Mass Directed Flash Chromatography by Biotage

Mass Directed Flash Chromatography by Biotage
Mass Directed Flash
Chromatography by Biotage
Biotage Dalton Mass Detectors
®
Why Do Companies Around the World Choose
the Biotage Approach to Flash Purification?
As a pioneer of automated Flash purification, Biotage counts many major
companies as customers. These organizations have selected Biotage as a partner
because they receive the fastest and most cost efficient route to compound
purification. They enjoy purification done right the first time, every time.
Here at Biotage we are committed to developing tools for
a seamless workflow that gets projects to completion. Our
Accelerated Chromatographic Isolation (ACI) enabled Isolera
systems and SNAP Ultra flash cartridges deliver the most
reliable and rapid purifications possible.
Flash Purification with Mass Detection
Why choose Flash Chromatography with integrated mass
identification?
Benefits include:
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Immediate identification of compounds
Samples separated by mass-to-charge ratio
Sample crude mixtures directly
Isolera™ Archive Report - 2017-May-11 10.39
User
Sample Name
Date
Method
Cartridge
Flowrate
Solvent A
Solvent B
Chemist
2017-May-11 10.39
2017-May-11 10.44
Rack Type
Max Fraction Volume
Dispense Order
18x150 mm
27 ml
S
SNAP Ultra C18 12g
12 ml/min
Water
Acetonitrile
Detection Mode
m/z Range
XIC m/z
Baseline Correction
Lambda-all (Monitor)
1.
XIC
100.0 - 300.0 (APCI)
(-)151.0, (-)179.0, (-)193.0
On
(Brown)
Identification of the mass of eluting compounds as they are
isolated provides the information you want, when you want it.
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Isolera Mass Directed Flash Chromatography | © Biotage 2017
Biotage Coupling Technologies
The key to success in mass-directed flash purifications is integrating the high
flow rates and broad variety of organic solvents used in flash with the low sample
volumes required by mass spectrometers. To achieve this, Biotage has developed
Isolera Spektra enhanced detection and Isolera Nanolink. These reliable and robust
technologies simplify the day-to-day operation of Biotage Dalton systems.
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Industry standard
switching valve
Three solvent inlets for
positive, negative and
neutral makeup solvents
Precision
machined piston
solvent pumps
Isolera Spektra
Biotage Nanolink
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Enabling Isolation of
Pure Compounds
Integrating Mass Detection
with Flash Purification
Isolera Spektra is a powerful software for Isolera flash
systems, combining instrument control with extended features
for flash purification.
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Features
»» Baseline correction
»» Advanced λ-All detection
»» Preview your run in the built-in simulator
»» PDA spectral analysis
»» MS analysis capability through a wizard interface
»» Automatic handling of mass spectrography parameters
»» Allows direct mass spectrography analysis of crude mixtures
An intelligent coupling system which ensures worry-free and
reliable hyphenated operation between the flash chromatograph and mass detector. Nanolink is fully automated – there’s
no need for manual adjustments during setup or operation.
Features
»» Eliminates flow splitter optimization
»» Automatic sampling
»» Automatic flow adjustment
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m/z Range 10–2000.
Supports atmospheric pressure
chemical ionization (APCI) and
electrospray ionization (ESI) of the
sample.
Collect on TIC enables you to
collect and fractionate based
on all compounds seen by the
mass detector.
Industry standard direct
injection port ensures rapid
and precise sample analysis.
A single quadrupole analyzer filters
the ions according to their massto-charge ratios (m/z) and directs
them into the detection system.
Automatically switches between
positive and negative ionization.
Biotage Dalton 2000
®
Isolera Dalton 2000
™
Full mass spectral analysis capability including
post-run analysis, collection based on TIC, and
polarity switching during the run.
Applications
»» Mass directed purifications of all types of reaction mixtures
»» Purification of known samples
»» m/z Information from purification of unknown compounds
»» Natural product analysis
Quick Facts
»» m/z Range 10–2000
»» Collect and fractionate based on total ion
»»
»»
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count or extracted ion signals
APCI ion source (ESI optional)
Full spectral analysis post run
ASAP Probe for liquid and solid phase analysis
External roughing pump
Isolera Dalton 2000 with Biotage Dalton 2000 Mass
Detector, Isolera Four flash system, Biotage Nanolink and
external roughing pump.
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Isolera Mass Directed Flash Chromatography | © Biotage 2017
Miniaturized mass analyzer optimized for use
in flash chromatography, providing instant
confirmation of the collected fraction’s mass.
m/z Range
80–800 m/z
Small footprint,
fits in a regular
fume hood.
Internal vacuum
pumps with 30 minute
pump down time.
Isolera Dalton Mass Detector
™
Isolera Dalton
™
Miniaturized system for laboratories
where space is a premium.
Applications
»» Mass directed purification of simple reaction mixtures
»» Purification of known samples
»» Small sample volumes
Quick Facts
»» Miniaturized design
»» Internal roughing pump
»» m/z Range 80–800
»» Collect and fractionate based on up to four masses per run
»» Nano-ESI ion source
Learn More
Isolera Dalton - Flash Chromatography with
Integrated Mass Identification
™
Isolera Dalton System with Isolera Dalton
Mass Detector, Isolera One flash system, and
Biotage Nanolink.
