FlashGel Power Supply

FlashGel Power Supply
FlashGel™ Power Supply
Instruction Manual
Lonza Rockland, Inc.
www.lonza.com
scientific.support@lonza.com
Scientific Support: 800-521-0390
Customer Service: 800-638-8174
Rockland, ME 04841
Table of Contents
Safety Information
2
Maintenance
3
Specifications
4
Introduction
7
Operation Instructions
9
Troubleshooting
12
Ordering Information
15
Warranty and Liability Information
17
1
Flas
shGel™ Power Supply
Imp
portant Safety
S
In
nformatiion
Saffety sym
mbols
The following symbols alert the user
u
to im
mportant operationa
al, mainten
nance,
nty require
ements, or
o possible
e hazard e
exposure.
and//or warran
Symbol
S
ind
dicates a wa
arning of po
otential exp
posure to ha
azardous vo
oltage
where
w
conttact may result in deatth or serious injury.
Symbol
S
ind
dicates disp
posal instrucction. DO N
NOT throw tthis unit into
oa
municipal trash bin. Fo
or utmost p
protection o
of the global environme
ent and
to
t minimize
e pollution, please recyycle this un
nit.
neral Prod
duct Warrning Stattement:
Gen
UTION: Hazardous
s Voltage
e
CAU
Con
ntact may
y cause death
d
or serious
s
in
njury
Cau
ution should be exerrcised in the
t operattion of thiss system a
as it can d
develop
suffiicient volta
age and current
c
to produce a lethal sh
hock. To
o reduce rrisk of
injurry, the sys
stem shou
uld only be
e operated
d by prope
erly traine
ed personn
nel and
alwa
ays in acc
cordance with
w the in
nstructions provide
ed.
ng on the DC power source, ensure th
hat the bla
ack lead iss
Prior to turnin
conn
nected to the negative terminal and th
he red lea
ad is connected to tthe
posiitive terminal. Failure to adhe
ere to the
ese instrucctions cou
uld result iin
pers
sonal and /or labora
atory haza
ards, as w
well as invvalidate an
ny warran
nty.
Alwa
ays turn off
o the DC power so
ource prio
or to removing casssettes from
m the
dock
k. For maximum safety,
s
alw
ways operrate this ssystem in an isolate
ed, low
trafffic area no
ot accessiible to una
authorized
d personn
nel. Neverr operate
dam
maged or broken
b
eq
quipment.
2
Use a high level of precaution with any electrical device. Before connecting
the electrical supply, check to see if the supply voltage is within the range
stated on the rating label, and see to it that the device plug be firmly seated.
Place the unit in a safe and dry location; it must NOT touch any surrounding
objects. Follow the safety precautions for chemicals / dangerous materials.
If needed, please contact a qualified service representative or
scientific.support@lonza.com.
Environmental Conditions
Ensure the instrument is installed and operated strictly under the following
conditions:







75 kPa – 106 kPa
Pollution degree: 2
Main supply voltage fluctuations up to ±10% of the normal voltage
Temperature: 15C-40C
Humidity: ≤95% relative humidity, non-condensing
For indoor use only
Altitude up to 2000m
Maintenance
Visually inspect the power supply prior to use for signs of wear, cracks or
damage. Do not use if damage is found.
There are no user serviceable parts contained in the FlashGel™ Power
Supply.
