Operation Manual

Operation Manual
ISO 13485
Bottle Top Dispenser
Re-Circulation Valve
Page No.
Intended Use Of The Instrument
Safety Instruction
Functions and Limitations of Use
Operating Exclusions
Storage Conditions
List of Recommended Reagents
First Steps
Error Limits (Nominal Volume, Partial Volume)
User Calibration Procedure
Maintenance / Cleaning
13). Always check the instrument for visual damage before use.
Intended Use Of The Instrument
The Bottle Top Dispenser is a general purpose laboratory instrument intended for use in
laboratories for dispensing reagents and chemicals which are compatible with the
instrument. (see page 4)
14). If there is a sign of a potential malfunction (e.g. piston difficult to move, sticking valve or
leakage). immediately stop dispensing. Consult the 'Troubleshooting' section of this manual
and contact the manufacturer if needed. (see page 20)
Safety Instructions
Functions and Limitations of Use
This instrument may sometimes be used with hazardous materials, operations and equipments.
The bottle top dispenser is designed for dispensing liquids directly from the reservoir bottle.
It is beyond the scope of this manual to address all of the potential risks associated with its use
The instrument is calibrated according to the requirements of the DIN EN ISO 8655 – 5.
in such applications. It is the responsibility of the user of this instrument to consult and establish
When the instrument is correctly used, the dispensed liquid comes into contact with only the
appropriate safety and health practice and determine the applicability of regulatory limitations
following chemically resistant materials:
prior to use.
PTFE, FEP and Borosilicate glass.
Please read the following carefully!
1). Every user must read and understand this operating manual before operation.
2). Follow general instructions for hazard prevention and safety instructions e.g. wear protective
clothing, eye protection and gloves.
Limitations of use :
This instrument is designed for dispensing liquids, observing the following physical limits:
Use temperature from +15°C to +40°C (from 59°F to 104°F) of instrument and reagent
Vapor pressure up to max. 600 mbar. Aspirate slowly above 300 mbar, in order to prevent
3). Observe all specifications provided by reagent manufacturers.
4). When dispensing inflammable media, make sure to avoid the built up of static charge,
e.g. do not dispense into plastic vessels do not wipe instruments with a dry cloth.
5). Use the instrument only for dispensing liquids, with strict regard to the defined limitations of
the liquid from boiling.
Kinematic viscosity 500 mm2
(dynamic viscosity [mPas] = kinematic viscosity [mm2 /s] x density [g/cm³])
Density: up to 2.2 g/cm³
use and operating limitations. (see page 2) Observe operating exclusions.
If in doubt, contact the manufacturer or supplier.
6). Always use the instrument in such a way that neither the user nor any other person is
Operating Limitations :
endangered. When dispensing, the discharge tube must always point away from you or
Liquids, which form deposits may make the piston difficult to move or may cause jamming (e.g.,
any other person. Avoid splashes. Only dispense into suitable vessels.
crystallizing solutions or concentrated alkaline solutions). If the piston becomes difficult to move,
7). Never press down the piston when the discharge tube closure is attached.
the instrument should be cleaned immediately. (see page 15)
8). Never remove the discharge tube while the dispensing cylinder is filled.
When dispensing inflammable media, make sure to avoid buildup of static charge, e.g.
9). Reagents can accumulate in the cap of the discharge tube. Thus, it should be cleaned
do not dispense into plastic vessels, do not wipe instrument with a dry cloth.
The Dispenser is designed for general laboratory applications and complies with the relevant
10). Never carry the mounted instrument by the cylinder sleeve or the valve block. Breakage or
loosening of the cylinder may also lead to personal injury from chemicals.
11). Never use force on the instrument. Use smooth gentle movements to operate the piston
upwards and downwards. Use only original manufacturer's accessories and spare parts.
standards, e.g. DIN EN ISO 8655. Compatibility of the instrument for a specific application
(e.g. trace material analysis, food sector etc.) must be checked by the user. Approvals for
specific applications, e.g. for production and administration of food, pharmaceuticals and
cosmetics are not available.
12). Do not attempt to make any technical alterations. Do not dismantle the instrument any
further than is described in the operating manual.
