MicroFill Operator`s Manual

Microplate Dispenser
MicroFill
™
Operator’s Manual
.
MicroFill™
Microplate Dispenser, All Models
Operator’s Manual
January 2013
© 2013
Part Number 7141000
Revision G
BioTek® Instruments, Inc.
ii | Preface
Notices
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2013, BioTek® Instruments, Inc. All rights are reserved. No part of this manual may
be reproduced or transmitted in any form, or by any means electronic or mechanical,
including photocopying and recording for any purpose other than the purchaser’s
personal use without written permission of BioTek® Instruments, Inc.
Trademarks
BioTek is a registered trademark, and MicroFill and BioStack are trademarks of BioTek
Instruments, Inc. All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek. Changes made to the information in this document will be
incorporated in new editions of the publication. No responsibility is assumed by
BioTek for the use or reliability of software or equipment that is not supplied by
BioTek or its affiliated dealers.
BioTek Instruments, Inc.
Contents | iii
Contents
Notices ................................................................................. ii
Contact Information ............................................................... ii
Revision History .....................................................................ix
Document Conventions ...........................................................xi
Intended Use Statement ........................................................ xii
Quality Control...................................................................... xii
Warranty and Product Registration........................................... xii
Repackaging and Shipping ...................................................... xii
Warnings ............................................................................ xiii
Hazards .............................................................................. xiii
Precautions ......................................................................... xiv
CE Mark .............................................................................. xvi
Electromagnetic Interference and Susceptibility ....................... xvii
User Safety ....................................................................... xviii
Safety Symbols .................................................................... xix
Chapter 1: Introduction ........................................................ 1
The MicroFill Microplate Dispenser ........................................ 2
Models ......................................................................... 2
Manifolds ..................................................................... 3
Software ...................................................................... 3
Compatibility with the BioStack™ Microplate Stacker .......... 4
Hardware Features............................................................. 5
Package Contents .............................................................. 6
Optional Accessories .......................................................... 7
Specifications .................................................................... 8
Chapter 2: Installation ....................................................... 11
1: Unpack and Inspect the Instrument ................................ 12
2: Select an Appropriate Location....................................... 12
3: Install the MicroFill’s Components .................................. 13
Microplate Carrier ........................................................ 13
Microplate Adaptor ...................................................... 13
Priming Plate .............................................................. 13
Manifold ..................................................................... 14
Syringe Head .............................................................. 15
Check Valves, Tubes, and Bottles .................................. 16
Check Valves .............................................................. 16
MicroFill Operator's Manual
iv | Preface
Supply Tubes ..............................................................
Bottles .......................................................................
Final Check ................................................................
Prime Trough Drain Kit .................................................
Mist Shield .................................................................
Power Supply and Cords ...............................................
Data Communication ...................................................
Serial Port Communications with Other Devices ...............
Operating the MicroFill with the BioStack ........................
4: Verify Performance ......................................................
Repackaging and Shipping Instructions ...............................
Before shipping the instrument: ....................................
Repackaging the instrument and its accessories: .............
17
17
17
19
20
21
21
21
21
22
23
23
23
Chapter 3: Operation .......................................................... 25
Turn On/Off the MicroFill ................................................... 26
Keypad Description .......................................................... 26
Operation with the BioStack .............................................. 28
Main Menu ...................................................................... 28
Program Types ................................................................ 29
Prime Programs ............................................................... 29
Priming Considerations................................................. 29
Define Prime Program .................................................. 31
Define Prime Program Options ...................................... 31
Run Prime Program ..................................................... 31
Edit Prime Program...................................................... 32
Copy Prime Program .................................................... 32
Copy Prime Program Options ........................................ 32
Delete Prime Program .................................................. 32
Delete Prime Program Options ...................................... 32
Dispense Programs .......................................................... 33
Define Dispense Program ............................................. 33
Define Dispense Program Options ............................. 33
Run Dispense Program ................................................. 35
Run Dispense Program Options ................................. 35
Soak Programs ................................................................ 35
Link Programs ................................................................. 36
Define Link Program ............................................................. 36
Define Link Program Options .................................................. 36
Utility Menu Options ......................................................... 37
TESTS ....................................................................... 37
Self-Check ............................................................. 37
BioTek Instruments, Inc.
Contents | v
Configuration Information (CHKSUM) .........................
SETUP .......................................................................
Language ..............................................................
Manifold ................................................................
BIOSTACK ..................................................................
AUTOPRIME ................................................................
Editing the AutoPrime Program .................................
AutoPrime Program Options .....................................
AutoRinse ..............................................................
37
37
37
37
38
38
38
38
39
Chapter 4: Preventive Maintenance .................................... 41
Overview ........................................................................ 42
Recommended Maintenance Schedule for the MicroFill .......... 43
Tools and Supplies ........................................................... 43
Maintenance and Prime Programs ...................................... 44
Daily Maintenance............................................................ 45
Rinse and Soak ........................................................... 45
Removing Protein Residuals and Fungi Growth................. 46
Daily ..................................................................... 46
Weekly/Monthly ...................................................... 47
AUTOPRIME ................................................................ 48
Overnight Maintenance ......................................................... 49
Monthly Maintenance ............................................................ 50
Clean the Bottles and Tubes .............................................. 50
Clean the Manifold ........................................................... 50
Clean (“Stylize”) the Dispense Tubes .................................. 50
Clean the Plate Carrier...................................................... 51
Clean the Plate Carrier Transport Rail ................................. 52
Clean/Replace the Check Valves......................................... 52
Calibrating the Backlash ................................................... 53
Equipment Required .................................................... 53
Setup ........................................................................ 53
Select the Manifold (AF1000A Dispensers Only) ............... 53
Create a Dispense Program........................................... 54
Prime the Plate ........................................................... 54
Calibrate the Backlash ................................................. 54
Autoclaving the Syringe Head ............................................ 56
Long-Term Storage Maintenance ............................................ 57
Storing the MicroFill Dispenser ............................................... 57
Decontamination .................................................................. 58
Purpose .......................................................................... 58
Tools and Supplies ........................................................... 59
MicroFill Operator's Manual
vi | Preface
Decontamination Procedure ............................................... 59
Daily/Overnight Maintenance Log
Monthly Maintenance Log
Chapter 5: Instrument Qualification ................................... 61
IQ/OQ/PQ ....................................................................... 62
Recommended Qualification Schedule ................................. 63
System Self-Check and Checksum Test ............................... 64
System Self-Check ...................................................... 64
Checksum Test ........................................................... 64
Dispense Precision and Accuracy Tests ................................ 64
Overview ................................................................... 64
Specifications ............................................................. 65
Precision .................................................................... 65
Accuracy .................................................................... 65
Materials .................................................................... 66
Test Solutions ............................................................. 67
For 16-Channel Manifold Testing: .............................. 67
For “8-96” Manifold Testing: ..................................... 67
For “8-24” Manifold Testing: ..................................... 67
Dispense Precision & Accuracy Test: 16-Channel Manifold . 69
Dispense Precision & Accuracy Test: “8-96” Manifold ........ 70
Dispense Precision & Accuracy Test: “8-24” Manifold ........ 72
Documenting Test Results ............................................ 74
Liquid Test Worksheets
Performance Verification Logs
Chapter 6: Troubleshooting ................................................ 75
Overview ........................................................................ 76
General Usage Guidelines ................................................. 77
Before Running Any Program: ....................................... 77
Preventive Maintenance ............................................... 77
Programming considerations ......................................... 77
Product Support & Service ................................................ 78
Returning Instruments for Service/Repair ....................... 78
Contacting BioTek for Applications Support ..................... 78
Troubleshooting Charts ..................................................... 79
Dispenser Start-Up ...................................................... 79
Syringe Movement ...................................................... 79
Fluid Delivery ............................................................. 80
Fluid Leakage ............................................................. 81
Microplate Carrier Movement ........................................ 82
BioTek Instruments, Inc.
Contents | vii
Dispense Manifold Movement ........................................ 82
Appendix A: Error Codes ..................................................... 83
Overview ........................................................................ 84
General Errors ................................................................. 84
BioStack Errors ............................................................... 87
Fatal Errors ..................................................................... 88
Appendix B: Chemical Compatibility ................................... 89
Autoclavable MicroFill ....................................................... 90
Table 1. Material/Where Used List .................................. 90
Table 2. Chemical Compatibility Ratings ......................... 91
Non-Autoclavable MicroFill ................................................ 92
Table 3. Material/Where Used List .................................. 92
Table 4. Chemical Compatibility Ratings ......................... 93
Appendix C: Dispense Flow Rates ....................................... 95
Standard (Non-Autoclavable) Versions of the MicroFill™ ........ 96
Autoclavable Versions of the MicroFill ................................. 97
16-Channel Manifold .................................................... 97
“8-96” Manifold ........................................................... 97
“8-24” Manifold ........................................................... 97
Appendix D: Packing Instructions ...................................... 99
MicroFill Operator's Manual
viii | Preface
Contact Information
™ See also “Product Support & Service” in Chapter 6.
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet:
www.biotek.com
Phone:
888-451-5171 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax:
802-655-7941
E-Mail:
customercare@biotek.com
Service/TAC
Phone:
800-242-4685 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax:
802-654-0638
E-Mail:
tac@biotek.com
European Coordination Center/
Authorized European Representative
BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet:
www.biotek.de
Phone:
+49 (0) 7136 9680
Fax:
+49 (0) 7136 968 111
E-Mail:
info@biotek.de
BioTek Instruments, Inc.
Revision History | ix
Revision History
Rev
Date
Changes
A
08/2001
First Issue
B
01/2002
Added new chemical compatibility caution, page v.
Inserted new text throughout manual concerning autoclavable version
of the instrument.
Changed “priming trough” to “priming plate” and “pre-priming trough”
to “priming trough.”
Updated Package Contents and Optional Accessories list.
Revised basecode software to include a “repeat” feature, pages 3-4 and
3-7.
Added new Figures 2-2, 2-4, 4-1 and D-1.
Revised range of prime and dispense flow rates, Chapters 3 and 4.
Added three new appendices C, D, and E.
C
04/2003
Updated contact information and safety symbol text in Preface.
Added information concerning the 24-channel manifold optional
accessory to: Chapters 1, 3, 4; Appendices A and B; Glossary.
Added information regarding the compatibility of µFill Microplate
Dispenser models with the BioStack™ Automated Microplate Stacking
System to: Chapters 1-5, Appendix A, and the Index.
Moved the reference to the serial cable (part no. 75053) from Optional
Accessories to Package Contents, page 1-4.
In Optional Accessories list, indicated which accessories are only used
with the AF1000A model, page 1-4.
Clarified callouts in Figure 2-3, page 2-5.
Under Prime Volume (page 3-7), changed “The volume range is 5 to
1500 µl/well/sec” to “…5 to 1500 µl/well.”
Added a reference to the 8-channel manifold as an option for AF1000A
model users, page 4-8.
Updated error codes in Appendix A.
Added information for blue dye test solution to: Performance
Verification Testing section (page 4-16), and Appendix B, Dye
Preparation for Performance Testing.
Added 8-channel manifold information to: Appendix E, Dispense Flow
Rates.
D
10/2003
Updated Intended Use statement.
E
11/2006
General: Applied new company standard template and logo.
Incorporated contents of Manual Update revision D1.
Preface: Added WEEE directive and symbol. Added pinch point Safety
Symbol. Updated product description under Intended Use. Removed
Warranty.
Ch 1 Introduction: Clarified manifold descriptions (AF1000 and
AF1000A models). Added BioTek part numbers under Package
Contents. Moved Priming Trough Drain Kit from Optional Accessories to
MicroFill Operator's Manual
x | Preface
Rev
Date
Changes
Package Contents.
Ch 2 Installation: Restructured sequence of steps to better reflect
actual practice. Added Prime Trough Drain Kit installation instructions
(from former Appx D).
Ch 3 Operation: Added Utility Menu Options section (moved from
original Ch 4).
Ch 4 Preventive Maintenance: Removed information on Performance
Verification (moved to new Ch 5). Added instructions for Removing
Protein Residuals and Fungi Growth. Moved AutoPrime to Daily
Maintenance section. Enhanced Decontamination procedure and added
step to put priming plate in microplate adaptor. Moved Utility section to
Ch 3. Moved Performance Verification to Ch 5.
Ch 5 Instrument Qualification. New chapter containing previous
Performance Verification information. Enhanced test procedures in line
with µFill IQOQPQ procedure. Added Liquid Test worksheets.
Ch 6 Troubleshooting. Formerly Ch 5. Minor enhancements to
Troubleshooting Charts.
Removed original Appendix B, Dye Preparation for Performance
Testing. Moved this information to Ch 5 Instrument Qualification.
Appx B Chemical Compatibility (formerly Appx C). Updated with latest
information available via BioTek’s website.
Removed original Appendix D, Priming Trough Drain Kit Assembly.
Moved this information to Ch 2.
Appx C Dispense Flow Rates (formerly Appx E). Removed
redundant/inappropriate section “For 8-Channel Manifolds”.
F
2/2008
Throughout: Changed all instances of “BioStack” to “BioStack” and
“µFill” to “MicroFill”.
Preface: Updated the Intended Use Statement. Updated Directives
information and Safety Symbols.
G
1/2013
Preface: Added Repackaging and Shipping statement. Under “Hazards”
added Service, Accessories, Software Quality Control, and Pinch Hazard
warnings. Under “Precautions” added Bovine Serum Albumin, Power
Supply, Electromagnetic Environment, and Electromagnetic
Compatibility precautions. Updated Low Voltage (Safety) Directive and
added EN 61010-2-081. Added EN 61010-2-101 to IVD Directive.
Updated Safety Symbols.
Chapter 2, Installation: Replaced outdated packing instructions with a
reference to new Appendix D.
Added Appendix D, Packing Instructions.
BioTek Instruments, Inc.
Document Conventions | xi
Document Conventions
See also Safety Symbols on page xix:
Example
Description
This icon calls attention to important safety notes.
Warning!
A Warning indicates the potential for bodily harm and
tells you how to avoid the problem.
Caution
A Caution indicates potential damage to the instrument
and tells you how to avoid the problem.
DEFINE
Text in COURIER font represents menu options as they
appear on the instrument’s display.
Note:
Bold text is primarily used for emphasis.
This icon calls attention to important information.
MicroFill Operator's Manual
xii | Preface
Intended Use Statement
BioTek’s MicroFill Microplate Dispenser accommodates 96-, 384- and 24-well standard
microplates and deep well blocks for bulk reagent dispensing applications. An
autoclavable model is available for those applications requiring a sterile fluid path.
The MicroFill can operate with standard robotic systems, such as BioTek’s BioStack
Microplate Stacker.
The intended use of this instrument is dependent on the instrument’s labeling. If there is
an IVD label, then the instrument may be used for clinical, research and development, or
other nonclinical purposes. If there is no such label, then the instrument may only be used
for research and development, or for other nonclinical purposes.
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.
Warranty and Product Registration
Take a moment to review the Warranty information that shipped with your product.
Please also register your product with BioTek to ensure that you receive important
information and updates about the product(s) you have purchased. You can register online
through the Customer Resource Center at www.biotek.com or by calling 888/451-5171 or
802/655-4740.
Repackaging and Shipping
If you need to ship the instrument to BioTek for service or repair,
contact BioTek for a Return Materials Authorization (RMA)
number, and be sure to use the original packing materials. Other
forms of commercially available packaging are not recommended
and can void the warranty. If the original packing materials have
been damaged or lost, contact BioTek for replacement packing.
BioTek Instruments, Inc.
Warnings | xiii
Warnings
Operate the instrument on a level, stable surface away from excessive humidity.
When operated in a safe environment according to the instructions in this
document, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious injury.
See Hazards and Precautions.
Strict adherence to instrument maintenance and qualification procedures is required
to ensure accurate dispense volumes and risk-free operation.
Hazards
The following hazard warnings are provided to help avoid injury:
Warning! Power Rating. The instrument’s power supply must be connected to a
power receptacle that provides voltage and current within the specified rating for
the system. Use of an incompatible power receptacle may produce electrical shock
and fire hazards.
Warning! Electrical Grounding. Never use a plug adapter to connect primary
power to the power supply. Use of an adapter disconnects the utility ground,
creating a severe shock hazard. Always connect the system power cord directly to
an appropriate receptacle with a functional ground.
Warning! Service. Only qualified technical personnel should perform service
procedures on internal components.
Warning! Accessories. Only accessories which meet the manufacturer’s
specifications shall be used with the instrument.
Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard or instrument damage. If a
spill occurs while a program is running, abort the program and turn the instrument
off. Wipe up all spills immediately. Do not operate the instrument if internal
components have been exposed to fluid.
Warning! Unspecified Use. Failure to operate the equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.
Warning! Software Quality Control. The operator must follow the
manufacturer’s assay package insert when modifying software parameters and
establishing dispensing methods. Failure to conduct quality control checks could
result in erroneous test data.
Warning! Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.
MicroFill Operator's Manual
xiv | Preface
Warning! Potential Biohazards. Some assays or specimens may pose a
biohazard. Adequate safety precautions should be taken as outlined in the assay’s
package insert. Always wear safety glasses and appropriate protective equipment,
such as chemically resistant rubber gloves and apron.
Warning! Pinch Hazard. Do not reach under the instrument during operation; the
syringe pump may pinch your fingers.
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service. The instrument should be serviced by BioTek authorized service
personnel. Only qualified technical personnel should perform troubleshooting and
service procedures on internal components.
Caution: Spare Parts. Only approved spare parts should be used for
maintenance. The use of unapproved spare parts and accessories may result in a loss
of warranty and potentially impair instrument performance or cause damage to the
instrument.
Caution: Environmental Conditions. Do not expose the instrument to
temperature extremes. For proper operation, ambient temperatures should remain
withing the range listed in the Specification section. Performance may be
adversely affected if temperatures fluctuate above or below this range. Storage
temperature limits are broader.
Caution: Sodium Hypochlorite. Do not expose any part of the instrument to
sodium hypochlorite solution (bleach) for more than 20 minutes. Prolonged contact
may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all
surfaces.
Caution: Buffer Solution. Although many precautions have been taken to ensure
that the instrument is as corrosion-proof as possible, the dispenser is not sealed and
liquids can seep into sensitive components. Make sure that any spilled buffer
solution is wiped off the dispenser. Continuous exposure to salt solution may
corrode parts of the microplate carrier, movement rail, springs, and other hardware.
Caution: Chemical Compatibility. Some chemicals may cause irreparable
damage to this instrument. The following chemicals have been deemed safe for use
in this instrument: buffer solutions (such as PBS), saline, surfactants, deionized
water, 70% ethyl, isopropyl, or methyl alcohol, 40% formaldehyde, and 20% sodium
hydroxide. Other chemicals may cause severe damage to the instrument. Contact
BioTek Instruments if you require further assistance. Refer to Appendix B,
Chemical Compatibility.
BioTek Instruments, Inc.
