the New Scie

the New Scie
electrophoresis | radiation safety | laboratory accessories
SCIE-PLAS
product catalogue
Welcome to the latest Scie-Plas product catalogue
This catalogue demonstrates a real depth of product range that covers
all aspects of electrophoresis applications. Our team is constantly
investigating new ways to improve our existing products as well as
looking for new areas of research where our products will be of
relevance and significant benefit.
Scie-Plas Ltd operates in a global market and is recognised worldwide
as a leader and innovator in electrophoresis and associated
techniques. Working closely with our experienced network of dealers
around the globe, Scie-Plas products can be found in most leading
universities, hospitals and research facilities.
In order to satisfy an ever growing global biotech market, Scie-Plas
Ltd recently relocated its manufacturing to a state-of-the-art facility in
San Francisco, CA. Our sales, order entry and accounts team remain
in the UK, but have relocated to a dedicated facility that provides
access to the space that a growing company requires. However, we
will still maintain products in Europe to facilitate rapid order fulfilment
for our customers. We believe that these recent endeavours will
enable Scie-Plas to respond rapidly to our customer enquiries and
ensure that our valued customers receive not only the very best
product, but also the very best customer service.
We would like to thank all of our customers and distribution partners
for their continued support, and we trust that Scie-Plas will remain
your first choice for all of your electrophoresis requirements.
Jeffrey Cohen
Division President
John Attwood
Division Sales & Marketing Director
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This freshly revised product catalogue includes the very latest systems
for electrophoresis applications. Scie-Plas is proud to be specialists
in the field of electrophoresis instrumentation and we believe that our
application focused instruments, our dedicated team of fabrication
specialists, and qualified support team offer our customers a level of
service second to none.
Contents
Contents
Introduction
ii
i
Blotting Units
83-89
The TV100-EBK Mini and TV400-EBK Maxi Electroblotters
The V10-SDB and V20-SDB Semi-Dry Blotters
The CP-2826 Maxi Capillary Blotter
Dot and Slot Blot Microfiltration Manifolds
84
86
87
88
Power Supplies
90-92
Power Supply Accessories
Consort EV200 Range Power Supply Units
90
91
Electrodialysis
93-95
The ElectroPrep™
93
Horizontal Gel Units
1-34
HB-SET Mini-gel
Easigel H1-SET
HU6 Mini Horizontal
HU10 Mini-Plus Horizontal
HU13 Midi Horizontal
HU15 Standard Horizontal
HU20 Maxi-Standard Horizontal
HU25 Maxi-Plus Standard Horizontal
CHU20 Maxi-Cooled Horizontal
CHU25 Maxi-Plus Cooled Horizontal
SVG-SYS Vari-gel Mini System
TVG-SYS Vari-gel Midi System
VG-SYS Vari-gel Maxi System
VG-FAST96 High Throughput Vari-gel Maxi System
Horizontal Gel Casting Products
Scie-Plas Super-Seals™
Accessories & Buffer Recirculation Pump
4
5
6
8
10
12
14
16
18
20
24
26
28
30
32
33
34
Transilluminators
100-101
Specialist Gel Units
36-40
Biostep Transilluminators
100
CA-SYS Cellulose Acetate System
COMET Single Cell Gel Electrophoresis Systems
The IEF-SYS Dedicated Isoelectric Focusing Unit
36
38
40
Radiation Protection
104-115
Shielding
Storage Boxes
Waste Bins
Boxes and Pipette Shields
Transport and Container Covers
Cabinet Workstation
Safety Bags
Safety Trays and Liners
106
109
111
112
113
114
114
115
Vertical Gel Units
43-70
The TV50 Single-Plate Mini-Gel Electrophoresis Unit
TV100 Twin-Plate Mini-Gel Electrophoresis Units
TV200 Twin-Plate Wide Format Mini-Gel
Electrophoresis Units
TV400 Twin-Plate Maxi-Gel Electrophoresis Units
TV Complete Modular Systems
TV Modular Electroblotting Systems
TV Modular 2-D Systems
The TV100YK and TV400YK-2D-IEF-SYS Units:
Dedicated 2-D Electrophoresis Systems
The TVS1400 Single-Plate
Large Format Vertical Gel Electrophoresis Unit
36
38
52
56
60
62
64
67
Gel Documentation
96-97
Scie-Plas VISION Gel Documentation System
GeneTools Software
96
97
PCR® Products
98-99
The GLE-UVSC UV Sterilisation Cabinet
Techne® TC-3000 Thermal Cycler
98
99
Laboratory Equipment
116
Workstation Accessories
Racks
116
116
Reagents
117-125
117
118
126
Gel Drying Systems
73-75
The GD-4534 and GD-5040 Gel Dryers
The Vacuubrand® MZ 2C + 2 AK Vacuum Pump
73
75
Agarose Gel Reagents and Buffers
Polyacrylamide Gel Reagents and Buffers
Isoelectric Focusing: Ampholytes,
Precast Gels and IPG Strips
Precast PAGE Gels
Blotting Buffers & Membranes
Gradient Gel Electrophoresis
76-80
The TV400-DGGE Denaturing Gradient
Gel Electrophoresis System
Gradient Mixers
TV100, TV200 and TV400 Gel Multicasters
Terms and Conditions
76
79
80
Gel Temperature Control
82
Recirculating Cooler/Chiller
82
70
121
123
124
• Robust acrylic tank construction 12mm
walls, leak proof
Scie-Plas horizontal gel electrophoresis units offer the ultimate
standards in innovative design and manufacture, acquired
during our 20+ years existence.
Each model incorporates the many design and safety features
recommended to us by scientists, either from within our inhouse product development team or laboratories worldwide. As
a result, all our models are manufactured and finished to the
highest specification, easy to use, and, with safety always
paramount, CE-marked - conforming to the latest European
safety regulations.
• Safety lid with integral power leads
• Shrouded 4mm power output connectors
universally compatible with modern-day
low to medium voltage power supplies
• Long-life, 99.99 % pure platinum
electrodes
• Gold-plated terminals with corrosionfree stainless steel washers
• Durable high-impact polystyrene (HIPS)
combs
• UV-transparent acrylic gel-casting trays
• Offset asymmetric electrodes
• Colour-coded combs corresponding to 1,
1.5 and 2mm thicknesses
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Horizontal Gel Units
BENEFITS INCLUDE
Power Connections offset and asymmetric for
correct polar orientation
Horizontal Gel Units
Lift-Off Lid fits on one way only,
preventing access to
live components.
Disconnects tank
immediately from
power supply once
lid is removed
4mm Power Output
Connectors compatible with
modern low to
medium voltage
power supplies
Leak-Free Tank manufactured
from robust 12mm
green acrylic side
panels bonded to
5mm clear acrylic
bended sheet
Handles for safe and easy
transportation
around the
laboratory
Cooling Ports connect cooled base
to water supply or
external chiller on
CHU20 and CHU25
units only
Gel Casting Tray UV transparent
with comb slots
and end-grooves
for seating casting
gates
White Gel Table for easier
visualisation of
marker dyes and
samples as they
migrate through
the gel
Combs - heightadjustable in Green
Range units and
colour-coded for
thickness
Buffer Recirculation Ports
- can be connected to a
peristaltic pump, available
on HU13, HU15, CHU20,
HU20, CHU25 and HU25
units only
0.2mm
Platinum
Electrodes 99.9% purity
and userreplaceable
Coloured strips enhance sample
visibility during
loading
Gel Casting Options - available in multiple internal and external formats
1. Plastic casting gates
2. Silicone gaskets for
direct in-tank casting
3. Silicone casting gates
5. External casting unit for
4. Casting gates with
slot into preformed grooves integral silicone seals
a maximum of 3 gels
6. Super-Seals for
flexible gel casting
CASTING OPTION AVAILABLE WITH THE GREEN RANGE HORIZONTALS
CASTING OPTION
1. Plastic casting gates
2. Silicone gaskets
3. Silicone casting gates
HB-SET
H1-SET
HU6
HU10
HU13
4. Casting gates with
integral silicone seals
5. External casting unit
6. Super-Seals™
2
HU15
HU20
CHU20
HU25
CHU25
The Scie-Plas horozontal range entails five individual series determined by gel tray size and sample throughput: The MINI and MINI-PLUS series, which comprise the HB-SET, H1-SET, HU6 and HU10 units, are ideal for screening and analysis of a
wide range of samples, including PCR products, DNA mini-preps, plasmid vectors and restriction fragments. Each unit’s compact size
optimises the gel conditions and voltage gradient, allowing fast resolution of nucleic acids.
APPLICATIONS
COMPLETE SYSTEM
Teaching
Routine preparatory /
analytical gel electrophoresis
Low throughput nucleic
acid screening
Part No.
MINI
Battery-powered horizontal mini-gel unit with built-in 6 x 7.5cm (W x L) casting tray
‘Easigel’ fast-mini horizontal gel unit with built-in 10 x 8cm (W x L) casting tray
Mini horizontal gel unit with removable 6 x 7.5cm (W x L) casting tray
HB-SET
H1-SET
HU6
MINI-PLUS
HU10
The MIDI series, which includes the HU13 unit, accommodates standard gels for medium throughput analysis and preparation of
nucleic acids, while the STANDARD HU15 provides the same comb-throughput and tray size options as industry standard units, with
additional casting options.
APPLICATIONS
COMPLETE SYSTEM
Preparatory gel
electrophoresis
Analytical gel
electrophoresis
Medium throughput
nucleic acid screening
Part No.
HU13
HU15
MIDI
Midi horizontal gel unit with removable 12.8 x 15cm (W x L) casting tray
STANDARD
Standard Format Midi horizontal gel unit with removable 15 x 15cm (W x L) casting tray
The MAXI and MAXI-PLUS series, available in standard (HU20 and HU25) and cooled (CHU20 and CHU25) formats, are designed for the
rapid, medium to high throughput screening of nucleic acids, such as HLA typing in population genetics studies. The cooled bases
within the CHU20 and CHU25 units make these units ideal for hospital, university and industrial laboratories, which need to perform
fast, large-scale analysis of nucleic acids at high voltage, but without compromising resolution.
APPLICATIONS
COMPLETE SYSTEM
Medium throughput
nucleic acid screening
MAXI
Maxi-Standard horizontal gel unit with removable 20 x 20cm (W x L) casting tray
Maxi-Cooled horizontal gel unit with removable 20 x 20cm (W x L) casting tray
MAXI-PLUS
Maxi-Plus Standard horizontal gel unit with removable 25 x 30cm (W x L) casting tray
Maxi-Plus Cooled horizontal gel unit with removable 25 x 30cm (W x L) casting tray
High throughput
nucleic acid screening
Part No.
HU20
CHU20
HU25
CHU25
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Mini-Plus horizontal gel unit with removable 10 x 11.5cm (W x L) casting tray
Horizontal Gel Units
BENEFITS INCLUDE
• Low voltage - 2 non-rechargeable PP3
batteries, providing a maximum 18-Volt
output, make the HB-SET ideal for use
in schools and colleges where safety is
paramount
• Integrated safety lid - ensures that the
gel remains inaccessible during use
• UV-transparent acrylic - for direct
visualisation of the gel within the tank,
limiting the user’s exposure to
hazardous ethidium bromide
• Ultra-compact tank - minimises the
buffer volume required to cover the gel,
providing optimal control over the
voltage gradient and run-time
• Small size - allows safe and easy
transportation from the bench to the
transilluminator
• Cost-effective - no need for expensive
power supplies, while built-in plastic
casting gates allow direct gel casting in
the tank without additional costly
accessories
HB-SET Mini-gel
The HB-SET battery mini-gel electrophoresis unit is designed
specifically with safety and cost in mind: in that no power supply
is necessary.
TECHNICAL SPECIFICATION
Unit Dimensions (W x L x H)
TECHNICAL | Tip:
Enhance Resolution: 2 x TAE buffer can be used in units
with low buffer volume to enhance resolution during
extended runs.
11 x 17 x 3.5cm
Gel Dimensions (W x L)
6 x 7.5cm
Minimum Buffer Volume
50ml
Maximum Sample Capacity
32
Combs
2
Comb Thickness
1mm
Comb Throughput
8 to 12 samples
Comb Slots
2
Migration Distance
Between Comb Slots
3.5cm
Running Voltage
Recommended Power
Source
ORDERING INFORMATION
Complete System
Horizontal mini-gel unit with built-in casting tray and 2 x 1mm thick,
8-sample combs and 2 x non-rechargeable PP3 batteries
HB-SET
18V
Replacement Parts & Accessories
2 x casting gates
HB-CG
2 x (9V)
PP3 batteries
HB-SET Combs
Part No.
DO YOU NEED…?
AGAROSE
A POWER SUPPLY
A GEL DOCUMENTATION SYSTEM
GEL ANALYSIS SOFTWARE
A UV STERILISATION CABINET
4
SEE PAGE 118
SEE PAGES 91-92
SEE PAGE 96
SEE PAGE 97
SEE PAGE 98
H2-C1-8
H2-C1-12
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
8
12
5.25
3.5
1
1
23
15
BENEFITS INCLUDE
• Plastic casting gates - for direct gel
casting in the tank with no need for
costly accessories
• Colour-coded combs - 1 and 1.5mm
thick, offer flexibility in sample-loading
volume to a maximum 40-sample
throughput
• Quick-release lid - disconnects the tank
and lid simultaneously from the power
supply for safe gel loading and disposal
• Compact design - minimises the buffer
volume required to cover the gel,
providing optimal control over the voltage
gradient and run-time
• Small size - allows safe and easy
transportation from the bench to the
transilluminator
Easigel H1-SET
The Easigel H1-SET offers a swift and simple horizontal gel
electrophoresis solution for standard preparative and analytical
studies of nucleic acids.
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Complete System
‘Easigel’ horizontal gel unit with built-in casting tray and
2 x 1.5mm thick, 8-sample combs
Part No.
H1-SET
Replacement Parts & Accessories
2 x casting gates
2 x 1 metre power leads with shrouded
4mm power output connectors
2 x 0.2mm thick, platinum electrode wire
H1-CG
CABLE-4
PT-0.2
H1-SET Combs
Part No.
H1-C1-8
H1-C1-12
H1-C1-16
*H1-C1-20MC
H1-C1.5-8
H1-C1.5-12
H1-C1.5-16
*H1-C1.5-20MC
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1.5
1.5
1.5
1.5
8
12
16
20
8
12
16
20
10
6
4
2.8
10
6
4
2.8
2
2
2
2
2
2
2
2
45
27
18
12
65
40
27
18
Unit Dimensions (W x L x H)
13.5 x 15 x 3cm
Gel Dimensions (W x L)
10 x 8cm
Minimum Buffer Volume
50 ml
Maximum Sample Capacity
40
Combs
2
Comb Thickness
1 or 1.5mm
Comb Throughput
8 to 20 samples
Comb Slots
2
Migration Distance Between 3cm
Comb Slots
Recommended Running
Voltage (power supply)
70 to 90V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV222
*Multichannel pipette compatible
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• UV-transparent acrylic - for direct
visualisation of the gel within the tank,
limiting the user’s exposure to hazardous
ethidium bromide
Horizontal Gel Units
BENEFITS INCLUDE
• Four casting options - provide total
flexibility in gel casting, either directly
within the tank or externally when the
tank is in use
HU6 Mini Horizontal
The HU6 mini horizontal gel electrophoresis unit is ideal for
routine preparatory and analytical electrophoresis techniques.
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth to a
maximum 32-sample throughput
• Coloured loading strips - for easy well
detection when loading
• Short 6cm gel length - ideal for rapid
separations
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
6
HU6 IN-TANK CASTING OPTIONS
HU6-UT - silicone gaskets, lining the
HU6 casting tray, form a leak proof seal
against the inner walls of the running
chamber when the casting tray is turned
at 90° to the direction of electrophoresis
HU6-SS - 6cm long Scie-Plas Super-Seals
offer total versatility in casting, allowing
the gel length to be tailored to each user’s
personal requirements
Replacement parts and accessories
TECHNICAL SPECIFICATION
ORDERING INFORMATION
Part No.
HU6
Replacement Parts & Accessories
1 x gel casting tray 6 x 7.5cm
2 x silicone casting gates
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
6 x coloured loading strips
1 x external casting unit for 3 gel casting trays
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power
output connectors
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
1
1
1
1.5
1.5
1.5
2
2
2
8
12
16
8
12
16
8
12
16
4.5
2.5
2.2
4.5
2.5
2.2
4.5
2.5
2.2
HU6-C1-8
*HU6-C1-12MC
HU6-C1-16
HU6-C1.5-8
*HU6-C1.5-12MC
HU6-C1.5-16
HU6-C2-8
*HU6-C2-12MC
HU6-C2-16
6 x 7.5cm
Buffer Volume
325ml
32
HU6-UT
HU6-SCG
HU-SG
HU6-SS
HU6-CS
HU6-CU
HU6-GS
PT-0.2
Combs
2
CABLE-4
Max.
Sample Volume
Spacing (mm)
in a 5mm
Deep Well (µl)
2
2
1
2
2
1
2
2
1
Gel Dimensions (W x L)
Maximum Sample Capacity
HU6 Combs
Part No.
Unit Dimensions (W x L x H) 13 x 24 x 6.5cm
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
8 to 16 samples
Comb Slots
2
Migration Distance
Between Comb Slots
3.5cm
Recommended
Running Voltage
70 to 90V
Power Output
Connectors (diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV222
20
11
10
30
17
15
40
22
20
*Multi-channel pipette compatible
HU6 EXTERNAL CASTING OPTIONS
HU6-SCG - silicone casting gates slot into
the grooves at each end of the casting tray
to form a leak-free seal
HU6-CU - silicone gaskets, seated in the
groove of each casting tray, form a leakfree seal against the walls of the casting
unit, allowing 3 gels to be cast
simultaneously
CASTING TIP Regardless of the
casting method, ensure leak-free
casting by lining the casting unit with agarose
and allowing it to set before pouring the
remainder of the hand-hot agarose mixture i.e.
at approx. 50°C
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Complete System
Mini horizontal gel unit with removable casting tray and
2 x 1mm thick, 8-sample combs and coloured loading strips
Horizontal Gel Units
BENEFITS INCLUDE
• Four casting options - provide total
flexibility in gel casting, either directly
within the tank or externally when the
tank is in use
HU10 Mini-Plus Horizontal
The HU10 mini-plus horizontal gel electrophoresis unit is ideal
for routine preparatory and analytical electrophoresis techniques.
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth to a
maximum 80-sample throughput
• Four comb positions - at 2.5cm intervals
along the tray for swift separation of
multiple samples
• Coloured loading strips - for easy well
detection when loading
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
8
IN-TANK CASTING OPTIONS
HU10-UT - silicone gaskets, lining the
HU10 casting tray, form a leak proof seal
against the inner walls of the running
chamber when the casting tray is turned
at 90° to the direction of electrophoresis
HU10-SS - 10cm long Scie-Plas SuperSeals offer total versatility in casting,
allowing the gel length to be tailored to
each user’s personal requirements
Replacement parts and accessories
ORDERING INFORMATION
Complete System
Mini-plus horizontal gel unit with removable casting tray and
2 x 1mm thick, 16-sample combs and coloured loading strips
Part No.
HU10
Replacement Parts & Accessories
1 x gel casting tray 10 x 11.5cm
2 x silicone casting gates
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x external casting unit for 3 gel casting trays
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power
output connectors
HU10-C1-8
*HU10-C1-10MC
HU10-C1-12
HU10-C1-16
*HU10-C1-20MC
HU10-C1.5-8
*HU10-C1.5-10MC
HU10-C1.5-12
HU10-C1.5-16
*HU10-C1.5-20MC
HU10-C2-8
*HU10-C2-10MC
HU10-C2-12
HU10-C2-16
*HU10-C2-20MC
16.5 x 23 x 6.5cm
Gel Dimensions (W x L)
10 x 11.5cm
Buffer Volume
450ml
Maximum Sample Capacity
80
HU10-UT
HU10-SCG
HU-SG
HU10-SS
HU10-CS
HU10-CU
HU10-GS
PT-0.2
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
8 to 20 samples
CABLE-4
HU10 Combs
Part No.
Unit Dimensions (W x L x H)
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
2
2
2
2
2
8
10
12
16
20
8
10
12
16
20
8
10
12
16
20
9
7
5.5
3.6
3
9
7
5.5
3.6
3
9
7
5.5
3.6
3
2.5
2
2
2
1.5
2.5
2
2
2
1.5
2.5
2
2
2
1.5
40
30
25
15
12
60
45
35
25
20
80
60
50
30
25
*Multi-channel pipette compatible
Comb Slots
4
Migration Distance Between
Comb Slots
2.5cm
Recommended Running
Voltage
75 to 125V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV222
DO YOU NEED…?
AGAROSE
SUPER-SEALS™
A GEL LEVELING TABLE
UV TRANSILLUMINATORS
HU10 EXTERNAL CASTING OPTIONS
HU10-SCG - silicone casting gates slot
into the grooves at each end of the
casting tray to form a leak-free seal
HU10-CU - silicone gaskets, seated in the
groove of each casting tray, form a leakfree seal against the walls of the casting
unit, allowing 3 gels to be cast
simultaneously
SEE PAGE 118
SEE PAGE 33
SEE PAGE 34
SEE PAGE 100
TECHNICAL TIP | Gasket too
tight or loose? Simply remove the
gaskets from the ends of the gel
trays and refit into the groove. Refit with the
gasket protruding slightly from the ends if the
gel tray was too tight or with the gasket fitted
below the top edges if the gasket was too
loose.
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TECHNICAL SPECIFICATION
Horizontal Gel Units
BENEFITS INCLUDE
• Four casting options - provide total
flexibility in gel casting, either directly
within the tank or externally when the
tank is in use
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth to a
maximum 112-sample throughput
• Four comb positions - at 3.5cm intervals
along the tray for faster separation of
multiple samples
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to maintain buffer pH
and prevent ionic gradient
formation
• Coloured loading strips - for
easy well detection when loading
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
10
HU13 Midi Horizontal
The HU13 midi horizontal gel electrophoresis unit is ideal for
analytical and preparative studies of nucleic acids.
HU13 IN-TANK CASTING OPTIONS
HU13-UT - silicone gaskets, lining the
HU10 casting tray, form a leak proof seal
against the inner walls of the running
chamber when the casting tray is turned
at 90° to the direction of electrophoresis
HU13-SS - 13cm long Scie-Plas SuperSeals offer total versatility in casting,
allowing the gel length to be tailored to
each user’s personal requirements
TECHNICAL SPECIFICATION
ORDERING INFORMATION
Part No.
Unit Dimensions (W x L x H)
HU13
Replacement Parts & Accessories
1 x gel casting tray 12.8 x 15cm
2 x buffer recirculation ports
2 x silicone casting gates
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x external casting unit for 3 gel casting trays
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1metre power leads with shrouded
4mm power output connectors
HU13-UT
HU-BRP
HU13-SCG
HU-SG
HU13-SS
HU13-CS
HU13-CU
HU13-GS
PT-0.2
CABLE-4
HU13 Combs
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
1.5
2
2
2
2
2
2
10
12
16
20
24
28
10
12
16
20
24
28
10
12
16
20
24
28
9.5
8
5.5
4
3
3
9.5
8
5.5
4
3
3
9.5
8
5.5
4
3
3
2.5
2
2
2
2
1.5
2.5
2
2
2
2
1.5
2.5
2
2
2
2
1.5
40
35
25
17
13
13
60
50
35
25
20
20
85
70
50
35
25
25
HU13-C1-10
HU13-C1-12MC
HU13-C1-16
HU13-C1-20
HU13-C1-24
*HU13-C1-28MC
HU13-C1.5-10
HU13-C1.5-12MC
HU13-C1.5-16
HU13-C1.5-20
HU13-C1.5-24
*HU13-C1.5-28MC
HU13-C2-10
HU13-C2-12MC
HU13-C2-16
HU13-C2-20
HU13-C2-24
*HU13-C2-28MC
20 x 32 x 7cm
Gel Dimensions (W x L)
12.8 x 15cm
Buffer Volume
900ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
112
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
10 to 28 samples
Comb Slots
4
Migration Distance Between
Comb Slots
3.5cm
Recommended Running
Voltage
100 to 125V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
DO YOU NEED…?
ELECTROPHORESIS BUFFERS
COLOURED LOADING STRIPS
A POWER SUPPLY
AN EXTERNAL CASTING UNIT
A GEL SCOOP
SEE PAGE 119
SEE PAGE 34
SEE PAGES 91-92
SEE PAGE 32
SEE PAGE 34
*Multi-channel pipette compatible
HU13 EXTERNAL CASTING OPTIONS
HU13-SCG - silicone casting gates slot
into the grooves at each end of the casting
tray to form a leak-free seal
TECHNICAL TIP | DNA Mobility:
HU13-CU - silicone gaskets, seated in the
groove of each casting tray form a leakfree seal against the walls of the casting
unit, allowing 3 gels to be cast
simultaneously
DNA fragments as small as 1kb or
less can be separated using agarose gel
electrophoresis. For fragments smaller than
0.1kb, polyacrylamide gels are more suitable.
11
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Complete System
Midi horizontal gel unit with removable casting tray and
2 x 1mm thick, 16-sample combs, coloured loading strips and
buffer recirculation ports
Horizontal Gel Units
BENEFITS INCLUDE
• The market standard - a 15 x 15cm gel
tray, with comb options ranging from 1 to
30 samples, is currently the most
popular format in today’s market
• Four comb slots - at 3.5cm intervals
along the tray for faster separation of a
maximum 120 samples
• Multi-channel pipette
compatible combs with a maximum 30sample throughput
reduce gel-loading
time, while
preparatory combs
enable nucleic acids to
be scaled-up for cloning
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth
HU15 Standard Horizontal
The HU15 standard horizontal gel electrophoresis unit provides
the comb throughput and tray-size options most popular in
today’s market, but with additional casting options.
HU15 CASTING OPTIONS
HU15-CG - casting gates with integral
silicone seals effectively seal the tray
without the need for tape, provided the
silicone gasket faces outwards as
shown
• Buffer recirculation ports - may be
connected to a peristaltic
pump for buffer
recirculation during
electrophoresis to
maintain buffer pH and prevent
ionic gradient formation
• Coloured loading strips - for easy well
detection when loading
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
12
CASTING TIP Regardless of
the casting method, ensure
leak-free casting by lining the
casting unit with agarose and allowing it to
set before pouring the remainder of the
hand-hot agarose mixture i.e. at approx.
50°C
HU15-SS - 15cm long Scie-Plas SuperSeals offer total versatility in casting,
allowing the gel length to be tailored to
each user’s personal requirements
Replacement parts and accessories
Complete System
Standard horizontal gel unit with removable casting tray,
including casting gates with integral silicone seals,
2 x 1mm thick, 16-sample combs, coloured loading strips
and buffer recirculation ports
Part No.
HU15
Replacement Parts & Accessories
1 x gel casting tray, 15 x 15cm, including casting gates
with integral silicone seals
2 x buffer recirculation ports
2 x casting gates with integral silicone seals
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Unit Dimensions (W x L x H)
21.5 x 33.5 x 7cm
Gel Size (W x L)
15 x 15cm
Buffer Volume
1200ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
120
Combs
2
Comb Thickness
1, 1.5 or 2mm
HU15-UT
HU-BRP
HU15-CG
HU-SG
HU15-SS
HU15-CS
HU15-GS
PT-0.2
Comb Throughput
1 to 30 samples
Comb Slots
4
Migration Distance
Between Comb Slots
3.5cm
Recommended
Running Voltage
100 to 125V
Power Output
Connectors (diameter)
Shrouded, 4mm
CABLE-4
Recommended
Power Supply
Consort EV243
HU15 Combs
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
1.5
2
2
2
2
2
2
10
15
16
20
25
30
10
15
16
20
25
30
10
15
16
20
25
30
12.8
7
6.4
5.9
3.5
2.5
12.8
7
6.4
5.9
3.5
2.5
12.8
7
6.4
5.9
3.5
2.5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
64
35
32
29
17
12
96
52
48
43
25
18
128
70
64
58
34
24
HU15-C1-10
HU15-C1-15
*HU15-C1-16MC
HU15-C1-20
HU15-C1-25
*HU15-C1-30MC
HU15-C1.5-10
HU15-C1.5-15
*HU15-C1.5-16MC
HU15-C1.5-20
HU15-C1.5-25
*HU15-C1.5-30MC
HU15-C2-10
HU15-C2-15
*HU15-C2-16MC
HU15-C2-20
HU15-C2-25
*HU15-C2-30MC
*Multi-channel pipette compatible
ELECTROPHORESIS
CHEMICALS
SEE PAGES 117-123
13
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TECHNICAL SPECIFICATION
ORDERING INFORMATION
Horizontal Gel Units
BENEFITS INCLUDE
• Large format - 20 x 20cm gel tray is
ideal for high-resolution techniques,
such as screening PCR® products and
RFLP analysis
• Four comb slots - at 5cm intervals along
the tray for faster separation of a
maximum 168 samples
• Multi-channel pipette
compatible combs with a maximum 42sample throughput reduce gel-loading
time
HU20 Maxi-Standard Horizontal
The HU20 maxi-standard horizontal gel electrophoresis unit is
ideal for high-resolution analytical and preparative studies of
nucleic acids.
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to
maintain buffer pH
and prevent ionic
gradient formation
• Coloured loading strips - for easy well
detection when loading
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
• Cooled version available - see page 18
14
HU20 CASTING OPTIONS
HU20-CG - casting gates with integral
silicone seals effectively seal the tray
without the need for tape, provided the
silicone gasket faces outwards as
shown
HU20-SS - 20cm long Scie-Plas
Super-Seals offer total versatility in
casting, allowing the gel length to be
tailored to each user’s personal
requirements
TECHNICAL TIP | RNA
Mobility: Either before or during
electrophoresis, RNA should be
denatured. For example:a. RNA fragments which have been
denatured with glyoxal and dimethyl
sulphoxide can be separated on neutral
agarose gels; or
b. RNA can be fractionated on agarose
gels containing methylmercuric
hydroxide or formaldehyde.
c. RNA samples usually require longer
runs or buffers that are easily depleted,
so it is necessary to circulate the buffer.
Northern analyses should not be run on
a mini-gel tank.
Replacement parts and accessories
Complete System
Maxi-standard horizontal gel unit with removable casting tray,
including casting gates with integral silicone seals, 2 x 1mm thick,
16-sample combs, coloured loading strips and
buffer recirculation ports
Replacement Parts & Accessories
1 x gel casting tray, 20 x 20cm, including casting gates
with integral silicone seals
2 x buffer recirculation ports
2 x casting gates with integral silicone seals
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
HU20
HU20-UT
HU-BRP
HU20-CG
HU-SG
HU20-SS
HU20-CS
HU20-GS
PT-0.2
CABLE-4
HU20 Combs
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1.5
1.5
1.5
1.5
2
2
2
2
16
20
28
40
16
20
28
40
16
20
28
40
8.5
7
5
3
8.5
7
5
3
8.5
7
5
3
3
2
2
2
3
2
2
2
3
2
2
2
35
30
20
13
55
45
30
19
75
60
40
25
HU20-C1-16
*HU20-C1-20MC
HU20-C1-28
*HU20-C1-40MC
HU20-C1.5-16
*HU20-C1.5-20MC
HU20-C1.5-28
*HU20-C1.5-40MC
HU20-C2-16
*HU20-C2-20MC
HU20-C2-28
*HU20-C2-40MC
TECHNICAL SPECIFICATION
Unit Dimensions (W x L x H)
27 x 47.5 x 8cm
Gel Size (W x L)
20 x 20cm
Buffer Volume
2200ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
168
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
16 to 42 samples
Comb Slots
4
Migration Distance Between
Comb Slots
5cm
Recommended Running
Voltage
150 to 175V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
*Multi-channel pipette compatible
15
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ORDERING INFORMATION
Horizontal Gel Units
BENEFITS INCLUDE
• Large format - 25 x 30cm gel tray is
ideal for high-throughput techniques,
such as HLA typing and screening PCR®
products
HU25 Maxi-Plus
Standard Horizontal
• Twelve comb slots - at 2cm intervals
along the tray for rapid, high throughput
separation of a maximum 624 samples
The HU25 maxi-plus standard horizontal gel electrophoresis
unit is ideal for high throughput analysis of nucleic acids.
• Multi-channel pipette compatible
combs - with a maximum 52-sample
throughput - allow samples to be loaded
quickly and easily from 96 and 384-well
thermal cyclers
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to maintain buffer
pH and prevent ionic
gradient formation
• Coloured loading strips - for
easy well detection when loading
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
• Cooled version available - see page 20
16
HU25 CASTING OPTIONS
HU25-CG - casting gates with integral
silicone seals effectively seal the tray
without the need for tape, provided the
silicone gasket faces outwards as
shown
HU25-SS - 25cm long Scie-Plas
Super-Seals offer total versatility in
casting, allowing the gel length to be
tailored to each user’s personal
requirements
TECHNICAL TIP |
Electrophoresis Buffer Selection:
TAE buffer provides optimal
resolution of fragments >4kb in length, while
for 0.1 to 3kb fragments TBE buffer should be
selected. TBE has both a higher buffering
capacity and lower conductivity than TAE and
therefore should be used for high-voltage
electrophoresis. Additionally, TBE buffer
generates less heat than TAE at an equivalent
voltage and does not allow a significant pH
drift.
Replacement parts and accessories
ORDERING INFORMATION
Replacement Parts & Accessories
1 x gel casting tray, 25 x 30cm, including casting gates
with integral silicone seals
2 x buffer recirculation ports
2 x casting gates with integral silicone seals
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
36 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
TECHNICAL SPECIFICATION
HU25
Unit Dimensions (W x L x H)
Gel Size (W x L)
25 x 30cm
Buffer Volume
3000ml
HU25-UT
HU-BRP
HU25-CG
HU-SG
HU25-SS
HU25-CS
HU25-GS
PT-0.2
Buffer Recirculation Ports
2
CABLE-4
HU25 Combs
Part No.
*HU25-C1-26MC
*HU25-C1-52MC
*HU25-C1.5-26MC
*HU25-C1.5-52MC
*HU25-C2-26MC
*HU25-C2-52MC
33 x 56 x 9cm
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1.5
1.5
2
2
26
52
26
52
26
52
7
3
7
3
7
3
2
1.5
2
1.5
2
1.5
30
13
45
20
60
25
Maximum Sample Capacity
624
Combs
6
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
26 to 52 samples
Comb Slots
12
Migration Distance Between
Comb Slots
2cm
Recommended Running
Voltage
150 to 200V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
*Multi-channel pipette compatible
DO YOU NEED…?
SUPER-SEALS™
A POWER SUPPLY
A GEL SCOOP
UV TRANSILLUMINATORS
SEE PAGE 33
SEE PAGES 91-92
SEE PAGE 34
SEE PAGE 100
17
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Complete System
Maxi-plus standard horizontal gel unit with removable casting tray,
including casting gates with integral silicone seals, 6 x 1mm thick,
26-sample combs, coloured loading strips and
buffer recirculation ports
Horizontal Gel Units
BENEFITS INCLUDE
• Cooled base - covering the entire 20 x
20cm gel tray - allows separations to be
performed faster and at higher voltage,
without loss of resolution
• Cooling ports - connect
the cooled base either
to the laboratory water
supply or the optional
external chiller (Page
82) for enhanced
cooling
• Four comb slots - at 5cm intervals along
the tray for faster separation of a
maximum 168 samples
• Multichannel pipette compatible combs
- with a maximum 42-sample throughput
- reduce gel-loading time
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to maintain buffer pH
and prevent ionic gradient formation
• Coloured loading strips - for easy well
detection when loading
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
18
CHU20 Maxi-Cooled Horizontal
The CHU20 maxi-cooled horizontal gel electrophoresis unit
allows analytical and preparative studies of nucleic acids to be
performed quickly at high voltage without compromising
resolution.
CHU20 CASTING OPTIONS
HU20-CG - casting gates with integral
silicone seals effectively seal the tray
without the need for tape, provided the
silicone gasket faces outwards as
shown
HU20-SS - 20cm long Scie-Plas
Super-Seals offer total versatility in
casting, allowing the gel length to be
tailored to each user’s personal
requirements
TECHNICAL TIP |
Temperature: Electrophoresis at
high voltages produces heat.
Additionally, high-conductivity buffers such as
TAE generate more heat than low-conductivity
buffers. Care should be taken in agarose gel
electrophoresis with voltages greater than
175 Volts, as heat build up can generate gel
artefacts such as S-shaped migration fronts,
and in extended electrophoresis runs can
even melt the agarose gel. With high voltage
electrophoresis, the use of low-melting point
agarose gels should be avoided.
Replacement parts and accessories
TECHNICAL SPECIFICATION
Complete System
Maxi-cooled horizontal gel unit with removable casting tray,
including casting gates with integral silicone seals, 2 x 1mm thick,
16-sample combs, coloured loading strips
and buffer recirculation ports
Replacement Parts & Accessories
1 x gel casting tray, 20 x 20cm, including casting gates
with integral silicone seals
2 x buffer recirculation ports
2 x casting gates with integral silicone seals
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
CHU20
HU20-UT
HU-BRP
HU20-CG
HU-SG
HU20-SS
HU20-CS
HU20-GS
PT-0.2
CABLE-4
Unit Dimensions (W x L x H)
27 x 47.5 x 8cm
Gel Size (W x L)
20 x 20cm
Buffer Volume
2200ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
168
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
16 to 42 samples
Comb Slots
6
Cooling Ports
2
Migration Distance Between
Comb Slots
5cm
Recommended
Running Voltage
150 to 225V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
CHU20 Combs
Part No.
