TC-512 - Phenix Research Products

TC-512 - Phenix Research Products
TC-512
OPERATOR’S MANUAL
Issue 1.
Date of issue:
1st August 2003
 The Techne logo is the trademark
of Techne and Techne Inc.
© Techne, 2003
TC-512
OPERATOR’S
MANUAL
Contents
TC-512 Operator’s Manual
page
SAFETY AND INSTALLATION
Declaration of conformity
English
Français
Deutsch
Español
THE TC-512
Warning
Unpacking
Typical specification at an ambient of 20°C
Working conditions
Uses of the Touchgene
Tubes or reaction vessel
Cleaning your TC-512
Block removal
The heated lid
The memory card
Adjusting the light intensity of the screen
Before switching on
Connections on the back of the unit
Symbols on the screen
Connecting one unit to the computer using an RS232 Cable
Connecting several units to the computer using the daisy chain cables
Some frequently asked questions
POWER UP SCREENS
System user main menu
Program defaults
Numerical input screen
PROGRAMS
New program screen
Template program screens
Existing program screen
Editing programs
The TC-512
Increment or decrement temperature or time
‘Save’ before ‘Run’
RUNNING A PROGRAM
While running programs
Data logs
Printing Data logs
Using “Gensoft”
ADDITIONAL INFORMATION
Interchangeable blocks
Accessories
Replacement parts
3
4
5
6
7
8
8
8
9
10
10
10
11
11
12
13
13
14
14
14
15
15
16
17
18
18
19
20
20
21
23
24
28
30
31
32
33
34
34
36
37
38
39
39
PAGE 1
TC-512
PAGE 2
OPERATOR’S
MANUAL
TC-512
OPERATOR’S
MANUAL
Techne
Duxford
Cambridge
CB2 4PZ
Declaration of Conformity
Techne Unit TC-512 has been designed to comply with the following European Standards:
EN 61326:1998 Electrical equipment for measurement, control and laboratory use. EMC
requirements.
EN 61010-1:1993 Safety requirements for electrical equipment for measurement, control and
laboratory use.
EN 61010-2-010:1995 Particular requirements for laboratory equipment for the heating of
materials.
I have made all reasonable enquiries regarding the unit stated and its conformance to the
following EU directives:
Low Voltage directive, 73/23/EEC and amendment 93/68/EEC, and
EMC Directive 89/336/EEC and amendments 91/263/EEC 92/31/EEC and 93/68/EEC.
To the best of my knowledge and belief these units conform to these directives.
This Declaration is controlled under an ISO 9001:2000 system certificated by BSI Quality
Assurance, certificate number FM13585.
Signature
Name
B C Coombes
Position
Quality Manager
Issue 1
01/08/2003
PAGE 3
TC-512
OPERATOR’S
MANUAL
Introduction
After use
Please read all the information in this booklet before using the
unit.
When you have finished heating samples, remember that
parts of the unit – the tubes, blocks and associated
accessories – may be very hot. Take the precautions listed
earlier.
Warning
HIGH TEMPERATURES ARE DANGEROUS:
they can cause serious burns to operators and
ignite combustible material.
Techne have taken great care in the design of
these units to protect operators from hazards, but users
should pay attention to the following points:
• USE CARE AND WEAR PROTECTIVE GLOVES TO
PROTECT HANDS;
• DO NOT put hot objects on or near combustible objects;
• DO NOT operate the unit close to inflammable liquids or
gases;
• DO NOT place any liquid directly in your unit;
• At all times USE COMMON SENSE.
Operator Safety
All users of Techne equipment must have available the
relevant literature needed to ensure their safety.
It is important that only suitably trained personnel operate this
equipment, in accordance with the instructions contained in
this manual and with general safety standards and procedures.
If the equipment is used in a manner not specified by Techne
the protection provided by the equipment to the user may be
impaired.
All Techne units have been designed to conform to
international safety requirements and are fitted with an
overtemperature cut-out. On some models, the cut-out is
adjustable and should be set to suit the application. On all
other models the cut-out is preset to protect the unit.
If a safety problem should be encountered, switch off at the
mains socket and remove the plug from the supply.
Installation
1. All Techne units are supplied with a power cable. This may
be integral or plug-in.
2. Before connecting the mains supply, check the voltage
against the rating plate. Connect the mains cable to a
suitable plug according to the table below. Note that the
unit must be earthed to ensure proper electrical safety.
Connections 220/240V
110/120V
Live
Brown
Black
Neutral
Blue
White
Earth
Green/yellow Green
The fused plug supplied with the mains lead for use in the
UK is fitted with the following value fuse to protect the cable:
230V UK
10 AMP
The fuse in the unit protects the unit and the operator.
Note that units marked 230V on the rating plate work at
220V; units marked 120V work at 110V. In both cases,
however, the heating rate will degrade by approximately 8%.
The rating plate is on the rear of the unit.
3. Plug the mains cable into the socket on the rear of the unit.
4. Place the unit on a suitable bench or flat workspace, or in a
fume cupboard if required, ensuring that the air inlet vents
on the underside are free from obstruction.
5. Symbols on or near the power switch of the unit have the
following meanings:
I
: mains switch On
O
: mains switch Off
PAGE 4
Guarantee
The unit is guaranteed against any defect in material or
workmanship for the period specified on the enclosed
guarantee card. This period is from the date of purchase, and
within this period all defective parts will be replaced free of
charge provided that the defect is not the result of misuse,
accident or negligence. Servicing under this guarantee should
be obtained from the supplier.
Notwithstanding the description and specification(s) of the
units contained in the Operator’s Manual, Techne hereby
reserves the right to make such changes as it sees fit to the
units or to any component of the units.
This Manual has been prepared solely for the convenience of
Techne customers and nothing in this Instruction Book shall
be taken as a warranty, condition or representation concerning
the description, merchantability, fitness for purpose or
otherwise of the units or components.
User maintenance
NOTE THAT THIS EQUIMENT SHOULD ONLY BE
DISMANTALED BY PROPERLY TRAINED PERSONNEL.
REMOVING THE SIDE, FRONT OR REAR PANELS
EXPOSES POTENTIALLY LETHAL MAINS VOLTAGES.
THERE ARE NO USER MAINTAINABLE PARTS WITHIN
THE EQUIPMENT.
In the unlikely event that you experience any problems with
your unit which cannot easily be remedied, you should contact
your supplier and return the unit if necessary. Please include
any details of the fault observed and remember to return the
unit in its original packing. Techne accept no responsibilty for
damage to units which are not properly packed for shipping: if
in doubt, contact your supplier. See the De-contamination
Certificate supplied with your unit.
1. Cleaning
Before cleaning your unit ALWAYS disconnect from the
power supply and allow to cool below 50° C.
Your unit can be cleaned by wiping with a damp soapy cloth.
Care should be exercised to prevent water from running
inside the unit. Do not use abrasive cleaners.
2. Fuses
Your unit is protected by one or two fuses. These should
only be changed by suitably qualified personnel.
If the fuses blow persistently, a serious fault is indicated
and you may need to return the unit to your supplier for
repair.
Contact Information
For technical, sales or servicing information, contact your local
Techne dealer or,
Techne, Duxford,
Cambridge, CB2 4PZ, United Kingdom.
Telephone:
+44(0)1223 832401
Fax:
+44(0)1223 836838
Service:
+44(0)1223 836950 Out of office hours
e-mail:
[email protected]
Web site:
www.techne.com
or,
Techne Inc, 3 Terri Lane,
Suite 10, Burlington,
New Jersey 08016, USA.
Telephone:
609-589-2560
Toll free:
800-225-9243 ext 306
Fax:
609-589-2571
e-mail:
[email protected]
Web site:
www.techneusa.com
TC-512
OPERATOR’S
MANUAL
Introduction
Après utilisation
Veuillez lire attentivement toutes les instructions de ce
document avant d’utiliser l’appareil.
Lorsque vous avez fini de chauffer les échantillons, n’oubliez
pas que certaines parties de l’appareil - les éprouvettes, leurs
supports et autres accessoires - risquent d’être très chaudes.
Il est donc recommandé de toujours prendre les précautions
citées plus haut.
Avertissement
DANGER DE TEMPERATURES ELEVEES : les
opérateurs peuvent subir de graves brûlures et les
matériaux combustibles risquent de prendre feu.
Techne a apporté un soin tout particulier à la
conception de ces appareils de façon à assurer une protection
maximale des opérateurs, mais il est recommandé aux
utilisateurs de porter une attention spéciale aux points
suivants:
• PROCEDER AVEC SOIN ET PORTER DES GANTS POUR
SE PROTEGER LES MAINS.
• NE PAS poser d’objets chauds sur ou près de matériaux
combustibles.
• NE PAS utiliser l’appareil à proximité de liquides ou de gaz
inflammables.
• NE PAS verser de liquide directement dans l’appareil.
• FAIRE TOUJOURS PREUVE DE BON SENS.
