BioTek Microplate Instrumentation

BioTek Microplate Instrumentation
BioTek Instruments • Catalog 2015
BioTek Microplate Instrumentation
BioTek Instruments, Inc., headquartered in Winooski, VT, USA, is a
worldwide leader in the design, manufacture, and sale of microplate
instrumentation and software. BioTek instrumentation is used to aid in
the advancement of life science research, facilitate the drug discovery
process, provide rapid and cost-effective industrial analysis and to enable
sensitive and accurate quantification of a wide range of molecules across
diverse applications.
Our company-wide commitment to quality and value is backed by superior
customer care, technical service centers, scientific application experts,
and a knowledgeable sales force. Our commitment and focus helps to
ensure your processes will be rapid, efficient and successful.
This catalog provides an overview of our complete line of microplate
instrumentation, and features Cytation, the first instrument to combine
automated digital microscopy and conventional microplate detection.
For more detailed information and specifications, visit our web site
at www.biotek.com.
BioTek is headquartered in Vermont, USA with global sales, service and distribution support. BioTek
is ISO9001/ISO13485 Certified, an FDA Registered Medical Device Manufacturer, and has appropriate
products in compliance with the EU In Vitro Diagnostic Directive (IVD). Regulatory compliance is ensured
with both validation (IQ/OQ/PQ) and FDA 21 CFR Part 11 tools.
u Imaging & Microscopy ...................................................4
NEW Cytation™ ........................................................................... 6
Gen5™ Data Analysis Software .......................................... 8
u Detection ........................................................................10
Hybrid Technology Multi-Mode Microplate Readers
Synergy™ Neo ................................................................... 12
Synergy H1......................................................................... 14
Multi-Mode Microplate Readers
Synergy 2 ..........................................................................16
NEW Synergy HTX .....................................................................18
Multi-Mode Reader Comparison Chart .............................20
Fluorescence Microplate Readers
FLx800™ .............................................................................22
Absorbance Microplate Readers
PowerWave™ HT.................................................................23
NEW Epoch™ 2 ........................................................................... 24
Epoch ................................................................................ 26
ELx808™ .............................................................................28
ELx800™ .............................................................................29
Absorbance Reader Comparison Chart ............................30
Gen5 Data Analysis Software ............................................32
Take3™ Micro-Volume Plate ..............................................34
Reader Accessories ...........................................................35
u Liquid Handling .............................................................. 36
Combination Washer Dispenser
EL406™ ...............................................................................38
Microplate Washers
405™ Touch .......................................................................40
ELx405™ Select Deep Well ...............................................40
ELx50™ ...............................................................................42
Washer Comparison Chart ................................................ 44
Microplate Dispensers
NEW MultiFlo™ FX ......................................................................46
MicroFill™ ...........................................................................48
Dispenser Comparison Chart ............................................ 50
Liquid Handling Control Software ....................................52
Washer & Dispenser Accessories ...................................... 53
Pipetting Systems
Precision™ ..........................................................................54
u Robotics ..........................................................................56
BioStack™ ...........................................................................58
u Service & Support ..........................................................60
Compliance ....................................................................... 62
Applications Support ........................................................ 64
Global Service and Support ..............................................65
Decades of Innovation ............................................................. 66
Contact Information ................................................................. 69
4
Imaging & Microscopy n Overview
Imaging &
Microscopy
The fusion of automated digital widefield fluorescent
microscopy and multi-mode microplate detection in
the new Cytation Cell Imaging Multi-Mode Readers
offers
life
science researchers a unique and
powerful tool. Whether studying fixed or live cells,
tissues or whole organisms, Cytation’s automated
fluorescence microscopy and detection of a wide
array of fluorescent probes provides quantitative
phenotypic data from images. These cellular images
provide rich visualization and multi-parametric
information concerning many biological processes.
This unique, patent pending design results in
workflow efficiency and a reduction in data analysis
and storage requirements, making Cytation the
ideal instrument as a standalone automated imager
or in combination with microplate reading as a
total system.
www.biotek.com
5
Cytation™ Cell Imaging Multi-Mode Reader
Cytation™ is a uniquely
integrated, configurable system
that combines automated
digital widefield microscopy
with conventional multi-mode
microplate detection to provide
phenotypic cellular information
and well-based quantitative data.
With up to 60x magnification,
the microscopy module provides
high-quality cellular and subcellular visualization. The multimode detection module provides
high quality quantitative and
qualitative data in all detection
modes. All controlled with
Gen5™ software, specifically
designed for uncomplicated
processing of even the most
complex assays
Powerful Automated Digital
Microscopy
The Cytation family includes
Cytation 5 and Cytation 3.
Each is available in multiple
upgradable configurations; from
basic microscopy to complex
image collection and analysis in
fluorescence, brightfield, H&E
and phase contrast, Cytation
offers critical methods like singleand multi-color, time lapse,
montage and z-stacking. Gen5
software offers unique features
like user-trained autofocus and
available joystick control for truly
automated imaging with the
highest quality results. Powerful
image processing like image
stitching, z-projection and digital
phase contrast, are all available in
this cost effective system.
Live Cell Assays
To create the ideal environment for
live cell assays, Cytation systems
include 4-Zone™ incubation up
to 65 °C and a gas controller to
monitor and control CO2 and O2
levels in the system. Linear, orbital
and double orbital shaking help
keep cells gently agitated or well
suspended to optimize many cell
based assay protocols.
Typical Applications:
XX2D
and 3D cell imaging and analysis
XXCell
proliferation studies
XXCytotoxicity
XXBiomarker
XXDrug
discovery
XXGenetic
XXDrug
quantification
analysis
absorption and metabolism
XXBiologics
drug discovery and development
XXEnvironmental
XXFood
6
testing
safety
XXNucleic
acid quantification
XXProtein
quantification
Imaging & Microscopy n Cytation
Hybrid Technology
BioTek’s patented Hybrid
Technology, available with the
multi-mode detection modules
for Cytation, combines high
performance filters with variable
bandwidth monochromators,
providing convenience, versatility
and excellent performance.
Luminescence, UV-Vis absorbance,
time-resolved fluorescence,
fluorescence polarization and
AlphaScreen®/AlphaLISA®
measurements read modes greatly
increase the application range of
the system.
Specifications
General
Cytation 5
Cytation 5
Detection modes
Cytation 3
AlphaScreen/AlphaLISA
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Absorbance
Luminescence
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Absorbance
Luminescence
Cytation 3
Monochromator
bandwidth
Variable from 9 nm to
50 nm in 1 nm increments
Detection system
Two PMT detectors: one for monochromator system, one
for filter system
Dynamic range
7 decades
Fixed, 16 nm
5 decades
Luminescence
Microplate types
6- to 384-well plates
Other labware
Microscope slides, Petri dishes, cell culture flasks (T25),
compatible with Take3™ Micro-Volume Plates
Temperature
control
4-Zone incubation to
65°C with Condensation
Control; ±0.2 °C at 37 °C
Shaking
Linear, orbital, double orbital
Software
Gen5 Data Analysis Software
Automation
BioStack and 3rd party automation compatible
CO2 and O2
control
Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution:
±0.1% (CO2 and O2) Stability: ±0.2% at 5% CO2; ±0.2%
at 1% O2
Models for both CO2 and O2 or CO2 only are available
Light source
Xenon flash - fluorescence and absorbance
100 mW laser - AlphaScreen/AlphaLISA
4-Zone incubation to 45 °C
with Condensation Control;
±0.2 °C at 37 °C
™
Sensitivity
Monochromator system: 20 amol ATP (flash)
Filter system: 10 amol ATP (flash) 100 amol ATP (glow)
Wavelength range
300 - 700 nm
Dynamic range
>6 decades
Fluorescence Polarization
Sensitivity
1.2 mP standard deviation at 1 nM fluorescein
Wavelength range
280 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Sensitivity
Europium 40 fM with filters (4 amol/well in 384-well plate)
Europium 1200 fM with monos (120 amol/well in 384-well plate).
Wavelength range
Monos: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Alpha Detection
Imaging System
Camera
16-bit gray scale, Sony CCD, 1.1 megapixel
Light source
680 nm laser, 100 mW
n/a
Imaging mode
Fluorescence
Brightfield
Phase Contrast
H&E Imaging
Wavelength
selection
Filter (top only)
n/a
Sensitivity
100 amol LCK peptide
(384-well low volume plate)
n/a
Fluorescence
Brightfield
Imaging method
Single color, Multi-color, Montage, Time Lapse,
Z-stacking
Image processing
Z-Projection, Digital Phase Contrast, Stitching
Positional controls
Joystick control
Software control
Automated
functions
User-trained autofocus, autofocus, autoexposure,
auto-LED intensity
Imaging filter cube
capacity
4 onboard, user-replaceable filter cubes
Imaging filter cubes
available
DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,
Acridine Orange (ACR OR), CFP-YFP FRET, propidium
Iodide, chlorophyll, phycoerythrin
Number
2 syringe pumps
Imaging LED
cubes available
365 nm, 405 nm, 465 nm, 590 nm, 523 nm, 505 nm,
623 nm, 740 nm
Supported
detection modes
All modes
Objective capacity
6 user-replaceable
objectives
Dispense volume
5 - 1000 µL in 1 µL increment
Dead volume
1 mL, 100 µL with back flush
Plate geometry
6- to 384-well plates, Petri dishes
Dispense precision
≤2% at 50-200 µL
Dispense accuracy
±1 µL or 2%
Available objectives
2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives
4x, 10x, 20x, 40x
Image collection
rate
Software control
2 user-replaceable
objectives
n/a
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes
Fluorescence Intensity
Sensitivity
Monochromators:
Top: fluorescein 2.5 pM (0.25 fmol/well 384-well plate)
Bottom: fluorescein 4 pM (0.4 fmol/well 384-well plate)
Filters/mirrors:
Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)
Wavelength
selection
Quadruple monochromators (top/bottom) and deep
blocking bandpass filters/dichroic mirrors (top)
Wavelength range
Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Specifications are subject to change. Performance values represent the average
observed factory test values
Absorbance
Wavelength
selection
Monochromator
Wavelength range
230 - 999 nm, 1 nm increment
Bandwidth
4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic range
0 - 4.0 OD
Resolution
0.0001 OD
Reagent Injectors
Physical Characteristics
Power
250 Watts max.
Dimensions
20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight
80 lbs (36.3 Kg)
130 Watts max.
Regulatory
Regulatory
CE and TUV marked. RoHS Compliant. Models for In Vitro
Diagnostic use are available.
www.biotek.com
7
Gen5™ Data Analysis Software for Cytation™
Gen5™ can automate the entire
imaging process: all the steps
of image acquisition are fully
automated including X, Y and Z
movements, focus, LED intensity
and camera exposure. Image
analyses like cell counting and
subpopulation analysis happen
on the fly as images are acquired;
Gen5’s data analysis tools: kinetic
analysis, curve fitting, EC50,
potency calculation and others
can then be applied to image
analysis results. With Gen5 you
can go from samples to final
results in one click.
Imaging Made Easy
Gen5 was designed for users
without prior automated
microscopy experience. Image
capture and acquisition is done
through a simple interface that
includes auto focus and auto
exposure algorithms. Image
analysis can be as simple as a one
click process with visual feedback
to fine-tune analysis parameters.
Automatic Cell Counting
Gen5 can automatically count
cell nuclei in samples. In a 96- or
384-well microplate, this means
performing a count on tens of
thousands of cells in a matter of
minutes. This makes Cytation
a very powerful tool for cellular
assays where multiple conditions
need to be tested and changes
in cell population need to be
monitored.
Sub-Population Analysis
Gen5 can sort cells by features
such as intensity, or morphology
(size, perimeter, circularity). This
enables applications such as
transfection efficiency, nuclear
translocation or cell cycle assays
where multiple cell sub-populations
are present in the samples.
Hit Picking
Gen5 can be programmed to
quickly scan the plate with the
standard plate reader optics and
hit-pick the wells that meet a
defined threshold for imaging.
This saves valuable time, reduces
data collection, analysis and
storage requirements – ultimately
reducing costs.
Universal Image Format
Gen5 works with a universal
TIF image format. TIF images
acquired on microscopes can be
imported into Gen5 for analysis.
On the other hand, exported
Gen5 images are compatible with
a broad range of image analysis
software including Image J or
Cell Profiler if custom analysis
is required.
Key Features:
Powerful instrument control
XXReliable autofocus methods including unique
user-trained autofocus
HCT 116 tumoroids at 20x. Z-stacked image
XXAutomatic
camera gain, exposure and LED
intensity settings
XXEnd-point,
montage, Z-stack and time-lapse
read modes
Image pre-processing tools
XXBuilt-in
hot-pixel correction
XXAutomated
image pre-processing (flattening,
smoothing, background correction)
XXImage
stitching, Z-projection
XXDigital
phase contrast algorithm
Image and data analysis tools
XXAutomatic
XXPowerful
XXImage
cell-counting and confluence
sub-population analysis
statistics
XXFull
data analysis software (EC50, standard
curves, kinetic analysis and more)
Liver cirrhosis, 4x, stitched. Color brightfield
8
Imaging & Microscopy n Gen5 Software
Zebrafish at 4x. Texas Red (blood cells)
& GFP (vasculature)
BPAE cells at 20x. Texas Red, Phalloidin & GFP
Euglena gracilis at 60x. Brightfield
& chlorophyll (Red)
Lillium anther at 20x. Phase contrast, GFP
& Texas Red
HT-1080 cells, migration assay at 2.5x, 1 hr to 12 hr run
www.biotek.com
9
10
Detection n Overview
Detection
BioTek offers an extensive range of microplate
readers, from the Synergy™ Neo Multi-Mode
Microplate Reader to the ELx800™, a basic ELISA
reader used in tens of thousands of laboratories
around the globe. Included in the BioTek reader
product range are Hybrid readers, multi-mode
readers, fluorometers, luminometers and a variety of
both monochromator-based spectrophotometers
and filter-based absorbance readers.
For assays like nucleic acid and protein quantification,
where very small sample size is critical, BioTek’s
Take3™ Micro-Volume Plate offers the ability to
measure multiple samples as small as 2 µL in
monochromator-based multi-mode and absorbance
readers. Most BioTek microplate readers come with
the powerful Gen5™ Data Analysis Software and
are compatible with BioStack™ and many thirdparty automation products, to provide increased
throughput and unattended operation.
www.biotek.com
11
Synergy™ Neo Hybrid Multi-Mode Microplate Reader
Synergy™ Neo is a multi-mode
microplate reader designed
specifically for today’s screening
and core laboratories. Neo has all
the features expected in an HTS
screening instrument, including
multiple parallel detectors for
ultra-fast measurements, a
dedicated filter-based system
for live cell assays, and exquisite
performance.
Hybrid Technology™ Optical
System
High Throughput
Synergy Neo meets today’s
diverse assay requirements by
incorporating features like a
powerful 100 mW laser-based
excitation source for Alpha-based
assays (AlphaScreen®, AlphaLISA®,
SureFire®). The Alpha capability
of Neo provides speed and
sensitivity even for low-signal cellbased assays.
Synergy Neo can read a 96-well
plate in 6 seconds and a 384well plate in 11 seconds. With an
exchange time of about 6 seconds
per plate, its optional integrated
plate stacker makes this the fastest
combination on the market,
allowing walk-away automation of
short- or long-term assays.
At Synergy Neo’s core is BioTek’s
patented Hybrid design that
incorporates monochromatorbased and filter-based systems
for the utmost flexibility and
performance in HTS, research and
drug discovery laboratories.
Assay Versatility
Other features, such as advanced
temperature control to 65°C,
linear, orbital and double
orbital shaking round out the
powerhouse of functionality and
performance that defines the Neo.
Proven performance in HTRF®,
fluorescence polarization, timeresolved fluorescence and TRFRET applications makes Neo and
Gen5™ software an award-winning
system.
Designed for Automation
Ease of use and user confidence
are key to the design of Synergy
Neo’s unique filter cubes that are
barcode labeled for positive filter
ID – limiting the possibility of errors
and streamlining the workflow. An
available camera-based barcode
reader can scan both 1D and 2D
barcode-labeled plates.
