SPE Solid Phase Extraction Solutions Introduction All of Gilson’s SPE and Liquid Gilson offers the broadest array of SPE instruments on the market today, including the Handling instruments are controlled GX-271 ASPEC , the GX-274 ASPEC , the SPE 215, and virtually any other Gilson instrument via Trilution™ LH software. that accommodates the Code 300 Series racks. Trilution LH has been designed ™ ™ with you, the user, in mind. The Fundamental to Gilson’s automated Solid Phase Extraction (SPE) system is our mobile rack and positive graphical interfaces decrease the pressure technology. The mobile rack technology allows you to automate your entire SPE and Liquid learning curve while increasing Handling processes from the initial condition to the final transfer. Gilson’s SPE instrumentation utilizes your efficiency. Trilution LH allows positive pressure to ensure more reproducible flow rates, more reproducible recoveries, less sample to you to concentrate on the science, sample contamination, and higher precision. not the software. Trilution™ LH provides an intuitive interface for creating SPE and Liquid Handling protocols / As is the case with all Gilson applications to condition cartridges, load the sample, wash the cartridge, elute the analyte of interest and transfer the eluent to a final sample vessel or inject it directly onto an analysis system. Available as a single probe, SPE 215 instruments, the SPE and Liquid Handling instruments are extremely versatile, modular, and robust. Gilson’s SPE instruments are versatile enough to adapt as your science changes and multiple probe, or spannable robust enough to guarantee a low cost of ownership and a workhorse instrument for years to come. multiple probe instrument Gilson has a SPE and Liquid Handling instrument solution to fit your needs. Solid Phase Extraction System Features . . . . . . . . . . 1–2 GX-271 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 3–4 GX-274 ASPEC™ . . . . . . . . . . . . . . . . . . . . . . . . 5–6 SPE 215 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7–8 Trilution™ LH . . . . . . . . . . . . . . . . . . . . . . . . . 9–10 Code 300 Series Racks . . . . . . . . . . . . . . . . . . . . . 11 Automating SPE . . . . . . . . . . . . . . . . . . . . . . . . 12 GX-271 ASPEC i 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions ii Solid Phase Extraction System Features Pressure is constantly monitored and recorded through a pressure sensing module to immediately Optional peripherals such as injectors, identify a change in performance, cartridge failure, on-bed solvent reservoirs, 3-way valves and or plugged solvent lines. peristaltic pumps extend the capabilities of your instrument and allow you to tailor the instrument to meet your specific needs. Complete control via Trilution™ LH, Gilson’s newest and most powerful liquid handling software yet. The 406 Single and Dual Pumps deliver Mobile rack positive pressure via a gas cylinder or to technology allows achieve more accurate flow rates use the the user to perform syringes for the air push. typical Liquid Handling tasks as well as SPE tasks Compatible with the most common and all from the same widely accepted SPE cartridges and bed layout. 96-well extraction plates. The Gilson instruments are capable of utilizing Positive Pressure 1, 3, and 6 mL SPE cartridges as well elutions guarantee as 96-well extraction plates. more reproducible results, higher percent recoveries, On-bed rinse stations minimize and a more carryover and contamination concerns. consistent flow rate. GX-274 ASPEC with two 406 Dual Syringe Pumps 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions GX-271 ASPEC™ 6mL 3mL 1mL Automated Solid Phase Extraction, Liquid Handling and On-line Injection capabilities Whether your application is pharmaceutical, clinical, forensic, or environmental. SPE has become the technique of choice for sample cleanup, sample prep, System Overview Cartridge Types 1, 3, 6 mL Cartridges Air Push Modes Syringe or Gas Cylinder Valve On Bed Options Reservoirs, Direct Injection Module Dimensions (w x d x h) 59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in) in both batch and sequential mode to increase ° Operate throughput and