Leica ST5020 Instructions for Use, V 2.0, RevG

Leica ST5020 Instructions for Use, V 2.0, RevG
Instructions for Use
Leica ST5020
Multistainer
Leica ST5020, V 2.0 RevG, English – 07/2016
Order No.: 14 0475 80101 RevG
Always keep these instructions near the instrument!
Read carefully before working with the instrument.
NOTE
The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge
and state-of-the-art technology as we understand it following thorough investigation in this
field.
We are under no obligation to update the present manual according to the latest technical
developments, nor to provide our customers
with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according to the national legal system applicable in
each individual case. In particular, no liability
whatsoever is accepted for any financial loss or
consequential damage caused by or related to
compliance with statements or other information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical details of the present manual are not to be
considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is
it possible to continuously improve the technology and manufacturing techniques used in our
products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods –
including any electronic systems and media –
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (0)62 24 143-0
Fax:
+49 (0)62 24 143-2 68
Internet:http://www.LeicaBiosystems.com
Leica ST5020
3
Table of Contents
1.
Important information................................................................................................................ 6
1.1Symbols used in this manual and their meaning.............................................................................. 6
1.2 Designated use...................................................................................................................................... 7
1.3 Qualification of personnel.................................................................................................................... 7
1.4 Instrument type...................................................................................................................................... 7
2.Safety............................................................................................................................................ 8
2.1 Safety instructions................................................................................................................................ 8
2.2Warnings................................................................................................................................................. 8
3.
Instrument components and specifications........................................................................ 11
4.
Setting up the instrument........................................................................................................ 18
3.1
3.2
3.3
3.4
3.5
Overview - Instrument components................................................................................................. 11
Instrument specifications.................................................................................................................. 13
Standard delivery - packing list........................................................................................................ 14
Technical Data..................................................................................................................................... 15
Container system................................................................................................................................. 17
4.1 Installation site requirements............................................................................................................ 18
4.2 Installing the instrument..................................................................................................................... 18
4.3 Tap water supply connection............................................................................................................ 19
4.4 Installing the oven (optional)............................................................................................................. 22
4.5 Connecting the air evacuation hose (optional).............................................................................. 25
4.6 Inserting the activated carbon filter................................................................................................. 26
4.7 Leveling the instrument...................................................................................................................... 26
4.8 Electrical connection.......................................................................................................................... 28
4.9 Alarm functions.................................................................................................................................... 29
4.10Transport............................................................................................................................................... 29
5.Operation.................................................................................................................................... 30
5.1 Switching the instrument on.............................................................................................................. 30
5.2 Setup checklist.................................................................................................................................... 31
5.3 Touch screen functions...................................................................................................................... 32
5.3.1 User interface - overview.................................................................................................................. 32
5.3.2 Data entry............................................................................................................................................. 35
5.3.3 Access levels....................................................................................................................................... 37
5.3.4 The main menu..................................................................................................................................... 38
5.4 System SETUP...................................................................................................................................... 39
5.4.1 User interface...................................................................................................................................... 40
5.4.2Alarm..................................................................................................................................................... 41
5.4.3 Password allocation........................................................................................................................... 43
4
Instructions for Use, V2.0, RevG - 07/2016
Table of Contents
5.4.4 Set date/time........................................................................................................................................ 44
5.4.5Movement............................................................................................................................................. 45
5.4.6 View and print...................................................................................................................................... 46
5.4.7 Reagent list........................................................................................................................................... 47
5.4.8 Station options..................................................................................................................................... 50
5.4.9 Calibration dialog................................................................................................................................. 51
5.4.10Data backup.......................................................................................................................................... 52
5.5 Instrument status................................................................................................................................. 54
5.5.1 Station buttons..................................................................................................................................... 55
5.5.2 Station types......................................................................................................................................... 55
5.5.3 Select station type............................................................................................................................... 57
5.5.4 Change station type ........................................................................................................................... 59
5.6 Staining programs............................................................................................................................... 60
5.6.1 Creating programs............................................................................................................................... 61
5.6.2 Defining the program steps............................................................................................................... 62
5.6.3 Assigning a clip color......................................................................................................................... 67
5.7 Programs in process........................................................................................................................... 69
5.8Staining................................................................................................................................................. 70
5.8.1 Loading the racks................................................................................................................................ 70
5.8.2 Starting programs................................................................................................................................ 71
5.8.3 Interrupting a staining program........................................................................................................ 73
5.8.4 Finishing programs.............................................................................................................................. 74
6.
Cleaning and maintenance..................................................................................................... 76
6.1
6.2
6.3
Cleaning the instrument..................................................................................................................... 76
General maintenance......................................................................................................................... 77
Preventative maintenance schedule............................................................................................... 78
7.Troubleshooting........................................................................................................................ 79
7.1General.................................................................................................................................................. 79
7.2 Correcting errors................................................................................................................................. 79
7.3 Power failure........................................................................................................................................ 82
7.4 Replacing the secondary fuses......................................................................................................... 83
8. Warranty and service............................................................................................................... 85
9.Appendix.................................................................................................................................... 86
9.1
9.2
Leica ST5020
Container Map..................................................................................................................................... 86
Optional accessories.......................................................................................................................... 88
5
1. Important information
1.1Symbols used in this manual and their
meaning
Warnings
appear in a grey box and are marked by
a warning triangle
.
Notes,
i.e. important user information appear
in a grey box and are marked by an information symbol
.
Flammable solvents and reagents are
marked with this symbol.
Instrument surfaces which become hot
during operation are marked with this
symbol.
(5)
ENTER
Figures in brackets refer to item nos. in
drawings.
Function keys to be pressed on the instrument touch screen are written in
bold-print capital letters.
The CSA test mark means that a product has been tested and fulfills the applicable safety and/or performance
standards, including the relevant standards defined or administered by the
American National Standards Institute
(ANSI), Underwriters Laboratories (UL),
the Canadian Standards Association
(CSA), the National Sanitation Foundation International (NSF) and others.
In vitro diagnostics (IVD) medical device.
Observe the Instructions for Use!
Environmental protection symbol of the
China RoHS directive. The number in
the symbol indicates the "Environmentfriendly Use Period" of the product in
years. The symbol is used if a substance restricted in China is used in excess of the maximum permitted limit.
Symbol for labeling electrical and electronic equipment in accordance with
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG).
ElektroG is the law regarding the sale,
return and environmentally sound disposal of electrical and electronic equipment.
Indicates the need for the user to consult the instructions for use for important cautionary information such as
warnings and precautions that cannot,
for a variety of reasons, be presented
on the medical device itself.
This product fulfills the requirements of
the Council's Directive 98/79/EC concerning in vitro diagnostics (IVD) medical devices.
Designates the serial number of the instrument.
6
Instructions for Use, V2.0, RevG - 07/2016
1. Important information
Order number for standard delivery or
accessories.
Date of Manufacture: Indicates the date
when the medical device was manufactured.
• The instrument may be operated only according to the instructions contained in this
manual.
Any other use of the instrument
is considered improper!
1.3 Qualification of personnel
Manufacturer: Indicates the manufacturer of the medical product.
1.2 Designated use
The Stainer Leica ST5020 is an automated stainer for the preparation of histological and cytological routine stainings.
It is designed for use in pathology laboratories,
and only for performing the following tasks:
• Staining of thin sections of tissue specimens
or of cytological samples, attached to microscope slides
• The instrument may be operated only according to the instructions contained in this
manual.
Any other use of the instrument
is considered improper!
• The Leica ST5020 may be operated only by
trained laboratory personnel.
• The instrument may be operated only according to the instructions contained in this
manual.
1.4 Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
A name plate indicating the instrument serial
number is attached to the back of the instrument.
To prevent damages to the instrument
and specimens, only those accessories and spare parts which have been
authorized by Leica may be installed or
used with the instrument.
Leica ST5020
7
2.Safety
Make sure to comply with the safety instructions and warnings in this chapter.
Make sure to read these instructions, even if you are already familiar with the operation and
use of other Leica products.
2.1 Safety instructions
This Instructions for Use includes important information related to the operating safety and
maintenance of the instrument and it is an important part of the product.
If additional requirements on accident
prevention and environmental protection exist in the country of operation,
this Instructions for Use must be supplemented by appropriate instructions
to ensure compliance with such requirements.
This instrument has been built and tested in accordance with the following safety regulations
on electrical measuring, control, regulating and
laboratory devices.
In order to maintain this condition and to ensure safe operation, the operator must observe
the instructions and warnings contained in this
Instructions for Use.
For current information about applicable standards, please refer to the CE
declaration of conformity on our Internet site:
www.LeicaBiosystems.com
Material safety data sheets can be obtained from the supplier of the chemicals.
They are also available on the Internet:
http://www.msdsonline.com
The protective devices on both instrument and accessories may neither be removed nor modified. Only authorized and qualified service personnel may repair the instrument and access the
instrument's internal components.
2.2Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Primarily responsible for accident-free operation is above all the institution which owns the instrument and, in addition, the designated personnel who operates,
services or repairs the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
8
Instructions for Use, V2.0, RevG - 07/2016
2. Safety
Warnings - Markings on the instrument itself
Markings on the instrument showing the warning triangle indicate that the correct Instructions for Use (as defined in this manual) must be followed when operating or replacing the item
marked.
Failure to adhere to these instructions may result in an accident, personal injury, damage to the
instrument or accessory equipment.
Some instrument surfaces, which become hot during operation are marked with this
warning label. Touching these surfaces may cause burns.
Warnings - Transport and Installation
The instrument may only be transported in an upright position.
Four people are needed to lift / carry the instrument.
Install the instrument on an even laboratory bench which must be absolutely level.
Do not expose the instrument to direct sunlight (windows).
The instrument MUST be connected to an earthed mains power outlet socket. The instrument
must not be connected to an extension cord without protective earth conductor.
The instrument will automatically adjust to the required voltage and frequency at the place of
installation.
If new ovens are installed, they must be adjusted to the voltage and frequency required at the
installation site.
The instrument must be set up in a well ventilated area, free from any ignition sources. The
chemicals to be used in the Leica ST5020 are both flammable and noxious. Do not operate the
instrument in rooms with explosion hazard.
If there is a significant difference in temperature between the warehousing and the installation
site of the instrument and if at the same time there is a high air humidity level, condensation
water may form. In this case, a waiting period of at least two hours must be observed before the
instrument is switched on. Failure to adhere to this waiting period may result in damage to the
instrument.
Warnings -handling reagents
Be careful when handling solvents!
Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument.
Reagents used for tissue infiltration can be both toxic and/or flammable.
Dispose of waste solvents with care according to local regulations and the waste management
policy of the company or institution.
Leica ST5020
9
2.Safety
Warnings - Operating the instrument
The Leica ST5020 may only be operated by trained laboratory personnel, according to its designated use and per the present Instructions for Use.
In case of emergency switch off mains and unplug the power chord.
While working with reagents (filling / emptying the reagent stations, working on the instrument
while the lid/s is/are open) appropriate protective gear (lab coat, gloves, safety goggles) must
be worn.
Make sure to operate the instrument either with the activated carbon filter or with the exhaust
air hose. Even when the instrument is operated according to its designated use, hazardous solvent fumes develop, which are damaging to the operator's health and do also pose a risk of fire!
Risk of fire, when working with an open flame (Bunsen burner) immediately next to the instrument (solvent fumes)! - Therefore, keep a safety distance of 2 meters!
If a staining program is to be interrupted for an extended period of time, do not leave any slide
racks in the tap water stations, in order to prevent them from drying out.
Warnings - Cleaning and maintenance
Prior to each maintenance and/or cleaning, switch the instrument off and disconnect mains
power. Do not clean the instrument with solvents containing acetone or xylene. No liquid may
be spilled into the internal components of the instrument - neither during operation nor during
cleaning.
When working with cleaning detergents, comply with all safety instructions by the manufacturer of the product and the laboratory management policy.
Wash the tap water and reagent stations in the dishwasher at a temperature of max. +65 °C
(149 °F). Use a standard detergent for laboratory dishwashers.
At any rate avoid washing the stations at higher temperatures, as the stations may become deformed!
Spilled solvents (reagents) have to be wiped away immediately! - In case of long-term exposure, the lid surfaces are only conditionally resistant to solvents!
To clean the lids, control panel and housing, use mild household detergents; - see safety instruction above for non-appropriate ingredients!
Caution with sequences which involve an oven at the initial step.
In this case the loading unit, out of which the specimen holder is removed via the transport arm,
must NOT be filled with an inflammable reagent (e.g. xylene).
As the oven temperature is up to 80 °C (176 °F), the reagent may ignite and cause damage to the
device and to the samples.
For the same reason, steps conducted INSIDE an oven must never be carried out from a reagent
station with inflammable reagents.
