System Microscope
BX63/BX53
BX3 Series
A Clear Edge in Imaging
Images are courtesy of:
Dr. Shigeo Hayashi, Dr. Kagayaki Kato, Dr. Reiko Tajiri and Mr. Hosei Wada
Laboratory for Morphogenetic Signaling
RIKEN Center for Developmental Biology (P.12, P.15 top right, P.16 top left)
Shigenobu Yonemura, Ph.D.
Electron Microscope Laboratory
RIKEN Center for Developmental Biology
(P.6 lower right, P.7, P.15 lower right, P.17 Phase contrast, P.19 top right)
Guojun Sheng, Ph.D., Yukiko Nakaya, Ph.D.
Laboratory for Early Embryogenesis
RIKEN Center for Developmental Biology (P.2, P.15 lower left, P.16 lower left)
Fumio Matsuzaki, Ph.D., Daijiro Konno, Ph.D.
Laboratory
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• OLYMPUS CORPORATION is ISO14001 certified.
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• Illumination devices for microscope have suggested lifetimes.
Periodic inspections are required. Please visit our web site for details.
• All company and product names are registered trademarks and/or trademarks of their respective
owners.
• Images on the PC monitors are simulated.
• Specifications and appearances are subject to change without any notice or obligation on the part of
the manufacturer.
Printed in Japan M1697E-0310B
A Revolutionary New Standard
in Accuracy and Imaging Efficiency
User-friendly design assures outstanding operating ease and flexibility for
brightfield/darkfield and fluorescence imaging in a wide range of research
settings. With a choice of models and configuration options, there's an
Olympus research microscope to meet your individual needs.
1
2
Advanced Sensitivity in Fluorescence Imaging
Accuracy & Ease of Use
New Fluorescence Illuminators
with Fly’s-eye Lens
Fluorescence illuminators BX3-RFAA and
BX3-RFAS equipped with a Fly’s-eye lens
provide uniform illumination. Even, uniform
fluorescence illumination facilitates postimaging enhancements and processing.
Accuracy
DM595
BA600690MCHE
High-sensitivity fluorescence
detection enables bright, high-contrast imaging
with minimal exposure
of cells to excitation light. Advanced Olympus
BP565585MCHE
technologies also
reduce stray light and autofluorescence
to assure a high S/N ratio.
Surface of Fly’s-eye Lens,
Enlarged Image
Transmittance (%)
U-FUW
100
90
80
70
60
50
40
30
20
10
0
300
DM410
BA420IF
BP340390
350
400
450
500
Wavelength (nm)
550
600
Transmittance (%)
U-FMCHE
100
90
80
70
60
50
40
30
20
10
0
500
DM595
BA600690MCHE
BP565585MCHE
Fluorescent Mirror Units with
the Latest Coating Technology
Olympus applies an outstanding filter
coating technology to all fluorescence
mirror units in order to produce high
transmissions, sharp cut-offs and efficient
detections of fluorescence.
550
600
650
700
Wavelength (nm)
750
800
Light
source
Emission filter
Dichromatic
mirror
DM410
Excitation filter
BA420IF
Excitation light:
Illumination light
Stray light
Low reflection
function
Objective
Specimen
BP340390
3
Motorized Sensors with Power-off
Functions
The sensor for the motorized fluorescence
illuminator BX3-RFAA turns the light on only
upon mirror unit changeover commands,
and turns it off as soon as the command is
completed. This function assures that stray
light from the sensor is terminated during
observation.
Fluorescence light
for observation
Stray Light Reduction Equipped on
All Mirror Units
The low reflection function eliminates over
99% of stray light, producing a high S/N
ratio.
High Transmission Objectives with
Reduced Autofluorescence
Olympus UIS2 objectives are made with
superior low-autofluorescence glass, antireflection coats, and lens joining materials,
improving the S/N ratio. Effective detections
of subtle fluorescence emissions even with
weak excitation light deliver ideal
performance in fluorescence imaging.
Condenser Design to Reduce
Back-reflections
The motorized universal condenser is
designed to reduce back-reflections and
autofluorescence by swinging its top lens
out, automatically closing its diaphragm to
the minimum, and locating the wheel in
between two positions.
Low Autofluorescence Immersion Oil
This immersion oil is
specifically designed
to reduce the
autofluorescence
normally associated
with such oils, for
improved S/N ratio. Insusceptible to
crystallization, it is an ideal oil for long time
observations.
4
Reliability and Precision in an Easy-to-use Package
Accuracy & Ease of Use
Upgraded Accuracy
The BX63 microscope features a fixed stage and focusing
nosepiece for maximum stability and accurate imaging.
An ultrasonic drive system enables fine, smooth
control of specimen position.
Effortless Imaging
The cellSens software allows researchers to
perform effortless imaging.
Accuracy
Ease of Use
Brain section of mouse at embryonic day 15 (Cy3)
5
Further Fluency in Fluorescence
Integration
The Olympus 8-position fluorescence
illuminator allows flexible responses to
various fluorescence specimens, with
easily replaceable mirror units.
Observations are accelerated
further with less need for
changing mirror units for
specimens such as multicolors and FISH.
NRK-52E cells (Alexa Fluor 488/Alexa Fluor 546)
6
BX63
Accuracy & Ease of Use
BX63
Intelligent Design for Maximum
Configuration Flexibility and Operating Ease
The BX63 offers outstanding stability and imaging precision for research
applications, with the convenience of a touch screen interface for easy
operation. Additionally, it features a detachable controller that can be
positioned to suit the operator's preference or workflow requirements.
Stability for Improved Imaging Reliability
Displacement
The Olympus BX63 is the choice for today’s motorized microscope.
