Fluorescence Filter Cubes

ECLIPSE 5Oi/55i Accessories
Accessories
Clinical & Laboratory Microscope Accessories
/
ECLIPSE i-Series Website
www.nikon-i.com
Cytodiagnosis Tests
Cytodiagnostic Accessories
This compact Ergo-View cytodiagnostic unit can be easily
retrofitted to the 50i or 55i microscope, to facilitate easier and
more comfortable cytology examinations.
•Motorized magnification switchover between 10X and 40X at the flip of a switch.
•Rapid and quiet magnification changeover with no vibration.
This eliminates eye strain due to image blur.
•Excellent image parfocality minimizes focus deviation, eliminating the need for
focus adjustment each time the magnification is changed.
•In configuration with the ECLIPSE 55i that adopts LED illumination, light intensity
is automatically adjusted following the magnification change. This ensures
comfortable observations with constant brightness.
•Easy object marking while observing the specimen through eyepieces.
•Marking ink is a cartridge type and can be easily exchanged.
■ Specific Accessories for ECLIPSE 50i
1 J-CY Cytodiagnostic Unit
2
2 DS AC Adapter
3 C-HS Hand Switch
1
3
■ Specific Accessories for ECLIPSE 55i
1 J-CY Cytodiagnostic Unit
1
2 Cable Set for Cytodiagnostic Unit
2
3 C-HS Hand Switch
3
Phase Contrast
Phase Contrast Accessories
(ECLIPSE 50i)
These accessories produce well-defined high contrast images
with neutral background coloration regardless of the
magnification range. The CFI Plan Fluor DLL objectives can be
universally used for phase contrast, epi-fluorescence,
brightfield, as well as DIC applications. The CFI Achromat ADL
objectives clearly visualize the minute structure of thick phase
objects by minimizing halos.
■ Specific Accessories
1
3
1 C-C Phase Contrast Condenser
2 CFI Plan Fluor DLL Objectives
3 Filter, 45mm GIF
4
2
2
4 C-CT Centering Telescope
Darkfield
Darkfield Accessories (ECLIPSE 50i)
These darkfield accessories allow clear observation of blood
or the minute structure of flagella. Dry- and oil-type
condensers are available. The expander lens is used to obtain
illumination with greater brightness. It is also possible to
perform darkfield microscopy using a phase contrast
condenser.
■ Specific Accessories
1 C-CEL Expander Lens
2 C-C Darkfield Condenser (Dry)
3 C-C Darkfield Condenser (Oil)
1
2
3
Simple Polarizing
Simple Polarizing Accessories ( ECLIPSE 50i )
Ideal for observing birefringent samples such as collagen,
amyloids and crystals. Operation is as simple as inserting a
polarizer over the field lens and an analyzer in the arm slot.
The field number has been increased to 25.
■ Specific Accessories
1 C-SP Simple Polarizer
1
2 C-ISA Intermediate Tube w/Simple Analyzer
2
Sensitive Color Polarizing Epi-fluorescence
Sensitive Color Polarizing Accessories ( ECLIPSE 50i )
Enables easy gout and pseudo-gout tests.
■ Specific Accessories
1
1 C-TP Polarizer with First-order Red Tint Plate
2 C-IA Intermediate Tube w/Analyzer
2
Epi-fluorescence Accessories
The epi-fl illuminator is a compact and easy-to-use turret-type
exclusively developed for the ECLIPSE 50i and 55i.
■ Specific Accessories
•Four filter cubes can be accommodated, and they are easily exchanged.
•Filters or mirror in the filter cubes can be easily replaced to create the desired
combination.
•Filter position labels glow in the dark for easy identification.
1 J-FL Epi-fluorescence Attachment
2 Filter Position Labels
4
3 Light Shielding Plate
4 Hg Lamphouse HMX-4B
1
5 C-FC Epi-fl Collector Lens
6 Light Shielding Tube
6
5
2
7 C-FC Centering Tool
8
8 C-SHG1 Power Supply for HG 100W
7
9 C-FL Epi-fl Filter Cubes
9
! CFI Plan Fluor Objectives
3
!
