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Release 1.0
Axio Zoom.V16
The Fluorescence Zoom Microscope for Large Fields
It’s About Brilliance.
Because Only the Best Is Good Enough
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
In 1994, the molecular biologist Douglas Prasher discovered that GFP was suitable
as a marker for proteins. At the time, hardly anybody suspected what this would
mean for the development of the stereo microscope: ‘stereo magnifiers’ experienced a renaissance. Scientists were now able to view complete model organisms
in fluorescence contrast. Alongside the enthusiasm for these devices, the performance of stereo and zoom microscopes has also grown noticeably, with systems
from Carl Zeiss keeping the bar high.
As we say: they are simpler, more intelligent, more integrated. They also give your
work the brilliance it deserves.
› The System
› Technology and Details
› Service
Animation
2
Axio Zoom.V16: Simpler. More Intelligent. More Integrated.
Axio Zoom.V16
Bright Fluorescence
Optimized Zoom
Intelligent Transmitted Light
The Fluorescence Zoom
in Large Fields
for a Variety of Applications
Over the Whole Zoom Range
Microscope for Large Fields
For the first time, Axio Zoom.V16 combines a
The eZoom of Axio Zoom.V16 works with a moto-
In addition to brightfield, darkfield and oblique
16x zoom with a high numerical aperture of
rized iris diaphragm coupled to the zoom. Simply
illumination, you can get an increased contrast
NA 0.25, moving to the forefront of all known
select the best mode for your purpose by pressing
brightfield at the touch of a button. With the Best
stereo and zoom microscopes. It achieves a very
a button on the SYCOP 3.
Mode button, Axio Zoom.V16 determines the
› In Brief
high aperture in the medium zoom range already:
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
actual optical state and optimizes transmitted light
you get superior fluorescence brightness in large
Brightness mode: Observe fluorescence images
automatically. Use the Adjust control to fine-tune
object fields. With Plan-NEOFLUAR Z 2.3x you can
over the complete zoom range with highest pos-
Best Mode more precisely to your application.
achieve a numerical aperture of NA 0.5 in an ob-
sible brightness.
Then simply save your setting and reload it for your
ject field of 1.5 millimeters. Use ApoTome.2 for
Eyepiece mode: This is ideal if you work mainly
next experiment - once again, at the touch of a
fluorescence imaging to obtain optical sections.
with ocular observation using conventional illumi-
button.
You get a system that masters routine screening
nation. You can zoom from large object fields with
as well as your most demanding multidimensional
maximum depth of field to high magnifications
imaging applications – Axio Zoom.V16 does it all
with maximum resolution.
brilliantly.
Camera mode: The optics of your Axio Zoom.V16
adapt to the performance of your camera. You get
an optimal relation between resolution and depth
of field across the whole zoom range.
3
Axio Zoom.V16
At the Limits: Conventional Zoom Technology
Now eZoom Images Are Twice as Sharp
The Fluorescence Zoom
The zoom body is the core of stereo and zoom
With Axio Zoom.V16, eZoom replaces the mecha-
Microscope for Large Fields
microscopes. When zooming, lenses have to be
nical curve with an electronic one. Stepping motors
positioned precisely. Until now, a metal component
position the moveable lenses precisely and take the
milled with great care would determine the exact-
tolerances of the individual lenses into account.
ness of this movement, and with it the optical
Each zoom body describes its own zoom curve and
quality of your microscope.
captures visibly more details.
› In Brief
› The Advantages
eZoom follows the base line for image sharpness
› The Applications
over the magnification range with a doubled pre-
Defocus position in µm
Your Insight Into the Technology Behind it
Magnification
cision, compared to a mechanical zoom body.
› The System
Zoom curves can be programmed individually.
› Technology and Details
depth of field curve, within these parameters images
are in focus
typical defocus curve of a single zoom channel with
mechanical zoom curve
typical defocus curve of a single zoom channel with
eletronic zoom curve
› Service
When the micro clapper of the computer-controlled glue
leveling machine brings eZoom’s lens in the zoom body
into position ...