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Technical Specifications
Biotage Dalton 2000
Isolera Dalton Mass Detector
Dimensions (w x d x h)
28 x 56 x 66 cm
35.5 x 56 x 25 cm
Weight
54 kg
32 kg
Ion source
APCI (ESI optional)
ESI nano spray
Flow rate range
Flash separation at 10–100 mL/min
Flash separation at 10–100 mL/min
Sampling adjustment
Automatic split flow interface
Automatic split flow interface
Acquisition modes
Positive and negative ionization in parallel
Selectable positive or negative ionization
Detection range (m/z)
10–2000
80–800
Resolution
< 0.7 FWHM m/z
0.7 ±0.1 FWHM m/z
Detection accuracy
±0.1 m/z
±0.5 m/z
Mass fractionation limit
4
4
Make up solvents
One (pure)
Three (mixtures)
Nitrogen gas pressure
4.1–6.9 bar (60–100 psi), minimum 98% purity
2–6 bar (29–87 psi), minimum 99.9% purity
Gas consumption
8 L/min
2.5 L/min
Accessories
ASAP direct probe analysis
N/A
Custom mass intervals
Yes
N/A
Collect on TIC
Yes
N/A
Automatic stop on target mass
Yes
N/A
Post run analysis
Built into software and offline
Offline only
Vacuum pumps
Internal turbo pump, external rotary vane roughing
pump
Integrated turbo and foreline roughing
vacuum pump
Control interface
Isolera Spektra
Isolera Spektra
User serviceable parts
Heated inlet capillary with o-ring and Corona needle
spraychip (chip based ESI source), vac-chip
Certifications
CE, TÜV, FCC
CE, cTÜVus
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Isolera Mass Directed Flash Chromatography | © Biotage 2017
Ordering Information
Description
Part Number
Mass Detectors
Biotage Dalton 2000 Mass Detector
APCI 230 VAC incl roughing pump
DALT-2000-APCI-230V
Biotage Dalton 2000 Mass Detector
APCI 115 VAC incl roughing pump
DALT-2000-APCI-115V
Biotage Dalton 2000 Mass Detector
APCI 100 VAC incl roughing pump
DALT-2000-APCI-100V
Biotage Dalton 2000 Nanolink
DALT-2000-NLINK
Isolera Dalton Nanolink
ISO-DALT-NLINK
Isolera Dalton Mass Detector
ISO-DALT-DET
Spektra License for Dalton 2000
DALT-2000-SW
Description
Part Number
Exterior tubing kit (without SFI tubes
and solvent inlet tubes)
413875
Solvent inlet tubing with filter
(pack of 3)
413460
Tubes, 20x150mm, for cleaning the
vac-chip
413890
DFI, Direct Flow Interface cpl.
413743
SFI, Split Flow Interface (Includes
tubing for 1ml/min split)
413741
MID 4100 Calibration kit
ISO-DALT-CALIB
™
Mass Detector Accessories
Isolera One
Isolera One, One Channel, Single
Collection Bed, 200-400 nm Detector
ISO-1SV
Isolera One, One Channel, Single
Collection Bed, 200-800 nm UV-VIS
Detector
ISO-1SW
Isolera One, One Channel, Extended
Collection Bed, 200-400 nm Detector
ISO-1EV
Isolera One, One Channel, Extended
Collection Bed, 200-800 nm UV-VIS
Detector
ISO-1EW
Dalton 2000 ASAP kit, probe, capillary
stand, APCI source port
UP-ASAP-SNL
Dalton 2000 APCI Ion source
IS-APCI-S01
Dalton 2000 ESI Ion source
IS-ESI-S01
Dalton 2000 Glass capillaries for ASAP,
pk 100
CAP-ASAP
Vac-chip 100
413712
Spraychip 200
413711
Masscape SW for Personal Computer
413874
Isolera Four
Vac-chip O-ring (Pack of 4)
413872
413710
Isolera Four, Four Channels, Single
Collection Bed, 200-400 nm Detector
ISO-4SV
250ul gastight syringe
Microfilter for make up flow (pack of 5)
413484
413483
Isolera Four, Four Channels, Single
Collection Bed, 200-800 nm UV-VIS
Detector
ISO-4SW
Microfilter holder with 2m filter
(pack of 2)
Needle Port (pack of 2)
413487
ISO-4EV
Splitter maintenance kit (pack of 3)
413714
Isolera Four, Four Channels, Extended
Collection Bed, 200-400 nm Detector
Isolera Four, Four Channels, Extended
Collection Bed, 200-800 nm UV-VIS
Detector
ISO-4EW
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Title Truncated if Neccessary... | Page 8
Your Complete
Partner for Effective
Chemistry
Biotage is a worldwide supplier of
instruments and accessories designed
to facilitate the work of laboratory and
process chemists. With our deep knowledge
of the industry, academic contacts and
in-house R&D teams, we can deliver the best
solutions to your challenges. We take great
pride in our flexibility and ability to meet our
customer’s individual needs. With strong
foundations in both analytical, organic and
process chemistry, we can offer the widest
range of solutions available on the market.
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Order Tel: +46 18 565710
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Order Fax: +46 18 565705
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Support Tel: +1 800 446 4752
Support Fax: + 46 18 56 57 11
Outside US: +1 704 654 4900
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Part Number: PPS450
© 2017 Biotage. All rights reserved. No material may be reproduced or published without the written permission of Biotage.
Information in this document is subject to change without notice and does not represent any commitment from Biotage. E&OE.
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© Biotage 2017
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