Leica Application Suite LAS V3.3

Leica Application Suite LAS V3.3
Leica Application Suite
LAS V3.3
LAS Installation Guide
LAS Installation Guide
All reasonable steps have been taken to ensure that this publication is correct
and complete, but should any user be in doubt about any detail, clarification may
be sought from Leica Microsystems (Switzerland) Ltd., or their accredited
representative. The information in this document is subject to change without
notice and should not be construed as a commitment by Leica Microsystems
(Switzerland) Ltd.. Leica Microsystems (Switzerland) Ltd.accepts no
responsibility for any errors that may appear in this document.
© 2008 Leica Microsystems (Switzerland) Ltd
No part of this publication may be reproduced, transmitted, transcribed, stored
in any retrieval system or translated into any human or computer language by
any means or in any form, without the prior written permission of Leica
Microsystems (Switzerland) Ltd.
Due to a policy of continuous development, we reserve the right to change
specifications without notice.
Microsoft and MS-DOS are registered trademarks and Windows, the Windows
logo, the Windows Vista logo, the Windows 2000 logo and the Windows XP
logo are trademarks of Microsoft Corporation.
Date: November 2008
Software Version: LAS V3.3
Leica Microsystems (Switzerland) Ltd.
Stereo and Macroscope Systems
CH 9435 Heerbrugg
Switzerland
Telephone: +44 1223 411411
FAX +44 1223 412526
Hotline: +44 1223 401824
di.support@leica-microsystems.com
www.microscopy-imaging.com
LAS Installation Guide
At Leica Microsystems Cambridge Ltd. we are continually striving to improve the
standards of our manuals and would welcome customer feedback. If you would
like to comment on any aspect of this manual or our manuals in general, please
send email to:
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Please quote the title and date of the manual. These can be found on the
previous page.
Table of Contents
LAS Installation Guide
Starting Installation
......................... 1-3
................................................................... 1-4
Preparatiion
................................................................... 1-5
Installing with Windows
XP
...................................................................
1-6
Installing with Windows
XP (continued)
...........................................................................................
1-7
Sentinel Protection Installer
Warning
...........................................................................................
1-8
Minimum and Custom...........................................................................................
Setup
1-9
Microsoft Security Update
........................................................................................... 1-10
Connecting Camera ...........................................................................................
and Microscope
1-11
Installing with Windows
Vista
...................................................................
1-12
Enhanced Security Features
........................................................................................... 1-13
Minimum and Custom
Setup
...........................................................................................
1-14
Vista Aero
........................................................................................... 1-15
Connecting Camera ...........................................................................................
and Microscope
1-16
Selecting Hardware ................................................................... 1-17
Selecting Hardware ...........................................................................................
(continued)
1-18
Selecting Microscope
........................................................................................... 1-19
Selecting Microscope
..........................................................................................
(continued)
1-20
Selecting Camera ........................................................................................... 1-21
Optional Modules (Evaluation)
................................................................... 1-22
Evaluation (continued)
........................................................................................... 1-23
Demo Licence
........................................................................................... 1-24
Licensing
........................................................................................... 1-25
Licensing (continued)
.......................................................................................... 1-26
Licensing (continued)
.......................................................................................... 1-27
Installing Power Mosaic
Hardware
...................................................................
1-28
Oasis-blue Board
........................................................................................... 1-29
Oasis-blue Board (continued)
.......................................................................................... 1-30
Power Mosaic with Windows
XP
...........................................................................................
1-31
Oasis-blue with Other
Hardware
...........................................................................................
1-32
Oasis-blue with Other
..........................................................................................
Hardware (continued)
1-33
Oasis-blue with Other
..........................................................................................
Hardware (continued )
1-34
Oasis-blue with Other
..........................................................................................
Hardware (continued)
1-35
Check Oasis-blue Settings
........................................................................................... 1-36
Check Oasis-blue Settings
..........................................................................................
(continued)
1-37
Installing Stage with...........................................................................................
Encoders
1-38
Installing Stage with
..........................................................................................
Encoders (continued)
1-39
Installing Stage with
..........................................................................................
Encoders (continued)
1-40
Installing Stage with
..........................................................................................
Encoders (continued)
1-41
Further Support
........................................................................................... 1-42
Chapter 1
LAS Installation Guide
LAS Installation Guide: Introduction:
This document describes how to install
the Leica Application Suite: Please read
it before installing your copy of this
software.
It also acts as a supplement to the
User Guide that is on the LAS
installation disk.