... it is glued and cured with UV light.
From around 7,000 reference points, the zoom body
adjustment device calculates the zoom control curve.
4
Tailored Precisely to Your Applications
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
Typical applications, typical specimens
Plant biology
Developmental biology
Forensics
Screen a large number of whole model organisms,
e.g. embryos of Zebrafish and Drosophila,
Identify promising specimens,
Image with high fluorescence intensity
Find minute human
traces on large objects
Axio Zoom.V16
Fully motorized Axio Zoom.V16
Fully motorized Axio Zoom.V16
Plan-NEOFLUAR Z 1x
SYCOP 3
SYCOP 3
HXP 200 C
Plan-NEOFLUAR Z 2.3x
Plan-NEOFLUAR Z 1x
Filterset FS 38 HE
HXP 200 C
Filterset FS 38 HE, FS 43 HE
HXP 200 C
Filterset FS 27
3D - micromanipulator „aureka“ (www.auraoptik.de)
Sort and evaluate Drosophila embryos,
Acquire multidimensional images
Identify human cells,
Isolate and transport them to the PCR tube
Task
› In Brief
Observe whole living plants
› The Advantages
› The Applications
› The System
› Technology and Details
Axio Zoom.V16 provides
› Service
Example
Identify and document weakly autofluorescent
gland tissue of Nepenthes,
Isolate glands of interest
5
Your Insight into the Technology Behind It
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
ApoTome.2 – Create Optical Sections of Your
› The Applications
Fluorescent Samples
With structured illumination, you know that only
› The System
the focal plane appears in your image: ApoTome.2
recognizes the magnification and moves the appro-
› Technology and Details
priate grid into the beampath. The system then calculates your optical section from three images with
› Service
different grid positions. It’s a totally reliable way to
prevent scattered out-of-focus light, even in your
thicker specimens. You get images with high contrast in the best possible resolution – simply brilliant
optical sections.
Animation from www.zeiss.com/campus, © Mike Davidson, FSU, Tallahassee
A
B
C
D
ApoTome.2 Grid in the Beampath
Fluorescence excitation light passes through two glass plates in the ApoTome.2 slider. When a grid structure
is applied to the first glass plate, the grid pattern is “imprinted” in the excitation light. A scanning mechanism
tilts the second glass plate and the image of the grid is laterally shifted in the focal plane of the specimen.
Schematic illustration of the grid projection.
A-C: raw images with different positions of grid
D: optical section through sample
www.zeiss.com/campus
6
Axio Zoom.V16: A Flexible Choice of Components
Axio Zoom.V16
Axio Zoom.V16 for fluorescence screening
4
The Fluorescence Zoom
Microscope for Large Fields
1 Microscope
• Axio Zoom.V16 microscope body
• Binocular ergo phototube Z 0-30° mot
› In Brief
with PL 16x/16 eyepieces
• Stand base 450
› The Advantages
• Focus motor 3 with profile column
• Objective nosepiece Z, 2x cod
› The Applications
3
• Mechanical stage S 150x100 mot; CAN
• System Operator Panel SYCOP 3 with
› The System
Controller EMS 3
1
• Option: ApoTome.2 module
5
› Technology and Details
2 Objectives
› Service
• Plan-NEOFLUAR Z 1.0x/0.25 FWD 56mm
• Plan-NEOFLUAR Z 2.3x/0.57 FWD 10.6mm
2
3 Illumination
• Fluar Illuminator Z mot
• Reflector modules Z FL P&C (ACR or ACR RW)
• HXP 200 C (metal halide)
3
• Transillumination top 450 mot (brightfield,
brightfield+, darkfield, oblique illumination)
4 Cameras
5 Software
• AxioCam HRm
Recommended ZEN modules:
• AxioCam MRm
• Multi Channel, Z Stack, Time Lapse (imaging)
• Tiles & Positions (imaging with scanning stage)
7
Axio Zoom.V16: A Flexible Choice of Components
Axio Zoom.V16
Axio Zoom.V16 for fluorescence imaging
The Fluorescence Zoom
Microscope for Large Fields
1 Microscope
• Axio Zoom.V16 microscope body
• Binocular phototube Z 15°
› In Brief
with PL 10x/23 eyepieces
• Transmitted light base 300
