User manual | AxioCam HR - Office of Research, Innovation and Economic

Microscopy from Carl Zeiss
AxioCam HR
Success Through Performance
The flexible high-end camera for
digital documentation and image analysis
Superior performance for research and
routine work – brilliant quality documentation
Increasingly complex applications in pathology, develop-
High-resolution for lossless
mental biology and material science demand microscope
digital microscopy
systems and camera technologies that reach the very lim-
Documenting with the AxioCam HR means seeing
its of what is physically possible. A Carl Zeiss camera that
what the microscope sees – in full microscope res-
meets even the highest demands of digital documentation
olution without interpolated image information.
has established itself in high-end microscopy. The mono-
Different resolutions can be set depending on the
chrome version is, ideally suited to Live Cell Imaging –
application: from 1388 x 1040 up to 4164 x 3120,
even at high speeds. The color variant can be used univer-
corresponding to 13 megapixels per color channel.
sally: in fields ranging from materials sciences and the
materials industry, pathology, cytology, hematology and
Outstanding image quality even
histology through to botany, zoology, forensics and phar-
with weak fluorescence
macology.
With a dynamic range of 1 : 2500 (at 12.5 MHz) and
a 14 bit gray level range, the monochrome version
of the AxioCam HR produces high-contrast images
even when it comes to demanding applications in
fluorescence microscopy. The Peltier-cooled camera
offers exposure times ranging from milliseconds up
to several minutes. The advantage: high-quality
images, even when signals are extremely weak.
Selectable read-out modes for
more flexibility
The option of selecting from two different readout speeds (12.5 MHz and 25 MHz), as required,
makes the AxioCam HR impressively versatile in
terms of the range of applications it can perform.
With the fast read-out mode of 25 MHz, live images of between 12 images/s (full resolution) and
33 images/s (reduced resolution) can be achieved.
The advantages this offers are simple adjustment of the sample and ergonomic focusing in all
situations. In conjunction with the AxioVision
Digital High Speed Recorder module or the
2
Systems with Carl Zeiss quality:
the complete solution with microscope,
camera and software
Axio Observer
SteREO Discovery
Axio Imager
Cell Observer® HS, fast time lapse or multidimen-
phototube or TV output. When combined with the
sional imaging is also possible with up to 48
Axio Imager or Axio Observer research microscopes
images/s using 5x5 binning, e.g. for the observa-
and the AxioVision imaging software, you will have
tion of extremely fast processes. Optimized fast
a high-performance system with a high degree of
acquisition of scanned resolutions can also be per-
automation – for reliable, reproduceable results.
formed. All in all, this means that the right setting
is available for every application.
Intelligent control with
AxioVision
Maximum convenience in a
AxioVision from Carl Zeiss is the software for all
compact format
requirements in digital imaging. It allows you to
Very small dimensions and no external control box:
control all the functions of both the camera and
the Zeiss Blue can be integrated as a compact solu-
microscope. Acquisition and processing, analysis
tion into any laboratory or working environment
and archiving – all your work procedures combined
and will not restrict your freedom of movement.
in a single platform. AxioVision is practice-oriented,
As the AxioCam HR does not have rotating fans,
intuitive in terms of operation and easily adapted to
it works without any vibrations and transfers the
your individual requirements. In addition, you can
digital image data directly to your computer with-
also present your image data in a meaningful
out interference.
way: the Unsharp Masking option allows even the
finest details to be displayed in high contrast on the
Perfect interaction in the
monitor.
overall system
Carl Zeiss offers a wide range of components that
complement each other perfectly and, when combined as an overall system, offer your applications optimum support. The AxioCam HR can be
connected to any Carl Zeiss microscope that has a
Highest image quality using microscanning
Acquisition time at short exposure times:
Resolution
AxioCam HRc
1388 x 1040
0.3 s
2776 x 2080
1.4 s
4164 x 3120
2.6 s
4164 x 3120 (Fast Scan)
0.6 s
AxioCam HRm
0.4 s
0.9 s
-
Loligo Pealei (squid), liver,
Stain: hematoxylin eosin,
David Patterson, Marine Biological Laboratory,
Woods Hole, Massachusetts, USA
3
Flexibility for every application:
color and monochrome
Color or monochrome:
High performance at low light
always the right choice
intensities
Routine tasks or individual applications – the
The extremely high sensitivity of the large
AxioCam HR from Carl Zeiss is the camera of
2/3" sensor, an outstanding signal to noise ratio
choice for the complete range of applications.
and Peltier cooling for long exposure times – these
Whether you use it as a versatile color camera or in
are the qualities that allow the AxioCam HR to
the monochrome variant with optional RGB filter
make high-quality imaging possible, even under
modules – the AxioCam HR delivers color images in
the most challenging lighting conditions.
extremely high resolution, for every application.