10
Instructions for Use, V2.0, RevG - 07/2016
3. Instrument components and specifications
3.1 Overview - Instrument components
Activated
carbon filter
Transfer arm
Single (Double)
carrier
Oven
modules
Lid
Wash
stations
Reagent
container
Color touch
screen
Mains
switch
Transfer to
Coverslipper
Leica CV5030
(optional)
Exit
drawer
Load
drawer
Slot for
PCMCIA
card
Fig. 1
Leica ST5020
11
3. Instrument components and specifications
Rear of instrument
Fan
electronic
Data
connections
Power supply
inlet
(electronic)
Power supply
outlet
Mains
Power supply
inlet
Transfer to
Coverslipper
(optional)
Secondary
fuses
adjustable
foot
Cover for
Oven-Modules
Drain
hose
Exhaust
air duct
Drain
outlet
DI-Water inlet Water inlet
Station 7
Stations 8-12
Fig. 2
Container map
Oven-Modules
Wash
stations
Wash
station
DI-water
Reagent
container
1
2
3
4
6
7
8
13 14
15
16 17 18
19
20 21
22 23 24
25 26 27
28 29 30
31 32 33
34 35 36
Exit drawer
5
9
10
11 12
37
38 39 40
Color touch
screen
Load drawer
Fig. 3
12
Instructions for Use, V2.0, RevG - 07/2016
3. Instrument components and specifications
3.2
Instrument specifications
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
High specimen throughput (at maximum 12 racks simultaneously)
Simultaneously processing of multiple different programs
Color TFT-Touch screen
Graphical and intelligent user Interface
Context-sensitive Online Help
Graphical process representation on color screen.
Reagent Management System.
Multilingual.
Maximum of 40 stations.
Load drawer with max. 4 stations.
Exit drawer with max. 4 stations.
Maximum of 6 wash stations, 2 different water sources possible.
(individually valve-controlled)
CodeRackTM Programmable Slide Rack for automatic rack start.
Overflow Protector (sensor) in stainless steel sink.
Integrated fume control system with external hose/filter.
Optional Accessories
• Oven modules max. +65 °C and up to 4 stations.
• Heated reagent stations (35 - 70 °C max.), max. 2,
• Special Stain Kit.
(modified container with reduced reagent volume and racks).
• System upgrade for Coverslipper integration.
(automated staining and coverslipping without operator interaction).
• Air evacuation hose.
• Slide rack (plastic).
• Adapter for racks from other manufacturers.
To prevent damage to the instrument or the specimen, only accessories authorized by Leica may
be used.
Leica ST5020
13
3. Instrument components and specifications
3.3 Standard delivery - packing list
The Leica ST5020 standard delivery consists of the following items:
1 Leica ST5020 basic instrument
31 Reagent vessels, assy. (with handles and lids)
5 Wash vessels, assy.
2 Activated carbon filter
1 Accessory-Kit consisting off:
1 Jumper cable - mains
3 Reagent vessels, assy. (with handles and lids)
1 Set of clips, red, pack of 5
1 Set of clips, yellow, pack of 5
1 Set of clips, white, pack of 5
1 Set of clips, light blue, pack of 5
1 Set of slide racks 30, plastic, pack of 5
1 Waste water hose, 4 m length
1 Hose clamp for waste water hose
1 Tap water inlet hose, 2.50 m length, assy. with 3/4" connection for water tap
1 'V' strainer 3/4"-40/22 A6 (strainer for inlet hose)
1 Threaded nippel 2 Gaskets for tap water inlet hose
1 Box level
1 Special O-ring grease for valves and O-rings (OHA), 25 ml
1 PCMCIA Flash Card, with adapter
1 Tool set:
1 Screw driver 5.5 x 200
1 Allen key, size 3.0, with handle
1 Allen key, size 4.0, with handle
1 Allen key, size 6.0
1 Ring-head wrench, size 13
1 Single-head wrench, size 17
1 Set of replacement fuses:
4 x T 2.0 A
2 x T 2.5 A
2 x T 4.0 A
1 Instructions for Use, printed (German/English, with language CD
14 0475 80200)
14 0475 33659
14 0475 33660
14 0474 32273
14 0475 39617
14 0411 34604
14 0475 33659
14 0475 33637
14 0475 33633
14 0475 33632
14 0475 33634
14 0475 33750
14 0475 35748
14 0422 31972
14 0475 32325
14 0456 36101
14 0474 33063
14 0300 00132
14 0475 37629
14 0336 36657
14 0475 42526
14 0170 10702
14 0194 04764
14 0194 04782
14 0194 03959
14 0330 37628
14 0330 19641
14 6943 02001
14 6943 02501
14 6943 04001
14 0475 80001
The country specific power cord needs to be ordered separately. Please find a list of all power cords
available for your device on our website www.LeicaBiosystems.com within the product section.
14
Instructions for Use, V2.0, RevG - 07/2016
3. Instrument components and specifications
3.4 Technical Data
General
Approvals:The instrument-specific marks are located next to the
identification label
Nominal voltage:
100 V - 120 V +/- 10 %
230 V - 240 V +/- 10 %
Nominal frequency:
50 to 60 Hz
Maximum power draw:
1400 VA
IEC 1010 classifications:
Protective class 1
Pollution degree 2
Overvoltage installation category II:
•
800 V impulse (120 V systems)
•
1500 V impulse (240 V systems)
Remote alarm relay:
24 V AC/DC, maximum 2 A
Connection:
potential-free contact
(can be operated both as normally open and as normally
closed circuit)
Primary fuses:
Fa. Schurter: type FST, mains power inlet 2 x T 8 A
Secondary fuses:
Melting fuse, Ø 6.3 x 32 mm
Type: Schurter FST
F1: T 2.0 A
F2: T 2.0 A
F3: T 2.5 A
F4: T 4.0 A
Operating temperature range:
Relative humidity:
15 °C to 40 °C
10 % to 80 %, non-condensing
Leica ST5020
15
3. Instrument components and specifications
Dimensions and weight
Basic instrument (W x D x H), in mm:
Dry weight, unpacked:
Weight, packed:
1060 x 750 x 540
approx. 90 kg
approx. 110 kg
Capacity data
Specimen slide throughput:
Loading capacity:
Slide rack capacity:
Total number of stations:
Total number of reagent stations:
Reagent container volume:
Number of wash stations:
Oven (optional):
Oven chamber temperature:
Incubation time setting:
Load/unload stations:
Permanent memory capacity:
Integration:
depending on the program-structure
max. 12 slide racks (with 12 different Programs)
30 specimen slides
40 (36 + 4 Oven)
max. 34
450 ml
max. 5 and 1 DI-water (optional)
2 or 4
40 °C to 70 °C or 104 °F to 158 °F
0 sec. up to 23 hours, 59 min., 59 sec.
max. each 4, min. each 1
max. 50 Programs, up to 40 program steps each
connection to Leica CV5030 Coverslipper (optional)
16
Instructions for Use, V2.0, RevG - 07/2016
3. Instrument components and specifications
3.5 Container system
6
1
3
Reagent container
The Reagent containers can be individually removed for filling.
For use, fill the reagent containers to the line marked on the inside (approx. 450 ml capacity) and place into position in the instrument consistent
with the programs you wish to run.
Ensure that the reagent containers are correctly seated and that the handles (1) are over to the side and will not obstruct slide rack movement.
Lids (6) are provided to reduce evaporation while the reagent containers
are not in use.
The Load and Exit drawer containers (E25 and L36) can be filled with a
reagent if desired.
However, the instrument will not control the immersion time in these stations.
Wash stations
The wash system consists of five (optional six) wash stations each capable of holding one slide rack. Water enters the wash station through a
connection piece (2) in the base and exits from the overflow lip (3) at the
top left hand edge.
Wash stations have a locating pin (4) and can only be inserted
one way. Take care when fitting or removing wash stations as
excessive force may damage the O-ring (5) used as a seal.
Lubricate the O-ring seal before fitting a wash station.
To use the wash system, slowly turn the laboratory tap on fully. The flow
control valve in the Leica ST5020 will limit the total water flow in the wash
stations to 1.6 liters/minute/station.
If the water flow drops below this level for any reason the wash
period specified in the program may have to be extended.
4
2
Leica ST5020
5
Fig. 4
17
4. Setting up the instrument
4.1 Installation site requirements
• Stable, exactly horizontal laboratory bench
with even surface of a minimum width of
1.60 m and minimum depth of 0.80 m.
• Tap water supply located a maximum of
2.50 m and waste water drain pipe a maximum of 2.00 m away from the corresponding in-/outlets at the rear panel of the instrument.
• If the instrument is to be operated with air
evacuation hose, a fume cupboard at a distance of maximum 3.50 m from the instrument is required.
Alternative: operation with activated carbon
filter.
• Sufficient space (approx. 0.90 m) above the
laboratory bench for opening / closing the
instrument lid without any problem.
• Virtually vibration-free floor.
• Stable ambient temperature between +10 °C
and +40 °C.
• Relative air humidity of maximum 80 %, noncondensing.
• Do not place near instruments which might
be sources of vibration.
4.2 Installing the instrument
• To lift, grip the instrument by the carrying
handles (1, Fig. 5).
The chemicals to be used in the
Leica ST5020 are both flammable and
noxious. The instrument must be set
up in a well ventilated area, free from
any ignition sources.
Do not operate the instrument in
rooms with explosion hazard.
Four people are required to lift and/
or carry the instrument, as the instrument weighs a total of approx. 95 kg.
2
1
Fig. 5
18
• Place the instrument onto the bench.
• Unscrew the carrying handles (remove
screws (2) with Allen key, size 6.0) and store
away.
• Pull off plastic cover.
• Check whether all accessories have been
delivered as per your order.
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
4.3 Tap water supply connection
Water filter
In addition to the filter sieve supplied (item 3 in Fig. 6) Leica recommends installing a sediment filter between the water extraction outlet
and ST5020, to guarantee that the instrument is only operated with clean
water.
This sediment filter should have the following properties:
Filter fineness:
25 µm
Rate of flow:
approx. 10 l/min (with 5 wash stations)
Pressure constancy:
10 bar
Connection to the filter output: 1/2" external thread
This filter insert must be checked and cleaned regularly.
If the filter insert is very dirty it must be replaced.
Connecting the tap water inlet hose
• Unpack the tap water inlet hose - mind the two gaskets (4).
7
5
3
4
6
7
Fig. 6
Leica ST5020
• Insert one gasket (4) into threaded fitting (6). Store away the remaining
gasket(s).
• Insert strainer (3) into union nut (5) (see Fig. 6) which is screwed onto
the water tap. Do not insert any further gaskets, as the strainer acts
also as a seal.
19
4. Setting up the instrument
• Screw tap water inlet hose (7 in Fig. 8) for
tap water stations 8 - 12 onto water tap and
insert the other end into inlet (8 in Fig. 7).
Instrument rear panel
- tap water inlets
8
9
10
• If station no. 7 (Fig. 3) will also be used as
a tap water station, connect a second tap
water inlet hose (optional accessory) to inlet (9).
Fig. 7
Inlet (9) only provides water (or any
other medium supplied via a separate connection) to station no. 7. To use
station 7 as a water station, a wash
vessel must be inserted and station 7
must be defined as a 'tap water' or
'fully-de-ionized water' station (DI
water station) in 'stations options'.
(see also chapter 5.5.3)
Instrument rear panel
water in-/outlets
7
10
12
11
Fig. 8
20
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
Connecting the waste water hose
• Outlet connection piece (10) can be turned
to point into different directions, depending
on whether the drain water pipe is located
to the left or to the right of the instrument.
7
13
• To adjust the connection piece, loosen
screw (11) of hose clamp (12) sufficiently so
that the connection piece (10) can be turned. Adjust the connection piece as desired
(always with the opening pointing slightly
downwards) and retighten screw (11).
15
• Turn screw (15) to the left (use screwdriver)
until hose clamp (14) opens wide enough to
be slid over waste water hose (13).
14
Fig. 9
Important!
When installing the drain hose always make sure there is a sufficient
descending gradient from the outlet
(connection piece (10)) on the instrument to the drain pipe!
Leica ST5020
• Fit waste water hose (13) onto outlet connection piece (10) and position hose clamp
(14) as shown in Fig. 9.
• Turn screw (15) to the right until hose (13) is
secured tightly on connection piece (10) by
hose clamp (12).
• Insert waste water hose (13) into drain pipe
and secure in position.
21
4. Setting up the instrument
• Take the oven kit out of the box and verify
whether it is complete.
4.4 Installing the oven (optional)
15
The following parts must be included:
20
19
21
15 - Oven module
16 - Paraffin collecting tray
17 - Cover
18 - Screws
19 - Washers
20 - Screwdriver
21 - Allen key, size 2.5
22 - Allen key, size 4.0
18
22
17
Insert paraffin collecting tray
into corresponding opening in
oven module (possible only in
one direction, as shown here).
