In touch with life 1. Pipetting and Dispensing “We care for you and your pipettes by providing PhysioCare ergonomics and epServices” 26 Pipetting and dispensing 1 | Info | Pipetting | Dispensing Summary of applications Application Product Page Manual pipetting Instruments Single-channel, combined tip ejection and pipetting button Eppendorf Reference 45 Single-channel, separate tip ejection button Eppendorf Research®, Research plus 33 Multichannel, separate tip ejection button Eppendorf Research®, Research plus 33 Single-channel, combined tip ejection and pipetting button, positive displacement BioMaster Positive Displacement Pipette 63 Single-channel, combined tip ejection and pipetting button, can be used as either a positive-displacement or an air-cushion pipette Maxipettor® 64 Electronic pipetting, single-channel and multichannel, separate tip ejection button Eppendorf Research pro 48 Pipet fillers for use with serological pipets Easypet®/Pipet Helper® 73 Standard application epT.I.P.S. Bulk/Racks/Reloads 53 Standard application, tips for Maxipettor Maxitip P, Maxitip S 64 Contamination-free pipetting ep Dualfilter T.I.P.S. 58 Consumables Contamination-free pipetting, tips for Positive Displacement Pipette Positive Displacement Pipette Tips 63 Pipetting with the highest level of biological purity epT.I.P.S. Racks/Singles, Eppendorf Biopur® 60 Applications with samples that contain detergents epT.I.P.S. LoRetention 56 Manual dispensing, single-channel, positive displacement, contamination-free Repeater® Plus 65 Electronic dispensing, single-channel, positive displacement, contamination-free Repeater stream/Xstream 65 Electronic dispensing, single-channel and multichannel separate tip ejection button Eppendorf Research® pro 48 Tips for Repeater Plus and Repeater stream/Xstream, contamination-free, standard application and highest level of biological purity Combitips Plus®, Standard, Sterile and Biopur 68 Tips for Eppendorf Research pro Contamination-free epT.I.P.S. ep Dualfilter T.I.P.S. 58 Preventive Maintenance QuickCheck PMSM 30 Pipette Calibration Advantage Calibration 31 Pipette Calibration and Certification Advantage Certification 31 Custom Calibration and Certification GLP/GMP Custom Calibration 31 Manual dispensing (dispensing of partial volumes) Instruments Consumables Service products for manual pipetting Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipetting and dispensing 27 Application Product Page Fully automated, electronic pipetting and dispensing, single-channel and multichannel epMotion® 5070 and 5075 automated pipetting systems 76 All routine pipetting, sample and reagent transfer, real-time PCR set-up, cell-based and immunoassay set-up epMotion 5070 epMotion 5075 LH 78 82 Cell culture and cell based assays in a hood epMotion 5070 CB 80 Walk-away nucleic acid purification for MagBead protocols epMotion 5075 TMX 86 Walk-away nucleic acid extraction with ready-to-go protocols filtration & SPE, in vitro plasmid amplification epMotion 5075 VAC 84 Fully automated PCR: set-up and cycling epMotion 5075 MC 88 Tips for epMotion workstations, standard application epT.I.P.S. Motion 97 Tips for epMotion workstations, contamination-free applications epT.I.P.S. Motion Filter 97 Performance Plans for epMotion Preventive Maintenance Advantage Service Premium Service 100 100 100 Calibration for epMotion dispensing tools Advantage epMotion Tool Certification epMotion Tool GLP/GMP Custom 101 101 Automated pipetting and dispensing Instruments Consumables Service products for epMotion automated pipetting systems Eppendorf purity levels 55 The preceding table shows a selection of applications for pipetting and dispensing and serves as an overview. The complete product portfolio of Eppendorf Liquid Handling Systems is found on the following pages. All Pipetting and Dispensing products shown on the following pages can be provided as Customized Solutions. Further information on page 256. Eppendorf epGreen Eppendorf recognizes its responsibility as a global citizen and is striving to reduce the environmental impact of its business operations and the products we produce, as well as to support the sustainable green initiatives of our customers. In this section you will find products with special epGreen features. customized products p. 256 For more information on epGreen, see pages 312 and 313. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Info | Pipetting | Dispensing Summary of applications 28 Pipetting and dispensing Product selection guide for pipetting and dispensing 1 | Info | Pipetting | Dispensing Volumes 0.1 µl—1,250 µl Aqueous solutions Single dispensing Multiple dispensing Liquids with high vapor pressure and/or high viscosity Single dispensing Multiple dispensing Instruments Page Consumables Page Eppendorf Research®1 40 epT.I.P.S. 53 Eppendorf Research plus 33 epT.I.P.S. 53 Eppendorf Reference2 45 epT.I.P.S. 53 Eppendorf Research pro3 48 epT.I.P.S. 53 BioMaster Positive Displacement Pipette 63 Positive Displacement Pipette Tips 63 Repeater® stream 65 Combitips® Plus4 68 Repeater Xstream 65 Combitips Plus 68 epMotion® 76 epT.I.P.S. Motion 97 Repeater Plus5 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Eppendorf Research pro 48 epT.I.P.S. 53 epMotion 76 epT.I.P.S. Motion 97 BioMaster Positive Displacement Pipette 63 Positive Displacement Pipette Tips 63 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater Plus 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Volumes up to 25 ml Aqueous solutions Single dispensing Multiple dispensing Liquids with high vapor pressure and/or high viscosity Instruments Page Consumables Page Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Easypet®/Pipet Helper® 73 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Single dispensing Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Multiple dispensing Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 U.S. Pats. 5,531,131/6,199,435 U.S. Pat. 5,511,433 U.S. Pats. 6,499,365/6,778,917 4 U.S. Pats. 5,620,660/5,620,661 5 U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,729 1 2 3 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipetting and dispensing 29 Volumes 1,250 μl–2,500 μl Volumes 2.5 ml–10 ml Instruments Page Consumables Page Instruments Page Consumables Page Eppendorf Research®1 40 epT.I.P.S. 53 Eppendorf Research 40 epT.I.P.S. 53 Eppendorf Research plus 33 epT.I.P.S. 53 Eppendorf Research plus 33 epT.I.P.S. 53 Eppendorf Reference2 45 epT.I.P.S. 53 Repeater stream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Easypet/Pipet Helper 73 Easypet®/Pipet Helper® 73 Maxipettor 64 Maxitip P, Maxitip S 64 64 ® Maxipettor® 64 ®3 Maxitip P 64 Repeater Plus4 65 Combitips Plus 68 Repeater Plus 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Maxipettor 64 Maxitip P 64 Maxipettor 64 Maxitip P, Maxitip S 64 64 Repeater Plus 65 Combitips Plus 68 Repeater Plus 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Volumes up to 50 ml Instruments Page Consumables Page Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Easypet®/Pipet Helper® 73 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 Repeater stream 65 Combitips Plus 68 Repeater Xstream 65 Combitips Plus 68 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Info | Pipetting | Dispensing Product selection guide for pipetting and dispensing 30 Service products 1 | Service | Pipetting | Dispensing Pipette Calibration and Repair Services For over 65 years Eppendorf has been an innovator and leading provider of liquid handling systems, which include pipetting devices and consumables. This total approach philosophy extends beyond products to our quality pipette calibration, certification, maintenance and repair services— for all major brands of pipettes, not just our own. We offer a range of service options from basic calibration at an economical price to GLP/GMP custom-designed calibration services. Your results depend on the integrity of your pipettes. Trust in Eppendorf, your satisfaction is guaranteed! We deliver on our promises and your peace of mind is our utmost concern. We approach every pipette as the instrument of your success. We know the pipette we service today will be needed for a breakthrough discovery or critical result. It has to be right. Attention to detail and exceptional quality and care highlight every plan we offer. Speed. Accuracy. Reliability. ‡‡ Full flexibility—any mix to meet your needs – Our value plans for your custom protocols – One lab or global multinational service – On site and in our state of the art facilities ‡‡ Full service consultation services – Technician training and seminars – Custom protocol development – Documentation management – PhysioCare® ergonomics and pipette clinics ‡‡ Fully equipped and trained calibration professionals – Complete on site calibration and repair available – Proprietary Eppendorf calibration software – Environmentally robust, semi-micro, NIST traceable balances – ISO 9001:2000 Registered Get started today at: www.pipettecalibration.com Procedures QuickCheck PMSM Internal and external inspection of pipette 3 Internal and external cleaning of pipette 3 Replacement of worn or broken parts, if needed* 3 Pipette calibration performed using NIST-traceable, semi-micro (0.00001g) balance – Number of measuring points single-channel, fixed-volume: – single-channel, adjustable-volume: – multichannel: – Calibration label applied to pipette – Individual weighing results, mean volume, inaccuracy and imprecision values measured: – recorded: – Calibration report supplied – Single-channel manual or electronic pipette* List USD $20.00 + shipping Catalog No. 901510186 Multichannel manual or electronic pipette* List USD $48.00 + shipping Catalog No. 901510187 *1 Handgrip, motor and PCB are priced separately. *2 Is included in the 10 measurements *3 Standard value, deviations possible, excl. shipping duration *4 Other services may be available upon request Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. 31 1 | Service | Pipetting | Dispensing Service products Online epExpressSM Pipette Calibration Center Now it’s easier than ever to send us your pipettes for calibration and repair. Visit our new online epExpress Pipette Calibration Center at www.pipettecalibration.com. Here you can complete our online Service Authorization Form and get a real-time quotation based on your specific calibration service requirements. As a registered Eppendorf North America pipette calibration/ repair customer, you will not only enjoy fast and easy online ordering, but also gain access to a private log-in area where you can: iew calibration and repair service history V for individual pipettes ‡‡ Get reminders for pipettes that are coming due for calibration ‡‡ View your mail-in pipette service order history ‡‡ And more! ‡‡ For more information visit www.pipettecalibration.com Advantage Calibration Advantage Certification GLP/GMP Custom Calibration 3 3 3 3 3 3 3 3 3 3 3 3 8 8 4 low/4 high 4 low/4 high 1 reading per channel low/high 1 reading per channel low/high As requested, based on customer requirements; may include “as found” data 3 3 3 3 3 3 – 3 3 – 3 3 $25.00 + shipping $35.00 + shipping 901510184 901510180 $60.00 + shipping $75.00 + shipping 901510185 901510181 Determined based upon level of service required Determined based upon level of service required In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 32 Ergonomics 1 | Instruments | Pipetting | Dispensing PhysioCare Concept® Changing the face of pipetting. The Eppendorf PhysioCare Concept: The new standard for integrated liquid handling systems and work processes The Eppendorf PhysioCare Concept (PCC) differentiates Eppendorf® pipettes and hand dispensers from other brands by addressing all relevant aspects of pipetting: PhysioCare focuses on the whole person, not just the human hand or the device. And there are very specific criteria that our pipettes must fulfill— see our eight points below. When you take all of these factors into account, it’s evident that Eppendorf offers the most powerful liquid handling systems on the market today. All Eppendorf PhysioCare Concept pipettes fulfill the following eight criteria: ‡‡ Integrated systems Complete pipette and pipette tip program to fit any application and budget. ‡‡ Exceptional durability Made of state-of-the-art materials, up to three-year warranty. ‡‡ Optimized form and function Easy operation throughout all work steps, even for complex pipetting tasks. ‡‡ Perfectly balanced Designed to fit comfortably in the hand, with models weighing up to 30% less than other brands (see graph on page 34). ‡‡ Intuitive operation Color-coded pipette-and-tip matching system for easy identification saves time and streamlines tasks. ‡‡ Minimal user exertion Designed to minimize stress, with manual models requiring up to 50% less pipetting force than other brands (see graph on page 35). ‡‡ Simple maintenance Easy to clean and repair, reducing maintenance time and related expenses. ‡‡ Total support Eppendorf PCC pipettes arrive certified and ready to use; reliable calibration service and maintenance is available through Eppendorf North America Services (see pages 30 and 31). For more information visit: www.physiocare-concept.info Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 33 NEW! Eppendorf Research® plus 1 | Instruments | Pipetting | Dispensing NEW! Eppendorf sets a new standard in pipetting. The ultra light Eppendorf Research plus pipette meets the highest needs in precision and accuracy—combined with ultimate ergonomics and increased flexibility. It is a good feeling to know you are working with one of the most advanced pipettes in the world. A spring loaded tip cone, a secondary adjustment option, an improved volume display—and all that in an ultra light, fully autoclavable pipette: That is the new Eppendorf Research plus pipette. Ergonomics: Feel the difference in weight, pipetting forces and the spring loaded tip cone. Flexibility: Your next experiment will need new pipetting demands. Your new laboratory might have different sources of contamination. Your new pipette should offer all the flexibility you need. Adjust your Research plus to your needs, autoclave the entire pipette or only the lower part. Choose among singlechannel, multichannel and fix-volume pipettes in different sizes. Robustness: Durability was tested thoroughly and has met our highest quality requirements. Made with space-age materials, resistance to chemical, thermal and mechanical stress is now continuously achieved! In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 34 Adjustable- and fixed-volume pipettes 1 | Instruments | Pipetting | Dispensing NEW! Eppendorf Research® plus An Eppendorf Research plus pipette is of extremely low weight! This can only be achieved by using the most advanced materials and production processes, including the new Eppendorf PerfectPiston™ system, made of Fortron®. Fortron is an organic polymer with impressive characteristics. It is highly resistant to heat, acids and alkalies, mildew, bleaches, aging, sunlight and abrasion. It absorbs only small amounts of solvents and resists dyeing. This means your pipette is safe, robust and ultra light. Eppendorf Research® plus ≤10 µl 10–100 µl 100–1000 µl Competitor Br Competitor Bi Competitor Gn Competitor R Competitor RL Competitor F 50 g 60 g 70 g 80 g 90 g 100 g 110 g 120 g 130 g ‡ See the low weight of the Research plus—no matter which size. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 35 1 | Instruments | Pipetting | Dispensing NEW! Eppendorf Research® plus Attachment force Achieve optimal tightness with minimal attachment forces with the Eppendorf Research plus. The spring loaded tip cone helps to reduce stress without sacrificing tightness. Just give it a try—you will love it! Starting forces (1000 µl) Ending forces (1000 µl) Eppendorf Research® plus Tip ejection forces How many tips do you use per day? Even small differences in the tip ejection forces make a big change if you do it day by day. The tip ejection forces of the Eppendorf Research plus can be as low as only 3.6 N, depending on the size and the force you use to fix the tip. Positioning of control elements The positions and shapes of all control buttons and displays have been optimized again to ensure stress-free workflow and intuitive usage. The volume adjustment wheel needs only a few turnarounds from maximum volume to minimum volume. Competitor Br Competitor Bi Competitor Gn Competitor R Competitor RL Competitor F 1N 2N 4N 6N 8N 10 N 12 N 14 N ‡ Pipetting forces are optimized to reduce stress. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 36 Adjustable- and fixed-volume pipettes 1 | Instruments | Pipetting | Dispensing NEW! Eppendorf Research® plus ‡Volume adjustment: – Only a few turns to reach your desired volume – Designed for one-handed operation ‡Fully autoclavable: – For easy decontamination ‡Lowest operation and tip ejection forces: – Reduces daily stress and fatigue ‡Volume display: – 4 digits – Magnifying shape ‡Secondary adjustment option for various liquid classes: – Adjust your pipette in seconds for better accuracy with liquids very different from water EP1759768, US 7, 434 484 and foreign counterparts and patents pending. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 37 ‡Channel indicator: – Use the pipette the same way round—all the time ‡Remove any of the independent channels: – Gain flexibility for individual needs ‡Spring loaded tip cone: – Improved ergonomics – Tight fit to the tip – Independent suspension in each channel ‡Ultra lightweight with space-age materials: – Eppendorf PerfectPiston™ system made of Fortron®, chemically resistant for everyday use with aggressive solvents ‡Quick connection clip: – Remove lower part in just 2 steps! ‡Spring loaded tip cone: – Improved ergonomics for consistent tip attachment and ejection – Guarantees tight fit to the tip In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing NEW! Eppendorf Research® plus 38 Adjustable- and fixed-volume pipettes NEW! Eppendorf Research® plus 1 | Instruments | Pipetting | Dispensing Eppendorf Research plus (adjustable) Volume range Volume Inaccuracy1 Imprecision1 Catalog No. List USD ≤12.0% ≤6.0% ≤1.5% ≤0.7% 3120000011 $329.00 ≤5.0% 3120000020 329.00 3120000097 329.00 3120000038 329.00 3120000046 329.00 3120000054 329.00 3120000100 329.00 3120000062 329.00 3120000070 329.00 3120000089 329.00 Dark grey operating button, for use with 10 µl pipette tips 0.1–2.5 µl 0.1 µl 0.25 µl 1.25 µl 2.5 µl ±48.0% ±12.0% ±2.5% ±1.4% Grey operating button, for use with 20 µl pipette tips 0.5–10 µl 0.5 µl ±8.0% 1 µl ±2.5% ≤1.8% 5 µl ±1.5% ≤0.8% 10 µl ±1.0% ≤0.4% Light grey operating button, for use with 20 µl L pipette tips 2–20 µl 2 µl ±5.0% ≤1.5% 10 µl ±1.2% ≤0.6% 20 µl ±1.0% ≤0.3% Yellow operating button, for use with 200 µl pipette tips 2–20 µl 10–100 µl 20–200 µl 2 µl ±5.0% ≤1.5% 10 µl ±1.2% ≤0.6% 20 µl ±1.0% ≤0.3% 10 µl ±3.0% ≤1.0% 50 µl ±1.0% ≤0.3% 100 µl ±0.8% ≤0.2% 20 µl ±2.5% ≤0.7% 100 µl ±1.0% ≤0.3% 200 µl ±0.6% ≤0.2% Orange operating button, for use with 300 µl pipette tips 30–300 µl 30 µl ±2.5% ≤0.7% 150 µl ±1.0% ≤0.3% 300 µl ±0.6% ≤0.2% Blue operating button, for use with 1,000 µl pipette tips 100–1,000 µl 100 µl ±3.0% ≤0.6% 500 µl ±1.0% ≤0.2% 1,000 µl ±0.6% ≤0.2% Violet operating button, for use with 5 ml pipette tips 0.5–5 ml 0.5 ml ±2.4% ≤0.6% 2.5 ml ±1.2% ≤0.25% 5 ml ±0.6% ≤0.15% Turquoise operating button, for use with 10 ml pipette tips 1–10 ml 1 ml ±3.0% ≤0.6% 5 ml ±0.8% ≤0.2% 10 ml ±0.6% ≤0.15% Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 39 NEW! Eppendorf Research® plus Volume range Volume Inaccuracy1 Catalog No. Imprecision1 8-channel Catalog No. List USD 12-channel List USD Grey operating button, for use with 20 µl pipette tips 0.5–10 µl ±12.0% ±8.0% ±4.0% ±2.0% 0.5 µl 1 µl 5 µl 10 µl ≤8.0% ≤5.0% ≤2.0% ≤1.0% 3122000019 $787.00 3122000027 $912.00 3122000035 787.00 3122000043 912.00 3122000051 787.00 3122000060 912.00 Yellow operating button, for use with 200 µl pipette tips 10–100 µl 10 µl ±3.0% ≤2.0% 50 µl ±1.0% ≤0.8% 100 µl ±0.8% ≤0.3% Orange operating button, for use with 300 µl pipette tips 30–300 µl 30 µl ±3.0% ≤1.0% 150 µl ±1.0% ≤0.5% 300 µl ±0.6% ≤0.3% Eppendorf Research plus (fixed volume) Volume Inaccuracy1 Imprecision1 Catalog No. List USD ≤0.6% 3121000015 $223.00 3121000031 223.00 Grey operating button, for use with 20 µl pipette tips 10 µl ±1.2% Light grey operating button, for use with 20 µl L pipette tips 20 µl ±0.8% ≤0.3% ±1.0% ≤0.8% ±0.8% ≤0.3% Yellow operating button, for use with 200 µl pipette tips 10 µl ±1.2% ≤0.6% 3121000023 223.00 20 µl ±1.0% ≤0.3% 3121000040 223.00 25 µl ±1.0% ≤0.3% 3121000058 223.00 50 µl ±0.7% ≤0.3% 3121000066 223.00 100 µl ±0.6% ≤0.2% 3121000074 223.00 200 µl ±0.6% ≤0.2% 3121000082 223.00 Blue operating button, for use with 1,000 µl pipette tips 1 200 µl ±0.6% ≤0.2% 3121000090 223.00 250 µl ±0.6% ≤0.2% 3121000104 223.00 500 µl ±0.6% ≤0.2% 3121000112 223.00 1,000 µl ±0.6% ≤0.2% 3121000120 223.00 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Eppendorf Research plus (adjustable, multichannel) 40 Adjustable- and fixed-volume pipettes 1 | Instruments | Pipetting | Dispensing Eppendorf Research® fixed/adjustable/multichannel pipettes Product features Two-button operation Autoclavable lower part ‡‡ TÜV award: ergonomic, user-friendly and user-tested ‡‡ Viable calibration seal indicates factory calibration has not been changed ‡‡ Chemically resistant ‡‡ Reduced operating forces ‡‡ Single-handed operation ‡‡ Four-digit display ‡‡ Maintenance-free nose cones ‡‡ 3-year warranty ‡‡ ‡‡ Description The separate tip ejection (two-button operation) and TÜV Rheinland-certified ergonomics make our Eppendorf Research* pipettes a lab favorite all over the world. They are available in fixed-volume, adjustable-volume and multichannel versions to satisfy all requirements. Display The four-digit volume display is magnified for improved visibility; adjustable-volume models feature easy, single-handed volume setting. * U.S. Pats. 5,531,131/6,199,435 Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 41 1 | Instruments | Pipetting | Dispensing Eppendorf Research® fixed-volume pipettes The lower part can be easily removed for cleaning, autoclaving and decontamination. Ordering information: Eppendorf Research fixed-volume pipettes1 Volume Inaccuracy2 Imprecision2 Catalog No. List USD Yellow operating button, for use with 200 μl pipette tips 10 µl ±1.2% ≤0.6% 022443054 20 µl ±1.0% ≤0.3% 022443101 $215.00 215.00 25 µl ±1.0% ≤0.3% 022443151 215.00 50 µl ±0.7% ≤0.3% 022443208 215.00 100 µl ±0.6% ≤0.2% 022443259 215.00 Blue operating button, for use with 1,000 μl pipette tips 1 2 200 µl ±0.6% ≤0.2% 022443305 215.00 250 µl ±0.6% ≤0.2% 022443356 215.00 500 µl ±0.6% ≤0.2% 022443402 215.00 1,000 µl ±0.6% ≤0.2% 022443453 215.00 U.S. Pat. 6,119,435 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. For pipette service and maintenance information, see page 30; pipette tips information begins on page 53. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 42 Adjustable- and fixed-volume pipettes Eppendorf Research® adjustable-volume pipettes 1 | Instruments | Pipetting | Dispensing This space-saving pipette holder can be attached to a shelf or wall, ensuring that your pipette is always within easy reach. (022444913) Each Eppendorf Research adjustable-volume pipette (except for Research 10 ml) contains a service kit and an epT.I.P.S. Box with the appropriate volume tip. For pipette service and maintenance information, see page 30; pipette tips information begins on page 53. Ordering information: Eppendorf Research adjustable-volume pipettes1 Volume range Volume increment Volume Inaccuracy2 Imprecision2 Catalog No. List USD ≤6.0% ≤1.5% ≤0.7% 022471856 $318.00 Dark grey operating button, for use with 10 μl pipette tips 0.1–2.5 µl 0.002 µl 0.25 µl 1.25 µl 2.5 µl Grey operating button, for use with 20 μl pipette tips 0.5–10 µl 0.01 µl 1 µl ±2.5% 5 µl ±1.5% 10 µl ±1.0% Yellow operating button, for use with 200 μl pipette tips ≤1.8% ≤0.8% ≤0.4% 022471902 318.00 2.0–20 µl 0.02 µl 2 µl ±5,0% 10 µl ±1.2% 20 µl ±1.0% 10–100 µl 0.1 µl 10 µl ±3.0% 50 µl ±1.0% 100 µl ±0.8% 20–200 µl 0.2 µl 20 µl ±2.5% 100 µl ±1.0% 200 µl ±0.6% Blue operating button, for use with 1,000 μl pipette tips ≤1.5% ≤0.6% ≤0.3% ≤1.0% ≤0.3% ≤0.2% ≤0.7% ≤0.3% ≤0.2% 022471953 318.00 022472003 318.00 022472054 318.00 100–1,000 µl 0.1 µl ≤0.6% ≤0.2% ≤0.2% 022472101 318.00 500–5,000 µl 5 µl 500 µl ±2.4% 2,500 µl ±1.2% 5,000 µl ±0.6% Turquoise operating button, for use with 10 ml pipette tips ≤0.6% ≤0.25% ≤0.15% 022472151 318.00 1–10 ml 10 µl ≤0.6% ≤0.2% ≤0.15% 022472208 318.00 100 µl 500 µl 1,000 µl Violet operating button, for use with 5 ml pipette tips 1 2 1 ml 5 ml 10 ml ±12.0% ±2.5% ±1.4% ±3.0% ±1.0% ±0.6% ±3.0% ±0.8% ±0.6% U.S. Pat. 5,531,131 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 43 1 | Instruments | Pipetting | Dispensing epResearch3 Pack epResearch3 Pack includes: ‡‡ ption 1: O 3 Eppendorf Research® PhysioCare Concept® pipettes (0.5–10 µl, 10–100 µl, 100–1,000 µl); 3 refillable epT.I.P.S. boxes with 96 tips each; 3 Pipette Holders; SOP and Eppendorf pipette pen! ‡‡ Option 2: 3 Eppendorf Research PhysioCare Concept pipettes (2–20 µl, 20–200 µl, 100–1,000 µl); 3 refillable epT.I.P.S. boxes with 96 tips each; 3 Pipette Holders; SOP and Eppendorf pipette pen! Description Comfortable, high precision pipetting is the result of perfect teamwork between the pipette and tip. Why not place your trust in the unbeatable team of Eppendorf Research pipettes and the epT.I.P.S. pipette tips? Now conveniently available as epResearch3 Pack at a special price that’s too good to miss! Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Ordering information Description Catalog No. List USD epResearch Pack 1, (10, 100 and 1,000 µl pipettes) 022474359 $804.00 epResearch Pack 2, (20, 200 and 1,000 µl pipettes) 022474367 804.00 3 3 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 44 Adjustable- and fixed-volume pipettes Eppendorf Research® multichannel pipettes 1 | Instruments | Pipetting | Dispensing Description Long series in columns, rows and plates—that is the area in which the multichannel pipette is becoming increasingly popular. And it is especially for this environment that we developed the Research multichannel pipette, which can be operated easily with one hand. This high-performance 8- or 12-channel pipette offers an effective, precise and rapid way to process long series for an entire spectrum of applications. Reagent reservoir supplied with multichannel pipettes. Each Eppendorf Research multichannel pipette contains a service kit and an epT.I.P.S. Box with the appropriate volume tip. Ordering information: Eppendorf Research multichannel pipettes1 Volume range Description Volume increment Volume Inaccuracy2 Imprecision2 Catalog No. List USD ±8.0% ±4.0% ±2.0% ≤5.0% ≤2.0% ≤1.0% 022452002 022452061 $754.00 874.00 ±3.0% ±1.0% ±0.8% ≤2.0% ≤0.8% ≤0.3% 022452029 022452088 754.00 874.00 ±3.0% ±1.0% ±0.6% ≤1.0% ≤0.5% ≤0.3% 022452045 022452100 754.00 874.00 Grey operating button, for use with 20 μl pipette tips 0.5–10 µl 8-channel 12-channel 0.01 µl 1 µl 5 µl 10 µl Yellow operating button, for use with 200 μl pipette tips 10–100 µl 8-channel 12-channel 0.1 µl 10 µl 50 µl 100 µl Orange operating button, for use with 300 μl pipette tips 30–300 µl 1 2 8-channel 12-channel 0.2 µl 30 µl 150 µl 300 µl U.S. Pat. 5,531,131 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. Ordering information: Accessories Description Catalog No. Reagent Reservoir, autoclavable reservoir for reagent takeup with multichannel pipette, filling volume 50–60 ml, 1 set = 10 pcs. 022265806 List USD $ 39.00 Pipette Carousel Stand, for 6 pipettes 022444905 127.00 Replacement manifolds For 0.5–10 µl 8-channel pipette 022453882 596.00 For 0.5–10 µl 12-channel pipette 022453947 792.00 For 10–100 µl 8-channel pipette 022453904 596.00 For 10–100 µl 12-channel pipette 022453963 792.00 For 30–300 µl 8-channel pipette 022453921 596.00 For 30–300 µl 12-channel pipette 022453980 792.00 Pipette tips information begins on page 53. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 45 Description The patented1 Reference pipette is synonymous with pipetting accuracy, a smooth stroke, a compact and robust design and superior operating comfort. One multifunctional button operates measuring stroke and blow-out as well as ejects the tip. There are 8 adjustable-volume models to cover the range from 0.1 µl to 2,500 µl. Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Ordering information: Reference fixed-volume pipettes Volume Inaccuracy2 Imprecision2 Catalog No. List USD Grey operating button, for use with 20 μl pipette tips or GELoader tip 1 µl ±2.5% ≤1.8% 2 µl ±2.0% ≤1.2% 5 µl ±1.5% ≤0.8% 10 µl ±1.0% ≤0.5% Yellow operating button, for use with 200 μl pipette tips 022470400 022470451 022470507 022470558 $290.00 290.00 290.00 290.00 10 µl ±1.0% ≤0.5% 15 µl ±1.0% ≤0.5% 20 µl ±0.8% ≤0.3% 25 µl ±0.8% ≤0.3% 30 µl ±0.8% ≤0.3% 40 µl ±0.8% ≤0.3% 50 µl ±0.7% ≤0.3% 75 µl ±0.7% ≤0.3% 80 µl ±0.7% ≤0.3% 90 µl ±0.7% ≤0.3% 100 µl ±0.6% ≤0.2% 125 µl ±0.7% ≤0.2% 150 µl ±0.7% ≤0.2% Blue operating button, for use with 1,000 μl pipette tips 022470604 022470655 022470752 022470809 022470850 022470906 022470957 022471007 022471058 022471104 022471155 022471171 022471201 290.00 312.00 290.00 290.00 312.00 312.00 290.00 312.00 312.00 312.00 290.00 290.00 312.00 200 µl 250 µl 300 µl 400 µl 450 µl 500 µl 750 µl 800 µl 900 µl 1,000 µl 022471252 022471309 022471350 022471406 022471431 022471457 022471503 022471554 022471601 022471651 290.00 290.00 290.00 312.00 312.00 290.00 312.00 312.00 312.00 290.00 022471708 022471759 022471805 290.00 290.00 290.00 ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ±0.6% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% ≤0.2% Red operating button, for use with 2,500 μl pipette tips 1,500 µl 2,000 µl 2,500 µl 1 2 ±0.6% ±0.6% ±0.6% ≤0.2% ≤0.2% ≤0.2% U.S. Pat. 5,511,433 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. Liquid: Bidistilled water, degassed Temperature: 20 °C–25 °C, constant to ±0.5 °C No. of measurements: 10, in accordance with EN ISO 8655 using original Eppendorf pipette tips In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Reference fixed-volume and adjustable-volume pipettes 46 Adjustable- and fixed-volume pipettes 1 | Instruments | Pipetting | Dispensing Reference adjustable-volume pipettes All Reference1 pipettes can be readjusted and calibrated for use with liquids that have a different density than water. Product features Fully autoclavable ‡‡ TÜV award: user friendly One-button operation for and user-tested aspiration, dispensing and ‡‡ Single-handed operation tip ejection ‡‡ Four-digit display ‡‡ Volume setting ‡‡ 3-year warranty automatically locks ‡‡ 100% recyclable packaging materials ‡‡ Chemically resistant ‡‡ ‡‡ Each pipette contains a service kit and an epT.I.P.S. Box with the appropriate tips. Ordering information: Reference adjustable-volume pipettes Volume range Volume increment Volume Inaccuracy2 Imprecision2 Catalog No. List USD ≤6.0% ≤1.5% ≤0.7% 022470001 $382.00 1 µl ±2.5% 5 µl ±1.5% 10 µl ±1.0% 2–20 µl 0.02 µl 2 µl ±3.0% 10 µl ±1.0% 20 µl ±0.8% Yellow operating button, for use with 200 μl and 300 μl pipette tips ≤1.8% ≤0.8% ≤0.4% ≤2.0% ≤0.5% ≤0.3% 022470051 382.00 022470108 382.00 2–20 µl 2 µl ±5.0% 10 µl ±1.2% 20 µl ±1.0% 10–100 µl 0.1 µl 10 µl ±3.0% 50 µl ±1.0% 100 µl ±0.8% 50–200 µl 0.2 µl 50 µl ±1.0% 100 µl ±0.9% 200 µl ±0.6% Blue operating button, for use with 1,000 μl pipette tips ≤1.5% ≤0.6% ≤0.3% ≤0.7% ≤0.3% ≤0.15% ≤0.3% ≤0.3% ≤0.2% 022470159 382.00 022470205 382.00 022470256 382.00 100–1,000 µl 1 µl 100 µl ±3.0% 500 µl ±1.0% 1,000 µl ±0.6% Red operating button, for use with 2,500 μl pipette tips ≤0.3% ≤0.2% ≤0.2% 022470302 382.00 500–2,500 µl 2 µl ≤0.3% ≤0.2% ≤0.2% 022470353 382.00 Dark grey operating button, for use with 10 μl pipette tips 0.1–2.5 µl 0.002 µl 0.25 µl 1.25 µl 2.5 µl Grey operating button, for use with 20 μl pipette tips 0.5–10 µl 0.01 µl ±12.0% ±2.5% ±1.4% 0.02 µl 500 µl 1,000 µl 2,500 µl ±1.5% ±0.8% ±0.6% Liquid: Bidistilled water, degassed Temperature: 20°C–25°C, constant to ±0.5 °C No. of measurements: 10, in accordance with EN ISO 8655 using original Eppendorf® pipette tips 1 U.S. Pat. 5,511,433 2 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf pipette tips are used. Ordering information: Accessories Description Catalog No. List USD Pipette Carousel Stand, for six pipettes 022444905 $127.00 Pipette Holder, for wall mounting 022444913 29.00 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Adjustable- and fixed-volume pipettes 47 1 | Instruments | Pipetting | Dispensing epReference3 Pack epReference3 Packs include: ‡‡ Option 1: 3 Eppendorf Reference PhysioCare Concept® pipettes (0.5–10 µl, 10–100 µl, 100–1,000 µl); 3 refillable epT.I.P.S. boxes with 96 tips each; 3 Pipette Holders; SOP and Eppendorf pipette pen! ‡‡ Option 2: 3 Eppendorf Reference PhysioCare Concept pipettes (2–20 µl, 50–200 µl, 100–1,000 µl); 3 refillable epT.I.P.S. boxes with 96 tips each; 3 Pipette Holders; SOP and Eppendorf pipette pen! Description Comfortable, high precision pipetting is the result of perfect teamwork between the pipette and tip. Why not rely upon the unbeatable team of Eppendorf Reference pipettes and epT.I.P.S. pipette tips? Now conveniently available as epReference3 Pack at a special price that’s too good to miss! Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Ordering information Description Catalog No. epReference3 Pack 1, (10, 100 and 1,000 µl pipettes) 022470388 $863.00 epReference Pack 2, (20, 200 and 1,000 µl pipettes) 022470396 863.00 3 List USD In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 48 Electronic pipettes 1 | Instruments | Pipetting | Dispensing Eppendorf Research® pro electronic pipettes 20–300 µl single-channel Research pro 20–300 µl multichannel Research pro Product features ive single-channel models available from 0.5 μl to 5 ml F 8- and 12-channel models available from 0.5 μl to 1,200 μl ‡‡ Volumes can be easily changed in pipetting and dispensing modes ‡‡ Intuitive, easy-to-learn operation ‡‡ Rapid volume selection via predefinable “FIX” volumes ‡‡ Low-force, patented tip ejection for flexible handling ‡‡ Removable, autoclavable and maintenance-friendly lower part ‡‡ Independent adjustment of aspiration and dispensing speeds ‡‡ User-adjustable tip-ejector button ‡‡ Motorized piston control increases reproducibility and decreases repetitive stress ‡‡ High-capacity NiMH battery (at least 3,700 full strokes at high speed with single-channel pipettes) reduces downtime ‡‡ High precision with no reduction in speed ‡‡ Capable of storing up to five commonly used pipetting programs ‡‡ All functions are intuitively executed in a similar manner as per mechanical pipettes ‡‡ 2-year warranty ‡‡ ‡‡ Application ‡‡ Electronic pipetting and dispensing (single-channel and multichannel) Description The Research pro* electronic pipette is ideal for all liquid handling applications. Its easy-to-follow display, ergonomically placed operating buttons and various dispensing techniques, such as pipetting, dispensing, reverse pipetting and diluting, make this a laboratory favorite. Simple functions of the Research pro (pipetting and dispensing, etc.) are intuitively executed in the same manner as a manual pipette. All of the single-channel pipette functions are performed identically in the multichannel version, which means that both models can be used without any additional training. * US Patents 6,499,365/6,778,917 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Electronic pipettes 49 Research pro display Single-channel Research pro Research pro with charging adapter Sliding tip-ejector button is ideal for leftand right-handed users The following functions of the Eppendorf Research pro are available by simply pressing the Select rocker switch: Pipetting (PIP) Standard aspiration and dispensing step with the following optional functions: ‡‡ Blow (separation of stroke and blow-out) ‡‡ Rinse (rinsing and mixing) ‡‡ Reverse pipetting (aspiration with blow-out volume) ‡‡ Manual pipetting (aspiration and dispensing for as long as the key is held down) In addition, the pipette can store up to five programs containing specified pipetting and dispensing sequences, which makes it adaptable to a variety of requirements and also makes everyday repetitive pipetting much easier. More information at www.research-pro.info For pipette service and maintenance information, see page 30; pipette tips information begins on page 53. Dispensing (DIS) Single aspiration, dispensing a maximum of 20 equal partial volumes. Fixed-volume pipetting (FIX) Rapid volume setting from five programmable presettings. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Eppendorf Research® pro electronic pipettes 50 Electronic pipettes 1 | Instruments | Pipetting | Dispensing Eppendorf Research® pro electronic pipettes Ordering information: Eppendorf Research pro electronic pipettes1 Model Volume range Volume increment Volume Inaccuracy2 Imprecision2 Catalog No. List USD Single-channel pipette Grey operating button, for use with 20 μl pipette tips with charging adapter 0.5–10 µl 0.01 µl without charging adapter3 1 µl ±2.5% ≤1.8% 022461303 $ 659.00 5 µl ±1.5% ≤0.8% 022461354 633.00 10 µl ±1.0% ≤0.4% Yellow operating button, for use with 200 μl pipette tips with charging adapter 5–100 µl 0.1 µl without charging adapter3 10 µl ±2.0% ≤1.0% 022461311 659.00 50 µl ±1.0% ≤0.3% 022461362 633.00 100 µl ±0.8% ≤0.2% Yellow operating button, for use with 300 μl pipette tips with charging adapter 20–300 µl 0.5 µl without charging adapter3 30 µl ±2.5% ≤0.7% 022461320 659.00 150 µl ±1.0% ≤0.3% 022461371 633.00 300 µl ±0.6% ≤0.2% Blue operating button, for use with 1,000 μl pipette tips with charging adapter 50–1,000 µl 1 µl without charging adapter3 100 µl ±3.0% ≤0.6% 022461338 659.00 500 µl ±1.0% ≤0.2% 022461389 633.00 1,000 µl ±0.6% ≤0.2% Violet operating button, for use with 5,000 μl pipette tips with charging adapter 100–5,000 µl 10 µl without charging adapter3 500 µl ±3.0% ≤0.6% 022461346 659.00 2,500 µl ±1.2% ≤0.25% 022461397 633.00 5,000 µl ±0.6% ≤0.15% 1 µl ±5.0% ≤3.0% 022461401 1,172.00 Multichannel pipette Grey operating button, for use with 20 μl pipette tips with charging adapter 8-channel 0.5–10 µl 0.01 µl w/o charging adapter 8-channel with charging adapter 3 5 µl ±3.0% ≤1.5% 022461443 1,121.00 12-channel 10 µl ±2.0% ≤0.8% 022461486 1,419.00 022461516 1,386.00 1,172.00 w/o charging adapter 12-channel3 Yellow operating button, for use with 200 μl pipette tips with charging adapter 8-channel 5–100 µl 0.1 µl w/o charging adapter 8-channel3 with charging adapter 12-channel 10 µl ±2.0% ≤2.0% 022461419 50 µl ±1.0% ≤0.8% 022461451 1,121.00 100 µl ±0.8% ≤0.25% 022461494 1,419.00 022461524 1,386.00 w/o charging adapter 12-channel3 Orange operating button, for use with 300 μl pipette tips with charging adapter 30 µl ±2.5% ≤1.0% 022461427 1,172.00 w/o charging adapter 8-channel3 8-channel 20–300 µl 0.5 µl 150 µl ±1.0% ≤0.5% 022461460 1,121.00 with charging adapter 300 µl ±0.6% ≤0.25% 022461508 1,419.00 022461532 1,386.00 12-channel w/o charging adapter 12-channel 3 Green operating button, for use with 1,250 μl pipette tips with charging adapter 8-channel w/o charging adapter 8-channel 3 1 50–1,200 µl 5 µl 120 µl ±6.0% ≤0.9% 022461435 1,172.00 600 µl ±2.7% ≤0.4% 022461478 1,121.00 1,200 µl ±1.2% ≤0.3% U.S. Pats. 6,499,365/6,778,917 The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. A single- or four-place charging stand must be purchased with the pipette to recharge the unit. 2 3 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipette stand 51 1 | Instruments | Pipetting | Dispensing Eppendorf Research® pro electronic pipette accessories Charging stand for single-channel and multichannel pipettes Application of Research pro Ordering information Description Catalog No. Charging stand (including charging adapter) For one pipette 022461541 List USD $217.00 Charging stand (including charging adapter) For four pipettes 022461559 639.00 Pipette tips information begins on page 53. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 52 Pipette accessories 1 | Instruments | Pipetting | Dispensing Eppendorf® pipette accessories Pipette holders for wall mounting Carousel Stand Ordering information Product features arousel stand for six pipettes—stores all Research and C Research plus single-channel and multichannel pipettes1 in a minimal space ‡‡ Suitable for use with all Reference2 pipettes without an adapter ‡‡ With an adapter, also suitable for use with Repeater®, Repeater Plus3 and Maxipettor® pipettes ‡‡ Description Enjoy an organized workplace with our rotating pipette stand. Pipettes attach to the stand by the handle, not the nose cone, which eliminates potential cross-contamination from the stand to the pipettes. In addition to the Eppendorf Research® and Reference pipettes, the stand can also hold all older models of Eppendorf pipettes. Description Catalog No. List USD Pipette Carousel Stand, for six pipettes 022444905 $127.00 Pipette holder, for wall mounting 022444913 29.00 Repeater Plus/Maxipettor adapter, for pipette carousel stand 022260588 18.00 Repeater Plus/Maxipettor adapter, for wall mounting 022260596 29.00 1 U.S. Pats. 5,531,131/6,119,435 2 U.S. Pat. 5,511,433 3 U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,729 For pipette service and maintenance information, see page 30; pipette tips information begins on page 53. Also available: a pipette holder that attaches to the wall, which keeps the pipette within reach, even when you have limited bench space. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipette tips 53 A pipette is only as good as its tip Only a perfectly manufactured pipette tip can ensure that the precision of a modern pipette is maximized. Our epT.I.P.S.— Eppendorf Totally Integrated Pipetting System—work in perfect combination with our pipettes, resulting in reduced-force tip attachment and ejection with complete sealing. Additionally, the universal nose cone design allows the tips to be used with pipettes from other manufacturers. Production without compromise Having our own production facility means that each one of our epT.I.P.S. is manufactured to the highest quality standards. Through our selection of the best plastic materials and ISO 9001-certified production processes, our tips maintain ideal wetting properties and boast high transparency and certified purity levels. Every tip is manufactured to match specifically to its respective Eppendorf® pipette(s), thus ensuring the maximum precision and reliability you have come to expect and rely on. The Eppendorf pipette and tip system satisfies all of the requirements of EN ISO 8655 standards. Product features of epT.I.P.S. rop-contour design gives all Eppendorf tips a D uniform look ‡‡ Soft, flexible tip cone ensures better pipette tip sealing ‡‡ Ergonomically optimized cone geometry ensures perfect fit on Eppendorf pipettes ‡‡ Universal fit—can be used with pipettes from other manufacturers ‡‡ Fine graduations make it easy to visually check the volume pipetted ‡‡ Available in five purity levels: Eppendorf Quality, Sterile, PCR clean, PCR clean sterile and free of pyrogens, and Eppendorf Biopur® ‡‡ ew tip design for better access to a wider variety N of tubes and wells ‡‡ Finely tapered and extended for working with small volumes ‡‡ Can you see the quality of your tips? Eppendorf tip Brand A Brand B Brand C Brand D Brand E In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing TI PS Eppendorf® Totally I ntegrated P ipetting S ystem 54 Pipette tips TI PS 1 | Consumables | Pipetting | Dispensing Handling the reload system—Contamination Free! Lift Lift The transparent blue protective lid for epT.I.P.S. Reloads allows individual trays to be lifted off of the respective stack (left) or dual tray (right) without contamination. Transfer Engage An audible “click” indicates that the tray is fully engaged. The blue lid can be used to reseal the epT.I.P.S. Reloads to protect the tips from contamination. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipette tips 55 Eppendorf Quality Eppendorf Sterile Eppendorf PCR clean Eppendorf PCR clean, sterile and free of pyrogens Eppendorf Biopur® Continuous quality control for the following quality criteria: Function, tightness, precision ‡ ‡ ‡ ‡ ‡ Defined flow dynamics ‡ ‡ ‡ ‡ ‡ Minimal water retention ‡ ‡ ‡ ‡ ‡ High chemical resistance ‡ ‡ ‡ ‡ ‡ High thermal stability ‡ ‡ ‡ ‡ ‡ Precisely molded ‡ ‡ ‡ ‡ ‡ ‡ ‡ ‡ DNase-free ‡ ‡ RNase-free ‡ ‡ Free of PCR inhibitors ‡ ‡ Batch testing (certified) for the following purity criteria: 1 Free of human DNA Free of bacteria DNA (E. coli) ‡ ATP-free ‡ ‡ Pyrogen-free (endotoxin-free) Sterile (Ph.Eur./USP) ‡ ‡ ‡ ‡ ‡ Eppendorf products Tips epT.I.P.S. Standard (Bulk) ‡ epT.I.P.S. Box ‡ epT.I.P.S. Reloads ‡ epT.I.P.S. Starter Set ‡ epT.I.P.S. Racks ‡ epT.I.P.S. Singles (individually packaged) ‡ ‡ ‡ ‡ ‡ ‡ ep Dualfilter T.I.P.S. ‡ Combitips Plus tips ‡ epT.I.P.S. LoRetention ‡ epT.I.P.S. Motion ‡ epT.I.P.S. Motion filtertips ‡2 ‡ ‡ ‡ ‡ ‡ ‡ Tubes Safe-Lock tubes ‡ ‡ Flex-Tube ‡ ‡ ® Protein LoBind tubes ‡ DNA LoBind tubes ‡ PCR Tubes (PCR and qPCR) ‡ Cap Strips (PCR and qPCR) ‡ ‡2 Plates and accessories Deepwell Plates 1 2 ‡ ‡ Protein LoBind Deepwell Plates ‡ DNA LoBind Deepwell Plates ‡ twin.tec PCR Plates (PCR and qPCR) ‡ Self-adhesive sealing materials ‡ Heat sealing materials ‡ Capping Aid ‡ in situ Frame ‡ Batch-specific certificates can be downloaded from www.eppendorf.com. Individually packaged. For more information on Eppendorf purity levels, see pages 301 to 305, or visit www.eppendorf.com/purity. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing Eppendorf purity levels for tips, Combitips®, tubes and plates 56 Pipette tips 1 | Consumables | Pipetting | Dispensing epT.I.P.S. LoRetention Simple differentiation of racks and reloads through a new, clear lid with a white seal. When open, the writing “LoRetention” can be seen from the inside. The reusable seal also makes it possible to label existing epT.I.P.S. boxes. 2 1 The “Pearl Effect”—a comparison of the amount of residual liquid when pipetting solutions that contain detergents: Maximum recovery with epT.I.P.S. LoRetention Significant loss of sample using standard tips Product features Ultrahomogeneous surface for maximum reproducibility ‡‡ Ultrahydrophobic surface for minimum loss of sample ‡‡ Air bubbles and foaming are significantly reduced during pipetting ‡‡ Autoclavable, high chemical resistance ‡‡ 5 -year shelf life ‡‡ Available as ep Dualfilter T.I.P.S., as well as in PCR clean and Eppendorf Quality ‡‡ Maximum results The ultrahydrophobic, extremely homogenous surface of the epT.I.P.S. LoRetention is achieved through an innovative treatment at the molecular level—the Pearl Effect Technology. The tips are uncoated and free from additives, thus nothing can bleed into the sample. Liquids roll off completely, so that almost no sample remains in the tip. In comparison, the adhesive layer of liquid in standard tips retains considerably more sample material. Reduce costs, increase reproducibility Particularly with sensitive PCR and real-time PCR applications, low-retention surfaces can increase reproducibility and notably reduce the loss of expensive reagents—depending on the reagent solution by up to 5%! Various applications epT.I.P.S. LoRetention are ideally suited for all applications with samples that contain detergents: ‡ PCR and real-time PCR preparation of enzyme solutions and master mixes ‡ Isolation, purification and denaturation of proteins ‡ Restriction digestions and ligations ‡ Pipetting DNA ladders during gel electrophoresis epT.I.P.S. LoRetention perform considerably better in terms of precision and sample recovery than low-retention tips from other manufacturers. This unique material property guarantees maximum sample recovery, improved reproducibility and higher sensitivity. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipette tips 57 Quality and purity Eppendorf style It goes without saying that the new epT.I.P.S. LoRetention have all the valued properties of the proven Eppendorf epT.I.P.S. They are produced in high-precision manufacturing processes, can be autoclaved, are extremely resistant to chemicals, and are available in the PCR clean (free of human DNA, DNase, RNase, and PCR inhibitors) purity grade, as well as in Eppendorf Quality. For maximum contamination protection, epT.I.P.S. LoRetention are also available as ep Dualfilter T.I.P.S. ep T.I.P.S. LoRetention 0.1% Triton X-100 6.0 6.0 PCR-Mastermix Low-Retention Competitor M 5.0 5.0 Residual liquid [µl] Residual liquid [µl] Low-Retention Competitor A 4.0 3.0 4.0 3.0 2.0 2.0 1.0 1.0 0.0 0.0 epT.I.P.S LoRetention Competitor A Low-Retention Competitor M Low-Retention Competitor S Low-Retention Untreated Fig. 1. Comparison of residual liquid from epT.I.P.S. LoRetention and low-retention tips of other manufacturers. 