Typical Applications:
XXHTS
screening
XXDrug
absorption and metabolism
XXBiologics
XXDrug
XXCell
drug discovery and development
discovery
proliferation
XXCytotoxicity
XXBiomarker
XXGenetic
quantification
analysis
XXEnvironmental
XXFood
12
testing
safety
XXNucleic
acid quantification
XXProtein
quantification
Detection n Synergy Neo
Synergy Neo shown with optional
high-speed microplate stacker
Specifications
Luminescence
General
Wavelength
selection
Hybrid Technology (patented)
Quadruple Monochromators and Filter Cubes
Detection mode
Fluorescence, Time-Resolved Fluorescence, Fluorescence
Polarization, AlphaScreen®/AlphaLISA®, Luminescence,
UV-Visible Absorbance
Read mode
End point, kinetic, spectral scanning, well-area scanning
Microplate types
1- to 1536-well plates
Other labware
Take3™ Micro-Volume Plates
Temperature
control
™
Sensitivity
5 amol ATP typical (384-well low volume plate)
Wavelength range
300 - 700 nm
Dynamic range
>6 decades
Fluorescence Polarization
Light source
Xenon flash
Sensitivity
1 mP standard deviation at 1 nM fluorescein
(384-well low volume plate)
4-Zone™ incubation to 65 °C; variation ±0.2 °C at 37 °C
with Condensation Control™
Wavelength
selection
Filter cubes (top/bottom)
Shaking
Linear, orbital, double orbital
Wavelength range
280 - 850 nm
Automation
BioStack™ and 3rd party automation compatible
Read height
Auto Z, 0.1 mm steps, top/bottom (filters), top (mono)
Light source
Xenon flash
Kinetic speed
96 well: 6 seconds
384 well: 11 seconds
1536 well: 25 seconds
Sensitivity
Europium 40 fM (384-well low volume plate)
Wavelength
selection
Double grating monochromator (top/bottom)
Filter cubes (top/bottom)
Wavelength range
Monochromators: 250 - 850 nm
Filters: 200 - 850 nm
With Stacker, minimum processing
time per plate
96 well: 20 seconds
384 well: 25 seconds
1536 well: 39 seconds
Barcode reader
Multi-directional, 1D and 2D camera-based
Software
Gen5™ Data Analysis Software
Absorbance
Light source
Xenon flash
Wavelength
selection
Monochromator
Wavelength range
230 - 999 nm, 1 nm increment
Bandwidth
2 nm (230 - 285 nm), 4 nm (>285 nm)
Dynamic range
0 - 4.0 OD
Resolution
0.0001 OD
Fluorescence Intensity
Time-Resolved Fluorescence
Alpha
Light source
100 mW 680 nm laser
Sensitivity
100 amol LCK peptide (384-well low volume plate)
Wavelength
selection
Filter cubes
Read speed
96 well: 30 seconds
384 well: 1 minute, 50 seconds
1536 well: 7 minutes, 20 seconds
Reagent Injectors
Number
2 syringe pumps
Dispense volume
5 - 1000 µL in 1 µL increments
Dead volume
1.1 mL, 100 µL with back flush
Light source
Xenon flash
Plate geometry
6- to 384-well plates, Petri dishes
Sensitivity
Monochromator:
Fluorescein 2 pM - top
Fluorescein 2.5 pM - bottom
Dispense precision
≤2% at 50 - 200 µL
Dispense accuracy
±1 µL or 2%
Filter cubes:
Fluorescein 0.2 pM - top
Fluorescein 1 pM - bottom
Wavelength
selection
Double grating monochromators (top/bottom)
Filter cubes (top/bottom)
Wavelength range
Monochromators: 250 - 850 nm
Filters cubes: 200 - 850 nm
Bandwidth
Monochromators: 16 nm excitation/emission
Filters: filter-dependent, from 5 nm to >100 nm
Detection system
Two matched PMTs top filter system
Low noise PMT bottom filter system
Red shifted PMT top/bottom monochromator system
Physical Characteristics
Power
250 Watts max.
Dimensions
15.4” W x 20.7” D x 16.1” H (39 x 52.5 x 41 cm)
Weight
Regulatory
78 lbs (35 kg)
CE and TUV marked. ROHS compliant. In Vitro Diagnostic
use models are available.
Specifications are subject to change. Performance values represent the average
observed factory test values.
www.biotek.com
13
Synergy™ H1 Hybrid Multi-Mode Microplate Reader
Synergy™ H1 Hybrid reader
is equipped with both monochromator and filter optical
systems. Synergy H1 provides
flexibility and performance, at a
very attractive price.
Flexibility at a Great Price
Synergy H1 is available in a
monochromator-only configuration.
Supporting top and bottom
fluorescence, UV-visible
absorbance and luminescence, it is
the most cost-effective solution of
its type on the market. Combined
with the Take3™ Micro-Volume
Plate for low volume 2 µL assays,
it is the perfect instrument for lifescience research laboratories.
Patented Hybrid Optical System
Adding the optional filter
module turns the Synergy H1
into an advanced Hybrid reader.
This patented optical design
is only available from BioTek.
Monochromators provide easeof-use and flexibility, while
filters provide increased optical
efficiency and sensitivity.
Gas Controller for Live
Cell Assays
orbital shaking, create the ideal
physiological environment needed
for assays using live cells.
Upgradable to Advanced
Read Modes
When equipped with the optional
filter module, Synergy H1 may be
used for fluorescence polarization
assays as well as Time-Resolved
Fluorescence (TRF) and
TR-FRET assays.
Dual Reagent Injector Module
An available Gas Controller for
Synergy H1 allows control
and monitoring of CO2 and O2
levels in the system. The Gas
Controller, along with advanced
temperature control to 45 °C and
For rapid, precise reagent
injection in all plate types, Synergy
H1 has an available dual reagent
injector module, ideal for inject/
read applications.
Typical Applications:
14
u
Nucleic acid quantification
u
Protein quantification
u
Enzyme kinetics
u
Biomarker quantification
u
ELISAs
u
Yeast kinetic analysis
u
Genetic analysis
u
Drug discovery
u
Cell proliferation
u
Cytotoxicity
u
Drug absorption and metabolism
u
Biologics drug discovery and development
u
Food safety
u
Environmental monitoring
Detection n Synergy H1
Synergy H1 shown with optional
Gas Controller module
Specifications
Fluorescence Intensity
General
Wavelength
selection
Hybrid Technology (patented)
Quadruple Monochromators and Filters/Dichroics
Detection mode
Monochromator system: Fluorescence, Luminescence,
UV-Visible Absorbance
™
Light source
Xenon flash
Sensitivity
Monochromators:
Top: Fluorescein 2.5 pM (0.25 fmol/well
384-well plate)
Bottom: Fluorescein 4 pM (0.4 fmol/well
384-well plate)
Filter system: Fluorescence, Time-Resolved Fluorescence,
Fluorescence Polarization, Luminescence
Read method
End point, kinetic, spectral scanning, well-area scanning
Microplate types
1- to 384-well plates
Other labware
Take3 Micro-Volume Plate
Temperature
control
4-Zone incubation to 45 °C; variation ±0.2 °C at 37 °C
with Condensation Control™
Shaking
Linear, orbital, double orbital
Software
Gen5™ Data Analysis Software
Automation
BioStack™ and 3rd party automation compatible
CO2 and O2
control
Range: 0 - 20% (CO2); 1 - 19% (O2)
Control Resolution: ±0.1% (CO2 and O2)
Stability: ±0.2% at 5% CO2; ±0.2% at 1% O2
Models for both CO2 and O2 or CO2 only are available
™
Absorbance
Light source
Xenon flash
Wavelength
selection
Monochromator
Wavelength range
230 - 999 nm, 1 nm increment
Bandwidth
4 nm (230 - 285 nm), 8 nm (>285nm)
Dynamic range
0 - 4.0 OD
Resolution
0.0001 OD
Pathlength
correction
Yes
Monochromator
wavelength
accuracy
±2 nm
Monochromator
wavelength
repeatability
±0.2 nm
OD accuracy
Filters/mirrors:
Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)
Wavelength
selection
Double grating monochromators (top/bottom) and,
Deep blocking bandpass filters/dichroic mirrors (top)
Wavelength range
Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option)
Dynamic range
5 decades
Detection system
Two PMT detectors: one for monochromator system, one
for filter system
Reading speed
96 wells: 11 seconds
384 wells: 22 seconds
Luminescence
Sensitivity
Monochromator system: 20 amol ATP (flash)
Filter system: 10 amol ATP (flash) 100 amol ATP (glow)
Wavelength range
300 - 700 nm
Dynamic range
>6 decades
Fluorescence Polarization
Sensitivity
1.2 mP standard deviation at 1 nM fluorescein
Wavelength range
280 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Light source
Xenon flash
Sensitivity
Europium 40 fM with filters (4 amol/well in 384-well plate)
Europium 1200 fM with monos (120 amol/well in 384-well
plate)
Wavelength range
200 - 700 nm (850 nm option)
Reagent Injectors
Number
2 syringe pumps
Dispense volume
5 - 1000 µL in 1 µL increments
<1% at 2.0 OD
<3% at 3.0 OD
Dead volume
1 mL, 100 µL with back flush
OD linearity
<1% from 0 to 3.0 OD
Plate geometry
6- to 384-well microplates, Petri dishes
OD repeatability
<0.5% at 2.0 OD
Dispense precision
Stray light
0.03% at 230 nm
Dispense accuracy
Reading speed
96 wells: 11 seconds
384 wells: 22 seconds
Specifications are subject to change. Performance values represent the average
observed factory test values.
<2% at 50 - 200 µL
±1 µL or 2%
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz. 130 Watts max.
Dimensions
15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm)
Weight
50 lbs (22.5 kg)
Regulatory
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic
use models are available.
www.biotek.com
15
Synergy™ 2 Multi-Mode Microplate Reader
The Synergy™ 2 has been
designed for life science research
and drug discovery applications.
It incorporates enhanced
fluorescence, luminescence and
absorbance optics for superior
performance. Advanced read
modes such as fluorescence
polarization and time-resolved
fluorescence are available as
individual, upgradable modules
and an available reagent
injection system expands the
applications range.
Best Price/Performance Ratio
The Synergy 2 incorporates
dedicated, optimized optical
paths for each detection mode
using filters for fluorescence
and a monochromator for
absorbance. The result is excellent
performance in all modes, at an
attractive price.
Sensitive Dichroic-based
Fluorescence Optics
The Synergy 2 fluorescence
optics are a step up from the
Synergy HTX, incorporating
dichroic mirrors, which decrease
background noise, as well as
a liquid-filled emission fiber
that increases the system’s light
collection efficiency. The result is
higher sensitivity for demanding
assays.
Dedicated Luminescence
Light Path
A dedicated liquid-filled light
guide coupled with a low
noise detector provides highperformance luminescence
detection, on par with dedicated
microplate luminometers.
Synergy 2 is DLReady™ certified
by Promega to run their DualLuciferase® assay system.
Typical Applications:
16
u
Nucleic acid quantification
u
Protein quantification
u
Enzyme kinetics
u
Biomarker quantification
u
ELISAs
u
Genetic analysis
u
Drug discovery
u
Cell proliferation
u
Cytotoxicity
u
Drug absorption and metabolism
u
Biologics drug discovery and development
u
Food safety
u
Biofuels research
u
Environmental monitoring
Detection n Synergy 2
Advanced, Modular Read Modes
In addition to the basic read
modes available on the
Synergy HTX, Synergy 2 offers
fluorescence polarization, timeresolved fluorescence and Alpha
detection modes, available as
individual, upgradable modules.
Specifications
Fluorescence Intensity
General
Detection mode
Fluorescence, Time-Resolved Fluorescence, Fluorescence
Polarization, AlphaScreen®/AlphaLISA®, Luminescence,
UV-Visible Absorbance
Read method
End point, kinetic, spectral scanning, well-area scanning
Microplate types
6- to 1536-well plates (luminescence 1- to 384-well plates)
PCR plates
Other labware
Take3™ Micro-Volume Plate
Temperature
control
4-Zone™ incubation to 65 °C; variation ±0.2 °C at 37 °C
Shaking
Linear
Software
Gen5™ Data Analysis Software
Automation
BioStack™ and 3rd party automation compatible
AlphaScreen/AlphaLISA
Light source
Tungsten halogen
Sensitivity
100 amol of biotinylated-LCK-P peptide, 25 µL/well in
384-well plate
Dynamic range
>6 decades
Reading speed
2 minutes (96-well plate)
Detection system
PMT
Absorbance
Light source
Xenon flash
Wavelength
selection
Monochromator
Wavelength range
200 - 999 nm, 1 nm increment
Bandwidth
Light source
Tungsten halogen
High energy DPR Xenon flash (optional)
Sensitivity
Top: Fluorescein 1 pM (0.2 fmol/well 96-well plate; 0.1
fmol/well 384-well plate)
Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate; 0.5
fmol/well 384-well plate)
Wavelength
selection
Deep blocking bandpass filters/dichroic mirrors
Wavelength range
300 - 700 nm with tungsten lamp (850 nm option)
200 - 700 nm with xenon lamp (850 nm option)
Dynamic range
>6 decades
Bandwidth
Filter dependent
Detection system
PMT
Reading speed
96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds
Luminescence
Sensitivity
10 amol ATP (flash) 100 amol ATP (glow)
Wavelength range
300 - 700 nm
Dynamic range
>6 decades
Detection system
PMT
Fluorescence Polarization
Light source
Tungsten halogen
High energy DPR Xenon flash (optional)
Sensitivity
3 mP at 1 nM fluorescein
2.4 nm
Wavelength
selection
Deep blocking filters/dichroic mirrors
Dynamic range
0 - 4.0 OD
Wavelength range
400 - 700 nm (320 - 850 nm option)
Resolution
0.0001 OD
Pathlength
correction
Yes
Monochromator
wavelength
accuracy
±2 nm
Monochromator
wavelength
repeatability
±0.2 nm
OD accuracy
<1% at 2.0 OD
<3% at 3.0 OD
OD linearity
<1% from 0 to 3.0 OD
OD repeatability
<0.5% at 2.0 OD
Stray light
0.03% at 230 nm
Reading speed
96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds
Time-Resolved Fluorescence
Light source
High energy DPR Xenon flash
Sensitivity
Europium 60 fM (12 amol/well 96-well plate;
6 amol/well 384-well plate)
Wavelength
selection
Deep blocking filters/mirrors
Wavelength range
200 - 700 nm (850 nm option)
Reagent Injectors
Number
2 syringe pumps
Dispense volume
5 - 1000 µL in 1 µL increment
Dead volume
1 mL, 100 µL with back flush
Plate geometry
6- to 96-well microplates
Dispense precision
<2% at 50 - 200 µL
Dispense accuracy
±1 µL or 2%
Physical Characteristics
Specifications are subject to change. Performance values represent the average
observed factory test values.
Power
250 Watts max.
Dimensions
17”W x 17.5”D x 14.5”H (43.5 x 44.5 x 37.3 cm)
Weight
Regulatory
60 lbs (27 kg)
CE and TUV marked. ROHS compliant. In Vitro Diagnostic
use models are available.
www.biotek.com
17
Synergy™ HTX Multi-Mode Microplate Reader
The Synergy™ HTX is an
entry-level, affordable and
upgradeable, multi-mode
microplate reader. Available
read modes include top and
bottom fluorescence, UV-visible
absorbance and luminescence
detection. Temperature control
to 50 ºC, shaking and advanced
Gen5™ data analysis software
are also included. A dual reagent
injector module is available for all
read modes and plate types.
Ideal for Basic Research
Applications
The Synergy HTX is the ideal
instrument for nucleic acid and
protein quantification, enzyme
assays, biomarker quantification
and ELISA assays, as well as cellbased assays (gene expression,
cellular growth, cytotoxicity).
Typical Applications:
XXAlphaScreen®/AlphaLISA®
18
u
Nucleic acid quantification
u
Protein quantification
u
Enzyme kinetics
u
Biomarker quantification
u
ELISAs
u
Genetic analysis
u
Cell proliferation
u
Cytotoxicity
u
Drug absorption and metabolism
u
Food safety
u
Environmental monitoring
Detection n Synergy HTX
AlphaScreen®/ AlphaLISA®
AlphaScreen and AlphaLISA
assays can be performed on
Synergy HTX with excellent
results. Alpha-capable
configurations add assay versatility
to basic research requirements.
Sensitive Filter-based
Fluorescence
Two excitation and two emission
filters are included with the reader,
and can be used for top and
bottom reading. Bottom reading
is usually recommended when
working with adherent cells, as it
often provides better signal-tobackground ratios. Top reading is
typically best for assays where the
fluorescence signal comes from
the solution.
Flexible Monochromator-based
Absorbance
All Synergy readers use
monochromators for absorbance
detection. This provides unlimited
wavelength selection from the low
UV to the near infrared, in
1 nm steps, and enables spectral
scanning.
Low-noise Luminescence
Detection
The Synergy HTX can automate
glow and flash luminescence assays,
thanks to its optional dual reagent
injector module. Typical assays
include ATP quantification as well as
luciferase gene expression assays.