16
Fig. 10
Voltage setting
100 - 120 V
Rear panel - oven
• Check the voltage selector setting and adjust if necessary.
Important!
The voltage selector switch must be
set to the same mains voltage as the
instrument.
23
24
25
Voltage setting
230 - 240 V
To adjust the voltage selector setting, insert
screw driver (20) into gap (23) and turn carefully until the white point above the correct
voltage indication is located underneath the
white triangle.
115 means: Instrument voltage 100 -120 V.
230 means: Instrument voltage 230 - 240 V.
Fig. 11
22
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
Important!
Prior to taking any further installation
steps, switch the instrument off and
unplug from mains.
27
• Cover panel (26) must be removed from the
right inner wall of the instrument, so that the
transfer arm can access the oven stations.
26
27
• To do so, remove six screws (27) (Allen key,
size 2.5) and remove cover panel (26).
• Store screws and cover panel in safe place
for further use.
Fig. 12
29
29
28
29
• Remove cover panel (28) located to the right
of the electrical connections at the rear wall
of the instrument.
30
• To remove panel (28), only remove screws
(29).
Do not loosen the two screws (30)!
• Once the oven is installed, attach cover
panel (part of oven kit delivery) (17 in Fig. 10)
with four screws (29) in such a way that the
voltage selected is visible from the outside.
29
Fig. 13
Leica ST5020
23
4. Setting up the instrument
Installing the oven (continued)
Important!
If only one oven is installed, it must
be located on the right side (seen
from the rear of the instrument).
31
• Place oven on rails (32) and insert.
• Keep pushing until the connection pieces
of the oven (positioning pin (24), connector
strip (25), see Fig. 11) lock into the corresponding connections (31) in the instrument.
32
Fig. 14
• Next, use the two screws (18) and washers
(19) to secure the oven (15) in the bores provided in the instrument for that purpose.
15
• If a second oven has been ordered, install it
the same way as the first.
18, 19
• Fasten cover (item 17, Fig. 10) so that the
voltage range selected for the oven (15) is
the same as the engraved voltage range.
Fig. 15
• Plug the instrument back into mains and
switch on. The oven modules are detected
automatically and are displayed accordingly
in 'instrument status'.
INSTRUMENT STATUS
Indication prior to
installing the ovens
Indication with
ovens installed
Fig. 16
24
• Go to 'stations options' and set the desired
oven temperature.
(See chapter 5.5.3, page 56 for further details).
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
If the instrument is operated without activated
carbon filter, the air evacuation hose must be
installed instead.
The air evacuation hose is attached to fan
housing (68) located at the rear left of the instrument. Unpack the components supplied
and check for completeness. The package must
contain the following items:
60 - Screw driver
61 - Allen key, size 2.5
62 - Hose clamp
63 - Allen screw with washer
64 - Hose connection piece with seal
65 - Air evacuation hose
4.5 Connecting the air evacuation hose
(optional)
60
64
65
63
62
61
Fig. 17
66
65
64
68
67
63
62
66
Fig. 18
• Fasten connection piece (64) with two
screws (63) into tap holes (66) in fan housing
(68).
• Turn clamping screw (67) to the left (use
screw driver (60) until hose clamp (62)
is loosened far enough so that it can be
slipped over the air evacuation hose.
Leica ST5020
• Place air evacuation hose (65) together with
hose clamp (62) onto connection piece (64)
(see Fig. 18, right).
• Tighten screw (67) of hose clamp (62) (turn
to the right) to secure the hose.
25
4. Setting up the instrument
4.6 Inserting the activated carbon filter
51
34
Important for trouble-free operation:
The sealing profiles (33) must
fit against the rear wall of the
filter housing (34).
Filter - inserted
33
35
Fig. 18
Make sure to insert the filter
with the correct side pointing
upwards: the three arrows on
the label (51) located at the
right of the filter must point to
front.
Fig. 19
Once the activated carbon
filter is inserted, push lever
(35) downwards to properly
secure the filter in the filter
housing.
4.7 Leveling the instrument
• Once all accessories are installed (don't forget the rear panel!), move
the instrument to its final position.
• Prior to leveling the instrument, check whether the bench surface of
the laboratory bench where the instrument will be installed is flat and
exactly horizontal.
• Otherwise, use the box level (standard delivery) to determine the highest point of the bench. Next, the instrument foot located at the highest
point of the bench must be screwed in almost completely (see Fig. 21).
- Then level the instrument adjusting each foot as needed.
26
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
Adjustable
instrument feet
Bring the instrument
to an exact horizontal
position by screwing the
adjustable feet in / out as
needed.
To do so, proceed as follows:
• Remove all vessels located in stations 1 - 24
so that the vessel holder frames can be used
to support the box level for measuring purposes.
• All four instrument feet (37) are height-adjustable.
To adjust, place single head wrench, size 13
(standard delivery) onto hexagon bolt (36)
and adjust each foot as needed.
Make sure, all four instrument feet
are firmly set on the bench surface
once the instrument adjustment has
been completed.
36
37
Fig. 21
• For leveling, position box level (38) alternately in positions
(A and B) - see Fig. 22 on the
vessel holder frame.
B
C
39
39
• Screw the instrument feet
inward / outward as needed,
gradually bringing the instrument into a horizontal position. Start with the instrument
foot located opposite the
highest point of the laboratory bench.
A
D
38
Fig. 22
Fig. 22 shows all four points (A to D) where the box level
must be positioned for leveling the instrument.
For leveling, the box level must be placed onto vessel
holder frame (39). - Do not place the box level elsewhere.
Leica ST5020
• Finally, place the box level in
positions (C und D) to doublecheck whether the instrument is in fact in a horizontal
position.
27
4. Setting up the instrument
4.8 Electrical connection
The instrument MUST be connected to an earthed mains power outlet socket.
The instrument is supplied with a set of country-specific power cords.
Make sure to use the appropriate mains cable for the local voltage supply (wall outlet).
Sockets for mains power
supply and data transfer.
Secondary fuses.
50
Jumper cable / power cord
connected correctly for mains
power supply
49
47
48
44
43
45
42
All electrical connections are located at the rear
of the instrument to the left (Fig. 23).
Power supply
• Connect mains cable to mains power supply
socket (42).
• Jumper cable (43) connects the mains power
supply output (44) to the electronics module
input (45).
Data connections
• Printer port (47)
A suitable printer (and cable) can be recommended by your Leica distributor. If conformance to electromagnetic interference standards is essential, a specially shielded printer
cable will be required.
• Serial port (48)
Access for technical service.
• Connection for Leica CV 5030 Cover slipper
(49).
All interfaces may be used only with
devices that have been tested in accordance with EN 60950 and which satisfy the requirements for SELV circuits.
Other connections
• Socket for remote alarm system (50)
• Secondary fuses (46)
46
Fig. 23
28
Instructions for Use, V2.0, RevG - 07/2016
4. Setting up the instrument
4.9 Alarm functions
Instrument alarm
The instrument alarm is generated from within
the Leica ST5020.
The instrument alarm is used for all alarm situations.
Remote alarm
This alarm is external to the Leica ST 5020.
Any alarm generated in the instrument, no matter of which alarm type, is passed on to the
remote alarm system, even when the type 1 instrument alarm is switched off.
The remote alarm sound/volume is the same
for all alarm types - individual sound and volume settings selected in the ALARM menu (see
chapter 5.4.2) are disregarded.
Connecting a remote alarm device
The remote alarm system which can be optionally connected works with a potential-free relay. If an alarm is generated, the alarm circuit
closes.
The remote alarm device connected to the instrument must be rated at less than 2 amp. A
maximum voltage of 24 V AC/DC may be present.
The remote alarm device is connected to the
printer via a 6.3 mm -diameter jack connector
(optional accessory).
For details on how to connect a remote alarm
device to the Leica IP, please contact your local Leica sales office or the manufacturer Leica
Microsystems Nussloch GmbH directly.
4.10Transport
The following measures must be taken if the instrument is to be transported:
• Remove all the racks.
• Empty all reagent containers.
• Secure the transport arm of the instrument.
• Disconnect the instrument from the supply
voltage.
• Detach the water inlet hose and the water
drain hose.
• If necessary, detach the air evacuation
hose.
• Reattach the carrying handles (see chapter 4.2).
• If the instrument is transported over longer
distances, pack the instrument in its original shipping box and follow the measures for
securing it for transport.
Leica ST5020
Securing the transport arm:
• Change to the Supervisor mode.
• Call up the keyboard (e.g. as required for entering the password, see chapter 5.4.3)
• Enter the command "#transport".
The arm moves down into station 6 where it remains and can no longer be moved.
After switching the instrument off, it is ready for
transport.
29
5.Operation
5.1 Switching the instrument on
Caution!
The instrument MUST be connected to an earthed mains power outlet socket.
It is recommended that the Leica ST5020 be plugged into a wall socket that has Ground Fault
Circuit Interruption (GFCI) protection - as an additional electrical safeguard.
• Before you connect the instrument to the wall socket, make sure the
mains switch at the bottom right of the instrument is in position OFF ('0').
• Connect the mains cable to the wall socket and switch on the mains
supply at the wall socket (if applicable).
• Switch the mains switch ON ('I').
• When switched on, the instrument will take
a few seconds to initialize. During initialization the screen is blue, initialization progress
is indicated by a line of white dots.
• After approx. 30 seconds, the graphic surface will become visible. The working area
is still black. A row of "X" displays the software loading in the information line.
• Next, the LANGUAGE CHOICE menu (Fig. 24)
will be shown on the screen for approx.
three secs.
• If you select a different language (press the
corresponding button), the MAIN MENU
(Fig. 32, chapter 5.3.4) will be shown next, in
the new language selected.
• If no changes are made, the INSTRUMENT
STATUS menu (Fig. 25) will open up.
INSTRUMENT STATUS is the standard
screen - all functional descriptions in the
present chapter 5 start from INSTRUMENT
STATUS.
Fig.8
Fig. 24
30
Fig. 25
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.2 Setup checklist
Once the instrument is ready to be switched on, all menus listed below must be accessed and
the necessary parameters be properly selected. The Supervisor mode must be active to have
access to all menus. For detailed advice on how to select individual parameters, refer to the
online help feature.
Screen →
→ Press button
→
↓
Parameter settings
Select a language - either when switching the instrument on or in the LANGUAGE CHOICE menu.
In USER INTERFACE, set parameters as required.
→
→
Verify whether DATE and TIME are correctly set.
→
→
Open the REAGENT LIST menu, check the settings
and complete, if necessary.
→
In INSTRUMENT STATUS, select a STATION TYPE
for each station.
Allocate the correct reagent to each STATION.
Reagents can be allocated either when creating the
programs or in the STATION DETAILS menu.
Be sure to fill the reagent containers with the correct reagents (as allocated)!
↓
←
Create the PROGRAMS needed.
Existing programs can be copied and modified.
→
→
→
In MOVE, select the number of dips and the speed of
the transfer arm.
→
→
Allocate a CLIP COLOR to each clip.
→
→
Select time, quantity and paper format of the daily
printouts.
Leica ST5020
31
5.Operation
5.3 Touch screen functions
5.3.1User interface - overview
The Leica ST5020 Multistainer is programmed and operated via a colored TFT- touch screen.
Basic screen layout
Only those control elements that can actually be accessed by the user are displayed on the screen.
The number of accessible control elements depends
on the access level selected.
Current
date
Current
time
Instrument
name
Menu
currently
selected
Information
line
Access level
selected
The screen is
horizontally
divided into
three functional areas:
↓
Status bar
Operation
Button bar
Fig. 26
Up one
menu level
32
Area for buttons
displayed depending on current menu
Switching from
Up one level
to main menu user to supervisor
access level and
back
Online help on
current menu
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
Button symbols
The six buttons described below have the same function in all menus.
On the left side of the button bar (Fig. 26) there will either be the button
BACK →
To go back to the previous menu.
or the two buttons
CONFIRM →To exit the current menu (new entries will be stored
upon exiting).
CANCEL →To exit the current menu without storing any of the
modifications made.
PRINT
→Displayed in menus containing tables and lists.
If pressed, the list selected will be printed immediately. Immediate printing does not affect the daily
printout of all protocols (see chapter 5.4.6).
Enabled
Disabled
→
Leica ST5020
MENU →
HELP →The online HELP feature can be accessed from any
screen. HELP provides context-sensitive explanations, i.e. help topics displayed will vary depending
on the menu HELP is accessed from.
To go back to the main menu (see chapter 5.3.4)
Some button symbols may look differently depending on whether they
have been enabled or disabled.
Disabled button symbols appear in a lighter color than enabled ones.
They are only shown to complement the menu being displayed, in order to avoid mix-ups and to remind the user that the corresponding
function has been disabled. They can, however, not be accessed.
There are several functions where the user can choose from a number
of given parameters.
