gel documentation and analysis

gel documentation and analysis
GEL DOCUMENTATION
AND ANALYSIS
Automated imaging
GEL IMAGING AND ANALYSIS
Automated imaging for all your applications
Syngene imaging systems are
recognised world-wide as high
quality, high performance
instruments for the image capture
and analysis of fluorescent gels,
stain-free gels, chemiluminescent
Western blots, bioluminescence
and protein samples. With over
30 years of experience, Syngene
systems are respected for their
reliability, accuracy, intuitive
software and innovative features.
Versatile
The G:BOX range has a number of variants, each designed to perform to the
highest level for specific applications. With two different darkroom designs,
each system uses a camera/lens assembly specifically chosen to produce the
best possible images for the chosen application. Lighting configurations
for the darkroom cover a wide range of sample types and applications
ranging from fluorescence, chemiluminescence and visible
light studies.
Powerful
At the heart of each G:BOX is the GeneSys control
software which takes automation to a new level.
With a G:BOX system the user can elect to use
full automated control by simply telling the G:BOX
the application and GeneSys does the rest. Using
the extensive database, the system is able to
calculate the best lighting, filter and camera
configuration for any dye or substrate being used.
The final result is a high quality, quantifiable
image at the touch of a button.
Unique
Syngene uses an unique range of CCD cameras - exclusive
to us. In addition to the high sensitivity and resolutions,
Syngene cameras have exceptional levels of quantum efficiency
for superb image quality.
2
CONTENTS INTRODUCTION 2 G:BOX HIGHLIGHTS 4
G:BOX SYSTEMS 6 POWERED BY GENESYS 8 G:BOX APPLICATIONS 10
GEL AND BLOT ANALYSIS WITH GENETOOLS 12 GENEDIRECTORY 13 FILTERS 14
G:BOX ACCESSORIES 15 G:BOX FEATURES AND BENEFITS 16 G:BOX SPECIFICATIONS 17
“The G:BOX range has a number of variants,
each designed to perform to the highest
level for specific applications”
3
G:BOX HIGHLIGHTS
What makes a G:BOX system special
Cameras
Each G:BOX uses a camera suitable for
specific applications. These cameras cover
a range of resolutions with the majority
having Peltier cooling to reduce electronic
background noise, allowing for increased
exposure times to be used which are
sometimes required for faint gels
and blots.
Lenses
Each camera is matched with a specific
lens for optimum image quality.
Lenses are controlled by the GeneSys
control software with most having
data feedback and auto focus.
Aperture control, focus and zoom
position are controlled from the
computer screen using GeneSys.
Filter wheel
A motor driven filter wheel is standard
across the G:BOX range. This 7 position
filter wheel enables an extensive range
of emission filters to be used for an
array of applications. Filter selection is
controlled by GeneSys. An orange UV
filter is supplied as standard.
See filter list for the full range of options.
Adjustable stage
G:BOX has two darkroom designs.
For the G:BOX F3, and the Chemi XRQ
the standard sized darkroom is used and
for the G:BOX Chemi XT4, XX6 and XX9
models the ‘extended’ version of the
darkroom is utilised. This extended darkroom
has a fully variable motor driven stage which
is controlled by GeneSys. This enables samples
to be moved closer to the camera and is especially
useful when working with smaller format gels and
blots. Being closer to the lens also helps when
looking at low output gels and blots, allowing greater
light capture by the camera. The stage is linked with
GeneSys so that the lens automatically ‘tracks’ the
sample as it is moved up and down, providing an
auto-focus function.
4
Internal lighting
White light
Standard in every G:BOX is an integral white LED EPI
lighting system for the viewing of visible light blots,
sample positioning and when imaging colorimetric
markers on Western blots.
EPI ultra violet light option
When overhead EPI UV is required for blots and gels or
other sample types, a UV module with either a 254nm,
302nm or 365nm UV tube can be fitted on either side of
the darkroom.
LED EPI lighting options
To extend the range of applications, the G:BOX can use
a unique ‘plug and play’ lighting system with red, green,
blue and IR LEDs. The compact lighting modules are
single wavelength devices with integral excitation filters
which simply clip into a gantry which is fitted to both
sides of the darkroom.