All reasonable steps have been taken
to ensure that this publication is correct
and complete, but in case of doubt
please seek clarification from Leica
Microsystems (Switzerland) Limited, or
their accredited representative.
The information in this document is
subject to change without notice and
should not be construed as a
commitment by Leica Microsystems
(Switzerland) Limited.
Leica Microsystems (Switzerland)
Limited accepts no responsibility for any
errors that may appear in this
document.
Copyright © 2008 Leica Microsystems
(Switzerland) Limited.
All rights reserved. The contents of
this publication may not be reproduced
in any form, or communicated to a third
party without prior written permission of
Leica Microsystems (Switzerland)
Limited.
Due to a policy of continuous
development, we reserve the right to
change specifications without notice.
Microsoft and MS-DOS are registered
trademarks and Windows, the Windows
logo, the Windows Vista logo the
Windows XP logo are trademarks of
Microsoft Corporation.
November 2008. Issue: 2. Printed in
UK.
1-3
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide : Starting Installation:
Leica Application Suite Version is
supplied on DVD.
Please read the System
Requirements and Release Notes
before starting the installation. Adobe
Acrobat Reader must be installed on the
computer to display the files. If it is not,
the program is available on the DVD
from the Documents and Drivers
section..
1: Insert the DVD drive. The start up
screen should appear
automatically.
2: Click on the Drivers, Examples and
Documents button and then System
Requirements for Using LAS button
which will start Adobe Acrobat
Reader and display the file.
3: Click on the Release Notes button
and again, Acrobat will start and
display the file.
4: If the DVD does not start up
automatically, double-click the DVD
drive in Windows Explorer.
5: From the list of files on the DVD,
double click either System
Requirements.pdf or Release
Notes.pdf to display the files.
If LAS Power Mosaic is to be used,
please see the installation information in
this document. LAS Power Mosaic
cannot run until the appropriate
installation is complete. This must
include the installation and configuring
of the Oasis drivers.
LAS Installation Guide
1-4
LAS Installation Guide: Preparation:
The LAS software to control a
microscope and Leica DFC camera, can
be used in accordance with the software
license. Optional modules may be used
for a 60-day evaluation period but after
that they must be licensed for continued
use.
If Leica AF6000 software is also to be
used then it must be installed before
LAS. If Leica AF6000 is subsequently
re-installed, LAS must also be
re-installed afterward.
Preparation:
Ensure the computer conforms to the
System Requirements. If Windows Vista
is the operating system, make sure the
Windows Experience Index is 3.5 or
higher.
System Restore: (1)
Create a System Restore Point using
the wizard. For guidance read the
instructions in Windows Help.
Languages:
Windows installs different languages for
its user interface by means of the
Multi-lingual pack. Check that the
version of Windows you are using
supports the multi-lingual pack for the
language you require for Japanese and
Chinese. Make sure you have installed
the language you require before you
install LAS. The language selected for
Windows operation determines the user
interface language used by LAS. If LAS
supports the Windows selected
language, LAS will use this. If LAS does
not support this language, the user
interface will be displayed in English.
1-5
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Installing with Windows XP:
Leica DFC Twain camera software is installed for the
language being used by Windows at the time of LAS
installation. If the language is subsequently changed
LAS will change accordingly, but the camera software
will not. It must be re-installed with the appropriate
language selected.
Installation for Windows XP and Vista are treated
separately as follows:
Installation Steps: Windows XP only:
· Install the microscope following the instructions
provided.
Please note that if you are to install Leica AF6000
software on the same PC, then it must be installed
before LAS. If you later reinstall Leica AF6000, it
will be necessary to reinstall LAS afterwards.
· Do NOT connect the interface cable between
the microscope and the computer.
· Do NOT connect the FireWire cable to the Leica
DFC camera until the software has been
installed.
The installation procedures assumes that all the
software components are installed on the first hard
disk drive (i.e. C: ) and for this release of LAS the
main application is installed there also. The
destination directory cannot be changed.
· Disconnect the computer from a network during
installation to prevent accidental loading of
drivers from the Internet if an incorrect option is
chosen. All LAS microscope and camera drivers
are on the supplied LAS DVD. Do not load any
from the Internet.
The computer must be started with a user having ‘
Administrator’ privileges to be able to install software
or change the camera on Windows XP.
LAS V3.2 or earlier versions do not have to be
uninstalled before installing LAS V3.3 but this is
recommended. After installing LAS V3.3, it is not
possible to revert to LAS V3.2.
Continued...
Before installing LAS V3.3, we recommend that a
backup is made of any existing archives using the
LAS V3.2 Backup tool.