Specifications
Fluorescence Intensity
General
Detection mode
Fluorescence, Time-Resolved Fluorescence (secondary
mode), Luminescence, UV-Visible Absorbance,
AlphaScreen/AlphaLISA
Light source
Tungsten halogen
Sensitivity
Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well
plate)
Wavelength
selection
Deep blocking filters
Wavelength range
300 - 700 nm (200 - 850 nm option)
Dynamic range
> 6 decades
Bandwidth
Filter dependent
Read method
End point, kinetic, spectral scanning, well-area scanning
Microplate types
6- to 384-well plates
PCR plates
Other labware
Take3 Micro-Volume Plate
Temperature
control
4-Zone™ incubation with Condensation Control™.
Ambient + 4 °C to 50 °C; variation ±0.2 °C at 37 °C
Shaking
Linear, orbital
Detection system
PMT
Software
Gen5™ Data Analysis Software
Reading speed
96 wells: 31 seconds
384 wells: 80 seconds
Automation
BioStack™ and 3rd party automation compatible
AlphaScreen/AlphaLISA
Light source
Tungsten halogen
Sensitivity
300 amol of biotinylated-LCK-P peptide, 25 µL/well in 384well plate
Dynamic range
>6 decades
Reading speed
2 minutes (96-well plate)
Detection system
PMT
Absorbance
Light source
Xenon flash
Wavelength
selection
Monochromator
Wavelength range
200 - 999 nm, 1 nm increments
Bandwidth
2.4 nm
Dynamic range
0 - 4.0 OD
Resolution
0.0001 OD
Pathlength
correction
Yes
Monochromator
wavelength
accuracy
±2 nm
Monochromator
wavelength
repeatability
±0.2 nm
OD accuracy
<1% at 2.0 OD
<3% at 3.0 OD
OD linearity
<1% from 0 to 3.0 OD
OD repeatability
<0.5% at 2.0 OD
Stray light
0.03% at 230 nm
Reading speed
96 wells: 14 seconds
384 wells: 26 seconds
Luminescence
Sensitivity
Multi-mode models: 30 amol ATP (flash)
Luminescence only: 10 amol ATP (flash)
Wavelength range
300 - 700 nm
Dynamic range
>6 decades
Detection system
PMT
Time-Resolved Fluorescence (secondary mode)
Light source
Xenon flash
Wavelength
selection
Monochromator
Reagent Injectors
Number
2 syringe pumps
Supported
detection modes
All modes
Dispense volume
5 - 1000 µL in 1 µL increments
Dead volume
1 mL, 100 µL with back flush
Plate geometry
6- to 384-well plates
Dispense precision
<2% at 50 - 200 µL
Physical Characteristics
Connectivity
1 USB, 1 RS232 for external PC control
Power
100 - 240 Volts AC. 50/60 Hz.
Dimensions
16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm)
Weight
40 lbs (18 kg)
Regulatory
Regulatory
CE and TUV marked. ROHS Compliant. In Vitro Diagnostic
use models are available.
Specifications are subject to change. Performance values represent the average
observed factory test values.
www.biotek.com
19
Multi-Mode Reader Comparison Chart
Which Multi-mode Reader
is right for you?
Synergy™ Neo
Cytation™ 5
Monochromator-based
UV-visible absorbance
•
•
Fluorescence top/bottom
•
•
Luminescence
•
•
Filtered luminescence
•
•
Injectors
•
•
TRF & TR-FRET
•
•
•
•
Laser AlphaLISA/AlphaScreen
•
•
Hybrid Technology
•
•
Automation ready/BioStack compatible
•
•
Dual PMT read head
•
Key Features
Fluorescence polarization
Standard AlphaLISA /AlphaScreen
®
®
™
™
Performance Specifications
Fluorescein typical – top
2 pM (monos) / 0.2 pM (filters)
2.5 pM (monos) / 0.25 pM (filters)
1 pM (filters)
4 pM (monos)
5 amol
10 amol
Polarization typical – 1 nM Fluorescein
1 mP
1.2 mP standard deviation
Europium typical
40 fM
40 fM (filters)
100 amol
100 amol
6/11
11/22
1 to 1536
1 to 384
Fluorescein typical – bottom
ATP typical – flash luminescence
AlphaScreen typical - LCK peptide
Fastest read speed
96-/384-well plates (seconds)
General Specifications
Microplate types
Gas Controller compatible
Barcode reader option
•
Take3™ Micro-Volume Plate compatible
•
•
to 65 ºC
to 65 ºC
•
•
6 filter sets
2 filter sets
Filter dependent
Mono 16 nm
Filter dependent
Mono from 9 nm to 50 nm
Temperature control system
Condensation Control™
Filter capacity
Fluorescence bandwidth
20
•
Detection n Multi-Mode Reader Comparison Chart
Synergy H1
Synergy 2
Synergy HTX
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
(secondary mode)
•
•
•
•
•
•
•
2.5 pM (monos) / 0.25 pM (filters)
1 pM
5 pM
4 pM (monos)
5 pM
5 pM
10 amol
10 amol
30 amol/10 amol (luminescence only
configuration)
1.2 mP standard deviation
3 mP
n/a
40 fM (filters)
60 fM
n/a
n/a
100 amol
300 amol
11/22
11/22
14/26
1 to 384
1 to 1536
6 to 384
•
•
•
to 45 ºC
to 65 ºC
to 50 ºC
•
•
•
•
2 filter sets
4 filter sets
4 filter sets
Filter dependent
Mono 16 nm
Filter dependent
Filter dependent
www.biotek.com
21
FLx800™ Fluorescence Microplate Reader
The compact FLx800™ fluorescence
reader provides high performance
in 6- to 384-well microplates at an
attractive price. Options include top
and bottom detection, temperature
control and reagent injector.
Great Price/Performance Ratio
The FLx800 uses top and bottom
bifurcated quartz fibers to ensure
strong sample excitation and efficient
collection of the emitted signal.
This reader combines sensitivity,
convenience and ease of use all at a
great price.
Bottom Reading and FRET Detection
The bottom detection system uses
a large 5 mm diameter quartz fiber
optimized for cell-based assays in
96-well plates and smaller densities.
The reader may be equipped with up
to 4 filter sets and may be used to run
cell-based FRET assays.
Sensitive Luminescence Detection
The FLx800’s detector provides
very high sensitivity when running
luminescent assays. ATP or luciferase
can be quantified down to very low
concentrations using the reader’s
photon integration mode.
Fluorescent Ion Channel Assays
A syringe pump injector is available
as an option to automate fluorescent
ion-channel assays. This system is used
to inject a trigger reagent that induces
a fast change in fluorescent signal. The
FLx800 kinetically monitors the signal
just after injection.
Typical Applications:
u
u
u
u
u
Nucleic acid quantification
Protein quantification
Enzyme kinetics
Genetic analysis by fluorescence
Cellular analysis by fluorescence
Specifications
General
Detection mode
Fluorescence, Luminescence
Read method
End point, kinetic and area scanning under computer
control
Microplate types
6- to 384-well plates
Temperature control
4 °C above ambient to 50 °C (I models)
Shaking
Linear (I models)
Software
Gen5™ Data Analysis Software
Onboard software
55 user-programmable protocols
Fluorescence Intensity
Light source
Tungsten halogen
Sensitivity
Fluorescein 5 pM (1 fmol/well 96-well plate)
Wavelength selection
Deep blocking filters
Wavelength range
300 - 700 nm (850 nm option)
Dynamic range
5 decades
Luminescence
Sensitivity
100 amol ATP (flash)
Wavelength range
300 - 700 nm
Dynamic range
5 decades
Reagent Dispensers
Number
1 syringe pump
Dispense volume
5 - 1000 µL in 1 µL increments
Minimum prime
volume
1.1 mL, 100 µL with backflush
Physical Characteristics
Power
100 - 240 Volts AC.
Dimensions
15"W x 16"D x 9"H (38.1 x 40.64 x 22.89 cm)
Weight
30 lbs (13.6 kg)
50/60 Hz.
Regulatory
Regulatory
CE and TUV marked. ROHS compliant.
In Vitro Diagnostic use models are available.
Specifications are subject to change. Performance values represent the average
observed factory test values.
22
Detection n FLx800
PowerWave™ HT Microplate Spectrophotometer
The PowerWave™ HT is a high
throughput, robot friendly microplate
spectrophotometer with a very small
footprint, ideal for integration into
automated systems. Wavelength
selection in 1 nm increments,
temperature control and superior
performance up to 4.0 OD add to its
appeal for a variety of assay needs.
High Speed, Higher Throughput
In automation and high throughput,
timing is everything… With 8 reading
channels, the PowerWave HT can read a
96-well plate in 5 seconds.
Low Stray Light Monochromator
Optics
PowerWave HT’s monochromator optics
pre-select the measurement wavelength
before light goes through the sample.
This results in very low stray light
reaching the detector…with the added
benefit of excellent performance even
at high optical densities.
Specifications
General
Detection mode
Absorbance
Read method
End point, kinetic, spectral scanning, linear scanning
Microplate types
96- and 384-well plates
Temperature control
4 Zone™ incubation: Ambient + 4 ºC to 50 °C; variation
±0.2 °C at 37 °C
Shaking
Linear
Software
Gen5 Data Analysis Software
Automation
BioStack and 3rd party automation compatable
Absorbance
Light source
Xenon flash lamp
Wavelength selection
Monochromator, selectable 1 nm or greater
increments
Wavelength range
200 - 999 nm
BioStack™ Compatible for Benchtop
Automation
Bandwidth
5 nm
Dynamic range
0 - 4.0 OD
When walkaway benchtop automation
is required, the PowerWave HT, coupled
with BioStack, provides a compact
system for rapid processing of up to 50
plates at a time.
Resolution
0.001 OD
Monochromator
wavelength accuracy
±2 nm
Monochromator
wavelength
repeatability
±0.2 nm
OD accuracy
1% ±0.01 OD
OD linearity
±1% typical
OD repeatability
0.5% ±0.005 OD
Stray light
0.03% at 230 nm
Reading speed
96 wells: 5 seconds
384 wells: 11 seconds
Gen5™ Control = Assay Flexibility
Gen5 Data Analysis Software not
only allows easy control of all the
functionality of the PowerWave HT,
it also supports a vast number of
applications in absorbance. Quick
export to Microsoft® Excel® or use
Gen5’s powerful data analysis tools
to make quick work of the most
complex assays.
Typical Applications:
u Enzyme kinetics
u ELISAs
u Genetic analysis by colorimetry
u Cellular analysis by colorimetry
u Cell proliferation
Detection n PowerWave HT
Physical Characteristics
Power
100 - 240 Volts AC.
Dimensions
8.5”W x 16"D x 8.5"H (21.6 x 40.6 x 21.6 cm)
Weight
24 lbs (11 kg)
50/60 Hz.
Regulatory
Regulatory
CE and TUV marked. ROHS compliant.
In Vitro Diagnostic use models are available.
Specifications are subject to change. Performance values represent the average
observed factory test values.
www.biotek.com
23
Epoch™ 2 Microplate Spectrophotometer
Epoch™ 2 is a compact
monochromator-based microplate
spectrophotometer for 6- to
384-well microplates, cuvettes
and 2 µL measurements. Epoch 2
features a 10” color touchscreen
interface with easy to navigate
controls, and full onboard
Gen 5™ software for data
collection, powerful analysis
and flexible export and report
options. Incubation, shaking
and robot compatibility are
standard features.
UV-Vis Measurements
Epoch 2’s monochromator-based
optics offers wavelength selection
from 200 nm to 999 nm – for
applications from nucleic acid
quantification to ELISA, without
using filters. Epoch 2 can measure
up to 48 2 µL samples in the
unique Take3 Micro-Volume plates
for rapid direct quantification. An
optional cuvette port provides
quick 1 cm measurements,
making Epoch 2 a versatile
spectrophotometer for multiple
applications.
Touch. Run. Done.
Designed for easy-to-use, yet
powerful functionality, Epoch 2
features a 10” color touchscreen
interface, WiFi, Bluetooth and
USB connectivity and flash drive
storage. It’s a self-contained
computer, in a space and cost
saving design, configurable for the
laboratory’s needs today and in
the future.
Full Gen5 Data Analysis Software
With Epoch 2, “onboard software”
doesn’t mean “limited software”.
Complete reader control, protocol
design, data analysis and export/
report functions are at your
fingertips. For applications in
microplates, cuvettes or
Typical Applications:
XXELISA
XXEnzyme
kinetics
XXNucleic
acid and protein quantification
XXCell
proliferation
XXCytotoxicity
XXSpectral
scanning
XXReactive
oxygen species
XXFood
safety and quality
XXBacterial
XXTotal
protein determination
XXNucleic
24
Detection n Epoch 2
identification
acid purity assessment
Take3 plates, Gen5 offers the
same intuitive navigation and full
capability as an external computer.
With Gen5 on the Epoch 2 –
there’s no need for a dedicated
computer – it’s all built-in!
Advanced 4-Zone™ Incubation
Epoch 2 features BioTek’s 4-Zone
natural convection incubator up
to 65 °C with minimal variation
across the plate – ideal for a
wide range of temperaturesensitive assays. Epoch 2’s
unique Condensation Control™,
solves the common problem of
condensation build up on plate
lids during incubated kinetic runs.
The Condensation Control feature
allows a temperature gradient
between top and bottom heating
surfaces, minimizing the formation
of condensation on plate lids and
ensuring consistent results.
Specifications
General
Wavelength selection
Monochromator
Read method
Endpoint, kinetic, spectral scanning, well area scanning
Microplate types
6-, 12-, 24-, 48-, 96-, and 384-well plates
Other labware
Take3™ Micro-Volume Plates (optional)
BioCell™ (optional)
Standard cuvette (with “C” configurations)
Temperature control
4-Zone incubation: Ambient +4°C to 65 °C with Condensation Control
Variation ±0.2 0C at 37 0C
Shaking
Linear, orbital, double orbital
Software
Onboard touchscreen: Gen5™ TS Data Analysis Software
Non-touchscreen: Gen5™ Data Analysis Software
Automation
BioStack™ and 3rd party automation compatible
Wavelength range
200 –999 nm, selectable in 1 nm increments
Light source
Xenon flash
Dynamic range
0.0 - 4.0 OD
Resolution
0.0001 OD
Bandwidth
2.9 nm
Performance (Performance noted below is for 96-well microplates and cuvette measurements)
Monochromator wavelength accuracy
± 2 nm
Monochromator wavelength repeatability
± 0.2 nm
OD accuracy
0 to 2.0 OD ± 1% ± 0.010
2.0 to 2.5 OD ± 3% ± 0.010
OD linearity
0 to 2.0 OD ± 1% ± 0.010
2.0 to 2.5 OD ± 3% ± 0.010
OD repeatability
0 to 2.0 OD ± 1% ± 0.005
2.0 to 2.5 OD ± 3% ± 0.005
Stray light
0.03% at 230 nm
Read speed
96 wells sweep read: 8 seconds
384 wells sweep read: 14 seconds
Physical Characteristics
Connectivity
1 USB for external PC control
1 USB for peripheral devices
Power
100 - 240 Volts AC.
Dimensions
With touchscreen: 15.5” D x 12.5” W x 13” H (39.3 x 32 x 33 cm)
Without touchscreen: 15.5” D x 12.5” W x 8” H (39.3 x 32 x 20.3 cm)
Weight
With touchscreen 25 lbs (11.3 kg)
Without touchscreen 20 lbs (9.1 kg)
50/60 Hz.
Regulatory
Regulatory
CE and TUV marked, RoHS compliant. Models for In Vitro Diagnostic use are available.
Specifications subject to change. Performance values represent the average observed factory test values.
www.biotek.com
25
Epoch™ Microplate Spectrophotometer
Epoch™ is a monochromatorbased microplate spectrophotometer that offers superior
functionality for the life science
laboratory at an accessible price.
Controlled by the powerful, yet
easy-to-use Gen5™ Data Analysis
Software, Epoch is designed to
be the new lab workhorse for
a wide variety of applications.
For walk-away automation, an
optional BioStack™ compatible
Epoch is available.
200 nm to 999 nm
Wavelength Range
The monochromator-based
optical system in Epoch allows any
wavelength selection between 200
and 999 nm in 1 nm increments.
No filters required! From low UV
nucleic acid measurements to
standard ELISA assays, Epoch is
ideally suited to the life science
laboratory where application
flexibility is required.
6- to 384-well Microplate
Reading
Epoch’s optical and mechanical
systems are designed to provide
optimal measurements in a variety
of microplates. The area scanning
capability provides multiple
measurements across larger
diameter wells, resulting in more
meaningful data analysis.
Typical Applications:
26
u
Nucleic acid quantification
u
Protein quantification
u
260/280 and 260/230 purity measurements
u
ELISA
u
Enzyme kinetics
u
Cytotoxicity
u
Cell proliferation
u
Micro-volume assays with Take3 plate
Detection n Epoch
Take3™ Micro-Volume Plate
Compatible
When sample size matters, as in
critical nucleic acid and protein
quantification, the Take3 plate
provides up to sixteen 2 µL
measurements – without needing
to dilute important samples.