3
Specifications
Electrical Specifications
Output Voltage / Inc.: 10 - 300V / 1VDC
Output Current / Inc.: 10 - 400mA / 1mA
Max. Watt:
60W
Rated Voltage:
100 - 240 V, 50 - 60 Hz, 2A
Output Type:
Constant Voltage or Constant Current
Control:
Microprocessor controller
Timer:
1 - 999 minutes with alarm, continuous
Safety Device:
No load detection; shrouded plugs and sockets
Physical Specifications
Terminal Pairs:
2 Pairs
Display:
3 digit LED
Construction
Polycarbonate housing & aluminum bottom plates
material:
Unit Dimension:
140 x 191 x 84mm
Weight:
~1 kg
Catalog Number
57068 - FlashGel™ Power Supply
ON/OFF
Switch
Main Power Jack
and
Fuse Holder
Fan
Front view
Rear view
4
Warning
The FlashGel™ Power Supply has been tested and found to comply with the limits of CE
regulation. The power supply is marked with ETL which indicates it conforms to US and
Canadian product safety standards. The device also complies with RoHS, indicating it’s
free of certain hazardous substances associated with electrical and electronic equipment
which meets the environmental directive. These limits are designed to provide
reasonable protection against harmful interference when the instrument series is
operated in a commercial environment. This instrument used together with the
FlashGel™ Dock or other electrophoresis chamber, uses, and can radiate, radio
frequency energy, and if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of this instrument in
a residential area is likely to cause harmful interference in which case the user will be
required to correct the interference at their expense. Changes or modifications not
expressly approved by the party responsible for compliance could void the user’s
authority to operate the equipment. It is strongly recommended for the user to read the
following points carefully before operating this equipment.
1.
2.
Read and follow the manual instructions carefully.
Do not alter the equipment. Failure to follow these directions could result in personal
and/or laboratory hazards, as well as invalidate any warranty.
3. Use a properly grounded electrical outlet with correct voltage and current handling
capacity.
4. Disconnect from power supply before maintenance and servicing. Refer servicing to
qualified personnel.
5. Do not use the unit if there is any sign of damage. Replace damaged parts.
6. Do not use in the presence of flammable or combustible material; fire or explosion
may result. This device contains components which may ignite such materials.
7. Refer maintenance and servicing to qualified personnel.
8. Ensure that the system is connected to electrical service according to local and
national electrical codes. Failure to make a proper connection may create fire or
shock hazard.
9. Use appropriate materials and operate correctly to avoid possible hazards of
explosion, implosion or release of toxic or flammable gases arising from overheated
materials.
10. The unit shall be operated only by qualified personnel.
5
Avoiding Electrical Shock
Follow the guidelines below for safe operation of the unit.
The FlashGel™ Power Supply has been designed to utilize shielded wires thus
minimizing potential shock hazard to the user. Lonza recommends against the use of
unshielded wires.
To reduce risk of electrical shock:
1. In the event of solution spilling on the instrument, it must be dried out for at least 2
hours and restored to NORMAL CONDITION before each operation.
2. Never connect or disconnect wires loading from the power jacks when the red
indicator light of power switch is on.
3. WAIT at least 5 seconds after stopping a run before handling output leads or any
connected apparatus.
4. ALWAYS make sure that your hands, work area, and instruments are clean and dry
before making any connections or operating the power supply.
5. ONLY connect the power cord to a properly grounded AC outlet.
Avoiding Damage to the Instrument
1. Do not attempt to operate the device if damage is suspected.
2. Protect this unit from physical damage, corrosive agents and extreme temperatures
(direct sunlight, etc.).
3. For proper ventilation and safety concerns, keep at least 10 cm of space behind the
instrument, and at least 5 cm of space on each side.
4. Do not operate the unit out of environmental conditions previously listed.
5. Prior to applying any cleaning or decontamination methods other than in accordance
with manufacturer’s recommendation, users should check with the manufacturer’s
instructions to see if the proposed method will damage the equipment.
Equipment Operation
Follow the guidelines below for safe operation of the unit:
1. NEVER access dangerous chemicals or other materials to reduce the likelihood of
possible hazard of explosion and damage.
2. Do not operate the unit without lids or covers.
3. A temporary conductivity caused by condensation might occur even though this
series is rated Pollution Degree 2 in accordance with IEC 664.
6
Introduction
The Lonza FlashGel™ Power Supply completes the FlashGel™ System. The
FlashGel™ Power Supply is designed for use with the FlashGel™ Dock and extends its
capabilities outside the FlashGel™ System to most general electrophoresis applications.