HU20-C1-16
*HU20-C1-20MC
HU20-C1-28
*HU20-C1-40MC
HU20-C1.5-16
*HU20-C1.5-20MC
HU20-C1.5-28
*HU20-C1.5-40MC
HU20-C2-16
*HU20-C2-20MC
HU20-C2-28
*HU20-C2-40MC
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1.5
1.5
1.5
1.5
2
2
2
2
16
20
28
40
16
20
28
40
16
20
28
40
8.5
7
5
3
8.5
7
5
3
8.5
7
5
3
3
2
2
2
3
2
2
2
3
2
2
2
35
30
20
13
55
45
30
19
75
60
40
25
*Multi-channel pipette compatible
19
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
ORDERING INFORMATION
Horizontal Gel Units
BENEFITS INCLUDE
• Cooled base - covering the entire 25 x
30cm gel tray - allows separations to be
performed faster and at higher voltage,
without loss of resolution
• Cooling ports connect the cooled
base either to the
laboratory water
supply or the
optional external
chiller (Page 82) for enhanced cooling
• Twelve comb slots - at 2cm intervals
along the tray for rapid, high throughput
separation of a maximum 624 samples
• Multi-channel
pipette compatible
combs - with a
maximum 52sample throughput allow samples to be
loaded quickly and
easily from 96 and
384-well thermal
cyclers
• Combs - colour-coded and heightadjustable - offer complete control over
loading volume and well depth
CHU25 Maxi-Plus
Cooled Horizontal
The CHU25 maxi-plus cooled horizontal gel electrophoresis unit
allows high throughput screening of nucleic acids to be
performed quickly at high voltage without compromising
resolution.
CHU25 CASTING OPTIONS
HU25-CG - casting gates with integral
silicone seals effectively seal the tray
without the need for tape, provided the
silicone gasket faces outwards as
shown
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to
maintain buffer pH
and prevent ionic
gradient formation
• Coloured loading strips - for easy well
detection when loading
CASTING TIP Regardless of
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Side handles - for safe and easy
transportation around the laboratory
20
the casting method, ensure
leak-free casting by lining the
casting unit with agarose and allowing it to
set before pouring the remainder of the
hand-hot agarose mixture i.e. at approx.
50°C
HU25-SS - 25cm long Scie-Plas
Super-Seals offer total versatility in
casting, allowing the gel length to be
tailored to each user’s personal
requirements
TECHNICAL TIP | Separation Performance: Gel concentration, running buffer,
voltage, temperature, nucleic acid conformation and the presence of ethidium
bromide all affect separation results. To establish the progress of double stranded
DNA, ethidium bromide (0.5µg/ml) is often added to the running buffer. The dye’s fluorescent
properties allow the bands to be visualised under a UV lamp. However, ethidium bromide may
slow down the DNA migration rate by approx. 15%. As an alternative, after electrophoresis, the
gel may be stained in an ethidium bromide solution (0.5µg/ml H20) for 15 to 60 minutes and then
viewed or photographed on a UV transilluminator. Note: The staining time should be minimised
to prevent small nucleic acid fragments from diffusing out of the gel. Background fluorescence
of unbound ethidium bromide can be minimised through destaining by soaking the gel for 5
minutes in 0.01M MgCl2, or for 30 minutes in de-ionised water.
Replacement parts and accessories
Complete System
Maxi-plus cooled horizontal gel unit with removable casting tray,
including casting gates with integral silicone seals, 6 x 1mm thick,
26-sample combs, coloured loading strips
and buffer recirculation ports
Part No.
TECHNICAL SPECIFICATION
CHU25
Unit Dimensions (W x L x H)
Replacement Parts & Accessories
1 x gel casting tray, 25 x 30cm, including casting gates
with integral silicone seals
2 x buffer recirculation ports
2 x casting gates with integral silicone seals
1 x silicone gasket, 1 metre
2 x Scie-Plas Super-Seals
36 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
HU25-UT
HU-BRP
HU25-CG
HU-SG
HU25-SS
HU25-CS
HU25-GS
PT-0.2
CABLE-4
CHU25 Combs
Part No.
*HU25-C1-26MC
*HU25-C1-52MC
*HU25-C1.5-26MC
*HU25-C1.5-52MC
*HU25-C2-26MC
*HU25-C2-52MC
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1.5
1.5
2
2
26
52
26
52
26
52
7
3
7
3
7
3
2
1.5
2
1.5
2
1.5
30
13
45
20
60
25
33 x 56 x 9cm
Gel Size (W x L)
25 x 30cm
Buffer Volume
3000ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
624
Combs
6
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
26 to 52 samples
Comb Slots
12
Cooling Ports
2
Migration Distance
Between Comb Slots
2cm
Recommended Running
Voltage
150 to 250V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
*Multi-channel pipette compatible
DO YOU NEED…?
A UV STERILISATION CABINET
A THERMAL CYCLER
A RECIRCULATING CHILLER
A BUFFER RECIRCULATION PUMP
A GEL DOCUMENTATION SYSTEM
SEE PAGE 98
SEE PAGE 99
SEE PAGE 82
SEE PAGE 35
SEE PAGE 96
21
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ORDERING INFORMATION
22
The Vari-gel Range is designed to provide a low cost
electrophoresis solution that not only offers several gel tray
options within one tank but also retains the quality and features
of the popular Green Range horizontal units.
The Vari-gel Range is available in MINI, MIDI and MAXI formats, each format
determined by the width and number of gel-casting trays.
ORDERING INFORMATION
BENEFITS INCLUDE
• Robust acrylic tank construction
• Safety lid with integral power leads
• Shrouded 4mm power output
connectors universally compatible with
modern-day low to medium voltage
power supplies
• Long-life, 99.99 % pure platinum
Complete System
MINI
A 7cm wide Vari-gel unit, which includes two gel-casting trays:
7 x 7cm and 7 x 10cm (W x L)
Part No.
SVG-SYS
MIDI
A 10cm wide Vari-gel unit, which includes three gel-casting trays:
10 x 7cm, 10 x 10cm and 10 x 15cm (W x L)
TVG-SYS
MAXI
A 15cm wide Vari-gel unit, which includes four gel-casting trays:
15 x 7cm, 15 x 10cm, 15 x 15cm and 15 x 20cm (W x L)
VG-SYS
electrodes
• Gold-plated terminals with corrosionfree stainless steel washers
• Durable high-impact polystyrene
(HIPS) combs
• UV-transparent acrylic gel-casting
Each unit is fabricated from 5mm acrylic sheet as standard and comprises two combs,
buffer recirculation ports, gel trays with aluminium casting gates and platinum
electrodes 0.2mm in diameter.
trays
• Offset asymmetric electrodes
• Colour-coded combs corresponding to
1, 1.5 and 2mm thicknesses
23
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The Vari-gel Range
Horizontal Gel Units
BENEFITS INCLUDE
• Two gel trays - in 7 x 7 and 7 x 10cm (W
x L) sizes, each with aluminium casting
gates
• Multiple comb slots - regularly
positioned along the gel tray and
corresponding to each comb thickness allow the user to adjust the sample
migration distance according to their
own personal preference
SVG-SYS Vari-gel MINI System
The SVG-SYS Vari-gel mini system is ideal for standard
preparatory and analytical electrophoresis techniques.
• Colour-coded combs - 1, 1.5 and 2mm
thick
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to maintain buffer pH
and prevent ionic gradient
formation
SVG-SYS CASTING OPTIONS
SVG-GT-ALCG - aluminium casting
gates at each end of the gel tray
guarantee leak-free casting when they
are first lined with agarose
• Coloured loading
strips - for easy well detection
when loading
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Scie-Plas Super-Seals - available as an
accessory for flexible casting
24
CASTING TIP Regardless of
the casting method, ensure
leak-free casting by lining the
casting unit with agarose and allowing it to
set before pouring the remainder of the
hand-hot agarose mixture i.e. at approx.
50°C
SVG-SS - 7cm long Scie-Plas SuperSeals offer total versatility in casting,
allowing the gel length to be tailored to
each user’s personal requirements
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Complete System
A complete 7cm wide Vari-gel system with 2 removable gel trays,
7 x 7 and 7 x 10cm (W x L); 2 x 1mm, 15-sample combs;
aluminium casting gates and coloured loading strips
Replacement Parts & Accessories
1 x gel casting tray, 7 x 7cm, including aluminium casting gates
1 x gel casting tray, 7 x 10cm, including aluminium casting gates
2 x buffer recirculation ports
2 x aluminium casting gates
2 x Scie-Plas Super-Seals
6 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
SVG-SYS
SVG-GT-7
SVG-GT-10
HU-BRP
SVG-GT-ALCG
SVG-SS
SVG-CS
SVG-GS
PT-0.2
CABLE-4
Unit Dimensions (W x L x H)
25 x 11 x 7cm
Gel Size (W x L)
see Trays
Buffer Volume
350ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
48
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
8 to 20 samples
Recommended Running
Voltage
75 to 100V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV222
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1.5
1.5
1.5
1.5
2
2
2
2
8
12
15
16
8
12
15
16
8
12
15
16
5.5
3
2
1.75
5.5
3
2
1.75
5.5
3
2
1.75
2
2
2
2
2
2
2
2
2
2
2
2
27
15
10
8
40
22
15
12
54
30
20
16
SVG-C1-8
SVG-C1-12
SVG-C1-15
*SVG-C1-16MC
SVG-C1.5-8
SVG-C1.5-12
SVG-C1.5-15
*SVG-C1.5-16MC
SVG-C2-8
SVG-C2-12
SVG-C2-15
*SVG-C2-16MC
DO YOU NEED…?
AGAROSE
A GEL LEVELING TABLE
A POWER SUPPLY
SUPER-SEALS™
SEE PAGE 118
SEE PAGE 34
SEE PAGES 91-92
SEE PAGE 33
*Multi-channel pipette compatible
Migration distances between comb slots,
for either 1mm or 1.5 and 2mm combs, in
the SVG-SYS gel-casting trays
TRAY OPTIONS
Part No.
Gel Size (W x L)
Combs Slots
Migration Distance
Between Comb Slots
Maximum Sample Capacity
*each tray includes 2 x aluminium casting gates
Tray 1
SVG-GT-7
7 x 7cm
4
Tray 2
SVG-GT-10
7 x 10cm
6
(2 slots for 1mm combs;
2 slots for 1.5 and 2mm combs)
(3 slots for 1mm combs;
3 slots for 1.5 and 2mm combs)
2.8cm
32
3.0cm
48
SVG-GT-7
2.8cm
1mm
1.5 / 2mm
2.8cm
SVG-GT-10
3.0cm
1mm
1.5 / 2mm
3.0cm
25
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SVG-SYS Combs
Horizontal Gel Units
BENEFITS INCLUDE
• Three gel trays - in 10 x 7, 10 x 10 and
10 x 15cm (W x L) sizes, each with
aluminium casting gates
• Multiple comb slots - regularly
positioned along the gel tray and
corresponding to each comb thickness allow the user to adjust the sample
migration distance according to their
own personal preference
• Colour-coded combs - 1, 1.5 and 2mm
thick
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to
maintain buffer pH
and prevent ionic
gradient formation
• Coloured loading strips - for easy well
detection when loading
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Scie-Plas Super-Seals - available as an
accessory for flexible casting
TVG-SYS Vari-gel MIDI System
The TVG-SYS Vari-gel midi system is ideal for standard
preparatory and analytical electrophoresis techniques.
Migration distances between comb slots, for either 1mm or 1.5 and 2mm combs,
in the TVG-SYS gel-casting trays
TVG-GT-10
TVG-GT-7
2.8cm
3.0cm
1mm
1.5 / 2mm
1.5 / 2mm
2.8cm
3.0cm
TVG-GT-15
3.9cm
1mm
1.5 / 2mm
3.9cm
TRAY OPTIONS
Part No.
Gel Size (W x L)
Combs Slots
Tray 1
TVG-GT-7
10 x 7cm
4
Tray 2
TVG-GT-10
10 x 10cm
6
Tray 3
TVG-GT-15
10 x 15cm
8
(2 slots for 1mm combs;
(3 slots for 1mm combs;
(4 slots for 1mm combs;
2 slots for 1.5 and 2mm combs) 3 slots for 1.5 and 2mm combs) 4 slots for 1.5 and 2mm combs)
Migration Distance
Between Comb Slots
Maximum Sample Capacity
2.8cm
40
*each tray includes 2 x aluminium casting gates
26
1mm
3.0cm
60
3.9cm
80
ORDERING INFORMATION
Replacement Parts & Accessories
1 x gel casting tray, 10 x 7cm, including aluminium casting gates
1 x gel casting tray, 10 x 10cm, including aluminium casting gates
1 x gel casting tray, 10 x 15cm, including aluminium casting gates
2 x buffer recirculation ports
2 x aluminium casting gates
2 x Scie-Plas Super-Seals
10 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
TVG-SYS
TVG-GT-7
TVG-GT-10
TVG-GT-15
HU-BRP
TVG-GT-ALCG
TVG-SS
TVG-CS
TVG-GS
PT-0.2
CABLE-4
TVG-SYS Combs
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
2
2
2
2
2
8
12
15
16
20
8
12
15
16
20
8
12
15
16
20
9.25
5.5
4
3.62
2.5
9.25
5.5
4
3.62
2.5
9.25
5.5
4
3.62
2.5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
45
25
20
15
10
65
35
30
20
15
90
50
40
30
20
TVG-C1-8
TVG-C1-12
TVG-C1-15
*TVG-C1-16MC
TVG-C1-20
TVG-C1.5-8
TVG-C1.5-12
TVG-C1.5-15
*TVG-C1.5-16MC
TVG-C1.5-20
TVG-C2-8
TVG-C2-12
TVG-C2-15
*TVG-C2-16MC
TVG-C2-20
*Multi-channel pipette compatible
TECHNICAL TIP | Caution:
Ethidium bromide is a known mutagen.
Always wear gloves when handling. Wear UV
safety goggles and protect skin when using any
UV light source.
TECHNICAL SPECIFICATION
Unit Dimensions (W x L x H)
31.5 x 14.5 x 7cm
Gel Size (W x L)
see Trays
Buffer Volume
650ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
80
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
8 to 20 samples
Recommended Running
Voltage
75 to 125V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV222
TVG-SYS CASTING OPTIONS
TVG-GT-ALCG - aluminium casting gates
at each end of the gel tray guarantee leakfree casting when they are first lined with
agarose
TVG-SS - 10cm long Scie-Plas SuperSeals offer total versatility in casting,
allowing the gel length to be tailored to
each user’s personal requirements
27
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Complete System
A complete 10cm wide Vari-gel system with 3 removable gel trays,
10 x 7, 10 x 10 and 10 x 15cm (W x L); 2 x 1mm, 15-sample combs;
aluminium casting gates and coloured loading strips
Horizontal Gel Units
BENEFITS INCLUDE
• Four gel trays - in 15 x 7, 15 x 10, 15 x 15
and 15 x 20cm (W x L) sizes, each with
aluminium casting gates
• Multiple comb slots - regularly
positioned along the gel tray and
corresponding to each comb thickness allow the user to adjust the sample
migration distance according to their
own personal preference
VG-SYS Vari-gel MAXI System
The VG-SYS Vari-gel maxi system is ideal for standard
preparatory and analytical electrophoresis techniques.
Migration distances between comb slots, for either 1mm or 1.5 and 2mm combs, in
the VG-SYS gel-casting trays
• Colour-coded combs - 1, 1.5 and 2mm
thick
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis to maintain buffer pH
and prevent ionic gradient formation
• Coloured loading
strips - for easy
well detection when loading
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
• UV-transparent acrylic casting tray allows the user to image the gel without
risk of damage due to handling
• Scie-Plas Super-Seals - available as an
accessory for flexible casting
VG-GT-7
2.8cm
VG-GT-10
1mm
1.5 / 2mm
2.8cm
3.0cm
1mm
1.5 / 2mm
3.5cm
VG-GT-20
3.1cm
1mm
1.5 / 2mm
3.1cm
28
1mm
1.5 / 2mm
VG-GT-15
3.5cm
3.0cm
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Replacement Parts & Accessories
1 x gel casting tray, 15 x 7cm, including aluminium casting gates
1 x gel casting tray, 15 x 10cm, including aluminium casting gates
1 x gel casting tray, 15 x 15cm, including aluminium casting gates
1 x gel casting tray, 15 x 20cm, including aluminium casting gates
2 x buffer recirculation ports
2 x aluminium casting gates
2 x Scie-Plas Super-Seals
12 x coloured loading strips
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded
4mm power output connectors
Part No.
VG-SYS
VG-GT-7
VG-GT-10
VG-GT-15
VG-GT-20
HU-BRP
VG-GT-ALCG
VG-SS
VG-CS
VG-GS
PT-0.2
Unit Dimensions (W x L x H)
31.5 x 19 x 7cm
Gel Size (W x L)
see Trays
Buffer Volume
950ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
180
Combs
2
Comb Thickness
1, 1.5 or 2mm
Comb Throughput
10 to 30 samples
Recommended Running
Voltage
75 to 150V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
CABLE-4
VG-SYS Combs
Part No.
Thickness
(mm)
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
2
2
2
2
2
10
16
20
25
30
10
16
20
25
30
10
16
20
25
30
12.8
6.4
5.9
3.5
2.5
12.8
6.4
5.9
3.5
2.5
12.8
6.4
5.9
3.5
2.5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
64
32
29
17
12
96
48
43
25
18
128
64
58
34
24
VG-C1-10
*VG-C1-16MC
VG-C1-20
VG-C1-25
*VG-C1-30MC
VG-C1.5-10
*VG-C1.5-16MC
VG-C1.5-20
VG-C1.5-25
*VG-C1.5-30MC
VG-C2-10
VG-C2-16MC
VG-C2-20
VG-C2-25
*VG-C2-30MC
*Multi-channel pipette compatible
TRAY OPTIONS
Part No.
Gel Size (W x L)
Combs Slots
Tray 1
VG-GT-7
15 x 7cm
4
(2 slots for 1mm combs;
2 slots for 1.5 and
2mm combs)
Migration Distance
Between Comb Slots 2.8cm
Maximum Sample
Capacity
60
Tray 2
VG-GT-10
15 x 10cm
6
Tray 3
VG-GT-15
15 x 15cm
8
Tray 4
VG-GT-20
15 x 20cm
12
3.0cm
3.5cm
3.1cm
90
120
180
(3 slots for 1mm combs;
3 slots for 1.5 and
2mm combs)
(4 slots for 1mm combs;
4 slots for 1.5 and
2mm combs)
ELECTROPHORESIS
CHEMICALS
SEE PAGES 117-123
(6 slots for 1mm combs;
6 slots for 1.5 and
2mm combs)
*each tray includes 2 x aluminium casting gates
29
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Complete System
A complete 15cm wide Vari-gel system with 4 removable gel trays,
15 x 7, 15 x 10, 15 x 15 and 15 x 20cm (W x L); 2 x 1mm,
15-sample combs; aluminium casting gates and
coloured loading strips
Horizontal Gel Units
BENEFITS INCLUDE
• Single gel tray with fluorescent
numbering - allows up to 96 SSPs to be
loaded directly onto the tray, so that each
SSP is automatically numbered for gel
photography, simplifying postelectrophoretic analysis
• 6 comb slots - positioned at 25mm
intervals along the gel tray allow SSPs to
be separated in less than 30 minutes
using the appropriate running conditions
(see Technical Specification)
• Six 17-sample combs - with each
marker lane positioned centrally enables SSPs to be loaded onto the gel
quickly, 8 at a time by multi-channel
pipette
• Compact tank - reduces the buffer
volume required to cover the gel,
providing greater control over the voltage
gradient and run-time
VG-FAST96 High Throughput
Vari-gel MAXI System
The VG-FAST96 high throughput Vari-gel maxi system is the
latest addition to the Scie-Plas Vari-gel product range, providing
a swift and simple solution for the screening and analysis of up
to 96 single-strand polymorphisms (SSP) following PCR®
amplification. Under the appropriate running conditions, SSPs,
50 to 1000 base pairs in size, can be separated in less than 30
minutes.
• Robust acrylic casting tray - UVtransparent for visualisation with
fluorescent numbering for SNP
recognition and traceability
• Ideal for use - either with 96-well
microtitre plates or 96-well blocks
• Buffer recirculation ports - may be
connected to a peristaltic pump for
buffer recirculation during
electrophoresis
30
ROBUST ACRYLIC
CASTING TRAY
Fluorescent visualisation of the
lanes for the markers and
samples 1-96 on the VG-FAST-GT
high throughput gel-casting tray,
using the Scie-Plas Vision gel
documentation system.
ORDERING INFORMATION
Complete System
A complete 16cm wide high throughput Vari-gel system
with 1 removable UV-transparent and UV-fluorescent gel tray,
16 x 17.5cm (W x L); 6 x 1mm, 17-sample combs and
aluminium casting gates
Part No.
VG-FAST96
Replacement Parts & Accessories
1 x removable UV-transparent and UV-fluorescent gel casting tray,
16 x 17.5cm, including aluminium casting gates
2 x aluminium casting gates
2 x buffer recirculation ports
1 x gel scoop
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with
shrouded 4mm power output connectors
VG-FAST-GT
VG-FAST-ALCG
HU-BRP
VG-FAST-GS
PT-0.2
CABLE-4
VG-FAST 96 Combs
Part No.
Thickness
(mm)
VG-FAST-C1-17MC
1
*Multi-channel pipette compatible
Sample
Throughput
Tooth
Width (mm)
Max.
Spacing (mm)
Sample Volume
in a 5mm
Deep Well (µl)
17
5
3.5
25
Unit Dimensions (W x L x H)
33 x 19.5 x 7.5cm
Gel Size (W x L)
16 x 17.5cm
Buffer Volume
950ml
Buffer Recirculation Ports
2
Maximum Sample Capacity
96 + 8
Combs
6
Comb Thickness
1mm
Comb Throughput
17 samples:
2 x 8 loaded by
multi-channel
pipette;
1 x marker
Comb Slots
6
Migration Distance
Between Comb Slots
2.5cm
Recommended
Running Voltage
100 to 150V
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
TECHNICAL TIP |
Photography:
If the gel needs to be photographed,
thin gels (2 to 3mm) with low-percentage
agarose are better than thick or high-percentage
gels. The latter produce increased opaqueness
and autofluorescence.
31
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TECHNICAL SPECIFICATION
Horizontal Gel Units
Today’s laboratory scientists can take
advantage of many gel-casting methods,
ranging from the traditional, homemade
solution of sealing the ends of the gel tray
with tape to flexible gel casting, at any
position within the tray, with Scie-Plas
Super-Seals™.
SCIE-PLAS CASTING OPTIONS
Plastic casting gates
• Available with the HB-SET and H1SET units only
• Slot into preformed grooves within
the gel tank
Aluminium casting gates
• Available with the SVG-SYS,
TVG-SYS and VG-SYS units only
• Slot into preformed grooves within
the gel-casting tray
Silicone gasket
• Available with the HU6, HU10 and
HU13 units only
• Slot into preformed grooves at each
end of the gel-casting tray, which is
then turned at 90° to the direction
of electrophoresis to form a leak-free seal against the
walls of the gel tank
Casting gates with
integral silicone seals
• Available with the HU15, HU20,
HU25, CHU20 and CHU25 units
• By slotting into preformed
grooves, casting gates with
integral silicone seals effectively
seal the tray, provided the silicone gasket faces
outwards as shown
Horizontal Gel Casting
Products
Scie-Plas currently provides a variety of gel-casting options with its horizontal
units, all of which will ensure reliable casting.
Always line the casting
gates with a small amount
of agarose, allowing it to set
before pouring the remainder of the
cooled, hand-hot agarose mixture i.e.
at approx. 50°C.
ORDERING INFORMATION
Description
2 x plastic casting gates for the HB-SET
2 x plastic casting gates for the H1-SET
Part No.
HB-CG
H1-CG
N.B. Casting only performed directly in the unit, without casting trays
Description
2 x aluminium casting gates for the SVG-SYS
2 x aluminium casting gates for the TVG-SYS
2 x aluminium casting gates for the VG-SYS
Part No.
SVG-GT-ALCG
TVG-GT-ALCG
VG-GT-ALCG
N.B. For individual gel trays with aluminium casting gates, please see the SVG-SYS,
TVG-SYS and VG-SYS product literature.
Description
Part No.
1 x silicone gasket for the HU6, HU10 & HU13, 1 metre HU-SG
N.B. For individual gel trays with silicone gaskets, please see the HU6, HU10 and
HU13 product literature.
Description
2 x casting gates with integral silicone seals for HU15
2 x casting gates with integral silicone seals for CHU20
& HU20
2 x casting gates with integral silicone seals for CHU25
& HU25
Part No.
HU15-CG
HU20-CG
HU25-CG
N.B. For individual gel trays with casting gates with integral silicone seals,
please see the respective product literature for each unit
Silicone casting gates
• Available for the HU6, HU10
and HU13 units
• Slot into grooves at each end of
the casting tray to form a leak-free seal
Description
2 x silicone casting gates for HU6
2 x silicone casting gates for HU10
2 x silicone casting gates for HU13
Part No.
HU6-SCG
HU10-SCG
HU13-SCG
External casting units
• Available for the HU6, HU10 and
HU13 units for simultaneous
casting of up to 3 gels
• Silicone gaskets, seated in the
groove of each casting tray, form a leak-free seal
against the walls of the casting unit
Description
1 x external casting unit for 3 gel trays, HU6
1 x external casting unit for 3 gel trays, HU10
1 x external casting unit for 3 gel trays, HU13
Part No.
HU6-CU
HU10-CU
HU13-CU
32
How to cast with Scie-Plas Super-Seals™
1. Place each Scie-Plas Super-Seal™ at the
desired position within the gel tray,
applying an even pressure along its length
Throw away all those gel gates
and casting units and use our
specifically designed and
formulated silicone seals,
which can be placed anywhere
within the casting tray.
• Cost-effective and completely versatile can be positioned anywhere within the
casting tray, allowing the user to generate
shorter gel lengths without the need for
smaller casting trays
• Reliable, leak-free casting - is a
2. Line each Scie-Plas Super-Seal™ with a few
millilitres of melted agarose, allowing it to
cool and then set to form a leak-proof seal
certainty, provided the seals are first
inserted correctly within the tray and then
lined with agarose before pouring the
remainder of the cooled gel mixture
• Still effective - even with boiling agarose
- as long as the seals are inserted
properly and lined with agarose
Scie-Plas Super-Seals™ offer
• Available - for all Scie-Plas horizontal
the ultimate solution in flexible, gel-casting trays
leak-free gel casting.
3. Pour the remainder of the agarose mixture
- preferably cooled to hand-heat (i.e. at
50°C) for completely reproducible results into the gel tray
ORDERING INFORMATION
Description
Approximate
Dimensions
(L x W x H)
Part No.
Green Range
2 x 6cm long Super-Seals for the HU6
2 x 10cm long Super-Seals for the HU10
2 x 13cm long Super-Seals for the HU13
2 x 15cm long Super-Seals for the HU15
2 x 20cm long Super-Seals for the CHU20 & HU20
2 x 25cm long Super-Seals for the CHU25 & HU25
6 x 1 x 1cm
10 x 1.1 x 1cm
13 x 1.1 x 1.4cm
15 x 1.1 x 1.4cm
20 x 2 x 1.2cm
25 x 2 x 1.2cm
HU6-SS
HU10-SS
HU13-SS
HU15-SS
HU20-SS
HU25-SS
Vari-gel Range
2 x 7cm long Super-Seals for the SVG-SYS
2 x 10cm long Super-Seals for the TVG-SYS
2 x 15cm long Super-Seals for the VG-SYS
7 x 1 x 1cm
10 x 1.1 x 1cm
15 x 1.1 x 1.4cm
SVG-SS
TVG-SS
VG-SS
4. Insert combs and allow the gel to set for at
least 30 minutes before removing the
comb and loading the samples
Tip: Scie-Plas recommends
0.67ml of agarose per cm2 of
gel tray area for a minimum
5mm thick gel - e.g. 150ml of melted
agarose for a 15 x 15cm gel tray, as
shown (Volume ml = W x L x 0.67).
33
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Scie-Plas
Super-Seals™
BENEFITS INCLUDE
Horizontal Gel Units
BENEFITS INCLUDE
Gel Leveling Table
Allows advance pouring of gels of
uniform thickness.
• Large platform - 30 x 20cm platform area
accommodates all Scie-Plas gel-casting
trays from the HU6 to HU25 units
• Easy to use - large, thumb-wheel leveling
feet at each corner facilitate easy
adjustment of the platform
ORDERING INFORMATION
Description
1 x gel leveling table, 30 x 20cm
Part No.
GLT-3020
• Leveling bubble - in the centre of the
platform ensures even gel-pouring
Coloured Strips
Aid sample visualisation during gel loading.
BENEFITS INCLUDE
• In 3 different colours for easy visualisation of
samples mixed with
different dyes such
as bromophenol
and xylene
cyanol
• Adhesive - each
colour strip has an
adhesive backing
allowing it to be
easily affixed and
removed with each
application
ORDERING INFORMATION
Description
Part No.
Green Range
12 x coloured loading strips for HU6
12 x coloured loading strips for HU13
12 x coloured loading strips for HU15
12 x coloured loading strips for HU20 and CHU20
36 x coloured loading strips for HU25 and CHU25
HU6-CS
HU13-CS
HU15-CS
HU20-CS
HU25-CS
Vari-gel Range
6 x coloured loading strips for SVG-SYS
10 x coloured loading strips for TVG-SYS
12 x coloured loading strips for VG-SYS
SVG-CS
TVG-CS
VG-CS
• Available - for all horizontal units
Gel Scoops
Ideal for safe and easy transfer of gels from staining or
destaining tanks to a transilluminator or similar surface.
ORDERING INFORMATION
Description
34
Scoop Area Part No.
(W x L)
Green Range
1 x gel scoop for HU6
1 x gel scoop for HU10
1 x gel scoop for HU13
1 x gel scoop for HU15
1 x gel scoop for HU20 and CHU20
1 x gel scoop for HU25 and CHU25
6 x 8.5cm
10 x 14.5cm
13 x 15cm
15 x 15cm
20 x 25cm
25 x 32.5cm
Vari-gel Range
1 x gel scoop for SVG-SYS
1 x gel scoop for TVG-SYS
1 x gel scoop for VG-SYS
7 x 10cm
10 x 15cm
15 x 20cm
HU6-GS
HU10-GS
HU13-GS
HU15-GS
HU20-GS
HU25-GS
SVG-GS
TVG-GS
VG-GS
BENEFITS INCLUDE
• High flow rate - single channel flows up
to 0.01 to120ml/min
• Highly compact - with single control
potentiometer for direction, speed and
start/stop
• Analogue speed control and remote
switching via a 15D connector
• Reversible - for easy fluid recovery
• Continuous tubing
Buffer Recirculation Pump
The 401/D1 auto/manual control ultra compact buffer
recirculation pump is ideal for those users wishing to
maintain buffer recirculation during horizontal gel
electrophoresis. The pump can be connected to the HU13,
HU15, CHU20, HU20, CHU25, HU25, SVG-SYS, TVG-SYS,
VG-SYS and COMET units through buffer recirculation ports
located within the lid of each unit.
ORDERING INFORMATION
Description
401U/D1 (15VAC) 220-240V 50/60Hz
1ph single channel standard tubing pump
Weight
1.0kg
Dimensions (W x H x D)
80 x 84 x 133mm
Operational
Temperature Range
5°C to 40°C
Noise
<70dBA at 1m
Control ratio
10:1
Standards
IEC335-1, EN60529 (IP21)
CE, BS0800,
Supply
15 Vac from
mains
transformer
Materials of Construction
Rpm
Part No.
20-200
040.1S1D.020
Replacement Parts & Accessories
1 metre platinum silicone tube, bore 4.0mm / wall 1.6mm
TECHNICAL SPECIFICATION
913.A040.016
Drive
Extruded
anodised
aluminium
Front Support
Anodised
aluminium plate
Pumphead Track
Extruded
anodised
aluminium
Pumphead Rollers
Acetal
Pumphead Rotor
Aluminium
35
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• Adjustable occlusion (pressure)
Specialist Gel Units
BENEFITS INCLUDE
• Developed in collaboration with endusers - as with all our gel
electrophoresis units, the CA-SYS and
units have been designed in close
consultation with end-users: in this case
NHS biomedical scientists who routinely
use cellulose acetate electrophoresis to
screen for mucopolysaccharide (MPS)
disorders in children
• Active tank area: -
CA-SYS Cellulose
Acetate System
The CA-SYS cellulose acetate system has been designed in close
collaboration with the UK National Health Service (NHS) to run
cellulose acetate membranes and wick-based electrophoresis
applications, particularly in the two-dimensional screening of
inherited metabolic disorders in children.
CA-SYS - 24.5 x 26cm (W x L) easily
accommodates larger 145 x 192mm
membranes, or up to three standard
78 x 75/150mm membranes
simultaneously
• Adjustable bridge prices - can be
located anywhere within the tank to
support cellulose acetate membranes or
horizontal precast gels
• Clamping bars within clip holders hold the cellulose acetate membrane or
paper wick gently in position
• Asymmetric offset electrodes - ensure
that the membrane is run in the correct
polar orientation
• Transparent lid - allows electrophoresis
to be monitored as it happens as well as
maintaining uniform atmospheric
conditions
• A central partition - separates the
buffer and lends additional support to
longer membranes and precast gels
• Membranes available - in small,
standard and large formats
36
Asymmetric offset electrodes ensure that the membrane is run in
the correct polar orientation
Active tank area CA-SYS - accommodates up to
three small/standard 78 x
75/150mm membranes or one
large 145 x 192mm membrane
Clamping bars within clip holders hold the cellulose acetate membrane
or paper wick gently in position
Adjustable bridge prices - can be
located anywhere within the tank to
support cellulose acetate membranes
or horizontal precast gels
Central partition - separates
the buffer and lends additional
support to longer membranes
and precast gels
Outline Protocol for Two-dimensional (2-D)
Electrophoresis of Urinary
Glycosaminoglycans - a Diagnostic
Indicator of Mucopolysaccharide
Disorders
Buffer 1: Pyridine: Acetic Acid: Distilled
Water (10:1:89 v/v)
Applications Include:
Buffer 2: 0.1M Barium Acetate, pH 6.0
• Serum Proteins
• Lipoproteins
• Haemoglobin
• Metabolites in Urine and Spinal Fluids
Stain: Alcian Blue
Destain: 5% Acetic Acid (v/v)
i. Spot 2 x 1µl aliquots of extracted urine
sample, stained with Alcian Blue, onto a
78 x 75mm cellulose acetate
membrane, previously soaked and
electrophoresed in Buffer 1 for 10
minutes at 55V (7.5V/cm) using either
the CA-SYS or system.
CA-SYS
Unit Dimensions (W x L x H)
30 x 32 x 6.5cm
Active Tank Dimensions (W x L x H)
24.5 x 26 x 3.5cm
Recommended Buffer Volume (ml)
1200ml
Maximum Membrane Length (mm)
230mm
Recommended Power Supply
55V (10mA)
Power Output Connectors (diameter)
Shrouded, 4cm
Recommended Power Supplies
Consort EV243
ORDERING INFORMATION
CA-SYS
Complete System
Cellulose acetate system, including 30 x 32cm tank with central
partition, 2 x adjustable bridge pieces with clamping bars,
transparent lid and 4mm power output connectors
Part No.
CA-SYS
Replacement Parts & Accessories
2 x adjustable bridge pieces with clamping bars, 27cm in length
CA-ABS
2 x 1 metre power leads with shrouded 4mm power output connectors CABLE-4
Platinum Wire - 2 x 0.2mm thick platinum electrode wire
PT-0.2
Membranes
100 x ATX-micro solid standard, small-format cellulose
acetate membranes, 78 x 75mm
50 x ATX-micro solid standard, medium-format cellulose
acetate membranes, 78 x 150mm
50 x ATX-micro solid large-format cellulose acetate membranes,
145 x 192mm
ii. Run the membrane at 55V for a further
75 minutes in Buffer 1.
iii. Remove
the
cellulose
acetate
membrane from the CA-SYS or system,
allowing it to dry in a fume cupboard for
1 hour.
iv. Float the cellulose acetate membrane
face down for 5 seconds in Buffer 2,
before rotating it at 90° to its original
orientation and then spotting a 0.5µl
aliquot of MPS Type III urine as a
positive control.
v. Dry and then electrophorese the
cellulose acetate membrane at 55V for
31/2 hours.
vi. Stain the cellulose acetate membrane
face down for 15 minutes, before rinsing
it in water and standing it overnight in
Destain.
vii. After destaining, rinse the cellulose
acetate membrane with water and dry it
flat between two sheets of filter paper.
Part No.