By incorporating a motorized nosepiece and securely fastening the stage on three
points, the microscope is even more stabilized.
The smooth, high-precision drive of the fixed ultrasonic stage reduces vibrations,
further advancing the quality of image acquisition.
Large
Small
BX61
BX63
Stability Simulation in Comparison to BX61
Intuitive Touch Panel Controller for Enhanced Workspace and
Workflow Flexibility
Programmable controls greatly simplify repetitive observation and imaging tasks.
In Navigation mode, the interface is context-sensitive, and displays only the functions
that are relevant to the currently selected observation method.
In Full Operation mode, it allows researchers access to the full range of functions and
customization options available.
Touch Panel Controller
The Detachable Controller Customizes Workspace
into an Efficient Microscope Workstation
The controller U-MCZ can be removed and attached anywhere on the microscope,
enabling the researcher to create a working environment all his/her own.
The XY-controller of the ultrasonic stage can also be attached to the user’s preferred
side, allowing the simultaneous operation of the controller and the mouse.
The controller’s functionally-placed switches simplify observation method/objective/
mirror unit changeovers, light intensity adjustments and live/archive image selections.
U-MCZ
+BX3-SSU Ultrasonic Stage XY-controller
Configuration
Intelligent Microscope BX63 +
Digital Camera DP72
7
8
Motorized Units Facilitate Operations
Accuracy & Ease of Use
BX63
With the BX63, a diverse lineup of motorized units are available.
The researcher has a choice of three controls—the touch panel
controller, the controller/U-MCZ, or the imaging software
cellSens—for his/her observation method settings, microscopic
operations and digital imaging. Observations are made so much
faster and simpler, thanks to the interlocked motorized units
working together to satisfy even the most demanding applications.
Motorized Fluorescence Illuminator/
BX3-RFAA
The flexibility of the motorized fluorescence
illuminator accommodates multi-color
stained specimens.
The 8-position mirror units permit quick
changeover of fluorescence colors.
Motorized Attenuator Wheel/U-AW
The Olympus BX63 has a motorized ND
filter wheel for fluorescence and transmitted
light intensity adjustments.
Microscope Frame Equipped with
Motorized Focus and Field Diaphragm
This unit incorporates a high-speed, highprecision motorized focusing nosepiece with
0.1 µm resolution and 20 mm vertical
strokes. The field diaphragm adjustment of
transmitted light is also motorized.
Motorized Universal Condenser/
BX3-UCD8A
By integrating with designated optical
components, the motorized universal
condenser accommodates various kinds of
transmitted light observation, from brightfield
to differential interference contrast and
phase contrast.
* Special adapters are required for mounting (U-LHEAD
for fluorescence, and U-LH100ADP for transmitted light).
Motorized Seven Position Nosepiece/
U-D7REA
Equipped with a DIC slider slot, this
revolving nosepiece allows simultaneous
attachment of seven objectives. It is
especially suitable for continuous
observations from low to high magnifications
and combining specific objectives, such as
polarized light observations.
Scanning Stage with Ultrasonic/
BX3-SSU
The ultrasonic stage delivers high-precision
XY control. The XY-controller can be
mounted on the controller/U-MCZ for the
BX63 and worked like conventional stage
handles.
Software cellSens
Controller/U-MCZ
Touch Panel Controller
9
10
BX53
Accuracy & Ease of Use
BX53
A Great Solution for System Flexibility
with Comfortable Operability
The BX53 is a versatile system microscope that can be configured to
meet virtually any research need. It supports a wide range of fluorescence
imaging applications, and has a range of advanced features for enhanced
operating ease and process flexibility.
Saves Microscope Information with Coded Units
The imaging software cellSens integrated with the coded
fluorescence illuminator BX3-RFAS and the motorized 7-position
nosepiece U-D7RES can automatically store fluorescence mirror unit
and objective data with the images, facilitating post-imaging
treatments.
Customizable Control Layout
Light intensity now can be controlled
with the dial in front, and transmitted
filters and fluorescence shutters are
operable from either side. Detachable
fine focus handle can be attached on
either side of the microscope based
on operator preference. The BX53
frees the operator to create his/her
own working environment with the
microscope.
Objective Data
Further Ease in Imaging with
Multi-stained Specimens
The 8-position fluorescence
illuminator allows flexible responses
to various fluorescence specimens.
Mirror units can easily be replaced.
Mirror Unit Data Captured Image
Imaging Software
Automatic Switching of DIC Prisms
Switching objectives on the motorized 7-position revolving nosepiece,
integrated with the motorized universal condenser, enables an
automatic switch to the optimal DIC prism. Simplified prism switches
accelerate observations.
Changing Objectives with Coded
Nosepiece, Automatic Switchovers of
DIC Elements
Optimum Contrast Observation
Energy Saving Switch Turns off
Automatically
The motion sensor detects when an
operator leaves and will automatically
turn off the transmitted light lamp
after around 30 minutes. The energysaving switch conserves energy and
lamp lifetime.
System Microscope BX53
System Microscope BX53 +
Digital Camera DP72
11
12
UIS2 Objectives Deliver Optimal Performance
in Wider Wavelength Spectrum
UIS2 objectives
W.D.
(mm)
Cover
glass Immerthickness sion Spring
(mm)
Correction
ring
Iris Water proof
and
diaphragm oil proof
function
F.N.
Cover
glass Immerthickness sion Spring
(mm)
Objective
N.A.