Monochrome CCD for
Color co-site Sampling for
fluorescence imaging
excellent color brilliance
Sensitive, weakly fluorescing specimens or contrast
Accurate color images of even the finest structures
and acquisition procedures in several dimensions
without color moiré: the large CCD sensor of the
require maximum resolution with exposure times
AxioCam HRc ensures perfect color accuracy. By
that are as short as possible. The monochrome ver-
scanning all the red, green and blue components of
sion of the AxioCam HR has been specially devel-
the image, the patented Color co-site Sampling
oped with an enhanced sensitivity range to cope
technique achieves a color brilliance that can other-
with these demanding tasks.
wise only be achieved using 3 sensors. It guarantees
you complete color information for every pixel – no
“approximated” interpolated colors.
Medicine
Appendix, Prof. Dr. Joachim Diebold,
Institute of Pathology at the University of Munich,
Germany
4
Applications
Peacock feather,
Martin Distel, Reinhard Köster,
GSF, Institute of Developmental Genetics,
Neuherberg, Germany
Superior performance without
color filters
Crucial for your results when working at the limits
of visibility: clear advantages in resolution and sensitivity thanks to a CCD sensor without light-reducing color filters.
Maximum performance for live cell
applications
• The spectrum of detectable light is extended
into the otherwise invisible near infrared.
A unique feature of the AxioCam HR is the possibility
of adjusting microscanning and the read-out speed.
• Even in the basic resolution of 1388 x 1040 pix-
This combination means that the AxioCam HR can be
els, images are acquired without the interpola-
used with a high degree of versatility – making it an
tion of a color sensor and the compromises
economical and individually adaptable solution that is
associated with this.
particularly attractive to research institutes which
• Images are scanned 4 x faster than with the
color camera.
carry out many different live cell applications. It is only
in combination with the Cell Observer® system, how-
• The size of the file is reduced to a third com-
ever, that you can fully exploit the possibilities of the
pared with the AxioCam HRc color camera.
camera – especially those offered by the monochrome version. Integration into AxioVision enables
Full-strength signals
you to perform every conceivable application in Live
With the monochrome AxioCam HRm, no optical
Cell Imaging – from extremely fast, one-dimensional
elements such as color or infrared filters stand in
time lapse imaging and individual images in multi-
the way of the light. This means that even fluo-
channel fluorescence applications through to the
rochromes that emit in the near infrared are
combination of fast time lapse images with multi-
detected by the AxioCam HRm.
dimensional acquisition – all with just one camera.
Biology
Materials Analysis
5
Brilliantly sharp color images
thanks to Color co-site Sampling
With ordinary 1-chip digital cameras, color images
distance of one pixel, meaning that each point is
are acquired with a sensor. Each pixel of this sensor
seen by the sensor at least once in all colors. Inter-
is sensitive to just one of the three basic colors. As
polated color information is therefore not needed. In
only one image is acquired, each sensor pixel receives
this way, the complete color information for each
only the color information for red, green or blue at
detail is obtained in four images and put together to
any one point in the image. However, as all three
form one image that is identically sharp in all three
color channels are required simultaneously at a sin-
color channels.
gle point in the image in order to display a pixel in
color, the missing color channels are determined by
Microscanning for all details
means of interpolation from the nearest neighbor-
Using the same procedure you can achieve even
ing pixels. This approximation results in the genera-
more, however: by acquiring images at additional
tion of imperfect images which display disruptive
positions in the spaces between pixels, the accura-
color fringes and distorted colors at fine image struc-
cy of the images produced by the AxioCam HR
tures and edges.
is increased again, also in case of the monochrome
camera. With up to three additional positions on
With the Color co-site Sampling technique used by
the x and y axes, the resolution is increased from
the AxioCam HR, several images of a specimen are
1388 x 1040 by a factor of 9 up to 4164 x 3120 pix-
produced and combined into a sharp resulting
els. The Color co-site Sampling used at the same
image. After the acquisition of each image a piezo
time ensures perfect, color-correct reproduction of
mechanism moves the sensor by precisely the
the finest structures.