Precautions | xv
Caution: Bovine Serum Albumin. Solutions containing proteins, such as bovine
serum albumin (BSA), will compromise the instrument’s performance over time
unless a strict maintenance protocol is adhered to.
Caution: Power Supply. Only use the power supply shipped with the instrument,
and operate it within the range of line voltages listed on it.
Caution: Disposal. This instrument contains printed circuit boards and wiring
with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on
waste electrical and electronic equipment (WEEE),” or local ordinances.
Caution: Warranty. Failure to follow preventive maintenance protocols may void
the warranty. See Chapter 4, Preventive Maintenance.
Caution: Electromagnetic Environment. Per EN 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.
Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper operation.
MicroFill Operator's Manual
xvi | Preface
CE Mark
Based on the testing described below and information
contained herein, this instrument bears the CE mark.
™ See the Declaration of Conformity for more specific information.
Directive 2004/108/EC Electromagnetic Compatibility
Emissions - CLASS A
EN50081-1 and IEC 61326-1
EN 55022 Class A
This equipment has been designed and tested to CISPR 11 Class A. In a domestic
environment it may cause radio interference, in which case, you may need to
mitigate the interference.
Immunity
EN 50082-1 and IEC 61326-1
IEC 1000-4-2 Electrostatic Discharge
IEC 1000-4-3 Radiated EM Fields
IEC 1000-4-4 Electrical Fast Transient/Burst
IEC 1000-4-5 Surge Immunity
EN 61000-4-6 Conducted Disturbances
EN 61000-4-11 Voltage Dips, Short Interruptions and Variations
Directive 2006/95/EC Low Voltage (Safety)
The system has been type-tested by an independent testing laboratory and was found
to meet the requirements of this Directive. Verification of compliance was conducted to
the limits and methods of the following:
EN 61010-1, “Safety requirement for electrical equipment for measurement, control and
laboratory use. Part 1, General requirements.”
EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory
equipment for analysis and other purposes.”
Directive 2002/96/EC Waste Electrical and Electronic Equipment
Disposal Notice: This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste
electrical and electronic equipment (WEEE)” or local ordinances.
BioTek Instruments, Inc.
Electromagnetic Interference and Susceptibility | xvii
Directive 98/79/EC In Vitro Diagnostics (if labeled for this use)
Product registration with competent authorities.
EN 61010-2-101, “Particular requirements for in vitro diagnostic (IVD) medical
equipment.”
Electromagnetic Interference and Susceptibility
USA FCC CLASS A
RADIO AND TELEVISION INTERFERENCE
NOTE: This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can
radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference,
in which case the user will be required to correct the interference at their own expense.
In order to maintain compliance with FCC regulations shielded cables must be used
with this equipment. Operation with non-approved equipment or unshielded cables is
likely to result in interference to radio and television reception.
Canadian Department of Communications Class A
This digital apparatus does not exceed Class A limits for radio emissions from digital
apparatus set out in the Radio Interference Regulations of the Canadian Department of
Communications.
Le present appareil numerique n'émet pas de bruits radioélectriques depassant les
limites applicables aux appareils numérique de la Class A prescrites dans le Réglement
sur le brouillage radioélectrique édicté par le ministère des Communications du
Canada.
MicroFill Operator's Manual
xviii | Preface
User Safety
This device has been type-tested by an independent laboratory and found to meet the
requirements of the following:
•
Underwriters Laboratories UL 61010-1, “Safety requirements for electrical
equipment for measurement, control and laboratory use; Part 1: General
requirements.”
•
Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety
requirements for electrical equipment for measurement, control and laboratory
use; Part 1: General requirements.”
•
EN 61010 Standards, see CE Mark starting on page xvi.
BioTek Instruments, Inc.
Safety Symbols | xix
Safety Symbols
Some of these symbols may appear on the instrument or accessories:
Alternating current
Both direct and alternating current
Courant alternatif
Courant continu et courant alternatif
Wechselstrom
Gleich - und Wechselstrom
Corriente alterna
Corriente continua y corriente alterna
Corrente alternata
Corrente continua e corrente alternata
Direct current
Earth ground terminal
Courant continu
Borne de terre
Gleichstrom
Erde (Betriebserde)
Corriente continua
Borne de tierra
Corrente continua
Terra (di funzionamento)
On (Supply)
Protective conductor terminal
Marche (alimentation)
Borne de terre de protection
Ein (Verbindung mit dem Netz)
Schutzleiteranschluss
Conectado
Borne de tierra de protección
Chiuso
Terra di protezione
Off (Supply)
Caution (refer to accompanying documents)
Arrêt (alimentation)
Attention (voir documents
d’accompagnement)
Aus (Trennung vom Netz)
Desconectado
Achtung siehe Begleitpapiere
Aperto (sconnessione dalla
rete di alimentazione)
Atención (vease los documentos incluidos)
Warning, risk of electric shock
Warning, risk of crushing or pinching
Attention, risque de choc
électrique
Attention, risque d’écrasement et
pincement
Gefährliche elektrische schlag
Warnen, Gefahr des Zerquetschens und
Klemmen
Precaución, riesgo de sacudida
eléctrica
Attenzione, rischio di scossa
elettrica
Attenzione, consultare la doc annessa
Precaución, riesgo del machacamiento y
sejeción
Attenzione, rischio di schiacciare ed
intrappolarsi
Warning, hot surface
Warning, potential biohazards
Attention, surface chaude
Attention, risques biologiques potentiels
Warnen, heiße Oberfläche
Warnung! Moegliche biologische Giftstoffe
Precaución, superficie caliente
Atención, riesgos biológicos
Attenzione, superficie calda
Attenzione, rischio biologico
MicroFill Operator's Manual
xx | Preface
In vitro diagnostic medical
device
Separate collection for electrical and
electronic equipment
Dispositif médical de
diagnostic in vitro
Les équipements électriques et
électroniques font l’objet d’une collecte
sélective
Medizinisches In-VitroDiagnostikum
Dispositivo médico de
diagnóstico in vitro
Dispositivo medico diagnostico
in vitro
Getrennte Sammlung von Elektro- und
Elektronikgeräten
Recogida selectiva de aparatos eléctricos y
electrónicos
Raccolta separata delle apparecchiature
elettriche ed elettroniche
Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de
uso
Consultare le istruzioni per uso
BioTek Instruments, Inc.
Chapter 1
Introduction
This chapter introduces the MicroFill™ Microplate Dispenser, and
describes its hardware and software features.
The MicroFill Microplate Dispenser ............................................ 2
Models ............................................................................. 2
Manifolds .......................................................................... 3
Software .......................................................................... 3
Compatibility with the BioStack™ Microplate Stacker .............. 4
Hardware Features ................................................................. 5
Package Contents ................................................................... 6
Optional Accessories ............................................................... 7
Specifications ........................................................................ 8
2 | Chapter 1: Introduction
The MicroFill Microplate Dispenser
The MicroFill™ is a self-contained, user-programmable, universal microplate dispenser.
The following unique combination of features expands applications far beyond the typical
microplate dispenser:
•
The microprocessor-controlled syringe pump with its long-lasting seal ensures
precise and accurate fluid delivery, as well as reproducibility for repeated
dispenses.
•
The low-profile, robot-compatible carrier accommodates deep-well microplates
and microtubes in 24-, 96-, and 384-well formats. A microplate adaptor
accommodates standard height plates.
•
User-programmable dispense rates and depths provide for a wide range of
dispensing capabilities.
•
The horizontal dispense position provides for precise positioning of the wells
under the dispense tubes.
•
The MicroFill™ can be used to dispense a variety of solutions, including buffered
saline and reagent solutions.
•
The intuitive menu-driven software allows you to create and store up to 100
dispense programs. When you are ready to run a program, simply select the
program name and enter the starting strip and number of strips. The MicroFill will
then process the plate according to the program parameters.
•
Several predefined programs are provided to simplify preventive maintenance,
which should be performed regularly to ensure optimum dispenser performance.
•
The MicroFill™ has a low-maintenance design, the result of BioTek’s long history
with liquid-handling instruments. The MicroFill™ does not require recalibration.
•
In addition to operating on a stand-alone basis, the MicroFill™ is compatible with
BioTek’s BioStack™ Microplate Stacker and BioStack Twister II Microplate
Handler.
Models
•
The AF1000 supports the 16-channel manifold, for standard 96- and 384-well
plates. It is not autoclavable and should not be used with organic solvents.
•
The AF1000A is autoclavable and can be used with organic solvents.
It supports the following manifolds:
¾
16-channel, for standard 96-/384-well plates
¾
8-channel for 96-well standard, half-area, and PCR plates (“8-96”)
¾
8-channel for standard 24-well plates (“8-24”)
BioTek Instruments, Inc.
The MicroFill Microplate Dispenser | 3
Manifolds
The manifold is the component that delivers fluid to the microwells. Each dispenser is
equipped with a manifold containing 16 dispense tubes for dispensing into 96- and
384-well plates. There is one tube per well for 384-well plates and two tubes per well
for 96-well plates.
For the autoclavable model (AF1000A), an optional “8-96” manifold is available for
dispensing into 96-well half-area and PCR plates. An optional “8-24” manifold is
available for dispensing into standard 24-well plates.
See page 8 for information on microplate types and dimensions supported by the
different manifolds.
Software
The dispenser is operated by onboard software, controlled through the dispenser’s
keypad. This onboard software, referred to as the “basecode,” has an identifying part
number.
Your dispenser is installed with basecode part number 7140200. The basecode also has
an identifying version number. If you need to contact BioTek Instruments for
technical assistance, you will be asked for the part number and version of the basecode
currently residing on your dispenser. To locate this information, press these keys from
the Main Menu: UTIL > TESTS > CHKSUM.
The basecode part number and accompanying version number will appear for a few
seconds, followed by additional configuration information. The display will
automatically return to the main menu.
The 7140200 basecode supports the following features:
•
An easy to use, menu-driven interface.
•
Ability to create Prime, Dispense, and Linked programs for automated
dispensing to microplates.
•
Storage of up to 100 programs.
•
User-definable program names, for rapid recall at program run time.
•
Configurable program parameters such as variable dispense flow rates,
dispense heights, and starting strip.
•
Predefined Maintenance programs, including AutoPrime, that are used to
keep the manifold tubes moist and free from clogging.
•
Support for the following languages: English, French, German, Italian, and
Spanish. To switch to a different language, press these keys from the Main
Menu: UTIL > SETUP > LANGUAGE.
MicroFill Operator's Manual
4 | Chapter 1: Introduction
Compatibility with the BioStack™ Microplate Stacker
The MicroFill™ is compatible with BioTek’s BioStack Microplate Stacker. The basecode
software of the MicroFill has been modified so that its normal operating displays
include the BioStack options. Upgrade kits are available for customers who purchased
their MicroFill before modifications to the basecode. (The MicroFill’s basecode version
must be 1.10 or greater to operate with the BioStack.)
Controlled serially by the MicroFill, the BioStack can rapidly and systematically
transfer microplates one at a time, to and from the dispenser, and includes:
•
Removable stacks (one input and one output) that can hold up to 30
microplates each.
•
Optional restacking of plates to maintain correct sequencing.
•
The ability to continue processing a stack of plates following the aborting/
failure of one plate.
•
The ability to pause processing to add more plates to the input stack or to
remove some from the output stack.
If you have purchased the BioStack to operate with the MicroFill, refer to the BioStack
Operator’s Manual for instructions on configuring the dispenser to run with the
BioStack. If you are interested in purchasing the BioStack, contact your local BioTek
dealer for more information.
BioTek Instruments, Inc.
Hardware Features | 5
Hardware Features
•
2-line x 24-character LCD display.
•
Membrane keypad with 25 alphanumeric keys.
•
Dispenses to 96-well microplates with 0.355" (9 mm) well centers.
•
Dispenses to 384-well microplates with 0.177" (4.5 mm) well centers.
•
Dispenses to 24-well microplates with 0.582" (14.78 mm) well centers.
•
External 24-volt power-supply is compatible with 100 – 240 V~; 50 - 60 Hz.
•
One serial RS-232 COM port (25-pin male connector).
•
Two 2-liter supply bottles; the autoclavable instrument has two 1-liter glass supply
bottles.
•
Controllable flow rates and volumes provided by a positive displacement syringe
pump. Settings are adjustable via the onboard software, for low- to high-velocity
dispensing.
•
Stepper motors provide accurate and repeatable carrier and manifold positioning.
•
Dispenses to flat, round and “V” bottomed strips and plates in standard and deepwell depths.
•
Carrier is easily removed for cleaning.
•
Priming/soaking trough built into the carrier. A prime trough drain kit is supplied
as an accessory.
•
Mist shield protects the user from aerosols.
MicroFill Operator's Manual
6 | Chapter 1: Introduction
Package Contents
™ Package contents and part numbers are subject to change. Please
contact BioTek Customer Care with any questions.
•
Power supply (PN 61062)
•
Power cord (PN varies according to country of use)
•
Priming plate (PN 7142002)
•
RS-232 serial cable (PN 75053)
•
Operator’s Manual (PN 7141000)
•
16-channel manifold (PN 7140519 for nonautoclavable model; PN 7140508 for
autoclavable model)
•
Two thumbscrews for attaching the manifold (PN 12174)
•
Microplate carrier (PN 7140501)
•
Microplate adaptor (PN 7140504)
•
Two 2-liter supply bottles (PN 4070515) (autoclavable instrument includes two
1-liter glass supply bottles, PN 7140511)
•
Supply tubing set (PN 7140505 for non-autoclavable; 7140510 for autoclavable)
•
Mist shield (PN 4070517)
•
Two thumbscrews for attaching the mist shield (PN 19965)
•
Stylus (PN 2872304)
•
Prime trough drain kit (PN 7140006)
•
Autoclavable instrument includes 3/32” (2.38 mm) hex wrench (PN 48570)
BioTek Instruments, Inc.
Optional Accessories | 7
Optional Accessories
™ Accessory availability and part numbers are subject to change. Please
contact BioTek Customer Care if you have any questions, or visit
www.biotek.com and use the Accessories search tool.
•
“8-96” autoclavable manifold for 96-well half-area and PCR plates (BioTek part
number 7140515S)*
•
“8-24” autoclavable manifold for 24-well plates (BioTek part number 7140518S)*
•
PCR plate adaptor (BioTek part number 6002076)**
•
Supply tubing set for dispensing organic solvents, such as DMSO and Acetonitrile
(BioTek part number 7140514)*
•
Spare autoclavable syringe head with piston (BioTek part number 7140516)*
•
Spare standard 16-channel manifold (BioTek part number 7140519S)
•
Spare autoclavable 16-channel manifold (BioTek part number 7140508S)*
•
Spare standard tubing set (BioTek part number 7140505)
•
Spare autoclavable tubing set (BioTek part number 7140510)*
•
ActiveX® programmer’s package (BioTek part number 7140202)
* For use with autoclavable model AF1000A only
** For use in conjunction with part number 7140515S
MicroFill Operator's Manual
8 | Chapter 1: Introduction
Specifications
Labware
Microplates:
96-well, 384-well, standard, low profile, or deep-well depth
Standard 24-well, with outside dimensions of 5.030” ± 0.010” x
3.365” ± 0.010” (127.76 mm ± 0.25 mm x 85.47 mm ± 0.25 mm)
Microstrips:
1x8
Microwells:
Flat, round, “V” bottom, PCR, or half-area
Dispense Precision and Accuracy Performance
The MicroFill™ Microplate Dispenser is factory-tested using dispense volumes of
80 µl and 20 µl.
Precision:
≤ 2%CV typical when dispensing 100 µl of deionized water with
FD&C #1 blue or #5 yellow dye, at dual wavelength using the
appropriate wavelength for the dye
< 5%CV (typical precision at 20 µl per tube)
< 10%CV (typical precision at 5 µl per tube)
Accuracy,
16-Channel
Manifolds:
384-Well Dispense
1 µl for volumes of 5 to 100 µl, or 1% for all other volumes
96-Well Dispense
2 µl for volumes of 10 to 200 µl, or 1% for all other volumes
Accuracy,
8-Channel
Manifolds:
96-Well Dispense
2 µl for volumes of 10 to 20 µl
1.5 µl for volumes of 21 to 40 µl
1 µl for volumes of 41 to 100 µl
1% typical for all other volumes when dispensing deionized
water into 96-well plates
24-Well Dispense
4 µl for volumes of 20 to 40 µl
3 µl for volumes of 41 to 80 µl
2 µl for volumes of 81 to 200 µl
1% typical for all other volumes when dispensing deionized
water into 24-well plates
BioTek Instruments, Inc.
Specifications | 9
Hardware & Environmental
Manifold
Types:
16-channel non-autoclavable
16-channel autoclavable
8-channel autoclavable for dispensing to 96-well plates (“8/96”)
8-channel autoclavable for dispensing to 24-well plates (“8/24”)
User
Interface:
2-line x 24 character LCD screen
25 alphanumeric soft keys
Power
Supply:
The instrument is operated from a 24-volt external power supply
that is compatible with 100-240 V~; 50-60 Hz.
Dimensions:
Instrument only: 18 in. x 15 in. x 7 in. (46 cm x 38 cm x 18 cm)
Weight:
20 lbs. (8.9 kg)
Operating
Conditions:
10° - 40°C (50° - 104°F)
Relative
Humidity:
The instrument should be operated in a noncondensing humid
environment having a maximum relative humidity of 80% at
temperatures up to 31° decreasing linearly to 50% relative
humidity at 40°C.
MicroFill Operator's Manual
10 | Chapter 1: Introduction
BioTek Instruments, Inc.
Chapter 2
Installation
This chapter includes instructions for setting up the MicroFill™
Microplate Dispenser, and for installing its components.
1: Unpack and Inspect the Instrument ....................................
2: Select an Appropriate Location ...........................................
3: Install the MicroFill’s Components .......................................
Microplate Carrier ............................................................
Microplate Adaptor ...........................................................
Manifold .........................................................................
Syringe Head ..................................................................
Check Valves, Tubes, and Bottles .......................................
Prime Trough Drain Kit .....................................................
Mist Shield ......................................................................
Power Supply and Cords ...................................................
Data Communication ........................................................
Operating the MicroFill™ with the BioStack ..........................
4: Verify Performance ...........................................................
Repackaging and Shipping Instructions ...................................
Before shipping the instrument: .........................................
Repackaging the instrument and its accessories: ..................
12
12
13
13
13
14
15
16
19
20
21
21
21
22
23
23
23
12 | Chapter 2: Installation
1: Unpack and Inspect the Instrument
Important! Save all packaging materials. If you need to ship
the instrument to BioTek for repair or replacement, you must
use the original packing materials. Using other forms of
commercially available packing materials, or failing to follow
the repackaging instructions, may void your warranty.
If the original packing materials have been damaged,
replacements are available from BioTek.
Inspect the shipping box, packaging, instrument, and accessories for signs of damage.
If the MicroFill is damaged, notify the carrier and your manufacturer’s representative.