Do not delete archives before installing V3.3 as
they can only be imported into V3.3 if they are left in
an unchanged state.
LAS Installation Guide
1-6
LAS Installation Guide: Installing with Windows XP (continued):
Close any other programs that are
running. The best way of ensuring this
is to re-boot the computer.
1: Place the DVD drive. Usually, the
start up screen will appear
automatically.
2: Click on the Install Leica
Application Suite and DFC Twain
button.
3: If the program does not start
automatically, navigate to the DVD
drive in Windows Explorer and
double-click it.
4: From the list of files on the DVD,
double-click the
LeicaApplicationSuite.exe file and
the start up screen will appear.
Click on the Install Leica
Application Suite button (2).
Follow the instructions that appear on
screen. Normally it is only necessary to
select the default options.
Some required Windows
components may be installed before
LAS, typically .Net and DirectX.
These can take several minutes so
please be patient.
After .Net has been installed, the PC
must be rebooted. After the PC boot
process is finished the LAS
installation process must be
restarted. Go back to Step 1 on this
page. This time the .Net files are
already loaded so the reboot will not
occur and the installation will
proceed.
1-7
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Sentinel Protection Installer Warning
Please note that if you are installing
LAS V3.3 on a PC that already has
Leica MM AF installed, then the
message shown may appear during
installation.
Please ignore this advice as LAS V3.3
is compatible with the newer version of
the Sentinel Protection software that is
detected.
Do NOT remove the newer version,
simply click OK and continue.
LAS Installation Guide
1-8
LAS Installation Guide: Minumum and Custom Setup: XP:
If Minimum Setup is selected during
installation, no optional modules will be
installed and LAS can be used in the
LAS Core mode for use with LAS Image
Explorer (Folders). This installation
provides the simplest operation and do
not require a license file.
If Custom Setup is selected during
installation, the optional modules may
be chosen individually. Select only
those that are required immediately;
others can be added later (1).
By default, the Leica DFC camera
drivers are installed in the current
operating system language. It is not
necessary to specifically select ‘Install
DFC Drivers’ from the start up screen.
This option is provided for users who do
not wish to install LAS but do want to
use the cameras with another program.
Make sure that any Leica DFC
camera is disconnected whilst installing
the DFC Drivers. If the camera is
connected, the installation may fail
preventing image capture or colour loss.
To install Leica EZ4D first complete
the LAS installation and then click Install
EZ4D on the Documents and Drivers
screen. Then follow the screen
instructions.
1-9
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: MultiTime Movie: XP:
If the LAS MultiTime Movie module is
installed with Windows XP(SP2), it may
be necessary to install a Microsoft
Security Update. A warning appears (1)
but do not reboot the system if asked to
do so.
If the Security Update (2) is not
available for the language of your
Windows XP, then please download it
from the Microsoft download centre.
Find this by searching for the article
KB904706.
See Release Notes under
Restrictions: MultiTime for further
advice.
When module installation is complete
the start up screen appears again. Click
on the Exit button to complete the
installation.
The fonts used by LAS require the Clear
Type option to be enabled. Right-click
the computer Desktop and…
3: …select Properties from the menu.
4: On the dialog select Appearance
and then Effects (5).
6: Enable the Use the following
methods to smooth edges of screen
fonts check box.
7: Click on the arrow to the right of the
list box and from the drop down
select Clear Type.
8: Click OK. Restart the computer.
LAS Installation Guide
1-10
LAS Installation Guide: Connecting the Camera and Microscope: XP:
Connecting the Camera and
Microscope:
Connect the FireWire cable to the
computer and the Leica DFC camera.
1: The first time a camera is used, the‘
Welcome to the Found New
Hardware Wizard will appear.
2: Select the No, not this time option
to prevent connecting to the
Internet to find a camera driver
software.
3: Click Next and follow the
instructions.
Connect the Leica microscope cable to
the computer. The first time a USB
interfaced microscope is used, the
Welcome to the Found New Hardware
Wizard will appear. Select the No, not
this time option to prevent connecting to
the Internet to find a camera driver
software.
Click Next and follow the instructions.
Go to Selecting Hardware.
1-11
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Installing with Windows Vista:
Installation Steps: Windows Vista
only:
Install the microscope following the
instructions provided.
· Do NOT connect the interface
cable between the microscope
and the computer.
· Do NOT connect the FireWire
cable to the Leica DFC camera
until the software has been
installed.
· Disconnect the computer from a
network during installation to
prevent accidental loading of
drivers from the Internet if an
incorrect option is chosen. All LAS
microscope and camera drivers
are on the supplied LAS DVD.