Up to 4 modules can be plugged into each side of the
darkroom, either of the same or of mixed wavelengths.
There are a number of different LED modules to choose
from. Standard modules have cut-off filters and
generally produce a wider output wavelength whereas
the ‘M’ series modules are fitted with special ‘narrow
band’ pass filters which allow for the imaging of
multiplexed blots with minimal ‘cross-talk’ between dyes.
Visible transmitted light options
For transmitted light applications such as Coomassie
and silver stain gels, a conversion screen is available.
This screen is placed over the UV transilluminator to
produce a large, evenly illuminated white light area.
White light pad options
An electronic white light pad is available as an option for
users who prefer to use this method for white light rather
than a visible light converter screen. Different sizes are
available to suit different sample sizes – 20 x 14cm and
20 x 30cm are available.
UV transilluminator option
A 302nm UV transilluminator can be used
to excite a range of gel types including Ethidium
bromide stained DNA gels and stain-free gels.
The transilluminator slides in and out of the darkroom
on rollers for easy access. Other wavelengths including
dual wavelengths are available.
Blue light transilluminator
An LED transilluminator operating at 470nm is suitable
for use with a range of dyes including all the ‘safe-dyes’
which are used as an alternative to Ethidium bromide.
The UltraBright Blue LED unit has high output
illumination for many applications.
5
G:BOX
Choose your
system
Applications index
DNA GELS
MULTIPLEX
GELS - 3 or
more colours
2D GELS
(DIGE)
6
PROTEIN
GELS
COLONY
COUNTING
CHEMILUMINESCENCE
BLOTS
COLORIMETRIC
BLOTS
STAIN FREE
IMAGING
FILMS
INFRA RED
IMAGING
BIOLUMINESCENCE
GFP PLANT
IMAGING
G:BOX F3
G:BOX Chemi XRQ
Affordable system for routine
fluorescent and visible gel
imaging
Affordable system for
fluorescent and visible gels and
chemiluminescent Western blots
High resolution 3.8m pixel camera
for good spatial resolution
High resolution, High QE 73% @
425nm 4m pixel images with
enhanced cooling for longer
exposure times - suitable for
chemiluminescence
Effective resolution up to 15.3m
pixels
Effective resolution up to 16m
pixels
Motor driven lens with feedback
Motor driven lens with feedback
Integral LED white lighting for
sample positioning, visible light
applications and colorimetric
markers
Integral LED white lighting for
sample positioning, visible light
applications and colorimetric
markers
Motor driven 7 position filter
wheel with UV filter to extend
applications
Motor driven 7 position filter
wheel with UV filter to extend
applications
Automatic or manual control
using GeneSys
Automatic or manual control
using GeneSys
Pre-defined ‘one click’ protocols
for speedy imaging
Pre-defined ‘one click’ protocols
for speedy imaging
Unlimited user saveable protocols
Unlimited user saveable protocols
Use with UV transilluminator for
DNA imaging - use converter
screen for visible light applications
such as protein gels
Use with UV transilluminator for
DNA imaging - use converter
screen for visible light applications
such as protein gels
Use with Blue LED UltraBright
transilluminator for ‘safe-dye’
applications
Use with Blue LED UltraBright
transilluminator for ‘safe-dye’
applications
Includes unlimited copies of
GeneTools analysis software
Includes unlimited copies of
GeneTools analysis software
IQ/OQ qualification protocols
available as optional
IQ/OQ qualification protocols
available as optional
Upgradeable to Chemi XRQ
Built-in light gantries for LED EPI
illumination options
G:BOX Chemi XT4
G:BOX Chemi XX6
G:BOX Chemi XX9
Optimum performance system.
Highly sensitive for advanced
chemiluminescent Western blots,
multiplex gels and routine
imaging of fluorescent and visible
gels and blots
Optimum performance system.
Highly sensitive for advanced
chemiluminescent Western blots,
multiplex gels, IR and routine
imaging of fluorescent and visible
gels and blots. Higher spatial
resolution for advanced
applications including 2D
Optimum performance system.
