amplifiers - Harvard Apparatus

amplifiers
Products
EC1 73-0822 K Series Force
Transducer, see page 5
Introduction to Physiology
2-3
Transducers
4 - 11
Stimulators
12 - 30
PLUGSYS System Introduction
31 - 32
PLUGSYS System Cases and Minicases
PLUGSYS Modules
EC1 50-6002 6002
Basic Stimulator see page 29
Page No.
33
34 - 62
ISO 510 Amplifier for Bridge-system Transducers
63
1 & 4 Channel Interface Adapter
64
Amplifier Interfaces
65 - 67
Recorders
68 - 73
Electrodes
74 - 80
Data Acquisition Systems
PLUGSYS
HSE Data Acquisition Summary
EC1 40-2000 Harvard
Apparatus TR2 Chart
Recorder, see page 68
81 - 101
82
BDAS W for Data Acquisition
83 - 89
PULMODYN W for Respiratory Studies
90 - 91
ISOHEART W for Isolated Heart Studies
92 - 93
HAEMODYN W for Haeodynamic Studies
94 - 95
ACAD W for Isolated Tissue Studies
96 - 97
NEUROYDN for Neurolgic Studies
98 - 99
PULMODYN Pennock W for Windows
100
HAI 304Ti Data Acquisition Systems
101
HAI 118 Data Acquisition Systems
Protowin for Isolated Tissue Experiments
ADI Powerlab
Chart Pro for Windows
Chart Modules
102 - 103
104
105 - 108
109
110 - 128
ADI Amplifiers
129 - 161
ECG/Biopotential Telemetry System
162 - 165
Sympathetic Nerve/Temp Telemtry System
166 - 171
Kymographs
172 - 179
EC1 50-8259 Complete
Electrophysiological
Teaching Unit, see page 72
1
product groups
NEW 3 TIER APPROACH TO RESEARCH
We are proud to unveil a new structure for our
Physiology, Pharmacology, and Toxicology research
solutions. We’ve organized our products into 3
categories to make your selection of the best
research solution easier.
In the following pages we have complete systems, as
well as individual components, for virtually all facets
of Physiology, Pharmacology and Toxicology
experimentation. By this, we mean several things:
1) Solutions across a range of scientific fields
2) Solutions for a range of technical
applications
3) Solutions designed to achieve different
types of research goals
Harvard Apparatus has always specialized
in providing innovative and reliable
research solutions.
This final point makes Harvard Apparatus unique. From
our own experience in the lab, we realize that the same
experiment may not have the same goal in all labs.
For example, a contraction force experiment in skeletal
muscle requires a tissue bath. But the goal of the study
(metabolic quantification, compound screening,
teaching) will dictate the right tissue bath.
Our New 3 Tier System is Organized
in the Following Manner:
advanced
research
Harvard Apparatus
HSE Advanced
Research
This tier offers cutting edge
research systems with
advanced features for
ultimate physiological
stability and experimental flexibility. Guided by decades of in-the-field
research and development, Harvard Apparatus HSE Advanced Research
solutions are the engines of breakthrough research.
With more capability packages and measurement possibilities than any
comparable system, Harvard Apparatus HSE Advanced Research systems
allow physiological recording to be more integrated than ever before. In
addition, modular engineering and construction allows options and
capabilities to be added at any time as the goals and demands of your
research change.
Harvard Apparatus
Research
research
This tier is designed for
functionality and ease of use,
Harvard Apparatus Research
systems are precision systems
for the routine or standard
measurements in any lab. Harvard Apparatus Research systems are also
perfect for compound screening or rapid-throughput experimentation.
Harvard Apparatus
Teaching
teaching
This third tier of equipment
was designed for the needs
of teaching labs. The products
are constructed to meet a
special set of demands, especially where usage is high and regular
maintenance is a challenge. Harvard Apparatus Teaching systems are
exceptionally rugged, reliable and accurate, while representing an
excellent value.
Please contact our technical experts at
physiology@harvardapparatus.com
For a custom configuration and full system quotation.
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
2
product groups
NEW 3 TIER APPROACH TO RESEARCH (continued)
PLUGSYS Modular Measuring and Control System
Research Isometric Transducer
Product Group
advanced
research
research
Harvard Apparatus HSE
Advanced Research
Harvard Apparatus
Research
teaching
Harvard Apparatus
Teaching
Page #
Transducers
F30 and F40 Isometric Transducers
X
4
K Series Force Transducer
X
5
Vernier Control Units
X
5
TRI Series Isometric Force Transducers
X
Research Isometric Force Transducers
X
UF1 Series Isometric Force Transducer
7
X
Isometric Transducer
B40 Isotonic Transducer
6
X
X
TRO Isotonic Transducer
8
8
10
X
11
Battery Powered Isotonic Transducer
X
10
Teaching Isotonic Transducer
X
11
Stimulators
HA-HSE Stimulator 1
X
15 - I21
Universal Isolated Stimulator Output
X
22
HA-HSE Stimulator C
X
23
HA-HSE Stimulator CS
X
24
D330 MultiStim System
X
25 - 27
LE Series Stimulators
X
28
6000 Series Stimulors
X
29 - 30
Amplifiers
PLUGSYS System
X
ISO 510 Transducer Amplifier
31 - 62
X
63
Complete Electrophysiology Teaching Unit
X
72 - 73
1 - 4 Channel Interface Adapter & Modules
X
64 - 67
ADI Amplfiers
Recorders
X
TR2 Chart Recorder
X
68
Modular Universal Oscillographs
X
69 - 70
129 - 161
Student Oscillograph
X
71
Universal Kymographs
X
172 - 179
Data Acquistion
HA-HSE Data Acquistion Systems
X
81 - 99
iWorx Data Aquisition Systems
X
101 - 103
Protowin Software
X
104
ADI Data Acquisition Systems
X
105 - 128
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
3
advanced
research
transducers
F Series Isometric Force Transducers
• Resolution of ±2 mg
• Ultra low vertical
displacement (1 µm/g)
• Suitable for small
tissue samples like
papillary muscle,
Purkinje fibers and
vessel rings
• Force Range of ±30
grams (±0.3 N)
HA-HSE Isometric Force Transducer F30
The HA-HSE F30 is a sensitive, reliable and rugged isometric force transducer
suitable for use with very small muscle/tissue specimens. The ultra-low vertical
displacement of the transducer means that true isometric measurements can
be made. The low seismic sensitivity of the HA-HSE F30 minimizes the
influence of environmental vibrations on the force recordings which result in
very stable baselines. The design of the unit (no internal wires suspending the
transducer head) means that this transducer will stand up to years of use. This
is a full resistance bridge transducer which can be connected to the HA-HSE
TAM-A and TAM-D strain gauge amplifiers, see page 36. Connectors are also
available for Harvard Apparatus Ltd., Grass and Gould strain gauge amplifiers.
Specifications
Force Range
Linear Range
Maximum Load
Displacement Range
Sensitivity
Isometric Quotient IQ
Excitation AC or DC
Full-Scale Output
Natural Frequency
Bridge Resistance
Hysteresis
Zero Drift
Resolution (Noise)
Maximum Load
Breaking Load
Sensitivity to
Transverse Load
Maximum Allowed
Transverse Load
Connection Cable
Weight
Dimensions
Mounting Rod, OD x L
Order #
• Ultra-high resolution
of ±0.5 mg
• Low vertical
displacement (6 µm/g)
• Suitable for small
tissue samples like
papillary muscle,
Purkinje fibers and
vessel rings
• Force range of ±10
grams (±0.1 N)
HA-HSE Isometric Force Transducer F10
The HA-HSE F10 isometric force transducer is a lower force range, higher
resolution version of the F30 isometric force transducer on the left.
* Note: This transducer is produced under licence from DLR: Deutsche Forschungsanstalt für
Luft- und Raumfahrt e.V., D-51140 Köln.
Specifications
Force Range
±10 g (±0.1 N)
Linear Range
±20 cN
Maximum Load
±0.5 N
Displacement Range
±60 µm
Sensitivity
3.0 mV/V g
±30 g (±0.3 N)
±50 cN
±3 N
±0.03 mm
0.5 mV/V g
1 µm/g
5 V (max. 10 V)
±10 mV/V, nominal
440 Hz, nominal
1 KΩ, nominal, full resistance bridge
< 0.1%
< 10 mg/°C at 20 to 40°C < 0.03 % FS/°C
2 mg
300%
1000%
< 0.1%
Isometric Quotient IQ
6 µm/g
Excitation AC or DC
5 V (max. 10 V)
< 300%
Order #
1.5 m L
200 g (7 oz)
22 x 22 x 70 mm (0.9 x 0.9 x 2.8 in)
6 x 130 mm (0.25 x 5.1 in)
EC1 73-0841 Isometric Transducer F10 for PLUGSYS Amplifier
Product
EC1 73-0831 Isometric Transducer F30 for PLUGSYS Amplifier
EC1 73-2657 Isometric Transducer F30 for Harvard Apparatus
Transducer Amplifier
EC1 73-2658 Isometric Transducer F30 for Grass Amplifier
EC1 73-2659 Isometric Transducer F30 for Gould Series 6600
Amplifier
Full-Scale Output
±20 mV/V, nominal
Natural Frequency
180 Hz, nominal
Hysteresis
1 KΩ, nominal, full Ωic bridge
Zero Drift
< 10 mg/°C at 20 to 40°C < 0.03 % FS/°C
Resolution (Noise)
0.5 mg
Bridge Resistance
< 0.1%
Maximum Load
100%
Breaking Load
300%
Connection Cable
1.5 m L
Peak to Peak
< 0.5 mg
Weight
200 g (7 oz)
Dimensions
22 x 22 x 70 mm (0.9 x 0.9 x 2.8 in)
Mounting Rod, OD x L
6 x 130 mm (0.25 x 5.1 in)
Product
EC1 73-2661 Isometric Transducer F10 for Harvard Apparatus
Transducer Amplifier
EC1 73-2662 Isometric Transducer F10 for Grass Amplifier,
6-Pin Cannon Connector
EC1 73-2663 Isometric Transducer F10 for Gould Series 6600
Amplifier
References:
Gollasch, M. et al.: Pinacidil Relaxes Porcine and Human Coronary Arteries by Activating ATPDependent Potassium Channels in Smooth Muscle Cells. J. Pharmacol. Exp. Ther., 275. 681691 (1995)
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
4
transducers
K Series Force Displacement
Transducers & Vernier Control
advanced
research
EC1 73-0822
850 N
•
•
•
•
850 S
850 E
Four ranges available
Oil damping system
Robust construction
Application in teaching
K Series Force-Displacement Transducers
Series K force transducers are universally applicable for the measurement
of force. There are 4 models, for 4 different force ranges: K30 (±30 gforce), K100 (100 g-force), K300 (300 g-forces), and K1000 (1000 g-force).
The maximum displacement at the tip of the flat spring is 4 mm. The K30
includes a damping system with oil.
• 3 versions available
• Robust mechanical construction with less friction
• Allows fine adjustment
Specifications
The vernier control allows an exact pre-stretch of the tissue preparation in
order to ensure optimum contractile force of the musculature. The SVersion is specific for the transducer with 14 mm mounting rod. The EVersion is a motorized type that is compatible with the flushing unit and
microdrive controller.
Transducer
K30
K100
K300
K1000
Force Range
±30 g-force,
±0.3 N
±2.5 mm
100 µV/
g-force/V
1200 µV/
mm/V
110 Hz
±100 g-force,
±1N
±2 mm
30 µV/
g-force/V
0.7 mV/
mm/V
170 Hz
±300 g-force,
±3 N
±3 mm
10 µV/
g-force/V
0.8 mV/
mm/V
200 Hz
±1000 g-force,
±10 N
±1 mm
3 µV/
g-force/V
1.2 mV/
mm/V
400 Hz
Displacement
Sensitivity
(Force)
Sensitivity
(Displacement)
Natural Frequency
without Load
Resolution
Input Impedance
(Pins 1–5)
Output Impedance
(Pins 2–4)
Supply Voltage
Dimensions
Without Pulley
Height with Pulley
Mounting Rod
Weight
5 mg
350 Ω
15 mg
50 mg
150 mg
350 Ω
10 V max.
20 x 20 x 84 mm (0.8 x 0.8 x 3.3 in)
Vernier Control
Specifications
Weight:
850 N and 850 S
0.35 kg (0.8 lb)
850 E
0.47 kg (1 lb)
Max. Dimensions:
850 N and 850 S
115 x 50 x 20 mm (4.5 x 2 x 0.8 in)
850 E
160 x 55 x 20 mm (6.3 x 2.2 x 0.8 in)
Displacement Per Turn
0.5 mm
Max. Displacement
22 mm
Rod Mounting Hole Ø:
60 mm (2.4 in)
6 x 130 mm (0.25 x 5.1 in), OD x L
200 g (7 oz)
850 N and 850 E
13 mm (0.5 in)
850 S
14 mm (0.55 in)*
Hole for Transducer Holder:
K Series Force Transducer
850 N and 850 E
2 x Ø 10.5 mm/90°
850 S
2 x Ø 14.5 mm/90°*
Power Supply 850 E Only 12 V DC for connection to
flushing unit
Transducer Model
For Use
With
K30
K100
K300
K1000
HA-HSE
EC1 73-0822
EC1 73-0824
EC1 73-0825
EC1 73-0826
Order #
HA
EC1 73-2676
EC1 73-2677
EC1 73-2678
EC1 73-2679
EC1 73-0786 Vernier Control, Standard, Type 850 N
Grass
EC1 73-2680
EC1 73-2681
–
EC1 73-2683
Gould 6600 EC1 73-2684
EC1 73-2685
EC1 73-2686
EC1 73-2687
*Note: Extra large hole for GRASS force-displacement transducers
Product
EC1 73-0787 Vernier Control, Motorized Version, Type 850 E
EC1 73-0788 Vernier Control, Special, Type 850 S
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
5
research
transducers
TRI Series Isometric Force Transducers
TR 201 Isometric Force Transducer
TRI Series Force Transducers (isometric) measure the force of contraction
of isolated tissues under conditions of constant force. Isometric
Transducers are classically used in Organ Bath experiments with muscles
with short/rapid contractions such as tracheal muscle, atrium, papillary
muscle, aortic rings, veins or other vessel rings.