CA-MEM-S
CA-MEM-M
CA-MEM-L
Diagnostic Analysis of Mucopolysaccharide
Disorders with the CA-SYS unit. Extracted
urine sample was applied to a 78 x 75mm
cellulose acetate membrane, electrophoresed at 55V for 75 minutes in the first
dimension, and then electrophoresed in the
second dimension for a further 31/2 hours at
same voltage as described in the Outline
Protocol. (Courtesy of the NHS)
37
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TECHNICAL SPECIFICATION
Specialist Gel Units
COMET Single Cell Gel
Electrophoresis Systems
BENEFITS INCLUDE
• Available in 2 formats - to
accommodate 20 or 40 standard
microscope slides respectively
• Opaque ebony acrylic tank and lid -
The COMET single cell gel electrophoresis systems are designed
specifically for single cell gel electrophoresis (SCGE). Comet
Assays are used to detect and quantify DNA damage and repair
within individual cells in genetic toxicology and carcinogenesis
studies.
provide ideal protection for techniques
requiring light-sensitive stains or
samples
• Cooled central platform - provides a
convenient surface for slide preparation
in addition to maintaining slide
temperature during pre-incubation and
electrophoresis (see below)
Buffer recirculation ports maintain buffer circulation,
preventing ion gradient
formation
• Cooling ports - can be connected to an
external chiller unit: to prevent
overheating during SCGE/Comet assays,
typically performed at high current
settings over 300mA; and to inhibit DNA
repair enzyme activity by maintaining the
slide temperature at 4°C, either during
preparation and mounting or preincubation and electrophoresis
Opaque ebony acrylic tank and lid provide ideal protection for techniques
requiring light-sensitive stains or
samples
• Buffer recirculation ports - can be
connected to a peristaltic pump to
maintain buffer circulation, preventing
ion gradient formation
• Colour-coded handles - corresponding
to the anode and cathode - serve as a
visual aid to ensure that the slides are
positioned in the correct polar
orientation for the assay
Cooling ports - can be connected to
the JULABO chiller unit to maintain
slide temperature before and during
electrophoresis
Cooled central platform - provides a
convenient surface for slide
preparation in addition to maintaining
slide temperature, through a
labyrinthine cooling base, during preincubation and electrophoresis
Colour-coded handles - corresponding
to the anode and cathode - serve as a
visual aid to ensure that the slides are
positioned in the correct polar
orientation for the assay
TECHNICAL SPECIFICATION
Unit Dimensions (W x L x H)
COMET-20
COMET-40
31 x 32 x 6.5cm
31 x 47.5 x 6.5cm
Active Tank Dimensions (W x L x H)
27.5 x 21.5 x 3.5cm
27.5 x 37 x 3.5cm
Recommended Buffer Volume (ml)
600ml
800ml
Buffer Recirculation Ports
2
2
Slide Capacity (25 x 75mm; W x L)
20
40
Recommended Running Conditions
5V/cm (300mA)
5V/cm (300mA)
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supply
Consort EV261
Consort EV261
38
THE COMET ASSAY
Overview - The Comet Assay is based on
the principle that strand breakage of
supercoiled duplex DNA reduces the size of
the large genomic DNA molecule from
which these strands are separated or
stretched out by electrophoresis. The high
pH of the alkaline lysis step causes
denaturation, unwinding of the duplex DNA
and the release of alkali labile sites as SSBs.
These then become “comets” as the broken
ends of the negatively charged DNA
molecule migrate towards the anode during
electrophoresis.
Method - Cells, previously exposed to a
genotoxic insult, are suspended in low
melting point agarose (LMP) and embedded
within a thin agarose gel on a microscope
slide. All cellular proteins are then removed
by lysis in detergent solution, when the DNA
is allowed to unwind in the neutal/alkaline
pH conditions of the detergent. The DNA is
electrophoresed and then stained with a
DNA-specific fluorescent dye such as
acridine orange, ethidium bromide,
propidium iodide or 4',6'-diamidino-2phenylindole (DAPI).
Result - Upon staining cellular DNA is
measured for fluorescence, usually with a
microscopy imaging system. The resulting
image resembles a “comet”, with the
cellular DNA segregating into a “head” and
“tail”. The head is composed of largely
intact genomic DNA, while the tail
comprises damaged (SSBs, DSBs) or
fragmented DNA, with the fluorescence
intensity and length of the tail being directly
proportional to the extent of the DNA
damage.
References - 1. Östling, O., and Johanson,
K.J., Microelectrophoretic study of
radiation-induced DNA damages in
individual mammalian cells. Biochem.
Biophys. Res. Commun., 123, 291-8 (1984).
2. Singh, N.P, et al., A simple technique for
quantitation of low levels of DNA damage in
individual cells. Exp. Cell Res., 175, 184-91
(1988).
1. Cells exposed to
genotoxic insults
such as
γ-irradiation
2. Cells with damaged DNA,
having SSBs/DSBs, are
embedded on an agarosecoated slide and lysed
3. Following electrophoresis
and fluorescent staining,
the damaged DNA is seen
to have migrated away
from the intact DNA (the
“head”) to form a
comet “tail”
ORDERING INFORMATION
Comet Single Cell Gel Electrophoresis Systems
Complete System
Comet assay system for twenty 75 x 25mm slides,
including 27.5 x 21.5cm tank and lid made in ebony acrylic
Comet assay system for forty 75 x 25mm slides,
including 27.5 x 37cm tank and lid made in ebony acrylic
COMET-40
Replacement Parts & Accessories
2 x 1 metre power leads with shrouded 4mm
power output connectors
Platinum Wire - 2 x 0.2mm thick platinum electrode wire
CABLE-4
PT-0.2
Chemicals & Reagents
1 x 100g Agarose low melting research grade
1 x 100g Ethylenediamine tetraacetic acid.Na2-salt
1 x 1kg Boric acid analytical grade
1 x 1kg Tris(hydroxymethyl)aminomethane
1 x 250ml Triton® X-100 molecular biology grade
1 x 1kg Sodium dodecyl sulphate pellets
1 x 50g Acridine orange research grade
1 x 5g Ethidium bromide research grade
1 x 25mg Propidium iodide research grade
1 x 10mg 4',6'-diamidino-2-phenylindole.2HCl.H20 (DAPI) analytical grade
11408.03
11280.01
15165.01
37190.02
39795.02
20765.03
10665.02
21238.02
33671.01
18860.01
Part No.
COMET-20
Comet “Head”
comprising intact
genomic DNA
Comet “Tail”
composed of mobile
damaged or
fragmented DNA
39
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Background - The Comet Assay/SCGE was
pioneered by Östling and Johansson (1) in
1984 as a neutral pH assay to quantify
double-stranded DNA breakages (DSBs) in
single cells exposed to γ irradiation. Singh
et al. (2) then modified the assay in 1988 to a
more versatile and sensitive alkaline
method to measure both single- (SSBs) and
double-stranded DNA breakages. Now, by
introducing subtle changes to the assay
conditions (pH/temperature), the Comet
Assay can be tailored to analyse specific
DNA lesions and repair processes.
Horizontal Gel Units
BENEFITS INCLUDE
• Ceramic cooling plate 27 x 27cm (W x L) - can support either…
• One 245 x 125mm or two 125 x
125mm SERVALYT™ PRECOTES™
IEF gels (see page 121);
The IEF-SYS Dedicated
Isoelectric Focusing Unit
The recently redesigned Scie-Plas IEF-SYS unit has been
optimised for isoelectric focusing applications using
IPG BlueStrips and SERVALYT™ PRECOTES™ and
PreNet™ IEF gels,
also available
through Scie-Plas.
• Or up to a maximum of 30 IPG
BlueStrips, each 3mm wide and up
to 24cm in length (see page 122)
• Ceramic cooling plate can also be
connected to an external chiller to
maintain IEF at…
• 4°C for SERVALYT™ PRECOTES™
and PreNet™ IEF gels
TECHNICAL SPECIFICATION
• 20°C for IPG BlueStrips
IEF-SYS Unit
• Acrylic electrode frame - sits directly
on the ceramic cooling plate for IEF with
SERVALYT™ PRECOTES™ and PreNet™
IEF gels
• Glass electrode frame - sits flush with
the ceramic cooling plate for optimal
cooling efficiency and minimises mess
caused by oil application during IEF with
IPG strips
• Positive and negative electrodes made from clear acrylic clip neatly
within the acrylic or glass electrode
frame, allowing the voltage gradient to
be fine-tuned along the entire length of
each IPG strip
• Glass electrode weight - ensures that the
electrodes remain in complete contact
with the positive and negative ends of each
IPG strip or IEF gel during IEF
• 2mm shrouded power output connectors
- compatible with high voltage power
supplies
• IEF tank or cooling plate - can also be
Maximum Sample Capacity
30 x IPG strips
(dimensions ranging from 70 x 3 x 1mm
to 240 x 3 x 1mm [L x W x T])
1 x 245 x 125mm or 2 x 125 x 125mm (W x L)
SERVALYT™ PRECOTES™ IEF gels
Unit dimensions (W x D x H)
inc. cooling coil
46 x 41 x 11.5cm
Inner Tank Dimensions (W x D x H)
37 x 31 x 8.5cm
Glass plate active dimensions (W x L x D)
27 x 27 x 0.5cm
Electrode frame active dimensions (W x L x H)
27 x 27 x 1cm
Cooling plate dimensions (W x L x D)
27 x 27 x 3cm
Glass electrode weight
active dimensions (W x L x H)
20 x 26.5 x 1cm
Adjustable electrodes
active dimensions (W x L x H)
10 x 265 x 13mm
Recommended temperature for cooling plate
during IEF with IPG strips
20°C
Recommended temperature for cooling plate
during IEF with IEF gels
4°C
Power Output Connectors (diameter)
Shrouded, 2mm
Recommended Running Conditions for Voltage Step
IEF with 125mm long SERVALYT™
Voltage (V)
PRECOTES™ IEF gels
Time (min)
1
2
3
4
300
600
1000
1200
1500
2000
20
20
30
30
30
60
Volt-hours
100
200
500
600
750
2000
1
2
3
4
Recommended Running Conditions for Voltage Step
IEF of 7cm IPG strips
Voltage (V)
used for wick-based horizontal gel
electrophoresis
5 6 end of run
150
300
600
1500
3000
330
Time (h)
0.5
0.5
0.5
0.5
2.5
<20
Volt-hours
75
150
300
750
7500
-
1
2
3
4
5 end of run
300
600
1500
3000
330
Recommended Running Conditions for Voltage Step
IEF of 18cm IPG strips
Voltage (V)
Time (h)
Volt-hours
40
5 6 end of run
1
1
1
12.5
<20
300
600
1500
37500
-
Snap-lock Connectors for Cooling Coil Inner Diameter
10mm
Outer Diameter
12mm
Quick-fit Tubing
10mm
Outer Diameter
12mm
Inner Diameter
Shrouded 2mm power
output connectors
compatible with all modern
commercially available high
voltage power supplies
Ceramic cooling plate is
connected to the external chiller
to maintain IEF at 4 or 20°C for
IEF gels or IPG strips
SERVALYT™ PRECOTES™: OUTLINE
PROTOCOL
1. Set the cooling temperature of the
external chiller, connected to the cooling
plate by quick-fit tubing, to 4ºC at least 10
minutes before using the unit.
2. Place the gel directly on top of the area
covered by Bayol F or kerosene and, by
using a pipette or electrophoresis roller
(42991.01), gently disperse any bubbles
underneath the gel.
Acrylic or glass electrode frame
can be adapted for IEF with IEF
gels and IPG strips respectively
4. Position the electrode wicks at either end
of the IEF gel, at least 5mm inside each
edge, so that they are aligned parallel with
the red and black strips at either end of
the gel. The distance between each
electrode should be 10cm for SERVALYT™
PRECOTES™ IEF gels.
5. Place a clean, washed applicator strip
(42915.01) in the middle of the gel.
ORDERING INFORMATION
Complete System
Part No.
Flat-bed isoelectric focusing unit includes running tank, lid,
1 x ceramic cooling plate, 1 x glass electrode frame, 1 x electrode frame,
1 x anode electrode, 1 x cathode electrode,
1 x glass electrode weight,
2 x quick-fit tubes and 2 x 2mm power cables
IEF-SYS
Replacement Parts & Accessories
1 x ceramic cooling plate for IEF-SYS
1 x glass electrode frame for IEF-SYS
1 x electrode frame for IEF-SYS
1 x cathode electrode for IEF-SYS
1 x anode electrode for IEF-SYS
1 x glass electrode weight for IEF-SYS
2 x quick-fit tubes for cooling plate for IEF-SYS
2 x 1 metre power leads with
shrouded 2mm power output connectors
Platinum Wire
IEF-CP
IEF-GF
IEF-EF
IEF-CE
IEF-AE
IEF-GS
TCS-CC
6. Load 10µl of protein sample into each well
with a pipette, reserving at least one well
for a 10µl-aliquot of SERVA Liquid Mix IEF
Markers 3-10 (39212.01).
7. After loading the gel, carefully lower the
glass electrode weight over the anode and
cathode electrodes, so that they remain in
contact with the electrode wicks and gel
during the application.
8. Perform
IEF
according
to
the
recommended running conditions in
Technical Specification. Once IEF is
complete, the SERVALYT™ PRECOTES™ IEF
gel is ready for staining.
CABLE-2
PT-0.2100CM
Protein samples in a SERVALYT™ PRECOTES™
Wide Range pH 3-10 IEF gel following IEF
with the IEF-SYS unit and fixation and
staining with SERVA Violet 17.
41
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Adjustable electrodes
- clip into slots within
the acrylic or glass
electrode frames, while the glass
electrode support slab maintains
uniform contact between the electrode and
paper wicks for the IEF gel or IPG strip
3. Immerse each electrode wick (42988.01)
in its respective electrode solution (SciePlas recommends 100µl of electrode
solution per cm of wick), depending on
whether it is the anode (42984.03) or the
cathode (42986.03). The length of the
electrode wicks should correspond to the
width of the precast gel.
42
Vertical Gel Units
The TV Range
The Scie-Plas 'TV Range' vertical gel electrophoresis units offer
the ultimate standards in innovative design and manufacture
acquired during our 20+ year existence.
The 'TV Range' vertical gel electrophoresis
units are available in a range of different
sizes for applications ranging from routine
SDS-PAGE mini-gels to large format
nucleic acid sequencing and single-strand
conformational polymorphism studies
(SSCP).
As with our 'Green Range'
horizontal gel units, each 'TV Range'
vertical
gel
electrophoresis
unit
incorporates the many design and safety
features recommended to us over the
years by scientists, either from within our
in-house product development team or
laboratories worldwide. Accordingly, each
unit is manufactured and finished to the
highest specification, versatile, easy to
use and fully compliant with European
safety regulations.
43
Vertical Gel Units
FEATURES
1. Tank and Lid
2. Power Output Connectors
1
3. Cooling Coil
4. New-Style Gel-Running Module
5. Dummy Plate
6. Cam-Pin Casting Base
20
7. Silicone Seals
8. Colour-Coded Spacers
9. Colour-Coded Combs
10. Plain Glass Plates - also available
19
with bonded spacers
11. Notched Glass Plates
12. Spacer Aligners
13. Quick-Fit Tubing With Snap-Lock
Connectors
14. Electroblotting Cassette
15. Fibre Pads
14
16. Glass Capillary Tubes
17. Gel Extraction Platform
18
18. Blanking Ports
19. Capillary Gel-Running Module
(CAPGRM)
17
15
20. Electroblotting Gel-Running Module
(EBGRM)
16
BENEFITS INCLUDE
• Robust acrylic tank construction
• Safety lid with integral power leads
• Shrouded 4 and 2mm power output
connectors universally compatible with
modern-day low, medium and high
voltage power supplies
• Long-life, 99.99% pure platinum
electrodes
• Gold-plated terminals with corrosionfree stainless steel washers
• Offset asymmetric electrodes
• A versatile, time-saving gel-running
module (GRM) which supports both gelcasting and electrophoresis
• Durable high-impact polystyrene
(HIPS) and melinex combs which are
colour-coded, corresponding to 0.25,
0.35, 0.75, 1, 1.5 and 2mm thicknesses
• Electroblotting and 2-D capillary gel
modules available for use within the
same tank either as part of a fully
integrated system or as a stand-alone
unit (TV100 & 400 units only)
44
The 'TV Range' vertical gel electrophoresis
units are availabe in a range of different
formats: The TV50 single-plate 10 x 10cm (W x H)
mini-gel unit is a good, cost-effective option
for routine, low throughput separation of
proteins and nucleic acids in self-cast or
commercially available precast gels.
The TV100 twin-plate 10 x 10cm (W x H)
mini-gel unit is, historically, our best
selling vertical gel electrophoresis unit
which is designed for all standard
polyacrylamide gel electrophoresis (PAGE)
applications. This unit is ideal for use with
commercially available precast gels or gels
cast using our new-style GRM and cam-pin
casting base. Here, gel cassettes are
placed within the GRM and then pulled
tightly onto silicone seals by cam pins
located in the casting base. Glass plate
stops situated within the GRM immediately
above the glass plates serve as an
immovable barrier, locking the plates firmly
into position on the casting base as the cam
pins are turned clockwise through 120°.
The gel can then be poured in total
confidence that it will be leak-free. Each
TV100 unit is also available as a cooled
tank option, and can be adapted, either as
a stand-alone unit or as part of a fully
integrated system, with specific modules
for electroblotting and 2-D electrophoresis.
The TV200 twin-plate 20.5 x 10cm (W x H)
wide format mini-gel electrophoresis unit
allows more samples to be run within the
same gel, eliminating the problems that
can be associated with comparing samples
from 2 separate mini-gels. The TV200
offers the same benefits as the TV100,
including a GRM with glass plate
stops and cam-pin casting system, in
addition to a cooled-tank and modular
electroblotting and capillary gel 2-D
electrophoresis options.
4
5
The TV400 twin-plate 20.5 x 20cm (W x H)
maxi-gel unit offers all the benefits of
the TV100 and 200 units, but in a much
larger gel size and with greater
sample throughput. The TV400-GRM also
includes glass plate stops which act in
tandem with the cam-pin casting base to
provide leak-proof casting, whereas an
optional cooled tank format can be
connected either to the laboratory water
supply or external chiller to maintain the
cooling necessary for high quality
resolution.
Specific modules again
allow the TV400 to be adapted either as
stand-alone electroblotting and 2-D
electrophoresis units or as a fully
integrated system.
6
3
7
13
9
11
The TVS1400 single-plate 33 x 41cm (W x
H) large format vertical gel unit allows
more samples to be processed during
each electrophoretic separation.
12
8
10
Format
Options Available
Standard
(i.e. no casting
or cooling)
Casting
only
Cooling
only
Recommended Applications*
Cooling
& casting
PAGE
Electroblotting
2-D
Capillary
TV50 single-plate 10 x 10cm
(W x H) mini-gel
See TV50
TV100 twin-plate 10 x 10cm
(W x H) mini-gel
See TV100
TV100Y
TV100K
TV100YK
TV100, TV100Y,
TV100K, TV100YK
TV200 twin-plate 20.5 x 10cm
(W x H) wide format mini-gel
See TV200
TV200Y
TV200K
TV200YK
TV200, TV200Y,
TV400 twin-plate 20.5 x 20cm
(W x H) maxi-gel
See TV400
TV400Y
TV400K
TV400YK
TV400, TV400Y,
TV400K, TV400YK
TV400YK-EBSYS,
TV400YK-MODSYS
TV400YK-2DSYS,
TV400YK-MODSYS
TVS1400 single-plate 33 x 41cm
(W x H) large format vertical gel
SSCP
DNA
heteroduplex &
Sequencing oligonucleotide
analysis
TV100YK-EBSYS,
TV100YK-2DSYS,
TV100YK-MODSYS TV100YK-MODSYS
SEE TV400-DGGE
See TVS1400
TVS1400
TVS1400
*Where the TV100YK-EBSYS & TV400YK-EBSYS units include PAGE (GRM) and electroblotting (EBGRM) gel-running modules plus casting and cooling
The TV100YK-2DSYS & TV400YK-2DSYS units include PAGE and 2-D capillary gel-running modules (CAPGRM) plus casting and cooling
The TV100YK-MODSYS & TV400YK-MODSYS units include PAGE, electroblotting and 2-D capillary gel-running modules plus casting and cooling
45
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2
Vertical Gel Units
BENEFITS INCLUDE
• Single-plate 10 x 10cm
mini-gel unit - a simple, non-cooled
cost-effective solution for low
throughput PAGE
• Gel casting - TV100-MC2 and TV100MC10 2 and 10 gel multicasters
• Compatible - with standard, 10 x 10cm
(W x H) commercially available precast
gels
• Optional TV100-SGB silicone gasket allows the GRM to be adapted for the
'non-eared' or short glass plates of
major competitor brands
• Float glass plates - 2mm thick guarantee uniform gel thickness and
even sample migration, and with ground
edges to inhibit sample leakage during
casting
• Single screws - for fast electrophoresis
set-up
• Low buffer volumes - within the inner
chamber of the GRM and bottom
reservoir economises buffer
consumption
• Ventilation holes - prevent the build-up
of condensation during electrophoresis
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
• Small footprint area - occupies minimal
bench space
46
The TV50
Single-Plate Mini-Gel
Electrophoresis Unit
The TV50 single-plate 10 x 10cm (W x H) mini-gel unit is the ideal
cost-effective option for routine, low throughput separation of
proteins and nucleic acids in self-cast or commercially available
precast gels.
TECHNICAL SPECIFICATION
ORDERING INFORMATION
Replacement Parts & Accessories
2 x (10 x 10cm) plain glass plates with 0.75mm bonded spacers for TV50
2 x (10 x 10cm) plain glass plates with 1mm bonded spacers for TV50
2 x (10 x 10cm) plain glass plates with 1.5mm bonded spacers for TV50
2 x (10 x 10cm) plain glass plates with 2mm bonded spacers for TV50
2 x (10 x 10cm) plain glass plates for TV50
2 x (10 x 10cm) notched glass plates for TV50
2 x 0.75mm thick spacers for TV50
2 x 1mm thick spacers for TV50
2 x 1.5mm thick spacers for TV50
2 x 2mm thick spacers for TV50
2 x spacer aligners for TV50
1 x caster system for 2 x (10 x 10cm) gradient mini-gels
1 x caster system for 10 x (10 x 10cm) gradient mini-gels
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power output connectors
Part No.
TV50
TV100-PGS0.75
TV100-PGS1
TV100-PGS1.5
TV100-PGS2
TV100-PG
TV100-NG
TV100-S0.75
TV100-S1
TV100-S1.5
TV100-S2
TV100-SA
TV100-MC2
TV100-MC10
PT-0.2
CABLE-4
Unit Dimensions (W x D x H)
21.5 x 12.5 x 13.5cm
Bottom Reservoir Dimensions
(W x D x H)
16.5 x 6 x 3.5cm
Plate Dimensions (W x H x T)
10 x 10 x 0.2cm
Standard Spacer Dimensions
(W x H x T)
1 x 10 x 0.1cm
Active Gel Dimensions (W x H)
8 x 8.5cm
Maximum Sample Capacity
1 x 20
Recommended Buffer Volume
Inner Buffer Chamber
50ml
Lower Buffer Chamber
250ml
Recommended Running Conditions for
Denaturing/Native PAGE Gel
Voltage
50 - 100V
(5 - 10V/cm)
Current
5 -10mA
Time
1.5 - 2.5h
Power Output Connectors
(diameter)
Shrouded, 4mm
Recommended
Power Supply
Consort EV243
TV50 Combs
Part No.
*TV100-C0.75-8MC
TV100-C0.75-10
TV100-C0.75-12
*TV100-C0.75-16MC
TV100-C0.75-20
*TV100-C1-8MC
TV100-C1-10
TV100-C1-12
*TV100-C1-16MC
TV100-C1-20
*TV100-C1.5-8MC
TV100-C1.5-10
TV100-C1.5-12
*TV100-C1.5-16MC
TV100-C1.5-20
*TV100-C2-8MC
TV100-C2-10
TV100-C2-12
*TV100-C2-16MC
*Multi-channel compatible
Thickness
(mm)
Sample
Throughput
Tooth
Width
(mm)
Tooth
Height
(mm)
Max.
Spacing
(mm)
Sample
Volume /
Well (µl)
0.75
0.75
0.75
0.75
0.75
1
1
1
1
1
1.5
1.5
1.5
1.5
1.5
2
2
2
2
8
10
12
16
20
8
10
12
16
20
8
10
12
16
20
8
10
12
16
6
4
3.75
2.5
2
6
4
3.75
2.5
2
6
4
3.75
2.5
2
6
4
3.75
2.5
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
3
3
2.25
2
15
3
3
2.25
2
15
3
3
2.25
2
1.5
3
3
2.25
2
67
45
42
28
22
90
60
56
37
30
120
90
84
55
45
180
120
112
75
Exploded View of a Scie-plas Gel Cassette
Acrylamide
Solution
Comb
Spacer
Plain
glass
plate
Notched
glass
plate
47
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Complete System
Single-plate mini-gel unit with GRM and integral lower
buffer chamber, lid, 2 x (10 x 10cm; W x H) plain glass plates
2 x 1mm spacers, 2 x spacer aligners,
2 x (10 x 10cm) notched glass plates and 1 x 1mm thick 12-sample comb
Vertical Gel Units
BENEFITS INCLUDE
• Twin-plate 10 x 10cm mini-gel unit - a
simple cost-effective solution for PAGE
techniques using either self-cast or
precast gels
• Two versions available: TV100K - cooled
Standard TV100 - non-cooled
Cooled TV100K - a cooling coil in the
bottom of the gel tank for faster
higher voltage electrophoretic
separations
• Enhanced cooling - in the TV100K snap-lock connectors allow the TV100K
to be connected to the external chiller
unit
• Compatible - with
commercially available
10 x 10cm precast gels
• Precast gels - (see page 123)
• Optional TV100-SGB silicone gasket allows the GRM to be adapted for the
'non-eared' or short glass plates of
major alternative brands
• Gel casting - either with tape or the
optional TV100-CB casting base or
TV100-MC2 and TV100-MC10 2 and 10
gel multicasters
TV100 - non-cooled
TV100 Standard &TV100K
Cooled Twin-Plate Mini-Gel
Electrophoresis Units
The TV100 and TV100K twin-plate 10 x 10cm (W x H) mini-gel units
are ideal cost-effective solutions for standard PAGE applications,
using self-cast or commercially available precast gels.
TECHNICAL SPECIFICATION
• Float glass plates - 2mm thick guarantee uniform gel thickness and
even sample migration, and with ground
edges to inhibit sample leakage during
casting
• Single screws - at either side each gelclamping plate - act in conjunction with
a counterbalanced, dovetailed silicone
gasket to disperse pressure evenly along
the height and breadth of the gel as the
screws are tightened
• Low buffer volumes - within the inner
TV100
TV100K
20 x 15 x 14cm
28 x 15 x 18cm
Inner Tank Dimensions (W x D x H)
16.5 x 11 x 11.5cm
16.5 x 11 x 15cm
Plate Dimensions (W x H x T)
10 x 10 x 0.2cm
10 x 10 x 0.2cm
Standard Spacer Dimensions (W x H x T)
1 x 10 x 0.1cm
1 x 10 x 0.1cm
Active Gel Dimensions (W x H)
8 x 8.5cm
8 x 8.5cm
Maximum Sample Capacity
2 x 16
2 x 16
Recommended Buffer Volume
Inner Buffer
Chamber
Unit Dimensions (W x D x H)
Gel Tank
Recommended Running Conditions for
Denaturing/Native PAGE Gel
chamber of the GRM and gel tank
economises buffer consumption
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
• Smaller footprint area - occupies
minimal bench space
48
90ml
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
1200ml
Inner Buffer
Chamber
Gel Tank
Voltage
50 - 100V
(5 - 10V/cm)
Current
5 - 10mA
Current
Time
1.5 - 2.5h
Time
90ml
1600ml
Voltage100 - 150V
(10 - 15V/cm)
10 - 15mA
1 - 1.75h
Inner Diameter
n/a
Inner Diameter
10mm
Outer Diameter
n/a
Outer Diameter
12mm
Inner Diameter
n/a
Inner Diameter
10mm
Outer Diameter
n/a
Outer Diameter
12mm
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supply
Consort EV243
Consort EV243
BENEFITS INCLUDE
• Twin-plate 10 x 10cm mini-gel unit designed specifically for PAGE techniques
requiring self-cast gels, although precast
gels may also be used
• Casting base - included as standard with
both units
TV100YK - cooled + casting
• Two versions available: Standard TV100Y - non-cooled + casting
Cooled TV100YK - cooled + casting
• Glass plate stops - within the newly
modified GRM - lock the glass plates firmly
into position onto soft silicone gaskets in
the casting base to ensure leak-free gelcasting
TV100Y Standard &TV100YK
Cooled Twin-Plate Mini-Gel
Electrophoresis Units
The TV100Y and TV100YK twin-plate 10 x 10cm (W x H) mini-gel
units are ideal for standard PAGE applications requiring self-cast
gels. Soft silicone gaskets in a precision engineered casting
base, included as standard with each unit, act in tandem with our
newly modified GRM to provide simple, leak-free gel casting and
assembly.
TECHNICAL SPECIFICATION
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Plate Dimensions (W x H x T)
Standard Spacer Dimensions (W x H x T)
Active Gel Dimensions (W x H)
Maximum Sample Capacity
Recommended Buffer Volume
Recommended Running Conditions for
Denaturing/Native PAGE Gel
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Casting Base Silicone Seal
Dimensions (W x L x H)
Power Output Connectors (diameter)
Recommended Power Supply
TV100Y
20 x 15 x 14cm
16.5 x 11 x 11.5cm
10 x 10 x 0.2cm
1 x 10 x 0.1cm
8 x 8.5cm
2 x 16
Inner Buffer Chamber 90ml
Gel Tank
1200ml
Voltage
50 - 100V
(5 - 10V/cm)
Current
5 - 10mA
Time
1.5 - 2.5h
Inner Diameter
n/a
Outer Diameter
n/a
Inner Diameter
n/a
Outer Diameter
n/a
1.5 x 11 x 0.8cm
TV100YK
28 x 15 x 18cm
16.5 x 11 x 15cm
10 x 10 x 0.2cm
1 x 10 x 0.1cm
8 x 8.5cm
2 x 16
Inner Buffer Chamber 90ml
Gel Tank
1600ml
Voltage100 - 150V
(10 - 15V/cm)
Current
10 - 15mA
Time
1 - 1.75h
Inner Diameter
10mm
Outer Diameter
12mm
Inner Diameter
10mm
Outer Diameter
12mm
1.5 x 11 x 0.8cm
Shrouded, 4mm
Consort EV243
Shrouded, 4mm
Consort EV243
• Enhanced cooling - in the TV100YK - snaplock connectors allow the cooling coil to be
connected to an external chiller unit for
faster, higher voltage separations
• Compatible - with
commercially available
10 x 10cm precast gels,
with precast gels
available through SciePlas for the TV100-GRM
(see page 123)
• Additional gel casting - with the optional
TV100-MC2 and TV100-MC10 2 and 2 gel
multicaster
• Float glass plates - 2mm thick - guarantee
uniform gel thickness and even sample
migration, and with ground edges to inhibit
sample leakage during casting
• Single screws - at either side each gelclamping plate - act in conjunction with a
counterbalanced, dovetailed silicone gasket
to disperse pressure evenly along the
height and breadth of the gel as the screws
are tightened
• Low buffer volumes - within the inner
chamber of the GRM and gel tank
economises buffer consumption
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
• Smaller footprint area - occupies minimal
bench space
49
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TV100Y - non-cooled + casting
Vertical Gel Units
CASTING WITH NEW-STYLE GRM
1. Place each plain glass plate on a
level bench surface, followed by the
spacers and then the notched glass
plate
2. Insert the glass plate cassette into
the gel-running module (GRM),
placed on its side on the bench,
and lightly tighten the screws
- repeat for the other cassette by
turning the GRM on its other side
3. If not using plain glass plates with
bonded spacers, get a spacer
aligner to align the spacers flush
with the vertical edges of the plain
glass plates before tightening the
screws within the GRM
4. Run your forefinger along the
bottom edge of the glass plates to
ensure that they are flush with
bottom edge of each spacer - if not,
repeat step 3
Silicone seals - form a leak-free
seal as the glass plates are
dragged onto the casting base
Cam pins - turned clockwise to
drag the glass plates firmly onto
the silicone seals within the
casting base
10. Fill the inner buffer chamber
within the GRM so that it just
covers the top of the gel - the gel
is now ready to be loaded
11. Replace the lid and insert the
power cables into the power
supply, making sure that it is first
switched off - the unit is now ready
for electrophoresis
Glass plate stops - serve as an
immoveable barrier, locking the
glass plates firmly into position on
the casting base
5. Lower the assembled GRM onto
the casting base with the cam pins
pointing downwards into the bench
surface
Single screws act, as they are tightened, with a
silicone dovetailed gasket to apply
an even pressure across the height
and breadth of the gel that
prevents the inner gel chamber
from leaking
6. Turn the cam pins through 120° to
secure the GRM onto the casting
base - as you turn the cam pins you
will experience increased resistance
Casting base re-engineered with deeper
recesses to accommodate soft
silicone seals which sit flush with
the bottom of each glass plate
7. Carefully insert the appropriate
comb allowing the gel to set for at
least 30 minutes
8. After carefully removing the
combs, release the GRM by turning
the cam pins in the opposite
direction on the casting base
50
9. Place the GRM into the gel tank,
ensuring that there is sufficient
buffer to cover the bottom of the
glass plates
ORDERING INFORMATION
Part No.
TV100
Twin-plate mini-gel unit with GRM and cooled gel tank,
with built-in cooling coil and quick-fit tubing, plus lid,
2 x (10 x 10cm; W x H) plain glass plates, 4 x 1mm spacers,
2 x spacer aligners, 2 x (10 x 10cm) notched glass plates,
1 x dummy plate and 2 x 1mm thick 12-sample combs
TV100K
Twin-plate mini-gel unit with GRM and gel tank, lid, 2 x (10 x 10cm;
W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners,
2 x (10 x 10cm) notched glass plates, 1 x dummy plate
and 2 x 1mm thick 12-sample combs
plus casting base and 2 x silicone seals
TV100Y
Twin-plate mini-gel unit with GRM and cooled gel tank,
with built-in cooling coil and quick-fit tubing, plus lid, 2 x (10 x 10cm;
W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners,
2 x (10 x 10cm) notched glass plates, 1 x dummy plate,
2 x 1mm thick 12-sample combs, casting base and 2 x silicone seals
TV100YK
Replacement Parts & Accessories
1 x TV100 gel-running module
2 x quick-fit tubes for cooling coil
2 x (10 x 10cm) plain glass plates with 0.75mm bonded spacers for TV100
2 x (10 x 10cm) plain glass plates with 1mm bonded spacers for TV100
2 x (10 x 10cm) plain glass plates with 1.5mm bonded spacers for TV100
2 x (10 x 10cm) plain glass plates with 2mm bonded spacers for TV100
2 x (10 x 10cm) plain glass plates for TV100
2 x (10 x 10cm) notched glass plates for TV100
1 x (10 x 10 cm) dummy plate
2 x 0.75mm thick spacers for TV100
2 x 1mm thick spacers for TV100
2 x 1.5mm thick spacers for TV100
2 x 2mm thick spacers for TV100
2 x spacer aligners for TV100
1 x cam-pin casting base with 2 silicone seals
2 x silicone seals for cam-pin casting base
1 x caster system for 2 x (10 x 10cm) gradient mini-gels
1 x caster system for 10 x (10 x 10cm) gradient mini-gels
2 x silicone gaskets for “non-eared” glass plates
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power output connectors
TV100-GRM
TCS-CC
TV100-PGS0.75
TV100-PGS1
TV100-PGS1.5
TV100-PGS2
TV100-PG
TV100-NG
TV100-DP
TV100-S0.75
TV100-S1
TV100-S1.5
TV100-S2
TV100-SA
TV100-CB
TV100-CB-SEALS
TV100-MC2
TV100-MC10
TV100-SGB
PT-0.2
CABLE-4
Rat supernatant proteins separated,
following serial dilution, using the
TV100Y mini-gel electrophoresis unit
at 230V for 1 hour and 20 minutes.
Proteins were resolved in a 4%
polyacrylamide stacking gel and a
10% polyacrylamide resolving gel
made from the following SERVA
chemicals: 40% (37.5:1) acrylamide
[10681.03]; 1M Tris-HCl, pH 8.5
[39794.01]; 20% (w/v) SDS solution
[20767.03]; APS [13375.05]; TEMED
[35925.01]; 10x Laemmli buffer
[42556.01]; Tris (analytical grade)
[37180.04] and Glycine (analytical
grade) [23390.03]. Courtesy of The
University of Coventry, UK.
TV100 Combs
Part No.
*TV100-C0.75-8MC
TV100-C0.75-10
TV100-C0.75-12
*TV100-C0.75-16MC
*TV100-C1-8MC
TV100-C1-10
TV100-C1-12
*TV100-C1-16MC
*TV100-C1.5-8MC
TV100-C1.5-10
TV100-C1.5-12
*TV100-C1.5-16MC
*TV100-C2-8MC
TV100-C2-10
TV100-C2-12
*TV100-C2-16MC
*Multi-channel compatible
Thickness
(mm)
Sample
Throughput
0.75
0.75
0.75
0.75
1
1
1
1
1.5
1.5
1.5
1.5
2
2
2
2
8
10
12
16
8
10
12
16
8
10
12
16
8
10
12
16
Tooth
Width
(mm)
6
4
3.75
2.5
6
4
3.75
2.5
6
4
3.75
2.5
6
4
3.75
2.5
Tooth
Height
(mm)
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
15
Max.
Spacing
(mm)
3
3
2.25
2
3
3
2.25
2
3
3
2.25
2
3
3
2.25
2
Sample
Volume /
Well (µl)
67
45
42
28
90
60
56
37
120
90
84
55
180
120
112
75
DO YOU NEED…?
PRECAST SDS-PAGE GELS
A BLOTTING MODULE
ELECTROPHORESIS BUFFERS
RECIRCULATING CHILLER
COLLOIDAL COOMASSIE BLUE STAIN
SEE PAGE 123
SEE PAGE 60
SEE PAGE 119
SEE PAGE 82
SEE PAGE 119
51
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Complete System
Twin-plate mini-gel unit with GRM and gel tank, lid,
2 x (10 x 10cm; W x H) plain glass plates, 4 x 1mm spacers,
2 x spacer aligners, 2 x (10 x 10cm) notched glass plates,
1 x dummy plate and 2 x 1mm thick 12-sample combs
Vertical Gel Units
BENEFITS INCLUDE
• Twin-plate 20.5 x 10cm (W x H) wide
format mini-gel unit - designed
specifically for PAGE techniques
requiring self-cast gels
• Wide format - 20.5cm gel width
effectively allows 2 mini-gels to be run
within the same gel
• Glass plate stops - within the newly
modified GRM - lock the glass plates
firmly into position onto soft silicone
gaskets in the casting base to ensure
leak-free gel-casting
TV200
• Additional gel casting - with the optional
TV400-CB cam-pin casting base or
TV400-MC2 and 2 gel multicaster
• Float glass plates - 4mm thick guarantee uniform gel thickness and
even sample migration, and with ground
edges to inhibit sample leakage during
casting
• Twin screws - on each gel-clamp - act in
conjunction with a counterbalanced,
dovetailed silicone gasket to disperse
pressure evenly along the height and
breadth of the gel as the screws are
tightened
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
TV200 Standard
Twin-Plate Wide Format Mini-Gel
Electrophoresis Unit
The TV200 20.5 x 10cm (W x H) wide format mini-gel units allows
more samples to be screened on the same gel, eliminating the
problems associated with comparing samples from 2 separate
tape-cast mini-gels.