3
Epi-fluorescence Filters
Fluorescence Filter Cubes
Filters
UV-2A
•Standard filter block for UV
•For DAPI, cutting off FITC (green) and TRITC (red)
•Soft-coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off green and red
UV-1A
EX 365/10
DM 400
BA 400
•Narrow band pass – only 365nm (i line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
UV-2B
EX 330-380
DM 400
BA 435
•Darker background than UV-2A
V-2A
EX 380-420
DM 430
BA 450
•Standard filter block for V
BV-2A
EX 400-440
DM 455
BA 470
•Standard filter block for BV
BV-1A
EX 435/10
EM 455
BA 470
•Narrow band pass – only 435nm (g line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
B-3A
EX 420-490
DM 505
BA 520
•Wide band pass – recommended for halogen illumination only
B-2A
EX 450-490
DM 505
BA 520
•Standard filter block for B
•For FITC + Counter-stain (TRITC, PI)
B-2E/C
(FITC)
EX 465-495
DM 505
BA 515-555
•Soft coated type for high signal/noise
•For FITC (green), cutting off Rhodamine red
•Band-pass Barrier Filter used to cut off red
B-1A
EX 470-490
DM 505
BA 520
•Narrower excitation range than B-2A
•FITC+Counter-stain (TRITC, PI)
B-1E
EX 470-490
DM 505
BA 520-560
•For FITC (green), cutting off Rhodamine red
•Band-Pass Barrier Filter used to cut off red
G-2A
EX 510-560
DM 575
BA 590
•Standard filter block for G
G-2E/C
(TRITC)
EX 540/25
DM 565
BA 605/55
•For TRITC (Rhodamine)
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 643nm
G-1B
EX 546/10
DM 575
BA 590
•Narrow band pass – only 546nm (e line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
G-2B
EX 510-560
DM 575
BA 610
•610nm barrier provides darker background and deep red emission
Y-2E/C
(Texas Red)
EX 540-580
DM 595
BA 600-660
•For Texas Red®
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 660nm
G
Y
Fluorescence Reagents and Dedicated Filters*1
for Fluorescence Protein
Wavelengths
Filters/Reagents
GFP-L
GFP-B
BFP
CFP
YFP
Cy 3
Cy 5
Cy 7
4
Applications
EX 340-380
DM 400
BA 435-485
BV
B
Characteristics
UV-2E/C
(DAPI)
UV
V
Wavelengths
EX 330-380
DM 400
BA 420
EX480/40, DM505, BA510
EX480/40, DM505, BA535/50
EX380/30, DM420, BA460/50
EX436/20, DM455, BA480/40
EX500/20, DM515, BA520
EX535/50, DM565, BA610/75
EX620/60, DM660, BA700/75
EX710/75, DM750, BA810/90
•DAPI
•Hoechst 33258/33342
•AMCA
•Cascade Blue®
•Autofluorescence
•Catecholamine
•Serotonin
•Tetracycline
•Quinacrine
•Quinacrine Mustard (QM)
•Thioflavine S
•Acriflavine
•FITC
•Acridine Orange
•Auramine O
•Coriphosphine O
•Bodipy®
•Fluo-3
•DIO
•TRITC
•Rhodamine B200
•Propidium iodide
•R-Phycoerythrin
•B-Phycoerythrin
•Dil
•Ethidium Bromide
•Texas Red®
Multi-Band Filters*1
Filters
Abbreviations Reagents
F-R
FITC/Rhodamine
Dual
Triple
F-T
FITC/Texas Red
D-F
DAPI/FITC
D-F-R
DAPI/FITC/Rhodamine
D-F-T
DAPI/FITC/Texas Red
Filters are consumables. Filters marked with an asterisk (*1), in particular, come with special coatings,
so these should be replaced in the shortest possible period of time.
Other filter combinations are available. Please ask your local Nikon distributor for a complete list of filters
or inquire about special custom filter combinations possible.
CFI Objectives
CFI Objectives
Brightfield
(CFI)
0.10
30.00
-
4×
0.13
17.10
-
0.25
7.00
-
10×
0.30
16.00
0.17
LWD 20×
0.40
3.90
0.17
20×
0.50
2.10
0.17
40×
0.65
0.65
0.17
0.75
0.35
0.17
LWD 40×C
0.55
2.7-1.7
0-2.0
20×Mi
(Multi-immersion)
60×
0.80
0.30
0.17
40×
0.75
0.72
0.17
1.30
0.20
0.17