The Transducers are equipped with a special system allowing an easy and
rapid fixation of the thread that tightens upper side of the tissue in an
organ bath. TRI Series Transducers are appropriate for various load ranges
from simple applications to those requiring detection of very small forces.
Specifications
TRI201:
TR 210 Basic Isometric Force Transducer
High performance 5 mg to 25 g; Transducer Range
Resolution
2 mg; Displacement: +- 0.45 mm;
Sensitivity (full range)
30 mv/V; Excitations: 5-10V AC/DC;
Element Resistance
full bridge 360 W
TRI202P-TRI 203P:
0 mg to 25 g (TRI 203P: up to 50 g);
Resolution
infinite;
Displacement
+- 0.03 mm;
Sensitivity
(full range): 100 mv/V;
Excitations
5-10V AC/DC;
Element Resistance
TRI210:
TR 202 Isometric Force Transducer for Small Forces
full bridge 720 W
Teaching Force Transducer
Range
10 mg to 25 g;
Resolution
5 mg;
Displacement
+- 0.5 mm;
Sensitivity
(full range): 20 mv/V;
Excitations
5-10V AC/DC;
Element Resistance
full bridge 360 W
Order #
Parameters Measured
• Muscle contraction force
Components Included
• Transducer
• Specifications Sheet
• Calibration Certificate
Additional components required
• ISO 510 Amplifier in Power Supply Box,
see page 63
Very high sensitivity Transducer for small forces
Range
Model
Product
EC1 76-0094 TRI201
High Performance Transducer
EC1 76-0095 TRI202P
High Sensitivity Transducer from
0 mg to 25 g. (0.05 mg steps)
EC1 76-0096 TRI203P
High Sensitivity Transducer from
0 mg to 50 g. (0.05 mg steps)
EC1 76-0097 TRI210
Basic Force Transducer
EC1 76-0098 TRI210A
Basic Transducer
(audio connector)
Citations
Gomez-Cabrero A et al. (2005) Use of transduction proteins to target trabecular
meshwork cells: outflow modulation by profilin I. Molecular Vision. 11: 1071-1082.
(bovine anterior segment, Spain).
Kim EJ et al. (2005) Safety pharmacology of sibutramine mesylate, an anti-obesity drug.
Hum. Exp. Toxicol. 24(3): 109-119 (rat smooth muscle, Korea)
Rosa EF et al. (2005) Habitual exercise program protects murine intestinal, skeletal, and cardiac
muscles against aging. J. Appl. Physiol. 99(4): 1569-1575. (mice cardiac muscle, Brazil).
Fukada SY et al. (2004) Mechanisms of impaired vascular response to ANGII in
perivascular injured carotid arteries of ovariectomized rat. J. Cardiovasc. Phamacol.
44(3): 393-400. (rabbit carotid arteries, Brazil)
Gomez-Alvis A et al. (2004) Cardiac and vascular effects of diltiazem, dobutamine and
amrinone, drugs used after myocardial revascularization. Braz J Med Biol Res. 37(6):893900. (vessel rings, atrium, Argentina)
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
6
research
transducers
Research Isometric Force Transducers
Our Research Isometric Force Transducers supply
excitation voltage to a self-contained transducer and
convert the pico-farad changes of capacitance into
high level DC voltages using a patented electronic
circuit (U.S. Patent #4,142,144). This circuit is
extremely sensitive and stable, producing up to 5
VDC for direct connection to Standard Signal
Conditioners, Data Acquisition Systems, Modular
Universal Oscillographs, Student Oscillographs,
Flatbed Strip Chart Servo Recorders, and X-Y Plotters.
All transducers have a small external transducer
element hard-wired to a small amplifier. This
EC1 72-4480 to EC1 72-4485 Research Isometric Transducer
amplifier is hard-wired to a wall type transformer. The
transformer reduces line voltage to 12 VDC and is
both UL- and CSA-listed. For 230 VAC, 50 Hz
operation the transformer is VDE-listed. These
Transducers are factory-calibrated to deliver 5 VDC
for full rated load and are still linear for 100%
overload (4 VDC). They have offset (tare) controls.
The Transducer amplifier has a 3-digit backlit LCD
display that shows the output voltage. A standard BNC
output connector is located at the rear of the amplifier.
These amplifiers can be stacked for multiple use.
Specifications
Output Impedance
Sensitivity
10,000 Ω
< 1 part in 1,000
Accuracy Output Voltage ±1%
Output Connector
Standard BNC
Linearity
±1%
Drift
Negligible after 5 min. warm-up
Amplifier Adjustments
Range selector and offset
Display
LCD, 7.6 mm (0.3 in) numbers
Weight
908 g (2 lb)
Transducer, H x W x D
32 x 45 x 32 mm (1-1/4 x 1-3/4 x 1-1/4 in)
Amplifier, H x W x D
51 x 95 x 86 mm (2 x 3-3/4 x 3-3/8 in)
Mounting Handle, OD x L:
• Self-powered transducer and amplifier in one
• Transducers are available with three different
sensitivities
• Each Transducer has two selectable ranges
Research Isometric Transducer
This Isometric Transducer approaches the ideal of measuring isometric
contraction force without motion. It is available with three different force
ranges. Each Transducer has an X10 range selector switch providing
two ranges.
Model 1
12.7 x 51 mm (0.5 x 2 in)
Model 2
9.5 x 91.4 mm (0.375 x 3.6 in)
Research Isometric Force Transducer
Power
115 VAC,
60 Hz
Sensitivity
230 VAC,
50 Hz
Deflection Selectable Offset
2 VDC
Per Gram Force
Control
Output
Load
Ranges
Max. Tare Voltage Per
Model 1 with 12.7 x 51 mm (0.5 x 2 in) Mounting Rod (OD x L)
EC1 72-4490
EC1 72-4493 10.0 µm
0 to 5.0 g
0 to 0.5 g
2.5 g
0.2 g
5.0 g
0.5 g
EC1 72-4491
EC1 72-4494 1.0 µm
0 to 50 g
0 to 5.0 g
25 g
2.5 g
50 g
5g
This Transducer measures force by measuring the change in capacitance
of a stiff beam between two plates. Beam deflection is measured in
microns.
EC1 72-4492
EC1 72-4495 0.1 µm
0 to 500 g
0 to 50 g
250 g
25 g
500 g
50 g
Two models are now available. The only difference between the models is
the length and diameter of the mounting rod. Model 1 has a 12.7mm OD
by 51mm long mounting rod. Model 2 has a 9.5 mm OD by 91.4mm long
mounting rod.
EC1 72-4480
EC1 72-4483 10.0 µm
0 to 5.0 g
0 to 0.5 g
2.5 g
0.2 g
5.0 g
0.5 g
EC1 72-4481
EC1 72-4484 1.0 µm
0 to 50 g
0 to 5.0 g
25 g
2.5 g
50 g
5g
EC1 72-4482
EC1 72-4485 0.1 µm
0 to 500 g
0 to 50 g
250 g
25 g
500 g
50 g
Model 2 with 9.5 x 91.4 mm (0.375 x 3.6 in) Mounting Rod (OD x L)
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
7
transducers
Isometric Transducers
teaching
• ±1% accuracy
• Temperature range from -40° to +80°C
(-104° to +176°F)
• Available in six force ranges
UF1 Series Isometric Transducer
The popular UF1 Series of load cells has been adapted to be used with the
above listed amplifiers and interfaces. These Transducers have an integral
cable that is compatible with the following amplifiers: EC1 53-6132, EC1 507970, EC1 50-7996 and EC1 50-8861. They are available in six force ranges.
Specifications
Dimensions:
Transducer, H x W x D
70 x 35 x 25 mm (2.75 x 1-3/8 x 1 in)
Mounting Rod, OD x L
6.5 x 40 mm (0.25 x 1.5 in)
UF1 Series Isometric Transducers
Order #
• Strain gauge type
• Available in five force ranges
Harvard Apparatus Ltd. Isometric
Transducers
Isometric muscle contraction is defined as force with zero motion. Harvard
Apparatus Isometric Transducers achieve this condition by means of thinfilmed,
bonded strain gauges and substantial beams. The strain gauge has four-wire
closed Wheatstone bridges for long term stability and sensitivity. The stiffness
of the transducer and stem prevents unwanted displacement.
To operate, these Isometric Transducers require power from the amplifier/signal
conditioner to which they are connected. They are available in five force ranges
and are supplied with an amplifier/signal conditioner specific, 1.8 m (6 ft) Cable.
These Cables are also available separately allowing the Transducer to connected
to a different amplifier/signal conditioner simply by switching the Cable.
Specifications
Dimensions:
Force Range
Transducer, H x W x D
19 x 150 x 19 mm (3/4 x 6 x 3/4 in)
EC1 50-7293
0 to 25 g
Mounting Rod, OD x L
12.5 x 75 mm (1/2 x 3 in)
EC1 50-8036
0 to 55 g
EC1 50-8044
0 to 110 g
Harvard Apparatus Isometric Transducer for Use With
EC1 50-8051
0 to 220 g
EC1 50-8069
0 to 450 g
Force
Range
Harvard Apparatus
Amplifiers
Gould Signal
Conditioners
EC1 50-8077
0 to 1000 g
0 to 50 g
0 to 100 g
0 to 200 g
0 to 500 g
0 to 1000 g
EC1 50-7905
EC1 50-7913
EC1 50-7921
EC1 50-7939
EC1 50-7947
EC1 50-7911
EC1 50-7912
EC1 50-7914
EC1 50-7915
EC1 50-7916
Replacement Cable
–
EC1 50-7988
EC1 50-7990
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
8
transducers
Calibration Weights
Force Calibration Weights
Gram Calibration Weights
These NEWTON style calibration weights are precision weights use to
calibrate force transducers. The weights are made from polished brass
and have an integral hook. They are supplied in a wooden storage case.
They are calibrated in Newtons at the standard acceleration of gravity
gn = 9.80665 m/s2. Nine weights are supplied. Class of accuracy M1.
These gram calibration weights are precision weights use to calibrate
preload on force transducers. The weights are made from polished brass.
They are supplied in a wooden storage case with a pair of handling
forceps and a cleaning brush. Nine weights are supplied. The 0.1, 0.2 and
0.5 gram size weights are flat metal pieces. The larger size weights have
an integral hook. Class of accuracy M1 after OIML*.
Specifications
Newtons
Gram-Force
Specifications
0.01 N
1
Weight
0.02 N (2 supplied)
2
0.1 g
0.5 mg
0.05 N
5
0.2 g (2 supplied)
0.6 mg
0.1 N (2 supplied)
10
0.5 g
0.8 mg
0.2 N
20
1g
1.0 mg
0.5 N
50
2 g (2 supplied)
1.2 mg
1.0 N
100
5g
1.5 mg
10 g
2.0 mg
Order #
Product
EC1 73-2634 Force Calibration Weights
Tolerance ± mg
Price $
438.00
Order #
Product
EC1 73-2635 Gram Calibration Weights
Price $
246.00
*Note: Organisation Internationale Metrologie Légale
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
9
Isotonic Transducers
transducers
If the organ preparation is attached to hole no. 10, this is equivalent to a
movement of ±20 mm (= 40 mm total), reducing for each lower hole used. The
unit can be interfaced with almost any type of AC or DC bridge.
There are 3 calibration steps on the frame: ±22 mm, 0 and –22 mm at a
distance of 100 mm from the pivot measured at the arch. The calibration
amplitude is reduced at the various holes as shown below.
