ECLIPSE 90i/80i Accessories
Digital-imaging Heads, Universal Epi-fluorescence Illuminator Specifications
DIH-E
Applications
DIH-M
Universal Epi-fluorescence Illuminator
Epi-fluorescence, Epi-brightfield, Epi-darkfield, Epi-DIC, Epi-simple polarizing, Confocal
Observation/front port/rear port: 100/0/0, 0/100/0, 0/0/100
Light distribution
Motorized switching
Depends on eyepiece tube used
Manual switching
Font port: 1X, diameter ø52mm
Optical output ports
Rear port: optical zoom 0.8-2.0X (continuous), zoom ratio 2.5 : 1, C-mount
Motorized
Inclination angle
25°
F.O.V.
22, 25mm
Filter turret
Not available
Manual
Accessories
Depends on eyepiece tube used
6 filter cubes mountable
Motorized
Manual
Hi S/N Noise Terminator mechanism
Available
Excitation light balancer
Available
Aperture diaphragm
Centerable, detachable, diameter ø1-9mm (manual)
Field diaphragm
Centerable, not detachable, diameter ø1-9mm
Motorized
ND filters
Advanced Research Microscope Accessories
Manual
ND4, ND8, ND16 (manual)
Excitation light shutter
Motorized
Analyzer
Motorized
Manual
Polarizer
Manual (with slot)
Light source
Mercury, xenon, halogen (centerable)
External connection
USB, C1 interlock, external connection connector
Status-check function
Available (data can be recorded with image captured by Digital Sight digital camera)
Not available
Compatible microscopes
90i, 80i
90i, 80i, E600, E600FN, ME600L, L150
Manual (with slot)
Not available
Specifications and equipment are subject to change without any notice or obligation
on the part of the manufacturer. April 2004.
©2004 NIKON CORPORATION
WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS
CAREFULLY BEFORE USING YOUR EQUIPMENT.
ISO 9001
Accredited by the
Dutch Council for
Accreditation
NIKON CORPORATION
Instruments Company
ISO 14001
NIKON CORPORATION
Yokohama Plant
Company names and product names appearing in this brochure are their registered trademarks or trademarks.
NIKON INSTECH CO., LTD.
NIKON INSTRUMENTS EUROPE B.V.
NIKON INSTRUMENTS INC.
Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku,
Kawasaki, Kanagawa 210-0005, Japan
phone: +81-44-223-2167 fax: +81-44-223-2182
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www.nikon-instruments.com/
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phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306
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MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387
ECLIPSE 80i
ECLIPSE 90i
ECLIPSE i-Series Website
NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
Tokyo 100-8331, Japan
www.nikon.com/
Printed in Japan (0404-12)T
Code No. 2CE-MSLH-1
www.nikon-i.com
This brochure is printed on recycled paper made from 40% used material.
En
Epi-fluorescence
Epi-fluorescence
Epi-fluorescence Accessories
The digital-imaging head and the universal epi-fluorescence illuminator incorporate
Nikon’s unique Noise Terminator to achieve fluorescence images with an
unprecedentedly high signal-to-noise (S/N) ratio. The digital-imaging head comes in two
types: the DIH-E features a motorized switching for the field diaphragm, optical path,
epi-fl filter cube, excitation light shutter, analyzer and optical zoom, while the DIH-M has
a motorized control specifically for the excitation light shutter.
•S/N ratio has been dramatically improved by directing stray light out of the optical path.
•Excitation wavelength can be continuously fine-tuned by adjusting the sliding distance of the
optional Excitation Balancer.
•Six filter cubes can be accommodated, and they are easily exchanged.
•Filters or mirror in the filter cubes can be easily replaced to create the desired combination.
•Filter position labels glow in the dark for easy identification.
•Universally functional for darkfieled, brightfield, Nomarski DIC and other applications using
episcopic illumination in addition to epi-fluorescence microscopy.
%
6
2
3
1
7
"
8
9
#
!