End Point, Kinetic, Spectral
Scanning
There’s no need to buy
expensive instrumentation to
perform a variety of absorbance
measurements. Epoch, driven by
Gen5 Data Analysis Software, is
the ultimate high-value system
with maximum assay flexibility.
Specifications
General
Wavelength selection
Monochromator
Read method
End point, kinetic, spectral scan, well area scan
Microplate types
6- to 384-well plates
Other labware
Take3™ Micro-Volume Plate, BioCell
Software
Gen5™ Data Analysis Software
Automation
BioStack™ and 3rd party automation compatible (‘R’ configuration)
Absorbance
Wavelength range
200 nm - 999 nm, selectable in 1 nm increments
Light source
Xenon flash
Bandwidth
5 nm
Dynamic range
0 - 4.0 OD
Resolution
0.0001
Monochromator wavelength accuracy
±2 nm
OD accuracy
0 to 2 OD: ±1% ±0.010 OD
2 to 2.5 OD: ±3% ±0.010 OD
OD linearity
0 to 2 OD: ±1% ±0.010 OD
2 to 2.5 OD: ±3% ±0.010 OD
OD repeatability
0 to 2 OD: ±1% ±0.005 OD
2 to 2.5 OD: ±3% ±0.005 OD
Reading speed
96 wells: 15 seconds
384 wells: 31 seconds
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz.
Dimensions
12” W x 12.5” D x 7.7” H (30.5 cm x 31.8 cm x 19.6 cm)
Weight
<15 lbs (6.8 kg)
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
Specifications subject to change. Performance values represent the average observed factory test values.
www.biotek.com
27
ELx808™ Absorbance Microplate Reader
Excellent optical performance
and superior incubation are
among the top features of
this multi-channel reader. The
ELx808™ is suitable for a wide
array of applications, from
endpoint ELISAs to kinetic cell
growth studies.
4-Zone™ Temperature Control
For temperature sensitive assays,
there is no better incubation
system in this microplate reader
class than the ELx808. The natural
convection heating is software
controlled for consistency and
performance over time.
Fast Measurement
The ELx808 can collect kinetic
data in intervals as short as 6
seconds, for the most demanding
assays. Gen5™ Data Analysis
Software provides multiple
kinetic and end point data
analysis options for a variety of
applications.
Superior Optical Performance
The ELx808 can accommodate up
to six absorbance filters, and its
optical channels are staggered to
prevent crosstalk between wells.
The reference channel eliminates
channel-to-channel variation. This
unique design gives the ELx808 its
proven optical performance.
Typical Applications:
General
Detection mode
Absorbance
Read method
End point, kinetic, linear scanning
Microplate types
96-well plates
Temperature control
4-Zone incubation to 50 °C (ELx808IU)
Shaking
Linear
Software
Gen5 Reader Control Software
(Gen5 Data Analysis Software optional)
Onboard software
55 user-programmable protocols
Absorbance
Light source
Tungsten halogen
Wavelength selection
Filters
Wavelength range
380 - 900 nm
340 - 900 nm (ELx808IU)
Bandwidth
10 nm
Dynamic range
0 - 4.0 OD
Resolution
0.001 OD
Filter wheel capacity
6 positions
Filters supplied
4 filters (5 with UV option)
OD accuracy
<1% at 2.5 OD
<2% at 3.5 OD
OD linearity
<1% at 2.5 OD
OD repeatability
<0.5% at 2.5 OD
<1.5% at 3.5 OD
Reading speed
96 wells: 8 seconds
Physical Characteristics
ELISA
Power
100 - 240 Volts AC.
u
Enzyme kinetics
Dimensions
15.5"W x 16"D x 8.75"H (39.4 x 40.6 x 22.2 cm)
u
Endotoxin assays
Weight
30 lbs (13.6 kg)
u
Cell growth studies
u
Cytotoxicity
u
Protein assays
u
28
Specifications
Detection n ELx808
50/60 Hz.
Regulatory
Regulatory
For In Vitro Diagnostic use. CE and TUV marked.
ROHS compliant.
Specifications subject to change. Performance values represent the average
observed factory test values.
ELx800™ Absorbance Microplate Reader
The ELx800™ is a compact,
robust microplate reader ideally
suited for applications within the
clinical and life science research
laboratories.
Reliable And Robust Design
These characteristics are the
reason there have been more
than 25,000 ELx800s placed in
laboratories around the globe.
There simply isn’t a more reliable
reader with proven performance
than the ELx800.
Gen5™ Software Expands
Versatility
Under computer control by Gen5
Data Analysis Software, ELx800
applications are expanded to
include kinetic and well area
scanning measurements. Data
analysis and reporting/exporting
features in Gen5 are completely
customizable to suit your
laboratory’s requirements.
Specifications
General
Detection mode
Absorbance
Read method
End point, kinetic and area scanning under computer
control
Microplate types
6- to 384-well plates
60/72/96-well Terasaki plates (NB option)
Software
Gen5 Reader Control Software
(Gen5 Data Analysis Software optional)
Onboard software
55 user-programmable protocols
Absorbance
Light source
Tungsten halogen
Wavelength selection
Filters
Wavelength range
400 - 750 nm
340 - 750 nm (UV option)
Bandwidth
10 nm
Dynamic range
0 - 3.0 OD
Resolution
0.001 OD
Filter wheel capacity
5 positions
Filters supplied
4 filters (5 with UV option)
OD accuracy
<1% at 2.0 OD
OD linearity
<1% at 2.0 OD
<3% at 3.0 OD
OD repeatability
<0.5% at 2.0 OD
Reading speed
96 wells: 30 seconds
High Performance, High Value
The ELx800 comes with four filters
(user-selectable wavelengths), with
a five filter capacity. In addition
to standard 96-well microplate
reading, the ELx800 offers 6-, 12-,
24-, 48- and optional 384-well
microplate reading, to fit a variety
of assay needs.
Physical Characteristics
Typical Applications:
u
ELISA
u
Protein assays
u
Cytotoxicity
Power
100 - 240 Volts AC.
Dimensions
15”W x 16.5”D x 7”H (38.1 x 41.9 x 17.8 cm)
Weight
18.5 lbs (8 kg)
50/60 Hz.
Regulatory
Regulatory
For In Vitro Diagnostic use. CE and TUV marked.
ROHS compliant.
Specifications subject to change. Performance values represent the average
observed factory test values.
Detection n ELx800
www.biotek.com
29
Absorbance Reader Comparison Chart
Which Absorbance Reader is right for you?
PowerWave™ HT
Epoch™ 2
Epoch™
Monochromator
based
Monochromator
based
Monochromator
based
200 - 999
200 - 999
200 - 999
96 and 384
6 to 384
6 to 384
0 - 4.0
0 - 4.0
0 - 4.0
to 50 °C
to 65 °C
Linear
Linear, orbital, double-orbital
Cuvette adapter
Cuvette port (optional),
Take3 or cuvette adapter
Take3 or cuvette adapter
n/a
n/a
n/a
•
•
("R" configuration)
Gen5
Gen5
Gen5
•
•
5
8
15
OD accuracy
1% +0.01 OD
0 to 2.0 OD: ±1% ±0.010 OD
2.0 to 2.5 OD: ±3% ±0.010 OD
0 to 2.0 OD: +1% +0.010 OD
2.0 to 2.5 OD: +3% +0.010 OD
OD linearity
±1%
0 to 2.0 OD: ±1% ±0.010 OD
2.0 to 2.5 OD: ±3% ±0.010 OD
0 to 2.0 OD: +1% +0.010 OD
2.0 to 2.5 OD: +3% +0.010 OD
0.5% ±0.005 OD
0 to 2.0 OD: ±1% ±0.005 OD
2.0 to 2.5 OD: ±3% ±0.005 OD
0 to 2.0 OD: +1% +0.005 OD
2.0 to 2.5 OD: +3% +0.005 OD
0.001 OD
0.0001 OD
0.0001 OD
Key Features
Wavelength selection
Wavelength range (nm)
Microplate types
Absorbance range
Temperature control
Shaking
Cuvette measurement
Filter capacity
Automation ready/BioStack™
compatible
Gen5™ Software version included
Take3 Micro-Volume Plate
compatible
™
Fastest read speed: 96 wells (seconds)
Typical Performance
OD repeatability
Resolution
30
Detection n Absorbance Reader Comparison Chart
ELx808 IU
ELx808™
ELx800™
ELx800 UV
ELx800 NB
Filter-based
Filter-based
Filter-based
Filter-based
Filter-based
340 - 900
380 - 900
400 - 750
340 - 750
400 - 750
96
96
6 to 96
6 to 96
6 to 384
0 - 4.0
0 - 4.0
0 - 3.0
0 - 3.0
0 - 3.0
to 50 °C
Linear
Linear
6
6
5
5
5
Gen5RC
Gen5RC
Gen5RC
Gen5RC
Gen5RC
8
8
30
30
30
<1% at 2.5 OD
<2% at 3.5 OD
<1% at 2.5 OD
<2% at 3.5 OD
<1% at 2.0 OD
<1% at 2.0 OD
<1% at 2.0 OD
<1% at 2.5 OD
<1% at 2.5 OD
<1% at 2.0 OD
<3% at 3.0 OD
<1% at 2.0 OD
<3% at 3.0 OD
<1% at 2.0 OD
<3% at 3.0 OD
<0.5% at 2.5 OD
<1.5% at 3.5 OD
<0.5% at 2.5 OD
<1.5% at 3.5 OD
<0.5% at 2.0 OD
<0.5% at 2.0 OD
<0.5% at 2.0 OD
0.001 OD
0.001 OD
0.001 OD
0.001 OD
0.001 OD
www.biotek.com
31
Gen5™ Data Analysis Software
Gen5™ Data Analysis Software
incorporates over 30 years of
experience and user feedback
into outstanding microplate
reader software. Gen5 is a
unique combination of power
and ease-of-use that drives
productivity and saves time. Use
Gen5 to control BioTek’s readers
and export data, or as a fully
integrated processing tool.
Beginner-friendly Software
Gen5 is built around logical
laboratory workflows to read
microplates and produce/analyze
data. In Gen5, you simply click
“Read Now” and follow the
prompts. At the end of the read,
answer the question: “Do you
want to export to Excel?” With
Gen5 you don’t have to spend
hours figuring out how to get
things done.
Powerful Functionality
Gen5 comes with powerful built-in
tools such as 4-P and 5-P curve
fits with or without weighting,
parallel-line analysis, advanced
kinetic analysis, and much more.
The software has been specifically
designed to analyze matrices of
data that are difficult to process in
Microsoft® Excel® spreadsheets.
Special attention has been placed
on result presentation so complex
data can be displayed in a clean,
colorful way to facilitate data
interpretation.
users can upload files to share
their experiments and sample data
with other Gen5 users.
Gen5 Secure: You Are In Control
Gen5 Secure is for you if secured
data storage, user group
management or 21 CFR Part 11
compliance are a core part of
your requirements. It includes
extra features such as 25 licenses
per copy, quality control trending
module with Levey-Jennings
charts and automatic email
notification on trigger events.
All-in-One Solution
Up-to-date Web-based
Sample Files
A searchable library of sample
files with data is available on the
BioTek web site. Existing files are
kept up to date and new files are
added on a regular basis. Gen5
Control any BioTek reader,
automate the entire process and
produce publication-ready reports
with one integrated, powerful
software. It doesn’t get more
efficient than this.
High-resolution 99 x 99 area scan
Parallel line analysis and EC50 determinations
Multiple window views of plate data and results
32
Detection n Gen5 Software
Gen5™ Comparison Chart
Gen5 RC
Gen5
Gen5 Image+
Gen5 Secure
Gen5 Secure Image+
•
•
•
•
•
Absorbance, fluorescence, luminescence, time-resolved
fluorescence, fluorescence polarization, Alpha
•
•
•
•
•
End point, kinetic, spectral scanning, linear scanning,
Take3 interface
•
•
•
•
•
•
•
•
•
Readers supported
All Cytation and Synergy readers; Epoch 2 (external),
Epoch, PowerWave HT, FLx800, ELx808, ELx800
Read Modes and Methods
Single and Multi-Mode Data Analysis
Qualitative, quantitative, kinetic, spectral analysis, custom
transformations, EC50, parallel line analysis, Z’ calculation,
validation and cutoff formulae
Imaging Modes and Methods
Fluorescence, brightfield, phase contrast, H&E
•
•
•
•
•
Single color, multi-color, montage, Z-stacking
•
•
•
•
•
•
•
•
•
Image Processing and Analysis
Cell counting
Subpopulation analysis
•
•
Hit-picking
•
•
Z-projection
•
•
Digital phase contrast
•
•
Image stitching
•
•
Security / 21CFR
User groups, single sign-on (SSO) option
•
•
Secure database data storage, audit trails
•
•
Electronic signature, email notification
•
•
available
available
Validation Tools
Gen5 Validation Package
available
available
available
www.biotek.com
33
Take3™ Micro-Volume Plate
Quickly quantify ultra-low volume
samples of DNA, RNA and protein.
Measure up to 48 samples with
volumes as low as 2 µL without
dilution. Take3™ can be used to
measure a standard cuvette or
patented BioCells™ for quick 1 cm
measurements. Low volume, higher
throughput is available with the
Take3 Trio.
Compatible with Most BioTek
Detection Systems
Epoch™, Synergy™ and Cytation™
reader functionality can easily reach
into the micro-volume range using the
Take3 plate. Measure multiple 2 µL
samples, cuvettes or BioCells. Adding
the Take3 plate to a BioTek detection
system creates an incredibly versatile
workstation for a variety of applications.
Unique Robust Construction
and Easy Maintenance
The anodized aluminum base
construction, precision crafted slides and
hydrophobic sample surfaces make
Typical Applications:
u
u
34
Micro-volume DNA, RNA and protein
quantification
Micro-volume fluorescence
measurements in Synergy and
Cytation readers
u
Fluorescent dye incorporation
measurements
u
Spectral scanning in micro-volume,
cuvette or BioCell
Detection n Take3 Plate
pipetting simple and cleanup effortless.
For routine cleaning of the sample
surfaces, a laboratory wipe is all that’s
needed. If a slide becomes damaged,
replacement is easy – no need to return
the Take3 to the factory for repair
or calibration.
Gen5 Take3 Module: Automated DNA,
RNA and Protein Quantification
It couldn’t be easier to get multiple
(up to 48) nucleic acid or protein
sample results. Gen5’s Take3 module
includes pre-programmed protocols
with immediate results output including
spectral scans and purity ratios. There’s
no need for complicated configuration
or calculation.
Specifications
Take3
Take3 Trio
2 µL sample capacity
16
48
Detection limit
2 ng/µL dsDNA
2 ng/µL dsDNA
BioCell capacity
2
2
Cuvette capacity
1
n/a
Specifications are subject to change.
Reader Accessories
BioTek offers a wide range of accessories to help increase productivity, expand your plate reader’s capabilities, and
maintain the performance of your BioTek microplate reader system. See our web site for a complete listing of available
accessories.
Instrument Qualification
Dual Reagent Injector Module
Automate inject/read assays such
as flash luminescence assays (ATP,
luciferase) and fluorescent ion
channel assays on all Synergy and
Cytation readers.
See the Compliance Section on
pages 62-63 for details about
BioTek’s product qualification
tools and services.
Filters and Mirrors
Gas Controller
The Gas Controller module for
the Synergy H1 and Cytation
allows full control over CO2 and
O2 concentrations to modulate the
environment for microplate-based
live cell assays.
A full range of standard and custom
filters and dichroic mirrors are
available for applications from the
low UV to the near infrared.
Gen5 Secure Software
BioStack™ Microplate Stacker
Upgrade to Gen5 Secure for
21 CFR Part 11 compliance,
user management features, data
encryption and much more.
Automate routine processes with
this compact stacker. BioStack is
also compatible with BioTek’s liquid
handling instruments.
www.biotek.com
A searchable Accessory database, including descriptions and photos, is available on www.biotek.com
35
36
Liquid Handling n Overview
Liquid
Handling
BioTek offers a range of compact and affordable
solutions for your laboratory’s specific liquid handling
needs. BioTek is world renowned for manufacturing
the most reliable and versatile microplate washers
on the market. From basic ELISA to sensitive cell
washing to bead washing (including Luminex®
xMAP® technology), the EL406™, 405™ Touch,
ELx50™ and the new MultiFlo™ FX are configured with
many options to meet a myriad of assay requirements.
BioTek’s microplate reagent dispensers incorporate
two optimized fluid delivery technologies to
address different liquid handling requirements.