Close any other programs that are
running. The best way of ensuring this
is to re-boot the computer.
1: Place the LAS DVD in the drive.
Usually, the start up screen will
appear automatically.
2: Click on the Install Leica
Application Suite and DFC Twain
button.
3: If the program does not start
automatically, navigate to the DVD
drive in Windows Explorer and
double-click it.
4: From the list of files on the DVD,
double-click the
LeicaApplicationSuite.exe file and
the start up screen will appear.
Click on the Install Leica
Application Suite button (2).
LAS Installation Guide
1-12
LAS Installation Guide: Enhanced Security Features: Vista:
The Windows Vista operating system
includes enhanced security features.
Extra confirmation dialogs occur during
the installation and you must run LAS in
Administrator Mode to modify its
licence.
You must have full Windows
Administrator privileges to install LAS
software. In a corporate network
environment, it may be necessary to
obtain assistance from the customer’s
IT department.
After a short delay, the screen
darkens and a Windows security dialog
appears. This dialog blocks the system
until it is dismissed, so it’s not possible
to provide a screenshot. The dialog is
called User Account Control and warns
An unidentified program wants access
to your computer.
· Check that the program is called:
vcredist_x86_ENU.exe
…then select Allow.
· The User Account Control dialog
appears again. Check the
program concerned is:
Setup.exe
…then select Allow.
1: If DFC Twain is not installed, the
dialog appears.
2: Click Install.
If Vista warns that it cannot verify
the publisher of the driver software.
Select Install this driver anyway and
after it has installed click Finish.
Follow the instructions that appear on
screen for the remainder of the
installation. Normally it is only
necessary to select the default options.
Some required Windows components
may be installed before LAS, typically .
NET and DirectX.
After .Net has been installed, the PC
must be rebooted. After the PC boot
process is finished the LAS
installation process must be
restarted. Go back to Step 1 on this
page. This time the .Net files are
already loaded so the reboot will not
occur and the installation will
proceed.
1-13
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Minimum and Custom Setup: Vista:
If Minimum Setup is selected during
installation, no optional modules will be
installed and LAS can be used in the
LAS Core mode for use with LAS Image
Explorer (Folders). This installation
provides the simplest operation and do
not require a license file.
If Custom Setup is selected during
installation, the optional modules may
be chosen individually. Select only
those that are required immediately;
others can be added later (1).
By default, the Leica DFC camera
drivers are installed in the current
operating system language. It is not
necessary to specifically select ‘Install
DFC Drivers’ from the start up screen.
This option is provided for users who do
not wish to install LAS but do want to
use the cameras with another program.
Make sure that any Leica DFC
camera is disconnected whilst installing
the DFC Drivers. If the camera is
connected, the installation may fail
preventing image capture or colour loss.
To install Leica EZ4D: First complete
the LAS installation and then click Install
EZ4D on the Documents and Drivers
screen. Then follow the screen
instructions.
LAS Installation Guide
1-14
LAS Installation Guide: Vista Aero:
When module installation is complete
the start up screen appears again. Click
on the Exit button to complete the
installation.
Windows Vista Aero:
Aero is the type of graphics used by
Vista. Some graphics cards do not
perform as expected when Aero is
running. For example the sliders bars
do not update properly. If this is the
case:
1: Use Windows Vista Basic graphics
instead of Aero.
Restart the computer.
1-15
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Connecting Camera and Microscope: Vista:
Connecting the Camera and
Microscope:
Connect the FireWire cable to the
computer and the Leica DFC camera.
Windows Vista recognises the camera
immediately and there is no New
Hardware wizard.
Connect the Leica microscope cable
to the computer. The first time a USB
interfaced microscope is used, the
Welcome to the Found New Hardware
Wizard (1) will appear.
2: Select the No, not this time option
to prevent connecting to the
Internet to find a camera driver
software.
3: Click Next and follow the
instructions.
Go to Selecting Hardware in the next
section.
LAS Installation Guide
1-16
LAS Installation Guide: Selecting Hardware:
Selecting Hardware:
The first time Leica Application Suite
runs, the microscope and camera
models and settings have to be set up
and to do this it must be started from an
account with Administrator privileges.
If a Desktop icon for LAS is not present,
refer to the next page.
Starting LAS: Windows XP:
If the current account has Administrator
privileges, double-click the left mouse
button on the Desktop icon created
during the installation and LAS will start
normally.
For Limited User accounts:
1: Right click the LAS Desktop icon.
2: Select Run as… from the drop
down menu.