$
4
&
5
(
"
■ Specific Accessories for Manual Digital-Imaging Head (DIH-M)
4
1
5
8
6
9
7
1 D-DH Digital-Imaging Head M Main Body
9 D-FB Excitation Balancer (optional)
2 D-CB Connector Box
! D-ES ND Filters
3 C-HS Hand Switch
" C-FC Epi-fl Collector Lens
4 Filter Position Labels
# Light Shielding Tube
5 Light Shielding Plate
$ C-FC Centering Tool
6 Hg Lamphouse HMX-4B
% C-SHG1 Power Supply for HG 100W
7 Aperture Diaphragm
& C-FL Epi-fl Filter Cubes
8 Field Diaphragm
( CFI Plan Fluor Objectives
!
2
#
3
7
$
■ Specific Accessories for Universal
Epi-fluorescence Illuminator
6
■ Specific Accessories for Motorized Digital-Imaging Head (DIH-E)
1 D-FL Universal Epi-fluorescence
Attachment
1 D-DH-E Digital-Imaging Head E Main Body
8 C-FC Epi-fl Collector Lens
2 Filter Position Labels
2 Filter Position Labels
9 Light Shielding Tube
3 Light Shielding Plate
! C-FC Centering Tool
4 Hg Lamphouse HMX-4B
" C-SHG1 Power Supply for HG 100W
5 Aperture Diaphragm
# C-FL Epi-fl Filter Cubes
6 D-FB Excitation Balancer (optional)
$ CFI Plan Fluor Objectives
3 Light Shielding Plate
8
1
4 C-FC Epi-fl Collector Lens
5 C-FC Centering Tool
6 Hg Lamphouse HMX-4B
2
7 C-SHG1 Power Supply for HG 100W
9
7 D-ES ND Filters
3
5
8 CFI Plan Fluor Objectives
9 C-FL Epi-fl Filter Cubes
4
2
3
Filters
UV-2A
•Standard filter block for UV
•For DAPI, cutting off FITC (green) and TRITC (red)
•Soft-coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off green and red
UV-1A
EX 365/10
DM 400
BA 400
•Narrow band pass – only 365nm (i line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
UV-2B
EX 330-380
DM 400
BA 435
•Darker background than UV-2A
V-2A
EX 380-420
DM 430
BA 450
•Standard filter block for V
BV-2A
EX 400-440
DM 455
BA 470
•Standard filter block for BV
BV-1A
EX 435/10
EM 455
BA 470
•Narrow band pass – only 435nm (g line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
B-3A
EX 420-490
DM 505
BA 520
•Wide band pass – recommended for halogen illumination only
B-2A
EX 450-490
DM 505
BA 520
•Standard filter block for B
•For FITC + Counter-stain (TRITC, PI)
B-2E/C
(FITC)
EX 465-495
DM 505
BA 515-555
•Soft coated type for high signal/noise
•For FITC (green), cutting off Rhodamine red
•Band-pass Barrier Filter used to cut off red
B-1A
EX 470-490
DM 505
BA 520
•Narrower excitation range than B-2A
•FITC+Counter-stain (TRITC, PI)
B-1E
EX 470-490
DM 505
BA 520-560
•For FITC (green), cutting off Rhodamine red
•Band-Pass Barrier Filter used to cut off red
G-2A
EX 510-560
DM 575
BA 590
•Standard filter block for G
G-2E/C
(TRITC)
EX 540/25
DM 565
BA 605/55
•For TRITC (Rhodamine)
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 643nm
G-1B
EX 546/10
DM 575
BA 590
•Narrow band pass – only 546nm (e line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
G-2B
EX 510-560
DM 575
BA 610
•610nm barrier provides darker background and deep red emission
Y-2E/C
(Texas Red)
EX 540-580
DM 595
BA 600-660
•For Texas Red®
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 660nm
G
Y
Applications
EX 340-380
DM 400
BA 435-485
BV
B
Characteristics
UV-2E/C
(DAPI)
UV
V
Wavelengths
EX 330-380
DM 400
BA 420
Nomarski DIC
Epi-fluorescence Filters
Nomarski DIC Accessories
Fluorescence Filter Cubes
•DAPI
•Hoechst 33258/33342
•AMCA
•Cascade Blue®
•Autofluorescence
The new DIC system produces well-balanced images with outstanding
contrast and resolution to produce crisp and clear images with perfectly even
brightness, even during low-magnification observations.