efficiency. n-bed reservoirs allow access up to four solvents ° O without taking up a valuable rack position ° Accommodates 1, 3 and 6 mL SPE cartridges. Cartridge Capacities*108–1 mL cartridges 60–3 mL cartridges 45–6 mL cartridges ° Mobile Rack Technology enables use as both a SPE instrument as well as a Liquid Handling instrument. *Assumes 3 tray positions used for SPE mobile racks. ° Multiple rinse stations with optional rinse pump allows multiple rinsing parameters depending on application need. Designed with today’s laboratory in mind, the GX instruments have large cartridge capacities while minimizing the amount of ° The 406 Single Syringe Pump is equipped with a pressure sensing module that monitors pressure and can be used to handle error conditions such as high cartridge pressure, or clogged liquid lines. linear bench space consumed. System performance is constantly and trace analyte enrichment because of its inherent versatility, selectivity, speed and low solvent usage. monitored by a pressure sensing modules. Carryover and contamination concerns are minimized through the use of Gilson’s GX-271 ASPEC with on bed solvent reservoirs revolutionary Jet Wash rinse stations. Combining the throughput and ability to run unattended makes this instrument an ideal choice for SPE, sample prep, sample clean up and GPC / SPE applications. 406 Syringe Pump On Bed Injection Capabilities 06 Single Syringe Pump ° 4 enables air pushes from both syringes and external air sources. product can be fractionated by moving to ° Eluted different collection tubes for applications such as buffer or pH step gradients. ° GX Direct Injection Module can be integrated for performing on-line injections onto HPLC or LC/MS systems. ° Positive pressure achieved via Gilson’s unique and innovative sealing cap design. ° Modular design allows the use of Code 20, Code 200, and Code 300 series racks. Mobile racks allow access to both SPE cartridges as well as collection vials GX-271 ASPEC 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions GX-274 ASPEC™ 6mL 3mL 1mL Increased throughput through parallel processing System Overview Maximize your efficiency and Cartridge Types 1, 3, 6 mL Cartridges Air Push Modes Syringe or Gas Cylinder Valve On Bed OptionsReservoirs, Four Probe Off-Bed Solvent Transfer Port throughput by unattended, Dimensions (w x d x h) 59.6 x 53.9 x 57 cm (23.5 x 21.25 x 22.46 in) Cartridge Capacities*108–1 mL cartridges 60–3 mL cartridges 45–6 mL cartridges parallel processing *Assumes 3 tray positions used for SPE mobile racks. of samples. Gilson’s multiple-probe instruments ° Four probe Z-arm increases throughput and efficiency. ° On-bed reservoirs allow access up to four solvents without taking up a position on the tray. give you this ability. ° On bed transfer ports allows access to 2 additional large volume solvent reservoirs without taking up any on bed rack positions. Extend this capability through the use of a VALVEMATE® II. 1. Place the samples on the instrument, SPE cartridges in the mobile racks, and fill any solvent reservoirs. VALVEMATE® II Valve Actuator 2. Create your sample list The GX-274 ASPEC is a high throughput four-probe instrument capable 3. Press Start... its that easy. Collection vessel access of processing up to 4 samples in parallel increasing both throughput and efficiency of your cartridge-based applications. Based on positive pressure elutions this instrument processes up to four samples in design allows the use ° Modular of Code 20, Code 200, and Code 300 series racks. 406 Dual Syringe Pumps are equipped with four independents ° The pressure sensing modules that monitor pressure on each of the four fluid paths and can be used to handle error conditions such as high cartridge pressure, or clogged liquid lines. parallel with an innovative sealing cap technology, where each cartridge is sealed, run, and monitored individually. The two 406 Dual Syringe Pumps allow the user to transfer liquids, process up to four samples in parallel, and to monitor each fluid path individually. ° Accommodates 1, 3 and 6 mL SPE cartridges. Process four samples in parallel Rack Technology enables use ° Mobile as both a SPE instrument as well as a Liquid Handling