Specifications
EC1 73-0857, 73-2692 to
2695 HA-HSE Isotonic Lever
Transducer Model B40
Range/Linearity
±20 mm (0 to 40 mm) / ±2% full scale
Zero Drift
< 0.03% / ˚C FS
Frequency Range
0 to 10 Hz
Full Bridge
• Very low friction, ideal isotonic measurement
conditions
• Easy calibrating conditions
• Large linear measuring range 40 mm
• Ideal for smooth muscle preparations
advanced
research
B40 Advanced Research
Isotonic Lever Transducer
This system is designed to transduce
displacements during contractions and movements into proportional electric
currents. The amount of force required to operate the system is exceedingly
small, providing ideal isotonic measurement conditions for physiological and
pharmacological research. This arrangement of a lever transducer with any
bridge system (DC measuring bridge or TAM plug-in module of our PLUGSYS,
see page 36) is particularly well suited to isotonic measurements in smooth
muscle preparations in isolated organ and tissue baths. Values are
transmitted as a result of movements of a permanent magnet within field
plates.
The instrument is calibrated by locking the end of the lever into the calibration
stops on the frame. These stops represent full deflections of ±10 and ±20 mm.
4 x 300 Ω
Frictional Torque of Lever 0.1 mN cm
±10 mV, adjustable up to max. ±40 mV, internal
resistance of 300 Ω, max. output voltage represents
full lever deflection and full measuring range
Output Voltage
Power Supply
5 V DC or AC (max. 8 V)
Lever
180 x 85 x 40 mm, 3 positions to screw in support rod
Support Rod
6 mm OD x 130 mm L
Hole No.
10
9
8
7
6
5
4
3
2
cm from Pivot
10
9
8
7
6
5
4
3
2
Displacement (mm)
±20
±18
±16
±14
±12
±10
±8
±6
±4
Order #
Product
EC1 73-0857 HA-HSE Isotonic Lever Transducer
Model B40 for PLUGSYS System
EC1 73-2692 HA-HSE Isotonic Lever Transducer
Model B40 for Harvard Apparatus Amplifier
EC1 73-2693 HA-HSE Isotonic Lever Transducer
Model B40 for Grass Amplifier
EC1 73-2694 HA-HSE Isotonic Lever Transducer
Model B40 for Gould 6600 Series
EC1 73-0572 Thread Clip, Clamp for Fixing Thread
housed in the light-proof casing.
The output of the photo transistor is linear over approximately 50° of
movement of the lever. A balance control is provided on the front of the
instrument to zero the output at a convenient level. A long lasting mercury
battery is supplied. Output is from a pair of 4 mm sockets on the front panel.
Specifications
teaching
Battery-Powered Teaching
Isotonic Transducer
This Battery-Powered Teaching Isotonic
Transducer has an output shaft located in precision bearings, which carries a
lever 90 mm (3.5 in) long. This lever is notched on its top surface and pierced
with eight holes on either side of the lever point on a 5 mm pitch from the
fulcrum. These piercings are for attachment of the preparation.
A graduated optical density vane (a light wedge), is also mounted on this shaft
and moves between a miniature lamp and a silicon phototransistor which are
Dimensions:
Case, H x W x D
50 x 100 x 25 mm (2 x 4 x 1 in)
Mounting Rod, OD x L
6.5 x 60 mm (1/4 x 2-1/4 in)
Order #
Product
EC1 50-6378 Battery-Powered Teaching IsotonicTransducer
EC1 50-9257 4 mm plugs to BNC Cable; for use with
Modular Universal Oscillograph, see page 68,
and Student Oscillograph, see page 71
EC1 50-6364 Adapter Cable, 4 mm plugs to 14-Pin DIN
connector; for use with Gould 6600 Series Amplifiers
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10
Isotonic Transducers
transducers
Isotonic Research Transducer
Parameters Measured
• Changes in muscle length under a constant load
Components included
• Transducer
• Specifications Sheet
• Callibration Certificate
research
TRO Isotonic Research
Transducer
The TRO Isotonic Transducer measures
displacements with pre-adjusted loads (position transducer).
Isotonic transducers are classically used for measuring the contraction
amplitude of isolated muscles in Organ Bath studies, i.e. the changes
in tissue length observed under a constant load. These transducers are
preferably used with smooth muscle preparations characterized by
relatively slow contractions (ileum, vas deferens, lung strips, esophagus,
urinary bladder, etc.).
Specifications
Range
5 mg to 5 g
Resolution
5 mg
Displacement
±15 º (48 mm)
Sensitivity (full range)
300 mV
Excitations
5-10V DC
1000 W
Counterweight
graduation
Five 1 g div. with 250 mg subdiv.
Model
EC1 76-0142 TRO015
Harvard Apparatus
Teaching Isotonic
Transducers
This Harvard Apparatus Teaching Isotonic Transducer is exceptionally
sensitive and stable, requiring a minimum of force to produce a
substantial electrical signal. It uses precision ball bearings and a
graduated optical density vane that moves between an infrared source
and a sensor.
The notched, pierced input lever is 90 mm (3-1/2 in) long for easy
connection to muscle tissue. A spring and anchor post are supplied for
converting the unit into a Transducer capable of recording frog/heart
muscle tension for auxotonic applications.
This Transducer has a 2 m (6.6 ft) shielded integral cable which terminates
in a specific connector for use with one of the amplifiers/signal
conditioners below.
Specifications
Output
50 mV/angular degree rotation
Angle of Rotation
±25° above and below horizontal axis
Excitation Voltage
-12, 0, +12 VDC
Breakaway Torque
0.05 g/cm
Dimensions:
Element Resistance
Order #
teaching
Product
Housing, H x W x D
45 x 35 x 45 mm (1.75 x 1.5 x 1.75 in)
Mounting Rod, OD x L
6.5 x 60 mm (0.25 x 2.5 in)
Order #
Product
EC1 50-6360 Harvard Apparatus Teaching Isotonic Transducer
for use with EC1 53-6132 Harvard Apparatus Amplifier,
EC1 50-7970 and EC1 50-7996 Freestanding Transducer
Interfaces, and EC1 50-8861 Modular Transducer Interface
Isotonic Research Transducer
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
11
Introduction to Stimulators
stimulators
HA-HSE Stimulator I Series, see page 15
Digital Stimulator, see page 25
Harvard Apparatus Dual Impedance Research Stimulator,
see page 29
LE 12406 4-Channel Digital Stimulator, see page 28
Biological experiments that involve the functional study of
isolated or in-situ organs or tissues such as nerves and
muscles can only be examined if they are artificial induced
to perform some reproducible activity. Apart from chemical
stimuli, this artificial stimulation used in these experiments
is mainly achieved through the use of electrical stimuli. All
types of instrumentation that are used to accurately
generate these electric stimuli with precise timing and
repeatability can be classified as stimulators.
There are 3 basic components (functions) that are
common to all stimulator systems, Timing, Signal
Amplitude/Quality, and Delivery.
Timing is the basic spacing between each stimulation event
(Frequency/ Rhythm) and the duration of the stimulation
pulses themselves (pulse Width). Stimulation timing can be
as simple as the generation of a single stimulation pulse or
complex, consisting of repetitive pulses or pulse trains of
varying frequency and pulse widths. Timing functions are
either built into a stimulator system or are supplied from
another type of instrument such as a timing generator or
computer. The pulses produced by the stimulator’s timing
generators are usually made up of mono-polar or
bipolar/bi-phasic square waves, although in some
applications such as shock applications where a sinusoidal
waveform is used. Other types of waveforms such as
triangular and trapezoidal pulses are also employed for
other specialized physiological investigations.
Amplitude of the output stimulation is the measure of
stimulation energy intensity that can be provided by the
Quality of the stimulus output. This is usually in the form of
either a constant current or constant voltage but can be other
type of stimuli such as magnetic stimulation. The intensity of
stimulators of the constant current variety would adjust the
amount of current measured in units typically from mA to
Amps. In constant voltage systems this intensity would be
measured in units from mV to hundreds of Volts. Normally
the stimulation pulses are mono-polar going from 0 to either
a positive or negative potential.
When minimal electrophoretic ion transfer is required to
reduce the double layer of gas bubbles that form on
polarized electrode, a system that generates bipolar/biphasic pulses is needed.
Delivery is any of the various methods used to electrically
connect the stimuli to the tissue. Simplistically, the electric
current travels along the cable from the stimulator into the
muscle or nerve through some form of electrodes. These
electrodes are made from various materials that are both
conducting and tissue-compatible. Some of the preferred
electrode materials are platinum, platinum/iridium, silver
and stainless steel although other material such as carbon
fiber are sometimes used.
Harvard Apparatus, Inc. offers a wide variety of stimulator
systems including modular systems for research use, such
as the Stimulator I Series and the Neurolog systems that
can be configured from variety of plug in function modules
or stand-alone stimulators and stimulus isolations units for
dedicated benchtop use. Several models of stimulator
teaching systems are also available. Stimulator functions
are also available in computer controlled multipurpose
physiological measurement systems such as the PLUGSYS
system. There are also a range of stimulation electrodes for
a wide range of applications.
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
12
Heart Stimulating Electrodes
stimulators
EC1 73-0160
Small Stimulation
Electrode Set
EC1 73-0181
Mini Coaxial
Stimulation Electrode
EC1 73-0220
20
EC1 73-0219
75 mm
Coaxial Stimulation Electrode for the
Isolated Heart (Rat, Guinea-pig, Rabbit...)
Electrical stimulation of the isolated heart ensures constant heart rate.
The tissue is being stimulated between the inner wire and the outer
cylinder. Through the coaxial construction, the outer electrode at zero
potential acts as a screen so that stray fields are reduced and interference
to ECG (EG) recordings is limited. A special holder for easy positioning of
the electrode on the heart surface is available as an accessory.
Specifications
Outside Diameter
4.3 mm
Length of Electrode
75 mm
Cable Length
120 mm (4.7 in) ending on 4 mm MC Banana Plugs,
2-core screened cable
Weight
40 g, without holder
Order #
mm
• For electrical
stimulation of the
isolated perfused heart
• Reduced stray fields
for more accurate
recordings
Product
EC1 73-0219 Coaxial Stimulation Electrode
EC1 73-0220 Holder with Maxi-Ball Joints for Large
Stimulation Electrode. Includes 9 mm Ball with
130 mm length and 8 mm diameter Rod (EC1 73-3323),
Maxi-Ball High Load Link (EC1 73-3322), and special bar
with ball and thumbscrew
Mini Coaxial
Stimulation Electrode
for the Isolated Heart
(Mice)
This stimulation electrode set is used for electrical stimulation of small
heart or tissues. Through the coaxial construction the other electrode at
zero potential acts as a screen so that stray fields are reduced and
interference to recordings is limited (ECG, MAP). In combination with the
mini ball joint holders a precise fixation on the myocard is possible.
This electrode can also be used for precise stimulation of small tissues insitu or in horizontal tissue baths like Mayflower see page K43 to K53.
Specifications
OD
1.3 mm
Electrode Length
20 mm
Cable Length
35 cm
Connector
Modified Lemo
Order #
Model
EC1 73-0181 Mini Coaxial Stimulation Electrode with
modified Lemosa connector
EC1 73-0182 Adapter Cable - modified Lemosa connector to
4 mm Banana Plugs, 2 mm Cable Length
EC1 73-0160 Small Stimulation Electrode Set, Includes
EC1 73-0181 & EC1 73-0182
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13
Stimulating Electrodes
Bipolar Stimulating Electrodes for Nerves
stimulators
EC1 73-0337 1.0 mm
Plexiglas Platinum
Electrode
5m
m
EC1 73-0337 1.0 mm
Plexiglas Platinum
Electrode – closeup
4m
m
3m
m
Sliding Jaw
• For in situ applications
• For stimulating or recording
The Plexiglas-platinum electrodes are used for direct stimulation of the
exposed nerve in situ. The sliding jaw is opened for inserting the nerve.
The diameter should fit smoothly without clamping the nerve. The
surgery recess is filled with warmed liquid paraffin to prevent the nerve
drying out. This offers the additional advantage of keeping blood and
saline solution away from the nerve and preventing damage to it. It also
prevents the bipolar electrode from short-circuiting. The electrode
weighs 15 grams (0.5 oz). Those electrodes can also be used for
recording (e.g., Vagus Nerve).
Order #
Product
EC1 73-0336 Plexiglas Platinum Electrode, ID of Closed
Jaw 0.5 mm
EC1 73-0337 Plexiglas Platinum Electrode, ID of Closed
Jaw 1.0 mm
EC1 73-0338 Plexiglas Platinum Electrode, ID of Closed
Jaw 1.5 mm
EC1 73-0339 Plexiglas Platinum Electrode, ID of Closed
Jaw 2.0 mm
Sliding Jaw
EC1 73-0340 Plexiglas Platinum Electrode, ID of Closed
Jaw 3.0 mm
15 mm
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
14
advanced
research
Stimulator I
• Modular rack-mountable construction
• Plug in modules for timing and control,
stimulation output and specialty functions
• Configurations for up to 8 output (stimulation)
channels
• Select from either constant current/constant
voltage, constant voltage only and constant
current only output modules
HA-HSE modular, rack mountable stimulator is designed to meet the needs
of almost every application that requires a controlled electrical
voltage/current stimulus. This system provides a flexible platform that can
be easily configured from a number of basic and specialized plug-in
modules or selected from several pre-configured systems.
