ProgRes® microscope cameras

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ProgRes® microscope cameras
Quick-Start-Guide
Getting started
Congratulations to your ProgRes® microscope camera!
This Quick-Start-Guide will help you to quickly install the ProgRes®
microscope camera and the ProgRes® CapturePro application software,
and will give you an overview over their main functions. For more
information please refer to the ProgRes® CapturePro - Software manual
which you will find on the installation CD and in the “Help“-menu of
the ProgRes® CapturePro application software.
English
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Important note: For the safe operation of your ProgRes®camera, please observe the safety advice and the hardware
requirements described in the instruction manual included in
your delivery.
Easy installation
•Mount your ProgRes® microscope camera to the microscope by
means of the C-Mount adapter.
•Click the file “ProgRes® CapturePro.exe“ and follow the advice in the
installation routine to install the ProgRes® CapturePro software.
•Connect the ProgRes® microscope camera to your computer by
means of the FireWire- or USB-cable.
Desktop-computer
Power is supplied via FireWire- or USB-cable; you do not need to install
any further equipment.
Laptop-computer
When using a ProgRes® FireWire-camera with a Laptop computer, power supply has to be provided additionally by using a repeater.
Repeater
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ProgRes® Quick-Start-Guide 001
Hot Keys
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Note: Use only accessories which are included in your delivery or which are explicitly recommended by the manufacturer.
Software start
Double-click on the ProgRes® CapturePro-Icon
. After starting the
software, the live image will be displayed and you can immediately start
working with the application software.
English
Hot Keys
F2
Capture
F3
Live
F4
Snapshot: Capture an image in the Live mode and save
it directly in the live image resolution.
F11
Activate or deactivate Full screen mode.
CTRL+DEL
Delete marked form (context menu “Measure“).
SHIFT+
CTRL+DEL
Delete all forms (context menu “Measure“).
CTRL+B
Set marked form to background.
CTRL+F
Set marked form to foreground.
CTRL +S
“Burn“ overlay into the image.
+/-
Zoom in or out the image in Live and Capture mode.
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Note: This function is not available in
“Stretch“ mode.
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5 Steps to capturing a perfect image
5 Steps to capturing a perfect image
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Image without white balance
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Image with white balance
Set Auto Exposure - Register tab “Exposure“:
“Continuously“ is activated per
default. To adjust exposure once,
click “Auto Exposure“
in the icon
bar.
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Set white balance:
•Click “White balance“
in the icon bar. Select a position in the image
which has a neutral grey tint and which is not overexposed.
•Click “Reset white balance“
to reset the white balance.
Background information for setting a suitable white balance:
Ideally, the highest RGB-value of the selected position is approximately 230.
The maximum RGB-value of 255 is not suitable for a white balance. The RGBvalues of the cursor position are displayed in the status bar in the lower right
corner of the image window.
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Note: When resetting the white balance, the image will be reset to
default values and can have a green tint. In this case, carry out the
white balance again.
ProgRes® Quick-Start-Guide 001
5 Steps to capturing a perfect image
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Set image resolution:
The image resolution is preset for
your camera type. You can also
individually set the image resolutions
in the dropdown listboxes in the register tab “Settings“.
Select target folder and image name:
Click “Select...“ and select the
storage place for the images in the
explorer. The storage places selected
in the current session will be displayed in the dropdown listbox “Target
folder“.
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English
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Select image name and saving
format in the respective text box or
dropdown listbox. A current number
will be added to each image.
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Capture images:
When you are pleased with the settings, click “Capture“
. The image
will be saved in the selected folder with the set resolution and can be loaded
and displayed easily by using the “Gallery“-function
.
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Icon bar
Icon bar
Icon bar
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Note: If not all icon bar elements are displayed due to a too low
image resolution, please press the F11-key.
English
Capture: Capture image
Live / Freeze: Start or stop live image display.
Print Image: Print or export up to 6 images in PDF-format directly
from the application software.
Load Image: Load a saved image.
Gallery: Show or hide image gallery.
Stretch to Window: Fit displayed image to the size of the
window.
True Pixel Size: Display image in 1:1-scale or select scale from the
drop-down listbox.
Zoom In / Zoom Out: Zoom image in or out.
