in reliablity.
265
As a matter of fact, Axiovert 200 is much more
Weight: Axiovert 200
ca. 24 kg (depending on model)
Axiovert 200 M ca. 40 kg incl. fluorescence
5-position mot. reflector turret, empty
000000-1064-365
5-position reflector turret, empty
000000-1064-364
than the sum of its benefits. And why? Because
it gives you a powerful basis to carry out your
work in all life science applications more precisely, more rapidly and more successfully than
For further details, please contact:
Beam path switching mot
000000-1069-229
33
LSM-adapter SP left and sideport 60 right, 3 switching positaions
000000-1071-164
LSM adapter BP and sideport 60 left, 3 switching positions
000000-1071-165
LSM adapter BP and FCS adapter SP left, 3 switching positions
000000-1071-166
Safety installations LSM 510/PASCAL
000000-1071-174
Beam path switching mot LSM
000000-1069-230
(*) If there are no special motorized components, all components of the manual stand
can also be used at the motorized stand.
Carl Zeiss
Light Microscopy
P.O.B. 40 41
37030 Göttingen
GERMANY
Phone : ++49 5 51 50 60 660
Telefax: ++49 5 51 50 60 464
E-Mail: [email protected]
www.zeiss.de/micro
Subject to change.
Printed on environment-friendly paper,
bleached without the use of chlorine.
before. In other words: it gives you the basis that
40-085 e/03.01
ensures you’re in the lead. And that’s exactly
where you need to be in science today.
If you need the best,
there is simply no alterantive
AXIOBR_E.qxd
27.03.2001
14:14 Uhr
Seite 1
Microscopy from Carl Zeiss
System Overview
Axiovert
200 M
M
Axiovert 200
Axiovert 200
The New Standard
in Inverted Microscopy
Axiovert 200
G
er
m
an
y
2-axes control panel
457433-0000-000
only together
with LSM 510:
up
X
Y
Motor control MCU 28
457428-0000-000
MCU
PO
28
ER
WE
R
Dust cover
459330-0000-000
AxioCam and Light Trap are trademarks of Carl Zeiss.
HRZ 200 fine focusing stage
000000-1013-186
wn
RO
RU
34
R
N
56
78
CAN bus cable 2.5 m
457411-9011-000
Scanning stage DC 120x100
000000-1113-509
Path-deflecting mirror mot, Subbus
000000-1095-490
LD condenser 0.55 mot H, Ph 1, Ph 2, Ph 3, DIC, DIC
000000-1005-848
R
e
v
Excitation filter wheel
(8 positions) MOT
for 25 mm filters
000000-1008-106
98
Axiovert, AxioVision, LSM 5 PASCAL and Optovar is a registered trademark of Carl Zeiss.
R
do
Axiovert 200
Unique Down to the Last Detail
Live cell imaging, micromanipulation on living
cells – never before have such rigorous demands
been placed on the stability, flexibility, reliability
and operating comfort of microscopes. And
never before has there been a microscope capable of meeting these demands with such ease
and elegance. Its name: Axiovert 200, the state
of the art in inverted microscopy from Zeiss.
Axiovert 200 is based on the extraordinary
expertise and experience of a company which
has been setting international standards in
inverted microscopy since 1924. A company that
developed the first microscope with infinity
optics. But above all: this high-end microscope
surpasses earlier models in every crucial detail:
the outstanding Zeiss optics with the wide range
95
of ICS objectives, with innovative condensers and
415
642
Axiovert 200 M basic stand (*)
000000-1005-820
AxioVision Control Software
000000-1068-563
RS 233 cable
457411-9a012-000
REF
FO
CU
LEC
R
365
519
scope of sophisticated documentation and adaptation options, exceptional stability and unique
170
TO
S
297
Control PC-board with interface
000000-1015-381
(contained in basic stand)
E
ergonomic design.
187
O
RO
TIV
195
398
ZE
C
BJE
optimized fluorescence as well as with an unusual
Ax
io
Ca
m
And Axiovert 200 M gives you the freedom to
Adapter Video V200 C 2/3" 0.63x
at frontport
000000-1071-171
High Resolution Microscopy Camera AxioCam b/w
000000-0412-311
High Resolution Microscopy Colour Camera AxioCam Colour
000000-0412-312
468
150
590
294
Tube lens 1.0 for LSM
000000-1071-169
automate many routine procedures – so you save
time with no loss in reliablity.
265
As a matter of fact, Axiovert 200 is much more
Weight: Axiovert 200
ca. 24 kg (depending on model)
Axiovert 200 M ca. 40 kg incl. fluorescence
5-position mot. reflector turret, empty
000000-1064-365
5-position reflector turret, empty
000000-1064-364
than the sum of its benefits. And why? Because
it gives you a powerful basis to carry out your
work in all life science applications more precisely, more rapidly and more successfully than
For further details, please contact:
Beam path switching mot
000000-1069-229
33
LSM-adapter SP left and sideport 60 right, 3 switching positaions
000000-1071-164
LSM adapter BP and sideport 60 left, 3 switching positions
000000-1071-165
LSM adapter BP and FCS adapter SP left, 3 switching positions
000000-1071-166
Safety installations LSM 510/PASCAL
000000-1071-174
Beam path switching mot LSM
000000-1069-230
(*) If there are no special motorized components, all components of the manual stand
can also be used at the motorized stand.
Carl Zeiss
Light Microscopy
P.O.B. 40 41
37030 Göttingen
GERMANY
Phone : ++49 5 51 50 60 660
Telefax: ++49 5 51 50 60 464
E-Mail: [email protected]
www.zeiss.de/micro
Subject to change.
Printed on environment-friendly paper,
bleached without the use of chlorine.
before. In other words: it gives you the basis that
40-085 e/03.01
ensures you’re in the lead. And that’s exactly
where you need to be in science today.
If you need the best,
there is simply no alterantive
AXIOBR_E.qxd
27.03.2001
14:16 Uhr
Seite 5
Milestones
100 years of tradition and innovation in inverted
microscopy – with developments that set the standards
of their time. Now a new microscope from Carl Zeiss
is continuing this success story: Axiovert 200, yet
another milestone in research and routine.
Large Metal Microscope
Stand LU
1924 Large research microscope with
the first “infinite” objectives in the world.
1934 Neophot – the microscope
with the first darkfield and Köhler
illumination.
1935 Lu stand – the first upright basic
stand that could be converted into an
inverted one.
Axiomat IDC
1968 Telaval – movable stage, upright
non-mirror image, microphotography
with automatic exposure.
1973 Axiomat IDC – the start of
inverted high-performance microscopy
with its own infinite optics.
1973 Invertoskop D –
for routine applications.
IM 35
1976 IM and IM 35 – the first inverted
microscopes for all contrasting methods,
including reflected light fluorescence.
1987 The first Axiovert generation –
worldwide the first with complete
ICS optics (Infinity Color Corrected
Optical System).
2000 Axiovert 200 – the beginning of a new era in
inverted high-end microscopy.
2
3
AXIOBR_E.qxd
27.03.2001
14:16 Uhr
Seite 5
Milestones
100 years of tradition and innovation in inverted
microscopy – with developments that set the standards
of their time. Now a new microscope from Carl Zeiss
is continuing this success story: Axiovert 200, yet
another milestone in research and routine.
Large Metal Microscope
Stand LU
1924 Large research microscope with
the first “infinite” objectives in the world.
1934 Neophot – the microscope
with the first darkfield and Köhler
illumination.
1935 Lu stand – the first upright basic
stand that could be converted into an
inverted one.
Axiomat IDC
1968 Telaval – movable stage, upright
non-mirror image, microphotography
with automatic exposure.
1973 Axiomat IDC – the start of
inverted high-performance microscopy
with its own infinite optics.
1973 Invertoskop D –
for routine applications.
IM 35
1976 IM and IM 35 – the first inverted
microscopes for all contrasting methods,
including reflected light fluorescence.
1987 The first Axiovert generation –
worldwide the first with complete
ICS optics (Infinity Color Corrected
Optical System).
2000 Axiovert 200 – the beginning of a new era in
inverted high-end microscopy.
2
3
AXIOBR_E.qxd
27.03.2001
14:16 Uhr
Seite 5
Milestones
100 years of tradition and innovation in inverted
microscopy – with developments that set the standards
of their time. Now a new microscope from Carl Zeiss
is continuing this success story: Axiovert 200, yet
another milestone in research and routine.
Large Metal Microscope
Stand LU
1924 Large research microscope with
the first “infinite” objectives in the world.
1934 Neophot – the microscope
with the first darkfield and Köhler
illumination.
1935 Lu stand – the first upright basic
stand that could be converted into an
inverted one.
Axiomat IDC
1968 Telaval – movable stage, upright
non-mirror image, microphotography
with automatic exposure.
1973 Axiomat IDC – the start of
inverted high-performance microscopy
with its own infinite optics.
1973 Invertoskop D –
for routine applications.
IM 35
1976 IM and IM 35 – the first inverted
microscopes for all contrasting methods,
including reflected light fluorescence.
1987 The first Axiovert generation –
worldwide the first with complete
ICS optics (Infinity Color Corrected
Optical System).
2000 Axiovert 200 – the beginning of a new era in
inverted high-end microscopy.
2
3
Axiovert 200 – The Optics
Limited Only by
the Laws of Physics
The Objectives :
The New Condensers:
The Observation Tubes :
Excellent in Contrast
Distinguished
Flexibility
by Distance
in Focusing
LD condensers : The extra long
More space for manipulation in
Axiovert 200 meets all demands
working distance is especially im-
microscopy: With N.A. 0.35 (WD
with three tubes:
portant in inverted microscopy in
70 mm) and N.A. 0.55 (WD 26
1. Binocular tube. The integrated
order to penetrate thick vessels as
mm), the new top LD condensers
shutter prevents ambient light
easily as possible.
offer considerably more working
from entering the stand through
Ever since Ernst Abbe and his
The LD A-Plan objectives have
distance – so important for easy
the eyepiece during low light level
famous formula, it is a known fact
already been corrected for all
handling of manipulators and rapid
image acquisition, thereby con-
that resolution is determined by
standard 1 mm chamber thickness
observation of the sample. But
tributing stray light.
the numerical aperture of the
(+/– 0.4 mm), thereby yielding
also more freedom in contrasting
2. Phototube. Features TV port
objective and the condenser. Only
results that are strong in contrast
methods. The new LD condensers
(60 mm interface) with 3 positions
when the quality and interaction
and free of aberrations.
LD – long distance – objectives and
can be used in every area of appli-
(vis/photo: 0/100, 100/0 and 50/50),
of both components are 100 %
cation, thanks to their numerous
shutters and additional focusing
perfect – as with Axiovert 200 – is
modulators for Phase contrast,
Bertrand lens for easy adjustment
image quality optimal. ICS infinity
Varel or DIC. A new and highly
of the phase rings.
interesting feature: DIC prisms can
3. Height adjustable Ergotube with
even be used with the condensers
focusing Bertrand lens, shutter and
with an N.A. of 0.35. Convincing
an ergonomically ideal 25° viewing
evidence of Axiovert 200’s impres-
angle, with 50 mm range of travel.
sive flexibility: A special adapter
Now fatigue-free work for hours on
allows you to use the high-resolu-
end is no longer a problem.
optics: limited only by the laws of
physics.
Optics to perfection.
For every method, Zeiss
provides you with a wide
range of ICS objectives
tion condensers with N.A. of 0.8
and 1.4 from the family of upright
microscopes.
The new LD condensers. Making sure that ICS optics provide full performance potential.
N.A.: 0.35 – WD 70 mm, for BF; Ph 0, 1, 2; DIC I, II; Var 1
N.A.: 0.55 – WD 26 mm, for BF; Ph 1, 2, 3; DIC I, II, III; Var 1, 2
N.A.: 0.8 – WD 7 mm, for BF; Ph 1, 2, 3; DIC III; D 0,6, 0,8
N.A.: 1.4 – oil immersion, for BF; Ph 2, 3; DIC II, III; D 1,1, 1,2
(From top to bottom)
Binocular tube
Phototube
Ergotube
4
5
Axiovert 200 – The Optics
Limited Only by
the Laws of Physics
The Objectives :
The New Condensers:
The Observation Tubes :
Excellent in Contrast
Distinguished
Flexibility
by Distance
in Focusing
LD condensers : The extra long
More space for manipulation in
Axiovert 200 meets all demands
working distance is especially im-
microscopy: With N.A. 0.35 (WD
with three tubes:
portant in inverted microscopy in
70 mm) and N.A. 0.55 (WD 26
1. Binocular tube. The integrated
order to penetrate thick vessels as
mm), the new top LD condensers
shutter prevents ambient light
easily as possible.
offer considerably more working
from entering the stand through
Ever since Ernst Abbe and his
The LD A-Plan objectives have
distance – so important for easy
the eyepiece during low light level
famous formula, it is a known fact
already been corrected for all
handling of manipulators and rapid
image acquisition, thereby con-
that resolution is determined by
standard 1 mm chamber thickness
observation of the sample. But
tributing stray light.
the numerical aperture of the
(+/– 0.4 mm), thereby yielding
also more freedom in contrasting
2. Phototube. Features TV port
objective and the condenser. Only
results that are strong in contrast
methods. The new LD condensers
(60 mm interface) with 3 positions
when the quality and interaction
and free of aberrations.
LD – long distance – objectives and
can be used in every area of appli-
(vis/photo: 0/100, 100/0 and 50/50),
of both components are 100 %
cation, thanks to their numerous
shutters and additional focusing
perfect – as with Axiovert 200 – is
modulators for Phase contrast,
Bertrand lens for easy adjustment
image quality optimal. ICS infinity
Varel or DIC. A new and highly
of the phase rings.
interesting feature: DIC prisms can
3. Height adjustable Ergotube with
even be used with the condensers
focusing Bertrand lens, shutter and
with an N.A. of 0.35. Convincing
an ergonomically ideal 25° viewing
evidence of Axiovert 200’s impres-
angle, with 50 mm range of travel.
sive flexibility: A special adapter
Now fatigue-free work for hours on
allows you to use the high-resolu-
end is no longer a problem.
optics: limited only by the laws of
physics.
Optics to perfection.
For every method, Zeiss
provides you with a wide
range of ICS objectives
tion condensers with N.A. of 0.8
and 1.4 from the family of upright
microscopes.
The new LD condensers. Making sure that ICS optics provide full performance potential.
N.A.: 0.35 – WD 70 mm, for BF; Ph 0, 1, 2; DIC I, II; Var 1
N.A.: 0.55 – WD 26 mm, for BF; Ph 1, 2, 3; DIC I, II, III; Var 1, 2
N.A.: 0.8 – WD 7 mm, for BF; Ph 1, 2, 3; DIC III; D 0,6, 0,8
N.A.: 1.4 – oil immersion, for BF; Ph 2, 3; DIC II, III; D 1,1, 1,2
(From top to bottom)
Binocular tube
Phototube
Ergotube
4
5
Axiovert 200 –
The Contrasting
Methods
Quality and versatility:
Axiovert 200 is ready for every
contrasting method. And consequently for the solution that
works the best for your application and makes the most economic sense.
A Powerful Program
Double the Benefit :
With or Without
Two Methods –
Sénarmont:
One Objective
The DIC Methods
A quick look at the range of Zeiss
For the best Differential Interfer-
objectives is enough to convince
ence Contrast (DIC) that you have
you of the benefits they offer. The
ever seen, you must have the best
Varel objectives are a combination
components. Consequently for
of phase contrast and Varel, there-
every objective there is a DIC slid-
by providing you with the unique
er, positioned in the objective tur-
possibility of imaging your speci-
ret, which matches it optimally.
mens both three-dimensionally
There is also the corresponding
and in phase contrast. Depending
prism, which is found in the con-
on the application, you decide
which method you would like –
without changing the objective.
