Agilent Cary 60 UV-Vis

Agilent Cary 60 UV-Vis
Efficient. Accurate. Flexible.
Guaranteed specifications
The Agilent Cary 60 UV-Vis spectrophotometer is efficient, accurate and flexible, and is
designed to meet both current and future measurement needs. The proven, robust design of
the Cary 60 comprises a double beam, Czerny-Turner monochromator, 190–1100 nm
wavelength range, 1.5 nm fixed spectral bandwidth, full spectrum Xenon pulse lamp single
source with exceptionally long life, dual silicon diode detectors, quartz overcoated optics,
scan rates up to 24,000 nm/min, 80 data points/sec maximum measurement rate, nonmeasurement phase stepping wavelength drive, room light immunity, central control by PC
with Microsoft® Windows® operating system. Supported by GLP software, optional 21 CFR
Part 11 capable software, and dedicated instrument validation software which includes
pharmacopeia test suites.
Agilent Cary 60 UV-Vis spectrophotometers are manufactured according to a quality
management system certified to ISO 9001. The guaranteed specifications are listed in this
document and are based on the 4 sigma statistical confidence level of the final acceptance
tests performed at the factory.
Features, advantages and
Instrument hardware
Xenon pulse lamp source
Eliminates photobleaching while allowing the highest quality data to be
collected over the complete UV-Vis range — all at the lowest cost of
ownership due to an exceptionally long lamp life.
Room light immunity
Unique optical design allows accurate sample measurement even with
the sample lid open — especially useful for enzyme assays, fiber-optic
based measurements or high throughput QA/QC labs.
1.5 nm fixed spectral
Gives excellent spectral resolution for solids and liquids and meets
international Pharmacopoiea compliance regulations.
190–1100 nm wavelength
Complete coverage of UV-Vis range and extending up into the NIR.
24,000 nm/min maximum
scan rate
Allows complete spectral range scanning in under 3 seconds, ideal for
fast kinetics or high sample throughput.
80 data points/second
maximum measurement
Allows accurate measurement of sub-second kinetic reactions with
excellent data fitting.
Photometric range up to
4 Abs
Permits the analysis of highly turbid solutions and a wide range of
sample concentrations (optical densities), as well as reducing sample
preparation (dilution) requirements.
Non-measurement phase
stepping wavelength drive
Means that sample and reference measurements are made at the same
wavelength ensuring that no peak shifts are observed — even when
measuring at the fastest scan speeds.
Focused beam measuring
1.5 x 1.0 mm
Ensures efficient energy coupling to accessories including fiber optic
probes and ultra-microvolume cuvettes for measurement of low volume
Unique full-spectrum Xenon flash lamp (80 Hz) with typical lifetime of
10 years (guaranteed 3 years)
Holographic, 27.5 x 35 mm, 1200 lines/mm, blaze angle 8.6° at 240 nm
Beam splitting system
Beam splitter
2 silicon diode detectors for simultaneous sample beam and reference
beam measurements
Optical design
Double beam Czerny-Turner monochromator
UV-Vis limiting resolution (nm)
≤ 1.5 nm
Toluene/hexane limiting resolution (EP/BP and TGA test)
≥ 1.5
Agilent Cary 60 UV-Vis guaranteed specifications
Instrument hardware
Stray light (%T)
At 198 nm (12 g/L KCl, TGA & BP/EP method)
At 220 nm (10 g/L NaI ASTM method)
At 370 nm (50 mg/L NaNO2)
≤ 1%
≤ 0.05%
≤ 0.05%
Wavelength range (nm)
190–1100 nm
Wavelength accuracy (nm)
± 0.5 at 541.94 nm
Wavelength reproducibility (nm)
± 0.1 nm
Photometric accuracy (Abs)
Using NIST 930E filters at 1 Abs
At 0.2, 0.5 & 0.75 Abs (14.2% w/v KNO3, TGA method)
0.292 to 0.865 Abs (60.06 mg/L K2Cr2O7, BP method)
± 0.005 Abs
± 0.01 Abs
± 0.01 Abs
