Boekel Slide Moat Operating Instructions

Boekel Slide Moat
Model
240000 & 240000-2
Operating Instructions
N2400080 Rev. 2
Table of Contents
1.0
2.0
3.0
Safety ............................................................................................3
Product Information ...................................................................4
Assembly.......................................................................................4
4.0
Operation .....................................................................................4
5.0
6.0
7.0
8.0
3.1 Unpacking the Unit ............................................................................ 4
3.2 Installation ........................................................................................... 4
4.1
4.2
4.3
4.4
4.5
Identification of Controls (See Figure 1) ......................................... 4
Preparing the unit .............................................................................. 5
Loading the unit ................................................................................. 5
Removing the samples ...................................................................... 5
Setting the Temperature ................................................................... 5
Technical Specifications ............................................................5
Fault Diagnosis .............................................................................6
Accessories ..................................................................................6
Maintenance and Service ........................................................7
8.1 Cleaning .............................................................................................. 7
8.2 Replacement of Fuses ....................................................................... 7
9.0 Warranty .......................................................................................7
10.0 Service ..........................................................................................7
1
Figure 1
Front Panel
Heat Temperature
Display
Indicator
MODEL 240000
POWER
TEMPERATURE
Power
Switch
Figure 2
Rear of Unit
Fuse
Drawer
2
1.0
Safety
The following symbols marked on the equipment mean:
Caution: Read these operating instructions fully before use and pay
particular attention to sections containing this symbol.
Attention: Suivre attentivement les instructions avant l’usage et
prêtez une attention particulière aux sections comportant ce
symbole.
Caution: Surfaces can become hot during use.
Attention: Les surfaces peuvent devenir brûlantes pendant l’usage.
Always observe the following safety precautions :
•
•
•
•
•
•
•
•
•
•
•
Use only as specified by the operating instructions or the intrinsic
protection may be impaired. After transport or storage in humid
conditions, dry out the unit before connecting it to the supply
voltage. During drying out the intrinsic protection may be
impaired.
Connect only to a power supply that provides a safety ground
terminal.
Before moving, disconnect at the power supply socket. Do not
remove the plug.
Do not check temperature by touch. Use the temperature
display or a thermometer.
To reduce the risk of eye injury during high temperature
operation, use safety goggles or spectacles.
Do not touch surfaces that become hot during high
temperature operation.
Ensure that the operating temperature is less than the maximum
operating temperature of your sample material.
Ensure that the power switch is easily accessible during use.
Do not block or restrict ventilation slots.
If liquid is spilled inside the unit, disconnect it from the power
supply and have it checked by a competent person.
It is the user’s responsibility to carry out the appropriate
decontamination if hazardous material is spilled on or inside the
equipment.
3
2.0
Product Information
The Boekel Slide Moat is designed to provide a heating platform
and chamber capable of maintaining either dry or highly humid
conditions for In-Situ Hybridization applications. Uses include in-situ
RNA amplification, reverse transcription reactions, hybridization and
DNA melt.
The Boekel Slide Moat can hold up to 30 standard microscope
slides. The unit has an advanced PID temperature controller and
high density heating plate for providing very stable and accurate
temperatures from ambient plus 5˚C to 100˚C. The unit also
provides eight humidity generation wells which can be used to
create a humid environment for 12 or more hours. The unit comes
with a see-through tempered glass lid for observing reactions and
verifying humidity conditions.
There is sufficient clearance
(13/32”/1cm) between the heat plate and the glass lid bottom to
allow for the use of most in-situ cell carriers and for adding a flat
surface temperature gage to verify slide temperature.
Note: Boekel strongly recommends using covered and sealed slides
for higher temperatures (> 37° C) or prolonged period incubations
(> 1 hour).
3.0
Assembly
3.1
Unpacking the Unit
Remove the packing materials carefully, and retain for future
shipment or storage of the unit. Inspect for damage. Report all
shipping damage to the carrier immediately. Shipping damage is
covered by the carrier and repair/replacement for shipping
damages must be coordinated through the carrier. Complete and
return the Warranty Registration Card. Packages should contain:
• Slide Moat
• Glass Lid
• Power Line Cord
• Operating Instructions
3.2
Installation
Place the oven on a flat and stable surface, preferably away from
drafts. Do not block the ventilation slots on each side of the unit. Fit
the power line cord into the IEC power socket on the rear of the
unit.
4.0
Operation
4.1
Identification of Controls (See Figure 1)
4
The Power Switch controls power to the unit.
The Heat Indicator is on continuously while the oven is heating up.
As the required temperature is approached, it starts to flash. When
the unit is controlling at the set temperature, the heater lamp
flashes intermittently.
The Temperature Display shows the chamber temperature in
degrees Celsius.