Keep the shipping cartons and packing material for the carrier’s inspection. BioTek will
arrange for repair or replacement of your instrument immediately, before the shippingrelated claim is settled.
See page 23 for complete packaging and shipping instructions.
2: Select an Appropriate Location
Install the Dispenser on a level surface in an area where ambient temperatures between
10°C (50°F) and 40°C (104°F) can be maintained.
The Dispenser is sensitive to extreme environmental conditions. Avoid excessive
humidity. Condensation directly on the sensitive electronic circuits can cause the
instrument to fail internal self-checks. See the environmental specifications in Chapter 1.
BioTek Instruments, Inc.
3: Install the MicroFill’s Components | 13
3: Install the MicroFill’s Components
Important! Refer to Figure 1 on page 14 when installing
the microplate carrier, microplate adaptor, and dispense
manifold.
Microplate Carrier
•
Hold the microplate carrier over the rail so that its priming trough is closest to the
rear of the dispenser.
™ Note: The priming trough is used if a short prime is programmed as a
part of a dispense program, catching any drips from the manifold that
may fall when the syringe pump is homed. It also serves as an
alternate priming location when the MicroFill™ is integrated into a
robotic system.
•
Line up the pin on the underside of the carrier with the slot on the carrier
transport.
•
Snap the two carrier clips onto the carrier transport rail. The pin should sit in the
slot.
Microplate Adaptor
•
Insert the microplate adaptor into the carrier. Ensure that the adaptor is fully
seated in the carrier.
™ Note: The microplate adaptor must be in place when using standard
height microplates and when priming the dispenser. For dispensing
into deep-well plates, the microplate adaptor must be removed.
Priming Plate
The supplied priming plate is used when running Prime and Maintenance
programs (see “Prime Programs” in Chapter 3). The priming plate sits inside the
microplate adaptor, and it has a partition that divides the plate into two
compartments. See Figure 9 in Chapter 3.
•
When priming the instrument, the priming plate should be oriented with the
large compartment under the manifold.
•
When running a maintenance program, the priming plate should be oriented
with the small compartment under the manifold.
MicroFill Operator's Manual
14 | Chapter 2: Installation
Manifold
•
Orient the manifold so that the tubes are pointed down, and the fitting is pointed
toward the rear of the dispenser.
•
Slide the manifold gently down over the two support pins closest to the front of the
dispenser.
•
Insert the thumbscrews (2). Do not over-tighten.
Thumbscrews (2)
Microplate
Adaptor
REAR
Manifold
Keypad
Priming
Trough
FRONT
Rail
Glide Strip
(Do not remove)
Leveling
Screws
Carrier
Transport
Microplate
Carrier
Figure 1: Installing the MicroFill’s components
BioTek Instruments, Inc.
3: Install the MicroFill’s Components | 15
Syringe Head
For autoclavable versions of the instrument, model AF1000A, only.
To install the syringe head onto the syringe drive at the rear of the instrument:
1.
With the flat side of the shaft facing up, slide the syringe piston shaft into
the piston holder until it stops.
2.
Use the 3/32” (2.38 mm) hex wrench to tighten the setscrew down onto the
flat side of the shaft.
3.
Push the syringe head onto the alignment pins and use the hex wrench to
attach the two mounting screws.
4.
A small magnet is provided for mounting the hex wrench to the base of the
Dispenser for storage. Choose a convenient location for the magnet and attach
the wrench to it.
Setscrew
Syringe piston shaft,
flat side up
Piston holder
(Syringe drive
component)
Syringe head
Mounting screws (2)
Figure 2: Dispenser rear panel, syringe head installation (autoclavable version)
MicroFill Operator's Manual
16 | Chapter 2: Installation
Check Valves, Tubes, and Bottles
Important! Review Figure 3 through Figure 5 on the
following pages, as well as the diagram on the back of the
dispenser. Identify the various tubes and bottles that came
with your dispenser.
The supply bottles have Luer fittings. Finger-tighten only!
Check Valves
Two check valves are supplied; note the flow direction arrows. Connect the check
valves to the syringe pump’s top and bottom ports. Compare the flow direction
arrows with those shown below.
Syringe pump top port,
supply outlet
Upper check valve;
note direction of flow
Power cord
connector
Syringe pump
bottom port,
supply inlet
Serial port
connector
Lower check valve;
note direction of flow
Figure 3: Dispenser rear panel, check valve installation
™ Note: If the check valves are replaced, it may be necessary to
recalibrate the syringe backlash to achieve optimum accuracy
performance. See “Calibrating the Backlash” in Chapter 4 for more
information.
BioTek Instruments, Inc.
3: Install the MicroFill’s Components | 17
™ Note: It is difficult to see the flow direction arrows on the autoclavable
check valves. The valves are made of a translucent plastic and the flow
direction can be determined by looking through the wall of the outer
shell of the check valve. One end of the check valve has two
red/orange o-rings; the other end has one red/orange o-ring and a
gray spring. The direction of flow is from the end having one o-ring to
the end having two o-rings. See below.
Direction of flow
on check valves
One o-ring on this side,
red/orange in color
Two o-rings on this side,
red/orange in color
Figure 4: Autoclavable check valve, detail
Supply Tubes
Connect the check valves as shown in Figure 3. The upper check valve attaches to
the dispense manifold (shorter tube); the lower check valve attaches to the fluid
supply bottle (longer tube). See Figure 5.
Bottles
•
Rinse all bottles with deionized or distilled water before using. This rinsing
eliminates particles that may have entered during packing or unpacking.
•
Place the supply bottles on the same horizontal plane as the dispenser. This
ensures optimum pump performance.
Final Check
•
Verify that the tubing was not crimped during installation.
•
If the mounted manifold tilts forward or backward, make sure that the tubing
to the manifold is not being pulled tight or off to the side.
MicroFill Operator's Manual
18 | Chapter 2: Installation
Long Supply Tube
Luer Fitting
Short Supply Tube
Supply Bottle
Microplate
Carrier
On/Off Switch
Figure 5: Tubing connections
™ Note: The dispenser’s top shroud is removed from this figure for
clarity only. While it is acceptable to remove the mist shield (see page
20), do not attempt to remove the top shroud which protects the
dispenser’s internal components.
BioTek Instruments, Inc.
3: Install the MicroFill’s Components | 19
Prime Trough Drain Kit
Refer to the figure below when installing the prime trough drain kit.
Priming Trough
Drain Plug
Threaded
Fitting
Clamp
Tubing
Priming Trough Drain Instructions
1 . Remove drain plug and install the threaded fitting.
2. Move carrier all the way forward and install tubing onto
the barbed fitting on the back of the priming trough.
3. Route tubing as shown with a little spare tubing
in the back. Apply the clamp approximately as
shown. Then place the tubing into the clamp.
Figure 6: Priming Trough Drain Kit, installation instructions
MicroFill Operator's Manual
20 | Chapter 2: Installation
Mist Shield
1.
Insert the two thumbscrews into the slots on the dispenser’s left side. Do not
tighten completely.
2.
Hold the mist shield parallel to the dispenser’s left side, with the door toward
the front of the dispenser.
3.
Slide the mist shield down so that the two slots fit over the thumbscrew shafts.
4.
Fit the two pins into the slots on the dispenser’s top left side.
5.
Tighten the thumbscrews.
Top shroud
(do not remove)
Pins (2)
Mist shield
Thumbscrews (2)
Figure 7: Attaching the mist shield
BioTek Instruments, Inc.
3: Install the MicroFill’s Components | 21
Power Supply and Cords
The MicroFill™ uses an external 24-volt power supply that is compatible with 100-250
V~; 50-60 Hz. Connect the power cord to the power supply, then connect the power
supply’s output plug to the 24-VDC connector on the rear of the instrument. Plug the
3-prong end of the power cord into an appropriate power receptacle.
Warning! Power Rating. The MicroFill™ must be
connected to a power receptacle that provides voltage
and current within the specified rating for the system.
Use of an incompatible power receptacle may produce
electrical shock and fire hazards
Data Communication
Serial Port Communications with Other Devices
The MicroFill™ has a 25-pin serial (RS-232) port located on the rear panel of the
instrument (see Figure 3). The serial port allows the dispenser to communicate
with the BioStack™ Microplate Stacker, to receive data from a computer for
purposes of factory or dealer service, and/or to be operated using an ActiveX®
interface (contact your BioTek representative for more information on obtaining a
programmer’s package).
Operating the MicroFill™ with the BioStack
Alignment hardware and serial cable connections need to be installed to operate the
MicroFill with the BioStack™ Microplate Stacker. Refer to your BioStack Operator’s
Manual for installation instructions.
MicroFill Operator's Manual
22 | Chapter 2: Installation
4: Verify Performance
Before using the MicroFill™ for the first time, verify that it is operating properly by
turning the dispenser on. The power switch is located on the dispenser’s left side.
Each time the dispenser is turned on, it automatically performs a system self-test. This test
checks the carrier, manifold lift positioning, and syringe pump positioning and operation.
If the test passes, the Main Menu appears and the dispenser is ready for use.
If the self-test fails, the dispenser will “chirp” and display an error code. Press the Stop
button on the keypad and then look up the code in Appendix A, Error Codes.
™ Note: You can also run this test from the dispenser’s front panel, by
selecting UTIL > TESTS > SELFCHK.
For information on performance verification and IQ/OQ/PQ procedures, see Chapter 5,
Instrument Qualification.
Important! Before operating the dispenser, review the
General Usage Guidelines in Chapter 6. These
guidelines include necessary steps to perform before
running any program, information on performing
preventive maintenance,
BioTek Instruments, Inc.
Repackaging and Shipping Instructions | 23
Repackaging and Shipping Instructions
If you need to ship the MicroFill to BioTek for service or repair, be sure to use the original
packing materials. Other forms of commercially available packing are not recommended
and can void the warranty.
If the original packing materials have been damaged or lost, contact BioTek Customer
Service for replacement materials.
If the MicroFill has been exposed to potentially
hazardous material, decontaminate it to minimize the
risk to all who come in contact with the Dispenser during
shipping, handling and servicing.
Decontamination prior to shipping is required by the U.S.
Department of Transportation regulations.
Before shipping the instrument:
•
Decontaminate the dispenser and its accessories as necessary (see Chapter 4).
•
Obtain a Return Materials Authorization (RMA) number from BioTek TAC.
Mark this number on the outside of the shipping box.
•
Include instructions regarding what is required of the BioTek Service
Department. Clearly state whether the unit requires calibration, cleaning, periodic
maintenance, warranty work, and/or repair.
•
Provide BioTek with the name and telephone number of a person who may be
contacted if questions arise.
•
Insure the instrument for full value.
Repackaging the instrument and its accessories:
Refer to the Customer Packing Instructions in Appendix D.
MicroFill Operator's Manual
24 | Chapter 2: Installation
BioTek Instruments, Inc.
Chapter 3
Operation
This chapter includes instructions for operating the MicroFill™
Microplate Dispenser and its on-board software.
Turn On/Off the MicroFill™ ....................................................
Keypad Description...............................................................
Operation with the BioStack ...................................................
Main Menu ..........................................................................
Program Types ....................................................................
Prime Programs ...................................................................
Priming Considerations .....................................................
Define Prime Program ......................................................
Run Prime Program ..........................................................
Edit Prime Program ..........................................................
Copy Prime Program ........................................................
Delete Prime Program ......................................................
Dispense Programs ...............................................................
Define Dispense Program ..................................................
Run Dispense Program .....................................................
Soak Programs ....................................................................
Link Programs .....................................................................
Define Link Program .........................................................
Utility Menu Options .............................................................
TESTS ............................................................................
SETUP ............................................................................
AUTOPRIME ....................................................................
26 26 28 28 29 29 29 31 31 32 32 32 33 33 35 35 36 36 37 37 37 38 26 | Chapter 3: Operation
Turn On/Off the MicroFill™
To turn the MicroFill™ on, press the On/Off switch on the dispenser’s right side. The
keypad will remain inactive while the dispenser performs a self-test. If the test passes, the
Main Menu will appear and the dispenser is ready for use.
If the self-test fails, the dispenser will “chirp” and display an error code. Press the Stop
key and look up the code in Appendix A, Error Codes. See also Chapter 6,
Troubleshooting.
To turn the MicroFill™ off, press the On/Off switch on the dispenser’s right side.
™ Note: To rinse, soak, or decontaminate the dispenser before turning it
off, see Chapter 4, Preventive Maintenance.
Keypad Description
The MicroFill features a 25-key keypad and a 2-line x 24-character LCD display as the user
interface. The keypad is shown below.
LCD
µFill Reagent Dispenser
RUN
DEFINE
MAINT
UTIL
Softkeys:
Press a key
to choose
a menu
function
Menu
functions
Press the left
and right arrows
to move the cursor
in the LCD display.
Press Options to
scroll through options
or the alphabet.
Press Shift+Options
to scroll backward
through options or
the alphabet.
Figure 8: Keypad
BioTek Instruments, Inc.
Keypad Description | 27
Select Menu Functions
The menu functions appear in the bottom (second) line of the display. To make a
selection, press the SoftKey on the keypad below the menu function.
Scroll Through Options
Certain functions, such as entering a program name or selecting a program type, offer
a set of options from which to make a selection. To view the different options, press the
Options key or the Shift + Options key combination. Press the ENTER key to select
the current option.
Enter Program Names
NAME:
-
Dispense 96
/
:
_
Each MicroFill™ program requires the entry of a unique name, using up to 16
alphanumeric characters. To enter a program name via the Dispenser keypad, press
Shift + key A-H, or scroll through the alphabet with the Options key for A-Z. Shift +
Options reverses direction. Use the right (forward) or left (back) arrows to move the
cursor within the display.
Press the ENTER key to store the completed program name and continue. If the
program name already exists, the dispenser software will require you to enter a unique
name. This protects dispenser programs from inadvertently being overwritten.
The NAME display offers four symbols that can be used in a program name: dash
(hyphen), forward slash, colon, and underscore. These symbols appear in the LCD as
SoftKey choices (see “Select Menu Functions” above). To include a symbol within a
program name, press its corresponding SoftKey.
Move Backward Through the Menus
To move to a previous menu, press the Previous Screen key.
Clear Entry
To delete an entry, press the CLEAR key.
Save Program
After defining a dispenser program, press the Main Menu key, and then press YES to
save the program.
Start/Stop Program
Press the START key to begin running a dispenser program. Press the STOP key to
stop running (abort) a program.
MicroFill Operator's Manual
28 | Chapter 3: Operation
Operation with the BioStack
If you have purchased BioTek’s BioStack Microplate Stacker to operate with the MicroFill,
refer to the instructions in your BioStack Operator’s Manual for setting up and
configuring the dispenser to operate with the BioStack.
You can run Dispense and Link programs on the MicroFill with or without the BioStack
attached. The Prime and Maintenance programs can only be run on the MicroFill without
the BioStack.
Main Menu
Following successful power-up of the MicroFill™, the Main Menu appears:
µFill Reagent Dispenser
RUN
DEFINE MAINT
UTIL
The following options are available through the Main Menu:
Under RUN:
SELECT PROGRAM TYPE:
DISP
PRIME
SOAK
LINK
Under DEFINE:
SELECT ACTION:
CREATE EDIT COPY
DELETE
Under MAINT:
SELECT MAINT PROGRAM:
<program name>
XX
Under UTIL:
SELECT UTILITY:
TESTS
SETUP
AUTOPRM
The RUN, DEFINE, and UTILITY options are described in this chapter.
See Chapter 4, Preventive Maintenance for information on the MAINTenance options.
BioTek Instruments, Inc.
Program Types | 29
Program Types
The following program types can be defined and run through the MicroFill™’s keypad:
•
Dispense: Define parameters such as Number of Strips, Flow Rate, and Dispense
Volume, for dispensing fluid to the microwells. See page 33.
•
Prime: Define routines for priming the fluid lines and manifold before dispensing
begins. See page 29.
•
Soak: Define a soak duration for inclusion in a Link program. See page 35.
•
Link: A series of Dispense and/or Soak programs “linked” together to run
sequentially and automatically. See page 36.
Prime Programs
Priming Considerations
Adequate priming is necessary to consistently achieve withinspecification accuracy and precision from the dispenser. We
recommend that you use a two-step technique to prime the
dispenser. This requires running a Prime program and then a
Dispense program. First, insert the priming plate and run a
Prime program that primes with 20 ml of the solution to be
dispensed. We recommend using a prime Rate of 5. Next, with
the priming plate still in place, run a Dispense program that
dispenses 20 μl per well to 24 columns of a 384-well microplate
at rate 2. This will pump any residual air out of the check valves.
The dispenser is now ready to run a dispense program. The
default programs New_Buffer_Prime and accuracy_test
may be used for this purpose, or you may create your own
programs.
When a Prime program is run, the priming plate must be in place in the microplate
adaptor. The priming plate has an internal partition that divides its interior into two
compartments (see Figure 9 on the next page).
•
When priming the instrument, the priming plate should be oriented with the
large compartment under the manifold.
•
When running a Maintenance program, the priming plate should be oriented
with the small compartment under the manifold.
MicroFill Operator's Manual
30 | Chapter 3: Operation
Internal partition
Small compartment – place under the manifold while
running a maintenance program
Large compartment – place under
the manifold while priming
Figure 9: Priming plate
The following sections discuss how to define, run, edit, copy, and delete Prime programs.
™ A prime trough drain kit is supplied (BioTek part number 7140006)
for the installed priming trough. With the drain kit in place, it is
possible to perform non-soak primes using the installed (fixed) priming
trough instead of the removable priming plate. The prime program,
TROUGH_PRIME, must be run for standalone primes using this trough.
It is possible to edit the parameters of this program.
BioTek Instruments, Inc.
Prime Programs | 31
Define Prime Program
To define a Prime program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
DEFINE > CREATE > PRIME > NAME > PRIME VOLUME > PRIME FLOW
RATE > SOAK AFTER PRIME? > SOAK DURATION > OK TO SAVE
PROGRAM?
Define Prime Program Options
Options
Actions
Name
Enter a unique name for the Prime program. See “Enter Program
Names” on page 27 for instructions.
Prime Volume
Specify the volume of the solution or rinse used to prime the
dispense tubes. The volume range is 1 to 200 ml.
Prime Flow Rate
Specify the rate at which the priming fluid is pumped into the
dispense tubes. The flow rate options range from 1 to 5, where
1 = Slowest, 5 = Fastest. The dispenser display shows the
corresponding µl/well/sec value for each option.
Soak After Prime
Yes/No moves the manifold to a lower position where the
dispense tubes can be soaked in the small compartment of the
priming plate. Useful for overnight maintenance and
decontamination programs. See Chapter 4.
Soak Duration
Specify the number of minutes and hours to soak. The range is
1 minute to 18 hours. This option is useful for Maintenance
programs; see Chapter 4.
OK to Save Program?
Select YES to save the program.