List of Reagents
Operating Exclusions
Never use with:
Liquids attacking FEP, PFA and PTFE (e.g. dissolved sodium azide*)
Liquids attacking borosilicate glass (e.g. hydrofluoric acid)
Hydrochloric acid > 40% and nitric acid >70% | Tetrahydrofuran | Trifluoroacetic acid
Explosive liquids (e.g. carbon disulfide)
Suspensions (e.g. of charcoal) as solid particles may clog or damage the instrument
Liquids attacking PP (cap)**
* Dissolved sodium azide permitted up to a concentration of max. 0.1%.
** Liquids attacking PP (cap)
Storage Conditions
Store the instrument and accessories only in clean conditions in a cool and dry place. Storage
temperature: from – 20°C to +50°C (from – 4°F to 122°F)
Chemical Resistance
Chemicals from A to Z
The following list includes most frequently used chemicals. It provides useful information for the
safe and adequate use of Beatus. However, safety precautions and
recommendations in operating instructions must be followed carefully.
Code explanations
A = Good resistance
B = Acceptable with limitations
C = Not recommended
1 = Possible crystallisation - blockage or possible coating peeling
(do not let dry plunger/barrel together).
2 = Swell of plunger protection layer, possible peeling.
3 = Acid vapours (better resistance with lower concentration).
Do not leave instrument on bottle.
4 = Risk of damage, softening or discoloration of external parts through vapours.
Do not leave instrument on bottle.
5 = Chemical degradation of glass parts (plunger/barrel).
List of Reagents
List of Reagents
List of Reagents
List of Reagents
First Steps
Is everything in the package ? Confirm that package includes :
Bottle Top Dispenser, discharge tube, telescoping filling tube, calibration tool, different bottle
adapters, a calibration certificate and this operation manual.
All dispensers will have the following adapters : 28, 32, 36, 40 & 45 mm.
Wear protective clothing, eye protection and gloves. Follow all Safety instruction and observe
limitations of use and operating limitations. (see page 2)
1. Adjust length of telescoping inlet tube.
Main Housing
The length of FEP inlet tubing provided should be adjusted
to fit your particular reservoir. Longer length of inlet tube are
Volume Adjustment
(Fig. 1)
available on request. (Fig. 1)
2. Fix the telescoping tube. (Fig. 2)
(Fig. 2)
3. Choose the correct adapter for the bottle.
Chuck Nut
Re-Circulation Valve
Middle Body
Delivery Pipe Cover
The threaded platform base of dispenser has a 30 mm screw
thread. Five adapters are supplied to suit containers with a
28, 32, 36, 40, 45 mm and 30 mm (inbuilt adapter) screw
neck. (Fig. 3)
(Fig. 3)
4. Fix the adapter. (Fig. 4)
Threaded platform
Nozzle Cap
(Fig. 4)
5. Mount the dispenser :
The assembled dispenser is screwed to the reservoir using
gentle hand torque applied to the threaded platform base
only. Removal should also be by means of hand torque
(Fig. 5)
applied to the same base. (Fig. 5)
6. Ready to Use.
Do not operate the piston until the unit is safely and fully mounted on the reservoir bottle.
Always wear protective gloves when touching the instrument or the bottle, especially when
using dangerous liquids. When mounted to a reagent bottle, always carry the instrument as
shown in the figure (5).
Never press down the piston when the cap is on. Avoid splashing the reagent.
1. Volume Setting :
Volume Adjustment Knob :
It is simple and easy to operate. There are two
The reagent can drip out from the discharge tube and cap.
positions of the knob as shown in Fig. 10-A
(Fig. 6)
Position 1 : Locked Position | Position 2 : Unlocked Position
(Fig. 10)
Setting the Volume :
Open the cap of the dispensing tube (Fig. 6). For safety hold the discharge tube orifice on the
Follow these simple steps :
Turn the Knob from Position 1 to Position 2 by rotating it
inner wall of a suitable receiving vessel.
Position 1 Position 2
ANTICLOCKWISE as shown in Fig. 10-B1.
1. Set valve to 'Recirculate' (Fig. 7)
(Fig. 6)
The slider is now loose and can be moved up and down.
Lock to Unlock Position
(Anti Clockwise)
For priming gently pull up the piston approx, 30mm and
Set your desired volume by aligning the pointer with the scale.
push it down rapidly until the lower stop.
