SPRIworks Systems Fully automated library construction systems with built-in size selection and cleanup Push button. Walk away. BR-15981B SPRIworks Technology Fully automated fragment library construction systems Choose the Automated Sample Prep System That is Right for Your Lab History of Trust: Sample Prep for Genomic Sequencing Low-Medium Throughput (Up to 30 libraries/day) High Throughput (Up to 96 libraries/day) SPRIworks Fragment Library Systems SPRIworks HT System • Fully automated library construction with built-in size selection and enzymatic cleanup • Fully automated library construction, with built-in size selection and enzymatic cleanup • Process up to 30 samples per day • Process up to 96 samples per day • Bench-top system • SPRIworks chemistry automated on Biomek robotic platforms 2010 - Beckman Coulter utilizes SPRI chemistry for next generation sequencing sample preparation with the launch of SPRIworks Fragment Library Systems. • Less than 5 minutes hands-on setup time 2005 - Recognizing the value of SPRI chemistry optimized on Biomek automated workstations, • Prepares samples for Illumina*, Roche* and Life Technologies* next generation sequencers • Less than 30 minutes hands-on preparation time • Prepares samples for Illumina next generation sequencers Beckman Coulter acquires Agencourt Bioscience. • Includes optional methods for qPCR† setup normalization and pooling 2003 - As a result of the high quality sequencing data produced by Agencourt, the NHGRI designates Agencourt as one of only five Large-Scale Sequencing Centers and the only commercial center responsible for genome sequencing of organisms of high biomedical importance. SPRI technology, robotics and bioinformatics enable Agencourt to be acknowledged by the sequencing community as one of the preeminent sequencing facilities. Beckman Coulter SPRIworks vs Manual Processing Methods Manual Up to 8 libraries per day SPRIworks Up to 30 libraries per day SPRIworks HT Up to 96 libraries per day DNA Extraction DNA Extraction DNA Extraction Shear DNA Shear DNA Shear DNA End Repair End Repair End Repair 2001 - Due to SPRI’s flexibility, ability to produce high quality nucleic acid, and ease of automation, Agencourt launches sequencing services and genomics products based on SPRI chemistry. 1995 - Agencourt recognizes the advantages of magnetic bead technology and acquires the license for SPRI paramagnetic beads. SPRI is immediately used as the platform for all products and services. Clean-up A-Tailing Clean-up Ligation A-Tailing Clean-up Ligation Library construction is the number one bottleneck in the sample preparation process for next generation sequencing, requiring significant hands-on time. Additionally, multiplexing capabilities are putting strain on laboratories to prepare more libraries for sequencing than ever before. SPRIworks and SPRIworks HT Fragment Library Systems eliminate manual library construction and provide a significant boost to productivity, reproducibility and consistency. By automating sample processing, laboratories can now maximize the return on their sequencing investments while freeing up valuable resources to focus on data analysis and biological interpretation. Size Selection Automated 1-30 fully sized libraries per day A-Tailing Clean-up Ligation Clean-up Clean-up Fully Automated Library Construction Clean-up Clean-up Manual 1-8 fully sized libraries per day per person Size Selection Clean-up Automated (optional) Covaris E-series can be integrated on Biomek FXP Automated 1-96 fully sized libraries per day Clean-up Size Selection Clean-up Clean-up PCR Set-up PCR Set-up PCR Set-up PCR PCR PCR PCR Clean-up PCR Clean-up PCR Clean-up qPCR qPCR qPCR Normalization Normalization Normalization Automated (optional) T-robot on Biomek FXP Automated with method suite Automated with method suite Pooling Pooling Pooling Sequencing Sequencing Sequencing SPRIworks Fragment Library Systems I, II and III For Illumina, Roche and Life Technologies next generation sequencers Built-in SPRI Size Selection and Cleanup Chemistry Increase library construction throughput without running gels, cutting bands for DNA fragment size selection or using columns for cleanup. Free Your Lab From the Constraints of Next Generation Sequencing Sample Prep The Beckman Coulter SPRIworks Fragment Library Systems improve laboratory productivity and liberate scientists to spend time focusing on data analysis. Run up to 10 samples at a time • Maximize sequencing run capacity by multiplexing up to 10 samples in parallel on each system No more manual size selection gels, cleanup columns or manual fragment library construction. Fully automated library construction process • Reproducible and consistent library output SPRI-TE Nucleic Acid Extractor • Only 5 minutes of hands-on time Reagents predispensed in a sealed cartridge 200-400 size selection • Simple library construction process 300-600 size selection Short and long fragment sizes isolated using SPRIworks Fragment Library System I for Illumina GA Sequencers and analyzed using Agilent* High Sensitivity DNA Chip. • No more labor intensive pipetting Multi-platform Library Construction Method Card Not only does the SPRIworks bench-top instrument occupy a small footprint, but it prepares fragment libraries for Illumina, Roche and Life Technologies next generation sequencers. Cartridge SPRIworks Fragment Library Systems have three