Photometric range (Abs)
± 4.0 Abs
Photometric display
± 9.9999 Abs, ± 200.00 %T
Photometric reproducibility (Abs)
Using NIST 930E filters, at 465 nm, 2 s SAT
Maximum deviation at 1 Abs
Standard deviation for 10 measurements
Using NIST 930E filters, at 546.1 nm, 2 s SAT
Maximum deviation at 0.5 Abs
Standard deviation for 10 measurements
< 0.004 Abs
< 0.00050 Abs
< 0.003 Abs
< 0.0030 Abs
Photometric stability (Abs/hour)
500 nm, 10 s SAT
< 0.0004 Abs
Photometric noise (Abs, RMS)
500 nm, 1 s SAT
260 nm, 1 s SAT
At 0 Abs
At 1 Abs
At 2 Abs
At 0 Abs
< 0.0001 Abs
< 0.0005 Abs
< 0.005 Abs
< 0.00015 Abs
Baseline flatness (Abs)
200 to 850 nm, smooth 21 filter applied, baseline corrected ± 0.001 Abs
Compartment size (width x depth x height)
130 mm x 523 mm x 123 mm
Note that sample compartment can be left open during measurement
due to room light immunity of Cary 60
Sample compartment access
Top and front
Recommended environmental
Instrument dimensions (width x depth x height)
Packed 595 x 710 x 350 mm (24 x 28 x 14 in)
Unpacked 477 x 567 x 196 mm (19 x 23 x 8 in)
The Cary 60 has been designed to withstand the weight of a PC
monitor up to 10 kg (33 lb)
Instrument weight
Packed 23 kg (51 lb), Unpacked 18 kg (40 lb)
Instrument conditions
Altitude (m, ft)
Temp. (°C, °F)
Humidity (%RH) noncondensing
Non-operating (transport)
0–4600, 0–15000
-40–75 °C, -40–167 °F
Operating within
0–3100, 0–10000
5–40 °C, 41–104 °F
For optimum analytical performance, it is recommended that the ambient temperature of the
laboratory be between 20–25 °C and be held constant to within ±2 °C throughout the entire working
Instrument electrical requirements
A standard 3.2 A/12 V plug pack is provided. Power cords are provided
based on the user’s country requirements. Only the supplied power
supply is to be used with this equipment.
Required supply voltage
100–240 V AC, Frequency 47-63 Hz
Nominal rating
Scanning: 18 W, Idle: 9 W
Spectral bandwidth (nm)
Fixed at 1.5 nm (approximately)
Signal averaging (seconds)
0.0125–999 s
Maximum scan rate (nm/min)
24,000 nm/min
Slew rate (nm/min)
24,000 nm/min
Data interval (nm)
0.15–5.0 nm
Repetitive scanning
4800 data points per minute, maximum number of cycles: 999,
maximum cycle time (min): 9999
Agilent Cary 60 UV-Vis guaranteed specifications
Data collection rate
80 data points/second
Temperature monitor
Temperature probe inside cuvette (using the Temperature Probe
Minimum sample volume
0.5 μL
Customer support policies
Support and training
Agilent is renowned for providing expert applications and service
support. Agilent has a global network of factory-trained specialists
ready to provide support for hardware, software, or applications
wherever you are located. Services include:
• Full 12-month warranty support
• Seven (7) year hardware support period from date of last unit
manufacture. After this time, parts and supplies will be provided if
• Preventive maintenance to deliver consistent operation and minimize
• Troubleshooting, maintenance and repair
• Software support services
• Compliance services including IQ and OQ of hardware and software
• Comprehensive warranty extension and service contracts, including
• Classroom training and onsite training delivered by experts
Further details
More information
For further information please consult your Agilent office or supplier, or
our website at
Agilent shall not be liable for errors contained herein or for incidental or consequential
damages in connection with the furnishing, performance or use of this material.
Information, descriptions, and specifications in this publication are subject to change
without notice.
© Agilent Technologies, Inc. 2011
Published December 7, 2011
Publication number: 5990-7881EN
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