The Stimulator I system has a single row of modules consisting of 8 module
slots. The first 4 of these slots are used for the basic timing/control modules
and the other 4 positions are used for output, additional timing/control
and/or other types of specialty function modules. The single rack
configuration of the STIMULATOR I will provide one channel of basic timing
controls (4 module slots) and up to 2 stimulus output modules.
There are a number of plug-in modules that will fit into the slots of the
Stimulator I chassis. The two basic types of stimulator modules, one for
controlling the timing (Timing and control modules) of the stimulus and the
other to produce the stimulation energy as either constant current or
constant voltage (output modules).
stimulators
In addition to the basic timing and output modules (base Rhythm, Delay,
Width and Timer) there are a number of modules for special applications:
• High Current Output Module EC1 73-0086 with
alternating polarity (Bi-Phasic) prevents the
formation of gas bubbles and polarization of the
stimulation electrodes.
• R-Wave Trigger Module EC1 73-0079 provides
electrical stimuli that can be generated in
synchronism with the cardiac rhythm.
• Double Stimulus Preset DP Module EC1 73-0080
permits the production of double stimuli after a
pre-selected number (0 to 99) of single stimuli.
• Stimulus Preset SP Module EC1 73-0081 controls
the number of stimulus pulses produced by an
adjustable number (1 to 9999 pulses) rather then
by time.
For applications that require an isolated (ungrounded) stimulus (e.g. when
applying a stimulation while simultaneously recording biological
potentials), a Universal Isolated Stimulation benchtop unit (EC1 73-0082)
can be used to convert the ungrounded stimuli into low-capacity isolated
stimuli. Although stimulators are mains (AC) operated, use of the isolation
unit produces the same conditions as a battery-operated instrument
providing the stimuli. If you have any questions on how to configure a
system for your specific applications, call our Technical Sales department
for assistance.
The STIMULATOR I model can be equipped with up to output modules.
Output modules are available in one and four stimulation outputs so that
up to eight (8) individual outputs of stimulus can be achieved for
applications such the simultaneous stimulation of isolated tissues. With
the available output modules, systems with 1,2,4,5 or 8 outputs can be
configured.
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
15
stimulators
Stimulator I
advanced
research
EC1 73-0075
EC1 73-0084
To the left is an illustration of the EC1 73-0050 Stimulator I Single Channel
Chassis. It includes the following timing modules:
• EC1 73-0066 Start Mode Module
• EC1 73-0068 Basic Rhythm R Module
• EC1 73-0070 Delay D Module
• EC1 73-0069 Width W Module
Harvard Apparatus offers five pre-configured Stimulator I systems. Each
offers a selection of different modules for different stimulation
applications. By combining the Stimulator I basic timing system with the
various stimulator output and pulse train timing modules, multiple types of
stimulator systems can be configured for a variety of applications.
Additional modules are also available on pages 17 to 21.
EC1 73-0072
EC1 73-0073
Specifications: Stimulator I Rack
Supply
220 V (15%), 50 Hz; 90 VA; can be switched to 110 V
Dimensions, H x W x D
150 x 440 x 355 mm (5.9 x 17.3 x 14 in)
Weight
9 kg (19.8 lb)
Stimulator I Preconfigured Systems
System Order #
System Components
Order #
EC1 73-0051
EC1 73-0052
EC1 73-0053
EC1 73-0054
EC1 73-0055
Basic Stimulator I Chassis
EC1 73-0050
1
1
1
1
1
Single Channel Output OVA
EC1 73-0084
1
1
–
–
–
Train Rhythm TR
EC1 73-0072
–
1
–
–
1
Train Width TW
EC1 73-0073
–
1
–
–
1
4-Channel Voltage Output O4V
EC1 73-0075
–
–
1
2
1
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16
advanced
research
stimulators
HA-HSE Modules for Stimulator I
Start Mode S1 Module
For single-channel stimulators. This module is used in Stimulator I to start
the stimulation in one of the following modes:
• With local switch or remote contact:
— Single stimulus immediate
— Single stimulus delayed
— Twin stimulus immediate
— Repeated stimulus while switch is pushed
repeated stimulus as train
• With trigger pulse
• Free-running
Order #
Product
EC1 73-0066 Start Mode S1 Module
Start Mode S1
Basic Rhythm R Module
This module controls the time spacing of the repeated square-wave pulses.
Specifications
R
Basic Rhythm R
Adjustment Range
0.5 msec to 1000 sec, this corresponds to frequency
range of 2000 Hz to 0.001 Hz
Trigger Output
Positive pulse of 10 V on Ri = 1 kΩ
Gate Input
Inactive +10 V, active 0 V at 10 kΩ input impedance
Order #
Product
EC1 73-0068 Basic Rhythm R Module
Stimulus Width W Module
Controls the duration during which the stimulus is present.
Specifications
W
Width W
Adjustment Range
5 µsec to 100 sec and • = d.c. stimulus; if stimulator is
fitted with delay module, it is possible to select twin
stimuli; stimulus duration indicated by red signal lamp
Trigger Output
Inactive +12 V, active 0 V on 1 kΩ
Trigger Input
Positive rise to +2V on 10 kΩ input impedance
Order #
Product
EC1 73-0069 Stimulus Width W Module
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17
advanced
research
stimulators
HA-HSE Timing and Output Modules for
Stimulator I
Delay D Module
Controls the time spacing from a repeating (first) square-wave pulse to a
subsequent (second) pulse.
D
Delay D
Specifications
Adjustment Range
5 µsec to 100 sec or 0 = no delay, delay time is
indicated by red signal lamp
Trigger Output
Inactive +12 V, active 0 V, Ri = 1 kΩ
Trigger Input
Positive rise +2 V on 10 kΩ internal impedance
Order #
Product
EC1 73-0070 Delay D Module
High-Current Output OLV Module
This module is used in stimulator I for field stimulation of a single isolated
tissue, either at constant voltage or at constant current.
Specifications
Module OLV
Output Range:
Constant Voltage
0 to 30 V in 2 ranges, 0.9 A (DC) or 5 A
(pulse width 1 msec max.)
Constant Current
0 to 3 A in 2 ranges at max. voltage of 30 V
Output Characteristic
Monophasic or alternating to avoid polarization of
stimulation electrodes
Output Connector
Safety female banana jacks
Order #
Product
EC1 73-0086 High-Current Output OLV Module
Output OV Module
constant voltage
10
This module is a simpler version of the OVA module, which provides only a
constant voltage stimulus
Specifications
Constant voltage 0 to 200 V in 3 ranges at source
resistance of less than 10 Ω, current output can be
up to 100 mA (DC operation) or up to 200 mA (pulse
width 1 msec max.)
Output Range
0 - 200 V
Output Characteristic
Monophasic, polarity can be reversed
Output Connector
Safety female banana jacks
Output OV
Order #
Product
EC1 73-0085 Output OV Module
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18
advanced
research
stimulators
HA-HSE Output Modules for Stimulator I
Output OVA Module
constant voltage
constant current
2k
10
This module is used in Stimulator I and controls the amplitude of the
stimulus as constant voltage or constant current.
Specifications
Output Range:
0 - 100 mA
0 - 200 V
Constant Voltage
Constant Current
Output OVA
0 to 200 V in 3 ranges at internal resistance of 10 Ω
currents up to 100 mA (DC) or 200 mA (pulse width
1 msec max.) are produced
0 to 100 mA in 3 ranges at source resistance of 100,
1000 or 10,000 kΩ depending on range, max.
voltage produced is 200 V
Rise Time
Rise speed of stimulus is 10 V per µsec
Output Characteristic
Floating, capacity of 1 nF, monophasic, polarity can
be reversed
Noise
Voltage Monitor Output
U = Io x 50 Ω
Output Connector
Safety female banana jacks
50/60 Hz less than 1 µA
Current Monitor Output
Order #
U = Uo/20
Product
EC1 73-0084 Output OVA Module
4-Channel Voltage Output O4V Module
constant voltage
10
0 - 200 V
Output O4V
This module is used for simultaneous stimulation of 4 isolated tissues, e.g.
in the Schuler or Graz organ baths. Each of the channels provides
constant-voltage stimuli (0 to 100 Volt) adjustable on a 10-turn
potentiometer for each channel. The channels are not isolated from each
other. The large output current permits field stimulation.
Specifications
No. of Outputs
4
Output Range
Constant Voltage 0 to 100 V, max. output current is
40 mA (DC stimulation) or 200 mA (pulses 1 msec max.)
Output Characteristic
Monophasic, polarity can be reversed separately on
each channel
Output Connector
Safety female banana jacks
Order #
Product
EC1 73-0075 4-Channel Voltage Output O4V Module
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19
advanced
research
stimulators
HA-HSE Train Modules for Stimulator I
Automatic Timer Z
Consists of:
A. Train Rhythm TR Module
TR
This module controls the time spacing of the stimulus groups. This module
is used in Stimulator I.
Specifications
Train Rhythm TR
Adjustment Range
5 msec to 1000 sec in 5 switched ranges; single-digit
display indicates time countdown; ON-OFF switch is
fitted
Trigger Output
Positive pulse +10 V on Ri = 1 kΩ
Gate Input
Inactive +10 V, active 0 V, Ri = 10 kΩ
Order #
Product
EC1 73-0072 Train Rhythm TR Module
B. Train Width TW Module
This module controls the time during which the stimuli are switched on.
This module is used in stimulator I.
Specifications
TW
Train Width TW
Adjustment Range
0.5 msec to 100 sec; single-digit display indicates
time countdown; single train can be triggered by
pressing key
Trigger Output
Inactive +12 V, active 0 V, Ri = 1 kΩ
Trigger Input
Positive rise to +2 V at Ri = 10 kΩ
Order #
Product
EC1 73-0073 Train Width (TW) Module
C. Automatic Timer AT1 Module
TR
This module permits the production of stimulus groups (trains), with the
times selected not continuously but in discrete steps. This module is used
in Stimulator I.
Specifications
TW
Auto Time AT1
Train Rhythm (TR)
0.5/1/2/5/10/15/30 sec and 1/2/5/10/20 min
Train Width (TW)
0.1/0.2/0.5/1/2/5/10/30 sec and 1/2/5/10 min
Trigger Output
Inactive +12 V, active 0 V, Ri = 1 kΩ
Trigger Input
Positive rise to +2 V at Ri = 10 kΩ
Stimulus Trains
Single or repeated stimulus trains can be produced
by operating start key
Order #
Product
EC1 73-0074 Automatic Time AT1 Module
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20
stimulators
HA-HSE Special Modules for Stimulator I
advanced
research
HSE
R-Wave Trigger RT Module
Stimulator
R-Wave Trigger RT
This module can be triggered directly from the 1 mV ECG signal. Linking
the trigger output of the RT module to the trigger input of a delay module
D permits e.g. synchronized stimulation of the heart; the stimulation
pulses can be set at any spacing from the R-wave of the ECG, and can be
placed e.g. into the vulnerable phase to produce ventricular fibrillation.
This module has to be inserted in the normal place of TR or TW. Requires
adjustments in wiring of the housing.
10V
1V
Trigger
Output
Blocking
time
TB (x 10ms)
∑D+W+TB
10
15
Off
5
20
2
TB only
4
Gain
6
2
8
0
Control Outp.
R-Wave
1 Volt
10
Polarity
Off
Order #
Product
EC1 73-0079 R-Wave Trigger RT Module
ECG–
Input
1mV
(floating)
Type 215/22
HSE
Double Stimulus Preset DP Module
Stimulator
C
Double Stimulus Preset DP
Aus
This module permits the triggering a second pulse (twin stimulus) after a
certain number of normal single stimuli. If the counter is set to 5, for
example, then an additional second and equal stimulus is produced after
every fifth stimulus. The preset number can be selected between 1 and 99.
Use of this module in Stimulator I requires modification of the basic
stimulator. This module has to be inserted in the normal place of TR or
TW. Requires adjustments in wiring of the housing.
Ein
jeder n-te Reiz wird als
Doppelreiz ausgelost
n=
1 4
Order #
einzeine Doppelreiz–Auslosung
gesteurt von G1 bzw. G2
Product
EC1 73-0080 Double Stimulus Present DP Module
10V
No
1
2
3
13
14
1
2
3
Vorbereitung
Type 215/8
HSE
double stimulus
Stimulus Preset SP Module
Stimulator
Stimulus Preset
Trigger
Output
This module can be used instead of the train width module TW 215/16
and controls the train by the number of pulses instead of by time (width).
Pulse trains with pulse counts between 1 and 9999 can be produced. This
module can be used in Stimulator I at the place of TW. It ensures that no
pulse is ‘cut’ at the end of the timed period.