Hand-Tool: Shift the displayed image section.
White Balance: Set white balance in the live image.
Auto White Balance: Set automatic white balance in the live
image.
Reset White Balance: Reset created white balance.
Black Balance: Create black balance in the live image.
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ProgRes® Quick-Start-Guide 001
Icon bar
Reset Black Balance: Reset created black balance.
Focus: Set focus in the live image.
Auto Exposure: The exposure time in the live image is adjusted
automatically once.
English
ROI for Auto Exposure: Select an image area. Based on this area,
Auto Exposure for the entire image will be calculated once.
Select Crop: Select image region for live and captured image.
Switch to B/W resp. RGB mode: Display image in Color mode or
in Black / White mode.
Scale: Show measurement scale.
Annotation: Insert text or pointer annotation into the image.
Measure: Insert measurement shape into the image.
Select Shape(s): Mark and select overlay element (annotation or
measurement shape).
Select Camera: Select camera and display serial number.
Help: Display program information and manual.
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Settings
Settings: Set general settings for image capture
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Image resolution: Set resolution for
live image and captured image.
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Mirror / Rotate: Change orientation
of the live image and the captured
image.
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File options: Set target folder in
the explorer and in the dropdown
listbox. The images captured in the
current session are displayed in the
dropdown listbox.
English
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Auto Save: Save images automatically without prior prompting.
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Display image after Capture:
To achieve higher image capture
rates in Timelapse mode, please
deactivate this function.
o
Image name / Image format: Insert
the image name in the dropdown listbox and select the format in which
the image will be saved. The images
will be saved under this name and a
current number will be assigned to
each image.
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Note: Please do not use any blank spaces or special characters in the
image name (register tab “Settings“), otherwise you will not be able
to capture and save images!
ProgRes® Quick-Start-Guide 001
Exposure
Exposure: Set exposure settings
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Exposure control: Insert the exposure
time in ms into the text box, or use
the slider bar to adjust the exposure
time.
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Normalize exposure time for binning:
In Binning mode, the exposure time
for the captured image will be displayed here.
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Auto Exposure: Exposure will either be
continuously adjusted over the entire
image or it will be calculated based
on a selected region of interest (ROI).
By adjusting the intensity with the
slider the image is set generally
“brighter“ or “darker“.
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English
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Gain control: The image signal can
be amplified and exposure time can
be reduced to achieve a higher live
image frame rate.
n
Minimize gain during capture:
When capturing an image, gain will
be converted into exposure time, and
background noise in the captured
image will be minimized.
o
Overexposure: Overexposed image
regions will be highlighted in color.
ProgRes® Quick-Start-Guide 001
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Color
Color: Set color settings
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Color adjustment: Set the balance
between the RGB-basic color and
the complementary CMYK-color.
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Gamma: Use the slider bar to adjust
the gamma value.
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Contrast / Brightness / Saturation:
Adjust contrast, brightness and saturation by using the slider bars.
English
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Note: For an evenly balanced high-contrast color reproduction use
the preset gamma value 0.5. For the linear reproduction of image
data set the gamma value to 0.33.
ProgRes® Quick-Start-Guide 001
Timelapse
Timelapse: Time-controlled image capture
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Start: Select whether the image
capture is started immediately or with
a time delay - insert the delay time if
necessary.
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Time control: Image capture is carried
out either as fast as possible or within
a preset interval.
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Save images: Save the images as single files or as a video sequence. The
frame rate is either synchronized to
the recording time or it is individually
set by the user.
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Max. image count: After reaching
the number of images inserted here,
image capture will be stopped. The
maximum image count depends from
the storage capacity of your computer.
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English
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Settings for fast image recording:
•Deactivate “Fit to screen“.
•Set BMP file format.
•Deactivate “Display image after capture“ in the register tab “Settings“.
•Capture images as single files.
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Measure
Measure: Create a measurement calibration
First, place the calibration standard on
your microscope.
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Calibrations: Click “Add“ and draw
the calibration line. Select length or
angle unit in the dropdown listbox.
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Name: Insert the name of the calibration into the text box.
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Length: Insert the length of the calibration line. This value refers to the
distance referenced by the calibration
line.
English
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General style: Select font, font color,
line width and line color.