From milliliters down to one microliter, our reagent
dispensers offer simple, repeatable and precise
liquid delivery throughout their defined volume
range. Single, 8- and 12-channel transfer tools are
available, along with bulk reagent dispensers, to
meet varied liquid handling requirements.
www.biotek.com
37
EL406™ Microplate Washer Dispenser
The EL406™ Combination
Washer Dispenser is the only
instrument on the market
offering fast microplate washing
together with BioTek’s unique
Parallel Dispense™ technologies
for optimized liquid handling
processes.
Unattended Automation of
ELISAs and Cell-based Assays
The EL406 integrates 96-, 384and 1536-well microplate washing
with three dispensers in one
compact instrument. Now you can
simply press a button and walk
away, or automate an entire batch
by adding a BioStack™ Microplate
Stacker. A range of EL406 models
provides configurability for a lab’s
specific requirements.
Patented Dual-Action™ Manifold
and Ultrasonic Advantage™
The EL406 incorporates BioTek’s
Dual-Action manifold for thorough
yet gentle washing of loosely
adherent cell layers, and Ultrasonic
Advantage for automated wash
manifold maintenance.
Parallel Dispense Technologies
The EL406 eliminates the need to
choose a dispensing technology
by offering both peristaltic
and syringe pumps on a single
platform.
Typical Applications:
38
u
ELISA automation
u
MSD assay automation
u
High content screening immunocytochemistry
u
Cell-based assays
u
FLIPR® Ca2+ flux
u
Magnetic bead assay automation
u
Polystyrene bead assay automation
u
Drug transport assays
u
Automated cell washing, fixing and staining for cellular imaging
u
SiLA compliant integration (with LHC software)
Liquid Handling n EL406
Fast and Efficient Biomagnetic
Separation and Vacuum
Filtration
The EL406 automates full
microplate washing of magnetic
microspheres used in an increasing
number of multiplex assays.
Developed in conjunction with
Luminex® xMAP® technology
leaders, BioTek’s separation
modules incorporate high
energy neodymium iron boron
magnets for speed and efficiency.
An available vacuum filtration
module makes the EL406 also well
suited for polystyrene beads and
filtration-to-waste processes.
Specifications
General
Assays
ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead (optional)
• Multiplex assays
• Bead-based ELISA
Filtration-to-waste processes (optional)
Separation
Biomagnetic separation, vacuum filtration (optional)
Microplate types
96-, 384- and 1536-well plates (model dependent)
Low profile and standard height
Solid and filter bottom (optional)
Shaking
Programmable in minutes and seconds, up to 60 minutes
Intensities - slow, medium, fast or variable
Soak time
Programmable in minutes and seconds, up to 60 minutes
Magnet
High strength 96- and 384-well designs
Flat
• Flat-bottom well - beads pulled to band across well bottom
• Round-bottom well - beads pulled to button at well bottom
Ring - beads pulled to 4-zone ring at well bottom
Safety/
convenience/
maintenance
Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Pre-programmed maintenance routines
Automation
Compatible with BioStack (excluding filter bottom plates) and
3rd party automation
Onboard software
Create, edit or run multiple protocols
Software
Liquid Handling Control™, for PC protocol programming and
execution (optional). SiLA compliant driver available.
Washing
Manifold types
96-well washing:
96-tube (8x12) manifold - 316 stainless steel tubes
Ultrasonic
cleaning
Built-in ultrasonic bath to remove protein and salt crystal buildup
Supply bottle
4 L or 10 L (optional)
Dispensing - Peristaltic Pump
Manifold types
8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316
stainless steel or polypropylene plastic tips
Dispensing speed
10 µL/well, 96 wells, 8-tip cassette: 8 seconds
5 µL/well, 384 wells, 8-tip cassette: 12 seconds
1 µL/well, 1536 wells, 8-tip cassette: 27 seconds
Volume range
500 mL - 3,000 µL/well
Selectable in 1 µL increments
Fluid delivery
One positive displacement peristaltic pump
Flow rates
Low, medium or high
Cassette size
1 µL
5 µL
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: ±2.0% at 5 µL
Dispense Precision: ≤2.5% CV at 5 µL
Minimum Prime Volume (30” tubing length): 4.23 mL
10 µL
Recommended Volume Range: 10 - 3000 µL
Dispense Accuracy: ±2.0% at 10 µL
Dispense Precision: ≤2.0% CV at 10 µL
Minimum Prime Volume (30” tubing length): 7.36 mL
Sterilization
Manifold types
Fluid delivery
Solid bottom plates, 1 asp./disp. cycle:
300 µL/well, 96 wells, 96-tube manifold: 13 seconds
100 µL/well, 384 wells, 192-tube manifold: 17 seconds
10 µL/well, 1536 wells, two 32-tube manifolds: 36 seconds
96-/384-well washing:
One positive displacement pump
1536-well washing:
Two positive displacement syringe drives
96-well dispensing:
One 16-tube (2 x 8) manifold - 316 stainless steel tubes
96-/384-well dispensing:
Two 16-tube (1 x 16) manifolds - 316 stainless steel tubes
1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 stainless
steel or 316 stainless steel tubes
384-well washing:
Dual-Action 192-tube (16 x 12) manifold - 316 stainless steel
tubes
Washing speed
Autoclave, Chemical
Dispensing - Syringe Pump
96-/384-well washing:
Dual-Action 96-tube (8 x 12) manifold - 316 stainless steel tubes
1536-well washing:
Two 32-tube (1 x 32) dispense manifolds - sapphire jeweled
316 stainless steel or 316 stainless steel tubes
128-tube (4 x 32) aspiration manifold
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: ±5.0% at 1 µL
Dispense Precision: ≤5.0% CV at 1 µL
≤10.0% CV at 500 nL
Minimum Prime Volume (30” tubing length): 1.20 mL
Dispensing speed
20 µL/well, 96 wells, 1 x 16 tubes: 5.25 seconds
20 µL/well, 384 wells, 1 x 16 tubes: 14 seconds
3 µL/well 1536 wells, 2 x 32 tubes: 7 seconds
Volume range
3 - 3,000 µL/well
Selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Fluid delivery
Two positive displacement syringe drives
Flow rates
1-5
Dispense
accuracy
±1 µL at 5 µL
±1 µL at 20 µL
±1% at 100 µL
Vacuum filtration
Selectable vacuum levels: 0 to -380 mmHg (final at 30 seconds)
Vacuum filtration time range: 5 - 999 seconds
Dispense
precision
≤5% CV at 5 µL
≤2.5% CV at 20 µL
≤1% CV at 100 µL
Volume range
3 - 3,000 µL/well (model dependent)
Selectable in 1 µL increments
Reagent selection
Automatic switching for up to 4 reagents (optional)
Buffer selection
Automatic switching for up to 4 wash buffers (model
dependent)
Supply bottle
1 L or 2 L (model dependent)
Sterilization
Autoclave (model dependent), Chemical
Flow rates
1 - 11, including low flow cell wash rates (model dependent)
Wash cycles
1 - 250
Dispense accuracy
±3%
Dispense precision
≤3% CV (model dependent)
Residual volume
≤2 µL/well
Sterilization
Chemical
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz.
Dimensions
16.5” W x 18” D x 12.5” H (42 x 46 x 32 cm)
Weight
32 lbs (14.5 kg)
Regulatory
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic use
models are available.
Specifications subject to change. Performance values represent the average observedfactory test values.
www.biotek.com
39
405™ Touch Microplate Washer
BioTek’s 405™ Touch Microplate
Washer takes plate washing to the
next level with an enhanced user
interface, increased convenience,
assay applications and automated
maintenance features.
Industry Leading, User Pleasing
The 405 Touch Microplate Washer
incorporates all the features and
functionality of the prior ELx405
models, and improves accessibility
through its touchscreen and
extensive onboard software. 96and 384-well microplate based wash
procedures are only ‘two touches’
away with the easy-to-use interface.
Additionally, two USB flash drives
provide convenient file transfer,
storage and operation. A context
sensitive Help System and several
instructional videos are also included.
The Standard for Automation
The 405 Microplate Washer makes
quick work of any washing assay,
and is especially well suited for
integration into automated systems,
where the wash process is controlled
remotely.
Cell and Bead Assays
The 405 is available in various
models for optimized performance
with the most sensitive and rigorous
assay requirements. When the
protocol calls for washing loosely
adherent cells, the Select model is
fine-tuned with angled dispense
tubes, extra low flow rates and
unique X, Y and Z positioning.
Magnetic and polystyrene bead
washing are effectively accomplished
with the 405.
Typical Applications:
40
u
ELISAs
u
MSD assays
u
HCS immune cytochemistry
u
FLIPR® Ca2+ flux
u
Cell-based assays
u
Magnetic and polystyrene bead assays
u
Gene expression assays
u
Cytokine assays
u
ELISPOT assays
u
Plasmid DNA purification
u
Serum/plasma sample preparation
u
Cell signaling – phospho flow setup for flow cytometry
u
SiLA compliant integration (with LHC software)
Liquid Handling n 405 Touch
Verify™ Technology and
Automated Ultrasonic Cleaning
BioTek’s new patented Verify
technology runs an automated QC
check for manifold tube blockage,
and visually reports any failures.
Patented Ultrasonic Advantage™
can then be used to automatically
clean the manifolds. Together,
these features make the 405 a
self-checking, self-maintaining
microplate washer!
Applications in Deep Well Washing
The ELx405 Select Deep Well
washes 96- and 384-well plates up
to 50 mm tall, and is also compatible
with standard height plates without
any hardware or software changes.
This versatile system is optimal for
labs working in deep well blocks and
standard plates.
Specifications
General
Assays
ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead (model dependent)
• Multiplex assays
• Bead-based ELISA
Filtration-to-waste processes (optional)
Separation
Biomagnetic separation, vacuum filtration
Microplate types
96- and 384-well plates (configuration dependent)
Low profile and standard height
Deep well up to 50 mm (configuration dependent)
Solid and filter bottom (optional)
• Filter pore sizes 0.45 µm to 1.2 µm
Shaking
Programmable in minutes and seconds, up to 60 minutes
Intensities – slow, medium, fast or variable
Soak time
Programmable in minutes and seconds, up to 60 minutes
Magnet
High strength 96- and 384-well designs
Choice of two designs, custom for BioTek microplate
washers:
Flat
• Flat-bottom well - beads pulled to band across
well bottom
• Round-bottom well - beads pulled to button at
well bottom
Ring - beads pulled to 4-zone ring at well bottom
Safety/
convenience/
maintenance
Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Pre-programmed maintenance routines
Automation
BioStack™ Microplate Stacker – up to 50 ANSI/SLAS
standard height plates (optional) (Except filter bottom plates)
Onboard
software/GUI
LED color touch screen
Predefined sample wash protocols
Create or edit multiple custom protocols
Predefined maintenance protocols
Software
Liquid Handling Control™ (LHC), for PC wash protocol
programming and execution (optional). SiLA compliant
driver available.
Interface
For PC control and BioStack™ integration – 1 USB port,
1 9-pin RS232 port
For file transfer – 2 USB Flash Memory ports
Operating
temperature
15 °C to 30 °C
Humidity
10% to 85%, non-condensing
Washing
Manifold types
96-well washing:
96-tube (8 x 12) manifold
96-/384-well washing:
96-tube (8 x 12) Dual-Action™ manifold
384-well washing:
192-tube (16 x 12) Dual-Action manifold
Washing speed
Solid bottom plates:
96-well:
3 aspirate/dispense cycles, 300 µL/well, 96-tube manifold,
final aspirate: ≤30 seconds
384-well:
3 aspirate/dispense cycles, 100 µL/well, 96-tube manifold,
final aspirate: ≤80 seconds
1 aspirate/dispense cycle, 400 µL/well, 192-tube manifold,
final aspirate: ≤20 seconds
Filter bottom plates:
Variable, based on wash parameters
Fluid delivery
One internal positive displacement pump
Vacuum
filtration
Selectable vacuum levels (final at 30 seconds):
Lowest
Low
Medium
High
Highest
-38 mmHg
-113 mmHg
-200 mmHg
-390 mmHg
-506 mmHg
Vacuum filtration time range: 5 to 999 seconds
Volume range
25 to 3,000 µL/well
Selectable in 1 µL increments
Buffer selection
Internal automatic switching for up to 4 wash buffers
(optional)
Flow rates
High flow to low rates, including cell wash rates
(configuration dependent)
Wash cycles
1 to 250
Dispense
precision
96-well washing – 96-tube (8 x 12) manifold:
≤3% CV: 300 µL/well (deionized water with 0.1% Tween 20
and FD&C #1 blue dye), rate 6, absorbance read at 630 nm
and 450 nm reference
384-well washing – 192-tube (16 x 12) manifold:
≤4% CV: 80 µL/well (deionized water with 0.1% Tween 20 and
FD&C #1 blue dye), rate 7, absorbance read at 630 nm and
450 nm reference
Residual volume
Solid bottom plates:
96-well washing – 96-tube (8 x 12) manifold:
≤2 µL/well: 3-cycle wash, 300 µL/well (deionized water with
0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration
height optimized prior to testing
384-well washing – 192-tube (16 x 12) manifold:
≤2 µL/well: 3-cycle wash, 100 µL/well (deionized water with
0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration
height optimized prior to testing
Filter bottom plates:
96-well:
Average increase weight of plate ≤1.2 g after dispensing
300 μL to 0.45 μm plate, filtration 30 seconds, low vacuum,
blotted
384-well:
Average increase weight of plate ≤4.0 g after dispensing 80 μL
to 1.2 μm plate, filtration 10 seconds, low vacuum, blotted
Sterilization
Chemical:
0.5% sodium hypochlorite, 70% isopropyl alcohol or 70%
ethanol solution
Ultrasonic
cleaning
Built-in Ultrasonic Advantage for protein and salt crystal
buildup. Programmable in hours and minutes,
up to 60 minutes (configuration dependent)
Clog detection
Built-in Verify technology detects dispense and aspirate tubes
for blockage (model dependent)
Supply bottle
4 L or 10 L (optional)
Physical Characteristics
Power
100 – 240 Volts AC. 50/60 Hz., ≤8.0 A
Dimensions
14” W x 17” D x 10” H (35.6 x 43.2 x 25.4 cm)
Weight
With internal buffer switching – 36 lbs (16.5 kg)
Without – 30 lbs (13.5 kg)
Regulatory
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic use
models are available.
Specifications subject to change. Performance values represent the average observedfactory test values.
www.biotek.com
41
ELx50™ Microplate Strip Washer
The ELx50™ Microplate Strip
Washer’s compact footprint
conceals a powerhouse of
washing capabilities unsurpassed
in its class. Exceptional dispense
precision and evacuation
efficiency can be utilized for
both 96- and 384-well strip and
plate washing.
Multiple Washers in One
The ELx50 is a 3-in-1 solution
for 96-well plate formats
automating the wash steps of
ELISAs, magnetic bead assays
and polystyrene bead assays. The
ELx50 can also be equipped with
BioTek’s Dual-Action™ manifold
allowing independent control of
dispense and aspiration manifolds
Typical Applications:
42
u
ELISAs
u
Cell-based assays
u
Magnetic bead assays
u
Polystyrene bead assays
u
ELISPOT assays
u
Multiplex assays
Liquid Handling n ELx50
in both 96- and 384-well formats.
As a welcome upgrade from
manual processing, the ELx50
provides an all-inclusive wash
solution offering consistent
performance and unattended
operation.
Syringe Drive Fluid Delivery
As a self-contained and
programmable washer, the ELx50
allows for complete control of
precise fluidic delivery from
the gentle dripping of a simple
squeeze bottle to the full force
of pressure delivery systems.
Comprehensive onboard software
makes creating protocols quick
and intuitive.
Automated Liquid Level Sensing
Liquid Level Alert™ allows the
convenience of continuous
monitoring for both supply and
waste bottles. At the beginning
and end of a wash protocol, the
liquid level is verified to ensure
an adequate buffer remains
to complete a wash. Sufficient
storage capacity in the waste
bottle is also verified.