The Zeiss Variable Relief contrast
is so versatile that it can be used
for all cells, no matter what type,
no matter how thick. Additional
Embryonic rat cells,
triple fluorescent labeling,
R. Learish, Promega
Corporation, Madison,
WI
denser turret. You have the choice
C. elegans, embryo, DIC,
Prof. Schnabel,
Techn. Universität,
Braunschweig
each other and contrast is a-
of two methods. First: DIC according to Nomarski. Here polarizer
and analyzer are at right angles to
chieved by adjusting the DIC slider.
Second : DIC with Sénarmont.
flexibility that will considerably
Instead of a DIC slider, a revolving
simplify your work.
analyzer with λ/4 plate is used to
attain contrast. The advantage of
the second method – considerably
increased accessibility – is particu-
Electrofusion of 2-cell stage
embryo, phase contrast,
K. Vintersten, S. Gray,
EMBL Heidelberg
larly evident in the case of larger,
lateral setups with cameras and
manipulators. Moreover, once the
small DIC sliders have been cen-
Normal embryo and
mutant, darkfield,
R. Adams,
EMBL Heidelberg
6
tered, they no longer have to be
adjusted.
Sénarmont analyzer
and DIC slider
7
Axiovert 200 –
The Contrasting
Methods
Quality and versatility:
Axiovert 200 is ready for every
contrasting method. And consequently for the solution that
works the best for your application and makes the most economic sense.
A Powerful Program
Double the Benefit :
With or Without
Two Methods –
Sénarmont:
One Objective
The DIC Methods
A quick look at the range of Zeiss
For the best Differential Interfer-
objectives is enough to convince
ence Contrast (DIC) that you have
you of the benefits they offer. The
ever seen, you must have the best
Varel objectives are a combination
components. Consequently for
of phase contrast and Varel, there-
every objective there is a DIC slid-
by providing you with the unique
er, positioned in the objective tur-
possibility of imaging your speci-
ret, which matches it optimally.
mens both three-dimensionally
There is also the corresponding
and in phase contrast. Depending
prism, which is found in the con-
on the application, you decide
which method you would like –
without changing the objective.
The Zeiss Variable Relief contrast
is so versatile that it can be used
for all cells, no matter what type,
no matter how thick. Additional
Embryonic rat cells,
triple fluorescent labeling,
R. Learish, Promega
Corporation, Madison,
WI
denser turret. You have the choice
C. elegans, embryo, DIC,
Prof. Schnabel,
Techn. Universität,
Braunschweig
each other and contrast is a-
of two methods. First: DIC according to Nomarski. Here polarizer
and analyzer are at right angles to
chieved by adjusting the DIC slider.
Second : DIC with Sénarmont.
flexibility that will considerably
Instead of a DIC slider, a revolving
simplify your work.
analyzer with λ/4 plate is used to
attain contrast. The advantage of
the second method – considerably
increased accessibility – is particu-
Electrofusion of 2-cell stage
embryo, phase contrast,
K. Vintersten, S. Gray,
EMBL Heidelberg
larly evident in the case of larger,
lateral setups with cameras and
manipulators. Moreover, once the
small DIC sliders have been cen-
Normal embryo and
mutant, darkfield,
R. Adams,
EMBL Heidelberg
6
tered, they no longer have to be
adjusted.
Sénarmont analyzer
and DIC slider
7
Axiovert 200 –
The New Fluorescence
Innovations Well
Worth Looking at
Axiovert 200 offers yet more performance, intensity and homo-
Signaling Quality:
Greater Versatility:
The Light Trap
The New HBO Illuminator
with AttoArc 2
geneity when it comes to fluores-
Much more contrast with much
cence. It offers innovative ideas
and intelligent details which, taken
together, provide even higher
quality imaging as well as easier
and faster handling.
less stray light.
With the new AttoArc 2 you have
In other words: the best signal-to-
continuous brightness control of
noise ratio you’ll ever find. The
the HBO illuminator – directly from
patented Zeiss Light Trap makes
your PC via microscope software.
this possible by minimizing inter-
And there is yet another practical
fering stray light – and in the
innovation: The burner can be
process providing Axiovert 200
quickly and simply aligned with
with unprecedented brilliance in
the adjusting aid and lateral
fluorescence images.
adjusting screws of the new HBO
illuminator.
Just Push & Click :
Fluorescence to the
Power of Five
The new 5x reflector turret with
Push&Click filter sets
Five filter sets with full 23 mm field
Human endothelial cells,
4fold fluorescent
labeling,
J. Zbären,
Inselspital Bern,
Switzerland
of view – the Axiovert 200 reflector turret offers you more flexibility
and easier handling. The filter sets
can be switched within seconds –
Easily adjustable:
HBO illuminator with
lateral adjusting aid
(top)
Light trap:
less stray light,
more contrast
(bottom)
AttoArc 2
regulates intensity of
HBO illuminator
just push&click.
And if you want even more: A
motorized 8 x excitation filter is
available for Axiovert 200 M.
Soft Click :
Giving Smoothness
a New Twist
With Axiovert, rotating the reflector turret by one or more positions
is so exceptionally smooth that it
has earned a name of its own:
Axiovert 200 Soft Click. As a
result, you have vibration-free
micromanipulation and no capillary breakage.
8
9
Axiovert 200 –
The New Fluorescence
Innovations Well
Worth Looking at
Axiovert 200 offers yet more performance, intensity and homo-
Signaling Quality:
Greater Versatility:
The Light Trap
The New HBO Illuminator
with AttoArc 2
geneity when it comes to fluores-
Much more contrast with much
cence. It offers innovative ideas
and intelligent details which, taken
together, provide even higher
quality imaging as well as easier
and faster handling.
less stray light.
With the new AttoArc 2 you have
In other words: the best signal-to-
continuous brightness control of
noise ratio you’ll ever find. The
the HBO illuminator – directly from
patented Zeiss Light Trap makes
your PC via microscope software.
this possible by minimizing inter-
And there is yet another practical
fering stray light – and in the
innovation: The burner can be
process providing Axiovert 200
quickly and simply aligned with
with unprecedented brilliance in
the adjusting aid and lateral
fluorescence images.
adjusting screws of the new HBO
illuminator.
Just Push & Click :
Fluorescence to the
Power of Five
The new 5x reflector turret with
Push&Click filter sets
Five filter sets with full 23 mm field
Human endothelial cells,
4fold fluorescent
labeling,
J. Zbären,
Inselspital Bern,
Switzerland
of view – the Axiovert 200 reflector turret offers you more flexibility
and easier handling. The filter sets
can be switched within seconds –
Easily adjustable:
HBO illuminator with
lateral adjusting aid
(top)
Light trap:
less stray light,
more contrast
(bottom)
AttoArc 2
regulates intensity of
HBO illuminator
just push&click.
And if you want even more: A
motorized 8 x excitation filter is
available for Axiovert 200 M.
Soft Click :
Giving Smoothness
a New Twist
With Axiovert, rotating the reflector turret by one or more positions
is so exceptionally smooth that it
has earned a name of its own:
Axiovert 200 Soft Click. As a
result, you have vibration-free
micromanipulation and no capillary breakage.
8
9
Axiovert 200 –
The New Fluorescence
Highlights
in Easy Handling
The superb fluorescence of Axiovert 200
provides you with sharp contrast and un-
you will quickly appreciate in your daily
Speed with Safety:
Freedom:
The Shutter
The Reflector Turret
precedented image quality. It also provides
you with numerous practical features that
Flexibility Means
The standard electrical shutter in
Duodenum (rat),
J. Zbären,
Inselspital Bern
work.
Axiovert 200 M allows you to
the FL beampath guarantees that
change filter sets at the press of a
fluorescent dyes and samples will
button or by rotating them manu-
never fade. At the press of a but-
ally. It just depends on whether
ton on the front of the stand, the
you decide in favor of a manual or
shutter opens and closes the
motorized reflector turret. And
beampath quickly and safely.
should five reflector modules not
be enough, you can easily switch
Well Managed :
the manual reflector turret – either
The Light Manager
from the right or from the left,
The epi-fluorescence
beam path is optimized
for highest transmission
over a wide spectral range
– from UV to IR
whatever works best for your
Press a button and the halogen
experiment. A flexibility that is syn-
illuminator is easily switched on or
onymous with freedom, especially
off. With a special soft-start func-
in the case of large setups with
tion to protect the lamp – and your
micromanipulators. Proof yet again
eyes! Through simple storing, the
of the meticulous attention that
Light Manager makes it possible to
Axiovert 200 pays to those vital
use transmitted light and incident
details.
light either separately or simultaneously. Even the manual version
Endothelial cells,
J. Zbären,
Inselspital, Bern,
Switzerland
Practical and Pragmatic:
of Axiovert 200 offers this useful
The Sliders and
feature. The Light Manager in
Diaphragms
Axiovert 200 M offers you much
more: for example, storing of the
쑯 Aperture and luminous
field diaphragm slider,
filter slider
쑯 Ergonomic operation
of electric shutter
Optimal and proper Köhler illumi-
illuminator intensity depending on
nation, even in fluorescence. This
the Optovar used. Or it offers you
is achieved through centerable
contrasting methods with a motor-
aperture
ized condenser – depending on
and
luminous
field
diaphragms, which are available as
the objective used.
sliders and can easily be attached
to the stand from the side. A further advantage: the accessibility of
these diaphragm planes. Ideal
when you want to use slit diaphragms or pinhole diaphragms in
special applications.
10
11
Axiovert 200 –
The New Fluorescence
Highlights
in Easy Handling
The superb fluorescence of Axiovert 200
provides you with sharp contrast and un-
you will quickly appreciate in your daily
Speed with Safety:
Freedom:
The Shutter
The Reflector Turret
precedented image quality. It also provides
you with numerous practical features that
Flexibility Means
The standard electrical shutter in
Duodenum (rat),
J. Zbären,
Inselspital Bern
work.
Axiovert 200 M allows you to
the FL beampath guarantees that
change filter sets at the press of a
fluorescent dyes and samples will
button or by rotating them manu-
never fade. At the press of a but-
ally. It just depends on whether
ton on the front of the stand, the
you decide in favor of a manual or
shutter opens and closes the
motorized reflector turret. And
beampath quickly and safely.
should five reflector modules not
be enough, you can easily switch
Well Managed :
the manual reflector turret – either
The Light Manager
from the right or from the left,
The epi-fluorescence
beam path is optimized
for highest transmission
over a wide spectral range
– from UV to IR
whatever works best for your
Press a button and the halogen
experiment. A flexibility that is syn-
illuminator is easily switched on or
onymous with freedom, especially
off. With a special soft-start func-
in the case of large setups with
tion to protect the lamp – and your
micromanipulators. Proof yet again
eyes! Through simple storing, the
of the meticulous attention that
Light Manager makes it possible to
Axiovert 200 pays to those vital
use transmitted light and incident
details.
light either separately or simultaneously. Even the manual version
Endothelial cells,
J. Zbären,
Inselspital, Bern,
Switzerland
Practical and Pragmatic:
of Axiovert 200 offers this useful
The Sliders and
feature. The Light Manager in
Diaphragms
Axiovert 200 M offers you much
more: for example, storing of the
쑯 Aperture and luminous
field diaphragm slider,
filter slider
쑯 Ergonomic operation
of electric shutter
Optimal and proper Köhler illumi-
illuminator intensity depending on
nation, even in fluorescence. This
the Optovar used. Or it offers you
is achieved through centerable
contrasting methods with a motor-
aperture
ized condenser – depending on
and
luminous
field
diaphragms, which are available as
the objective used.
sliders and can easily be attached
to the stand from the side. A further advantage: the accessibility of
these diaphragm planes. Ideal
when you want to use slit diaphragms or pinhole diaphragms in
special applications.
10
11
Axiovert 200 –
The Interfaces
Ready for Your Goals
Almost unlimited possibilities –
both for documentation and for
manipulation. For the documentation of your work alone, Axiovert
200 provides you with six camera
Diverse :
The Optical Interfaces
Sideport left: With two or even three
positions for varying ratios.
interfaces – including dual video
Sideport right: With three positions
adapter. All controls are conve-
for varying ratios. The same applies to
niently arranged close to the front
the additional sideport left.
of the stand. This means: Switching
between single ports and varying
ratios is easy. And you decide which
Frontport. The frontport offers you
two adapters to connect a digital and
a video camera or an SLR camera.
step is the right one for your appli-
Possibilities for
mechanical adaptation
Everything is Possible :
Interfaces for the Manipulators
Baseport. The ideal interface for
cation.
extensive experimental setups, espe-
With its many mechanical inter-
Whether on the sides, on the illu-
cially for the simultaneous use of
faces, Axiovert 200 provides you
mination carrier or near the sur-
cameras and manipulators. And ideal,
with a wide range of adaptation
face of the table, whether conven-
if transmission should be as high as
possibilities – regardless of what
tional manipulation with capillaries
possible.
manipulator you are using.
and pipettes or with lasers of vary-
Dual Video Adapter. The adapter
ing wavelengths – Axiovert 200 is
for simultaneous imaging with two
ready to help you reach your goals.
cameras. Integrated Push&Click filter
sets for individual beam splitting.
The Dual Video Adapter:
For simultaneous documentation
with two cameras
Precisely: one TV port is adjustable in
x, y and z direction.
Control elements
for TV ports:
manual stand
쑲
motorized stand 쑲
12
2-cell mouse embryo,
DIC, D.L. Keefe,
MBL, Woods Hole,
USA
Injection in
Blastocytes, DIC,
K. Vintersten,
S. Gray, EMBL
Heidelberg,
Germany
13
Axiovert 200 –
The Interfaces
Ready for Your Goals
Almost unlimited possibilities –
both for documentation and for
manipulation. For the documentation of your work alone, Axiovert
200 provides you with six camera
Diverse :
The Optical Interfaces
Sideport left: With two or even three
positions for varying ratios.
interfaces – including dual video
Sideport right: With three positions
adapter. All controls are conve-
for varying ratios. The same applies to
niently arranged close to the front
the additional sideport left.
of the stand. This means: Switching
between single ports and varying
ratios is easy. And you decide which
Frontport. The frontport offers you
two adapters to connect a digital and
a video camera or an SLR camera.
step is the right one for your appli-
Possibilities for
mechanical adaptation
Everything is Possible :
Interfaces for the Manipulators
Baseport. The ideal interface for
cation.
extensive experimental setups, espe-
With its many mechanical inter-
Whether on the sides, on the illu-
cially for the simultaneous use of
faces, Axiovert 200 provides you
mination carrier or near the sur-
cameras and manipulators. And ideal,
with a wide range of adaptation
face of the table, whether conven-
if transmission should be as high as
possibilities – regardless of what
tional manipulation with capillaries
possible.
manipulator you are using.
and pipettes or with lasers of vary-
Dual Video Adapter. The adapter
ing wavelengths – Axiovert 200 is
for simultaneous imaging with two
ready to help you reach your goals.
cameras. Integrated Push&Click filter
sets for individual beam splitting.
The Dual Video Adapter:
For simultaneous documentation
with two cameras
Precisely: one TV port is adjustable in
x, y and z direction.
Control elements
for TV ports:
manual stand
쑲
motorized stand 쑲
12
2-cell mouse embryo,
DIC, D.L. Keefe,
MBL, Woods Hole,
USA
Injection in
Blastocytes, DIC,
K. Vintersten,
S. Gray, EMBL
Heidelberg,
Germany
13
The Ergonomics
Benefits Within
Your Grasp
The
success
of
your
work
depends on easy and fatigue-free
operation of a microscope. And
through its new ergonomics,
Axiovert 200 helps ensure your
success. With benefits that are
literally within your grasp: the
convenient, easy-to-use arrangement of the controls and the
smooth and rapid switching
functions. And, of course, with
careful attention paid to important details.
All in all, Axiovert 200 offers you
a wealth of sophisticated ergonomic benefits you will appreciate
after a long day’s work.