4.2
Preparing the unit
Switch the unit on (I) and set the PID Controller to the desired
operating temperature. Although the unit heats rapidly, you may
want to first pre-heat the unit to the desired operating temperature
before incubating samples. If a humid chamber is desired, fill each
of the eight humidity wells with 0.1ml of distilled water.
4.3
Loading the unit
Place slide samples on the land areas of the heat plate. Place the
tempered glass lid on the gasketed perimeter of the heat chamber.
Secure the glass lid by gently depressing the lid one corner at a
time and rotating the plastic keeper over the lid edge. As you
release the glass lid it will secure itself against the keeper. If water is
being used, you will notice a slight fogging above each well as the
unit generates and maintains the humid environment.
4.4
Removing the samples
Caution! The lid may be hot. Make sure the lid is cool before
attempting to remove keepers. Release lid keepers by again
depressing the lid at each corner and rotating the keeper off the
lid. Remove the glass lid. Caution! The heating plate may be hot.
Allow the heating plate and slides to cool before removing the
slides.
4.5
Setting the Temperature
The temperature controller has three buttons. When the button on
the left, ‘*’, is depressed, the set temperature is displayed. When
the left button ‘*’ is depressed simultaneously with the middle
button ‘’, the set temperature value is lowered. When the left
button ‘*’ is depressed simultaneously with the right button ‘’, the
set temperature value is raised. When all buttons are released, the
actual chamber temperature is displayed.
5.0
Technical Specifications
This equipment is for indoor use and will meet its performance
figures within the ambient temperature range of 10°C to 35°C, with
maximum relative humidity of 80% non-condensing. Installation
category II (transient voltages). Pollution degree 2 in accordance
with IEC 664. For operation at altitudes of up to 6500 feet.
5
Temperature Range
Temperature Setting Range
Stability
Uniformity
Temperature Display
Resolution
Supply Ratings
Power Rating
Heating Rate
6.0
Ambient + 5°C to 100°C
10.0°C to 100.0°C
±0.2°C throughout operating temperatures
±0.35°C at 37°C; ±0.7°C at 65°C
0.1°C
Model 240000: 115 VAC, 1.74 A, 60 Hz
Model 240000-2: 230 VAC, 0.87 A, 50/60 Hz
Model 240000: 200 W
Model 240000-2: 200 W
Ambient to 65°C in 20 minutes
Fault Diagnosis
Symptom
Unit does not operate
Possible Cause
Action Required
a. Unit not switched on a. Switch on
b. Unit not plugged
b. Plug in, switch on
into power supply
c. Fuses blown
c. Replace fuses (see
section 8.2)
d. Power supply failure d. Check that other
electrical appliances
on the same circuit
are working
Chamber temperature
does not rise when
expected
a. Actual temperature
is higher than set
temperature
b. Temperature
control circuit fault
a. Check set
temperature
Temperature continues
to rise when not
expected
a. Actual temperature
is lower than set
temperature
b. Temperature
control circuit fault
a. Check set
temperature
7.0
Accessories
Part Number
C1303205
Description
Light Shield Cover
6
b. Have unit checked by
a competent service
person
b. Have unit checked by
a competent service
person
8.0
Maintenance and Service
All Boekel laboratory products are designed to comply with
IEC1010-1. No routine maintenance is required.
8.1
Cleaning
Disengage power cord prior to cleaning. If a spill occurs, use
appropriate clean-up procedures as required for radiation or
biohazard control. The outer casing can be cleaned with a cloth
dampened with water. Do not immerse the unit in water.
8.2
Replacement of Fuses
There are two supply fuses located in the fuse drawer. To change
the fuses:
• Turn power switch to the off (O) position
• Disconnect the unit from the power supply
• Remove the line cord from the power entry module on the back
of the unit
• Pull back on the fuse drawer catch
• Pull out the fuse drawer
• Check and replace with the correct fuses if necessary. The
fuses should be 5mm x 20mm quick acting, rated 250V.
Model 240000 (115 V AC):
2AF
Model 240000-2 (230 V AC):
1AF
• Push the fuse drawer back in. Reconnect unit to power supply.
9.0
Warranty
When used in laboratory conditions and according to these
operating instructions, Boekel warrants this product to be free of
defective material and workmanship for a period of two years from
the date of manufacture. The liability of Boekel for any defective
equipment during the warranty period shall be limited to the repair
of such equipment or replacement thereof without charge for parts
or labor.
10.0 Service
It is required to obtain a Returned Material Authorization (RMA)
number before any Boekel products are returned for any reason. A
Decontamination Certificate must be completed, signed by the
user, and returned to Boekel Scientific prior to receiving the RMA
number. Please be sure to mark the outside of the returned goods
package with this RMA number to ensure prompt handling
7
Boekel Scientific
855 Pennsylvania Boulevard
Feasterville, PA 19053
Phone: (215) 396-8200 or (800) 336-6929
Fax: (215) 396-8264
e-mail: boekel-info@boekelsci.com
8

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