3: Select a user with Administrator
privileges, enter the password and
click OK (4). LAS will start.
Starting LAS: Windows Vista:
Double clicking the left mouse button on
the Desktop icon will not launch LAS
even if Administrator privileges are
current, so for a first-time run:
1: Right click the LAS Desktop icon.
2: Select Run As Admin… from the
drop down menu.
3: Complete the Administrator
credentials and click OK (4). LAS
will start.
Continued...
1-17
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Selecting Hardware (continued):
If a Desktop icon for LAS is not present,
it can be launched from the computer
program list. Administrator privileges
are still required.
1: Position the cursor over the
Start>All Programs button.
2: Navigate to the Leica Application
Suite V3 entry and position the
cursor over it. The Leica menu
appears.
3: Locate the Leica Application Suite
item and right click it.
4: From the menu click to select Run
as…
5: Complete the dialog, selecting a
user with Administrator privileges.
Click OK (6). The program will run.
LAS Installation Guide
1-18
LAS Installation Guide: Selecting Microscope:
If you want to setup the microscope and
camera, start LAS in the 'framework' or
licensing mode. Do this by selecting
License LAS from the Start menu group
for LAS. In this mode you can change
the Hardware Setup, make a
microscope Firmware Update and
perform registration tasks.
1: Select Options on the main header
and...
2: …from the drop down menu select
Hardware Setup by clicking it.
3: Click on the arrows to the right of
the Microscope window. The
microscope selection list appears.
4: Use the up/down arrows to scroll
through the list.
5: Click on the required microscope.
The list will close and the
microscope selected will appear in
the window.
Continued...
1-19
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Selecting Microscope (continued):
The options and accessories available
depend upon the microscope chosen.
Automatic and motorised models will
certainly have a computer connection
option – in the example RS232 COM
ports and USB (Universal Serial Bus)
options are shown. The list should be
checked against the microscope
manual.
Some microscopes will have an Action
Logging facility which can be viewed by:
1: Clicking on the arrow to the right of
the microscope selection window. If
Logging is not available the arrow
will be appear dimmed.
2: Use the buttons on the Microscope
Advanced Settings dialog to view or
clear the log. Logging will occur only
if the Log Communication Traffic
checkbox is checked.
To select the connection type:
3: Click on the arrows to the right of
the Connection window.
4: Use the up/down arrows to scroll
through the list. Select an available
connection by clicking it.
Selecting accessories:
In this example a motorised stage is an
option (although may not be fitted).
5: Click on the arrows to the right of
the Accessory (Stage-Controller)
window and from the list choose…
6: …None if the accessory is not fitted
or…
Select the appropriate accessory.
Enabling other accessories may
also reveal other options. Check
the manual and the microscope’s
delivery manifest before making
selections.
7: Click the Test button. This will
perform a connection integrity test.
Incorrect or faulty connections will
result in an error message.
LAS Installation Guide
1-20
LAS Installation Guide: Selecting Camera:
A few microscope models do not have a
camera option so there will not be an
image source (= camera) list. For those
microscopes that may be fitted with a
camera:
1: Click on the arrows to the right of
the Image Source window.
2: Use the up/down arrows to scroll
through the list.
3: If a camera is fitted to the
microscope, find the model type on
the list and click to select it.
For microscopes without a camera,
select the Demo Camera or None
option.
4: Click Save to save the microscope
and camera settings and exit.
1-21
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Optional Modules (Evaluation):
Optional Modules are available for a 60
day free evaluation period. After that a
licence has to be purchased for their
continued use.
Do not apply for a license
unless you have
purchased any optional
modules.
When Leica Application Suite is
installed most of the modules are also
installed but remain disabled until either
Demo mode is started and module(s)
are enabled, or a module is purchased
and licensed.
Optional modules are started in
Demo mode by clicking the Demo
button in the Start Demo column. Both
licensed and demonstration modules
can run together simultaneously.
The 60 day evaluation period begins
as soon as a module is enabled in
Demo mode and cannot be stopped by
disabling the module. Demo mode may
be deferred until the user is ready to
evaluate the modules.
To start the evaluation period:
1: Click on Options on the tool bar.
2: From the drop down menu, choose
Registration Info.
3: On the Licence Status dialog a
check mark indicates those modules
that are enabled. Click to enable or
disable the required modules.
4: The Enable All button will enable all
of the modules for Demo and (5)
the Disable All button will clear all
modules.
Continued...
LAS Installation Guide
1-22
LAS Installation Guide: Optional Modules (Evaluation) continued:
6: To start a module in Demo mode,
click the button in the Start Demo
column. The enable/disable check
box is automatically checked.