Help in Sight :
Powerful Protection :
The Optical Components
Aqua Stop
Now true help with your work is
The idea is simple, the perfor-
finally in sight: The analyzer slider
mance powerful: Aqua Stop, the
with Sénarmont, easily accessible
latest Zeiss innovation. It protects
and operable from both sides,
objectives and stands from any
guarantees rapid contrast adjust-
liquids that have been spilled.
ment. The Light Manager, which
Through a triple protection system.
stores lamp intensities, makes it
First, through the silicon sealing
even easier for you to carry out
directly on the objective. Second,
your work.
through a silicon coating that covers the entire objective turret. And
From Ergotube to
third, through a container placed
Object Guide : The
under the objective turret. Two
Mechanical Components
tubes leading to it channel the
liquid away from the microscope.
Individually
adjusted
to
every
And best of all: Even with Aqua
height – the Ergotube is continu-
Stop the objective turret remains
ously adjustable. Maintaining all the
completely rotary.
Everything Easily
while a convenient 25° viewing
Under Control:
angle. There are other impressive
The Electric Components
examples of how Axiovert 200
meets individual needs. It doesn’t
All controls directly near the focus-
matter, for example, if you are
ing drive – whether the toggle for
right-handed or left-handed: The
lamp intensity, electric shutter for
object guide can be located either
fluorescence light path, buttons
on the right or the left of the
for the halogen lamp (on/off), tube
stage. And the focus stop guaran-
lens or sideport turret and base-
tees that the objective turret auto-
port slider. So that you have every-
matically stops before the objec-
thing easily under control.
tive touches the sample.
Everything at a Glance:
The LCD Display
Just one quick look is enough: the
LCD display shows you the current
status of the microscope adjustments – whether it’s magnification, lamp intensity, shutter posi1
tion or the z measuring function to
determine specimen thickness. At
2
3
4
a glance everything you need to
carry out your work rapidly and
precisely.
14
1) height adjustable Ergotube
2) Just push a button – Switching between
fluorescence and brightfield
3) One touch – focussing and turning
the reflector turret
4) One turn – easy adjusting of
DIC contrast with the Sénarmont analyzer
15
The Ergonomics
Benefits Within
Your Grasp
The
success
of
your
work
depends on easy and fatigue-free
operation of a microscope. And
through its new ergonomics,
Axiovert 200 helps ensure your
success. With benefits that are
literally within your grasp: the
convenient, easy-to-use arrangement of the controls and the
smooth and rapid switching
functions. And, of course, with
careful attention paid to important details.
All in all, Axiovert 200 offers you
a wealth of sophisticated ergonomic benefits you will appreciate
after a long day’s work.
Help in Sight :
Powerful Protection :
The Optical Components
Aqua Stop
Now true help with your work is
The idea is simple, the perfor-
finally in sight: The analyzer slider
mance powerful: Aqua Stop, the
with Sénarmont, easily accessible
latest Zeiss innovation. It protects
and operable from both sides,
objectives and stands from any
guarantees rapid contrast adjust-
liquids that have been spilled.
ment. The Light Manager, which
Through a triple protection system.
stores lamp intensities, makes it
First, through the silicon sealing
even easier for you to carry out
directly on the objective. Second,
your work.
through a silicon coating that covers the entire objective turret. And
From Ergotube to
third, through a container placed
Object Guide : The
under the objective turret. Two
Mechanical Components
tubes leading to it channel the
liquid away from the microscope.
Individually
adjusted
to
every
And best of all: Even with Aqua
height – the Ergotube is continu-
Stop the objective turret remains
ously adjustable. Maintaining all the
completely rotary.
Everything Easily
while a convenient 25° viewing
Under Control:
angle. There are other impressive
The Electric Components
examples of how Axiovert 200
meets individual needs. It doesn’t
All controls directly near the focus-
matter, for example, if you are
ing drive – whether the toggle for
right-handed or left-handed: The
lamp intensity, electric shutter for
object guide can be located either
fluorescence light path, buttons
on the right or the left of the
for the halogen lamp (on/off), tube
stage. And the focus stop guaran-
lens or sideport turret and base-
tees that the objective turret auto-
port slider. So that you have every-
matically stops before the objec-
thing easily under control.
tive touches the sample.
Everything at a Glance:
The LCD Display
Just one quick look is enough: the
LCD display shows you the current
status of the microscope adjustments – whether it’s magnification, lamp intensity, shutter posi1
tion or the z measuring function to
determine specimen thickness. At
2
3
4
a glance everything you need to
carry out your work rapidly and
precisely.
14
1) height adjustable Ergotube
2) Just push a button – Switching between
fluorescence and brightfield
3) One touch – focussing and turning
the reflector turret
4) One turn – easy adjusting of
DIC contrast with the Sénarmont analyzer
15
Axiovert 200 M
Motorized Top Performance
The Best in Live Cell
In its manual version Axiovert 200
Imaging :
already makes your work a lot eas-
Motorization times 8
ier. But if you want to exploit its
full potential, then you need
Simplifies and speeds up complex
Axiovert 200 M, the fully motor-
operations: With up to eight coded
ized version. Due to its modular
and fully motorized components,
construction it is upgradable at
Axiovert 200 M provides the best
any time according to your needs
conditions for the automation of
and budget with a motorized con-
live cell imaging applications:
denser or a motorized reflector
turret. All the motorized functions
• Automatic adjustment of objec-
are controlled either by buttons on
tive and reflector turret
the stand itself – or via the RS232
• Automatic setting of previously
interface with PC and corresponding
software,
for
selected diaphragms and bright-
example,
ness levels via Light Manager
AxioVisionControl.
• Choice of five documentation
ports via the Electronic Port
System (EPS)
• Secondary 1.6x and 2.5x magni-
Axiovert 200 M from the back:
Interfaces for external components
fication through motorized
Optovar system
• Pushbutton controlled fluorescence and halogen lamp shutters
• Z focus with the high-precision
Harmonic Drive DC motor for
scanning Z specimens with minimum step size of 25 nm, ideal for
deconvolution, 6-D imaging and
confocal microscopy with LSM 510
and LSM 5 Pascal
AxioVisionControl:
Perfect software control
from Carl Zeiss
16
17
Axiovert 200 M
Motorized Top Performance
The Best in Live Cell
In its manual version Axiovert 200
Imaging :
already makes your work a lot eas-
Motorization times 8
ier. But if you want to exploit its
full potential, then you need
Simplifies and speeds up complex
Axiovert 200 M, the fully motor-
operations: With up to eight coded
ized version. Due to its modular
and fully motorized components,
construction it is upgradable at
Axiovert 200 M provides the best
any time according to your needs
conditions for the automation of
and budget with a motorized con-
live cell imaging applications:
denser or a motorized reflector
turret. All the motorized functions
• Automatic adjustment of objec-
are controlled either by buttons on
tive and reflector turret
the stand itself – or via the RS232
• Automatic setting of previously
interface with PC and corresponding
software,
for
selected diaphragms and bright-
example,
ness levels via Light Manager
AxioVisionControl.
• Choice of five documentation
ports via the Electronic Port
System (EPS)
• Secondary 1.6x and 2.5x magni-
Axiovert 200 M from the back:
Interfaces for external components
fication through motorized
Optovar system
• Pushbutton controlled fluorescence and halogen lamp shutters
• Z focus with the high-precision
Harmonic Drive DC motor for
scanning Z specimens with minimum step size of 25 nm, ideal for
deconvolution, 6-D imaging and
confocal microscopy with LSM 510
and LSM 5 Pascal
AxioVisionControl:
Perfect software control
from Carl Zeiss
16
17
The Stages
The Basis for Your
Research
The right stage for every application, the right mounting frame
for every specimen – Carl Zeiss
has a broad spectrum of acces-
The Specimen Stage
Whether your specimen needs
With attachable object guide (right-
heating or cooling – Carl Zeiss has
or left-hand) the ideal solution for
the facilities to optimize the envi-
many applications.
ronment for any experimental
setup: Special heating and temper-
sories precisely matched to the
microscope and satisfying your
most critical requirements.
Temperature Control and
Incubation
The Right Climate
The Mechanical Stage
ature controlled stages. Or, for
Latest version of a successful con-
upgrading a plain or mechanical
cept: with plane surface, plus the
stage: heatable and temperature
extra-long travels and utmost
controlled mounting frames are
precision required for multi-well
available.
plates.
The Carl Zeiss heating stage generates heat through transistor heat
The Gliding Stage
loss – without interfering electric
The top plate with your specimen
fields. This is ideal for electrophys-
can be sensitively shifted in any
iology. In addition, the stage has
direction as well as rotated. The
ducts which direct a heated air
optimum solution for specimen
blast under the specimen, right
positioning prior to micromanipu-
where it is needed.
lation and for chromosome micro-
An alternative way of temperature
dissection.
control is heat flow past the specimen within an incubator. This way,
The Motorized
a temperature gradient cannot
Scanning Stage
build up in the first place. The
PC- or joystick-controlled, this
heating effect is supplemented by
stage allows highly precise posi-
objective heaters which attach to
tioning of specimen details. Soft-
immersion objectives. The heat is
ware modules such as Mark&Find
transmitted through the immer-
in AxioVision handle the saving and
sion oil right up to the specimen.
retrieving of positions.
In addition to the right tempera-
탊 Incubator XL: This large incubator for all
stages and various manipulators encloses most
of the microscope. A generous number of doors
provides easy access to the interior.
탋 Incubator M: Medium-size incubator for the
plain and heating stages with attached object guide.
Easy micromanipulation through an opening on
the left side.
ture, constant pH is essential for
With any stage you can select from
optimum cell observation. This is
a wide variety of mounting frames
ensured by incubation with CO2.
for specimen slides, Petri dishes
Choose from the Carl Zeiss incuba-
and vessels of various shapes.
tors shown on the right.
Universal mounting frames are
specially recommended for their
versatility. For outstanding precision, choose:
– M for the plain stage,
– K for the mechanical stage,
– MX or KX for multi-well plates.
18
19
The Stages
The Basis for Your
Research
The right stage for every application, the right mounting frame
for every specimen – Carl Zeiss
has a broad spectrum of acces-
The Specimen Stage
Whether your specimen needs
With attachable object guide (right-
heating or cooling – Carl Zeiss has
or left-hand) the ideal solution for
the facilities to optimize the envi-
many applications.
ronment for any experimental
setup: Special heating and temper-
sories precisely matched to the
microscope and satisfying your
most critical requirements.
Temperature Control and
Incubation
The Right Climate
The Mechanical Stage
ature controlled stages. Or, for
Latest version of a successful con-
upgrading a plain or mechanical
cept: with plane surface, plus the
stage: heatable and temperature
extra-long travels and utmost
controlled mounting frames are
precision required for multi-well
available.
plates.
The Carl Zeiss heating stage generates heat through transistor heat
The Gliding Stage
loss – without interfering electric
The top plate with your specimen
fields. This is ideal for electrophys-
can be sensitively shifted in any
iology. In addition, the stage has
direction as well as rotated. The
ducts which direct a heated air
optimum solution for specimen
blast under the specimen, right
positioning prior to micromanipu-
where it is needed.
lation and for chromosome micro-
An alternative way of temperature
dissection.
control is heat flow past the specimen within an incubator. This way,
The Motorized
a temperature gradient cannot
Scanning Stage
build up in the first place. The
PC- or joystick-controlled, this
heating effect is supplemented by
stage allows highly precise posi-
objective heaters which attach to
tioning of specimen details. Soft-
immersion objectives. The heat is
ware modules such as Mark&Find
transmitted through the immer-
in AxioVision handle the saving and
sion oil right up to the specimen.
retrieving of positions.
In addition to the right tempera-
탊 Incubator XL: This large incubator for all
stages and various manipulators encloses most
of the microscope. A generous number of doors
provides easy access to the interior.
탋 Incubator M: Medium-size incubator for the
plain and heating stages with attached object guide.
Easy micromanipulation through an opening on
the left side.
ture, constant pH is essential for
With any stage you can select from
optimum cell observation. This is
a wide variety of mounting frames
ensured by incubation with CO2.
for specimen slides, Petri dishes
Choose from the Carl Zeiss incuba-
and vessels of various shapes.
tors shown on the right.
Universal mounting frames are
specially recommended for their
versatility. For outstanding precision, choose:
– M for the plain stage,
– K for the mechanical stage,
– MX or KX for multi-well plates.
18
19
Carl Zeiss – Digital Technology
Intelligence in Light Microscopy
Division of a CHO cell.
Bottom left: Original data
Upper right: Result after deconvolution
AxioCam :
We all know that microscopy
The High-end Camera for
today could not exist without
a High-end Microscope
the support of computers – and
that many applications would
Perfect for every single one of your
not even be feasible. Carl Zeiss
applications – the highly sensitive
has developed a tailor-made
digital camera AxioCam enables
package of solutions comprising
you to exploit the full resolution
software with flexible modular
capacity of Axiovert 200. With
components and a digital cam-
ultrahigh resolution up to 3900 x
era. In combination with the
3090 pixels in real color and high
inverted high-end Axiovert 200
dynamics for high-quality fluores-
microscope, this package provides you with the best basis for
successful work in microscopy
today.
Optimal Fluorescence:
the cooled camera generates nee-
Deconvolution
dle-sharp, color-fast images in all
from Carl Zeiss
image formats. Even extremely
Everything Under
Control :
Simple and
clear operation
of AxioVision
software
AxioVision Control
fine structures are reproduced
You are only too well familiar with
without color moiré. AxioCam is
this problem in fluorescence micros-
pleasantly easy to use. It works
copy: stray light from above and
without an external control box
below the focal plane. Your image
and is absolutely vibration-free.
is over-exposed – to the extent
With approximately 20 images per
that in the worst of cases you no
second on your monitor, the live
longer recognize any structures.
mode is very rapid. You can focus
The powerful software solution
precisely (in color!), look for the
from Carl Zeiss can calculate the
right place in the specimen and
interfering stray light right back to
All the motorized functions of your
check exposure time. AxioCam, a
the point of origin. As a conse-
microscope perfectly controlled:
real multitalent and an outstand-
quence, the distorted structure
Through
ing member of the Axiovert 200
“straightens out”. With the help of
its
exceptional
user-
friendliness, AxioVisionControl is
team. Available as AxioCam color,
Z-stack modules and 3-D deconvo-
ideal for multi-user operations.
Digital Top Performance :
the superbly flexible color camera
lution, AxioVision takes care of
Storing and recalling parameters
AxioVision
for transmitted light and fluores-
everything. First, the control and
cence applications with high re-
regulation of microscope and cam-
for reproducible re-use, selection
20
cence (14 bits per color channel),
of contrasting methods, reflector
Convenient controls and precise
magnification factors or scale dis-
trols and optimal adaptation to
solution. Or as AxioCam black &
era in capturing the Z-stacks.
cubes, objectives or complete
image processing – that’s what
play when changing objectives
individual applications. Moreover,
white with extra high sensitivity
Second, calculating the degree of
adjustments – these are only a
AxioVision, the digital imaging sys-
right up to reports. Since its main
it is possible to add on other help-
for such fluorescence applications
distortion (PSF – Point Spread Func-
few
as FISH or deconvolution.
that
tem from Carl Zeiss, offers you.
window is divided into two areas,
ful features. Additional modules
AxioVisionControl takes over for
From image acquisition, process-
one for working and the other for
provide you with even more possi-
method. The result is significant
you. Fast and easy at the press of a
ing, annotation and archiving, to
documentation, AxioVision pro-
bilities to process and analyze
increases in contrast and sharper,
button.
automatic storage and retention of
vides both easy-to-operate con-
images.
clearer images. Fast and easy.
of
the
operations
tion). Third, the deconvolution
21
Carl Zeiss – Digital Technology
Intelligence in Light Microscopy
Division of a CHO cell.
Bottom left: Original data
Upper right: Result after deconvolution
AxioCam :
We all know that microscopy
The High-end Camera for
today could not exist without
a High-end Microscope
the support of computers – and
that many applications would
Perfect for every single one of your
not even be feasible. Carl Zeiss
applications – the highly sensitive
has developed a tailor-made
digital camera AxioCam enables
package of solutions comprising
you to exploit the full resolution
software with flexible modular
capacity of Axiovert 200. With
components and a digital cam-
ultrahigh resolution up to 3900 x
era. In combination with the
3090 pixels in real color and high
inverted high-end Axiovert 200
dynamics for high-quality fluores-
microscope, this package provides you with the best basis for
successful work in microscopy
today.