components; The SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. System I for Illumina Sequencers System II for Roche Sequencers System III for Life Technologies Sequencers Throughput Up to 10 samples in about 5 hours Up to 10 samples in under 4 hours Up to 10 samples in under 4 hours DNA input amount 3-5 µg 500 ng 1 µg (3 µg for 150-250 size selection) Size selection ranges • No size selection • 200-400 bps • 300-600 bps • No size selection • 350+ • 500+ • No size selection • 150-250 bps • 150-350 bps Supported input sample types Sheared DNA, cDNA, amplicons Sheared DNA, cDNA, amplicons Sheared DNA, cDNA, amplicons Basic Operation Steps SPRIworks systems are simple and easy to operate. Hands-on time is significantly decreased so that technical expertise can be allocated to experiment design and data analysis. Step 1. Load cartridge into rack Step 2. Transfer input samples Step 3. Load cartridges, samples, adapters and labware Step 4. Insert method card, select size range and press START SPRIworks HT High Throughput System For Illumina next generation sequencers Built In SPRI Size Selection and Cleanup High throughput library construction without running gels, cutting bands or using columns for cleanup. Sample Prep Freedom for High Throughput Next Generation Sequencing Labs Patented SPRI chemistry is combined with the flexibility of the Beckman Coulter Biomek FXP liquid handler to revolutionize how next generation sequencing samples are prepared. All chemistry needed to prepare libraries is included in a kit format along with the reagents to perform size selection and chemistry cleanup. The only thing left to do is add the DNA sample and adapter of choice. Because SPRIworks HT is automated on the Biomek automated liquid handler, users have access to a suite of methods to further streamline the next generation sequencing workflow. Such methods include: PCR setup, PCR (on deck with T-robot integration), PCR cleanup, quantitation, normalization, and sample pooling. Fully automated library construction process With an Intuitive Easy to Use Interface, Everyone is an Expert • Less than 30 minutes of hands-on time High throughput Step 1. Configure parameters using the intuitive user interface • Up to 96 high quality, fully constructed and sized libraries in under 6 hours • Up to 25 times the throughput of manual library construction, similar to having 25 additional people to do next generation sequencing sample preparation Simple graphical user interface 150-350 size selection Short fragment sizes isolated using SPRIworks HT High Throughput System and analyzed using the Agilent High Sensitivity DNA Chip. Illumina paired end adapters were used, samples were purified with Agencourt AMPure XP and amplified with 10 cycles of PCR. Includes SPRI size selection and cleanup High Throughput Library Construction Up to 96 high quality, fully constructed and sized libraries in under 6 hours. SPRIworks HT Throughput Up to 96 samples in under 6 hours DNA input amount 1 µg Size selection options • No size selection • 150-350 bp (insert sizes) • 250-450 bp (insert sizes) • 350-700 bp (insert sizes) Supported input sample types Sheared DNA, gDNA, cDNA, amplicons Step 2. Method calculates reagent volumes based on user input Step 3. Place tips, plates and reagents on the instrument, click OK Ordering Information In the United States and Canada call +1 800 361 7780 In other countries, please contact your local representative or call the country number listed below. SPRIworks Fragment Library Systems (Up to 30 libraries/day) Product # SPRIworks System I (Includes instrument, cartridges, method card and labware) SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 10 cartridges SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 50 cartridges A88267 A84801 A84803 SPRIworks System II (Includes instrument, cartridges, method card and labware) SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 10 cartridges SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 50 cartridges A88268 A84805 A84806 SPRIworks System III (Includes instrument, cartridges, method card and labware) SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 10 cartridges SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 50 cartridges A98473 A98476 A98477 SPRIworks HT System (Up to 96 libraries/day) Product # SPRIworks HT Reagent kit (For Illumina next generation sequencers) 48 samples B06938 Biomek FXP Dual Arm Multi-96 and Span-8 Workstation - SPRIworks HT1 B054502 SPRIworks HT Automation Consumables (Includes Biomek tips, plates, tubes, etc. to process 960 samples) B05451 1 2 Contact your automation sales specialist for specific configurations. +1 800 742 2345 The Biomek Workstation (Product # B05450) is intended for 110V operation only. For more information, please visit www.beckmancoulter.com or contact your local sales representative. For technical support in the United States and Canada call +1 800 854 3633 (Option #, 1, 3, 3). In other countries, please contact your local representative or call the country number listed below. † The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd. * All trademarks are property of their respective owners. Beckman Coulter, the stylized logo, AMPure, Biomek, SPRI and SPRIworks are registered trademarks of Beckman Coulter, Inc. For Research Use Only. Not for use in diagnostic procedures. 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