UPLSAPO 4X
0.16
13
26.5
—
PLN 2X
0.06
5.8
22
UPLSAPO 10X2
0.4
3.1
26.5
0.17
PLN 4X
0.1
18.5
22
—
UPLSAPO 20X
0.75
0.6
26.5
0.17
PLN 10X
0.25
10.6
22
—
UPLSAPO 20XO
0.85
0.17
26.5
—
UPLSAPO 40X2
0.95
0.18
26.5
0.11-0.23
UPLSAPO 60XW
1.20
0.28
26.5
0.15-0.21
UPLSAPO 60XO
1.35
0.15
26.5
UPLSAPO 100XO
1.40
0.13
PLAPON 1.25X
0.04
PLAPON 2X
0.08
PLAPON 60XO
1.42
PLAPON 60XOSC
1.4
UPLFLN 4X
0.13
17
26.5
UPLFLN 10X2
0.3
10
26.5
F.N.
_
Oil
_
_
Objective
W.D.
(mm)
N.A.
—
PLN 20X
0.4
1.2
22
0.17
PLN 40X
0.65
0.6
22
0.17
_
_
Water
_
_
0.17
Oil
26.5
0.17
Oil
5
26.5
—
PLN 20XPH
0.4
1.2
22
0.17
6.2
26.5
—
PLN 40XPH
0.65
0.6
22
0.17
0.15
26.5
0.17
Oil
_
_
PLN 100XOPH
1.25
0.15
22
—
0.12
22
0.17
Oil
_
_
PLN 4XP
0.1
18.5
22
—
—
ACHN 10XP
0.25
6
22
—
—
ACHN 20XP
0.4
3
22
0.17
0.9-0.5
0.2
22
—
Oil
_
_
_
PLN 100XO
1.25
0.15
22
—
Oil
_
_
_
PLN 10XPH
0.25
10.6
22
—
0.5
2.1
26.5
0.17
_
ACHN 40XP
0.65
0.45
22
0.17
0.75
0.51
26.5
0.17
_
ACHN 100XOP 1.25
0.13
22
—
UPLFLN 40XO
1.3
0.2
26.5
0.17
MPLAPON100X
0.95
0.3
26.5
0
UPLFLN 60X
0.9
0.2
26.5
0.11-0.23
MPLAPON100XO
1.40
0.1
26.5
0
UPLFLN 60XOI 1.25-0.65 0.12
26.5
0.17
Oil
_
MPLFLN2.5X
0.08
10.7
26.5
—
0.2
26.5
0.17
Oil
_
MPLFLN10X
0.30
11
26.5
—
UPLFLN 100XOI2 1.3-0.6
0.2
26.5
0.17
Oil
_
MPLFLN20X
0.45
3.1
26.5
0
UPLFLN 10X2PH
10
26.5
—
MPLFLN40X
0.75
0.63
26.5
0
1.3
0.3
_
_
UPLFLN 20XPH
0.5
2.1
26.5
0.17
_
UPLFLN 40XPH
0.75
0.51
26.5
0.17
_
_
_
0
_
22
—
_
UAPON20XW340 0.70
0.35
22
0.17
Water
_
—
UAPON40XO340
1.35
0.1
22
0.17
Oil
_
UAPON40XW340 1.15
0.25
22
0.13-0.25
Water
_
Oil
_
_
UPLFLN 10XP
0.3
10
26.5
—
UPLFLN 20XP
0.5
2.1
26.5
0.17
UPLFLN 40XP
0.75
0.51
26.5
0.17
UPLFLN 100XOP
1.3
0.2
26.5
0.17
Oil
_
_
All UIS2 objectives and WHN eyepieces: lead-free eco-glass
_
UIS objective
_
_
Objective
N.A.
W.D.
(mm)
PLFL 100X
0.95
0.2
A wide range of observation tubes is available for the BX3 series,
including wide field binocular and trinocular types, various tilting
tubes, and tubes for observation of upright images in which the
specimen and the observed image move in the same direction.
Thanks to the application of
the original Olympus UW
multi-coatings, these Super
Apochromat objectives
compensate for both
spherical and chromatic
aberrations from the UV to the near infrared region. Their sensitivity to fluorescence
emissions ensures the acquisition of sharp, clear images, without color shift, even
in brightfield and Nomarski DIC observations. For quality and performance, they
offer solutions for digital imaging needs.
26.5
0.14-0.2
_
Correction
ring
Oil
Iris
proof
diaphragm cap
_
r
t
q
i
o
u
y
!3
!0
qU-TTBI/U-ETBI wU-TTLBI eU-TTR-2 rU-SWETR tU-SWETTR-5 yU-BI30-2 uU-TBI-3
!1
!2
iU-TR30-2/U-TR30NIR oU-SWTR-3 !0U-TBI-3-CLI !1U-ETR-4 !2U-EPA2 !3U-EPAL-2
Eyepieces
Eyepieces maintain image flatness even when a reflected light illuminator or other intermediate
tube is attached. The two available types are F.N. 22 and F.N. 26.5.
■ PLAPON Series
■ PLN(PLN-PH) Series
Designed for unsurpassed
resolution and contrast,
these Plan Apochromat
objectives keep chromatic
aberration down to a
minimum.
The PLAPON60XOSC objective has two improvements, chromatic aberration
compensation at 405 nm–650 nm and image-forming performance at 405 nm.
Ideal for a range of biological
applications, these high
quality objectives feature
excellent flatness up to F.N.
22 in transmitted brightfield
(phase contrast) observation.
The PLN-PH series is specifically designed for phase contrast work.
13
_
_
_
e
w
■ UPLFLN (UPLFLN-PH)
Series
These plan objectives also
provide flat images with high
transmission up to the near
infrared region of the
spectrum. With their high
S/N ratio, excellent resolution and high contrast imaging, they are especially
effective in brightfield and Nomarski DIC observations. The UPLFLN-PH series is
optimized for phase contrast observation.
