Agilent 1290 Infinity II Online SPE Solution Instruction

Agilent 1290 Infinity II Online SPE Solution Instruction
Agilent 1290 Infinity II
Online SPE Solution
Instruction
Agilent Online-SPE-Solution - Instruction
Technical Information about the Agilent Online-SPE-Solution (optional setups with:
G4242A Online-SPE Starter Set, G4743A Online-SPE Multi Cartridge Kit, G4744A
Online-SPE Direct Inject Kit, G4745A Online-SPE High Volume Injection Kit)
Contents
Typical Applications
2
G4742A Online-SPE Starter Kit 2
G4744A Online-SPE Direct Inject Kit 3
G4743A Online-SPE Multi Cartridge Kit 5
Delivery Checklist
7
Online-SPE-Solution Setup
9
Setting up the Starter Kit G4742A 9
Setting up the Direct Inject Kit G4744A 11
Setting up the SPE Multi Cartridge Kit G4743A
Installing the Valve Heads
13
15
Valve Head Parts Information
19
Agilent Technologies
Typical Applications
G4742A Online-SPE Starter Kit
Typical Applications
G4742A Online-SPE Starter Kit
The Agilent 1290 Infinity II Online SPE Solutions are based on the 1290 Infinity
Flexible Cube that houses re-usable SPE (solid phase extraction) cartridges and up to
two valves. Combined with the Agilent 6400 Series Triple Quadrupole mass
spectrometer the 1290 Infinity II Online SPE Solution allows ultra-low, trace-level
detection.
The G4742A Online-SPE Starter Kit builds the basis for all possible Online-SPE
solutions.
Figure 1
2
Flow path in an Online-SPE Starter Kit setup
Typical Applications
G4744A Online-SPE Direct Inject Kit
G4744A Online-SPE Direct Inject Kit
In addition to the Online SPE Starter set G4742A the online SPE direct injection kit
G4744A allows also to bypass the SPE cartridges and instead inject directly onto the
analytical column.
Figure 2
Direct Injection (Valve A in Position 1)
Valve A (left) in position 2 connects the flow path to valve B (right). From valve B, SPE
cartridges can be chosen for either loading or elution.
3
Typical Applications
G4744A Online-SPE Direct Inject Kit
4
Figure 3
Valve A in Position 2 and Valve B in Position 1
Figure 4
Valve A in Position 2 and Valve B in Position 2
Typical Applications
G4743A Online-SPE Multi Cartridge Kit
G4743A Online-SPE Multi Cartridge Kit
The Agilent 1290 Infinity II Online SPE Solution offers a method development kit for
online SPE cartridges.
This Online SPE Multi-Cartridge Kit (G4743A) is based on the Online SPE Kit
(G4742A) and is recommended in combination with the Direct Injection Kit (G4744A).
It adds a column selection valve (6-position/14-port valve head, 600 bar) for SPE
method development. This 6-position/14-port valve is installed next to the 1290
Infinity Flexible Cube in an external valve drive. The Flexible Cube itself hosts two
2-position/10-port valves to support direct injections and online SPE.
Figure 5
Flow path of the direct injection run
The Agilent 1290 Infinity Flexible Cube offers the possibility of direct injections and
online SPE. In Figure 5 on page 5, the flow path of the direct injection run is shown. In
this position, the right 2-positon/10-port valve and the additional 6-column selection
valve in the external valve drive are disabled and not busy.
With the built-in solvent selection valve in the 1290 Infinity Flexible Cube, up to three
solvents can be used to condition and to regenerate the cartridges ( Figure 6 on
page 6). Also, the right 2-position/10-port valve can be switched between online SPE
and bypass, for cleaning the system.
5
Typical Applications
G4743A Online-SPE Multi Cartridge Kit
Figure 6
Flow path for online SPE
For online SPE, the flow path of the Agilent 1290 Infinity Flexible Cube goes through
the left and right 2-position/10-port valve and afterwards to the 6-column selection
valve. One out of the six cartridges is loaded with the sample.
Figure 7
Flow path to elute the loaded cartridge
To elute the loaded cartridge in the previous step (Figure 6 on page 6), the right
2-position/10-port valve is switched.
NOTE
6
Additional information can be found in Online SPE Multi-Cartridge Kit for Fast and Easy Method
Development - The Agilent 1200 Infinity Series Online SPE Solution (5991-4005EN).