The FlashGel™ Device Pack which includes the FlashGel™ Power Supply, FlashGel™
Dock, and FlashGel™ Camera offers an all in one comprehensive package for fast easy
nucleic acid separation. Its compact size, with two terminal pairs which provide sufficient
and accurate output voltages, can deliver reliable experimental results from one
experiment to another. Lonza’s FlashGel™ Power Supply is ETL (US and Canada),
RoHS and CE compliant to address environmental and safety concerns.
FlashGel™ Device Pack
FlashGel™ Camera FlashGel™ Power Supply FlashGel™ Dock For additional information on the FlashGel™ System visit: www.flashgel.com
7
Product Description & Feature
The FlashGel™ Power Supply is powerful enough to provide the constant 225V or 275V
output, which is recommended to run the FlashGel™ Cassettes while being a fraction of
the size of power supplies with similar outputs. Continuous or timed operations are easily
performed using the simple and user-friendly interface. The FlashGel™ Power Supply
features 2 electrode pairs, allowing for up to 2 FlashGel™ Cassettes to be run
simultaneously, saving both time and valuable bench space. With a universal voltage
rating, the FlashGel™ Power Supply is also designed and constructed to rigorous safety
standards.
- Compact size
- Power capacity: 60W, 400mA, 300V
- Constant Voltage or Constant Current operation
- 1 V step voltage selection; 1 mA step current selection
- Timer
- No load detection
- Shrouded plugs and sockets
- Two pairs of outlet terminals
- Output voltage stability
- CE marked, ETL (conforms to US and Canadian product safety standards)
8
Operation Instructions
Control interface
You will find six buttons and four LED indicators on the faceplate.
The LED indicates the status of the unit.
a. Setup Mode (before pressing RUN/Start)
LED light indicates your current parameter. If you are setting a value for Voltage, the
Voltage LED will be lit.
b. Operation Mode
After you start the experiment, the LED light next to the RUN/STOP button will light
up to indicate the unit is under operation.
1.
– Select voltage value
2.
– Select current value
3.
– Timer button
4.
– Increase the Voltage, Current or Time
5.
– Decrease the Voltage, Current, or Time
6.
– Start or stop the unit
9
Start the operation
NOTE: To operate under constant voltage or constant current modes, adjust the other
parameter to the maximum value. For example, to operate under constant voltage,
adjust current to max before running using constant voltage, and vice versa.
1. Place the unit on a sturdy and level surface in a safe, dry place, away from laboratory
traffic.
2. Ensure that the AC power switch is OFF, and then plug the three-pronged power cord
into a grounded three-prong AC outlet with appropriate voltage (100V to 240V as
indicated on the rating sticker near the AC cord on the back of the unit).
3. Power on the unit by pressing the ON/OFF switch.
4. Connect the DC output jacks from the FlashGel™ Dock or other electrophoresis unit;
insert the red lead (+) into the red output jack, and the black lead (-) into the black
output jack.
5. Constant voltage or current
*To set constant voltage, press
, the LED indicator will light up.
*To set constant current, press
, and LED indicator will should light up.
6. Adjust the output value by pressing the
or
button.
*When running FlashGel™ Cassettes, refer to the FlashGel™ Dock instruction
manual for recommended electrophoresis conditions.
7. After adjusting your constant mode output value, switch to the other parameter and
set it to maximum.
8. Timer Setting: After setting the current or voltage output value, press the Timer button,
and then press
button or
button to adjust timer accordingly.
10
Note: When timer is set as “0”, it means continuous operation.
9. Press
next to the
10. Press
button to start the run. Once the electrophoresis starts, the LED light
button will light up.
button again to stop the unit at any time if necessary.
11. When the run is completed, Press
button to terminate a timed run, and turn
the AC power OFF by the switch on the rear.
12. The programmed settings will be automatically saved into the system. The next time
you turn it on, the same settings as your previous experiment will be displayed.
11
Troubleshooting
Many operating problems may be solved by carefully reading and following the
instructions in this manual. Some suggestions for troubleshooting are given below.
Should these suggestions not resolve the problem or if you have any questions, contact
Lonza Scientific Support or the Lonza distributor in your region for assistance. If
troubleshooting service is required, please include a full description of the problem.