If there are only two parameters available, the parameter currently
enabled is shown on the button. Pressing the button switches to the
alternative parameter.
If there is a choice of more than two parameters, the range of available parameters is shown on the button in square brackets.
Each time the button is pressed, the next parameter is accessed, starting again with the first after the last one in the row has been selected.
33
5.Operation
Tables/lists
1
Fig. 27
2
The program contains numerous tables.
The first row of a table contains the headers of
the individual columns.
The rows below contain the actual content.
Selected rows appear in blue color (1).
When a tabled is accessed, the blue bar is always located in the first content row.
If a table is empty, no blue bar is displayed.
Headings of column where parameters can
be entered by the user (in supervisor mode)
(Fig. 28) are displayed as buttons (3).
In supervisor mode, some tables also contain a
number of additional buttons (2), depending on
the parameters selectable.
3
Fig. 28
The navigation buttons are located at the right border of the screen.
Each table contains two navigation buttons for row-by-row scrolling.
Each time one of the buttons is pressed, the highlighting bar is moved one
row up or down. You can also highlight rows by touching them directly.
For long lists, a set of page-up / page-down buttons will be displayed in
addition.
Pressing on of these buttons moves the highlighting bar one entire page
up or down; the last (first) row of the current page will be the first (last)
row of the new page. If there is no entire page left to the beginning (end)
of the table, the new page will be displayed starting with the first row
(ending with the last row) of the table with the highlighting bar jumping to
the first content row on the page.
Each table contains a PRINT button. If the button is pressed, the current
status will be printed out immediately.
34
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.3.2Data entry
Parameters and data can be set / entered several different ways:
Alphanumeric keyboard
4
5
Fig. 29
The alphanumeric keyboard
(Fig. 29) resembles a computer
keyboard. An entry field (5) and
instructions on what kind of data
to enter (4) are located above the
keyboard. The amount of characters that can be entered varies,
depending on the type of data to
be entered. If there is a predetermined number of characters to
be entered, that number will be
displayed in the entry field. The
cursor is located on the left.
The maximum number of characters to be entered may not be exceeded.
The following numbers are predefined:
Reagent names:
maximum 20 characters
Program names:
maximum 20 characters
Program abbreviation: maximum 3 characters
Passwords:
minimum 4 characters, maximum 20 characters.
The following buttons have special functions:
If pressed, button remains locked, all letters will be displayed in capitals. After any button has been pressed, SHIFT is released and the keyboard
will return to small letters.
If pressed, button remains locked - all letters will be written in capitals.
Press SHIFT LOCK once more to return to small letters.
Press this button to display special characters, above all vowels carrying
accents. These can also be displayed in capitals by pressing SHIFT/SHIFT
LOCK. After a special character has been selected, the keyboard automatically reverts to standard indication.
Leica ST5020
35
5.Operation
Navigation buttons
The navigation buttons have the same function in both keyboard types
(numerical, alphanumeric):
• Cursor jumps to the left / right border of the entry field.
• Cursor is moved one character to the left / right.
• Character on the left of the cursor is deleted.
Numerical keyboard
6
• Entry field located above the numeric keypad (left
aligned). Instructions on which parameter to be entered (6) are displayed above the entry field. Entry
position is marked by cursor, default parameters
will be stored. Pressing a numerical key deletes default parameters.
• If a figure entered exceeds or falls short of a limit setting, that limit setting will be displayed when
CONFIRM in the menu bar is pressed and the entry
field will not be exited.
• Depending on the type of entry to be made, only a
limited number of numeric characters can be entered.
• Press CONFIRM or CANCEL to exit the numerical
keypad.
Fig. 30
Press CONFIRM to store entries and go back to the previous menu.
Press CANCEL to go back to the previous menu without storing any new
entries.
36
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
The Leica ST5020 may be configured to allow
two level of user access.
User access level
• Users may run programs and view results.
On this level, the USER symbol is displayed
in the upper right corner of the touch screen.
5.3.3Access levels
Administrator symbol
User symbol
Fig. 31
→
In SUPERVISOR mode, NO programs
can be run. Switch to USER mode to
start a program.
You can only go back to SUPERVISOR mode (e.g. to select / change
settings) once all running programs
have been finished.
Leica ST5020
Supervisor access level
• Administrators may perform all user functions, and additionally create programs and
perform the instrument setup functions.
• To access supervisor level, press SUPERVISOR: the keyboard will be displayed. If a
password has been allocated, the keyboard
will appear on the screen.
(See chapter 5.4.3 for details on password
allocation).
Enter the 'Admin' password and confirm.
The SUPERVISOR symbol will then be displayed instead of the USER symbol and the
SUPERVISOR button will be replaced by the
USER button.
The Leica ST5020 may be configured
so that all functions are accessible to
all staff - effectively the instrument is
in Supervisor access level the whole
time. See chapter 5.4 "System setup".
37
5.Operation
5.3.4The main menu
After switching the instrument on, the main menu will be displayed only if the language is
changed. INSTRUMENT STATUS is actually the standard screen.
In all functional descriptions below, INSTRUMENT STATUS is assumed to be the standard
screen, all buttons shown start from INSTRUMENT STATUS.
↓
Opens up the main menu from where all program functions can be accessed.
The main menu can be accessed directly from almost all functions.
To be able to access
all necessary settings,
the instrument must be
operated in SUPERVISOR mode.
Fig. 32
From the main menu, four subordinate menus can be accessed:
• INSTRUMENT STATUSsee chapter 5.5
• PROGRAMS
see chapter 5.6
• STAINING SCHEDULEsee chapter 5.7
• OPTIONS
see chapter 5.4
38
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.4 System SETUP
In OPTIONS you find all submenus which
• determine what the user interface looks like:
- USER INTERFACE
- CLIP COLOUR
- DATE/TIME
• manage the reagents:
- REAGENT LIST
• control the print functions (optional)
- VIEW/PRINT
→
• determine the station type and allocate reagents:
- STATION OPTIONS
• define the dips / transfer speed:
- MOVE
• allow user data to be loaded and backed up
- BACKUP
All of the settings for the menus STATION OPTIONS, USER INTERFACE, REAGENT LIST and
VIEW/PRINT can be accessed in user mode. All other menus require administrator mode.
Determine speed
and number of dips
Set user-defined
system parameters
Set and control
stations
Calibrate screen
Define screen
displays and
printouts
Allocate and
control reagents
Detect color of
unknown clip and
allocate
Backup and read in
all user data
Set / control date
and time
Fig. 33
Leica ST5020
39
5.Operation
5.4.1User interface
→
→
In this screen you can select:
• BRIGHTNESS OF SCREEN
• TEMPERATURE MEASURING UNIT
• BUTTON CLICK
Two measuring units can be chosen from:
• The parameter selected is displayed on the
button, next to the "=" sign.
←→
Fig. 34
• Press the button to switch to the alternative
measuring unit.
↓
Fig. 35
40
Language selection
To select between the two languages that are
available, one of these two languages always
being 'English'.
The second language (default = German) next
to English is exchangeable.
A PCMCIA expansion card containing the desired language is needed.
Switch the instrument off and insert the
­P CMCIA card into the slot next to the mains
switch.
Switch the instrument back on, the new language will be automatically loaded.
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.4.2Alarm
→
→
→
All of the settings for the menus STATION OPTIONS, USER INTERFACE, REAGENT LIST and
VIEW/PRINT can be accessed in user mode. All other menus require administrator mode.
Alarm type 1:
Alarm 1 is generated when user intervention is
required.
Alarm type 2:
An error has occurred or user intervention is
urgently required.
Fig. 36
Alarm type 3:
A serious error has occurred.
Follow the instructions provided by the error
message.
If the same error occurs repeatedly, call Leica
Technical Service.
Different sound signals, in several intensity levels, can be allocated to
each alarm type.
ALARM TYPE X → SOUND Y
• Allocates one of four different sounds to the alarm type. Each time the
button is pressed, a different sound is allocated.
SOUND INTENSITY = Z
• Selects the intensity of the sound. For alarm type 1 settings from 0 to 4
are possible. Intensity level = 0: alarm sound switched off.
For alarm types 2 and 3 only intensity levels 3 and 4 can be set.
The level currently selected is indicated on the button.
TEST
• The alarm type set (sound, intensity) will sound for approx. 10 secs.
Leica ST5020
41
5.Operation
The table below shows the specific events each individual alarm type is allocated to.
Time/Location Alarm type
Event
Alarm ceases / switched off
Instrument
being
switched on:
Alarm 1
All unload containers occupied when instrument is
switched on.
- When all racks have been removed.
Alarm 3
Switching on after power failure while staining
procedure was in progress.
- When dialog box is acknowledged.
Drawers:
Alarm 3
Load/unload drawer open - time limit exceeded.
- After drawer has been closed.
Alarm 3
Load/unload drawer opened while gripper located in - After drawer has been closed.
drawer area.
Alarm 3
Unload drawer is full or rack was placed into the
unload drawer.
- After rack has been removed,
dialog box has been acknowledged.
Alarm 1
Rack bearing a clip of a color not allocated to any
program placed into load station.
- After rack has been removed or
program allocated.
Alarm 1
Rack bearing a white clip placed into load station.
- After rack has been removed or
program allocated.
Alarm 1
Rack bearing an unknown clip placed into load
station.
- After rack has been removed or
color allocated to unknown clip.
Alarm 3
Pause button has been pressed.
- When leaving pause mode.
Alarm 3
Unload drawer occupied, gripper attempting to
unload completed rack.
- After dialog box has been
acknowledged ('successfully
removed').
Alarm 3
A program the last step of which is not allocated to
the unload drawer has been completed. - Rack must
be removed.
- After dialog box has been
acknowledged ('successfully
removed').
Ovens:
Alarm 2
One of the ovens does not reach set temperature.
If the error occurs again, there will be a second
alarm after 60 seconds.
- After dialog box has been acknowledged
Malfunctions
requiring
service:
Alarm 3
Hardware failure, e.g. motion error of transfer arm.
If the error occurs again after the instrument has
been switched back on, the alarm will immediately
sound again.
Coverslipper:
Alarm 3
If the coverslipper requires an intervention by the
user to function or continue functioning.
- After dialog box has been acknowledged
Staining:
42
After dialog box has been acknowledged and instrument
switched off.
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.4.3Password allocation
→
→
→
Any changes in this menu require supervisor access level.
You may switch to supervisor mode only while no racks are being processed.
Switching password verification on/off
When switching between user and supervisor mode, switch the password
verification on or off:
• Press PASSWORD VERIFICATION.
• The alphanumeric keyboard opens up - enter the password.
• If the password is correct, the password verification button will be
switched from 'yes' to 'no' and vice versa.
Changing the supervisor password
• To change the password, the currently valid password must be entered first.
• Press ENTER, (keyboard opens up) and enter the current password.
• If an erroneous entry is made, an error message will be displayed
which must be acknowledged.
Attention!
• A distinction is made between capital and
small letters.
If the entry made is correct, the new password will have to entered twice. Each time,
press ENTER.
After the new password
has been entered twice, a
confirmation message will
be displayed.
• Fig. 37 shows all three command prompts
displayed when changing the password.
Fig. 37
Leica ST5020
43
5.Operation
5.4.4Set date/time
→
→
Many of the instrument functions are time-controlled. - therefore it is very important to set date
and time correctly.
Date
The DATE / TIME window displays the current
calendar month.
• To set the DAY press the corresponding button.
• Set MONTH and YEAR using the
arrow buttons.
• After having set the date, press
CONFIRM to exit.
Fig. 38
Time
• To set the time, press TIME - the
TIME window opens up.
• Use the arrow buttons to set
HOURS and MINUTES.
• As soon as you press CONFIRM
to store your entries, the clocks
starts running.
Fig. 39
44
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.4.5Movement
→
→
Any changes in this menu require administrator mode. For this reason, changes can only be performed if no staining programs are active.
The parameters set in MOVEMENT are valid for all programs.
For
• NUMBER OF DIPS
• DIP AMPLITUDE
• LIFTING SPEED
only the settings indicated on
the buttons can be selected.
The currently selected setting
shows on the button next to the
"=" symbol. Press the button to
go to the next higher setting.
Fig. 40
• NUMBER OF DIPS:
Indicates how often the gripper dips the
rack into a reagent container before the
rack is immersed in that container. 'Yes'
must be selected in the DIPS column of the
corresponding program.
No dips are performed if the setting
"Number of Dips = '0'" is used, even if
'Yes' is entered in the DIP column in
the program.
Leica ST5020
• DIP AMPLITUDE:
Defines the stroke length of the arm when
dipping the rack ('2' = longer stroke than '1').
• LIFT SPEED:
Indicates at what speed racks are removed
from reagent containers. The smaller the
numbers selected, the slower the speed. At
slower speeds, less reagent is carried over.
45
5.Operation
5.4.6View and print
→
→
To view all stored protocols. The
button bar of each protocol contains a PRINT button for individual
printouts of each protocol.