Sécurité de l’opérateur
Tous les utilisateurs de produits Techne doivent avoir pris
connaissance des manuels et instructions nécessaires à la
garantie de leur sécurité.
Important : cet appareil doit impérativement être manipulé par
un personnel qualifié et utilisé selon les instructions données
dans ce document, en accord avec les normes et procédures
de sécurité générales. Dans le cas où cet appareil ne serait
pas utilisé selon les consignes précisées par Techne, la
protection pour l’utilisateur ne serait alors plus garantie.
Tous les appareils Techne sont conçus pour répondre aux
normes de sécurité internationales et sont dotés d’un coupecircuit en cas d’excès de température. Sur certains modèles,
ce coupe-circuit est réglable pour s’adapter à l’application
désirée. Sur d’autres modèles, il est pré-réglée en usine pour
assurer la protection de l’appareil.
Dans le cas d’un problème de sécurité, coupez l’alimentation
électrique au niveau de la prise murale et enlevez la prise
connectée à l’appareil.
Installation
1. Tous les appareils Techne sont livrés avec un câble
d’alimentation qui peut être intégré à l’appareil ou à
raccorder.
2. Avant de brancher l’appareil, vérifiez la tension requise
indiquée sur la plaque d’identification. Raccordez le câble
électrique à la prise appropriée en vous reportant au tableau
ci-dessous. Il est important que l’appareil soit relié à la
terre pour assurer la protection électrique requise.
Connexions
220/240 V
110/120 V
Phase
Marron
Noir
Neutre
Blue
Blanc
Terre
Vert/juane
Vert
Le fusible à l’intérieur de l’appareil est destiné à assurer la
protection de l’appareil et de l’opérateur.
Remarque : les appareils dont la plaque indique 230 V
peuvent fonctionner sur 220 V, et ceux dont la plaque
indique 120 V peuvent fonctionner sur 110 V. Dans les deux
cas cependant, le capacité de chauffage diminuera
d’environ 8 %. La plaque d’identification se trouve à l’arrière
de l’appareil.
3. Raccordez le câble d’alimentation à la prise située à l’arrière
de l’appareil.
4. Placez l’appareil sur un plan de travail ou surface plane, ou
le cas échéant, dans une hotte d’aspiration, en s’assurant
que les trous d’aération situés sous l’appareil ne sont pas
obstrués.
5. Les symboles situés sur ou à côté de l’interrupteur de
l’appareil ont la signification suivante :
O : arrêt
l : marche
Garantie
L’appareil est garanti contre tout défaut ou vice de fabrication
pour la durée figurant sur la carte de garantie, à compter de la
date d’achat de l’appareil. Au cours de cette période, toutes
les pièces défectueuses seront remplacées gratuitement, dans
la mesure où la défaillance n’est pas due à une mauvaise
utilisation, un accident ou une négligence. Toute réparation
sous garantie sera effectuée par le fournisseur.
Malgré la description et les spécifications de l’appareil
données dans le manuel de l’utilisateur, Techne se réserve le
droit d’effectuer les changements nécessaires à l’appareil ou à
tout élément qui entre dans sa composition.
Ce manuel a été exclusivement rédigé à l’attention des clients
de Techne, et aucun élément de ce guide d’instructions ne
peut être utilisé comme garantie, condition ou représentation
concernant la description, commercialisation, adaptation aux
conditions d’utilisation ou autre des appareils ou de leurs
composants.
Entretien utilisateur
IMPORTANT : CET APPAREIL NE PEUT ETRE DEMONTE
QUE PAR DU PERSONNEL QUALIFIE.
LORSQUE LES PANNEAUX AVANT, ARRIERE ET
LATERAUX SONT DEMONTES, L’OPERATEUR EST
EXPOSE A DES TENSIONS QUI PEUVENT ETRE
MORTELLES.
CET APPAREIL NE CONTIENT AUCUN ELEMENT QUI
DEMANDE UN ENTRETIEN DE LA PART DE
L’UTILISATEUR.
Dans le cas peu probable où votre appareil présente un défaut
de fonctionnement auquel il est difficile de remédier, il est
alors préférable de contacter votre fournisseur et, le cas
échéant, de renvoyer le matériel. Veuillez inclure une
description détaillée du problème constaté et retourner
l’appareil dans son emballage d’origine. Techne ne sera pas
tenu responsable des dommages subis par tout appareil dont
l’emballage est inadéquat pour le transport. Pour plus de
sûreté, contactez votre fournisseur. Voir le certificat de
décontamination livré avec le produit.
1. Nettoyage
Avant de nettoyer l’appareil, assurez-vous TOUJOURS que
le câble d’alimentation est déconnecté et laissez la
température redescendre en dessous de 50 °C.
Utilisez un chiffon imprégné d’eau savonneuse pour
nettoyer l’appareil. Veillez à ne pas introduire d’eau dans
l’appareil. N’utilisez pas de produits abrasifs.
2. Fusibles
La protection de l’appareil est assurée par un ou deux
fusibles dont le remplacement ne peut être effectué que par
un personnel qualifié.
Si les fusibles sautent sans arrêt, il s’agit d’un problème
sérieux. Nous vous conseillons dans ce cas de prendre
contact avec votre fournisseur pour réparation.
PAGE 5
TC-512
OPERATOR’S
MANUAL
Einleitung
Nach dem Gebrauch
Bitte lesen Sie diese Bedienungsanleitung komplett bevor Sie
dieses Gerät benutzen.
Vergessen Sie nicht, daß Teile des Gerätes (die Gefäße, die
Blöcke und andere Zubehörteile) nach dem Erhitzen von
Proben noch sehr heiß sein können. Bitte beachten Sie die
oben genannten Vorsichtsmaßnahmen.
Warnung
HOHE TEMPERATUREN SIND GEFÄHRLICH:
sie können dem Bediener ernsthafte Verletzungen
zufügen und brennbare Materialien können sich
leicht entzünden.
Techne hat bei der Konstruktion dieses Gerätes
sehr darauf geachtet, daß der Bediener vor Gefahren
geschützt ist. Dennoch sollten Sie auf die folgenden Punkte
achten:
· SEIEN SIE VORSICHTIG UND TRAGEN SIE
SCHUTZHANDSCHUHE
· Legen Sie heiße Gegenstände NICHT auf oder in die Nähe
von leicht brennbaren Materialien; vermeiden Sie Arbeiten in
der Nähe von leicht entzündbaren Flüssigkeiten oder Gasen.
· Bringen sie KEINE Flüssigkeiten direkt in Ihr Gerät.
· Benutzen Sie immer den normalen Menschenverstand
Sicherheit des Anwenders
Alle Benutzer von Techne Geräten müssen Zugang zu der
entsprechenden Literatur haben, um ihre Sicherheit zu
gewähren.
Es ist wichtig, daß diese Geräte nur von entsprechend
geschultem Personal betrieben werden, das die in dieser
Gebrauchsanweisung enthaltenen Maßnahmen und
allgemeine Sicherheitsbestimmungen und -vorkehrungen
beachtet. Wenn das Gerät anders eingesetzt wird als vom
Hersteller empfohlen, kann dies die persönliche Sicherheit des
Anwenders beeinträchtigen. Die Geräte von Techne
entsprechen den internationalen Sicherheitsbestimmungen
und sind mit einem automatischen Übertemperaturabschalter
ausgestattet. Bei einigen Modellen ist der
Übertemperaturabschalter verstellbar und sollte je nach
Anwendung entsprechend eingestellt werden. Bei allen
anderen Modellen ist der Temperaturschutz voreingestellt um
Schäden am Gerät zu vermeiden. Wenn ein
Sicherheitsproblem auftreten sollte, muß das Gerät
ausgeschaltet und vom Stromnetz getrennt werden.
Installation
1. Alle Techne Geräte werden mit einem Stromanschlußkabel
geliefert. Dieses ist entweder fest mit dem Gerät verbunden
oder zum Einstecken.
2. Vergleichen Sie, ob die Spannung Ihrer Stromversorgung
mit den Angaben auf dem Typenschild des Geräte
übereinstimmen. Verbinden Sie das Stromanschlußkabel
mit einer geeigneten Stromversorgung gemäß der
nächstehenden Tabelle. Achtung: Das Gerät muß geerdet
sein, um die elektrische Sicherheit zu gewährleisten!
Verbindungen 220/240V
110/120V
Stromführend Braun
Schwarz
Neutral
Blau
Weiß
Erde
Grün/Gelb
Grün
Geräte, die für 230 Volt ausgelegt sind, können auch bei
220 Volt arbeiten, Geräte für 120 Volt auch bei 110 Volt. In
beiden Fällen verringert sich die Aufheizrate um ca. 8%.
Das Typenschild befindet sich hinten am Gerät.
3. Stecken Sie das Stromkabel in die vorgesehene Buchse
hinten am Gerät.
4. Stellen Sie das Gerät auf eine ebene Arbeitsfläche bzw.
(falls erforderlich) unter einen Laborabzug. Beachten Sie,
daß die Entlüftungsrippen an der Geräteunterseite immer
frei zugänglich sind.