Optimal Fluorescence:
the cooled camera generates nee-
Deconvolution
dle-sharp, color-fast images in all
from Carl Zeiss
image formats. Even extremely
Everything Under
Control :
Simple and
clear operation
of AxioVision
software
AxioVision Control
fine structures are reproduced
You are only too well familiar with
without color moiré. AxioCam is
this problem in fluorescence micros-
pleasantly easy to use. It works
copy: stray light from above and
without an external control box
below the focal plane. Your image
and is absolutely vibration-free.
is over-exposed – to the extent
With approximately 20 images per
that in the worst of cases you no
second on your monitor, the live
longer recognize any structures.
mode is very rapid. You can focus
The powerful software solution
precisely (in color!), look for the
from Carl Zeiss can calculate the
right place in the specimen and
interfering stray light right back to
All the motorized functions of your
check exposure time. AxioCam, a
the point of origin. As a conse-
microscope perfectly controlled:
real multitalent and an outstand-
quence, the distorted structure
Through
ing member of the Axiovert 200
“straightens out”. With the help of
its
exceptional
user-
friendliness, AxioVisionControl is
team. Available as AxioCam color,
Z-stack modules and 3-D deconvo-
ideal for multi-user operations.
Digital Top Performance :
the superbly flexible color camera
lution, AxioVision takes care of
Storing and recalling parameters
AxioVision
for transmitted light and fluores-
everything. First, the control and
cence applications with high re-
regulation of microscope and cam-
for reproducible re-use, selection
20
cence (14 bits per color channel),
of contrasting methods, reflector
Convenient controls and precise
magnification factors or scale dis-
trols and optimal adaptation to
solution. Or as AxioCam black &
era in capturing the Z-stacks.
cubes, objectives or complete
image processing – that’s what
play when changing objectives
individual applications. Moreover,
white with extra high sensitivity
Second, calculating the degree of
adjustments – these are only a
AxioVision, the digital imaging sys-
right up to reports. Since its main
it is possible to add on other help-
for such fluorescence applications
distortion (PSF – Point Spread Func-
few
as FISH or deconvolution.
that
tem from Carl Zeiss, offers you.
window is divided into two areas,
ful features. Additional modules
AxioVisionControl takes over for
From image acquisition, process-
one for working and the other for
provide you with even more possi-
method. The result is significant
you. Fast and easy at the press of a
ing, annotation and archiving, to
documentation, AxioVision pro-
bilities to process and analyze
increases in contrast and sharper,
button.
automatic storage and retention of
vides both easy-to-operate con-
images.
clearer images. Fast and easy.
of
the
operations
tion). Third, the deconvolution
21
Carl Zeiss – Live Cell Imaging
Seeing Life Through New Eyes
1
2
Central control of the most
important adjustments for
multidimensional imaging
Bordering on In Vivo reality –
experiments with living cells and
tissues are confronting researchers with real challenges. Cell
Observer is the first complete
solution for the observation and
digital documentation of living
processes. Ready for use, and
rigorously tailored to the exacting requirements made by the
All in One:
alter all parameters even while
New Dimensions:
The Functions
recording through “Pause” and
The Performance
“Continue” functions. Or measurDocumenting dynamic processes
ing the distance from A to B, cal-
with time-lapse, multi-channel, z-
culating the angle between A, B
stack and Mark&Find in freely
and C, statistics and much, much
selectable combinations – Cell
more. In a nutshell: All the fea-
Observer offers you everything in
tures that you expect and need in
one system. Including such practi-
modern live cell imaging.
3
When you go beyond the second
4
3
and third dimensions, you see with
Hela cells in phase contrast and
GFP fluorescence
(Golgi markings).
Dissociation of Golgi apparatus
according to Brefeldin A Zugabe.
Dr. Pepperkok,
EMBL Heidelberg, Germany
striking clarity the new dimensions
in performance that Cell Observer
offers you. The information found
in the fourth wavelength dimen-
cal features as the possibility to
sion can be stored in 8 different
channels of the image and freely
varying applications in live cell
combined at any time. The fifth
imaging. And there’s another big
time
plus: Since Cell Observer is easy
sion various positions are automat-
its many top-of-the-line fea-
ically brought into focus on the
tures. The best basis for out-
culture plate. Whether you’re
standing success in cell and mol-
Teamwork:
The Components
Cell Observer is the first powerful,
the
period of time. In the sixth dimen-
even beginners can benefit from
Focus on Perfect
describes
recording of cells during a defined
to operate and user-friendly,
ecular biology.
dimension
working in 2-D or in high-end 6-D
Axiovert 200 with scanning
stage and Incubator S
which is attached to the heating
insert P for mechanical and
scanning stages. Advantage:
small volume, significantly
reduced CO2 consumption,
imaging of diverse cell culture
chambers, ideal for long-term
experiments
imaging, with 6 images or 600:
Cell Observer enables you to experience totally new dimensions in
live cell imaging.
Cell Observer : The Applications
complete and homogeneous live
cell imaging system. A combination of perfectly matched components, designed to meet all demands. The system consists of: a
Zeiss microscope, the Zeiss digital
camera AxioCam, the Zeiss software system AxioVision as well as
Zeiss accessories such as x, ystages, filter wheels, shutters,
incubators and culture chambers.
22
Transmitted Light
• Observation over time
• Observation over time with interruptions and manipulation
• Observation over time with motorized x,y-stage
• Observation over time of dynamic processes
• Observation over time Z-stack
Fluorescence
• Observation over time in up to 8 channels (fluorescence or transmitted light)
• Observation over time in up to 8 channels with interruptions and manipulation
• Observation over time in up to 8 channels with motorized x-, y-stage
• Observation over time in up to 8 channels with Z-stack
23
Carl Zeiss – Live Cell Imaging
Seeing Life Through New Eyes
1
2
Central control of the most
important adjustments for
multidimensional imaging
Bordering on In Vivo reality –
experiments with living cells and
tissues are confronting researchers with real challenges. Cell
Observer is the first complete
solution for the observation and
digital documentation of living
processes. Ready for use, and
rigorously tailored to the exacting requirements made by the
All in One:
alter all parameters even while
New Dimensions:
The Functions
recording through “Pause” and
The Performance
“Continue” functions. Or measurDocumenting dynamic processes
ing the distance from A to B, cal-
with time-lapse, multi-channel, z-
culating the angle between A, B
stack and Mark&Find in freely
and C, statistics and much, much
selectable combinations – Cell
more. In a nutshell: All the fea-
Observer offers you everything in
tures that you expect and need in
one system. Including such practi-
modern live cell imaging.
3
When you go beyond the second
4
3
and third dimensions, you see with
Hela cells in phase contrast and
GFP fluorescence
(Golgi markings).
Dissociation of Golgi apparatus
according to Brefeldin A Zugabe.
Dr. Pepperkok,
EMBL Heidelberg, Germany
striking clarity the new dimensions
in performance that Cell Observer
offers you. The information found
in the fourth wavelength dimen-
cal features as the possibility to
sion can be stored in 8 different
channels of the image and freely
varying applications in live cell
combined at any time. The fifth
imaging. And there’s another big
time
plus: Since Cell Observer is easy
sion various positions are automat-
its many top-of-the-line fea-
ically brought into focus on the
tures. The best basis for out-
culture plate. Whether you’re
standing success in cell and mol-
Teamwork:
The Components
Cell Observer is the first powerful,
the
period of time. In the sixth dimen-
even beginners can benefit from
Focus on Perfect
describes
recording of cells during a defined
to operate and user-friendly,
ecular biology.
dimension
working in 2-D or in high-end 6-D
Axiovert 200 with scanning
stage and Incubator S
which is attached to the heating
insert P for mechanical and
scanning stages. Advantage:
small volume, significantly
reduced CO2 consumption,
imaging of diverse cell culture
chambers, ideal for long-term
experiments
imaging, with 6 images or 600:
Cell Observer enables you to experience totally new dimensions in
live cell imaging.
Cell Observer : The Applications
complete and homogeneous live
cell imaging system. A combination of perfectly matched components, designed to meet all demands. The system consists of: a
Zeiss microscope, the Zeiss digital
camera AxioCam, the Zeiss software system AxioVision as well as
Zeiss accessories such as x, ystages, filter wheels, shutters,
incubators and culture chambers.
22
Transmitted Light
• Observation over time
• Observation over time with interruptions and manipulation
• Observation over time with motorized x,y-stage
• Observation over time of dynamic processes
• Observation over time Z-stack
Fluorescence
• Observation over time in up to 8 channels (fluorescence or transmitted light)
• Observation over time in up to 8 channels with interruptions and manipulation
• Observation over time in up to 8 channels with motorized x-, y-stage
• Observation over time in up to 8 channels with Z-stack
23
Axiovert 200 M –
Laser Scanning Microscopy
See More, Recognize More
2
To give you an edge in your
biomedical research, use the
Axiovert 200 top-of-the-line
research microscope as part of
your confocal system.
The optics and mechanics of the
Axiovert 200 have been designed to integrate effortlessly
embryos. Its multiphoton technol-
Intelligent Features :
ogy guarantees excellent imaging
The Intuitive Software
even with thicker samples. And its
high 3-D selectivity enables you to
The sophisticated and continually
conduct bleaching experiments
expanding LSM software can do
such as FRAP and uncaging locally
far more than record brilliant
in addition to flexible image acqui-
images. Measurement functions
sition.
are available to evaluate your data
while an entire series of different
into the Zeiss LSM 510 confocal
microscope and its “little brother”
the LSM 5 PASCAL. For outstanding resolution in all dimensions,
LSM 510 and LSM 5 PASCAL are
your ideal partners. Their powerful performance is still unequaled.
Efficient and Easy
3-D and 4-D visualization features
on the Sample :
provide you with totally new
1
Flexibility of Application
No other LSM system offers you so
many different scanning methods:
scan along a freehand drawn line,
complete 360° image rotation,
zoom and offset. In addition you
have standard and special modes
such as spline scan or Real Region
Growing with
of Interest (ROI) scan. Even com-
Your Requirements :
plex time series can easily be
The LSM Family
defined and captured – with special attention paid to careful han-
insights into the structure of your
The LSM 5 PASCAL is an the ideal
dling of your samples. All process-
samples. Numerous online and
entry-level confocal microscope – a
es can be recorded and stored as
offline evaluation features analyze
powerful but budget-priced sys-
digital movies. Thanks to the mul-
time series. The extremely user-
tem that sets new standards in its
titracking features these LSM sys-
friendly
class. The LSM 5 PASCAL is the
tems offer, you can acquire multi-
stores not only image data but also
microscope of choice when you
channel fluorescence images with-
all relevant parameters, including
need a wide spectrum of fluores-
out channel crosstalk. All of this
your notes. And because the sys-
cence applications, including DIC
underscores the philosophy behind
tem
contrast images. And its “big
Zeiss LSM technology: noise-free
reproducing an experiment under
brother”, the powerful Zeiss LSM
images with a minimum of effort.
identical conditions is only a
is
LSM
image
completely
database
3
4
1) Drosophila embryo,
optical sections (slices)
and projection of a
stack of 47 images.
Cell nuclei (propidium
iodide), chromatin
protein (FITC).
Molecular Interactions
2) Mouse intestine
section, double fluorescence, 3-D shadow
projection
for cells: with the LSM 510 you can
3) Bovine endothelial
cells, triple fluorescence,
nucleus (DAPI)
F-actin (BODIPY-FL)
mitochondria
(Mitotracker Red)
troscopy (FCS), ConfoCor 2 helps
Efficiently Analyzed :
ConfoCor 2
Universal for solutions, unbeatable
localize marked molecules. Using
fluorescence
correlation
spec-
you to understand the interactions
between these molecules. The
measuring volume is, smaller than
4) Leaf tissue with
an E. coli bacterium and fits in
vascular bundle,
double fluorescence and every cell. As a result, biochemical
differential interference processes can be examined in their
contrast (DIC)
natural environment. Of course,
you can also use ConfoCor 2 for
measurements in solutions.
motorized,
510, offers even more possibilities.
mouse click away. This makes the
With up to four fluorescence chan-
work significantly easier and saves
nels simultaneously, and a wide
more than just time.
choice of lasers to excite dyes in
the UV and VIS range. LSM 510
NLO is perfect for the long-term
observation of cells, tissues and
24
25
Axiovert 200 M –
Laser Scanning Microscopy
See More, Recognize More
2
To give you an edge in your
biomedical research, use the
Axiovert 200 top-of-the-line
research microscope as part of
your confocal system.
The optics and mechanics of the
Axiovert 200 have been designed to integrate effortlessly
embryos. Its multiphoton technol-
Intelligent Features :
ogy guarantees excellent imaging
The Intuitive Software
even with thicker samples. And its
high 3-D selectivity enables you to
The sophisticated and continually
conduct bleaching experiments
expanding LSM software can do
such as FRAP and uncaging locally
far more than record brilliant
in addition to flexible image acqui-
images. Measurement functions
sition.
are available to evaluate your data
while an entire series of different
into the Zeiss LSM 510 confocal
microscope and its “little brother”
the LSM 5 PASCAL. For outstanding resolution in all dimensions,
LSM 510 and LSM 5 PASCAL are
your ideal partners. Their powerful performance is still unequaled.
Efficient and Easy
3-D and 4-D visualization features
on the Sample :
provide you with totally new
1
Flexibility of Application
No other LSM system offers you so
many different scanning methods:
scan along a freehand drawn line,
complete 360° image rotation,
zoom and offset. In addition you
have standard and special modes
such as spline scan or Real Region
Growing with
of Interest (ROI) scan. Even com-
Your Requirements :
plex time series can easily be
The LSM Family
defined and captured – with special attention paid to careful han-
insights into the structure of your
The LSM 5 PASCAL is an the ideal
dling of your samples. All process-
samples. Numerous online and
entry-level confocal microscope – a
es can be recorded and stored as
offline evaluation features analyze
powerful but budget-priced sys-
digital movies. Thanks to the mul-
time series. The extremely user-
tem that sets new standards in its
titracking features these LSM sys-
friendly
class. The LSM 5 PASCAL is the
tems offer, you can acquire multi-
stores not only image data but also
microscope of choice when you
channel fluorescence images with-
all relevant parameters, including
need a wide spectrum of fluores-
out channel crosstalk. All of this
your notes. And because the sys-
cence applications, including DIC
underscores the philosophy behind
tem
contrast images. And its “big
Zeiss LSM technology: noise-free
reproducing an experiment under
brother”, the powerful Zeiss LSM
images with a minimum of effort.
identical conditions is only a
is
LSM
image
completely
database
3
4
1) Drosophila embryo,
optical sections (slices)
and projection of a
stack of 47 images.
Cell nuclei (propidium
iodide), chromatin
protein (FITC).