The resolving power of Carl Zeiss objectives in the intermediate image with 0.63x and
1.0x TV adapters in combination with the AxioCam HR's 2/3” CCD sensor (8.5 mm x 6.4 mm).
Objective
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
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Magnification
EC Plan-NEOFLUAR
FLUAR
EC Plan-NEOFLUAR
ACHROPLAN/N-ACHROPLAN
FLUAR
EC Plan-NEOFLUAR
Plan-APOCHROMAT
LCI Plan-NEOFLUAR
EC Plan-NEOFLUAR
EC Plan-NEOFLUAR
Plan-APOCHROMAT
EC Epiplan-NEOFLUAR
Plan-APOCHROMAT
1.25
2.5
5
10
10
20
20
25
40
40
63
100
100
NA
0.035
0.12
0.15
0.25
0.5
0.5
0.75
0.80
0.75
1.3
1.4
0.9
1.4
Lp/mm
(TV-Cpl 1.0x)
96
144
90
75
150
75
113
96
56
98
67
27
42
Necessary
camera resolution
1632
2448
1530
1275
2550
1275
1921
1632
952
1666
1139
459
714
x
x
x
x
x
x
x
x
x
x
x
x
x
1229
1843
1152
960
1920
960
1446
1229
717
1254
858
346
538
Lp/mm
(TV-Cpl 0.63x)
152
229
143
119
238
119
179
152
89
155
106
43
67
Necessary
camera resolution
2584
3893
2431
2023
4046
2023
3040
2584
1513
2635
1802
731
1139
x
x
x
x
x
x
x
x
x
x
x
x
x
1946
2931
1830
1523
3046
1523
2291
1946
1139
1984
1357
550
858
Technology
View through the C-mount connection and
the infrared barrier filter on the high-resolution CCD sensor
Color co-site Sampling
Single shot image with
chromatic aberrations at
the edges
Color co-site Sampling with
exact color reproduction
During acquisition, the sensor is moved by the distance of one pixel.
As a result of this every pixel is scanned at least once in all colors and
the sharpness is therefore identical in all three color channels
Microscanning
1.3 megapixel image
High-resolution image
using the AxioCam HR
with 13 megapixels
Moving the sensor along the vertical and horizontal axes supplies
additional information about image details. The basic resolution
increases as a result up to 4164 x 3120 pixels (13 megapixels)
You will find an animated version of this illustration on our internet site.
7
AxioCam HR: Data and Facts
The data and facts apply equally to the AxioCam HRm and AxioCam HRc. Where different data apply, this will be pointed out.
Sensor
Maximum camera resolution
CCD basic resolution
Pixel size
Sensor size
Spectral sensitivity
Dynamic range
Full well
Dark current
Resolution improvement
Selectable resolution
AxioCam HRm
Selectable resolution
AxioCam HRc
Live image frame rates
AxioCam HRm
Live image frame rates
AxioCam HRc
Fast readout modes
in AxioVision module
Fast Acquisition
AxioCam HRm and AxioCam HRc: Sony ICX 285, progressive readout,
AxioCam HRc with RGB filter mask
4164 x 3120
1388 x 1040 = 1.4 megapixels
6.45 µm (h) x 6.45 µm (v)
Chip area 8.9 mm x 6.7 mm, equivalent 2/3"
AxioCam HRm: approx. 350 nm-1000 nm with BK 7 protection glass
AxioCam HRc: approx. 400 nm-700 nm with BG 40 IR barrier filter
Typical > 1: 2200 at 25 Mhz at < 7.7 e readout noise
Typical > 1: 2500 at 12.5 Mhz at < 6.8 e readout noise
Typical 17.000 e
Typical 0.7 e/pixels/s, dark durrent compensation for maximum low light
performance at long exposure times
Microscanning technology enables a configurable image resolution
beyond the basic sensor resolution
HxV
H x V (Microscanning Mode)
276 x 208 Binning 5 x 5
2776 x 2080
346 x 260 Binning 4 x 4
4164 x 3120
462 x 346 Binning 3 x 3
694 x 520 Binning 2 x 2
1388 x 1040, Single Shot
HxV
H x V (Microscanning Mode)
276 x 208 Binning 5 x 5, RBG
1388 x 1040 Color co-site Sampling
346 x 260 Binning 4 x 4, B/W
2776 x 2080 Color co-site Sampling
462 x 346 Binning 3 x 3, RGB
4164 x 3120 Color co-site Sampling
694 x 520 Binning 2 x 2, B/W
4164 x 3120 Fast Scan
