Use of Water Traps and Drying Filter with the FX

Use of Water Traps and Drying Filter with the FX
TECH REPORT
200:
Use of Water Traps
and Drying Filter
with the FX-5000™
Tension System
Document: Water Trap and Drying Filter Tech Report, Rev. 8.0
05-12-17
Culturing Cells in a Mechanically Active Environment™
Flexcell International Corporation  2730 Tucker Street, Suite 200  Burlington, NC 27215
800-728-3714  (919) 732-1591  FAX: (919) 732-5196  www.flexcellint.com
COPYRIGHT © 2009 FLEXCELL INTERNATIONAL CORPORATION
FLEXCELL® INTERNATIONAL CORPORATION
WATER TRAPS
PURPOSE
SPECIFICATIONS
The water trap provided with the FX-5000™
Tension System is used to remove moisture
from air that cycles through the FLEX IN
and FLEX OUT vacuum lines. On any given
cycle, the FX5K™ Tension FlexLink® may
draw air from the incubator through small
leaks in the BioFlex® baseplate gaskets. As
the incubator air is at a very high humidity,
this can result in moisture accumulation in
the FLEX IN and FLEX OUT tubing. The
water traps collect water and effectively dry
the air before it passes into the FlexLink®,
and subsequently into the valves and
transducers inside the equipment. They also
serve to collect any condensed water that
may have otherwise entered into the unit.
Fitting Size ¼” NPT (fittings
supplied)
Max Airflow Rate 53 CFM (1500
L/min)
Max Pressure 150 psi (1 MPa)
Max Temperature 125°F (51°C)
Collection Bulb 2.2 oz (65 mL)
Volume
Bulb Material Polycarbonate with
guard
Height 6.58 in (16.7 cm)
Width 1.97 in (5 cm)
Weight 1.25 lb (0.57 kg)
ASSEMBLY NOTES
The water traps come fully assembled with
an inline connector fitting on each side.
After the system is fully assembled, the
water traps will need to be connected inline
with the FLEX IN and FLEX OUT tubing
(Fig. 2) about halfway between the BioFlex®
baseplate and FlexLink®).
COMPONENTS
The water traps consist of an inline filter
with a collection bulb enclosed in a black
protective metal body (Fig. 1). The filter
removes moisture from the air and allows
dry air to pass through to the FlexLink®.
Removed moisture condenses and collects in
the bulb below.
Figure 2. Water trap in line with Flex In
tubing.
The water traps should be mounted to a solid
surface using screws or cable ties, in a
vertical position to assure that water will
drain to the bottom of the bulb. The water
traps should also be mounted in a position
Figure 1. Water trap
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lower than that of the FlexLink®. This will
force all condensed water to drain down into
the bulb. When mounting, leave enough
room underneath the bulb of each water trap
to allow water collection through the valve
at the bottom. The airflow direction required
for the water traps should also be taken into
consideration when mounting. Each water
trap will have two stickers on top indicating
which side should connect to the tubing that
leads to the baseplate (BPLT, see Fig. 3),
and which side should connect to the tubing
that leads to the FlexLink® (CTRL, see Fig.
3). Use the stickers as indicators when
connecting the trap inline. Before
connecting the water traps inline with the
FLEX IN and FLEX OUT tubing, set up the
entire FX-5000™ Tension system. Locate a
convenient position to mount the traps
according to the described conditions above.
Cut the FLEX IN and FLEX OUT tubing at
this point and connect the tubing to the two
side ports of each water trap. The water
traps are now ready for use with the FX5000™ Tension System.
MAINTENANCE NOTES
During normal use, some water may collect
in the bulb of the water traps. The amount of
water that collects in the water traps will
vary depending on how well the BioFlex®
baseplate and gaskets are sealed in the
incubator. An almost perfect seal will
produce minimal water in the water traps.
