10) Gel Dryer Instruction Manual

10) Gel Dryer Instruction Manual
Instruction Manual
Vacuum Heated Gel Dryers
GDVH
GDVH35
1
Index
1.
Packing List and Introduction
- 3
2.
Safety
- 4
3.
Controls and Feature
- 5
4.
Operating instructions
- 6
5.
Temperature Calibration
- 8
6.
Maintenance and Cleaning
- 8
7.
Troubleshooting
- 9
8.
Additional Information
- 10
9.
Warranty
- 12
2
PACKING LISTS:
Main
Mains
Silicone
Porous
Instruction
Unit
Lead
Rubber
gel
Manual
support
GDVH
GDVH35
The packing lists should be referred to as soon as the units are received to
ensure that all components have been included. The unit should be checked
for damage when received.
Please contact your supplier if there are any problems or missing items.
1. Introduction
These gel dryers contain optimal sealing using a silicon rubber mask. The microprocessor
controlled temperature (from ambient +5 to 90 with timer from 0 to 999 mins) allows
precise control. The gels are heated using a base plate and the vacuum removes the
moisture from below to dry the gel homogeneously. When applying the vacuum a groove
that frames the drying surface provides a completely tight seal during drying. The drying
area for GDVH is 21 x 31 cm and GDVH35 is 35 x 45 cm.
3
2. Safety
Use the same precautions as with any electrical device.
Before connecting this device to the electrical supply, check
that your supply voltage is within the range stated on the rating
label.
This device must be earthed.
Do not attempt to operate the device if damaged.
Place the unit in a safe, dry location.
The unit MUST NOT touch things in the surrounding area
especially not heat sensitive materials.
Protect this unit from physical damage, corrosive agents and
extreme temperatures.
Do not operate in a damp, humid environment where
condensing moisture may short out internal electrical
components.
When moving to or from a cold room, wait at least eight (8)
hours for the unit to adopt ambient temperature and for
condensing moisture to dry.
Do not place any kind of device on top of the Geldryer.
Do follow the safety precautions for chemicals/dangerous
materials!
WARNING: The Geldryer is designed for use with one
voltage supply: 115 Volts, 60 Hz or 230 Volts, 50 Hz.
Before connecting this unit to the electrical supply,
check that your supply voltage and frequency are within
the stated range.
4
3. Controls and Features
Please refer to figure 1 below for the location of the following controls and features.
1. RED LED – Indicated on Setting Value
2. RED LED – Indicated on Heating
3. LED Display – for Temperature
4. UP Key -- to increase temperature value
5. DOWN Key - to decrease temperature value
6. START/STOP Key – activate or stop the unit.
7. LED Display – for Timer
8. DOWN Key - to decrease time value
9. UP Key -- to increase time value
1. on SETTING VALUE
2. on INCREASING TEMPERATURE
7. TIME Display
3. TEMPERATURE Display
Heating
Set Temp
TEMPERATURE
TIME
START
STOP
8. DOWN Key
9. UP Key
4. UP Key
5. DOWN Key
6. START/STOP Key
figure 1
5
4. Operating instructions
Attention: Always wear gloves when preparing or handling polyacrylamide gels,
because acrylamide is neurotoxic.
1. Place the Gel Dryer on a sturdy, level surface in a safe, dry place away from laboratory
traffic.
2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord into a
grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V as indicated
on the rating sticker near the AC cord on the back of the unit ).
3. Most polyacrylamide gels are dried at 80°C. Highly cross – linked and very thick gels or
gradient gels should be dried at temperatures about 50 to 60°C only.
4. The drying time of a gel depends on size and thickness of the gel and on the capacity of
the membrane vacuum pump used. In most cases the drying procedure needs 2 hours.
We recommend using the Gelmaster Pump – PUMP as this provides even gel drying
with no cracking.
5. Place two layers of filter paper onto the porous gel support.
6. If you wish to evaluate the gel with a densitometer, we recommend placing saran wrap
underneath the gel.