↓
Fig. 41
• Printing is possible only when a printer is
connected.
• Pressing the PRINT button starts printing the
selected list immediately. If printing is not
possible, the button is disabled.
• Press PRINT ALL to immediately print all six
protocols. Each protocol will start on a new
page.
Press PRINT OPTIONS to determine which protocols will be printed at what time.
• "Daily printout" can be switched OFF and ON
for each protocol (press the corresponding
'yes / no' button).
↓
If OFF is selected for all printable
protocols, no daily printout will be
made. This does not affect the immediate printout function.
Fig. 42
→
46
• Press PAPER FORMAT to select between
formats A4 and B4.
• Press DAILY PRINTOUT TIME to access the
daily printout time submenu. Select the daily
printout time as described above.
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.4.7Reagent list
→
→
The reagent list is an integral part of the user interface and can therefore be accessed from
several different menus. Adjust it to the current reagents as soon as possible, as this makes
programming considerably easier.
Fig. 43
• There is a default list of standard reagents
containing all reagents needed for the most
common staining methods.
• All reagents are listed in alphabetical order.
A blue background indicates that a reagent
has been selected.
• Before you allocate any reagents to any programs, make sure to update the reagent list
according to the reagents actually used, i.e.
add reagent names that are not part of the
list and delete reagents that are not used.
• The Reagent - Management - System (RMS)
can be switched on / off in this menu.
←→
Adding a reagent to the list
• Press NEW - the keyboard opens up.
↓
• E nter the name of the new reagent and confirm.
Up to 20 characters per reagent can be entered.
• The new reagent will be added to the table where it alphabetically belongs and highlighted with the blue bar.
↓
Leica ST5020
The alphabetical sorting function distinguishes between
small and capital letters as per the ASCII code. Capital
letters precede lower case letters.
47
5.Operation
If the Reagent Management System
(RMS) is switched on, the table headers are displayed as buttons.
Setting RMS data
The following limit parameters can be defined
for the RMS:
• Maximum number of days the reagent may
be used.
• Maximum number of racks that can be processed until the next reagent change.
↓
↓
Fig. 44
Deleting a reagent
↓
In 'instrument status' a red bar in the
station button indicates how long a
reagent has already been used depending on the values entered here.
The longer the red bar, the closer
the time when the reagent will have
to be changed. (See chapter 5.5.1)
Press the header buttons to access the corresponding numerical keypads for entering RMS
data.
Numbers between 1 and 99 can be entered in
both columns.
• Highlight the reagent you want to delete from the list.
• Press DELETE and confirm the screen prompt. The reagent is removed
from the list and the blue highlighting bar moves one row down.
Reagents can only be deleted if they are NOT allocated to any program or station. Otherwise there will be a message that deleting is
not possible.
48
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
The RMS log
→
→
→
→
The Leica ST5020 is equipped with a Reagent Management System (RMS) graphically representing processing history of reagents in 'Instrument Status' (see chapter 5.5.1).
These reagent data are stored in the RMS log table.
The RMS log table lists all reagent stations, the reagents allocated to each station as well as
their processing history.
Last change
Date when the reagent was last
changed.
Racks
Number of racks that have been
processed in a particular station since the reagent was last
changed.
Fig. 45
Information contained in a table row:
Station
Shows the numbers of those stations defined as
reagent stations.
Reagent
Reagent currently in a station or to be added to
that station. The Leica ST5020 does not recognize reagents automatically - reagents are allocated by entry into the reagent list.
Leica ST5020
Racks max.
Maximum number of racks that should be processed in a particular station without the reagent being changed.
Days
Number of days since the reagent was last
changed.
Days max.
Maximum number of days the reagent should
remain in the station without being changed.
49
5.Operation
5.4.8Station options
→
→
This screen provides an overview of all stations. Detailed information on individual stations can
be retrieved. Rack movements, however, are not shown.
8
Fig. 46
• Screen indication and functionality of stations are identical to the INSTRUMENT
STATUS screen (see Fig. 49).
• Pressing a station button
opens up the STATION DETAILS menu (Fig. 51).
• Programs ready to be started are displayed above the
station buttons (8).
(Column 'ready to start' in
Programs list = yes, Fig. 54).
• Pressing one of these program buttons gives you
direct access to the PROGRAMMING menu of the
button pressed (Fig. 56).
Additional functions:
↓
50
• Button DEL.STATION:
At the prompt 'Delete all reagent stations?', press CONFIRM to define
all reagent-stations as empty and to delete all entries in the STATION
DETAILS menu.
None of the programs will then be 'ready to start' any longer and none
of the buttons (8) will be displayed.
If you wish to exit without making any changes, i.e. without deleting
any stations, press CANCEL.
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
• PRINT button:
Press for a printout of
the current station configuration.
Instead of the graphical screen display, a list (Fig. 47) will be printed,
which, in addition to the information
provided by the graphical screen, also
shows the reagents allocated to each
station.
Fig. 47
5.4.9Calibration dialog
→
In the CALIBRATION DIALOG menu you can
enhance or restore touch screen response to
buttons being pressed. We recommend to use a
pen for the calibration procedure.
The pen must be placed exactly on the red dot
(1). The dot will move as shown in Fig. 29. To
exit the menu, press the dot once more in Position 4.
Functional test:
Calibrate the screen and verify the result approaching one of the screen buttons from all
four sides - if the screen has been successfully
calibrated, the button may not appear as pressed
(wider border) before you actually touch it.
1
3
→
2
Standard appearance
Button pressed
Fig. 48
Leica ST5020
51
5.Operation
5.4.10Data backup
→
→
This menu allows all instrument data to be written to a PCMCIA card. It is even possible to load
saved data from such a card into the multistainer memory. The data can only be backed up in
supervisor mode (9, Fig. 48). If a staining program is running, it is therefore impossible to write
data to a PCMCIA card or load data from the card into the memory.
To ensure compatibility of card and instrument, the card must be formatted IN THE INSTRUMENT before its initial use. Using a PCMCIA card that was formatted on a PC (e.g. with Windows XP) can lead to loss of data.
Fig. 48
Formatting a PCMCIA card:
• Change to the Supervisor mode.
• Call up the keyboard (e.g. as required for entering the password, see chapter 5.4.3)
• Enter the command "#format a:".
The card is formatted. At the end of the process, the information line indicates whether the
formatting was successful or not.
The following data is saved or loaded:
• All settings in the USER INTERFACE and
MOVE menus as well as the print settings
from the DISPLAY/PRINT menu.
• The current station settings.
• The reagent list.
• The program list with all program details.
• All saved clips (code and color allocation).
• The error log (Errorlog.txt).
Fig. 49
Only the error log can be read directly from the card. All other data is stored in code form and
cannot be edited with normal PC tools (e.g. text editing).
52
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
Important!
At least 1 MB of free memory is required on the PCMCIA card.
Otherwise, data backup cannot be completed and the writing of the data to the card will appear
to take a very long time.
In this case (if data backup lasts longer than 2 min.), remove the card from the slot, replace it
with an empty card and repeat data backup.
Backup the data as follows:
↓
↓
• Insert a PCMCIA card into the card slot beside the mains switch (see
Fig. 1, page 11).
• Press the DATA BACKUP button in the SETTINGS menu. A window
with the buttons RESTORE and BACKUP then appears.
• Press RESTORE to write the data from the card to the multistainer
memory. The multistainer then always restarts automatically, acting
just as it does when switched on (chapter 5.1, page 28).
• While the data is being written, the buttons are masked and the text
"Backup inprogress..." appears.
• Press BACKUP to save the current instrument data on the PCMCIA
card. After securing the data, switch off the instrument before removing the card.
Caution!
When you press a button in the data backup window, all data saved on the PCMCIA card or
elsewhere in the instrument is automatically overwritten.
The data is always saved or loaded into the instrument in its entirety.
It is not possible to transfer individual files on their own (e.g. just the reagent list but nothing
else).
• If you do not want to back up any data after calling up the menu, e.g. if
you called up the menu by mistake or there is no (empty) PCMCIA card
available, do NOT insert the card into the slot and press the BACKUP
button to leave the data backup screen.
No data will be written and the text "Backup inprogress..." does not appear.
However, if you press RESTORE, the instrument will restart as described above.
Leica ST5020
53
5.Operation
5.5 Instrument status
→
INSTRUMENT STATUS is the standard screen of the instrument, displayed automatically when
the instrument is switched on (unless a different language is selected - s. chapter 4.1).
• INSTRUMENT STATUS may
also be accessed from the
main menu, in which case
a BACK button will be displayed in the menu bar.
• The graphical display provides information on station
configuration, reagent status
and current location of racks
during a program run.
• Like in STATION DETAILS,
programs ready to be started (with the exception of
programs defined as 'white')
are displayed above the station buttons (1).
1
Fig. 50
• If one of the colored program buttons (1) is pressed, the PROGRAMMING menu of the corresponding program opens up.
(See chapter 5.6 for details).
• The SCHEDULE button leads to the 'Schedule' menu, where all racks
currently being processed are listed in a table.
(See chapter 5.7 for details).
• The PAUSE button is displayed only while staining programs are in
progress.
Press PAUSE to:
interrupt rack processing - a window containing the button CONTINUE
will open up and alarm type 3 will be triggered. Press CONTINUE to resume processing.
54
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.5.1Station buttons
Each of the 40 stations is configured as a button that can be pressed to directly access the STATION DETAILS menu. The station buttons are divided into three lines containing different types
of information, depending on each individual configuration.
3
2
4
5
5.5.2Station types
• The first line contains the station number (2) plus a letter (3)
indicating the station type.
These are: R: Reagent station
L: Load station
O: Oven station
E: Unload station
W:Water station Empty stations do not carry any letters.
• At the left border of the button a bar (4) may be displayed,
providing different types of information, depending on the
type of station.
• The second and third line are activated when a station is
used in a program run.
6
• The first line of a station turns white (5) when the transfer
arm is located above that station.
• In the second line the program code (6) is displayed in
7
the clip color that has been allocated.
• The third line contains a rhombus (#) followed by the sequential number (7) of the rack. This rhombus counts the
number of racks which pass the station everyday. When
Fig. 51
no. 99 is reached and everyday at 24:00, the rack counter
is reset to "0".
Load station
• There is a maximum of four load stations (L33-L36), L36 being nonchangeable. Load stations do not have a bar on the left border.
• After a new rack has been placed into a load station, the second line
of the corresponding button indicates which program (color and code)
has been recognized and will be carried out.
The rhombus (#) indicates that the rack has been detected by the software and will be processed as soon as possible.
Leica ST5020
55
5.Operation
Unload station
• There is a maximum of four unload stations (E25 - E28) - E25 being nonchangeable. Unload stations do not have a bar on the left border.
• Completed racks are placed into the unload stations by the transfer
arm, always starting with station E25. Line 2 shows which program has
been completed, line 3 shows the rack number. The colored program
indication blinks in two-second intervals.
RMS off
RMS on
Reagent station
• If the RMS (Reagent Management System) is ON, the bars at the left
border of each reagent station indicate reagent life.
The higher the bar, the more time is still left until the reagent needs to
be changed.
• If one of the set parameters (Days max., Racks max.) has been exceeded, the bar blinks in two-second intervals.
Assigning the reagent
• Select a reagent station (via the corresponding button), and press the
REAGENT LIST button in the STATION DETAILS menu (Fig. 51).
• Select the required reagent in the table (see Fig. 42) and press CONFIRM. The selected reagent is now assigned to the station and entered
in the RMS protocol.
Water station
• If a bar is being displayed on the left, the station is ready for use.
• The color of the bar changes as follows:
- blue bar: water entering into and exiting from the station
- white bar: water in the station is not running
• The water flow is controlled automatically.
Oven station (optional)
• If a bar is being displayed on the left, the station is ready for use.
• The color of the bar changes as follows:
- red bar: oven heating up to set temperature.
- white bar: oven not heating.
• The heater function is controlled automatically.
56
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.5.3Select station type
↓
10
9
8
↑
↓
Fig. 52
Fig. 53
Station details
• Press one of the station buttons to access
the STATION DETAILS menu.
• If a station is empty, only the CHANGE STATION TYPE button (8) is displayed.
• If CHANGE STATION TYPE is pressed, the
menu STATION TYPE CHOICE (Fig. 53) opens
up where you can see which station types
can be allocated to a particular station. Buttons corresponding to station types which
cannot be selected are disabled.
• Pressing one of the enabled buttons changes the station type without any further confirmation being required and leads you back
to STATION DETAILS.
• Depending on the station type chosen, additional station information is displayed.
Information displayed for all station types (9):
- station number,
- station type,
- rack number.
Rack number only if a rack is in the station. If
there is no rack in the station, the symbol (-)
is displayed.
Additional information can be found in
the row below and on the right side of the
screen (10).