Delivery Checklist
G4743A Online-SPE Multi Cartridge Kit
Delivery Checklist
Check the content of the delivery. You should have received the following:
G4742A Online-SPE
Starter Set
Online SPE
Capillary Starter
Kit (5067-4294)
G4744A Online-SPE
Direct Inject Set
Online SPE Direct
Inject Capillary Kit
(5067-4295)
p/n
Description
5067-4145
2pos/10port valve head 600 bar
5067-4294
Online SPE Capillary Starter Kit
#
p/n
Description
2
0890-1713
Tube PTFE, 2 m
2
0100-1516
Fitting male PEEK, 2/pk
1
5982-1271
BE online PLRP-S 15 – 20 µm2.1 mm x 12.5 mm 3/pk
1
G1329-87300
Capillary 0.17 mm, 900 mm
1
G1316-87319
Capillary ST 0.12 mm x 340 mm S/S
2
5067-4670
Capillary ST 0.17 mm ID 600 mm pre-swaged
1
5065-9979
Capillary ST 0.25 mm x 250 mm
5
01090-87308
Capillary ST, 0.25 mm x 130 mm
1
5065-9967
Fitting screw extra long
10/pk
1
G4742-90101
Online - SPE Kits Technical Note
1
G4742-60000
CD with online SPE method
p/n
Description
5067-4145
2pos/10port valve head 600 bar
5067-4295
Online-SPE Direct Inject Kit
#
p/n
Description
2
5043-0277
Plug Black Long 10-32
1
0100-1516
Fitting male PEEK, 2/pk
1
01090-87308
Capillary ST, 0.25 mm x 130 mm
1
5022-6508
Capillary, 0.25 mm x 280 mm no fittings
2
5021-1822
Capillary, 0.12 mm x 280 mm
1
G1316-87319
Capillary ST 0.12 mm x 340 mm S/S
1
G4742-90101
Online - SPE Kits Technical Note
1
G4742-60000
CD with online SPE method
7
Delivery Checklist
G4743A Online-SPE Multi Cartridge Kit
G4743A
Online-SPE Multi
Cartridge Set
Capillary Kit Online
SPE Multi Cartridge
(5067-4296)
8
p/n
Description
5067-4146
6pos/14port valve head
In combination with the G4744A Online-SPE direct Injection kit a 3rd valve
drive is needed, for example an additional G1170A
5067-4296
Online SPE Multi Cartridge Capillary Kit
#
p/n
Description
2
5067-5112
Capillary ST 0.17 mm x 280 mm SV/M
2
5067-5120
Capillary ST 0.17 mm x 700 mm SL/M
12
5067-5109
Capillary ST 0.17 mm x 90 mm SL/M
1
5023-2504
Hex driver SW-4 slitted
1
G4742-90101
Online - SPE Kits Technical Note
1
G4742-60000
CD with online SPE method
Online-SPE-Solution Setup
Setting up the Starter Kit G4742A
Online-SPE-Solution Setup
Setting up the Starter Kit G4742A
NOTE
This section describes the setup of the Starter Kit only. Refer for the following sections if used in
combination with complementary Online-SPE kits.
NOTE
More application details can be found in the Online - SPE Application Note (p/n 5991-1738EN).
Setup the hydraulic path of the Online – SPE Starter Set.
9
Online-SPE-Solution Setup
Setting up the Starter Kit G4742A
Table 1
10
Capillary Connections for the Starter Kit
Part Description
Connection
Capillary ST, 0.25 mm x 130 mm (01090-87308)
Valve - cartridges (4x)
Valve crossing (Port 5 – Port 10)
Capillary ST 0.12 mm x 340 mm S/S (G1316-87319)
Valve (port 2) - column
use long fittings on valve
Capillary ST 0.17 mm x 900 mm S/S (G1329-87300)
LC pump - valve (Port 7)
Capillary ST 0.17 mm ID 600 mm pre-swaged
(5067-4670)
Pressure Sensor - Autosampler
Autosampler - Valve (port 4)
Capillary ST 0.25 mm x 250 mm (5065-9979)
Flush pump - pressure sensor
(Use PEEK fitting for Flush pump)
Waste tubing, 2 m (0890-1713)
valve (port 9)
Online-SPE-Solution Setup
Setting up the Direct Inject Kit G4744A
Setting up the Direct Inject Kit G4744A
NOTE
The Direct Inject Kit can only be used in combination with the G4742A Online SPE Starter Kit.