Molecular Interactions
2) Mouse intestine
section, double fluorescence, 3-D shadow
projection
for cells: with the LSM 510 you can
3) Bovine endothelial
cells, triple fluorescence,
nucleus (DAPI)
F-actin (BODIPY-FL)
mitochondria
(Mitotracker Red)
troscopy (FCS), ConfoCor 2 helps
Efficiently Analyzed :
ConfoCor 2
Universal for solutions, unbeatable
localize marked molecules. Using
fluorescence
correlation
spec-
you to understand the interactions
between these molecules. The
measuring volume is, smaller than
4) Leaf tissue with
an E. coli bacterium and fits in
vascular bundle,
double fluorescence and every cell. As a result, biochemical
differential interference processes can be examined in their
contrast (DIC)
natural environment. Of course,
you can also use ConfoCor 2 for
measurements in solutions.
motorized,
510, offers even more possibilities.
mouse click away. This makes the
With up to four fluorescence chan-
work significantly easier and saves
nels simultaneously, and a wide
more than just time.
choice of lasers to excite dyes in
the UV and VIS range. LSM 510
NLO is perfect for the long-term
observation of cells, tissues and
24
25
Axiovert 200 –
A Partnership for the Future
Ideally Suited for
That Extra Plus
the Perfect Handshake :
in Performance :
Zeiss Partners
Zeiss Service
Convincing from A – Z
the diaphragm planes
Complete
system solutions from a single
source
Whenever you need special soft-
The decision you make for a micro-
enable you to make great progress
ware for your applications, remem-
scope, one equipped with the
in your work. As a matter of fact,
Confocal
with LSM 510
the Light Trap
ber: Your partners are our part-
components you need, is as com-
you often can’t really appreciate
ners. Axiovert 200‘s open archi-
plex as the requirements it must
the full range of Zeiss service until
Contrastenhancing
tecture makes it easy for you to
meet. A skilled team of consul-
after you have made your pur-
Convenient
the Light Manager
effortlessly link software packages
tants will help you with making
chase – in practice, which is what
Customized
the modular system
AxioVisionControl software
from other suppliers. Which of
the right choice and with budget
counts. Our local consultants and
Digital
course makes Axiovert 200 the
planning. And all of our consul-
technicians and the Carl Zeiss cus-
Easy to use
the Push&Click filter cubes
the motorized features
ideal platform for a wide range of
tants possess impressive know-
tomer service all support you in
Efficient
special application driven software
how and experience as well as
your research with technical and
Ergonomic
the Ergotube
extensive knowledge of the entire
applications aid, whenever you
Flexible
the Dual Video Adapter
microscope market. Thus you ben-
need it. Fast and reliably. More-
Generous
efit from much more than our skill
over, Carl Zeiss training courses
the working distances of the
LD Condensers
in developing microscopes. You
and workshops provide you with
will be able to draw upon the
added insights into practical areas
enormous wealth of experience
of microscopy and imaging tech-
that Zeiss has accumulated in
niques. In fact, when you add to-
decades of practice in research and
gether all the services and support
routine. You will profit from con-
that you get with Axiovert 200,
crete assistance in your microscopy
you’ll see that it is more than a
methods – and above all from
high-power microscope:
innovative methods which will
Axiovert 200 is your ticket to
in many areas of research.
a powerhouse of knowledge in
microscopy that has been built up
over the past 150 years.
26
Accessible
Highly resolved DIC (even with Sénarmont)
Ideal
the documentation ports
Individual
the function buttons
Informative
the LCD display
N-dimensional
the modular Cell Observer
Open-end
the system architecture
Optimal
the stages and incubators
Powerful
the ICS optics
Precise
the Harmonic Drive motor
Retrofittable
systems for micromanipulation
Sturdy
the mechanics
Versatile
5 filter positions with 23 mm
field of view
Vibration-free
the SoftStop function
Water-proof
the Aqua Stop
Zappy
the electric shutter
27
Axiovert 200 –
A Partnership for the Future
Ideally Suited for
That Extra Plus
the Perfect Handshake :
in Performance :
Zeiss Partners
Zeiss Service
Convincing from A – Z
the diaphragm planes
Complete
system solutions from a single
source
Whenever you need special soft-
The decision you make for a micro-
enable you to make great progress
ware for your applications, remem-
scope, one equipped with the
in your work. As a matter of fact,
Confocal
with LSM 510
the Light Trap
ber: Your partners are our part-
components you need, is as com-
you often can’t really appreciate
ners. Axiovert 200‘s open archi-
plex as the requirements it must
the full range of Zeiss service until
Contrastenhancing
tecture makes it easy for you to
meet. A skilled team of consul-
after you have made your pur-
Convenient
the Light Manager
effortlessly link software packages
tants will help you with making
chase – in practice, which is what
Customized
the modular system
AxioVisionControl software
from other suppliers. Which of
the right choice and with budget
counts. Our local consultants and
Digital
course makes Axiovert 200 the
planning. And all of our consul-
technicians and the Carl Zeiss cus-
Easy to use
the Push&Click filter cubes
the motorized features
ideal platform for a wide range of
tants possess impressive know-
tomer service all support you in
Efficient
special application driven software
how and experience as well as
your research with technical and
Ergonomic
the Ergotube
extensive knowledge of the entire
applications aid, whenever you
Flexible
the Dual Video Adapter
microscope market. Thus you ben-
need it. Fast and reliably. More-
Generous
efit from much more than our skill
over, Carl Zeiss training courses
the working distances of the
LD Condensers
in developing microscopes. You
and workshops provide you with
will be able to draw upon the
added insights into practical areas
enormous wealth of experience
of microscopy and imaging tech-
that Zeiss has accumulated in
niques. In fact, when you add to-
decades of practice in research and
gether all the services and support
routine. You will profit from con-
that you get with Axiovert 200,
crete assistance in your microscopy
you’ll see that it is more than a
methods – and above all from
high-power microscope:
innovative methods which will
Axiovert 200 is your ticket to
in many areas of research.
a powerhouse of knowledge in
microscopy that has been built up
over the past 150 years.
26
Accessible
Highly resolved DIC (even with Sénarmont)
Ideal
the documentation ports
Individual
the function buttons
Informative
the LCD display
N-dimensional
the modular Cell Observer
Open-end
the system architecture
Optimal
the stages and incubators
Powerful
the ICS optics
Precise
the Harmonic Drive motor
Retrofittable
systems for micromanipulation
Sturdy
the mechanics
Versatile
5 filter positions with 23 mm
field of view
Vibration-free
the SoftStop function
Water-proof
the Aqua Stop
Zappy
the electric shutter
27
14
Technical Data
Compare and Decide
Beam Path
15
2 eyepiece
3 intermediate image
plane frontport
16
2
1
Axiovert 200
Axiovert 200 M
Microscope stand
Highly stable pyramid shape and low center of gravity for vibration-free work,
min. 8 adaptation options (18 drilled holes) e.g. for micromanipulators
z-Focus
Harmonic Drive
Ratio Coarse: Fine = 50 :1
Optics
DIC System
13
6 sideport prisms
18
7 tube lens
Motorized Harmonic Drive,
minimum step size: 25 nm
Ratio Coarse: Fine =10 :1,
individually adjustable for every objective
8 analyzer
19
9 reflector module
10 luminous field diaphragm
11
10
11 aperture diaphragm
9
8
7
12
6 x objective nosepiece for DIC, motorized
Nomarski DIC – for every objective the optimal DIC slider,
contrast adjustment optional with Sénarmont with an analyzer and rotary λ/4 plate
12 filter slider
13 HBO lamp
3
14 HAL lamp
15 luminous field diaphragm
6
16 polarizer
17 aperture diaphragm
Illumination
Transmitted light
4 intermediate image
plane baseport
5 beam path switching
between baseport/
frontport/vis
17
ICS – Optics for best image quality
Special objectives with long working distance: LD A-Plan and LD Achroplan
6 x objective nosepiece for DIC, encoded
1 intermediate image
plane phototube
5
18 condenser
6 V, 30 W
19 objective
12 V, 100 W with on/off switch on the front of the stand, Köhler illumination
LCD display, Light Manager
Epi-fluorescence
Filter slider
4
Axiovert 200
3 positions for filters with 25 mm diameter
Diaphragm slider
Optional, centerable for aperture and luminous-field diaphragms for Köhler illumination in incident light
TV Ports
Reflector turret
5 positions, manual, exchangeable on both sides
Side port
Shutter, electric
Fast and easy-to-use shutter on the front of the stand
2 x left
100vis:0%doc / 20vis:80doc
Light Trap
Best signal to noise ratio, very good contrast, minimised stray light
3 x left
100vis:0%doc / 50vis:50doc / 0%vis:100%doc
The same like Axiovert 200, but motorized
Push&Click filter cube
Fast and precise change of filter cubes
3 x right
100vis:0%doc / 50vis:50doc / 0%vis:100%doc
The same like Axiovert 200, but motorized
Adjusting aid
Easy adjustment of the HBO burner
3 x right + left
100vis:0%doc/0vis:100doc left/20%vis:80%doc right
The same like Axiovert 200, but motorized
5 positions, manual or motorized, exchangeable
Front port
Tubes
Splitting:
Adapter for digital cameras, optional
Adapter for SLR cameras, optional
Binocular tube
With shutter to avoid stray light through overhead light
Phototube
With shutter and focusing Bertrand optics for easy adjustment of Ph apertures
Base port
Optional
Ergotube
With shutter and focusing Bertrand optics for easy adjustment of Ph apertures
Dual Video Adapter
For every interface 60, with two ports (one adjustable in x, y, z) and exchangeable beam splitter
Condenser
Eyepieces
PL 10 x/23
23 mm field of view. Budget model, sufficient if mainly cameras are used for observation
N.A. 0.35 W.D.* = 70 mm
For BF, Ph0, Ph1, Ph2, DIC
E-PL 10 x/23
23 mm field of view. For high-quality observation
N.A. 0.35 W.D.* = 70 mm
For BF/DIC, Ph0, Ph1, Ph2, VAR1
N.A. 0.55 W.D.* = 26 mm
For BF, Ph1, Ph2, Ph3, DIC, DIC, manual
Stages
28
Axiovert 200 M
Specimen stage (250x230mm)
With object guide for right and left, travel range: 130 x 85 mm with assorted frames
N.A. 0.55 W.D.* = 26 mm
For BF/DIC, Ph1, Ph2, Ph3, VAR1+2
Mechanical stage
Plane surface, large travel range: 130 x 85 mm with application-oriented specimen holders
N.A. 0.8 W.D.* = 7 mm
For BF/DIC, Ph1, Ph2, Ph3, D
Sliding stage (Z)
For precise and free positioning, especially in micromanipulation (e. g. for transgenic techniques)
N.A. 1.4 Oil Imm.
For BF, Ph, DIC
Scanning stage, motorized
Travel range: 120 x 100 mm with specimen holders (like mechanical stage)
Aqua Stop
For protection of nosepiece from spilled liquids
* with working distance
For BF, Ph1, Ph2, Ph3, DIC, DIC, motorized
29
14
Technical Data
Compare and Decide
Beam Path
15
2 eyepiece
3 intermediate image
plane frontport
16
2
1
Axiovert 200
Axiovert 200 M
Microscope stand
Highly stable pyramid shape and low center of gravity for vibration-free work,
min. 8 adaptation options (18 drilled holes) e.g. for micromanipulators
z-Focus
Harmonic Drive
Ratio Coarse: Fine = 50 :1
Optics
DIC System
13
6 sideport prisms
18
7 tube lens
Motorized Harmonic Drive,
minimum step size: 25 nm
Ratio Coarse: Fine =10 :1,
individually adjustable for every objective
8 analyzer
19
9 reflector module
10 luminous field diaphragm
11
10
11 aperture diaphragm
9
8
7
12
6 x objective nosepiece for DIC, motorized
Nomarski DIC – for every objective the optimal DIC slider,
contrast adjustment optional with Sénarmont with an analyzer and rotary λ/4 plate
12 filter slider
13 HBO lamp
3
14 HAL lamp
15 luminous field diaphragm
6
16 polarizer
17 aperture diaphragm
Illumination
Transmitted light
4 intermediate image
plane baseport
5 beam path switching
between baseport/
frontport/vis
17
ICS – Optics for best image quality
Special objectives with long working distance: LD A-Plan and LD Achroplan
6 x objective nosepiece for DIC, encoded
1 intermediate image
plane phototube
5
18 condenser
6 V, 30 W
19 objective
12 V, 100 W with on/off switch on the front of the stand, Köhler illumination
LCD display, Light Manager
Epi-fluorescence
Filter slider
4
Axiovert 200
3 positions for filters with 25 mm diameter
Diaphragm slider
Optional, centerable for aperture and luminous-field diaphragms for Köhler illumination in incident light
TV Ports
Reflector turret
5 positions, manual, exchangeable on both sides
Side port
Shutter, electric
Fast and easy-to-use shutter on the front of the stand
2 x left
100vis:0%doc / 20vis:80doc
Light Trap
Best signal to noise ratio, very good contrast, minimised stray light
3 x left
100vis:0%doc / 50vis:50doc / 0%vis:100%doc
The same like Axiovert 200, but motorized
Push&Click filter cube
Fast and precise change of filter cubes
3 x right
100vis:0%doc / 50vis:50doc / 0%vis:100%doc
The same like Axiovert 200, but motorized
Adjusting aid
Easy adjustment of the HBO burner
3 x right + left
100vis:0%doc/0vis:100doc left/20%vis:80%doc right
The same like Axiovert 200, but motorized
5 positions, manual or motorized, exchangeable
Front port
Tubes
Splitting:
Adapter for digital cameras, optional
Adapter for SLR cameras, optional
Binocular tube
With shutter to avoid stray light through overhead light
Phototube
With shutter and focusing Bertrand optics for easy adjustment of Ph apertures
Base port
Optional
Ergotube
With shutter and focusing Bertrand optics for easy adjustment of Ph apertures
Dual Video Adapter
For every interface 60, with two ports (one adjustable in x, y, z) and exchangeable beam splitter
Condenser
Eyepieces
PL 10 x/23
23 mm field of view. Budget model, sufficient if mainly cameras are used for observation
N.A. 0.35 W.D.* = 70 mm
For BF, Ph0, Ph1, Ph2, DIC
E-PL 10 x/23
23 mm field of view. For high-quality observation
N.A. 0.35 W.D.* = 70 mm
For BF/DIC, Ph0, Ph1, Ph2, VAR1
N.A. 0.55 W.D.* = 26 mm
For BF, Ph1, Ph2, Ph3, DIC, DIC, manual
Stages
28
Axiovert 200 M
Specimen stage (250x230mm)
With object guide for right and left, travel range: 130 x 85 mm with assorted frames
N.A. 0.55 W.D.* = 26 mm
For BF/DIC, Ph1, Ph2, Ph3, VAR1+2
Mechanical stage
Plane surface, large travel range: 130 x 85 mm with application-oriented specimen holders
N.A. 0.8 W.D.* = 7 mm
For BF/DIC, Ph1, Ph2, Ph3, D
Sliding stage (Z)
For precise and free positioning, especially in micromanipulation (e. g. for transgenic techniques)
N.A. 1.4 Oil Imm.