200 µl tips were used to pipette a commercial PCR master mix and the detergent 0.1% Triton X-100. Once the liquid had been dispensed, the residual liquid in the tips was determined. The measurements were repeated several times and the standard deviation determined. epT.I.P.S. LoRetention resulted in the lowest residual liquid, as well as the smallest standard deviation. Acetonitrile Acetone Isopropanol Autoclaved Fig. 2. Comparison of resistance of the low-retention property when subjected to solvents or autoclaving. 200 µl epT.I.P.S. LoRetention and low-retention tips of other manufacturers were autoclaved or treated with the solvent specified. An enzymatic buffer containing detergents was then pipetted and the residual moisture was determined. epT.I.P.S. LoRetention resulted in reproducible low levels of residual liquid with minimum standard deviation. Low-retention tips from other manufacturers are less resistant to chemicals or autoclaving: this resulted in an increase in residual liquid and standard deviation. Ordering information Description Catalog No. List USD epT.I.P.S. LoRetention Dualfilter, Racks, PCR clean/sterile/pyrogen-free, 10 × 96 tips 0.1–10 µl Short 022493000 Description Catalog No. List USD epT.I.P.S LoRetention, Reloads, Eppendorf Quality, 10 × 96 tips $142.00 0.1–10 µl Short 022493018 $86.00 0.5–20 µl Long 022493002 142.00 0.5–20 µl Long 022493020 86.00 2–100 µl 022493006 132.00 2–200 µl 022493022 68.00 20–300 µl 022493004 142.00 50–1,000 µl 022493024 75.00 142.00 epT.I.P.S LoRetention, Racks, PCR clean, 10 × 96 tips 50–1,000 µl 022493008 epT.I.P.S LoRetention, Reloads, PCR clean, 10 × 96 tips 0.1–10 µl Short 022493026 116.00 0.1–10 µl Short 022493010 98.00 0.5–20 µl Long 022493028 116.00 0.5–20 µl Long 022493012 98.00 2–200 µl 022493030 92.00 50–1,000 µl 022493032 99.00 2–200 µl 022493014 80.00 50–1,000 µl 022493016 86.00 For more information on Eppendorf purity levels, see pages 301 to 305, or visit www.eppendorf.com/purity. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing epT.I.P.S. LoRetention 58 Pipette tips epT.I.P.S. Bulk standard riginal, high-quality Eppendorf pipette tips at an O attractive price ‡‡ Packaged in resealable bags ‡‡ Available in sizes from 10 μl to 10 ml 1 | Consumables | Pipetting | Dispensing ‡‡ Ordering information Description Catalog No. epT.I.P.S. Bulk, 2 bags of 500 tips = 1,000 tips Eppendorf Quality 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm, 5 bags of 100 tips = 500 tips 100–5,000 µl, 120 mm, 5 bags of 100 tips = 500 tips 1–10 ml, 165 mm, 5 bags of 100 tips = 500 tips 1–10 ml L, 243 mm, 5 bags of 100 tips = 500 tips 022492004 022492012 022492021 022492039 022492047 022492055 022492063 022492071 022492080 022492098 022492101 List USD $62.00 62.00 62.00 50.00 50.00 53.00 53.00 62.00 86.00 48.00 54.00 ep Dualfilter T.I.P.S. ll filter tips are Sterile (to USP, DAB, Ph.Eur. standards) A and PCR clean (free of human DNA, DNase, RNase, PCR inhibitors and pyrogens) ‡‡ Continuous control of each batch by an independent lab – Batch-related certificates available on request ‡‡ Pipette tips have an integrated dual pore filter made of hydrophobic polyethylene and do not contain “self-sealing” additives ‡‡ Sample can be easily recovered, even if the filter becomes wet as a result of incorrect pipetting ‡‡ Packaged in racks of 96 tips ‡‡ Ordering information Description Catalog No. epT.I.P.S. Dualfilter, 10 racks of 96 tips = 960 tips, PCR clean and sterile 0.1–10 µl S, 34 mm 0.1–10 µl M, 40 mm 0.5–20 µl L, 46 mm 2–20 µl, 55 mm 2–100 µl, 55 mm 2–200 µl, 55 mm 20–300 µl, 55 mm 50–1,000 µl, 71 mm 100–5,000 µl, 120 mm, 5 racks of 24 tips = 120 tips 1–10 ml L, 243 mm, 5 racks of 24 tips = 120 tips 022491202 022491211 022491229 022491270 022491237 022491296 022491245 022491253 022491261 022491288 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. List USD $134.00 134.00 134.00 134.00 124.00 124.00 124.00 134.00 74.00 62.00 Pipette tips epT.I.P.S. Box 59 User friendly: ‡‡ Contamination-free transfer of trays to the working box ‡‡ System optimized for use with multichannel pipettes ‡‡ Color-coded trays for simple identification of tips and matching Eppendorf® pipette ‡‡ Tips can be attached to the pipette from the refill trays ‡‡ Refill trays are entirely autoclavable for future use ‡‡ Space-saving packaging Ordering information Description Catalog No. epT.I.P.S. Box, 1 reusable box includes 96 tips, Eppendorf Quality 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm, includes 48 tips 100–5,000 µl, 120 mm, includes 24 tips 022491300 022491318 022491326 022491334 022491342 022491351 022491369 022491377 022491385 epT.I.P.S. Set List USD $32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00 32.00 set Starter kits consist of the epT.I.P.S. Box and 5 refill trays Ordering information Description Catalog No. epT.I.P.S. Starter Set, 1 reusable box includes 5 trays of 96 tips = 480 tips 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm, 5 trays of 48 tips = 240 tips 022491407 022491415 022491423 022491431 022491440 022491458 022491466 022491474 List USD $62.00 62.00 62.00 50.00 50.00 53.00 53.00 63.00 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing box 60 Pipette tips 1 | Consumables | Pipetting | Dispensing epT.I.P.S. Reloads reloads Environmentally friendly: All components are 100% recyclable ‡‡ Reduced waste as compared with disposable racks ‡‡ The refill system, depending on tip size, is packaged as either dual-sided or in stack form ‡‡ Reloads are available in two purity levels: Eppendorf Quality and PCR clean ‡‡ Ordering information Description Catalog No. epT.I.P.S. Reloads, 10 trays of 96 tips = 960 tips 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm, 10 trays of 48 tips = 480 tips Eppendorf Quality 022491504 022491512 022491521 022491539 022491547 022491555 022491563 022491571 List USD Catalog No. $78.00 78.00 78.00 60.00 60.00 67.00 67.00 67.00 PCR Clean 022491709 022491717 022491725 022491733 022491741 022491750 022491768 022491776 epT.I.P.S. Racks List USD $90.00 90.00 90.00 72.00 72.00 78.00 78.00 78.00 racks onvenient, ready-to-use pipette tip racks are available in C four purity levels: Eppendorf Quality, Sterile, PCR clean and Eppendorf Biopur® ‡‡ Packaged in racks of 96, 48 or 24 tips, depending on tip volume ‡‡ Ordering information Description Cat. No. epT.I.P.S. Racks, 10 racks of 96 tips = 960 tips 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm, 5 racks of 48 tips = 240 tips 100–5,000 µl, 120 mm, 5 racks of 24 tips = 120 tips 1–10 ml, 165 mm, 5 racks of 24 tips = 120 tips Eppendorf Quality 022491903 022491911 022491920 022491938 022491946 022491954 022491962 022491971 List USD Cat. No. $86.00 86.00 86.00 67.00 67.00 73.00 73.00 37.00 022491989 43.00 022491997 61.00 List USD Cat. No. Sterile 022492250 $108.00 022492276 022492284 022492292 022492306 84.00 84.00 91.00 91.00 022492314 54.00 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. List USD Cat. No. PCR Clean 022491806 022491814 022491822 022491831 022491849 022491857 022491865 022491873 $108.00 108.00 108.00 84.00 84.00 91.00 91.00 47.00 022491881 54.00 List USD Biopur 022491067 $86.00 022491083 022491091 022491105 022491113 022491121 80.00 80.00 85.00 85.00 85.00 022491164 74.00 Pipette tips epT.I.P.S. Singles 61 singles Individually wrapped pipette tips available in two purity levels Batch number and expiration date printed on each tip package ‡‡ Continuous quality control of each batch by an independent laboratory – Batch-specific purity certificates available upon request, or visit www.eppendorf.com ‡‡ Ordering information Description Catalog No. Sterile (1,000 tips) epT.I.P.S. Singles, individually wrapped 0.1–10 µl, 34 mm 0.1–20 µl, 40 mm 0.5–20 µl L, 46 mm 2–200 µl, 53 mm 20–300 µl, 53 mm 50–1,000 µl, 71 mm 50–1,250 µl, 76 mm 500–2,500 µl, 115 mm 100–5,000 µl, 120 mm 1–10 ml, 165 mm 1–10 ml L, 243 mm List USD Catalog No. List USD Biopur (100 tips) 022491130 $29.00 022492209 $129.00 022491148 24.00 022492225 129.00 022491156 25.00 TI PS Special tips Volume range/pipette tip GELoader Catalog No. List USD 0.5 µl–20 µl, 62 mm 2 racks of 96 tips = 192 tips 022351656 $121.00 930001007 148.00 Microloader 0.5 µl–20 µl, 100 mm 2 racks of 96 tips = 192 tips Note: All pipette tips meet the Eppendorf Quality purity standard; where applicable, higher purity levels are noted. customized products p. 256 How can you recognize original Eppendorf® pipette tips? A unique feature of our tips is the “ring” of drop-shaped relief elements circling the crown. Additionally, each tip (and tube) is embossed with “Eppendorf” on its rim. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing ‡‡ 62 Pipette tips 1 | Consumables | Pipetting | Dispensing epT.I.P.S.® Elongated Product features ighest protection for pipette and sample with ep H Dualfilter T.I.P.S. L (103 mm in volume variant of 1,250 µl) ‡‡ Available in four purity standards: Eppendorf Quality, PCR clean, PCR clean/sterile/pyrogen-free and Eppendorf Biopur® ‡‡ Safe access to Eppendorf Deepwell Plates or other deep and narrow vessels ‡‡ Highest precision and accuracy when using epT.I.P.S. 50 µl–1,250 µl L pipette tips in system with the Eppendorf Research® pro 8-channel pipette ‡‡ Easy recognition through new “dark green” tray color ‡‡ Description With the new epT.I.P.S. 50 µl–1,250 µl L pipette tip you will always receive best results while pipetting from and into 15 ml conical tubes, high reagent bottles, narrow deep vessels and especially Eppendorf Deepwell Plates. The long and slim design of this new elongated pipette tip gives you free access to your sample with reduced risk of touching the sides of tubes or wells. Cross-contamination during pipetting can be avoided to a minimum. Even smallest volumes in deepwell plates of 96 and 384 wells can be almost completely recovered from the bottom. Compatibility of epT.I.P.S. 1,250 µl L and Eppendorf pipettes Description Model Eppendorf Reference fixed-volume 50–1,000 µl 100–1,000 µl X fixed-volume X adjustable Eppendorf Research pro single-channel 50–1,200 µl* X adjustable Eppendorf Research® 200–1,000 µl X X multichannel* Eppendorf Research plus X* single-channel X *Special standard of precision and accuracy in accordance with EN ISO 8655 is given in combination with the Eppendorf Research pro multichannel pipette 50–1,200 µl. Universal fit is given in combination with pipettes of the above mentioned volumes from other manufacturers. Ordering information Description Purity Catalog No. List USD ep Dualfilter T.I.P.S. , Racks, 5 × 96 = 480 tips ® 50 µl–1,250 µl L PCR clean, sterile and pyrogen-free 022494002 $70.00 epT.I.P.S., Reloads, 10 × 96 = 960 tips 50 µl–1,250 µl L Eppendorf Quality 022494004 70.00 50 µl–1,250 µl L PCR Clean 022494006 80.00 epT.I.P.S., Racks, 5 × 96 = 480 tips 50 µl–1,250 µl L Eppendorf Quality 022494010 38.00 50 µl–1,250 µl L PCR Clean 022494012 47.00 50 µl–1,250 µl L Eppendorf Biopur® 022494014 47.00 epT.I.P.S., Reusable Box, 1 × 96 tips 50 µl–1,250 µl L Eppendorf Quality 022494016 33.00 epT.I.P.S., Standard, 2 bags of 500 = 1,000 tips 50 µl–1,250 µl L Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Eppendorf Quality 022494018 54.00 Positive displacement pipettes and tips 63 Application Contamination-free pipetting in positive-displacement mode ‡‡ Ideal for liquids that are viscous (e.g., glycerol) or have high vapor pressure (e.g., ethanol, acetone) Product features ‡‡ Single-handed tip attachment and ejection Lateral volume locking ‡‡ Ergonomic design ‡‡ UV-resistant and fully autoclavable ‡‡ Maintenance-free ‡‡ 3-year warranty ‡‡ ‡‡ Description Enjoy contamination-free pipetting with the Positive Displacement Pipette, an adjustable pipette for the volume range from 1 μl to 20 μl. With the Positive Displacement Tip, the pipette functions according to the positive-displacement principle; thus, the formation of aerosols—a common cause of contamination—is eliminated. Technical specifications Volume Volume increment Inaccuracy* Imprecision* 2 µl 0.02 µl ±6.0% ≤ 4.0% 3 µl ±5.0% ≤ 3.0% 5 µl ±4.0% ≤ 2.0% 10 µl ±3.0% ≤ 1.5% 20 µl ±2.5% ≤ 0.8% Liquid: Bidistilled water Temperature: 20 °C–25 °C, constant to ±0.5 °C No. of 10, in accordance with EN ISO 8655 using original Eppendorf® pipette tips measurements: * The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf pipette tips are used. Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Ordering information Description Catalog No. BioMaster Positive Displacement Pipette kit, includes 1 Positive Displacement Pipette with 1–20 μl continuous volume selection and 1 box of 96 Positive Displacement Tips 022440501 List USD $433.00 Positive Displacement Tips Product features ‡‡ ‡‡ eady-for-use piston integrated in tip R 96 positive displacement tips per rack Ordering information Description Catalog No. Positive Displacement Tips, includes ready-for-use piston, 5 racks of 96 tips 022354159 List USD $248.00 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing BioMaster—Positive Displacement Pipette 64 Positive displacement pipettes 1 | Instruments | Pipetting | Dispensing Maxipettor® with Maxitip P and Maxitip S 1–Maxitip P 2–Maxitip S Kit 3–Maxitip S 4–Maxitip G 1 Product features of Maxipettor 2 3 4 Product features of Maxitips djustable pipette for 1 ml to 10 ml A Control button for volume selection ‡‡ Digital volume display ‡‡ Maintenance-free ‡‡ Exchangeable Maxitips axitip P for aspirating 1 ml to 10 ml from beakers using M a positive displacement technique ‡‡ Maxitip S for aspirating 2.5 ml to 10 ml from tall, narrow-neck vessels using an air-displacement technique ‡‡ No carryover as Maxitips are exchanged ‡‡ Valve for Maxitip ensures drip-free dispensing of liquids with a high vapor pressure ‡‡ Maxitip G is graduated for dispensing accurate volumes of nonaqueous liquids ‡‡ ‡‡ ‡‡ Description The Maxipettor is an incrementally adjustable pipette for precise pipetting of volumes up to 10 ml (in 10 μl increments). Even liquids with a high vapor pressure or viscosity can be easily pipetted. Ordering information Description Catalog No. Maxipettor, with 1 ml–10 ml continuous volume adjustment 022290002 List USD $688.00 Maxitip P (positive displacement), 022291050 nonsterile, pkg. of 100 186.00 Maxitip P, Eppendorf Biopur, (positive displacement), sterile, individually wrapped, pkg. of 100 022292056 259.00 Maxitip S Kit (air displacement), includes 10 barrels with pistons, 100 Maxitip S, 2 Maxitip G, 10 Valves 022291301 175.00 Barrels with Pistons, for Maxitip S, pkg. of 30 022291408 134.00 Maxitip S (air displacement), pkg. of 200 022291351 149.00 Valves, for Maxitip S and G, pkg. of 100 022291459 66.00 Maxitip G, pkg. of 5 022291505 78.00 Stand for Maxipettor, holds two units 022260651 67.00 Description The pipette tips for the Maxipettor are tailored to different vessels. For example, the Maxitip P is designed for aspirating 1 ml to 10 ml from beakers, and it pipettes according to the positive displacement principle. The Maxitip S for 2.5 ml to 10 ml forms a system with the Maxipettor, and it is used for aspirating liquids from tall, narrow-neck vessels. Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Technical specifications Nominal volume Inaccuracy* Imprecision* Maxipettor and Maxitip S 2.5 ml 5 ml 10 ml ±1.0% ±0.4% ±0.3% ≤ 0.2% ≤ 0.2% ≤ 0.2% Maxipettor and Maxitip P 1.0 ml 5 ml 10 ml ±0.6% ±0.5% ±0.3% ≤ 0.2% ≤ 0.1% ≤ 0.1% Measuring conditions in accordance with EN ISO 8655: Bidistilled water, 20 °C, n = 10 *The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® pipette tips are used. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Manual and electronic dispensers 65 Selection Guide for Eppendorf® Repeater pipettes Repeater Plus Repeater stream Repeater Xstream manual dispenser electronic dispenser electronic dispenser Catalog No. 022260201 $485.00 $884.00 $1,061.00 Volume settings 20 steps Unlimited Unlimited LCD Illuminated Illuminated Positive displacement principle ‡ ‡ ‡ Automatic Combitips Plus tip recognition ‡ ‡ ‡ Volume range from 1 μl to 50 ml ‡ ‡ ‡ PhysioCare Concept® design ‡ ‡ ‡ Pipetting ‡ ‡ ‡ Dispensing ‡ ‡ ‡ 2-year warranty ‡ ® ‡ ‡ Automatic dispensing with programmable time interval ‡ ‡ Rapid, ergonomic dispensing with motorized piston ‡ ‡ One button tip ejector ‡ ‡ Long life Li-ion battery ‡ ‡ Real-time countdown of dispensings displayed ‡ ‡ Real-time troubleshooting prompts ‡ ‡ Aspiration (pipetting of supernatants) ‡ Titration ‡ Sequential dispensing ‡ The instruments The manual Repeater Plus1 and the electronic Repeater stream/Xstream hand dispensers are ideal for process acceleration and saving time on long-series pipetting: their increased volume selection provides outstanding flexibility and exceptional support for research as well as routine tasks; and their smooth handling reduces the fatigue associated with repetitive work. The consumables Combitips Plus tips2 easily attach to the Repeaters; their built-in sensor recognizes the size of the tip, and the volume appears automatically in the display—eliminating time-consuming volume calculations and incorrect dispensing volumes. The nine sizes of Combitips Plus allow a wider dispensing range of up to 112 different volumes and longer series dispensing (e. g., 100 x 250 μl) without stopping for a refill. Always contamination-free! 2 022460811 (incl. charging adapter) List USD Display LCD 1 022460803 (incl. charging adapter) A wide variety of solutions Combitips Plus tips function according to the positive displacement principle, which ensures that the correct volume is dispensed regardless of the density or viscosity of the liquid. The positive displacement system provides safer dispensing of solutions that contain radioactive and/or toxic substances, as well as solutions that have a high vapor pressure, because no aerosols are formed. Combitips are easily replaced—eject with the push of a button—and dispose after use, always contamination-free. U.S. Patents 5,620,660/5,620,661/5,591,405/5,573,729 U.S. Patents 5,620,660/5,620,661 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Electronic and manual Repeater® Plus and stream/Xstream 66 Manual and electronic dispensers 1 | Instruments | Pipetting | Dispensing Electronic Repeater® stream and Repeater Xstream handheld dispensers Illuminated display for easy viewing. Product features lectronic dispensing with programmable E memory functions ‡‡ Contamination-free dispensing using the positive displacement principle ‡‡ Dispensing from 1 μl to 50 ml ‡‡ Dispensing volumes in long series ‡‡ See page 65 for additional features ‡‡ Description Electronic Repeater stream and Xstream combine easy handling with a broad range of features. When used with Combitips Plus®* tips (pages 68 to 71), the dispenser/tip forms an unbeatable system that is the quintessential lab tool for all applications: Repeater stream is suitable for all standard pipetting and dispensing applications; for added versatility, the Repeater Xstream can also perform titrations, sequential dispensing and aspiration of supernatants. * U.S. Patents 5,620,660/5,620,661 For technical specifications, see page 69. Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Ordering information Description Catalog No. List USD Repeater stream electronic dispenser 022460803 $ 884.00 Repeater Xstream electronic dispenser 022460811 1,061.00 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Manual and electronic dispensers 67 1 | Instruments | Pipetting | Dispensing Manual Repeater® Plus handheld dispenser The rounded, grooved grip of the Repeater Plus means easier dispensing with less stress on the hand and thumb muscles. Product features ispensing volumes in long series D Contamination-free dispensing using the positive displacement principle ‡‡ Ideal for viscous solutions (e.g., glycerol, oil) or liquids with high vapor pressure (e.g., acetone, ethanol) ‡‡ Easy to read: automatic Combitips Plus® recognition with electronic volume display ‡‡ Easy volume setting ‡‡ More flexible with 112 different volumes from 1 µl to 10 ml ‡‡ Up to 100 dispensings per filling for easy processing of long series ‡‡ Versatile, even for “uneven” volumes, e.g., 1 µl, 3 µl or 17 µl ‡‡ Safe, due to easy, contact-free Combitips Plus attachment and ejection ‡‡ 2-year warranty ‡‡ See page 65 for additional features ‡‡ ‡‡ Description Repeater Plus1 is the ideal precision instrument for reducing the time associated with manual, long-series pipetting. Its increased volume selection provides outstanding flexibility and all-around support for research and routine tasks. The electronic display ensures error-free dispensing, and the rounded, grooved grip makes dispensing easier and ergonomic—with less stress on the hand and thumb muscles. 1 Ordering information Description Catalog No. Repeater Plus manual dispenser 022260201 List USD $485.00 Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. U.S. Pats. 5,620,660/5,620,661/5,591,405/5,573,729 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 68 Manual and electronic dispensers 1 | Consumables | Pipetting | Dispensing Combitips Plus® tips and Repeater® Plus, stream and Xstream accessories Product features of Combitips Plus Ideal system component for all Repeater, Repeater Plus and Repeater stream/Xstream (electronic) ‡‡ 20 different dispensing volumes for each Combitip size (Repeater Plus) ‡‡ Several hundred different volumes for each Combitip size (Repeater stream/Xstream) ‡‡ Higher throughput with up to 100 dispensings per filling ‡‡ Reliable results ensured by positive displacement system ‡‡ Available in Eppendorf Quality, Sterile and Eppendorf Biopur purity levels ‡‡ Available in nine different sizes from 0.1 ml to 50 ml ‡‡ Certified trace metal release ‡‡ Wall mount Pipette stand Accessories As a safeguard against potential contamination, our carousel stand does not hold the pipette at the bottom—like most other stands do. Eppendorf Repeater, Repeater Plus and Maxipettor® pipettes easily fit on the carousel with an adapter. To store the electronic Repeater stream/Xstream, a charging stand is available (sold separately). Is your bench space at a premium? We also offer the practical wall-mounted pipette bracket—it keeps your Repeater Plus off the bench but still within reach. Tip purity is ensured In addition to standard purity, Combitips Plus tips are available in Sterile and Eppendorf Biopur®. Eppendorf Biopur (page 305), signifying outstanding biological purity, guarantees that these tips are sterile as well as pyrogen-, RNase-, DNase and ATPfree. They meet the most stringent demands of the medical, pharmaceutical and food industries, PCR applications and molecular biology/cell technology labs. This purity is ensured through an automated manufacturing process, which safeguards the product against contamination with biological substances. Individual lot certificates are available at www.eppendorf.com. Charging stand Ordering information Description Catalog No. Charging stand, for Repeater stream/Xstream, holds one unit 022460897 $194.00 Stand for Repeater pipettes, holds two units 022260651 67.00 Repeater Plus adapter, for new pipette carousel stand 022260588 18.00 Repeater Plus Wall Mount 022260596 29.00 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. List USD Manual and electronic dispensers 69 Technical specifications Combitips Plus Volume min./max. Increment/ step size Max. dispensing Volume volume Inaccuracy1 Imprecision1 For Repeater Plus2 and Combitips Plus3 system 0.1 ml 1 µl 1 µl 20 µl 20 µl 2 µl 20 µl ±1.6% ±1.0% ≤3.0% ≤2.0% 0.2 ml 2 µl 2 µl 40 µl 40 µl 4 µl 40 µl ±1.3% ±0.8% ≤2.0% ≤1.5% 0.5 ml 5 µl 5 µl 100 µl 100 µl 10 µl 100 µl ±0.9% ±0.8% ≤1.5% ≤0.6% 1 ml 10 µl 10 µl 200 µl 200 µl 20 µl 200 µl ±0.9% ±0.6% ≤0.9% ≤0.4% 2.5 ml 25 µl 25 µl 500 µl 500 µl 50 µl 500 µl ±0.8% ±0.5% ≤0.8% ≤0.3% 5 ml 50 µl 50 µl 1,000 µl 1,000 µl 100 µl 1,000 µl ±0.6% ±0.5% ≤0.6% ≤0.25% 10 ml 100 µl 100 µl 2,000 µl 2,000 µl 200 µl 2,000 µl ±0.5% ±0.5% ≤0.6% ≤0.25% 25 ml 250 µl 250 µl 5,000 µl 5,000 µl 500 µl 5,000 µl ±0.4% ±0.3% ≤0.6% ≤0.25% 50 ml 500 µl 500 µl 10,000 µl 10,000 µl 1,000 µl 10,000 µl ±0.3% ±0.3% ≤0.5% ≤0.25% For Repeater stream/Xstream and Combitips Plus system 1 2 3 0.1 ml 1 µl 0.1 µl 0.1 ml 100 µl 10 µl 100 µl ±1.6% ±1.0% ≤2.5% ≤0.5% 0.2 ml 2 µl 0.2 µl 0.2 ml 200 µl 20 µl 200 µl ±1.3% ±1.0% ≤1.5% ≤0.5% 0.5 ml 5 µl 0.5 µl 0.5 ml 500 µl 50 µl 500 µl ±0.9% ±0.9% ≤0.8% ≤0.3% 1 ml 10 µl 1 µl 1 ml 1 ml 100 µl 1,000 µl ±0.9% ±0.6% ≤0.55% ≤0.2% 2.5 ml 25 µl 2.5 µl 2.5 ml 2.5 ml 250 µl 2,500 µl ±0.8% ±0.5% ≤0.45% ≤0.15% 5 ml 50 µl 5 µl 5 ml 5 ml 500 µl 5,000 µl ±0.8% ±0.5% ≤0.35% ≤0.15% 10 ml 100 µl 10 µl 10 ml 10 ml 1,000 µl 10,000 µl ±0.5% ±0.4% ≤0.25% ≤0.15% 25 ml 250 µl 25 µl 25 ml 25 ml 2,500 µl 25,000 µl ±0.3% ±0.3% ≤0.25% ≤0.15% 50 ml 500 µl 50 µl 50 ml 50 ml 5,000 µl 50,000 µl ±0.3% ±0.3% ≤0.25% ≤0.15% The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) according to EN ISO 8655 is valid only when original Eppendorf® Combitips Plus are used. U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,729 U.S. Pats. 5,620,660/5,620,661 For ordering information, see next page. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing Combitips Plus® for Repeater® Plus and Repeater stream/Xstream 70 Manual and electronic dispensers 1 | Consumables | Pipetting | Dispensing Combitips Plus® Volume table for using Combitips Plus1 with the Repeater® Plus2: The positions and dispensing steps that are marked in bold are based on Combitips Plus used with the original Repeater pipette Position of the volume selection dial: 0.5 1/1 1.5 2/2 2.5 3/3 3.5 4/4 4.5 5/5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 14 12/11 11 10/8 9 8 7 7 6 6 5 5 5 5 Maximum number of dispensing volumes: 100 50/48 33 25/23 20 16/15 Combitips Plus with the following volumes: 1 2 3 0.1 ml 1 µl 2 µl 3 µl 4 µl 5 µl 6 µl 7 µl 8 µl 9 µl 10 µl 11 µl 12 µl 13 µl 14 µl 15 µl 16 µl 17 µl 18 µl 19 µl 20 µl 0.2 ml 2 µl 4 µl 6 µl 8 µl 10 µl 12 µl 14 µl 16 µl 18 µl 20 µl 22 µl 24 µl 26 µl 28 µl 30 µl 32 µl 34 µl 36 µl 38 µl 40 µl 0.5 ml 5 µl 10 µl 15 µl 20 µl 25 µl 30 µl 35 µl 40 µl 45 µl 50 µl 55 µl 60 µl 65 µl 70 µl 75 µl 80 µl 85 µl 90 µl 95 µl 100 µl 1.0 ml 10 µl 20 µl 30 µl 40 µl 50 µl 60 µl 70 µl 80 µl 90 µl 100 µl 110 µl 120 µl 130 µl 140 µl 150 µl 160 µl 170 µl 180 µl 190 µl 200 µl 2.5 ml 25 µl 50 µl 75 µl 100 µl 125 µl 150 µl 175 µl 200 µl 225 µl 250 µl 275 µl 300 µl 325 µl 350 µl 375 µl 400 µl 425 µl 450 µl 475 µl 500 µl 5 ml 50 µl 100 µl 150 µl 200 µl 250 µl 300 µl 350 µl 400 µl 450 µl 500 µl 550 µl 600 µl 650 µl 700 µl 750 µl 800 µl 850 µl 900 µl 950 µl 1.0 ml 10 ml 100 µl 200 µl 300 µl 400 µl 500 µl 600 µl 700 µl 800 µl 900 µl 1.0 ml 1.1 ml 1.2 ml 1.3 ml 1.4 ml 1.5 ml 1.6 ml 1.7 ml 1.8 ml 1.9 ml 2.0 ml 3 25 ml 250 µl 500 µl 750 µl 1.0 ml 1.25 ml 1.5 ml 1.75 ml 2.0 ml 2.25 ml 50 ml3 500 µl 1.0 ml 1.5 ml 2.0 ml 3.0 ml 4.0 ml 2.5 ml 3.5 ml 4.5 ml 2.5 ml 2.75 ml 5 ml 5.5 ml 3.0 ml 3.25 ml 3.5 ml 3.75 ml 4.0 ml 4.25 ml 4.5 ml 4.75 ml 6.0 ml 7.0 ml 8.0 ml 9.0 ml 6.5 ml 7.5 ml 8.5 ml 9.5 ml 5 ml 10 ml U.S. Pats. 5,620,660/5,620,661 U.S. Pats. 5,620,660/5,620,661/5,591/408/5,573,729 25 ml and 50 ml Combitips Plus must be used with the supplied adapter. Ordering information Description Combitips Plus tips Catalog No. List USD 0.1 ml 0.2 ml 0.5 ml 1 ml 2.5 ml 5 ml 10 ml 25 ml 50 ml 25 ml adapter (1 piece), blue 50 ml adapter (1 piece), dark grey 25 ml adapter (set of 7) 50 ml adapter (set of 7) Combitips Plus tips assortment pack, includes five of each size plus one 50 ml and one 25 ml adapter 022265954 022266004 022266101 022266209 022266306 022266403 022266501 022266551 022266608 022266993 022266705 022266624 85.00 Combitube (set of two), aspirating aid for removing liquids from volumetric flasks and tall bottles; Combitips Plus tips sizes 5 ml, 25 ml and 50 ml 022261550 53.00 Standard (set of 100) $105.00 105.00 105.00 105.00 105.00 105.00 105.00 130.00 130.00 19.00 19.00 Catalog No. List USD Catalog No. List USD Sterile Eppendorf Biopur®1 (10 bags of (individually wrapped, 10 tips = 100 tips) set of 100) 022267001 $132.00 022496000 $150.00 022267108 132.00 022496026 150.00 022267205 132.00 022496042 150.00 022267302 132.00 022496069 150.00 022267400 132.00 022496085 150.00 022267507 132.00 022496107 150.00 022267604 132.00 022496123 150.00 022267655 155.00 022496131 171.00 022267701 155.00 022496140 171.00 022496158 022496166 117.00 117.00 Eppendorf Biopur Combitips (individaully wrapped) are guaranteed to be sterile, Rnase-, DNA-, ATP- and pyrogen-free. 1 For pipette service and maintenance information, see pages 30 and 31. Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Manual and electronic dispensers 71 Mounting rack for Combitips Plus® utoclavable box with tray for storing up to 10 Combitips A Plus2 tips from 0.1 ml to 10 ml sizes ‡‡ Particularly suitable for storing sterile products ‡‡ Can also be used as a stand for Repeater® Plus3 pipette ‡‡ Allows tips to be attached single-handedly ‡‡ Detachable sliding lid ‡‡ Nonslip stand ‡‡ Durable construction ‡‡ Labeling surface ‡‡ Ordering information Description Catalog No. Combitips Rack, for mounting Combitips Plus tips 022268007 List USD $35.00 Description To save time and fully enjoy the improved convenience of the Repeater Plus and Repeater stream/Xstream, we have developed the Combitips Rack—a box for 10 Combitips Plus tips that helps you to easily and single-handedly attach the tips. 2 3 U.S. Pats. 5,620,660/5,620,661 U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,729 Combitips® For use only with original Repeater and Repeater Plus/8 accessories. Combitips form a reliable and precise system for positive displacement dispensing in combination with the original Repeater. Ordering information Description Catalog No. Combitips, 0.05 ml set of 50, plus 96 x 10 µl pipette tips, autoclavable 022260952 List USD $149.00 Combitips, Standard, set of 100 1.25 ml 022261100 125.00 12.5 ml 022261401 125.00 1.25 ml 022495101 161.00 12.5 ml 022495208 161.00 Combitips, Eppendorf Biopur®, individually wrapped, set of 100 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing Product features 72 Manual and electronic dispensers 1 | Consumables | Pipetting | Dispensing Dispensing acids, alkalis and organic solvents with the Repeater® Plus pipette We have checked a number of acids, alkalis and organic solvents with a variety of viscosities and densities in our laboratories to provide you with practical data in this regard. Liquid Conc. 100 µl 100 µl 1,000 µl 1,000 µl [d] [cv] [d] [cv] Vapor pressure at 20 ºC [hPa] Density at 20 ºC [g/cm3] Viscosity at 20 ºC [mPa s] Double-distilled water 100% ±0.6 ≤0.6 ±0.5 ≤0.25 23 1 1 Acetone 100% 0.4 0.5 –0.2 0.3 240 0.79 0.32 Acetonitrile 100% –0.4 1.1 –0.6 0.2 97 0.79 0.31 [25 ºC] Formid acid 98% –1.7 0.3 –1.9 0.2 42 1.22 1.8 Ammonium hydroxide 32% 1.7 0.5 2.4 0.8 837 0.89 1.3 [26%] Aniline 100% –1.8 0.8 –1.3 0.3 0.5 1.03 4.4 Dimethyl formamide (DMF) 100% 0.4 0.6 0.3 0.1 3.77 0.94 0.82 Dimethyl sulfoxide (DMSO) 100% 0.1 0.7 0.1 0.2 0.56 1.1 2.14 Ethyl acetate 100% –0.4 1.0 –0.4 0.2 97 0.9 0.44 Ethanol 98% –0.1 0.6 0.1 0.1 58 0.78 1.20 Formaldehyde 37% 0.8 0.3 0.8 0.1 1.3 1.08 2.5 [25 ºC] Isopropanol 99% –0.8 1.4 –0.3 0.2 43 0.78 2.2 Perchloric acid 60% –2.0 0.5 –1.9 0.1 9 1.53 - Phosphoric acid 85% –1.7 0.9 –1.9 0.3 2 1.71 47 Nitric acid 65% –1.3 0.3 1.9 0.1 9.4/9 1.41 1.49 [40%] Hydrochloric acid 37% –2.1 0.5 –1.7 0.3 213 1.19 2.0 Sulfuric acid 95–97% –2.6 0.6 –4.4 0.3 0.0016 1.84 26.9 Tetrachloromethane 100% –0.5 1.0 –0.2 0.2 122 1.59 0.96 Tetrahydrofuran (THF) 100% –2.7 0.7 –0.3 0.2 173 0.88 0.48 Toluene (methyl benzene) 100% –0.7 0.9 –1.1 0.1 29 0.87 0.6 Trichloroethylene 100% –0.4 1.9 –0.1 0.3 77 1.46 0.55 Trichloromethane (chloroform) 100% –0.1 0.4 0.3 0.2 213 1.47 0.56 Triethylamine 100% –0.4 0.4 0.1 0.1 69 0.72 0.36 1.1 0.7 1.0 0.2 Mean value able 1: Random [d] and systematic [cv] measurement deviations in percent when dispensing selected liquids T The experiments were performed using a Repeater Plus1 with liquids at room temperature; the results of 10 dispensing operations with a 5 ml Combitips Plus®2 tip are shown. 