• Two types of DIC modules (dry) are enough to cover observations from 10X to 100X.
• DIC sliders can be chosen from Standard, High-contrast, and High-resolution types,
depending on the specimen.
• Shear direction of DIC images can be adjusted* on a rotatable stage.
*Rotatable stage cannot be mounted on the 80i mechanical-stage model.
•Catecholamine
•Serotonin
•Tetracycline
•Quinacrine
•Quinacrine Mustard (QM)
•Thioflavine S
•Acriflavine
■ Specific Accessories for 90i System
1
1 D-CUD-E Motorized Universal Condenser Dry
3
2 D-C DIC Module Dry
3 D-ND6-E Motorized Sextuple DIC Nosepiece
5
4 D-DP DIC Rotatable Polarizer
6
5 D-DA DIC Analyzer
4
•FITC
•Acridine Orange
•Auramine O
•Coriphosphine O
•Bodipy®
•Fluo-3
•DIO
6 D-LP Lambda Plate
7
2
7 D-C DIC Sliders
8 CFI Plan Fluor/CFI Plan Apo Objectives
9 C-CT Centering Telescope
8
9
•TRITC
•Rhodamine B200
•Propidium iodide
•R-Phycoerythrin
•B-Phycoerythrin
•Dil
•Ethidium Bromide
■ Specific Accessories for 80i System
3
4
1
1 D-CUD Universal Condenser Dry
2 D-C DIC Module Dry
3 D-ND6 Sextuple DIC Nosepiece
4 D-NID6 Intelligent Sextuple DIC Nosepiece
6
•Texas Red®
2
5 D-DP DIC Rotatable Polarizer
7
5
6 D-DA DIC Analyzer
8
7 D-LP Lambda Plate
8 D-C DIC Sliders
Fluorescence Reagents and Dedicated Filters
for Fluorescence Protein
Wavelengths
Filters/Reagents
9 CFI Plan Fluor/CFI Plan Apo Objectives
*1
GFP-L
GFP-B
BFP
CFP
YFP
Cy 3
Cy 5
Cy 7
4
EX480/40, DM505, BA510
EX480/40, DM505, BA535/50
EX380/30, DM420, BA460/50
EX436/20, DM455, BA480/40
EX500/20, DM515, BA520
EX535/50, DM565, BA610/75
EX620/60, DM660, BA700/75
EX710/75, DM750, BA810/90
Multi-Band Filters*1
Filters
Abbreviations Reagents
F-R
FITC/Rhodamine
Dual
Triple
F-T
FITC/Texas Red
D-F
DAPI/FITC
D-F-R
DAPI/FITC/Rhodamine
D-F-T
DAPI/FITC/Texas Red
! C-CT Centering Telescope
9
!
Filters are consumables. Filters marked with an asterisk (*1), in particular, come with special coatings,
so these should be replaced in the shortest possible period of time.
Other filter combinations are available. Please ask your local Nikon distributor for a complete list of filters
or inquire about special custom filter combinations possible.
5
This method allows a DIC image to be overlaid with an epifluorescence image within a single view field. This makes the position
identification of a fluorescence specimen easier and more accurate.
In configuration with the DIH-E motorized digital-imaging head and 90i,
the system can automatically optimize settings for epi-fl/DIC
observations at the click of a mouse via the connected PC.
■ Specific Accessories for 90i System
1 D-DH-E Digital-imaging Head E Main Body
2 Filter Positon Labels
3 Light Shielding Plate
4 D-ES ND Filters
5 D-FB Excitation Balancer (optional)
6 Aperture Diaphragm
)
%
7
1
&
9
4
!
8 D-C DIC Module (Dry)
■ Specific Accessories for Rotatable
Mechanical Stage Model
■ Specific Accessories for Mechanical
Stage Model
9 Light Shielding Tube
1 D-CUD Universal Condenser (Dry)
1 C-C Phase Contrast Condenser
2 D-C PH Module
2 CFI Plan Fluor DLL Objectives
3 CFI Plan Fluor DLL Objectives
3 Filter, 45mm GIF
$ D-C DIC Slider
(
$
4
2
1
2
# C-FC Centering Tool
#
1
3
*For phase contrast applications on the 80i rotatable mechanical stage model,
a universal condenser must be used.