For BF, Ph, DIC
Scanning stage, motorized
Travel range: 120 x 100 mm with specimen holders (like mechanical stage)
Aqua Stop
For protection of nosepiece from spilled liquids
* with working distance
For BF, Ph1, Ph2, Ph3, DIC, DIC, motorized
29
AXIOBR_E.qxd
27.03.2001
14:18 Uhr
Seite 34
System Overview
Axiovert 200, Axiovert
Axiovert
200200M
DIC prism I/0.35
000000-1005-821
DICprism
prismI/0.55
I/0.55
DIC
000000-1005-866
000000-1005-866
DIC prism II/0.35
000000-1005-822
DICprism
prismII/0.55
II/0.55
DIC
000000-1005-867
000000-1005-867
Conversionfilter
filter
Conversion
3200-5500K,K,d=32x2
d=32x2
3200-5500
467847-0000-000
467847-0000-000
Interferencewide-band
wide-bandfilter
filter
Interference
green,d=32x4
d=32x4
green,
467803-0000-000
467803-0000-000
DICprism
prismIII/0.55
III/0.55
DIC
000000-1005-868
000000-1005-868
LCD
LCDdisplay
display
and
andlight
lightmanager
manager
000000-1071-167
000000-1071-167
100
100HAL
HALlamp
lamphousing
housingwith
withcollector,
collector,
lamp
lampmount
mount
447219-0000-000
447219-0000-000
12
12VV100
100W
WHalogen
Halogenlamp
lamp
380079-9540-000
380079-9540-000
Mounting frames for specimen stage with object guide
Mounting frame M for specimen slider 76 x 26
471719-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.22
0/0.22
0/0.22
451261-0000-000
451261-0000-000
451261-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var1/0.4
1/0.4
1/0.4
451251-0000-000
451251-0000-000
451251-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.2
1/0.2
1/0.2
451260-0000-000
451260-0000-000
451260-0000-000
Diaphragm
Diaphragm
Diaphragmvar
var
var1/0.55
1/0.55
1/0.55
451252-0000-000
451252-0000-000
451252-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.4
0/0.4
0/0.4
451255-0000-000
451255-0000-000
451255-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var2/0.55
2/0.55
2/0.55
451253-0000-000
451253-0000-000
451253-0000-000
44
Mounting frame M for Petri dishes, dia. 36 mm
471742-0000-000
Mounting frame M for Petri dishes, dia. 54 mm
471743-0000-000
Mounting frame M for Petri dishes, dia. 65 mm
471758-0000-000
Mounting frame M for Petri dishes, dia. 88 mm
471759-0000-000
Simple gliding stage
with stage inserts d=24 and 48mm
471720-0000-000
Gliding stage Z
with stage inserts d=24 and 48mm
471722-0000-000
LD condenser 0.35 H, Ph 0, Ph 1, Ph 2, DIC, DIC
000000-1005-845
DIC
DICprism
prismIII/0.8
III/0.8
445485-0000-000
445485-0000-000
DIC
DICprism
prismII/1.4
II/1.4
445375-0000-000
445375-0000-000
Achromatic condenser 0.8 H, DIC
000000-1087-444
DIC prism III/0.8
000000-1087-445
6
StSt
anan Stan
dbdb db
yy
y
32
0000 00
KK K
7
32
832
10
12
ExEx Ex
tete
rnrn tern
Slider
Slider
SliderPh,
Ph,
Ph,H,
H,
H,Var
Var
Var
451246-0000-000
451246-0000-000
451246-0000-000
Power
Power
Powersupply
supply
supply12V
12V
12VDC
DC
DC100W
100W
100Wstabilized
stabilized
stabilized
000000-1128-574
000000-1128-574
000000-1128-574
Carrier
Carrier
Carrierfor
for
for6V
6V
6V25/30W
25/30W
25/30W
transmitted-light
transmitted-light
transmitted-lightillumination
illumination
illumination
451380-0000-000
451380-0000-000
451380-0000-000
Slider
SliderPh,
Ph,H,
H,Ph
Ph
Slider
Ph,
H,
Ph
451245-0000-000
451245-0000-000
451245-0000-000
LALA
LA
MPMP
MP
Condenser
Condenser
Condenser0.2
0.2
0.2
451236-0000-000
451236-0000-000
451236-0000-000
TETE
TE
MP
MPMP
NENE
NE
eebb eb
POPOTZTZ PO TZ
WEWE
WE
RR
R
qq q
1100 10
00 0
ddcc dc
Condenser
Condenser
Condenser0.4
0.4
0.4
451235-0000-000
451235-0000-000
451235-0000-000
Adapter
Adapterfor
forcarrier
carrierfor
for30W
30W
Adapter
for
carrier
for
30W
transmitted-light
transmitted-lightillumination
illumination
transmitted-light
illumination
000000-1005-842
000000-1005-842
000000-1005-842
Power
Powersupply
supplyunit
unitfor
forN
HBO103
103
Power
supply
unit
for
NNHBO
HBO
103
90...250V,
90...250V,50...60Hz,
50...60Hz,265
265VA
VA
90...250V,
50...60Hz,
265
VA
000000-1003-928
000000-1003-928
000000-1003-928
ControlPC-board
PC-board
Control
000000-1015-348
000000-1015-348
Achromatic-aplanaticcondenser
condenser
Achromatic-aplanatic
1.4H,
H,D,
D,Ph,
Ph,DIC
DIC
1.4
445453-0000-000
445453-0000-000
Universal mounting frame M-X
471717-0000-000
77
88
Condenser
Condenser
Condenser0.55
0.55
0.55
451237-0000-000
451237-0000-000
451237-0000-000
Achromaticcondenser
condenser
Achromatic
0.8H,
H,D,
D,Ph,
Ph,DIC
DIC
0.8
445445-9901-000
445445-9901-000
5
66
Condenser
Condensermount
mountfor
for
condensers
condensers0.8/1.4
0.8/1.4
000000-1005-849
000000-1005-849
DIC
DICprism
prismIII/1.4
III/1.4
445376-0000-000
445376-0000-000
Mounting frame M for microtiter plates 96 positions
471746-0000-000
Mounting frame M for multitest dishes
471744-0000-000
Carrier
Carrierfor
fortransmitted-light
transmitted-lightillumination
illumination
and
andcondenser
condenser
000000-1005-840
000000-1005-840
PolarizerD,
D,rotatable
rotatable
Polarizer
000000-1121-813
000000-1121-813
4
55
1010
1212
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph2/0.55
2/0.55
2/0.55
451259-0000-000
451259-0000-000
451259-0000-000
Polarizer
PolarizerDD
000000-1005-864
000000-1005-864
LD
LDcondenser
condenser0.55
0.55H/DIC,
H/DIC,Ph
Ph1,1,Ph
Ph2,2,Ph
Ph3,3,Var
Var1/2
1/2
000000-1005-846
000000-1005-846
LD
LDcondenser
condenser0.55
0.55H,
H,Ph
Ph1,1,Ph
Ph2,2,Ph
Ph3,3,DIC,
DIC,DIC
DIC
000000-1005-847
000000-1005-847
O
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.4
1/0.4
1/0.4
451256-0000-000
451256-0000-000
451256-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.55
1/0.55
1/0.55
451258-0000-000
451258-0000-000
451258-0000-000
LD condenser 0.35 H/DIC, Ph 0, Ph 1, Ph 2, Var 1
000000-1005-844
SSNNSN
TT11T 1
22VV2V
1100 10
I
00WW0W
II
OO
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph2/0.4
2/0.4
2/0.4
451257-0000-000
451257-0000-000
451257-0000-000
Mounting frame M for microtest plates
471747-0000-000
for
for
forexternal
external
externalpower
power
powersupply
supply
supplyHAL
HAL
HAL
Adjusting
Adjustingaid
aidfor
forHBO/XBO
HBO/XBO
Adjusting
aid
for
HBO/XBO
452369-0000-000
452369-0000-000
452369-0000-000
000
1100
10
OOO
HHBB
HB
Universal mounting frame M
471718-0000-000
SaSa
Sa
fefe
fe
tyty
ty
tt t
On
On On
Po
Po Po Of
we weOfff Off
we
rr r
HHBBHtBtooAtAoA
OO O rrccrc
110010 22 2
0W
00W
W
tttttt
ooAAoA
rrccrc
CarCar
Car
l l
l
ZeiZei
Zei
ss ss
ss
LaLa
La
mpmp Onmp
OnOn
++
IgIg
Ig
ni
nini
te
tete
LevLev
1 1el el
Universal mounting frame M-Duo
000000-1116-077
Object mount for two Petri dishes "35"
or one object carrier (PC)
000000-1116-088
TV-adapter
acc. price list
Cover
Coverglass
glasscup
cup
000000-1016-757
000000-1016-757
Objectives
Objectives
acc.
acc.price
pricelist
list
AquaStop
Stop
Aqua
000000-1109-740
000000-1109-740
Antiglarescreen
screenfor
for"Axiovert"
"Axiovert"200
200
Antiglare
000000-1109-741
000000-1109-741
Path-deflecting
Path-deflecting
Path-deflectingmirror
mirror
mirror
for
for
fortwo
two
twoilluminators
illuminators
illuminators
447230-9901-000
447230-9901-000
447230-9901-000
ControlPC-board
PC-boardwith
withinterface
interface
Control
000000-1015-381
000000-1015-381
Auxiliary microscope d=30
000000-1006-362
Analyzer
Analyzerslider,
slider,fixed
fixed
000000-1005-862
000000-1005-862
Object guide 130x85 mm right
000000-1005-833
Eyepiece eyecup
444801-0000-000
FL
FL
FLincident-light
incident-light
incident-lightequipment
equipment
equipment
000000-1066-230
000000-1066-230
000000-1066-230
Illuminator
IlluminatorN
HBO103
103
Illuminator
NNHBO
HBO
103
000000-1007-980
000000-1007-980
000000-1007-980
Collector
CollectorN
HBO103/XBO
103/XBO75
75
Collector
NNHBO
HBO
103/XBO
75
000000-1007-976
000000-1007-976
000000-1007-976
Achromatic
Achromaticcollector
collector
Achromatic
collector
000000-1007-978
000000-1007-978
000000-1007-978
Mercury
Mercuryvapor
vaporshort-arc
short-arclamp
lampHBO
HBO103
103W/2
W/2
Mercury
vapor
short-arc
lamp
HBO
103
W/2
380301-9350-000
380301-9350-000
380301-9350-000
--
Lev
1 el
LevLev
2 2el el
TiTi
Ti
meme Reme
ReRe
r r ser
sese
tt
t
+
-
Lev
2 el
HouHou
rs rs
Hou
rs
Variable
Variableintensity
intensitylamp
lampcontrol
controlAttoArc
AttoArc222N
HBO
Variable
intensity
lamp
control
AttoArc
NNHBO
HBO
000000-1007-975
000000-1007-975
000000-1007-975
Iris
Irisstop
stopslider
sliderfor
for
Iris
stop
slider
for
incident-light
incident-lightequipment
equipment
incident-light
equipment
000000-1062-360
000000-1062-360
000000-1062-360
HOU
HOU
RSRS
HOU
RS
230
230
POW
POW
POW
ER
ERER
CUR
CUR
CUR
TIS
TIS
TIS
230
LAM
LAM
P P
LAM
P
SAF
SAF
SAF
ETY
ETY
ETY
7755
75
OOO
XXBB
XB
Cable
Cablerelease
release
416167-0000-000
416167-0000-000
Object guide 130x85 mm left
000000-1110-991
Axiovert200
200basic
basicstand
stand
Axiovert
000000-1005-813
000000-1005-813
Binocular phototube
with sliding prism 45°/23
000000-1005-828
Eyepiece E-PL 10x20 Br.
444231-9901-000
Eyepiece E-PL 10x20 Br. foc
4444232-9902-000
Eyepiece W-PL 10x23 Br. foc
000000-1016-758
Mounting frames for mechanical stage
Mounting frame K
451341-0000-000
Binocular tube 45°/23
000000-1005-827
Universal mounting frame K-X
451353-0000-000
Mechanical stage 130x85 R/L
without mounting frames
000000-1005-834
Mounting frame K, d=72mm
451344-0000-000
Stage stop d=28 mm
451345-0000-000
30
HighResolution
ResolutionMicroscopy
MicroscopyCamera
CameraAxioCam
AxioCamb/w
b/w
High
000000-0412-311
000000-0412-311
HighResolution
ResolutionMicroscopy
MicroscopyColour
ColourCamera
CameraAxioCam
AxioCamColour
Colour
High
000000-0412-312
000000-0412-312
Universal mounting frame K-Duo
000000-1116-078
Binocular ergotube 25°/23
000000-1005-829
Pathdeflection
deflectionto
tothe
thetube
tube
Path
000000-1069-227
000000-1069-227
Beampath
pathswitching
switching
Beam
000000-1069-228
000000-1069-228
P
Illuminator
IlluminatorN
XBO75
75
Illuminator
NNXBO
XBO
75
000000-1007-981
000000-1007-981
000000-1007-981
Collector
CollectorN
HBO103/XBO
103/XBO75
75
Collector
NNHBO
HBO
103/XBO
75
000000-1007-976
000000-1007-976
000000-1007-976
Achromatic
Achromaticcollector
collector
Achromatic
collector
000000-1007-978
000000-1007-978
000000-1007-978
Xenon
Xenonlamp
lampXBO
XBO75
75W/2
W/2
Xenon
lamp
XBO
75
W/2
380053-9870-000
380053-9870-000
380053-9870-000
Protective
Protectivegloves
gloves
Protective
gloves
417008-0000-000
417008-0000-000
417008-0000-000
Face
Faceprotection
protectionshield
shield
Face
protection
shield
417009-0000-000
417009-0000-000
417009-0000-000
Reflector
Reflector
Reflectormodule
module
modulePol
Pol
PolP&C
P&C
P&C
000000-1046-279
000000-1046-279
000000-1046-279
Reflector
Reflector
Reflectormodule
module
moduleFl
FlFlP&C
P&C
P&C
000000-1046-281
000000-1046-281
000000-1046-281
Analyzer
Analyzer
Analyzermodule
module
moduleD
DDP&C
P&C
P&C
000000-1050-958
000000-1050-958
000000-1050-958
Baseport
Baseport60
60
000000-1005-824
000000-1005-824
AdapterVideo
VideoV200
V200CC2/3"
2/3"0.63x
0.63x
Adapter
frontport
atatfrontport
000000-1071-171
000000-1071-171
Universal mounting frame K
451352-0000-000
1:1
T2-adapter
T2-adapterfor
forContax
Contax
(Contax-Bayonet)
(Contax-Bayonet)
416010-0000-000
416010-0000-000
TEM
Power
Powersupply
supplyunit
unitfor
forN
XBO75
75
Power
supply
unit
for
NNXBO
XBO
75
90...250V,
90...250V,50...60Hz,
50...60Hz,130
130VA
VA
90...250V,
50...60Hz,
130
VA
000000-1003-924
000000-1003-924
000000-1003-924
Protection
Protection
Protectionfilter
filter
filterfor
for
forreflector
reflector
reflectorturret
turret
turret
000000-1068-113
000000-1068-113
000000-1068-113
5-position
5-position
5-positionreflector
reflector
reflectorturret,
turret,
turret,empty
empty
empty
000000-1064-364
000000-1064-364
000000-1064-364
Optovar
Optovar1.6x
1.6x
000000-1005-855
000000-1005-855
Optovar
Optovar2.5x
2.5x
000000-1005-856
000000-1005-856
m
Cam
i oCa
A xi o
Ax
Specimen stage 250x230 mm
000000-1005-832
Single-lens
Single-lensreflex
reflexcamera
camerahousing
housing
Contax
ContaxAria
Aria(D)
(D)
000000-1057-127
000000-1057-127
DIC
DICslider
slider
acc.