10V
10V
SP
Preset = n+1
n=
0 0 3 2
Order #
Product
EC1 73-0081 Stimulus Preset SP Module
Single Start
Trigger Input
Pulse No
1
2
3
32
10V
TW
TR
Type 215/24
SP = TW
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21
advanced
research
stimulators
HA-HSE Programmable and Universal
Isolated Stimulators
• Combination of stimulator and
measurement system in one main frame
• Up to 8 channels in one main frame
• For tissue baths or isolated heart
applications
• Generates isolated stimuli in the
range of 0 to 100 Volt
• Can be connected to any
stimulator delivering a
trigger signal
• Can be used in combination with
a trigger output generated by a
computer software as a power
amplifier
• Applications:
– Electrophysiology
– In case of simultaneous
biopotential recording
HA-HSE Programmable Stimulator
Module PSM for PLUGSYS System
This programmable stimulator module is intended for the
stimulation of isolated tissue, with operation from PC
computer. An appropriate stimulation program is available.
The adjustments are made from the PC and transferred to
the PSM. The PSM is ideally suitable for computercontrolled tissue bath setups, e.g. for automatic refractory
period determination. Up to 8 PSM modules can be mounted
inside a PLUGSYS housing see page 31. For more than 4
modules an extra-large power supply is required: Option 5.
Hardware Required:
PC, PLUGSYS housing(603 only) with HA-HSE-data acquisition hardware,
PLUGSYS version and DIM-D digital link.
Software Required:
HA-HSE Stimulator Control Program ‘Stimulus’ or HA-HSE ACAD, ISOHEART,
NEURODYN,
EPES with the
Software
module to
control the PSM
Sample PLUGSYS
Setup: PLUGSYS
Equipment for
Stimulated Isolated
Tissues Module
Specifications
HA-HSE Universal Isolated Stimulator
Output UISO
This unit is required to produce ungrounded (isolated or floating) stimuli
and prevent interference in any biopotentials (ECG, EMG, EEG etc.) which
are recorded simultaneously. It can be connected to virtually any type of
stimulator delivering a trigger pulse with the same width as the stimulus
duration. The input for the trigger impulse is a BNC socket on the back
plane. It can also be connected to a TTL trigger output generated by a
software on a computer as a power amplifier. A switch selects the output
amplitude range (0 to 50, 0 to 100 Volt). Fine adjustment is provided by the
fine control on a 10-turn Potentiometer. Stimuli produced can be either
monopolar (positive or negative), or alternated. Alternated stimulation
avoids electrode polarization.
Specifications
Trigger Signal
3 to 15 V, Trigger level 1.5 V, active low or active high
(can be switched internally using jumper)
Trigger Input Impedance 10 kΩ
Trigger Input Width
Continuous stimulus, trains, triggered from hardware
or software; single or twin stimuli; polarity at output
can be set constant or alternating
10µs to 10 msec defines Stimulus duration; if Trigger
Signal Width exceeds 10 msec, stimuli duration is
limited on 10 msec
Stimuli Output
Constant voltage, floating, isolated
Basic Rhythm
100 msec to 10 sec
Stimuli Output Voltage
Two ranges 0 to 50 V, 0 to 100 V
Delay
1 msec to 1 sec or 0
Stimuli Output Current
Width
0.5 msec to 200 msec
1.8 to 2 A for stimuli duration < 100 µsec, 0.5 A at
10 msec stimuli duration
Output
1 mA to 25 mA or 10 mA to 250 mA, max. 40 V
Stimuli Polarity
Positive, Negative, Alternating
Dimensions, H x W x D
160 x 55 x 250 mm (6.3 x 2.2 x 8.7 in)
Weight
1.3 kg (2.9 lbs)
Trigger Modes
Dimensions, H x W x D
27.5 x 20.2 x 220 mm (101 x 0.8 x 8.7 in)
PLUGSYS Width*
1 slot unit
Operation:
+5 V, 700 mA and +24 V, 500 mA
Weight
0.35 kg (0.8 lb)
*Note: For description of slot unit, see page 31.
Order #
Product
EC1 73-0221 Programmable Stimulator Module PSM for
PLUGSYS System
Order #
Product
EC1 73-2696 Universal Isolated Stimulation Unit,
110 VAC, 60 Hz
EC1 73-0504 Universal Isolated Stimulation Unit,
230 VAC, 50 Hz
EC1 73-3497 Software “Stimulus” for PSM and PPG Control
EC1 73-0224 Software Module to HA-HSE Software for
PSM and PPG Control
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22
advanced
research
stimulators
HA-HSE Stimulator C
•
•
•
•
Digital controls
Precise timing accuracy
Compact
Bright display simplifies setup and view of
all settings
• Monophasic, biphasic and alternating modes
• High current output facilitates field stimulation
The Stimulator C is a compact, highly stable square wave pulse stimulator
that features precise digital timing control. A large LCD display (2 lines at
40 characters) and minimum of controls make setup and operation easy.
All relevant stimulation parameters are shown on the display which
provides a rapid view of the current settings. Several stimulation modes
are possible (repetitive, single, triggered, tetanic...).
Specifications
Stimulation Mode
SINGLE: single stimulus triggered by MANUAL
button or by remote contact
FREE RUN: repeating stimulus
GATED: repeating stimulus triggered by MANUAL
button or by remote contact
TRIGGER: pulse (TTL, negative-going edge) starts
delay circuit
TETANIC: 50 Hz duty cycle square wave. Burst of
2.5 sec maximum duration gated by MANUAL
button or remote contact
Stimulation Timing
Frequency
0.01 to 1000 Hz in 42 coarse steps and and
continous fine setting with 10 µs resolution
respectively
1 ms to 100 s in 41 coarse steps and and continous
fine setting with 10 µs resolution
Delay Time
(leading the stimuli): 0.1 to 500 msec in 32 coarse
steps and and continous fine setting with 10 µs
resolution
Width
10 µsec to 100 msec in 32 coarse steps and
continous fine setting with 2 µs resolution
Stimulus Output
1. Output voltage 0 to 50 V; Current +/- 400 mA (rise
and fall time < 1 µs at 50v +/- 400 mA)
2. Monophasic (Pos or Neg), Biphasic, Alternated
3. Output circuit floating, not connected to earth
(ungrounded)
Indication
1. LCD display (2 lines of 40 characters each with
LED backlight) to indicate stimulus parameters and
settings
2. LED for stimulus pulse
3. Beep (to indicate errors in setting)
Trigger In/Output
BNC socket Input or Ouput depending on the
selected mode.
Related products
Input Signal
TTL level, negative edge or active low
For applications that require an extremely low-capacity isolated stimuli,
the HA-HSE UISO (Universal Isolated Stimulation Output benchtop unit)
can be driven directly by the Stimulator output. This is mainly necessary
when applying a stimulation while simultaneously recording biological
potentials.
Output Signal
TTL level active low
The last settings used are always stored in memory and recalled when
the unit is turned on. Two stimulation pattern can be predefined and
stored in the non volatile memory. These pattern can easily be recalled by
selecting it in the menu.
The basic rhythm can be displayed either as frequency in Hertz or as
period in msec respectively sec. The adjustment of the timing is
controlled by a microcontroller to avoid wrong settings.
The powerful (20 VA) and fast (rise and fall time < 1µs) constant voltage
(up to 50 Volt) output allows stimulation of all types of tissues from direct
contact nerve to tissue bath field stimulation. The output is floating, not
connected to earth (ungrounded). The output has a very low impedance
(< 0.1 Ω). The maximum output current is +/- 400 mA.
Weight
2.5 kg (5.5 lb)
Dimensions, H x W x D
120 x 240 x 250 mm (4.7 x 9.4 x 9.8 in)
Order #
Product
EC1 73-3713 HA-HSE Stimulator C, 220 VAC, 50 Hz
EC1 73-3716 HA-HSE Stimulator C, 115 VAC, 60 Hz
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23
stimulators
HA-HSE Stimulator CS for Isolated Cells
Max. Output Current [A]
advanced
research
5
4
3
2
1
0
0,01
• Easy to use
• Constant voltage monophasic, biphasic or
alternating stimulation (> 1A)
• High output impulse current
• Backup memory for timing setting
• Specially adapted for isolated cells stimulation
0,1
1
Stimulation Width [ms]
10
Specifications
Stimulation Mode
SINGLE/STRT single stimulus triggered by START
switch or by remote contact
REPEAT/STRT repeating stimulus triggered by START
switch or by remote contact
TRIGGER pulse (TTL, negative-going edge) starts
delay circuit; START switch must be in OFF position
Timing:
The stimulator CS Type 223 is a new development of an electronic
stimulator for the electrical stimulation of isolated cells, e.g. myocytes.
Stimulating isolated cells needs a high impulse current to trigger a
maximum of cells independent of the size, shape specially in cell culture
medium having a low resistance. This is of importance in experiment
where the threshold voltage of cell reaction has to be determined. To
reduce electrochemistry effects such as polarization of the electrodes and
double layer of gas bubbles biphasic stimulation with perfect symmetry is
requested. To be able to activate an action potential by depolarizing the
membrane and to avoid further influence of the stimuli on the cell
reaction, short and powerful impulses are required.
Stimulation Mode and Timing are displayed on a LCD display and can
easily be set in a step through mode using the ‘Menu’ button and the
adjust knob. The last setting of the timing is stored in a backup memory
and recalled automatically after switching on the instrument. The Stimulus
Output Voltage is indicated on the built-in digital voltmeter and can be
Adjusted on two potentiometers: “coarse” and “fine” with adjustment in
the range of 0 to 45 V. The output is floating, not connected to earth
(ungrounded). The output has a very low impedance (less than 1 Ω). To
avoid overheating of the output circuit a dynamic current limiter is built in.
It limits the maximum output current relative to the Stimulation Width.
Stimulation
Frequency
0.05 to 100 Hz (in 29 steps) 0.05, 0.1, 0.2, 0.3, 0.4, 0.5,
0.6,0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50,
60, 70, 80, 90, 100 Hz
Delay Time
(leading the stimuli): 2 to 50 msec (in 7 steps) 2, 5, 10,
20, 30, 40, 50 msec
Stimulus Width
10 µsec to 10 msec (in 28 steps) 10, 20, 30, 40, 50, 60,
70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900µsec
1, 2, 3, 4, 5, 6, 7, 8, 9, 10 msec
Stimulus Output
1. Output voltage 0 to 50 V; Pulse Current up to 1 A at
max. pulse width (10 msec)
2. Monophasic, Biphasic, Alternated
3. Output circuit floating, not connected to earth
(ungrounded)
Indication
1. LCD module (4 lines of 16 characters each) to indicate
stimulus parameter settings (except amplitude);
2. Digital voltmeter (DVM) to indicate output voltage
setting range selectable: 0 - 19.99 V <—> 0 - 50.0 V
3. LED for stimulus pulse
4. Beep (with each stimulus pulse), can be switched off
5. 2 LEDs for polarity of next stimulus: + or -
Trigger Input/Output:
Input
BNC socket, input signal starts time delay, TTL level,
negative active edge
Output
BNC socket, signal: delay time, open collector output
(2 kΩ to +5V)
Weight
3.5 kg (7.7 lb)
Dimensions, H x W x D 110 x 300 x 250 mm (4.3 x 11.8 x 9.8 in)
Order #
Product
EC1 73-0485 HA-HSE Stimulator CS, 220 VAC, 50 Hz
EC1 73-2667 HA-HSE Stimulator CS, 115 VAC, 60 Hz
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24
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stimulators
D330 MultiStim System for
Tissue Bath Stimulation
Typical System
Configurations
•
•
•
•
Versatile tissue bath stimulator
Multiple independent channels
Constant current or constant voltage operation
Modular design
Rack and Power Supply
The Rack and Power Supply unit contains a motherboard that distributes
the power, automatically connects the timing signals between the
generators and the stimulators as well as routing the signals of the
stimulating channels to one or more of the D335-Meter modules. They are
fully enclosed to meet laboratory safety standards so that they can be
used on the bench top.
The D330 MultiStim System is a modular and highly versatile multichannel stimulator designed to be used by biomedical scientists who wish
to accurately stimulate a number of low impedance tissue preparations
with different stimulating voltages or currents. The output channels are
fully protected and galvanically isolated from ground so that a single
earthing point can be chosen and loops avoided.
NOTE: As the normal stimulating procedure is short pulses with a longer
‘gap’ the power supplies are rated for a duty cycle that will give at least
500mA continuous ‘rack’ stimulation current. That is 1 A at 50% duty
cycle or 5A at 10% duty cycle for short pulses.
The system provides a choice of modules for either constant voltage
stimulation (up to 100 V or 1 A) or constant current stimulation (up to
500mA from a 100 V source). The D335 - Meter can be fitted into the
system to allow precise voltage or current monitoring.
This unit can hold up to ten independently adjustable stimulation channels
along with the required timing, gating and monitoring units from the range
of available modules.
A selection of timing modules are available to generate pulses, variable in
frequency and width, which can be controlled as bursts using a gating
waveform, variable in repetition rate and duration or pulse count. For ease
of use, most modules have an error indicator to warn of incompatible
settings or an overload condition. Sockets are fitted to allow full external
control and synchronization if required.
The D330 MultiStim System rack/case is available in two sizes. A 19 inch
rack-mountable unit that can house up to ten stimulation channels and a
‘half-width’ bench-top unit capable of taking up to four stimulation
channels along with the Pulse Train Generator and Gating modules.