_
Cover
glass ImmerF.N. thickness sion Spring
(mm)
Observation Tubes / Eyepoint Adjusters
■ UPLSAPO Series
_
26.5
Oil
_
Oil
1
0.17
26.5
_
_
20
0.17
17
_
_
_
Oil
0.90
26.5
0.13
_
_
_
0.10
26.5
UPLFLN 4XP
_
_
_
Oil
MPLN5X
UPLFLN 100XO2PH
0.2
_
_
_
MPLFLN100X
UPLFLN 60XOIPH 1.25-0.65 0.12
1.3
_
PLN 50XOI
UPLFLN 40X
UPLFLN 100XO2
_
_
UPLFLN 20X
Oil
Iris Water proof
and
diaphragm oil proof
function
Correction
ring
Eyepiece specifications
Item
Widefield
Name
Diopter
Micrometer (ømm)
22
-8 — +5
24
22
-8 — +5
22
WHN10X-H
CROSSWHN10X
Super widefield
F.N.
WHN10X
24
SWH10x-H
26.5
-8 — +2
MICROSWH10X
26.5
-8 — +2
CROSSSWH10X
26.5
-8 — +2
—
14
World-renowned Optical Performance Accommodates
Various Observation Styles
Fluorescence
Nomarski DIC
Olympus Takes Fluorescence Observation to Another Plane
Image Optimization According to Specimen Characteristics
Brain section of mouse at embryonic day 15 (GFP)
Tracheal primordia of Drosophila embryo (GFP)
Distal Tip of a Drosophila limb (GFP)
Brain section of mouse at embryonic day 15
Gastrulation stage chicken embryo (DAPI/Alexa Fluor 488/Alexa Fluor 594)
NRK-52E cells (Alexa Fluor 488/Alexa Fluor 546)
Blood island at stage 12 of chicken development
A shoot apical meristem of rice
A total of three types of reflected illuminators are available, the
motorized fluorescence illuminator BX3-RFAA, the coded
fluorescence illuminator BX3-RFAS, and the universal reflected
illuminator BX3-URA.
Eight fluorescence mirror units can be attached for comfortable
multi-color fluorescence observations. Fly’s-eye lenses, that are
equipped with both BX3-RFAA and BX3-RFAS, and highperformance filters that come with mirror units realize bright, efficient
fluorescence observations. Storing fluorescence mirror unit data
(used in imaging) together with the images is possible by integrating
coded fluorescence illuminator and motorized fluorescence
Illuminator.
BX3-URA
BX3-UCD8A
U-UCD8-2
Olympus has prepared a wider selection of DIC sliders with varied
shearing value for acquiring optimal specimen images. The U-DICT
and the U-DICTS are designed for all-round performance. The
U-DICTHC optimizes high-contrast observations of thin specimens,
and the U-DICTHR, high resolution with less glare for thick
specimens.
Two types of condensers are also available: the 8-position universal
condenser U-UCD8-2 and the
motorized universal condenser
BX3-UCD8A, both for various
observations (brightfield,
darkfield, phase contrast, DIC
and simple polarized light).
BX3-RFAS
q
BX3-RFAA
U-CO1.25x
U-TLD
U-TLO
w
e
r
qU-DICT wU-DICTS eU-DICTHR
rU-DICTHC
15
16
Brightfield
Polarized Light
Brighter Images, with Superb Resolution/flatness
at All Magnifications
Polarizing Observation for Wide-area Retardation
Measurement
A diverse condenser lineup includes the achromatic aplanatic
U-AAC, delivering excellent resolution and flatness from low to high
magnifications, the swing-out U-SC3 accommodating 1.25x to 100x,
the low magnification U-LC for
w
r
continuous 2x to 100x (Dry)
observations, and the ultra low
magnification U-ULC-2.
Cerebrum (Bodian Stain)
Tooth, bone, muscle tissue, nerve tissue, actomyosin fiber and mitotic
spindle can all be observed, without staining. There are intermediate
attachments (U-OPA/U-CPA) for orthoscopic and
orthoscopic/conoscopic viewing.
w
Various compensators make it
t
possible to observe a wide range of
retardation. Also available is a
q
condenser exclusively for polarization
observation, revolving nosepiece,
r
e
rotating stage, objectives and simple
polarizing attachment.
qU-POC-2 wU-CPA eU-OPA
Pancreas (HE Stain)
Asbestos
q
e
t
qU-SC3 wU-ULC-2 eU-AC2 rU-AAC tU-LC
Liver (Azan Stain)
rU-AN360P-2 tU-P4RE
Urate Crystals
Phase Contrast
Darkfield
Ideal Phase Contrast Observation with Excellent
Image Clarity
High-quality Darkfield Effect at All Magnifications
High contrast phase imaging allows close observation of the cell
interior and of live bacteria. Using UPLFLN-PH or PLN-PH series
objectives, phase contrast observation from 10x up to 100x is
achievable. With the U-PCD2
phase/darkfield condenser, users can
view specimens in brightfield or
darkfield. Simultaneous observation
with reflected light fluorescence
microscopy is also possible.
Two darkfield condensers are provided: the dry darkfield condenser
(U-DCD), for magnifications from 10x to 100x (up to N.A. 0.80); and
the oil immersion darkfield condenser (U-DCW), for magnifications
from 20x to 100x (up to N.A. 1.2).
* Please consult your nearest Olympus
representative for applicable objectives.
U-DCD
U-PCD2
NRK-52E cells
NRK-52E cells
17
Amyloid
NRK-52E cells
U-DCW
Spirogyra
Water Flea
18
Microscope Digital Cameras to Fulfill Diverse Needs
Imaging Software / cellSens
DP72
High-speed, High-definition Image Capture Provides Smooth Fluorescence Imaging
Thanks to its high-speed hardware, the DP72 can capture highresolution images equivalent to 12.8 million pixels* in around 2.5
seconds. The camera's high sensitivity and low noise (equivalent
to the level of ISO 1600) ensure clear fluorescence imaging, while
the resolution quality allows precise representation of particular
specimen areas.