To lock the set volume, turn the Knob from Position 2 to Position 1 by
Repeat this procedure 5 times (Fig. 8).
rotating it CLOCKWISE as shown in Fig. 10-B2 .
Unlock to Lock Position
2. Dispensing :
2. Turn valve to 'Dispense' (Fig.9).
Wear protective clothing, eye protection and gloves. Liquid may accumulate in the cap.
To avoid splashes when priming hold the discharge tube on
the inner wall of a suitable receiving vessel and dispense
To avoid splashes dispense slowly. Follow all safety instructions and observe limitations of use
liquid to prime the discharge tube until it is bubble free,
and operating limitations.
(Fig. 7)
Wipe away any remaining drops from the discharge tube.
Remove cap from the discharge tube. (Fig. 11)
(Fig. 11)
Note :
Before using the instrument for the first time, ensure it is
(Fig. 8)
rinsed carefully and discard the first few samples dispensed.
Avoid splashing.
When using the instrument (with recirculation valve) turn the
valve to Dispensing. (Fig. 12)
(Fig. 12)
(Fig. 9)
Hold the discharge tube orifice on the inner wall of a suitable
User Calibration Procedure
receiving vessel. (Fig. 13)
Dispenser has been laboratory calibrated at its nominal volume. However, due to changes in
environmental conditions and the viscosity of the media which you dispense, we recommend
gravimetric testing every 3-12 months. Gravimetric volume testing according to DIN EN ISO 8655-6
(Fig. 13)
(for measurement conditions, see 'Error Limits', page 13) is performed as follows:
Gently lift the piston until the upper stop and then
(Fig. 14)
depress piston slowly and steadily with minimal force
Re-Calibrate :
until the lower stop. (Fig. 14)
1. Set the Dispenser to the nominal volume or any other
volume which is most commonly used by you. (Fig. 16)
Follow the common rules for calibration used in statistical
Wipe off the discharge tube against the inner wall of the
(Fig. 15)
receiving vessel.
quality control (ISO 8655/2). Set the volume and
dispense five full volumes of distilled water at 20°C on
Electronic Balance to establish the actual mean volume of
liquid dispensed. If the gravitational average result varies
Reattach cap to discharge tube. (Fig. 15)
(Fig. 16)
from the volume displayed, you should re-calibrate the
Error Limits
Error Limits related to the nominal capacity (= maximum volume) indicated on the instrument,
are obtained when instrument and distilled water are equilibrated at ambient temperature
2. For re-calibration pull the cap
outwards to expose the Calibration nut. (Fig. 17)
(20°C/68°F). Testing takes place according to DIN EN ISO 8655-6 with a completely assembled
(Fig. 17)
instrument and with uniform and smooth dispensing.
3. Using the calibration tool, turn the
calibration nut clockwise to reduce the
ISO 8655
Error Limits
±% ± ml
volume and anticlockwise to increase
±% ± ml
the volume. Repeat this procedure
(Fig. 18)
till the desired volume is achieved on the
0.25-2.5 ml
0.05 ml
0.5-5 ml
0.1 ml
1-10 ml
0.2 ml
2.5-30 ml
0.5 ml
5-60 ml
1.0 ml
10-100 ml
2.0 ml
electronic balance. (Fig. 18)
Maintenance / Cleaning
Unscrew the Calibration Nut with the
help of calibration tool to dis-assemble
The Dispenser should be cleaned in the following situations :
Immediatley when the piston is difficult
Prior to autoclaving.
the Piston and shaft out of the main
to move.
Prior to changing the valve.
housing. (Fig. 20)
Before changing the reagent.
Regularly when using liquids which form
Prior to long term storage.
deposits (e.g. crystallizing liquids).
Prior to dismantling the instrument.
Regularly when liquids accumulate in the cap.
(Fig. 20)
After unscrewing pull out the shaft. (Fig. 21)
All maintenance should be carried out wearing suitable eye protection and protective
(Fig. 21)
clothing. If in doubt, consult your safety officer.
Rinse the piston and shaft with deionized water. (Fig. 22)
1. Make sure that the Dispenser is completely empty.
2. Place the instrument into an empty sink together with its reservoir.
3. Unscrew the threaded platform base from the reservoir and lift the dispenser's intake
tube carefully out of the reservoir, whilst tapping it against the reservoir's aperture to
(Fig. 22)
shake off any droplets from the intake tube.