5. Die Symbole auf oder neben dem EIN/AUS-Schalter an der
Geräterückseite bedeuten:
I
: An
O
: Aus
PAGE 6
Garantie
Die Garantiedauer des Gerätes ist auf der beiliegenden
Garantiekarte angegeben und schließt Fehler im Material oder
der Verarbeitung ein. Die Garantiedauer beginnt am Tag des
Einkaufs. Sämtliche defekte Teile werden innerhalb dieses
Zeitraumes kostenlos ersetzt unter der Voraussetzung, daß
dem Defekt keine unsachgemäße Handhabung, Fahrlässigkeit
oder ein Unfall zugrundeliegt. Der unter diese Garantie
fallende Service wird vom Lieferanten geleistet.
Ungeachtet der in dieser Gebrauchsanweisung enthaltenen
Beschreibungen und Spezifikationen, behält sich Techne
hiermit das Recht vor, Änderungen an den Geräten bzw. an
einzelnen Geräteteilen durchzuführen.
Diese Gebrauchsanleitung wurde ausschließlich dazu erstellt,
um Kunden die Handhabung der Techne-Geräte zu
erleichtern. Nichts in dieser Gebrauchsanleitung darf als
Garantie, Bedingung oder Voraussetzung verstanden werden,
sei es die Beschreibung, Marktgängigkeit, Zweckdienlichkeit
oder sonstiges bezüglich der Geräte oder deren Bestandteile.
Wartung durch den Bediener
BEACHTEN SIE, DASS DIESES GERÄT NUR VON
TECHNISCHEN FACHKRÄFTEN GEÖFFNET UND
DEMONTIERT WERDEN DARF.
DURCH ENTFERNEN DES GEHÄUSES ODER
GEHÄUSETEILEN SIND BAUTEILE MIT
LEBENGEFÄHRLICHEN SPANNUNGEN FREI
ZUGÄNGLICH.
IM INNERN DES GERÄTES BEFINDEN SICH KEINE TEILE,
DIE VOM ANWENDER GEWARTET WERDEN MÜSSEN.
Falls Ihr Gerät nicht ordnungsgemäß arbeitet, wenden Sie
sich an Ihren Lieferanten oder senden Sie das Gerät wenn
nötig zurück. Fügen Sie eine genaue Beschreibung des
Defektes bei. Verpacken Sie das Gerät möglichst im
Originalkarton. Bitte beachten Sie, daß Techne keine Haftung
bei Transportschäden aufgrund unzureichender Verpackung
übernnehmen. Setzen Sie sich im Zweifelsfall mit Ihrem
Lieferanten in Verbindung. Bitte beachten Sie die
Entgiftungsbescheinigung, die Sie mit dem Gerät erhalten
haben.
1. Reinigen
Bevor Sie Ihr Gerät reinigen, sollten Sie
• zuerst den Netzstecker ziehen
• das Gerät unter 50°C abkühlen lassen.
Ein feuchtes Tuch mit Seifenlösung reinigt Ihr Gerät am
besten. Achten Sie darauf, daß kein Wasser in das Gerät
gelangt. Verwenden Sie keine Scheuermittel.
2. Sicherungen
Die Stromzuleitung ist durch ein oder zwei Sicherungen
geschützt. Diese sollten nur durch qualifiziertes
Fachpersonal ausgetauscht werden. Wenn die Sicherung
wiederholt durchbrennt, liegt ein größerer Defekt vor. Das
Gerät muß zur Reparatur an Ihren Lieferanten eingesandt
werden.
TC-512
OPERATOR’S
MANUAL
Introducción
Después de su uso
Le rogamos lea cuidadosamente la información contenida en
este folleto antes de manipular el aparato.
Cuando haya finalizado el calentamiento de muestras,
recuerde que las piezas del equipo, tales como tubos, bloques
y demás accesorios, pueden estar muy calientes. Tome las
precauciones mencionadas anteriormente.
Aviso
LAS TEMPERATURAS ELEVADAS SON
PELIGROSAS: pueden causarle graves
quemaduras y provocar fuego en materiales
combustibles.
Techne ha puesto gran cuidado en el diseño de
estos aparatos para proteger al usuario de cualquier peligro;
aún así se deberá prestar atención a los siguientes puntos:
• EXTREME LAS PRECAUCIONES Y UTILICE GUANTES
PARA PROTEGERSE LAS MANOS;
• NO coloque objetos calientes encima o cerca de objetos
combustibles;
• NO maneje el aparato cerca de líquidos inflamables o
gases;
• NO introduzca ningún líquido directamente en el aparato;
• UTILICE EL SENTIDO COMUN en todo momento.
Seguridad del usuario
Todos los usuarios de equipos Techne deben disponer de la
información necesaria para asegurar su seguridad.
De acuerdo con las instrucciones contenidas en este manual
y con las normas y procedimientos generales de seguridad,
es muy importante que sólo personal debidamente capacitado
opere estos aparatos. De no ser así, la protección que el
equipo le proporciona al usuario puede verse reducida.
Todos los equipos Techne han sido diseñados para cumplir
con los requisitos internacionales de seguridad y traen
incorporados un sistema de desconexión en caso de
sobretemperatura. En algunos modelos el sistema de
desconexión es variable, lo que le permite elegir la
temperatura según sus necesidades. En otros, el sistema de
desconexión viene ya ajustado para evitar daños en el equipo.
En caso de que surgiera un problema de seguridad,
desconecte el equipo de la red.
Instalación
1. Todos los aparatos Techne se suministran con un cable de
alimentación. Puede ser fijo o independiente del aparato.
2. Antes de conectarlo, compruebe que el voltaje corresponde
al de la placa indicadora. Conecte el cable de alimentación
a un enchufe adecuado según la tabla expuesta a
continuación. El equipo debe estar conectado a tierra para
garantizar la seguridad eléctrica.
Conexiones
220/240V
110/120V
Linea
Marrón
Negro
Neutro
Azul
Blanco
Tierra
Verde/amarillo Verde
El fusible una vez instalado protege tanto al equipo como al
usuario.
Asegúrese de que los equipos marcados 230V en la placa
indicadora funcionan a 220V y de que los equipos
marcados 120V funcionan a 110V. No obstante, en ambos
casos la velocidad de calentamiento se verá reducida en un
8% aproximadamente. La placa indicadora está situada en
la parte posterior del equipo.
3. Conecte el cable a la toma de tensión en la parte posterior
del equipo.
4. Sitúe el aparato en un lugar apropiado tal como una
superficie de trabajo plana, o si fuera necesario incluso en
una campana con extractor de humos, asegurándose de
que las entradas de aire en la parte inferior no queden
obstruidas.
5. Los símbolos que se encuentran en o cerca del interruptor
de alimentación tienen los siguientes significados:
I
: Interruptor principal encendido
O : Interruptor principal apagado
Garantía
Este aparato está garantizado contra cualquier defecto
material o de fabricación durante el periodo especificado en la
tarjeta de garantía adjunta. Este plazo inicia a partir de la
fecha de compra, y dentro de este periodo todas las piezas
defectuosas serán reemplazadas gratuitamente siempre que
el defecto no sea resultado de un uso incorrecto, accidente o
negligencia. Mientras se encuentre bajo garantía las
revisiones las debe llevar a cabo el proveedor.
A pesar de la descripción y las especificaciones de los
aparatos contenidas en el Manual del Usuario, Techne se
reserva por medio de este documento el derecho a efectuar
los cambios que estime oportunos tanto en los aparatos como
en cualquier componente de los mismos.
Este manual ha sido preparado exclusivamente para los
clientes de Techne y nada de lo especificado en este folleto
de instrucciones se tomará como una garantía, condición o
aseveración de la descripción, comerciabilidad o adecuación
para cualquier fin específico de los aparatos o sus
componentes.
Mantenimiento
ESTE APARATO DEBE SER DESMONTADO SOLO Y
EXCLUSIVAMENTE POR PERSONAL DEBIDAMENTE
CAPACITADO.
EL RETIRAR LOS PANELES LATERALES, FRONTALES O
TRASEROS SUPONE DEJAR AL DESCUBIERTO TENSION
DE LA RED PELIGROSA.
EL EQUIPO NO CONSTA DE NINGUNA PIEZA DE CUYO
MANTENIMIENTO SE PUEDA ENCARGAR EL USUARIO.
En el caso improbable de que experimentara algún problema
con su aparato que no pudiera resolver con facilidad, debería
ponerse en contacto con su proveedor y devolverlo si fuera
necesario. Indique de forma detallada todos los defectos que
haya notado y devuelva el equipo en su embalaje original.
Techne no aceptará responsabilidad alguna por daños
causados en equipos que no estuvieran debidamente
embalados para su envío; si tuviera alguna duda, póngase en
contacto con su proveedor. Sírvase consultar el Certificado de
Descontaminación suministrado con su aparato.
1. Limpieza
Antes de limpiar su aparato, desconéctelo SIEMPRE de la
fuente de alimentación y permita que se enfríe por debajo
de los 50°C.