Run Prime Program
To run a Prime program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
RUN > PRIME > SELECT PRIME PROGRAM > INSERT PRIMING PLATE AND
PRESS <START> KEY > PRIME PROGRAM RUNNING. PRESS <STOP> KEY
TO QUIT > PRIME PROGRAM COMPLETE.* NEW REPEAT
*If NEW is pressed at the PRIME PROGRAM COMPLETE screen, a new program can be
selected and run. If REPEAT is pressed, the same program will be rerun.
MicroFill Operator's Manual
32 | Chapter 3: Operation
Edit Prime Program
To change a Prime program, follow the menu path shown here. Some of these items
are options (see the previous page), and some are prompts.
DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > Edit the
Program Parameters > MAIN MENU KEY > OK TO SAVE PROGRAM?
Copy Prime Program
To copy a Prime program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
DEFINE > COPY > PRIME > SELECT PRIME PROGRAM > NAME >
OK TO COPY?
Copy Prime Program Options
Options
Actions
Select Prime Program
Select the Prime program you wish to copy.
Name
Enter a unique name for the new Prime program.
OK to Copy?
Select YES to copy the program.
Delete Prime Program
To delete a Prime program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
DEFINE > DELETE > PRIME > SELECT PRIME PROGRAM >
OK TO DELETE?
Delete Prime Program Options
Options
Actions
Select Prime Program
Select the Prime program you wish to delete.
OK to Delete?
Select YES to delete the program.
BioTek Instruments, Inc.
Dispense Programs | 33
Dispense Programs
Before running a Dispense program, be sure to adequately
prime the dispenser. Failure to adequately prime the dispenser
may leave residual air in the fluid lines and check valves that
will be dispensed into the wells. See “Priming Considerations”
on page 29.
This section discusses how to define and run Dispense programs. Dispense programs
may also be edited, copied, and deleted. These actions are identical to those used for
editing, copying, and deleting Prime programs and are not repeated here (see page 32).
Define Dispense Program
To define a Dispense program, follow the menu path shown here. Some of these items
are options and some are prompts. The options are described below.
DEFINE > CREATE > DISP > NAME > PLATE TYPE > FIRST STRIP TO
DISPENSE > NUMBER OF STRIPS > DISPENSE VOLUME > DISPENSE FLOW
RATE > DISPENSE HEIGHT > PLATE CLEAR HEIGHT > HORIZONTAL DISP
POS > PRIME BEFORE START? > PRIME VOLUME > PRIME FLOW RATE >
OK TO SAVE PROGRAM?
Define Dispense Program Options
Options
Actions
Name
Enter a unique name for the Dispense program, using up to 16
alphanumeric characters. See “Enter Program Names” on page
27 for instructions.
Plate Type
(16-channel manifold only) Select a Plate Type of 96 if dispensing
into 8-well strips or 96-well plates, or 384 if dispensing into 384well plates.
First Strip to Dispense
Specify the first strip to dispense into. The range is 1 to 6 for 24well plates, 1 to 12 for 96-well plates, and 1 to 24 for 384-well
plates.
Number of Strips
Specify the number of strips to dispense into. The range is 1 to 6
for 24-well plates, 1 to 12 for 96-well plates, and 1 to 24 for 384well plates.
Dispense Volume
Specify the volume of solution to dispense per microwell, per
dispense cycle. The volume range is 10 to 3000 µl/well for 96well plates, 5 to 1500 µl/well for 384-well plates, and 20 to 6000
µl/well for 24-well plates.
MicroFill Operator's Manual
34 | Chapter 3: Operation
Dispense Flow Rate
Specify the rate at which the fluid is dispensed from the tubes.
The flow rate options range from 1 to 5, where 1 = Slowest,
5 = Fastest. The Dispenser display shows the corresponding
µl/well/sec value for each option. Not all dispense rates are valid
for all dispense volumes. Only valid dispense flow rates will be
displayed for the volume selected. Tables for valid flow rates per
volume are contained in Appendix C, Dispense Flow Rates. The
user must choose a valid flow rate to exit this screen.
Dispense Height
Dispense Height represents the height between the bottom of the
dispense tubes and the microplate adaptor surface on which the
plate rests, during a dispense.
The Dispense Height options range from 51 to 195. The
Dispenser display shows the corresponding measurement in
millimeters for each option.
51 is the closest to the adaptor surface, and 195 is the furthest
from this surface.
Plate Clear Height
The Plate Clear Height represents the height between the bottom
of the dispense tubes and the adaptor surface on which the plate
rests, after a dispense and whenever the carrier is moving.
The Plate Clear Height options range from 51 to 195. The
Dispenser display shows the corresponding measurement in
millimeters for each option.
Horizontal Disp Pos
Horizontal Dispense Position represents the position of the
dispense tube in relation to the center of the microwell.
The Horizontal Disp Pos options range from: -80 to 80.
The Dispenser display shows the corresponding measurement in
millimeters for each option.
A negative offset (-80 to -1) moves the dispense tubes away
from the front edge of the well. A positive offset (1 to 80) moves
the dispense tubes toward the front edge of the well. A setting of
0 indicates no offset (0.000 mm).
Prime Before Start?
Select YES to prime the dispense tubes before starting the
Dispense program. Note: Prime Before Start is intended to be a
small (2 ml or less) volume pre-prime. It occurs in the priming
trough that is integral to the carrier. The maximum volume of
this trough is 16 ml. Unless the optional prime trough drain kit is
installed, the trough must be emptied by the user when it
becomes full.
Prime Volume
Specify the volume of the buffer or rinse used to prime the
dispense tubes. The volume range is 5 to 1500 μl/well.
Prime Flow Rate
Specify the rate at which the priming fluid is pumped into the
dispense tubes. The flow rate options range from 1 to 5, where
1 = Slowest, 5 = Fastest. The dispenser display shows the
corresponding µl/well/sec value for each option.
Press Main Menu to end
Select YES to save the program.
BioTek Instruments, Inc.
Soak Programs | 35
Run Dispense Program
To run a Dispense program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
RUN > DISPENSE > SELECT DISPENSE PROGRAM > MANIFOLD NOT
PRIMED?* > FIRST STRIP TO DISPENSE > NUMBER OF STRIPS >
INSERT MICROPLATE AND PRESS <START> KEY > DISPENSE PROGRAM
RUNNING. PRESS <STOP> KEY TO QUIT > DISPENSE PROGRAM
COMPLETE.** NEW REPEAT
* MANIFOLD NOT PRIMED? appears only after instrument power-up or after a
Maintenance program has been run. Choose ABORT to prime or PROCEED to continue
without priming.
** If NEW is pressed at the DISPENSE PROGRAM COMPLETE screen, a new program
can be selected and run. If REPEAT is pressed, the same program will be rerun.
Run Dispense Program Options
Options
Actions
Dispense program
Select a Dispense program.
Prime?
Select a Prime program to run before starting the Dispense
program.
First Strip to Dispense
Specify the number of the microwell strip to start dispensing:
1 to 12 for 96-well plates
1 to 24 for 384-well plates
1 to 6 for 24-well plates
Number of Strips
Specify the total number of microwell strips to be dispensed:
1 to 12 for 96-well plates
1 to 24 for 384-well plates
1 to 6 for 24-well plates
Soak Programs
Soak programs are defined, edited, copied, and deleted in the same way as Prime and
Dispense programs. Soak programs are unique, however, because they cannot be run as
stand-alone programs. A Soak program can only be run as one of a series of programs in a
Link program.
•
A Soak program cannot be run independently; it must be selected as part of a Link
program (see next section).
•
A Soak program is essentially a delay built into a Link program.
MicroFill Operator's Manual
36 | Chapter 3: Operation
Link Programs
A Link program is a series of Dispense and/or Soak programs linked together, and is run
and deleted in the same way as other program types. A Link program cannot be copied or
edited, but its contents can be viewed by selecting:
DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > View the Program
Parameters
•
Predefined Dispense and Soak programs can be “linked” together and run
sequentially and automatically.
•
A Link program can contain up to 30 pre-defined programs, all of which must be
defined for the same manifold type and plate type (“96” or “384”, for 16-channel
manifold only).
•
Once defined, a Link program can be viewed, run, or deleted. It cannot be edited or
copied.
Define Link Program
To define a Link program, follow the menu path shown here. Some of these items are
options and some are prompts. The options are described below.
DEFINE > CREATE > LINK > NAME > PLATE TYPE > SELECT LINK 01
(02, 03, up to 30) TYPE > SELECT PROGRAM > OK TO SAVE
PROGRAM?
Define Link Program Options
Options
Actions
Name
Enter a unique name for the Link program. See “Enter Program
Names” on page 27 for instructions.
Plate Type
(Applies to 16-channel manifold only) Select a Plate Type of 96
to link programs defined for 8-well strips or 96-well plates, or
384 to link programs defined for 384-well plates.
Select Link (n) Type
Select either Dispense or Soak.
Select Program
Select each Dispense and/or Soak program to be included in the
Link program. Programs will run in the order in which they are
selected. Note: Only programs with the selected Plate Type will
be accessible for inclusion in the Link program.
Press Main Menu to end
Select YES to save the program.
BioTek Instruments, Inc.
Utility Menu Options | 37
Utility Menu Options
TESTS
Self-Check
Each time it is turned on, the MicroFill™ automatically runs a self-check to verify
system components. The self-check can also be run from the dispenser’s front panel
by pressing UTIL > TESTS > SLFCHK. The self-check includes verification of
the syringe, manifold, and carrier movement; and the software checksum.
If the test completes successfully, the Main Menu appears and the dispenser is
ready for use. If the test fails, the dispenser will “chirp” and display an error code.
Press the STOP key and then look up the error code in Appendix A, Error Codes.
Configuration Information (CHKSUM)
The MicroFill stores the basecode software part number, version number, and
checksum value. This information is important if you need to contact BioTek’s
Technical Assistance Center. To view this information, start at the Main Menu and
select UTIL > TESTS > CHKSUM.
SETUP
Language
Your MicroFill’s display may be configured for one of several languages, including
English, French, German, Italian, and Spanish. To change the language, follow the
menu path shown below:
UTIL > SETUP > LANGUAGE > ENGLISH, FRENCH, GERMAN, ITALIAN,
or SPANISH > MAIN MENU KEY
Manifold
Your autoclavable MicroFill may be configured for one of three manifolds: the
standard 16-channel manifold, the “8-96” manifold, or the “8-24” manifold.
When physically changing manifolds, you must specify the manifold in use in the
software. To change the manifold setting, follow the menu path shown below:
UTIL > SETUP > MANIFOLD > 16ch, 8-96ch, or 8-24ch >
MAIN MENU KEY
MicroFill Operator's Manual
38 | Chapter 3: Operation
BIOSTACK
UTIL > SETUP > BIOSTACK > CONF, ALIGN, or VERIFY
The BIOSTACK UTILITIES screen will appear when BIOSTACK is selected. The
MicroFill™ can then be configured for proper operation with the BioStack by
selecting the CONF (Configuration), ALIGN (Alignment), and VERIFY
(Verification) utilities. Refer to your BioStack Operator’s Manual for detailed
instructions.
AUTOPRIME
Frequent priming keeps the dispenser’s manifold tubes moist and helps to prevent
them from clogging. The MicroFill provides an AUTOPRIME self-priming program,
which you can enable, disable, or edit (see below). If enabled, the program will run
automatically after the dispenser is idle for the specified period of time. See
“AutoPrime” in Chapter 4 for more information.
•
To enable the AutoPrime feature, follow the menu path shown below and
enter an autoprime interval from 1 to 600 minutes.
UTIL > AUTOPRIME > ENABLE AUTOPRIME? > YES
•
To disable the AutoPrime feature, follow the menu path shown below:
UTIL > AUTOPRIME > ENABLE AUTOPRIME? > NO
•
To interrupt AutoPrime, press the STOP key.
Editing the AutoPrime Program
The AutoPrime Prime program can be edited to change the length of the soak
period, eliminate the soak period, or to change the interval between AutoPrime
cycles. To edit this program, follow the menu path shown below.
DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > Edit the
Program Parameters (see below) > MAIN MENU KEY > OK TO SAVE
PROGRAM?
AutoPrime Program Options
Options
Actions
Prime Volume
Specify the volume of the buffer or rinse used to prime the
manifold. The volume range is 1 to 200 ml. Do not specify
more volume than can be safely contained in the priming
plate during idle periods.
Prime Flow Rate
Specify the rate at which the priming fluid is pumped into
the dispense tubes. The flow rate options range from 1 to
5, where 1 = Slowest, 5 = Fastest. The dispenser display
shows the corresponding µl/well/sec value for each option.
BioTek Instruments, Inc.
Utility Menu Options | 39
Options
Actions
Soak after Prime?
Select YES to soak the manifold tubes after they have
been primed. The priming solution is dispensed to the
small compartment of the priming plate. The manifold is
lowered into the plate and the tubes are submerged in the
priming fluid. After soaking, the manifold is raised to its
home position.
Soak Duration
Soak Duration represents the time to allow the dispense
tubes to be soaked in priming solution, rinse, or
disinfectant. The range is from 1 minute to 18 hours.
AutoRinse
By connecting a rinse bottle to the dispenser, the AutoPrime feature can be
used to automatically rinse the dispenser.
MicroFill Operator's Manual
40 | Chapter 3: Operation
BioTek Instruments, Inc.
Chapter 4
Preventive Maintenance
This chapter provides step-by-step instructions for maintaining the
MicroFill™ Microplate Dispenser in top condition, to ensure that it
continues to perform to specification.
Overview ............................................................................
Recommended Maintenance Schedule .....................................
Tools and Supplies ...............................................................
Maintenance and Prime Programs ...........................................
Daily Maintenance ................................................................
Overnight Maintenance .........................................................
Monthly Maintenance ............................................................
Long-Term Storage Maintenance ............................................
Storing the MicroFill Dispenser ...............................................
Decontamination ..................................................................
Daily/Overnight Maintenance Log
Monthly Maintenance Log
42 43 43 44 45 49 50 57 57 58 42 | Chapter 4: Preventive Maintenance
Overview
Preventive Maintenance (PM) represents a set of procedures that should be performed
regularly to maintain equipment in top condition. For example, during normal operation,
salt crystals or protein can build up and clog the valves and tubes. Daily and periodic
maintenance reduces this problem, maximizes running time, and extends the life of the
dispenser.
A routine maintenance schedule consists of:
•
Daily Maintenance
•
Overnight Maintenance
•
Monthly Maintenance
•
Long-Term Storage Maintenance
•
Decontamination
The MicroFill’s on-board software contains some predefined Maintenance programs,
including:
•
M_Day_Rinse
•
M_Rinse_And_Soak
•
M_Overnight_Loop
•
M_Long_Shutdown
•
M_Decontaminate
These programs are accessible from the dispenser’s Main Menu, under the MAINT option.
The following chart lists preventive maintenance tasks, the frequency with which each task
should be performed, the predefined program that should be run, and where to find more
information. Maintenance logs are provided at the end of this chapter.
BioTek Instruments, Inc.
Recommended Maintenance Schedule | 43
Recommended Maintenance Schedule
Purpose/Frequency
Tasks
Page
Daily
Monthly/
As Needed
Before Storage/
Shipment
Daily maintenance
Program: M_Rinse_And_Soak
45
9
Remove protein residuals and
fungi growth (if necessary)
46
9
AutoPrime
48
9
Overnight maintenance
Program: M_Overnight_Loop
49
9
Clean bottles
50
9
9
Clean manifold channels and
chambers
50
9
9
Clean dispense tubes with
stylus
50
9
9
Clean plate adaptor, carrier,
and transport rail
51
9
9
Clean priming plate and trough
51
9
9
Clean tubing and check valves
52
9
9
Remove liquid from fluid path
Program: M_Long_Shutdown
57
Decontaminate the instrument
Program: M_Decontaminate
58
9
9
9
Tools and Supplies
•
Stylus
•
Dish soap or other mild cleaner
•
¼” straight screwdriver
•
Deionized or distilled water
•
Lint-free disposable towels
•
(2) Check valves (PN 68080)
•
70% isopropyl alcohol
•
(2) Manifold plugs (PN 45090)
MicroFill Operator's Manual
9
44 | Chapter 4: Preventive Maintenance
Maintenance and Prime Programs
Each predefined Maintenance program runs a predefined Prime program. The charts
below show the default prime program parameters. These parameters can be modified.
Maintenance Program
M_Day_Rinse
Prime Program:
P_Day_Rinse
Prime Volume:
40 ml
Flow Rate:
5
Soak After Prime?
No
Maintenance Program
M_Rinse_And_Soak
Prime Program:
P_Rinse_And_Soak
Prime Volume:
40 ml
Flow Rate:
5
Soak After Prime?
Yes
Soak Duration:
5 minutes
Maintenance Program
M_Overnight_Loop
Prime Program:
P_Rinse_Loop
Prime Volume:
10 ml
Flow Rate:
5
Soak After Prime?
Yes
Soak Duration:
12 hours
BioTek Instruments, Inc.
Daily Maintenance | 45
Daily Maintenance
For all Daily Maintenance tasks, ensure that the:
•
supply bottle contains at least 400 ml of rinse solution
•
waste bottle is empty
•
priming plate is properly inserted in the microplate adaptor.
Rinse and Soak
Daily Maintenance involves flushing the dispenser with an appropriate reagent at the
beginning of the day (BioTek recommends using deionized water in the morning).
The M_Rinse_And_Soak program is recommended for the morning. This should be
performed on the same day the microplates are dispensed. This procedure
helps prevent the dispense tubes from clogging between uses.
The default parameters for the M_Rinse_And_Soak program are listed below.
Maintenance Program
M_Rinse_And_Soak
Prime Program:
P_Rinse_And_Soak
Prime Volume:
40 ml
Flow Rate:
5
Soak After Prime?
Yes
Soak Duration:
5 minutes
To run the M_Rinse_And_Soak program, follow the menu path shown below.
MAINT > Press Options until M_Rinse_And_Soak appears >
CONNECT RINSE AND PRESS <START> KEY > MAINTENANCE RUNNING
PRESS <STOP> TO QUIT
MicroFill Operator's Manual
46 | Chapter 4: Preventive Maintenance
Removing Protein Residuals and Fungi Growth
Important! Solutions containing proteins, such as bovine
serum albumin (BSA), will compromise the dispenser’s
performance over time unless a strict maintenance regime
is adhered to.
Do not use isopropyl alcohol to flush out BSA.
BioTek recommends performing the following additional Maintenance procedures to
thoroughly flush out protein particles and other contaminants from the dispenser’s
fluid path, if necessary:
Daily
Using an Enzyme-Active Detergent:
1.
Mix an enzyme-active detergent according to the manufacturer’s directions
to fill a two-liter supply bottle (or a one-liter bottle, for an autoclavable
MicroFill™).
2.
Run the M_Decontaminate Maintenance program. When the program
pauses and displays CONNECT RINSE PRESS <START> KEY, leave the
detergent bottle connected and press the Start key to continue. Repeat this
sequence until the bottle is empty.
3.
Connect a bottle full of deionized water and run the P_Day_Rinse Prime
program three times to flush the system.