*By shifting the pixels of the 1.45 million pixel 2/3 inch CCD (one pixel = 6.45 um), it is possible to
record still images equivalent to the maximum image recording size (4140 x 3096) or effective image
size of 12.8 million pixels.
cellSens functions
cellSens is an imaging software available in three
versions to meet individual workflow needs.
"Entry" is used for simple image acquisition. “Standard”
provides simple operation for imaging documentation.
“Dimension” allows for the control of the complete
workflow from image capture to analysis. With GUI
customization, working environments for both Standard
and Dimension can be optimized according to
workflow. cellSens frees the researcher to concentrate
on his/her creative work.
DP25
High-definition, High-resolution 5-million Pixel Technology
The DP25 provides live display at a high frame rate of 8 fps with
exceptional quality of 2560 x 1920 pixels. In addition, the DP25 is
equipped with a color profile that provides full-color images in realtime, allowing true color reproduction of specimens. It can easily
be connected with just one cable (6-pin) to a PC with a FireWire
(IEEE1394) port. It can also connect to a laptop PC via a FireWire
(IEEE1394a) PC card.
Layout
View
User experience customization
Tile view (multiple images in a single data set shown side by side)
Image acquisition Snap/Movie acquisition
Multi-dimensional (xyzt and wavelength)
Instantly create EFI image (manual or motorized Z)
Image analysis
*Available from version 1.4.
Fluorescence unmixing
3D deconvolution (constrained iterative deconvolution)
Region and line measurements
Object analysis and classification
Documentation
Automatically compose Word reports
Remote viewing
NetCam (live image data transmission over the internet)
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Count & Measure
DP21
The Optimal Stand-alone Model for Conferences
The DP21 is a stand-alone digital camera with a convenient
handset for simple operations from observations to imaging. Its
accurate color reproductions and smooth, high-definition live
image displays are ideal for small discussion groups and
conferences. Optional cellSens imaging software platform also
allows operation via computers.
Camera Adapters
The single port tube of the
trinocular tube is detachable,
and can be used with various
cameras through a range of
adapters.
Camera Adapter
(projection lens)
U-TV1xC
Projection Area (F.N.)
Projection
Magnifications 2/3" CCD 1/2" CCD 1/3" CCD
1x
11
8
6
U-TV1x-2+U-CMAD3
1x
11
8
6
0.63x
17.5
12.7
9.5
U-TV0.5xC-3
0.5x
22
16
12
U-TV0.35xC
0.35x
22
16
12
U-TV0.63xC
Practical Field of View (mm) =
19
Projection Area (field number)
Objective Magnifications
U-TV0.63×C
U-TV1×C
U-TV0.35×C-2
U-TV1×-2+
U-CMAD3
U-TV0.5×C-3
20
BX63 system diagram
TR-Adapter
CAMERAS
U-FMT
F mount adapter
U-SMAD
Sony
mount adapter
U-TMAD
T mount adapter
WHN10X,
WHN10X-H,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-TVZ
Zoom camera
port
U-BMAD
Bayonet
mount adapter
U-CMAD3
C mount adapter
U-KPA
Intermediate attachment for
simple polarizing observation
U-CMDPTS
C mount adapter
for U-DPTS
U-TV1x-2
Direct image
camera port
U-DPCAD
Dual port tube
with C mounts
U-ANT
Analyzer for
transmitted light
U-DPTS
Multi double port tube
U-TV0.25xC
C mount
camera port
with 0.25x lens
U-CMDPTS
C mount adapter
for U-DPTS
U-TV0.35xC-2
C mount
camera port
with 0.35x lens
U-TV0.5xC-3
C mount
camera port
with 0.5x lens
U-TV0.63xC
C mount
camera port
with 0.63x lens
U-TV1XC
C mount camera
adapter
U-CA
Magnification changer
U-EPA2
Eyepoint adjuster
U-TR30NIR
Trinocular tube
U-ETR-4*1
Erect image
trinocular tube
U-TTR-2
Tilting trinocular
tube
U-TR30-2
Trinocular tube
U-BI30-2
Binocular tube
Tilting binocular tube
A
U-EPAL-2
Eyepoint adjuster
U-TTLBI*2
Tilting, telescopic,
lifting binocular tube
U-LH75XEAPO
75 W xenon apo
lamp housing
A
U-IFCBL50
Interface cable, 50 cm
U-ANT
Analyzer for
transmitted light
U-ETBI
Ergonomic
erect image
binocular tube
U-TTBI
Ergonomic
binocular tube
E
U-D7REA
Motorized 7-position nosepiece
U-GAN
Gout analyzer
U-TBI-3-CLI*1
Tilting binocular
tube
U-TBI-3*1
U-RFL-T
BX3-RFAS
Coded fluorescence illuminator
U-D7RES
Coded 7-position
nosepiece
U-RX-T
Power supply unit
for xenon lamp
A
U-DICT
DIC slider for
transmitted light
U-LH100HGAPO
100 W mercury apo
lamp housing
E
LIFE TIME
BURNER ON
OBJECTIVES
U-RFL-T
U-DICTS
Shift DIC slider for transmitted light
U-DICTHR
High resolution DIC slider
for transmitted light
U-DICTHC
High contrast DIC slider
for transmitted light
U-LHEAD
Extension adapter
for lamp housing
BX3-ARM
Standard arm
Mirror units