0.85
0.30
0.11-0.23
0.5-1.25
0.22
0.17
0.90
0.30
0.14-0.20
1.25
0.23
0.23
0.17
0.25
7.00
-
Achromat
0.40
3.90
0.17
0.40
3.10
1.2
LWD DL 40×C
0.65
0.55
1.25
0.25
0.65
2.7-1.7
0.23
6.10
1.2
3.10
1.2
LWD ADL 40×C
0.55
2.7-1.7
0-2.0
P 4×
0.10
30.00
-
0.25
7.00
-
LWD P 20×
0.40
3.90
0.17
P 40×
0.65
0.65
0.17
P 100×H
1.25
0.23
0.17
0.04
3.20
-
UW 2×
0.06
7.50
-
Brightfield
10×
0.25
10.50
-
(CFI Plan)
20×
0.40
1.20
0.17
Plan Achromat
0.65
0.56
0.17
50×H
0.90
0.35
-
100×H
1.25
0.20
0.17
No Cover
NCG 40×
0.65
0.48
0
(CFI Plan)
NCG 100×
0.90
0.26
0
(CFI Plan)
0.17
0.2
0.17
DL 4×
0.13
16.40
1.2
DL 10×
0.30
15.20
1.2
DLL 10×
0.30
16.00
-
(CFI Plan Fluor) DLL 20×
DLL 40×
0.50
2.10
0.17
0.75
0.72
0.17
DLL 100×H
1.30
0.20
0.17
2×
0.10
8.50
-
4×
0.20
15.70
-
10×
0.45
4.00
0.17
20×
0.75
1.00
0.17
40×
0.95
0.14
0.11-0.23
Brightfield
40×H
1.00
0.16
0.17
(CFI Plan Apo)
60×
0.95
0.15
0.11-0.23
60×H
1.40
0.21
0.17
VC 60×H
1.40
0.13
0.17
VC 60×WI
1.20
0.27
0.15-0.18
VC 100×H
1.40
0.13
0.17
NCG 100×H
1.40
0.17
0
TIRF 60×H
1.45
0.13
0.10-0.22
1.45
0.13
0.17
DM 20×
0.75
1.00
0.17
DM 40×
0.95
0.14
0.11-0.23
DM 40×H
1.00
0.16
0.17
DM 60×
0.95
0.15
0.11-0.23
DM 60×H
1.40
0.13
0.17
DM 100×H
1.40
0.13
0.17
Evanescent
TIRF
(CFI Plan Apo) TIRF 100×H
DL 10×
0.25
10.50
-
DL 20×
0.40
1.20
0.17
Phase
Contrast
(CFI Plan Apo)
DL 40×
0.65
0.56
0.17
DL 100×H
1.25
0.20
0.17
Fluor
Super Fluor
10×W
0.30
2.00
0
Epi-fl
20×W
0.50
2.00
0
(CFI Fluor)
40×W
0.80
2.00
0
60×W
1.00
2.00
0
4×
0.20
15.5
-
10×
0.50
1.20
0.17
20×
0.75
1.00
0.17
(CFI S Fluor) 40×
0.90
0.30
0.11-0.23
1.30
0.22
0.17
0.5-1.3
0.20
0.17
Epi-fl
Plan Apochromat
UW 1×
40×
0.2
1.2
P 10×
30.00
1.30
0.5-1.3
Phase
Contrast
-
6.20
0.10
100×H
100×H ( iris )
0.17
0.40
4×
100×
0-2.0
0.25
2.10
60×SH ( iris )
0.17
ADL 10×
New Phase LWD ADL 20×F
Contrast
LWD ADL 40×F
(CFI)
0.55
60×
Plan Fluor
0.5-1.25
LWD DL 20×F
DL 40×
Epi-fl
(CFI Plan Fluor) 40×H
0.17
LWD DL 20×
BM 10×A
Phase
Contrast
W.D. Cover-glass
(mm) Thickness
10×
DL 100×H
(CFI)
N.A.
4×
DL 10×
Polarizing
Name
N.A.
100×H( iris)
(CFI)
Use
Name
100×H
Phase
Contrast
W.D. Cover-glass
(mm) Thickness
Type
Type
Use
40×H
100×H( iris)
5
Accessories
Others
Eyepiece Tubes
Name
Beam Split Ratio
Field Number
Binocular Tube B
Observation 100%
22
ISO Photo Tube
Trinocular Tube FUW
Observation 100% / Photo 100%
22/25
Attachable
Trinocular Tube TUW
Observation 100% / Photo 100% / 22/25
Observation 20%, Photo 80%
Attachable
Ergonomic Binocular Tube
Observation 100% /
Observation 50%, Photo 50%
(with DSC port)
22
Eyepiece Lenses
Name (Magnification)
Field Number
CFI 10×
22
CFI 10×M
22
CFI 12.5X
16
CFI 15×
14.5
CFI UW 10×
25
CFI UW 10×M
25
Reticule
Photomask
Photomask
Condensers
Type
N.A.
W.D. (mm)
Magnifications
Abbe Condenser
0.9
1.9
4-100×
Achromat Condenser
0.8
4.2
4-100×
Achromat Swing-out Condenser 2-100X
0.9/0.22
1.8
2-100×
Achromat Swing-out Condenser 1-100X
0.8/0.12
3.2
1-100×
Achromat Aplanat Condenser
1.40
1.6
10-100×
LWD Achromat Condenser
0.65
10.2
4-40×
Low Power Condenser
0.13
10.2
1-4×
Darkfield Condenser (Dry)*
0.8-0.95
4
20-40×
Darkfield Condenser (Oil)*
1.2-1.43
1.5
20-100×
Phase Contrast Condenser*
0.9
1.9
10-100×
*For ECLIPSE 50i
CCTV Adapters
2.5X, 4X relay lenses are ideal for video enhanced contrast (VEC
applications. Use 0.35X, 0.45X and 0.7X relay lenses for 1/3-, 1/2-,
2/3-inch CCD cameras, respectively. 0.9 to 2.2X zoom relay lenses
are also available.