Problem
Possible Cause
No AC power
No Display / lights
Repeated fuse
broken
Operation stops
Potential Solution
Check if the power supply is
unplugged, or AC power source
problem
AC power cord is not Check AC power cord connections at
connected
both ends. Use the correct cords.
The fuse has blown
Replace the fuse
Hardware failure
Contact Lonza Scientific Support
Electrophoresis
leads are not
connected to the
power supply or to
the electrophoresis
unit(s), or there is a
broken circuit in the
electrophoresis cell
Check the connections to the power
supply and on your electrophoresis
cell to make sure the connection is
intact; check condition of wires in
electrophoresis unit. Close the circuit
by reconnecting the cables. Press
High resistance due
to tape left on a
pre-cast gel,
incorrect buffer
concentration, or
incorrect buffer
volumes in the
electrophoresis cell*
Correct the condition by making sure
the tape is removed from the precast
gel, buffers are prepared correctly,
and the recommended volume of
buffer is added to the electrophoresis
unit.
START/STOP to restart the run.
12
Er1
Error message
Over current(400mA Check if the buffer concentration is
limitation reached)
appropriate. Excessive buffer
concentration may cause over current
issue.* To clear the error message,
press the START/STOP button again
Er2
Error message
Over voltage(300V
limitation reached)
Press START/STOP button to clear
the error message. Contact Lonza
Scientific Support if the problem
persists.
Er3
Error message
Thermal limitation
reached(Output
voltage <10V)
(1) Check the connections
nLD
Message
No load is detected
(1) Check the connections
(2) Check the buffer condition / buffer
level.
AL1
Alarm message
Max. watts(60W) of
power reached
Warning message for reference
(2) If Er3 error message persists, the
problem may be caused by internal
fan failure. Contact Lonza Scientific
Support.
*Not likely when used with FlashGel™ Dock
Encountering Problems
1. Check the troubleshooting section.
2. Call Scientific Support or e-mail scientific.support@lonza.com
13
Replacing the Fuse
For additional fuses, call Lonza Scientific Support or e-mail
scientific.support@lonza.com
To replace the fuse:
1. Turn off the main power switch at the rear of Power Supply and detach the power
cord.
2. Open the fuse compartment located inside the Power Entry Module by inserting a
small flat blade screwdriver into the slot below the ON/OFF switch. Turn the
screwdriver to gently pry open the fuse compartment.
NOTE: The fuse compartment will not open with the power cord in place.
3. Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned
or there is a break in the fuse element, replace the fuse with the identical type of fuse
(T2AH250V) as provided in the fuse holder (see figure below).
4. Place the fuse holder back into the compartment.
5. Snap the cover closed.
14
Ordering Information
FlashGel™ Devices
Cat No. Description
57068
FlashGel™ Power Supply
57025
FlashGel™ Dock
For use with all FlashGel™ Cassette types
57040
FlashGel™ Camera
Includes Camera, hood enclosure USB cable, and FlashGel™ Capture
Software. For use with the FlashGel™ Dock.
FlashGel™ Device Packs
Cat No. Description
57062
FlashGel™ Device Pack
Includes FlashGel™ Dock, FlashGel™ Power Supply, and FlashGel™ Camera
57069
FlashGel™ Power Supply Pack
Includes FlashGel™ Dock and FlashGel™ Power Supply
57065
FlashGel™ Camera Pack
Includes FlashGel™ Dock and FlashGel™ Camera
FlashGel™ Consumable Kits Cat No. Description
57063
FlashGel™ DNA Kit
Includes FlashGel™ DNA Cassettes 1.2% 12+1 well single tier 9pk,
FlashGel™ Loading Dye, and FlashGel™ Marker 100 bp – 4 kb
57064
FlashGel™ Recovery Kit
Includes FlashGel™ Recovery Cassettes 1.2% 8+1 well double tier 9pk,
FlashGel™ Recovery Buffer, FlashGel™ Loading Dye,
FlashGel™ QuantLadder, and Visualization Glasses
57024
FlashGel™ RNA Kit
Includes FlashGel™ RNA Cassettes 1.2% 12+1 well single tier 9pk,
RNA Marker, Sample Buffer, and Molecular Biology Water
15
Notes
16
Warranty and Limitation of Liability
EXCEPT AS SET FORTH BELOW, LONZA ROCKLAND, INC. MAKES NO WARRANTY OF
ANY KIND, EITHER EXPRESS OR IMPLIED, BY FACT OR LAW, OTHER THAN LONZA'S (I)
OBLIGATION TO DELIVER PRODUCT COMPLYING WITH LONZA'S PUBLISHED
SPECIFICATIONS (OR AS OTHERWISE REFERENCED IN THE CONTRACT) AND (II)
IMPLIED WARRANTIES OF TITLE, FREEDOM FROM ENCUMBRANCE, AND RIGHT TO
TRANSFER SAME. LONZA MAKES NO WARRANTY OF FITNESS FOR A
PARTICULAR PURPOSE, OR WARRANTY OF MERCHANTABILITY OTHER THAN AS
STATED HEREIN.