instrument. pressure elution achieved via ° Positive Gilson’s sealing cap technology. ° Positive pressure elution ensures better reproducibility and higher precision. ° Eluted product can be fractionated by moving to different collection tubes. ° SPE process can be performed in either batch and sequential mode to increase throughput and efficiency. GX-274 ASPEC 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions SPE 215 System 3mL 1mL 96 well extraction plates High-Throughput Solid Phase Extraction and Sample Purification What else can you do with your Gilson SPE system? Due to Gilson’s modular The SPE 215 System is an easy-to-use, powerful, sample preparation solution for your laboratory. This instrument uses Gilson’s Integrated Sealing foot design to achieve it’s positive pressure elutions. Gilson’s multiple probe technology provides the high throughput capability and the ability to increase design you have the ability to match your instrument to your exact needs. Peripherals such as and improve your laboratory’s output and results. Dual Rail™ technology provides accurate and even pressure across all eight syringes allowing for the selection of one to eight probes. The integration of these technologies provides you with a solid SPE solution and results in increased productivity and efficiency in your laboratory. ° Mobile Rack Technology enables use as both a SPE instrument as well as a Liquid Handling instrument. ccommodates 1 and 3 mL cartridges or ° A 96-well extraction plates. ° Can be operated in both batch and sequential mode to increase throughput and efficiency. ° Four or Eight probe configurations to increase throughput and efficiency. ° Positive pressure ensures better reproducibility and higher precision. ° Positive Pressure achieved via Gilson’s Integrated Sealing foot design for both cartridges or plates. on-bed injectors, on-bed solvent transfer ports, on-bed rinse pumps, and on-board solvent reservoirs allow you Gilson’s Integrated Sealing Foot Design to customize your SPE 215 mobile racks System Overview instrument to meet your specific needs. Cartridge Types 1, 3 mL Cartridges, 96-well extraction plate Air Push Modes Syringe or Gas Cylinder from Solenoid Valve On Bed Options Reservoirs, Online Injection via 849 Multiple Injection Module Dimensions (w x d x h) 97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in) Cartridge Capacities*288–1 mL cartridges (tables) 144–3 mL cartridges 3–96 well extraction plates *Assumes 3 tray positions used for SPE mobile racks. SPE 215 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions Trilution™ LH Software The complete software solution for your liquid handling and SPE applications Complete control for automated solid phase extraction (SPE) applications. This intuitive software offers easy-to-use capabilities for the every day user. ° Easy-to-use, user-friendly graphical interface minimizes the learning curve while maximizing efficiency. Our latest software package, Trilution™ LH ° Over 70 Gilson-supplied tasks makes programming methods and creating applications quick and easy. ° One software package to control all Liquid Handling and SPE instruments maximizes the utility of the hardware. Bed Layout in Method Builder Add Task easy to use and most powerful software yet. ° The Application Run Screen provides complete control over your run; from set up to application run, simulation and the modification of your run. Designed with the user in mind, the software is Derivatize Task ° Drag-and-drop functionality enables you to quickly and easily set-up instrument configuration and define bed layouts. v.2.0, is Gilson’s most ° Import / Export functionality allows you to transfer methods from one system to another quickly and easily. Method in Method Builder extremely easy-to-use, Dispense Random Task Custom Task Builder SPE Condition Task SPE Dry Task SPE Elute Task SPE Evaporate Task SPE Load Task SPE Wash Task ° Improved sample list editor makes building your application easier than ever with spread sheet data entry and functionalities such as copy down and increment. ° Graphical task pages organize data entry, allowing the user to quickly create and modify task properties. and flexible allowing ° Simulation Mode allows the user to test and verify methods and applications prior to running the instrument. you to focus on the science rather than the software. Tips Dilute Serially Task Tips Rinse Task Simulation in Application Run Screen SS Dispense Task Property Page System Overview Processor Intel Pentium 4 (2.0 GHZ) Memory 1.0 GB RAM, 40 GB Hard Drive Disk CD Rom Connections Two Serial Ports Resolution 1024 x 768 True Color Video Operating SystemWIN2K SP3 or WIN XP Professional SP1 Wait Task 608-836-1551 • 800-445-7661 • www.gilson.com ° Dynamic, graphical property pages ensure quick and easy data entry and variable creation, while eliminating long variable lists creation giving you the ultimate flexibility with minimal effort. ° Manual Control pages allow you to perform routine tasks, troubleshoot the instrument, and set up the instrument prior to running your applications. GX-271 Manual Control Page Solid Phase Extraction Solutions 10 Code 300 Series Racks Extend the capabilities of your Liquid Handling instrument SPE Process Whether looking for high throughput, pre-injection cleanup or simple sample prep before injection users can customize their Gilson-based systems with System Overview Conditioning Loading Sample Washing Eluting Automating SPE increases throughput Cartridge Types1, 3, and 6 mL Cartridges. Instrument Compatibility All instruments capable of using Code 200 Series Racks Rack Code 300 Options Rack Code 300: 1 mL cartridge w/ 96 well deep microplate collection Rack Code 301: 1 mL cartridge w/ 96 well shallow microplate collection Rack Code 302: 1 mL cartridge w/ 48 12x32 mm vials (2 mL) Rack Code 303: 3 mL cartridge w/ 48 12x32 mm vials (2 mL) Rack Code 304: 3 mL cartridge w/ 48 12x75 mm tubes Rack Code 305: 3 mL cartridge w/ 32 15x45 mm vials (4 mL) Rack Code 306: 6 mL cartridge w/ 20 12x75 mm tubes Rack Code 307: 6 mL cartridge w/ 20 15x45 mm vials (4 mL) and improves reproducibility, while minimizing the exposure of the researcher to harmful chemicals. Gilson’s instruments are designed to be workhorse instruments that can be set up quickly, the application can be started and the researcher can work the Code 300 Series mobile racks allowing the user to combine Analyte The Code 300 Series Racks now allow virtually all of Gilson’s existing on other tasks while the instrument Interferences finishes the application effortlessly. instrumentation to perform SPE, filtration, and liquid handling tasks. the robustness of Gilson’s liquid handlers with mobile This minimizes the cost of labor Combine all the features and functionality of the Gilson’s Liquid SPE Samples Processed per Day handlers and the time tested mobile rack technology to customize (Assuming a 10 min run time) SPE / purification needs. 960 ° The Code 300 racks accomodate 1, 3, and 6 mL cartridges. Code 300 Series ° The Racks can be used on any Gilson instrument that accommodates a Code 200 Series Rack, effectively transforming automated liquid handling instrument into a Solid Phase Extraction systems. ° Compatible with singleprobe, multiple-probe fixed and variable-span instruments. be performed without a researcher present. With single, four probe, and eight probe parallel processing units, Samples Processed customized solution for their typically associated with manual SPE processes, and allows SPE to your instrument to your specific application needs. rack technology to create a Why automate SPE? you decide what instrument provides 480 the throughput needed for your application. 50 Researcher 120 GX-271 ASPEC GX-274 ASPEC SPE 215 Manual Gilson’s Automated SPE Solutions Requires Researcher Presence.Assumes the use of a 10 position SPE Vacuum block. Designed to Run Unattended (20+ hours / day) (Run Time Limited to 8 hours / day) 11 608-836-1551 • 800-445-7661 • www.gilson.com Solid Phase Extraction Solutions 12 Gilson, Inc. World Headquarters 3000 Parmenter Street Middleton, WI 53562 USA Telephone: 608-836-1551 or 800-445-7661 Fax: 608-831-4451 www.gilson.com Gilson S.A.S. 19, avenue des Entrepreneurs F-95400 VILLIERS LE BEL France ©2006 Gilson, Inc. LT303017-03 Printed in the USA. Specifications subject to change without notice.
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