6
Accessories
Teaching Heads ( ECLIPSE 50i )
Teaching heads facilitate simultaneous viewing of the same
specimen without compromising image brightness. A pointer to
show the point of interest is provided. Available from 2-person
(side-by-side or face-to-face) to 5-person types.
2-person (side-by-side) version
2-person (face-to-face) version
Eye-level Riser
Drawing Tube
Can raise the eye-point height in
25mm increments, up to 100mm
maximum depending on the
configuration.
The drawing tube allows both the
image of a specimen and the
drawing to be seen through the
eyepieces. A dedicated adapter is
necessary to attach it.
FX-III Series Photomicrographic Equipment
Double Port
The FX-III series utilizes a directprojection system with a swing-out
prism for fast exposure setting and
accurate metering.
U-III: 0.1% and 1% spot exposure,
and 35% integrated-average
measurement modes
H-III: 1% spot and 35% integratedaverage measurement modes
P-III: Manual exposure model
The double port enables the use
of one CCTV camera and one
digital camera, or two digital
cameras simultaneously.
H-III
Magnification Module
The turret system allows the
intermediate magnification to be
changed to 1X, 1.25X, 1.5X or 2X.
Specimen Holders
Two types of 2-slide holders (opening to the right or left) and a
single-slide holder enabling the quick exchange of the specimen
with one hand are available.
7
Specifications
Main body
50i
55i
Magnification
10 –1500X
Optical system
CFI60 Infinity Optical System
Coarse/fine focusing
Fine: 0.1mm per rotation, Coarse: 13.8mm per rotation, Minimum reading: 1 micron
Coarse motion torque adjustable, Refocusing function (with stopper)
Illumination
6V-30W halogen lamp
100-240V (worldwide voltage)
White LED array
AC adapter (100-240V) or optional lithium battery (7.4V)
Built-in filter
ND8
LA60 color balance filter
Eyepiece tube
Binocular tube B (for F.O.V. 22mm)
Trinocular tube "F" UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 0/100)
Trinocular tube "T" UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 20/80, 0/100)
Ergonomic binocular tube (for F.O.V. 22mm, inclination:10-30°, extension: 40mm); DSC port: 100/0, 50/50 (optional)
Eyepiece lens
10X (F.O.V.: 22mm), 10X M photo mask (F.O.V.: 25mm), 12.5X (F.O.V.: 16mm), 15X (F.O.V.: 14.5mm),
UW 10X (F.O.V.: 25mm), UW 10X M photo mask (F.O.V.: 25mm)
Nosepiece
Sextuple nosepiece
Ergo-View cytodiagnostic unit (Motorized changeover 10X to 40X with hand switch, Stamp type marking
—
Automatic illumination adjustment with Ergo-View
Stage
Super-hard Alumite coated surface, Stay-in-position stage handle, Stage handle height and tension adjustable
Rectangular 159mm X 243mm surface stage, 78mm X 54mm cross travel (x-y movement)
1-slide or 2-slide specimen holder available (option)
Condenser focusing stroke
27mm
Intermediate accessories
Epi-fluorescence illuminator (4 filter positions), Magnification module, Eye-level riser, Double port
Teaching head
Observation method
—
Brightfield, Epi-fluorescence
Darkfield, Phase contrast, Simple polarizing
—
Specifications and equipment are subject to change without any notice
or obligation on the part of the manufacturer. June 2004.
©2003-4 NIKON CORPORATION
WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING
MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
Company names and product names appearing in this brochure are their registered
trademarks or trademarks.
NIKON INSTECH CO., LTD.
NIKON INSTRUMENTS EUROPE B.V.
NIKON INSTRUMENTS INC.
Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku,
Kawasaki, Kanagawa 210-0005, Japan
phone: +81-44-223-2167 fax: +81-44-223-2182
www.nikon-instruments.jp/eng/
P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands
phone: +31-20-44-96-222 fax: +31-20-44-96-298
www.nikon-instruments.com/
1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306
www.nikonusa.com/
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FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33
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GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322
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SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668
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MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387
NIKON CORPORATION
Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku,
Tokyo 100-8331, Japan
www.nikon.com/
Printed in Japan (0406-06)T
Code No. 2CE-MSJH-2
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