7: Clicking the Start All button will
enable all of the modules and start
the evaluation period. A
confirmation warning is displayed
because this action cannot be
undone.
8: Click Close.
9: The Status dialog can be checked
at any time. Use the drop down to
select an appropriate status filter.
With the modules selected the next
step is to licence the Demo period.
1-23
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Optional Modules (Demo Licence):
With the required modules selected on
the Registration Info: Licence Status
dialog, the Demo licence has to be
installed for the modules to become
enabled and active:
1: Click on Options on the header bar
and…
2: …select Registration.
The DVD supplied with the Leica
Application Suite must be in the
computer drive.
3: Click on the Install demo licence
button. The Demo licence number
will be automatically retrieved from
the DVD and installed on the
machine.
4: Click on the Start Demo button and
the Demo period for the selected
modules will start.
5: Click Close.
LAS Installation Guide
1-24
LAS Installation Guide: Optional Modules (Licensing):
Optional Modules are purchased
through local Leica Sales centres which
will provide a Module Licence Number
when the sale is complete. A single
Module Licence Number can relate to
one or several modules.
The user sends the Module Licence
Number to the Leica Configuration
Centre either by e-mail or by fax. They
return a Licence file either as an e-mail
attachment, or a CD. The Licence file
provides a code which, when entered
into the computer will allow Optional
Modules to perform normally after the
60 day evaluation period.
1: Position the cursor over the
Start>All Programs button.
2: Navigate to the Leica Application
Suite V3 entry and position the
cursor over it. The Leica menu
appears.
3: Locate the Licence LAS item and
right click it.
4: From the menu click to select Run
as…
5: Complete the dialog, selecting a
user with Administrator privileges.
Click OK (6). The program will run.
Continued...
1-25
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Optional Modules (Licensing) continued:
To obtain a Licence file:
1: Click on Options on the main
header.
2: Select Registration from the drop
down menu.
3: On the Select Licence File dialog
the Site Code is generated
automatically either from a single
user machine or from a network. If a
coded Dongle has been purchased
it will have to be in a USB port every
time LAS is running. Fit the Dongle
and click the Use Dongle check box
(3). The site code is different for
Dongle users.
4: Click the Register button and…
5: …on the Registration Form
complete the Contact Details. There
is a drop down menu from which to
select a country name.
6: Enter the number printed on the
software packing.
! Keep the packaging in a safe
place.
Dongle users go to the next page
item (7).
7: Click on the Save button.
Continued...
LAS Installation Guide
1-26
LAS Installation Guide: Optional Modules (Licensing) continued 2:
With the Registration Form complete
and the Save button clicked, the form
details have to be saved prior to either
e-mailing or faxing to Leica.
1: The Windows Save As dialog
appears. Navigate to a folder in
which to save the Registration
Form.
2: Type a name for the form and…
3: …click Open.
Send the Registration Form as an
attachment to an e-mail or as a fax.
The e-mail address and fax number
are shown on the top of the
Registration Form (4).
Leica will respond with either an
e-mail or CD containing the Licence
file.
Start the licensing procedure
(please see Running the
Framework etc.) and select
Registration from Options:
E-mail users:
On the returned e-mail, click on the
licensing attachment and save it to
an appropriate location on the hard
drive. The location is unimportant
but the licence file name must not
be altered.
All users:
Back on the Select Licence File
dialog, click the Browse for Licence
button (5) and on the Windows
Navigator locate the Licence Key
file and click to select it. CD users
should navigate to the CD, and
select Explore to locate the file.The
file name and location (path) will
appear in the Licence File widow
(6).
7: Click the Validate Licence
button. If the file is valid the
appropriate optional modules will
usable and free from any
restrictions. An invalid key file will
result in an error message. Contact
Leica for further assistance.
Dongle users do not have to apply
for a file - it is supplied on the CD
that came with the dongle.
1-27
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Installing Power Mosaic Hardware:
LAS Power Mosaic requires an Oasis-blue Stage
Controller board (12 730 193) and the cables
supplied with the board. For details see System
Requirements.pdf on the LAS Installation DVD..
Oasis-blue is a universal PCI board designed to
directly control and drive stage and focus stepper
motors of the two or four phase variety, which are
suitable for micro-stepping. The board does not
require additional power supply or amplifiers.
After the board is installed, Leica Application Suite
controls all of the Oasis functions.
For use with an IsoPro stage, please refer to the
additional notes that are in the root folder of the LAS
DVD.