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Correction / Draw: Click “Correction“
to modify the existing calibration.
Click “Draw“ to draw a new calibration line.
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When clicking “OK“, the lower area
of the register card will change and
the data of the current calibration will
be displayed.
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Current calibration:
When selecting a calibration in the
dropdown listbox, the data of this calibration will be displayed in the lower
area of the register tab.
o
ProgRes® Quick-Start-Guide 001
Measure
Measure: Set and edit overlay
Click “Measure“ in the icon bar and select the measurement
shapes (overlay elements) from the dropdown listbox.
All measurement shapes in the image can be listed in a
table, which will be displayed when clicking “Show results“
in the dropdown listbox of the button “Measure“.
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Length / Angle: Set length and angle
unit for the measurement shape.
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Merge calibration with image:
Merge the overlay into the image and
save it as part of the image file.
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Measurement shape and ID
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General style: Set font, font color, line
width and line color for the measurement shape and the caption.
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Live form parameter: Select live parameter display of the shape.
English
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Fluorescence
Fluorescence: Capture and save fluorescence images
English
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Filter: Insert dye name and emission of
the filter or activate brightfield image
(SHIFT + * + name).
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Exposure time: Use the slider or insert
exposure time when prompted at the
start of the routine.
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Start: Start fluorescence routine. Follow the advice in the dialog boxes.
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Name of merged image:
Insert name for merged image.
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Name of project folder: Insert name
for fluorescence project folder.
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Merge On / Merge Off: Merge captures of each filter to one image. Click
“Save“ to save merged image.
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Shift image: Shift single filter captures
in a merged image.
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Important note: Please do not use any blank spaces or special characters in the image name (Register tab “Settings“). Set the gamma
value to 0.33 (Register tab “Color“).
Merge brightfield image with fluorescence images:
•Insert SHIFT + * + name into the textbox “Filter“.
•Mark the checkbox to activate the use of the brightfield image.
•The brightfield image will be captured as part of the fluorescence routine.
•The brightfield image can also be loaded by clicking the folder symbol.
Finish the fluorescence project by clicking “Done“.
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ProgRes® Quick-Start-Guide 001
Tools
Tools: Functions for improving image quality
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Shading correction: Click “Create /
Delete...“ and follow the advice in the
dialog windows.
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Reset: Reinitialize camera connection.
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Scanner: Start scanner calibration only available for scanner cameras.
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Trigger: Set configuration for Trigger
In and Trigger Out - only available for
trigger cameras.
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Activate cooling: Activate or deactivate cooling - only available for
cooled cameras.
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User profile: Save and load user
profile.
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English
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Options: Hide register tabs and set
language.
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Background information on the user profile:
The current settings can be saved in the user profile. The user profile “mylast.
cxp“ is continuously updated. To permanently save settings in a user profile,
click the button “Save as...“ and insert the name of the profile. Saved user
profiles can be directly selected and loaded from the status bar.
ProgRes® Quick-Start-Guide 001
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Text Stamp
Text Stamp: Insert caption in the image
English
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Activate or deactivate text stamp.
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Insert name for text stamp.
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Set position for text stamp.
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Display all text stamps and merge
them with the image when clicking
„Capture“.
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Merge text stamp into the loaded
image and save image with overlay.
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Set font and font color.
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ProgRes® Quick-Start-Guide 001
Multifocus
Multifocus: Create image with high depth of focus
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Start: Click “Start“ to start multifocus
capture.
Save: Save merged multifocus image.
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Done: The multifocus session is
finished.
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English
Click “Capture“ to capture an image.
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Status:
Green: Please click “Capture“.
Yellow: Please click “Start“.
Red: Please wait.
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Cut border area:
Cut borders where undesired effects
(artifacts) can occur.
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Note: Only the final merged multifocus image will be saved as a
BMP- or TIFF-file when clicking “Save“. Intermediate steps will not be
saved.
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JENOPTIK l Optical Systems
Business Unit Optoelectronic Systems
JENOPTIK Optical Systems GmbH
Goeschwitzer Str. 25
07745 Jena,
Germany
+49364165-2143
+49364165-2144
[email protected]
www.jenoptik.com/progres
014102-012-98-07-pg-001-1106
Phone.:
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