• Additional buttons allow the user to select
further settings, depending on the type of
station.
Caution!
The Leica ST5020 is not capable of verifying the actual content of a station. Therefore the user
MUST make sure that the information entered into the STATION DETAILS menu is consistent
with the reagent the station has actually been filled with.
Leica ST5020
57
5.Operation
Indications and buttons corresponding to the different station types
Reagent station
Additional buttons
To open the reagent list (chapter 5.4.7) for allocating reagents to stations.
Press UPDATE REAGENT after a reagent has been changed, in order to
• set the date of "Last change" to the current date;
• set the date in the "Use by date" row according to the RMS;
• Set the rack counter to "00".
Additional indication Reagent name:
Shows the reagent allocated.
If the RMS is on, the following parameters are displayed:
Date last change:
Date when the reagent was last changed.
Use by date:
Date when next change is required.
Rack counter:
Number of racks processed so far.
Racks max.:Number of racks as per RMS that can still be processed before the reagent needs to be changed.
Even when the RMS is switched off, an internal log is kept in the
instrument. Therefore, all data displayed after switching the instrument on are always up-to-date.
Oven station
Additional button
Additional indication
Pressing TEMPERATURE opens up the numerical keypad for entering an
oven temperature. Temperature settings between 40 and 70 °C (corresponding to 104-178 °F) may be selected.
Heating off/on - indication controlled by staining program.
Temperature in degrees Centigrade/Fahrenheit.
Ovens 1 and 2 (stations 37/38) and ovens 3 and 4 (stations 39/40) are
linked. All settings made for one of the ovens will automatically be
applied to the second one, too
Water station
Additional buttons: Additional indication.
58
none
Water flow yes/no - indication controlled by staining program.
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.5.4Change station type
Load/unload station → Reagent station
• Load stations L33 - L35 and unload stations E26 - E28 can be defined as
reagent stations, if needed.
Fig. 54
5
↓
Tap water station → • Both station types must be redefined station by station (one station at
a time), starting from the 'center'; i.e., if you wish to convert load and/
or unload stations into reagent stations, you must start by converting
stations E28 or L33, respectively.
• The CHANGE STATION TYPE button (5) only becomes available to the
stations located further towards the outside after stations E28, or L33,
respectively, have been converted into reagent stations.
The same, in reverse order, applies for converting stations back into
load / unload stations.
• Press CHANGE STATION TYPE (5) to access the STATION TYPE
CHOICE menu.
Proceed further as described in chapter 5.5.3.
Reagent station
• It is also possible to convert the water stations W7 - W12 into reagent
stations, if needed. No particular order must be followed in that case.
• Follow the same procedure as described above. In addition, however,
the yellow tap water containers must exchanged for reagent containers to prevent reagents from spilling.
If the RMS is on, all reagent stations will be registered, including
those that have been converted into reagent stations later.
Leica ST5020
59
5.Operation
5.6 Staining programs
→
The PROGRAMS menu includes a table listing all staining programs stored.
In supervisor mode (Fig. 54) the column headers CLIP COLOR, ABBR. and PROGRAM NAME
are displayed as buttons. In addition, the buttons DETAILS, COPY, DELETE and NEW are
displayed, i.e. programs can be edited in this screen.
• Up to 50 different staining
programs can be entered
and stored.
• Existing programs may be
copied and/or edited.
• The DURATION column displays the total duration of all
program steps (in h:min:sec).
• The READY TO START column (yes/no) indicates
whether a program is ready
to be started.
Fig. 55
Individual button functions
• NEW creates a new (empty) program, located at the end of the program list.
• Programs selected to be copied are always copied to the last row of
the program list. All settings of copied programs are identical to those
of the original ones and can be edited. The clip color allocated is always WHITE.
• Switches to the PROGRAMMING menu (Fig. 57) where the individual
program steps are displayed. In supervisor mode new program steps
may be edited.
60
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.6.1Creating programs
↓
Creating new programs
• Press NEW (see Fig. 55) to create a new program row.
• Press PROGRAM NAME in the table header and enter a new program
name via the KEYBOARD.
• Press ABBR. to allocate a 3-digit code to the new program. This code
will be displayed in the INSTRUMENT STATUS and STAINING SCHEDULE menus.
• Press CLIP COLOR to access the PROGRAM
CLIP COLOR ASSIGNMENT menu.
• Press a color button to allocate a color to
the program selected.
The 3-digit program code will be displayed
on the color button.
Fig. 56
↓
↓
Leica ST5020
• Each color (except the color white) can only
be allocated to one program at a time.
Colors already allocated are identified by the
corresponding three-digit program code and
will be displayed as 'pressed' (wider border).
• Existing color allocations may be changed at any time. For that purpose, go to the program list (see Fig. 55 for further details) and highlight the program to which you want to allocate a different color. In
the example, the color red is to be allocated to H&E (previously it was
green). Then press CLIP COLOR to access the PROGRAM CLIP COLOR
ASSIGNMENT menu (Fig. 56). Press the red color button here.
• If the color you have selected was already allocated to a different program (color button contains 3-digit program code, PAP in the current
example), you must confirm that the color will be taken away from that
program.
• Press CONFIRM in the screen prompt: the selected color is allocated
to the selected (H&E) program. The program to which that color had
been allocated up to that point (PAP) will be defined as white (in the
CLIP COLOR column).
61
5.Operation
5.6.2Defining the program steps
• Press DETAILS (Fig. 54) to access the PROGRAMMING menu (Fig. 56).
If a program is created from scratch, the list of the individual program
steps is completely empty.
• The number in the STEP column indicates the order in which the stations will be used.
Press INSERT ROW - a new program row (step) is created in the row
below and highlighted in blue/red.
↓
1
2
Fig. 56
• The total number of steps (new
programs automatically start from
number "1") is automatically increased by 1.
The DURATION, EXACT and DIP
columns already contain standard
settings (2) which must be entered
depending on the program step.
• Code and name of the program (1)
are located above the table containing the program steps.
• Program steps are displayed in the
order in which they are supposed
to be carried out.
• Up to 40 steps can be defined for
each individual program.
For a complete definition of a program step, the following parameters
have to be entered:
1. Station in which the step is supposed to be carried out.
2. Reagent corresponding to that step (must be allocated to the station).
3. Duration of the step.
4. Step duration must be defined as exact / non-exact.
5. Dips (yes/no).
6. Oven temperature (optional)
62
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
Defining the program steps (continued)
Selecting a station for a step
• Click on STATION in the header row, the
CHOOSE STATION menu opens up (Fig. 58).
• To allocate a station to the step, press the
corresponding station button. The station
(button) is displayed as pressed, and the
lower row shows "P 1".
↓
Fig. 58
• Pressing CONFIRM assigns the selected station to the step, and the display
switches to the PROGRAMMING
menu (Fig. 60).
• Pressing RMS gives you access to the RMS
protocol (Fig. 59) – a table providing information on the current configuration (which reagent in which station).
See also chapter 5.4.7 RMS.
Fig. 59
Important!
Each station may only be selected
once in a program. Otherwise, the REAGENT column is highlighted in red,
and the program cannot be started.
• If a reagent has already been assigned to
the selected station (recommended), it will
be displayed in the REAGENT column.
In this case, the row for the step is highlighted in blue.
Fig. 60
Leica ST5020
63
5.Operation
Assigning reagents
• If no reagent is assigned to a program step,
press REAGENT in the header row to switch
to the reagent list (see chapter 5.4.7).
Highlight one of the reagents in the list and
press CONFIRM to assign it to the program
step.
↓
Fig. 61
↓ 1
• The reagent is now assigned to the step but
not yet to the station in which the step must
be executed.
For this reason, the row is highlighted in red
(1) indicating that the program step is not yet
ready to be started.
Assign the reagent selected for the step to
the correct station in INSTRUMENT STATUS
(see chapter 5.5.2).
This is required to ensure that the correct
reagent is in the station since the instrument
itself cannot detect it.
• Define the DURATION of the step, whether the step is EXACT or NON-EXACT and
whether or not there are DIPS.
Fig. 62
↓
Defining the duration of a step
• Press DURATION and enter the desired duration of the step. Press
CONFIRM: the selected duration is displayed in the DURATION column.
Caution!
If no step duration is selected, the default duration "00:00:00" will be
used, i.e. the corresponding step will NOT be carried out.
64
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
Defining exact steps
As there is potential for timing clashes to occur when multiple
racks are present in the instrument, steps which require precise
timing are designated as 'exact' in the program.
• Press EXACT to alter between yes (=exact) and no (not exact).
• Immersion times at exact steps are achieved to within ± 1 second. Immersion time at non-exact steps is at least as long as defined, most
of the time however, non-exact steps take longer than defined, as
racks due for non-exact steps receive attention only as the transfer
arm becomes available after an exact step.
• Define 'exact' steps only when really needed. The more exact steps,
the slower and less flexible the instrument (instrument throughput decreases).
↓
Setting the dips
If you interrupt the staining with PAUSE, take care not to do so
while an exact step is in progress. Otherwise, the program will be
stopped and the duration of the step will be extended by the duration of the pause. This can the cause errors during staining.
If PAUSE buttun has been pressd druring an exact step is in process, a warnig message will be appear in the dialog box
• Press DIP to switch from yes (= Dips activated) to no and vice versa.
• Duration and speed of one complete up and down movement (dip) are
selected in the MOVEMENT menu. The parameters selected there will
be applied to all programs. Increasing numbers of dips reduce instrument throughput.
For exact steps the following applies:
If the immersion time of an exact step is shorter than the time to do
the set number of dips, only the number of dips that fit into the immersion time will be done.
Leica ST5020
65
5.Operation
Completing the program
• Once the parameters EXACT and DIP have been selected, the definition of your step is complete.
• Press INSERT ROW to create a new row for the next program step and
once again define all step parameters as described above.
• Keep creating program steps until your program is complete.
• Program steps whose definition is not correct (duplicate station assignment, incorrect reagent,...) are highlighted in red in the REAGENT
column. Such steps must be corrected accordingly.
• A program will not be ready to start until all details have been defined.
The last program step
Three different types of "last steps" can be defined:
1. Last step = unload station.
Go to CHOOSE STATION and select EXIT as last station.
2. Last step = transfer station to Cover Slipper (optional).
Go to CHOOSE STATION and select CV as last station.
The REAGENT column from the reagent list now allows selecting between CV program 1 to CV program 4.
The transferred rack is automatically processed with the selected program (1 ... 4) in the CV5030.
3. Last step = any station other than EXIT or CV.
Programs do not necessarily have to finish in the unload station, as a
particular staining protocol might e.g. have to end with a specific reagent not contained in the last station.
Be sure to define the DURATION of the last step. Otherwise it will
not be carried out (default duration 00:00 will be assigned). Otherwise, the program cannot be started.
66
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.6.3Assigning a clip color
In this menu, you tell the instrument what color the clips attached to the individual racks are.
You need to be in supervisor mode to assign colors to clips.
Automatic start
Every time the load drawer is closed, the loading
area is scanned for transponder signals coming
from the clips attached to the racks in that area.
• In supervisor mode, the instrument automatically switches from INSTRUMENT STATUS
to CLIP COLOR if an unknown clip code is detected on a rack.
• If in user mode, a message is displayed telling the user that supervisor mode is required
and asking whether to switch to that mode
(see chapter 5.8.2).
If an unknown clip is detected, proceed as follows:
Fig. 63
→
1. Select the station carrying the unknown clip - the station is displayed
as having been pressed (wider border) - as e.g. station L34 in (Fig. 60).
2. Press the color button corresponding to the actual color of the clip
on the rack. The color selected will be displayed on the station button
(here: L34).
3. Press CONFIRM to go back to INSTRUMENT STATUS. The color will
be allocated to the station (clip) as selected. If the selected color was
already allocated to a different program, the 3-digit program code of
that program will be shown as well (see also Creating programs chapter 5.6.1). Otherwise, three question marks "???" will be displayed instead of a program code.
If the clip color 'white' is selected, the instrument will switch to
MANUAL PROGRAM START, where any program can be selected
and started (see chapter 5.8.2).
Leica ST5020
67
5.Operation
→
→
↓
Manual color allocation
Go to OPTIONS to assign a new
color to a clip already linked to
a different color.
Example:
A purple clip currently not
needed needs to be defined as
'white', as a white clip is needed but currently not available.
Proceed as follows:
• Place a rack bearing the clip
the color of which you want
to redefine into any one of
the load stations and close
the drawer.
Fig. 64
• The clip is now identified via the transponder signal.
Its current allocation (color - program) is displayed.
• Press CLIP COLOR.
• Clip color and program code are displayed on the station button.
• Select the station (L34 in our example).
• Press the color button containing the new color you wish to allocate to
the clip.
• The new color and, if that color was allocated to a different station before, the station code, is/are displayed on the station button.