Using Ethanol:
At the end of the day:
1.
Prepare a two-liter solution of 10% ethanol and deionized water (or a oneliter solution for an autoclavable MicroFill™). Do not use isopropyl
alcohol.
2.
Run the M_Decontaminate Maintenance program.
3.
Connect a bottle full of deionized water and flush the system by running
either the P_Day_Rinse Prime program (three times), or the
M_Overnight_Loop Maintenance program.
BioTek Instruments, Inc.
Daily Maintenance | 47
Using Deionized Water:
If the dispenser will be idle between plates for longer than 45 minutes, flush the
proteins from the system:
1.
Fill a supply bottle with deionized water. Connect the bottle to the
dispenser.
2.
Run the P_Day_Rinse Prime program.
3.
Enable AUTOPRIME for 60-minute intervals.
At the end of the day:
1.
Fill a supply bottle with deionized water. Connect the bottle to the
dispenser.
2.
Run the P_Day_Rinse Prime program three times.
3.
Run the M_Overnight_Loop Maintenance program.
Weekly/Monthly
1.
Flush the system with 0.1 – 0.5 N NaOH, followed by neutralization with an
equivalent normality (0.1 – 0.5 N) of HCl.
2.
Rinse well with deionized water to remove the HCl.
3.
Run the M_Overnight_Loop Maintenance program with deionized
water, or run the P_Day_Rinse Prime program three times with deionized
water if you plan to use the dispenser immediately.
MicroFill Operator's Manual
48 | Chapter 4: Preventive Maintenance
AUTOPRIME
BioTek recommends enabling the AutoPrime feature to automatically prime (and
optionally soak) the dispenser after it has been idle for a user-specified amount of time.
The volume, flow rate, and soaking parameters specified in the AUTOPRIME Prime
program are used. AutoPrime is particularly useful if the dispenser is used
intermittently throughout the day, to keep the fluid lines and manifold in a wetted
condition. If you prefer not to enable the AutoPrime feature, BioTek recommends
running the M_Rinse_And_Soak program periodically during the day.
™ If AutoPrime is enabled, the priming plate must be left in the
microplate adaptor when the user finishes dispensing a plate. The
partition dividing the trough must be placed toward the rear of the
instrument.
The AutoPrime feature is disabled by default. To enable it:
1.
Select UTIL > AUTOPRIME > YES.
2.
Enter the interval from 10 to 600 minutes, to indicate how often you want
the AutoPrime Prime program to run.
3.
Press ENTER and then return to the Main Menu.
AutoPrime is now enabled and priming will occur each time the dispenser has been
idle for <interval> minutes.
•
To disable the AutoPrime feature, select UTIL > AUTOPRIME > NO.
•
To halt the AUTOPRIME program when it is running, press the STOP key.
•
To view or edit the AUTOPRIME program parameters, select DEFINE >
EDIT > PRIME > AUTOPRIME and then press the Options key to scroll
through the parameters you wish to view or edit. The table below shows
the AUTOPRIME program parameters and ranges.
Parameter
Default
Selectable Range
Interval
10 minutes
10 to 600 minutes
Prime Volume
10 ml
1 to 999 ml
Prime Flow Rate
5
1 to 5
Soak After Prime?
Yes
Yes/No
Soak Duration
11 hours, 50 minutes
1 minute to 18 hours
™ See “Prime Programs” in Chapter 3 for more information on Prime
programs and their parameters.
BioTek Instruments, Inc.
Overnight Maintenance | 49
Overnight Maintenance
When the dispenser is left overnight or for the weekend, we recommend you run the
M_Overnight_Loop Maintenance program. Overnight/multi-day maintenance involves
flushing all dispense solution out of the instrument, and then periodically rinsing and
soaking the tubes to keep them moist.
•
Connect a supply bottle containing deionized water and run M_Day_Rinse
to flush any solution out of the dispenser. Run the M_Overnight_Loop
program to prime and soak the tubes.
Important! Keep the manifold in a wetted condition to
ensure proper operation. BioTek recommends changing the
Soak Duration to 18 hours for overnights and weekends
when the dispenser is not in use. This will decrease flow
problems and is a more trouble-free way to operate the
dispenser.
•
The M_Overnight_Loop program requires the dispenser to be kept plugged
in and turned on. As an alternative, run the M_Rinse_And_Soak
Maintenance program and turn off the instrument after the soak begins. This
leaves the tubes soaking in the priming plate until the instrument is turned on
again.
•
The M_Overnight_Loop program will run continuously until the STOP key
is pressed. After pressing the STOP key, select YES to home all axes.
To run the M_Overnight_Loop program, follow the menu path shown below.
MAINT > press Options until M_Overnight_Loop appears >
CONNECT RINSE AND PRESS <START> KEY > MAINTENANCE RUNNING
PRESS <STOP> TO QUIT
MicroFill Operator's Manual
50 | Chapter 4: Preventive Maintenance
Monthly Maintenance
Clean the Bottles and Tubes
•
Clean the dispense and rinse bottles and supply tubes with deionized water before
the first use, before each refill, and if they have been idle for any length of time.
•
Accumulated algae, fungi, or mold may require decontamination; see page 58.
Clean the Manifold
Regular rinsing helps to keep the manifold clean and the dispense tubing clear, and
will increase the life of the tubing. Follow the Decontamination procedure starting on
page 58 to disinfect the manifold and tubing.
If you suspect a particular problem is related to the manifold (for example, clogged
tubes can result in uneven dispensing), you should perform a thorough cleaning of the
manifold. To clean the manifold:
1.
Turn off and unplug the instrument.
2.
Remove the mist shield. See Figure 7 in Chapter 2.
3.
Disconnect the tubing from the back of the manifold. See Figure 5 in
Chapter 2.
4.
Remove the thumbscrews and the manifold. Using a lint-free disposable
towel, thoroughly clean the outside of the dispense tubes.
5.
Run hot water through the inlet fitting. Check to see if water comes out of
all of the dispense tubes. If not, soak the manifold in hot soapy water and
repeat. Clean the tubes with the stylus if necessary (see below).
Clean (“Stylize”) the Dispense Tubes
™ Note: The autoclavable manifold has removable dispense tubes. We
do not recommend routinely removing these tubes. In the case of a
particularly difficult problem with any one channel, however, a tube
may be removed and cleaned individually, or replaced.
The manifold dispense tubes do not need to be “stylized” unless there is a problem.
Periodic rinsing helps prevent the need for stylizing. If the previous steps are not
completely successful, try the following:
1.
Remove the two gray plugs from the ends of the manifold.
2.
Tip the manifold on end and flush hot water through this open channel.
3.
Using the supplied stylus (PN 2872304), clean the insides of all of the
dispense tubes.
BioTek Instruments, Inc.
Monthly Maintenance | 51
4.
Tip the manifold on end and flush hot water through the open channel.
5.
Rinse the manifold with deionized or distilled water. Reinsert the gray
plugs into the ends of the manifold. Check to see if water comes out of all of
the dispense tubes.
6.
Remount the manifold and replace the tubing. See Figures 1 and 5 in
Chapter 2.
7.
Replace the mist shield.
8.
Run a Prime program using 40 ml of deionized water.
9.
Verify dispense performance visually, or by performing the appropriate
Dispense Precision and Accuracy Tests in Chapter 5.
Clean the Plate Carrier
™ Note: Each plate carrier is specifically aligned to each MicroFill™. Do
not adjust the carrier’s nylon screws or interchange the carrier with
another.
If liquid has overflowed onto the plate carrier, some crystal buildup may occur and
prevent the plate adaptor from seating correctly onto the carrier.
1.
Remove the carrier:
•
Move it to its full “out” position (turn the dispenser on, wait for the
system test to complete, and then turn the dispenser off). Make sure the
manifold and tubes are over the priming trough (“home” position).
•
Carefully lift the carrier front off the rail, avoiding contact with the
manifold tubes.
•
Carefully lift the carrier rear off the rail, avoiding contact with the
manifold tubes.
2.
Clean the carrier using soap and water or 70% isopropyl alcohol.
3.
Replace the carrier:
•
Hold the microplate carrier over the rail so that the trough is to the rear
of the dispenser.
•
Line up the pin on the underside of the carrier with the slot on the
carrier transport.
•
Snap the two carrier clips onto the rail. The pin must sit in the slot.
MicroFill Operator's Manual
52 | Chapter 4: Preventive Maintenance
Clean the Plate Carrier Transport Rail
Important! Do not allow the alcohol or water to seep
in between the rail and the plate carrier transport
mechanism that rides on the rail. Follow the instructions
carefully to prevent this.
If liquid has overflowed onto the stainless steel plate carrier transport rail, some crystal
buildup may occur, preventing the carrier from moving properly.
To clean the plate carrier transport rail:
1.
Turn off the instrument.
2.
Carefully lift the microplate carrier off of its transport mechanism.
3.
Moisten (do not soak) a cloth with alcohol and wipe the back half of the rail.
After several minutes, wipe the rail with a cloth moistened (not soaked)
with deionized water. Wipe with a dry cloth.
4.
When the back half of the rail is completely dry, slide the plate carrier
transport mechanism to the back of the rail.
5.
Repeat step 3 for the front half of the rail.
6.
When the front half of the rail is completely dry, replace the microplate
carrier and turn on the instrument.
Clean/Replace the Check Valves
™ Note: The check valves for autoclavable dispensers do not twist
open.
If the check valves leak or become stuck, you can either clean or replace them.
To order replacment check valves, contact BioTek Customer Service.
To clean a check valve:
1.
Pull the tubing off the check valve and pull the valve off the syringe pump
port. See Figure 3 in Chapter 2.
2.
Insert the stylus into the feed end of the valve to hold it open (observe
arrow on valve indicating flow direction).
3.
Flush with hot water. If necessary, for the non-autoclavable model, the
check valve body twists open to allow disassembly and cleaning of internal
components. Note proper orientation for reassembly.
4.
Replace the valve and the tubing.
BioTek Instruments, Inc.
Monthly Maintenance | 53
After replacing a check valve, we recommend that
you recalibrate the backlash (see below) to
ensure optimum accuracy performance.
Calibrating the Backlash
Equipment Required
•
A minimum 3-place precision balance (all data from 0 to 99 grams)
•
Supply bottle
•
Priming plate
•
Supply Tubing Sets:
For AF1000 Dispensers (PN 7140505)
For AF1000A Autoclavable Dispensers, use PN 7140510, or use the Organic
Solvent Tubing Set, PN 7140514 .
Setup
1.
Connect the dispenser to a supply bottle that is half-filled with deionized
water. The deionized water container must be at the same level as
the dispenser. The liquid level should be 3” (102 mm) from the bench top.
While calibrating and testing, maintain the liquid level in the supply bottle
at half the 3-inch column height, but start with more fluid to allow for
priming.
2.
Attach the fitting of the long tube to the supply bottle. Push the opposite
end of this tubing onto the bottom port of the syringe pump (see Figure 3
in Chapter 2). The arrow of the check valve must point toward the
syringe pump.
™ Note: For AF1000 dispensers, the check valve will be closer to the
syringe pump than to the manifold. For AF1000A dispensers, the
check valve will be closer to the manifold.
3.
Push one end of the short supply tube onto the top port of the syringe
pump. Push the other end over the barbed fitting on the manifold.
Select the Manifold (AF1000A Dispensers Only)
Before setting the backlash, it is necessary to select the manifold being tested.
Follow the menu path shown below:
UTIL > SETUP > MAN > 16ch or 8ch-96 or 8ch-24 >
MAIN MENU KEY
MicroFill Operator's Manual
54 | Chapter 4: Preventive Maintenance
Create a Dispense Program
The backlash is adjusted for a 20 μl dispense volume. Create a program that
dispenses 20 μl per well at dispense rate 2. Follow the menu path shown below:
DEFINE > CREATE > DISP > key in a program name > set the
DISPENSE VOLUME to 0020 and the FLOW RATE to 2 > MAIN MENU
KEY > YES
Prime the Plate
1.
Place the microplate adaptor into the carrier, and place the priming plate into
the adaptor.
2.
Follow the two menu paths shown below:
RUN > PRIME > OPTIONS key until New_Buffer_Prime is
displayed > ENTER KEY to select the program > ENTER KEY
to start the program > When the prime is complete, press
the REPEAT soft key and prime again > MAIN MENU KEY
RUN > DISP > OPTIONS key until the newly created
Dispense program is displayed > ENTER KEY to start this
program > ENTER KEY to select the defaults for the first
strip to dispense and the last strip to dispense > ENTER
KEY again to start the program
The MicroFill™ will dispense 20 μl per tube into the priming trough.
3.
Empty the Priming Plate as needed.
Calibrate the Backlash
1.
Empty/dry the priming plate, place it on a three-place balance, and then tare
the balance.
2.
Place the priming plate onto the adaptor and run the 20 μl Dispense program
created above.
3.
When the program is complete, carefully remove and reweigh the plate, to
determine the Actual Weight.
The Expected Weight is:
•
7.680 g for 16-channel manifolds
•
1.920 g for 8-channel manifolds (AF1000A versions only)
BioTek Instruments, Inc.
Monthly Maintenance | 55
4.
Calculate the volume (weight) error and the backlash adjustment that needs to
be made.
•
Subtract the Actual Weight from the Expected Weight and divide by the
number of columns dispensed.
•
Divide the result by 0.0033 for AF1000A instruments, or 0.0031 for AF1000
instruments, and round to the nearest whole number.
Example: 7.680 - 7.550 = 0.130, 0.130/24 = 0.00542, 0.00542/0.0033 = 1.64
rounded to 2. The backlash setting needs to be adjusted by 2.
Example: 1.920 - 2.010 = -0.09, -0.09/12 = - 0.0075, -0.0075/0.0033 = -2.27
rounded to -2. The backlash setting needs to be adjusted by -2.
5.
Adjust the backlash as necessary:
UTIL Î TESTS Î press the SHIFT key while simultaneously
pressing the HIDDEN KEY #2 (space between the Main Menu
and Previous Screen keys) Î SYR Î BKLSH Î enter the
new number Î ENTER KEY Î MAIN MENU KEY
6.
Repeat steps 1 through 5 until the volume dispensed is within one backlash
unit of being exact:
•
AF1000 dispenser: ± 0.112 ml
•
AF1000A dispenser w/16-channel manifold: ± 0.119 ml
•
AF1000A dispenser w/8-channel manifold: ± 0.059 ml
MicroFill Operator's Manual
56 | Chapter 4: Preventive Maintenance
Autoclaving the Syringe Head
This section applies to autoclavable versions of the MicroFill™ only.
™ Note: Refer to Figure 2 in Chapter 2 when performing steps 1-3.
1.
Use the supplied 3/32” (2.39 mm) hex wrench to remove the two mounting
screws that hold the syringe head to the rear of the instrument.
2.
Pull the syringe head straight back and off of the piston.
3.
Use the hex wrench to loosen the setscrew and then remove the piston.
Important! The piston and syringe head must be
autoclaved separately from one another (see Figure 10
below). Ensure that the piston is removed from the
syringe head before autoclaving. If a mistake is made
and the piston is set in the syringe head through the
autoclave cycle, remove the piston from the syringe head
and run the piston (or both piston and syringe head)
through the autoclave again.
4.
Autoclave at 134°C and 216 kPa for 3 minutes, or 121°C and 115 kPa for 30
minutes. The manifold, tubing, check valves, and supply bottles may also be
sterilized in the autoclave.
5.
Replace the components as described in Chapter 2.
Figure 10: Autoclave the piston (left) and syringe head (right) separately
BioTek Instruments, Inc.
Long-Term Storage Maintenance | 57
Long-Term Storage Maintenance
Before leaving the MicroFill™ unused for a long period of time, run the
M_Long_Shutdown Maintenance program. This program performs three steps:
•
Flushes and soaks the supply tubing and manifold with disinfectant
•
Flushes the system with rinse
•
Purges the system of fluid
Prepare one supply bottle with at least 100 ml of disinfectant (70% isopropanol) and one
with at least 100 ml of rinse (deionized water). You also need one empty supply bottle (this
is the “air” bottle).
To run the M_Long_Shutdown program, follow the menu path shown below.
MAINT > press the Option key until M_Long_Shutdown appears >
CONNECT DISINFECTANT AND PRESS <START> KEY > MAINTENANCE
RUNNING PRESS <STOP> TO QUIT
™ Note: While this program is running, you will need to periodically
check the display and follow the instructions for changing the supply
bottles.
Storing the MicroFill Dispenser
1.
Perform the Long-Term Storage Maintenance as described above.
2.
Disconnect the dispenser from its power source.
3.
Store the dispenser in a dust- and particle-free environment as described in
Chapter 1.
4.
Protect the dispenser from temperature extremes that can cause condensation within
the unit and from corrosive fumes and vapors.
MicroFill Operator's Manual
58 | Chapter 4: Preventive Maintenance
Decontamination
Purpose
Any laboratory instrument that has been used for research or clinical analysis is
considered a biohazard and requires decontamination prior to handling.
Decontamination minimizes the risk to all who come into contact with the instrument
during shipping, handling, and servicing. Decontamination is required by the U.S.
Department of Transportation regulations.
Persons performing the decontamination process must be familiar with the basic setup
and operation of the instrument.
Important! BioTek Instruments, Inc. recommends the use
of the following decontamination solutions and methods
based on our knowledge of the instrument and
recommendations of the Centers for Disease Control and
Prevention (CDC). Neither BioTek nor the CDC assumes any
liability for the adequacy of these solutions and methods.
Each laboratory must ensure that decontamination
procedures are adequate for the Biohazard(s) they handle.
Wear prophylactic gloves when handling contaminated
instruments. Gloved hands should be considered
contaminated at all times; keep gloved hands away from
eyes, mouth, nose, and ears. Eating and drinking while
decontaminating instruments is not advised.
Mucous membranes are considered prime entry routes for
infectious agents. Wear eye protection and a surgical mask
when there is a possibility of aerosol contamination. Intact
skin is generally considered an effective barrier against
infectious organisms; however, small abrasions and cuts may
not always be visible. Wear protective gloves when
performing the decontamination procedure.
BioTek Instruments, Inc.
Decontamination | 59
Tools and Supplies
•
0.5% sodium hypochlorite (NaClO, or bleach)
•
Surgical mask
•
70% isopropyl alcohol (as an alternative to bleach)
•
Protective gloves
•
Deionized or distilled water
•
Lab coat
•
Priming plate
•
Biohazard trash bags
•
Safety glasses
•
Clean cotton cloths
Decontamination Procedure
Warning! Internal Voltage. Always turn off and unplug
the instrument for all decontamination and cleaning
operations.
The bleach solution is caustic; wear gloves and eye protection
when handling this solution.
Do not immerse the instrument, spray it with liquid, or use a
“wet” cloth. Do not allow the cleaning solution to run into the
interior of the instrument. If this happens, contact the BioTek
Service Department.
Do not soak the keypad. This will cause damage. Wipe the
keypad with a damp cloth.
