Solid Phase Extraction Solutions

Solid Phase Extraction Solutions
SPE
Solid Phase Extraction Solutions
Introduction
All of Gilson’s SPE and Liquid
Gilson offers the broadest array of SPE instruments on the market today, including the
Handling instruments are controlled
GX-271 ASPEC , the GX-274 ASPEC , the SPE 215, and virtually any other Gilson instrument
via Trilution™ LH software.
that accommodates the Code 300 Series racks.
Trilution LH has been designed
™
™
with you, the user, in mind. The
Fundamental to Gilson’s automated Solid Phase Extraction (SPE) system is our mobile rack and positive
graphical interfaces decrease the
pressure technology. The mobile rack technology allows you to automate your entire SPE and Liquid
learning curve while increasing
Handling processes from the initial condition to the final transfer. Gilson’s SPE instrumentation utilizes
your efficiency. Trilution LH allows
positive pressure to ensure more reproducible flow rates, more reproducible recoveries, less sample to
you to concentrate on the science,
sample contamination, and higher precision.
not the software.
Trilution™ LH provides an intuitive interface for creating SPE and Liquid Handling protocols /
As is the case with all Gilson
applications to condition cartridges, load the sample, wash the cartridge, elute the analyte of interest
and transfer the eluent to a final sample vessel or inject it directly onto an analysis system.
Available as a single probe,
SPE 215
instruments, the SPE and Liquid
Handling instruments are extremely
versatile, modular, and robust. Gilson’s SPE instruments are versatile enough to adapt as your science changes and
multiple probe, or spannable
robust enough to guarantee a low cost of ownership and a workhorse instrument for years to come.
multiple probe instrument Gilson
has a SPE and Liquid Handling
instrument solution to fit your needs.
Solid Phase Extraction System Features . . . . . . . . . . 1–2
GX-271 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 3–4
GX-274 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 5–6
SPE 215 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7–8
Trilution™ LH . . . . . . . . . . . . . . . . . . . . . . . . . 9–10
Code 300 Series Racks . . . . . . . . . . . . . . . . . . . . . 11
Automating SPE . . . . . . . . . . . . . . . . . . . . . . . . 12
GX-271 ASPEC
i
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Solid Phase Extraction Solutions
ii
Solid Phase Extraction System Features
Pressure is constantly monitored and recorded
through a pressure sensing module to immediately
Optional peripherals such as injectors,
identify a change in performance, cartridge failure,
on-bed solvent reservoirs, 3-way valves and
or plugged solvent lines.
peristaltic pumps extend the capabilities of
your instrument and allow you to tailor the
instrument to meet your specific needs.
Complete control via Trilution™ LH, Gilson’s newest
and most powerful liquid handling software yet.
The 406 Single and Dual Pumps deliver
Mobile rack
positive pressure via a gas cylinder or to
technology allows
achieve more accurate flow rates use the
the user to perform
syringes for the air push.
typical Liquid
Handling tasks as
well as SPE tasks
Compatible with the most common and
all from the same
widely accepted SPE cartridges and
bed layout.
96-well extraction plates. The Gilson
instruments are capable of utilizing
Positive Pressure
1, 3, and 6 mL SPE cartridges as well
elutions guarantee
as 96-well extraction plates.
more reproducible
results, higher
percent recoveries,
On-bed rinse stations minimize
and a more
carryover and contamination concerns.
consistent flow rate.
GX-274 ASPEC with
two 406 Dual Syringe Pumps
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Solid Phase Extraction Solutions
GX-271 ASPEC™
6mL
3mL
1mL
Automated Solid Phase Extraction, Liquid Handling and
On-line Injection capabilities
Whether your application
is pharmaceutical,
clinical, forensic, or
environmental. SPE has
become the technique
of choice for sample
cleanup, sample prep,
System Overview
Cartridge Types
1, 3, 6 mL Cartridges
Air Push Modes
Syringe or Gas Cylinder Valve
On Bed Options
Reservoirs, Direct Injection Module
Dimensions (w x d x h)
59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in)
in both batch and sequential mode to increase
° Operate
throughput and efficiency.
n-bed reservoirs allow access up to four solvents
° O
without taking up a valuable rack position
° Accommodates 1, 3 and 6 mL SPE cartridges.