Setup the hydraulic path of the Online – SPE Direct Inject Kit.
Figure 8
Direct Injection (Valve A in Position 1)
Use the capillaries and parts as described in Table 2 on page 12
11
Online-SPE-Solution Setup
Setting up the Direct Inject Kit G4744A
Table 2
12
Connecting the Online-SPE Direct Inject Solution by using capillaries from both kits, the
Online - SPE Capillary Kit (p/n 5067-4294) and Online - SPE Direct Inject Kit (p/n
5067-4295)
Part Description
Connection
Capillary, 0.25 mm x 280 mm no fittings (5022-6508)
Valve A / Port 4 - Valve B / Port 4
Capillary, 0.12 mm x 280 mm (5021-1822)
Valve A / Port 3 - Valve B / Port 7
Valve A / Port 7 - Valve B / Port 2
Capillary ST, 0.25 mm x 130 mm (01090-87308)
Pressure Sensor - Valve A / Port 10
Valve to cartridges (4x)
Valve crossing, Valve B (Port 5 – Port 10)
Capillary ST 0.12 mm x 340 mm S/S (G1316-87319)
Valve A (port 6) - column
Autosampler – Valve A / Port 5
use long fittings on valve
Capillary ST 0.17 mm x 900 mm S/S (G1329-87300)
LC pump to valve A (Port 2)
Capillary ST 0.17 mm ID 600 mm pre-swaged
(5067-4670)
Valve A (port 1) - Autosampler
Capillary ST 0.25 mm x 250 mm (5065-9979)
Flush pump - pressure sensor
(Use PEEK fitting for Flush pump)
Waste tubing, 2 m (0890-1713)
valve B (port 9)
Plug Black Long 10-32 (5043-0277)
Valve A (port 8, port 9)
Online-SPE-Solution Setup
Setting up the SPE Multi Cartridge Kit G4743A
Setting up the SPE Multi Cartridge Kit G4743A
NOTE
The Online SPE Multi Cartridge Kit can only be used in combination with the Online-SPE Starter Kit
(G4742A) and the Online-SPE Direct Inject Kit (G4744A).
Figure 9
Flow path of the direct injection run
13
Online-SPE-Solution Setup
Setting up the SPE Multi Cartridge Kit G4743A
Table 3
14
Capillary Connections for the Multi Cartridge Solution
Part Description
Connection
Capillary, 0.25 mm x 280 mm no fittings (5022-6508)
Valve A / Port 4 - Valve B / Port 4
Capillary, 0.12 mm x 280 mm (5021-1822)
Valve A / Port 3 - Valve B / Port 7
Valve A / Port 7 - Valve B / Port 2
Capillary ST, 0.25 mm x 130 mm (01090-87308)
Pressure Sensor - Valve A / Port 10
Valve crossing, Valve B (Port 3 – Port 6)
Valve crossing, Valve B (Port 5 – Port 10)
Capillary ST 0.12 mm x 340 mm S/S (G1316-87319)
Valve A (port 6) - column
Autosampler – Valve A / Port 5
use long fittings on valve
Capillary 0.17 mm, 900 mm (G1329-87300)
LC pump - valve A (Port 2)
Capillary ST 0.17 mm ID 600 mm pre-swaged
(5067-4670)
Valve A (port 1) - Autosampler
Capillary ST 0.25 mm x 250 mm (5065-9979)
Flush pump - Pressure sensor
(Use PEEK fitting for Flush pump)
Waste tubing, 2 m (0890-1713)
Valve B (port 9)
Plug Black Long 10-32 (5043-0277)
Valve A (port 8, port 9)
Capillary ST 0.17 mm x 280 mm SV/M (5067-5112)
Valve B (port 1) - Selection valve 'IN' port
Selection valve 'OUT' port - Valve B (port 8)
Capillary ST 90x 0.17 mm SW-1/16/M4 (5067-5109)
Cartridges - Selection valve
Capillary ST 0.17 mm x 700 mm SL/M (5067-5120)
In case of combining two Multi Cartridge Solutions:
Valve B / Port 3 - 2nd Selection Valve / IN Port
Valve B / Port 6 - 2nd Selection Valve / OUT Port
Installing the Valve Heads
Setting up the SPE Multi Cartridge Kit G4743A
Installing the Valve Heads
The valve drives are factory-installed in the Thermostatted Column Compartment, in
the Flexible Cube, and in the 1290 Infinity Valve Drive. The valve heads are
interchangeable and can be easily mounted.