• Press CONFIRM to complete the color allocation procedure.
• As the color 'white' has been selected, the instrument switches automatically to MANUAL PROGRAM START, where you can allocate the
desired program to the rack which is now equipped with a 'white' clip.
• Once the program has been completed, you can repeat the above procedure to return the clip to its original (true) color.
68
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.7 Programs in process
→
or
→
PROGRAMS IN PROCESS is a table listing all programs currently in progress. It can be displayed instead of instrument status, as PROGRAMS IN PROCESS also provides information on
all important run times.
The table contains the following
information:
Rack
Rack number - as in instrument
status.
Staining protocol
3-Digit code and clip color allocated to a program.
Station
Indicates current rack position.
GA means that a rack is currently being transferred.
Exact (yes/no)
Provides information on whether a step has been defined as
"exact" or "non-exact".
Fig. 65
Press SORT to switch back and forth between
two sorting options: by rack number and by total remaining processing time - the button will
change accordingly.
List sorted by:
↔
Increasing rack
number
Leica ST5020
Total remaining
processing time
Remaining station time
Shows the remaining immersion time of the
rack in the station.
Total remaining processing time
Total time remaining for the rack until the current program is completed.
All times are based on defined program step
times and do not include any delays caused by
non-exact steps. Times displayed are updated
each sec. as well as each time a new racks
starts being processed.
69
5.Operation
5.8Staining
Prior to starting a staining program, thefollowing preparatory steps must be taken:
• Optimize all USER INTERFACE parameters.
• Programs needed for staining must be
'ready-to-start'.
• Stations have to be configured and filled according to configuration.
• Racks needed for staining must be equipped
with a clip and filled with the slides to be
processed.
Place the clips (1) onto the rack with the
transponder (2) facing outwards.
• Make sure the instrument "knows" all clips
needed for staining.
Caution with sequences which involve
an oven at the initial step.
In this case the loading unit, out of
which the specimen holder is removed
via the transport arm, must NOT be filled
with an inflammable reagent (e.g. xylene).
As the oven temperature is up to 80 °C
(176 °F), the reagent may ignite and
cause damage to the device and to the
samples.
For the same reason, steps conducted
INSIDE an oven must never be carried
out from a reagent station with inflammable reagents.
5.8.1Loading the racks
1
2
• Racks may only be loaded via the load stations (front right of the instrument).
• Open the load drawer and insert the racks
as shown in Fig. 66 in one of the load stations with the clips facing outwards (towards the user).
• Each rack must bear a clip corresponding to
the program to be run.
• Close the drawer completely.
For the clips to be recognized correctly,
the load drawer must be opened for loading and subsequently be closed again.
Do not insert the racks just opening the
lid!
Fig. 66
70
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
5.8.2Starting programs
→
• Once the drawer is closed, the load stations are scanned in order to
identify the clips on the stations that have been loaded.
4
• Once identified, the clip colors / program codes are displayed in INSTRUMENT STATUS (3) (see also chapter 5.5).
Automatic program start
• Once all components have been identified and are ready to be started,
the staining programs will start automatically.
For programs to be started (automatically), the instrument must be at
user access level (4).
If there are already some staining programs running, the new racks
are integrated into the overall process as soon as the software detects capacity for a new program start.
Racks are processed in the order of optimized throughput, not necessarily in the order in which they have been placed into the load
station.
3
Fig. 67
Loading a clip not allocated to a program (any longer)
• If a rack placed into the load station carries a clip which is not allocated to any program or the color of which is allocated to a program not
ready to be started, in the second line of the corresponding load station three question marks "???" will be displayed instead of a program
code.
• Processing options with such a clip depend on the current instrument
mode.
- In supervisor mode, the color of a 'ready-to-start' program can be
assigned to the clip (see chapter 5.6.3).
- In user mode, an error message will be displayed, specifying why
that particular rack cannot be processed. At the same time, the
corresponding alarm sound is triggered.
Remove the rack to acknowledge the error message and stop the
alarm; close the drawer.
Leica ST5020
71
5.Operation
Loading an unknown clip
• If a rack carrying an unknown clip is placed into the load drawer, the
CLIP COLOR menu opens up automatically.
If any staining processes are already in progress, a screen prompt will
open up inquiring whether the user wishes to access supervisor mode.
-CONTINUE stops the selection procedure and continues the staining processes in progress. The load station button will display three
question marks instead of a program code "???" and no color allocation will be visible. - The rack must be removed.
-ADMINISTRATOR opens up the keyboard for entering the password
and then switches over to CLIP COLOR for a color to be allocated.
(See chapter 5.6.3)
Caution - bear in mind any steps defined as exact!
During the color allocation procedure the instrument is paused.
The programs in progress are paused until the color allocation has been completed
and the instrument is switched back to user mode.
Manual program start
Manual start is recommended for programs less frequently used or if no
suitable colored clip is available for a particular program. If a rack carrying a (defined) white clip is loaded, MANUAL PROGRAM START opens up.
• A 'ready-to-start' program must be allocated
to the rack.
• Press START to go back to INSTRUMENT
STATUS. The rack will now be handled as if
carrying a colored clip and the staining process will start as normal.
The manual program allocation of the
white clip will be deleted when the program ends (and the rack is removed).
The clip can then be reallocate to any
program.
Fig. 68
72
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
A staining process can be interrupted:
• to gain access to the instrument in case any
problems occurring during staining require
user intervention.
• to check or change reagents.
• Press PAUSE in INSTRUMENT STATUS to
interrupt all staining programs in progress at
any given time.
5.8.3Interrupting a staining program
The PAUSE button is displayed only
when the instrument is in user mode
and at least one staining program is
being run.
Fig. 69
➤
Caution!
Before moving to the 'parking' position, the transfer arm completes
the movement cycle in progress. Wait UNTIL the transfer arm is in
the parking position before opening the instrument lid.
• While the instrument is in pause mode, alarm type 3 sounds and a dialog box (see left) indicates PAUSE mode.
• Press CONTINUE to resume staining.
Caution - exact steps!
If staining is interrupted, immersion times during PAUSE will not
be identical to those in the chosen program(s).
When PAUSE is pressed while an exact step is in progress, staining results might be negatively affected! The duration of the step
will be extended by the duration of the pause. If PAUSE buttun has
been pressd druring an exact step is in process, a warnig message
will be appear in the dialog box.
Programs cannot be modified neither while staining is in progress
nor in pause mode.
Leica ST5020
73
5.Operation
5.8.4Finishing programs
Unloading racks from the unload drawer
If a rack is located in the unload drawer, the alarm type 1 sounds.
• The code of the completed program and (in the bottom row) the basket
number are displayed in the unload station symbol. The colored program indication blinks in two-second intervals.
↓
• Carefully open the unload drawer and remove the rack. You may also
remove the entire reagent container together with the rack and replace it by another container.
• Close the unload drawer - program code and rack
number must disappear from the station button.
• If, despite the unload drawer having been opened
and closed several times, the instrument still displays code and rack number of the rack already removed, the transponder or the automatic recognition
system might be defective.
Press CANCEL RACK to manually 'unload' the clip.
Fig. 70
Removing racks from stations other than unload
If a program finishes in a station other than unload, alarm type 3 sounds
at the end of the program and there will be a screen prompt requiring the
user to remove the rack.
• Press CONTINUE to confirm that the rack has been removed from the
station.
This is necessary, as the instrument will otherwise not be able to recognize whether there is still a rack in that station or not.
• Confirmation of rack removal also switches off the alarm. The staining program is considered to be finished and removed from the list of
pending staining runs.
74
Instructions for Use, V2.0, RevG - 07/2016
5. Operation
Racks can be cancelled if needed.
Cancelling racks
↓
• Press STAINING SCHEDULE to access the staining schedule menu.
• Select the rack you wish to cancel (highlight
with blue bar).
• Press CANCEL RACK.
• Press YES to confirm.
If NO is pressed, the instrument will switch
back to the staining schedule menu without any
changes being made.
Fig. 71
↓
↓
• When pressing YES a second prompt opens up, where you need to
confirm that you have removed the rack from the station it was located
in last.
This step is necessary as the instrument itself is not able to detect
whether there is a rack in that station or not.
Wait until the transfer arm has finished the movement in progress
and is located in the parking position (rear left). Open the lid and
remove the rack. WAIT until the station number is displayed on the
screen - once this message is being displayed, confirm that the
rack has been removed.
• Press CONFIRM - the current staining run is aborted and removed from
the list of pending runs. Staining will be resumed starting with the next
rack.
If the program highlighted by the blue bar has already been completed (rack already located in unload station or just being transferred to
unload station), the CANCEL RACK button is disabled.
Leica ST5020
75
6. Cleaning and maintenance
6.1 Cleaning the instrument
Prior to cleaning the instrument, always switch off mains and unplug the power cord!
When handling cleaning detergents, follow the instructions of the
manufacturer and make sure all laboratory regulations in force in
your country are complied with.
Dispose of used reagents according to the laboratory regulations
in force in your country!
Immediately wipe away spilled solvents (reagents)! Solvent resistancy of the lid surfaces is limited if exposed to solvents for a
lengthy period of time!
Do not use any of the following for cleaning the outside surfaces
of the instrument: alcohol, detergents containing alcohol (window
cleaner!), abrasive cleaning powders, solvents containing acetone
or xylene!
No liquid may come into contact with the electrical connections
or spill into the interior of the instrument and/or the transport arm!
Interior
Transfer arm
Outside surfaces
Remove the reagent and wash vessels. Clean the stainless-steel inner
walls with a regular cleaner and rinse with water.
The transfer arm contains sensitive electronic components. Therefore do
not clean with any liquid but simply wipe down with a slightly moistened
cloth.
Clean the painted outside surfaces with a mild detergent and subsequently wipe down with a moistened cloth.
Do not use any solvents for cleaning the outside surfaces and/or the lid!
TFT touch screen
Use a commercial screen cleaner for cleaning the touch screen. Use as
directed by the manufacturer.
76
Instructions for Use, V2.0, RevG - 07/2016
6. Cleaning and maintenance
Wash and reagent vessels
Clean the wash and reagent vessels in the laboratory dishwasher at maximum +65 °C. Use a standard laboratory dishwasher detergent.
The vessels may not be exposed to temperatures any higher than
65 °C (e.g. do not clean in industrial dishwashers, which run at a
temperature of 85 °C), as the stations might become deformed!
Drain outlet / hose
Slide racks
Oven
From time to time check the waste water drain hose for accumulated dirt,
particularly for algae. Clean whenever necessary.
To prevent the growth of bacteria, the drain system may be flushed with
5 % sodium hypochlorite. Metal parts may, however, not be exposed to
this solution for a lengthy period of time (e.g. overnight). Therefore, rinse
with abundant water after cleaning with sodium hypochlorite.
Clean with household or laboratory cleaners, as needed.
Regularly check the paraffin collecting tray(s) at the bottom of the oven(s)
for paraffin residues and clean, if necessary.
6.2 General maintenance
The instrument is virtually maintenance-free in operation.
To ensure smooth operation of the instrument over many years we do,
however, recommend the following:
• Have the instrument inspected once per year by a qualified service engineer authorized by Leica.
• At the end of the warranty period, enter into a service contract. For
more information, please contact your local Leica technical service
center.
Leica ST5020
77
6. Cleaning and maintenance
6.3 Preventative maintenance schedule
Maintenance activity
daily
Clean the TFT screen with a lint-free cloth.
Follow the manufacturer's instructions when using
screen cleaner.
√
Clean outside surfaces of instrument and lid with soft
cloth.
Use cleaning detergent, if necessary.
√
weekly
Clean wash vessels.
Check the wash vessel O-rings for damage and lubricate
them slightly.
See Fig. 4, page 16 for further details.
√
Remove and clean the reagent vessels. A dishwasher up
to 65 °C can be used.
√
Check waste water hose for accumulated dirt and clean,
if necessary.
√
Remove paraffin residues from paraffin collecting tray(s)
of the oven(s).
√
monthly
Check strainer (V-Filter, Pos. 3 in Fig. 6, page 18) in tap
water inlet hose for accumulated dir tand clean, if necessary.
√
Check air vent holes of fans for dust. Clean, if necessary.
√
Check the tap water inlet hose connection for damage.
If necessary, replace the gasket as described in chapter 4.3, page 18.
√
Replace activated carbon filter as described in chapter 4.6, page 24.
√
Check electrical connections at rear panel of instrument.
√
78
Instructions for Use, V2.0, RevG - 07/2016
7. Troubleshooting
7.1General
The Leica ST5020 is equipped with a troubleshooting system.
If an error is detected, detailed error messages and corresponding user
instructions on what to do are displayed on the touch screen.
For all correctable error conditions the instrument will take corrective actions to protect the specimens.
Details of all staining protocols carried out are recorded in the daily run
log. The daily run log can be viewed and/or printed in the VIEW/PRINT
menu.