Super high sensitive for advanced
chemiluminescent Western blots,
multiplex gels, IR and routine
imaging of fluorescent and visible
gels and blots. Optimum spatial
resolution for advanced
applications including 2D
High resolution 4.2.m pixel camera
with high sensitivity. Advanced
cooling for very long exposure
times
Super high resolution with 6m
pixel camera having QE greater
than 73% @ 425nm giving
outstanding sensitivity for
chemiluminescence
Optimum high resolution with 9m
pixel camera having QE greater
than 73% @ 425nm giving
outstanding sensitivity for
chemiluminescence
Effective resolution up to 16.76m
pixels
Effective resolution up to 18m
pixels
Effective resolution up to 27m
pixels
Motor driven lens with feedback
Motor driven lens with feedback
Motor driven lens with feedback
Integral LED white lighting for
sample positioning, visible light
applications and colorimetric
markers
Integral LED white lighting for
sample positioning, visible light
applications and colorimetric
markers
Integral LED white lighting for
sample positioning, visible light
applications and colorimetric
markers
Motor driven 7 position filter
wheel with UV filter to extend
applications
Motor driven 7 position filter
wheel with UV filter to extend
applications
Motor driven 7 position filter
wheel with UV filter to extend
applications
Automatic or manual control
using GeneSys
Automatic or manual control
using GeneSys
Automatic or manual control
using GeneSys
Pre-defined ‘one click’ protocols
for speedy imaging
Pre-defined ‘one click’ protocols
for speedy imaging
Pre-defined ‘one click’ protocols
for speedy imaging
Unlimited user saveable protocols
Unlimited user saveable protocols
Unlimited user saveable protocols
Use with UV transilluminator for
DNA imaging - use converter
screen for visible light applications
such as protein gels
Use with UV transilluminator for
DNA imaging - use converter
screen for visible light applications
such as protein gels
Use with UV transilluminator for
DNA imaging - use converter
screen for visible light applications
such as protein gels
Use with Blue LED UltraBright
transilluminator for ‘safe-dye’
applications
Use with Blue LED UltraBright
transilluminator for ‘safe-dye’
applications
Use with Blue LED UltraBright
transilluminator for ‘safe-dye’
applications
Includes unlimited copies of
GeneTools analysis software
Includes unlimited copies of
GeneTools analysis software
Includes unlimited copies of
GeneTools analysis software
IQ/OQ qualification protocols
available as optional
IQ/OQ qualification protocols
available as optional
IQ/OQ qualification protocols
available as optional
Built-in light gantries for LED EPI
illumination options
Built-in light gantries for LED EPI
illumination options
Built-in light gantries for LED EPI
illumination options
Fully variable motor driven sample
stage to accommodate large to
small gel formats. GeneSys
controlled with auto focus
Fully variable motor driven sample
stage to accommodate large to
small gel formats. GeneSys
controlled with auto focus
Fully variable motor driven sample
stage to accommodate large to
small gel formats. GeneSys
controlled with auto focus
Powerful automatic mulitplex
capture protocol using red, green
or blue LED EPI modules
Powerful automatic mulitplex
capture protocol using red,
green or blue LED EPI modules
Powerful automatic mulitplex
capture protocol using red,
green or blue LED EPI modules
Add IR Multiplex kit, EPI lighting
modules and filters to image IR
blots using dyes from LI-COR®
and DyLight
Add IR Multiplex kit, EPI lighting
modules and filters to image IR
blots using dyes from LI-COR®
and DyLight
Add IR Multiplex kit, EPI lighting
modules and filters to image IR
blots using dyes from LI-COR®
and DyLight
Add Edge Lighting unit for 2D gel
imaging (DIGE)
Add Edge Lighting unit for 2D gel
imaging (DIGE)
Add Edge Lighting unit for 2D gel
imaging (DIGE)
7
POWERED BY GENESYS
Applications at the touch of a buttonplications
GeneSys control software
GeneSys is unique, intuitive software for the control and
image capture of any Syngene system including all G:BOXs. Unlike other capture software, GeneSys is
‘application driven’. At the heart of GeneSys is an
extensive database which contains an impressive list
of dye and protocol data. The user only needs to tell
GeneSys which application they are using along with
their gel or blot size and the software will automatically
configure the system for that application.