acc.price
pricelist
list
Adapter
AdapterV200
V200T2
T22.5x
2.5xfor
forSLR
SLR
atatfrontport
frontport
000000-1005-869
000000-1005-869
Analyzer
Analyzerslider
slider+/+/-30°
30°
000000-1005-863
000000-1005-863
TEM
TEM
P P
Sideport
Sideport60
60left,
left,22switching
switchingpositions
positions
000000-1005-815
000000-1005-815
Sideport
Sideport60
60left,
left,33switching
switchingpositions
positions
000000-1005-816
000000-1005-816
Sideport
Sideport60
60right,
right,33switching
switchingpositions
positions
000000-1005-817
000000-1005-817
Sideport
Sideport60
60left
leftand
andright,
right,33switching
switchingpositions
positions
000000-1005-818
000000-1005-818
Doublevideo
videoadapter
adapter
Double
000000-1058-640
000000-1058-640
31
Lamp
Lamp
Lamphousing
housing
housingHBO
HBO
HBO50
50
50with
with
withsocket
socket
socket
447220-0000-000
447220-0000-000
447220-0000-000
Lamp
Lamp
Lampcollektor
collektor
collektorHBO
HBO
HBO50/SF
50/SF
50/SF25
25
25
447270-0000-000
447270-0000-000
447270-0000-000
HBO
HBO
HBO50
50
50super-pressure
super-pressure
super-pressuremercury
mercury
mercurylamp
lamp
lamp
381619-0000-000
381619-0000-000
381619-0000-000
Power
Power
Powersupply
supply
supplyunit
unit
unitfor
for
forHBO
HBO
HBO50,
50,
50,
100...240
100...240
100...240V,
V,V,50...60
50...60
50...60Hz,
Hz,
Hz,350VA
350VA
350VA
000000-1113-833
000000-1113-833
000000-1113-833
32
AXIOBR_E.qxd
27.03.2001
14:18 Uhr
Seite 34
System Overview
Axiovert 200, Axiovert
Axiovert
200200M
DIC prism I/0.35
000000-1005-821
DICprism
prismI/0.55
I/0.55
DIC
000000-1005-866
000000-1005-866
DIC prism II/0.35
000000-1005-822
DICprism
prismII/0.55
II/0.55
DIC
000000-1005-867
000000-1005-867
Conversionfilter
filter
Conversion
3200-5500K,K,d=32x2
d=32x2
3200-5500
467847-0000-000
467847-0000-000
Interferencewide-band
wide-bandfilter
filter
Interference
green,d=32x4
d=32x4
green,
467803-0000-000
467803-0000-000
DICprism
prismIII/0.55
III/0.55
DIC
000000-1005-868
000000-1005-868
LCD
LCDdisplay
display
and
andlight
lightmanager
manager
000000-1071-167
000000-1071-167
100
100HAL
HALlamp
lamphousing
housingwith
withcollector,
collector,
lamp
lampmount
mount
447219-0000-000
447219-0000-000
12
12VV100
100W
WHalogen
Halogenlamp
lamp
380079-9540-000
380079-9540-000
Mounting frames for specimen stage with object guide
Mounting frame M for specimen slider 76 x 26
471719-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.22
0/0.22
0/0.22
451261-0000-000
451261-0000-000
451261-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var1/0.4
1/0.4
1/0.4
451251-0000-000
451251-0000-000
451251-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.2
1/0.2
1/0.2
451260-0000-000
451260-0000-000
451260-0000-000
Diaphragm
Diaphragm
Diaphragmvar
var
var1/0.55
1/0.55
1/0.55
451252-0000-000
451252-0000-000
451252-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.4
0/0.4
0/0.4
451255-0000-000
451255-0000-000
451255-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var2/0.55
2/0.55
2/0.55
451253-0000-000
451253-0000-000
451253-0000-000
44
Mounting frame M for Petri dishes, dia. 36 mm
471742-0000-000
Mounting frame M for Petri dishes, dia. 54 mm
471743-0000-000
Mounting frame M for Petri dishes, dia. 65 mm
471758-0000-000
Mounting frame M for Petri dishes, dia. 88 mm
471759-0000-000
Simple gliding stage
with stage inserts d=24 and 48mm
471720-0000-000
Gliding stage Z
with stage inserts d=24 and 48mm
471722-0000-000
LD condenser 0.35 H, Ph 0, Ph 1, Ph 2, DIC, DIC
000000-1005-845
DIC
DICprism
prismIII/0.8
III/0.8
445485-0000-000
445485-0000-000
DIC
DICprism
prismII/1.4
II/1.4
445375-0000-000
445375-0000-000
Achromatic condenser 0.8 H, DIC
000000-1087-444
DIC prism III/0.8
000000-1087-445
6
StSt
anan Stan
dbdb db
yy
y
32
0000 00
KK K
7
32
832
10
12
ExEx Ex
tete
rnrn tern
Slider
Slider
SliderPh,
Ph,
Ph,H,
H,
H,Var
Var
Var
451246-0000-000
451246-0000-000
451246-0000-000
Power
Power
Powersupply
supply
supply12V
12V
12VDC
DC
DC100W
100W
100Wstabilized
stabilized
stabilized
000000-1128-574
000000-1128-574
000000-1128-574
Carrier
Carrier
Carrierfor
for
for6V
6V
6V25/30W
25/30W
25/30W
transmitted-light
transmitted-light
transmitted-lightillumination
illumination
illumination
451380-0000-000
451380-0000-000
451380-0000-000
Slider
SliderPh,
Ph,H,
H,Ph
Ph
Slider
Ph,
H,
Ph
451245-0000-000
451245-0000-000
451245-0000-000
LALA
LA
MPMP
MP
Condenser
Condenser
Condenser0.2
0.2
0.2
451236-0000-000
451236-0000-000
451236-0000-000
TETE
TE
MP
MPMP
NENE
NE
eebb eb
POPOTZTZ PO TZ
WEWE
WE
RR
R
qq q
1100 10
00 0
ddcc dc
Condenser
Condenser
Condenser0.4
0.4
0.4
451235-0000-000
451235-0000-000
451235-0000-000
Adapter
Adapterfor
forcarrier
carrierfor
for30W
30W
Adapter
for
carrier
for
30W
transmitted-light
transmitted-lightillumination
illumination
transmitted-light
illumination
000000-1005-842
000000-1005-842
000000-1005-842
Power
Powersupply
supplyunit
unitfor
forN
HBO103
103
Power
supply
unit
for
NNHBO
HBO
103
90...250V,
90...250V,50...60Hz,
50...60Hz,265
265VA
VA
90...250V,
50...60Hz,
265
VA
000000-1003-928
000000-1003-928
000000-1003-928
ControlPC-board
PC-board
Control
000000-1015-348
000000-1015-348
Achromatic-aplanaticcondenser
condenser
Achromatic-aplanatic
1.4H,
H,D,
D,Ph,
Ph,DIC
DIC
1.4
445453-0000-000
445453-0000-000
Universal mounting frame M-X
471717-0000-000
77
88
Condenser
Condenser
Condenser0.55
0.55
0.55
451237-0000-000
451237-0000-000
451237-0000-000
Achromaticcondenser
condenser
Achromatic
0.8H,
H,D,
D,Ph,
Ph,DIC
DIC
0.8
445445-9901-000
445445-9901-000
5
66
Condenser
Condensermount
mountfor
for
condensers
condensers0.8/1.4
0.8/1.4
000000-1005-849
000000-1005-849
DIC
DICprism
prismIII/1.4
III/1.4
445376-0000-000
445376-0000-000
Mounting frame M for microtiter plates 96 positions
471746-0000-000
Mounting frame M for multitest dishes
471744-0000-000
Carrier
Carrierfor
fortransmitted-light
transmitted-lightillumination
illumination
and
andcondenser
condenser
000000-1005-840
000000-1005-840
PolarizerD,
D,rotatable
rotatable
Polarizer
000000-1121-813
000000-1121-813
4
55
1010
1212
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph2/0.55
2/0.55
2/0.55
451259-0000-000
451259-0000-000
451259-0000-000
Polarizer
PolarizerDD
000000-1005-864
000000-1005-864
LD
LDcondenser
condenser0.55
0.55H/DIC,
H/DIC,Ph
Ph1,1,Ph
Ph2,2,Ph
Ph3,3,Var
Var1/2
1/2
000000-1005-846
000000-1005-846
LD
LDcondenser
condenser0.55
0.55H,
H,Ph
Ph1,1,Ph
Ph2,2,Ph
Ph3,3,DIC,
DIC,DIC
DIC
000000-1005-847
000000-1005-847
O
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.4
1/0.4
1/0.4
451256-0000-000
451256-0000-000
451256-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.55
1/0.55
1/0.55
451258-0000-000
451258-0000-000
451258-0000-000
LD condenser 0.35 H/DIC, Ph 0, Ph 1, Ph 2, Var 1
000000-1005-844
SSNNSN
TT11T 1
22VV2V
1100 10
I
00WW0W
II
OO
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph2/0.4
2/0.4
2/0.4
451257-0000-000
451257-0000-000
451257-0000-000
Mounting frame M for microtest plates
471747-0000-000
for
for
forexternal
external
externalpower
power
powersupply
supply
supplyHAL
HAL
HAL
Adjusting
Adjustingaid
aidfor
forHBO/XBO
HBO/XBO
Adjusting
aid
for
HBO/XBO
452369-0000-000
452369-0000-000
452369-0000-000
000
1100
10
OOO
HHBB
HB
Universal mounting frame M
471718-0000-000
SaSa
Sa
fefe
fe
tyty
ty
tt t
On
On On
Po
Po Po Of
we weOfff Off
we
rr r
HHBBHtBtooAtAoA
OO O rrccrc
110010 22 2
0W
00W
W
tttttt
ooAAoA
rrccrc
CarCar
Car
l l
l
ZeiZei
Zei
ss ss
ss
LaLa
La
mpmp Onmp
OnOn
++
IgIg
Ig
ni
nini
te
tete
LevLev
1 1el el
Universal mounting frame M-Duo
000000-1116-077
Object mount for two Petri dishes "35"
or one object carrier (PC)
000000-1116-088
TV-adapter
acc. price list
Cover
Coverglass
glasscup
cup
000000-1016-757
000000-1016-757
Objectives
Objectives
acc.
acc.price
pricelist
list
AquaStop
Stop
Aqua
000000-1109-740
000000-1109-740
Antiglarescreen
screenfor
for"Axiovert"
"Axiovert"200
200
Antiglare
000000-1109-741
000000-1109-741
Path-deflecting
Path-deflecting
Path-deflectingmirror
mirror
mirror
for
for
fortwo
two
twoilluminators
illuminators
illuminators
447230-9901-000
447230-9901-000
447230-9901-000
ControlPC-board
PC-boardwith
withinterface
interface
Control
000000-1015-381
000000-1015-381
Auxiliary microscope d=30
000000-1006-362
Analyzer
Analyzerslider,
slider,fixed
fixed
000000-1005-862
000000-1005-862
Object guide 130x85 mm right
000000-1005-833
Eyepiece eyecup
444801-0000-000
FL
FL
FLincident-light
incident-light
incident-lightequipment
equipment
equipment
000000-1066-230
000000-1066-230
000000-1066-230
Illuminator
IlluminatorN
HBO103
103
Illuminator
NNHBO
HBO
103
000000-1007-980
000000-1007-980
000000-1007-980
Collector
CollectorN
HBO103/XBO
103/XBO75
75
Collector
NNHBO
HBO
103/XBO
75
000000-1007-976
000000-1007-976
000000-1007-976
Achromatic
Achromaticcollector
collector
Achromatic
collector
000000-1007-978
000000-1007-978
000000-1007-978
Mercury
Mercuryvapor
vaporshort-arc
short-arclamp
lampHBO
HBO103
103W/2
W/2
Mercury
vapor
short-arc
lamp
HBO
103
W/2
380301-9350-000
380301-9350-000
380301-9350-000
--
Lev
1 el
LevLev
2 2el el
TiTi
Ti
meme Reme
ReRe
r r ser
sese
tt
t
+
-
Lev
2 el
HouHou
rs rs
Hou
rs
Variable
Variableintensity
intensitylamp
lampcontrol
controlAttoArc
AttoArc222N
HBO
Variable
intensity
lamp
control
AttoArc
NNHBO
HBO
000000-1007-975
000000-1007-975
000000-1007-975
Iris
Irisstop
stopslider
sliderfor
for
Iris
stop
slider
for
incident-light
incident-lightequipment
equipment
incident-light
equipment
000000-1062-360
000000-1062-360
000000-1062-360
HOU
HOU
RSRS
HOU
RS
230
230
POW
POW
POW
ER
ERER
CUR
CUR
CUR
TIS
TIS
TIS
230
LAM
LAM
P P
LAM
P
SAF
SAF
SAF
ETY
ETY
ETY
7755
75
OOO
XXBB
XB
Cable
Cablerelease
release
416167-0000-000
416167-0000-000
Object guide 130x85 mm left
000000-1110-991
Axiovert200
200basic
basicstand
stand
Axiovert
000000-1005-813
000000-1005-813
Binocular phototube
with sliding prism 45°/23
000000-1005-828
Eyepiece E-PL 10x20 Br.
444231-9901-000
Eyepiece E-PL 10x20 Br. foc
4444232-9902-000
Eyepiece W-PL 10x23 Br. foc
000000-1016-758
Mounting frames for mechanical stage
Mounting frame K
451341-0000-000
Binocular tube 45°/23
000000-1005-827
Universal mounting frame K-X
451353-0000-000
Mechanical stage 130x85 R/L
without mounting frames
000000-1005-834
Mounting frame K, d=72mm
451344-0000-000
Stage stop d=28 mm
451345-0000-000
30
HighResolution
ResolutionMicroscopy
MicroscopyCamera
CameraAxioCam
AxioCamb/w
b/w
High
000000-0412-311
000000-0412-311
HighResolution
ResolutionMicroscopy
MicroscopyColour
ColourCamera
CameraAxioCam
AxioCamColour
Colour
High
000000-0412-312
000000-0412-312
Universal mounting frame K-Duo
000000-1116-078
Binocular ergotube 25°/23
000000-1005-829
Pathdeflection
deflectionto
tothe
thetube
tube
Path
000000-1069-227
000000-1069-227
Beampath
pathswitching
switching
Beam
000000-1069-228
000000-1069-228
P
Illuminator
IlluminatorN
XBO75
75
Illuminator
NNXBO
XBO
75
000000-1007-981
000000-1007-981
000000-1007-981
Collector
CollectorN
HBO103/XBO
103/XBO75
75
Collector
NNHBO
HBO
103/XBO
75
000000-1007-976
000000-1007-976
000000-1007-976
Achromatic
Achromaticcollector
collector
Achromatic
collector
000000-1007-978
000000-1007-978
000000-1007-978
Xenon
Xenonlamp
lampXBO
XBO75
75W/2
W/2
Xenon
lamp
XBO
75
W/2
380053-9870-000
380053-9870-000
380053-9870-000
Protective
Protectivegloves
gloves
Protective
gloves
417008-0000-000
417008-0000-000
417008-0000-000
Face
Faceprotection
protectionshield
shield
Face
protection
shield
417009-0000-000
417009-0000-000
417009-0000-000
Reflector
Reflector
Reflectormodule
module
modulePol
Pol
PolP&C
P&C
P&C
000000-1046-279
000000-1046-279
000000-1046-279
Reflector
Reflector
Reflectormodule
module
moduleFl
FlFlP&C
P&C
P&C
000000-1046-281
000000-1046-281
000000-1046-281
Analyzer
Analyzer
Analyzermodule
module
moduleD
DDP&C
P&C
P&C
000000-1050-958
000000-1050-958
000000-1050-958
Baseport
Baseport60
60
000000-1005-824
000000-1005-824
AdapterVideo
VideoV200
V200CC2/3"
2/3"0.63x
0.63x
Adapter
frontport
atatfrontport
000000-1071-171
000000-1071-171
Universal mounting frame K
451352-0000-000
1:1
T2-adapter
T2-adapterfor
forContax
Contax
(Contax-Bayonet)
(Contax-Bayonet)
416010-0000-000
416010-0000-000
TEM
Power
Powersupply
supplyunit
unitfor
forN
XBO75
75
Power
supply
unit
for
NNXBO
XBO
75
90...250V,
90...250V,50...60Hz,
50...60Hz,130
130VA
VA
90...250V,
50...60Hz,
130
VA
000000-1003-924
000000-1003-924
000000-1003-924
Protection
Protection
Protectionfilter
filter
filterfor
for
forreflector
reflector
reflectorturret
turret
turret
000000-1068-113
000000-1068-113
000000-1068-113
5-position
5-position
5-positionreflector
reflector
reflectorturret,
turret,
turret,empty
empty
empty
000000-1064-364
000000-1064-364
000000-1064-364
Optovar
Optovar1.6x
1.6x
000000-1005-855
000000-1005-855
Optovar
Optovar2.5x
2.5x
000000-1005-856
000000-1005-856
m
Cam
i oCa
A xi o
Ax
Specimen stage 250x230 mm
000000-1005-832
Single-lens
Single-lensreflex
reflexcamera
camerahousing
housing
Contax
ContaxAria
Aria(D)
(D)
000000-1057-127
000000-1057-127
DIC
DICslider
slider
acc.