Este aparato se puede limpiar pasándole un paño húmedo
enjabonado. Hágalo con cuidado parae evitar que caiga
agua dentro del mismo. No utilice limpiadores abrasivos.
2. Fusibles
Su aparato está protegido por uno o dos fusibles. Sólo
deben cambiarlos personal debidamente capacitado.
Si los fusibles se fundieran repetidamente, esto indicaría
una avería grave y puede que tuviera que devolverle el
aparato a su proveedor para su reparación.
PAGE 7
TC-512
OPERATOR’S
MANUAL
The TC-512
Before using the TC-512 make sure you have read this manual carefully. If
there is any doubt relating to the proper use of this equipment, the staff at
Techne or your supplier will be pleased to assist you.
The TC-512 provides the researcher with the means of accurately controlling the
temperature profile of samples. It has many scientific applications, including DNA
amplification and sequencing. The TC-512 can cycle samples between 4°C
(39°F) and 99°C (210°F).
The TC-512 is programmed by means of a touch sensitive LCD graphics screen.
Operator input temperatures are temperatures which you require the sample to
achieve. Displayed temperatures can be the temperatures measured by the
block probe or the calculated sample temperature or both. Profile data is saved
in plug in memory cards, the program information is duplicated with the profile
data log, and so the original programs are not required during analysis. A limited
number of programs and all system settings are stored in nonvolatile memory
within the TC-512. Additional programs may be stored in the nonvolatile memory
cards, which are plugged into a slot under the cover above the screen.
A programme, which can be recalled from memory, consists of:
• a series of specified temperatures in °C
• the times for which each specified temperature will be held (Hold Times)
• the desired heating or cooling rates, in °C/min, between each specified
temperature
• whether the times and/or the temperatures are to increase or decrease when
a programme is repeated.
The memory card can store up to 500 three segment programs; the number of
programs is reduced as the number of segments in each program is increased.
The maximum is one program of 1500 segments.
PAGE 8
TC-512
OPERATOR’S
MANUAL
Warning !
Please note that the touch screen on the TC-512 is easily damaged by sharp
objects such as pens, pencils and fingernails. Damage of this sort will be
considered as misuse and will invalidate the guarantee on this component.
Position the unit so that the mains on/off switch is accessable. If a safety
problem should be encountered, switch off at the mains socket and remove the
plug from the supply.
If you are using more than one unit in close proximity to each other, there must
be at least 100mm between the units to allow the cooling air to flow from them.
Unpacking
When unpacking the unit, check that the following have been removed from the
packing:
• The TC-512 unit with memory card in place
• Mains cable •
Guarantee card
•
Decontamination Certificate
Keep the original packing, in case you ever need to return it for service or repair.
Techne accepts no responsibility for damage incurred unless the unit is correctly
packed and transported in its original packing.
Typical specification at an ambient of 20°C
For a detailed specification please contact Techne Sales
Temperature
TC-512 Range
20°C to 70°C
Maximum TC-512
30°C
Minimum TC-512
1°C
Number of temperature probes
4
Block temperature range
4°C to 99°C
Typical heat up rate
3.0°C/sec
Typical Cool down rate
1.3°C/sec
Block uniformity (without TC-512)
< ±0.4°C
Column uniformity with a 10°C TC-512
< ±0.3°C
Control accuracy
< ±0.3°C
TC-512 accuracy
< ±0.3°C
Temperature set point adjustment
±0.1°C
Integral Heated Lid
The heated lid only comes on if the block temperature is set above 30°C
Selectable heated lid temperature
100°C to 115°C
Heated lid enable/disable
Yes
Warm up time from ambient to 105°C
4 min
Over temperature cutout
Yes
Regulated lid pressure
Yes
Programs
Password protected
Yes
Incremental/decremental Segment Hold Time
-99 to +99sec, 1sec intervals
Incremental/decremental Temperature
-5.5°C to +5.6°C in 0.1°C intervals
Variable programmable ramp rate steps
±0.1°C/sec
Programmable ramp rate resolution
0.1°C
TC-512 calculator
Yes
Maximum programs internal memory
84 (dependant upon program complexity)
Maximum number of segments per loop
More than 60
PAGE 9
TC-512
OPERATOR’S
MANUAL
Maximum number of loops per program
Maximum programs memory card
Program naming capacity
Program interface screen
Pause facility
End of program alarm (can be disabled)
Auto restart on power failure
Run end time calculation
Miscellaneous
30
500 x 3 segment
Alpha/numeric
Touch sensitive
Yes
Yes
Yes
Yes
Height x Width x Length
260 mm x 220 mm x 420 mm
Weight
12.5 kg
Power consumption
620W total including the heated lid
Parallel port
Yes
Serial Port RS232/RS485 adaptable
Yes
Connection to PC control program
Yes
Voltage
See page 14 for setting the voltage.
The units marked 100V will work at any voltage between 90V and 110V.
The units set to 120V will work at any voltage between 110V and 130V.
The units set to 230V will work at any voltage between 210V and 260V.
However, the performance may not meet the above specification at the
extremes of voltage.
Working conditions
The TC-512 is designed to work safely under the following conditions:
Ambient temperature range
5°C to 40°C
Humidity
Up to 95% relative humidity, non-condensing
Note:The control specifications are quoted at an ambient temperature of 20°C.
The specification may deteriorate outside an ambient temperature range of 10°C
to 30°C.
Radio frequency interference tested and passed to EN50081-1.
Immunity Tested and passed to EN50082-1
Uses of the TC-512
The TC-512 has many scientific laboratory applications, including DNA
sequencing and PCR. Aspects of the PCR process are claimed in U.S. Patent
Nos. 4,683,195, 4,683,202, and 4,965,188. Use of the TC-512 in such
processes does not convey a licence to practice the processes themselves.
Tubes or reaction vessel
Techne does not recommend any specific tube or reaction vessel other than
those described in this Manual. We recommend using reaction volumes
between 10 and 50µl. The tubes must withstand a pressure of 1 atmosphere at
100°C. Any vessel must be able to withstand the temperatures you are using
without any danger of them deforming to the point where they fracture.
To test your tubes, put 25µl of water in each of 5 tubes and subject them to a
typical thermal cycling protocol. At the end of the cycle, measure the volume
remaining, using a micropipet. A loss of more than 1 or 2µl indicates a vapour
leak.
The amount of volume loss you observe and the change in reactant
concentrations you can tolerate determine the minimum volume that can be
used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 10µl
or less.
PAGE 10
TC-512
OPERATOR’S
MANUAL
During the final cooldown, a ring of condensation may form above the liquid level
but below the top of the sample block. This is not usually a cause for concern,
as the condensation does not form during cycling.
Cleaning your TC-512
Disconnect your unit from the power supply and allow it to cool to below 50°C
before cleaning it.
The heat/cool block, including wells and flat surfaces, should be cleaned
regularly to ensure optimum heat transfer to the samples. Always clean the
block if there has been a spillage. Use a cloth or cotton buds dipped in a fresh,
50:50 water/isopropanol solution, and make sure that no deposits are left in the
wells.
In the case of radioactive spillages, Techne recommend that you use a
proprietary cleaning agent. Carefully follow the cleaning agent manufacturer’s
instructions. The heat/cool block is made of aluminium. Therefore, an agent
such as Neutracon (from Decon Laboratories Ltd.), suitable for nonferrous
metals should be used but remember other parts of the unit are made of ferrous
materials and may be damaged by spillage onto them.
Before using any cleaning or decontamination method except those
recommended here, the responsible body should check with Techne that the
proposed method will not damage the equipment.
You can clean the case or the screen of the TC-512 with a cloth dipped in water
or ethanol (methanol or formaldehyde can also be used). No part of the case or
cover should be immersed in the solvents. Do not use aggressive solvents such
as acetone, or abrasive cleaners.
Block removal
The blocks can be removed for a
more detailed cleaning.
Ensure that the unit is disconnected
from the mains for removal of block.
Slide the quick release handles in
the direction shown above and lift
the assembly out of the unit.
Never lift or carry the block by one
quick release handle, always use
both handles or support the block
underneath. When refitting the
block, slide the quick release
handles in the opposite direction
to the above arrows to lock the
block assembly into the unit.
PAGE 11
TC-512
OPERATOR’S
MANUAL
The heated lid
The heated lid is an integral part of the TC-512. It can be switched “On” or “Off”,
see ‘Program Defaults’ or ‘Editing screen’.
To open the lid lift the back of the handle as shown and use it to lift the lid. To
close the lid lower the lid onto the block with the samples in and press the handle
shut.
The rubber pad for inside the lid is loose so that it can be replaced easily or used
when appropriate. The part number of the loose mat is 6104978.
The rubber mat supplied with the unit is for use with microplates when sealing
using a plastic adhesive film. The rubber mat should be placed on top of the
sealing film to ensure good thermal contact with the heated lid.
To adjust the pressure on the samples:
The heater plate height should only be
adjusted with the lid in the down
position, as shown, but not necessarily
latched.