4. Hold the dispense nozzle over the aperture of the reservoir and apply gentle piston
strokes in order to return any contents into the reservoir.
5. Empty the instrument completely and flush thoroughly with distilled water.
6. If the piston barrel is still not completely clean, you need to dis-assemble the dispenser.
Clean the cylinder with a bottle-brush. If necessary
carefully remove deposits at the edge of the glass
cylinder. (Fig. 23)
Refer Dis-assembling procedure given below.
(Fig. 23)
Then flush all the parts of the instrument with deionized
water. (Fig. 24)
Dis-assembling the dispenser for cleaning and servicing :
1. Procedure to dis-assemble the piston:
(Fig. 24)
Pull the cap outwards to expose the Calibration Nut.
Insert the piston completely into the cylinder and then
(Fig. 19)
reassemble the instrument using the calibration tool
by screwing back the piston. (Fig. 25)
(Fig. 19)
(Fig. 25)
Snap back the cap to complete the assembly. (Fig. 26)
This instrument is autoclavable at 121° C ) (250° F) 1 bar absolute (15 psi)
2. Procedure to dis-assemble the DELIVERY PIPE
Unscrew the chuck nut and pull out the delivery pipe.
(Fig. 27) & (Fig. 28)
(Fig. 26)
(Fig. 27)
with a holding time of at least 15 minutes.
Note :
Only the piston needs to be removed for autoclaving the instrument.
Dis-assembling for Autoclaving :
Pull the cap outwards to expose the Calibration Nut.
Clean the delivery pipe with deionized water.
(Fig. 31)
(Fig. 28)
(Fig. 31)
Unscrew the Calibration Nut with the
3. Procedure to re-assemble the DELIVERY PIPE
help of calibration tool to dis-assemble
the Piston and shaft out of the main
housing. (Fig. 32)
(Fig. 32)
After unscrewing pull out the shaft. (Fig. 33)
First push the delivery pipe into the lower housing till
it stops going in further. (Fig. 29)
(Fig. 29)
(Fig. 33)
This is the piston-shaft sub-assembly.
Screw the chuck nut to complete the assembly.
(Fig. 30)
(Fig. 34)
(Fig. 34)
(Fig. 30)
Autoclave the two sub-assemblies at 1210C and
15 psi pressure for 10-15 mins. (Fig. 35)
Possible Cause
Piston Difficult
to move
Formation of crystals, dirty
Stop dispensing immediately.
Loosen piston with circular
motion, but do not disassemble.
Follow all cleaning instructions.
(see page 15 )
Air bubbles appear
in the Instrument
Reagent with high vapor
pressure has been drawn
in too quickly
Slowly draw in reagent.
The instrument has not
been primed
Prime the instrument.
(see page 11)
Filling tube is loose or
Push the filling tube on firmly.
if necessary cut off approx. 1 cm of
the tube at the upper end and then
re-connect it or replace filling tube.
Snap back the cap to complete the assembly.
Liquid reservoir is empty
Refill reservoir and prime unit.
(Fig. 37)
Too fast filling action
Fill and dispense slowly.
Leaking Piston
Clean Piston. (see page 15)
If problem persist replace
Leaking discharge valve
Clean by flushing throughly
with distilled water.
(see page 15)
Blocked Dispense
Disassemble the dispense
nozzle and flush through
with distilled water.
Clean Unit by immersing valve
assembly in distilled water.
(see page 15)
(Fig. 35)
Re-assembling after Autoclaving :
Insert the piston completely into the cylinder and then
reassemble the instrument use in the calibration tool
by screwing back the piston. (Fig. 36)
(Fig. 36)
(Fig. 37)
Dispensing not
Dispenser is now ready for use.
Discharge valve stuck
No Re-calibation is required after autoclaving.
However, a quick calibration check is recommended.
(Fig. 38)
Wrong Dispenser
Instrument not calibrated
Follow steps of user calibration.
(see page 14)
Barrel does not
fill with liquid
Inlet tube not fitted
Connect inlet tube correctly.
(see page 10, Fig. 2)
Filling Not Possible
Volume adjustment to
Minimum setting
Set to required volume.
(see page 12)
(Fig. 38)
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