The resulting captured images are of exceptional quality
and can be reproduced time after time. GeneSys is a
truly unique application driven software unequalled
by others.
The G:BOX can be used in a fully automated mode which
means the user needs no experience in image capture.
For those who wish to select their own settings a manual
mode is available. To automate the G:BOX further the
user can save their own protocols on the Home page.
Protocols save the sample type, dyes, lighting and filters
used and the position of the iris/focus and sample size
so the user ‘clicks’ only one button to go from sample
positioning to image capture. This is especially useful
when running a number of repeat applications.
“GeneSys is a
truly unique
application
driven software
unequalled by
others”
When it comes to multiplexing, GeneSys provides an
impressive protocol which takes care of all lighting, filter
and exposure settings for each fluorophore. Up to 5
different fluorophores can be imaged at a time which
can then be displayed as a multichannel image, as a
colour overlay or as single images. No other system
can give you such an automated process of multiplexing
applications.
For Western blot applications GeneSys automatically
determines the optimum settings for perfect images
using any chemiluminescent reagent. Single images or
a series of timed images can be captured if required.
You can also image molecular weight or colorimetric
markers which are automatically overlaid with your
chemiluminescent image.
GeneSys has a number of automatic functions which
help the image capture process to produce perfect,
high quality images.
8
Effective resolution
The effective resolution on each G:BOX system can
be used to output larger resolution images. This is
especially useful for publication purposes.
Flatfix technology
This is Syngene’s unique algorithm which perfects image
capture. Cameras have automatic correction to eliminate
any hot pixels or imperfections. This leaves the image
background clear from any ‘speckles’ or ‘spots’ which
would degrade the image quality.
Neutral fielding
When working with white light backgrounds a neutral
fielding process can be used to give perfect ‘flat’
backgrounds to images.
Auto gamma control
Another automatic function is a gamma control which
sets the black and white levels of the captured image
to an optimum setting. This results in improved
definition between bands or spots and the
background of the image.
Select gel or
blot or manual
mode
Choose from any saved
configurations for faster imaging
9
G:BOX APPLICATIONS
Applications you can image with a G:BOXplications
Multiplex fluorescence detection
G:BOX Chemi systems offer enhanced sensitivity
ensuring that the broad dynamic range available with
fluorescence is captured providing exceptional linearity
for accurate quantification.
GeneSys image capture software can effortlessly
detect multiple proteins simultaneously.
Detect up to five different fluorophores on the same
gel or blot. Band intensity values can be normalised to
another protein or a loading control eliminating the need
to strip and re-probe.
GeneSys software automatically overlays image data
from each fluorescent channel whilst allowing the user to
view individual fluorescent channels to detect
overlapping bands.
Table I
System
Detection
limit
(LOD) (µg)
Linearity (R2)
Blue
Green
Red
channel channel channel
G:BOX Chemi XX9
0.0078
0.99
0.98
0.97
G:BOX Chemi XX6*
0.0078
0.97
0.96
0.97
G:BOX Chemi XT4*
0.0078
0.97
0.98
0.98
*Data not shown
Figure 1 - Multiplexed fluorescent Western blot
Proteins BSA, -cassein and RNase A were diluted two-fold
ranging from 1µg to 7.8ng. The gel was probed with
G-Dye100, G-Dye200 and G-Dye300 (NHDyeAgnostics, GmbH).
Signal Intensity was calculated by raw volume using
GeneTools analysis software (Syngene, UK). Images were
captured using GeneSys image capture software on a G:BOX
Chemi XX9 system.
R2 and LOD values are shown in Table I.
10
Stain-free imaging
Stain-free technology removes extra steps and long delays from staining. The stain-free protocol has comparable
sensitivity to that of more traditional techniques such as, Coomassie Blue Safe staining.
All G:BOX systems are stain-free ready and with GeneSys software it has never been easier to capture perfect images
of your stain-free gels.