E
U-LH100HG
100 W mercury
lamp housing
U-DULHA
Double lamp housing
adapter
U-AN-2
Analyzer slider
U-RFL-T
Power supply unit
for mercury lamp
A
U-LH100ADP
LH100 adapter
U-AW
Motorized
attenuator wheel
U-IFCBL15
Interface cable, 15 cm
U-IFCBL200
Interface cable, 200 cm
A
E BX3-RFAA
B
U-IFCBL200
Interface cable,
200 cm
Motorized
fluorescence
illuminator
C
U-MCZ
Controller
BX63F
BX63 frame
U-POT
Polarizer
U-AW
Motorized
attenuator wheel
B
U-LH100-3
100 W halogen
lamp housing
U-LHLEDC
LED lamp
housing
U-RMT
Extension cord
A
U-IFCBL15
Interface cable, 15 cm
U-IFCBL200
Interface cable, 200 cm
A
B
C1394A
Interface cable
B
C
D
U-IFCBL15
Interface cable, 15 cm
BX3-CBH
Control box
Filter (ø45)
Touch panel controller
(attached to BX63F)
U-FC
Filter cassette
LIFE TIME
BURNER ON
PC (software)
U-TLO
Oil top lens
U-TLD
Dry top lens
U-HLD-4,
U-HLDT-4
Specimen holder
U-HRD-4,
U-HRDT-4
Specimen holder
IX2-GCP
Glass stage
insert plate
U-HLS-4,
U-HLST-4
Specimen holder
IX-CP50
Insert plate
IX-SCL
Slide clip
XY-controller
(attached to BX3-SSU)
U-CST
Centering target
U-SC3
Swing-out
condenser
U-AAC
Achromatic/
Aplanatic
condenser
U-ULC-2
Ultra low
condenser
U-DCD
Darkfield
condenser, dry
U-DCW
Darkfield
condenser, oil
Optical elements
D
U-SHG
U-SHGT
Rubber grips
U-SVLO
Oil rectangular
stage with
left-hand
control
U-SVRO
Oil rectangular
stage with
right-hand
control
U-SVLB-4
Mechanical
stage with
left-hand
control
U-SVRB-4
Mechanical
stage with
right-hand
control
Control box
(attached to BX3-SSU)
BX3-SSU
Scanning stage
with ultrasonic
A
BX3-UCD8A
Motorized universal
condenser
IX-SVL2*3
Cross stage with short
left handle
U-IFCBL15
Interface cable, 15 cm
U-TLO
Oil top lens
U-TLD
Dry top lens
U-CO1.25x
Low magnification
conversion lens for UCD
U-UCD8-2
8-position universal condenser
U-UCDTP530
Tint plate for
sensitive color
observation
BX3-STAD
Stage adapter
*1 Slight vignetting may occur in combination with an additional intermediate attachment or fluorescence illuminator. *2 Require an additional intermediate attachment or fluorescence illuminator.
21
*3 Cannot be used with BX3-UCD8A.
22
BX53 system diagram
TR-Adapter
U-ECA
Magnification changer 2x
CAMERAS
U-FMT
F mount adapter
U-TMAD
T mount adapter
U-ECA1.6x
Magnification changer 1.6x
U-CMAD3
C mount
adapter
U-BMAD
Bayonet
mount
adapter
U-SMAD
Sony
mount
adapter
U-ANT
Analyzer for
transmitted light
U-KPA
Intermediate attachment for
simple polarizing observation
WHN10X-H,
* WHN10X,
CROSS WHN10X
Eyepieces
U-CT30-2
Centering telescope
U-TVZ
Zoom camera
port
▲ SWH10X-H,
U-TV1x-2
Direct image
camera port
CROSS SWH10X,
MICRO SWH10X
Eyepieces
U-CT30-2
Centering telescope
U-SWETTR-5
Super widefield
erect image
tilting trinocular
observation tube
U-CA
Magnification changer
U-CMDPTS
C mount adapter
for U-DPTS
U-DPCAD
Dual port tube
with C mounts
U-TV0.35xC-2
C mount
camera port
with 0.35x lens
U-TV0.25xC
C mount
camera port with
0.25x lens
U-CMDPTS
U-DPTS
Multi double port tube C mount adapter
for U-DPTS
U-TV0.5xC-3
C mount
camera port
with 0.5x lens
U-TV0.63xC-3
C mount
camera port
with 0.63x lens
U-TV1xC
C mount
camera
adapter
U-DP1xC
Dual port 1x
U-DP*1*3
Dual port
U-APT
Arrow pointer
U-SWETR
Super widefield
erect image
trinocular
observation tube
U-ETR-4*1
Erect image
trinocular tube
U-SWTR-3
Super widefield
trinocular tube
*
U-TR30NIR
Trinocular tube
U-TR30-2
Trinocular tube
*
U-TTR-2
Tilting trinocular tube
*
*
U-TBI-3*1
Tilting binocular tube
U-BI30-2
Binocular tube
*
*
U-TBI-3-CLI*1
Tilting binocular tube
*
*
U-EPA2
Eyepoint adjuster
*
*
U-EPAL-2
Eyepoint adjuster
U-TTBI
Ergonomic
binocular tube
U-TTLBI*2
Tilting,
telescopic,
lifting binocular
tube
U-ETBI
Ergonomic erect
image binocular
tube
U-TRU*1*3
Trinocular intermediate unit
U-TRUS*1
C
U-GAN
Gout analyzer
U-ANT
Analyzer for
transmitted light
A
B
U-D7REA
Motorized
7-position
nosepiece
U-D7RES
Coded 7-position
nosepiece
U-D6RE
Sextuple revolving
nosepiece for
DIC/simple POL
U-D7RE
Septuple revolving
nosepiece for
DIC/simple POL
U-P4RE
Centerable
revolving
nosepiece
U-CPA
Intermediate attachment for
conoscopic andorthoscopic
observation
U-5RE-2
Quintuple
revolving
nosepiece
U-AN360P-2
Rotatable analyzer
U-OPA
Intermediate attachment for
orthoscopic observation
U-DICT
DIC slider for
transmitted light
OBJECTIVES
BX3-ARM
Standard arm
U-DICTS
Shift DIC slider for
transmitted light
U-DICTHR
High resolution DIC
slider for transmitted light
U-DICTHC
High contrast DIC slider
for transmitted light
C
BX53F
BX53 frame
A
Motorized fluorescence
illuminator
U-AN-2
Analyzer slider
Mirror units
U-RFL-T
U-LH75XEAPO
75 W xenon
apo lamp
housing
BX3-RFAA*4