The D345 PowerStim is an extension of the D330 MultiStim System range,
and provides the controlled high power stimuli from a low impedance
source needed for Field Stimulation of preparations in extra low
impedance baths. It provides up to 80 V at 2.5 A from each of its four
overload protected channels and these outputs can be externally combined
to give two 5A channels or a single 10A.
D337 19-inch Case
Order #
Product
EC1 65-0352 Model D337 19 in. Rack and PSU
Safety Information
To comply with laboratory safety standards, the complete D330-MultiStim
System must be completely enclosed. Therefore we offer matching D3301 and D330-2 Single and Double Blank Front Panels to complete the front
panel aperture. We cannot sell a system with less than a completely
filled front panel.
Order #
Product
EC1 65-0360 Model D330-1 One-Wide Blank
EC1 65-0361 Model D330-2 Two-Wide Blank
The modularity of the system allows a system to be extended or split
between smaller units for two sites.
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25
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stimulators
D330 MultiStim System for
Tissue Bath Stimulation (continued)
D342 2-Channel
Bi-Phasic Switcher
This 2-Channel Bi-Phasic switcher
is for use with a D333H or D343
module. The unit provides the
switching function required to
alternate the polarity of the stimuli
to each of two preparations. It
provides control of stimulus
polarity (Normal, Alternating or
Reverse) independently for each
channel as well as warning of a
stimulus timing rate that is toofast. The control and timing for the
pulses is generated by selecting
other modules from the D330 MultiStim™ range.
It should be noted that the unit
works with mechanical relays that
require a certain time to switch
their polarity. The circuitry is
designed so that the changeover command is taken (independently for
each channel) from the end of each stimulus timing pulse so that the relay
‘moves’ when no stimulus is being applied.
As with all mechanical devices, relays have a finite life. This is quoted as
a minimum of 100,000,000 mechanical operations. If the relay was to
‘move’ whilst a stimulus was presented the contacts would wear-out
much quicker than the mechanics. Therefore, the circuit will detect and
warn the user if the ‘time-between’ stimuli pulses is less than
5 ms. The user must ensure that this never happens.
The module connects to the outputs of a D333H or D343 via 2 mm - 2 mm
leads (supplied). The D333H/343 has the functions and specifications as
defined in its specification part of this manual. Each module is preset for
specific channels whose outputs are to be switched. Normally, the unit
Stimulators and Monitoring
The D330-MultiStim System provides a choice of two different
stimulators. Each has output channels with independent, automatically
reset output protection and indication of error conditions but they differ in
their capabilities.
will be supplied preset for channels 1 and 2 or as required when a full
system is purchased. That is, the timing signals of the D333H/343 and the
D342 will automatically be the same. The channels set is shown at the
top of the module front panel.
Current and Voltage Monitoring
The current and output voltage of each channel can be monitored independently
with the D335 METER as taken from the output of the D333H/343 stimulation
module. This can show either the voltage or current of the selected channel
immediately before the end of the stimulation pulse. The meter will always
read a positive value irrespective of the polarity of the stimulus.
Specifications
Polarity Selector:
Normal
Left hand blue socket will go positive with respect to
associated right hand blue socket
Alt
Polarity of sockets with respect to each other reverse
with each stimuli
Reverse
Right hand blue socket will go positive with respect to
associated left hand blue socket
Amplitude
As set on associated D333H/D343 channel, optional
D335 METER module will display current and voltage
Current
1 A max. (depends on D333H/D343)
Output
Pair of 2 mm sockets (with compliance)
Mechanical Life
100,000,000 (100 Million) operations at 10 Hz
Electrical Life
5,000,000 operations at 200 mA, 200,000 operations at 1
A Note: only applies if stimulus rate is NOT too high
(< 5 ms ISI)
Indicators:
Reverse
Amber LED indicates output pulse is ‘REVERSE’
Too-Fast
Red LED illuminates if ‘off’ time between stimuli is
< 5 msec
Order #
Product
EC1 65-0356 Model D342 Dual Bi-Stim module
(for use with a D333H/343)
D335 Meter
This module allows selection of the channel for monitoring of current or
voltage. This is displayed on a 3-digit display which is ‘held’ until the next
pulse, for ease of reading.
Specifications
The Meter module is a precise method of monitoring voltage or current
without the need of an oscilloscope and more than one can be fitted per rack.
D333H:
D333H Dual Stimulator
D343 Dual Stimulator
0 to 50mA or 0 to 500mA from 100 V supply
A double-width unit with two totally independent constant voltage
stimulation channels. The D333H has an output capability allowing a
maximum output of up to 1 A per channel.
D335 Meter
00.0 to 99.9 V, 0.00 to 9.99 A, 000 to 999 mA
D343 Dual Stimulator
Similar to the D333H but with constant current stimulation control.
0 to 10V or 0 to 100V at 1A max per channel
Rise/Fail Time
Order #
< 5/20 µsec
Product
EC1 65-0349 Model D333H Dual Stimulator
(0 to 100 V, 1 A Max.)
EC1 65-0357 Model D343 Dual Stimulator
(0 to 500 mA, 100 V Max.)
EC1 65-0351 Model D335 Meter Module
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26
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stimulators
D330 MultiStim System for
Tissue Bath Stimulation (continued)
D332T Pulse Timing
D340 Count & Delay
The D330-MultiStim System provides a common timing pulse to all the
stimulating channels in the system unless the D344 is used for external
control of each channel. These modules provide that timing. This provides
the pulse frequency and pulse width for the system. Incorporates 10-turn
controls for greater accuracy.
Provides the method of introducing an ‘extra’ stimulation pulse (or just a
single stimulation pulse timing to occur) at a delay after a particular pulse
within a train. This can be used for Refractory Period measurements. The
D340T (not shown) is the same module but incorporates a 10-turn control
for greater delay accuracy.
Specifications
Specifications
Frequency
0.01 to 1100 Hz
Count
01 to 99
Pulse Width
0.01 to 110 msec
Delay
0.1 to l0 sec
Order #
Order #
Product
Product
EC1 65-0348 Model D332T Pulse, 10-Turn
EC1 65-0354 Model D340 Count and Delay Module
Control and Gate
D344 Remote
Each system must have one of these modules as they provide the routing
for the timing signals and the connectors for external control and
synchronization. They also contain the power switch rod and supply health
indicator.
This module allows complete and independent control of the timing of
each channel using an external timing generator.
D334B Sync
EC1 65-0358 Model D344 Remote Interface Module
Order #
Product
This basic module is not shown but is the same as the left-hand side of
the D341.
D331AT Sync & Gate
This double-width module adds the control of Gating the duration of a
burst of pulses to the basic functions.
D341A Sync & Train
This module is similar to the D331AT but controls the Gating as number of
pulses in a burst.
Specifications
D331AT:
Gate Repetition
0.1 to 110 sec
Gate Duration
0.01 to 11 sec
D341A:
Gate Repetition
0.1 to 990 sec
Number-of-Pulse
in Burst
01 to 99 sec
Order #
Product
EC1 65-0350 Model D334B Sync Module
EC1 65-0347 Model D331AT Sync and Gate, 10-Turn
EC1 65-0355 Model D341A Sync and Train module
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27
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stimulators
LE Series Stimulators
LE 12406 4-Channel Digital Stimulator
Specifications (continued)
Accuracy
≤ 1% for timings, ≤ 1% for output
Sync Out
5 V TTL signal synchronic w/pulse onset
DC
DC level with the set amplitude
External Trigger
TTL signal allowing for the external control
of the pulse onset
Max. Power Output
20 W
Dimensions:
LE 12106
371 x 153 x 292 mm
LE 12206 & 12406
478 x 198 x 292 mm
Power Supply
220/110 V, 50-60 Hz
Basic Stimulator, LE 12006
LE 12006 Basic Stimulator
Number of Channels
1 channel
Pulse:
Frequency
0.01 - 100 Hz
Duration
0.05, 0.1, 0.5, 1, 2, 5 ms
Output:
Key Features
• Pulse and Train formation entirely controlled by
Microprocessor
• Up to 100V/mA constant voltage/current (check
out models)
• Built-in isolated output circuit (not related to ground)
The LE Series of stimulators provides a complete set of precise, compact
and easy-to-use single-, double- or four-channels digital stimulators for
general pharmacology and physiology.
With a wide range of stimulation parameters, these stimulators offer an
excellent and low priced alternative when single pulse and train
stimulation is required. Also a basic stimulator is also offered.
Constant Voltage
0 - 50 V (1 V resolution)
Accuracy
≤ 5% for timings, ≤ 5% for output
Max. Power Output
15 W
Power Supply
220/110 V, 50-60 Hz
Dimensions
286 x 248 x 68 mm
Order #
Model
Product
EC1 76-0055 LE12106VI
Single Channel Digital Stimulator,
100 V or 100 mA output
EC1 76-0056 LE12106150
Single Channel Digital Stimulator,
150 V output
EC1 76-0057 LE12206VI
Double Channel Digital
Stimulator, 100 V or 100 mA output
EC1 76-0058 LE12406OM
Power Unit, 100 V or 100 mA
output,LE12406TC required for use
EC1 76-0059 LE12406TC
Pulse Generator & Mainframe
for up to 4 units LE12406OM
EC1 76-0060 LE12404
Mainframe for up to four LE
12406OM units
EC1 76-0061 LE12006
Basic Stimulator
Specifications
Options
Digital Stimulators (LE 12106, LE 12206 & 12406)
EC1 76-0062 TRS081
Nerve Electrode
Number of Channels:
EC1 76-0063 TRS082
Bipolar Electrode
LE 12106
1 channel
LE 12206
2 channels
LE 12406
4 channels
LE 12006 (Student)
1 channel
Pulse:
Frequency
1 - 99999 p.p.s. (10 KHz)
Duration
1 µs - 9999 ms
Interval
0.1 - 999.9 s
Duration
1 - 9999 ms
Train:
Output:
Constant voltage
0 - 150 V
(LE 12106/150)
(1 V resolution)
Constant Voltage/Current
0 - 100 V/mA (1 V/mA resolution)
Citations
El-Din MM et al. (2007) Peripheral modulation of dopaminergic receptors affects erectile
responses in rats. Basic Clin Pharmacol Toxicol. 100(4):225-32. (LE 12106, rat, Egypt)
De Salvatore G et al. (2004) Functional alterations of mesenteric vascular bed, vas
deferens and intestinal tracts in a rat hindlimb unloading model of microgravity
Autonomic & Autacoid Pharmacology 24(2):45–54. (LE 12106, rat, Italy)
Colabufo NA et al. (2003) A new method for evaluating sigma(2) ligand activity in the
isolated guinea-pig bladder. Naunyn Schmiedebergs Arch Pharmacol. 368(2):106-12 (LE
12106, guinea-pig, Italy).
De Salvatore G et al. (2003) Effects of in vivo treatment with interleukins 1_ and 6 on rat
mesenteric vascular bed reactivity. Autonomic & Autacoid Pharmacology 23 (2):125–131.
(LE 12106, rat, Italy)
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28
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stimulators
6000 Series Stimulators
The 6000 Series of stimulators are ideal units for
conducting teaching and research experiments. The
range, which consists of the 6002 Basic Stimulator,
6012 Dual Pulse Stimulator and the 6020 Dual
Impedance Stimulator, have been designed
specifically to allow complete compatibility between
all 6000 series stimulators. This allows two or more
systems to be connected together to make a
sophisticated multi channel system.
All systems feature variable pulse frequency, 3
operating modes, variable pulse width, variable pulse
amplitude, an output on or off selector, pulse indicator,
gate in and gate out terminals. The 6012 and 6020
Stimulators also feature a sync output terminal. The
• Single Pulse, Single Channel
• 50 Volt Pulse Amplitude
• 6 Pulse Widths
6002 Basic Stimulator
The 6002 stimulator features a highly stable pulse generator that is
continuously adjustable between 0.1Hz to 100Hz, with a variable pulse
width that can be set to one of six positions and a maximum output
amplitude of 50 Volts.
Specifications
Frequency Range
0.1 to 100 Hz in 3 ranges
Pulse Width Range
0.1 to 5 mS
Output Amplitude
0 to 0.5 Volts, 0 to 5 Volts or 0 to 50 Volts
Rise and Fall Time
> 5 µsec
Gate In
Short Circuit or 12 Volt (Max) Logic Pulse (triggers on
falling edge)
Connections
2 x insulated 4 mm sockets (Output)
2 x BNC Socket (Gate Connections)
Power
Order #
110-115 Volts 60 Hz or 220-230 Volts 50 Hz
Product
EC1 50-6002 6002 Basic Stimulator
6020 stimulator featurers a delay circuit that allows the
output pulse to be delayed in relation to the sync
output for oscilloscope timebase to be pre-triggering.
In continuous mode, systems generate a continuous
stream of pulses, at the selected frequency, amplitude
and pulse width.
In single shot mode, a single pulse is generated at the
selected amplitude and pulse width, whenever the single
shot key is activated or when the gate in condition is met.