Figure 2 - Stain-free gel imaging compared to ProtoBlue safe staining.
Serial dilutions (338-2.64ng) of a protein mixture (BSA, Carbonic anhydrase and Lysozyme) were run on a Criterion
4-20% TGX Stain-Free gel and imaged with UV on a G:BOX Chemi system and additionally stained with ProtoBlue Safe
stain. The linearity and sensitivity of the stain-free method is comparable to the ProtoBlue Safe stain method.
Chemiluminescence
With GeneSys, automatic imaging of
chemiluminescent blots has never been easier.
Take the guess work out of imaging and let GeneSys
determine the optimal exposure depending on whether
a speedy image or a high quality image is required.
Achieve dynamic ranges that surpass film to ensure fully
quantifiable data with all G:BOX Chemi systems.
Ensure that with GeneSys software you never saturate
your bands and easily capture visible markers that
are overlaid with your chemiluminescent image for
molecular calculation.
Table II
Dynamic range
System
Detection Linearity Orders of
(R2)
magnitude**
limit
(LOD) (µg)
Range
G:BOX Chemi XX9*
0.16µg
0.9531
1.5
5.1-0.16µg
G:BOX Chemi XX6
0.16µg
0.975
1.5
5.1-0.16µg
G:BOX Chemi XT4*
0.32µg
0.9585
1.2
5.1-0.32µg
G:BOX Chemi XRQ*
0.32µg
0.9817
1.2
5.1-0.32µg
Figure 3 - Chemiluminescence Western blot
Jurkat whole cell lysate was diluted 2-fold
(5.1-0.16µg) probed with Ferrochelatase
followed with anti-mouse HRP secondary
antibody and incubated with ChemiFast
chemiluminescence substrate. Signal
intensity was calculated from raw volume
using GeneTools analysis software
(Syngene, UK). Image was captured on a
G:BOX Chemi XX6 system.
R2 and LOD values are shown in Table II.
*Data not shown **calculated over data range shown
The G:BOX range are versatile systems capable of imaging a wide range of applications from DNA detection with
Ethidium bromide or ‘safe’ dyes to protein analysis of Coomassie blue stain or silver stained gels to fluorescent stained
gels and blots e.g. Chemiluminescence, QDots, DyLight , Alexa Fluor, Cy Dyes, and LI-COR© IR dyes, bioluminescence
and GFPs as well as stain free imaging.
11
GEL AND BLOT ANALYSIS
WITH GENETOOLS
Automated analysis at the click of a buttonplicaGeneTools analysis software provides rapid and
powerful gel analysis. Automatic detection of lanes and
bands, viewing densitometry profiles and user-friendly
functions allow for customisation and adjustments to be
made.
It has never been easier to analyse multiplexed gels or
blots with GeneTools making it possible to view and
analyse overlaid channels to automatically detect bands
in separate channels at the same time and to also view
individual channels.
Share and export data easily. Save, open and analyse
image files, export data reports directly to Microsoft
Excel and Word.
“It has never been
easier to analyse
multiplexed gels
or blots”
Accurately quantify a
multiplexed Western
blot using GeneTools
Automatically detect
lanes and bands and
easily add molecular
weight ladders with
GeneTools
•1D gel analysis •MW/BP calculation •Quantification •E-gels •Band matching with dendrograms •Spot blots
•Colony counting •GeneDirectory (option) for extended band matching, cluster analysis, VNTR analysis,
genotyping, RFLP studies, dendrogram generation and bootstrapping.
12
GENEDIRECTORY
Data storage and extended band matching software
GeneDirectory is a must for anyone running large
numbers of 1D gels and who needs to make inter-gel
comparisons. Using the data output from GeneTools,
the 1D gel analysis package, GeneDirectory acts as a
database for track and band results and as a tool to
quickly produce relationship dendrograms.