acc.price
pricelist
list
Adapter
AdapterV200
V200T2
T22.5x
2.5xfor
forSLR
SLR
atatfrontport
frontport
000000-1005-869
000000-1005-869
Analyzer
Analyzerslider
slider+/+/-30°
30°
000000-1005-863
000000-1005-863
TEM
TEM
P P
Sideport
Sideport60
60left,
left,22switching
switchingpositions
positions
000000-1005-815
000000-1005-815
Sideport
Sideport60
60left,
left,33switching
switchingpositions
positions
000000-1005-816
000000-1005-816
Sideport
Sideport60
60right,
right,33switching
switchingpositions
positions
000000-1005-817
000000-1005-817
Sideport
Sideport60
60left
leftand
andright,
right,33switching
switchingpositions
positions
000000-1005-818
000000-1005-818
Doublevideo
videoadapter
adapter
Double
000000-1058-640
000000-1058-640
31
Lamp
Lamp
Lamphousing
housing
housingHBO
HBO
HBO50
50
50with
with
withsocket
socket
socket
447220-0000-000
447220-0000-000
447220-0000-000
Lamp
Lamp
Lampcollektor
collektor
collektorHBO
HBO
HBO50/SF
50/SF
50/SF25
25
25
447270-0000-000
447270-0000-000
447270-0000-000
HBO
HBO
HBO50
50
50super-pressure
super-pressure
super-pressuremercury
mercury
mercurylamp
lamp
lamp
381619-0000-000
381619-0000-000
381619-0000-000
Power
Power
Powersupply
supply
supplyunit
unit
unitfor
for
forHBO
HBO
HBO50,
50,
50,
100...240
100...240
100...240V,
V,V,50...60
50...60
50...60Hz,
Hz,
Hz,350VA
350VA
350VA
000000-1113-833
000000-1113-833
000000-1113-833
32
AXIOBR_E.qxd
27.03.2001
14:18 Uhr
Seite 34
System Overview
Axiovert 200, Axiovert
Axiovert
200200M
DIC prism I/0.35
000000-1005-821
DICprism
prismI/0.55
I/0.55
DIC
000000-1005-866
000000-1005-866
DIC prism II/0.35
000000-1005-822
DICprism
prismII/0.55
II/0.55
DIC
000000-1005-867
000000-1005-867
Conversionfilter
filter
Conversion
3200-5500K,K,d=32x2
d=32x2
3200-5500
467847-0000-000
467847-0000-000
Interferencewide-band
wide-bandfilter
filter
Interference
green,d=32x4
d=32x4
green,
467803-0000-000
467803-0000-000
DICprism
prismIII/0.55
III/0.55
DIC
000000-1005-868
000000-1005-868
LCD
LCDdisplay
display
and
andlight
lightmanager
manager
000000-1071-167
000000-1071-167
100
100HAL
HALlamp
lamphousing
housingwith
withcollector,
collector,
lamp
lampmount
mount
447219-0000-000
447219-0000-000
12
12VV100
100W
WHalogen
Halogenlamp
lamp
380079-9540-000
380079-9540-000
Mounting frames for specimen stage with object guide
Mounting frame M for specimen slider 76 x 26
471719-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.22
0/0.22
0/0.22
451261-0000-000
451261-0000-000
451261-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var1/0.4
1/0.4
1/0.4
451251-0000-000
451251-0000-000
451251-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph1/0.2
1/0.2
1/0.2
451260-0000-000
451260-0000-000
451260-0000-000
Diaphragm
Diaphragm
Diaphragmvar
var
var1/0.55
1/0.55
1/0.55
451252-0000-000
451252-0000-000
451252-0000-000
Phase
Phase
Phasestop
stop
stopPh
Ph
Ph0/0.4
0/0.4
0/0.4
451255-0000-000
451255-0000-000
451255-0000-000
Diaphragm
Diaphragm
DiaphragmVar
Var
Var2/0.55
2/0.55
2/0.55
451253-0000-000
451253-0000-000
451253-0000-000
44
Mounting frame M for Petri dishes, dia. 36 mm
471742-0000-000
Mounting frame M for Petri dishes, dia. 54 mm
471743-0000-000
Mounting frame M for Petri dishes, dia. 65 mm
471758-0000-000
Mounting frame M for Petri dishes, dia. 88 mm
471759-0000-000
Simple gliding stage
with stage inserts d=24 and 48mm
471720-0000-000
Gliding stage Z
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y
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32
832
10
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ExEx Ex
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Adapter
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77
88
Condenser
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451237-0000-000
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Carrier
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Ph
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1/0.55
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LD condenser 0.35 H/DIC, Ph 0, Ph 1, Ph 2, Var 1
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SSNNSN
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OO
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Mounting frame M for microtest plates
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Sa
fefe
fe
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ty
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Po Po Of
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rr r
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380301-9350-000
380301-9350-000
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--
Lev
1 el
LevLev
2 2el el
TiTi
Ti
meme Reme
ReRe
r r ser
sese
tt
t
+
-
Lev
2 el
HouHou
rs rs
Hou
rs
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HBO
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HBO
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000000-1007-975
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000000-1062-360
000000-1062-360
HOU
HOU
RSRS
HOU
RS
230
230
POW
POW
POW
ER
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CUR
CUR
CUR
TIS
TIS
TIS
230
LAM
LAM
P P
LAM
P
SAF
SAF
SAF
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ETY
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7755
75
OOO
XXBB
XB
Cable
Cablerelease
release
416167-0000-000
416167-0000-000
Object guide 130x85 mm left
000000-1110-991
Axiovert200
200basic
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Axiovert
000000-1005-813
000000-1005-813
Binocular phototube
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000000-1005-828
Eyepiece E-PL 10x20 Br.
444231-9901-000
Eyepiece E-PL 10x20 Br. foc
4444232-9902-000
Eyepiece W-PL 10x23 Br. foc
000000-1016-758
Mounting frames for mechanical stage
Mounting frame K
451341-0000-000
Binocular tube 45°/23
000000-1005-827
Universal mounting frame K-X
451353-0000-000
Mechanical stage 130x85 R/L
without mounting frames
000000-1005-834
Mounting frame K, d=72mm
451344-0000-000
Stage stop d=28 mm
451345-0000-000
30
HighResolution
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MicroscopyCamera
CameraAxioCam
AxioCamb/w
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HighResolution
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MicroscopyColour
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000000-0412-312
Universal mounting frame K-Duo
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tube
Path
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000000-1069-227
Beampath
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P
Illuminator
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75
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Xenon
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lampXBO
XBO75
75W/2
W/2
Xenon
lamp
XBO
75
W/2
380053-9870-000
380053-9870-000
380053-9870-000
Protective
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gloves
Protective
gloves
417008-0000-000
417008-0000-000
417008-0000-000
Face
Faceprotection
protectionshield
shield
Face
protection
shield
417009-0000-000
417009-0000-000
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Reflector
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module
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Pol
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P&C
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FlFlP&C
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Analyzer
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P&C
P&C
000000-1050-958
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000000-1050-958
Baseport
Baseport60
60
000000-1005-824
000000-1005-824
AdapterVideo
VideoV200
V200CC2/3"
2/3"0.63x
0.63x
Adapter
frontport
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000000-1071-171
000000-1071-171
Universal mounting frame K
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1:1
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T2-adapterfor
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(Contax-Bayonet)
(Contax-Bayonet)
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416010-0000-000
TEM
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unitfor
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XBO75
75
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supply
unit
for
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XBO
75
90...250V,
90...250V,50...60Hz,
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130VA
VA
90...250V,
50...60Hz,
130
VA
000000-1003-924
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m
Cam
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A xi o
Ax
Specimen stage 250x230 mm
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Aria(D)
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SLR
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P P
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31
Lamp
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50
50with
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socket
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Lamp
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25
25
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50
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100...240V,
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50...60
50...60Hz,
Hz,
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350VA
350VA
000000-1113-833
000000-1113-833
000000-1113-833
32
AXIOBR_E.qxd
27.03.2001
14:14 Uhr
Seite 1
Microscopy from Carl Zeiss
System Overview
Axiovert
200 M
M
Axiovert 200
Axiovert 200
The New Standard
in Inverted Microscopy
Axiovert 200
G
er
m
an
y
2-axes control panel
457433-0000-000
only together
with LSM 510:
up
X
Y
Motor control MCU 28
457428-0000-000
MCU
PO
28
ER
WE
R
Dust cover
459330-0000-000
AxioCam and Light Trap are trademarks of Carl Zeiss.
HRZ 200 fine focusing stage
000000-1013-186
wn
RO
RU
34
R
N
56
78
CAN bus cable 2.5 m
457411-9011-000
Scanning stage DC 120x100
000000-1113-509
Path-deflecting mirror mot, Subbus
000000-1095-490
LD condenser 0.55 mot H, Ph 1, Ph 2, Ph 3, DIC, DIC
000000-1005-848
R
e
v
Excitation filter wheel
(8 positions) MOT
for 25 mm filters
000000-1008-106
98
Axiovert, AxioVision, LSM 5 PASCAL and Optovar is a registered trademark of Carl Zeiss.
R
do
Axiovert 200
Unique Down to the Last Detail
Live cell imaging, micromanipulation on living
cells – never before have such rigorous demands
been placed on the stability, flexibility, reliability
and operating comfort of microscopes. And
never before has there been a microscope capable of meeting these demands with such ease
and elegance. Its name: Axiovert 200, the state
of the art in inverted microscopy from Zeiss.
Axiovert 200 is based on the extraordinary
expertise and experience of a company which
has been setting international standards in
inverted microscopy since 1924. A company that
developed the first microscope with infinity
optics. But above all: this high-end microscope
surpasses earlier models in every crucial detail:
the outstanding Zeiss optics with the wide range
95
of ICS objectives, with innovative condensers and
415
642
Axiovert 200 M basic stand (*)
000000-1005-820
AxioVision Control Software
000000-1068-563
RS 233 cable
457411-9a012-000
REF
FO
CU
LEC
R
365
519
scope of sophisticated documentation and adaptation options, exceptional stability and unique
170
TO
S
297
Control PC-board with interface
000000-1015-381
(contained in basic stand)
E
ergonomic design.
187
O
RO
TIV
195
398
ZE
C
BJE
optimized fluorescence as well as with an unusual
Ax
io
Ca
m
And Axiovert 200 M gives you the freedom to
Adapter Video V200 C 2/3" 0.63x
at frontport
000000-1071-171
High Resolution Microscopy Camera AxioCam b/w
000000-0412-311
High Resolution Microscopy Colour Camera AxioCam Colour
000000-0412-312
468
150
590
294
Tube lens 1.0 for LSM
000000-1071-169
automate many routine procedures – so you save
time with no loss in reliablity.
265
As a matter of fact, Axiovert 200 is much more
Weight: Axiovert 200
ca. 24 kg (depending on model)
Axiovert 200 M ca. 40 kg incl. fluorescence
5-position mot. reflector turret, empty
000000-1064-365
5-position reflector turret, empty
000000-1064-364
than the sum of its benefits. And why? Because
it gives you a powerful basis to carry out your
work in all life science applications more precisely, more rapidly and more successfully than
For further details, please contact:
Beam path switching mot
000000-1069-229
33
LSM-adapter SP left and sideport 60 right, 3 switching positaions
000000-1071-164
LSM adapter BP and sideport 60 left, 3 switching positions
000000-1071-165
LSM adapter BP and FCS adapter SP left, 3 switching positions
000000-1071-166
Safety installations LSM 510/PASCAL
000000-1071-174
Beam path switching mot LSM
000000-1069-230
(*) If there are no special motorized components, all components of the manual stand
can also be used at the motorized stand.
Carl Zeiss
Light Microscopy
P.O.B. 40 41
37030 Göttingen
GERMANY
Phone : ++49 5 51 50 60 660
Telefax: ++49 5 51 50 60 464
E-Mail: [email protected]
www.zeiss.de/micro
Subject to change.
Printed on environment-friendly paper,
bleached without the use of chlorine.
before. In other words: it gives you the basis that
40-085 e/03.01
ensures you’re in the lead. And that’s exactly
where you need to be in science today.
If you need the best,
there is simply no alterantive
AXIOBR_E.qxd
27.03.2001
14:14 Uhr
Seite 1
Microscopy from Carl Zeiss
System Overview
Axiovert
200 M
M
Axiovert 200
Axiovert 200
The New Standard
in Inverted Microscopy
Axiovert 200
G
er
m
an
y
2-axes control panel
457433-0000-000
only together
with LSM 510:
up
X
Y
Motor control MCU 28
457428-0000-000
MCU
PO
28
ER
WE
R
Dust cover
459330-0000-000
AxioCam and Light Trap are trademarks of Carl Zeiss.
HRZ 200 fine focusing stage
000000-1013-186
wn
RO
RU
34
R
N
56
78
CAN bus cable 2.5 m
457411-9011-000
Scanning stage DC 120x100
000000-1113-509
Path-deflecting mirror mot, Subbus
000000-1095-490
LD condenser 0.55 mot H, Ph 1, Ph 2, Ph 3, DIC, DIC
000000-1005-848
R
e
v
Excitation filter wheel
(8 positions) MOT
for 25 mm filters
000000-1008-106
98
Axiovert, AxioVision, LSM 5 PASCAL and Optovar is a registered trademark of Carl Zeiss.
R
do
Axiovert 200
Unique Down to the Last Detail
Live cell imaging, micromanipulation on living
cells – never before have such rigorous demands
been placed on the stability, flexibility, reliability
and operating comfort of microscopes. And
never before has there been a microscope capable of meeting these demands with such ease
and elegance. Its name: Axiovert 200, the state
of the art in inverted microscopy from Zeiss.
Axiovert 200 is based on the extraordinary
expertise and experience of a company which
has been setting international standards in
inverted microscopy since 1924. A company that
developed the first microscope with infinity
optics. But above all: this high-end microscope
surpasses earlier models in every crucial detail:
the outstanding Zeiss optics with the wide range
95
of ICS objectives, with innovative condensers and
415
642
Axiovert 200 M basic stand (*)
000000-1005-820
AxioVision Control Software
000000-1068-563
RS 233 cable
457411-9a012-000
REF
FO
CU
LEC
R
365
519
scope of sophisticated documentation and adaptation options, exceptional stability and unique
170
TO
S
297
Control PC-board with interface
000000-1015-381
(contained in basic stand)
E
ergonomic design.
187
O
RO
TIV
195
398
ZE
C
BJE
optimized fluorescence as well as with an unusual
Ax
io
Ca
m
And Axiovert 200 M gives you the freedom to
Adapter Video V200 C 2/3" 0.63x
at frontport
000000-1071-171
High Resolution Microscopy Camera AxioCam b/w
000000-0412-311
High Resolution Microscopy Colour Camera AxioCam Colour
000000-0412-312
468
150
590
294
Tube lens 1.0 for LSM
000000-1071-169
automate many routine procedures – so you save
time with no loss in reliablity.
265
As a matter of fact, Axiovert 200 is much more
Weight: Axiovert 200
ca. 24 kg (depending on model)
Axiovert 200 M ca. 40 kg incl. fluorescence
5-position mot. reflector turret, empty
000000-1064-365
5-position reflector turret, empty
000000-1064-364
than the sum of its benefits. And why? Because
it gives you a powerful basis to carry out your
work in all life science applications more precisely, more rapidly and more successfully than
For further details, please contact:
Beam path switching mot
000000-1069-229
33
LSM-adapter SP left and sideport 60 right, 3 switching positaions
000000-1071-164
LSM adapter BP and sideport 60 left, 3 switching positions
000000-1071-165
LSM adapter BP and FCS adapter SP left, 3 switching positions
000000-1071-166
Safety installations LSM 510/PASCAL
000000-1071-174
Beam path switching mot LSM
000000-1069-230
(*) If there are no special motorized components, all components of the manual stand
can also be used at the motorized stand.
Carl Zeiss
Light Microscopy
P.O.B. 40 41
37030 Göttingen
GERMANY
Phone : ++49 5 51 50 60 660
Telefax: ++49 5 51 50 60 464
E-Mail: [email protected]
www.zeiss.de/micro
Subject to change.
Printed on environment-friendly paper,
bleached without the use of chlorine.
before. In other words: it gives you the basis that
40-085 e/03.01
ensures you’re in the lead. And that’s exactly
where you need to be in science today.
If you need the best,
there is simply no alterantive
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