Operating Manual MyGel™ Mini a division of

Operating Manual MyGel™ Mini a division of
6 Troubleshooting (continued)
Problem
Causes
Resolutions
Error E1
Lid opened during run or
magnet switch problem.
Seat lid properly. Check
magnet on lid..
MyGel™ Mini
Error E3
Lid not closed upon start
or magnetic switch
problem.
Seat lid properly. Check
magnet on lid.
Electrophoresis System
Check proper buffer level in
the tank. Check buffer
concentration (1X or 0.5X
TAE or TBE is standard).
Check electrodes and
connections.
Operating Manual
Error E5
Electric current not
flowing normally. Buffer
missing, or concentration
too high.
Error E7
Buffer concentration too
high, current too high,
power over 50W, or
internal electronic
problem
Check buffer concentration.
Replace electronic board.
a division of
Version 1.2
Contents
1. Introduction……………………………………….....................1
1.1 Included Component List…….………….…………………..1
1.2 Component Diagram...………………..................................2
1.3 Specifications.………………………….…............................3
2. Installation….…………………………………..…………..……3
3. Warnings……………..………………………...........................5
4. Operating Guide.……………………………….…………..…..5
4.1 Control Panel Key Functions……….………....……..…….5
4.2 Gel Preparation….…..…………………...…........................ 5
4.3 Electrophoresis …………….…………………….…..………7
5. Maintenance
Always disconnect the power cord for cleaning.
When cleaning the surfaces of the instrument, use a
damp cloth with mild detergent if needed. Do not
use any corrosive solutions that could damage
plastic..
When cleaning the gel tank, first separate the Power
Supply. Use water or neutral cleaner.
Use caution them cleaning the areas near the
platinum electrodes at the bottom edges of the gel
tank.
Clean gel casting stands, gel tray and combs
with water or a neutral cleaner.
6. Troubleshooting
Problem
5. Maintenance……………………………………….……..……..9
No display
Check power supply,
Unplug and re-attach
power cord. Check fuse.
Contact Benchmark for
repair.
No Migration
Power switch
off, no power
Turn on power switch.
Contact Benchmark for
repair.
Abnormal
Electrophoresis
Distance
Incorrect input
voltage
Check main power
supply.
Key function
failure
Control panel
switch failure
Contact Benchmark for
repair.
Thank you for purchasing the Accuris MyGel™ Mini
Electrophoresis System.
Please check the packing list when first opening the box,
and if there are any parts missing, damaged, or incorrect,
please contact your distributor or Benchmark Scientific’s
Customer Service Department at 908-769-5555 or by email
info@benchmarkscientific.com.
Resolutions
Power not
connected.
Switch failure.
Fuse blown.
Controller
failure.
6. Troubleshooting………………….…..…................................9
This operating manual includes a product introduction,
operating and safety information. Before using the MyGel,
please read this manual in its entirety and be sure to fully
understand the features and methods for proper operation.
Keep this manual for future reference.
Causes
9
1. Introduction
Use an appropriate pipette to
carefully dispense samples into
the wells of the gel.
The MyGel is a complete horizontal electrophoresis
system that includes a power supply that directly
connects to a gel tank. The system is designed for
separating nucleic acids in agarose gel. All components
and accessories are included for casting and running
small gels.
Attach the lid. The display screen will light
when the lid is properly seated and the power
switch is turned on.
Before use, please read this operating manual in its
entirety.
Press to set the run time from 1 to
99 min. For a continuous run, set the
timer to “00”.
Press the Voltage Selection button
select the appropriate output voltage.
1.1 Included Components
to
Press the “Start/Stop” button once to start a run. The set
output voltage LED will blink to indicate a run is in process. At
the end of a timed run, the alarm will beep 3 times, and the
display will show “Ed” to indicate END. Press any key to clear
“Ed” and set up another run.
To stop a run in process, press and
hold “Start/Stop” for 3 second. The
voltage LED will stop blinking to
indicate the run has stopped.