C
U-LH100HGAPO
100 W mercury
apo lamp
housing
BX3-RFAS*4
Coded fluorescence
illuminator
C
U-POT
Polarizer
U-LHEAD*5
Extension
adapter for
lamp housing
B
U-RX-T
Power supply unit
for xenon lamp
U-TAD
Plate adapter
U-SDO3
Side by side observation
attachment
LIFE TIME
U-LH100HG
100 W mercury
lamp housing
U-RFL-T
Power supply unit
for mercury lamp
A
U-FC
Filter cassette
C
U-AN-2
Analyzer slider
COMPENSATORS
U-HLD-4,
U-HLDT-4
Specimen
holder
U-CST
Centering
target
U-FMP
Mechanical
stage
U-SRP
Precision
rotatable
stage
BX3-25ND6
ND filter
BX3-25ND25
ND filter
U-AW
Motorized attenuator wheel
U-MDO10B3
Multi observation body
for 10 persons
U-MDO10R3*6
Multi observation body for 10 persons
U-HRD-4,
U-HRDT-4
Specimen
holder
U-HLS-4,
U-HLST-4
Specimen
holder
U-SHG
Rubber grip
U-SHGT
Rubber grip
U-SP
Plain
stage
U-MDOSV*6
Multi observation
side viewer
U-MDOB3
Multi observation
body
BX3-URA
Universal reflected illuminator
Filter (ø45)
U-DO3
Dual observation attachment
BURNER ON
U-DULHA
Double
lamp house
adapter
U-AN-2
Analyzer slider
U-LH100-3
100 W halogen
lamp housing
U-DAL10X
Drawing attachment 10X
U-DA
Drawing attachment
LIFE TIME
BURNER ON
U-RFL-T
U-ANT
Analyzer for
transmitted light
U-SRG2
Rotatable
graduated
stage
U-P6RE
Centerable sextuple
revolving nosepiece
Trinocular intermediate unit
U-AC2
U-LC*7
Abbe
Low
magnification condenser
condenser
U-SC3
Swing-out
condenser
U-AAC
U-ULC-2
Achromatic/ Ultra low
Aplanatic
condenser
condenser
U-PCD2
U-POC-2
Phase/darkfield Polarizing
condenser
condenser
U-HSEXP
Hand switch for
exposure
Stand*6
U-CBS
Control box for
coded function
U-SVRO
Oil rectangular
stage with
right-hand
control
U-SVLO
Oil rectangular
stage with
left-hand
control
U-SVRB-4
Mechanical
stages with
right-hand
control
U-SVLB-4
Mechanical
stages with
left-hand
control
Optical devices
U-CO1.25x
Low magnification
conversion
lens for UCD
A
BX3-UCD8A
Motorized universal
condenser
U-TLO
Oil top lens
U-TLD
Dry top lens
U-HSCBM
Hand switch for CBM
U-DCD
Darkfield
condenser,
dry
U-DCW
Darkfield
condenser,
oil
B
DP21-CB
Control box
A
*1 Slight vignetting may occur in combination with an additional intermediate attachment or observation method. *2 Require an additional intermediate attachment or fluorescence illuminator.
*3 Cannot be used with U-TTLBI. *4 Compatible with F.N. 22. *5 Cannot be used with BX3-URA. *6 Stand is a standard equipment of the U-MDOSV and U-MDO10R3. *7 An auxiliary lens is equipped.
23
U-UCDTP530
Tint plate for
sensitive color
observation
U-UCD8-2
8-position
universal condenser
U-IFCBL200
Interface cable,
200 cm
U-CBM
Control box
PC (Software)
24
UIS2 optical system
Focus
Built-in motorized nosepiece focus
Stroke: 20 mm, minimum increment: 0.01 µm, maximum nosepiece movement speed: 3 mm/s
Illuminator
Built-in Koehler illumination for transmitted light, Light intensity LED indicator, Built-in motorized field stop
• High color reproductivity LED light source •12 V 100 W halogen bulb (pre-centered)
Widefield (F.N. 22)
• Widefield tilting trinocular • Widefield trinocular • Widefield erect image trinocular
• Widefield tilting binocular • Widefield tilting,Telescopic,Lifting binocular tube • Widefield ergo binocular
• Widefield binocular
(unit: mm)
220
Touch panel controller dimensions
(unit: mm)
212.5*
• Motorized septuple revolving nosepiece • Interchangeable reversed coded septuple nosepiece
ECO
mode
• Motorized 6-position ND filter wheel
Fluorescence illuminator
• Motorized multi-purpose coded type (F.N. 22, motorized 8-position mirror unit turret, 4-position ND slider)
• Multi-purpose coded type (F.N. 22, 8-position mirror unit turret, 4-position ND slider)
Fluorescence light source
• 100 W Hg apo lamp housing and transformer
• 100 W Hg lamp housing and transformer
• 75 W Xe lamp housing and transformer
Controller
• High-performance control box ( I/F: FireWire)
MAX
ECO
mode
FL SHUTTER
OPEN CLOSE
OPEN CLOSE
DF
FL PH DIC BF PO DF
190
OBSERVATION
210.5
FL MIRROR UNIT
UNIT
• Motorized universal condenser (N.A. 0.9, motorized 8-position turret, Aperture stop, polarizing filter in/out mechanism
and top lens swing out mechanism), for 1.25x–100x [swing-out 1.25x-4x, with oil top lens: (N.A. 1.4)]
• Swing out Achromatic (N.A. 0.9), for 1.25x–100x (swing-out: 1.25x–4x)
• Achromatic Aplanatic (N.A. 1.4), for 10x–100x
• Universal (N.A. 0.9), for 1.25x–100x [swing-out: 1.25x–4x, with oil top lens:(N.A. 1.4)]
• Ultra low (N.A. 0.16), for 1.25x–4x
• Darkfield dry (N.A. 0.8–0.92), for 10x–100x
• Darkfield oil (N.A. 1.20–1.40), for 10x–100x
ND filter wheel
OFF
MAX
FL SHUTTER
3
8
1
2
3
4
5
6
7
8
OLYMPUS
TURRET
POL.