Key features are:
Versatile storage and comparison facilities
User-defined libraries based on classification data
Methods for combining multiple patterns from any gel,
experiment or library
Band matching across multiple experiments,
showing dendrograms, matching matrix tables,
coefficient tables and matching results
“Inter-gel
comparison
and dendrogram
generation at
the click of
a button”
Ability to search the entire database for matching
tracks based on band or classification similarities
Complex co-dominance and dominance analysis
Generations of cluster analysis diagrams
Secure storage of data in a fully GLP compliant
relational database
Direct export of data to Excel™ spreadsheets, Word™
or text files
View track information
and dendrograms side
by side
Matching
co-efficients
table
13
FILTERS
Emission filters for use with an
impressive choice of applications
All G:BOXs are supplied with an orange UV filter as
standard, fitted into the motor driven filter wheel.
To extend the number of applications that can be
used with the G:BOX, a range of emission filters
are available. These can be fitted to the filter
wheel as required.
14
Filter
Wavelength range nm
Description
UV06
572-625
UV filter for G:BOX Chemi systems (standard)
UV032
572-630
UV filter for G:BOX F3 (standard)
SW06
516-600
Short pass filter for G:BOX Chemi systems
SW032
515-599
Short pass filter for G:BOX F3
LW06
611-641
Long pass filter for G:BOX Chemi systems
LW032
615-644
Long pass filter for G:BOX F3
FILT440
427-457
Filter
FILT525
516-539 peak 525
Multiplexing filter for blue light applications
FILT565
556-579 peak 565
Filter
FILT605
590-607 peak 605
Filter
FILT605M
594-610 peak 605
Multiplexing filter for green light applications
FILT620
600-640 peak 620
Filter
FILT655
633-660 peak 655
Filter
FILT705
697-717 peak 705
Filter
FILT705M
700-720 peak 705
Multiplexing for red light applications
FILT800
780-820 peak 800
Filter
FRLP
670-780
Red light applications
IR780
782-900
IR applications and more Far-Red applications
LY800
809-876
IR multiplexing filter suitable for IR dye 800 and DyLight 800
NEUTRAL
DENSITY
N/A
For neutral fielding epi LED modules
ACCESSORIES AND OPTIONS
Extending the range of imaging applications
UltraBright LED blue light
transilluminator
The UltraBright LED blue light transilluminator can be
used with the G:BOX for those applications requiring
blue light excitation e.g. ‘safe’ dyes.
The UltraBright can be used within
the darkroom on a sliding tray.
The unit has a dual array of
high intensity blue light
470nm LEDs, powerful
enough to excite all
gels. Once connected
to the G:BOX, control
of this lighting is taken
care of by GeneSys.
Visible light converter screen
For extending the transmitted light applications such
as coomassie gels and silver
stain gels, a converter
screen is available. This
screen is placed over
the UV transilluminator
to produce a large,
evenly illuminated
white light area.
White light pad
Transilluminator
A 302nm UV transilluminator can be used to excite a
range of gel types including Ethidium bromide stained
DNA gels. The transilluminator slides
in and out of the darkroom on
rollers for easy access.
Various wavelengths
and sizes are available.
(See ordering
information on website
for further information).
An electronic white light pad is available as an option for
users who prefer to use this method for white light rather
than a visible light conversion screen. Various sizes are
available to suit different
sample sizes.
(See website for further
information).
LED modules
Edge light unit
For users wishing to work with Cy dyes in multiplex
applications, (eg DIGE), then a novel Edge lighting unit is
available. This unit uses high intensity LED lighting and
fibre optics to illuminate gels
for the edge. Light is ‘passed’
through the gel creating
fluorescence. The narrow
band nature of the
lighting enables red,
green and blue
channels to be
captured and viewed
with minimal ‘cross-talk’.
For Chemi XT4, XX6 and
XX9 only.
Plug and play modules which have either red, green or
blue LEDs and a corresponding excitation filter, provide
a high intensity lighting option for use with an extensive
range of coloured fluorophores. More specific ‘M’
versions of the modules are available for use with
multiplexed applications.
IR versions of the modules
are also available for
imaging LI-COR® IRdyes
and other IR Dye
alternatives. The IR
Multiplexing kit gives
you all the lighting and
filters required for this
application. Modules
clip into integral gantries
fitted on each side of the darkroom.