Indication
light
Power Supply and Tank Assembly
1 pc
Power cord
1 pc
Gel Casting Stand (A)
1 pc
Gel Casting Stand (B)
1 pc
Gel Tray (A)
2 pcs
Gel Tray (B)
4 pcs
Combs (A)
2 pcs
Combs (B)
2 pcs
AC transformer *
1 pc
User Manual
1 pc
* Note: only model “MyGel –E” includes the AC
transformer.
At the end of an electrophoresis run, turn off the power
and open the lid to remove the gel tray.
8
1
1.2 Component Diagram
4.3 Electrophoresis
Safety
Cover
Note: Make sure that the MyGel System is
installed on a stable and level surface to
ensure even sample migration.
Start/Stop
Place a prepared gel, together with the gel
tray onto the gel bed inside the gel tank.
Gel Tray
Timer
setting
Voltage
selection
Power
switch
Power
Supply
Pour an appropriate buffer solution to a
level about 1 to 3mm above the surface of
the gel. Generally, 250-300ml of buffer is
required.
Platinum
electrode
Connect the power supply to the
migration tank and insert the electric plug
into an outlet. The electric current cannot
be turned on with the lid open.
Power inlet
Gel tank
Fuse
2
7
Migration
tank
1.3 Product Specifications
Pour the agaose gel solution into
the gel tray to make a gel
approximately 4mm thick. Each
60x110mm tray requires about
25ml of agarose.
Model
Note: After mixing and heating
agarose solution, let it cool to
approximately 60 C before pouring
into the gel trays
After the gel has solidified
(approximately 20 minutes) hold
the two sides of comb and gently
lift it out of the gel. The wells
should be straight and
undamaged.
MyGel Mini
110V
MyGel Mini
230V
Output voltage
DC35V , DC50V, DC100V
Timer
0 ~ 99min
Migration Tank
120mm×110mm×45mm(inner dimension)
Buffer Volume
250ml-300ml
Comb Specs
Teeth width x Teeth thickness x Teeth no.)
3mm×1mm×22 / 5.6mm×1mm×12
3mm×1mm×18 / 5.6mm×1mm×10
Gel Casting
Stands
10.5cm×6cm and 5cm×6cm (Dimension for
each section)
Gel tray Specs
10.5cm×6cm and 5cm×6cm
Fuse
250V 2A
Dimensions
190mm×130mm×55mm (W×D×H)
Net weight
0.45 kg(without AC transformer)
2. Installation
Remove the gel tray with the gel
from the stand and place it into the
migration tank in the correct
orientation. (DNA samples will
migrate from the – to + electrode.
Place the electrophoresis system on a smooth, level surface.
Connect one end of the power cord to the instrument and the
other end to an appropriate outlet, the input voltage should be
between 100~120VAC.
When the input voltage is 220VAC, use the transformer inline.
6
3
3 Warnings
4. Operating guide
To avoid electrical shock, do not use this product
with wet hands.
When operating, do not move or bump the system,
do not put your finger or any other objects into the
migration tank.
4.1 Key Functions
Start/Stop----Press momentarily to start a run. Press and hold for
3 seconds to stop a run.
---------Output voltage selection.
 -----------Time setting. For continuous run, set to “00”
Do not detach the power supply module from the
migration tank when the power is on. Attempting to
do so can cause damage.
Please carefully read this instruction manual before
operation to avoid any personal injury. Only trained
laboratory personnel should operate the system.
Do not attempt to open or repair the MyGel system.
Contact your distributor or Benchmark Scientific for
service.
Always use the MyGel in an environment with low
humidity and low dust, also keep it away from water,
direct sunlight / strong light, corrosive gas, high
magnetic fields, heaters, fires and other heat sources.
The power switch is located on the control box,
press “I” to power on, “O” to turn off.
Always turn off the power after operation. When not in
use, detach the power supply and store it in an area that
is free from moisture and dust.
4
4.2 Gel Preparation
Gel tray
Place the gel casting stand on a
level surface, and place the gel
trays into the proper positions in
the stand.
Note: If the gel tray is not level,
the thickness of the gel will not
be uniform and migration may be
uneven.
Insert the comb into the gel
casting stand as per photo on the
right.
Note: when using the fine toothed
comb, use the orientation so the
flat side of the well is facing the
direction of DNA travel. See
figure below.
5
Gel
stand
Comb
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