› The Advantages
• Coarse/fine drive with profile column 490mm
• Mount S with diameter 76mm
› The Applications
1
2 Objective
› The System
• Objective Plan-NEOFLUAR Z 1.0x/0.25 FWD
56mm
› Technology and Details
3 Illumination
› Service
• Fluar Illuminator Z mot
• Reflector modules Z FL P&C
• HXP 200 C (metal halide)
2
• Brightfield, darkfield, oblique transmitted light
3
8
Axio Zoom.V16: System Overview
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
9
Axio Zoom.V16: System Overview
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
10
Axio Zoom.V16: System Overview
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
11
Technical Specifications
Axio Zoom.V16
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
› The System
›
Technology
and Details
› Service
Objective
FWD
(mm)
Eyepiece PL 10x/23
Magnification
Object field (mm)
Eyepiece PL 16x/16
Magnification
Object field (mm)
PlanApo Z 0.5x
Parfocal length 164 mm
114
3.5x ... 56x
66 ... 4.1
5.6x ... 90x
46 ... 2.9
PlanApo Z 1.0x
Parfocal length 164 mm
60
7x ... 112x
33 ... 2.0
11x ... 179x
23 ... 1.4
Apo Z 1.5x
Parfocal length 133 mm
30
10.5x ... 168x
22 ... 1.4x
16.8x ... 269x
15.2 ... 0.95
PlanNEOFLUAR Z 1.0x
Parfocal length 133 mm
56
7x ... 112x
33 ... 2.0
11x ... 179x
23 ... 1.4
12
Count on Service in the True Sense of the Word
Axio Zoom.V16
Your results really matter to us: we want you to get the best you expect from your microscope. Depend on
The Fluorescence Zoom
Carl Zeiss for everything you need: technology, software, advice and service. We stay with you long after
Microscope for Large Fields
installation of your microscope on site. ZEISS specialists will continue to maintain your systems, repair them,
supply spare parts and much more. Just call us: we are always here for you.
› In Brief
Total Protection with Your Carl Zeiss Service Contract
It’s the safe and practical way to preserve the efficiency of your microscope system. Our service contract
› The Advantages
protects you against expensive downtime.
› The Applications
Preventive Maintenance Plus Optimizes Performance
Our specialists will maintain and tune your system at regular intervals. You get valuable advice and
› The System
comprehensive answers to any and all questions. We will also keep you right up to date on developments
in your field of application.
› Technology and Details
The Standard Contract Also Includes Repairs and Support
› Service
In addition to all the services of Preventive Maintenance Plus, the standard contract covers all repair and
support services. The only costs you will ever pay are for replacement components. Another important
aspect of the standard contract is installation of software updates – your system will always be running
the latest program.
The Premium Contract Covers Spare Parts Too
Opt for the premium contract and you will have all services of the standard contract, plus free spare parts.
This means you can predict your running costs precisely – and budget for them.
We are here for you:
www.zeiss.com/microservice
13
Axio Zoom.V16
The moment technology provides
you with a result the first time.
This is the moment we work for.
The Fluorescence Zoom
Microscope for Large Fields
› In Brief
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
// CONFIDENCE
MADE BY CARL ZEISS
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The Fluorescence Zoom
Microscope for Large Fields
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› In Brief
› The Advantages
› The Applications
› The System
› Technology and Details
› Service
Carl Zeiss Microscopy GmbH
07745 Jena, Germany
Biosciences
microscopy@zeiss.com
www.zeiss.com/axiozoom
60-2-0074e CZ-IX/2011 | Design, scope of delivery and technical progress subject to change without notice. | © Carl Zeiss MicroImaging GmbH
Axio Zoom.V16
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