Safe operation of your QIAcube

Safe operation of your QIAcube
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TechnicalInformation
Safe operation of your QIAcube®
This Technical Information describes some critical issues that you should be aware of when operating the QIAcube. Only use
the consumables that are described in this document. Use of other consumables will result in load check errors, pipetting
failures, reduced application performance, and centrifuge crashes. When starting a protocol, ensure that the correct consumables
are used in the correct worktable positions, as outlined in the corresponding QIAcube Protocol Sheet. Be aware that the
consumables required may change depending on the application.
IMPORTANT: Damage caused to the QIAcube by using spin columns or other plastic materials that
are not recommended by QIAGEN will void your warranty.
Sample Tubes RB (2 ml), cat. no. 990381
For more information, see the “Loading the samples” section of the QIAcube User Manual.
Ensure that you place the lids of the tubes in the corresponding slots of the
2,5
1,5
1,0
shaker rack.
Only use with shaker adapter “2”.
0,5
Sample Tubes CB (2 ml), cat. no. 990382
For more information, see the “Loading the samples” section of the QIAcube User Manual.
Only use with shaker adapter “S2”.
On the shaker, always use in combination with shaker rack plugs.
Do not use any other type of conical-bottomed tube.
Processing Tubes (2 ml)
For more information, refer to the corresponding QIAcube Protocol Sheet or Kit Handbook.
Only use with shaker adapter “2”.
On the shaker, always use in combination with shaker rack plugs.
Only use Processing Tubes (2 ml) that are supplied with QIAGEN spin-column kits.
Microcentrifuge tubes (1.5 ml)
For more information, see the “Internal features of the QIAcube” section of the QIAcube User Manual.
Always use 1.5 ml microcentrifuge tubes. These tubes are supplied with the Starter
Pack, QIAcube (cat. no. 990395) and Rotor Adapters (10 x 24) (cat. no. 990394).
1,5
Do not use any other type of 1.5 ml tube.
1,0
0,5
Do not use 1.5 ml microcentrifuge tubes on the QIAcube shaker for sample
preparation.
Sample & Assay Technologies
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Rotor Adapters (10 x 24), cat. no. 990394
For more information, see the “Loading the rotor adapters” section of the QIAcube User Manual.
Ensure that spin columns and tubes are placed in the correct positions
(1–3) of the Rotor Adapter.
Ensure that the lids of the spin columns are securely placed in slots
L1
2
1
L2
L1–L3 of the Rotor Adapter, as indicated in the QIAcube Protocol Sheet.
L3
Ensure that the lid of any spin column that is placed in position 2 of
3
the Rotor Adapter is carefully cut off. No part of the lid should remain.
Do not use damaged Rotor Adapters.
Do not reuse Rotor Adapters.
Special buffer positions A, B, and C
For more information, refer to the corresponding QIAcube Protocol Sheets.
Always use the tubes that are indicated in the corresponding QIAcube
Protocol Sheet.
Always load the tubes with the exact volume of buffer, depending on
A
the number of samples indicated on the QIAcube Protocol Sheet.
B
If positions A, B, and C are used at the same time, carefully cut off
C
the lid of one of the tubes. Place the remaining 2 lids into the
corresponding slots.
Do not fill the tubes with too much or too little buffer.
Rotor nut
For more information, see the “Installing the QIAcube” and “Packing the QIAcube” sections of the
QIAcube User Manual.
Always tighten the rotor nut when installing the rotor in the centrifuge.
To do this, use the rotor key that is supplied with the QIAcube.
If the rotor nut is not tightened properly there is a risk of personal injury
or material damage.
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual.
QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical
Services or your local distributor.
Trademarks: QIAGEN®, QIAcube® (QIAGEN Group).
1057255 05/2009
© 2009 QIAGEN, all rights reserved.
www.qiagen.com
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Sample & Assay Technologies
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