At the first installation, the dummy valve has to be removed, see “Removing the Valve
Dummy” on page 15. The valve heads can be installed by mounting the valve heads
onto the valve drives and fastening the nut manually (do not use any tools).
Be sure that the guide pin snaps into the groove of the valve drive thread.
Removing the Valve Dummy
1 To remove the valve dummy, loosen the nut manually.
15
Installing the Valve Heads
Setting up the SPE Multi Cartridge Kit G4743A
Installing the Valve Head and Connecting Capillaries
For bio-inert modules use bio-inert parts only!
C AU T I O N
The valve actuator contains sensitive optical parts, which need to be protected from dust and
other pollutions. Pollution of these parts can impair the accurate selection of valve ports and
therefore bias measurement results.
➔ Always install a valve head for operation and storage. For protecting the actuator, a dummy
valve head can be used instead of a functional valve. Do not touch parts inside the actuator.
C AU T I O N
Column Damage or Bias Measurement Results
Switching the valve to a wrong position can damage the column or bias measurement results.
➔ Fit the lobe to the groove to make sure the valve is switched to the correct position.
C AU T I O N
Valve Damage
Using a low pressure valve on the high pressure side can damage the valve.
➔ When using multiple column compartments as part of a method development solution, make
sure that the high pressure valve head is connected to the autosampler and the low pressure
valve head is connected to the detector.
C AU T I O N
Sample degradation and contamination of the instrument
Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample
degradation and contamination.
➔ For bio-inert applications, always use dedicated bio-inert parts, which can be identified by
the bio-inert symbol or other markers described in this manual.
➔ Do not mix bio-inert and non-inert modules or parts in a bio-inert system.
16
NOTE
The tag reader reads the valve head properties from the valve head RFID tag during initialization of
the module. Valve properties will not be updated, if the valve head is replaced while the module is on.
Selection of valve port positions can fail, if the instrument does not know the properties of the
installed valve.
NOTE
To have the valve correctly recognized by the Flex Cube you must have the module powered off for at
least 10 s.
Installing the Valve Heads
Setting up the SPE Multi Cartridge Kit G4743A
1 The two different pins (blue & green) on the valve shaft must fit 2 It can be possible that you have to turn the valve head until the
into the corresponding grooves of the actuator axis. The
outside pin (red) must fit into the outside groove.
outside pin completely locks in. While turning the valve you
should feel some resistance from the drive.
Otherwise the pins on the valve shaft are not properly fitted into
the corresponding adapters on the valve drive.
17
Installing the Valve Heads
Setting up the SPE Multi Cartridge Kit G4743A
3 When the outer pin is locked into the groove, manually screw
4 Install all required capillary connections to the valve.
the nut onto the valve head.
NOTE
Fasten the nut manually. Do not use any tools.
5 Power on or power-cycle your module, so the valve head gets recognized during module initialization.
NOTE
Power Off the Infinity valve drive for at least 10 s.
18
Valve Head Parts Information
Replacement Parts
Valve Head Parts Information
Replacement Parts
Table 4
Replacement parts
Valve
Rotor
Seal
Stator
Head
Stator
Screws
Stator
Ring
5067-4145
Valve Head 2 Pos/10 Port valve head,
600 bar
0101-1415
(PEEK)
5068-0165
5068-0019
n.a.
5067-4146
Valve head 6 column selector, 600 bar
5068-0076
(PEEK)
5068-0077
5068-0089
n.a.
5067-4137
Valve Head 2 Pos / 6, 600 bar
0101-1409
(PEEK)
0101-1417
1535-4857
5068-0120
19
Valve Head Parts Information
Valve Head Parts
Valve Head Parts
NOTE
The figure below illustrates replacement parts for the valve heads, with the 12Pos/13Port Selector
valve as an example. The valves can vary in their appearance and do not necessarily include all of the
illustrated parts. Neither, every spare part is available for each flavor of the valve.
Figure 10
Valve Head Parts (example)
1
Stator screws
2
Stator head assembly
3
Stator ring screws (not available)
4
Stator ring (available for service only)
5
Rotor seal
6
Bearing ring
7
Spanner nut (available for service only)
*G4742-90101*
*G4742-90101*
G4742-90101
Part Number: G4742-90101
Edition: 07/2016
Printed in Germany
© Agilent Technologies, Inc 2013 - 2016
Agilent Technologies, Inc
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement