INCUBATOR CO2Cell 190 Comfort CO2Cell 190S Comfort

INCUBATOR CO2Cell 190 Comfort CO2Cell 190S Comfort
Instructions for use
CO2 INCUBATOR
CO2Cell 190 Comfort
CO2Cell 190S Comfort
(with sterilization 200 °C)
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
CONTENTS:
24. 1. 2013
1
SAFETY .......................................................................................................................................................3
1.1
1.2
1.3
1.4
2
Legal considerations............................................................................................................................. 3
Intended use. ........................................................................................................................................3
Symbols used in this manual.................................................................................................................. 3
General safety instructions. ................................................................................................................ 3
DESCRIPTION..............................................................................................................................................3
2.1
Construction........................................................................................................................................4
2.2
Doors...................................................................................................................................................4
2.3
Control system. ...................................................................................................................................4
2.4
Co2 sensor. .......................................................................................................................................... 4
2.5
Diagrams...............................................................................................................................................4
2.5.1 Outer view . ..................................................................................................................................... 4
2.5.2Inner view ........................................................................................................................................ 4
2.5.3 Rear connections ............................................................................................................................ 4
3
DELIVERY, TRANSPORTATION AND STORAGE....................................................................................... 5
3.1
3.2
3.3
4
INSTALLATION.............................................................................................................................................5
4.1
4.2
4.3
4.4
4.5
5
Location and ambient conditions............................................................................................................ 5
Shelves and usable space...................................................................................................................... 6
Water tray. .......................................................................................................................................... 6
Gas connection.....................................................................................................................................6 Electrical connection........................................................................................................................... 6
GETTING STARTED.....................................................................................................................................7
5.1
5.2
5.3
5.4
5.5
5.6
6
Unpacking. ............................................................................................................................................5
Lifting and moving................................................................................................................................. 5
Storage................................................................................................................................................5
Start up ...............................................................................................................................................7
Setting time and date. ........................................................................................................................... 7
Factory presets. .................................................................................................................................. 7
Changing temperature set points and high / low alarms......................................................................... 7
Changing co2 set point and alarm thresholds........................................................................................ 8
Alarm status screen. ............................................................................................................................ 8
OTHER FEATURES...................................................................................................................................... 9
6.1
Graphical display.................................................................................................................................. 9
6.2
Utility menu. .........................................................................................................................................9
6.3
Door alarm menu. ...............................................................................................................................10
6.4
Incubator information. ........................................................................................................................ 10
6.5
Historical event log. .......................................................................................................................... 10
6.6
User security. ....................................................................................................................................10
6.6.1 Entering a user name and password................................................................................................ 10
6.6.2 Enabling security. .......................................................................................................................... 10
6.6.3 Logging out.....................................................................................................................................11
6.6.4 Disabling security............................................................................................................................11
7
SD CARD DATA LOGGING........................................................................................................................ 11
7.1
7.2
7.3
8
Start logging. .................................................................................................................................... 11
Changing filename............................................................................................................................... 12
Removing the sd card.......................................................................................................................... 12
HIGH TEMPERATURE STERILISATION................................................................................................... 13
8.1
8.2
8.3
8.4
8.5
Preparation. ....................................................................................................................................... 13
Starting the cycle.............................................................................................................................. 13
Sterilise cycle....................................................................................................................................13
Cooling phase.....................................................................................................................................14
Aborting the sterilise cycle................................................................................................................ 14
9
ERROR MESSAGE SCREENS.................................................................................................................. 14
10
OPTIONS ...................................................................................................................................................15
10.1
10.2
10.3
10.4
10.5
Volt free alarm relay (bms)................................................................................................................. 15
4-20 ma retransmission (retx)............................................................................................................... 15
Rs 232 serial communication (232). ....................................................................................................... 15
Ethernet communication (eth).............................................................................................................. 15
Multi-door (8id)...................................................................................................................................15
11
ACCESSORIES..........................................................................................................................................15
12
CLEANING..................................................................................................................................................16
12.1
12.2
12.3
13
Outer surfaces...................................................................................................................................16
Inner surfaces....................................................................................................................................16
Oxidation at high temperature (comfort 190s only). ............................................................................. 16
SERVICE.....................................................................................................................................................16
13.1
13.2
13.3
Warranty. ..........................................................................................................................................16
Preventative maintenance.................................................................................................................... 16
Returning a unit..................................................................................................................................16
14
DISPOSAL..................................................................................................................................................17
15
TECHNICAL INFORMATION......................................................................................................................17
15.1
15.2
Technical data..................................................................................................................................... 17
Dimensional drawings.......................................................................................................................... 17
Instructions for use
1 safety
These safety instructions are intended to be used alongside
any local safety guidelines, rules and regulations. If in
doubt please consult the Health and Safety Officer of your
organisation.
1.1 legal considerations
It is important that you read this manual
completely and observe all instructions.
Failure to follow these instructions may result
in personal injury, equipment damage and
incorrect performance of the incubator.
This manual contains information for installing, operation
and maintenance of the incubator. It cannot cover all
possible uses and applications. Please contact your dealer
or MMM directly if you have a special application or the
manual does not describe your problem in enough detail.
The contents of this manual and the incubator are subject to
change without notice.
Only skilled electricians or engineers authorised by MMM
can perform repairs or maintenance on this incubator. Only
original spare parts from MMM can be used. There are no
user serviceable parts within the incubator.
MMM takes no responsibility for 3rd party accessories or
equipment used with this incubator.
Electrical hazard
Danger of death
DANGER
Danger of burning
Risk of injury
1.2 intended use
The CO2 Cell Comfort 190 incubators are intended for
mammalian cell cultivation under typical conditions of
37.0 °C. The pH of the cell media can be controlled by
altering the CO2 concentration within the incubator.
The glass door and inner chamber reach
200 °C during sterilisation.
HOT
SURFACES
The following symbols are used throughout this manual:
Risk of injury Electrical
hazard
Stability
hazard
Read
operating
manual
Compressed
gas hazard
Lift with
several
persons
Hot surface
Lifting
hazard
Asphyxiation Mandatory
hazard
regulation
Disconnect
from
electrical
supply
Important
information
for correct
operation
1.4 general safety instructions
This incubator is intended for use by suitably qualified
laboratory personnel who are familiar with all precautions
and standard safety practices for working in a laboratory
environment.
Do not force the door open during
sterilisation.
Stability hazard
Risk of injury
Damage to unit
Other applications are not approved by MMM. Please
contact your dealer or MMM directly, for advice if you intend
to use this incubator for other applications.
1.3 symbols used in this manual
Do not remove any panels from incubator.
Do not allow the incubator to become wet.
STABILITY
HAZARD
Do not place the unit too close to the edge
of a bench.
Do not lean on the door when open.
Lifting hazard
Risk of injury
Damage to unit
LIFTING
HAZARD
Lift with 4 people
95 kg
Observe safe handling regulations
2 description
CO2 Cell Comfort incubators are fitted with the latest
generation of programmable controller, ensuring precise
control of temperature and CO2 levels.
Accurate and stable heating throughout the entire chamber
is ensured by the unique MMM designed five sided direct
heating element. In addition a separate independently
controlled door heater is fitted to ensure that the inner glass
door is free from condensation.
The design of these element profiles promote natural
convection of the air within the chamber, ensuring even
temperature and gas distribution throughout the chamber.
CO2 Cell Comfort incubator has extra heaters that allow
high temperature 200 °C dry sterilisation of the incubator
chamber.
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
3
Instructions for use
2.1 construction
The inner chamber is made from 316 grade stainless steel.
It is deep drawn from one piece with no welds and is
electro-polished, meaning that the inner surfaces are
smooth and easily cleaned.
The sensor is not mounted within the chamber; instead a
small pump passes the chamber atmosphere through the
sensor.
The sensor does not have to be removed during high
temperature sterilisation.
The removable shelves, racking and water tray are also
made from stainless steel.
2.5 diagrams
The outer housing is made from stainless steel and powder
coated with anti-bacterial biocide paint.
2.5.1outer view
2.2 doors
The outer door has a heater on its inner face. This door is
an integral part of the temperature control system and must
be closed for the incubator to function normally.
The door is hinged on the right hand side. Left hand opening
is only available as an option at the point of ordering, retrofitting is not possible.
When this door is open, the CO2 valve is closed and
heater power is reduced to prevent over shoot of operating
conditions. The door is linked to the controller alarm system.
aSection 6.3
On the Comfort 190S incubator, 2 magnetic solenoid
locks prevent the outer door from being opened while the
incubator is in a high temperature sterilise cycle.
An inner glass door seals against a thermoplastic elastomer
gasket. This allows viewing of the samples within the
chamber without disturbing the temperature or atmosphere
of the chamber.
2.5.2inner view
The incubator can be optionally equipped with a multi-door.
These multi-doors improve temperature and CO2 recovery
after a door opening. Multi-doors can be retro fitted.
aSection 10.
2.3 control system
The colour LCD touch screen display allows the user to
easily see operating conditions and to alter settings through
icon based menus.
Software controlled PID loops constantly monitor
temperature and CO2 values, making automatic adjustments
to keep the chamber atmosphere within limits.
The controller is equipped with a built in feature that logs
incubator data directly to a removable SD card. This
data is in Excel format, making it easy to view with a PC.
aSection 7.
A RS 232 serial port is fitted as standard. This allows you
to connect the incubator to a PC so that you can remotely
access the SD card data. PC software is required to do this.
Contact your dealer or MMM directly for advice.
Other optional communication options are Ethernet PC
interface and 4-20 mA retransmit. aSection 10.
2.4 co2 sensor
CO2 is measured and controlled using a highly accurate
infra-red sensor. This sensor remains practically drift free
between calibration periods.
4
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
2.5.3rear connections
Instructions for use
3 delivery,
3.3 storage
3.1 unpacking
Move the incubator to its final location and wait for 4 hours
before start up. This will allow any condensation that may
have formed to dry. It will also stop the incubator from
overshooting temperature set point.
transportation
and storage
If the incubator has been in a cold location for a
period then it needs to acclimatise to room
temperature.
Inspect the packaging for signs of transportation damage.
Carefully undo the top of the cardboard packaging and
gently slide the packaging over the incubator.
Inspect the incubator and inform the carrier immediately if
there are any signs of transportation damage.
Remove any packaging material from within the chamber.
Remove any protective coverings from the surfaces of the
inner chamber.
Remove the operating manual and accessory kit from within
the chamber. Keep these safe.
Please check that the accessory pack contains the following
items. Contact your dealer with details of any shortages.
Comfort 190 accessory pack
Qty
Stainless steel perforated shelves
4
Shelf racking (left and right hand)
1 set
Racking support bar
1
Stainless steel water tray
1
Service port bungs
2
Formatted 2Gb SD card
1
Instructions for use
1
3.5 meter length of CO2 tubing with HEPA
filter
1
Hose clips
2
Test report
1
IEC mains power lead
1
3.2 lifting and moving
These incubators are heavy. The weights are as follows:
Comfort 190 is 94,5 Kg
Comfort 190S is 104 Kg
Please refer to Manual Handling Policy of your organisation
before attempting to lift the incubators.
Risk of unit sliding or tipping.
Risk of injury by lifting heavy loads.
Risk of damage to the unit.
Always lift using the handles on the outside
of the incubator.
Lift with 4 people.
Do not lift by using the door.
4 installation
4.1 location and ambient
conditions
The incubator can be placed on a table or worktop.
Please ensure that the site is sufficiently strong
enough to carry the weight of the incubator.
MMM do not recommend placing the incubator directly on
the floor as this increases the risk of contamination.
Wheeled platform forms are available that lift the incubator
off the floor. Alternatively two incubators can be stacked
using the stacking kit. aSection 10.
Correct location of an incubator is very important. The
incubator is designed to work in a typical laboratory
environment.
For good performance the ambient conditions should be at
least 6 °C below the working temperature.
E.g. for a set point of 37.0 °C the ambient temperature must
not exceed 31.0 °C.
If the difference between the set point and ambient
temperatures is less than 6.0 °C then the set point can be
exceeded and temperature fluctuation will occur.
Our performance data was produced at the ideal ambient
condition of 25.0 °C. For best performance the ambient
temperature should not drastically exceed this figure.
Maximum ambient humidity is 70 % rH, non-condensing.
Condensation can occur within the chamber if this figure is
exceeded.
A poorly located incubator can experience temperature
fluctuations, poor cell growth and contamination of cell
cultures. These guidelines will ensure best performance:
• The incubator should be sited on a flat, even surface.
• It should be in a well ventilated and dry area, but not
sited directly in the path of ventilation ducts.
• It should be free from vibration.
• It should be kept away from heat sources e.g. radiators,
heating pipes, refrigeration plant.
• It should not be in direct sunlight.
• To avoid contamination it should not be placed directly
on the floor.
• Allow sufficient distances between adjacent equipment.
• There should not be big swings in ambient temperature.
Leave a clearance of 50 mm from each side and 100 m from
the rear.
Level the incubator by adjusting the two front feet. Place a
small spirit level on the centre position of the middle shelf as
a guide.
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
5
Instructions for use
When it is level, secure the feet by tightening the lock nuts
with a 10 mm spanner.
4.2 shelves and usable space
Each incubator is supplied with 4 perforated stainless steel
shelves. The shelves locate into removable stainless steel
racking. The shelves incorporate an anti-tilt mechanism,
stopping the shelves from being pulled out too far.
Each shelf measures 690 mm wide by 390 mm deep. The
minimum spacing between shelves is 80 mm. There are
8 possible positions for the shelves.
The shelves and racking can be left in place during sterilisation.
The usable space within the incubator is the volume
in which the conditions are within our published
data.
4.4 gas connection
Risk of injury. Compressed gas.
Risk of damage to unit.
Observe relevant regulations for
handling CO2
Risk of asphyxiation. Site must have
adequate ventilation.
Risk of CO2 poisoning. Site must have
adequate ventilation.
The incubator is designed for use with CO2 of
99.5 % purity. Input pressure greater than 1.0 bar
(15 psi) will damage the incubator so a pressure
reducing valve (PRV) must be used.
The CO2 inlet to the incubator is via a 4 mm barbed push
fitting.
PRV’s with the correct fittings and other gas accessories
can be supplied by MMM. aSection 10.
For correct connection of CO2, follow these steps in order:
A, B, C = Internal dimensions
a = distance from left and right wall = 70 mm
b = distance from top = 70 mm
c = distance from front and back wall = 70 mm
d = distance from base = 70 mm
For practical purposes this is the perforated area on each
shelf.
• Do not place any samples outside of this space.
• Do not over fill each shelf.
• Allow sufficient room between samples to allow for
airflow.
• Do not use the top shelf for very deep samples.
4.3 water tray
A stainless steel water tray is supplied which allows the
incubator to provide 90 %rH.
Place the tray is on the floor of the incubator, locating it with
the side racking.
Carefully fill the tray with 1.5 litres litres of distilled, sterilised
water.
MMM recommend that you clean and refill the tray every
week.
Any spillages within the chamber should be removed and
cleaned immediately. Water left on the chamber floor will
cause condensation to form.
Under no circumstances should any equipment be placed in
the water tray or on the chamber floor.
Remove the water tray before moving the incubator.
Empty the water tray if the incubator is switched off,
otherwise condensation will occur.
6
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
1) Connect the supplied tubing and HEPA filter to the gas
supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between
0.3 bar and 0.7 bar (4.5 to 10 psi).
5) Close the main valve on the gas bottle / central supply.
6) Connect the other end of the tubing to the incubator CO2
inlet. Secure with the supplied clip.
Repeat these steps whenever you change the CO2 bottle or
supply.
Turn off the main valve and disconnect from the CO2 supply
if the incubator is to be unused for extended periods.
4.5 electrical connection
Risk of injury.
Risk of damage to unit. Observe correct
mains supply voltage.
Risk of electrical shock. Do not connect
with wet hands.
No user serviceable parts. Do not
remove service panels.
Disconnect unit in an emergency.
Before connecting the incubator, check that the electrical
supply corresponds with the values on the incubator data
plate. If in doubt consult a qualified electrician.
The incubator is designed to operate from a single phase
electrical supply of: 230 V AC ±10 % 50/60 Hz.
This incubator MUST be connected to a protective earthed
supply.
Further details can be found in the technical data.
aSection 15.1.
Instructions for use
Plug the supplied power lead into the IEC connector on the
rear of the incubator.
Plug the other end of the IEC lead into the electrical supply.
In an emergency you may need to isolate
the incubator from the electrical supply by
disconnecting the mains supply plug. Make sure
that the mains supply plug is easily accessible.
5 getting
started
5.1 start up
main display
Door open alarm timer
seconds
The
main screen30 shows
chamber set points (SP) and
30 seconds
process
values (PV). It also shows the status of any
Main display
alarm
and has touch screen buttons for accessing further
The main
screen shows chamber set points (SP) and process values (PV). It also shows the status of any
Main
display
alarm and has touch screen buttons for accessing further functions.
functions.
Door open alarm timer
The main screen shows chamber set points (SP) and process values (PV). It also shows the statu
alarm and has touch screen buttons for accessing further functions.
Temperatureprocess value
Chamber temperature set point
CO2 process value
Chamber CO2 set point
Sterilise cycle start
Menu escape
Temperatureprocess value
Insert the pre-formatted SD card (supplied) into the slot to
the right of the controller.
Chamber temperature s
CO2 process value
Chamber CO2 se
Turn on the unit using the power switch on the rear panel.
The controller will boot up and perform a self-check.
5.2 setting time and date
After the controller has booted up, the time and date screen
will be shown. Time and date are shown in the format
hh:mm:ss and dd/mm/yy.
5.4.
Changing temperature set points and high / low alarms.
5.4 changing temperature set
points and high / low alarms
Sterilise cycle start
Menu escape
To change the temperature set point, touch this area of the main screen:
To change the temperature set point, touch this area of the
5.4.
Changing temperature set points and high / low alarms.
To change the temperature set point, touch this area of the main screen:
Touch here to alter set point
Touch here to alter high alarm limit
main screen:
Touch here to alter low alarm limit
If the time and date is correct
press:
Use the key pad to change the set point.
Touch
here to
to alter
setset
pointpoint
Touch
here
alter
Maximum set point is limited to 60.0 °C
If the time and date are incorrect press the time display. This
screen will be shown:
Press
Jan 2012
Comfort 190 & 190s Iss.2
Use the keypad to
5.3 factory presets
The following values are preset to allow you to work straight
away:
Parameter
Set Point, Temperature
Set Point, CO2
High temperature alarm
Low temperature alarm
High CO2 alarm
Low CO2 alarm
Door open alarm timer
change
the
settopoint.
Maximum
set point is
limited
60.0 °C
set point
MinimumMaximum
set point is limited
to 25 °Cis
Press
to confirm
Repeat these steps for setting
the date.
Value
37.0 °C
5.0 %
+1.0 °C
relative to set point
-1.0 °C
relative to set point
+1.0 °C
relative to set point
-1.0 °C
relative to set point
30 seconds
Touch here to alterPage
low22 of 48
alarm limit
Use the key pad to change the set point.
Use the touch screen to enter
the correct time.
Press
selection.
high
alarm limit
Touch here to alter
high
to confirm
selection.
alarm
limit
Touch here to alter low alarm limit
here
Minimum set pointTouch
is limited
to 25to
°Calter
Proceed to step 5.3
Jan 2012
Comfort 190 & 190s Iss.2
limited to 60.0 °C
to confirm selection.
Minimum set point is
limited to 25 °C
Press
selection.
Page 22 o
to confirm
The high and low alarm limits are relative to the chamber set
point.
For instance if the set point is 37.0 °C and the high alarm
limit is + 1.0 °C then the alarm will trigger at 38.0 °C.
If the set point is changed to 36.5 °C then the high alarm will
trigger at 37.5 °C.
Use the keypad to
change the high alarm
threshold.
Press
selection.
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
to confirm
7
Instructions for use
Use the keypad to
change the low alarm
threshold.
This must be negative
so remember to first
Use the keypad to
change the low alarm
threshold.
This must be negative
so remember to first
press
press
Press
selection.
Press
selection.
to confirm
to confirm
5.5 changing co2 set points and
5.6 alarm status screen
To change the temperature set point, touch this area of the
The alarm icon
status.
main screen:
Pressing the alarm icon opens the alarm status screen:
alarm thresholds
gives a visual indication of alarm
Touch here to alter set
point
Touch here to alter
high alarm limit
Touch here to alter low
alarm limit
Use the keypad to
change the set point.
High temperature alarm
Low CO2 alarm
Maximum set point is
limited to 20.0 %
Low temperature alarm
Door open alarm
High CO2 alarm
SD card alarm
Minimum set point is
limited to 0.0 %
Press
to
confirm selection.
Low gas pressure alarm
In an alarm condition an audible buzzer sounds and the
If the CO2 set point is 0.0 % then CO2 control is
disabled.
Use the keypad to
change the high alarm
threshold.
Press
selection.
to confirm
alarm icon changes to red.
Press this red alarm icon and the alarm status screen will
show the corresponding alarm will in red.
For instance this shows high temperature alarm condition:
Pressing this will mute the alarm.
The alarm is now acknowledged and the icons show this by
changing to yellow:
The icons remain yellow for as long as the incubator is in an
alarm condition.
This process is the same for all alarms.
8
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
Instructions for use
As an additional warning the colours of the process values
change during alarm condition.
The temperature process
value has changed
colour from black to red.
The CO2 process value
will change colour from
blue to red when it
enters alarm conditions.
6 other
The CO2 graph is viewed in the same manner.
6.2 utility menu
On the main screen press the utility menu symbol:
The utility menu combines all of the incubator settings
menus in one place. You will already be familiar with some
of these.
features
6.1 graphical display
6. Other features
The controller has a built in feature that allows the user
to view graphs of both temperature and CO2. It stores a
6.1. Graphical display
minimum of 3 days of graphical data. When the internal
The controller hasmemory
a built in feature
thatitallows
the user to view
graphs
of both
temperature
is full
will over-write
the
oldest
data
first. and CO2.It
Clock menu. aSection 5.2
Temperature menu. aSection 5.5
stores a minimum of 3 days of graphical data. When the internal memory is full it will over-write the oldest
Graphs are only stored while the unit is powered.
data first.
This feature is independent of the SD data logging function.
Graphs are only stored while the unit is powered.
aSection 7.
This feature is independent of the SD data logging function.  Section 7
CO2 menu. aSection 5.6
On the main screen press the graph symbol:
User security menu. aSection 6.6
SD card menu. aSection 7
Door alarm menu. aSection 6.3
Alarm status menu. aSection 5.7
Historical event log. aSection 6.5
Incubator information. aSection 6.4
User log out. aSection 6.6
On the main screen press the graph symbol:
The graph menu
opens:
The graph menu opens:
Choose which graph
you
view
Choose which graph
youwish
wish toto
view
In this instance we are viewing the temperature graph:
In this instance we are viewing the temperature graph:
Press the y-axis limits.
Press the
y-axis limits.
The values can be changed using the keypad.
The values
can
to view
thebe
temperature stability against time.
changed
using
the
To return to the Main Screen press the escape button
keypad.
Press
Jan 2012
Use
Comfort 190 & 190s Iss.2
Press
Use
stability against time.
Page 26 of 48
to view the temperature
To return to the Main Screen press the escape button
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
9
Instructions for use
6.3 door alarm menu
Press the door icon:
The door has a
magnetic switch that
senses when the door
is opened.
6.6 user security
The door icon turns red as soon as the door is opened. After
a set time the alarm will sound. This can be muted in the
alarm status menu. aSection 5.7
This incubator allows for a user name and password
to be programmed.
6.5. User security
This delay can be changed by pressing here.
The format is mm:ss.
This stops any unauthorised changes to incubator settings.
This incubator allows for a user name and password to be programmed.
6.6.1
a user
name
and
6.5. User security
This
stops anyentering
unauthorised
changes
to incubator
settings.
6.4 incubator information
password
This incubator allows for a user name and password to be progra
To enter
user name
andname
password
press the User icon:
6.5.1.a Entering
a user
and password
Press the info icon:
This stops any unauthorised changes to incubator settings.
The information screen shows the incubator Serial Number
as well at what release of software / firmware the controller
is using. This information is important should you require
any assistance from MMM.
To enter a user name and password press the User icon:
6.5.1. Entering a user name and password
To enter a user name and password press the User icon:
Press here on Line 1 to
enter user name.
Press here on Line 1 to enter user nam
Press:
to save.
Press:
save.
to
Then press here to enter password.
here
on Line 1
Then press
Press: here to Press to
save
Press:
enter password.
6.5.2. Enabling security
Press:The user name and password are now
Then press here to e
to save
The user name and password are now saved.
Press the security status icon:
6.6.2enabling security
6.5 historical event log
The controller has a built in feature that displays the time
and date of 100 historical events.
Press:
The icon changes state to:
The user name and p
6.5.2. Enabling security
Security
is enabled
and you
are
Pressfeature
the security
status
icon:
now logged on as shown by the open padlock sym
Press the security status icon:
The icon changes st
When 100 events have been logged the oldest event is
over-written.
The icon changes state to:
Events include: alarms, alarm acknowledgment, door open/
closed, power on/off, SD card insertion/removal,
user logon/logoff, sterilise cycle start/finish/abort.
Security is
feature
is enabled
and
you
are logged
now logged on as shown by the o
Security feature
enabled
and you
are
now
Press the event log icon:
though the events.
10
Press
on as shown by the open padlock symbol:
to scroll
Jan 2012
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
Comfort 190 & 190s Iss.2
P
Instructions for use
6.6.3logging out
7.1 start logging
On the utility menu screen, press and hold the log out icon:
If the card is already inserted when the incubator is powered
up then logging will start automatically.
You are logged out and the padlock icon is now locked:
No changes to the incubator can be made without logging
back in.
For the Comfort 190S incubator only:
At this point the magnetic door locks are activated, stopping
any unauthorised access to the incubator.
6.6.4disabling security
If the SD symbol is grey
then the incubator is
logging. If it is red then it is not logging.
Press the red SD icon
in the Utility Menu. The Status
screen will state that the card is removed.
Press the utility icon:
When prompted, enter the user name and password.
To disable security press:
Icon changes to:
Security is now disabled.
7 sd
card data
logging
The incubator is supplied with a 2 GB SD card. This
SD card is pre-formatted to work with the
controller’s operating system.
Insert the SD card into the slot to the right of the controller
(label side closest to controller).
The SD Card status screen shows that the controller is
locating the data and preparing to log. This can take from a
few seconds to a few minutes depending on data file size.
Please be patient.
Only cards supplied by MMM can be used. Using
any other type of card will not work with the
incubator and will cause the controller system to
crash.
The controller can log to a removable SD card located on
the front of the incubator.
Every 30 seconds the controller records the following data
to the SD card:
Date & Time
Chamber temperature set point (SP);
Chamber temperature process value (PV)
Door set point (SP);
Door process value (PV)
Door set point (SP); Door process value (PV)
CO2 set point (SP);
CO2 process value (PV)
Any events are also logged in a separate file.
When the controller has located the log file the status screen
shows that it is logging data:
Press
to return
to the main screen
The data is stored in Excel format making it very easy to
read and use the data with a PC.
Data is only logged when the incubator is powered; however
data is not lost when power is removed.
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
11
Instructions for use
7.3. Removing the SD card
7.2 changing filename
You can easily change the filename to start a new log.
Always perform the following steps before
7.3 removing
the sd card
these steps will results in corrupted data.
Always perform the following steps before
removing the SD Card. Failure to follow
these steps will results in corrupted data.
Press the filename on the screen, in this case 12345. Enter
a new file name, in this case 67890.
Press
to store selection.
On
SDSD
Card
statusstatus
screen screen
press thepress
SD cardthe
tools
icon:
Onthe
the
Card
SD
card
tools ico
Press the remove card
icon:
Press
Press the rem
to confirm the new filename:
Press the confirm
icon:
Press the con
The controller will then locate the new file and start logging:
It is now safe to
remove the SD card.
It is now safe
When the card is removed then the screen will automatically
change to this:
When the card is removed then the screen will automatica
12
Jan 2012
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
Comfort 190 & 190s Iss.2
Instructions for use
8 high
temperature
sterilisation
This shows that the door locks have activated and that
Operator 6 is logged on.
Risk of damage to unit.
The Comfort 190 is not intended to be used as
an autoclave and must not be used for sterilising
any other equipment.
Press
proceed.
to
Only the shelves, racking and water tray can be
left inside the incubator during sterilisation.
Risk of injury. Risk of burning. Internal surfaces
reach 200 °C
The Comfort 190S incubator is equipped with a high
temperature sterilisation function. This raises the chamber
temperature to 200 °C for 2 hours.
Independent evaluation has shown this to be effective
against all spores and bacteria commonly associated with
laboratory incubator contamination.
8.3 sterilise cycle
The sterilise cycle has now started.
A temperature graph is plotted to show
the duration of the cycle. The y-axis
automatically changes its scale to 205 °C
8.1 preparation
This shows that
the heaters are
operational
Make sure that you have entered a user name and
password as shown in aSection 6.6.1
Empty and dry the water tray. This can be placed back
inside the incubator.
Abort cycle icon
aSection 8.5
Clear any samples or equipment from the shelves.
The shelves and racking can be left in place during the
sterilisation cycle.
8.2 starting the cycle
The sterilise cycle consists of 3 distinct phases. They are:
Press and hold the sterilise button for 5 seconds:
During the heat up phase, maximum power is applied to
the heating elements. CO2 control is disabled to protect the
sensor and the door locks are activated.
• Heat up.
The first sterilise cycle check list opens:
• 2 hour dwell.
Automatically carried
out by controller.
The dwell phase starts once the chamber temperature
reaches 200 °C.
Heater power is automatically reduced to regulate the
temperature at 200 °C.
• Cool down.
When you have emptied the water tray, place it back inside
the chamber and close the doors.
Press the grey tick to acknowledge this:
All checks are now
complete.
Press
proceed.
to
After 2 hours at 200 °C power is removed from the heaters
and the cooling phase begins. The chamber is allowed to
cool naturally. When the chamber temperature reaches
38 °C the door locks are deactivated and CO2 control is
enabled.
The time taken for a full sterilisation cycle is
dependent on the cool down phase.
As cooling relies on the heat loss through the chamber walls
the time taken is affected by the location of the incubator
and the ambient temperature.
So, an incubator placed under a worktop will take longer to
cool than a freestanding incubator on a worktop.
We recommend that you carry out the sterilise cycle at the
end of a working day. That way, when you return to work in
the morning the incubator will be ready for you to use.
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
13
Instructions for use
This sterilise cycle graph shows a typical start to finish
duration of 8 hours:
8.5 aborting the sterilise cycle
Should you need to stop the sterilise cycle for any reason
then press the abort icon:
8.4 cooling phase
After the 2 hour dwell is completed the incubator goes into
the cooling phase of the sterilisation cycle.
The doors are locked during this phase. CO2 control is
disabled.
The chamber
temperature is
displayed
Press
The incubator now enters the cooling phase.
aSection 8.4.
The cooling phase ends once the temperature falls below
38.0 °C
9 error
message
screens
In the event of a fault condition the control system may warn
you with various error messages.
Chamber probe failure
Press
to continue
to main screen.
Chamber probe is out of
limits and has failed.
Contact MMM Service
immediately
Base probe failure
Base probe is out of limits
and has failed.
Contact MMM Service
immediately
Door probe failure
Door probe is out of limits
and has failed.
Contact MMM Service
immediately
14
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
Instructions for use
SD card critical error
SD has not been safely
removed.
The incubator will be supplied with a pre-wired 3 metre
cable socket.
Press and hold
Switch
power off, insert card and
switch power on again.
If problem persists contact
MMM Service
SD card removal error
Repeat the steps above to
fix this.
Boot screen error
Take a picture of the
screen. Turn power off for
1 minute.
If problem persists contact
MMM Service with details
of picture.
10options
10.1 volt free alarm relay (bms)
All touch screen incubators are fitted as standard with a
volt free changeover relay for connection to a building
maintenance system (BMS).
This relay changes state whenever the incubator is in an
alarm condition. The relay changes back to its original state
once the alarm condition clears.
The connections are as shown below:
The temperature signal is scaled as: 4 mA = 0 °C;
20 mA = 250 °C
The CO2 signal is scaled as: 0 mA = 0 % CO2;
20 mA = 20 % CO2
10.3 rs 232 serial communication
(232)
RS 232 communication is fitted as standard. This allows for
the SD card data to be remotely accessed and downloaded.
A PC with a serial (COM) port is required. Software is
required. Please contact MMM limited for further details.
10.4 ethernet communication (eth)
This is an option that must be specified at the point of
ordering.
An Ethernet port can be fitted to connect the incubator to a
network. This allows the SD card to be remotely accessed
and for the incubator screen to be displayed on a PC.
Connection to a PC network is required. Contact your IT
manager to see if this possible.
Instructions will be sent separately when this option is
ordered.
10.5 multi-door (8id)
This can be retro fitted.
The inner glass door can be replaced with a multi-door. This
has 8 smaller glass doors in a stainless steel frame.
By only opening one small door to access the sample you
require, less heat, humidity and CO2 are lost.
This improves recovery of the chamber back to ideal
conditions and reduces the amount of CO2 used.
10.2 4 - 20 ma retransmission (retx)
This is an option that must be specified at the point of
ordering.
This feature allows the chamber temperature and CO2
process values to be retransmitted as a 4 - 20 mA signal.
This 4 - 20 mA signal can be connected to external
equipment for independent validation of incubator
performance.
11accessories
This is the list of some of the accessories currently
available.
Please contact MMM if the part you require is not listed.
PTSK
Stacking kit for 2 x Comfort 190
PTST1
Wheeled platform for Comfort 190
PRV
Single stage CO2 pressure reducing valve
PRV2
Two stage CO2 pressure reducing valve
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
15
Instructions for use
RO6
In line CO2 reducing valve with gauge
(0.1 to 2 bar)
PNEU
Automatic change over unit for two CO2
cylinders
TYGON
Tygon gas tubing, per meter
12cleaning
Electrical hazard. Do not spill water or
cleaning fluids over the inner or outer
surfaces.
Disconnect before cleaning.
Completely dry the unit before
reconnecting the power.
12.3 oxidation at high temperature
(comfort 190s only)
When operated at 200 °C the action of atmospheric oxygen
may cause discolouration of the stainless steel surfaces.
This natural oxidation is not detrimental to the quality of the
incubator and is completely harmless.
13service
13.1 warranty
All MMM Comfort 190 incubators are supplied with a two
year manufacturer’s warranty from the date of installation.
To register your warranty, please fill in the User Guarantee
Registration Card and post to MMM Limited or to your local
distributor.
13.2 preventative maintenance
12.1 outer surfaces
Always disconnect the incubator before cleaning.
It is recommended that the exterior of the incubator regularly
wiped clean. Use a non-abrasive soft cloth dampened with a
warm soapy water solution.
This Culture Safe CO2 Incubator is built to last for many
years, but to ensure that the unit continues to perform
to the highest level we recommend that you purchase a
preventative maintenance contract from MMM.
The incubator should be calibrated annually.
Make sure the exterior is thoroughly dry after cleaning.
13.3 returning a unit
12.2 inner surfaces
Should your incubator require repair or maintenance then
contact your local distributor in the first instance.
It is advisable to clean the chamber regularly.
Returns to MMM will only be accepted with prior
authorisation from the Service Department.
Use a 70 % isopropanol 30 % distilled water solution.
Apply using a sterile cloth so as not to introduce any
contamination.
Never use any of the following:
• Any chlorine based detergents or bleaches.
• Any acidic cleaning agents.
• Sodium Azide
•Iodine
• Ferric Chloride
Wipe over the inner surfaces of the stainless chamber, all
the shelves including the underside of the shelves, the shelf
racks and the water tray.
The shelves, racking and water tray can be sterilised in an
autoclave if required.
Clean the glass door and the inner gasket seal.
Clean the inner stainless steel surfaces of the outer door
and the outer door gasket.
16
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
We recommend that you keep all packaging.
When returning a unit please include the following
information:
•
•
•
•
•
Incubator model and serial number
Date of purchase
Authorisation number
Name of local distributor if not purchased from MMM
A detailed description of the fault (we can never have too
much information)
• Where was the incubator located
• Contact details of the person who reported the fault
• A completed decontamination certificate (plus a copy
faxed through in advance)
MMM cannot accept any returns without a completed
decontamination certificate.
Instructions for use
14disposal
Volume
Number of shelves
The incubator must not be disposed of at public waste
collection points. It must only be disposed of by licensed
waste recycling companies.
Contact MMM for further information regarding WEEE
regulations.
15technical
information
15.1 technical data
Litres (cu. ft.) 190 (6.7)
4 shelves. Maximum of 8
SHIPPING DIMENSIONS
Width
Height
Depth
mm (inches)
mm (inches)
mm (inches)
925 (36.4)
1080 (42.5)
850 (33.5)
kg (lbs)
kg (lbs)
kg (lbs)
kg (lbs)
94.5 (208)
104 (229)
109 (240)
117.5 (259)
WEIGHT
Comfort 190, Net
Comfort 190S, Net
Comfort 190, Shipping
Comfort 190S, Shipping
15.2 dimensional drawings
TEMPERATURE MANAGEMENT
Range
°C
Control
Stability
Uniformity
Door recovery,30 s
opening
°C
°C
°C
minutes
5 °C above ambient to
60 °C
0.1 °C
±0.1 °C @ 37.0 °C
±0.25 °C @ 37.0 °C
13
Range
Control
Stability
Uniformity
% CO2
% CO2
% CO2
% CO2
0-20 %
±0.1 %
±0.2 %
±0.2 %
Door recovery, 30 s
opening
CO2 sensor type
CO2 inlet pressure
CO2 inlet connection type
minutes
6
CO2 MANAGEMENT
NDIR
Bar
0.3 to 0.7
4 mm push fit barbed type
HUMIDITY MANAGEMENT
Humidity control type
Range
Door recovery, 30 s
opening
Passive
% rH
minutes
90 % ± 5 %
50
Nominal voltage 50/60 Hz
V
230 1N ~PE
Power; Comfort 190
Power ; Comfort 190S
sterilise cycle
Power consumption
Main fuse F1; Comfort 190
Main fuse F1;
Comfort 190S
W
W
380
1800
ELECTRICAL DATA
kWh
0.058
6.3 A / 6.3 x 32mm / 250 V / TT
10 A / 6.3 x 32mm / 250 V / TT
EXTERIOR DIMENSIONS
Width
Height
Depth
Wall clearance, rear
Wall clearance, side
mm (inches)
mm (inches)
mm (inches)
mm (inches)
mm (inches)
765 (30.1)
862 (34)
734 (28.9)
100 (3.94)
50 (1.97)
INTERIOR DIMENSIONS
Width
Height
Depth
mm (inches)
mm (inches)
mm (inches)
632 (24.9)
686 (27)
440 (17.3)
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
17
Instructions for use
18
CO2CELL Comfort 190_np_en 1301_mmm_V1.02
Manufactured in the EU
MMM Medcenter Einrichtungen GmbH
Semmelweisstrasse 6
D-82152 Planegg
Germany
T. +49 89 89 92 26 20
F. +49 89 89 92 26 30
e-mail: medcenter@mmmgroup.com
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