Inverted Microscope OM900
Inverted Microscope
OM900
Instruction Manual
Please read the instructions carefully before operating
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CONTENTS
Safety
Parts List
Features
Assembly
Operation
Maintenance
Specifications
2
2
3
5
7
9
9
SAFETY
Disassembling the microscope may cause an electric shock or damage
to the instrument. Do not disassemble any parts of the microscope not
mentioned in the instruction manual. In case of a problem with the
microscope, contact Omâno.com, a professional repairman, or the
manufacturing factory.
Do not touch the bulb while microscope is in use. Bulb will become very
hot when in use. Allow bulb to cool before replacing. To prevent fire,
do not place combustibles near the bulb.
To avoid electric shock or damage to the instrument, unplug the
microscope before replacing the bulb. Use only the prescribed halogen
or fluorescent bulb. Wear gloves, or cover the bulb with a protective
sleeve. Do not touch the glass part of the bulb.
Turn off and unplug the microscope before moving.
Microscope should be kept at temperatures between
0°C-40°C/32°F-104°F, with a maximum humidity of 85%.
Do not place the microscope in direct sunlight or under direct indoor
light. This environment can influence the quality of the specimen
imaging.
Avoid dust. When not in use, cover the microscope with a dust cover.
Keep microscope on a level and sturdy surface.
Parts List
(1) 0M900 microscope
(3 each) Brightfield Objectives: 10x PL L,
25x PL L,40x PL L
(2) Bedplates
(1) Phase contrast objective: 10x PL L PHP
(1) Annular diaphragm slab
(3) Filters: blue, green, and frosted white
(2) Spare Bulb
(includes one 10x annulus ring)
(2) Eyepieces: 10X
(2) Eyepieces: 16X
(1) Instruction manual
(1) Dust cover
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Features
The Omano OM 900 Inverted Biological Microscope incorporates the best
optical technology, engineering and craftsmanship from around the world,
but at a price that is thousands of dollars less than comparable inverted
microscopes, elsewhere.
Inverted microscope design extends the benefits of the light microscope by
enabling you to observe micro-organisms in a large container under more
natural conditions. Large specimens such as tissue cultures, precipitates,
sediment and reactions are all well-suited for inverted microscopes. Since
the specimen is protected from the light heat, the sample life can be
extended while a container cover also helps reduce evaporation while still
allowing for gas exchange.
For example, you could take a sample of pond "scum" and observe it over
a period of several weeks. It would probably still sustains life although the
character of that life would probably change significantly over time from
when it was first collected. During this period, as the environment in the
petri dish changed, the life that it could support would change, but there still
would be a great deal of varied life.
By adapting your camera to the trinocular system you could photograph the
biological specimen you see or video tape progressive changes over time.
The Omano OM900 accommodates a variety of glass and plastic culture
vessels and flasks up to 2mm thickness and 100mm height.
Five possible levels of magnifications: 100x, 160x, 250x, 400x, and
640x
Eyepiece set included: wide field 10x (F.N. 18mm) and wide field
16X (F.N. 11mm)
Rotatable viewing head inclined 45°
Four plan achromatic long working distance DIN objectives
Brightfield objectives (Three) 10x/0.25 N.A. /8.1mm W.D., 25x/0.4
N.A./4.8mm W.D., 40x/0.6 N.A./3.3mm W.D.
Phase contrast objective (One) 10x/0.25 N.A. / 8.1mm W.D.
(Includes 10x Phase Annulus Ring)
Trinocular port increases functionality by adding photographic/video
capability
Centering telescope for phase contrast function
Above-stage long working distance (30mm) condenser, 0.4 N.A.,
with iris diaphragm, swing-out filter holder, and phase annulus ring
slot with ring centering knobs
Condenser centering system for precise positioning of condenser
over objective
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Rack & pinion steel gears for precision condenser movement
vertical to the stage
Oversized mechanical X-Y movable stage: 200mm x 152mm
Low position X-Y stage movement control - range of movement
78mm (X-Direction) x 36mm (Y-Direction)
Coaxial fine and coarse focusing knobs for left and right hand
Coarse adjustment range 20mm
Fine adjustment division 0.002mm
Tension adjustable coarse focusing
Variable controlled 6V/20W Halogen illumination with iris diaphragm
and light lens movement regulation
110 volt AC electrical supply
55-75mm inter-pupillary for distance adjustment between eyes
Diopter control for vision correction
ISO 9002 quality control standards
Durable metal alloys and reagent resistant finish
Includes three filters: blue, green, frosted white
Instrument Weight: 22+ LBS
Instrument dimensions: 17" high, 17" long, 8" wide
Molded Styrofoam shipping container for maximum protection
Limited Lifetime Warranty
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ASSEMBLY
1. Lamp box
9. Coarse focusing knob
2. Lamp box holding screw
10. Fine focusing knob
3. Filter Base
11. Objective
4. Annular diaphragm adjustment screw
12. Stage
5. Annular diaphragm slab
13. Condenser adjustment
6. Eyepieces
14. Aperture diaphragm swivel
7. Trinocular port
15. Lamp holder adjustment handle
8. Tensional adjustment ring
Figure 1
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Figure 2
16. Collector adjustment handle
22. Light control
17. Condenser holding screw
23. Limit knob
18. Condenser up/down knob
24. Tube holding screw
19. Lengthwise movement knob
25. Observation or photograph lever
20. Cross movement knob
26. Field diaphragm adjustment screw
21. Power switch
1.
2.
3.
4.
5.
6.
Refer to Figures 1 and 2 for assembly and operation
Remove from packaging and place on sturdy and level surface.
Install trinocular body on base and secure using thumbscrew (24).
Slide in eyepieces (6) into eyepiece tubes.
Lower the stage (12) by rotating the coarse focus adjustment (9) and remove
the shipping foam.
You can use either of the stage plates as shown in Figure 3. Match their use
to the specimen holder you are using.
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Fig. 3
OPERATION
1.
2.
3.
After assembly, plug the power cord in to an AC wall outlet and turn the power
switch (21) to the ‘1’ position.
Put a specimen on the center of the bedplate of the stage (12), and turn the
10x objective (11) into position (pointed directly at the bottom of the stage).
Adjust the light control knob (22) to obtain proper illumination. Note: It is not
necessary to have the illumination of the lamp to full brightness to obtain
proper image. Full brightness will shorten the life of the bulb.
1
Fig. 4
4. Adjust the interpupillary distance by rotating the prism covers to obtain a single
view through the eyepieces.(Fig. 4)
5. Adjust the position of the condenser light control (22) and the aperture
diaphragm swivel (14) to obtain satisfactory illumination.
6. Adjust the coarse (9) and fine (10) focus to obtain a sharp image from the
specimen.
Note: If one eye is not in focus, then focus using the right eye for a sharp image and
then adjust the diopter ring (Fig. 4 #1) to obtain sharpness in left eye.
7. If the lamp is in need of adjustment, using Figure 5, the vertical thumbscrew (1)
and the
Fig. 5
horizontal thumbscrew (2) are used to adjust the centering of the light. Removal of
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these thumbscrews allow for changing the bulb. Note: see the safety note about
handling the bulb.
8. Adjustment of the field diaphragm is accomplished while using the 10x
objective and after the specimen is seen clearly. Turn the field diaphragm
adjustment handle (26) to diminish field diaphragm, then turn the condenser
up/down knob (18) to gain the image of field diaphragm, then adjust condenser
adjustment screw (13) to center field diaphragm and eyepiece diaphragm, turn
field diaphragm adjustment handle to make field diaphragm less than eyepiece
diaphragm.
9. Adjustment of condenser aperture diaphragm is accomplished by turning the
aperture diaphragm swivel (14) to suit the numerical aperture of the condenser
with the numerical aperture of the objective, in this way, one can gain
satisfactory contrast and illumination.
10. Using the phase contrast.
Adjust the field diaphragm according to step 6, make the long working distance
condenser to move to corresponding position.
Insert the annular diaphragm slab (5) in the condenser, and turn the aperture
diaphragm swivel (14) to make aperture diaphragm maximum.
Turn the corresponding multiple phase contrast objective to the working
position.
Take out one eyepiece, and insert the center telescope in the eyepiece tube
and adjust it to make the image of the annular phase plane and annular
diaphragm clear. (See Fig. 6)
Center Telescope
Fig. 6
Fig. 7
Adjust the annular diaphragm adjustment screw (4) to make annular
diaphragm light halo and annular phase plane dark halo superimposed as
shown in Figure 7 on the right circle.
Take out the center telescope and insert the eyepiece to observe phase
contrast. Note: It may be necessary to readjust the annular diaphragm when
observing with phase contrast.
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MAINTENANCE
1. When replacement of the lamp is necessary, be sure to turn off power and
remove plug. Loosen thumbscrews (1 and 2 in Fig. 5) and remove lamp
holder (3) from the lamp box (1). Pull out old bulb. Handle new bulb with
gloves or tissue to keep from touching bulb. Oil from the skin left on the bulb
will shorten bulb life. Install new bulb into socket. Check illumination and
check for proper alignment of bulb (See Operation #7).
2. If fuse replacement is necessary, loosen screw top of fuse. Pull out the fuse,
replace, and retighten fuse cap.
3. If dirt has settled on the lens, wipe it off gently with some lens paper. If
alcohol is used, be careful not to let it penetrate through the lenses and
dissolve the epoxy. Any dust settled on the lenses can be blown away with
an air blower or wiped off with a clean soft camel hairbrush or similar.
2. In cleaning mechanical parts and applying non-corrosive lubricant, take special
care not to touch the optical elements, especially the objective lenses.
3. Avoid high temperatures.
4. Put objectives and eyepieces into box after using, and keep them in a cool, shady
and dry place, free from dust, fumes and moisture. If not stored in its box, cover with
a hood to protect from dust.
5. Like other optical instruments, the microscope should be kept out of direct sunlight
in a cool, dry place, free from dust, fumes and moisture. It should be stored in a
case or covered with a hood to protect it from dust.
6. The microscope has been carefully tested and inspected. Please do not
disassemble, but refer to a qualified technician.
Specifications
1. Eyepiece
Type
Focus
Vision field’s
(mm)
diameter (mm)
Magnification
Wide field eyepiece
10X
25
18
Plan eyepiece
16X
15.6
11
2. Objective
Numerical
W.D.
Aperture (N.A.)
(mm)
10X
0.25
8.1
---
25X
0.40
4.8
0.12
40X
0.65
3.3
0.12
10X P.C.
0.25
8.1
---
Magnification
Thickness of object stage (mm)
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3. Total magnifying powers
Objectives
Eyepieces
10x
25x
40x
10x
100x
250x
400x
16x
160x
400x
640x
Total Magnification
4. 6V20W illumination and adjustable brightness
5. Power is 110V AC
6. Binocular head
7. Attachable mechanical stage: size is 200mm X 152mm, moving range is 30×70
8. Long Working Distance Condenser (with phase contrast): N.A=0.4, working
distance: 28mm
9. Interpupillary distance: 53~75mm
10. Minimum division of fine focusing: 0.002mm
NOTES:
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