Adjust the orange rotating knob
anticlockwise to give minimal
pressure, shut the lid and latch
it. Gently rotate the knob
clockwise until you can just feel
pressure beginning to be
applied. Rotate the knob a
further quarter of a turn and
this will give the correct
pressure. Do not over-tighten
the knob as this will cause
deformation of the silicone
rubber membrane and reduce the
effectiveness of the lid. Once the pressure
has been set it does not need to be
adjusted unless a different block, and
hence a different type of sample holder, is
used.
PAGE 12
TC-512
OPERATOR’S
MANUAL
The memory card
The Memory Card is inserted into the slot
under the cover at the front of the unit. Lift
the cover and push the Memory Card into
the slot until the button at the left-hand end
of the slot rises. Ensure that the Memory
Card is inserted correctly by positioning the
card so that the ‘front’ label is facing you.
The Memory Card can be used for storing
the programs and data logs of program
runs. The memory card slot also allows
upgrades of the control software. Either
via copying software from a new unit and
transferring it to an older unit, or by
obtaining a copy of the new software from
Techne This is a relatively easy process,
see the “System Administration” card, and
means that you can keep your unit up to
date with the latest software.
Adjusting the light Intensity of the screen
The light intensity of the screen can be
adjusted to suit various environmental
conditions. Lift the front cover and you will
see a small black knob on the left-hand
side of the cavity. Rotating this knob
clockwise will make the screen lighter and
rotating it anticlockwise will make the
screen darker. Adjust the screen to suit
your light conditions.
Adjusting Knob
PAGE 13
TC-512
OPERATOR’S
MANUAL
Before switching on
Ensure that the voltage selector switch is set to the appropriate voltage for your
supply.
Connections on the back of the unit
For 230V set the
voltage selector
switch set to
230V and fit
T6.3A fuses.
Selector switch
RS485 (Multiple
units) or RS232
(Single unit)
For 115V set the
switch to 115V
and fit T8A
fuses.
Serial port
Parallel port
RS485
connection (see
next page)
Selector switch:
“Daisy chain”
(any unit except
last in chain);
“Termination
Box” (last unit in
chain).
Symbols on the screen
is the symbol for setting the gradient
PAGE 14
230
For 100V the
switch will be set
to 115V T10A
fuses will be
fitted.
On/Off switch
Ensure that the
appropriate
fuses are fitted
for your supply.
TC-512
OPERATOR’S
MANUAL
Connecting one unit to the computer using an RS 232 cable
If you are driving one unit only you will need FGEN232which includes; a cable,
the software on disc and an instruction sheet.
Connecting several units to the computer using the daisy chain cables
If you are driving two or more units then you will need FTG485D an RS485 pack
set. A pack set includes: the connection to a computer which also converts to
RS485; a 1 metre cables and the software on disc with an instruction sheet.
You will also need one of the following for each unit in the ‘Daisy Chain’:
6103557
Connecting cable.
Notice from the diagram that the cable connections to the Cycler's are
alternately bottom to top on the connector.
TC-512
PAGE 15
TC-512
OPERATOR’S
MANUAL
Some frequently asked questions
Q1
A1
Q2
A2
Q3
A3
Q4
A4
Q5
A5
Q6
A6
Q7
A7
PAGE 16
What’s the ‘pause function’ (as far as it concerns the before program
operation)?
Some users prefer to preheat the heated lid before placing the samples
into the unit. The pause feature is used to stop the unit after the 4 minute
heated lid preheat step. It will also sound an audible alarm indicating that
the the machine is ready for the sample tubes or plate to be added.
Pressing ‘unpause’ twice will commence the remaining program.
Why do you have to press some keys twice to make the unit do what you
want it to do.?
With many of the program features there is a fail-safe function which asks
if you are sure you want to perform a particular action. You then have to
press a key a second time.
What is the appropriate pressure of the heated lid? If we do it the way it is
described in the manual the tubes are stuck on the membrane.
If the tubes stick to the lid then this could be because there is some grease
or dirt on the rubber pad. We recommend that the user occasionally dusts
the rubber pad with talcum powder to avoid sticking. It is important not to
overtighten the lid a light contact is sufficient.
What is “Hot start”?
Hot Start is used to make the machine pause after the initial denaturation
at a specific temperature, typically around 70’C. The reason is to allow the
addition of TAQ enzyme after the initial 5min denaturation, this prolongs
the half-life and activity of the enzyme.
What is the incremental function? See pages 30 and 31.
Incremental timing and temperature are used to increase either the time or
temperature incrementally over the 30 cycles.
Incrementation of time is used with ‘Long PCR’ which is when large
template fragments are to be amplified (27KB lambda, 40KB genomic).
Decremental temperature is used for protocols such as ‘Touchdown PCR’
where one starts at a high anneal temperature and gradually decrease the
temperature over the 30 cycles, this ensures a highly specific product is
amplified.
The way you enter a new programm is not quite understandable.
The logic of this manual is as follows:
a Open programs
1 New programs
2 Templates
3 Existing programs
b Edit programs (including adding segments, loops, etc, to a new
program)
c Run programs
Once you are doing what the heading says you remain in that function until
you get to another heading.
It is not always clear the order that you add parts to the program.
The software will always add a line if you have missed it out. For example
if you add a ‘segment’ and you have not added the ‘cycles’ the software
will add a line ‘Number of Cycles 1’ above the segment. You can then
edit this line if you require more than one cycle.
If you require further assistance please telephone Techne with the unit in
front of you and we can go through the programming function.
TC-512
OPERATOR’S
MANUAL
Power up screens
Techne
TC-512
Serial number
GPxxxx/xx
Software version
v.vv
Hardware version
h.hh
Block ID
Grad 96 x 0.2ml
Last system test
dd/mm/yyyy
Copyright protected 2000
The screen shown is
displayed when the unit
is powered on and will
remain displayed while
the unit performs it’s
self tests. After the
self-tests this screen is
displayed unless the
unit is powered up by
auto-restart see a later
section.
Performing self tests
1
2
3
An example of a ‘field’
with full corners
which are dialogue
windows and have no
effect when touched.
Techne
TC-512
An example of a ‘field’
with cutaway corners
which are touch
sensitive and give the
user some sort of
action when touched.
System
User
Techne
TC-512
System User button
Serial number
GPxxxx/xx
Software version
v.vv
Hardware version
h.hh
Block ID
Grad 96 x 0.2ml
Last system test
dd/mm/yyyy
Comms address
01
Copyright protected 2000
System
User
The screen is a touch
sensitive LCD graphics
screen. Operator input
temperatures are
temperatures which you
require the sample to
achieve. Displayed
temperatures are either
the temperatures
measured by the block
probe, the calculated
sample temperature or
both.
System
Admin
This selects normal
user functions as shown
on the next screen.
System Admin button
This selects
administration functions
and is dealt with in the
system administration
section.
PAGE 17
TC-512
OPERATOR’S
MANUAL
System user main menu
Techne
TC-512
Programs
Data
Logs
Gradient
Calculator
Program
defaults
Exit
Programs
By touching this button you
can access all the programs
stored either internally or in
the memory module, see
page 20.
Program defaults
By touching this button you
can set up the program
defaults for a number of
programs. See below.
Data Logs
By touching this button you
can access the data logs
stored in the plug in memory
module. See a later section
of this manual, pages 34
and 35.
Gradient Calculator
See page 28.
Exit
By touching this button you
can return to the previous
screen.
Program defaults
Pause before program
Off
One touch gives:
Pause before program
On
A second touch will return the field to the first example.
Set program Defaults
Heated Lid
Off
Heated Lid before program
Off
Pause before program
Off
Initial denaturation 05m00s
94.0°c
Hot Start
94.0°c
▼
▲
Exit
PAGE 18
When you touch this
button you can set up
default settings for the
programs you are about to
put into the unit.
First is an example where
if you touch the button for
a field where there is either
ON or OFF then the field
will toggle between the
two.
If you touch a button
where there is a numerical
input it will transfer you to
an input screen. An
example is given on the
following pages.
If you touch the ’▼’ down
arrow button the screen
will toggle down to a
maximum of four more
fields as shown on the
next screen. You can then
toggle back using the ’▲’
up arrow button.
TC-512
OPERATOR’S
MANUAL
Set program Defaults
Initial denaturation
05m00s 94.0°c
Hot Start
94.0°c
Final extension
05m00s
Final hold
▼
72.0°c
10.0°c
Once you have set the
defaults to those required,
you return to the “System
User Main Menu” screen
by touching the Exit
button.
▲
either
Exit
or
Numerical Input Screen
An example only is shown. All the screens where a numerical input is required
are similar. The ‘h’, ‘m’ and ‘s’ will only appear where the field requires hours,
minutes or seconds.
The ’← →’ arrow buttons only operate where you need to change fields.
The ‘Disable’ button puts ‘xxxxx’ in the fields. The button itself will change to
‘Enable’. If the segment was previously “Disabled” the reverse will happen and
you will be able to put numerical values in the field.
