DryEase® Mini-Gel Drying System

DryEase® Mini-Gel Drying System
USER GUIDE
DryEase® Mini-Gel
Drying System
For even, crack-free drying of mini-gels
Catalog Numbers NI2387, NI2380
Revision Date 23 March 2012
Publication Part Number IM-2380
MAN0000730
For Research Use Only. Not intended for any animal or human
therapeutic or diagnostic use.
ii
Contents
Kit Contents and Storage............................................................................................ iv Introduction .................................................................................. 1 Product Description ..................................................................................................... 1 Methods ........................................................................................ 2 Gel Drying Protocol ..................................................................................................... 2 Troubleshooting ............................................................................................................ 4 Appendix ...................................................................................... 5 Accessory Products ...................................................................................................... 5 Technical Support ......................................................................................................... 6 Purchaser Notification ................................................................................................. 8 iii
Kit Contents and Storage
Types of Kits
This manual is supplied with the following products:
Product
®
Kit Contents
Catalog no.
DryEase Mini-Gel Drying System
NI2387
DryEase® Mini-Gel Drying Frame
NI2380
The DryEase® Mini-Gel Drying Frame contains 4 frames and
8 clamps.
The components of the DryEase® Mini-Gel Drying System
are listed below. Store the Gel-Dry™ Drying Solution at
room temperature. The solution is stable for one year when
stored properly.
Components of the DryEase® Mini-Gel Drying System
Amount
®
2 each
®
1 each
®
200 pre-cut sheets
™
Gel-Dry Drying Solution (1X)
500 mL
Gel-Dryer Clamps
8 each
DryEase Mini-Gel Dryer Frame
DryEase Mini-Gel Dryer Base
DryEase Mini Cellophane
Gel-Dry™
Drying
Solution
The Gel-Dry™ Drying Solution contains a proprietary nonglycerol component to effectively regulate the rate of drying
and prevent cracking. The gel drying solution does not
interfere with autoradiography. Ordering information is
provided on page 5.
Product Use
For research use only. Not intended for human or animal
diagnostic or therapeutic uses.
WEEE (Waste Electrical and Electronic Equipment) symbol
indicates that this product should not be disposed of in
unsorted municipal waste. Follow local municipal waste
ordinances for proper disposal provisions to reduce the
environmental impact of WEEE.
iv
Introduction
Product Description
DryEase®
Mini-Gel
Drying
System
Product
Specifications
The DryEase® Mini-Gel Drying System is a fast and easy
way to consistently dry mini-gels without cracking. Gels dry
by passive evaporation overnight on your bench top,
eliminating the need for expensive heat or vacuum dryers.
Gels dried with the DryEase® Mini-Gel Drying System are
suitable for fluorography, photography, densitometry,
autoradiography, or permanent gel storage.
Inside Frame Dimensions:
Base Dimensions:
Gel Capacity:
Cellophane Dimensions:
Pore Size of Cellophane
10 cm × 10 cm
18 cm × 18 cm × 4.5 cm
1 mini gel
12 cm × 12 cm
40 microns
Materials and
Maintenance
Frame material:
Polycarbonate
Base material:
High Impact Styrene
Chemical resistance
Parts are impervious to alcohol. Do not use with organic
solvents such as acetone or toluene.
Maintenance
To clean the frames, clamps, and base, rinse in deionized
water and a mild detergent.
Clamp Maintenance
To restore clamps to their original shape, dip them in hot
water and bend the clamps back into shape.
Compatibility
with Autoradiography
The Gel-Dry™ Drying Solution used in the DryEase® Mini-Gel
Drying System is compatible with the enhancers used for
autoradiography.
After electrophoresis is complete, incubate the gel in the
desired enhancer (follow manufacturer’s recommendations)
and then wash the gel thoroughly in deionized water. Proceed
with drying the gel as described in the protocol (page 2).
The cellophane does not interfere in detecting the signal
using 32P. Depending on the type of enhancer used for
autoradiography, you may need to remove the upper layer of
the cellophane to detect the signal using 35S.