TECHNICAL SPECIFICATION
TV200
Unit Dimensions (W x D x H)
30 x 15 x 14cm
Inner Tank Dimensions (W x D x H)
27 x 11 x 11.5cm
Plate Dimensions (W x H x T)
20.5 x 10 x 0.4cm
Standard Spacer Dimensions (W x H x T)
2 x 20 x 0.1cm
Active Gel Dimensions (W x H)
16.5 x 8.5cm
Maximum Sample Capacity
2 x 48
Recommended Buffer Volume
Inner Buffer Chamber 300ml
Gel Tank
Recommended Running Conditions for
Denaturing/Native PAGE Gel
2800ml
Voltage
100 - 150V
(10 - 15V/cm)
Current
10 - 15mA
Time
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
52
1.5 - 2h
Inner Diameter
n/a
Outer Diameter
n/a
Inner Diameter
n/a
Outer Diameter
n/a
Power Output Connectors (diameter)
Shrouded, 4mm
Recommended Power Supply
Consort EV243
BENEFITS INCLUDE
• Twin-plate 20.5 x 10cm (W x H) wide
format mini-gel unit - designed
specifically for PAGE techniques requiring
self-cast gels
• Wide format - 20.5cm gel width effectively
allows 2 mini-gels to be run within the
same gel
• Casting base - included as standard
TV200Y with casting
• Additional gel casting - with the optional
TV400-MC2 and 2 gel multicasters
TV200Y Standard
Twin-Plate Wide Format
Mini-Gel Electrophoresis Unit
• Float glass plates - 4mm thick guarantee uniform gel thickness and even
sample migration, and with ground edges
to inhibit sample leakage during casting
The TV200Y twin-plate 20.5 x 10cm (W x H) wide format mini-gel
units allows more samples to be screened on the same gel,
eliminating the problems associated with comparing samples from
2 separate mini-gels. This unit includes, as standard., a precision
engineered casting base, which acts in conjunction with our
recently modified TV200-GRM to provide simple, leak-free casting.
• Twin screws - on each gel-clamp - act in
conjunction with a counterbalanced,
dovetailed silicone gasket to disperse
pressure evenly along the height and
breadth of the gel as the screws are
tightened
• Colour coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
TECHNICAL SPECIFICATION
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Plate Dimensions (W x H x T)
Standard Spacer Dimensions (W x H x T)
Active Gel Dimensions (W x H)
Maximum Sample Capacity
Recommended Buffer Volume
Recommended Running Conditions for
Denaturing/Native PAGE Gel
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Casting Base Silicone Seal
Dimensions (W x L x H)
Power Output Connectors (diameter)
Recommended Power Supplies
TV200Y
30 x 15 x 14cm
27 x 11 x 11.5cm
20.5 x 10 x 0.4cm
2 x 20 x 0.1cm
16.5 x 8.5cm
2 x 48
Inner Buffer
300ml
Chamber
Gel Tank
2800ml
Voltage
100 - 150V
(10- 15V/cm)
Current
10 - 15mA
Time
1.5 - 2h
Inner Diameter
n/a
Outer Diameter
n/a
Inner Diameter
n/a
Outer Diameter
n/a
1.7 x 22.5 x 0.8cm
Shrouded, 4mm
Consort EV243
53
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
• Glass plate stops - within the newly
modified GRM - lock the glass plates firmly
into position onto soft silicone gaskets in
the casting base to ensure leak-free gelcasting
SDS-PAGE METHOD
Vertical Gel Units
NATIVE PAGE
Native PAGE resolves proteins by their
individual charge to mass ratios.
For
successful application of this technique,
samples must be kept at 4°C and all conditions
or steps conducive to protein denaturation,
such as SDS, excessive sample agitation and
heat incubation, should be omitted from the
above protocol. Native PAGE is applied in
many areas of research: from resolving
proteins of identical molecular weight to
recovering biologically active proteins from cell
lysates.
Making polyacrylamide gels by the
tape-casting method
Plain glass plate
Notched
glass
plate
Spacer
Tape 3
sides of
the gel
cassette
SDS-PAGE allows proteins to separate on the basis of their molecular weight (MW) and is
dependent on the action of SDS - a detergent that equalises the charge to mass ratio of each
protein by coating it with a uniform negative charge - and the sieving action of the polyacrylamide
gel matrix. This technique is particularly useful for determining the MW of individual protein
subunits and as the first step of western blotting to examine the post-translational modification of
individual proteins. Scie-Plas recommends using high quality chemicals in the application of this
technique and their part numbers are shown (see pages 117-123 for further details).
1. Clean glass plates with distilled water and methanol.
2. Tape 3 sides of each gel cassette to form a leak-free seal and insert into the GRM (see diagram
below for tape-cast gels).
3. Prepare and pour a 10% resolving gel as follows for two 10 x 10cm: Resolving gel
Part No.
7.5ml 40% (37.5:1) acrylamide
(10681.01)
11.25ml 1M Tris-HCl, pH 8.5
(39794.01)
0.15ml 20% (w/v) SDS
(20767.03)
0.1ml 10% (w/v) APS
(13375.05)
0.02ml TEMED
(35925.01)
11.0ml distilled water
4. Overlay distilled water on top of the gel, allowing the gel to set for 30 minutes.
5. Remove the water and prepare and pour a 4.5% stacking gel as follows: Stacking gel
Part No.
1.15ml 40% (37.5:1) acrylamide
(10681.01)
1.25ml 1M Tris-HCl, pH 6
(39794.01; to pH 6 with HCl)
0.05ml 20% (w/v) SDS
(20767.03)
0.03ml 10% APS
(13375.05)
0.01ml TEMED
(35925.01)
7.5ml distilled water
6. Insert the comb and allow the gel to set.
7. Mix the protein samples with Tris-Glycine/SDS sample buffer (2x) buffer (Tris/HCl pH 6, 126mM,
glycerol 20%, SDS 4%, bromophenol blue 0.02%; 42527.01) and denature for 2 minutes at 98°C
and then centrifuge for 2 minutes at 14000rpm.
8. Fill the inner buffer chamber and tank with Tris-Glycine/SDS electrophoresis buffer (10x)
(0.25M Tris, 1.92M Glycine and 1% SDS; 42529.01).
9. Load the protein samples into the wells according to the well volumes in aqueous solution.
10. Run the gel according to the operating guidelines in Technical Information.
11. After electrophoresis, turn off the power supply and remove the GRM from the tank after
emptying it of buffer.
12. Remove the plates from the GRM and gently prise them open with a spacer at a corner but
NOT at the notched ears - the gel is now ready for the next application e.g. Western Transfer,
staining etc.
Dovetailed silicone gasket forms a leak-free seal
between the inner GRM and
gel tank, making it ideal for
discontinuous PAGE
Glass plate stops serve as an immoveable
barrier, locking the
glass plates firmly into
position on the casting
base
Twin screws act, as they are
tightened, with a
silicone dovetailed
gasket to apply an even
pressure across the
height and breadth of
the gel to prevent the
inner gel chamber
from leaking
Casting base re-engineered with
deeper recesses to
accommodate soft
silicone seals which sit
flush with the bottom of
each glass plate
Silicone seals - form a leak-free
seal as the glass plates are
dragged onto the casting base
54
Cam pins - turned clockwise
to drag the glass plates
firmly onto the silicone seals
within the casting base
ORDERING INFORMATION
Complete System
Part No.
Twin-plate wide format mini-gel unit with GRM and gel tank, lid,
2 x (20.5 x 10cm; W x H) plain glass plates, 4 x 1mm spacers,
2 x spacer aligners, 2 x (20.5 x 10cm) notched glass plates,
1 x dummy plate and 2 x 1mm thick 24-sample combs
TV200
Replacement Parts & Accessories
1 x TV200 gel-running module
2 x (20.5 x 10cm) plain glass plates with 0.75mm bonded spacers for TV200
2 x (20.5 x 10cm) plain glass plates with 1mm bonded spacers for TV200
2 x (20.5 x 10cm) plain glass plates with 1.5mm bonded spacers for TV200
2 x (20.5 x 10cm) plain glass plates with 2mm bonded spacers for TV200
2 x (20.5 x 10cm) plain glass plates for TV200
2 x (20.5 x 10cm) notched glass plates for TV200
1 x (20.5 x 10cm) dummy plate
2 x 0.75mm thick spacers for TV200
2 x 1mm thick spacers for TV200
2 x 1.5mm thick spacers for TV200
2 x spacer aligners for TV200
1 x cam-pin casting base with 2 silicone seals
2 x silicone seals for cam-pin casting base
1 x caster system for 2 x (20.5 x 10cm) gradient wide format mini-gels
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power output connectors
TV200-GRM
TV200-PGS0.75
TV200-PGS1
TV200-PGS1.5
TV200-PGS2
TV200-PG
TV200-NG
TV200-DP
TV200-S0.75
TV200-S1
TV200-S1.5
TV400-SA
TV400-CB
TV400-CB-SEALS
TV200-MC2
PT-0.2
CABLE-4
TV200 Combs
Part No.
TV400-C0.35-24
*TV400-C0.35-36MC
*TV400-C0.75-18MC
TV400-C0.75-24
*TV400-C0.75-36MC
TV400-C0.75-48
*TV400-C1-18MC
TV400-C1-24
*TV400-C1-36MC
TV400-C1-48
*TV400-C1.5-18MC
TV400-C1.5-24
*TV400-C1.5-36MC
TV400-C1.5-48
*Multi-channel compatible
Thickness
(mm)
Sample
Throughput
0.35
0.35
0.75
0.75
0.75
0.75
1
1
1
1
1.5
1.5
1.5
1.5
24
36
18
24
36
48
18
24
36
48
18
24
36
48
Tooth
Width
(mm)
4.75
2.5
6
4.75
2.5
2.35
6
4.75
2.5
2.35
6
4.75
2.5
2.35
Tooth
Height
(mm)
5
5
20
20
20
20
20
20
20
20
20
20
20
20
Max.
Spacing
(mm)
2
2
3
2
2
1
3
2
2
1
3
2
2
1
Sample
Volume /
Well (µl)
8
4
90
71
37
35
120
95
50
47
180
142
75
70
55
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Twin-plate wide format mini-gel unit with GRM and gel tank, lid,
2 x (20.5 x 10cm; W x H) plain glass plates, 4 x 1mm spacers,
2 x spacer aligners, 2 x (20.5 x 10cm) notched glass plates, 1 x dummy plate,
2 x 1mm thick 24-sample combs, casting base and 2 x silicone seals
TV200Y
Vertical Gel Units
BENEFITS INCLUDE
• Twin-plate 20.5 x 20cm maxi-gel unit designed specifically for higher
resolution PAGE techniques requiring
self-cast gels
• Large format - 20.5 x 20cm glass plate
dimensions are ideal for running higher
percentage polyacrylamide gels to
resolve smaller proteins of similar MW
TV400K - cooled
• Two versions available: Standard TV400 - non-cooled
Cooled TV400K - includes a cooling
coil in the bottom of the gel tank
• Enhanced cooling - in the TV400K snap-lock connectors allow the cooling
coil within TV400YK to be connected to
an external chiller unit for faster, higher
voltage separations
• Additional gel casting - with the
optional TV400-CB cam-pin casting base
or TV400-MC2 and 2 gel multicaster
• Float glass plates - 4mm thick guarantee uniform gel thickness and
even sample migration, and with ground
edges to inhibit sample leakage during
casting
• Triple screws - on each gel-clamp - act
in conjunction with a counterbalanced,
dovetailed silicone gasket to disperse
pressure evenly along the height and
breadth of the gel as the screws are
tightened
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
TV400 - non-cooled
TV400 Standard &TV400K Cooled
Twin-Plate Maxi-Gel
Electrophoresis Units
The TV400 and TV400K twin-plate 20.5 x 20cm (W x H) maxi-gel
units offer all the benefits of the TV100 and TV200 units, but in
a much larger gel size and with increased sample throughput.
A cooling coil within the TV400K gel tank allows faster
separations to be performed at high voltage without
compromising resolution.
TECHNICAL SPECIFICATION
TV400
TV400K
Unit Dimensions (W x D x H)
28.5 x 15 x 27.5cm
38 x 15 x 27.5cm
Inner Tank Dimensions (W x D x H)
27 x 12.5 x 25cm
27 x 12.5 x 25cm
Plate Dimensions (W x H x T)
20.5 x 20 x 0.4cm
20.5 x 20 x 0.4cm
Standard Spacer Dimensions (W x H x T)
2 x 20 x 0.1cm
2 x 20 x 0.1cm
Active Gel Dimensions (W x H)
16.5 x 17.5cm
16.5 x 17.5cm
Maximum Sample Capacity
2 x 48
2 x 48
Recommended Buffer Volume
Inner Buffer Chamber 700ml
Inner Buffer Chamber 700ml
Gel Tank
Gel Tank
Recommended Running Conditions for
4200ml
Voltage
Denaturing/Native PAGE Gel
175 - 225V
(8.75 - 11.25V/cm)
Current
Time
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
56
20 - 30mA
2.5 - 3.5h
4200ml
Voltage
200 - 300V
(10 - 15V/cm)
Current
Time
30 - 40mA
2 - 2.5h
Inner Diameter
n/a
Inner Diameter
10mm
Outer Diameter
n/a
Outer Diameter
12mm
Inner Diameter
n/a
Inner Diameter
10mm
Outer Diameter
n/a
Outer Diameter
12mm
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supply
Consort EV243
Consort EV243
BENEFITS INCLUDE
• Twin-plate 20.5 x 20cm maxi-gel unit designed specifically for higher resolution
PAGE techniques requiring self-cast gels
TV400Y - non-cooled + casting
• Large format - 20.5 x 20cm glass plate
dimensions are ideal for running higher
percentage polyacrylamide gels to resolve
smaller proteins of similar molecular
weight
• Casting base - included as standard with
both units
• Two versions available: Standard TV400Y - non-cooled + casting
Cooled TV400YK - cooled + casting
TV400Y Standard &TV400YK
Cooled Twin-Plate Maxi-Gel
Electrophoresis Units
The TV400Y and TV400YK twin-plate 20.5 x 20cm (W x H) maxi-gel
units offers all the benefits of the TV100 and TV200 units, but in a
much larger gel size and with increased sample throughput.
Both units include, as standard, a precision engineered casting
base, which acts in tandem with our recently modified TV400GRM to provide simple, leak-free casting.
TECHNICAL SPECIFICATION
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Plate Dimensions (W x H x T)
Standard Spacer Dimensions (W x H x T)
Active Gel Dimensions (W x H)
Maximum Sample Capacity
Recommended Buffer Volume
Recommended Running Conditions for
Denaturing/Native PAGE Gel
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Casting Base Silicone Seal
Dimensions (W x L x H)
Power Output Connectors (diameter)
Recommended Power Supply
TV400Y
28.5 x 15 x 27.5cm
27 x 12.5 x 25cm
20.5 x 20 x 0.4cm
2 x 20 x 0.1cm
16.5 x 17.5cm
2 x 48
Inner Buffer
700ml
Chamber
Gel Tank
4200ml
Voltage
175 - 225V
(8.75 - 11.25V/cm)
Current
20 - 30mA
Time
2.5 - 3.5h
Inner Diameter
n/a
Outer Diameter
n/a
Inner Diameter
n/a
Outer Diameter
n/a
1.7 x 22.5 x 0.8cm
TV400YK
38 x 15 x 27.5cm
27 x 12.5 x 25cm
20.5 x 20 x 0.4cm
2 x 20 x 0.1cm
16.5 x 17.5cm
2 x 48
Inner Buffer
700ml
Chamber
Gel Tank
4200ml
Voltage
200 - 300V
(10 - 15V/cm)
Current
30 - 40mA
Time
2 - 2.5h
Inner Diameter
10mm
Outer Diameter
12mm
Inner Diameter
10mm
Outer Diameter
12mm
1.7 x 22.5 x 0.8cm
Shrouded, 4mm
Consort EV243
Shrouded, 4mm
Consort EV243
• Glass plate stops - within the newly
modified GRM - lock the glass plates firmly
into position onto soft silicone gaskets in
the casting base to ensure leak-free gelcasting
• Enhanced cooling - in the TV400YK - snaplock connectors allow the cooling coil
within TV400YK to be connected to an
external chiller unit for faster, higher
voltage separations
• Additional gel casting - with the optional
TV400-MC2 and 2 gel multicaster
• Float glass plates - 4mm thick guarantee uniform gel thickness and even
sample migration, and with ground edges
to inhibit sample leakage during casting
• Triple screws - on each gel-clamp - act in
conjunction with a counterbalanced,
dovetailed silicone gasket to disperse
pressure evenly along the height and
breadth of the gel as the screws are
tightened
• Colour-coded combs and spacers available in 0.75, 1, 1.5 and 2mm
thicknesses
57
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
TV400YK - cooled + casting
ORDERING INFORMATION
Vertical Gel Units
Complete System
Part No.
Twin-plate wide format mini-gel unit with GRM and gel tank, lid,
2 x (20.5 x 20cm; W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners,
2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate and
2 x 1mm thick 24-sample combs
TV400
Twin-plate wide format mini-gel unit with GRM and cooled gel tank,
with built-in cooling coil and quick-fit tubing, plus lid, 2 x (20.5 x 20cm; W x H)
plain glass plates, 4 x 1mm spacers, 2 x spacer aligners,
2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate and
2 x 1mm thick 24-sample combs
TV400K
Twin-plate wide format mini-gel unit with GRM and gel tank, lid,
2 x (20.5 x 20cm; W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners
2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate,
2 x 1mm thick 24-sample combs, casting base and 2 x silicone seals
TV400Y
Twin-plate wide format mini-gel unit with GRM and cooled gel tank, with built-in cooling coil
and quick-fit tubing, plus lid, 2 x (20.5 x 20cm; W x H) plain glass plates, 4 x 1mm spacers,
2 x spacer aligners, 2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate,
2 x 1mm thick 24-sample combs, casting base and 2 x silicone seals
TV400YK
Replacement Parts & Accessories
1 x TV400 gel-running module
2 x quick-fit tubes for cooling coil
2 x (20.5 x 20cm) plain glass plates with 0.75mm bonded spacers for TV400
2 x (20.5 x 20cm) plain glass plates with 1mm bonded spacers for TV400
2 x (20.5 x 20cm) plain glass plates with 1.5mm bonded spacers for TV400
2 x (20.5 x 20cm) plain glass plates with 2mm bonded spacers for TV400
2 x (20.5 x 20cm) plain glass plates for TV400
2 x (20.5 x 20cm) notched glass plates for TV400
1 x (20.5 x 20cm) dummy plate
2 x 0.75mm thick spacers for TV400
2 x 1mm thick spacers for TV400
2 x 1.5mm thick spacers for TV400
2 x 2mm thick spacers for TV400
2 x spacer aligners for TV400
1 x cam-pin casting base with 2 silicone seals
2 x silicone seals for cam-pin casting base
1 x caster system for 2 x (20.5 x 20cm) gradient wide format mini-gels
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with shrouded 4mm power output connectors
TV400-GRM
TCS-CC
TV400-PGS0.75
TV400-PGS1
TV400-PGS1.5
TV400-PGS2
TV400-PG
TV400-NG
TV400-DP
TV400-S0.75
TV400-S1
TV400-S1.5
TV400-S2
TV400-SA
TV400-CB
TV400-CB-SEALS
TV400-MC2
PT-0.2100CM
CABLE-4
TV400 Combs
Part No.
TV400-C0.35-24
*TV400-C0.35-36MC
*TV400-C0.75-18MC
TV400-C0.75-24
*TV400-C0.75-36MC
TV400-C0.75-48
*TV400-C1-18MC
TV400-C1-24
*TV400-C1-36MC
TV400-C1-48
*TV400-C1.5-18MC
TV400-C1.5-24
*TV400-C1.5-36MC
TV400-C1.5-48
*Multi-channel compatible
DO YOU NEED…?
ACRYLAMIDE
A CAPILLARY GEL-RUNNING MODULE
A GEL DOCUMENTATION SYSTEM
A MULTICASTER
A GRADIENT MIXER
58
SEE PAGE 118
SEE PAGE 60
SEE PAGE 96
SEE PAGE 80
SEE PAGE 79
Thickness
(mm)
Sample
Throughput
0.35
0.35
0.75
0.75
0.75
0.75
1
1
1
1
1.5
1.5
1.5
1.5
24
36
18
24
36
48
18
24
36
48
18
24
36
48
Tooth
Width
(mm)
4.75
2.5
6
4.75
2.5
2.35
6
4.75
2.5
2.35
6
4.75
2.5
2.35
Tooth
Height
(mm)
5
5
20
20
20
20
20
20
20
20
20
20
20
20
Max.
Spacing
(mm)
2
2
3
2
2
1
3
2
2
1
3
2
2
1
Sample
Volume /
Well (µl)
8
4
90
71
37
35
120
95
50
47
180
142
75
70
59
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
TV Modular Systems
Vertical Gel Units
BENEFITS INCLUDE
• Comprises - one standard GRM, one
CAPGRM and one EBGRM, which are
interchangeable within the same gel
tank, allowing the user to perform PAGE,
IEF and electroblotting either
independently or as part of a fully
integrated system
• CAPGRM (capillary gel running module)
- for first dimension electrophoresis of
up to 10 ampholyte capillary gels, a
maximum 8 or 17cm in length and 1 or
4mm in internal diameter
• Twin-plate standard GRM - designed
specifically for SDS-PAGE techniques
requiring self-cast gels, although
commercially available 10 x 10cm
precast gels may be used in the
TV100-GRM
• Twin-cassette EBGRM (Electroblotting
Gel Running Module) - allows
simultaneous transfer of 2
polyacrylamide gels in colour-coded
electroblotting cassettes, while plateelectrodes only 6cm apart maximise the
intensity of the electric field
• Casting base and cooling - included as
standard - act in tandem with glass
plate stops within the GRM to lock the
plates firmly in position on the casting
base, whereas snap-lock connectors
allow the cooling coil within the tank to
be connected to an external chiller unit
for high voltage/current separations
• Colour-coded combs and spacers available in 1 and 1.5mm thicknesses
• Nitrocellulose and PVDF membranes
available plus 10 x 10cm precast gels,
ampholytes, 2-D and PAGE chemicals see page 118-124 for further details
60
TV Complete
Modular Systems
The TV complete modular systems provide a fully
integrated solution within one gel tank for both twodimensional (2-D) electrophoresis and electroblotting.
These systems are available in existing TV100 and TV400
formats and comprise individual gel-running modules
designed specifically for denaturing or native PAGE
(GRM), capillary gel isoelectric focusing (CAPGRM) and
electroblotting (EBGRM). Following 2-D electrophoresis,
the EBGRM can be used to transfer second dimension
SDS-PAGE gels for further analysis by western blotting,
providing enhanced sensitivity.
TECHNICAL SPECIFICATION
TV100YK-MODSYS
TV400YK-MODSYS
28 x 15 x 18cm
38 x 15 x 27.5cm
Inner Tank Dimensions (W x D x H)
16.5 x 11 x 15cm
27 x 12.5 x 25cm
Plate Dimensions (W x H x T)
10 x 10 x 0.2cm
20.5 x 20 x 0.4cm
Standard Spacer Dimensions (W x H x T)
1 x 10 x 0.1cm
2 x 20 x 0.1cm
Active Gel Dimensions (W x H)
8 x 8.5cm
16.5 x 17.5cm
Sample Capacity
2 x 1 / 2 x 12
2 x 1 / 2 x 24
Recommended Buffer Volume (PAGE GRM)
Recommended Running Conditions for SDS-PAGE
Capillary Dimensions
Recommended Buffer Volume (CAPGRM)
Inner Buffer Chamber
90ml
700ml
Gel Tank
1600ml
4200ml
Voltage
100 - 150V (10 - 15V/cm)
200 - 300V (10 - 15V/cm)
Current
10 - 15mA
30 - 40mA
Time
1 - 1.75h
2 - 2.5h
Inner Diameter
1mm
1mm
Outer Diameter
5mm
5mm
Length
80mm
170mm
Upper Buffer Chamber 200ml
Gel Tank
300ml
1600ml
Recommended Running Conditions for IEF of Capillary Gels
4200ml
Pre-run
Separation
End of Run
Pre-run
Separation
End of Run
Voltage
200V
400V
500V
200V
400V
500V
Time
0.5h
3h
0.5h
0.5h
6h to
18h
0.5h
Volt-hours
100Vh
1200Vh
250Vh
100Vh
2400
-7200Vh
250Vh
Distance Between Plate Electrodes in EBGRM
6cm
Electroblotting Cassette Dimensions (W x L)
11.5 x 10.5cm
20.5 x 20cm
Active Transfer Area
71cm2
289cm2
Recommended Running Conditions for Electroblotting Voltage
Current
6cm
100V
200V
75mA (0.6-0.8mA/cm2)
150mA (0.8-1.0mA/cm2)
Time
4 - 6h
6h to overnight
Snap-lock Connectors for Cooling Coil
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Quick-fit Tubing
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Casting Base Silicone Seal Dimensions (W x L x H)
1.5 x 11 x 0.8cm
1.7 x 22.5 x 0.8cm
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supplies
Consort EV215 or EV261
Consort EV215 or EV261
ORDERING INFORMATION
Complete System
Twin-plate mini-gel unit with GRM and cooled gel tank, with built-in cooling coil and
quick-fit tubing, lid, 2 x (10 x 10cm; W x H) plain glass plates, 2 x (10 x 10cm) notched glass plates,
1 x dummy plate, 4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 12-sample combs,
casting base and 2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm thick spacers,
2 x 1.5mm thick 1-D preparatory combs, 10 x blanking ports and 1 x gel extraction platform;
plus EBGRM, 2 x electroblotting cassettes and 4 x fibre pads
Part No.
TV100YK-MODSYS
Twin-plate maxi-gel unit with GRM and cooled gel tank, with built-in cooling coil and quick-fit tubing,
lid, 2 x (20.5 x 20cm; W x H) plain glass plates, 2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate,
4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 24-sample combs, casting base and
2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm thick spacers, 2 x 1.5mm thick
1-D preparatory combs, 10 x blanking ports and 1 x gel extraction platform; plus EBGRM,
2 x electroblotting cassettes and 4 x fibre pads
TV400YK-MODSYS
61
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Unit Dimensions (W x D x H)
Vertical Gel Units
BENEFITS INCLUDE
• Comprises - one standard GRM and
one EBGRM, which are interchangeable
within the same gel tank, allowing the
user either to perform electroblotting
independently or as part of a fully
integrated system
• Twin-cassette EBGRM - available in 2
sizes to allow simultaneous transfer of 2
polyacrylamide gels
TV100-EBGRM
• Plate-electrodes - only 6cm apart maximise the intensity of the electric
field, allowing rapid transfer in as little
as 1 hour
• Colour-coded electroblotting cassettes
- corresponding to the anode and
cathode - ensure that transfer is
performed in the correct orientation,
while fibre pads compress the gel
against the membrane for uniform
transfer
• Twin-plate standard GRM - designed
specifically for SDS-PAGE techniques
requiring self-cast gels, although
commercially available 10 x 10cm
precast gels may be used in the TV100GRM
• Casting base and cooling - included as
standard - act in tandem with glass
plate stops within the GRM to lock the
plates firmly in position on the casting
base, whereas snap-lock connectors
allow the cooling coil within the tank to
be connected to an external chiller unit
for high voltage/current separations
• Colour-coded combs and spacers available in 1mm thickness
• Nitrocellulose and PVDF membranes
available plus 10 x 10cm precast gels
and blotting buffers - see pages
118-124 for further details
62
TV Modular
Electroblotting
Systems
The TV modular electroblotting systems provide a complete
solution within one gel tank for PAGE and electroblotting. These
systems are available in existing TV100 and TV400 formats, and
comprise individual gel-running modules designed specifically
for PAGE (GRM) and electroblotting (EBGRM).
TV100YK-EBSYS
TV400YK-EBSYS
Unit Dimensions (W x D x H)
28 x 15 x 18cm
38 x 15 x 27.5cm
Inner Tank Dimensions (W x D x H)
16.5 x 11 x 15cm
27 x 12.5 x 25cm
Plate Dimensions (W x H x T)
10 x 10 x 0.2cm
20.5 x 20 x 0.4cm
Standard Spacer Dimensions (W x H x T)
1 x 10 x 0.1cm
2 x 20 x 0.1cm
Active Gel Dimensions (W x H)
8 x 8.5cm
16.5 x 17.5cm
Sample Capacity
2 x 12
2 x 24
Recommended Buffer Volume (PAGE GRM)
Recommended Running Conditions for SDS-PAGE
Inner Buffer Chamber
90ml
700ml
Gel Tank
1600ml
4200ml
Voltage
100 - 150V (10 - 15V/cm)
200 - 300V (10 - 15V/cm)
Current
10 - 15mA
30 - 40mA
Time
1 - 1.75h
2 - 2.5h
Distance Between Plate Electrodes in EBGRM
6cm
6cm
Electroblotting Cassette Dimensions (W x L)
11.5 x 10.5cm
20.5 x 20cm
Active Transfer Area
71cm2
289cm2
Recommended Running Conditions for Electroblotting Voltage
Current
100V
200V
75mA (0.6-0.8mA/cm2)
150mA (0.8-1.0mA/cm2)
Time
4 - 6h
6h to overnight
Snap-lock Connectors for Cooling Coil
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Quick-fit Tubing
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Casting Base Silicone Seal Dimensions (W x L x H)
1.5 x 11 x 0.8cm
1.7 x 22.5 x 0.8cm
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supplies
Consort EV215 or EV261
Consort EV215 or EV261
ORDERING INFORMATION
Complete System
Part No.
Twin-plate mini-gel unit with GRM and cooled gel tank, with built-in cooling coil and quick-fit tubing,
lid, 2 x (10 x 10cm; W x H) plain glass plates, 2 x (10 x 10cm) notched glass plates, 1 x dummy plate,
4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 12-sample combs, casting base and
2 x silicone seals; plus EBGRM, 2 x electroblotting cassettes and 4 x fibre pads
TV100YK-EBSYS
Twin-plate maxi-gel unit with GRM and cooled gel tank, with built-in cooling coil and quick-fit tubing, lid,
2 x (20.5 x 20cm; W x H) plain glass plates, 2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate,
4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 24-sample combs, casting base
and 2 x silicone seals; plus EBGRM, 2 x electroblotting cassettes and 4 x fibre pads
TV400YK-EBSYS
63
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TECHNICAL SPECIFICATION
Vertical Gel Units
BENEFITS INCLUDE
• Comprises - one standard GRM and
one CAPGRM, which are interchangeable
within the same gel tank, allowing the
user either to perform PAGE and IEF
independently or as part of a fully
integrated 2-D system
• CAPGRM - available in 2 sizes for first
dimension electrophoresis of up to 10
ampholyte capillary gels, a maximum 8
or 17cm in length and 1 or 4mm in
internal diameter
TV400-CAPGRM
TV100-CAPGRM
• Capillary gels - available in standard
1mm internal diameter for analytical
IEF, or as optional 4mm capillaries for
protein isolation
• Gel extraction platform (GEP) - for safe
and easy collection and transfer of
fragile, extruded capillary gels
• Twin-plate standard GRM - designed
specifically for SDS-PAGE techniques
requiring self-cast gels, although
commercially available 10 x 10cm
precast gels may be used in the TV100GRM
• Casting base and cooling - included as
standard - act in tandem with glass
plate stops within the GRM to lock the
plates firmly in position on the casting
base, whereas snap-lock connectors
allow the cooling coil within the tank to
be connected to an external chiller unit
for high voltage/current separations
• Colour-coded combs and spacers available in 1 and 1.5mm thicknesses,
the latter suited to 1.5mm thick capillary
gels for second dimension 2-D
electrophoresis
• Ampholytes, 2-D and PAGE chemicals
available - see pages 118-124 for
further details
64
TV Modular
2-D Systems
The TV modular 2-D systems provide a complete
solution within one gel tank for two-dimensional (2-D)
electrophoresis. These systems are available in existing
TV100 and TV400 formats and comprise individual gelrunning modules designed specifically for denaturing or
native PAGE (GRM) and isoelectric focusing (IEF) with
capillary gels (CAPGRM).
TV100YK-2DSYS
TV400YK-2DSYS
Unit Dimensions (W x D x H)
28 x 15 x 18cm
38 x 15 x 27.5cm
Inner Tank Dimensions (W x D x H)
16.5 x 11 x 15cm
27 x 12.5 x 25cm
Plate Dimensions (W x H x T)
10 x 10 x 0.2cm
20.5 x 20 x 0.4cm
Standard Spacer Dimensions (W x H x T)
1 x 10 x 0.1cm
2 x 20 x 0.1cm
Active Gel Dimensions (W x H)
8 x 8.5cm
16.5 x 17.5cm
Sample Capacity
2 x 1 / 2 x 12
2 x 1 / 2 x 24
Recommended Buffer Volume (PAGE GRM)
Recommended Running Conditions for SDS-PAGE
Capillary Dimensions
Recommended Buffer Volume (CAPGRM)
Inner Buffer Chamber
90ml
700ml
Gel Tank
1600ml
4200ml
Voltage
100 - 150V (10 - 15V/cm)
200 - 300V (10 - 15V/cm)
Current
10 - 15mA
30 - 40mA
Time
1 - 1.75h
2 - 2.5h
Inner Diameter
1mm
1mm
Outer Diameter
5mm
5mm
Length
80mm
170mm
Upper Buffer Chamber
200ml
200ml
Gel Tank
1600ml
1600ml
Recommended Running Conditions for IEF of Capillary Gels
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Pre-run
Separation
End of Run
Pre-run
Separation
End of Run
Voltage
200V
400V
500V
200V
400V
500V
Time
0.5h
3h
0.5h
0.5h
6h to 18h
0.5h
Volt-hours
100Vh
1200Vh
250Vh
100Vh
2400Vh 250Vh
-7200Vh
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Inner Diameter
10mm
10mm
Outer Diameter
12mm
12mm
Casting Base Silicone Seal Dimensions (W x L x H)
1.5 x 11 x 0.8cm
1.7 x 22.5 x 0.8cm
Power Output Connectors (diameter)
Shrouded, 4mm
Shrouded, 4mm
Recommended Power Supplies
Consort EV215 or EV261
Consort EV215 or EV261
ORDERING INFORMATION
Complete System
Twin-plate mini-gel unit with GRM and cooled gel tank, with built-in cooling coil and quick-fit tubing,
lid, 2 x (10 x 10cm; W x H) plain glass plates, 2 x (10 x 10cm) notched glass plates, 1 x dummy plate,
4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 12-sample combs, casting base and
2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm thick spacers, 2 x 1.5mm thick
1-D preparatory combs, 10 x blanking ports and 1 x gel extraction platform
Part No.
TV100YK-2DSYS
Twin-plate maxi-gel unit with GRM and cooled gel tank, with built-in cooling coil and quick-fit tubing,
lid, 2 x (20.5 x 20cm; W x H) plain glass plates, 2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate,
4 x 1mm thick spacers, 2 x spacer aligners and 2 x 1mm thick 24-sample combs, casting base and
2 x silicone seals; CAPGRM, 10 x 1mm glass capillaries, 4 x 1.5mm thick spacers, 2 x 1.5mm thick 1-D
preparatory combs, 10 x blanking ports and 1 x gel extraction platform
TV400YK-2DSYS
65
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TECHNICAL SPECIFICATION
Vertical Gel Units
TV Modular Systems Accessories
Replacement Parts & Accessories
Description
TV100YK-MODSYS
Part No.
TV400YK-MODSYS
Part No.
1 x gel-running module
2 x quick-fit tubes for cooling coil
2 x plain glass plates
2 x notched glass plates
1 x dummy plate
2 x 1mm thick spacers
1 x 1mm thick 12-sample combs
1 x 1mm thick 24-sample combs
2 x spacer aligners
1 x cam-pin casting base with 2 silicone seals
2 x silicone seals for cam-pin casting base
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with
shrouded 4mm power output connectors
TV100-GRM
TCS-CC
TV100-PG
TV100-NG
TV100-DP
TV100-S1
TV100-C1-12
TV100-SA
TV100-CB
TV100-CB-SEALS
PT-0.2
TV400-GRM
TCS-CC
TV400-PG
TV400-NG
TV400-DP
TV400-S1
TV400-C1-24
TV400-SA
TV400-CB
TV400-CB-SEALS
PT-0.2
CABLE-4
CABLE-4
2 x 1.5mm thick spacers
1 x 1.5mm thick 1-D preparatory combs
1 x capillary gel-running module
10 x 1mm glass capillaries
10 x 4mm glass capillaries,
ideal for preparatory IEF and protein purification
10 x blanking ports
1 x gel extraction platform
TV100-S1.5
TV100-C1-1D
TV100-CAPGRM
TV100-CAPROD
TV400-S1.5
TV400-C1-1D
TV400-CAPGRM
TV400-CAPROD
TV100-CAPROD4
TV100-CAPB
TV100-GEP
TV400-CAPROD4
TV100-CAPB
TV400-GEP
TV100-EBGRM
TV100-EBC
TV100-FP
TV400-EBGRM
TV400-EBC
TV400-FP
1 x electroblotting gel-running module
with built-in plate electrodes
1 x colour-coded electroblotting cassette
4 x fibre pads
Use these part numbers when ordering replacement parts for each TV100 and TV400 complete modular
MODSYS system
Use these part numbers when ordering replacement parts for each TV100 and TV400 modular 2DSYS system
Use these part numbers when ordering replacement parts for each TV100 and TV400 modular EBSYS system
See pages 117-124 for
chemicals, reagents and
buffers for SDS-PAGE,
electroblotting and 2-D
capillary gel
electrophoresis
66
See page 124 for
Scie-Plas blotting
membranes
Scie-Plas now provide the first of a series of innovative solutions
for scientists who prefer the latest advances in IPG (immobilised
pH gradient) strip technology to more traditional capillary gels as
the first step of 2-D electrophoresis. Our recently improved flatbed isoelectric focusing system (IEF-SYS) allows 1st dimension
2-D electrophoresis to be performed with multiple IPG strips
within the same tank, while 2nd dimension SDS-PAGE is carried
out in our existing TV100 and 400 Mini- and Maxi-gel formats
(TV100YK or TV400YK).