The ‘Cancel’ button takes you back to the previous screen without any changes.
The ‘BS’ button is a back space, if you are at the beginning of a field it will delete
the whole field: if you are in the middle of a field it will delete the previous digit.
Type in the program information; with the units of time where appropriate. The
order of the time is not important, for example if you type in 5s2m the unit will
convert it to 02m05s.
Edit initial denaturation settings
Initial denaturation
95.0°C 05m00s
h
7
8
9
←
→
m
4
5
6
Disable
s
1
2
3
Cancel
0
.
BS
OK
Touch ‘OK’ when you have
entered the your program
information.
You will return to the
“Program” screen.
PAGE 19
TC-512
OPERATOR’S
MANUAL
Programs
b
b
b
b
i
▼
Touch the ‘Programs’
button in “System User
Select program
Main Menu” and you will
xxxxxx bytes of memory free
go into the “Select
Program” screen.
2 STEP PCR TEMPLATE
How to use “New”
3 STEP PCR TEMPLATE
programs is explained
below.
ICE BUCKET
An “Existing” program with:
LIGATION 15 DEGREES
a “b” prefix is a ‘basic’
Bill
program or “Template”
(how to use these is
▲ either
explained on page 21);
an “i” prefix means the
New
Exit
program is stored
‘internally’;
or
an “r” prefix means the
program is stored on the memory card or ‘remotely’(how to edit and use existing
programs are explained on page 23).
Use the, ‘s’or ‘t’, down arrow or up arrow key to toggle through the programs.
Select any one by touching the appropriate field.
If you touch ‘Exit’ you will return to the System User Main Menu.
New program screen
If you choose ‘New’ then you will go into an input screen to give the program a
name. Once you have entered the name, you will go into the ‘Editing’ screen.
Touch the ‘t’ down arrow button to toggle more parts of the program. You can
then toggle back using the ‘s’ up arrow button.
‘Del(ete)’ will only be
effective for saved
programs.
Editing
‘Ins(ert)’ will only be
effective if there is no
“Next step” field.
“Your Own”
Initial denaturation
05m00s 94.0°c
Hot start
Disabled
Next step
Final extension
05m00s 72.0°c
10.0°c
Final hold
▼
▲
Ins
PWord
PAGE 20
Save As
Del
Edit
Save
Exit
‘Save As’ will give the
program a different name.
‘Save’ will save the
program.
‘Edit’ will put you into the
“Edit program function”
screen.
‘Exit’ will return you to the
“Program” screen.
‘PWord’ will allow you to
give the program a
password so that other
people cannot edit your
program, see page 24.
TC-512
OPERATOR’S
MANUAL
Template program screens
Program
2 STEP PCR TEMPLATE
Last Modified
01/00/2000 00:00:00
105.0°c
Heated Lid
Heated Lid before program
On
Pause before program
Off
Initial denaturation
05m00s 94.0°c
Hot Start
Disabled
Number Cycles
▼
30
▲
Copy
Delete
Edit
Print
Exit
Run
Program
2 STEP PCR TEMPLATE
05m00s 94.0°c
Initial denaturation
Hot Start
Disabled
Number Cycles
30
Seg
MAX°c/m
00m30s
92.0°c
Seg
MAX°c/m
01m00s
68.0°c
Final extension
05m00s 72.0°c
10.0°c
Final Hold
▼
▲
Copy
Run
Delete
Edit
Print
Exit
Any template program can
be copied and given a new
name.
From “Select Program”
screen if you touch “2 step
PCR template” you will
come to this screen.
Touch ‘Copy’ and you will
go into the title screen.
Type in your name for the
program and touch ‘Enter’.
If you try to ‘Edit’ any of
these programs you will
find that you can only
“Save as” when you finish.
The ‘Save’ key will not be
on the screen.
If you touch ‘Delete’ you
will be asked if you are
sure you want to delete the
program. Select ‘Yes’ or
‘No’.
Before you touch ‘Print’
make sure that you have a
printer connected to the
TC-512. If you touch
‘Print’, and you have a
printer connected, it will
print out the program.
If you touch ‘Run’ the unit
will start to run the
selected program. See a
later section on page 32.
The other keys work as for
any other screen.
Touch the ‘▼’ down arrow
button to toggle more parts
of the program.
You can then toggle back
using the ‘▲’ up arrow
button.
PAGE 21
TC-512
OPERATOR’S
MANUAL
Program
3 STEP PCR TEMPLATE
Hot Start
Disabled
Number Cycles
30
Seg
MAX°c/m
00m30s
92.0°c
Seg
MAX°c/m
00m30s
55.0°c
Seg
MAX°c/m
00m30s
72.0°c
05m00s 72.0°c
Final extension
10.0°c
Final Hold
▲
▼
Copy
Delete
Edit
Print
Exit
Run
Program
ICE BUCKET
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
Disabled
Final extension
Disabled
▼
Program
LIGATION 15 DEGREES
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
Disabled
Final extension
Disabled
Final hold
▲
PAGE 22
The “Ice Bucket” template
has all fields set to ‘off’ or
‘disabled’ except the final
hold temperature which is
set to 4.0°C.
4.0°c
Final hold
▲
The 3 step PCR template
has the seg(ments) shown
here. Other settings are
as for the 2 step template.
▼
15.0°c
The “Ligation 15 degrees”
template has all fields set
to ‘off’ or ‘disabled’ except
the final hold temperature
which is set to 15.0°C.
TC-512
OPERATOR’S
MANUAL
Existing program screen
Program
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
▲
94.0°C
Disabled
▼
Copy
Run
Program
Delete
Edit
Print
Exit
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
▲
Disabled
▼
Copy
Run
94.0°C
If you select an existing
program from the
“Programs” screen you will
get a screen similar to this
one.
Touch the ‘▼’ down arrow
button to toggle more parts
of the program. You can
then toggle back using the
‘▲’ up arrow button.
If you touch ‘Delete’ you
will be asked if you are
sure you want to delete the
program. Select ‘Yes’ or
‘No’.
Before you touch ‘Print’
make sure that you have a
printer connected to the
TC-512. If you touch
‘Print’, and you have a
printer connected, it will
print out the program.
If you touch ‘Run’ the unit
will start to run the
selected program. See a
later section on page 32.
In order to edit the
program you must touch
the ‘Edit’ button to get into
the “Editing” screen. For
editing see the following
pages.
‘Exit’ will return you to the
“System User Main Menu”.
Delete
Edit
Print
Exit
PAGE 23
TC-512
OPERATOR’S
MANUAL
Editing programs
Program
Bill
Last Modified 19/08/2000 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
94.0°c
05m00s
Number Cycles
▼
3
▲
Copy
Delete
Edit
Print
Exit
Run
From the “Program”
screen touching ‘Edit’ puts
you into a very similar
screen. The top field will
have gone and the next
field will have appeared at
the bottom of the set of
seven fields, as shown in
this picture.
Password protection
Editing
Bill
Initial denaturation
Hot Start
Disabled
05m00s
Number Cycles
72.0°c
3
Seg MAX°C/m
00m30s
92.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
▼
▲
Ins
PWord
PAGE 24
Disabled
If you do not want other
people editing the program
it may be password
protected using ‘PWord’.
You will need to enter it
twice to confirm the
password.
Next time you go into
“Editing”, the ‘PWord’ key
will have changed to
Touch any field you need
to edit and it will become
highlighted.
Save As
Del
Edit
Save
Exit
Change
PWord
TC-512
Editing
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
94.0°c
05m00s
Number Cycles
Seg MAX°C/m
00m30s
3
92.0°c
10°c
Switch a function on or
off
If the field has “On” or
“Off”, the field will toggle
between “Off” and “On”
when you touch the ‘Edit’
button with the field
highlighted.
▲
Ins
PWord
Save As
Editing
Del
Edit
Save
Exit
Bill
Initial denaturation
Hot Start
Disabled
05m00s
either
72.0°c
Number Cycles
3
Seg MAX°C/m
or
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
▼
MANUAL
Bill
Heated Lid
▼
OPERATOR’S
Disabled
Editing Numerical Values
If the field has a numerical
value or says “Disabled”,
you will get a “Numerical
Input” screen when you
touch the ‘Edit’ button with
the field highlighted.
▲
PWord
Ins
Del
Edit
Save As
Save
then
Exit
PAGE 25
TC-512
OPERATOR’S
MANUAL
Editing
Bill
Initial denaturation
Disabled
Hot Start
72.0°c
05m00s
Number Cycles
3
Seg MAX°C/m
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
10°c
Next step
Final extension
▼
Disabled
▲
Ins
PWord
Save As
Del
Edit
Save
Exit
Editing
Bill
Initial denaturation
Hot Start
Disabled
05m00s
Number Cycles
00m30s
55.0°c
Seg MAX°C/m
00m30s
92.0°c
Disabled
▲
Ins
PWord
PAGE 26
10°c
1
Final extension
▼
72.0°c
3
Seg MAX°C/m
Next step
3
Deleting a Segment
‘Del’ will delete any
segment which is
highlighted, but you will be
asked to confirm the
deletion.