1388 x 1040, Single Shot
HxV
Mode/Binning
Max. frame rate (25 MHz)*
1388 x 1040
Slow/1
12 images/s
694 x 520
Middle/2
21 images/s
462 x 346
Fast/3
31 images/s
HxV
Mode/Binning
Max. frame rate (25 MHz)*
1388 x 1040
Slow/1
12 images/s
460 x 344
Middle/3
26 images/s
276 x 208
Fast/5
33 images/s (Binning in color)
• Five preloadable exposure time parameters in camera head enable
especially high speed multichannel acquisition
• Continuous mode enables fast time lapse acquisition
• Overlapping exposure and readout enables maximum frame rates for time
lapse images and minimizes acquisition time for microscanning imaging
Hard disk recording
Readout of sub frames (ROI)
Signal amplification
Digitization
CCD cooling
Interface
Range of integration time
Signal output connectors
Optical interface
Max. file size per image
Operating system
Housing
Registration
Power supply
Ambient conditions
(operation)
Ambient conditions
(storage)
Order number
All specifications are subject to change without notice.
* Frame rates depend on exposure time, readout mode and PC hardware
Using Microscanning it is possible to generate high-resolution images by combining several individual
images for which the sensor has been shifted by extremely small distances (subpixel dimensions) in
each case.
Color co-site Sampling allows optically identical resolution in each of the three RGB color channels. Color
interpolation does not take place.
Relative Spectral Sensitivity AxioCam HRm
Relative Spectral Sensitivity AxioCam HRc with BG 40 IR-Filter
1.2
1.0
1
Relative Response
0.8
0.6
0.4
0.2
0.8
0.6
0.4
0.2
0.0
430
460
490
520
550
590
Wavelength in nm
Carl Zeiss MicroImaging GmbH
07740 Jena, Germany
BioSciences | Göttingen Location
Phone: +49 551 5060 660
Telefax: +49 551 5060 464
E-Mail: [email protected]
www.zeiss.de/axiocam
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670
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380
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680
740
800
860
920
980
48-0059/e – printed 03.08
0
400
Information subject to change.
Printed on environmentally friendly
paper bleached without cholorine.
Relative Response
Inline recording of image data directly to hard disk at all speeds with
AxioVision modul Fast Acquisition available
Random definition of regions of interest (ROI) on the sensor enables
further increase of achievable frame rates
Analog: 2x, digital 32x
Two switchable readout speeds offer different depth of digitization
High Quality: 14 bit/12.5 Mhz and High Speed: 12 bit/25 Mhz
Single stage Peltier cooling, regulated
FireWire IEEE 1394a (400 megabits/s)
1 ms up to 600 s
2x TTL-Out: exposure time, readout time (i.e. for driving external
electric shutters), 1x Trigger-In to start an acquisition
C-Mount, max. 5 mm free back focal depth
Approx. 78 MB with 4164 x 3100 pixels at 14 bit (monochrom)
and 3 x 14 bits (color)
Microsoft® Windows 2000 Professional SP4, Microsoft® Windows XP
Professional SP2, Microsoft® Windows Vista Ultimate
Blue anodized aluminum, with cooling fins, 1/4" connection for tripod
mount, 11 cm x 8 cm x 6.5 cm, 500 g
CE, cUL
10-33 V, DC, 5W, power supply provided by FireWire bus (external
power supply only for Notebook operation required)
+5° ... +35° Celsius, 10% ... 80% relative humidity, no condensation,
free air circulation required
-15° ... +60° Celsius, 90% relative humidity at 40° Celsius, 80% relative
humidity at +20° Celsius, no condensation
AxioCam HRm: 426511-9901-000
AxioCam HRc: 426510-9901-000
Wavelength in nm

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