Scie-Plas offer the complete 2-D electrophoresis system with
everything you need for running reproducible 2-D gels.
IEF-SYS FOR THE 1ST
DIMENSION
A recently redesigned IEF-SYS
unit where a maximum of 30
SERVA IPG strips, each 3mm
wide and up to 24cm in length, may
be run simultaneously on a 27 x 27cm
ceramic cooling plate.
FOR THE 2ND DIMENSION
Either the TV100YK twin-plate 10 x 10cm (W x H) mini-gel unit Or
the TV400YK twin-plate 20.5 x 20cm
(W x H) maxi-gel unit
Separation of SERVA proteome markers
(39220.01) by 2-D electrophoresis using 18cm long
SERVA IPG Bluestrips, pH 3-10, on the IEF-SYS
unit in the first dimension, followed by SDS-PAGE
in the TV400Y unit in the second dimension. An
overview of 2-D electrophoresis is provided in the
Outline Protocol, while further details can be found
in our 2-D Technical. Manual available from
[email protected]
BENEFITS INCLUDE
• Glass electrode frame - sits flush with
the ceramic cooling plate for optimal
cooling efficiency
• Positive and negative electrodes made from clear acrylic clip neatly
within a glass electrode frame that
allows the voltage gradient to be finetuned along the entire length of each
IPG strip
• Glass electrode support slab - ensures
that the electrodes remain in complete
contact with the positive and negative
ends of each IPG strip during IEF
BENEFITS INCLUDE
• Casting & cooling included as standard
• New-style GRM for leak-free casting
Adaptable either for 1mm or 1.5mm thick
gels in the 2nd dimension
67
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The TV100YK and TV400YK-2DIEF-SYS Units: Dedicated 2-D
Electrophoresis Systems
Vertical Gel Units
BENEFITS INCLUDE
• Nine different program settings - each
with 9 different parameters - allow more
complex, multi-step techniques to be
programmed and stored, while voltage
ramping, automatic crossover and
recovery after power failure are included
as standard
EV232 High-Voltage FL300 Chiller Unit
Maintains cooling plate
3kV Power Supply
The Consort EV232 power
supply - 3000V, 150mA, 150W
temperature during IEF
• High voltage 3kV output - allows
desired Volt-hours to be attained faster
at maximum voltage during IEF resulting
in shorter overall run time
• 4/2MMA adaptors - compatible with the
2mm recessed outputs of the power
supply - adapt the 4mm shrouded power
connectors of the TV100YK and TV400YK
units for lower voltage 2nd dimension
SDS-PAGE
See page 92 for further details
BENEFITS INCLUDE
• Temperature range: -20 to +40°C
• Can be easily adjusted to +20°C - the
ideal temperature for running IPG strips
• Flow rate 15L/min / Maximum
pressure 350mbar
See page 82 for further details
2-D ELECTROPHORESIS: OUTLINE PROTOCOL
1. Incubate the IPG strips overnight in a rehydration tray with buffer (e.g. 8M Urea,
1% CHAPS, 13mM DTT and 0.5% SERVALYT™ 3-10, corresponding to the pH gradient of the IPG
strip), overlaid with silicone oil, containing the protein of interest.
2. Connect the ceramic cooling plate of the IEF-SYS to the external chiller preset at 20°C, before
placing the glass electrode frame directly on the cooling plate. Apply a thin layer of silicone oil
over the glass plate before laying out the IPG strips at least 3mm apart.
3. After covering the anode and cathode ends of the IPG strips with electrode wicks saturated with
deionised water, clip each electrode into its respective position within the glass electrode frame,
before overlaying the glass electrode weight.
4. Cover the IPG strips with silicone oil to protect them from heat desiccation during IEF.
5. After IEF incubate the IPG strips for 10 minutes in Equilibration Buffer 1 (50mM Tris-HCl, pH
8.8, 6M Urea, 30% glycerol, 2% SDS, 0.01% bromophenol blue and 1% (w/v) DTT) by placing the
rehydration tray on to an agitating platform. Repeat this for a further 10 minutes with
Equilibration Buffer 2 (50mM Tris-HCl, pH 8.8, 6M Urea, 30% glycerol, 2% SDS, 0.01%
bromophenol blue and 5% (w/v) iodoacetamide).
6. Dip each strip briefly in 1 x Laemmli Buffer before aligning it by its 1mm thick edge along the
top of a pre-made 10% acrylamide gel.
7. Having covered each strip with 0.5% agarose solution, perform second-dimension SDS-PAGE .
ORDERING INFORMATION
Complete System
TV100YK + IEF-SYS + EV232 + external chiller unit
Twin-plate mini-gel unit with PAGE GRM and cooled gel tank, lid, 2 x (10 x 10cm; W x H) plain glass plates,
2 x (10 x 10cm) notched glass plates, 1 x dummy plate, 4 x 1mm spacers, 4 x 1.5mm spacers,
2 x spacer aligners, 2 x 1mm thick 1-D combs, 2 x 1.5mm thick 1-D combs, casting base,
2 x silicone seals, 1 x dummy plate, 2 x quick-fit tubes, 2 x 4mm power cables and 2 x 4/2mm
power output adaptors
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate,
electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight,
2 x quick-fit tubes and 2 x 2mm power cables
Consort EV232 3000V, 150mA, 150W power supply
Recirculating Chiller
Complete System
TV400YK + IEF-SYS + EV232 + external chiller unit
Twin-plate maxi-gel unit with PAGE GRM and cooled gel tank, lid, 2 x (20.5 x 20cm; W x H) plain glass plates,
2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate, 4 x 1mm spacers, 4 x 1.5mm spacers,
2 x spacer aligners, 2 x 1mm thick 1-D combs, 2 x 1.5mm thick 1-D combs, casting base,
2 x silicone seals, 1 x dummy plate, 2 x quick-fit tubes, 2 x 4mm power cables and 2 x 4/2mm
power output adaptors
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate,
electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight,
2 x quick-fit tubes and 2 x 2mm power cables
Consort EV232 3000V, 150mA, 150W power supply
Recirculating Chiller
68
Part No.
TV100YK-2D-IEF-SYS
TV100YK
IEF-SYS
EV232
FL300
Part No.
TV400YK-2D-IEF-SYS
TV400YK
IEF-SYS
EV232
FL300
Technical Specification
TV100YK-2D-IEF-SYS / TV400YK-2D-IEF-SYS Units
Unit dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Glass Plate Dimensions (W x L x T)
Electrode Frame Dimensions (W x L x T)
Cooling Plate Dimensions (W x L x T)
Glass Electrode Weight (W x L x T )
Adjustable Electrodes Active Dimensions (W x L x T)
Recommended Temperature for Cooling Plate
during IEF with IPG strips
Power Output Connectors (diameter)
Recommended Running Conditions for IEF of
7cm IPG strips (TV100YK-2D-IEF-SYS)
IEF-SYS (1st dimension IEF)
30 x 7cm IPG strips (dimensions: 70 x 3 x 1mm/L x W x T) - TV100YK-2D-IEF-SYS
30 x 18cm IPG strips (dimensions: 180 x 3 x 1mm/L x W x T) - TV400YK-2D-IEF-SYS
46 x 41 x 11.5cm
37 x 31 x 8.5cm
27 x 27 x 0.5cm
27 x 27 x 1cm
27 x 27 x 3cm
20 x 26.5 x 1cm
10 x 265 x 13mm
20°C
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Shrouded, 2mm
Voltage Step
Voltage (V)
Time (h)
Volt-hours
Voltage Step
Voltage (V)
Time (h)
Volt-hours
Inner Diameter
Inner Diameter
Maximum Sample Capacity
TV100YK (2nd dimension PAGE)
2 x 7cm IPG strips
Recommended Running Conditions for IEF of
18cm IPG strips (TV400YK-2D-IEF-SYS)
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Plate Dimensions (W x H x T)
Spacer Dimensions (W x H x T)
Active Gel Dimensions (W x H)
Casting Base Silicone Seal Dimensions (W x L x H)
Power Output Connectors (diameter)
Recommended Buffer Volume (PAGE GRM)
Recommended Running Conditions for Denaturing PAGE
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
1
150
0.5
75
1
300
1
300
10mm
10mm
2
300
0.5
150
2
600
1
600
20 x 15 x 18cm
16.5 x 11 x 15cm
10 x 10 x 0.2cm
1 x 10 x 0.1/0.15cm
8 x 8.5cm
1.5 x 11 x 0.8cm
Shrouded, 4mm - use 4/2MMA
adaptors for EV232 power supply
Inner Buffer Chamber
90ml
Gel Tank
1600ml
Voltage
100 - 150V (10 - 15V/cm)
Current
10 - 15mA
Time
1 - 1.75h
Inner Diameter
10mm
Outer Diameter
12mm
Inner Diameter
10mm
Outer Diameter
12mm
3
4
600
1500
0.5
0.5
300
750
3
4
1500
3000
1
12.5
1500
37500
Outer Diameter
Outer Diameter
5
6 end of run
3000
330
2.5
<20
7500
5 end of run
330
<20
12mm
12mm
TV400YK (2nd dimension PAGE)
2 x 18cm IPG strips (trimmed) / 2 x 11cm
IPG strips from other commercial sources
38 x 15 x 27.5cm
27 x 12.5 x 25cm
20.5 x 20 x 0.4cm
2 x 20 x 0.1/0.15cm
16.5 x 17.5cm
1.7 x 22.5 x 0.8cm
Shrouded, 4mm - use 4/2MMA
adaptors for EV232 power supply
Inner Buffer Chamber
700ml
Gel Tank
4200ml
Voltage
200 - 300V (10 - 15V/cm)
Current
30 - 40mA
Time
2 - 2.5h
Inner Diameter
10mm
Outer Diameter
12mm
Inner Diameter
10mm
Outer Diameter
12mm
69
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Maximum Sample Capacity
Vertical Gel Units
The TVS1400 - wide
format for
33 x 41cm (W x H)
plain and notched
glass plates,
providing increased
sample capacity
BENEFITS INCLUDE
• Safety lid - although light weight and
easily detachable, the lid cannot be
removed during electrophoresis without
breaking the electrical circuit
• Ventilation grille - aids heat dispersal
during electrophoresis and can be
replaced by the optional thermostatic fan
kit (see FHS-KIT)
• High voltage power cables - oriented
asymmetrically for correct polar
orientation - with 2mm shrouded output
connectors that are universally compatible
with modern high voltage power supplies
• Safety handles - complimented by a new
streamlined and light weight design allow easy transportation around the lab
• Spring-loaded plate clamps - act in
conjunction with counterbalanced silicone
gaskets to maintain uniform gel thickness
and even distribution of pressure across
the 4mm thick float glass plates
• Aluminium back plate - serves as a heat
sink to prevent sample distortion and
'smiling'
• Lower buffer chamber - clips directly
onto the anode and can also be used to
drain buffer from the upper chamber
• Optional FHS-KIT - an adjustable,
thermostatically controlled fan kit powered
independently to maintain gel temperature
• Melinex combs and spacers - 0.25 and
0.35mm thick - precision machined for
maximum loading accuracy and optimum
band resolution
70
The TVS1400 Single-Plate
Large Format Vertical Gel
Electrophoresis Unit
The Scie-Plas single-plate large format vertical gel
electrophoresis unit is ideal for high-resolution techniques, such
as DNA sequencing, DNase and RNase footprinting, and
heteroduplex and oligonucleotide analysis, which require longer
separation distances.
TECHNICAL SPECIFICATION
TVS1400
Unit Dimensions (W x D x H)
63 x 21.5 x 50cm
Plate Dimensions (W x H x T)
33 x 41 x 0.4cm
Standard Spacer Dimensions (W x H x T)
10 x 410 x 0.35mm
Active Gel Dimensions (W x H)
29 x 38.5cm
Maximum Sample Capacity
1 x 96
Recommended Buffer Volume
Upper Buffer Chamber
1200ml
Lower Buffer Chamber
1200ml
Recommended Running Conditions for 6%
Power
45 - 55W
Denaturing PAGE Gel Using Passive
Gel Temperature
50 - 60˚C
Ventilation at 20˚C Ambient Temperature
Time
2.5 - 3h
Recommended Running Conditions for 6%
Power
75W
Denaturing PAGE Gel Using FHS-KIT at
Gel Temperature
55˚C
20˚C Ambient Temperature
Time
1.75h
Power Output Connectors (diameter)
Shrouded, 2mm
Recommended Power Supplies
Consort EV232 & EV233
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Drainage tap allows the upper
buffer chamber
to be emptied
safely and easily
Vertical Gel Units
TECHNICAL SPECIFICATION
FHS-KIT
Unit Dimensions (W x D x H)
9.6 x 14 x 8cm
Temperature Control Range
0 - 100˚C
Temperature Control Accuracy +/-2%
Fan Dimensions (W x D x H)
12 x 12 x 3.8cm
Fan Operating Speed
2700rpm
Flow Rate
>84CFM
Noise Rating
36 dBA
Optional FHS-KIT
• Comprises a fan operated by a heat sensor
temperature control unit
• Gel temperature is set by manual adjustment of
the dial on the front panel of the control unit
• Fan is screwed onto the lid of the TVS1400 in
place of the ventilation grille
• Fan is activated within 4˚C of the preset
temperature
• Separations performed in less than 2 hours at
55˚C at 75W constant power
ORDERING INFORMATION
An autoradiograph of a 0.35mm 6%
sequencing gel run in 1.75h on a TVS1400
single-plate vertical gel electrophoresis
unit, at 75W constant power with the gel
temperature maintained at 55˚C using the
FHS-KIT. Further details on operation of
the TVS1400 units can be found in our
Technical
Manual
available
from
[email protected]
Complete System
Single-plate vertical gel electrophoresis unit with integral GRM
and aluminium back plate; upper and lower buffer chambers;
lid, 2 x (33 x 41cm; W x H) plain glass plates, 2 x (33 x 41cm)
notched glass plates, 2 x 0.35mm melinex spacers and
1 x 0.35mm thick 48-sample shark's tooth melinex comb
TVS1400
Replacement Parts & Accessories
2 x (33 x 41cm) plain glass plates for TVS1400
2 x (33 x 41cm) notched glass plates for TVS1400
2 x 0.35mm thick spacers for TVS1400
TVS1400-PG
TVS1400-NG
TVS1400-S0.35
Fan sensor kit including heat sensor temperature control unit,
fan and mains power lead for TVS1400 (220-240Va.c.)
Fan sensor kit including heat sensor temperature control unit,
fan and mains power lead for TVS1400 (100-110Va.c.)
2 x 0.2mm thick, platinum electrode wire for TVS1400
2 x 1 metre power leads with shrouded 2mm power
output connectors TVS1400
Part No.
FHS-KIT
FHS-KIT-A
PT-0.2
CABLE-2
TVS1400 Combs
Part No.
Thickness
(mm)
Sample
Throughput
0.35
0.35
0.35
0.35
0.35
TVS1400-C0.35-40
*TVS1400-C0.35-60MC
ELECTROPHORESIS
CHEMICALS
SEE PAGES 117-123
DO YOU NEED…?
GEL DRYERS
VACUUM PUMP
A POWER SUPPLY
ACRYLAMIDE
72
SEE PAGE 73
SEE PAGE 75
SEE PAGES 91-92
SEE PAGE 118
40
60
Tooth
Width
(mm)
4
2
Tooth
Height
(mm)
5
5
Max.
Spacing
(mm)
3
2.5
Sample
Volume /
Well (µl)
7
3.5
24
60
96
3
2.5
2.5
10
10
9
2.8
5.6
4.5
5
4
3.5
Shark's Tooth Combs
TVS1400-SC-M0.35-48
*TVS1400-SC-M0.35-60MC
TVS1400-SC-M0.35-96
*Multi-channel compatible
Gel Drying Systems
The GD-4534 and
GD-5040 Gel Dryers
A cast-aluminium base, overlaid with a porous stainless steel sheet, evenly distributes
the heat generated by an 800-Watt heating element, which is set in increments of +/2°C to a maximum temperature of 90°C. Both the heating element and vacuum pump
are timer-controlled so that the drying time and
vacuum pump can be set in 1-minute
increments to a maximum of 5 hours, while
each unit is lightweight, despite its robust
construction, for easy transportation around
the laboratory. Each gel dryer can be either
purchased individually or as part of a
complete system with the Vacuubrand®
gel pump.
BENEFITS INCLUDE
• 2 sizes available: • GD-4534 - 45 x 34cm (W x L) format
- accommodates one TVS1400 gel or
up to fifteen 10 x 10cm mini-gels
• GD-5040 - 50 x 40cm (W x L) format
- accommodates up to twenty 10 x
10cm mini-gels
• Drying assured within 1 hour - for
sequencing gels of dimensions up to 50
x 40cm
• Accurate temperature control - in +/2°C increments to a maximum of 90°C
• Accurate timer control - of the heating
element and vacuum pump - in +/-1
minute increments to a maximum of 5
hours
TECHNICAL SPECIFICATION
• Detachable mylar sheet and porous
GD-4534
GD-5040
Unit Dimensions (W x D x H)
57 x 40 x 11cm
63 x 48 x 12cm
Active Transfer Area (W x L)
45 x 34cm
50 x 40cm
Sample Throughput
One 33 x 41cm TVS1400
sequencing gel;
fifteen 10 x 10cm mini-gels
Two 20 x 50cm TVS1000
sequencing gels;
twenty 10 x 10cm mini-gels
polyethylene sheet - allow the gel dryer
to be adapted for thin polyacrylamide
gels and thicker agarose gels
Timer Range, Vacuum
0-5h
0-5h
Timer Range, Temperature
0-5h
0-5h
Temperature Range
Ambient-90°C
Ambient-90°C
Recommended Drying Conditions
40min at 80°C for ≤1.5mm
sequencing gel
40min at 80°C for ≤1.5mm
sequencing gel
2-3 hours at 50°C
for >1.5mm agarose gel
2-3 hours at 50°C
for >1.5mm agarose gel
Internal
Internal
Dimensions of Vacuum Port
8mm
External
9mm
8mm
Pore Size of Porous Polyethylene Cover Sheet
0.75mm
0.75mm
Pore Size of Stainless Steel Sheet
1mm
1mm
Required Flow Rate of Vacuum Source
25-100L/min
25-100L/min
External
• Stainless steel screen - acts in
conjunction with a cast-aluminium base
to serve as a porous gel support,
uniformly distributing the heat and
vacuum to prevent gel cracking during
drying
• Translucent silicone rubber overlay allows the gels to be visualised during
the drying process
• Vacuubrand® MZ 2C + 2AK pump
available
9mm
For a 110VAC electrical system use
codes GD-4534-A & GD-5040-A
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The GD-4534 and GD-5040 vacuum gel dryers are ideal for drying
sequencing gels or multiple small format gels.
Gel Drying Systems
ORDERING INFORMATION
Complete System
45 x 34cm (W x L) Slab Gel Dryer System,
including stainless steel sheet, mylar sheet,
porous polyethylene sheet and clear silicone rubber overlay sheet
Part No.
GD-4534
Replacement Parts & Accessories
Stainless steel sheet for GD-4534
Mylar sheet for GD-4534
Porous polyethylene sheet for GD-4534
Clear silicone rubber overlay sheet for GD-4534
VGD-SSS
VGD-MS
VGD-PPS
VGD-SRO
Complete System
50 x 40cm (W x L) Slab Gel Dryer System,
including stainless steel sheet, mylar sheet,
porous polyethylene sheet and clear silicone rubber overlay sheet
Part No.
GD-5040
Replacement Parts & Accessories
Stainless steel sheet for GD-5040
Mylar sheet for GD-5040
Porous polyethylene sheet for GD-5040
Clear silicone rubber overlay sheet for GD-5040
GD-SSS
GD-MS
GD-PPS
GD-SRO
GD-4534 shown with Vacuubrand MZ 2C+2 AK Vacuum
Pump (can be purchased separately)
74
The Vacuubrand®
MZ 2C + 2 AK
Vacuum Pump
BENEFITS INCLUDE
• Oil-free vacuum down to ~ 9mbar
ultimate vacuum - i.e. the lowest
pressure that can be produced and
measured reproducibly in a vacuum
system at room temperature
The Vacuubrand® MZ 2C + 2 AK is a quiet, low maintenance, oilfree diaphragm pump that supplies the low vacuum necessary
for fast gel drying with the GD-4534 and GD-5040 units.
Gas ballast valve
• Selected chemically resistant material
• Gas ballast valve as standard for
working with condensable vapours
• High pumping speed sustained at
nearly ultimate vacuum
• Solvent recovery close to 100%
• Long lifetime, easy-to-change
diaphragms and valves
• Quiet, low noise
Handle
• Efficient and environmentally friendly
Outlet
TECHNICAL SPECIFICATION
Round bottomed flask - as
catchpot at inlet
MZ 2C + 2 AK
Unit Dimensions (W x L x H) 24.1 x 34.5 x 32.6cm
Vacuum Pump
Chemistry
Diaphragm Pump,
MZ 2C
Number of Stages
2
Pumping Speed 50/60 Hz
1.9m3/h / 2.1 m3/h // 1.2cfm
Ultimate Vacuum (total)
9 mbar // 6.8 Torr
Ultimate Vacuum
with Gas Ballast
15 mbar // 11 Torr
Round bottomed flask - as
catchpot at outlet
MZ 2C + 2 AK
The chemistry vacuum system MZ 2C + 2 AK is equipped with an inlet and outlet
separator. The inlet separator prevents droplets and particles from entering the pump.
The outlet separator allows a controlled collection of condensed solvent vapours and
prevents condensate in the exhaust line from draining back into the pump.
Inlet Connection (IN)
Hose nozzle NW 10mm
Outlet Connection (EX)
Hose nozzle NW 10mm
Weight
11.9kg
Pump Flow Rate
35L/min
ORDERING INFORMATION
Complete System
Chemistry diaphragm pump MZ 2C mounted on pump support.
Separator at inlet and outlet. On/off switch, cable with plug
and instructions for use.
Part No.
68 80 18
Replacement Parts & Accessories
Hose (rubber) NW 10mm
68 60 02
For a 110VAC electrical system use
code 68 80 18-A
75
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• Compact design
Inlet
Gradient Gel Electrophoresis
BENEFITS INCLUDE
• Maximum 96-sample throughput using two 48-sample combs - allows
samples to be transferred quickly and
easily to the each gel from standard 96well microplates or thermal cycler
blocks
• Large format - 20.5 x 20cm glass plates
allow gradient gels to be poured
containing wider ranges of denaturant
concentration, maximising sensitivity
and resolution
• 100ml gradient mixer - used with the
new-style TV400-GRM and casting base
to make two 1mm parallel denaturing
gradient gels
• Formamide, urea and
acrylamide available - to
pour denaturing gradient
gels - contact [email protected]
• Can also be adapted for constant
denaturing gel electrophoresis (CDGE)
- if the denaturant concentration
required for partial melting of the DNA
target sequence is already known
The TV400-DGGE
Denaturing Gradient
Gel Electrophoresis System
The TV400-DGGE denaturing gradient gel electrophoresis system
is a cost-effective solution for researchers studying mutations
and DNA polymorphisms critical in disease aetiology and genetic
diversity. Designed primarily for parallel denaturing gradient gel
electrophoresis (DGGE), where electrophoresis and the
denaturing gradient run in the same direction, the TV400-DGGE
has a maximum 96-sample throughput compatible with standard
microplates and thermal cycler blocks. A 100ml gradient mixer
is also included to pour gradient gels using our newly designed
gel-running module and cam-caster, while a 400W heater,
regulated by an external temperature control unit connected to a
heat sensor within the gel tank, allows the gel temperature to be
set to the predetermined melting temperature (Tm) of the PCR®amplified DNA polymorphism or mutation of interest.
• 400W heating element - manually
controlled by a temperature
control unit connected to a PT100
heat sensor - enables the gel and
buffer temperature to be set in 1°C
increments to a predetermined Tm,
to a maximum 70°C
76
PT100
Heat
Sensor
400W
heating
element
Temperature Range Thermometer
Power Output of Heating Element
Heating Element Dimensions (L x D)
Operating Range of PT100 Temperature Sensor
PT100 Temperature Sensor Dimensions (L x D)
Casting Base Silicone Seal Dimensions (W x L x H)
Power Output Connectors (diameter)
Recommended Power Supplies
TV400 GRM + Heated Tank
28.5 x 15 x 29cm
27 x 12.5 x 26cm
20.5 x 20 x 0.4cm
2 x 20 x 0.1cm
16.5 x 17.5cm
2 x 24
Inner Buffer Chamber
700ml
Gel Tank
4200ml
Voltage
175 - 225V
(8.75 - 11.25V/cm)
Current
20 - 30mA
Time
2.5 - 3.5h
0 - 100˚C
400W
14 x 1.5cm
-50 to +200˚C
14 x 0.3cm
1.7 x 22.5 x 0.8cm
Shrouded, 4mm
Consort EV243
Unit Dimensions (W x D x H)
Recommended Temperature Control Range
Temperature Control Accuracy
HS-UNIT
9.6 x 14 x 8cm
50 to 70˚C
+/-1˚C
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Plate Dimensions (W x H x T)
Standard Spacer Dimensions (W x H x T)
Active Gel Dimensions (W x H)
Sample Capacity
Recommended Buffer Volume
Recommended Running Conditions for
Denaturing/Native PAGE Gel
Unit Dimensions (W x D x H)
Internal Dimensions of Mixing and
Reservoir Chambers (Diameter x Height)
Volume of Each Chamber
Internal Diameter of Outlet Port
GM100
11 x 3 x 13.5cm
PARALLEL DGGE - OVERVIEW
Parallel DGGE is based on the application of a
denaturant concentration gradient - increasing
in the direction of electrophoresis within a
polyacrylamide gel - and uniform temperature to
induce the partial unwinding or melting of
double-stranded DNA by distinct domains in a
sequence-specific manner. Melting breaks the
hydrogen bonds holding together each base pair
within a DNA sequence: with domains rich in GC base pairs melting at higher temperatures
than those with high A-T base-pair content.
Incorporating sequence high in G-C content into
the DNA target sequence by PCR® amplification
(GC-clamping) prevents the DNA target with the
lowest melting domain from melting completely
at its predetermined Tm, which is maintained by
the application of a constant temperature across
the gel and within the buffer. Because both gel
temperature and increasing denaturant
concentration act to melt DNA in a sequenceand domain-specific manner, any point
mutations amplified within a PCR® product will
affect its Tm so that its subsequent mobility
within a polyacrylamide gel differs from its wild
type counterpart (see schematic diagram).
Consequently, the sensitivity of this technique in
distinguishing individual DNA sequences, which
might differ only by a single point mutation, has
proven highly attractive to scientists screening
for unknown mutations.
Schematic of Parallel DGGE
2.5 x 12.5cm
50ml
2mm
Constant t°C
HS-UNIT heat
sensor control
unit - manually
adjustable in 1°C
increments to
the desired Tm
[Denaturant] /
electrophoresis
Low
Wild
type
Mutant
Wild type
+ Mutant
High
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TECHNICAL SPECIFICATION
Gradient Gel Electrophoresis
ORDERING INFORMATION
Complete System
Twin-plate maxi-gel unit with GRM, heated gel tank with
PT100 temperature sensor and thermometer, lid,
2 x (20.5 x 20cm; W x H) plain glass plates,
2 x (20.5 x 20cm) notched glass plates, 4 x 1mm spacers,
2 x spacer aligners and 2 x 1mm thick 24-sample combs,
casting base, 2 x silicone seals and 1 x dummy plate;
plus GM100 gradient mixer and heat sensor control unit
Part No.
TV400-DGGE
as above for 110VAC
TV400-DGGE-A
Replacement Parts & Accessories
1 x TV400 gel-running module
2 x (20.5 x 20cm) plain glass plates for TV400
2 x (20.5 x 20cm) notched glass plates for TV400
1 x dummy plate
2 x 1mm thick spacers for TV400
2 x spacer aligners for TV400
1 x cam-pin casting base with 2 silicone seals
2 x silicone seals for cam-pin casting base
1 x 100ml gradient mixer
1 x caster system for 2 x (20.5 x 20cm) gradient maxi-gels
2 x 0.2mm thick, platinum electrode wire
TV400-GRM
TV400-PG
TV400-NG
TV400-DP
TV400-S1
TV400-SA
TV400-CB
TV400-CB-SEALS
GM100
TV400-MC2
PT-0.2
2 x 1 metre power leads with shrouded 4mm power output connectors CABLE-4
Reagents & Chemicals
1 x Formamide, 100ml
1 x Urea, 1kg
1 x Acrylamide-Bis Solution, 37.5:1 (40% w/v), 2.6%C, 500ml
21710.01
24524.02
10681.01
TV400-DGGE Combs
Part No.
DO YOU NEED…?
A GRADIENT MIXER
ACRYLAMIDE
A GEL MULTICASTER
A POWER SUPPLY
A GEL DOCUMENTATION SYSTEM
78
SEE PAGE 79
SEE PAGE 118
SEE PAGE 80
SEE PAGES 91-92
SEE PAGE 96
TV400-C1-24 (standard)
TV400-C1-48 (optional)
Thickness
(mm)
Sample
Throughput
1
1
24
48
Tooth
Width
(mm)
4.75
2.35
Tooth
Height
(mm)
20
20
Max.
Spacing
(mm)
2
1
Sample
Volume /
Well (µl)
95
47
BENEFITS INCLUDE
• Available in 5 different sizes - for
different applications
• GM15 and GM25 - 15 and 25ml
volumes for sucrose and caesium
chloride gradients; the GM25 can
also make two 1mm thick, 10 x 10cm
gradient gels in the TV100-MC2 2-gel
multicaster
Available in 15, 25, 50, 100 and 500ml volumes, Scie-Plas's
gradient mixers are ideal for pouring polyacrylamide gradient
gels and centrifugation gradients using sucrose or caesium
chloride solutions. Each gradient mixer is precision engineered
from high quality acrylic plastic and comprises leak-free valves
that control
mixing between
the reservoir and
Mixing chamber
mixing
chambers and
Reservoir chamber
the resultant
Outlet valve
output flow.
Support rod
Mixing
valve
• GM50 - 50ml volume sufficient to
pour up to five 1mm thick, 10 x 10cm
gradient gels or one standard 1mm
thick, 20.5 x 20cm gel using the
TV400 casting base
• GM100 - 100ml volume for pouring
up to ten 1mm thick, 10 x 10cm gels
in the TV100-MC10 10-gel
multicaster or two 1mm thick, 20.5 x
20cm gels using the TV400-MC2 2gel multicaster
• GM500 - 500ml volume suitable for
up to ten 1mm thick, 20.5 x 20cm
gels
• Reservoir chamber - designed to
contain higher percentage acrylamide
solution when making gradient gels with
the TV100 and TV400 multicasters
• Mixing chamber - each is of sufficient
TECHNICAL SPECIFICATION
GM100
GM500
diameter to accommodate a magnetic
stirrer to mix lower percentage
acrylamide with higher percentage
acrylamide from the reservoir chamber
GM15
GM25
GM50
Unit Dimensions (W x D x H)
9 x 2.4 x 9cm
10 x 2.4 x 11.5cm
10 x 2.4 x 11.5cm 11 x 3 x 13.5cm 13 x 6 x 14cm
Internal Dimensions of Mixing
and Reservoir Chambers
(Diameter x Height)
1.3 x 8cm
1.4 x 10cm
1.9 x 10cm
2.5 x 12.5cm
5.4 x 13cm
Volume of Each Chamber
7.5ml
12.5ml
25ml
50ml
250ml
• Flat base - allows each gradient mixer
Internal Diameter of Outlet Port
2mm
2mm
2mm
2mm
5mm
to be placed onto a magnetic stirring
plate
• Valves - control output flow from both
ORDERING INFORMATION
Complete System
1 x 15ml gradient mixer
1 x 25ml gradient mixer
1 x 50ml gradient mixer
1 x 100ml gradient mixer
1 x 500ml gradient mixer
the reservoir and mixing chambers
• Support rod - secures each gradient
Part No.
GM15
GM25
GM50
GM100
GM500
mixer to a retort stand
• Can be used in conjunction with the
Buffer Recirculation Pump see page 35
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Gradient Mixers
Gradient Gel Electrophoresis
BENEFITS INCLUDE
• Available in 4 different formats: • TV100-MC2 - casts two 1mm thick,
10 x 10cm standard or gradient
mini-gels
• TV100-MC10 - casts up to ten 1mm
thick, 10 x 10cm standard or
gradient mini-gels
• TV200-MC2 - casts two 1mm thick,
20.5 x 10cm standard or gradient
wide format mini-gels
• TV400-MC2 - casts two 1mm thick,
20.5 x 20cm standard or gradient
maxi-gels
• Dovetailed silicone gasket - acts in
conjunction with 5 nylon screws to
distribute pressure evenly across the
glass plates for leak-free casting
TV100, TV200 and TV400
Gel Multicasters
Scie-Plas now offers 2 and 10 gel multicasters for use with its 10
x 10cm (W x H) TV100 mini-gel, 20.5 x 10cm TV200 wide format
mini-gel and 20.5 x 20cm TV400 maxi-gel units. Gels can be
poured either as gradient gels with the hose connected to the
inlet port at the bottom of the front plate, or as standard
polyacrylamide gels from the top of the casting chamber using a
pipette.
• Separation sheets - 1mm thick - for
easy separation of each gel cassette
after casting and to maintain the correct
pressure between the glass plates and
the front plate of the casting chamber,
ensuring that straight gels, of uniform
thickness, are poured
Casting chamber
Front plate
• V-shaped cavity - stabilises the gradient
V-shaped cavity
in the bottom of casting chamber
• Inlet ports - maintain the steady flow of
acrylamide into the casting unit before
polymerisation
Hose
80
Nylon
screws
Inlet
port
Separation Sheets
TV100-MC2
TV100-MC10
TV200-MC2
TV400-MC2
Unit Dimensions (W x D x H)
13 x 3.2 x 13.5cm
13 x 8 x 13.5cm
23.5 x 4 x 11.5cm
23.5 x 4 x 21.5cm
Casting Chamber Dimensions (W x D x H)
10 x 1.2 x 10cm
10 x 6 x 10cm
20.5 x 2 x 10cm
20.5 x 2 x 20cm
Internal Diameter of Outlet Port
2mm
2mm
2mm
2mm
Compatible gradient mixer
GM25/GM50
GM100
GM50/100
GM100
ORDERING INFORMATION
Complete System
1 x 2-gel multicaster for 10 x 10cm mini-gels includes 10 x 1mm
separation sheets, 2 x 0.4mm spacer aligners and 3mm internal
diameter nylon hose
1 x 10-gel multicaster for 10 x 10cm mini-gels includes 20 x 1mm
separation sheets, 2 x 0.4mm spacer aligners and 3mm internal
diameter nylon hose
Replacement Parts & Accessories
10 x 1mm separation sheets for TV100-MC2 and TV100-MC10
2 x spacer aligners for TV100
Part No.
TV100-MC2
TV100-MC10
TV100-SS
TV100-SA
Complete System
Part No.
1 x 2-gel multicaster for 20.5 x 10cm wide format mini-gels includes
10 x 1mm separation sheets, 2 x 0.4mm spacer aligners and
3mm internal diameter nylon hose
TV200-MC2
Replacement Parts & Accessories
10 x 1mm separation sheets for TV200-MC2
2 x spacer aligners for TV200
Complete System
1 x 2-gel multicaster for 20.5 x 20cm maxi-gels includes 10 x 1mm
separation sheets, 2 x 0.4mm spacer aligners and 3mm internal
diameter nylon hose
Replacement Parts & Accessories
10 x 1mm separation sheets for TV400-MC2
2 x spacer aligners for TV400
TV200-SS
TV200-SA
Part No.