2 Save As
Del
Edit
Save4
Exit
Inserting Extra Functions
1 Highlight the field after
which you wish to add
program information, for
example the second
“Seg(ment)” in the
illustration.
2 Touch ‘Ins(ert)’. A “Next
Step” will be added after
the highlighted field. (Or
you can use the “Next
step” that the program
inserts automatically.)
3 Touch the ‘Next Step’
field and highlight it.
4 Touch ‘Edit’. You will
now be in the “Edit
Program Function” screen.
TC-512
Next Step
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
→
Edit program function
Seg
75.0°c
0°c
00m30s
MAX°c/m
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
MANUAL
Segment
Touch ‘Segment’ to
insert a segment.
Edit program function
Pause
OPERATOR’S
→
Type in the Temperature
as appropriate.
Use the “Right Arrow”
→’ to move to the
key ’→
Gradient field.
Type in the Gradient as
appropriate. See page
28.
Use the “Right Arrow”
→’ to move to the
key ’→
Time field.
Type in the Hold Time as
appropriate.
Use the “Right Arrow”
→’ to move to the
key ’→
Ramp field.
Type in the Ramp Rate
as appropriate. The
Ramp rate you put in
here will be the rate from
the temperature at the
begining of the
“Segment” to the
temperature of the midpoint of the “Gradient”.
Rates to the highest and
lowest temperatures will
be adjusted
appropriately.
PAGE 27
TC-512
OPERATOR’S
MANUAL
The gradient
The Set Temperature is the temperature that you require of the MIDDLE ROWS
of samples in the block. The GRADIENT is the variation of the two extremes of
the samples in the block; the left-hand side being the coolest and the right-hand
side being the hottest.
LHS
Centre of Block
RHS
Highest temperature
Gradient 10°C
Temperature
60°C
55°C
50°C
Set temperature
Lowest temperature
1
2
3 4 5 6 7 8 9 10 11 12
Columns
The Maximum gradient is 30°C; the lowest cold temperature is 20°C and the
hottest temperature is 70°C. However, you must remember that the greater the
gradient the poorer the uniformity.
The illustration shows a
Set Temperature of 55°C with a Gradient of 10°C on a 0.5ml x 60 block.
Other examples: Set temperature of 55°C with a gradient of 20°C will give a low
of 45°C and a high of 65°C; a set temperatureof 50°C with a gradient of 16°C will
give a low of 42°C and a high of 58°C.
Gradient Calculator
While the gradient is
Gradient Calculator
substantially linear there
are small variations. We
Temperature 55°c
Gradient 10°c
have, therefore, included a
gradient calculator to give
you a more accurate
picture of the gradient.
Touch the ‘Gradient
Calculator’ button in
“System User Main Menu”,
see page 18, and you
50.6
52.0
53.7
55.2
57.2
58.8
will go into the “Gradient
51.2
52.9
54.6
56.2
58.0
59.2
Calculator” screen.
Gradient
An example is shown here
Print
Exit
but please note that this is
Temperature
an idealised picture for this
manual and you must use
the unit screen to get the
accurate picture for your
block.
PAGE 28
TC-512
Next Step
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Continue
Inc
Dec
s
1
2
3
→
Cancel
0
.
BS
OK
First
Pause
MANUAL
If you want to add other
information, touch
‘Continue’ then the
appropriate key
Edit program function
Then
OPERATOR’S
Cycles and/or Pause
You can also insert
‘Number of Cycles’,
and/or a ‘Pause’ as
appropriate and adjust
the values to suit.
and/or
Edit program function
First
Last
75.0°c
75.0°c
0°c
0°c
00m30s
00m30s
MAX°c/m
MAX°c/m
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Disable
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
→
Inc/Dec time or
temperature
You can insert an
incremental value for
Time or Temperature
and these are illustrated
on the next page.
Touch ‘Inc/Dec’.
PAGE 29
TC-512
OPERATOR’S
MANUAL
To Program increment or decrement temperature or time.
Under normal circumstances, the Hold Temperature of all segments is constant.
However, it is possible to automatically increment or decrement the temperature
of a specified segment of a programme.
The Hold Temperature of the incremented/decremented segment is the defined
Hold Temperature plus the summation of the increments/decrements. The first
cycle is never incremented/decremented, only subsequent Cycles. If you select
a decrement, the Hold Temperature is prevented from falling below 4°C. If you
select an increment, the Hold Temperature is prevented from rising above 99°C.
Similarly, under normal circumstances, the Hold Time of all segments is
constant. However, it is possible to automatically increment or decrement the
duration of a specified segment of a programme.
The Hold Time of the incremented/decremented segment is the defined Hold
Time plus the summation of the increments/decrements. The first cycle is never
incremented/decremented, only subsequent Cycles. If you select a decrement,
the Hold Time is prevented from falling below one second.
PAGE 30
TC-512
OPERATOR’S
MANUAL
Edit program function
Next Step
Segment
h
7
8
9
←
Number
Cycles
m
4
5
6
Disable
Inc
Dec
s
1
2
3
Cancel
0
.
BS
OK
Pause
→
Completion
Touch ‘OK’ when you have
the values you need in
each of the fields. You will
return to the “Editing”
screen.
‘Save’ before ‘Run’
Editing
“Your own”
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Number Cycles
Seg MAX°c/m
▼
72.0°c
3
00m35s
PWord
Save As
Alternatively you can give
the program a different
name by touching ‘Save
As’ and following the on
screen instructions.
No Change
75.0°c
0°c
▲
Ins
Once you are satisfied that
the information is correct
you must “Save” the
program. Touch ‘Save’.
Del
Edit
Save
Exit
Use ‘Exit’ to return to the
“Program” screen without
editing any information. If
you have made changes it
will ask you if you want to
save them.
PAGE 31
TC-512
OPERATOR’S
MANUAL
Running a program
Program
“Your own”
Last Modified 19/08/98 14:16:43
Heated Lid
Disabled
Heated Lid before program
Off
Pause before program
Off
Initial denaturation
Disabled
Hot Start
05m00s
Final extension
▼
72.0°C
Disabled
▲
Copy
Run
either
Delete
Edit
Print
Exit
or
From the “Select program”
screen you will go into the
“Program” screen for the
program selected.
Alternatively, from the
“Editing” screen you will
go back into the “Program”
screen when you ‘Save’ or
‘Save as’.
From the “Program”
screen when you touch
‘Run’ you will be asked if
you wish to data log the
run.
If you answer ‘Yes’ then
you will be asked to give
the data log a name. For
printing out the data log
and so on see a later
section on page 34.
Typical Program Printout
From the “Program” screen
you can print the program
by touching the ‘Print’ key.
A typical print-out is shown
below.
TC-512 program
02/12/98 08:29:44
System Version
TC-512 Serial Number
Program
Date last edited
:0.49 1.00
: 88192/01
: DEPT PROGRAM
: 12/10/1998 22:48:11
Auto Restart
Password Protection
Heated Lid
Initial Denaturation
Hot Start
: Enabled
: Disabled
: Enabled
: Disabled
: Disabled
01 Cycles
Segment
03 Cycles
Segment
Segment
Segment
PAGE 32
Set Point°C
4.0
RampRate°C
Max
HoldTime
00:10:00
Set Point°C
95.0
55.0
72.0
RampRate°C
Max
Max
Max
HoldTime
00:00:30
00:00:30
00:01:00
TC-512
OPERATOR’S
MANUAL
While running programs
Program
“Your own”
Data log None
Cycle 1 of 6
step 5 of 8
Holding at 55.0°c
10.0°c
Sample Lw =
50.0°c, Sample Up
= 60.0°c
Time to complete 01m26s
100
50
0
00h01m
Stop
00h02m
00h03m
Pause
00h04m
00h05m
As the program
progresses you will
eventually get a screen
similar to this diagram.
The trace is plotted from
the right-hand side and so
initially you will have a
blank graph with two axis
lines. The trace will
consist of one or two lines,
the block
temperature, the sample
temperature or both, this
depends on the selection
you made in “Program
Defaults”.
If you wish to pause the
program touch the ‘Pause’
button. Press ‘Unpause’
and the program will
continue.
If you wish to stop the
program, you have to
press ‘Stop’ twice. This is
to prevent accidental
interruption of the
program. The fields at the
bottom of the screen will
change to ‘Run’ and ‘Exit’.
Touching ‘Run’ will start
the program from the
beginning again.
Touching ‘Exit’ will take
you back to the program
screen.
PAGE 33
TC-512
OPERATOR’S
MANUAL
Data logs
Select data log
i Bill
i Bob
i Dick
“Your own”
▼
▲
Print
data log
Delete
data log
Exit
Once a program has run
and the data has been
logged, it can be printed
on a Epsom dot-matrix
fan-form LX300+ printer.
Or it can be deleted. Both
actions are performed
from the “System User
Main Menu”. Touch ‘Data
logs’ and you will get the
screen shown here.
Select either ‘Print data
log’ or ‘Delete data log’.
If you select print ensure
that you first have a printer
connected.
‘Exit’ will return you to the
Main Menu.
Printing data logs
As the TC-512 runs, profile information is stored on the Memory Card. This
information defines the times and temperatures at various points during the run.
A range of events creates data which is used to create a profile of the run.
If you require a printed output give the data log a name when requested, the
data will then be stored under that name while the program is running. Once the
program is complete, from the “System User” screen choose ‘Data Logs’. Select
the Data Log that you want to print by highlighting it. Make sure that the printer
is plugged in and set on line before pressing the print key. Note that profiles
cannot be printed out during calibration.
Start
The events which are used to generate profile information are:
A start of program event is generated on first pressing the run key.
Stop
A subsequent press of the stop key stops a run prematurely. This event
generates a printer form feed.
Start of program
Whenever a new program starts. This event generates a Start of cycle
event.
Start of cycle
Whenever the current program starts a new cycle. This event generates
a Start of Segment event.
Start of segment
Whenever a new segment starts.
Start of Hold Time
Whenever the current segment reaches the Hold Time window.
End of program
Whenever the run ends due to the program finishing. This event
generates a printer form feed.
Pause on
Whenever the pause key is first pressed to pause a running program.
Pause off
PAGE 34
Whenever the unpause key is subsequently pressed to end a pause
period.
A printed profile is 80 columns wide and uses only standard characters in the
ASCII range 1 to 127: thus all common dot matrix, pin fed, continuous stationery
printers can be used.
TC-512
OPERATOR’S
MANUAL
An example of a typical printed profile is shown here:
Note the following:
• The second line shows the block type detected and the software version
number.
• At the start of each cycle, the cycle number is shown.
• The start time is the time according to the real time clock in the unit.
• The Rate is prefixed with either a ‘+’ or ‘-’ to indicate a heating (+) or
cooling (-) segment.
Typical Data Log Printout
TC-512 program
30/11/98
System Configuration.
System Version
:
Unit Serial No.
:
Last System test
:
Program
Date last edited
Data Log File
:
:
:
Event
Date & Time
Program
Ramping
Holding
Ramping
Holding
Ramping
Holding
Ramping
Holding
Ramping
Holding
14:27:25
0.49 1.00
88192/01
19/11/1998 09:34:24
DEPT PROGRAM
12/10/1998 22:46:11
NORMAN
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
30/11/1998
14:27:26
14:27:26
14:27:51
14:37:52
14:38:29
14:38:59
14:39:26
14:39:56
14:40:05
14:41:05
14:41:16
Func
Cycle Step
Temp
01
01
02
02
02
02
02
02
02
02
01
01
01
01
01
01
01
01
02
02
4.0°C
6.3°C
4.0°C
88.1°C
95.0°C
59.2°C
55.0°C
65.5°C
72.0°C
88.5°C
01
01
01
01
02
02
03
03
01
01
Set
Point
Ramp
Rate
4.0°C
4.0°C
95.0°C
95.0°C
55.0°C
55.0°C
72.0°C
72.0°C
95.0°C
95.0°C
MAX °C/m
MAX °C/m
MAX °C/m
MAX °C/m
MAX °C/m
Signed:
Typical Graph from a Data Log
Data Log
:
Program Name :
Time Started :
0
NORMAN
DEPT PROGRAM
30/11/1998 14:27:26
10
20
Temperature (°C)
30
40
50
60
70
80
90
Time (m) 0
5
10
20
PAGE 35
TC-512
OPERATOR’S
MANUAL
Using Gensoft
The “Gensoft” software package is available to enable you to connect the
TC-512 to a computer via a ‘Comms’ port. “Gensoft” offers:
A Daisy chaining a number of units together to a maximum of 32 units
B Creation of thermal cycling protocols through as easy to use Microsoft
Windows user interface and transmitting them to one or more units.
C Selection of units to start and stop a program
D Diagrammatic status of all units connected, including information on the
current position in the protocol of each individual unit and the time remaining.
F Print out of the protocol in a diagrammatic format together with associated
notes page.
If you are driving one unit only you will need:
FGEN232
An RS232 cable set which includes; a cable, the software on disc
and an instruction sheet.
If you are driving two or more units then you will need:
FTGEN485
A Computer Connection set.
This set includes: the connection to a computer; the necessary cables; and the
software on disc with an instruction sheet. There are two versions of the
software, one which drives up to 5 units and is faster than the version that will
drive up to 32 units.
and the following:
6103557
A cable to connect the units together, one for each unit.
Contact your dealer or Techne Sales for a full list of parts required for your
application.
PAGE 36
TC-512
OPERATOR’S
MANUAL
Additional information
Brief fault finding notes and a list of replacement parts are given in this section.
Note that this equipment should only be dismantled by properly trained
personnel. Removing the outer cover exposes potentially lethal mains
voltages.
There are no user serviceable parts within this equipment.
Fault Finding
Should you have any problems with your TC-512 which cannot be easily
remedied, you should contact your supplier and return the unit if necessary.
Please include details of the fault observed and remember to return the unit in its
original packing. Techne accept no responsibility for damage to units which are
not properly packed for shipping: if in doubt, contact your supplier, giving the full
serial number of the unit and software version number (shown when the unit is
first switched on).
Fuses
If neither the power light nor displays on the front panel are lit, one of the two
fuses may have blown. Check that there is no external cause, such as a faulty
plug or lead. Check both fuses and replace the faulty fuse with a new one of the
correct value. (Fuse values are given on the label next to the power inlet.) Note
that fuses should only be replaced by a qualified electrician.
The holder for the two fuses is built into the mains input socket. First remove the
power cable, then gently prise the fuse drawer open with a flat-bladed
screwdriver or similar tool.
Each fuse can be removed by using the screwdriver as a lever.
Exchange the faulty fuse in the fuse holder for a working fuse of the correct
value. Finally, replace the fuse drawer in the fuse compartment and push the
drawer shut.
Fuses which blow repeatedly indicate a serious fault and you should return the
unit to your supplier for repair.
Insulation Testing
This equipment is fitted with RFI suppression circuitry. Any check of the
electrical insulation by means of high voltage dielectric testing (for example as in
BS EN 61010-1) must be carried out using only a DC voltage.
This unit contains semiconductor components which may be damaged by
electric field effects.
PAGE 37
TC-512
OPERATOR’S
MANUAL
Interchangeable blocks
Block removal
Ensure that the unit is turned off and
disconnected from mains.
The block can be removed and exchanged for
another as required.
Slide the quick release handles in the direction
shown above and lift the assembly out of the unit.
Never lift or carry the block by one quick release
handle, always use both handles or support the
block underneath.
When refitting the block, or fitting a new one, slide
the quick release handles in the opposite direction to
the above arrows to lock the block assembly into the
unit. The software will register the change and
calibrate the unit for the new block.
Remember to adjust the options in the Program
Defaults Menu for the new application.
If you have fitted the flat block for slide applications
you must choose Heated Lid OFF. See the section
headed Program Defaults Menu.
PAGE 38
TC-512
OPERATOR’S
MANUAL
Accessories
The following accessories can be obtained from Techne or your Techne dealer:
Item No
Description
Quantity
FTU05TW
0.5ml microtubes, thin wall
Pack of 1000 with caps
FTU02TW
0.2ml microtubes, thin wall
Pack of 1000 with caps
FMITUBC
0.2ml microtubes, thin wall
125 strips of 8
FMICAPC
0.2ml micro tube caps
125 strips of 8
F96T02
96 well plate, flat top non-skirted, pad of 25 plates
pack of 25
F96PLNS
96-well plate raised top non-skirted
Case of 50
F96PLHS
96-well plate half skirt
Case of 50
F96SEAL
FTC51BHD
FTC51BFD
FTC51B5D
FTC51BID
FTC5B384
96-well sealing mat (silicone)
TC-512 block 96 x 0.2ml microtube or 96 well plate
TC-512 block 96 x 0.2ml microtube or 96 well plate
TC-512 block 40 x 0.5ml microtube
Flat plate block for in situ (non TC-512)
TC-512 block 384 well plate
Case of 50
1
1
1
1
1
For full details of “Gensoft” contact your dealer or Techne Sales Department.
Replacement parts
The following replacement parts can be obtained from Techne or your Techne
dealer:
Item Nº
Description
Quantity required
FCABRTUK
Mains cable and plug UK 230V
1
FCABRTEU
Mains cable and Schuko plug 230V
1
FCABRTUS
Mains cable and US 3-pin plug 120V
1
FCABRTUS
Mains cable and US 3-pin plug 100V
1
6500342
Fuse T6.3A, 230V units
2
6008445
Fuse T8A, 120V units
2
6500324
Fuse T10A, 100V units
2
6104172
Non-Volatile Memory Module
1
PAGE 39
TC-512
OPERATOR’S
MANUAL
Notes
PAGE 40
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