" D-DA DIC Analyzer
5
2
These accessories produce well-defined high contrast images with
neutral background coloration regardless of the magnification range.
The CFI Plan Fluor DLL objectives can be universally used for phase
contrast, epi-fluorescence, brightfield, as well as DIC applications.
The CFI Achromat ADL objectives clearly visualize the minute
structure of thick phase objects by minimizing halos.
7 D-CUD-E Motorized Universal Condenser Dry
! D-DP DIC Rotatable Polalyzer
8
6
Phase Contrast Accessories (80i)
Phase Contrast
Epi-fluorescence / DIC
Epi-fluorescence/Nomarski DIC Accessories
4 C-CT Centering Telescope
3
% Hg Lamphouse HMX-4B
"
3
& C-FC Epi-fl Collector Lens
) C-SHG1 Power Supply for HG 100W
~ C-FL Epi-fl Filter Cubes
+ CFI Plan Fluor/CFI Plan Apo Objectives
)
!
4
1
Darkfield
( D-ND6-E Motorized Sextuple DIC Nosepiece
+
~
Darkfield Accessories
These dedicated darkfield accessories allow clear observation of
blood or the minute structure of flagella. Dry- and oil-type
condensers are available. The expander lens is used to obtain
illumination with greater brightness. It is also possible to perform
darkfield microscopy using a universal condenser or phase contrast
condenser.
5
1
2
3
■ Specific Accessories
1 C-CEL Expander Lens
2 C-C Darkfield Condenser (Dry)
3 C-C Darkfield Condenser (Oil)
6
7
"
2
&
.
~
#
9
3
%
Simple Polarizing Accessories
+
$
,
(
" D-C DIC Module (Dry)
-
/
# Light Shielding Tube
Ideal for observing birefringent samples such as collagen, amyloids
and crystals. Operation is as simple as inserting a polarizer over the
field lens and an analyzer in the arm slot. The field number has been
increased to 25.
1
2
■ Specific Accessories
1 C-SP Simple Polarizer
■ Specific Accessories for 80i System
$ D-DP DIC Rotatable Polalyzer
1 D-DH Digital-imaging Head M Main Body
% D-DA DIC Analyzer
2 Filter Positon Labels
& C-FC Centering Tool
3 Light Shielding Plate
( D-C DIC Slider
4 D-CB Connector Box
) Hg Lamphouse HMX-4B
5 C-HS Hand Switch
~ C-FC Epi-fl Collector Lens
6 Aperture Diaphragm
+ D-ND6 Sextuple DIC Nosepiece
7 Field Diaphragm
, D-NID6 Intelligent Sextuple DIC Nosepiece
8 D-FB Excitation Balancer (optional)
- CFI Plan Fluor/CFI Plan Apo Objectives
■ Specific Accessories
9 D-ES ND Filters
. C-SHG1 Power Supply for HG100W
1 C-TP Polarizer with First-order Red Tint Plate
! D-CUD Universal Condenser Dry
/ C-FL Epi-fl Filter Cubes
2 C-IA Intermediate Tube with Analyzer
2 C-ISA Intermediate Tube w/Simple Analyzer
Sensitive Color Polarizing
6
Simple Polarizing
8
Sensitive Color Polarizing Accessories
Enables gout and pseudo-gout tests easily.
1
2
7
Accessories
Accessories
Other Accessories
Eyepiece Tubes
Stages
Ergo Controller (90i only. Available soon)
Magnification module
The image can be rotated and the polarizing direction adjusted on the
rotatable stage. The specimen can be moved in the XY direction on the
motorized stage*.
The Ergo Controller allows focus
adjustments, stage movements and
objective lens changeovers to be performed
in front of a PC monitor as if operating the
actual microscope.
The turret system allows the intermediate
magnification to be changed to 1X, 1.25X,
1.5X or 2X.
*
Name
Beam Split Ratio
Field Number
Binocular Tube B
Observation 100%
22
Trinocular Tube FUW
Observation 100% / Photo 100%
22/25
Trinocular Tube TUW
Observation 100% / Photo 100% /
Observation 20%, Photo 80%
22/25
Ergonomic Binocular Tube
DSC Port (for Ergo Tube)
Observation 100% /
Observation 50%, Photo 50%
(with DSC port)
ISO Photo Tube
Attachable
C-SR Mechanical Stage (for 80i mechanical stage)
FX-III series photomicrographic equipment
C-SRR Rotatable Mechanical Stage (for 90i/80i rotatable mechanical stage)
The FX-III series utilizes a direct-projection system with a swing-out prism
for fast exposure setting and accurate metering.
U-III: 0.1% and 1% spot exposure, and 35% integrated-average
measurement modes
H-III: 1% spot and 35% integrated-average measurement modes
P-III: Manual exposure model
D-S-E Motorized XY stage* (90i only)
Specimen holders available separately.
*Available soon.
Attachable
Motorized ND Filter Unit (90i only. Available soon)
22
With C-mount 0.7X lens
Specimen Holders
The Motorized ND Filter Unit
automatically selects the optimum
filter type after a change of the
objective.
2-slide holders and a single-slide
holder enabling the quick exchange
of the specimen with one hand are
available.
Eyepiece Lenses
Condensers
H-III
Teaching Heads
Name (Magnification)
Field Number
CFI 10X
22
CFI 10XM
22
CFI 12.5X
16
CFI 15X
14.5
CFI UW 10X
25
CFI UW 10XM
25
Various types from 2-person (side by side or face-to-face) to 10-person
types are available.
Reticule
Photomask
Photomask
Nosepieces
The DIC-type nosepiece allows researchers to individually mount DIC
sliders for each objective. The intelligent-type nosepiece allows objective
information to be recorded with the image when it is captured with a Digital
Sight–series digital camera. Two motorized types are available for the 90i,
according to use.
Type
N.A.
W.D.
(mm)
1.9
4.2
3.2
1.6
10.2
10.2
4
1.5
1.9
2.3
1.6
2.3
Objective Mag.
(F.O.V. 22)
Abbe Condenser
0.9
4-100X
Achromat Condenser
0.85
4-100X
Achromat Swing-out Condenser 1-100X
0.8/0.12
1-100X
Achromat Aplanat Condenser
1.40
10-100X
LWD Achromat Condenser
0.65
4-40X
Low Power Condenser
0.15
1-4X
Darkfield Condenser (Dry)
0.8-0.95
10-40X
Darkfield Condenser (Oil)
1.2-1.43
20-100X
Phase Contrast Condenser *1
0.9
10-100X
Universal Condenser (Dry)
0.9/0.13
2-100X
DIC Condenser (Oil)
1.4
10-100X
Motorized Universal Condenser (Dry)*2
0.9/0.13
2-100X
*1 Cannot be mounted on the 90i and 80i rotatable mechanical stage models. *2 90i only.
Quadrocular Adapter
Two CCTV cameras or one CCTV camera and a
digital camera can be attached to the trinocular
eyepiece tube while maintaining the same eye level.
5-person type
Double-lamphouse Adapter
Drawing Tube
The drawing tube allows both the image of a
specimen and the drawing to be seen through
the eyepieces. A dedicated adapter is necessary
to attach it.
The double-lamphouse adapter allows
two different light sources to be
simultaneously attached to the
microscope, eliminating the need to
change the lamphouse and carry out
time-consuming centering procedures.
CCTV Adapters
2.5X, 4X relay lenses are ideal for video enhanced contrast (VEC
applications. Use 0.35X, 0.45X and 0.7X relay lenses for 1/3-, 1/2-, 2/3-inch
CCD cameras, respectively. 0.9 to 2.2X zoom relay lenses are also available.
Double port
8
C-N6
Sextuple Nosepiece (80i)
C-ND6
Sextuple DIC Nosepiece (80i)
D-NID6
Intelligent Sextuple DIC Nosepiece (80i)
D-NI7
Intelligent Septuple Nosepiece (80i)
D-ND6-E
Motorized Sextuple DIC Nosepiece (90i)
D-N7-E
Motorized Septuple Nosepiece (90i)
The double port enables the use of one CCTV camera
and one digital camera, or two digital cameras
simultaneously.
9
Brightfield
(CFI)
4X
0.10
30.00
-
4X
0.13
17.10
-
10X
0.25
7.00
-
10X
0.30
16.00
0.17
LWD 20X
0.40
3.90
0.17
20X
0.50
2.10
0.17
40X
0.65
0.65
0.17
0.35
0.17
0.55
2.7-1.7
0-2.0
20XMi
(Multi-immersion)
0.75
LWD 40XC
60X
0.80
0.30
0.17
40X
0.75
0.72
0.17
1.30
0.20
0.17
0.85
0.30
0.11-0.23
DL 10X
0.23
0.17
0.25
7.00
-
Achromat
0.17
LWD DL 20XF
0.40
3.10
1.2
DL 40X
0.65
0.65
0.17
ADL 10X
New Phase LWD ADL 20XF
Contrast
LWD ADL 40XF
(CFI)
0.55
1.25
0.25
2.7-1.7
0.23
6.10
6.20
1.2
3.10
1.2
0.55
2.10
1.2
0.55
2.7-1.7
0-2.0
P 4X
0.10
30.00
-
P 10X
0.25
7.00
-
LWD P 20X
0.40
3.90
0.17
1.25
0.23
0.17
UW 1X
0.04
3.20
-
0.06
7.50
-
4X
0.10
30.00
-
Brightfield
10X
0.25
10.50
-
(CFI Plan)
20X
0.40
1.20
0.17
Plan Achromat
40X
0.65
0.56
0.17
50XH
0.90
0.35
-
100XH
1.25
0.20
0.17
No Cover
NCG 40X
0.65
0.48
0
(CFI Plan)
NCG 100X
0.90
0.26
0
DL 10X
Phase
Contrast
(CFI Plan)
0.25
10.50
-
1.2
1.2
DLL 10X
0.30
16.00
-
(CFI Plan Fluor) DLL 20X
DLL 40X
0.50
2.10
0.17
0.75
0.72
0.17
DLL 100XH
1.30
0.20
0.17
2X
0.10
8.50
-
4X
0.20
15.70
-
10X
0.45
4.00
0.17
20X
0.75
1.00
0.17
40X
0.95
0.14
0.11-0.23
Brightfield
40XH
1.00
0.16
0.17
(CFI Plan Apo)
60X
0.95
0.15
0.11-0.23
60XH
1.40
0.21
0.17
VC 60XH
1.40
0.13
0.17
VC 60XWI
1.20
0.27
0.15-0.18
0.17
Evanescent
0.17
(CFI Plan Apo)
Fluor
Super Fluor
Epi-fl
20XW
0.50
2.00
0
(CFI Fluor)
40XW
0.80
2.00
0
60XW
1.00
2.00
0
4X
0.20
15.5
-
10X
0.50
1.20
0.17
20X
0.75
1.00
0.17
(CFI S Fluor) 40X
0.90
0.30
0.11-0.23
1.30
0.22
0.17
0.5-1.3
0.20
0.17
40XH
100XH( iris)
VC 100XH
1.40
0.13
NCG 100XH
1.40
0.17
0
TIRF 60XH
1.45
0.13
0.10-0.22
1.45
0.13
0.17
DM 20X
0.75
1.00
0.17
DM 40X
0.95
0.14
0.11-0.23
DM 40XH
1.00
0.16
0.17
DM 60X
0.95
0.15
0.11-0.23
DM 60XH
1.40
0.13
0.17
DM 100XH
1.40
0.13
0.17
TIRF
(CFI Plan Apo) TIRF 100XH
0.17
0
0.17
15.20
0.56
0.17
0.2
0.30
1.20
2.00
1.30
DL 10X
0.65
0.20
100XH
0.17
0.40
0.30
0.14-0.20
0.2
DL 40X
1.25
0.17
0.30
16.40
DL 20X
10XW
0.22
0.90
0.13
Phase
Contrast
DL 100XH
Epi-fl
Plan Apochromat
UW 2X
0.5-1.25
100X
0.5-1.3
0.17
P 100XH
N.A.
DL 4X
Phase
Contrast
-
0.40
0.65
60XSH ( iris )
100XH ( iris )
0.17
0.25
0.65
60X
0-2.0
LWD ADL 40XC
P 40X
10
0.5-1.25
3.90
LWD DL 40XC
Epi-fl
Name
(CFI Plan Fluor) 40XH
0.17
0.40
BM 10XA
(CFI)
0.23
LWD DL 20X
DL 100XH
Polarizing
1.25
Use
Plan Fluor
100XH( iris)
(CFI)
Cover-glass
Thickness
W.D.
(mm)
100XH
Phase
Contrast
W.D.
(mm)
N.A.
Name
Cover-glass
Thickness
Type
Type
Use
90i/80i Specifications
CFI Objectives
Eclipse 90i/80i Specifications
CFI Objectives
90i
Main body
Optical system
80i
CFI60 infinity optical system
Base
Rotatable stage model
Mechanical stage model, Rotatable stage model
Transmitted
illumination
12V-100W halogen lamp
External transformer
Fly-eye lens built-in
NCB11, ND8, ND32 filters built-in (detachable)
Diffuser built-in (not detachable)
Light-intensity control via motorized ND filter unit (option)(available soon)
12V-100W halogen lamp
Transformer built-in
Fly-eye lens built-in
NCB11, ND8, ND32 filters built-in (detachable)
Diffuser built-in (not detachable)
Controls
Main power switch (power supply)
Light-intensity control
With preset function
ND8/ND32/NCB11 filter insert/remove switch
Motorized control switches (for field diaphragm, aperture diaphragm, stage’s
up/down movement, coarse-fine/ultra-fine focus switching, escape, objective
switching, DIH-E optical-path switching, DIH-E epi-fl filter switching, DIHE/M excitation light shutter)
Ergo Controller for remote control (option) (available soon)
Light-intensity control
With preset function
ND8/ND32/NCB11 filter insert/remove switch
Field diaphragm adjustment ring
Focusing unit
Motorized coaxial coarse/fine focusing
(resolution 0.05µm, linear encoder built-in)
Focusing stroke: 27mm
Refocusing mechanism using escape function
Auto link focus
Manual coaxial coarse/fine focusing (right: fine; left: coarse/fine)
Focusing stroke: 27mm
Coarse: 14mm/rotation; Fine: 0.1mm/rotation
Minimum reading: 1µm
Refocusing mechanism with focus clamp
Eyepiece tubes
Binocular tube B: F.O.V. 22
Trinocular eyepiece tube FUW: F.O.V. 22, 25 (observation/photo: 100/0, 0/100), with reticle rotation correction mechanism
Trinocular eyepiece tube TUW: F.O.V. 22, 25 (observation/photo: 100/0, 20/80, 0/100), with reticle rotation correction mechanism
Ergonomic binocular tube: F.O.V. 22, Inclination angle 10-30°, Tube extension 40mm (DSC port, observation/photo: 100/0, 50/50)
Digital-imaging heads DIH-E/M: F.O.V. 22, 25 (observation/front port/rear port: 100/0/0, 0/100/0, 0/0/100)
Nosepiece
Motorized sextuple DIC nosepiece, Motorized septuple nosepiece
Sextuple nosepiece, Sextuple DIC nosepiece, Intelligent sextuple DIC nosepiece,
Intelligent septuple nosepiece
Stage
Rotatable mechanical stage (centerable; rotation angle 220°)
Cross travel: 54 (Y) x 78 (X)mm, with calibrations;
stage handle height and torque adjustable
Motorized XY stage (option) (available soon)
Specimen holders (2- and 1-slide types)
Rotatable mechanical stage (centerable; rotation angle 220°)
Cross travel: 54 (Y) x 78 (X)mm, with calibrations; stage handle height and torque adjustable
Mechanical stage (centerable; rotation angle 220°)
Cross travel: 54 (Y) x 78 (X)mm, with calibrations; stage handle height and torque adjustable
Specimen holders (2- and 1-slide types)
Condenser
Motorized universal condenser Dry (7 modules mountable), Motorized aperture
diaphragm; Manual condenser can be used via condenser holder
Abbe, Achromat, Achromat Swing-out 1-100X, Low Power, Darkfield (dry, oil),
Achromat Aplanat, Phase Contrast, LWD Achromat, DIC (oil), Universal (dry) condensers
Eyepiece lens
10X (F.O.V.: 22mm), 10X M photo mask (F.O.V.: 25mm), 12.5X (F.O.V.: 16mm), 15X (F.O.V.: 14.5mm), UW 10X (F.O.V.: 25mm), UW 10X M photo mask (F.O.V.: 25mm)
Power consumption
2.8A/230W
2.4A/140W
Weight
18.2kg (standard trinocular set)
13.9kg (standard binocular set)
11
ECLIPSE 90i/80i Accessories
Digital-imaging Heads, Universal Epi-fluorescence Illuminator Specifications
DIH-E
Applications
DIH-M
Universal Epi-fluorescence Illuminator
Epi-fluorescence, Epi-brightfield, Epi-darkfield, Epi-DIC, Epi-simple polarizing, Confocal
Observation/front port/rear port: 100/0/0, 0/100/0, 0/0/100
Light distribution
Motorized switching
Depends on eyepiece tube used
Manual switching
Font port: 1X, diameter ø52mm
Optical output ports
Rear port: optical zoom 0.8-2.0X (continuous), zoom ratio 2.5 : 1, C-mount
Motorized
Inclination angle
25°
F.O.V.
22, 25mm
Filter turret
Not available
Manual
Accessories
Depends on eyepiece tube used
6 filter cubes mountable
Motorized
Manual
Hi S/N Noise Terminator mechanism
Available
Excitation light balancer
Available
Aperture diaphragm
Centerable, detachable, diameter ø1-9mm (manual)
Field diaphragm
Centerable, not detachable, diameter ø1-9mm
Motorized
ND filters
Advanced Research Microscope Accessories
Manual
ND4, ND8, ND16 (manual)
Excitation light shutter
Motorized
Analyzer
Motorized
Manual
Polarizer
Manual (with slot)
Light source
Mercury, xenon, halogen (centerable)
External connection
USB, C1 interlock, external connection connector
Status-check function
Available (data can be recorded with image captured by Digital Sight digital camera)
Not available
Compatible microscopes
90i, 80i
90i, 80i, E600, E600FN, ME600L, L150
Manual (with slot)
Not available
Specifications and equipment are subject to change without any notice or obligation
on the part of the manufacturer. April 2004.
©2004 NIKON CORPORATION
WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS
CAREFULLY BEFORE USING YOUR EQUIPMENT.
ISO 9001
Accredited by the
Dutch Council for
Accreditation
NIKON CORPORATION
Instruments Company
ISO 14001
NIKON CORPORATION
Yokohama Plant
Company names and product names appearing in this brochure are their registered trademarks or trademarks.
NIKON INSTECH CO., LTD.
NIKON INSTRUMENTS EUROPE B.V.
NIKON INSTRUMENTS INC.
Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku,
Kawasaki, Kanagawa 210-0005, Japan
phone: +81-44-223-2167 fax: +81-44-223-2182
www.nikon-instruments.jp/eng/
P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands
phone: +31-20-44-96-222 fax: +31-20-44-96-298
www.nikon-instruments.com/
1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306
www.nikonusa.com/
NIKON FRANCE S.A.
FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33
NIKON GMBH
GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322
NIKON INSTRUMENTS S.p.A.
ITALY phone: + 39-55-3009601 fax: + 39-55-300993
NIKON AG
SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861
NIKON UK LTD.
UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584
NIKON CANADA INC.
CANADA phone: +1-905-625-9910 fax: +1-905-625-0103
NIKON INSTRUMENTS (SHANGHAI) CO., LTD.
CHINA phone: +86-21-5058-5055 fax: +86-21-5058-5060
NIKON SINGAPORE PTE LTD
SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668
NIKON MALAYSIA SDN. BHD.
MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387
ECLIPSE 80i
ECLIPSE 90i
ECLIPSE i-Series Website
NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
Tokyo 100-8331, Japan
www.nikon.com/
Printed in Japan (0404-12)T
Code No. 2CE-MSLH-1
www.nikon-i.com
This brochure is printed on recycled paper made from 40% used material.
En
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