1.
Turn off and unplug the instrument.
2.
Prepare an aqueous solution of 0.5% sodium hypochlorite (bleach). As an
alternative, 70% isopropyl alcohol may be used if the effects of bleach are a
concern.
™ Note: Be sure to check the % NaClO of the bleach you are using; this
information is printed on the side of the bottle. Commercial bleach is
typically 10% NaClO; if this is the case, use a 1:20 dilution. Household
bleach is typically 5% NaClO; if this is the case use a 1:10 dilution.
™ Isopropyl alcohol is not recommended for removing proteins (such
as bovine serum albumin).
3.
Remove the dispenser’s mist shield.
MicroFill Operator's Manual
60 | Chapter 4: Preventive Maintenance
4.
Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth.
5.
Wipe the inside of the mist shield, the plate carrier, microplate adaptor,
priming trough, priming plate, and the top surface of the base.
6.
Wait 20 minutes. Moisten a cloth with DI or distilled water and wipe all
surfaces of the instrument that have been cleaned with the bleach solution or
alcohol.
7.
Use a clean, dry cloth to dry all wet surfaces.
8.
Place a microplate adaptor into the carrier, and place the priming plate into the
adaptor. Ensure that the partition dividing the trough is placed toward the rear
of the instrument.
9.
Run the M_Decontaminate program to decontaminate the internal tubing
and manifold parts. Follow the menu path shown below:
MAINT > M_Decontaminate > CONNECT DISINFECTANT AND PRESS
<START> KEY Î RUNNING: DECONTAMINATION/DISINFECTING Î
CONNECT RINSE BOTTLE AND PRESS <START> KEY Î RUNNING:
DECONTAMINATION/RINSING
™ Note: While this program is running, you will need to periodically
check the display and follow the instructions for changing the supply
bottles.
10. Reassemble the dispenser as necessary.
11. Discard the used gloves and cloths using a Biohazard trash bag and an
approved Biohazard container.
BioTek Instruments, Inc.
© 2008, BioTek Instruments, Inc.
Operator’s
Initials
Other:
Overnight Loop
Overnight Maintenance (9 box when task is completed)
Operator’s
Initials
Other:
AutoPrime
Remove protein
residuals
Rinse and Soak
Daily Maintenance (9 box when task is completed)
DAY:
Month: _______________
MicroFill™ Serial Number: ______________
Daily/Overnight Maintenance Log
© 2008, BioTek Instruments, Inc.
Operator’s Initials
Other:
Decontaminate
Calibrate backlash
Clean tubing and check
valves
Clean priming plate and
trough
Clean plate carrier,
adaptor, and transport rail
Clean dispense tubes
with stylus
Clean manifold channels
and chambers
Clean bottles and tubes
Month/Year:
Check (9) box when task is completed
MicroFill™ Serial Number: ______________
Monthly Maintenance Log
Chapter 5
Instrument Qualification
Testing is conducted on every MicroFill™ before it leaves BioTek, to
verify dispense accuracy and precision.
™ Note: Disable the AutoPrime feature before conducting the tests.
IQ/OQ/PQ ...........................................................................
Recommended Qualification Schedule .....................................
System Self-Check and Checksum Test ...................................
System Self-Check ...........................................................
Checksum Test ................................................................
Dispense Precision and Accuracy Tests ....................................
Overview ........................................................................
Specifications ..................................................................
Materials ........................................................................
Test Solutions .................................................................
Dispense Precision & Accuracy Test: 16-Channel Manifold ......
Dispense Precision & Accuracy Test: “8-96” Manifold ............
Dispense Precision & Accuracy Test: “8-24” Manifold ............
Documenting Test Results .................................................
Liquid Test Worksheets
Performance Verification Logs
62 63 64 64 64 64 64 65 66 67 68 70 72 74 62 | Chapter 5: Instrument Qualification
IQ/OQ/PQ
Installation Qualification (IQ) confirms that the dispenser and its components have
been supplied as ordered and ensures that they are assembled and configured properly for
your lab environment.
•
The recommended IQ procedure consists of setting up the instrument as
described in Chapter 2, Installation and then performing the System SelfCheck and the Checksum Test.
•
The IQ procedure should be performed initially (before the dispenser is used
for the first time).
•
The successful completion of the IQ procedure verifies that the instrument is
installed correctly. The Operational Qualification procedure (see below) should
be performed immediately following the successful IQ.
Operational Qualification (OQ) confirms that the dispenser operates according to
specification initially and over time.
•
The recommended OQ procedure consists of performing the System SelfCheck, the Checksum Test, and the appropriate Dispense Precision and
Accuracy Tests.
•
The OQ procedure should be performed initially (before first use) and then
routinely; the recommended interval is annually. It should also be performed
after any major repair or upgrade to the hardware or software.
•
Although out-of-tolerance failures will be detected by the OQ tests, results
should be compared with those from the monthly Performance Qualification
tests and previous OQ tests to monitor for trends.
•
The successful completion of the OQ procedure, in combination with results
that are comparable to previous PQ and OQ tests, confirms that the dispenser is
performing consistently over time.
BioTek Instruments, Inc.
Recommended Qualification Schedule |
63
Performance Qualification (PQ) confirms that the dispenser consistently meets the
requirements of the tests performed at your laboratory.
•
The recommended PQ procedure consists of performing the System Self-Check,
the Checksum Test, and the Dispense Precision and Accuracy Test.
•
Your facility’s operating policies may also require that you routinely perform
an actual assay, to confirm that the dispenser will consistently give adequate
results for the assays to be run with it.
•
These tests should be performed routinely; the recommended interval is
monthly. This frequency may be adjusted depending on the trends observed
over time.
•
The successful completion of the PQ procedure confirms that the dispenser is
performing consistently under normal operating conditions.
Recommended Qualification Schedule
Task/Test
IQ
OQ
PQ
Initially
Initially &
Annually
Monthly
Installation, Setup, & Configuration
9
System Self-Check
9
9
9
Checksum Test
9
9
9
9
9
Dispense Precision and Accuracy Test
MicroFill Operator's Manual
64 | Chapter 5: Instrument Qualification
System Self-Check and Checksum Test
System Self-Check
Each time the MicroFill™ is turned on, the System Self-Check is automatically
performed to ensure proper instrument operation.
If the self-check passes, the Main Menu will appear and the dispenser is ready for use.
If there is a failure, the dispenser will “chirp” and display an error code. Press the Stop
key and then look up the error code in Appendix A. If the dispenser fails the selfcheck, it will not perform a program operation until the error condition is cleared.
The System Self-Check can also be performed manually, by selecting
UTIL > TESTS > SLFCHK.
Checksum Test
If the Checksum Test is performed, the initial display will show the on-board
(basecode) software part number, version number, and checksum. The second display
shows the configuration software information. The Main Menu reappears at the
conclusion of the test.
To run the Checksum Test, start at the Main Menu and select
UTIL > TESTS > CHKSUM.
Dispense Precision and Accuracy Tests
Overview
™ Note: Disable the AutoPrime feature before conducting these tests.
Dispense precision is a measure of the variability of volumes dispensed from tube to tube
across the manifold. The optical density of the solution in a well is proportional to the total
volume of the solution in the well. If the % Coefficient of Variation (%CV) is calculated, the
result is a measure of the uniformity of the distribution of dispensed volumes across the
manifold. It is the ratio, expressed in percent, of the standard deviation of the distribution
of fluid volumes in the wells to the mean value of volume per well. The uniformity of
distribution across the manifold improves as the %CV is lowered.
Dispense accuracy is a measure of the average volume dispensed per well. It is
independent of precision. The volume per well may vary greatly over a plate, yet the
accuracy may be exact because it is an average of the volumes.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
Specifications
Precision
™ Note: The specifications listed here are for typical performance.
For IQ/PQ/OQ testing purposes, add 1.0% additional tolerance to the
%CV to accommodate various test solutions, off-peak wavelengths,
reader errors, and pipette errors.
Typical 384-well dispense precision is:
•
< 2 %CV when dispensing 80 μl/well
•
< 5 %CV when dispensing 20 μl/well
Typical 96-well dispense precision is:
•
< 2 %CV when dispensing 160 μl/well using the 16-channel manifold
•
< 5 %CV when dispensing 40 μl/well using the 16-channel manifold
•
< 2 %CV when dispensing 80 μl/well using the 8-96 manifold
•
< 5 %CV when dispensing 20 μl/well using the 8-96 manifold
Typical 24-well dispense precision is:
•
< 2 %CV when dispensing 160 μl/well
•
< 5 %CV when dispensing 40 μl/well
Accuracy
Typical 384-well dispense accuracy is:
•
± 1.25% when dispensing 80 μl/well
•
± 5% when dispensing 20 μl/well
Typical 96-well dispense accuracy is:
•
± 1.25% when dispensing 160 μl/well using the 16-channel manifold
•
± 5% when dispensing 40 μl/well using the 16-channel manifold
•
± 1.25% when dispensing 80 μl/well using the 8-96 manifold
•
± 10% when dispensing 20 μl/well using the 8-96 manifold
Typical 24-well dispense accuracy is:
•
± 1.25% when dispensing 160 μl/well using the 8-24 manifold
•
± 10% when dispensing 40 μl/well using the 8-24 manifold
MicroFill Operator's Manual
65
66 | Chapter 5: Instrument Qualification
Materials
•
Corning Costar #3590 96-well plates, or equivalent (16-channel and “8-96”
manifold testing)
•
384-well plates (16-channel manifold testing)
•
24-well plates (“8-24” manifold testing)
•
Precision balance with capacity of 100 g minimum and readability of 0.01 g
resolution
•
Pipettes and graduated beakers
•
Microplate absorbance reader capable of dual wavelength reading at 630 and
405 (or 450) nm
™ If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts until you see READ IN RAPID MODE?”
•
The test solutions described on the following page. See also the important notes
below:
™ The absorbance of blue dye solutions should be measured at 630/450
(or 405) nm. The BioTek blue dye solution part number is 7773001.
™ The absorbance of yellow dye solutions should be measured at 450
(or 405)/630 nm. The BioTek yellow dye solution part number is
7120782. Note: This solution cannot be used when testing an 8-24
manifold.
™ The final absorbance for all dye solution concentrations should be in
a range between 0.700 and 1.300 OD.
™ The factory determined the pass/fail specifications for the following
tests using the test solutions described. You may choose to use your
own solutions. If any tests fail using your own solutions, however,
retry the tests using the suggested solutions.
™ Using pure DI water in place of these solutions is not recommended
and will likely result in the failure of the unit to meet specifications.
™ Liquid Test Worksheet templates can be found at the end of this
chapter for recording data reduction results. If your tests are failing,
this information will be useful for BioTek TAC to help diagnose any
problems.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
67
Test Solutions
For 16-Channel Manifold Testing:
20 µl Solution
•
If using BioTek’s blue dye solution, mix 100 ml of DI H2O with 10 ml of dye
solution to create a dilution of the concentrate. Mix 160 ml of DI H2O with
20 ml of the diluted concentrate.
•
If using BioTek’s yellow dye solution, mix 940 ml of DI H2O with 60 ml of
dye solution.
•
If using FD&C #5 yellow dye powder, mix 1 liter of DI H2O with 0.105
grams of dye powder.
80 µl Solution
•
Mix 120 ml of DI H2O with 40 ml of the 20 μl solution.
For “8-96” Manifold Testing:
20 µl Solution
•
If using BioTek’s blue dye solution, mix 100 ml of DI H2O with 10 ml of dye
solution to create a dilution of the concentrate. Then mix 140 ml of DI H2O
with 80 ml of the diluted concentrate.
•
If using BioTek’s yellow dye solution, mix 400 ml of DI H2O with 100 ml of
dye solution.
•
If using FD&C #5 yellow dye powder, mix 1 liter of DI H2O with 0.350
grams of dye powder.
80 µl Solution
•
Mix 120 ml of DI H2O with 40 ml of the 20 μl solution.
For “8-24” Manifold Testing:
40 µl Solution
•
If using BioTek’s blue dye solution, mix 140 ml of DI H2O with 12 ml of dye
solution.
•
If using FD&C #5 yellow dye powder, mix 1 liter of DI H2O with 1.64 grams
of dye powder.
•
Note: BioTek’s yellow dye solution is not concentrated enough to use for
this test.
160 µl Solution
•
Mix 120 ml of DI H2O with 40 ml of the 40 μl solution.
MicroFill Operator's Manual
68 | Chapter 5: Instrument Qualification
Dispense Precision & Accuracy Test: 16-Channel Manifold
Perform the following steps a total of four times to test two plate types (96 and 384), each
with two dye solutions.
1.
Edit the Dispense program Accuracy_Test as follows (set FLOW RATE to 2
for all tests):
VOLUME = 40 μl/well
Test 1:
PLATE TYPE = 96,
Test 2:
PLATE TYPE = 384, VOLUME = 20 μl/well
Test 3:
PLATE TYPE = 96,
Test 4:
PLATE TYPE = 384, VOLUME = 80 μl/well
VOLUME = 160 μl/well
2.
Place the priming plate onto the microplate adaptor of the MicroFill™.
3.
Prime the MicroFill™ two times with the test solution.
Tests 1 and 2:
Use the 20 μl solution
Tests 3 and 4:
Use the 80 μl solution
This will pump any residual air out of the check valves. Remove and empty the
priming plate.
4.
Place a clean, empty (96- or 384-well) microplate on the balance and then tare
the balance.
5.
Place the microplate on the MicroFill™ and run the Accuracy_Test program
modified in step 1.
6.
Place the plate on the balance and record the Total Dispense Weight.
This value will be used to calculate the % Accuracy Error in step 11.
7.
Using a calibrated hand pipette, dispense deionized water on top of the dye
solution in the wells. (Tests 1 and 2 only.)
Test 1: Pipette 120 μl/well (resulting in 160 μl/well)
Test 2: Pipette 60 μl/well (resulting in 80 μl/well)
8.
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or
lightly tap the side of the plate with your finger to agitate the contents of the
wells. This will result in a uniformly mixed solution and create a more uniform
meniscus in each of the wells.
™ If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts.
9.
Read the plate in an absorbance reader using the dual-wavelength method, to
reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
69
™ For steps 10 and 11, enter the results in copies of the Liquid Test
Worksheets at the end of this chapter.
10. Calculate and report the Mean absorbance, Standard Deviation, and the %CV
for the wells under test. %CV = (Standard Deviation ÷ Mean) * 100.
11. The % Accuracy Error calculation is:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 96-well plate, 40 μl test, Total Dispense Weight = 3.73 g:
(3.73 – 3.84) ÷ 3.84 x 100 = -2.87% = PASS (must be < ±5.0%)
The Expected Dispense Weight is the volume dispensed per well in ml
multiplied by the number of wells dispensed. For example, if 40 μl is dispensed
to 96 wells, the expected weight is 0.040 x 96 = 3.84 grams. We have calculated
some expected dispense weights for you:
Test 1 (96 wells, 40 μl/well):
3.84 grams
Test 2 (384 wells, 20 μl/well):
7.68 grams
Test 3 (96 wells, 160 μl/well):
15.36 grams
Test 4 (384 wells, 80 μl/well):
30.72 grams
12. Analyze your test results. The following is the Pass criteria for each test:
Test 1:
%CV < 6.0%*
% Accuracy Error < ± 5.0%
Test 2:
%CV < 6.0%*
% Accuracy Error < ± 5.0%
Test 3:
%CV < 3.0%*
% Accuracy Error < ± 1.25%
Test 4:
%CV < 3.0%*
% Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to
the %CV to accommodate various test solutions, off-peak wavelengths,
reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime
the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
MicroFill Operator's Manual
70 | Chapter 5: Instrument Qualification
Dispense Precision & Accuracy Test: “8-96” Manifold
Perform the following steps two times, using two different 96-well plates:
1.
Edit the Dispense program Accuracy_Test as follows (set FLOW RATE to 2
for all tests):
Test 1:
VOLUME = 20 μl/well
Test 2:
VOLUME = 80 μl/well
2.
Place the priming plate onto the microplate adaptor of the μFill.
3.
Prime the MicroFill™ two times with the test solution.
Test 1: Use the 20 μl solution
Test 2: Use the 80 μl solution
This will pump any residual air out of the check valves. Remove and empty the
priming plate.
4.
Place a clean, empty 96-well microplate on the balance and then tare the
balance.
5.
Place the microplate on the MicroFill™ and run the Accuracy_Test program
modified in step 1.
6.
Place the plate on the balance and record the Total Dispense Weight.
This value will be used to calculate the % Accuracy Error in step 11.
7.
Using a calibrated hand pipette, dispense deionized water on top of the dye
solution in the wells.
Test 1: Pipette 140 μl/well (resulting in 160 μl/well)
Test 2: Pipette 80 μl/well (resulting in 160 μl/well)
8.
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or
lightly tap the side of the plate with your finger to agitate the contents of the
wells. This will result in a uniformly mixed solution and create a more uniform
meniscus in each of the wells.
™ If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts.
9.
Read the plate in an absorbance reader using the dual-wavelength method, to
reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
71
™ For steps 10 and 11, enter the results in copies of the Liquid Test
Worksheets at the end of this section.
10. Calculate and report the Mean absorbance, Standard Deviation, and the %CV
for the wells under test. %CV = (Standard Deviation ÷ Mean) * 100.
11. The % Accuracy Error calculation is:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 96-well plate, 20 μl test, Total Dispense Weight = 1.85 g:
(1.85 – 1.92) ÷ 1.92 x 100 = -3.64% = PASS (must be < ± 10.0%)
The Expected Dispense Weight is the volume dispensed per well in ml
multiplied by the number of wells dispensed. For example, if 20 μl is dispensed
to 96 wells, the expected weight is 0.020 x 96 = 1.92 grams. We have calculated
some expected weights for you:
Test 1 (96 wells, 20 μl/well):
1.92 grams
Test 2 (96 wells, 80 μl/well):
7.68 grams
12. Analyze your test results. The following is the Pass criteria for each test:
Test 1:
%CV < 6.0%*
% Accuracy Error < ± 10.0%
Test 2:
%CV < 3.0%*
% Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to
the %CV to accommodate various test solutions, off-peak wavelengths,
reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime
the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
MicroFill Operator's Manual
72 | Chapter 5: Instrument Qualification
Dispense Precision & Accuracy Test: “8-24” Manifold
Perform the following steps two times, using two different 24-well plates:
1.
Edit the Dispense program Accuracy_Test as follows (set FLOW RATE to 2
for all tests):
Test 1:
VOLUME = 40 μl/well
Test 2:
VOLUME = 160 μl/well
2.
Place the priming plate onto the microplate adaptor of the MicroFill™.
3.
Prime the MicroFill™ two times with the test solution.
Test 1:
Use the 40 μl solution
Test 2:
Use the 160 μl solution
This will pump any residual air out of the check valves. Remove and empty the
priming plate.
4.
Place a clean, empty 24-well microplate on the balance and then tare the
balance.
5.