1 2 U.S. Pats. 5,620,660/5,620,661/5,591,408/5,573,729 U.S. Pats. 5,620,660/5,620,661 Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. Pipet fillers 73 Product features ccurate, safe and fatigue-free series pipetting using A graduated pipets from 0.1 ml to 100 ml ‡‡ Lightweight, balanced design provides comfortable, fatigue-free operation ‡‡ Touch-control adjustable speed for rapid or slow pipetting needs ‡‡ Fine control of the meniscus movement ‡‡ High aspirating speed: 25 ml in 5 seconds ‡‡ One-finger control for the dispensing and blow-out functions ‡‡ Cordless, works for up to seven hours after recharging ‡‡ Autoclavable pipet adapters and filter holders ‡‡ Space-saving wall holder ‡‡ 1-year warranty ‡‡ Ordering information Description Catalog No. Easypet, with transformer, wall holder and 2 membrane filters/0.45 µm 022230204 List USD $385.00 Membrane filter, for Easypet, 0.45 µm with black lettering, set of 5 022232002 23.00 Membrane filter, for Easypet, 0.45 µm with blue lettering, set of 10 022231006 55.00 NiCd batteries KR 15/30 (qty. 3) 022236016 33.00 Wall-mountable holder 022230905 27.00 Pipet Helper® manual pipet filler Product features afe and convenient filling and dispensing of glass and S plastic pipets ‡‡ Easy-pull slide lever applies vacuum to eliminate the hand stress caused by conventional “squeeze bulb” apparatus ‡‡ Single rocker-switch controls aspiration, dispensing and vacuum to save time ‡‡ Disposable, in-line filter prevents contamination ‡‡ Filters and pipet connectors can be autoclaved for decontamination ‡‡ Easily disassembled for maintenance and cleaning ‡‡ Packaged in 100% recyclable material; for more information on epGreen products, see pages 312 and 313. Description Catalog No. List USD Pipet Helper, with filter disk 022230000 $75.00 Filter Disk, 3 µm, pkg. of 10 022231103 49.00 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Easypet® electronic pipet filler 74 Bottle-top dispensers and burets Varispenser® plus/Varispenser 1 | Instruments | Pipetting | Dispensing Description Our Varispenser and Varispenser plus are ideal for dispensing aliquots of liquid from large supply bottles. Their smart design make them capable of extracting reproducible dispensing volumes with no reagent waste. Their tight seal provides the best possible protection against contact with caustic media, such as solvents, and they are chemically resistant and fully autoclavable for the utmost in safety for you and your lab. Product features Extensive volume range (6 sizes) PFA-sealing of the slide piston prevents jamming ‡‡ Wiping piston design prevents crystallization of liquid ‡‡ Highly resistant to chemicals ‡‡ Rapid volume setting using precise graduation scale ‡‡ Easy disassembling and cleaning ‡‡ Telescopic filling tube for use with most bottles ‡‡ 2-year warranty ‡‡ ‡‡ Ordering information Varispenser: Bottletop dispenser, for external bottle threads of 32 mm (sizes 1, 2 and 3) or 45 mm (sizes 4, 5 and 6), complete with telescopic tube, tool and 3 adapters (see below). Quality certificate. Varispenser plus: same as Varispenser, plus recirculation valve, valve toggle and media-specific fine adjustment. Ordering information Size Volume range With 3 adapters for outer diameters (mm) Dispensing steps Inaccuracy1 Imprecision1 Catalog No. List USD Varispenser (adjustable-volume) 1 0.5–2.5 ml 28, 40, 45 0.05 ml ±0.6% ≤0.1% 4960000019 $421.00 2 1–5 ml 28, 40, 45 0.1 ml ±0.5% ≤0.1% 4960000027 421.00 3 2–10 ml 28, 40, 45 0.2 ml ±0.5% ≤0.1% 4960000035 421.00 4 5–25 ml 32, 38, 40 0.5 ml ±0.5% ≤0.1% 4960000043 566.00 599.00 5 10–50 ml 32, 38, 40 1 ml ±0.5% ≤0.1% 4960000051 6 20–100 ml 32, 38, 40 2 ml ±0.5% ≤0.1% 4960000060 887.00 ±0.6% ≤0.1% 4962000016 421.00 421.00 Varispenser (fixed-volume) Bottletop Dispenser, 1 ml Bottletop Dispenser, 5 ml ±0.5% ≤0.1% 4962000024 Bottletop Dispenser, 10 ml ±0.5% ≤0.1% 4962000032 421.00 ±0.6% ≤0.1% 4961000012 445.00 445.00 Varispenser plus (adjustable-volume) 1 0.5–2.5 ml 28, 40, 45 0.05 ml 2 1–5 ml 28, 40, 45 0.1 ml ±0.5% ≤0.1% 4961000020 3 2–10 ml 28, 40, 45 0.2 ml ±0.5% ≤0.1% 4961000039 445.00 4 5–25 ml 32, 38, 40 0.5 ml ±0.5% ≤0.1% 4961000047 614.00 5 10–50 ml 32, 38, 40 1 ml ±0.5% ≤0.1% 4961000055 619.00 6 20–100 ml 32, 38, 40 2 ml ±0.5% ≤0.1% 4961000063 954.00 Discharge tube, spiral 80 cm for Varispenser/Varispenser plus, up to 10 ml 4960824003 Inquire Discharge tube, spiral 80 cm for Varispenser/Varispenser plus, for 25–100 ml 4960825000 Inquire The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) are given according to EN ISO 8655. Bottle-top dispenser is not a substitution for a bottle closure. Volatile liquids can disappear. 1 For more information go to www.eppendorf.com/varispenser Order from your laboratory supply dealer. Product and/or prices change without notice. Yellowappearance, operatingspecifications, button, for use with 200are μl subject pipettetotips Bottle-top dispensers and burets 75 1 | Instruments | Pipetting | Dispensing Top Buret Product features atented recirculation valve for priming without P loss of reagent ‡‡ Voltage supply with long-life primary cells and charging display ‡‡ Dispensing range from 0.01 ml to 999.9 ml ‡‡ Removable electronics unit ‡‡ Modular, service-friendly construction ‡‡ Simple calibration program ‡‡ Discharge tube can be rotated a full 360° ‡‡ Adjustable filling tube horizontal: 142–220 mm; vertical: 10–200 mm ‡‡ Telescopic filling tube for automatically setting the length from 210 mm to 370 mm ‡‡ 2-year warranty ‡‡ Description Our innovative Top Buret sets new standards for manual titration. Its pulse-free dispensing technique allows the desired volumes to be dispensed continuously, thus making titration simpler and safer, with precision values well within required limits. The volume is dispensed by simply rotating the wheels: Model M features a dispensing rate of 2,500 µl per rotation; Model H features a rate of 5,000 µl per rotation. The display readings on both models range from 0.01 ml to 999.9 ml. This bottle-top buret is extremely sturdy, and its user-friendly display and control features provide simple, fatigue-free operation. Application ‡‡ Continuous, pulse-free dispensing technique for rapid titration Ordering information Description Inaccuracy1 Imprecision1 Catalog No. List USD Bottle-top buret with recirculation valve, valve toggle, telescopic filling tube, adjustable discharge tube, three adapters for 40/38/32 mm bottle threads, 2 x 1.5 V microbatteries. Quality certificate. Top Buret M, 2.5 ml per rotation Top Buret H, 5 ml per rotation ±0.2% ±0.2% Dry tube ≤0.1% 4965000017 $1,004.00 ≤0.1% 4965000025 1,004.00 4960851000 Inquire The data for imprecision (random measurement error) and inaccuracy (systematic measurement error) are given according to EN ISO 8655. Bottle-top dispenser is not a substitution for a bottle closure. Volatile liquids can disappear. 1 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 76 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system Easy to use ‡‡ No other automation compares to epMotion – Software can be fully mastered in only a few hours –O ptical sensor for safe operation examines the worktable for correct loading –N o teaching required—labware data is available as free download at www.epMotion.com Accurate Superior automated liquid handling – Pipetting tools with single- and eight-channel Eppendorf® air cushion technology –P ipetting range from 1 to 1,000 µl, always from air to minimize cross-contamination – Typical pipetting precision below 2% CV at 1 µl* ‡‡ Description epMotion automates all your pipetting tasks, delivering the most accurate pipetting and relief from tedious bench work for everyone, everyday. Whether it’s sample or reagent transfer, assay setup or nucleic acid extraction, even with the smallest sample numbers any application will benefit from automation. Pipetting is more accurate, reproducible and completely standardized. Automated pipetting is totally hands-free, creating a more efficient and ergonomic workplace. With six models, two instrument sizes and multiple pipetting deck combinations to choose from, epMotion is the perfect companion for your workload. Transition from manual protocols is simple with predefined protocols, and the powerful labware database ensures that virtually any tube or plate can be used. Flexible Every application, every day – Tubes range from 0.2 ml PCR to 50 ml blue-cap tubes – Plates range from 6 to 96 and 384 wells – Filter and non-filter tips, specially selected for straightness, non-carbonized to reduce costs – Sterile tips available ‡‡ * Eppendorf application note 168: “Measuring the Accuracy and Precision of the epMotion® 5070 Workstation Using the Artel Multichannel Verification System (MVS®).” Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 77 epMotion Capacity Applications 5070 4-position deck and 3 virtual positions General pipetting tasks System description System description 4-position deck and 3 virtual positions, specially designed to fit into a laminar flow hood or fume hood System description System description Genomics applications ‡ Transporting ‡ Stacking ability ‡ 3 thermo modules (optional) ‡ Real-time PCR set-up ‡ PCR set-up with automated amplification ‡ Sequencing set-up ‡ SNP detection ‡ Forensics Examples: All routine pipetting, e.g. sample/ reagent transfer, qPCR or immunoassays 11-position deck and 1 vacuum station Nucleic acid purification ‡ gDNA from various matrixes ‡ Total and viral RNA ‡ Plasmid preparation ‡ BAC purification ‡ PCR and sequencing clean-up ‡ Bacterial DNA Examples: Nucleic acid extraction, Plug‘n’Prep® protocols, filtration, and solid phase extraction page 84 5075 MC ‡ Cell separation ‡ Change of media ‡ Cytotoxicity assays ‡ Growth curves ‡ Soft agar assays ‡ Cell-based assays ‡ Apoptosis assays 12-position deck page 82 5075 VAC Cell culture Examples: Cell culture maintenance and assays page 80 5075 LH Examples: All routine pipetting, e.g. sample/ reagent transfer, qPCR or immunoassays page 78 5070 CB ‡ Serial dilutions ‡ Tube to plate transfer ‡ Reformatting of plates ‡ Concentration normalization ‡ Hit-picking ‡ Reagent transfer ‡ Pooling 9-position deck and 1 position for Mastercycler® ep Complex assays ‡ Luminex® assay set-up ‡ HLA genotyping ‡ Advalytix PCR slide pipetting Examples: Fully automated PCR: set-up and amplification System description page 88 5075 TMX System description 11-position deck and 1 TMX module page 86 Typical applications ‡ Immunoassays ‡ Magnetic bead purification ‡ Hit picking ‡ Cell assays ‡ Nucleic acid preparation Examples: Compound screening, PCR set-up, concentration normalizations In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® applications 78 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5070 Safety door epMotion is fully enclosed. Operation is paused when the door is opened during operation. Optical sensor Checks loading of the worktable for tips (type and number), labware type, and liquid volumes in vessels. 4-position worktable Specially designed for applications such as real-time PCR or immunoassay set-up, tube to plate transfer, serial dilutions, or sample normalization. Pipetting tools High-precision pipetting tools based on the Eppendorf® air cushion liquid handling technology. Unique free-jet dispensing minimizes the risk of cross-contamination. Manually interchangeable between singleand eight-channel tools. Control panel Compact and easy to use, graphical color display. Small footprint Only 65 cm × 48 cm. Fits on even the smallest available lab bench. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 79 Product features 4 labware positions, plus three virtual spaces Pipetting range from 1 µl to 1,000 µl ‡‡ Compatible with micro- and PCR plates ‡‡ Compatible with plates up to 384 wells ‡‡ Compatible with tubes from 0.2 ml to 50 ml ‡‡ Liquid level detection without conductive tips ‡‡ Optical sensor* checks the loaded deck ‡‡ Safety hood with monitored front door ‡‡ Easily exchangeable pipetting tool ‡‡ Innovative control panel ‡‡ PC editor software available ‡‡ Optional PC control version ‡‡ ‡‡ * U.S. Pat. 6,819,437 Applications ‡‡ Serial dilutions ‡‡ Reagent distribution ‡‡ Reformatting into plates ‡‡ Sample transfer from tubes to plates ‡‡ Sample normalization ‡‡ Media change for cell culture ‡‡ Real-time PCR set-up ‡‡ Cell-based assay set-up ‡‡ Immunoassay set-up Description epMotion 5070 is the most compact solution for accurate and reproducible automated pipetting. It is a perfect match for all routine applications, such as serial dilutions, reagent distribution, sample transfer from tubes to plates and sample normalization. Due to its excellent pipetting accuracy, epMotion is the recommended solution for real-time PCR—enabling setups with less reagent volume and excellent reproducibility. Its pipetting technology is based on the classic Eppendorf® piston-stroke pipettes; thus, protocols previously carried out manually are easily transferred to epMotion 5070. Its compatibility to a wide range of predefined consumables also allows established procedures to be maintained. Before every run the optical sensor checks that the required labware is correctly positioned on the deck and it also checks tip type and quantity available. 30 ml and 100 ml reagent reservoirs The reagent reservoirs are “PCR clean” as well as autoclavable, and they can be easily placed in the reagent rack; their special geometry minimizes residual volume and makes them ideal vessels for reagents. epMotion control panel Graphical color display intuitively guides the user through application set-up and execution. Thermorack for Safe-Lock tubes Rack for up to 24 micro test tubes in the formats 0.5/1.5/2.0 ml. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5070 80 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5070 CB Light barrier beam Monitors the closure of the bench hood. Optical sensor Checks loading of the worktable for tips (type and number), labware type, and even liquid volumes in vessels. 4-position worktable Perfect for up to 192 samples per run. Specially designed for cell culture and cell based assays. Compact design Only 65 cm × 48 cm × 63 cm, (W × D × H) fits most laminar flow hoods. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 81 Product features Compatible with almost all culture hoods Innovative light barrier safety lock technology ‡‡ Preprogrammed cell culture applications ‡‡ Sterile pipette tips ‡‡ Autoclavable pipetting tools ‡‡ ‡‡ Applications ‡‡ Cell culture maintenance ‡‡ Cell aliquoting ‡‡ Media exchange ‡‡ Apoptosis testing ‡‡ Cyto-toxicity testing ‡‡ Cell viability testing ‡‡ Soft agar tests Description The epMotion 5070 CB is the first automated pipetting system for cell culture applications allowing all of the benefits of automated pipetting to be available for liquid handling tasks inside a laminar flow or fume hood. Cell maintenance is one of the most repetitive tasks in life science research. The epMotion 5070 CB allows you to automate key processes such as seeding cells, media changes, or performing cytotoxicity tests. It enables you to handle 6-, 24- or 48-well plates or even denser formats from 96 to 384 wells. Almost all single tube sizes such as cryotubes®, safe-lock and blue cap tubes up to 50 ml are supported. epMotion 5070 CB Used on a sterile workbench. Thermorack for cryotubes Pre-cooled rack for up to 24 cryotubes. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5070 CB 82 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5075 LH Gripper Move labware to different positions on the deck. Automated tool exchange Enables the execution of more complex pipetting patterns. 3 thermal element options Peltier-based thermal element for heating or cooling of samples or reagents from 0–110 °C. 12-position worktable For applications requiring higher throughput. epMotion PC Intuitive software guides you through your everyday pipetting tasks. Plate stacking Up to five microplates or two deepwell plates can be stacked on each other by the gripper. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 83 Product features 2 labware positions 1 Automatic exchange of all tools ‡‡ Pipetting range from 1 µl to 1,000 µl ‡‡ Thermal module option ‡‡ Plate gripper option ‡‡ Compatible with tubes from 0.2 ml to 50 ml ‡‡ Compatible with plates up to 384 wells ‡‡ Plate stacking ‡‡ Optical sensor* checks the loaded deck ‡‡ Detection of tip size and tip quantity ‡‡ Safety hood with monitored front door ‡‡ Innovative control panel ‡‡ PC editor software available ‡‡ Optional PC control version ‡‡ Modular system: Upgrade to 5075 VAC, 5075 TMX and 5075 MC possible ‡‡ Liquid level detection with unique optical sensor ‡‡ ‡‡ * U.S. Pat. 6,819,437 Reservoir rack with tube modules Individually configured and temperature controlled. Applications ‡‡ Serial dilutions ‡‡ Reagent distribution ‡‡ Reformatting into plates ‡‡ Sample transfer from tubes to plates ‡‡ Sample normalization ‡‡ Pooling of samples ‡‡ Media change for cell culture ‡‡ Real-time PCR set-up ‡‡ Immuno- and cell-based assay set-up ‡‡ Compound testing ‡‡ Magnetic bead applications Description epMotion 5075 LH is the ideal solution for advanced liquid handling procedures. It offers the same outstanding accuracy and precision as the epMotion 5070, making it an ideal tool for demanding small-volume applications, such as real-time PCR setup, magnetic bead purification and all routine pipetting tasks. The 12-position deck and automatic tool exchange expand the application range to include complex pipetting patterns and higher sample numbers. With heating and cooling options as well as the availability of a gripper for labware transport on the deck, the epMotion 5075 LH is a versatile and flexible automated pipetting system that is able to meet the needs of the most demanding research. Thermal Modules Up to three can be used for active heating and cooling of Thermal Racks, Thermal Blocks and Reagent Reservoir Modules. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5075 LH 84 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5075 VAC Gripper Moves plates and assembles/disassembles the vacuum station without manual interference. Integrated vacuum pump No tubing, wiring, and reservoirs to maintain. Silent operation. 2 thermal element options Peltier-based thermal element for heating or cooling of samples or reagents. 11-position worktable Designed to automate vacuum-based extractions and downstream applications for up to 96 samples. TMX thermal element option For shaking, heating and cooling of samples and reagents. Vacuum station Fully integrated. Adapts automatically to any filter plate type. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 85 Product features Same as epMotion 5075 LH plus: ‡‡ 11 labware positions ‡‡ One vacuum position ‡‡ Integrated vacuum pump ‡‡ Vacuum is fully software controlled ‡‡ Universal spring loaded vacuum frame ‡‡ Plug‘n’Prep® validated protocols Applications Same as epMotion 5075 LH plus: ‡‡ Vacuum based purification applications ‡‡ Solid phase extraction ‡‡ Filtration ‡‡ Predefined and tested methods for: – Plasmid and BAC purification – gDNA from blood and tissue – gDNA from plants – Sequencing and PCR clean-up – Total RNA Description Complex applications, such as nucleic acid purification should not tie lab personnel to the workbench. The epMotion 5075 VAC allows true walk-away automation of purification protocols that rely on vacuum filtration. Pre-validated protocols are available for most standard applications, such as plasmid DNA, genomic DNA and RNA purification. The protocols are predefined on the system and setup takes only a few minutes. Additional customized applications can be developed easily using the control panel or the optional epMotion PC software. The unique Eppendorf silicon mat helps to save time and money by allowing you to use only portions of a 96-well plate when working with smaller sample numbers. The VAC thermo lid also reduces the extraction time substantially by accelerating the ethanol evaporation step. Integrated vacuum station Completely integrated vacuum chamber and pump that adapts automatically to any filter plate. Labware handling Reconfiguration of the vacuum chamber and the transport of labware takes place automatically with the gripper. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5075 VAC 86 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5075 TMX Automated tool exchange Enables the execution of more complex pipetting patterns. Gripper Moves plates and assembles/disassembles the vacuum station without manual interference. 11-position worktable Designed to automate vacuum-based extraction and downstream applications for up to 96 samples. Integrated TMX module Unique 2-D mixing control improves yields and applications results. Vacuum station option For vacuum based extraction procedures. TMX thermal element Peltier-based thermal element for heating or cooling of samples or reagents. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 87 Product features Applications Like epMotion 5075 LH plus an integrated shaker/vortexer with heating and cooling ‡‡ MagBead based extraction ‡‡ Pre-programmed methods for gDNA and RNA ‡‡ gDNA from blood and fabric ‡‡ Sequencing PCR Clean Up from plants ‡‡ Total RNA ‡‡ Unique 2DMix-Control for optimal resuspension ‡‡ Same as epMotion 5075 LH plus: 11 labware positions ‡‡ TMX module—active mixing/vortexing and temperature control ‡‡ Temperature range: 4 °C to 95 °C ‡‡ Automatic lock-in of the labware on the TMX position ‡‡ Pipetting in other deck positions is possible during the mixing steps ‡‡ Description The epMotion 5075 TMX is the most flexible member of the epMotion family of automated pipetting systems. It includes an integrated TMX module to shake, heat or cool samples and reagents. The TMX module can be loaded and unloaded with the gripper and pipetting to the mixer is possible before and after mixing steps. The TMX module is fully software controlled enabling pipetting onto other worktable positions while the mixer is in operation. TMX mixing is based on Eppendorf’s 2DMix-Control technology. Orbital shaking combined with the perfect balance of speed and mixing radius assures the best stirring results, for example in resuspending bacteria pellets or magnetic beads. The 2DMixControl eliminates the need for additional centrifugation steps as the orbital shaking prevents droplets from collecting on the sides of the tube or plate wells. Plate handling capabilities A plate gripper allows for the transport of labware to and from the TMX module. Integrated TMX module Enables the mixing, heating and cooling of samples and reagents. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5075 TMX 88 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® 5075 MC Automated sealing Sealing of plates with reusable silicon-based cycle lock system. Gripper Moves the plate to the Mastercycler® ep. Integrated Mastercycler ep Automated loading of the cycler. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 89 Product features Same as epMotion 5075 LH plus: ‡‡ 9 labware positions ‡‡ Integrated Mastercycler® ep gradient* with motorized lid ‡‡ 96-or 384-well PCR block options ‡‡ Robust, high-quality aluminum or silver block ‡‡ Integrated Mastercycler ep software ‡‡ Unique cycle-lock silicon sealing system ‡‡ Simultaneous PCR reaction and liquid handling possible Applications Same as epMotion 5075 LH plus: ‡‡ Fully automated sample preparation and subsequent amplification in the Mastercycler ep ‡‡ Automated PCR and in 96-/384-well plate formats ‡‡ Automated sequencing reactions in 96-/384-well plate formats ‡‡ In vitro plasmid amplification ‡‡ Automated DNA extraction and profiling of forensic FTA® samples * Mastercycler ep gradient sold separately Practice of the patented polymerase chain reaction (PCR) process requires a license. The Mastercycler is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Description epMotion 5075 MC combines sample preparation and PCR in an enclosed system for automated “walk-away” amplification reactions. This version can be equipped with all motorized lid models of the Eppendorf Mastercycler ep line of thermal cyclers. The cycler software is integrated in the epMotion software, so both system components can easily be programmed with the same innovative control panel. Deck positions can be used for liquid handling protocols while the cycler is running. Ready to go The epMotion 5075 MC and Mastercycler ep provide a great combination for the automation and execution of PCR reactions. Dispensing without cross-contamination Amplification of the 535 bp fragment of the human b-globin gene in a 384-well format, where every other well contains a negative control in a checkerboard pattern. For epMotion ordering information, see page 96. For more epMotion accessories, see page 90. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® 5075 MC 90 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system accessories Reservoir rack Enables the use of up to 7 reagent rack modules or reservoirs. Thermoracks and adapters For use with 96- and 384-well PCR plates on thermal elements. Reservoir rack modules TC Modules for the reservoir rack. Seven module sizes are available to use tubes from 0.2 ml to 50 ml. Temperature-controlled when used with a thermal module. Height adapter Various height adapters enable exact height level adjustment and accelerated processing of microplates. 30 ml and 100 ml reagent reservoir The reagent reservoirs are both “PCR clean,” autoclavable, and can be placed in the reservoir rack. Their special geometry minimizes residual volume. Thermoblock for PCR plates Thermal block holds 96or 384-well PCR plates. Allows for temperature control when used with a thermal module. Racks for single test tubes For micro test tubes, glass, or plastic tubes or various sizes. Gripper for epMotion 5075 For the transport of labware on the worktable and for automatic operation of the vacuum manifold. Thermoadapter DWP 96 For heating or cooling of deepwell plates. Plates can be exchanged by the gripper. Thermal module (only epMotion 5075) For thermoracks and 96- and 384-well PCR plates. Cool or heat up to 3 positions on the deck. Thermoracks for 24 × Safe-Lock 0.5/1.5/2 ml tubes For 24 micro test tubes. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Dispensing tools 3 different dispensing tools are available from either 1–50 µl, 20–300 µl or 40–1,000 µl in either single- or 8-channel formats. For ordering information, see pages 96 to 99. Automated pipetting 91 Accuracy and reproducibility delivered Based on Eppendorf® classic air cushion pipetting technology No tubes, no wires, no air bubbles As simple to operate and maintain as a manual pipette ‡‡ Excellent pipetting precision, typically below 2% CV at 1 µl* ‡‡ Automatic alert when pipetting tools need calibration ‡‡ Ultra-low dead volume pipetting ‡‡ Every tool tested and certified to ISO 85540 ‡‡ ‡‡ * Eppendorf application note 168: “Measuring the Accuracy and Precision of the epMotion® 5070 Workstation using the Artel Multichannel Verification System (MVS®).” epT.I.P.S. Motion Specially selected tips for use in automated systems. Each tip is inspected for straightness prior to packaging. This ensures a perfect handling of 96- and 384-well micro and PCR plates. Box packaging ensures contamination-free tip loading Non-carbonized, pure virgin polypropylene, both tip and box are recyclable ‡‡ Standard, filter, and sterile tips available ‡‡ PCR-clean quality grade for filter tips ‡‡ ‡‡ ‡‡ 50 µl filter tip ‡‡ 50 µl tip without filter ‡‡ 300 µl filter tip ‡‡ 300 µl tip without filter ‡‡ 1,000 µl filter tip ‡‡ 1,000 µl tip without filter In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Consumables | Pipetting | Dispensing epMotion® tools and tips 92 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® Control: two smart options Product features ‡‡ ‡‡ he epMotion PC version with epBlue™ software offers T the most convenient way ever to use an automated liquid handling system he epMotion control panel provides all functions and T Windows® style programming in an ultra-compact design Description epMotion offers you two options to control automated pipetting: the epMotion PC version or the epMotion control panel. Whatever you choose, the innovative epMotion functions make programming easy; pipetting pattern recognition, labware database, and liquid classes are always included. epMotion PC version Product feature ‡‡ he network interface of the integrated PC allows T the remote control of the epMotion via direct ethernet connection. Method files can be shared using the computer network or USB port. epMotion control panel Product features Graphical user interface Drag and drop programming ‡‡ Windows explorer style organization ‡‡ User management ‡‡ Run reports ‡‡ Software preinstalled on the control panel ‡‡ ‡‡ Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 93 Description Simple and clear, epBlue guides you through your everyday pipetting tasks: Choose your objective in the home section and follow the epBlue commands from left to right with unique guiding menus and the consistent, tab-based structure. With the unmatched ease-of-use of epBlue, even complex methods can be generated in minutes. Pipetting pattern recognition, labware database, and liquid classes are built-in and organized for intuitive use. Simply set up your worktable, select the pipetting commands, simulate, and launch. Operating and programming epBlue is fast and easy. ‡‡ ‡‡ he home screen provides fast access to the main T functions and personal list of the recently used methods, while the intuitive graphical user interface provides a consistent, tab-based workflow from left to right ‡‡ Safe operation through 3D run-simulation Menus guide you step by step through the software functions epMotion editor—easy off-line program management epMotion editor A separate, off-line software package for generating and editing methods as well as printing and archiving program processes on your PC. Product features Programming functions analogous to the control panel Graphical user interface identical to epBlue ‡‡ Print function ‡‡ Archiving function ‡‡ Transfer of methods using the MMC card ‡‡ ‡‡ In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Software | Pipetting | Dispensing epMotion® PC software: epBlue™—intuitive operation and programming 94 Automated pipetting epMotion® automated pipetting system 1 | Instruments | Pipetting | Dispensing Technical specifications Set Eppendorf Quality 5070 epMotion® 5070 5075 LH 5075 VAC 5075 VAC epMotion® Width 39 in/98 cm 56 in/140 cm 56 in/140 cm Depth 30 in/75 cm 30 in/75 cm 30 in/75 cm Width 26 in/65 cm 43 in/107 cm 43 in/107 cm Depth 19 in/48 cm 24 in/61 cm 24 in/61 cm Height 25 in/63 cm 27 in/67 cm 27 in/67 cm Width 10 in/25 cm 10 in/25 cm 10 in/25 cm Depth 6 in/15 cm 6 in/15 cm 6 in/15 cm Height 4 in/11 cm 4 in/11 cm 4 in/11 cm Device: 99 lb/45 kg 187 lb/85 kg 198 lb/90 kg Control panel: 3 lb/1.2 kg 3 lb/1.2 kg 3 lb/1.2 kg Voltage: 110–130 V ±10% 110–130 V ±10% 110–130 V ±10% Frequency: 50–60 Hz ±5 % 50–60 Hz ±5% 50–60 Hz ±5% Max. output: 80 W 1,000 W 1,000 W Systematic measurement error: ±15% ±15% ±15% Random measurement error: ≤5% ≤5% ≤5% Systematic measurement error: ±1.2% ±1.2% ±1.2% Random measurement error: ≤0.4% ≤0.4% ≤0.4% Systematic measurement error: ±0.7% ±0.7% ±0.7% Random measurement error: ≤0.15% ≤0.15% ≤0.15% Surface System: Dimensions (W x D x H) Device: Control panel: Weight Power supply Dispensing tools Volume: Volume: Volume: 1 µl 50 µl 1,000 µl in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °C Conductor X, Y, Z Positioning: Systematic measurement error: ±0.3 mm ±0.3 mm ±0.3 mm Random measurement error: ±0.1 mm ±0.1 mm ±0.1 mm Positions in MTP-Format: 4 12 12 Detector Optical confocal infrared detector: contact-free detection of liquid levels, tools used, labware worktables, tip types and quantities Optical sensor:* liquid surface must be 90 ±3° to the vertical plane of the optical sensor Gripper Carrying capacity: ≤1,200 g ≤1,200 g ≤1,200 g Max. output: - - 35 L/min Suction range: - - 0.1–0.85 ±0.05 Pa Suction time: - - 1–99 min Vacuum unit * U.S. Pat. 6,819,437 Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 95 epMotion® automated pipetting system 5075 TMX 5075 MC epMotion® Width 56 in/140 cm 56 in/140 cm 39 in/98 cm Depth 30 in/75 cm 30 in/75 cm 24 in/62 cm Width 43 in/107 cm 43 in/107 cm 26 in/65 cm Depth 24 in/61 cm 24 in/61 cm 19 in/48 cm Height 27 in/67 cm 27 in/67 cm 25 in/63 cm Width 10 in/25 cm 10 in/25 cm 10 in/25 cm Depth 6 in/15 cm 6 in/15 cm 6 in/15 cm Height 4 in/11 cm 4 in/11 cm 4 in/11 cm Device: 209 lb/95 kg 224 lb/102 kg 99 lb/45 kg Control panel: 3 lb/1.2 kg 3 lb/1.2 kg 3 lb/1.2 kg Voltage: 110–130 V ±10% 110–130 V ±10% 110–130 V ±10% Frequency: 50–60 Hz ±5% 50–60 Hz ±5 % 50–60 Hz ±5% Max. output: 1,000 W 1,000 W 70W Systematic measurement error: ±15% ±15% ±15% Random measurement error: ≤5% ≤5% ≤5% Systematic measurement error: ±1.2% ±1.2% ±1.2% Random measurement error: ≤0.4% ≤0.4% ≤0.4% Systematic measurement error: ±0.7% ±0.7% ±0.7% Random measurement error: ≤0.15% ≤0.15% ≤0.15% 5075 LH 5070 CB epMotion® 5075 MC Surface System: Dimensions (W x D x H) Device: Control panel: Weight Power supply Dispensing tools Volume: Volume: Volume: 1 µl 50 µl 1,000 µl in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °C Conductor X, Y, Z Positioning: Systematic measurement error: ±0.3 mm ±0.3 mm ±0.3 mm Random measurement error: ±0.1 mm ±0.1 mm ±0.1 mm Positions in MTP-Format: 10 10 4 Detector Optical confocal infrared detector: contact-free detection of liquid levels, tools used, labware worktables, tip types and quantities Optical sensor:* liquid surface must be 90° ±3° to the vertical plane of the optical sensor Gripper Carrying capacity: ≤1,200 g ≤1,200 g ≤1,200 g Max. output: - - - Suction range: - - - Suction range: - - - Vacuum unit * U.S. Pat. 6,819,437 In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing Technical specifications Set Eppendorf Quality 96 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system Ordering information Description Catalog No. List USD epMotion automated pipetting systems epMotion 5070 with control panel, basic device incl. control panel, mouse, software, waste box, MMC, and reader, 50/60 Hz, 100–240 V 960000005 Inquire epMotion 5070 CB with control panel, epMotion to be operated inside closed containers, basic device incl. control panel, mouse, software, waste box, MMC, and reader, 50/60 Hz, 100–240 V 960000021 Inquire epMotion 5075 LH with control panel, basic device incl. control panel, software, waste box, MMC, and reader, 50/60 Hz, 100–240 V 960020006 Inquire epMotion 5075 VAC with control panel, basic device incl. control panel, software, vacuum system, gripper, waste box, MMC, and reader, 50/60 Hz, 100–240 V 960020014 Inquire epMotion 5075 MC with control panel, basic device incl. control panel, software, cycler bay, gripper, waste box, MMC, and reader, 50/60 Hz, 100–240 V. Mastercycler® not included. 