Specifications
General
Assays
ELISA
Cell-based assays
Magnetic bead, polystyrene bead (model dependent)
• Multiplex assays
• Bead-based ELISA
Filtration-to-waste processes (model dependent)
Protein arrays
Fluid delivery
One positive displacement syringe drive
Vacuum filtration
Selectable vacuum levels (final at 30 seconds):
0.45 µm 96-well plates:
Low
-91 mmHg
Medium
-150 mmHg
High
-313 mmHg
1.2 µm 96-well plates:
Low
-95 mmHg
Medium
-155 mmHg
High
-299 mmHg
Separation
Biomagnetic separation, vacuum filtration
(model dependent)
Microplate types
96- and 384-well plates (model dependent)
Low profile and standard height
Solid and filter bottom (model dependent)
• Filter pore sizes 0.45 µm to 1.2 µm
Volume range
25 - 3,000 µL/well (configuration dependent)
Selectable in 1 µL increments
Shaking
Programmable in minutes and seconds up to 60 minutes
Intensities - 1-5 (15-19 cycles/second)
Buffer selection
Automatic switching for up to 3 wash buffers
(configuration dependent)
Soak time
Programmable in minutes and seconds up to 60 minutes
Flow rates
1-9
Magnet
High strength 96-well format
Flat
• Flat-bottom well - beads pulled to band across well
bottom
• Round-bottom well - beads pulled to button at well
bottom
Ring - beads pulled to 4-zone ring at well bottom
Wash cycles
1 - 10
Dispense
precision
≤3% CV
Residual volume
Solid bottom plates:
≤2 µL/well
Safety/
convenience/
maintenance
Waste sensing, fluid detection (optional)
Aerosol cover
Overflow protection
Pre-programmed maintenance routines
Onboard
software
Create, edit or run multiple protocols
Vacuum filtration time range: 1 - 180 seconds
Filter bottom plates:
Average increase weight of plate ≤1.2 g after dispensing
300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum,
blotted
Sterilization
Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium
hypochlorite solution
Supply bottle
2L
Washing
Manifold types
96-well washing:
8-tube (1x8) manifold
12-tube (1x12) manifold
96-/384-well washing:
Dual-Action 16-tube (1x16) manifold
Washing speed
Solid bottom plates:
3 asp./disp. cycles: >300 µL/well, 96 wells, 8-tube
manifold, final aspirate: ≤130 seconds
Filter bottom plates:
Variable, based on wash parameters
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz.
Dimensions
14”W x 16”D x 6.5”H (35.6 x 40.6 x 16.5 cm)
Weight
22 lbs (9.8 kg)
Regulatory
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic
use models are available.
Specifications subject to change. Performance values represent the average observedfactory test values.
www.biotek.com
43
Washer Comparison Chart
Which Washer is right for you?
EL406™
MultiFlo™ FX
ELISA
•
•
Cell-based assays
•
•
Magnetic bead assays
•
•
Polystyrene bead assays
•
Filtration-to-waste processes
•
Key Features
Touchscreen user interface
•
USB ports for protocol transfer
•
Performance Specifications
Washing speed: 3 aspirate/dispense cycles,
96-well solid bottom plate, 300 µL/well
≤30 sec
≤130 sec
Dispense precision
≤3% CV
≤3% CV
≤2 µL/well
≤2 µL/well
Residual volume: solid bottom plate
Vacuum filtration: 1.2 µm 96-well plate
0 mmHg to -380 mmHg
General Specifications
Microplate types
96, 384 and 1536
6, 12, 24, 48, 96 and 384
Low profile and standard height
•
•
Solid and filter bottom
•
Deep well
Manifold
6-, 12-, 24-, 48-well washing
96-well washing
Custom manifolds available
96-tube (8x12)
96-/384-well washing
Dual-Action 96-tube (8x12)
384-well washing
Dual-Action 192-tube (16x12)
1536-well washing
Dispense: Two 32-tube (1x32)
Aspiration: 128-tube (4x32)
Ultrasonic Advantage™
•
Dual-Action 8-tube (1x8)
Verify technology
™
Automation ready/BioStack™ compatible
Automatic buffer switching
Flow rates
•
Up to 4
High flow rates to low
cell wash rates
High flow rates to low rates
3 - 3000 µL/well
25 - 30,000 µL/well
Microplate shaking
•
•
Fluid and waste detection
•
(optional)
Flow and vacuum detection
•
Overflow protection
•
•
Pre-programmed maintenance routines
•
•
Onboard software included
•
•
Liquid Handling Control Software compatible
•
•
Volume range
™
44
•
Liquid Handling n Washer Comparison Chart
405™ Touch
405 LS
ELx405™ Select
ELx50™
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
≤30 sec
≤30 sec
≤30 sec
≤130 sec
≤3% CV
≤3% CV
≤3% CV
≤3% CV
≤2 μL/well
≤2 μL/well
≤2 μL/well
≤2 µL/well
-38 mmHg to -506 mmHg
-38 mmHg to -506 mmHg
96 and 384
96 and 384
96 and 384
96 and 384
•
•
•
•
•
•
-95 mmHg to -299 mmHg
•
•
96-tube (8x12)
96-tube (8x12)
8-tube (1x8) or 12-tube (1x12)
Dual-Action 96-tube (8x12)
Dual-Action 96-tube (8x12)
Dual-Action 192-tube (16x12)
Dual-Action 192-tube (16x12)
•
•
•
•
•
•
•
•
•
Up to 4
Up to 4
Up to 4
Up to 3
High flow rates to low
cell wash rates
High flow rates to low
cell wash rates
High flow rates to low
cell wash rates
High flow rates to low
cell wash rates
25 - 3000 μL/well
25 - 3000 μL/well
50 - 3000 μL/well
25 - 3000 µL/well
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Dual-Action 96-tube (8x12)
Dual-Action 16-tube (1x16)
•
www.biotek.com
45
MultiFlo™ FX Multi-Mode Dispenser
The new MultiFlo™ FX MultiMode Dispenser offers modular
configurations of up to four
independent dispensers and a
microplate washer in one compact
platform. A color touchscreen
interface makes programming
quick and easy.
Multi-Mode Dispensing
MultiFlo FX becomes a versatile
multi-mode dispenser with the
addition of either the RAD™
technology for random access
dispensing to 6- to 384-well plates
or a wash module for 6- to 384well plate washing. Fast, intuitive
programming and operation are via
the color touchscreen user interface.
A MultiFlo FX configured with either
RAD™ technology or the wash
module replaces up to five liquid
handlers.
Parallel Dispense™ Technologies
Offering BioTek’s unique
combination of peristaltic and
microprocessor controlled syringe
pump dispensing, the MultiFlo FX
enables users to choose which is
best for a specific reagent. While
peristaltic pumps offer low prime
volumes and backflush capabilities,
BioTek’s syringe drives are programand-forget solutions that never
require recalibration. Automated
dispensing with walk-away
confidence.
Modular and Upgradable
The MultiFlo FX is configurable
and upgradable from dispense or
wash only, to a combined dispense
and wash combination, with 1-to-4
reagent dispensing with peristaltic
and/or syringe driven precision
dispensers, or the addition of a
RAD module for single channel and
high volume dispensing. Purchase
the modules required now, and
Typical Applications:
XXCell-based
XXPrimary
assays
and secondary screening assays
XXDispense/wash
XXCompound
XXGenomics
XXMagnetic
protocol automation
storage
and proteomics research
bead assays
XXELISAs
XXMultiplex
assays
XXAutomated
46
Liquid Handling n MultiFlo FX
cell washing, fixing and staining for cellular imaging
upgrade in the future as assay
needs change. With its compact
footprint and base height of less
than 8 inches, the MultiFlo FX
comfortably fits on any lab bench
or robotic system. The MultiFlo FX
is easily integrated with a BioStack™
Microplate Stacker for walk-away
automation.
Versatile Applications - Liquid
Handling
A wide array of plate type settings
accommodates 6- to 1536-well
plate formats up to 50 mm high for
dispensing. Volumes from 500 nL μL
to 3 mL are dispensed with accuracy
and precision. The wash module
works with 6- to 384-well plates in
standard, half and deep well, and
with cluster or mini-tubes. Automate
cell-based assays by integrating
MultiFlo FX with the new BioStack™
4 to handle lidded plates with speed
and ease.
Specifications
General
User interface
5.7” LCD touch screen display
USB ports (2)
For protocol storage/transfer and for optional external mouse or
keyboard
Shaking
Slow, medium, fast or variable up to 60 minutes
Soak time
Programmable in minutes and seconds, up to 60 minutes
Safety/
convenience/
maintenance
Adjustment utility for plate positioning
Pre-programmed maintenance routines
Automation
Compatible with BioStack and 3rd party automation
Onboard software
Create, edit or run multiple protocols, extensive Help system
Software
Liquid Handling Control™, for PC protocol programming and
execution (optional)
Labware types
Multi-channel peristaltic and syringe dispensing: 6-, to 1536-well
microplates; low profile, standard height and deep well formats,
PCR trays, microtubes. Max. height: 47 or 50 mm (carrier dependent)
RAD dispensing: Single tip; 6-, to 384-well plates; low profile,
standard height and deep well formats; PCR trays and microtubes.
8-to-1 tip; 6-, 12-, and 24-well plates
Washing: 6- to 384-well standard plates (with appropriate manifold)
Sterilization
Peristaltic and syringe pump dispensers: Autoclave, up to 50 cycles
at 121° C and 1 bar (750 mmHg) or chemical, 70% ethyl or isopropyl
alcohol and 0.5% sodium hypochlorite solution
Washer: Chemical, 70% ethyl or isopropyl alcohol and 0.5% sodium
hypochlorite solution
Dispensing – Peristaltic Pump (Multi-Channel)
Manifold types
1 x 8 - sapphire jeweled 316 SS, 316 SS or polypropylene tips, with
1, 5 or 10 µL tubing
Dispense speed
(cassette, volume)
1 µL cass, 1 µL/ well: 6s (384 wells), 21s (1536 wells)
5 µL cass, 5 µL/ well: 6.5s (384 wells)
5 µL cass, 10 µL/ well: 3s (96 wells)
5 µL cass, 20 µL/ well: 3.5s (96 wells)
10 µL cass, 10 µL/ well: 8s (384 wells)
Volume range
500 nL - 3,000 µL/well, selectable in 1 µL increments
Fluid delivery
One or two positive displacement peristaltic pumps
Flow rates
Adjustable high flow to very low flow rates
Minimum prime
volume (cassette,
tube length)
1 µL cass, 18”: 0.78 mL; 1 µL cass, 30”: 1.20 mL
5 µL cass, 18”: 2.75 mL; 1 µL cass, 30”: 4.23 mL
10 µL cass, 18”: 4.79 mL; 10 µL cass, 30”: 7.36 mL
Dispense
performance
(cassette, volume
range)
1 µL cass, 1-50 µL: Accuracy ≤5% at 1 µL, Precision ≤5% CV at 1 µL
1 µL cass, 1-50 µL: Precision < 10% CV at 500 nL
5 µL cass, 5-2,500 µL: Accuracy ±2% at 5 µL, Precision ≤2.5% CV at 5 µL
10 µL cass, 10-3,000 µL: Accuracy ±2% at 10 µL, Precision ≤2% CV at
10 µL
Recommended
replacement
interval
1 µL Cassette: 1,000 384-well microplates at 5 µL/well
5 µL Cassette: 1,000 96-well microplates at 50 µL/well
10 µL Cassette: 1,000 96-well microplates at 100 µL/well
Dispensing – Peristaltic Pump (RAD Technology)
Manifold types
RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle
RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute
Volume range
500 nL – 30,000 µL
Minimum prime
volume (cassette,
tube length)
1 µL cass, 18”: 90 µL ; 1 µL cass, 30”: 150 µL
5 µL cass, 18”: 320 µL; 1 µL cass, 30”: 530 µL
10 µL cass, 18”: 555 µL; 10 µL cass, 30”: 920 µL
Dispense speed
(high flow)
(cassette, volume)
1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells)
1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells)
5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells)
5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells)
10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells)
10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells)
Dispense
performance
(cassette, flow
rate, volume)
1 µL cass (med), 0.5 µL/well: Precision 10% CV
1 µL cass (med), 1 µL/well: Accuracy ± 10%, Precision 10% CV
1 µL cass (med), ≥2 µL/well: Accuracy ± 5%, Precision 5% CV
5 µL cass (high), 5 µL/well: Accuracy ± 4%, Precision 5% CV
5 µL cass (high), ≥10 µL/well: Accuracy ± 2%, Precision 2.5% CV
10 µL cass (high), 10 µL/well: Accuracy ± 4%,Precision 4% CV
10 µL cass (high), ≥20 µL/well: Accuracy ± 2%, Precision 2 %CV
8-to 1 cass(high), 40 µL/well: Accuracy ± 4%
8-to 1 cass (high), ≥10 µL/well: Precision 2.5%CV
8- to 1cass(high), ≥80 µL/well: Accuracy ± 2%
Dispensing – Syringe Pump
Manifold types
96-/384-well dispensing: (1) 2 x 8 manifold, 316 SS tubes,
autoclavable and non-autoclavable available. (2) 1 x 16 manifolds,
316 SS tubes, autoclavable
1536-well dispensing: (2) 1 x 32 manifolds – sapphire jeweled 316
SS SB or 316 SS LB tubes, autoclavable
6-, to 48-well dispensing: Custom autoclavable manifolds available
for each plate type
Dispensing speed
20 µL /well, 96 wells, 1x16: 5 seconds
20 µL /well, 384 wells, 1x16: 14 seconds
3 µL /well, 1536 wells, 2x32: 7 seconds
Volume range
3-3,000 µL/well (manifold dependent) Selectable in 1 µL increments
Fluid delivery
Two positive displacement syringe drives
Dispense
performance
(96- and 384-well)
5 µL/well: Accuracy ± 1 µL, Precision ≤ 5% CV
20 µL/well: Accuracy ± 1µL, Precision ≤ 2.5% CV
100 µL/well: Accuracy ± 1 %, Precision ≤ 1% CV
Minimum prime
volume
12 mL
Supply bottle
volume
1 L or 2 L (model dependent)
Washing
Manifold types
96-/384-well plate washing: 1x8 manifold;
6-, 12-, 24-, 48-well plate washing: Custom manifolds available
Washing speed
3 asp. /disp. cycles: >300 µL/well, 96 wells, 8-tube manifold, final
aspirate ≤130 seconds
Fluid delivery
One positive displacement syringe pump
Volume range
20-30,000 µL/well
Flow rates
140-422 µL/well/second
Wash cycles
1-10
Dispense precision
≤3% CV (96-/384-well plates; 300 µL/well) and (12-/48-well plates;
volume dependent)
≤5% CV (6-well plates; 5560 µL/well)
Dispense accuracy
±3%
Residual volume
≤2 µL/well, 300 µL dispense, 0.1% Tween
Supply bottle
volume
2L
Physical Characteristics
Power
100 - 240 Volts AC.
Dimensions
Base instrument:
17.19””W x 11.75” D x 8” H (43.51 x 29.21 x 20.32 cm)
Weight
Base instrument:
19.5 lbs (8.8 kg)
50/60 Hz.
Regulatory
Regulatory
CE and TUV marked, RoHS Compliant. Models for In Vitro
Diagnostic use are available.
Specifications subject to change. Performance values represent the average observedfactory test values.
www.biotek.com
47
MicroFill™ Microplate Dispenser
With its microprocessorcontrolled syringe drive
technology, the MicroFill™
Microplate Dispenser provides
outstanding accuracy and
precision while dispensing into
24-, 96- and 384-well plates.
Low Maintenance Design
The MicroFill is an economical,
compact and reliable alternative
to conventional microplate
dispensers. Its microprocessorcontrolled syringe pump
provides optimal dispense
performance without timeconsuming recalibration, cassette
replacement and maintenance.
Syringes are ideal for higher
volume filling, with noteworthy
speed improvements compared to
other dispense technologies.
Guaranteed Sterility
Unattended Operation
The entire fluid path is
autoclavable for applications
requiring sterility. The MicroFill’s
pump, tubing, dispense manifold
and supply bottle are quickly
changed for no reagent carryover.
User-controlled dispense flow
rates allow low- to high-velocity
dispensing for both biochemical
and cell-based assays. Lowprofile, standard and deep well
microplates are all accommodated
with a broad volume range from
5 µL to 6 mL.
For increased throughput, the
MicroFill can be integrated with
BioTek’s BioStack™ Microplate
Stacker or interfaced to third
party automated systems with
its available interface software.
MicroFill drivers are available from
most of today’s leading system
providers.
Typical Applications:
XXPrimary
and secondary screening assays
XXCompound
XXGenomics
and proteomics research
XXCell-based
XXELISAs
48
Liquid Handling n MicroFill
storage
assays
Specifications
General
Number of reagents
1
Microplate types
24-, 96- and 384-well plates in low profile, standard and deep well formats
PCR plates
Microtubes
Soak time
Programmable in minutes and seconds, up to 60 minutes
Safety/convenience/maintenance
Pre-programmed maintenance routines
Automation
Compatible with BioStack™ and 3rd party automation
Onboard software
Create, edit or run multiple protocols
Software
Interface software (optional) for robotic system integration
Dispensing - Syringe Pump
Dispensing speed
10 µL/well, 96 wells, 1x16: 4 sec
5 µL/well, 384 wells, 1x16: 7 sec
Manifold type
24-well dispensing:
One 8-tube (1 x 8) manifold – 316 stainless steel tubes
96-well dispensing:
One 8-tube (1 x 8) manifold – 316 stainless steel tubes
96-/384-well dispensing:
One 16-tube (1 x 16) manifold – 316 stainless steel tubes
Volume range
5 - 6,000 µL/well (manifold dependent)
Fluid delivery
One positive displacement syringe drive
Flow rates
1-5
Dispense accuracy
±1 µL at 5 µL
±1 µL at 20 µL
±1% at 100 µL
Dispense precision
≤5% CV at 5 µL
≤2.5% CV at 20 µL
≤1% CV at 100 µL
Minimum prime volume
10 mL
Supply bottle
1L
Sterilization
Autoclave:
Up to 50 cycles at temperatures and pressures of 121 °C and 1 bar (750 mmHg)
Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Physical Characteristics
Power
100 - 240 Volts AC.