Place the microplate on the MicroFill™ and run the Accuracy_Test
program modified in step 1.
6.
Place the plate on the balance and record the Total Dispense Weight.
This value will be used to calculate the % Accuracy Error in step 11.
7.
Using a calibrated hand pipette, dispense deionized water on top of the dye
solution in the wells.
8.
Test 1:
Pipette 960 μl/well (resulting in 1000 μl/well)
Test 2:
Pipette 840 μl/well (resulting in 1000 μl/well)
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or
lightly tap the side of the plate with your finger to agitate the contents of the
wells. This will result in a uniformly mixed solution and create a more uniform
meniscus in each of the wells.
™ If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts.
9.
Read the plate in an absorbance reader using the dual-wavelength method, to
reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
73
™ For steps 10 and 11, enter the results in the Liquid Test Worksheets at
the end of this section.
10. Calculate and report the Mean absorbance, Standard Deviation, and the %CV
for the wells under test. %CV = (Standard Deviation ÷ Mean) * 100.
11. The % Accuracy Error calculation is:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 24-well plate, 160μl test, Total Dispense Weight = 3.64 g:
(3.64 – 3.84) ÷ 3.84 x 100 = -5.21% = PASS (must be < ± 10.0%)
The Expected Dispense Weight is the volume dispensed per well in ml
multiplied by the number of wells dispensed. For example, if 40μl is dispensed
to 24 wells, the expected weight is 0.040 x 24 = 0.96 grams. We have calculated
some expected weights for you:
Test 1 (24 wells, 40 μl/well):
0.96 grams
Test 2 (24 wells, 160 μl/well):
3.84 grams
12. Analyze your test results. The following is the Pass criteria for each test:
Test 1:
%CV < 6.0%*
% Accuracy Error < ± 10.0%
Test 2:
%CV < 3.0%*
% Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to
the %CV to accommodate various test solutions, off-peak wavelengths,
reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime
the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
MicroFill Operator's Manual
74 | Chapter 5: Instrument Qualification
Documenting Test Results
The following pages contain Dispense Precision & Accuracy Test Worksheets and
Performance Verification Logs. We recommend you make copies of the appropriate pages
and use them to record your calculations and test results.
•
Each Worksheet records calculations and pass/fail test results for an individual
test (e.g., “40 µl / 16-Channel Manifold / 96-Well Plate).
•
Each Performance Verification Log records all pass/fail test results for a
particular manifold type over time.
BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 40 µl / 96-Well Microplate
40 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 96)
% CV (SD ÷ Mean x 100)
% CV must be < 6.0%
%
‰ Pass
‰ Fail
40 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 5.0%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 160 µl / 96-Well Microplate
160 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 96)
% CV (SD ÷ Mean x 100)
% CV must be < 3.0%
%
‰ Pass
‰ Fail
160 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 1.25%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 20 µl / 384-Well Microplate
20 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 384)
% CV (SD ÷ Mean x 100)
% CV must be < 6.0%
%
‰ Pass
‰ Fail
20 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 5.0%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 80 µl / 384-Well Microplate
80 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 384)
% CV (SD ÷ Mean x 100)
% CV must be < 3.0%
%
‰ Pass
‰ Fail
80 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 1.25%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-96” Manifold / 20 µl / 96-Well Microplate
20 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 96)
% CV (SD ÷ Mean x 100)
% CV must be < 6.0%
%
‰ Pass
‰ Fail
20 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 10.0%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-96” Manifold / 80 µl / 96-Well Microplate
80 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 96)
% CV (SD ÷ Mean x 100)
% CV must be < 3.0%
%
‰ Pass
‰ Fail
80 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 1.25%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-24” Manifold / 40 µl / 24-Well Microplate
40 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 24)
% CV (SD ÷ Mean x 100)
% CV must be < 6.0%
%
‰ Pass
‰ Fail
40 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 10.0%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-24” Manifold / 160 µl / 24-Well Microplate
160 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells ÷ 24)
% CV (SD ÷ Mean x 100)
% CV must be < 3.0%
%
‰ Pass
‰ Fail
160 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
grams
Expected Weight:
(ml/well x number of wells dispensed)
grams
% Accuracy Error:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
%
% Accuracy Error must be < 1.25%
‰ Pass
‰ Fail
Visual verification that no well varies
considerably from the others
‰ Pass
‰ Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
© 2008, BioTek Instruments, Inc.
Comments:
Operator’s Initials
% Accuracy Error < 1.25%?
% CV < 3.0%?
80 μl dispense, 384-Well Microplate
% Accuracy Error < 5.0%?
% CV < 6.0%?
20 μl dispense, 384-Well Microplate
% Accuracy Error < 1.25%?
% CV < 3.0%?
160 μl dispense, 96-Well Microplate
% Accuracy Error < 5.0%?
% CV < 6.0%?
40 μl dispense, 96-Well Microplate
Date:
Performance Verification Log for the 16-Channel Manifold
MicroFill™ Serial Number: ___________
Performance Verification Log for the “8-96” Manifold
Date:
20 μl dispense, 96-Well Microplate
% CV < 6.0%?
% Accuracy Error < 10.0%?
80 μl dispense, 96-Well Microplate
% CV < 3.0%?
% Accuracy Error < 1.25%?
Operator’s Initials
Comments:
© 2008, BioTek Instruments, Inc.
MicroFill™ Serial Number: ___________
© 2008, BioTek Instruments, Inc.
Comments:
Operator’s Initials
% Accuracy Error < 1.25%?
% CV < 3.0%?
160 μl dispense, 24-Well Microplate
% Accuracy Error < 10.0%?
% CV < 6.0%?
40 μl dispense, 24-Well Microplate
Date:
Performance Verification Log for the “8-24” Manifold
MicroFill™ Serial Number: ___________
Chapter 6
Troubleshooting
This chapter lists problems that you may experience with the
MicroFill™, suggests possible solutions for these problems, and
provides instructions for receiving technical assistance.
Overview ............................................................................
General Usage Guidelines ......................................................
Before Running Any Program .............................................
Preventive Maintenance ....................................................
Programming considerations .............................................
Product Support & Service .....................................................
Returning Instruments for Service/Repair ............................
Contacting BioTek for Applications Support ..........................
Troubleshooting Charts .........................................................
Dispenser Start-Up ..........................................................
Syringe Movement ...........................................................
Fluid Delivery ..................................................................
Fluid Leakage ..................................................................
Microplate Carrier Movement .............................................
Dispense Manifold Movement.............................................
76 77 77 77 77 78 78 78 79 79 79 80 81 82 82 76 | Chapter 6: Troubleshooting
Overview
Every effort has been made to ensure that the MicroFill™ is extremely reliable and easy to
use. Nevertheless, you could experience problems with the dispenser hardware, software,
and/or accessories. This chapter offers information to help resolve these problems. This
chapter is divided into three major sections:
1.
General Usage Guidelines. When operating the dispenser, follow these
guidelines to ensure optimal performance and to prevent the problems
described in the Troubleshooting Charts. See page 77.
2.
Technical Support. If the dispenser fails to function properly, try to solve the
problem(s) by following the Troubleshooting Charts in this chapter. If the
problem persists, contact BioTek’s Technical Assistance Center (TAC). See page
78 for contact information.
3.
Troubleshooting Charts. The charts list problems, their possible causes, and
possible solutions. The charts are categorized as follows:
•
Dispenser Start-Up, page 79
•
Syringe Movement, page 79
•
Fluid Delivery, page 80
•
Fluid Leakage, page 81
•
Microplate Carrier Movement, page 82
•
Dispense Manifold Movement, page 82
™ Note: If you are operating the MicroFill™ with BioTek’s BioStack™
Microplate Stacker and encounter problems during plate transfers
between the BioStack and the dispenser, or during communication
between the two instruments, refer to the Troubleshooting and Error
Codes section of your BioStack Operator’s Manual.
BioTek Instruments, Inc.
General Usage Guidelines | 77
General Usage Guidelines
Before Running Any Program
•
Fill the supply bottles with sufficient fluid. Make sure the supply tube is in the
liquid.
•
The supply tube should extend to the bottom of the bottle and be cut
at the end (see drawing to the right) to ensure free flow of liquid at
the bottom of the supply bottle.
•
Check the external tubing connections for kinks and clogs.
•
Make sure the bottles are clean and do not contain any particles or mold.
•
If there are large air pockets in the tubing, run a Prime program using 20 ml
before running another program.
•
After running the Prime program, leave the priming plate in place and run a
Dispense program that dispenses 20 μl per well to 24 columns. This will pump
out any additional air that is still in the check valves.
•
To avoid creating air bubbles every time the supply bottle is filled, make a
mark halfway down the bottle and refill when the fluid level has dropped to
that point. Unscrew the cap and let it hang over the side just enough to avoid
emptying the inside tube and enough to refill the bottle.
•
When placing a microplate into the plate adaptor, make sure that well A1 is in
the right rear corner as you face the front of the dispenser. Make sure that the
plate is firmly seated in the adaptor and that the adaptor is firmly seated in the
carrier.
Preventive Maintenance
Perform Daily/Overnight/Monthly maintenance as described in Chapter 4.
Preventive Maintenance includes:
•
Cleaning the dispense manifold tubes and chambers
•
Cleaning the plate adaptor and carrier
•
Cleaning the supply bottles
•
Rinsing and checking the tubing and the check valves
Programming considerations
•
Ensure that the correct number of strips is specified in Dispense programs.
See “Define Dispense Program Options” in Chapter 3.
MicroFill Operator's Manual
78 | Chapter 6: Troubleshooting
Product Support & Service
A superior support staff backs all of BioTek’s products. If your instrument or software ever
fails to function perfectly, if you have questions about how to use or maintain them, or if
you need to send an instrument to BioTek for service or repair, please contact our
Technical Assistance Center (TAC).
Our Technical Assistance Center is open from 8:30 AM to 5:30 PM (EST), Monday through
Friday, excluding standard U.S. holidays. You can send a fax or an e-mail any time.
Phone:
(800) 242-4685 or (802) 655-4740
E-Mail:
tac@biotek.com
Fax:
(802) 654-0638
You can also request technical assistance from our website: www.biotek.com
Please be prepared to provide the following information:
•
Your name and company information, along with a daytime phone or fax
number, and/or an e-mail address
•
The product name, model, and serial number
•
The software configuration information. To see this information, start at the
Main Menu and select UTIL > TESTS > CHKSUM.
•
For troubleshooting assistance or instruments needing repair, the specific steps
that produce your problem and any error codes displayed on the screen.
Returning Instruments for Service/Repair
If you need to return an instrument to BioTek for service or repair, please contact the
TAC for a Return Materials Authorization (RMA) number and the shipping address.
Repackage the instrument properly (see Chapter 2, Installation), write the RMA
number on the shipping box, and ship the address provided.
Contacting BioTek for Applications Support
BioTek’s fully equipped Application Laboratory provides our on-staff scientists with
the means to assist you with the integration of our instrumentation and software with
your unique scientific applications.
Phone: (888) 451-5171
E-Mail: applications@biotek.com
BioTek Instruments, Inc.
Troubleshooting Charts | 79
Troubleshooting Charts
Dispenser Start-Up
Problem
Display (LCD) not
on.
Syringe, carrier,
or manifold
position error.
Possible Cause
Possible Solution
Power cord not plugged in.
Check power connection.
Power inlet pin pushed
together.
Gently split power inlet pin apart.
Syringe, manifold, or
carrier is being obstructed.
Remove obstruction.
Motor, sensor, or electrical
problem.
Turn Dispenser off, wait at least 15
seconds, then turn back on. If
Dispenser does not pass its self-test,
contact BioTek TAC.
Misaligned carrier or
manifold.
Contact BioTek TAC.
Syringe piston not seated
all the way to the bottom
before fastening set screw
(autoclavable only).
Reinstall the syringe head (see
Figure 2 in Chapter 2).
Carrier or carrier rail dirty.
Clean carrier and/or rail.
Syringe Movement
Problem
Possible Cause
Syringe movement is
blocked.
Syringe position
error.
Possible Solution
Check underneath the dispenser to
see if something is interfering with
the movement of the syringe.
Ensure that the syringe power cable
is connected to the PCB.
Contact BioTek TAC.
Syringe piston not seated
all the way to the bottom
before being “setscrewed”
into place (autoclavable
model only).
MicroFill Operator's Manual
Reinstall the syringe piston and
syringe head (see Figure 2 in
Chapter 2).
80 | Chapter 6: Troubleshooting
Fluid Delivery
Problem
Unable to
dispense fluid.
Plate overfills
(floods).
Possible Cause
Possible Solution
Inlet tube not connected at
manifold or at bottle.
Check all tubing (see “Tubes and
Bottles” in Chapter 2).
Supply tube inside the
supply bottle is kinked or
disconnected.
Straighten or connect supply tube
(see Figure 5 in Chapter 2). Make
sure the end is cut as shown on
page 77.
Clogged dispense tubes on
the Dispenser manifold.
Remove and clean the manifold (see
“Clean the Manifold” in Chapter 4).
Inlet tube is not connected
to the bottom port of the
syringe.
Connect the inlet tube to the lower
port of the syringe pump (see
Figure 3 in Chapter 2).
Outlet tube is not connected
to the top port of the
syringe.
Connect the outlet tube to the top
port of the syringe pump (see
Figure 3 in Chapter 2).
Check valve flow direction is
incorrect.
Compare the flow direction of the
check valves with Figure 3 in
Chapter 2. Switch or replace if
necessary.
Check valves are stuck
closed.
Clean check valves (see “Clean/
Replace the Check Valves” in
Chapter 4).
No fluid.
Fill bottles with appropriate fluid.
System not primed.
Run a Prime program using 20 ml.
Faulty syringe pump.
Contact BioTek TAC.
Set screw not tightened on
the syringe pump piston
(autoclavable model only).
Reinstall the syringe head (see
Figure 2 in Chapter 2).
Dispense height too high.
Change the Dispense Height in the
program (see “Define Dispense
Program Options” in Chapter 3).
Volume too large for the
vessel.
Program a smaller volume.
Dispense rate too fast for
volume selected.
Use slower dispense rate or lower
volume (see “Define Dispense
Program Options” in Chapter 3).
Continued on the next page.
BioTek Instruments, Inc.
Troubleshooting Charts | 81
(Fluid Delivery, continued)
Problem
Uneven
dispensing of
fluid; wells not
filled.
Dripping dispense
tubes.
Possible Cause
Possible Solution
Clogged dispense tubes on
the Dispenser manifold.
Remove and clean the manifold (see
“Clean the Manifold” in Chapter 4).
Manifold or tubing not
adequately primed (air in
fluid lines).
Run a Prime program using 20 ml.
Follow this with a dispense program
that dispenses 20 μl per well for 24
strips.
Dispense flow rate too low.
Select a higher flow rate (see
“Define Dispense Program Options”
in Chapter 3).
Setscrew not tightened on
the syringe pump piston
(autoclavable only).
Reinstall the syringe head (see
Figure 2 in Chapter 2).
Dispense tubing routed
incorrectly.
The supply bottle tube must enter
the syringe at the bottom.
Fluid Leakage
Problem
Possible Cause
Possible Solution
Replace plugs (see “Clean (“Stylize”)
the Dispense Tubes” in Chapter 4).
Defective seals.
Fluid leaking
from manifold.
Replace o-ring on Dispenser manifold
inlet fitting.
Contact BioTek TAC.
Check valves are leaking.
Clean or replace check valves (see
“Clean/Replace the Check Valves” in
Chapter 4).
Fittings to manifold are
leaking.
Reconnect/reseat the fittings (see
Figure 5 in Chapter 2).
Defective syringe cup.
Fluid leaking
from underneath
the instrument.
Leaking o-ring seal(s)
(autoclavable model only).
Contact BioTek TAC.
Leaking syringe seal.
Defective syringe piston.
Fluid leaking
from external
tubing connector.
Defective connector.
Replace fitting (see Figure 5 in
Chapter 2).
Worn tubing.
Replace tubing (see Figures 3 and
5 in Chapter 2).
MicroFill Operator's Manual
82 | Chapter 6: Troubleshooting
Microplate Carrier Movement
Problem
Possible Cause
Microplate not properly
seated or strips not level.
Dispense tubes
not entering well
correctly.
Carrier position
error.
Possible Solution
Reseat microplate in adaptor, or the
adaptor in the carrier, or the strips in
holder.
Make sure the carrier is clean (see
“Clean the Plate Carrier” in Ch. 4).
Manifold not compatible
with plate.
“8-24” manifold is not compatible
with 96-well plates. Use the “8-96”
manifold.
Horizontal Dispense
Position does not align the
tubes in the wells.
Change the Horizontal Disp Pos value
in the program (see “Define
Dispense Program Options” in
Chapter 3).
Dispense tube(s) bent.
Push the supplied stylus into the
tube and then gently attempt to
straighten the tube using your
fingers. If it remains bent, contact
BioTek TAC.
Manifold tilted.
Check tubing for twists; add manifold
screws (p/n 12174).
Carrier movement is
blocked.
Check for/remove any obstruction.
Plate Clear Height set
incorrectly.
Check the Plate Clear Height in the
program, reset as required.
Dirty carrier or carrier rail.
Clean carrier and/or carrier rail.
Dispense Manifold Movement
Problem
Possible Cause
Possible Solution
Check orientation of microplate; A1
should be in the right rear corner of
the plate carrier as you face the front
of the instrument.
Manifold position
error.
Manifold movement is
blocked.
Check for/remove any obstructions.
Ensure that the manifold is resting
on the manifold support bracket (see
Figure 1 in Chapter 2).
Contact BioTek TAC.
BioTek Instruments, Inc.
Appendix A
Error Codes
This appendix lists Error Codes that may appear on the MicroFill™.
If an error is displayed, locate it in this Appendix and then review
the charts in Chapter 6, Troubleshooting.
Overview ............................................................................
General Errors .....................................................................
BioStack Errors ....................................................................
Fatal Errors .........................................................................
84 84 87 88 84 | Appendix A: Error Codes
Overview
An error code is displayed on the MicroFill’s display as a four-digit identifier. The first
character will be 0, 1, or A. Both 0 and 1 indicate a non-critical error, in which case the
instrument will respond to keypad input. An A as the first character indicates a more
serious error. In this case, turn off the dispenser. Upon restarting, you should be able to
enter commands using the keypad.
™ Note: Error codes prefixed with a “B-“ that are displayed on the
MicroFill™during operation with the BioStack™ Microplate Stacking
System indicate errors generated by the BioStack, or problems with
communication between the two instruments. Refer to the
“Troubleshooting and Error Codes” section of your BioStack
Operator’s Manual for a list of these types of error codes and their
descriptions.
General Errors
General Errors indicate non-fatal conditions that require attention. If an error is displayed,
locate it in the table and then refer to the charts in Chapter 6, Troubleshooting. Contact
BioTek’s Technical Assistance Center if further instructions are necessary.