1
Methods
Gel Drying Protocol
Materials
Needed
•
Deionized water
•
StainEase® Gel Staining Tray (see page 5 for ordering
information) or a round covered container
•
Gel Knife or razor blade
If you have ordered DryEase® Mini-Gel Drying Frame, the
following additional items are also needed:
Procedure
•
DryEase® Mini-Gel Drying Base (see page 5)
•
DryEase® Mini Cellophane (see page 5)
•
Gel-Dry™ Drying Solution (or prepare a solution
containing 30% methanol and 5% glycerol)
1.
2.
3.
4.
5.
After completion of staining and destaining steps,
wash the destained gel(s) three times for two minutes
each time in deionized water (50 mL per mini-gel) on a
rotary shaker.
Decant the water and add fresh Gel-Dry™ Drying
Solution (35 mL per mini-gel).
Equilibrate the gel in the Gel-Dry™ Drying Solution by
shaking the gel for 15–20 minutes in the StainEase® Gel
Staining Tray or in a round covered container.
Note: If drying gels stained with Colloidal Blue Stain
or SimplyBlue™ SafeStain, equilibrate the gel in the
Gel-Dry™ Drying Solution for only 5 minutes. Gels left
in Gel-Dry™ Drying Solution for longer than 5 minutes
lose band intensity and detection limits may decrease.
Cut any rough edges off the gel (including the wells
and the gel foot) using the Gel Knife or a razor blade.
Note: Be sure to push the blade perpendicularly
through the gel to prevent generating any tears on the
gel that can act as a starting point for crack formation
during drying.
Remove 2 sheets (per gel) of cellophane from the
package.
Continued on next page
2
Gel Drying Protocol, continued
Procedure,
continued
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Immerse one sheet of cellophane at a time in the GelDry™ Drying Solution, making sure to cover both sides
of the sheet with solution. Allow 15–20 seconds for
complete wetting before adding additional sheets. Do
not soak the cellophane sheets for more than
2 minutes.
Place one side of the DryEase® Gel Drying Frame with
the corner pin facing up, on the DryEase® Gel Drying
Base.
Center a piece of pre-wetted cellophane from Step 6
over the base/frame combination, so the cellophane
lays over the inner edge of the frame.
Lay the gel on the center of the cellophane sheet
making sure no bubbles are trapped between the gel
and the cellophane. Add some Gel-Dry™ Drying
Solution to the surface of the cellophane, if necessary.
Carefully lay the second sheet of cellophane over the
gel so that no bubbles are trapped between the
cellophane and the gel. Add some Gel-Dry™ Drying
Solution if necessary. Gently smooth out any wrinkles
in the assembly with a gloved hand.
Align the remaining frame so that its corner pins fit
into the appropriate holes on the bottom frame. Push
the plastic clamps onto the four edges of the frames.
Lift the frame assembly from the DryEase® Gel Drying
Base and pour off the excess solution from the base.
Stand the gel dryer assembly upright on a bench top.
Be careful to avoid drafts as they can cause an uneven
rate of dying which leads to cracking. Drying takes
between 12–36 hours depending on humidity and gel
thickness.
Note: Do not leave gels on the frame for longer than
48 hours, as cracks may form in the gel.
When the cellophane is dry to touch, remove the
gel/cellophane sandwich from the drying frame.
Trim off the excess cellophane.
Press the dried gel(s) between the pages of a notebook
under light pressure for approximately 2 days so that
gels remain flat for scanning, photography, display,
and overhead projection.
3
Troubleshooting
Observation
Cause
Solution
Gels falling
apart in
stain/destain
• Shaking motion too
vigorous
• Decrease shaker speed and use
round container for shaking.
• Sharp utensils used
to transfer gel
• Use smooth plastic utensil.
Gels cracking
during drying
• Air trapped between
cellophane layers
• Apply adequate Gel-Dry™ Drying
Solution. Gently smooth out any
bubbles in the assembly with a
gloved hand.
• Strong tension on
cellophane
• Rough edges or
small cracks on gel
• Uneven clamping
pressure
• Gel was not
adequately soaked in
Gel-Dry™ Drying
Solution
Gels turning
white after
drying
4
• Do not stretch cellophane on the
frame.
• Trim rough edges and small
cracks.
• Place clamps evenly around
frame.