Cartridge Capacities*108–1 mL cartridges
60–3 mL cartridges
45–6 mL cartridges
° Mobile Rack Technology enables use as both a
SPE instrument as well as a Liquid Handling instrument.
*Assumes 3 tray positions used for SPE mobile racks.
° Multiple rinse stations with optional rinse pump
allows multiple rinsing parameters depending on
application need.
Designed with today’s laboratory in mind, the GX instruments
have large cartridge capacities while minimizing the amount of
° The 406 Single Syringe
Pump is equipped with a
pressure sensing module
that monitors pressure
and can be used to handle
error conditions such as
high cartridge pressure, or
clogged liquid lines.
linear bench space consumed. System performance is constantly
and trace analyte
enrichment because of
its inherent versatility,
selectivity, speed and
low solvent usage.
monitored by a pressure sensing modules. Carryover and
contamination concerns are minimized through the use of Gilson’s
GX-271 ASPEC with on bed solvent reservoirs
revolutionary Jet Wash rinse stations. Combining the throughput
and ability to run unattended makes this instrument an ideal choice
for SPE, sample prep,
sample clean up and
GPC / SPE applications.
406 Syringe Pump
On Bed Injection Capabilities
06 Single Syringe Pump
° 4
enables air pushes from
both syringes and external
air sources.
product can be fractionated by moving to
° Eluted
different collection tubes for applications such as
buffer or pH step gradients.
° GX Direct Injection Module can be integrated
for performing on-line injections onto HPLC or
LC/MS systems.
° Positive pressure achieved via Gilson’s unique and
innovative sealing cap design.
° Modular design allows the use of Code 20, Code 200,
and Code 300 series racks.
Mobile racks allow access to both SPE
cartridges as well as collection vials
GX-271 ASPEC
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Solid Phase Extraction Solutions
GX-274 ASPEC™
6mL
3mL
1mL
Increased throughput through parallel processing
System Overview
Maximize your efficiency and
Cartridge Types
1, 3, 6 mL Cartridges
Air Push Modes
Syringe or Gas Cylinder Valve
On Bed OptionsReservoirs, Four Probe Off-Bed Solvent Transfer Port
throughput by unattended,
Dimensions (w x d x h)
59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in)
Cartridge Capacities*108–1 mL cartridges
60–3 mL cartridges
45–6 mL cartridges
parallel processing
*Assumes 3 tray positions used for SPE mobile racks.
of samples. Gilson’s
multiple-probe instruments
° Four probe Z-arm increases throughput and efficiency.
° On-bed reservoirs allow access up to four solvents without taking up
a position on the tray.
give you this ability.
° On
bed transfer ports allows access
to 2 additional large volume solvent
reservoirs without taking up any
on bed rack positions. Extend this
capability through the use of a
VALVEMATE® II.
1. Place the samples on the
instrument, SPE cartridges
in the mobile racks, and
fill any solvent reservoirs.
VALVEMATE® II Valve Actuator
2. Create your sample list
The GX-274 ASPEC is a high throughput four-probe instrument capable
3. Press Start...
its that easy.
Collection vessel access
of processing up to 4 samples in parallel increasing both throughput
and efficiency of your cartridge-based applications. Based on positive
pressure elutions this instrument processes up to four samples in
design allows the use
° Modular
of Code 20, Code 200, and
Code 300 series racks.
406 Dual Syringe Pumps are equipped with four independents
° The
pressure sensing modules that monitor pressure on each of the four
fluid paths and can be used to handle
error conditions such as high cartridge
pressure, or clogged liquid lines.
parallel with an innovative sealing cap technology, where each cartridge
is sealed, run, and monitored individually. The two 406 Dual Syringe
Pumps allow the user to transfer liquids, process up to four samples in
parallel, and to monitor each fluid path individually.
° Accommodates 1, 3 and 6 mL
SPE cartridges.
Process four samples in parallel
Rack Technology enables use
° Mobile
as both a SPE instrument as well as
a Liquid Handling instrument.
pressure elution achieved via
° Positive
Gilson’s sealing cap technology.