960020022 Inquire epMotion 5075 TMX with control panel, basic device incl. Thermomixer, control panel, mouse, software, waste box, MMC and reader, 50/60 Hz, 100–240 V 960020033 Inquire epMotion 5070 PC version Same as 5070 catalog no. 960000005 but with integrated industrial PC, keyboard, and mouse instead of control panel. Includes monitor and epBlue™ software. 960000111 Inquire epMotion 5070 CB PC version Same as 5070 catalog no. 960000021 but with integrated industrial PC, keyboard, and mouse instead of control panel. Software and monitor not included. 960000200 Inquire epMotion 5075 LH PC version Same as 5075 catalog no. 960020006 but with integrated industrial PC, keyboard, and mouse instead of control panel. Includes monitor and epBlue software. 960020111 Inquire epMotion 5075 VAC PC version Same as 5075 catalog no. 960020014 but with integrated industrial PC, keyboard, and mouse instead of control panel. Includes monitor and epBlue software. 960020222 Inquire epMotion 5075 MC PC version Same as 5075 catalog no. 960020022 but with integrated industrial PC, keyboard, and mouse instead of control panel. Includes monitor and epBlue software. 960020333 Inquire epMotion 5075 TMX PC version Same as 5075 catalog no. 960020033 but with integrated industrial PC, keyboard, and mouse instead of control panel. Includes monitor and epBlue software. 960020444 Inquire epMotion with integrated PC Monitor, 19" TFT monitor to be used with epMotion versions with integrated PC 960021044 Inquire epBlue—epMotion PC software, operating software for epMotion versions with integrated PC, preinstalled 960000309 Inquire 960021002 Inquire Upgrade and retrofit sets MC, for retrofitting an LH version into an MC version VAC, for retrofitting an LH version into a VAC version 960021011 Inquire Software and hardware upgrade set 1, upgrade kit for epMotion 5075 with serial numbers <1,000 960021022 Inquire Hardware upgrade set 2, includes integrated industrial PC, mouse, keyboard, monitor and epBlue software 960021033 Inquire OEM components of the epMotion can be placed at the disposal of developers and manufacturers of similar devices as a basic module on request. Please contact our Customized Solutions department (see page 256). * U.S. Pat. 6,819,437 Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 97 Ordering information Description Catalog No. List USD Dispensing tools Highly precise pipetting heads, for use in the tool holder of the epMotion workstation. Each dispensing tool is completely autoclavable at 121 °C, 1 bar for 20 min. A quality certificate for the measurement results accompanies each tool. TS 50, single-channel dispensing tool for the volume range 1–50 µl 960001010 $2,250.00 TS 300, single-channel dispensing tool for the volume range 20–300 µl 960001028 2,250.00 TS 1000, single-channel dispensing tool for the volume range 40–1,000 µl 960001036 2,250.00 TM 50-8, 8-channel dispensing tool for the volume range 1–50 µl 960001044 3,970.00 TM 300-8, 8-channel dispensing tool for the volume range 20–300 µl 960001052 3,970.00 TM 1000-8, 8-channel dispensing tool for the volume range 40–1,000 µl 960001061 3,970.00 Holder, for 6 dispensing tools 960001109 284.00 epT.I.P.S. Motion pipette tips Pipette tips for automated systems in individual racks, for use with the epMotion. Tip type and size are automatically recognized on the device. 96 epT.I.P.S./rack, 15 racks per set. Without filter, sterile 50 µl, sterile, volume range 1–50 μl, 15 × 96 tips in racks 960050200 139.00 300 µl, sterile, volume range 20–300 μl, 15 × 96 tips in racks 960050202 139.00 1,000 µl, sterile, volume range 40–1,000 μl, 15 × 96 tips in racks 960050204 139.00 Without filter, Eppendorf Quality 50 µl, volume range 1–50 µl, 15 x 96 tips in racks 960050002 118.00 300 µl, volume range 20–300 µl, 15 x 96 tips in racks 960050045 118.00 1,000 µl, volume range 40–1,000 µl, 15 x 96 tips in racks 960050088 118.00 960050201 272.00 Filtertips, sterile and PCR clean 50 µl, sterile, volume range 1–50 μl, 15 × 96 tips in racks 300 µl, sterile, volume range 20–300 μl, 15 × 96 tips in racks 960050203 272.00 1,000 µl, sterile, volume range 40–1,000 μl, 15 × 96 tips in racks 960050205 272.00 960050029 230.00 Filtertips, PCR clean 50 µl, volume range 1–50 µl, 15 x 96 Filtertips in racks 300 µl, volume range 20–300 µl, 15 x 96 Filtertips in racks 960050061 230.00 1,000 µl, volume range 40–1,000 µl, 15 x 96 Filtertips in racks 960050100 230.00 Reservoir rack modules Reservoir rack modules are inserted in a reservoir rack. They can be temperature controlled with a thermal module for heating and cooling. PCR 0.2 ml, for 8 x 0.2 ml PCR tubes 960002601 370.00 PCR 0.5 ml, for 8 x 0.5 ml PCR tubes 960002611 370.00 Safe-Lock, for 4 x 0.5/1.5/2 ml tubes 960002620 385.00 Tubes Ø 12 mm, for 4 x Ø 12 mm tubes 960002630 390.00 Tubes Ø 16 mm, for 4 x Ø 16 mm tubes 960002640 390.00 15 ml Tubes “Blue Cap,” for 4 x Ø 17 mm tubes 960002650 385.00 50 ml Tubes “Blue Cap,” for 2 x Ø 29 mm tubes 960002660 390.00 Reservoir adapter 30 ml, for 1 x epMotion reservoir 30 ml 960002670 155.00 Reservoir adapter 100 ml, for 1 x epMotion reservoir 100 ml 960002680 155.00 Please contact Eppendorf North America to receive a quotation for epMotion automated pipetting systems and accessories. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system 98 Automated pipetting 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system Ordering information Description Catalog No. List USD Ø 17 mm x 100 mm max. length 960002024 $ 355.00 Ø 17 mm x 60 mm max. length 960002156 355.00 Racks For 24 glass or plastic tubes, no temperature control Ø 16 mm x 100 mm max. length 960002032 355.00 Ø 16 mm x 60 mm max. length 960002164 355.00 Ø 15 mm x 100 mm max. length 960002377 355.00 Ø 15 mm x 60 mm max. length 960002326 355.00 Ø 14 mm x 100 mm max. length 960002369 355.00 Ø 14 mm x 60 mm max. length 960002334 355.00 Ø 13 mm x 100 mm max. length 960002041 355.00 Ø 13 mm x 60 mm max. length 960002342 355.00 Ø 12 mm x 100 mm max. length 960002059 355.00 Ø 12 mm x 60 mm max. length 960002351 355.00 Rack for 24 x HPLC tubes, Ø 12 mm x 40 mm max. length 960002380 345.00 Rack for 96 x 1.5/2.0 ml screw-cap tubes, requires 2 positions on the deck 960002318 905.00 Thermorack for 24 × cryotubes® or labelled Safe-Lock tubes 960002491 1,285.00 Height adapters For uniform levels of labware; enables faster processing of the plate 85 mm 960002105 505.00 55 mm 960002113 505.00 40 mm (for epT.I.P.S. Motion pipette tips) 960002121 505.00 epMotion editor, includes editor key, software package for creating and editing methods and for printing out and archiving program sequences on a PC 960000269 2,770.00 epMotion editor, additional license 960000300 1,080.00 MultiMediaCard, for archiving parameters and transporting data between control panel and PC 960002008 86.00 Accessories for epMotion 5070/5075 Waste container, receptacle for used pipette tips, with lid 960002016 113.00 Thermorack for 24 x 0.5 ml Safe-Lock tubes, for a supply of 24 test tubes, temperature control 960002067 1,030.00 Thermorack for 24 x 1.5/2 ml Safe-Lock tubes, for a supply of 24 test tubes, temperature control 960002075 1,030.00 Thermoadapter for Eppendorf Deepwell Plates 96 960002391 1,460.00 Adapter sleeves, 1 set = 25 pieces for reconfiguration of the thermo rack (1.5/2.0 ml) for use with 0.5 ml tubes 960002172 325.00 Assembly plate, to be used with epMotion 5070 CB, supports laminar flow worktable 960002570 480.00 Temperature controlled shaker, upgrade option for the 5075 LH and 5075 VAC 960021055 8,190.00 Please contact Eppendorf North America to receive a quotation for epMotion automated pipetting systems and accessories. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Automated pipetting 99 Ordering information Description Catalog No. List USD 960002520 $ 330.00 Rack LC 20 µl/100 µl, for holding up to 96 x 20 μl or 100 μl LightCycler capillaries, for use with, e.g., Centrifuge 5804/5804 R or 5810/5810 R, set of 2 960002511 2,510.00 Thermorack CB 100 µl, for holding up to 384 x 0.1 ml strip tubes in the Corbett Research Rotor-Gene 3000 960002500 1,345.00 Thermoadapter for PCR plates, 96-well, skirted, for heating or cooling of PCR plates; plates are exchangeable using the gripper 960002199 795.00 Thermoadapter for PCR plates, 384-well, skirted, for heating or cooling of PCR plates; plates are exchangeable using the gripper 960002202 885.00 Thermoadapter Frosty, combination of height adapter and PCR-Cooler for cooling of skirted PCR plates 960002300 780.00 Thermoblock for PCR plates, 96-well, for use with 96 x 0.2 ml tubes or a PCR plate 96 960002083 795.00 Thermoblock for PCR plates, 384-well, for use with a PCR plate 384 960002091 885.00 CycleLock starter set, 1 frame and 8 mats for automated sealing of Eppendorf® PCR plates, PCR clean; can only be used with Mastercyler® ep 960002288 330.00 CycleLock mats, 5 sealing mats, PCR clean, frame not included 960002296 89.00 960002148 170.00 30 ml, set of 50 960051009 205.00 100 ml, set of 50 960051017 300.00 960002237 505.00 Accessories for PCR/real-time PCR Rack Smart, for holding one tube rack of SmartCycler® reaction tubes ® Accessories Reservoir rack, for use with 30 ml and 100 ml reagent reservoirs, holds max. seven reservoirs of 30 ml or 100 ml filling volumes epMotion Reservoir Large volume reservoir, must be used with a reservoir rack. Two sizes: 30 ml or 100 ml maximum volume. 5 reservoirs packed in separate bags, 10 bags per set. All reservoirs are PCR clean (free of human DNA, DNase, RNase and PCR inhibitors) and made of polypropylene. Production batch-tested, certified. Accessories for nucleic acid purification Vac frame holder Vac lid 960002245 345.00 Mat, for vacuum lid 960002407 225.00 Vac frame 1 960002253 1,030.00 Vac frame 2 960002261 1,030.00 Collection Plate Adapter, for handling collection microtubes in microtube racks 960002531 125.00 Channeling Plate Adapter, for vacuum-processing foaming solutions through multiwell plates, set of 10 960002540 57.00 Vac Thermo Lid, for effective drying of filter plates in epMotion 5075 VAC vacuum chamber Note: additional thermal module required 960002551 1,870.00 Thermal module, for heating and cooling microplates and thermal racks 960002181 6,680.00 Gripper, for transporting plates on the deck and for automatic operation of the vacuum chamber 960002270 7,700.00 Gripper holder 960002211 570.00 Accessories for epMotion 5075 only In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Instruments | Pipetting | Dispensing epMotion® automated pipetting system 100 Service products 1 | Service | Pipetting | Dispensing epMotion® Performance Plans Performance Plan Value Choose a plan that’s right for you. Preventive Maintenance is at the heart of every epMotion Performance Plan. It’s a schedule of services designed to check your system and preserve its maximum performance quality. With Advantage Service a preventive maintenance is combined with a single service repair visit including any parts, labor and travel, for a value packaged offering. Our Premium Service Plan is a full service agreement to cover everything on unlimited visits and an annual Preventive Maintenance. epMotion Tool Calibration Dispensing tools are precision devices. For this reason they should be regularly maintained and calibrated, which will help to ensure that they continue to perform with high precision and accuracy. Calibration of dispensing tool (gravimetric), thermal module and vacuum chamber S. Calibration of the dispensing tools can be performed according to ISO 8655 with NIST-traceable standards supporting GLP documentation. Performance plans (1-year contract) Preventive Maintenance Advantage Service Premium Cleaning and lubricating the device and components ‡ ‡ ‡ Mechanical inspection and adjustment, if necessary ‡ ‡ ‡ Software update (upon customer request) ‡ ‡ ‡ Replacement of all dispensing tool seals ‡ ‡ ‡ Function test and calibration of level sensor and carrier on all models; plus vacuum chamber of epMotion 5075 VAC ‡ ‡ ‡ Function test according to service test methods ‡ ‡ ‡ Full documentation ‡ ‡ ‡ Number of emergency visits included 0 1 unlimited Warranty extension (parts and labor) – 12 Months 12 months Preventive maintenance visits per plan year 1 1 1 Please contact Eppendorf Customer Support at 800-645-3050, menu option 1, for a quotation. Order direct from Eppendorf North America. Product appearance, specifications, and/or prices are subject to change without notice. Long term benefits of Performance Plans ‡‡ Confidence in results ‡‡ Assurance of performance over an extended lifetime ‡‡ GLP compliance 101 Features of Eppendorf® Performance Plans ‡‡ Complete satisfaction and quality delivered by Eppendorf experts ‡‡ Complete programs—easy to understand, easy to budget, no surprises ‡‡ Complete documentation management—everything you need to be in compliance For more information go to www.eppendorfna.com/service epMotion Tool Calibration Service Summary Procedures Advantage epMotion Tool Certification epMotion Tool GLP/ GMP Custom Internal/external inspection and cleaning of pipetting tool Yes Yes Replacement of worn or broken parts, if needed* Yes Yes Pipette calibration performed using NIST-traceable, semi-micro (0.00001g) balance Yes Yes Number of measuring points: 4 low/mid/high per channel As requested, based on customer requirements; may include “as found” data. Min 4/channel Max 10/channel measured Yes Yes recorded Yes Yes Mean volume, inaccuracy and imprecision values measured and recorded Yes Yes Calibration label applied to pipetting tool Yes Yes Calibration report supplied Yes Yes single-channel tool 4 low/mid/high multichannel tool Individual weighing results: Single-channel pipetting tool (spare parts additional if needed) Catalog No. List USD (shipping not included) 901510597 901510600 $85.00 (includes 4/4/4 as returned only) $85.00 plus $0.50 per extra reading Multichannel pipetting tool (spare parts additional if needed) Catalog No. List USD (shipping not included) 901510599 901510602 $150.00 (includes 4/4/4 as returned only) $150.00 plus $0.50 per extra reading * Spare parts additional if needed. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: [email protected] · www.eppendorf.com 1 | Service | Pipetting | Dispensing Service products
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