To improve the seal, vacuum or silicone
grease can be used on the gaskets, and a lead
weight and the Plexiglas® window provided
with the system can be used on top. Over
time, the gaskets will become more flexible,
and the seal will improve. The water traps
should be monitored hourly during initial
use of the system to determine how much
water accumulates over a given time period.
This will allow the user to predict how often
the traps should be emptied in future
experiments. Once the water traps are nearly
filled with water, the FX-5000™ Tension
system should be paused or stopped in order
to empty the water traps. Normal use with a
good baseplate seal will not require the stop
of experiments in order to empty the water
traps. To empty the water traps, place a cup
or other collection container underneath and
turn the valve at the bottom of the water
traps bulb. This will allow the water to drain
out. Once the water is drained, close the
valve on the bottom of the water traps.
REPLACEABLE ITEMS
The water traps provided with the FX5000™ Tension System should function
properly throughout the lifetime of the
system. Should any moisture be noticed
passing through the water traps and
condensing in the tubing on the FlexLink®
side of the water traps, a replacement filter
may be needed. Contact Flexcell® to order a
replacement filter.
Figure 3. Stickers on top of the water trap
indicate which side should connect to the
baseplate (BPLT) and FlexLink® controller
(CTRL).
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DRYING FILTER
PURPOSE
The drying filter is provided to remove
moisture from the FX5K™ Tension
FlexLink® System should any moisture have
bypassed the water trap. This filter is
necessary when water appears to have
reached the internal components of the
FlexLink® by way of the FLEX IN or FLEX
OUT tubing. The drying filter should also be
used on a regular basis to perform
preventative maintenance on the FX5K™
For
regular
Tension
FlexLink®.
maintenance,
Flexcell®
recommends
running the drying regimen after each
experiment with a 24 hour or longer
duration. The drying filter functions by
pulling air through desiccant beads and
subsequently through the FlexLink® valves
and transducer tubing. The desiccant beads
absorb the moisture out of the air, drying the
tubing and valves.
Figure 4. Drying filter
with fittings and tubing.
COMPONENTS
ASSEMBLY NOTES
The drying filter consists of a small glass
bulb in a plastic housing with desiccant
beads (Fig. 4). The drying filter comes fully
assembled with two fittings and lengths of
tubing to connect to the FLEX IN and FLEX
OUT ports of the FlexLink® unit. The
fittings include red locking clips to keep the
tubing connected.
To connect the drying filter to the FlexLink®
(Fig. 5), first remove the FLEX IN and
FLEX OUT tubing from the back of the
FlexLink®. Connect the blue polyethylene
¼” OD tubing from the drying filter to the
FLEX IN port on the back of the FlexLink®.
Connect the blue polyethylene 3/8” OD
tubing from the drying filter to the FLEX
OUT port on the back of the FlexLink®. The
drying filter is now ready for use with the
FX-5000™ Tension System.
SPECIFICATIONS
Fitting Size ¼” NPT female outlet with
male inlet (fittings supplied)
Max Pressure 90 psi (0.62 MPa)
Dimensions 3 ¾ in x 1 11/16 in diameter
(9.5 cm x 4.3 cm)
Dimensions 6 ¼ in x 1 11/16 in diameter
with fittings (15.9 cm x 4.3 cm)
Weight 0.4 lbs (0.18 kg)
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If it is believed that a significant amount of
water may have entered into the FlexLink®,
a longer drying regimen may be
programmed into the software. To do so,
simply modify the time in the default
regimen Drying. In many cases, if a large
amount of water has entered into the
FlexLink®, the unit will need to be shipped
to Flexcell® for repair.
MAINTENANCE NOTES:
REGENERATING SILICA DESICCANT
BEADS IN THE FX-5000™ DRYING
FILTER
After several drying regimen runs, the silica
desiccant beads inside the drying filter will
become saturated and turn from blue to pink
(Fig. 6). This change in color indicates that
the beads require regeneration to remove the
moisture. To regenerate the beads, they will
need to be removed from the drying filter
and heated in an oven.
Figure 5. Setup of a drying filter inline with
the FX-5000™ Tension System.