° Positive pressure elution
ensures better reproducibility
and higher precision.
° Eluted product can be fractionated by
moving to different collection tubes.
° SPE process can be performed in
either batch and sequential mode to
increase throughput and efficiency.
GX-274 ASPEC
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Solid Phase Extraction Solutions
SPE 215 System
3mL
1mL
96 well extraction plates
High-Throughput Solid Phase Extraction and Sample Purification
What else can you do with
your Gilson SPE system?
Due to Gilson’s modular
The SPE 215 System is an easy-to-use, powerful, sample
preparation solution for your laboratory. This instrument uses
Gilson’s Integrated Sealing foot design to achieve it’s positive
pressure elutions. Gilson’s multiple probe technology provides
the high throughput capability and the ability to increase
design you have the ability
to match your instrument
to your exact needs.
Peripherals such as
and improve your laboratory’s output and results. Dual Rail™
technology provides accurate and even pressure across all
eight syringes allowing for the selection of one to eight probes.
The integration of these technologies provides you with a
solid SPE solution and results in increased productivity and
efficiency in your laboratory.
° Mobile Rack Technology enables use
as both a SPE instrument as well as a
Liquid Handling instrument.
ccommodates 1 and 3 mL cartridges or
° A
96-well extraction plates.
° Can be operated in both batch and sequential
mode to increase throughput and efficiency.
° Four or Eight probe configurations to
increase throughput and efficiency.
° Positive pressure ensures better reproducibility
and higher precision.
° Positive Pressure achieved via Gilson’s Integrated
Sealing foot design for both cartridges or plates.
on-bed injectors,
on-bed solvent transfer
ports, on-bed rinse pumps,
and on-board solvent
reservoirs allow you
Gilson’s Integrated Sealing Foot Design
to customize your
SPE 215 mobile racks
System Overview
instrument to meet
your specific needs.
Cartridge Types
1, 3 mL Cartridges, 96-well extraction plate
Air Push Modes
Syringe or Gas Cylinder from Solenoid Valve
On Bed Options
Reservoirs, Online Injection via 849 Multiple Injection Module
Dimensions (w x d x h)
97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in)
Cartridge Capacities*288–1 mL cartridges (tables)
144–3 mL cartridges
3–96 well extraction plates
*Assumes 3 tray positions used for SPE mobile racks.
SPE 215
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Solid Phase Extraction Solutions
Trilution™ LH Software
The complete software solution for your liquid handling and SPE applications
Complete control
for automated
solid phase
extraction (SPE)
applications.
This intuitive software offers
easy-to-use capabilities for the
every day user.
° Easy-to-use, user-friendly graphical
interface minimizes the learning
curve while maximizing efficiency.
Our latest software
package, Trilution™ LH
° Over 70 Gilson-supplied tasks makes
programming methods and creating
applications quick and easy.
° One software package to control all
Liquid Handling and SPE instruments
maximizes the utility of the hardware.
Bed Layout in Method Builder
Add Task
easy to use and most
powerful software yet.
° The Application Run Screen provides
complete control over your run; from set
up to application run, simulation and the
modification of your run.
Designed with the user
in mind, the software is
Derivatize Task
° Drag-and-drop functionality enables
you to quickly and easily set-up
instrument configuration and define
bed layouts.
v.2.0, is Gilson’s most
° Import / Export functionality allows you
to transfer methods from one system to
another quickly and easily.
Method in Method Builder
extremely easy-to-use,
Dispense
Random Task
Custom Task Builder
SPE Condition
Task
SPE Dry Task
SPE Elute Task
SPE Evaporate
Task
SPE Load Task
SPE Wash Task
° Improved sample list editor makes building
your application easier than ever with
spread sheet data entry and functionalities
such as copy down and increment.
° Graphical task pages organize data entry,
allowing the user to quickly create and
modify task properties.
and flexible allowing
° Simulation Mode allows the user to test
and verify methods and applications prior
to running the instrument.
you to focus on the
science rather than
the software.