• Equilibrate the gel in Gel-Dry™
Drying Solution for 20 minutes.
• Gels drying too fast
• Remove the gel from any drafts.
• Drying in less than
30% humidity
• Rehydrate the gel with deionized
water while the gel is still on the
frame.
Appendix
Accessory Products
The table below lists additional products that may be used
for staining or drying the gel.
Additional
Products
Product
™
SilverQuest Silver Staining Kit
™
Quantity
1 kit
Catalog no.
LC6070
SimplyBlue SafeStain
1L
LC6060
SilverXpress® Silver Staining Kit
1 kit
LC6100
Colloidal Blue Staining Kit
1 kit
LC6025
DryEase® Mini-Gel Drying Frame
2/pk
NI2380
®
1 each
NI2300
®
DryEase Mini Cellophane
200/pk
NC2380
Large Gel Drying Frame
1 each
NI2200
Large Cellophane
100/pk
NC2200
™
Gel-Dry Clamps
1 each
NI2100
™
500 mL
LC4025
DryEase Mini-Gel Drying Base
Gel-Dry Drying Solution (1X)
8 × 500 mL
®
StainEase Staining Tray
2/pack
LC4025-4
NI2400
5
Technical Support
Obtaining
support
For the latest services and support information for all
locations, go to www.lifetechnologies.com/support.
At the website, you can:
• Access worldwide telephone and fax numbers to contact
Technical Support and Sales facilities
• Search through frequently asked questions (FAQs)
• Submit a question directly to Technical Support
(techsupport@lifetech.com)
• Search for user documents, Safety Data Sheets (SDSs),
vector maps and sequences, application notes,
formulations, handbooks, certificates of analysis,
citations, and other product support documents
• Obtain information about customer training
• Download software updates and patches
Safety Data
Sheets (SDS)
Safety Data Sheets (SDSs) are available at
www.lifetechnologies.com/support.
Certificate of
Analysis
The Certificate of Analysis provides detailed quality control
and product qualification information for each product.
Certificates of Analysis are available on our website. Go to
www.lifetechnologies.com/support and search for the
Certificate of Analysis by product lot number, which is
printed on the box.
Continued on next page
6
Technical Support, continued
Limited
Product
Warranty
Life Technologies Corporation and/or its affiliate(s)
warrant their products as set forth in the Life
Technologies’ General Terms and Conditions of Sale
found on Life Technologies’ website at
www.lifetechnologies.com/termsandconditions. If
you have any questions, please contact Life
Technologies at www.lifetechnologies.com/support.
7
Purchaser Notification
Limited Use
Label
License:
Research
Use Only
The purchase of this product conveys to the purchaser the
limited, non-transferable right to use the purchased amount
of the product only to perform internal research for the sole
benefit of the purchaser. No right to resell this product or
any of its components is conveyed expressly, by implication,
or by estoppel. This product is for internal research purposes
only and is not for use in commercial services of any kind,
including, without limitation, reporting the results of
purchaser’s activities for a fee or other form of consideration.
For information on obtaining additional rights, please
contact outlicensing@lifetech.com or Out Licensing, Life
Technologies, 5791 Van Allen Way, Carlsbad, California
92008.
© 2012 Life Technologies Corporation. All rights reserved.
The trademarks mentioned herein are the property of Life
Technologies Corporation or their respective owners.
LIFE TECHNOLOGIES CORPORATION AND/OR ITS
AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH
RESPECT TO THIS DOCUMENT, EXPRESSED OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT
ALLOWED BY LAW, IN NO EVENT SHALL LIFE
TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE,
WHETHER IN CONTRACT, TORT, WARRANTY, OR
UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE,
MULTIPLE OR CONSEQUENTIAL DAMAGES IN
CONNECTION WITH OR ARISING FROM THIS
DOCUMENT, INCLUDING BUT NOT LIMITED TO THE
USE THEREOF.
8
Notes
9
Notes
10
Headquarters
5791 Van Allen Way | Carlsbad, CA 92008 USA
Phone +1 760 603 7200 | Toll Free in USA 800 955 6288
For support visit
www.invitrogen.com/support or email techsupport@invitrogen.com
www.lifetechnologies.com
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