This enables the use of up to 8 modules (normally
modules are used as pairs - 1 per side).
(Not available on G:BOX F3).
(See website for further information).
15
G:BOX FEATURES AND BENEFITS
Why you should be choosing a G:BOX for your work
Features
Benefits
New generation very high
Superior sensitivity – allows capture of very low
quantum efficiency cameras
light emissions from gels and blots
Fully automatic control
Effortless capture of chemiluminescent and
multiplex images without ‘guesswork’
Wide range of lighting options
Fully programmable to work with any application
including complex multiplex experiments
16
G:BOX SPECIFICATIONS
Choose your G:BOX
G:BOX F3
G:BOX Chemi
XRQ
G:BOX Chemi
XT4
G:BOX Chemi
XX6
G:BOX Chemi
XX9
3.8
15.3
12/16 bit
65536
52%
N/A
f1.2
Yes
Yes
Yes
4
16
16 bit
65536
73%
-57C
f1.2 with
feed back
Yes
Yes
Yes
4.2
16.76
16 bit
65536
53%
-57C
f0.95 with
auto focus
Yes
Yes
Yes
6
18
16 bit
65536
73%
-57C
f0.95 with
auto focus
Yes
Yes
Yes
9
27
16 bit
65536
73%
-57C
f0.95 with
auto focus
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Optional
Optional
Optional
Optional
Optional
Yes
Optional
Optional
Optional
Optional
Optional
Yes
Optional
Optional
Optional
Optional
Optional
Yes
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
Optional
25.5 x 21
4.5 x 3.8
57 x 84 x 45
37
115v/240v
30.5 x 22.7
5.5 x 4
57 x 84 x 45
37
115v/240v
19 x 19
9x9
57 x 99 x 55
45
115v/240v
32.3 x 25.6
15 x 11.8
57 x 99 x 55
45
115v/240v
32.3 x 25.6
15 x 11.8
57 x 99 x 55
45
115v/240v
System
Image resolution (pixels m)
Effective resolution (pixels m)
A/D
Greyscales
Quantium Effiency @ 425nm
Cooling - regulated
Lens (Motor driven)
Filter wheel (7 position motor driven)
UV Filter**
Use with external PC *
Darkroom
Standard
Extended with motor driven stage
Illumination
Epi LED White lights
EPI UV 302nm****
EPI Red LED module
EPI Blue LED Module
EPI Green LED Module
EPI Red LED module M series
for multiplexing
EPI Green LED Module M series
for multiplexing
EPI Blue LED Module M series
for multiplexing
EPI IR LED module
IR Multiplexing kit (680 - 800nm)
Visible light converter 33 x 31cm
White light pad for visible stains
(20 x 14 or 20 x 30cm*****)
UltraBright LED Blue light
transilluminator 20 x 16cm
Edge lighting unit 26.5 x 20cm
UV Transilluminator
302nm 20 x 20cm***
Yes
Optional
Dimensions
Max image area (cm)
Min image area (cm)
w x h x d (cm)
Weight (kg)
Voltage
*See web site for current specifications
**See list of other available emission filters
*** Other sizes and wavelengths available including dual wavelength
**** Other wavelengths available
***** For G:BOX F3 & Chemi XRQ only
We reserve the right to alter specification without prior notice.
17
Over 75,000 scientists world-wide
in pharmaceutical and biotech
companies, as well as academic
and government institutions, have
chosen Syngene as their expert
imaging partner. If you’d like to
find out why, please contact us or
one of our dealers for more
information and a demonstration
of the revolutionary G:BOX
Syngene Europe and
International Headquarters:
Beacon House Nuffield Road
Cambridge CB4 1TF UK
Tel: +44 (0)1223 727123
Fax: +44 (0)1223 727101
email: sales@syngene.com
Syngene USA Headquarters:
5103 Pegasus Court Suite L
Frederick MD 21704 USA
Tel: 800-686-4407/301-662-2863
Fax: 301-631-3977
email: ussales@syngene.com
Website: www.syngene.com
Please refer to
www.syngene.com
for all ordering
information
A.055.02.16
All trademarks acknowledged
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