Dimensions
15” W x 18” D x 7”H (38 x 46 x 18 cm)
Weight
20 lbs (8.9 kg)
50/60 Hz.
Regulatory
Regulatory
CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
Specifications subject to change. Performance values represent the average observed factory test values.
www.biotek.com
49
Dispenser Comparison Chart
Which Dispenser is right for you?
EL406™
Key Features
ELISA
•
Cell-based assays
•
Number of reagents
1 to 3
Dispense technology
Peristaltic and/or Syringe
Fully modular and upgradable
•
Performance Specifications
Dispensing speed
Peristaltic pump (8-tip, 1x8)
96-well, 10 µL/well; 384-well, 5 µL/well
3 sec; 6 sec
Syringe pump (16-tube, 1x16)
96-well, 20 µL/well; 384-well, 20 µL/well
5.25 sec; 14 sec
Dispense accuracy - typical at 5 µL
Peristaltic pump
±2%
Syringe pump
±1 µL
Dispense precision - typical at 5 µL
Peristaltic pump
≤2.5% CV
Syringe pump
≤5% CV
General Specifications
Microplate types
96, 384 and 1536
Low profile and standard height
•
Deep well
Strips and full plates
•
Cassette/manifold
RAD cassettes
Peristaltic pump
8-tip (1x8)
Syringe pump, 6-well to 384-well dispensing
Syringe pump, 96-well to 1536-well dispensing
•
Variable flow rates
•
Volume range
50
8-tube (1x8)
Automation ready/BioStack compatible
™
1 - 3,000 µL/well
Microplate shaking
•
Autoclavable fluid path
•
Onboard software included
•
Liquid Handling Control™ Software compatible
•
Liquid Handling n Dispenser Comparison Chart
MultiFlo™ FX
MicroFill™
•
•
•
•
1 to 4
1
Peristaltic and/or Syringe
Syringe
•
3 sec; 6 sec
5.25 sec; 14 sec
4 sec; 7 sec
±2%
±1 µL
±1 µL
≤2.5% CV
≤5% CV
≤5% CV
6 to 1536
24, 96 and 384
•
•
•
•
•
•
•
8-tip (1x8)
•
8-tube (1x8)
8-tube (1x8)
•
•
•
1 - 30,000 µL/well
5 - 6,000 µL/well
•
•
•
•
•
•
www.biotek.com
51
Liquid Handling Control™ Software
Liquid Handling Control™ (LHC™)
Software allows MultiFlo™ FX
Dispenser, EL406™ Washer
Dispenser and 405™ Touch and
405 LS Washer users the
convenience of programming
important assay-specific
protocol requirements in a
Windows® environment.
Expanded Versatility
LHC Software is a powerful yet
flexible interface for use with
BioTek’s microplate dispensers
and washers. Any programming
sequence possible onboard the
liquid handler may be duplicated
from the computer with LHC
Software. LHC also allows a
virtually unlimited number of
methods to be linked together for
the most complex liquid handling
routines. From a washer’s first
prime routine, multiple microplate
processes over time, ultrasonic
cleaning to dissolve protein or salt
crystal build-up to a final system
rinse, LHC Software enables
unattended operation.
21 CFR Part 11 Compliance
Safe Record Keeping
To meet the demands of the GxP
laboratory, LHC Secure offers
features to ensure compliance to
21 CFR Part 11. Flexible multi-user
permission levels and electronic
protocol and system audit trail
signing are all available whenever
additional security is required.
Protocol parameters may be
quickly printed for safe record
keeping. Alternatively, onboard
instrument protocols may be
uploaded and backed up on a
laboratory’s network. Satellite
research labs working on joint
projects can be certain their
wash parameters are identical for
experimental integrity.
Custom Maintenance Reminders
To facilitate maintenance and keep
a washer or dispenser in peak
condition, factory recommended
maintenance procedure reminders
can be preset and customized
appropriately for a busy
laboratory’s usage and throughput
requirements. LHC also supports
BioStack™ Microplate Stacker
integrations.
Intuitive StepWise™ protocol creation for ultimate flexibility
52
Liquid Handling n Liquid Handling Control Software
SiLA Compliant Drivers
For automated systems that
require SiLA compliant integration,
LHC SiLA is available.
Washer & Dispenser Accessories
BioTek offers a wide range of accessories to help increase productivity, expand your plate washer’s and dispenser’s
capabilities, and maintain the performance of your BioTek microplate liquid handling system. See our web site for a
complete listing of available accessories.
Dispense/Waste Systems
Peristaltic Pump Dispenser
Cassettes
A wide selection of peristaltic pump
cassettes are available with choices
in volume ranges, tip materials and
bore sizes for use with EL406™ and
MultiFlo™ FX.
A dispense/waste system is
required on all 405™ Touch,
405 LS and EL406 models. Many
selections are available based
on throughput, bottle size and
vacuum pump requirements.
Syringe Pump Dispenser Manifolds
A range of MultiFlo FX and
MicroFill™ dispense manifolds are
available for various microplate
types and reagent characteristics.
BioStack™ Microplate Stacker
Automate routine processes with
this compact stacker.
Instrument Qualification
See the Compliance Section on
pages 62-63 for details about
BioTek’s product qualification
tools and services.
3-Instrument Rack
For third party robotic system
integration, a rack is available for
supporting up to three dispensers
or other BioTek instrumentation.
www.biotek.com
A searchable Accessory database, including descriptions and photos, is available on www.biotek.com
53
Precision™ Microplate Pipetting Systems
The Precision™ is an innovative
solution for automated liquid
handling. With its ability to
perform virtually any routine
liquid transfer, Precision replaces
tedious manual pipetting.
Automate Manual Pipetting
The Precision can be customized
with a range of options perfect
for medium throughput labs
looking to automate their
everyday pipetting with walk-away
confidence. BioTek’s proprietary
pipette technology and unique tip
sealing allow most standard tips to
be used for transfers in common
sample tubes and 6- to 384-well
microplate formats.
Open Deck Layout and Flexible
Software
Space Saving, Compact
Footprint
A user-configurable, multistation deck allows for flexible
experimental design; microplates,
tips and other labware may be
placed in nearly any location
for optimal efficiency. Available
Precision Power™ Software offers
complete Precision control
with intuitive protocol creation,
expanding the instrument’s
dynamic capabilities with a
graphical program simulator and
sample ID tracking.
Its small footprint and wellorganized design make the
Precision ideally suited for
installation inside standard
size biological safety cabinets
and chemical fume hoods. The
Precision XS model delivers
outstanding liquid handling
performance with four liquid
transfer tools on a single platform.
All four may be intermixed
throughout a fully automated
protocol – single and multichannel pipetting along with
single- and multi-channel bulk
reagent dispensing.
Typical Applications:
54
u
Sample transfers from tube to microplate
u
Serial dilutions
u
Mixing
u
Plate replication - mother/daughter transfers
u
Reagent addition
u
Hit picking
u
ELISA automation
u
Secondary screening assays
u
Compound profiling
u
Cell-based assays
Liquid Handling n Precision
Specifications
Precision XS
Precision
General
General
Microplate types
6- to 384-well plates, any test tube ≤100 mm
Microplate types
96- and 384-well plates
Automation
Compatible with BioStack™ and 3rd party automation
Automation
Platform
6 stations
Compatible with BioStack (excluding 1x12 pipetting) and
3rd party automation
Software
Precision Power™, for PC control programming and execution
(included)
Platform
1x8:
6 stations
1x12:
4 stations
Pipetting
Manifold types
1x8, single-channel
Onboard software
Create, edit or run multiple protocols
Pipetting speed
1x8:
100 µL/well, 96 wells, tip change: 3 min
Software
Precision Power, for PC control programming and execution
(optional)
Single-channel:
100 µL/well, 96 wells, tip change: 22 min
Pipetting
Manifold types
1x8, 1x12 (configuration dependent)
Pipetting speed
Single-channel:
5 - 200 µL
1x8:
100 µL/well, 96 wells, tip change: 3 min
Volume range
5 - 120 µL
Air displacement syringe drives
Fluid delivery
Air displacement syringe drives
Dispense accuracy
±1% at 100 µL
Dispense accuracy
±1% at 100 µL
Dispense precision
≤1.5% CV at 100 µL
Dispense precision
≤1.5% CV at 100 µL
Pipette tips
BioTek and other commercially available tips
Pipette tips
BioTek and other commercially available tips
Volume range
Fluid delivery
1x8:
5 - 120 µL
Dispensing - Syringe pump
Manifold types
Dispensing speed
Volume range
Dispensing - Syringe pump
96-/384-well dispensing:
One 8-tube (1x8) manifold - 316 stainless steel tubes
Manifold types
One 8-tube (1x8) and/or 12-tube (1x12) manifold - 316 stainless
steel tubes
6- to 384-well dispensing:
One single-channel probe
Dispensing speed
100 µL/well, 96 wells, 1x8: 14 seconds
Volume range
10 µL - 10 mL
100 µL well, 96 wells, 1x8: 14 seconds
100 µL well, 96 wells, single-channel: 4 minutes
Fluid delivery
One positive displacement syringe drive
Dispense accuracy
±1% at100 µL
Dispense precision
≤1.5% CV at 100 µL
Supply bottle
volume
2L
1x8:
10 µL - 10 mL
Single-channel:
5 µL - 10 mL
Physical Characteristics
Fluid delivery
One positive displacement syringe drive
Dispense accuracy
±1% at 100 µL
Power
100 - 240 Volts AC.
Dispense precision
<1.5% CV at 100 µL
Dimensions
Supply bottle
volume
1x8:
2L
Instrument only:
21” W x 15” D x 16” H
(525 x 374 x 400 mm)
Instrument with optional cabinet:
23” W x 17.5” D x 17.5” H
(575 x 435 x 435 mm)
Single-channel:
125 mL
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz.
Dimensions
Instrument only:
25” W x 16” D x 20” H
(640 x 410 x 510 mm )
50/60 Hz.
Weight
Instrument only: 28 lbs (12.7 kg)
Instrument with optional cabinet: 38 lbs (17.2 kg)
Regulatory
Regulatory
CE and TUV marked. In Vitro Diagnostic use models are available.
Instrument with optional cabinet:
30” W x 20” D x 22” H
(760 x 510 x 560 mm)
Weight
Regulatory
Instrument only: 40 lbs (18 kg)
Instrument with optional cabinet: 64 lbs (29 kg)
CE and TUV marked. In Vitro Diagnostic use models are available.
Specifications subject to change. Performance values represent the average observed
factory test values.
www.biotek.com
55
56
Robotics n Overview
Robotics
Automation products provide speed, flexibility
and unattended operation when configured with
BioTek’s line of microplate imagers, readers, washers
and dispensers. The result is a scalable, costeffective system that can adapt to your changing
requirements. The latest innovation in BioTek’s
BioStack™ automation products is plate de-lidding
capability, ideal for cell-based assays and larger
volume microplate compatibility to extend BioStack’s
applications utility.
www.biotek.com
57
BioStack™ Microplate Stacker
BioStack™ is a compact and
versatile microplate stacker
compatible with BioTek’s
microplate washers, dispensers,
pipetting, detection and
imaging systems. BioStack is
easy to use and provides walkaway automation for routine
processes, including processes
requiring plate de-lidding and
re-lidding.
Ultra Fast Transfer Speeds
BioStack offers the fastest plate
transfer time, taking less than
10 seconds to remove and
replace plates on the instrument
carrier. BioStack is well-suited for
high throughput plate stacking
requirements with BioTek readers,
washers and dispensers.
Plate De-lidding
Many cell-based microplate
processes require lidded plates
during incubation and to protect
sterility. Typically, automation of
these processes meant purchasing
an expensive microplate handler to
de-lid the plates for measurement
or liquid handling operations.
BioStack now offers an affordable
option for plate de-lidding in the
BioStack 4 model to interface
with BioTek’s detection and liquid
handling instruments.
Multiple Microplate
Geometry Compatible
BioStack is compatible with
standard 96- and 384-well
plates, low volume 384-well
plates and 1536-well plates. The
BioStack 4 adds 24- and 48-well
plates to its menu of compatible
microplate labware, providing
higher throughput in a walk-away
system for a variety of microplate
geometries. An available barcode
scanner provides additional
automation for high-throughput
plate processing.
Typical Applications:
58
u
Cell-based assays
u
ELISAs
u
Primary screening assays
u
Colorimetric, fluorometric and luminescent assays
Robotics n BioStack
Plate IDs are read and sent to the
plate data file in Gen5™ or LHC™
Secure software for storage or
export.
10-, 30- or 50-Microplate Stacks
Choose between 10-, 30- or
50-plate stacks to best suit your
throughput requirements. Low
volume, half-height plates are also
compatible, with up to 75-plates
capacity in the 50-plate stack.
Compact, Rugged Design
BioStack allows worry-free
operation, even under the heaviest
usage. The motors, mechanical
assemblies and software are all
designed for long term, continuous
use and maintenance-free use. The
rotational gripper and very small
footprint allows for integration
position versatility and for optimal
fit within a biosafety enclosure or
for space-savings on the benchtop.
Specifications
BioStack 4
BioStack 3
BioStack
General
Microplate types
ANSI/SLAS Standard and low profile 96-,
384- and 1536-well plates
24- and 48-well plates (model dependent)
Maximum plate height 23.2 mm
ANSI/SLAS Standard and low profile 96-,
384- and 1536-well plates
Maximum plate height 14.6 mm
ANSI/SLAS Standard and low profile 96-,
384- and 1536-well plates
Maximum plate height 14.6 mm
Lidded plate handling
De-lidding capability: (lids always removed
during processing) 96-,384-, and 1536-well
plates. Maximum height, including lids:16.9 mm
n/a
n/a
10-, 30- and 50-plate stacks are removable
and interchangeable
10-, 30- and 50-plate stacks are removable
and interchangeable
96-/384-well plates: Up to 50 plates
1536-well plates: Up to 75 plates
96-/384-well plates: Up to 50 plates
1536-well plates: Up to 75 plates
Nunc plates: (lids can remain on plate during
process, or can be removed) 6-, 12-, 24-, 48well plates.
Maximum height, including lids: 23.2 mm
Microplate capacity
10 and 30 plate stacks are removable and
interchangeable (50-plate stacks may be used
with non-lidded plates only)
96-/384-well plates: Up to 30 plates
1536-well plates: Up to 75 plates
Barcode scanner
(option)
Landscape or portrait orientation, Code 39,
Codabar, UPC/EAN, Code 128 compatible
Landscape or portrait orientation, Code 39,
Codabar, UPC/EAN, Code 128 compatible
Landscape or portrait orientation, Code 39,
Codabar, UPC/EAN, Code 128 compatible
Processing speed
(plate exchange time)
<20 seconds (with de-lidding)
<12 seconds (without lids)
<10 seconds
<33 seconds
Direct control
Washers and dispensers with keypad
interface can directly control BioStack
Washers and dispensers with keypad
interface can directly control BioStack
Washers and dispensers with keypad
interface can directly control BioStack
PC software
LHC2 for liquid handling instruments (optional)
LHC2 for liquid handling instruments
(optional)
LHC2 for liquid handling instruments
(optional)
Gen5 2.0 for readers
Gen5 2.0 for readers
Gen5 2.0 for readers
Physical Characteristics
Power
100 - 240 Volts AC. 50/60 Hz.
100 - 240 Volts AC. 50/60 Hz.
100 - 240 Volts AC. 50/60 Hz.
Weight
<25 lbs (11.3 kg)
<25 lbs (11.3 kg)
<25 lbs (11.3 kg)
Dimensions
8.3” W x 22” D (21 x 56 cm)
7.4” W x 20.7” D (18.8 x 52.6 cm)
7” W x 18.5” D (18 x 47 cm)
Overall height will vary depending on
connected instruments and stacks used
Overall height will vary depending on
connected instruments and stacks used
Overall height will vary depending on
connected instruments and stacks used
CE and TUV marked. ROHS Compliant.
CE and TUV marked. ROHS Compliant.
CE and TUV marked. ROHS Compliant.