7. Place the gel on the filter paper/saran wrap and remove air bubbles.
8. Cover the gel with a slightly larger piece of porous saran wrap.
9. Bring the rubber sealing plate in position and supply a sufficient vacuum.
10.
Turn the AC power ON, which is located on the front of the unit.
11.
Choose the temperature according to the gel to press the Temperature UP or DOWN
key to adjust desired temperature.
12.
If you want to set time, you can press TIMER Key UP or DOWN to adjust the time to
the appropriate level. The timer will count down when the unit is started. If the timer
isn’t set then the time will count up.
13.
Press the START Key to start heating.
6
14.
When completed, press START Key again to stop the unit. If the timer is set then
the unit will stop heating once the set time is complete.
15.
An indication that the drying procedure is completed is when the surface of the
rubber sealing plate becomes warm above the gel.
16.
Keep the gel under vacuum for some more time after stopping the gel dryer because
opening the system suddenly with the gel still at a high temperature still might cause
the gel to tear.
7
5. Temperature Calibration
1. Place a 45 mm calibrated laboratory Thermometer onto the centre of the Gel Dryer
surface.
2. Switch the main power OFF/ON and press the START key simultaneously. You will
hear a “DU~DU” sound from the dryer and you will find the LED segment will be
flashing (left down segment). The dryer is now in the Calibration Mode.
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3. Press the UP or DOWN key to adjust the display value to the value on the thermometer.
Then press the START key.
4. Wait for around 40~50 min until “all LED” is flashing.
5. Adjust the display value to the same as the thermometer. And then press the START
key again.
6. The calibration procedure is now completed. Wait for some minutes so that the
microprocessor can auto adjust the temperature until the LED display value is the
same as on the thermometer.
6. Maintenance and Cleaning
The Gel Dryer may be cleaned with a moist cloth containing a mild soap solution. The
blocks are aluminum and may be cleaned with any of the commercial aluminum cleaners
on the market taking care to thoroughly rinse any residual cleaning residue before use.
8
7. Troubleshooting
Problem
Comment
LED does not light up
1. Check the FUSE to see if it is
broken
2. Ensure that the AC power
switch is ON.
3. Check the three-pronged power
cord are properly plugged into a
grounded three-prong AC outlet
of the appropriate voltage.
Timer is running, thermostat is
1. Check the temperature setting.
heating, but the gel does not dry
2. Check the vacuum: position of
or takes extremely long time to dry
the silicone, tubing defects,
capacity of the vacuum pump
Gel is torn during drying
1. Drying time is too short.
2. Temperature is too high.
3. Vacuum is not sufficient
Gel is distorted during drying
1. Temperature too high for thick
or highly cross-linked or
gradient gels.
2. The vacuum was switched off to
early and the gel was still
warm.
9
8. Additional Information
Specification and ordering information
Drying Area
GDVH
GDVH$
GDVH35
GDVH35$
21 x 31 cm
21 x 31 cm
35 x 45 cm
35 x 45 cm
Replacement
Silicon
Rubber
S2131
S3545
M2131
M3545
Order
Number
Replacement
Supporting
Mask order
number
Controller
Display
Digitals microprocessor controller
Two of 4 digital LED
Operating
Temperature
Ambient + 5 to 90
Range
Temp.
Increment
0.1 Degrees Centigrade
Temp.
uniformity
+/- 0.2 Degrees Centigrade
Temp.
Calibration
Timer
Yes
0 ~ 999 mins
10
Warranty
The omniPAGE Vertical Electrophoresis units have a warranty against manufacturing
and material faults of twelve months from date of customer receipt.
If any defects occur during this warranty period, your supplier will repair or replace the
defective parts free of charge.
This warranty does not cover defects occurring by accident or misuse or defects caused
by improper operation.
Units where repair or modification has been performed by anyone other than your
supplier or an appointed distributor or representative are no longer under warranty from
the time the unit was modified.
Units which have accessories or repaired parts not supplied by your supplier or it’s
associated distributors have invalidated warranty.
Your supplier cannot repair or replace free of charge units where improper solutions or
chemicals have been used. For a list of these please see the Care and Maintenance
subsection.
If a problem does occur then please contact your supplier.
11
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