Nikon A1r spectral - Université de Genève

Nikon A1r spectral
Imaging live (short time-lapses <2 hours)
and fixed samples
Colocalization, F-techniques
(FRAP, FRET,...)
Temperature, Humidity, CO2 control
Peristaltic pum, water bath
Optogenetic
3D Z-stacks, tiled images
- any fluorescent proteins
Point Scanning Confocal Microscopy
Multiposition time-lapse
Lasers
1
402 nm 100 mW
457 nm
471 nm
488 nm 50 mW
514 nm
561 nm
639 nm 40 mW
4
Mounted filters
2
Filter cubes for DAPI, CFP, eGFP,
RedFP and DIC analyzer
2
Bleaching
high
Resolution
good
Speed acq.
medium
Multicolor
4-8
Depth (z)
<30 um
2
3
2
1
Objectives
3
10x CFI Plan Apochromat 0.45 DT:4 mm
20x S PlanFluor ELWD 0.45 WD-8.2 -6.9
20x PlanApo VC DIC N2 NA0.8 WD=1000um
40x PlanFluor 1.3 DIC
60x Plan Apo Lambda 1.4 WD=130um
4
PhotoMultiplier Tubes (PMT detectors)
- 4 PMTs including 2 GaAsP PMTs (highly sensitive)
- 1 multispectral (spectral range from 32 channels
with a sampling 2.5, 6.0 or 10.0 nm)
Lateral Resolution:
R(x,y)= 0.4λ/NA
Axial Resolution:
2
R(x,z)= 1.4λn/NA
Bi
imaging
core facility
Faculté de Médecine
Université de Genève
Microscope
5
Nikon A1r+ microscope (Ti Eclipse inverted stand)
Resonant and Galvano scanner for fast imaging
(up to 30 fps) and conventional imaging (15 fps), respectively
Motorized XY stage, Piezo
Perfect Focus System 3 (PFS 3)
DIC prisme
6
Software
- Windows 7 64bits
- NIS Elements AR
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