7.2 Correcting errors
If any programs are in progress, check the job queue containing the staining programs to be run.
If all programs are completed, check the error log for error messages:
access the error log (either view in menu VIEW/PRINT --> ERROR LOG) or
print it out - entries are listed in chronological order.
7.2.1Excessively high water level in the drain pan
Whenever the water level in the drain pan reaches the level sensor
(Fig. 72, 1), an alarm message is triggered and the water inflow into the
water flow cuvettes (7-12) is stopped for safety reasons. The water inflow
is automatically turned on again whenever the water level is below the
level sensor.
Sources of errors:
1. The water drain or the drain tube is blocked or clogged. The water
level in the drain pan has reached the level sensor; the water does not
drain.
2. The water drain or the drain tube is partially clogged or blocked. The
water level in the drain pan has reached the level sensor; the water
drains only slowly.
3. The level sensor is dirty.
Leica ST5020
79
7.Troubleshooting
1. Read the error message, acknowledge the alarm and, immediately
afterward, press the "PAUSE" knob. The transport arm moves into a secure position. The instrument is now in pause mode and the instrument
cover can be opened.
Caution!
As long as the level sensor is covered with water, the water flow
cuvettes' (7-12) water valves are closed. The cleaning process in
the water flow cuvettes is interrupted. No fresh water flows into
the water flow cuvettes.
Fig. 72
Subsequently, remove cuvettes 1 and 13 (see label in Fig. 72). Inspect the
water drain for clogs and the level sensor for dirt.
Caution!
If there are specimens in cuvettes 1 and 13, these must be secured
by the user and manually removed from the relevant cuvettes and
out of the instrument as necessary. Depending on the relevant
staining steps at the time of the alarm, the user must secure the
specimen so that no damage to the specimen results (for example,
by transferring them into suitable, compatible reagents).
2
1
Fig. 73
80
During a period of approximately 10 seconds, monitor to see whether
the water level in the drain pan drops slowly or remains high.
Remove visible blockages to the water drain (Fig. 73, 2) and dirt on the
level sensor (Fig. 73, 1).
The staining process can go forward if sufficient water drainage is
again ensured.
For this purpose, reinsert cuvettes 1 and 13 in the instrument. The user
must decide at this point whether the specimens taken from cuvettes
1 and 13 can be reused or whether these should be stained manually
until finished. In this case, the respective programs must be canceled!
(refer to chapter 5.8.4 - Quitting programs).
Restart the staining process by pressing the "CONTINUE" button (refer
also to chapter 5.8.3 - Interrupting staining).
Instructions for Use, V2.0, RevG - 07/2016
7. Troubleshooting
After ending all staining programs, switch off the instrument and
clean the water drain and the drain tube. It is recommended to insert a cleaning tablet with active oxygen into the water drain, let it
work in, and flush with a little water.
Caution!
If there are specimens in cuvettes 1 and 13, these must be secured
by the user and manually removed from the relevant cuvettes and
out of the instrument as necessary. Depending on the relevant
staining steps at the time of the alarm, the user must secure the
specimen so that no damage to the specimen results (for example,
by transferring them into suitable, compatible reagents).
Programs already started and assigned to the manually removed specimen slide holders must be ended with the "CANCEL RACK" function
(refer to chapter 5.8.4 - Quitting programs).
Important information!
When ending the programs with the "CANCEL RACK" function, a
later proper resumption of the staining process for other specimen
slide holders is ensured.
If it is not possible to ensure a lasting and sufficient drainage of
water from the instrument by cleaning the water drain and the
drain tube, the instrument must be switched off and the responsible Leica service department must be contacted.
Leica ST5020
81
7.Troubleshooting
7.3 Power failure
• Check whether there is a general power failure (no mains power).
• Check whether the mains plug is inserted correctly into the wall outlet
and whether the wall outlet is switched on, if applicable.
• Check whether jumper cable (or UPS cable) is inserted correctly into
the corresponding sockets.
• Check whether the mains switch is switched on correctly.
• Check whether one of the four secondary fuses is defective.
Some instrument malfunctions / failures are caused by defective fuses.
Power failure
Malfunction / failure
Fuse to be checked
• Instrument not working - no response.
• No data can be entered via touch screen
or touch screen darkened.
• Stepper motors do not work.
•
•
•
Instrument does not work properly.
Instrument cannot be operated at all
or instrument responses are faulty.
Erroneous display indication.
•
•
•
•
Wash stations emptying (solenoid valves
not being addressed).
Extraction fan not working.
Transfer arm not moving.
Oven malfunction (oven fan).
• No vertical transfer arm movement.
• Transfer arm brake system not working.
82
Fuse F1
Fuse F2
Fuse F3
Fuse F4
Instructions for Use, V2.0, RevG - 07/2016
7. Troubleshooting
7.4 Replacing the secondary fuses
Prior to exchanging a fuse, always switch the instrument off and unplug the mains cord.
Defective fuses may be replaced only with the replacement fuses supplied together with the
instrument.
Important!
Follow the instructions below to make sure you select the correct replacement fuse for each
fuse holder.
Selecting the correct replacement fuse
The amp value (here: 2A) printed on the fuse and the amp value next to
the fuse holder on the rear panel of the instrument MUST be identical!
Four fuse holders, marked
F1 through F4, are located at
the rear panel of the instrument, below the power supply sockets.
Check the malfunction table
on page 78 to find out, which
fuse needs to be replaced.
Example:
Touch screen failure:
--> Exchange fuse F1.
Fig. 74
The fuse value is shown on the metal caps
of the replacement fuses - the imprint looking more or less as indicated above (may vary
slightly depending on the fuse type).
Amperage (A) is the parameter that is important
for allocating fuses correctly to the corresponding fuse holder (in the example above: F1/T2A)
Leica ST5020
To replace the fuse in fuse holder F1, select the
replacement fuse marked '2A' (see Fig. 69).
Only the fuse marked '2A' can be inserted into
fuse holder F1. The same applies for the remaining fuse holders F2 - F4, i.e. always make
sure the amp rating on the fuse corresponds to
the amp rating indicated above the fuse holder.
83
7.Troubleshooting
Replacing the fuses
1
3
2
2
Fig. 75
Fig. 76
• Insert a screwdriver (1) into the slot on fuse
holder (2) and push slightly inwards rotating the screwdriver 1/4 turn to the left at the
same time.
• Remove the defective fuse (3) from the fuse
holder (2) and insert the correct type replacement fuse.
• The fuse holder is released and can be removed.
• Reinsert the fuse holder together with the
fuse into the instrument housing. To lock the
fuse holder in place, insert the screwdriver
into the slot, push slightly inwards and rotate
1/4 turn to the right at the same time.
84
Instructions for Use, V2.0, RevG - 07/2016
8. Warranty and service
Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control
procedure based on the Leica in-house testing standards, and that the
product is faultless and complies with all technical specifications and/or
agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded
agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall
apply exclusively.
Technical service information
If you require technical service or replacement parts, please contact your
Leica sales representative or dealer who sold the product.
Please provide the following information:
•
•
•
•
Model name and serial number of the instrument.
Location of the instrument and name of the person to contact.
Reason for the service call.
Date of delivery.
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of in compliance with the local laws.
To prevent damage to the instrument or the specimen, only accessories authorized by Leica may
be used.
Leica ST5020
85
86
Exit
Exit
Reagent
Reagent
Station 26
Station 14
Station 13
Station 25
Reagent
Reagent
Reagent
Exit
Station 27
Reagent
Station 15
Station 3
Station 2
Station 1
Staining:
9.1 Container Map
Exit
Station 28
Reagent
Station 16
Reagent
Station 4
Station 30
Reagent
Reagent
Reagent
Station 18
Reagent
Station 6
Station 29
Reagent
Station 17
Reagent
Station 5
Reagent
Station 31
Reagent
Station 19
Reagent
Station 7
Reagent
Station 32
Reagent
Station 20
Water
Station 8
Load
Station 33
Reagent
Station 21
Water
Load
Station 34
Reagent
Station 22
Water
Station 10
Oven
Oven
Station 9
Station 38
Station 37
Load
Station 35
Reagent
Station 23
Water
Station 11
Oven
Station 39
Load
Station 36
Reagent
Station 24
Water
Station 12
Oven
Station 40
9.Appendix
Instructions for Use, V2.0, RevG - 07/2016
Leica ST5020
Reagent
Reagent
Station 14
Reagent
Station 26
Exit
Reagent
Station 13
Reagent
Station 25
Exit
Exit
Station 27
Reagent
Station 15
Station 3
Station 2
Station 1
Staining:
Container Map
Exit
Station 28
Reagent
Station 16
Reagent
Station 4
Station 30
Reagent
Reagent
Reagent
Station 18
Reagent
Station 6
Station 29
Reagent
Station 17
Reagent
Station 5
Reagent
Station 31
Reagent
Station 19
Reagent
Station 7
Reagent
Station 32
Reagent
Station 20
Water
Station 8
Load
Station 33
Reagent
Station 21
Water
Load
Station 34
Reagent
Station 22
Water
Station 10
Oven
Oven
Station 9
Station 38
Station 37
Load
Station 35
Reagent
Station 23
Water
Station 11
Oven
Station 39
Load
Station 36
Reagent
Station 24
Water
Station 12
Oven
Station 40
9. Appendix
87
9.Appendix
9.2 Optional accessories
To prevent damage to the instrument or the specimen, only accessories authorized by Leica may
be used.
Reagent vessels, assy. (with handles and lids)
Wash vessels, assy. with connection piece
Lid, slotted - for reagent vessel
Lid, w/o slots - for reagent vessel
Slide rack 30 - pack of 1 - plastic
Slide rack 30 - pack of 5 - plastic
Slide rack 30, metal
Rack - special stainings
Insert - special staining
Lid for 12 vessels, assy.
Adapter - Sakura Adapter Medite 20
Adapter Medite 30
Adapter Shandon
Adapter Microm 30
Clips, assy. - white Pack of 5 Clips, assy. - yellow Pack of 5 Clips, assy. - light blue Pack of 5 Clips, assy. - dark blue Pack of 5 Clips, assy. - pink Pack of 5 Clips, assy. - red Pack of 5
Clips, assy. - light green Pack of 5 Clips, assy. - purple Pack of 5 Clips, assy. - grey Pack of 5 Tap water inlet hose, 2.50 m length, assy. with 3/4" connection for water tap
'V' strainer 3/4"-40/22 A6 (strainer for inlet hose)
Set of gaskets for tap water inlet hose
88
Order No.
14 0475 33659
14 0475 33660
14 0475 34486
14 0475 34488
14 0475 33750
14 0475 33643
14 0456 33919
14 0475 34524
14 0475 34525
14 0475 33644
14 0475 34515
14 0475 34516
14 0475 34517
14 0475 34518
14 0475 34943
14 0475 33632
14 0475 33633
14 0475 33634
14 0475 33635
14 0475 33636
14 0475 33637
14 0475 33638
14 0475 33640
14 0475 33641
14 0475 32325
14 0456 36101
14 0300 00132
Instructions for Use, V2.0, RevG - 07/2016
9. Appendix
Optional accessories
Jumper cable - mains
Vessel holder, assy.
Hose clamp for waste water hose
Waste water hose 2 m
Waste water hose 4 m
Exhaust air hose 2 m assy.
Exhaust air hose 4 m assy.
Remote alarm jack - 6.3 mm Oven - Leica ST5020, assy.
Leica TS5025 - Transfer station, assy. ST5020 --> CV5030
Demo - Box Leica ST5020
Activated carbon filter
Base plate ST5020/CV5030
Leica ST Ultra - Clearing Agent
Special O-ring grease - 25 ml Haas OHA
PCMCIA Flash Card, with adapter
Compact flash card adapt.
Compact flash card 16MB
Heatable reagent st. kit
Wash vessel module - 1 wash vessel 1 tap water inlet hose 2.5 m replacement fuses:
fuse T 2.0 A
fuse T 2.5 A
fuse T 4.0 A
Leica ST5020
Order No.
14 0411 34604
14 0475 34548
14 0422 31972
14 0475 35747
14 0475 35748
14 0475 35762
14 0475 35763
14 6844 01005
14 0475 34611
14 0478 36703
14 0475 36230
14 0474 32273
14 0475 37647
14 0709 36260
14 0336 36657
14 0475 42526
14 0475 37684
14 0475 37683
14 0475 34612
14 04753 7071
14 6943 02001
14 6943 02501
14 6943 04001
89
Notes
90
Instructions for Use, V2.0, RevG - 07/2016
www.LeicaBiosystems.com
Leica Biosystems Nussloch GmbH
Heidelberger Straße 17-19
D- 69226 Nussloch
Phone:+49 6224 - 143 0
Fax: +49 6224 - 143 268
Internet: www.LeicaBiosystems.com
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