More insight to the single cell genome. Blood Banking AmpliGrid Single Cell PCR System Capillary Electrophoresis Centrifugation Flow Cytometry Genomics Lab Automation Lab Tools Particle Characterization • Consists of AmpliGrid slide and AmpliSpeed thermal cycler • Increases single cell sensitivity by a factor of five compared to tube PCR • Reduces reagent consumption by a factor of 10 – 20 • Eliminates template loss and amplification bias • Designed for highly multiplexed PCR and gene expression assays on individual cells • Allows for optical quality control during sample processing • Fits into cell sorters for reliable single cell deposition and quality control • Streamlines workflow and reproducibility • Complete line of PCR reagents and primer design services available “Clearly, the sensitivity of the AmpliGrid system allows the detection of single viral sequences in single cells even against a thousand-fold background of non-infected cells.” Norman Haefner, PhD Department of Gynecology (Gynecological Molecular Biology) Jena University Hospital, Germany DS-14756B AmpliGrid Platform Specifications 2,3 ±0,0 Dimensions 75,6 ±0,2 1,6 ±0,1 4,5 ±0,0 12,0 ±0,1 3,0 ±0,1 25,0 ±0,2 AG480F A B C D serial#1 1 2 3 4 5 6 7 8 9 10 11 12 AmpliGrid 4,5 ±0,0 5,75 ±0,1 18,0 ±0,0 8,5 ±0,0 3,5 ±0,1 lay-on edge AmpliGrid Single Cell PCR Slide AmpliSpeed Thermal Cycler Size: 25 mm x 75.6 mm (+/– 0.2 mm) Material: Ultra-flat glass microscope slide Number of reaction sites: 48 Diameter reaction sites (hydrophilic area): 1.6 mm (+/– 0.1 mm) Diameter reaction sites (hydrophobic area): 3.0 mm (+/– 0.1 mm) Features: Inactivated, DNA-free Volume aqueous phase: 1 μL (+/– 10 %) Volume sealing solution: 5.0 μL Pitch reaction sites: 4.5 mm (+/– 0.0 mm) Production facility class: 100 cleanroom Temperature range: Ambient +15°C to 99°C Temperature accuracy: +/- 0.2°C at 72°C Temperature homogeneity: +/- 0.2°C after 20 seconds at 72°C Effective heating rate: 3°C/sec. Effective cooling rate: 3°C/sec. Dimensions [mm] (wxdxh) ASC200D/400D : 126 x 295 x 112 / 126 x 486 x 112 Weight ASC200D/400D: 2.9 kg / 4.7 kg Operation conditions: +10°C to + 30°C Ordering Information DNA-free microliter reaction slide (5 pcs./pck.), 48 reaction sites each, incl. 1 x 4 mL Sealing Solution B11228 DNA-free microliter reaction slide (5 x 5 pcs.), 48 reaction sites each, incl. 4 x 4 mL Sealing Solution (for manual use) OAX04505 AmpliSpeed two bay slide cycler instrument with display unit, incl. 1x AmpliTray OAX04102 AmpliSpeed ASC400D AmpliSpeed four bay slide cycler instrument with display unit, incl. 1x AmpliTray OAX04103 AmpliGrid RT-PCR Cell Sorting Starter Set 1x AmpliSpeed ASC200D, 5 x 5 pcs. AG480F, 1x Flow Cytometry Calibration Slide, 5x One-step Single Cell RT-PCR Kit , 4 x 4 mL Sealing Solution A98027 ASC400D-B3K Biomek* 3000 integration set: ASC400D slide cycler mounted and leveled on carrier including all accessories and software CD B00278 AmpliGrid Single Cell RT-PCR Kit AmpliGrid Single Cell One-step RT-PCR system. Sufﬁcient for 5 AG480F slides. No AmpliGrid slides included. AmpliGrid AG480F AmpliSpeed ASC200D OAX04515 www.coulterﬂow.com Space for FSC Logo For Research Use Only. Not for use in diagnostics procedures. Beckman Coulter, the stylized logo, AmpliGrid , AmpliSpeed and Biomek are trademarks of Beckman Coulter, Inc. and are registered with the USPTO. Printed with soy ink For Beckman Coulter’s worldwide ofﬁce locations and phone numbers, please visit “Contact Us” at www.beckmancoulter.com B2011-12769-DG-5000 © 2011 Beckman Coulter, Inc. PRINTED IN U.S.A.
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