TOP LENS
AS
209
Weight: Approx. 2.4 kg, Power consumption: Approx. 25 W
96.2
Condenser
104.5
164
OFF
25––
• Ultrasonic stage (Stage stroke: X:76 mm x Y: 52 mm,
maximum stage movement speed: 30 mm/s
• Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque
adjustment mechanism, optional rubber grips available
• Cross stage with short left handle
294.5
152.5
BX3-CBH dimensions
429
(unit: mm)
656
680.5
Weight: Approx. 35 kg, Power consumption: Approx. 440 W
The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.
BX53 FL dimensions
(unit: mm)
214
*This device is designed for use in industrial environments for the EMC performance (EC61326-1 Class A device). Using it in a residential environment may affect other equipment in the environment.
175
210
Stage
AS
FS
582.5*
Revolving nosepiece
Observation tube
BX63 FL dimensions
Optical system
45
Microscope frame
160
BX63 specifications
184*
BX53 specifications
UIS2 optical system
Focus
Vertical stage movement: 25 mm stage stroke with coarse adjustment limit stopper, Torque adjustment for coarse adjustment
knobs, Stage mounting position variable, High sensitivity fine focusing knob (minimum adjustment gradations: 1 µm)
124
Weight: Approx. 4.2 kg, Power consumption: Approx. 230 W
Interchangeable reversed quintuple/coded quintuple/sextuple/septuple/coded septuple nosepiece
Super widefield (F.N. 26.5)
• Widefield tilting, telescopic and lifting binocular
• Widefield tilting trinocular • Widefield trinocular • Widefield erect image trinocular • Widefield tilting binocular
• Widefield tilting,Telescopic,Lifting binocular tube •Widefield ergo binocular •Widefield binocular
• Super widefield trinocular • Super widefield erect image trinocular
Stage
Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque adjustment
mechanism, optional rubber grips available (Non stick grooved coaxial, plain, rotatable stages are also available)
Condenser
• Abbe (N.A. 1.1), for 4x–100x
• Swing out Achromatic (N.A. 0.9), for 1.25x–100x (swing-out: 1.25x–4x)
• Achromatic Aplanatic (N.A. 1.4), for 10x–100x
• Phase contrast, darkfield (N.A. 1.1), [phase contrast: for 10x–100x, darkfield: for 10x–100x (up to N.A. 0.80)]
• Universal (N.A. 0.9), for 1.25x–100x [swing-out: 1.25x–4x, with oil top lens:(N.A. 1.4)]
• Low (N.A. 0.75), for 2x–100x(Dry)
• Ultra low (N.A. 0.16), for 1.25x–4x
• Darkfield dry (N.A. 0.8–0.92), for 10x–100x
• Darkfield oil (N.A. 1.20–1.40), for 10x–100x
Fluorescence illuminator
• Multi-purpose coded type (F.N. 22, 8-position mirror unit turret, 4-position ND slider)
• Economical type (F.N. 26.5, 8-position mirror unit turret)
Fluorescence light source
• 100 W Hg apo lamp housing and transformer
• 100 W Hg lamp housing and transformer
• 75 W Xe lamp housing and transformer
*The U-CBM is designed for the BX3 use in industrial environments for the EMC performance (IEC61326-1 Class A device). Using it in a residential environment may affect other equipment in the environment.
81.5
Widefield (F.N. 22)
SHUTTER
209
Observation tube
AS
FS
Built-in Koehler illumination for transmitted light, Light preset switch, Light intensity LED indicator, Built-in filters (LBD-IF, ND6,
ND25, optional), 12 V 100 W halogen bulb (pre-centered)
469*
Revolving nosepiece
280
294.5
440.5
Illuminator
100
45
Optical system
447
Microscope frame
90
91.5
274.5
362
436.5
521.5
Weight: Approx 21 kg, Power consumption: Approx. 290 W
The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.
BX53 BF dimensions
(unit: mm)
184*
175
BX63/BX53 common specifications
45
410*
65
• Indoor use
• Ambient temperature
: 5 º to 40 ºC (41 º to 104 ºF)
• Maximum relative humidity : 80 % for temperatures up to 31º C (88 ºF), decreasing linearly through 70 % at 34 ºC
(93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)
• Supply voltage fluctuations : Not to exceed ±10 % of the normal voltage
81.5
209
Operating environment
90
274.5
91.5
362
436.5
Weight: Approx. 16 kg, Power consumption: Approx. 140 W
The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.
25
26
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