TV400-MC2
TV400-SS
TV400-SA
For glass plates and
spacers compatible
with the…
• TV100-MC2 and
TV100-MC10 see page 51
• TV200-MC2
see page 55
• TV400-MC2
see page 58
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TECHNICAL SPECIFICATION
Gel Temperature Control
BENEFITS INCLUDE
Recirculating Cooler/Chiller
• Ergonomic design and easy operation
• Splash-proof keypad with integrated
mains switch
• Large, bright LED
• Reliable microprocessor PID
temperature control
• Filling level indicator
• Powerful immersion pumps - suitable
for continuous operation
• Maximum permissible temperature of
+80°C - in the return line from the unit
to the chiller
• Easy access to filling chamber - via a
hinged lid at the top of the unit
• Low liquid level protection with visible
and audible alarm signal
• Integrated stainless steel bath tanks
• Removable venting grid for cleaning of
the condenser
• Front drain
• No side vents - allow multiple units to
be stacked alongside each other
• RS232 interface for PC connection
• IP class according to IEC 529: IP21
TECHNICAL SPECIFICATION
Working Temperature Range
-20 to +40°C
Temperature Control
PID
Temperature Stability
±0.5°C
Display
LED
Display Resolution
0.1°C
Integrated Programmer
Not available
Heater Capacity
0W
Cooling Capacity
20
300
10
250
0
200
-5
-20 °C
150 100 W
Refrigerant
R134a
Pump Capacity
Pressure: 350mbar
Flow rate: 15L/min
Digital Interfaces
RS232
Filling Volume
3 to 4.5L
Suitable Fluids
Water,
water-glycerol &
JULABO thermal
bath fluids
Fittings
Barbed fittings for
tubing of 8 and
12mm internal
diameter
Pump Connections
M16x1 male
Dimensions (W x L x H)
25 x 50 x 60cm
Weight
41kg
Ambient Temperature
5 to 40°C
Classification according to
DIN 12876-1
-
Available mains power
82
230V/50Hz;
115V/60Hz;
230V/60Hz
The recommended recirculating
cooler/chiller option for all Scie-Plas
horizontal and vertical cooled gel
electrophoresis units. Unlike other
chiller units, which are factory
preset at 4°C, the working
temperature of the FL300 can be set
anywhere between -20 and +40°C,
making it ideally suited to many
techniques ranging from standard
cooled gel electrophoresis to IEF
with IPG strips. PID (Proportional
Band, Integral, Derivative) control
guarantees thermal stability within
±0.5°C of the set temperature,
obviating the need for continuous
operator monitoring. Each unit can be easily cleaned and is
supplied with a splash-proof keypad with a bright, easy-to-read
LED that indicates the current temperature, while an RS232
interface is located at the front of each unit for PC connection and
alarm shutdown. A lift-up cover on the top of the unit allows
access to the filling port, while another hinged tray serves as
storage for the operating manual and installation documents. A
removable venting grid provides ready access to the condenser
and drainage tap for cleaning. All models include an easily visible
level indicator for the coolant, whereas vents positioned at the
front and rear allow multiple units to be placed side by side, saving
space within the laboratory.
ORDERING INFORMATION
Complete System
FL300 recirculating cooler/chiller unit for 230 Vac
mains power supply
(please specify if 230V/50Hz or 230V/60Hz is required)
FL300 recirculating cooler/chiller unit for 110 Vac
mains power supply
Part No.
FL300
FL300-A
83
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Blotting Units
Blotting Units
BENEFITS INCLUDE
• Available in 2 different formats: • TV100-EBK - accommodates a
maximum of four 10 x 10cm
electroblotting cassettes
• TV400-EBK - accommodates a
maximum of four 20.5 x 20cm
electroblotting cassettes
• Asymmetric, colour-coded cassettes hinged, with red and black covers
corresponding to the anode and the
cathode, to ensure correct transfer
• Fibre pads - compress the gel against
the nitrocellulose membrane for uniform
transfer
• Plate-electrodes - 12.8cm apart maximise the intensity of the electric
field, allowing rapid transfer within 4 to 6
hours
• Enhanced cooling - included as standard
- snap-lock connectors allow the cooling
coil within each unit to be connected to
an external chiller units for faster, highintensity transfers, or extended overnight
runs without buffer depletion
• Flat bottom - allows each electroblotter
to be placed directly onto a magnetic
stirring plate
• Blotting paper, membranes and
chemicals also available see pages
117-124
The TV100-EBK Mini and
TV400-EBK Maxi Electroblotters
Recently redesigned to accommodate up to 4 electroblotting
cassettes, the Scie-Plas TV100-EBK and TV400-EBK
electroblotters provide a cost-effective and safe and easy to use
system for the transfer of proteins and nucleic acids from 10 x
10cm mini and 20.5 x 20cm maxi gels. Both units are supplied
with asymmetric colour-coded cassettes as a unique design
feature to prevent them from being inserted into the tank in the
wrong orientation, against the direction of transfer.
Snap-lock
connectors
for enhanced
cooling
84
Asymmetric
colour-coded
cassettes
Fibre pads maintain uniform
contact between the gel and the
membrane
ELECTROBLOTTING CASSETTE ASSEMBLY
Compression cassette
Compression cassette
Foam pads
Foam pads
3MM Whatman paper
3MM Whatman paper
Polyacrylamide gel
PVDF / Nitrocellulose
membrane
Direction of transfer
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Maximum Sample Capacity
Recommended Buffer Volume
Recommended Running Conditions
for Electroblotting
Distance Between
Plate Electrodes in EBGRM
Electroblotting Cassette
Dimensions (W x L)
Active Transfer Area
Snap-lock Connectors for Cooling Coil
Quick-fit Tubing
Power Output Connectors (diameter)
Recommended Power Supply
TV100-EBK
20 x 22.5 x 18.5cm
12.8 x 11 x 17cm
4 cassettes
2000ml
Voltage
50 - 100V
Current 75mA (0.6-0.8mA/cm2)
Time
2-4h
TV400-EBK
19.5 x 32.5 x 28.5cm
12.8 x 21 x 26cm
4 cassettes
6300ml
Voltage
200V
Current 150mA (0.8-1.0mA/cm2)
Time
6h to overnight
12.8cm
12.8cm
11.5 x 10.5cm
71cm2
Inner Diameter
Outer Diameter
Inner Diameter
Outer Diameter
Shrouded, 4mm
Consort EV243
20.5 x 20cm
289cm2
Inner Diameter
Outer Diameter
Inner Diameter
Outer Diameter
Shrouded, 4mm
Consort EV243
10mm
12mm
10mm
12mm
10mm
12mm
10mm
12mm
ELECTROPHORESIS
CHEMICALS
SEE PAGES 117-123
ORDERING INFORMATION
Complete System
Complete electroblotting system for four 10 x 10cm mini-gels,
including 4 x electroblotting cassettes, 4 x fibre pads, cooled tank,
lid and quick-fit tubing
Complete electroblotting system for four 20.5 x 20cm maxi-gels,
including 4 x electroblotting cassettes, 4 x fibre pads, cooled tank,
lid and quick-fit tubing
Replacement Parts & Accessories
1 x (10 x 10cm) hinged, colour-coded cassette with fibre pads
4 x (10 x 10cm) fibre pads
1 x (20.5 x 20cm) hinged, colour-coded cassette with fibre pads
4 x (20.5 x 20cm) fibre pads
2 x quick-fit tubes for cooling coil
2 x 0.2mm thick, platinum electrode wire
2 x 1 metre power leads with
shrouded 4mm power output connectors
Part No.
TV100-EBK
TV400-EBK
TV100-EBC
EB-FPS
TV400-EBC
EB-FPL
TCS-CC
PT-0.2100CM
DO YOU NEED…?
BLOTTING PAPER & MEMBRANES
TRANSFER BUFFER
A RECIRCULATING CHILLER
A POWER SUPPLY
SEE PAGE 124
SEE PAGE 124
SEE PAGE 82
SEE PAGES 91-92
CABLE-4
85
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TECHNICAL SPECIFICATION
Blotting Units
BENEFITS INCLUDE
• 2 sizes available: • V10-SDB – 10 x 10cm format accommodates one 10 x 10cm
mini-gel
• V20-SDB – 20 x 20cm format accommodates up to four 10 x
10cm mini-gels or one 20 x
20cm maxi-gel
• Gels can be stacked – for higher
throughput blotting
• Rapid transfer assured within 1 hour –
for lower molecular weight proteins and
nucleic acids; larger molecules
transferred in 2 hours
• Semi-dry format – minimises buffer
consumption, with no need for mess and
additional accessories
• Colour-coded, corrosion-free plate
electrodes – platinum-coated titanium
anode and stainless steel cathode
maximise the transfer area, so that full
electrical contact is made between the
gel and the membrane, while corrosionresistant metal prolongs lifespan and
durability
• Blotting paper, membranes and
Stainless steel
cathode
Gel
Membrane
Blotting paper
Platinumcoated
titanium anode
Direction of Transfer
chemicals also available – see page 124
Semi-dry blotting with Scie-Plas
V10-SDB and V20-SDB units
Three sheets of blotting paper, saturated
with transfer buffer, are first superimposed
over the anode plate, followed by the
membrane, the gel and a further 3 sheets
of blotting paper. The cathode plate is then
placed over the blotting sandwich and the
current applied at 0.8mAcm-2 for 1 to 2
hours.
86
The V10-SDB and V20-SDB
Semi-Dry Blotters
The V10-SDB 10 x 10cm and V20-SDB 20 x 20cm semi-dry
blotters are ideal for fast transfer of proteins and nucleic acids
without the need for costly accessories such as gel cassettes and
tanks. Corrosion-free platinum-coated titanium and stainless
steel plate electrodes, corresponding to the anode and cathode,
maximise the active area of transfer so that full electrical contact
is made between the gel and the membrane, allowing transfer to
be completed within an hour with the minimum of heat
dissipation, buffer consumption and mess in the laboratory,
normally associated with wet blotting techniques.
TECHNICAL SPECIFICATION
Unit Dimensions (W x D x H)
Active Transfer Area (W x L)
Sample Throughput
V10-SDB
17 x 7.5 x 17cm
10 x 10cm
One 10 x 10cm mini-gel
Recommended Current Setting
Power Output Connectors (diameter)
Recommended Power Supplies
80mA / 0.8mAcm-2
4mm
EV243
V20-SDB
27 x 7.5 x 27cm
20 x 20cm
Four 10 x 10cm mini-gels;
One 20 x 20cm maxi-gel
320mA / 0.8mAcm-2
4mm
EV265, 261 & 215
ORDERING INFORMATION
Complete System
10 x 10cm semi-dry blotter
20 x 20cm semi-dry blotter
Part No.
V10-SDB
V20-SDB
The CP-2826 Maxi
Capillary Blotter
Floating lid maintains even pressure
across the gel without the need to apply
extra weight.
The gel-membrane sandwich is
overlaid with additional materials in
the following order:
1. blotting paper,
2. absorbent towel
and 3. cardboard.
BENEFITS INCLUDE
• CP-2826 – 28 x 26cm wick - for
simultaneous transfer of seven
10 x 10cm gels
• Permanent wick – serves as porous
support for the gel, eliminating the
problems associated with uneven
transfer by allowing buffer to be drawn
more efficiently from the lower tank,
thus effecting nucleic acid transfer in
<3 hours
• Simple design – guarantees fast and
Gel-membrane
sandwich.
The porous polyethylene wick serves as a gel support, which draws
transfer buffer from the lower chamber by capillary action.
Lower buffer
chamber
containing
transfer buffer.
Schematic Diagram of the Maxi Capillary Blotter
Once impregnated with buffer the porous polyethylene wick draws buffer through the gel
by capillary action, evenly transferring nucleic acids onto the membrane regardless of
their molecular weight. Any surrounding area of the wick not involved in transfer is
masked with cellophane wrapping to prevent uneven transfer.
easy set up
• Floating lid – compresses the gel
against the membrane for uniform
transfer without the need to apply a
weight, causing mechanical damage to
the gel
• Blotting paper, membranes and
chemicals also available see pages
117-124
TECHNICAL SPECIFICATION
CP-2826
41.5 x 29 x 10.5cm
38.5 x 27 x 5cm
728cm2
500ml
Unit Dimensions (W x D x H)
Inner Tank Dimensions (W x D x H)
Active Transfer Area
Recommended Buffer Volume
Transfer Time for DNA/RNA
from a 6mm, 0.8 to 1.2% Agarose Gel
2h
ORDERING INFORMATION
Complete System
Capillary blotting unit (28 x 26cm; W x L)
Part No.
CP-2826
87
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The CP-2826 maxi capillary blotter is ideal for the simultaneous
Northern and Southern transfer of multiple gels. A novel design
feature of a permanent wick set within the lower tank is optimised
to draw up more buffer than traditional systems, eliminating the
need for messy overhanging paper wicks and maximising transfer
efficiency to less than 3 hours for nucleic acids
Blotting Units
BENEFITS INCLUDE
• Rapid transfer assured within minutes
- dot/slot microfiltration manifold allows
unfractionated samples to be
immobilised onto a membrane for
immediate screening with nucleic acid
probes or antibodies
• Available in 3 different formats: • DHM-48 - for 5 x 12.8cm
membranes - 48-sample throughput
• DHM-96 - for 7.5 x 11.3cm
membranes - 96-sample throughput
• SHM-48 - for 5 x 12.8cm
membranes - 48-sample throughput
• Compatibility (DHM Only) - with multichannel pipettes and standard 96-well
microtitre plates ensures fast loading of
the manifold
• Simple design - based on the alignment
of stainless steel thumbscrews and
mating screws - guarantees fast and
easy set up without the need for gaskets
and 'O' rings
• Dot/slot blot imaging and quantitation available on the Vision gel
documentation system
• Blotting paper, membranes and
chemicals also available see pages
117-124
DO YOU NEED…?
BLOTTING PAPER & MEMBRANES
A VACUUM PUMP
A THERMAL CYCLER
A UV STERILISATION CABINET
88
SEE PAGE 124
SEE PAGE 75
SEE PAGE 99
SEE PAGE 98
Dot and Slot Blot
Microfiltration Manifolds
Available in alphanumeric 48 and 96 sample throughputs,
Scie-Plas's dot and slot blot microfiltration manifolds provide an
easy and reproducible means to hybridise proteins and nucleic
acids in solution onto membranes. These manifolds, which are
machined from high-density acrylic, have precision-lapped
mating surfaces that ensure uniform filter/membrane contact
without gaskets and 'O' rings. Manifold assembly is simple,
being achieved by the alignment and tightening of stainless steel
thumbscrews and mating screws, while filters can be cut down to
the exact size of the template. Standard manifold configurations
are available with the DHM-48 and -96 units providing 48 and 96
dots, 3mm in diameter, that are compatible with the
configuration of 96-well microtitre plates. The SHM-48 units
focus up to 48 samples into thin lines, less than 0.5 x 6mm in
size, making densitometric quantitation more reproducible.
Each hybridisation manifold requires a vacuum pump or water
aspirator equivalent to 600mm Hg (0.8 Bar).
Upper manifold - allows sample
to be loaded into each dot/slot
Template allows
membranes
to be cut
down to size
Vacuum Port can be
connected to a
water aspirator
or vacuum
pump
Vacuum reservoir - serves as a
collection chamber for filtrate
TECHNICAL SPECIFICATION
Unit Dimensions (W x D x H)
Maximum Sample Capacity
Dot Diameter
Slot Dimensions (W x L)
Sample Volume / Dot
Sample Volume / Slot
Minimum Membrane Size (W x L)
Vacuum Port External Diameter
Vacuum Port Internal Diameter
Maximum Operating Temperature
Working Pressure of Pump
or Water Aspirator
SHM-48
18.5 x 7.5 x 7cm
48
3 x 12mm
300µl
5 x 12.8cm
7mm
6mm
65°C
600mm Hg
(0.8 Bar)
DHM-48
18.5 x 7.5 x 7cm
48
3mm
100µl
5 x 12.8cm
7mm
6mm
65°C
600mm Hg
(0.8 Bar)
DHM-96
17 x 10.5 x 7cm
96
3mm
100µl
7.5 x 11.3cm
7mm
6mm
65°C
600mm Hg
(0.8 Bar)
ORDERING INFORMATION
Complete System
48-well Dot Blot Hybridisation Manifold in 3 x 16 array,
comprising paired upper dot-forming modules for dots
3mm in diameter, and lower vacuum chamber module.
96-well Dot Blot Hybridisation Manifold in 8 x 12 array,
comprising paired upper dot-forming modules for dots
3mm in diameter, and lower vacuum chamber module.
48-well Slot Blot Hybridisation Manifold in 3 x 16 array,
comprising paired upper slot-forming module for slots
0.5 x 6mm in size, and lower vacuum chamber module.
Part No.
DHM-48
DHM-96
SHM-48
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Stainless steel
thumbscrews and
precision-lapped
mating surfaces maintain vacuum
transfer without
sample smearing,
cross contamination
and leakage
Power Supplies
Power Supply Accessories
• CABLE-4 - power output connectors,
4mm in diameter, designed for low-tomedium voltage power supplies.
Supplied with all Scie-Plas horizontal
and vertical gel electrophoresis units
except the IEF-SYS.
• CABLE-2 - power output connectors,
2mm in diameter, designed for high
voltage power supplies. Only supplied
with the IEF-SYS and TVS1400 units.
Although the power cables supplied with all standard Scie-Plas
horizontal and vertical gel electrophoresis units have 4mm
output connectors that are compatible with most modern low-tomedium voltage power supplies, problems with compatibility do
arise occasionally when the power supply in the laboratory is
from a different manufacturer. Scie-Plas has three solutions
available to address this: • 4/4MMA adaptors - allow standard Scie-Plas horizontal and vertical gel
electrophoresis units to be connected to competitor low-to-medium voltage
power supplies with deeply recessed power output terminals (i.e. banana
jacks), 4mm in diameter
• 4/2MMA adaptors - allow standard Scie-Plas horizontal and vertical gel
electrophoresis units to be connected to high voltage power supplies and
those with deeply recessed power output terminals (i.e. banana jacks),
2mm in diameter
• 2/4MMA adaptors - allow the 2mm output connectors on Scie-Plas's
specialised high-voltage electrophoresis units, such as the IEF-SYS and
TVS1400, to be connected to low voltage powers supplies with 4mm outputs
for low voltage applications
ORDERING INFORMATION
Accessory
2 x 4mm-to-4mm adaptors for power supplies
with 4mm output terminals
2 x 4mm-to-2mm adaptors for power supplies
with 2mm output terminals
2 x 2mm-to-4mm adaptors for power supplies
with 4mm output terminals
2 x 1 metre power leads with 2mm output connectors
2 x 1 metre power leads with 4mm output connectors
90
Part No.
4/4MMA
4/2MMA
2/4MMA
CABLE-2
CABLE-4
Consort EV200 Range Power
Supply Units
BENEFITS INCLUDE
The Consort EV200 power
supplies cover the range of
voltages and currents used
with all Scie-Plas
electrophoresis units,
whether it is for routine low
voltage horizontal and
vertical gel electrophoresis
or high voltage applications
such as isoelectric focusing
and sequencing.
• Compact, portable and safe
• Manual and method programming
• Constant voltage, current or power
settings
• Automatic crossover
• Overload protection
• Short circuit protection
• Three-year warranty
Also introducing…
The NEW Consort EV222
Power Supply
An ideal low cost power supply for routine
horizontal and vertical gel electrophoresis
which includes many features of the Consort
EV200 units: manual programming, timer,
automatic crossover, recovery after power
failure and a 3-year warranty.
ORDERING INFORMATION
Power Supply
200 Volt, 200 Milliamp, 20 Watt power supply for 230Vac mains supply
300 Volt, 1000 Milliamp, 150 Watt power supply for 230Vac mains supply
300 Volt, 2000 Milliamp, 300 Watt power supply for 230Vac mains supply
400 Volt, 300 Milliamp, 50 Watt power supply for 230Vac mains supply
600 Volt, 500 Milliamp, 150 Watt power supply for 230Vac mains supply
600 Volt, 1000 Milliamp, 300 Watt power supply for 230Vac mains supply
1200 Volt, 500 Milliamp, 300 Watt power supply for 230Vac mains supply
3000 Volt, 150 Milliamp, 150 Watt power supply for 230Vac mains supply
3000 Volt, 300 Milliamp, 300 Watt power supply for 230Vac mains supply
6000 Volt, 150 Milliamp, 300 Watt power supply for 230Vac mains supply
For 110VAC electricity systems add -A to the end of these codes
Part No.
EV222
EV231
EV202
EV243
EV265
EV261
EV215
EV232
EV233
EV262
TECHNICAL SPECIFICATION
EV222
Voltage
0...200V
Current
0...200mA
Power
0...20W
Parameter Range
1...100%
of full scale
Timer
0...99:59h
Volt-hours
n/a
Display
LCD,
2x16 characters
Resolution
1V, 1mA, 1W
Programs
1 set of
parameters
Outputs
3 in parallel,
4mm sockets
Minimum load resistance
15Ω
Buffer depletion detection
n/a
No load detection
on/off,
programmable
Ground leakage detection
Overload detection
Computer control
n/a
Data/Logging
n/a
RS232
n/a
Ambient Temperature
0...40°C
Relative Humidity
0...95%,
non condensing
Power Requirements
210...250VAC,
50/60Hz, 35W
Dimensions (WxDxH)
24x20x13cm
Weight
2kg
91
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The voltage and current can be adjusted at any time during a run, and are displayed on
the unit in bright, easy-to-read digits. Nine different program settings, each with 9
different parameters, allow more complex, multi-step techniques to be programmed
and stored. All Consort EV200 power supplies include voltage ramping, automatic
crossover and recovery after power failure as standard, in addition to full protection
against overload and accidental short circuit.
Power Supplies
TECHNICAL SPECIFICATION
VOLTAGE
CURRENT
POWER
PARAMETER RANGE
TIMER
VOLT-HOURS
DISPLAY
RESOLUTION
PROGRAMS
OUTPUTS
MINIMUM LOAD RESISTANCE
BUFFER DEPLETION DETECTION
NO LOAD DETECTION
GROUND LEAKAGE DETECTION
OVERLOAD DETECTION
COMPUTER CONTROL
DATA/LOGGING
RS232
AMBIENT TEMPERATURE
RELATIVE HUMIDITY
POWER REQUIREMENTS
DIMENSIONS (WxDxH)
WEIGHT
VOLTAGE
CURRENT
POWER
PARAMETER RANGE
TIMER
VOLT-HOURS
DISPLAY
RESOLUTION
PROGRAMS
OUTPUTS
MINIMUM LOAD RESISTANCE
BUFFER DEPLETION DETECTION
NO LOAD DETECTION
GROUND LEAKAGE DETECTION
OVERLOAD DETECTION
COMPUTER CONTROL
DATA/LOGGING
RS232
AMBIENT TEMPERATURE
RELATIVE HUMIDITY
POWER REQUIREMENTS
DIMENSIONS (WxDxH)
WEIGHT
VOLTAGE
CURRENT
POWER
PARAMETER RANGE
TIMER
VOLT-HOURS
DISPLAY
RESOLUTION
PROGRAMS
OUTPUTS
MINIMUM LOAD RESISTANCE
BUFFER DEPLETION DETECTION
NO LOAD DETECTION
GROUND LEAKAGE DETECTION
OVERLOAD DETECTION
COMPUTER CONTROL
DATA/LOGGING
RS232
AMBIENT TEMPERATURE
RELATIVE HUMIDITY
POWER REQUIREMENTS
DIMENSIONS (WxDxH)
WEIGHT
92
EV243
0...400V
0...300mA
0...50W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
3 in parallel, 4mm sockets
30Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 75W
24x20x13cm
3kg
EV202
0...300V
0...2000mA
0...300W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 4mm sockets
5Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 360W
31x26x15cm
10kg
EV232
0...3000V
0...150mA
0...150W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 2mm sockets
600Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 200W
31x26x15cm
6kg
EV231
0...300V
0...1000mA
0...150W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 4mm sockets
10Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 200W
31x26x15cm
6kg
EV261
0...600V
0...1000mA
0...300W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 4mm sockets
15Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 360W
31x26x15cm
10kg
EV233
0...3000V
0...300mA
0...300W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 2mm sockets
300Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 360W
31x26x15cm
10kg
EV265
0...600V
0...500mA
0...150W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 4mm sockets
30Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 200W
31x26x15cm
6kg
EV215
0...1200V
0...500mA
0...300W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 4mm sockets
70Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 360W
31x26x15cm
10kg
EV262
0...6000V
0...150mA
0...300W
1...100% of full scale
0...99:59h
0...99.99kVh
LCD, 2x16 characters
1V, 1mA, 1W
9x9 set of parameters
4 in parallel, 2mm sockets
1200Ω
on/off, programmable
on/off, programmable
3600 values
9600b/s
0...40°C
0...95%, non condensing
210...250VAC, 50/60Hz, 360W
31x26x15cm
10kg
Electrodialysis
BENEFITS INCLUDE
• Fast dialysis times - due to rapid
movement of charged molecules in an
electric field
The ElectroPrep™
• Re-usable
• Different chambers - for large or small
The ElectroPrep system from Harvard Apparatus is an extremely
versatile patented sample preparation technology based on the
principles of electrophoresis and dialysis. This ElectroPrep™
system is ideal for the rapid purification of proteins, nucleic
acids, carbohydrates and other biological molecules.
With a run-time of 5 to 10 minutes, ElectroPrep™ provides speed
and convenience, even at the very low 5 to 10mA currents used
with this system. The sample chambers are made of Teflon, a
completely inert material especially suited to high sample
recovery. Membranes with molecular weight cut off points
(MWCOs), ranging from 100 to 300,000 Daltons, may be used in
combination with different Dialysis and Link Chambers for
selective elution, filtration, dialysis, fractionation and
concentration of complex samples, while Union chambers enable
different Dialysis Chambers to be joined together to increase
sample volume.
sample volumes
• Membranes available - with MWCOs to
suit almost any application or molecule
size
• Easy to use
• Leak proof
• Autoclavable
• Inert Teflon construction - minimises
non-specific protein and nucleic acid
binding to the sample chamber,
enhancing sample recovery
• Highly versatile - can be adapted for
multiple dialysis applications
Applications
• Electroelution from gels and solutions e.g. gel extraction of vector and insert
during cloning
• Electrodialysis (with an average buffer
exchange time of 5 to 10 minutes)
• On-line electrodialysis
• Electroconcentration
• Selective electrofiltration
• Size-fractionation of DNA and proteins
from complex lysates
• Primer removal following PCR®
amplification
Teflon Caps - lock
membranes into
position, and, in their
simplest format, are
used to concentrate or
desalt sample as
shown in (iv) over the
page.
Link Chamber - available in
25, 50, 100, 250 and 500µl
volumes - can be connected
to a Dialysis Chamber of
greater volume to
concentrate dialysed
product, or can be joined in
series with other link
chambers and membranes
of different MWCOs for the
size-fractionation of
different dialysed products
Membranes - with
different MWCOs to
accommodate
biological molecules
ranging from 100 to
300,000Da
Dialysis Chamber the major receptacle
for sample or
dialysed product
• Salt removal from DNA mini-preps
• Detergent removal
• Dye-Terminator removal
TECHNICAL SPECIFICATION
Unit Dimensions (W x L x H)
ORDERING INFORMATION
Unit Only
ElectroPrep™ Tank, including 4mm shrouded power connectors
Replacement Parts & Accessories
2 x 1-metre power leads with
shrouded 4 mm power output connectors
12.5 x 25 x 14cm
Internal Dimensions (W x L x H) 9.5 x 20 x 8cm
Part No.
EP-74-1101
CABLE-4
See Tables 1a, 1b, 2a & 2b to order Dialysis Chambers, Link Chambers, Unions
and Membranes
Buffer Volume
1500ml
Recommended
Running Conditions
5 to 10mA; 10
minutes run-time
Power Output
Connectors (diameter)
Shrouded, 4mm
Recommended Power Supplies Consort EV243
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
™
Electrodialysis
–
+
–
+
How to select your chamber and
membrane configurations?
c
1. Decide upon your application - e.g.
electroelution, electrodialysis,
electrofiltration or electroconcentration
2. Select a Dialysis Chamber able to
accommodate the desired sample
volume - e.g. 50, 100, 200, 500, 1000 or
1500µl - N.B. a Dialysis Chamber can
be joined to a Union to increase the
sample volume
3. Choose membranes with the
appropriate MWCO, depending on the
technique being performed (see i to iv)
and the molecular weight of the
biological molecule of interest (dialysed
product), taking into account each
membrane's suitability for use in
aqueous solutions or organic solvents
• For organic solvents, use either
regenerated cellulose or
polycarbonate
• For aqueous solutions, use cellulose
acetate
b
b
a
1
a
1
2
2
(i) Rapid and Selective
Key
Electro-Filtration
/
Concentration
Teflon Chamber
The sample is placed in a
Teflon Cap
compartment comprising the
Dialysis Chamber (1) and the
Membrane
Union.
The MWCO of
Union
membrane (b) is larger than
the molecular weight of the
biological molecule of interest,
while the MWCO of membrane (a) is smaller. Upon
application of the electric field, the biological molecule
of interest will pass through membrane (b) and collect
in Dialysis Chamber (2) while smaller molecules will
pass through membranes (a) and (b) and Dialysis
Chamber (2) as a result. This is a fast and effective
method for the concentration of small samples as well
as selective filtration.
–
+
Key
(ii) Gel extraction of DNA and
protein by Electroelution
Teflon Chamber
By using the ElectroPrep™
system as shown in the
Teflon Cap
schematic diagram, proteins and
DNA can be eluted from a gel
Membrane
slice quickly and easily with
Union
excellent recovery. By using a
Union, Dialysis Chambers can be
Gel
joined in any combination
necessary to accommodate the
size of the gel slice. Samples can be concentrated if
desired, by choosing a receiving chamber with a smaller
volume. The MWCO of the membranes (a and b) can also
be chosen to achieve very selective filtration. Membrane
(b) should have a MWCO greater than the molecular
weight of the sample of interest, while membranes (a)
and (c) are smaller. The same principle applies as
described in (i).
–
+
c
b
b
b
a
1
a
2
4. Connect the Dialysis Chamber via a
membrane and Union to another
Dialysis Chamber for electrofiltration (i)
or electroelution (ii); a Dialysis
Chamber of smaller volume can also
be attached to concentrate the dialysed
product (iii)
5. When desalting PCR® products,
membranes can be secured at each
end of the Dialysis Chamber by Teflon
end caps (iv)
6. Link chambers can be attached to the
Dialysis Chamber via a membrane to
filter samples according to their size;
multiple link chambers can be used
with different membranes for sizefractionation of different dialysed
products
(iii)
Selective
ElectroKey
Filtration / Concentration /
Teflon Chamber
Separation Based on Different
Charges
of
Biological
Teflon Cap
Molecules
In this configuration of the
Membrane
ElectroPrep™, the sample is
Union
placed in a Union between two
membranes (b), both of which
should have a MWCO larger than the molecular weight
of the molecule of interest, while the MWCOs of
membranes (a) and (c) are smaller. Each biological
molecule, including the molecule of interest, will then
move either to Dialysis Chamber (1) or Dialysis
Chamber (2), depending on its respective charge
polarity (i.e. positive or negative), whereas those
biological molecules with molecular weights lower
than the sample of interest will migrate through
membranes (a) and (c) into the tank. Biological
molecules with unknown isoelectric points can also be
separated and purified in this manner. Dialysis
Chambers of smaller volumes can be used to
concentrate dialysed product.
(iv) Electrodialysis through
Key
Simultaneous Exchange of
Teflon Chamber
Buffers - e.g. cleaning up PCR®
products
Teflon Cap
Following amplification, the
PCR® product is placed in the
Membrane
Dialysis Chamber between
membranes (a) and (b), each
membrane having a MWCO lower than the molecular
weight of the PCR® product but not the primer. Upon
application of the electric field, dialysis occurs quickly
by the simultaneous exchange of buffer, resulting in
the migration of the lower molecular weight primers
through membranes (a) and (b) while the purified PCR®
product is retained in the Dialysis Chamber.
Electrodialysis is also effective for desalting neutral
molecules that do not move in an electric field (such as
sugars) or charged molecules at their isoelectric point.
Membranes
Scie-Plas provides multiple membranes designed for use with different Dialysis Chambers, Link Chambers and Unions.
Cellulose Acetate
These membranes are low-protein-binding and have a sharp MWCO range. The membranes are pre-cut and supplied in 0.05% sodium azide
solution. They are ready to use after rinsing with deionised water and buffer. Glycerol, sulphur, and heavy metals are not present in these
membranes. Cellulose acetate membranes are intended only for aqueous solutions, and the presence of an organic solvent is not recommended.
Regenerated Cellulose
These membranes are more stable in organic solvents, but the MWCO range is not as sharply defined as that of cellulose acetate membranes.
Regenerated Cellulose membranes are pre-cut, and supplied in a 0.05% sodium azide solution. They are ready to use after rinsing with deionised
water and buffer. Glycerol, sulphur, or heavy metals are not present in these membranes.
Polycarbonate
These membranes are more stable in organic solvents. They are available in four highly controlled pore sizes for a well-defined MWCO range.
94
1a. Direct Compatibility Table to Connect Dialysis Chambers (50-200µl) and Link Chambers (25-100µl)
Dialysis Chamber
Quantity
Union(s)
Volume
Part No.
1
50µl
EP-74-0408
5
50µl
EP-74-0400
1
100µl
EP-74-0409
5
100µl
EP-74-0401
1
200µl
EP-74-0410
5
200µl
EP-74-0402
Quantity
1
Volume
Part No.
25-200µl
EP-74-0100
Link Chamber(s)
See Option.
Quantity
Volume
Part No.
A
1
25µl
EP-74-1619
to 25-200µl
OR
1
25-200µl
EP-74-0102
B
to 250-1500µl
5
25µl
EP-74-1620
1
50µl
EP-74-1611
5
50µl
EP-74-1615
1
100µl
EP-74-1612
5
100µl
EP-74-1616
Option A: Use Union EP-74-0100 to connect together any two of the 50-200µl Dialysis Chambers (EP-74-0408 to EP-74-0402).
All 50-200µl Dialysis Chambers (EP-74-0408 to EP-74-0402) are directly compatible with all 25-100µl Link Chambers (EP-74-1619 to EP-74-1616).
Option B: Union EP-74-0102 is used to connect one 50-200µl Dialysis Chamber (EP-74-0408 to EP-74-0402) with one 500-1500µl Dialysis Chamber (EP-74-0411 to EP-74-0405).
All 500-1500µl Dialysis Chambers (EP-74-0411 to EP-74-0405) are directly compatible with all 250-500µl Link Chambers (EP-74-1613 to EP-74-1618).
MWCO (kDa)
Cellulose Acetate
Part No.
Regenerated Cellulose
Part No.
Polycarbonate
Part No.
25/pack
25/pack
25/pack
0.1
EP 7424-CA100
0.5
EP 7424-CA500
1
EP 7424-CA1K
EP 7427-RC1K
2
EP 7424-CA2K
EP 7427-RC2K
5
EP 7424-CA5K
EP 7427-RC5K
10
EP 7424-CA10K
EP 7427-RC10K
25
EP 7424-CA25K
EP 7427-RC25K
50
EP 7424-CA50K
EP 7427-RC50K
100
EP 7424-CA100K
300
EP 7424-CA300K
Membrane Pore Sizes
0.01µM
EP 7431-PC01
0.05µM
EP 7431-PC05
0.10µM
EP 7431-PC10
0.6µM
EP 7431-PC60
2a. Direct Compatibility Table to Connect Dialysis Chambers (500-1500µl) and Link Chambers (250-500µl)
Dialysis Chamber
Quantity
Union(s)
Link Chamber(s)
Volume
Part No.
Quantity
Volume
Part No.
See Option.
Quantity
Volume
Part No.
500 µl
EP-74-0411
1
250-1500µl to
EP-74-1105
A
1
250µl
EP-74-1613
5
500µl
EP-74-0403
5
250µl
EP-74-1617
1
1000µl
EP-74-0412
5
1000µl
EP-74-0404
1
1
1500µl
EP-74-0413
5
1500µl
EP-74-0405
250-1500µl
OR
OR
1
25-200µl to
EP-74-0102
B
250-1500µl
1
500µl
EP-74-1614
5
500µl
EP-74-1618
Option A: Use Union EP-74-1105 to connect together any two of the 500-1500µl Dialysis Chambers (EP-74-0411 to EP-74-0405).
All 500-1500µl Dialysis Chambers (EP-74-0411 to EP-74-0405) are directly compatible with all 250-500µl Link Chambers (EP-74-1613 to EP-74-1618).
Option B: Union EP-74-0102 is used to connect one 50-200µl Dialysis Chamber (EP-74-0408 to EP-74-0402) with one 500-1500µl Dialysis Chamber (EP-74-0411 to EP-74-0405).
All 500-1500µl Dialysis Chambers (EP-74-0411 to EP-74-0405) are directly compatible with all 250-500µl Link Chambers (EP-74-1613 to EP-74-1618).
2b. Membranes Compatible with 250-1500µl Dialysis Chambers and Link 250-500µl Chambers
MWCO (kDa)
Cellulose Acetate
Part No.
25/pack
Regenerated Cellulose
Part No.
25/pack
0.1
EP 7425-CA100
0.5
EP 7425-CA500
1
EP 7425-CA1K
EP 7428-RC1K
2
EP 7425-CA2K
EP 7428-RC2K
5
EP 7425-CA5K
EP 7428-RC5K
10
EP 7425-CA10K
EP 7428-RC10K
25
EP 7425-CA25K
EP 7428-RC25K
50
EP 7425-CA50K
EP 7428-RC50K
100
EP 7425-CA100K
300
EP 7425-CA300K
Polycarbonate
Part No.
25/pack
Membrane Pore Sizes
0.01µM
EP 7432-PC01
0.05µM
EP 7432-PC05
0.10µM
EP 7432-PC10
0.60µM
EP 7432-PC60
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
1b. Membranes Compatible with 50-200µl Dialysis Chambers and 25-100µl Link Chambers
Gel Documentation
Scie-Plas VISION Gel
Documentation System
The Scie-Plas VISION is a new compact imaging system for gel
documentation, ideally suited to scientists who do not want
another PC in their laboratory. Based on the latest image
storage technology, VISION allows gel images to be printed
instantly or saved on a USB memory stick for archiving or further
processing. Also available is a network capability using either a
WiFi or an ethernet connection. This easy to upgrade system
comes complete with powerful GeneTools analysis software.