Tips Dilute
Serially Task
Tips Rinse Task
Simulation in Application Run Screen
SS Dispense Task Property Page
System Overview
Processor
Intel Pentium 4 (2.0 GHZ)
Memory
1.0 GB RAM, 40 GB Hard Drive
Disk
CD Rom
Connections
Two Serial Ports
Resolution
1024 x 768 True Color Video
Operating SystemWIN2K SP3 or WIN XP Professional SP1
Wait Task
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° Dynamic, graphical property pages ensure
quick and easy data entry and variable
creation, while eliminating long variable
lists creation giving you the ultimate
flexibility with minimal effort.
° Manual Control pages allow you
to perform routine tasks,
troubleshoot the instrument, and
set up the instrument prior to
running your applications.
GX-271 Manual Control Page
Solid Phase Extraction Solutions
10
Code 300 Series Racks
Extend the capabilities of your Liquid Handling instrument
SPE Process
Whether looking for high
throughput, pre-injection cleanup
or simple sample prep before
injection users can customize
their Gilson-based systems with
System Overview
Conditioning
Loading Sample
Washing
Eluting
Automating SPE increases throughput
Cartridge Types1, 3, and 6 mL Cartridges.
Instrument
Compatibility
All instruments capable of using Code 200 Series Racks
Rack Code 300
Options Rack Code 300: 1 mL cartridge w/ 96 well deep microplate collection
Rack Code 301: 1 mL cartridge w/ 96 well shallow microplate collection
Rack Code 302: 1 mL cartridge w/ 48 12x32 mm vials (2 mL)
Rack Code 303: 3 mL cartridge w/ 48 12x32 mm vials (2 mL)
Rack Code 304: 3 mL cartridge w/ 48 12x75 mm tubes
Rack Code 305: 3 mL cartridge w/ 32 15x45 mm vials (4 mL)
Rack Code 306: 6 mL cartridge w/ 20 12x75 mm tubes
Rack Code 307: 6 mL cartridge w/ 20 15x45 mm vials (4 mL)
and improves reproducibility, while
minimizing the exposure of the
researcher to harmful chemicals.
Gilson’s instruments are designed to
be workhorse instruments that can be
set up quickly, the application can be
started and the researcher can work
the Code 300 Series mobile racks
allowing the user to combine
Analyte
The Code 300 Series Racks now allow virtually all of Gilson’s existing
on other tasks while the instrument
Interferences
finishes the application effortlessly.
instrumentation to perform SPE, filtration, and liquid handling tasks.
the robustness of Gilson’s liquid
handlers with mobile
This minimizes the cost of labor
Combine all the features and functionality of the Gilson’s Liquid
SPE Samples Processed per Day
handlers and the time tested mobile rack technology to customize
(Assuming a 10 min run time)
SPE / purification needs.
960
° The Code 300 racks accomodate 1, 3, and 6 mL cartridges.
Code 300 Series
° The
Racks can be used on
any Gilson instrument
that accommodates a
Code 200 Series Rack,
effectively transforming
automated liquid handling
instrument into a Solid
Phase Extraction systems.
° Compatible with singleprobe, multiple-probe
fixed and variable-span
instruments.
be performed without a researcher
present. With single, four probe, and
eight probe parallel processing units,
Samples Processed
customized solution for their
typically associated with manual
SPE processes, and allows SPE to
your instrument to your specific application needs.
rack technology to create a
Why automate SPE?
you decide what instrument provides
480
the throughput needed for your
application.
50
Researcher
120
GX-271
ASPEC
GX-274
ASPEC
SPE
215
Manual
Gilson’s Automated SPE Solutions
Requires Researcher
Presence.Assumes the
use of a 10 position
SPE Vacuum block.
Designed to Run Unattended
(20+ hours / day)
(Run Time Limited
to 8 hours / day)
11
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Solid Phase Extraction Solutions
12
Gilson, Inc. World Headquarters
3000 Parmenter Street
Middleton, WI 53562 USA
Telephone: 608-836-1551 or 800-445-7661
Fax: 608-831-4451
www.gilson.com
Gilson S.A.S.
19, avenue des Entrepreneurs
F-95400 VILLIERS LE BEL France
©2006 Gilson, Inc. LT303017-03 Printed in the USA. Specifications subject to change without notice.
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