Computer Requirements:
· The computer must conform to the specification
detailed in System Requirements and:
· Have one half-length PCI or PCI-X slot available
that is not obstructed by an adjacent board.
· Windows operating system compatible with
LAS.
· One free power connector (4 pin hard drive) with
2A at 12V available.
· Cross-head screwdriver.
· LAS software disk.
LAS Installation Guide
Hardware Installation Procedure
· Switch off the microscope and unplug the
electrical supply.
· Switch off the PC and unplug the electrical
supply to disable any standby power.
· If the microscope has a CtrMic box, connect the
microscope Z-breakout cable between the ‘
Microscope’ connector on the rear of the CtrMic
box and the equivalent connector on the
microscope.
· Remove the computer casing cover or side
panel. Select a PCI slot with no obstructions
(preferably one with adequate air-flow from the
auxiliary fan), and remove the rear blanking
panel.
NOTE: The Oasis drive software must be installed
from the DVD and configured before starting
LAS and using Power Mosaic.
1-28
LAS Installation Guide: Oasis-blue Board:
Important: For safety reasons always
use an antistatic mat or other
grounded source to discharge static
electricity before handling the board.
Pick up the board (still in its anti-static
sleeve), by grasping the metal edge
bracket with one hand, and remove the
sleeve.
If the microscope has a CtrMic box:
1: Connect the pass-through ribbon
cable to PL2 on the rear of the
Oasis-blue board.
2: Fit the MIC Pass-through plate
holding the 15-way D-type
connector at other end of this
ribbon cable into a free access slot
on the rear of the PC.
Holding the edges of the board
only, position it over the PCI slot
and locate the tip of the metal
bracket in the access slot of the PC
chassis, before pushing the board
firmly but gently home with a slight
rocking action.
Secure the bracket with a retaining
screw or clip as necessary.
3: Connect the spare power
connector from the PC power
supply to PL1 at the rear of the
board. The Oasis-blue must be
the only device drawing power
from this lead. The on-board
motor drive components get their
power via the +12V from this
connector. There is an extension
cable provided with the
Oasis-blue in case the power
cable is too short.
Refit the computer cover.
Continued...
1-29
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Oasis-blue Board (continued):
All three connectors on the Oasis-blue
are identical and are not keyed, so care
must be taken to fit the cables correctly.
If they are not, the Oasis-blue software
will not work at all.
4: Connect a Leica SmartMove or
joystick or other compatible control
device to SK1 (marked I/O on front
panel) as required.
5: Connect the Focus/Trigger cable to
SK2 (marked Z/F).
6: Connect the Focus cable to the
focus motor connector. If the
microscope uses a Leica CtrMic
box, the focus connector is on the
CtrMic microscope Z-breakout
cable. Otherwise it is on the
external focus motor.
7: Connect the trigger cable to the
Leica DFC 4-pin trigger port on the
rear of the camera. This cable is
required only for DFC FX cameras
or DFC400 and must not be
connected for DFC290 or DFC420.
8: Connect the XY stage cable to SK3
(marked X/Y on front panel) and to
the connectors on the stepper
motor stage.
If a CtrMic box is being used,
connect the SmartMove pass
thorough cable to the D-type
connector and the other end to the
CtrMic where the SmartMove would
normally be connected.
Switch on the PC and microscope
to proceed with the driver
installation for your operating
system.
LAS Installation Guide
1-30
LAS Installation Guide: Power Mosaic with Windows XP:
If the Oasis drivers have not been installed during the
LAS installation, please follow this procedure to install
them.
· Switch on PC and boot into Windows XP.
· Run the program \Oasis\Setup.exe on the LAS
disk.
· Click on the Install Oasis Controller Device Driver
option. A message will indicate the drivers were
installed and registered correctly.
· The Add New Hardware Wizard should appear.
· Insert the LAS disk and select Search
automatically for best driver for your device and
click Next
· The installation wizard should detect the
Oasis-blue controller and install the relevant
files.
· Click on the Install Utilities option to install the
Configuration Wizard, the Flash Configuration
program and the Oasis.exe utility.
· Run the Oasis.exe utility program to check the
Oasis-blue is functioning properly
· After drivers and files have been copied to the
relevant directories, click Finish.
1-31
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Oasis-blue with Other Hardware:
Run the Oasis Configuration Wizard from its icon and · The stage limit switch polarity and orientation will be
follow the on-screen instructions. Some parts of the
automatically noted and programmed into the
process take a few seconds, so please be patient after
Oasis-blue Flash memory, so that it is not
clicking the Next button; otherwise you may skip past
necessary to repeat this process unless the stage
an important dialog.
type is changed.
The Wizard screens showing typical settings are
shown on the following pages.
· The preferred direction of travel for each axis can
Check that lenses and cables are well clear of the
be set using the wizard, to suit the hardware
stage because the Auto-detect XY section will move it
configuration. The defaults generally suit a
to its limit switches.
microscope with the camera oriented in such a way
that the view on the monitor is the same as the view
· Selecting an XY stage type indicates the size of
through the eyepieces, and with a motorised focus
the stage and calculates the lead-screw pitch adapter mounted on the left hand side.
how far the stage moves for one turn of an axis
motor. If the stage type is not properly specified, · The Axis pitch setting in the focus dialog should be
or is not present in the list, the lead-screw pitch
set to the distance Z is raised or lowered for one
may be calculated incorrectly. In this case it will
turn of the motor. This is usually one turn of the
be necessary to key in a value. Consult the
microscope fine focus knob normally calibrated in
information provided by the stage manufacturer.
microns (typically 100 microns per revolution) so
enter 0.1mm. For a Leica DM microscope with
internal focus motor enter 0.78 mm.
Continued...
LAS Installation Guide
1-32
LAS Installation Guide: Oasis-blue with Other Hardware (continued):
1-33
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Oasis-blue with Other Hardware (continued 2):
LAS Installation Guide
1-34
LAS Installation Guide: Oasis-blue with Other Hardware (continued 3):
1-35
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Check Oasis-blue Settings:
The Oasis utility software can check if
the board is working properly. The most
useful screens are illustrated here:
1: Stage X/Y Settings.
2: Focus (Z) Drive Settings.
If the focus (Z) drive limit switches on a
DM microscope are configured
incorrectly, when a limit is reached, it
will not be possible to move away from
it. It will appear as though the focus
drive is stuck despite the ‘clicking’ as
the from the limit switch as the motor
presses against it.
The solution is to reverse the limits by
opening the Oasis Status dialog by
clicking on Show Status button (1 and
2).
Continued...
LAS Installation Guide
1-36
LAS Installation Guide: Check Oasis-blue Settings (continued):
3: Set the User Neg Set and User Pos
Set to the values shown in the Z
column.
If an inverted microscope is used,
these values will need to be
inverted due to the direction of the
stage Z movement.
1-37
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Installing Stage with Encoders:
The 12 730 196 Märzhäuser Scanning
Stage, 100x80mm, 2mm with Encoders,
requires the 12 730 195 Oasis-blue
Encoder kit for Power Mosaic to be
fitted to the Oasis-blue board.
1: The Oasis-blue Encoder is a
daughter board that fits on the
Oasis-Blue board as shown in the
illustration:
2: A modified XY Cable, that carries
the Encoder signals, is connected
between the XY connector on the
Oasis-blue board and the stage.
Run the Oasis Configuration
Wizard from its icon, select the
Encoder Setup Wizard and follow
the steps shown on the following
pages.
Continued...
LAS Installation Guide
1-38
LAS Installation Guide: Installing Stage with Encoders (continued):
1: The Oasis Application will show the
Encoder settings as illustrated:
2: For LAS Power Mosaic these
properties will appear as shown.
Note the setting for Encoder
accuracy. If narrow white lines
appear between mosaic tiles when
the encoders are enabled, use the
Auto Correct facility to adjust the
stage position. The number of
microsteps to enter depends on the
accuracy of the stage, but an initial
value of 4 can be subsequently
adjusted to suit the target system
1-39
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Installing Stage with Encoders (continued 2):
LAS Installation Guide
1-40
LAS Installation Guide: Installing Stage with Encoders (continued 3):
1-41
© 2008 Leica Microsystems (Switzerland) Ltd
LAS Installation Guide: Further Support:
Your purchase also gives you access to the Leica
Application Suite e-mail Hotline in case of technical
questions:
DI.support@leica-microsystems.com
or
MQM-Hotline@leica-microsystems.com
LAS Installation Guide
When using the Hotline please mention the exact
configuration you are working with and your software
version/release. You will find this information by
selecting the About command from the Help menu.
This will facilitate the analysis of your query and
accelerate our response time.
1-42
Leica Microsystems (Switzerland) Ltd.
Stereo and Macroscope Systems
CH 9435 Heerbrugg
Switzerland
Telephone: +44 1223 411411
FAX +44 1223 412526
Hotline: +44 1223 401824
di.support@leica-microsystems.com
www.microscopy-imaging.com
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