Lonza Rockland, Inc. warrants this product to be free from defects in materials and workmanship
under normal service for one year from date of shipment, subject to the exclusions set forth
below and provided that the product has been operated and maintained according to the
instructions provided. If the product proves defective during this period, Lonza Rockland, Inc.,
will repair or replace it at our option, free of charge, if returned to us postage prepaid. This
warranty does not cover: Consumable parts (fuse), damage in transit, damage caused by
carelessness, misuse or neglect, normal wear through frequent use, damage caused by solvent
corrosion, damage caused by improper handling or user alteration, nor unsatisfactory
performance as a result of conditions beyond Lonza’s control.
LONZA ROCKLAND INC.'S TOTAL LIABILITY AND BUYER'S EXCLUSIVE REMEDY
FOR ANY CAUSE OF ACTION ASSOCIATED WITH THIS PRODUCT, WHETHER BASED IN
TORT, CONTRACT, STRICT LIABILITY OR ANY OTHER LEGAL THEORY IS
EXPRESSLY LIMITED TO REPLACEMENT OF NONCONFORMING PRODUCT OR
PAYMENT IN AN AMOUNT NOT TO EXCEED THE PURCHASE PRICE OF THE SPECIFIC
PRODUCT FOR WHICH DAMAGES ARE CLAIMED, AT LONZA'S OPTION. LONZA
ROCKLAND, INC., SHALL IN NO EVENT BE LIABLE FOR ANY OTHER DAMAGES
INCLUDING, WITHOUT LIMITATION, INCIDENTAL, SPECIAL, PUNITIVE, OR
CONSEQUENTIAL DAMAGES, INCLUDING WITHOUT LIMITATION, LOST PROFITS, LOSS
OF INCOME, LOSS OF BUSINESS OPPORTUNITIES, LOSS OF USE AND OTHER
RELATED DAMAGES, HOWEVER CAUSED, NOR ANY DAMAGE ARISING FROM THE
INCORRECT USE OF THE PRODUCT.
License and Trademark Information
Some components and technology of the FlashGel™ System are sold under licensing
agreements. The nucleic acid stain in this product is manufactured and sold under license from
Molecular Probes, Inc. and the FlashGel™ Cassette is sold under license from Invitrogen IP
Holdings, Inc. and is for use only in research applications or quality control, and is covered by
pending and issued patents. Dark Reader is a trademark of Clare Chemical Research, Inc. The
FlashGel™ Dock technology contains Clare Chemical Research, Inc. Dark Reader®
transilluminator technology and is covered under US Patents 6,198,107; 6,512,236; and
6,914,250. The electrophoresis technology is licensed from Temple University and is covered
under US Patent 6,905,585.
All trademarks herein are marks of the Lonza Group or its affiliates.
For Research Use Only. Not for use in diagnostic procedures.
Manufactured for Lonza
© 2012 Lonza Rockland, Inc.
17
Lonza Rockland, Inc.
www.lonza.com
scientific.support@lonza.com
Scientific Support: 800-521-0390
Customer Service: 800-638-8174
Document # 00525706-0812-2
Rockland, ME 04841
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