Regulatory
Regulatory
Specifications subject to change. Performance values represent the average observed factory test values.
www.biotek.com
59
60
Service & Support n Overview
Service
& Support
Our teams are committed to providing the service and
support you need to sustain the optimal performance
of your BioTek products. BioTek Service Engineers
provide personal support for instrumentation, software,
parts and applications at our Global Technical Support
Center. BioTek Scientists, Engineers, Technicians and
Sales Representatives provide valuable assistance to
laboratories worldwide.
www.biotek.com
61
Compliance
As an ISO certified manufacturer, BioTek understands the importance of standardized product qualification
procedures and traceability and provides a number of tools and services designed to streamline the process and
minimize the resources required to perform such testing.
21 CFR Part 11
Compliance Products
Product Qualification
Gen5™ Secure Data Analysis
Software Validation
Software for microplate readers and
A Validation Package is available for
LHC™ Secure protocol definition
Gen5 Software to allow testing and
and control software for BioTek’s
validation of key functions within
microplate washers and dispensers
Gen5 and Gen5 Secure. Included in
are uniquely designed to help
the easy-to-use package are:
ensure compliance to 21 CFR
u
Test Plans
Part 11. Both software programs
u
Results Checklists
offer important security features,
u
Data sets
including:
Electronic signature of data and protocol files
u
u
Secure data storage
Multiple and definable user permission levels
u
Instrument IQ/OQ/PQ Packages
BioTek offers a complete menu of
Product Qualification Packages for
all of our microplate instruments.
All product Qualification Packages
u
Data and protocol audit trails
are fully validated to assure that
u
Protected functions
the procedures and associated
data/spreadsheets supplied in
IVD Compliance
the package meet regulatory
Many BioTek microplate instruments
requirements. Within each package,
are labeled for In Vitro Diagnostic
you’ll find detailed:
use, identified by the IVD logo.
u
Product Specifications
Other products may have IVD
u
Qualification interval guidelines
Compliant models
available. Contact
IQ/OQ/PQ test plans and procedures
u
Data sets (where applicable)
CustomerCare at
u
CustomerCare@biotek.com
u Qualification checklists and log
sheets for complete documentation
for more information.
RoHS2 Directive 2011/65/EU
BioTek is committed to helping
protect the environment in all of
our customers’ countries. BioTek
products that meet the RoHS
directive are indicated in the
Regulatory section of the product
specifications in this catalog.
62
Service & Support n Compliance
Test Plates
The use of standardized plates to
Fluorescence Test Plates
supplement the verification of an
Ideal for quick checks of the fluorescence
instrument’s performance is a time-
intensity detection system between
and resource-saver in most laboratory
more thorough instrument qualification.
environments. BioTek offers several test
The Fluorescence Test Plate aids
plates to facilitate the test procedures
in maintaining GxP compliance by
found in our microplate reader IQ/OQ/PQ
automatically checking a series of critical
packages, and can be automated through
performance parameters, including:
the Gen5 software.
u
Alignment
u
Cross talk
Absorbance Test Plates
u
Signal-to-noise ratio
For use with the ELx800, ELx808,
u
Linearity
Epoch 2, Epoch, PowerWave HT,
u
Precision
Synergy and Cytation multi-mode
reader absorbance modes. Ensure GxP
Luminescence Test Plates
compliance by checking instrument
This NIST-traceable Luminescence Test
performance against specifications for:
Plate is used with the applicable Product
u
Accuracy
Qualification Package or updated User’s
u
Repeatability
Manual. Features include:
u
Linearity
u
u
Wavelength accuracy (for
monochromator-based systems)
NIST-traceability certificate
guarantees a controlled light output
from the test plate
u
Simple design, easy to use: just turn the plate on, and read the ultra-stable, low
light level LEDs
Test Plate Recertification Programs
are available. Contact BioTek Service
for details. www.biotek.com/contact
www.biotek.com
63
Applications Support
At BioTek, our customers’ applications come first. Whether it’s our existing or new products in development, we
design in capabilities to enable your most important applications. To facilitate this process, BioTek has an on-site
Applications Lab with a team of seasoned scientists continuously working on cutting-edge scientific applications
and partnering with the best-known reagent and consumable vendors. Below are some examples of hot
application areas where we have demonstrated the utility of our products.
XXMitochondrial
oxidative stress and
apoptosis
XXAutophagy
and lysosomal
disorder assays
Live Cell Assays
XXRNA
quantification using
fluorescent nanoprobes
XXMultiplexed
3D Cell Culture
second messenger
assays (Ca2+, cAMP, diacylglycerol,
PIP2) using genetically engineered
probes
XXSignal
XXCell
XXLong
XXAutomation
transduction in a collagenbased scaffold
term toxicity in liver
microtissues
XXMethods
development for
spheroid formation in hanging
drop plates
XXAutomation
of 3D cell culture
work flows
XXCell
invasion assays using
spheroids formed in ULA
microplates
Phenotypic Assays and Screening
XXHypoxia
assays in keratinocytes
and spheroids
XXOxidative
stress assays for
ROS production
XXCell
cycle using nuclear stains and
sensors
invasion and migration in
FluoroBlok microplates
and analysis of drug
absorption in Caco-2 and
MDCK cells
XXCharacterization
of multi-drug
resistance transporters
Biologics/Biosimilars
XXADCC
assays using nonradiometric detection and freshly
isolated NK cells, cryopreserved
NK cells, an NK cell line and a
bioluminescent cell reporter
XXImmunogenicity
assays with
AlphaLISA beads
XXBridging
assay comparisons
between solution ELISA
and AlphaLISA
BioTek application notes, white papers,
poster presentations, citations and sample files
are available at: www.biotek.com/resources
64
Service & Support n Applications Support
XXCell-based
assays for blocking
antibodies
XXAggregation
assay using a
molecular rotator-type
fluorescent probe
Food Safety and Quality
XXFood
freshness assay based on
ATP depletion
XXDetermination
of E coli and other
pathogens in lettuce wash
XXAntioxidant
potential using an
ORAC Assay
XXSemi-automated
ELISA assay for
melamine in milk
XXAnalysis
of histamine in wine
Biofuel Research
XXDetermination
of algal cell
lipids using Nile Red
XXMonitoring
algal growth using
their intrinsic properties
XXEnzymatic
digestion of
polysaccharides
XXIdentification
of biofuel
producing bacteria through
temperature resistance
XXMonitoring
enzymatic glucose
production from cellulosic
feedstock
Global Service & Support
Extend the life of your BioTek instrument, and protect your research results, with BioTek’s service professionals. Our
service experts in the field and at our regional service centers receive extensive, ongoing training at our headquarters to
stay abreast of the latest products, and service techniques. Our products and services are compliant with FDA, GLP and
ISO requirements. With all of this information at hand, our service experts help you to maintain precise results over the
life of your BioTek instrument while providing an experience that is superior to our competition.
For any service or support need, contact us at TAC@biotek.com or (888) 451-5171.
Field Service
Customer Resource Center (CRC)
Our team is ready to visit your
laboratory and provide:
BioTek’s Customer Resource
Center gives customers access to
information about their specific
BioTek microplate instrumentation
and software. This web site makes
it easy for customers to acquire
relevant and necessary information
about their products.
Customers can:
XXInstallation,
Training and
Installation Qualification
XXOperational
XXPreventive
Qualification
Maintenance
XXInstrument
Upgrades and
Software Upgrades
XXRepairs
XXTrack
orders
XXMaintain
equipment inventory
Regional Service Centers
XXAccess
BioTek Service Centers are located
across the globe ready to service
your BioTek products:
XXDownload
XXTest
XXRequest
Plate Certification
XXPreventive
Maintenance
XXInstrument
Upgrades
warranty information
technical information,
user manuals and software
updates
service and technical
support
XXDispense
Cassette
Refurbishment
Technical Assistance Center (TAC)
BioTek’s TAC is staffed with skilled
scientists and engineers available
to provide technical assistance
for instrumentation, software and
applications.
Access to BioTek’s Customer Resource Center and
more details on BioTek’s service and support are
available at www.biotek.com
Service & Support n Global Service & Support
What customers say:
“The training was excellent and I learned
a lot of very useful and important
information. I’m really impressed with
the amount of time they took to explain
everything to me and the details they
went over. It was a very practical and
user-friendly training.”
“Always excellent experience and
service. It can’t be “better” because it’s
always the best. Thanks.”
www.biotek.com
65
BioTek is a family-run organization launched in 1968. In 1981 BioTek entered the microplate instrument arena
and introduced its first microplate reader. Since then, BioTek has emerged as a global leader in microplate-based
solutions that increase the productivity for customers engaged in healthcare, pharmaceutical, agricultural and research
applications. Today, BioTek is completely focused on microplate instrumentation, automation and software.
1968 - 79
BioTek
develops,
manufactures
and sells
biomedical
testing
equipment
1987
BioTek introduces
the EL320 Stacking
Automated Reader for automatic reading of
up to 25 plates
1980
BioTek’s first
laboratory instrument,
the EL307 EIA is
launched
1969
BioTek moves
to its first facility
located on East
Spring Street,
Winooski, VT
u
1968 - 1979
BioTek moves to
Burlington, VT
1983
BioTek awarded U.S.
patent for EL307’s
microwell position
indicator
u 1982
EL307B microplate
reader introduced by
BioTek
1980 - 1985
1984
BioTek’s first
Automated Microplate
Reader, the EL310,
the EL308 Microplate
Reader and the first
Microplate Washer, the
EL402, are introduced
u 1985
BioTek introduces
the EL309 Microplate
Reader, and the
EL401 Microplate
Strip Washer
BioTek moves to
current location in
Winooski, VT
66
BioTek n Decades of Innovation
u 1988
BioTek introduces
the EL311 and EL312
Microplate Readers,
EL403 Automated
Microplate Washer,
the EL301 Manual
Strip Reader for field
use, and Kineticalc PC
Software
1986 - 1990
1989
EL340 Biokinetics
reader, EL944 Turbo
Software for EL311
and EL312 clinical data
reduction and the
ELP-35 Automated
Strip Washer are
introduced by BioTek
D
e
c
a
d
e
1991
Kineticalc II
software is
introduced by
BioTek
BioTek begins
development of
Omni System
for automated
high throughput
infectious disease
diagnostics and
screening
o
f
i
n
n
o
BioTek introduces
ELx808
u 1992
Ceres 900 launched by
BioTek
1991 - 1995
1993
BioTek launches ELs1000
automated ELISA system
u 1994
BioTek introduces the
FL500 Fluorescence
Microplate Reader and
ELP-40 Microplate Strip
Washer
1998
µQuant and
PowerWaveX
Microplate
Spectrophotometers
and KCJunior
software are
introduced by
BioTek
t
i
u 2000
Precision 2000
Microplate Sample Processor and
Precision Power
Software introduced
by BioTek
o
n
u 2002
BioTek sells Biomedical
division to focus solely
on Laboratory Microplate
business
BioTek opens European
Coordination Center in
Germany
BioTek launches Synergy
HT, PowerWave XS,
BioStack
2001 - 2005
2003
BioTek enters into an
agreement with UVM
and Immucor, Inc to
develop a practical
fluorescence based
platelet assay for
determining platelet
activation
1999
BioTek launches
FLx800, ELx405,
ELx405M
BioTek awarded U.S.
patent for the quartz
BioCell cuvette for
fixed 1 cm vertical
photometry
a
u 1997
BioTek introduces
the PowerWave,
our first Microplate
Spectrophotometer, the
FL600 Multi-Detection
Reader, the ELx50
Microplate Strip Washer
and KC4 Data Reduction Software
1996 - 2000
BioTek awarded
U.S. patent for the
ELx405 “Dual-Action”
manifold
v
2001
Powerwave HT,
MicroFill are
launched by BioTek
1996
BioTek receives
ISO9001 certification
BioTek enters into an
agreement with Immucor,
Inc, to develop the first
automated blood typing
and crossmatching
analyzer, the ABS2000
1995
ELx800 Microplate
Reader, EL404 Microplate
Washer, and KC3 for
Windows data analysis
software are introduced
by BioTek
s
2005
BioTek launches its
customer newsletter,
TekTalk
u 2004
Precision XS, Clarity
introduced by BioTek
BioTek finalizes an
agreement with
Immucor to develop
the next generation
blood typing and
cross matching
instrument, the
Galileo Echo
www.biotek.com
67
2011
BioTek opens
South Korea office
2006
Synergy 2,
NanoQuot, Gen5
software are
launched by BioTek
BioTek introduces ELx405
Select Deep Well, Eon,
Gen5 version 2.0
u 2012
405 Touch, Synergy Neo,
BioStack3, Gas Controller
for Synergy H1, 405 LS are
launched by BioTek
u 2007
BioTek opens China,
India, Singapore
offices
2008
BioTek launches
MicroFlo Select,
EL406
Synergy 4, Liquid
Handling Control
software are
introduced by BioTek
BioTek launches
online Customer
Resource Center
BioTek opens Shanghai office
Web site offers content
in 10 languages
2011 - 2014
2006 - 2010
2013
BioTek introduces MultiFlo FX,
BioStack 4 and 405 Verify
2009
Epoch, Take3, Synergy Mx
are introduced by BioTek
2010
BioTek launches Synergy
H1, Synergy H4, Synergy
2 Alpha, MultiFlo
u 2014
BioTek launches MultiFlo FX RAD,
Epoch 2, Synergy HTX
and Cytation 5
u
BioTek is awarded
Best Place to Work in
Vermont from VT Business
Magazine and Business
of the Year from the Lake
Champlain Regional
Chamber of Commerce
BioTek launches multilingual web site
BioTek receives EP Patent
for Verify technology and
Ultrasonic Advantage
washer technologies
BioTek opens
Taiwan and Japan offices
BioTek enters the
imaging market with the
Cytation5 Cell Imaging
Multi-Mode Reader
BioTek wins Vermont
Deane C. Davis
Outstanding Business
of the Year award
BioTek receives US Patent for
Verify technology and Ultrasonic
Advantage washer technologies
BioTek opens
Switzerland office
www.biotek.com
68
At BioTek, our philosophy transcends conventional thinking and challenges the old ways.
We develop fresh, original solutions by unifying concepts that often appear to be opposed.
It means to shape and reshape. To engineer, build, deliver and support products that best
serve the marketplace by providing what you need, when you need it.
Think Possible. It’s the difference between leading and following.
BioTek Instruments, Inc.
Tel: (802) 655-4040 • Toll-Free: (888) 451-5171 • Outside the USA: (802) 655-4740
www.biotek.com
World Headquarters, United States
BioTek U.S.
Phone: (802) 655-4740
Toll Free: (888) 451-5171
Service Toll Free: (800) 242-4685
Email: CustomerCare@biotek.com
Service Email: TAC@biotek.com
www.biotek.com
China
BioTek China-Beijing
Phone: +86 (10) 85865569
Email: infochina@biotek.com
www.biotekchina.com.cn
BioTek China-Shanghai
Phone: +86 (021) 50435800
Email: infochina@biotek.com
www.biotekchina.com.cn
France
BioTek France
Phone: +33 (3) 89206329
Email: info@biotek.fr
www.biotek.fr
Germany
BioTek Germany
European Coordination Center
Phone: +49 (0) 71369680
Email: info@biotek.de
www.biotek.de
India
BioTek India
Phone: +91 (22) 6687 0046
Email: biotek@biotek.in
www.biotek.in
Japan
BioTek Japan
Phone: +81 (0)3 5812 8109
Email: infojapan@biotek.com
www.biotek.com/ja
Singapore
BioTek Singapore
Phone: +65 65922100
Email: singapore@biotek.com
www.biotek.com
South Korea
BioTek South Korea
Phone: +82 (0)2 5624740
Email: korea@biotek.com
www.biotekinstruments.co.kr
Switzerland
BioTek Switzerland
Phone: +41 (41) 2504060
Email: info@biotek.ch
www.biotek.ch
Taiwan
BioTek Taiwan
Phone: +886 (2) 26277725
Email: infotaiwan@biotek.com
www.biotek.com
United Kingdom
BioTek UK
Email: CustomerCare@biotek.com
www.biotek.co.uk
For the rest of the world,
contact your local distributor
or BioTek U.S. at:
Phone: +1 (802) 655-4740
Email: CustomerCare@biotek.com
AlphaScreen and AlphaLISA are registered trademarks of PerkinElmer, Inc. • DLR and DLReady logo are trademarks of Promega Corporation.
Dual-Luciferase is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark Office.
FLIPR is a registered trademark of Molecular Devices, Inc.
xMAP is a registered trademark of Luminex Corporation.
Excel, Microsoft, Windows, Windows XP are registered trademarks of Microsoft Corporation in the United States and other countries.
All other trademarks are the property of BioTek Instruments, Inc. or their respective owners.
www.biotek.com
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