Using VISION is simple. The darkroom is designed to accommodate the most commonly
available 20 x 20cm bench top transilluminators used to illuminate gels. Using a white
light or blue light converter, VISION can be adapted to view and capture images as
follows: -
BENEFITS INCLUDE
• A self-explanatory simple to use
system - requiring no specialist on-site
installation or training
• Agar plates of dark, light or two-colour colonies
• Cells in flasks
• DNA, RNA or protein on membranes
• Spot and slot blots of DNA, RNA or protein
• Cells or solutions in microtitre plates
• DNA or protein macroarrays
• Built in USB Drive - allows rapid image
saving and upgrading of software
TECHNICAL SPECIFICATION
• High resolution images - recorded in
real time on an integrated colour LCD
screen, using an 8-bit 768 x 582 pixel
camera
• Filter drawer with optional
interchangeable filters - capable of
viewing a range of different fluorescent
stains
• UV-to-white light converter screen, 302
x 330mm - for easy imaging of protein
gels, autoradiographs and colony plates
• Darkroom - an ideal money-saving
feature, universally compatible with all
brands of standard mini bench top
transilluminators
• Maximum gel size - 20 x 20cm - makes
Vision the perfect gel documentation
system for all standard Scie-Plas
horizontal and vertical gel
electrophoresis units, as well as most
alternative brands
• Save images in TIFF or BMP format via a USB connection either to a memory
stick, network or WIFI
• GeneTools Analysis Software - saves
time by automating analysis of gels,
colony plates and blots
• 3-year warranty - for complete peace of
mind
96
Camera
8-bit mono, 768 x 562 Pixels
CCD
1/2 inch
Zoom
8 - 48 manual zoom
Lens
F1.2
Exposure Control
0.04 - 10 seconds
Detection Sensitivity
0.01ng
Image Storage
USB
Image Format
BMP or TIFF
Transilluminator
Filter size
Wavelength
Variable intensity
Compatible Dyes
Ethidium Bromide, Coomassie Blue, Silver Stain, SYBR® Gold,
SYBR® Green, SYBR® Safe, GelStar®, Sypro® Red, Sypro® Ruby,
Sypro® Orange, Fluorescein, Rhodamine Red™, Texas Red™, Pro Q®
Diamond, Deep Purple™
20 x 20cm
302nm
50-100%
Deep Purple™ is a registered trademark of Amersham Biosciences Ltd. Pro Q®, SYBR®, Sypro®, Sypro®, Rhodamine
Red™ and Texas Red™ are trademarks of Molecular Probes Inc. GelStar® is a trademark of FMC Corporation.
ORDERING INFORMATION
Complete System
Part No.
Vision gel documentation system, including transilluminator, VISION
white light converter and GeneTools software for
230 Vac mains power supply
Vision gel documentation system, including transilluminator, VISION-A
white light converter and GeneTools software for
110 Vac mains power supply
Replacement Parts & Accessories
1 x transilluninator, 302nm, 20 x 20cm
1 x UV-to-white light converter, 302 x 330mm
GVM20-E
CONVERTWHITE-2030
BENEFITS INCLUDE
• Compatible with multiple media
formats - horizontal and vertical gels;
MADGE gels, films, ELISA and TLC
plates; and dot and slot blots
molecular weight/base pair; Rf and band
quantity determination; colony counting;
densitometry; spot blot and band
matching
GeneTools Software
• Software automation - ensures rapid
image generation and data acquisition
GeneTools is an advanced, but easy to operate, 1-D gel and 2-D
spot blot analysis software that can be purchased either as part
of the Scie-Plas Vision gel documentation system or separately
for use with other gel documentation systems. An extensive and
flexible array of analysis options enables GeneTools to handle a
range of media, including gels, TLC plates, films and blots, while
its many automated features allow gel images to be loaded,
processed and quantified rapidly in as little as a few seconds.
• Automatic band matching and analysis
of multi-layered gels - simultaneous
analysis of multiple well lines per gel
• Automatic correction of background
and band and dot distortion
• Automatic/manual marker assignment
• Integral molecular weight calibration
library
• Full GLP reporting - e.g. gel, lane,
molecular weight, quantity and track
histogram
• Multiple data display options - curves,
bar charts, pie charts etc.
ORDERING INFORMATION
Complete System
GeneTools analysis software
• Results - may be saved in Excel or *.CSV
Part No.
GENETOOLS
(comma-separated value) format for
export
97
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
• Other applications comprise -
PCR® Products
BENEFITS INCLUDE
• Four 15-Watt UV-C bulbs - generate
sufficient high energy UV-C irradiation to
denature nucleic acids in as little as 30
minutes, minimising unwanted
background contamination in PCR
reactions
• Optimal reflectors - provide uniform
irradiation of the whole work surface
area within the cabinet
• Timer-control - affords the user total
control over exposure time and UVdosage
• Timer override switch - for constant
UV-C irradiation
• Safety interlocks - immediately cut out
the UV source when the side doors are
opened, preventing accidental UV
exposure
• 10mm optical acrylic - acts as a safety
barrier to UV-C and β radiation, allowing
the cabinet to be used as a radiation
work station if preferred
• White light - illuminates the work area
when the cabinet is in use
• Recommended for use
with the RPP-TY6854
safety tray and RPP-TL6854 easy-clean
tray liners (not included)
• Techne® thermal cyclers also available
TECHNICAL SPECIFICATION
External Dimensions (W x D x H) 56 x 42 x 77cm
Internal Dimensions (W x D x H) 54 x 40 x 75cm
Thickness of Acrylic
10mm; confers
protection
against the UV-C
wavelength and
high energy
β-emitters
UV-C Bulbs
4 x 15W;
254nm wavelength
White Light Bulb
1 x 15W;
visible wavelength
Timer Control
0-120 minutes
Weight
19Kg
Universal Voltage Input
100-240VAC,
50/60Hz
98
The GLE-UVSC PCR® UV
Sterilisation Cabinet
The Scie-Plas GLE-UVSC PCR® UV sterilisation cabinet provides
effective decontamination of reagents and equipment before
carrying out sensitive PCR® reactions, particularly when
amplifying DNA sequences which are either in limited supply or
of low copy number. Four timer-controlled 15-Watt UV-C bulbs
enable the user to control the exposure time and dose of high
energy UV-C irradiation required to denature nucleic acids that
cause unwanted background contamination, while a timer
override switch allows the UV source to remain on indefinitely if
preferred. Safety interlocks switch-off the UV-C bulbs
automatically when the cabinet side doors are opened, preventing
accidental exposure to the UV source. The cabinet's construction
from UV-C-impermeable 10mm acrylic also serves as an
effective barrier against radioactive isotopes, allowing the user to
work in complete safety with high energy β-emitters such as 32P.
A single white light bulb illuminates the work area when the
cabinet is in use.
PCR is a registered trademark of Hoffman LaRoche Ltd.
ORDERING INFORMATION
Complete System
UV sterilisation cabinet with universal power supply
Part No.
GLE-UVSC
Replacement Parts & Accessories
1 x UV germicidal bulb, 15W, 254nm
1 x PVC radiation safety tray, 68 x 54cm (W x L)
25 x APET liners, 68 x 54cm (W x L)
34-0008-01
RPP-TY6854
RPP-TL6854
Techne® TC-3000
Thermal Cycler
The TC-3000 is the latest upgrade of the Techne® TC-312: one of
the best selling, most reliable and inexpensive personal thermal
cyclers available on today's market.
Designed specifically to meet the budgets and PCR® applications of research and
teaching laboratories, the TC-3000 offers all the benefits of the TC-312 to ensure
reproducible high performance, including rapid heating and temperature uniformity of
the heating block; a robust, spring-loaded heated lid; PC control and fastprogramming with free software upgrades. In addition to the TC-3000, Scie-Plas can
also offer many other products from the Techne® range, including the TC-412 and TC512 thermal cyclers, hybridisation incubators, Dri-Block® heaters, water baths, sample
concentrators and biological stirrers. Contact [email protected] for a full Techne®
product catalogue and quotation.
BENEFITS INCLUDE
• Small, low cost - of the world's best
selling thermal cyclers, the TC-3000 is
the smallest, most cost-effective unit on
the market
TECHNICAL SPECIFICATION
• Available in 25 x 0.2ml or 20 x 0.5ml
Block Temperature
Number of Programs
99 (3 segment
programs)
No of 0.2ml Wells
25
No of 0.5ml Wells
20
Alphanumeric Key Pad
Yes
Colour of Wells
Blue/Black
Program Naming Capability
Alphanumeric
Block Temperature Range
4ºC to 99ºC
Heating Ramp Rate*
3.6ºC/sec
minimum
Program Password Protection Yes
Display
Large 4-line
alphanumeric
Maximum Number of
Segments/Loops
80
Cooling Ramp Rate 0.2 ml block* 2.0ºC/sec
minimum
Maximum Number of
Repeat Cycles per Program
99
Cooling Ramp Rate 0.5 ml block* 1.8ºC/sec
minimum
Uniformity at 50 ºC
0.2ºC
End of Program Alarm
Yes (can be disabled)
Uniformity at 72 ºC
0.4ºC
Pause Facility
Yes
Uniformity at 95 C
1.0ºC
Incremental/Decremental
Timing & Temperature
Yes
Accuracy at 50 ºC
± 0.2ºC
Accuracy at 72 ºC
± 0.4ºC
Accuracy at 95 ºC
± 0.3ºC
Temperature
Set Point Adjustment
0.1ºC
End of Program Cooling
Below Ambient Temperature
Yes to 4°C
Auto Restart on Power Failure Yes
Run End Time Calculation
Yes
Variable Programmable
Ramp Rate Steps
± 0.1ºC/sec
Connections and Dimensions
Serial Port RS 232 Socket
Yes
Voltage Selection Switch
Yes
Power Supply
230W
Dimensions (W x L x H)
185 x 305 x 190mm
Footprint
No more than 565cm2
Net Weight
No more than 5Kg
microtube interchangeable block
formats
• Fast-track programming with free
software upgrades available from the
Techne® website
• Ease of use - with modern, intuitive
programming and ready-to-go
templates, creating even the most
complicated protocols is simple
• Reliable, high performance with quality
guaranteed - borne from 20 years'
experience of manufacturing thermal
cyclers
• Portable Dual Voltage - a compact,
lightweight unit with a variable voltage
selector ensures that it can be
transported and used anywhere in the
world
• Unparalleled block uniformity - ±0.1ºC
at 50ºC, ensuring optimal reproducibility
*Uniformity is the typical variation in temperature that may occur
across the block under ambient conditions of 20ºC
• Fast heating rate - up to 3.6ºC/sec
heating rate and 2.0ºC/sec cooling rate
• Heated lid - a selectable temperature
range heated lid (+100ºC to +115ºC) is
sprung to fit both 0.2ml domed and
0.5ml flat top tubes
ORDERING INFORMATION
• Smallest footprint yet - at only 561cm2,
Complete System
TC-3000 thermal cycler for 25 x 0.2ml microtubes, 120V / 230V
TC-3000 thermal cycler for 20 x 0.5ml microtubes, 120V / 230V
Replacement Parts & Accessories
1 x TC-3000 block for 25 x 0.2ml microtubes
1 x TC-3000 block for 20 x 0.5ml microtubes
1 x PC connection to connect one thermal cycler
to PC (RS232 ONLY), includes cable and software
1 x one cycler cable extension set
1 x two cycler cable set
1 x four cycler cable set
1 x ten cycler cable set
1000 x 0.2ml microtubes
1000 x 0.5ml thin walled microtubes
Part No.
FTC3/02
FTC3/05
FTC3/02/B
FTC3/05/B
the TC-3000 is economical on space
• PC control & networking - can be
networked together with other Techne®
cyclers allowing up to 32 cyclers to be
connected to one PC
• Password protection - protects
FGEN232
FGENONE
FGENTWO
FGENFOUR
FGENTEN
FTUB02TW
FTUB05TW
programs against unwanted
modifications
• 4-year warranty
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Programming
Transilluminators
Biostep Transilluminators
Scie-Plas is pleased to offer the best selling products from the
Biostep transilluminator range. Biostep transilluminators, which
are available in single and dual wavelength formats
corresponding to the 254, 312 and 365nm UV wavebands.
• K-type - a basic, switch on/off model with constant UV
intensity
• E-type - a basic, switch on/off model with a 50/100% UV
intensity switch
• R-type - a basic, switch on/off model with a manual,
variable 10 to 100% UV intensity setting
• S-case - 325 x 322 x 105mm (W x D x H)
ORDERING INFORMATION
Replacement Parts & Accessories
Part No.
1 x 8 Watt tube, 254nm
BU55-W0103
1 x 8 Watt tube, 312nm
BU55-W0203
1 x 8 Watt tube, 365nm
BU55-W0403
1 x 2-sided UV protection shield for transilluminators
BU01-W8703
1 x 4-sided UV protection shield for transilluminators
BU01-W8705
1 x white-yellow UV conversion screen, 24 x 22cm (L x W)*
BC23-T2422
1 x white-yellow UV conversion screen, 32 x 27cm (L x W)*
BC23-T3227
1 x UV-transparent spot/band excision plate, 24 x 22cm (L x W)
BU53-W2422
1 x UV-transparent spot/band excision plate, 32 x 27cm (L x W)
BU53-W3227
*Suitable for gels stained with Coomassie® Blue, AmphiBlue® and Silver Stain;
autoradiograms and other transparent templates
100
TECHNICAL SPECIFICATION & ORDER INFORMATION
Type
Filter Size
Wavelength
UV Tubes
Other Features
Part No.
E
20 x 20cm
254nm
8 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-20S-8E
R
20 x 20cm
254nm
8 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-20S-8R
K
20 x 20cm
312nm
6 x 8 Watt
• homogeneous illumination
UST-20M-8K
E
20 x 20cm
312nm
8 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-20M-8E
R
20 x 20cm
312nm
8 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-20M-8R
E
23 x 30cm
312nm
12 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-30M-8E
R
23 x 30cm
312nm
12 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-30M-8R
K
20 x 20cm
365nm
6 x 8 Watt
• homogeneous illumination
UST-20L-8K
E
20 x 20cm
365nm
8 x 8 Watt
UST-20L-8E
R
20 x 20cm
365nm
8 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
UST-20L-8R
Dual Wavelength UV Transilluminators, S-case
Type
Filter Size
Wavelength
UV Tubes
Other Features
Part No.
K
20 x 20cm
254 / 312nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
USDT-20SM-8K
E
20 x 20cm
254 / 312nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
USDT-20SM-8E
R
20 x 20cm
254 / 312nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
USDT-20SM-8R
K
20 x 20cm
254 / 365nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
USDT-20SL-8K
K
20 x 20cm
312 / 365nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
USDT-20ML-8K
E
20 x 20cm
312 / 365nm
4 x 8 Watt / 4 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
USDT-20ML-8E
R
23 x 30cm
312 / 365nm
6 x 8 Watt / 6 x 8 Watt
• homogeneous illumination
• high-quality reflector and optimal tube positioning
• excellent proof sensitivity
• integrated cooling increases tube lifespan
USDT-30ML-8R
For a 110VAC electrical system add -A to the end of the Part No.
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Single Wavelength UV Transilluminators, S-case
102
Radioactive compounds have been used in research laboratories
for many years and the results of this research have benefited
mankind considerably. In life science research radioactive
compounds are used in the detection of nucleic acids and proteins,
as well as in other techniques such as radioimmunoassay and as
tracers in metabolic studies. The most commonly used isotopes
which emit Beta (β) radiation particles are [32P] phosphorus, [35S]
sulphur, [14C] carbon and [3H] tritium, while [125I] iodine, [133Xe]
xenon, [57Co] cobalt, [99Tcm] technetium, [123I] iodine and [67Ga]
gallium emit Gamma (γ) particles.
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Radiation Protection
Radiation Protection
SHIELDS models for sitting;
models for standing.
Compatible with safety trays.
STORAGE and TRANSPORT BLOCK
are ideal handling Eppendorf tubes
available for Beta and Gamma
radiation protection
BOXES are ideal for
storage of racked
samples or
contaminated
accessories
WORK RACKS and RACK
INSERTS fit both Gamma
and Beta storage blocks
SAFETY TRAYS provide
surface protection, spill
containment and a
clearly defined work area
SAFETY PRECAUTIONS
12mm thick lead-acrylic is suitable only
for the protection of Gamma emissions
from 125I and not from higher energetic
isotopes of iodine. Lead or lead-acrylic is
not recommended for shielding Beta
emissions. When a Beta particle collides
with an atom of a higher atomic number,
such as lead, a hazardous X-ray like
emission known as BREMSSTRAHLUNG is
generated. To avoid producing significant
amounts of bremsstrahlung, use only
optical clear acrylic products to shield
Beta emissions.
104
TIP-BINS and WASTE BINS are
a convenient solution for the
disposal of small contaminated
items
Radiation Protection
Generally, Beta emissions are negatively charged particles (electrons) emitted from the
nucleus of an atom. Depending on the particular atom, the energy level of the charged
particle will vary. Phosphorus is regarded as having a high energy level of Beta radiation
particles, being 10 times greater than that of tritium, which can travel up to seven metres
in air. Phosphorus is therefore known as a “hard” emitter of Beta radiation, while tritium,
sulphur and carbon are regarded as “soft” emitters of Beta radiation.
[125I] Iodine is a weak emitter of Gamma radiation particles. Gamma emissions are a form
of electromagnetic radiation, similar to that of light generated by the nuclear decay of an
unstable isotope. They are much more penetrating than Beta emissions and can travel
very long distances in air.
BETA PROTECTION PRODUCTS
Beta emissions of 32P, 35S, 3H and 14C can be effectively blocked by using 10mm thick
optical acrylic, which is now the accepted standard for shielding Beta emissions in
biological research. The properties of this acrylic material are ideal for the construction
of Beta radiation protection products. It is easy to bend, fabricate and machine into a
wide range of products. Also, being transparent, the visibility of the working environment
is not impaired.
GAMMA PROTECTION PRODUCTS
Protection from Gamma emitting isotopes
such as 125I, 133Xe, 57Co, 99Tcm, 123I and 67Ga
has, until recently, been achieved by lead
shielding. This has its obvious
disadvantages, being very heavy and nontransparent. However, a new lead
containing acrylic copolymer resin is 30%
wt/wt and is chemically introduced into
the acrylic resin as an organolead salt.
This material is transparent with a very
light brown tint and it exhibits virtually all
the normal chemical and physical
properties of conventional acrylic resin.
Scie-Plas Limited provide a range of
products using 12mm thick lead-acrylic,
which is equivalent to 0.5mm thick lead.
This range of products effectively blocks
Gamma emissions from 125I and any
Gamma emitters of lesser energy. It is
NOT suitable for use with more energetic
isotopes of iodine. However, a 35mm thick
shield is available which is equivalent to
1.7mm thick lead. This can be used with
isotopes of higher energy
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Scie-Plas Limited manufacture the complete range of radiation protection products
shown in this catalogue. Many styles and sizes of apparatus are listed for use with
different applications.
Radiation Protection
BENEFITS INCLUDE
• 10mm clear optical acrylic or 12mm
lead acrylic for unobstructed view
• Choice of angle to suit location
• Most shields available in two sizes small and large
• Large flat base models for underbench protection
• Curved base models are compatible
with Scie-Plas safety tray
Shielding
Many different styles and sizes of shield are available depending on
the requirements of the user. Most shields are available in two
sizes, small 450 x 300mm and large 530 x 350mm, and have either
a large flat base, 300mm deep, for under-bench protection or a
curved base, 120mm deep, for use with a Scie-Plas safety tray.
Adjustable 0 to 15° angle
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
Dimensions
(upright and
horizontal)
RPP-S015
540 x 350mm
Base
350 x 540mm
RPP-GS015
540 x 350mm
Base
350 x 540mm
These shields are easily adjusted from an upright position to an angle of 15° depending
on the application required. They can also be used in either wide or a tall position.
When configured in a horizontal position, it creates a convenient side shield. The
standard 12mm thick Gamma shield can be used with 125I
ORDERING INFORMATION
Adjustable angle Beta shield
Adjustable angle 12mm thick Gamma shield
106
RPP-S015
RPP-GS015
FEATURES AND BENEFITS
• 10mm clear optical acrylic or 12mm
lead acrylic for an unobstructed
view
• Most shields available in two sizes small and large
• Large flat base models for underbench protection
• Curved base models are compatible
with Scie-Plas safety trays.
Fixed Angle Shielding
Fixed 15° angle shields provide clear, non-distorted vision, reducing light images and
shadows especially when the user is in a seated position. They are available in two
sizes, small or large, with either a large flat base to provide under bench protection or
a curved base, which fits conveniently over safety trays.
ORDERING INFORMATION
Small fixed
Small fixed
Small fixed
Large fixed
Large fixed
Large fixed
Large fixed
15° angle shield 15° angle shield 15° angle shield 15° angle shield 15° angle shield 15° angle shield 15° angle shield -
flat base,
Beta
flat base,
Gamma
curved base, Gamma
flat base,
Beta
curved base, Beta
flat base,
Gamma
curved base, Gamma
RPP-S15S
RPP-GS15S
RPP-GS15SC
RPP-S15L
RPP-S15LC
RPP-GS15L
RPP-GS15LC
Beta 10mm acrylic
Gamma 12mm lead acrylic
Dimensions
(upright and
horizontal)
RPP-S15S
450 x 300mm
Base
300 x 300mm
RPP-GS15S
450 x 300mm
Base
300 x 300mm
RPP-GS15SC
450 x 300mm
Base
150 x 300mm
RPP-S15L
530 x 350mm
Base
350 x 300mm
RPP-S15LC
530 x 350mm
Base
150 x 350mm
RPP-GS15L
530 x 350mm
Base
350 x 300mm
RPP-GS15LC
530 x 350mm
Base
150 x 350mm
Hourglass Shields
Hourglass shields have identical features to the fixed 15° angle shields but are cut in
an hourglass shape. The curved sides provide the user with easier access to the
central area behind the shield.
ORDERING INFORMATION
Hourglass shield - flat base, Beta
RPP-SH
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Dimensions
(upright and
horizontal)
RPP-SH
450 x 300mm
Base
300 x 300mm
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TECHNICAL SPECIFICATION
Radiation Protection
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
RPP-S45S
Base
RPP-S45SC
Base
RPP-GS45SC
Base
RPP-S45L
Base
Dimensions
(upright and
horizontal)
450 x 300mm
300 x 300mm
450 x 300mm
150 x 300mm
450 x 300mm
150 x 300mm
600 x 350mm
350 x 300mm
Fixed 45° Angle Shields
Fixed 45° angle shields provide clear, non-distorted vision reducing light images and
shadows, especially when the user is in a standing position. They are available in two
sizes, small or large, with either a large flat base to provide under bench protection or
a curved base, which fits conveniently over safety trays.
ORDERING INFORMATION
Small fixed 45° angle shield - flat base, Beta
Small fixed 45° angle shield - curved base Beta
Small fixed 45° angle shield - curved base Gamma
Large fixed 45° angle shield - flat base Beta
TECHNICAL SPECIFICATION
Beta 10mm acrylic
RPP-SB
RPP-S45S
RPP-S45SC
RPP-GS45SC
RPP-S45L
3-Sided Shield
Dimensions
(upright and
horizontal)
500 x 460 x 300mm
A 3-sided shield can be used with our range of Beta shields, providing further all round
protection.
ORDERING INFORMATION
3-sided shield, Beta
108
RPP-SB
Storage Boxes
Scie-Plas Ltd manufactures a
wide range of boxes and bins
for the storage of samples and
waste materials.
Interchangeable inserts
between the boxes can hold a
variety of vessels of different
sizes. A range of heavy-duty
waste bags with drawstrings for
easy sealing are available to fit
inside the waste bins.
The smallest box in our range has a hinged lid and is ideal for storage when space is
limited. Interchangeable box inserts are available for 16 x 1.5ml Eppendorf tubes.
TECHNICAL SPECIFICATION
Beta 10mm acrylic
ORDERING INFORMATION
Mini-box with hinged lid, Beta
Mini-box insert - 16 x 1.5ml Eppendorf tubes
RPP-B5
RPP-B5
RPP-BI-16
Dimensions
(H x W x D)
Ext
Int
RPP-BI-16
75 x 105 x 105mm
55 x 85 x 85mm
40 x
80 x 80mm
Midi-Boxes with Hinged Lids
The midi-box has a hinged lid and is designed to contain interchangeable inserts,
which hold a double number of microcentrifuge tubes as the mini-box, as well as 32 x
2ml cryotubes. This model is available for either Beta or Gamma radiation protection.
RPP-BI-32
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
ORDERING INFORMATION
Midi-box with hinged lid, Beta
Midi-box with hinged lid, Gamma
Midi-box insert - 32 x 1.5ml Eppendorf tubes
RPP-B6
RPP-GB6
RPP-BI-32
Dimensions
(H x W x D)
RPP-B6
Ext
Int
80 x 185 x105mm
60 x 165 x 85mm
RPP-GB6
Ext
Int
84 x 189 x 109mm
60 x 165 x 85mm
RPP-BI-32
40 x 160 x 80mm
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Beta Mini-Box with Hinged Lid
Radiation Protection
Maxi-Boxes with Hinged Lids
The maxi-box is designed to hold up to three midi-box inserts as well as inserts for
taller tubes. This model is available for either Beta or Gamma radiation protection.
RPP-BI-6
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
RPP-B14
RPP-GB14
RPP-BI-6
Dimensions
(H x W x D)
RPP-B14
Ext 160 x 300 x 185mm
Int 140 x 280 x 165mm
RPP-GB14
Ext 164 x 304 x 189mm
Int 140 x 280 x 165mm
RPP-BI-6
Maxi-box with hinged lids, Beta
Maxi-box with hinged lids, Gamma
Maxi-box insert - 15 x 5ml scintillation vials
40 x 160 x 80mm
Box with 2 “Pig Pens”,
Inserts and Hinged Lids
This box features two completely isolated chambers, each with their own hinged lid.
Each chamber contains a removable insert for the safe support of vials.
TECHNICAL SPECIFICATION
Beta 10mm acrylic
RPP-BX1
Dimensions
(H x W x D)
90 x 120 x 80mm
ORDERING INFORMATION
2 pig-pen box, Beta
RPP-BX1
Storage and
Transport Block
TECHNICAL SPECIFICATION
Beta 10mm acrylic
RPP-STB
Dimensions
(H x W x D)
A storage and transport block manufactured from solid acrylic specifically machined to
accept 24 x 1.5ml and 15 x 0.5ml Eppendorf tubes.
70 x 150 x 120mm
ORDERING INFORMATION
Storage and transport box, Beta
110
RPP-STB
Waste Bins
•
Suitable for low level
radioisotope waste
•
Hinged lid
•
Durable PVC hinges
Beta and Gamma Bins
ORDERING INFORMATION
Bin-12, Beta (1L capacity)
Bin-17, Beta (3.3L capacity) use waste bags RPP-BAG17
Bin-17, Gamma (3.3L capacity) use waste bags RPP-BAG17
Bin-27, Beta (10L capacity) use waste bags RPP-BAG31
Bin-27, Gamma (10L capacity) use waste bags RPP-BAG31
Bin-31, Beta (15L capacity) use waste bags RPP-BAG31
Bin-42, Beta (50L capacity) use waste bags RPP-BAG42
Bin-60, Beta (47L capacity) use waste bags RPP-BAG60
Bin-76, Beta (122L capacity) use waste bags RPP-BAG60
RPP-B12
RPP-B17
RPP-GB17
RPP-B27
RPP-GB27
RPP-B31
RPP-B42
RPP-B60W
RPP-B76W
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
Dimensions
(H x W x D)
1L
RPP-B12
Ext 150 x 120 x 100mm
Int 130 x 100 x 80mm
3.3L
RPP-B17
Ext 170 x 170 x 170mm
Int 150 x 150 x 150mm
3.3L
RPP-GB17
Ext 174 x 174 x 174mm
Int 150 x 150 x 150mm
10L
RPP-B27
Ext 270 x 220 x 220mm
Int 250 x 200 x 200mm
10L
RPP-GB27
Ext 274 x 224 x 224mm
Int 250 x 200 x 200mm
15L
RPP-B31
Ext 315 x 235 x 255mm
Int 295 x 215 x 235mm
50L
RPP-B42
Ext 420 x 510 x 290mm
Int 400 x 490 x 270mm
47L
RPP-B60W
Ext 600 x 305 x 290mm
Int 580 x 285 x 270mm
122L
RPP-B76W
Ext 760 x 426 x 426mm
Int 740 x 406 x 406mm
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Scie-Plas Ltd manufactures a range of bench-top bins for storing radioactive
materials. All of the bins have hinged lids and cushioned anti-slip feet. Two large size
Beta storage bins with hinged lids have wheels for easy manoeuvrability.
Three sizes of bins are also available for Gamma radiation protection. A range of
heavy-duty plastic bags with drawstrings are available to fit conveniently inside all of
our bin sizes.
Radiation Protection
Boxes
•
For the disposal of used pipette tips
- additional hole/cover reduces
exposure.
•
Small and large models
•
Waste bags available for easy
removal of contents
•
Multi-tip boxes feature a slot in the
lid, to allow disposal of tips from a
multi-channel pipette
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
RPP-B15SC
Ext 150 x 150 x 150mm
Int 130 x 130 x 130mm
2L
RPP-GB15SC
Ext 150 x 150 x 150mm
Int 126 x 126 x 126mm
TECHNICAL SPECIFICATION
RPP-BTB
Dimensions
(H x W x D)
Ext
Int
Available for either Beta or Gamma radiation protection, these boxes have a hinged lid
with a central hole/slot also covered by a smaller hinged lid. The central hole/slot
reduces the exposure to radiation during pipetting procedures. Models are available for
single or multi-tip disposal. Convenient disposable heavy-duty plastic bags are
available.
ORDERING INFORMATION
Dimensions
(H x W x D)
2L
Beta 10mm acrylic
Beta and Gamma tip boxes
95 X 315 X 400mm
75 x 295 x 280mm
Tip box-15, Beta (2L capacity) use waste bags RPP-BAG17
Tip box-15, Gamma (2L capacity) use waste bags RPP-BAG17
Multi-tip box-15, Beta (2L capacity) use waste bags RPP-BAG17
Multi-tip box-15, Gamma (2L capacity) use waste bags RPP-BAG17
RPP-B15SC
RPP-GB15SC
RPP-B15SCMC
RPP-GB15SCMC
Beta Transit Box
This transit box combines a shield with a conveniently contained work area and storage
container.
The hinged lid creates a 15° angled shield when fully open. The lid can be locked shut
for safety while transporting the unit and its contents. Side handles are fitted for easy
lifting and cushioned non-slip feet prevent slipping.
ORDERING INFORMATION
Transit box, Beta
112
RPP-BTB
Beta Carboy Cover
TECHNICAL SPECIFICATION
A simple acrylic cover for the protection of carboys and
laboratory equipment. An open base allows the cover to be
placed over the container or equipment. It can also be
removed without disturbing the container. A hinged lid
provides easy access. The cover can be used in conjunction
with a laboratory safety tray.
Beta 10mm acrylic
RPP-CBC
Dimensions
(H x W x D)
Ext
Int
600 x 400 x 400mm
590 x 380 x 380mm
ORDERING INFORMATION
RPP-CBC
Hazard Signs
A range of radiation hazard adhesive signs and tapes.
ORDERING INFORMATION
Radiation hazard symbol labels
25 x 25mm, pk/25
Radiation hazard symbol labels
50 x 50mm, pk/50
Radiation hazard tape, roll
25mm x 66m
RPP-LAB25
RPP-LAB50
RPP-TAPE
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Carboy cover, Beta
500mm
Radiation Protection
390mm
570mm
570mm
•
All-round protection for all
operators and those working in
nearby areas
•
Compatible with Scie-Plas safety
tray (not included)
•
Ergonomically designed
•
Hinged doors
Cabinet Workstation
This cabinet has two large side doors to provide easy access for the user. It can also be
used as a fully enclosed workstation. The dimensions are such that a sizeable working
space is provided and the user’s vision is unobstructed whether sitting or standing. A
small cut-out at the rear of the unit enables services to be used inside the cabinet. When
not in use, the doors can be closed and the unit used to store radioactive material.
The rear face features a convenient holder for two pipettes, which helps to keep the work
surface clear, while services can be accessed through the cut-out at the base of the
cabinet. The cabinet has no base in order for it to be used on a removable 5mm thick
acrylic base tray. A suitable laboratory safety tray, RPP-TY6854 or RPP-TW6854, can also
be used. Available for either Beta or Gamma radiation protection.
ORDERING INFORMATION
TECHNICAL SPECIFICATION
Beta 10mm acrylic
Gamma 12mm lead acrylic
Dimensions
(H x W x D)
RPP-C
Ext 500 x 570 x 390mm
Int 490 x 550 x 370mm
RPP-GC
Ext 500 x 570 x 390mm
Int 488 x 446 x 366mm
Cabinet, Beta
Cabinet, Gamma
Base tray for cabinet, white 68 x 54cm
Base tray for cabinet, yellow 68 x 54cm
Tray liners for cabinet, pk/25 68 x 54cm
RPP-C
RPP-GC
RPP-TW6854
RPP-TY6854
RPP-TL6854
Radiation Safety Bags
Heavy-duty, double heat-sealed 500 gauge polyethene with double sting neck pull.
For use with Scie-Plas Beta and Gamma bins.
ORDERING INFORMATION
Bag 17
Bag 31
Bag 35
Bag 40
Bag 42
Bag 60
114
120 x 120 x 120mm, pk/25
290 x 210 x 210mm, pk/25
330 x 120 x 120mm, pk/25
370 x 210 x 240mm, pk/25
400 x 490 x 270mm, pk/25
610 x 280 x 280mm, pk/25
RPP-BAG17
RPP-BAG31
RPP-BAG35
RPP-BAG40
RPP-BAG42
RPP-BAG60
Safety Trays and Liners
Unlike disposable paper bench protectors, Scie-Plas safety trays and tray liners are reusable. This reduces running costs and drastically reduces contaminated waste volume.
The rigid PVC base features specially designed stabilising edges and rounded corners for
easy cleaning. Additionally, the liner’s non-porous surface allows valuable samples to be
retrieved - something which is clearly not possible with absorbent paper protectors.
•
Saves on running cost
•
Contains liquid spillages
•
Provides clearly defined work
area
•
Easily cleaned
•
APET - environmentally friendly
when incinerated
•
Suitable for most hazardous
spillages
•
Anti-static and non-porous
ORDERING INFORMATION
Tray Type
General
Purpose
Radiation
Hazard
Biohazard
Ext Dimensions
Int Dimensions
Base only
46 x 26cm
54 x 34cm
57 x 54cm
68 x 54cm
70 x 46cm
113 x 54cm
46 x 26cm
54 x 34cm
57 x 54cm
68 x 54cm
70 x 46cm
113 x 54cm
46 x 26cm
54 x 34cm
57 x 54cm
68 x 54cm
70 x 46cm
113 x 54cm
40.5 x 20cm
46 x 26cm
45.5 x 43cm
56.5 x 42.5cm
57 x 35cm
100 x 42cm
40.5 x 20cm
46 x 26cm
45.5 x 43cm
56.5 x 42.5cm
57 x 35cm
100 x 42cm
40.5 x 20cm
46 x 26cm
45.5 x 43cm
56.5 x 42.5cm
57 x 35cm
100 x 42cm
RPP-TW4626
RPP-TW5434
RPP-TW5754
RPP-TW6854
RPP-TW7046
RPP-TW11354
RPP-TY4626
RPP-TY5434
RPP-TY5754
RPP-TY6854
RPP-TY7046
RPP-TY11354
RPP-TO4626
RPP-TO5434
RPP-TO5754
RPP-TO6854
RPP-TO7046
RPP-TO11354
APET Liners
pk/25
RPP-TL4626
RPP-TL5434
RPP-TL5754
RPP-TL6854
RPP-TL7046
RPP-TL11354
RPP-TL4626
RPP-TL5434
RPP-TL5754
RPP-TL6854
RPP-TL7046
RPP-TL11354
RPP-TL4626
RPP-TL5434
RPP-TL5754
RPP-TL6854
RPP-TL7046
RPP-TL11354
115
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Safety Trays
Laboratory Equipment
Laboratory Equipment
Serological Pipette Rack
These racks are for bench top storage of manual glass or plastic serological pipettes of
0.1ml to 25ml capacity. They have four angled compartments measuring 8 x 8 x 30cm.
being fabricated from 5mm clear acrylic, stock levels of pre-packed pipettes can be
constantly monitored. Additionally, each compartment can accept standard pipette cans
and allow one-handed retrieval of pipettes when working in a biological hood.
ORDERING INFORMATION
Pipette rack - four shelves
GLE-PR
Coloured Tape Dispensers
Scie-Plas tape dispensers are ideal for easy one-handed access to popular coloured
identification tapes. A serration on the dispensing edge allows the tape to be easily torn off.
Each dispenser will take up to 12 rolls of the most popular 12.7mm wide tapes. Of smoked
acrylic construction, two models are available for tapes of differing core diameters.
ORDERING INFORMATION
Small tape dispenser, tape core diameter 26mm
Large tape dispenser, tape core diameter 76mm
DISP26MM
DISP76MM
Glass Plate Racks
These sturdy racks are designed for safe drying and storage of glass
plates. The small rack can hold up to twenty 2mm thick plates,
while the larger rack can take up to ten 4mm thick glass plates.
ORDERING INFORMATION
Mini glass plate rack for twenty 2mm plates
Large glass plate rack for ten 4mm plates
116
GLE-MGR
GLE-LGR
This range of products has been
continuously expanded and offers a variety
of reagents for electrophoresis, including
solid gel media, gel solutions, precast
gels, ready-to-use buffers and solutions,
as well as stains, dyes and markers.
Only available in EU Countries
Electrophoresis Reagents and Buffers
AGAROSE GEL REAGENTS AND BUFFERS
Agarose is a highly purified, naturally-occurring polysaccharide.
The preparation of agarose gels involves simply heating the powdered
agarose in buffer to dissolve it. It will then gel upon cooling. Like
acrylamide the pore size of an agarose gel is inversely dependent on the
agarose concentration. The pores in agarose gels are generally much
larger than those in acrylamide gels, making them suitable for the
separation of much larger nucleic acid fragments. There are many types
of agarose available. The best choice for routine DNA electrophoresis
is Agarose for DNA Electrophoresis (Catalogue Number 11404). This
offers good gel strength and low impurities that might interfere with
subsequent procedures.
117
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Electrophoresis
reagents, buffers,
gels & membranes
Reagents
Agarose, DNA Electrophoresis Grade
For analytical and preparative nucleic acid electrophoresis.