™ Note: For the codes that represent motor errors, the fourth digit
of the code represents the affected motor: 0 = manifold motor,
1 = carrier motor, 2 = syringe motor. For example, error code 0201
means that the plate carrier motor could not find its optical sensor.
BioTek Instruments, Inc.
General Errors | 85
Error Define
Error
Code
Brief Description
ABORT_ERR
0x0100
Current Run function aborted
NO_SENSOR_ERR
0x0200
Manifold lift motor could not find its
home optical-sensor
NO_SENSOR_ERR
0x0201
Carrier motor could not find its home
optical-sensor
NO_SENSOR_ERR
0x0202
Syringe motor could not find its home
optical-sensor
AUTOCAL_JIG_ERR
0x0300
Jig sensor state incorrect while
performing Manifold motor Autocal
AUTOCAL_JIG_ERR
0x0301
Jig sensor state incorrect while
performing Carrier motor Autocal
MOTOR_VERIFY_ERR
0x0400
Manifold motor failed positional verify
with home sensor
MOTOR_VERIFY_ERR
0x0401
Carrier motor failed positional verify with
home sensor
MOTOR_VERIFY_ERR
0x0402
Syringe motor failed positional verify with
home sensor
UNDEF_PROG_TYPE_ERR
0x0500
Undefined program type specified for
Instrument
PROGRAM_NUMBER_ERR
0x0600
Program with given number and type
does not exist
PROGRAM_SET_ERR
0x0700
No program space available,100
programs currently stored
PROGRAM_NAME_ERR
0x0800
Program with given name and type does
not exist
PROGRAM_MISSING_ERR
0x0900
Prime program required for maintenance
program missing
PROGRAM_CHECKSUM_ERR
0x0A00
Program Checksum failure
MANIFOLD_AUTOCAL_ERR
0x0B00
Invalid manifold defined in Instrument
configuration data
PROGRAM_CREATED_ONBOARD
0x0C00
Error used for serial control for programs
created on board
PROGRAM_LOCKED
0x0D00
Program locked so operation denied
PROGRAM_NONERASABLE
0x0E00
Program non-erasable so delete denied
DISPENSE_VOLUME_ERR
0x0F00
Dispense volume not within specified
limits
MicroFill Operator's Manual
86 | Appendix A: Error Codes
Error Define
Error
Code
Brief Description
INSTR_CNFG_DATA_ERR
0x1000
Instrument configuration data corrupted
INVALID_SUPPLY_ERR
0x1100
Invalid Supply index specified in program
MOTOR_NOT_HOMED_ERR
0x1300
Manifold motor not at home position
MOTOR_NOT_HOMED_ERR
0x1301
Carrier motor not at home position
MOTOR_NOT_HOMED_ERR
0x1302
Syringe motor not at home position
AUTOCAL_ABORT_ERR
0x1400
Mechanical auto-calibration was aborted
MALLOC_ERR
0x1900
Memory allocation failure
SYR_VOLUME_ERR
0x1A00
Syringe volume requested out of range
DISPENSE_POS_ERR
0x1B00
Carrier offset position is not within
defined limits
SYR_FMEA_ERR
0x1C01
Sensor not found at start of syringe
aspirate stroke
SYR_FMEA_ERR
0x1C02
Sensor not open at start of dispense
stroke
SYR_FMEA_ERR
0x1C03
Syringe aspirate stroke failure
SYR_FMEA_ERR
0x1C04
Sensor verify after last syringe stroke in
program failed
SYR_FMEA_ERR
0x1C05
Sensor clear from home position too
many steps
SYR_ULPERSTEP_ERR
0x1D00
Syringe μl/step factor is not within
specified limits
AUTOCAL_CHECKSUM_ERR
0x1E00
Autocal data corrupted or not currently
set
DISP_CAL_DATA_ERR
0x1F00
Dispense cal data checksum error
DISP_CAL_DATA_ERR
0x1F01
Dispense cal data not set
DISP_CAL_DATA_ERR
0x1F02
Dispense cal data element out of range
INVALID_LINK_ERR
0x2400
Link program invalid
BioTek Instruments, Inc.
BioStack Errors | 87
BioStack Errors
Error codes that may be displayed on the MicroFill™ when communicating with the
BioStack™:
Error Define
Error
Code
Description
INVALID_PASSWORD
0x8300
An invalid password was sent during a
protected operation.
NO_MATCHING_ITEM
0x8301
The BioStack was requested to send
some configuration data to the
controlling software, and the BioStack did
not know about the requested data.
BAD_CHECKSUM
0x8302
A checksum was generated following a
code download, which did not match
what was expected.
NOT_IN_DOWNLOAD_MODE
0x8303
The BioStack is in an invalid mode for
receiving a message.
NAK_RECEIVED
0x8304
The BioStack didn’t properly acknowledge
a message sent to it.
INVALID_RESPONSE
0x8305
Invalid message response received from
BioStack.
TIMEOUT
0x8306
The serial communications timed out.
MicroFill Operator's Manual
88 | Appendix A: Error Codes
Fatal Errors
Fatal errors indicate conditions that require immediate attention. If a fatal error is
displayed, contact BioTek’s Technical Assistance Center for further instructions.
™ Note: For the codes that represent device errors, the fourth digit
of the code represents the affected device: 0 = manifold motor,
1 = carrier motor, 2 = syringe motor, 4 = software timer,
5 = display, 6 = quick flash memory.
Error
Error
Code
Brief Description
TCB_NOT_AVAIL_ERR
0xA100
Task control block not available
Undefined
0xA200
Error is currently undefined
NOT_AVAIL_ERR
0xA300
Manifold motor device not available
NOT_AVAIL_ERR
0xA301
Carrier motor device not available
NOT_AVAIL_ERR
0xA302
Syringe motor device not available
Undefined
0xA303
Error is currently undefined
NOT_AVAIL_ERR
0xA304
Timer device not available
NOT_AVAIL_ERR
0xA305
Display device not available
NOT_AVAIL_ERR
0xA306
Configuration flash device not available
CHECKSUM_ERR
0xA400
Failed basecode checksum test on power up
POWER_ERR
0xA500
Logic supply voltage below safe level
QFLASH_TIMEOUT_ERR
0xA600
Configuration flash timed out
QFLASH_ERR
0xA700
Configuration flash read didn’t match write
Undefined
0xA800
Error is currently undefined
HEAP_CORRUPTED_ERR
0xA900
Memory heap corrupted
BioTek Instruments, Inc.
Appendix B
Chemical Compatibility
This Appendix lists the materials used in the autoclavable and
standard versions of the MicroFill™, and provides information to
assist with determining the compatibility of many dispensed
chemicals that will come in contact with the system.
Autoclavable MicroFill............................................................
Table 1. Material/Where Used List ......................................
Table 2. Chemical Compatibility Ratings ..............................
Non-Autoclavable MicroFill .....................................................
Table 3. Material/Where Used List ......................................
Table 4. Chemical Compatibility Ratings ..............................
90 90 91 92 92 93 90 | Appendix B: Chemical Compatibility
Autoclavable MicroFill
The following materials are used in the autoclavable version of the MicroFill™.
Materials listed as 1-12 in Table 1 are cross-referenced as headings 1-12 in Table 2.
Table 1. Material/Where Used List
#
Material
Where Used
1
Borosilicate Glass
Syringe cylinder, supply bottles
2
Polypropylene
Bottle cap and fittings, pre-priming trough
fitting, check valves (organic solvent tubing
set)
3
Silicone
Tubing, manifold o-rings, check valve
o-rings (autoclavable tubing set)
4
Ultem (polyetherimide)
Check valves (autoclavable tubing set)
5
316 Stainless Steel
Dispense tubes, syringe piston, cylinder
head and fittings, check valve spring
6
PTFE (polytetrafluoroethylene)
Syringe seal
7
ETFE (ethylene
tetrafluoroethylene)
Manifold fitting
8
PPS (polyphenilene sulfide)
Manifold, manifold plugs
9
EP (ethylene propylene)
Check valve o-rings (organic solvent tubing
set)
10
CPVC (chlorinated polyvinyl
chloride)
Priming trough, pre-priming trough
11
Aluminum
Carrier, microplate adaptor
12
Polystyrene
Assay plates
BioTek Instruments, Inc.
Autoclavable MicroFill | 91
Table 2. Chemical Compatibility Ratings
Material
(1-12 represent materials listed in Table 1)
Chemical
1
2
3
4
5
6
7
8
9
10
11
12
Isopropyl alcohol 70%
A
A
A
A
A
A
A
A
A
B
A
A
Ethyl alcohol 70%
A
A
B
A
A
A
A
A
A
B
A
A
Methyl alcohol 70%
A
A
A
A
A
A
A
A
A
A
A
ND
Formaldehyde 40%
A
A
B
A
A
A
A
A
A
A
B
ND
Hydrocholoric acid 20%
A
B
D
A
D
A
A
D
A
A
D
C
Hydrogen peroxide 10%
A
A
A
A
B
A
A
A
A
A
A
A
Sodium hydroxide 20%
A
A
A
A
B
A
A
A
A
A
D
A
Sodium hypochlorite <20%
A
A
B
B
C
A
A
C
B
A
D
A
Sodium hypochlorite 0.5%
A
A
B
A
B
A
A
A
B
A
D
A
Sulfuric acid <10%
A
A
C
A
B
A
A
A
B
A
B
A
Ethylene oxide
A
D
D
ND
B
A
A
D
C
C
D
C
Detergents 1%
A
A
A
A
A
A
A
A
A
A
B
A
10% Virkon
A
A
A
ND
A
ND
ND
ND
A
A
D
A
Acetonitrile
A
A
C
D
A
A
A
A
C
D
B
D
DMSO dimethylsulfoxide
A
A
C
D
A
A
A
A
B
D
A
D
Key: A = No effect; B = Slight effect; C = Moderate effect; D = Severe effect; ND = No data
™ Note: We advise against continuous contact with harsh chemicals.
It is best if the dispenser is rinsed with deionized water after contact
with any strong acid, base, or solvent.
™ Please contact BioTek with questions about compatibility of any
chemicals not described here.
MicroFill Operator's Manual
92 | Appendix B: Chemical Compatibility
Non-Autoclavable MicroFill
The following materials are used in the standard version of the MicroFill™.
Materials listed as 1-10 in Table 3 are cross-referenced as headings 1-10 in Table 4.
Table 3. Material/Where Used List
#
Material
Where Used
1
Polyethylene
Syringe seal, buffer bottle
2
Polypropylene
Fittings in bottles, inline fittings
3
Kynar (polyvinilidinefluoride,
PVDF)
Check valves
4
316 SS
Dispense tubes, syringe piston, check valve
spring
5
Noryl (polyphenylene ether
+ polystyrene)
Syringe cylinder
6
CPVC (chlorinated polyvinyl
chloride)
Manifold, microplate adaptor legs, manifold
plugs
7
Aluminum
Carrier, microplate reader
8
Polystyrene
Assay plates
9
Silicone
Tubing, manifold seal o-ring, manifold plug
o-rings
EP (ethylene propylene)
Check valve o-rings
10
BioTek Instruments, Inc.
Non-Autoclavable MicroFill | 93
Table 4. Chemical Compatibility Ratings
Material
(1-10 represent materials listed in Table 3)
Chemical
1
2
3
4
5
6
7
8
9
10
Isopropyl alcohol 70%
B
A
A
A
A
B
A
A
A
A
Ethyl alcohol 70%
B
A
A
A
A
B
A
A
B
A
Methyl alcohol 70%
A
A
A
A
A
A
A
ND
A
A
Formaldehyde 40%
D
A
A
A
A
A
B
ND
ND
A
Hydrochloric acid 20%
A
B
A
D
A
A
D
C
D
A
Hydrogen peroxide 10%
A
A
A
B
A
A
A
A
A
A
Sodium hydroxide 20%
D
A
A
B
A
A
D
A
A
B
Sodium hypochlorite <20%
A
A
A
C
A
A
D
A
B
B
Sodium hypochlorite 0.5%
A
A
A
B
A
A
D
A
B
B
Sulfuric acid <10%
A
A
A
B
A
A
B
A
C
A
Ethylene oxide
A
D
A
B
A
C
D
C
D
C
Detergents 1%
B
A
A
A
A
A
B
A
A
A
10% Virkon
A
A
ND
A
ND
A
D
A
A
A
Acetonitrile
A
A
D
A
D
D
B
D
C
C
DMSO dimethyl sulfoxide
A
A
D
A
A
D
A
D
C
B
Key: A = No effect; B = Slight effect; C = Moderate effect; D = Severe effect; ND = No data
™ Note: We advise against continuous contact with harsh chemicals.
It is best if the dispenser is rinsed with deionized water after contact
with any strong acid, base, or solvent.
™ Please contact BioTek with questions about compatibility of any
chemicals not described here.
MicroFill Operator's Manual
94 | Appendix B: Chemical Compatibility
BioTek Instruments, Inc.
Appendix C
Dispense Flow Rates
This Appendix lists valid dispense flow rates throughout the
selectable volume range.
Standard (Non-Autoclavable) Versions of the MicroFill™ .............
Autoclavable Versions of the MicroFill ......................................
16-Channel Manifold ........................................................
“8-96” Manifold ...............................................................
“8-24” Manifold ...............................................................
96 97 97 97 97 96 | Appendix C: Dispense Flow Rates
Standard (Non-Autoclavable) Versions of the
MicroFill™
Volume Range
Plate Type
Valid Rate(s)
5 – 1500 μl
384
1
10 – 3000 μl
96
1
20 – 1500 μl
384
1-2
40 – 3000 μl
96
1-2
30 – 1500 μl
384
1-3
60 – 3000 μl
96
1-3
40 – 1500 μl
384
1-4
80 – 3000 μl
96
1-4
45 – 1500 μl
384
1-5
90 – 3000 μl
96
1-5
BioTek Instruments, Inc.
Autoclavable Versions of the MicroFill | 97
Autoclavable Versions of the MicroFill
16-Channel Manifold
Volume Range
Plate Type
Valid Rate(s)
5 – 1500 μl
384
1
10 – 3000 μl
96
1
10 – 1500 μl
384
1-2
20 – 3000 μl
96
1-2
25 – 1500 μl
384
1-3
50 – 3000 μl
96
1-3
30 – 1500 μl
384
1-4
60 – 3000 μl
96
1-4
40 – 1500 μl
384
1-5
80 – 3000 μl
96
1-5
Volume Range
Plate Type
Valid Rate(s)
10 – 3000 μl
96
1-2
20 – 3000 μl
96
1-3
40 – 3000 μl
96
1-4
60 – 3000 μl
96
1-5
Volume Range
Plate Type
Valid Rate(s)
20 – 3000 μl
24
1-2
40 – 3000 μl
24
1-3
80 – 3000 μl
24
1-4
120 – 3000 μl
24
1-5
“8-96” Manifold
“8-24” Manifold
MicroFill Operator's Manual
98 | Appendix C: Dispense Flow Rates
BioTek Instruments, Inc.
Appendix D
Packing Instructions
MICROFILL PACKING INSTRUCTIONS
Failure to comply with the following packing
instructions will void the units warranty.
Follow decontamination procedure in
the manual before returning unit.
AEROSOL COVER ASBY
AEROSOL COVER
MOUNTING SCREWS
STEP 1 PACKING INSTRUCTIONS
STEP 1
STEP 1A: TURN UNIT OFF & DISCONNECT POWER SUPPLY.
DISCONNECT ALL TUBING FROM THE UNIT.
STEP 1B: REMOVE AEROSOL COVER MOUNTING SCREWS AND
REMOVE THE AEROSOL COVER ASBY. PLACE IN
PLASTIC BAG. REINSTALL AEROSOL COVER MOUNTING
SCREWS INTO THE SIDE OF THE UNIT.
AFTER REMOVING
MANIFOLD ASBY,
SCREW THUMBSCREWS
BACK INTO MANIFOLD
MOUNTING BRACKET.
CAM
STEP 3
STEP 2
CARRIER
STEP 2 PACKING INSTRUCTIONS
STEP 2A: REMOVE THE MANIFOLD. PLACE IN A 8 X 11.5 BUBBLE BAG.
STEP 2B: REMOVE THE CARRIER. PLACE IN A 8 X 11.5 BUBBLE BAG.
STEP 3 PACKING INSTRUCTIONS
STEP 3A: ROTATE CAM DOWN TO
POSITION SHOWN.
Customer Packing Instructions
Sheet 1 of 3
7141002 REV C
MANIFOLD
BRACKET
(GROOVE)
MANIFOLD
SHIPPING
BLOCK
STEP 4
STEP 4 PACKING INSTRUCTIONS
STEP 4A: ALIGN GROOVE IN SHIPPING BLOCK
AND PUSH ONTO MANIFOLD BRACKET.
STEP 4B: PLACE UNIT INTO POLY BAG.
SHIPPING BLOCK
AEROSOL COVER
BOTTLE
LOWER TRAY
BOTTLES
SHIPPING BOX
STEP 5
STEP 5 PACKING INSTRUCTIONS
STEP 5A: PLACE LOWER TRAY INTO SHIPPING BOX.
STEP 5A: PLACE BOTTLES IN LOCATIONS SHOWN.
STEP 5B: PLACE AEROSOL COVER ASSEMBLY IN LOCATION SHOWN.
STEP 5C: INSERT UNIT AND PLACE SHIPPING BLOCK INTO
SQUARE POCKETS.
Customer Packing Instructions
Sheet 2 of 3
7141002 REV C
UPPER TRAY
COVER
SERIAL CABLE
POWER SUPPLY
TUBING SET
PLACE IN A
POLY BAG
MICRO PLATE ADAPTER
ASSEMBLY AND PRIMER
PLATE INSIDE EACH OTHER,
PLACE IN POLY BAG.
MANUAL
FOR AF1000A, REMOVE
SYRINGE PUMP HEAD
INCLUDING PISTON,
PLACE IN POLY BAG.
UPPER TRAY
POWER CORD
MANIFOLD ASSEMBLY
CARRIER
STEP 5
STEP 6
STEP 6 PACKING INSTRUCTIONS
STEP 6A: INSERT UPPER TRAY INTO SHIPPING BOX.
STEP 6B: INSERT COMPONENTS INTO THE RECOMMENDED
LOCATIONS. DEPENDING ON ACCESSORIES RECEIVED
ACTUAL LOCATIONS MAY BE SLIGHTLY DIFFERENT.
NOTE: IF YOU HAVE RECIEVED AN ACCESSORY WHICH DOES NOT
FIT IN THE UPPER TRAY SPACES, THEN PACKAGE THE ITEM(S)
CAREFULLY IN A SEPERATE PACKAGE.
STEP 6C: CLOSE TOP OF BOX AND TAPE SHUT.
STEP 6C: WRITE "RMA" IN LARGE CLEAR LETTERS ON THE OUTSIDE
OF THE BOX AND RETURN TO :
BioTek Instruments, Inc
100 Tigan Street
Highland Park
Winooski, VT 05404 USA
Customer Packing Instructions
Sheet 3 of 3
7141002 REV C
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