In gate in mode, whenever the gate in condition is
met or the single shot key is activated, pulses are
produced at the selected frequency, amplitude and
pulse width, for as long as the condition remains met.
•
•
•
•
•
Dual Pulse Single Channel
Sync Output
50 Volt Output Amplitude
6 Pulse Widths
Produces Tetanic Train Pulses
6012 Dual Pulse Stimulator
The 6012 stimulator is similar to the 6002, however this unit has a wider
frequency range of 0.1 to 1000Hz and a dual pulse function. When dual
pulse mode is selected a secondary pulse is generated behind the initial
pulse; a delay circuit controls the time difference between the two
pulses. The 6012 is also capable of producing Tetanic Train Pulses
internally without the need to utilise any other external triggering.
Specifications
Frequency Range
0.1 to 1000 Hz in 4 ranges
Pulse Width Range
0.1 to 5mS in 6 preset steps
Dual Pulse Delay
0 to 1000 mS
Output Amplitude
0 to 0.5 Volts, 0 to 5 Volts or 0 to 50 Volts
Rise and Fall Time
> 5 µsec
Gate In
Short Circuit or 12 Volt (Max) Logic Pulse
(triggers on falling edge)
Connections
2 x insulated 4 mm sockets (Output)
3 x BNC Socket (Gate & Sync Connections)
Power
110-115 Volts 60Hz or 220-230 Volts 50Hz
Order #
Product
EC1 50-6012 6012 Dual Pulse Stimulator
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29
research
•
•
•
•
stimulators
6000 Series Stimulators (continued)
Dual Output Impedance
Sync Output
150 Volt Output Amplitude
9 Pulse Widths
6020 Dual Impedance Stimulator
The 6020 Stimulator is similar to the 6012 stimulator, however there is no
dual pulse function. Instead this unit has a fully earth isolated output
amplitude of 150 Volts, which can be set at 100 Ωs or 100 K Ωs
impedance. The delay circuit allows the output pulse to be delayed in
relation to the sync output.
Specifications
Frequency Range
0.1 to 1000 Hz in 4 ranges
Pulse Width Range
0.1 to 50 mS in 9 preset steps
Dual Pulse Delay
0 to 1000 mS
Output Amplitude
0 to 0.15 Volts, 0 to 5 Volts or 0 to 50 Volts
Rise and Fall Time
> 5 µsec
Gate In
Short Circuit or 12 Volt (Max) Logic Pulse (triggers on
falling edge)
Connections
2 x insulated 4 mm sockets (Output)
1 x uninsulated 4mm earth connection
3 x BNC socket (Gate & Sync Connections)
Power
110-115 Volts 60 Hz or 220-230 Volts 50 Hz
• Essential safety device for experiments involving
AC-powered stimulators
• Effectively isolates any stimulator having a
minimum output of 12 volts
• Available with positive or negative output pulses
Harvard Apparatus Stimulus Isolation Unit
This Isolation Unit makes true biphasic stimulation available to the
researcher at a low cost when used in conjunction with the EC1 50-6002
Harvard Apparatus Single Pulse Stimulator and EC1 50-6012 Harvard
Apparatus Single/Dual Pulse Stimulator, see page 29. This self-contained,
battery-powered Isolation Unit is constructed of plastic for additional
isolation. The Unit’s output faithfully reproduces the pulse shape
characteristics of the driving stimulator, but is optically isolated from it.
The output voltage is controlled by a 3 decade digital potentiometer
providing a range from zero volts to approximately 96% battery supply
voltage in steps of 0.1%.
In operation, the stimulator controls are set to the required pulse width
and frequency. These factors are maintained independently of the
stimulator voltage. The output of the stimulator is set to 12 volts. It is then
connected to the Stimulus Isolation Unit. The output of this Isolation Unit
is then set to the required voltage and connected to the appropriate
electrodes.
Specifications
Order #
Product
EC1 50-6020 6020 Dual Impedance Stimulator
External Power Source
12 to 100 VDC batteries
Input from Stimulator
100 Ω
Output Impedance
12 V
Dimensions, H x W x D
60 x 155 x 95 mm (25 x 6 x 35 in)
Weight
225 g (1/2 lb)
Order #
Product
EC1 50-8275 Harvard Apparatus Stimulus Isolation Unit* with
Positive Output Pulses
EC1 50-0090 Harvard Apparatus Stimulus Isolation Unit* with
Negative Output Pulses
*Note: This Unit MUST NOT be, under any circumstances, powered by any AC power unit in
place of the batteries. To do so would destroy all the isolation characteristics.
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
30
advanced
research
PLUGSYS
HA-HSE PLUGSYS Modular Measuring
& Control System
• Universal modular measuring and controlling
system for recording and data acquisition
• Wide range of modules
– Transducer amplifiers (force, displacement,
pressure, flow)
– Biopotential amplifiers (ECG, EEG, EMG, AP, MAP)
– Amplifiers for biochemical sensors (O2, pH,
ion selective)
– Calculating modules (differentiator, integrator,
heart rate)
– I/O modules (trigger output, recorder output,
trigger input, signal input)
– Controlling and special function modules
(ventilator module, liquid level controller,
programmable stimulator module)
– Interface to recording devices, data acquisition
and controlling
• Modular structure permits multiapplication
platform use:
– Hemodynamics
– Pulmonary
– Isolated organ
– Biopotentials
– Combinations (hemodynamics and pulmonary)
• Modules can be interconnected internally. There is
no need for external cables which reduces clutter
and confusion. The same is true for recorder and
data acquisition connections, simple, clutter free
connections reduce the chance of error.
General System Description
PLUGSYS is a flexible measuring and control system developed for use in
physiological and pharmacological research. Its modular design accepts a
wide pallet of different plug-in function modules such as bridge amplifier,
differentiator, integrator, heart rate meter, ECG amplifier etc. All these
modules fit directly into the PLUGSYS main frame, which is available in a
19 inch rack or as a desktop case.
The PLUGSYS main frame is the base of the measuring system. It consists of
the case, the standard power supply, and the bus system. The system has 20
slots for PLUGSYS modules, but the maximum number of installable modules
in practice depends on the width of the module. The PLUGSYS configuration
for most applications will result in a maximum of 10 to 15 modules per case.
One of the leading features of the PLUGSYS measuring system is the slotindependent design, that means that all configuration settings are done on
the plug-in modules and not on the system bus. The system can be
modified or reconfigured by the using jumpers and dip switches installed
on the PLUGSYS modules.
The PLUGSYS is not only a conventional modular analog measuring
system, it includes specific interface modules and application software for
data acquisition and analysis.
HA-HSE PLUGSYS System Cases and Minicase
The basic system cases with internal system bus are supplied completely
wired and ready for use. The different models have either 6, 10 or 20
positions (slot units = SU) which accept the PLUGSYS modules. A
MiniCase is also available that has 4 slot units and with an optional Case
Extension that increases the number to 6 slot units.
The Type 601 cases provides a total space of 10 slot units. The Type 603
cases provides a total space of 20 slot units. The width of the function
PLUGSYS modules equals either one or a multiple of it slot units. For
instance, the width of the CFBA Bridge Amplifier is equal to two slot units,
therefore it would occupy 2 slot spaces in the PLUGSYS case. The maximum
number of PLUGSYS modules — such as a Bridge Amplifier, Differentiator,
Integrator, or Heart Rate Module — which can be plugged into a unit
system case depends on the number of slot units required by each module.
The MiniCase housing is a low-cost and space-saving alternative for 1 or 2
modules and allows PLUGSYS modules to be used as conventional standalone units. Unlike the PLUGSYS housings Type 601 and 603, the MiniCase
has no system bus for power supply or signal link between the individual
modules. For this reason, the MiniCase systems are supplied custom wired
and configured to suit your requirements. The MiniCase is only suitable for
use with certain modules or module combinations. Please contact Harvard
Apparatus for more information.
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
31
advanced
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PLUGSYS
HA-HSE PLUGSYS Modular Measuring
and Control System (continued)
EC1 73-0045 Model 603 PLUGSYS Main Frame Case
PLUGSYS Bus
Trigger Bus
Each of the PLUGSYS Mainframe cases has a series of multifunction
connectors into which PLUGSYS modules are inserted. The connector pins
interface with the PLUGSYS bus. The PLUGSYS bus provides the power for
the PLUGSYS modules and is further subdivided into:
The trigger bus is used by some PLUGSYS modules for outgoing and
incoming trigger signals (e.g. synchronization signals between master and
slave modules (see example below). Jumpers on the PLUGSYS modules
are used to assign trigger lines, similar to assigning analog lines. External
(user) access to the trigger bus is provided by the EC1 73-1633 ROM-T
PLUGSYS, see page 55. The following are examples of setups which use
trigger signals:
• A 16-line analog bus (AV1 to AV16)
• A 4-line trigger bus (TR1 to TR4)
• An 8-bit digital bus
Analog Bus
Each PLUGSYS module which produces an analog signal is assigned it’s
own analog channel (AV1- AV16) using jumpers on the module PC board.
Some modules use as their input analog signals from other modules (e.g.
Differentiator module, Heart Rate module, etc.). Using jumpers on the
PLUGSYS module, the input signals can be easily assigned to an AV
channel without the use of cables. The following is an example of a setup
which uses analog input and output signals:
• The PLUGSYS bridge amplifier module TAM-A is
used to amplify and condition a left ventricular
pressure signal. The TAM-A analog output is
assigned by jumpers to line AV1.
• The PLUGSYS Differentiator module is used to
derive dP/dt from the LVP Signal. The input is
linked to the LVP signal on line AV1. The output is
linked to line AV2.
• A PLUGSYS Heart Rate module is used to calculate
heart rate from the LVP signal on line AV1. The
input for the LVP signal is assigned to AV1 and the
output for heart rate signal is assigned to line AV3,
using jumpers on the Heart Rate module.
Each analog signal produced by a PLUGSYS module can be picked up from
the BNC socket on the front of the module. There are two ways to acquire
signals which minimize the potential for cable clutter. The first uses the
EC1 73-0161 Data Acquisition Hardware, PLUGSYS Version, see page 81,
along with HA-HSE* data acquisition software. The second method used
to acquire data from any other data acquisition system or strip chart
recorder, picks up the analog signals using the EC1 73-1684 Recording
Output Standard Set, see page 54.
Setup for Isolated Perfused Lung, see the Isolated Organ Section K.
• The EC1 73-0021 PLUGSYS Programmable
Stimulator Module PSM, see page 65, is used for
electrical stimulation of isolated tissues. If a
multiple channel Stimulator using several PSM
modules is used, these modules can be
synchronized. One module acts as a master
module and the other module as the slave. The
master module Synchro-Output is assigned to TR1 and the slave module Synchro-Input is assigned
to TR-1.
• The EC1 73-1741 PLUGSYS Ventilation Control
Module, see page 58, is used to generate negative
ventilation pressure in the thoracal chamber. A
sigh breath can be inserted in the respiratory cycle
using a EC1 73-1750 Timer Counter Module, see
page 58, to trigger the Ventilation Control module.
The output from the Timer Counter Module and
the ‘DEEP INSP.IN’ from the Ventilation Control
module are both assigned to line TR2. The Timer
module is now set to trigger a Ventilation Control
module sigh breath.
Digital Bus
The digital bus consists of a proprietary 8 bit parallel data bus and several
handshake lines. It is used to control modules such as the EC1 73-0221
Programmable Stimulator or the EC1 73-1748 Plethysmograph Control Unit
which requires control from HA-HSE* software. The EC1 73-0161 Data
Acquisition Hardware, PLUGSYS Version, see page 61 with the DIM-D
option are used to access the digital bus signals.
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32
PLUGSYS
HA-HSE PLUGSYS Cases
advanced
research
MiniCase EC1 73-1523
with Case Extension
EC1 73-1537 and
PLUGSYS Module
TAM-A
EC1 73-0045 Model 603 PLUGSYS Main Frame Case
EC1 73-1521 Model 601 with 5 PLUGSYS Modules
Specifications
PLUGSYS Case Type 603
PLUGSYS Case Type 601
PLUGSYS MiniCase Type 609
Housing Construction
Metallic housing, gray plastic coated,
Universal 19 inch case with pivoting feet,
prepared for rack mounting
Metallic housing, gray plastic coated
with pivoting feet
ABS plastics, flame-resistant to UL94VO,
color RAL 7032 (stone gray), with ventilation
slots at sides
Dimensions, H x W x D
132 x 483 x 435mm (5.2 x 19 x 17.1 in)
150 x 235 x 420mm (5.9 x 9.25 x 16.5 in)
160 x 160 x 250mm (6.3 x 6.3 x 9.8 in);
Option 01 = 206mm (8.1 in)
Weight
6.5 kg (14.3 lbs)
4.8 kg (10.6 lbs) without PLUGSYS modules
1.4 kg (3.1 lbs) approx. without PLUGSYS modules
Power
100 to 240 VAC, 50/60 Hz, built-in line filter,
100 W max. consumption
85 to 264 VAC (40 Watts), output voltage
85 to 264 VAC (40 Watts), output voltage +5 VDC
+5 VDC (5 A) and ±12 VDC (0.5 A) for supplying (5 A) and ±12 VDC (0.5 A) for supplying
PLUGSYS modules
PLUGSYS modules
Accessories
Line Cable, Spare Fuses, Screw driver
for fitting Function Modules, Document
Folder for Operating Instructions of
Function Modules
3 x BNC-BNC Output lines
Line Cable, Screw driver for fitting Function
Modules, Document Folder for Operating
Instructions of Function Modules
3 x BNC-BNC Output lines
Mains Supply Cable and Operating Instructions
2 x BNC-BNC Output lines (3 if with case
extension Option01)
Module Positions
20 slot units max.