Each batch is tested for the absence of EcoR1 inhibition.
POLYACRYLAMIDE GEL REAGENTS
AND BUFFERS
Agarose, Electrophoresis Grade
Agarose, Electrophoresis Grade
Agarose, Electrophoresis Grade
ACRYLAMIDE-BIS SOLUTIONS
100g
500g
1000g
11404.03
11404.07
11404.05
Agarose, Premium Molecular Biology Grade
Agarose, Molecular Biology Grade
100g
11381.02
TBE Buffer (10x)
TBE buffer is widely used in molecular biology and nucleic acid
electrophoresis and has a higher buffering capacity than TAE buffer.
It can be used for DNA and RNA, polyacrylamide and agarose gel
electrophoresis. Supplied as a 10x concentrate.
(0.89M Tris, 0.89M Boric Acid, and 0.02M EDTA in aqueous solution).
TBE Buffer, 10x
1L
42557.01
TAE Buffer (10x)
TAE buffer is used for the electrophoresis of nucleic acids. TAE has
a lower buffering capacity than TBE, although linear dsDNA tends
to run faster in TAE than in TBE buffer.
Supplied as a 10x concentrate.
(0.4M Tris, 0.2M Acetic Acid, 0.01M EDTA in aqueous solution).
TAE Buffer, 10x
1L
42553.01
DNA Marker, Lambda x BstE II
The Lambda x BstE II DNA marker contains 14 fragments, ranging
from 117 to 8,454 bp: 117, 224, 702, 1264, 1371, 1929, 2323, 3675,
4324, 4822, 5687, 6369, 7242, and 8454 bp (the 5687 bp and 8453 bp
fragments contain the cohesive ends of bacteriophage lambda and
may hybridise resulting in a high molecular weight band at 14140
bp, although the ends may be separated by heating to 65°C for 5
minutes and placing on ice). Ideal for the analysis of DNA
fragments generated from genomic or plasmid DNA.
DNA Marker, Lambda x BstE II
2 x 50µg
The purity of acrylamide and bis-acrylamide is an
important variable in gel electrophoresis. The most
significant impurities are acrylic acid and
polyacrylamide. The former can affect pH control in the
gel while the latter influences the total polyacrylamide
content of the gel and therefore its sieving properties.
Since both of these can form on standing from highly
purified material, especially with exposure to light, it is
important to store these products protected from light
and cold.
39301.01
SERVA acrylamide solutions are prepared from
powdered material which is subject to stringent
quality control of the critical parameters in order to
ensure consistent and reliable results.
Acrylamide-Bis Solution, 29:1, (40% w/v) 3.3% C
Solution of acrylamide and bis N,N'-methylenbisacrylamide in
deionised water. Convenient to use, with reduced risk of
exposure to neurotoxic acrylamide dust. Applicable to all
electrophoresis techniques.
Acrylamide:Bis Solution, 29:1 (40% w/v) 500ml
Acrylamide-Bis Solution, 37.5:1, (30% w/v) 2.6% C
Solution of acrylamide and bis N,N'-methylenbisacrylamide in
deionised water. Convenient to use, with reduced risk of
exposure to neurotoxic acrylamide dust. Applicable to all
electrophoresis techniques.
Acrylamide:Bis Solution, 37.5:1 (30% w/v) 500ml
Ethidium Bromide - Aqueous Solution, 1% w/v
Suitable for use in DNA isolation procedures and in the staining of
DNA after electrophoresis.
Concentration: 10mg/ml
REAGENTS AND BUFFERS
Ethidium Bromide, Aqueous Solution, 1% w/v.
Ammonium Persulphate (APS)
25ml
21251.01
pk/5
Ammonium Persulphate (APS), Analytical Grade
A high speed initiator used in polyacrylamide gel electrophoresis.
TEMED
50g
13375.01
90-1500
100ml
35925.01
Laemmli Electrophoresis Buffer (10x)
A tris-glycine/SDS electrophoresis buffer supplied as a 10x
concentrate for SDS-PAGE. (0.25M Tris, 1.92M Glycine and 1% SDS
in aqueous solution).
Laemmli Buffer 10x
118
10688.01
N,N,N',N'-Tetramethylethylenediamide (TEMED)
Catalyses the formation of free radicals by ammonium persulphate
and accelerates the polymerisation of acrylamide and bis-acrylamide.
Ethidium Bromide Destaining Bags
Each bag will remove up to 5mg of Ethidium
Bromide from solution, from an overnight
preparation. The rate of destaining is
improved if more destaining bags are added
to the solution.
Ethidium Bromide - Destaining Bags
10680.01
2L
42556.01
Tris-Glycine/SDS Sample Buffer, 2x
20ml
42527.01
Tris-Tricine/SDS Electrophoresis Buffer (10x)
A tris-tricine/SDS electrophoresis buffer supplied as a 10x
concentrate for SDS-PAGE. (1M Tris, 1M Tricine and 1% SDS in
aqueous solution).
Tris-Tricine/SDS Electrophoresis Buffer,10x 1L
42552.01
Tris-Tricine/SDS Sample Buffer (2x)
A tris-tricine/SDS sample buffer supplied as a 2x concentrate.
(Tris-HCl pH 8.45, 90mM, Glycerol 24%, SDS 4%, SERVA Blue G
0.015%, Phenol Red 0.005%).
Tris-Tricine/SDS Sample Buffer, 2x
20ml
42551.01
Protein Marker, Unstained SDS-PAGE, 6.5 - 200 KDa, Liquid Mix
Ready to use for SDS-PAGE. Standard proteins ranging from 6.5
to 200 KDa. Protein content is approximately 0.15 to 0.3mg/ml.
Myosin
B-Galactosidase
Albumin Bovine (BSA)
Ovalbumin
Carbonic Anhydrase
Trypsin Inhibitor (soybean)
Lysozyme
Aprotinin
MW
MW
MW
MW
MW
MW
MW
MW
Protein Marker, 6.5 to 200KDa
500µl
200,000
116,000
67,000
45,000
29,000
21,000
14,400
6,000
CoZap™ Coomassie Blue Destainer
CoZap™ is used for the rapid removal of Coomassie Blue stain
from electrophoresis gels without the need to change the
destaining solution. CoZap™ is a unique pad that has high
absorbance for Coomassie Blue and is thus very effective in
destaining gels. CoZap™ absorbs any free dye in the solution,
making gel destaining 20% faster than conventional methods.
BENEFITS INCLUDE
• No need to change the destaining solution
• Fast and simple
• No charcoal or dye residues
• No subsequent destaining required
• One pad can destain up to 10 gels
• 20% faster than conventional methods
CoZap™ - Coomassie Blue
Destaining Pads, 76 x 76 x 2mm
CoZap™ - Coomassie Blue
Destaining Pads, 76 x 76 x 2mm
CoZap™ - Coomassie Blue
Destaining Pads, 38 x 76 x 2mm
CoZap™ - Coomassie Blue
Destaining Pads, 38 x 76 x 2mm
pk/25
746800
pk/100
746801
pk/100
746802
pk/250
746803
Colloidal Coomassie Blue Staining Wash
39215.01
Colloidal Coomassie Blue (CCB)
Coomassie Blue G250 is widely used in visualising proteins
separated by either agarose or acrylamide gel electrophoresis. This
normally involves lengthy staining and destaining of gels using both
glacial acetic acid and methanol. These solvents are both toxic and
poisonous and need to be disposed as hazardous waste.
In order to avoid using these materials, Coomassie Blue G250 has
been formulated into a colloid (CCB), which is both non-toxic and
non-hazardous. The colloidal solution represents a safer way to
apply the dye to the gels and is both easy and less expensive to use.
The CCB stain is applied to the gel after washing the gel with
purified deionised water. The CCB solution is then agitated until
the bands start to appear within the gel, any time from 20 minutes
to two hours.
Once the bands appear and are of sufficient intensity, simply pour
off the CCB stain and wash the gel in water. Keep washing until
the background staining is removed and the protein bands appear
more intense. This can be further enhanced by using CoZap™, a
quick and easy destainer for Coomassie Blue.
Colloidal Coomassie Blue
1L
30-38-10
119
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Tris-Glycine/SDS Sample Buffer (2x)
A tris-glycine/SDS sample buffer supplied as a 2x concentrate. (Tris-HCl
pH 6.8, 126mM, glycerol 20%, SDS 4%, bromophenol blue 0.02%).
Reagents
Isoelectric Focusing
Isoelectric Focusing (IEF) is an ingenious process for
simultaneous concentration and separation of
proteins. IEF employs a pH gradient formed by small
amphoteric molecules (ampholytes) to resolve
proteins according to their different pI (isoelectric
point) values. IEF is an end-point method; when the
electrophoresis run is completed proteins will appear
as separate sharp zones, in the order of their
isoelectric point.
SERVALYT™ Carrier Ampholytes:
Overview & Ordering Information
pH range
Cat. No.
Quantity
Cat. No.
Quantity
2-4
42902.01
10 ml
42902.02
25 ml
2-9 Seed Mix
42935.01
42935.03
10 ml
100 ml
42935.02
25 ml
2-11
42900.01
10 ml
42900.02
25 ml
3-4
42922.01
10 ml
42922.02
25 ml
3-5
42903.01
10 ml
42903.02
25 ml
3-6
42944.01
10 ml
42944.01
25 ml
3-7
42945.01
10 ml
42945.01
25 ml
3-10
42940.01
10 ml
42940.02
25 ml
3-10 Iso-Dalt *
42951.01
10 ml
42951.01
25 ml
4-5
42923.01
10 ml
42923.02
25 ml
4-6
42904.01
10 ml
42904.02
25 ml
4-7
42948.01
10 ml
42948.02
25 ml
4-9 T **
42910.01
42910.03
10 ml
100 ml
42910.02
25 ml
5-6
42924.01
10 ml
42924.02
25 ml
BENEFITS INCLUDE
5-7
42905.01
10 ml
42905.02
25 ml
• High resolution due to multimeric composition
• Fast staining and destaining times
• Clear background associated with very low unspecific binding of
dyes and stains
• High solubility in trichloroacetic acid (fast removal of ampholytes
during fixation)
• Virtually no interaction with metal ions
5-7 PGM
42936.01
10 ml
42936.02
25 ml
5-8
42949.01
10 ml
42949.02
25 ml
5-9
42950.01
10 ml
42950.02
25 ml
6-7
42925.01
10 ml
42925.02
25 ml
6-8
42906.01
10 ml
42906.02
25 ml
6-9
42913.01
10 ml
42913.02
25 ml
7-9
42907.01
10 ml
42907.02
25 ml
9-11
42909.01
10 ml
42909.02
25 ml
Scie-Plas offer the following products for IEF: SERVALYTTM Carrier Ampholytes
SERVALYTTM Carrier Ampholytes are low molecular weight molecules
of zwitterionic character. They are a mixture of synthetically derived
species of average molecular weight distribution of 400 to 1000 Da
and comprise a multitude of varying pI values. In agarose and
polyacrylamide gels containing ampholytes, a linear pH gradient is
generated when an electric field is applied. The ampholyte molecules
carry a net charge and thus migrate in the electric field between the
electrodes until they reach the position of their corresponding pI,
when they stop moving and form small plateaus (stationary stacks).
* Iso-Dalt quality; special 2-D grade to be used in 2-D electrophoresis
** Technical grade quality for preparative work
120
With SERVALYT™ PRECOTES™ precast gels, the days
of elaborate, inconvenient casting of gels are gone.
Scie-plas offers ready-to-use polyacrylamide gels for
horizontal IEF that are simple to handle and reliable in
performance.
The polyacrylamide layer is bonded to a sturdy, inert
polyester support film and protected from damage and
drying out by a thin cover sheet. The polymer
concentration is 5% and cross-linking is 3%.
BENEFITS INCLUDE
• Reliable, reproducible results
• Easy to use
• Thin (0.3mm) and Ultra-thin (0.15mm) gels
• 3 Gel formats, Large - 245 x 125mm, Standard 125 x 125mm, Mini - 45 x 50mm (width x height)
• Mini gels, compatible with automated electrophoresis units
• Long shelf life: over 12 months
Meat
SERVALYT™ PRECOTES™ - Precast IEF Gels
SERVALYT™ PRECOTES™ pH 3 - 10
5 gels
0.15mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 3 - 6
5 gels
0.15mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 4 - 6
5 gels
0.15mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 5 - 7
5 gels
0.15mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 6 - 9
5 gels
0.15mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 3 - 10
5 gels
0.3mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 3 - 6
5 gels
0.3mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 4 - 6
5 gels
0.3mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 5 - 7
5 gels
0.3mm thick, Size 125 x 125mm
SERVALYT™ PRECOTES™ pH 6 - 9
5 gels
0.3mm thick, Size 125 x 125mm,
SERVALYT™ PRECOTES™ pH 3 - 10
5 gels
0.15mm thick, Size 245 x 125mm
SERVALYT™ PRECOTES™ pH 3 - 6
5 gels
0.15mm thick, Size 245 x 125mm
SERVALYT™ PRECOTES™ pH 4 - 6
5 gels
0.15mm thick, Size 245 x 125mm
SERVALYT™ PRECOTES™ pH 6 - 9
5 gels
0.15mm thick, Size 245 x 125mm
SERVALYT™ PRECOTES™ pH 3 - 10
5 gels
0.3mm thick, Size 245 x 125mm
42965.03
42974.02
42975.02
42979.02
42978.02
42866.02
42874.02
42875.02
42879.02
42878.02
42967.02
42919.03
42942.03
42954.02
42867.02
Potato
Blank SERVALYT™ PRECOTES™ Precast IEF Gels
Blank PRECOTES™ were developed to provide a versatile solution to
perform isoelectric focusing of any pH range. The Blank
PRECOTES™ are equilibrated in the ampholyte mixture of choice for
30 minutes prior to electrophoresis.
SERVALYT™ PRECOTES™ STARTER KIT
The SERVALYT™ PRECOTES™
STARTER KIT contains 3 x
SERVALYT™ PRECOTES™ pH 3 - 10 precast gels, electrode wicks,
applicator strips, anode and cathode solutions, Bayol F, SERVA Blue
W tablets, lyophilised IEF marker 3 - 10 and instructions for IEF and
staining.
Blank PRECOTES™, Gel size 125 x 125 x 0.3mm, 5 gels 42759.01
Blank PRECOTES™, Gel size 245 x 125 x 0.3mm, 5 gels 42710.01
SERVALYT™ PRECOTES™
Starter Kit
EACH
39060.01
121
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Precast Gels for Isoelectric
Focusing
Reagents
PROTEIN MARKER FOR ISOELECTRIC FOCUSING
The Liquid Mix IEF Marker pH 3 - 10 is a ready-to-use protein marker
especially developed for IEF applications. The marker is applicable to
all horizontal and vertical IEF gels and can be used in both native and
denaturing conditions (e.g. containing 8M). The marker is an ideal tool
for the determination of the pI (isoelectric point) of unknown protein
samples, but also for monitoring separation performance of IEF gels.
The marker is supplied in 10% glycerol containing Bromophenol Blue
(0.01%) and Methyl Red (0.01%).
BENEFITS INCLUDE
• Ready-to-use or lyophilised protein marker for isoelectric
focusing
• For pI determination of unknown protein samples
Other Reagents
Bayol F
Bayol F
100ml
14500.01
1L
26940.01
Kerosene Pure
Kerosene
IPG BlueStrips Dried gel strips with
immobilised pH gradient
• For monitoring the separation performance of IEF gels
• One standard applicable to all IEF gels (vertical/horizontal)
• No need for extra “High pI range” or “Low pI range” markers
• Purified protein components, salt-free
• 13 isoforms, featuring a characteristic pattern
Liquid IEF Marker pH 3 - 10
Ready-to-use
Lyophilised IEF Marker pH 3 - 10
BENEFITS INCLUDE
5mg
39212.01
• Stored at -20°C the gel strips are stable for at least 18
months
10mg
39211.01
• Each package contains 12 gel strips, 3.0mm wide
ACCESSORIES FOR ISOELECTRIC FOCUSING
Applicator Strips
Four different applicator strips are available for dispensing samples.
Applicator Strips 2 x 3.5
19 slots, 100mm long
Applicator Strips 3.5 x 2
15 slots, 100mm long
Applicator Strips 7 x 1
24 slots, 260mm long
Applicator Strips 3.5 x 2
43 slots, 240mm long
Applicator Strip Kit
One of each: 42914, 42915, 42989, 42899
Sample Application Pieces
10 x 5mm
• GMP/GLP conformity - each strip has its individual lot number
6 strip
42915.01
IPG Strips
3 strips
42989.01
3 strips
42899.01
4 strips
42937.01
200 pieces
42880.01
IPG BlueStrip pH 3 - 10
IPG BlueStrip pH 3 - 10
IPG BlueStrip pH 3 - 10
IPG BlueStrip pH 3 - 10 NL*
IPG BlueStrip pH 3 - 10 NL*
IPG BlueStrip pH 3 - 10 NL*
IPG BlueStrip pH 4 - 7
IPG BlueStrip pH 4 - 7
IPG BlueStrip pH 4 - 7
IPG BlueStrip pH 6 - 10
IPG BlueStrip pH 6 - 10
IPG BlueStrip pH 6 - 10
122
7cm
18cm
24cm
7cm
18cm
24cm
7cm
18cm
24cm
7cm
18cm
24cm
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
12 strips
43001.01
43011.01
43021.01
43002.01
43012.01
43022.01
43003.01
43013.01
43023.01
43004.01
43014.01
43024.01
100 pieces
42988.01
* NL Non-Linear Gradient
100 pieces
42987.03
100 pieces
42972.03
Rehydration tray for IPG Strips
To rehydrate up to 12 IPG strips in lengths up to 24cm. The
rehydration tray maintains its shape and is resistant against
chemicals normally used to rehydrate IPG strips.
Rehydration Tray
50ml
42984.03
Cathode Fluid
0.22g L-arginine, 0.18g L-lysine, 6.0ml ethylenediamine in water.
Cathode Fluid 10
• Reliability - accurate casting procedures ensures lot-to-lot
reproducibility of pH gradients
42914.01
Anode Fluid
0.17g L-aspartic acid / 0.18g L-glutamic acid in water.
Anode Fluid 3,
• Consistent performance - 12 strips per package, all derived
from the same production lot
6 strips
Electrode Wicks
Available in three sizes: Standard - 120 x 6 x 1mm,
Long - 240 x 6 x 1mm and Extra - 300 x 6 x 1mm.
Electrode Wicks, Standard Size
120 x 6 x 1mm
Electrode Wicks, Long Size
240 x 6 x 1mm
Electrode Wicks, Extra Size
300 x 6 x 1mm
IPG BlueStrips are dried gel strips, incorporating an
immobilised pH gradient, used in high resolution 2-D
gel electrophoresis of proteins.
50ml
42986.03
Each
43091.01
VERTICAL PRECAST GELS FOR MINI VERTICAL
ELECTROPHORESIS.
A novel range of precast gels, ready-to-use and cast
from acrylamide-bis in unbreakable safety cassettes.
The SERVAGel™ gives premium resolution and superb
band sharpness. The 10 x 10cm format is compatible
with many vertical electrophoresis units, particularly
the TV50 and TV100 units. SERVAGel™ are packed
single and vacuum sealed.
BENEFITS INCLUDE
• Easy and safe to operate, no leakage
• Short set-up times, gels are ready to use
• Unbreakable, recyclable plastic cassette
• Minimum health risk (polymerised acrylamide, non-toxic)
• For gradient gels, please inquire
SERVAGel™ TG 8 - Vertical Tris-Glycine Gel 8%
Storage Temperature +20°C to +80°C.
The SERVAGel™ TG 8 can be operated in the presence or absence
of SDS (SDS-PAGE, native PAGE). The separation range is from
60 to 200KDa. Shelf life is 3 months.
SERVAGel™ TG 8 - Tris-Glycine Gel 8%
SERVAGel™ TG 8 - Tris-Glycine Gel 8%
SERVAGel™ TG 8 - Tris-Glycine Gel 8%
2 gels
6 gels
10 gels
43208.03
43208.02
43208.01
SERVAGel™ TG 10 - Vertical Tris-Glycine Gel 10%
Storage Temperature +20°C to +80°C.
The SERVAGelTM TG 10 can be operated in the presence or absence
of SDS (SDS-PAGE, native PAGE). The separation range is from 25
to 200KDa.
Shelf life is 3 months.
SERVAGel™ TG 10 - Tris-Glycine Gel 10%
SERVAGel™ TG 10 - Tris-Glycine Gel 10%
SERVAGel™ TG 10 - Tris-Glycine Gel 10%
2 gels
6 gels
10 gels
43210.03
43210.02
43210.01
SERVAGel™ TG 12 - Vertical Tris-Glycine Gel 12%
Storage Temperature +20°C to +80°C.
The SERVAGel™ TG 12 can be operated in the presence or absence
of SDS (SDS-PAGE, native PAGE). The separation range is from 15
to 100KDa.
Shelf life is 3 months.
SERVAGel™ TG 12 - Tris-Glycine Gel 12%
SERVAGel™ TG 12 - Tris-Glycine Gel 12%
SERVAGel™ TG 12 - Tris-Glycine Gel 12%
2 gels
6 gels
10 gels
43212.03
43212.02
43212.01
SERVAGel™ TG 14 - Vertical Tris-Glycine Gel 14%
Storage Temperature +20°C to +80°C.
The SERVAGel™ TG 14 can be operated in the presence or absence
of SDS (SDS-PAGE, native PAGE). The separation range is from 10
to 90KDa.
Shelf life is 3 months.
SERVAGel™ TG 14 - Tris-Glycine Gel 14%
SERVAGel™ TG 14 - Tris-Glycine Gel 14%
SERVAGel™ TG 14 - Tris-Glycine Gel 14%
2 gels
6 gels
10 gels
43214.03
43214.02
43214.01
SERVAGel™ TG 16 - Vertical Tris-Glycine Gel 16%
Storage Temperature +20°C to +80°C.
The SERVAGel™ TG 16 can be operated in the presence or absence
of SDS (SDS-PAGE, native PAGE). The separation range is from 7
to 80KDa.
Shelf life is 3 months.
SERVAGel™ TG 16 - Tris-Glycine Gel 16%
SERVAGel™ TG 16 - Tris-Glycine Gel 16%
SERVAGel™ TG 16 - Tris-Glycine Gel 16%
2 gels
6 gels
10 gels
43216.03
43216.02
43216.01
SERVAGel™ Neutral pH 7.4
Storage Temperature +20°C to +80°C.
The SERVAGel™ Neutral pH 7.4 can be operated with various
buffer systems, such as Tris-Glycine, MOPS-Tris and Tris-Tricine.
The separation range is from 6.5 to 200KDa.
Shelf life is 3 months.
SERVAGel™ Neutral pH 7.4
SERVAGel™ Neutral pH 7.4
SERVAGel™ Neutral pH 7.4
2 gels
6 gels
10 gels
43220.03
43220.02
43220.01
123
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
Precast PAGE Gels
Reagents
Blotting Buffers & Membranes
GEL BLOTTING PAPER - FN100
Gel blotting paper with an extremely smooth surface and a 0.35mm
thickness. Made from the purest naturally occurring raw materials,
offering the maximum degree of absorption and α cellulose content.
Fast running and high absorption capacity; ideal combination of
chromatography and gel blotting paper. The ideal general purpose
blotting paper for Southern, Northern and Western blotting, gel
lifting, sequencing, buffer wicking and semi-dry blotting.
BENEFITS INCLUDE
• Superior uniformity across the entire contact area in the
blotting transfer system
• Absorption and improvement in the transport of transfer buffer
after capillary and semi-dry blotting
• Double-sided cover of gel and transfer membrane in the
blotting tank after conventional electroblotting
• To prevent direct contact between the blotting membrane and
porous cover plate of the vacuum chamber for dot/slot blotting
of RNA and DNA
200 x 200mm
100 Sheets
FN100
Towbin Buffer, 10x, for Western Blotting
Supplied as a 10x concentrate: (0.25M Tris and 1.92M Glycine in
aqueous solution.
Working buffer: dilute 100ml of 10x concentrate with 200ml of
methanol and 700ml distilled water.
Towbin Buffer, 10x
1L
42558.02
Semi-Dry Blotting Buffer Kit
Supplied as a ready-to-use kit, comprising of 3 components for
Western blotting in the semi-dry blotting units.
Buffer 1. (Conc. Anode buffer): 0.3M Tris and 20% Methanol in
aqueous solution.
Buffer 2. (Diluted Anode buffer): 0.03M Tris and 20% Methanol in
aqueous solution.
Buffer 3. (Cathode buffer): 0.25M Tris/HCl (pH 9.4), 0.04M 6Aminocaproic acid and 20% Methanol in aqueous
solution.
Semi-Dry Blotting Buffer Kit
2 x 500ml
42559.01
BLOTTING MEMBRANES
Scie-Plas offers 5 different types of blotting membrane for use with all
transfer techniques. Each membrane is available in 10 x 10cm and 20 x
20cm formats and in 0.2 and 0.45µm pore sizes for the binding of low
(<20kDa) and high (>20kDa) molecular weight proteins and nucleic acids.
Nitrocellulose Blotting Membranes
Nitrocellulose membranes are the most popular membranes for Western
Southern and Northern blotting. The membranes bind both proteins and
nucleic acids. Nitrocellulose exhibits high binding capacity and has low
background.
0.02µm pore size, 100mm x 100mm
0.02µm pore size, 200mm x 200mm
0.45µm pore size, 100mm x 100mm
0.45µm pore size, 200mm x 200mm
124
25 Sheets
25 Sheets
25 Sheets
25 Sheets
EB-MEM-NC20
EB-MEM-NC20L
EB-MEM-NC45
EB-MEM-NC45L
Supported Nitrocellulose Blotting Membranes
As per nitrocellulose but more robust for frequent handling.
0.02µm pore size, 100mm x 100mm
0.02µm pore size, 200mm x 200mm
0.45µm pore size, 100mm x 100mm
0.45µm pore size, 200mm x 200mm
25 Sheets
25 Sheets
25 Sheets
25 Sheets
EB-MEM-SNC20
EB-MEM-SNC20L
EB-MEM-SNC45
EB-MEM-SNC45L
Polyvinylidene Fluoride (PVDF) Blotting Membranes
A hydrophobic membrane with much higher protein-binding capacity than
nitrocellulose.
0.02µm pore size, 100mm x 100mm
0.02µm pore size, 200mm x 200mm
0.45µm pore size, 100mm x 100mm
0.45µm pore size, 200mm x 200mm
25 Sheets
25 Sheets
25 Sheets
25 Sheets
EB-MEM-PDVF20
EB-MEM-PDVF20L
EB-MEM-PDVF45
EB-MEM-PDVF45L
Supported Nylon-66 Blotting Membranes
A more versatile membrane, with reduced background to increase
sensitivity, for the binding of nucleic acids after semi-dry blotting, capillary
Southern and Northern transfer; more robust for frequent handling.
0.02µm pore size, 100mm x 100mm
0.02µm pore size, 200mm x 200mm
0.45µm pore size, 100mm x 100mm
0.45µm pore size, 200mm x 200mm
25 Sheets
25 Sheets
25 Sheets
25 Sheets
EB-MEM-SN20
EB-MEM-SN20L
EB-MEM-SN45
EB-MEM-SN45L
Positively Charged Supported Nylon-66 Blotting Membranes
Higher binding affinity than supported Nylon-66 for negatively charged
molecules such as nucleic acids during Southern and Northern transfer;
more robust for frequent handling.
0.02µm pore size, 100mm x 100mm
0.02µm pore size, 200mm x 200mm
0.45µm pore size, 100mm x 100mm
0.45µm pore size, 200mm x 200mm
25 Sheets
25 Sheets
25 Sheets
25 Sheets
EB-MEM-SN20
EB-MEM-SN20L
EB-MEM-SN45
EB-MEM-SN45L
t: +44 (0)1223 427888 f: +44 (0)1223 420164 e: [email protected] i: www.scie-plas.com
125
Terms and Conditions
Scie-Plas Limited Terms of Trading
4
Payment terms
Buyer: The person, firm or company who purchases Goods from the
Company
4.1
The Buyer is to pay the Company cash (or otherwise in cleared funds
such as by credit card) on delivery unless the Buyer has an approved
credit account.
4.2
If the Buyer has an approved UK business credit account, payment is
due 30 days from the invoice date, unless otherwise agreed in writing.
Company: Scie-Plas (UK) Limited
Contract: any Contract between the Company and the Buyer for the sale
and purchase of the Goods, incorporating these conditions
4.3
Time for payment shall be of the essence.
Goods: any Goods agreed in the Contract to be supplied to the Buyer by the
Company (including any part or parts of them).
4.4
No payment shall be deemed to have been received until the Company
has received cleared funds.
Company Warehouse: the warehouse of the Company at which deliveries
will be effected pursuant to Clause 6.1, the location of which has been
notified to the Buyer.
4.5
If the Buyer fail to pay the Company in full on the due date:
4.5.1 the Company may suspend or cancel future deliveries;
1
Price
4.5.2 the Company may cancel any discount offered to the Buyer;
1.1
The price quoted excludes VAT (unless otherwise stated). VAT will be
charged at the rate applying at the time of delivery.
1.2
The Company’s quotations lapse after thirty days (unless otherwise
agreed).
4.5.3 the Buyer must pay the Company interest at the rate equivalent
to that set for the purposes of section 6 of the Late Payment of
Commercial Debts (Interest) Act 1998;
1.3
The price quoted excludes delivery (unless otherwise stated).
1.4
Unless otherwise stated, the price quoted is an illustrative estimate
only and the price charged will be the Company’s price current at the
time of delivery.
1.5
Rates of tax and duties on the Goods will be those applying at the time
of delivery.
1.6
At any time before delivery the Company may adjust the price to
reflect any increase in the costs of supplying the Goods.
2
Delivery
2.1
Any dates specified by the Company for delivery of the Goods are
intended to be an estimate and time for delivery shall not be made of
the essence by notice. If no dates are so specified, delivery shall be
within a reasonable time.
2.2
If the Company fails to deliver within a reasonable time, the Buyer may
(in writing) cancel the Contract, however:
2.2.1 the Buyer may not cancel if the Company receives notice after
the Goods have been despatched; and
2.2.2 if the Buyer cancels the Contract, the Buyer can have no further
claim against the Company under the Contract.
2.3
2.4
If the Buyer accepts delivery of the Goods after the estimated delivery
time, it will be on the basis that the Buyer has no claim against the
Company for delay (including indirect or consequential loss, or
increase in the price of the Goods).
(i)
calculated (on a daily basis) from the date of the
Company’s invoice until payment;
(ii)
compounded on the first day of each calendar month; and
(iii)
before and after any judgement (unless the court orders
otherwise).
4.6
If the Buyer has an approved business credit account the Company
may withdraw it or reduce the credit limit or bring forward the Buyer’s
due date for payment.
4.7
The Company may take any of those actions in 4.6 at any time and
without notice.
4.8
The Buyer shall make all payments due under the Contract in full
without any deduction whether by way of set-off, counterclaim,
discount, abatement or otherwise unless the Buyer has a valid court
order requiring an amount equal to such deduction to be paid by the
Company to the Buyer.
4.9
While the Buyer owes money to the Company, the Company has a
right to keep any property the Company may hold of the Buyer’s until
the money is paid to the Company in full.
4.10 The Buyer must indemnify the Company in full and hold the Company
harmless from all expenses and liabilities the Company may incur
(directly or indirectly and including legal costs on a full indemnity
basis) following any breach by the Buyer of any of their obligations
under these terms.
5
Title
5.1
Ownership of the Goods shall not pass to the Buyer until the Company
has received in full (in cash or cleared funds) all sums due to it in
respect of:
The Company may deliver the Goods by separate instalments. Each
separate instalment shall be invoiced and paid for in accordance with
the provisions of the Contract.
5.1.1 the Goods; and
5.1.2 all other sums which are or which become due to the Company
from the Buyer on any account.
2.5
Each instalment shall be a separate Contract and no cancellation or
termination of any one Contract relating to an instalment shall entitle
the Buyer to repudiate or cancel any other Contract or instalment.
3
Delivery and safety
Until ownership of the Goods has passed to the Buyer, the Buyer
shall:
3.1
The Company may decline to deliver if:
5.2.1 hold the Goods on a fiduciary basis as the Company's bailee;
3.1.1 it believes that it would be unsafe, unlawful or unreasonably
difficult to do so; or
5.2.2 store the Goods (at no cost to the Company) separately from all
other Goods of the Buyer or any third party in such a way that
they remain readily identifiable as the Company's property;
3.1.2 the premises (or the access to them) are unsuitable for the
Company’s vehicle.
3.2
126
Any liability of the Company for non-delivery of the Goods shall be
limited to replacing the Goods within a reasonable time or issuing a
credit note at the pro rata Contract rate against any invoice raised for
such Goods.
5.2
5.2.3 not destroy, deface or obscure any identifying mark or
packaging on or relating to the Goods; and
5.2.4 maintain the Goods in satisfactory condition and keep them
insured on the Company's behalf for their full price against all
risks to the reasonable satisfaction of the Company. On request
the Buyer shall produce the policy of insurance to the Company.
The Buyer may resell the Goods before ownership has passed to it
solely on the following conditions:
5.3.1 any sale shall be effected in the ordinary course of the Buyer's
business at full market value; and
5.3.2 any such sale shall be a sale of the Company's property on the
Buyer's own behalf and the Buyer shall deal as principal when
making such a sale.
5.4
The Buyer's right to possession of the Goods shall terminate
immediately if:
5.4.1 the Buyer has a bankruptcy order made against him or makes
an arrangement or composition with his creditors, or otherwise
takes the benefit of any statutory provision for the time being in
force for the relief of insolvent debtors, or (being a body
corporate) convenes a meeting of creditors (whether formal or
informal), or enters into liquidation (whether voluntary or
compulsory) except a solvent voluntary liquidation for the
purpose only of reconstruction or amalgamation, or has a
receiver and/or manager, administrator or administrative
receiver appointed of its undertaking or any part thereof, or
documents are filed with the court for the appointment of an
administrator of the Buyer or notice of intention to appoint an
administrator is given by the Buyer or its directors or by a
qualifying floating charge holder (as defined in paragraph 14 of
Schedule B1 to the Insolvency Act 1986), or a resolution is
passed or a petition presented to any court for the winding-up
of the Buyer or for the granting of an administration order in
respect of the Buyer, or any proceedings are commenced
relating to the insolvency or possible insolvency of the Buyer; or
5.4.2 the Buyer suffers or allows any execution, whether legal or
equitable, to be levied on his/its property or obtained against
him/it, or fails to observe or perform any of his/its obligations
under the Contract or any other Contract between the
Company and the Buyer, or is unable to pay its debts within the
meaning of section 123 of the Insolvency Act 1986 or the Buyer
ceases to trade; or
5.4.3 the Buyer encumbers or in any way changes any of the Goods.
5.5
The Company shall be entitled to recover payment for the Goods
notwithstanding that ownership of any of the Goods has not passed
from the Company.
5.6
The Buyer grants the Company, its agents and employees an
irrevocable licence at any time to enter any premises where the
Goods are or may be stored in order to inspect them, or, where the
Buyer's right to possession has terminated, to recover them.
5.7
Where the Company is unable to determine whether any Goods are
the Goods in respect of which the Buyer's right to possession has
terminated, the Buyer shall be deemed to have sold all Goods of the
kind sold by the Company to the Buyer in the order in which they were
invoiced to the Buyer.
5.8
On termination of the Contract, howsoever caused, the Company's
(but not the Buyer's) rights contained in this condition 5 shall remain
in effect.
5.9
The Buyer is not the Company’s agent and has not authority to make
any Contract on the Company’s behalf or in the Company’s name.
6
Risk
6.1
Delivery shall be FCA (Incoterms 2000) at the Company Warehouse
and the Goods are at the Buyer’s risk from the time of delivery.
6.2
The Buyer must inspect the Goods on delivery, which shall be at the
Company Warehouse in accordance with Clause 6.1. If any Goods are
damaged, the Buyer must write to tell the Company within seven
working days of delivery. The Buyer must give the Company a
reasonable opportunity to inspect the damaged Goods.
7
Warranties
7.1
The Company warrants that the Goods:
7.1.1 be of satisfactory quality within the meaning of the Sale of
Goods Act 1979;
7.1.2 comply with their description on the Company’s delivery note;
and
7.1.3 are free from material defect at the time of delivery (as long as
the Buyer complies with clause 6.3).
7.2
The Company gives no other warranty (and excludes any warranty,
term or condition that would otherwise be implied) as to the quality
of the Goods or their fitness for any purpose.
7.3
If the Buyer believes that the Company has delivered Goods which are
defective in material or workmanship, the Buyer must:
7.3.1 inform the Company (in writing), with full details, as soon as
possible; and
7.3.2 allow the Company to investigate (the Company may need
access to Buyer’s premises and product samples).
7.4
if the Goods are found to be defective in material or workmanship
(following the Company’s investigations), and the Buyer has complied
with those conditions (in clause 7.3) in full, the Company will (at it’s
option) replace the Goods or refund the price.
7.5
The Company’s entire liability and obligation, and the Buyer’s
exclusive remedy with respect to any breach of warranties under
Clause 7.1, shall be limited to replacing the Goods in accordance with
Clause 7.4. Except for such replacement obligation, the Company
shall not be liable for any defect, damage or loss with respect to any
of the Goods, even if the same if caused by the negligence or other
fault of the Company. Nothing in this clause or in these terms shall
limit the Company’s liability for personal injury or death caused by its
negligence.
127
t: +44 (0)1926 814093 f: +44 (0)1926 813975 e: [email protected] i: www.scie-plas.com
5.3
SCIE-PLAS
Scie-Plas Ltd
22 Cambridge Science Park
Milton Road
Cambridge
CB4 0FJ
United Kingdom
T: +44 (0) 1223 427888
F: +44 (0) 1223 420164
e: [email protected]
www.scie-plas.com
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