RUNNING THE FX-5000™ TENSION
SYSTEM WITH THE DRYING FILTER
CONNECTED
With the drying filter connected, open the
FX-5000™ software. In the Regimen menu
of the main window display, select Assign.
Choose the BioFlex Plate, no Loading
Stations platform assignment, the Shutdown
username, and the regimen entitled Drying.
Once the regimen is assigned, press Start.
Be sure that the vacuum source is turned
ON. The sound of air cycling through the
drying filter can be heard. Once the regimen
is complete, the drying filter should be
removed and replaced with the FLEX IN and
FLEX OUT tubing from the BioFlex®
baseplate. The drying regimen should have
the following parameters:
Shape
Min
Max
Freq.
DC%
Cycles
Duration
Figure 6. Drying filter ready for use (left) and
saturated drying filter (right).
To remove the desiccant beads:
1. Open the drying filter.
a. Use an 11/16” wrench to remove the
bottom tubing connector from the
drying filter. Use a 9/16” wrench to
hold the black fitting on the drying
filter in place while removing the
silver tubing fitting (Fig. 7).
½ sine
1.0
10.0
2.0
50.0
14400
0:02:00:00
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Figure 9. Remove top casing, rubber O-ring,
and fabric O-ring.
2. Dry the silica desiccant beads.
a. Pour out the desiccant beads into a
glass container.
b. Place the glass container in a drying
oven (Fig. 10a).
c. Optional: If a vacuum oven is
available, apply a vacuum up to -29
in Hg (-100 kPa; Fig. 10b).
Figure 7. Remove tubing connector.
b. Pinch the plastic housing on the two
marked locations and slide the
housing off the glass body (Fig. 8).
A
Pinch
Point
B
Figure 10. A) Saturated desiccant beads in
drying oven. B) Drying oven with vacuum set to
-29 in Hg.
Figure 8. Remove plastic housing.
c. Hold the drying filter upright and
unscrew the top black housing from
the glass body. Use a 9/16” wrench to
hold the bottom black fitting in
place.
d. Remove the top black housing,
rubber O-ring, and fabric O-ring
from the glass body (Fig. 9).
d. Set the temperature to 100-120 °C
and turn on the oven.
e. Leave the container in the oven for
1-2 hours, or until the beads have
turned blue (Fig. 11). With vacuum
applied, drying should only take 1530 minutes.
f. Turn off the oven. If vacuum was
applied, vent the oven to atmospheric
pressure.
g. Allow glass container to cool, but do
not wait too long or the desiccant
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4. Re-assembly drying filter (Fig. 13):
a. Place the fabric O-ring around the
central opening and rest it on top of
the desiccant beads.
b. Place the rubber O-ring on the
perimeter of the glass body.
c. Attach the top black housing and
tighten by hand. Use a 9/16” wrench
to hold the bottom black fitting in
place.
d. Slide the plastic housing around the
glass body and pinch to fit the tabs
under the top black housing.
e. Screw the bottom tubing fitting onto
the bottom black fitting. Use a 9/16”
wrench to hold the bottom black
fitting and a 11/16” wrench to
tighten the tubing fitting.
5. The drying filter is now ready for use
with the FX-5000™.
beads will absorb atmospheric
moisture.
Figure 11. Fully regenerated desiccant beads
in oven.
3. Return desiccant beads to the drying
filter.
a. If necessary, transfer the desiccant
beads to a smaller container to
facilitate pouring the beads back into
the drying filter.
b. When pouring the beads into the
filter, block the central opening to
prevent the beads from entering this
section (Fig. 12).
Cover here
REPLACEABLE ITEMS
The drying filter can be replaced at a
reasonable expense. Regular maintenance of
the FX-5000™ Tension System is important
to maintain longevity. Contact Flexcell® for
information on purchasing a replacement
drying filter.
Fill here
Figure 12. Cover center opening and fill
regenerated desiccant beads in outer chamber.
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Figure 13. Re-assembly of drying filter.
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