10 slot units max.
4 slot units max. without extension case;
possible to fit 6 slot units using internal straps,
Option 1 case extension
Socket Combination
3-pin IEC mains with fuse and mains switch
on back of housing
3-pin IEC mains with fuse and mains switch
on back of housing
3-pin IEC mains with fuse and mains switch
on back of housing
Order #
Product
Order #
Product
PLUGSYS Case Type 603
PLUGSYS Minicase Type 609 (continued)
EC1 73-0045 PLUGSYS Case, Type 603
EC1 73-1537 Option 1, Case Extension: Case width extension
through internal straps, extra width 2 slot unit
case can take front panels up to 6 slot units
EC1 73-1529 Additional Power Supply, Type A
PLUGSYS Case Type 601
EC1 73-1521 PLUGSYS Case, Type 601
EC1 73-3103 Option for Power Supply for PLUGSYS Case
Type 601, +24 Volt (1.6A)
EC1 73-3082 PLUGSYS Bus Adapter to Mount Backside
Modules on the Front
PLUGSYS Minicase Type 609
EC1 73-1523 PLUGSYS Minicase, Type 609
EC1 73-1541 Option 4, 4 BNC Sockets on Rear,
for signal inputs or outputs
EC1 73-1542 Option 5, Housing without Power Supply,
empty MiniCase housing without power supply
and mains input, to take non-electrical PLUGSYS
modules, e.g. Pressure Regulator Module PRM Type
671/3; reduces housing width to 105 mm
EC1 73-3346 Option 7, 6 BNC Sockets on Rear,
for Signal Inputs or Outputs
EC1 73-1538 Option 2, Additional Power Supply, additional +24 V
(1.6 A) output increases max. consumption to 80 W
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33
PLUGSYS
PLUGSYS Bus Link and Adapter
advanced
research
• To mount backside modules on the front side of
the system case
Contact
Us
Two PLUGSYS
Basic System
Cases linked
together with BLM
HA-HSE PLUGSYS Bus Adapter
The PLUGSYS Bus Adapter is used to mount modules that would normally
mount on the backside of the PLUGSYS system case, on the frontside. This
adapter is especially required for the Type 601 Case (EC1 73-1521) to use
the HA-HSE Data Acquisition Hardware for PLUGSYS cases (EC1 73-0161).
• To link together 2 PLUGSYS main frame to
increase module spaces
Specifications
PLUGSYS Width*
HA-HSE Bus Link Module (BLM)
The Bus Link Module is used to increase the number of slot units available by
linking two PLUGSYS mainframe together. This is necessary if the maximum
number of module or the maximum power consumption of one case is
exceeded. The resulting combination of two cases works as one unit. The
BLM does not increase the maximum number of 16 analog (AV) or digital (DV)
connection lines of the PLUGSYS bus system. The BLM itself does not need
any slot space, it is installed on the rear of each PLUGSYS case.
2 slot units
* Note: For description of Slot Unit, see page 31.
Order #
Product
EC1 73-3082 PLUGSYS Bus Adapter
A complete BLM connection kit consists of the following:
• 2 BLM Type 680
• 1 Connection Cable
• 1 Mounting Kit
• 1 Operating Instructions
Note: The BLM module can only be installed in the PLUGSYS basic system case, Type 603.
Specifications
Dimensions, H x W
Rear Panel Installation Space: 128.7 x 40.5 mm
PLUGSYS Width*
2 slot units
Weight
800 g (set of 2 BLM and 1 connecting cable)
* Note: For description of Slot Unit, see page 31.
Order #
Product
EC1 73-0163 BLM Bus Link Module
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34
advanced
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EC1 73-1602 Carrier Frequency
Bridge Amplifier Module CFBA
Order #
PLUGSYS
Summary of HA-HSE PLUGSYS Modules
EC1 73-0149 ECG Amplifier
Module ECGA
PLUGSYS Module Description
Page
EC1 73-0163
BLM Bus Link Module
34
EC1 73-3082
Bus Adapter Module
34
Transducer Amplifiers
EC1 73-0210 Oxygen Partial
Pressure Module
PLUGSYS Module Description
Page
DIF Differentiator Module
52
EC1 73-1587
DIF/S Differentiator Module without
Max/Min Storage
52
EC1 73-1583
INT Integrator Module
52
Input/Output Modules
EC1 73-0065
TAM-A Analog Transducer Amplifier Module
36
EC1 73-1793
TAM-D Digital Transducer Amplifier Module
36
EC1 73-0907
CFAB Carrier Frequency Adapter Box
36
EC1 73-1602
CFBA Carrier Frequency Bridge Amplifier
37
Flowmeters
TTFM Transit Time Ultrasonic Flowmeter Module
Order #
Contact
EC1 73-0164
Us
Amplifier Case Modification Modules
EC1 73-0146
EC1 73-0146 Transit Time
Flowmeter Module TTFM
37
Biopotential Amplifiers
EC1 73-0222
EIM External Input Module (Analog Only)
53
EC1 73-1601
EIM-S Suppression Module for EIM
53
EC1 73-2823
DIM Data Interface Module
53
EC1 73-0223
DIM-D Digital Bus Link for DIM
53
EC1 73-1683
ROM-DL Recording Output Module - Direct Link
53
EC1 73-1684
ROM-A Recording Output Module - Adjustable
54
EC1 73-1639
ROM-C Recording Output Module - Connector
54
EC1 73-0149
ECGA Electrocardiogram ECG Amplifier Module
38
EC1 73-0166
ROM-SET Recording Output Standard Set
54
EC1 73-1753
RWT R-Wave Trigger Module
38
EC1 73-1633
ROM-T Trigger Input/Output Module
55
EC1 73-1778
EGM Einthoven Golberger Module
39
EC1 73-1631
ROM-B 8-Fold BNC Output Module, Signals 1-8
55
EC1 73-1779
WLA Wilson Lead Amplifier
39
EC1 73-1632
ROM-B 8-Fold BNC Output Module, Signals 9-16
55
EC1 73-0153
BPA Biopotential Amplifier Module
40
EC1 73-1752
STOM Software Trigger Output Module STOM
55
EC1 73-1780
MAPM 6 Channel Monophasic Action Potential
Module
40
Controlling Modules
EC1 73-3080
MAPM 3 Channel Monophasic Action Potential
Module
40
EC1 73-1773
MEA Microelectrode Amplifier Module
EC1 73-2806
SCP Servo Controller for Perfusion
56
EC1 73-0195
PRM Pressure Regulator Module
57
45
EC1 73-1647
GSM Gas Select Module
57
EC1 73-1741
VCM-P Ventilation Control Module using
Integral Pump
58
EC1 73-2795
VCM-R Ventilation Control Module using
Pressure Regulated Gas Source
58
58
EC1 73-2665
Headstage PreAmplifier
45
EC1 73-1766
EMGA Electromyogram EMG Amplifier Module
46
EC1 73-1770
EEGA Electroencephalogram EEG Amplifier Module 46
EC1 73-1792
TCAM Thermocouple Amplifier Module
47
EC1 73-1750
TCM Timer Counter Module
EC1 73-1743
PHDA Peak Height Detector Amplifier Module
48
EC1 73-1748
PCU-1 Plethysmograph Control Unit Module
59
EC1 73-1749
PCU-2/4 Plethysmograph Control Unit Module
59
Amperometric and Electrometer Amplifiers
EC1 73-0215
pHMM pH Measurement Module
49
EC1 73-1754
PCU-3 Plethysmograph Control Unit Module
59
EC1 73-1776
pHCM pH Control Module
49
EC1 73-0046
VSM Ventilator Sequencer Module
60
EC1 73-0210
OPPM Oxygen Partial Pressure Module
50
EC1 73-0221
PSM Programmable Stimulator Module
61
EC1 73-0212
EMM Electrometer Module
50
EC1 73-3097
PPG Programmable Pulse Generator Module
62
Calculating Modules
EC1 73-0165
HRM Heart Rate and ECG Amplifier Module
51
EC1 73-1747
RRM Respiration Rate Module
51
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
35
advanced
research
PLUGSYS
HA-HSE PLUGSYS Modules
Transducer
Amplifier Modules
(TAM-A and TAM-D)
The Transducer Amplifier Module
is a DC amplifier with bridge. It is
used to amplify physiological
signals like blood pressure,
contraction force and contraction
EC1 73-0907
displacement using transducers
Carrier Frequency Adapter Box, Type 376
based on a resistive Wheatstone
bridge. Transducers with a built-in
preamplifier which have a high
• Sine wave carrier demodulator for bridge amplifiers
level DC output voltage can also
• Provides a carrier frequency excitation for
be connected. Choose from two:
inductive or capacitive transducers
TAM-A (analog) & TAM-D (digital
• Interfaces differential pressure transducers to
display). The TAM-A is equipped with an analog LED bar graph signal
various amplifiers
indicator and is best suited for applications which require the monitoring
of dynamic signals, e.g. blood pressure. The TAM-D has a digital numeric
display and is best suited for applications with slowly changing signals,
Carrier Frequency Adapter Box,
e.g. isometric or isotonic contractions, intracranial pressure or venous Contact Type 376 (CFAB)
Us
blood pressure.
The Type 376 carrier frequency adapter box (CFAB) allows the interconnection
of an inductive or capacitive transducer (half bridge system) to a DC-bridge
Specifications
amplifier. This adapter box can be used with most differential pressure
Bridge Supply Voltage
+5V / 50 mA max.
transducers and with the HA-HSE lung weight transducer. A DC bridge
Transducer Input
6-pin socket with screw lock (binder, Amphenol Tuchel)
amplifier supplies power, through a 5 volt excitation voltage to the adapter
Differential input circuit, input impedance 1010 Ω
box which in turn supplies a 0.6 volt, 5 kHz sine wave excitation signal to the
Gain
Selectable Ranges by Internal Jumper: 0.2 to 10, 0.4 to
transducer. The transducer signal voltage is demodulated and passed through
20, 1 to 50, 2 to 100, 4 to 200, 10 to 500, 20 to 1000, 100
to 5000, 200 to 10000. Fine Adjustment Through 10-Turn
the adapter box to the DC bridge amp. It is available with connectors for one
Trimmer
of five different amplifiers including both PLUGSYS TAM modules, see
Bridge Balance
Through 10-turn trimmer coarse adjustment and
left column.
electronic autozero by push button (LED for error if
autozero is not possible)
Signal Output
a) On front panel through BNC socket ±10 V pulsatile
filtered or mean signal output internally selectable
b) Through bus connector to PLUGSYS measuring
system through links ±10 V pulsatile filtered and
mean signal voltage
Output Low-Pass Filter
Analog Indication
Electrical Calibration
Specifications
Power Supply
From Preamp Excitation, 5 V/ 30 mA
Transducer Excitation
Voltage
0.6 V, 5 kHz
a) Selectable by switch on front panel for pulsatile
output signal: 1, 100, 300 Hz
b) Selectable by internal jumper for mean output
signal: 0.1, 0.3 Hz
Input Voltage From
Transducer
0 to 50 mV
Gain
40 Ω
TAM-A LED bar graph 20 LEDs (+13/-7) for visual
check on the signal sensitivity approx. 1 V/LED TAM-D
3 1/2-digit LED display
Thermal Zero Shift
< 0.01 mV/°K
Thermal Gain Shift
300 Ω
Selectable by switch on front panel:
a) 0 V output signal with switch in position ‘0’
b) Positive or negative calibration output voltage
adjustable with 10-turn trimmer if switch is in
position ‘CAL’
Power Supply
+5 V through connector from PLUGSYS bus system
PLUGSYS Width
2 slot units
Input Resistance
Output Resistance
Frequency Range
Product
< 0.01 %/°K
0 to 200 Hz, ±5%
Dimensions, H x W x D
40 x 120 x 65 mm (1.6 x 4.7 x 2.6 in)
Cable Length
1.8 m (5.9 ft)
Weight
0.2 kg (7 oz)
Order #
Order #
1-fold
Product
EC1 73-0907 CFAB for PLUGSYS Amplifier
EC1 73-0065 Transducer Amplifier Module TAM-A
EC1 73-2701 CFAB for Harvard Apparatus Transducer Amplifier
EC1 73-1793 Transducer Amplifier Module TAM-D
EC1 73-2702 CFAB for Grass Amplifier, 6 pin Cannon Connector
EC1 73-2703 CFAB for Gould Series 6600 Amplifier,
14 pin Connector
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
36
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