Thermo Scientific NanoDrop 2000

Thermo Scientific NanoDrop 2000
KHRBB Guide
Thermo Scientific NanoDrop 2000
Instrument overview
The Thermo Scientific NanoDrop 2000 is a micro-volume spectrophotometer that allows for
samples to be measured at volumes of 1 - 2 µL. The instrument can be used to measure DNA,
RNA and protein (A280, Bradford, BCA, Lowry and Pierce 660 nm) concentrations and also
sample purity (260/280) ratio in less than 5 seconds per sample. The instrument has a large
concentration range: 2 ng/µL – 15,000 ng/µL dsDNA and comes with user-friendly software that
analyzes and exports data. The instrument does not require special consumables and therefore
has a low cost of operation.
Rules for using the Thermo Scientific NanoDrop 2000
The NanoDrop 2000 is an extremely easy instrument to use and training takes approximately 15
minutes. Once competency is established, users can on-line book and use instrument, making
sure that they leave the instrument in a good, clean, DNA/RNA free state.
1. Contact James Thompson to discuss your project and to introduce the instrument and
highlight instrument advantages and disadvantages.
2. Read sample NanoDrop 2000 user manual (copy available from KHRBB)
3. During training user will learn how to:

open software and select measurement application of interest

set up measurement report so that all data points are automatically saved

clean measurement pedestal using damp lint-free laboratory wipes

establish a blank value using appropriate buffer

measure sample

wipe pedestal using dry lint-free laboratory wipes

after final sample measurement run a blank cycle

clean pedestal with double distilled H2O

analysis and export data using software features
4. Sign user agreement and agree about user competency
5. Run experiments on own, reserving equipment via on-line booking system
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