CPD 030 Critical Point Dryer and coating rate measuring device

CPD 030 Critical Point Dryer and coating rate measuring device
BAL-TEC AG
EM-TECHNOLOGY AND APPLICATION
CPD 030 GBE
1 CPDGBE.DOC
Operating Manual
CPD 030 Critical Point Dryer
and coating rate measuring device
Version 1.0
 BAL- TEC AG
Foehrenweg 16
PO Box 62
FL-9496 Balzers
++423 (0) 388 12 12
++423 (0) 388 12 60 (fax)
e - mail: [email protected]
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CPD 030 GBE
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Internet: http://www.bal-tec.com
Fundamentals .........................................................................................4
1
Introduction.................................................................................................5
1.1
Operating manual ........................................................................................5
1.1.1
Applicability range and system identification code.............................................. 5
1.1.2
Structure of operating manual........................................................................... 5
1.1.3
Target audience and mandatory reading ............................................................ 6
1.1.4
Access aids ................................................................................................... 7
1.2
Presentation convention ..............................................................................7
1.2.1
Warnings and notes on use.............................................................................. 7
1.2.2
Symbols and Abbreviations .............................................................................. 8
1.2.3
Position numbers and legends.......................................................................... 8
1.3
Customer documentation ............................................................................8
1.3.1
Scope of the documentation............................................................................. 8
1.3.2
Location of documentation ............................................................................... 8
2
Safety .......................................................................................................10
2.1
Safety concept of BAL-TEC AG.................................................................10
2.1.1
Purpose of use of the CPD 030 critical point dryer............................................ 11
2.1.2
Users and their responsibilities ....................................................................... 11
2.1.3
Operation modes........................................................................................... 12
2.2
Operational hazards and protective measures.........................................12
2.2.1
Operational hazards to persons ...................................................................... 12
2.2.2
Protective measures ...................................................................................... 14
2.2.3
General safety regulations.............................................................................. 14
3
Description and specification................................................................15
3.1
Introduction.................................................................................................15
3.2
Technical specification ..............................................................................16
3.3.
Description of equipment...........................................................................18
Normal operation .................................................................................26
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4.
Basic Operation......................................................................................27
4.1
Crtical Drying Method.................................................................................27
4.2
Basic Functions .........................................................................................28
4.2.1.
Cooling of the specimen pressure chamber...................................................... 28
4.2.2
Filling transition liquid into the specimen chamber........................................... 28
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4.2.3.
Filling up the Medium-Outlet Tube................................................................... 29
4.2.4.
Heating of Specimen Pressure Chamber ......................................................... 29
4.2.5.
Releasing Gaseous Transition Medium............................................................ 30
4.2.6.
Switching off unit........................................................................................... 31
5.
Performing a critical drying process .....................................................33
5.1
Preparation of Specimen ...........................................................................33
5.2.
Insertion of Specimen into Pressure Chamber .........................................34
5.3
Transfer the Specimen into Transition Liquid............................................34
5.4
Dry Specimens ..........................................................................................35
5.5.
Release Gaseous Transition Medium .......................................................36
5.6.
Remove Dried Specimen ..........................................................................37
5.7.
End Critical Point Drying Process .............................................................37
Special Operation.................................................................................39
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6
Unpacking, inspection and installation .................................................40
6.1
Unpacking ..................................................................................................40
6.2
Warranty ....................................................................................................41
6.3
Installation ..................................................................................................41
6.3.1.
Connection of CO2-Container ......................................................................... 41
6.3.2.
Connection of Medium Outlet ......................................................................... 42
6.3.3.
Connection of Gas-Outlet ............................................................................... 42
6.3.4.
Electrical Connection..................................................................................... 43
6.3.4
PREPARATION OF UNIT............................................................................... 44
7
CPD 030 Maintenance...........................................................................47
7.1.
Basic Maintenance.....................................................................................47
7.1.1.
Screw-on Cover of Specimen Pressure Chamber.............................................. 47
7.1.2.
Poral Filter in Medium-Inlet............................................................................. 47
7.1.3.
Poral Filter in Medium-Outlet .......................................................................... 47
7.1.4.
Pinhole Diaphragm in Medium-Inlet ................................................................. 48
7.1.5.
Pinhole Diaphragm in Medium-Outlet .............................................................. 48
7.1.6.
Checking Non-Return Valve ............................................................................ 48
7.1.8.
Exchanging of Heating Cartridges ................................................................... 50
7.1.9.
Exchange of Temperature Sensor ................................................................... 51
7.2.
TROUBLE SHOOTING..............................................................................52
8
Appendix..................................................................................................57
8.1
Spare parts, accessories and consumables ............................................57
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Fundamentals
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1
Introduction
1.1
Operating manual
CPD 030 GBE
1 CPDGBE.DOC
BAL-TEC AG in FL-9496 Balzers is the manufacturer of the
CPD 030 critical point dryer, which is used for critical point drying of biological specimens with minimal deformation of the
specimens.
This operating manual enables the user to operate the CPD 030
critical point dryer safely and in a correct manner.
1.1.1
Applicability range and system identification code
The description in this operating manual refer to the CPD 030 critical
point dryer with the f011owing device designation:
•
CPD 030 critical point dryer
In the event of questions or correspondence with BAL-TEC AG it is
helpful and accelerates the inquiry, if the user has the precise system identification code available, as specified on the identification
plate of the system. This information defines the system including
any customer-specific adaptations.
Note: For questions related to diagnosis (e.g. in case of faults), please
mention the detailed error message shown on the screen.
1.1.2
Structure of operating manual
The operating manual is intended primarily as reference. The information included is divided into four parts, according to tasks and topics:
Part 1 Fundamentals
The part “Fundamentals” gives the necessary fundamental knowledge which every user of the CPD 030 critical point dryer must have.
•
•
•
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Chapter 1
Chapter 2
Chapter 3
Introduction
Safety
Description and specification
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Part 2 Normal operation
The part “Normal operation” contains the information required for
regular operation of the CPD 030 critical point dryer. With this information the user can operate the system after it is ready for operation.
•
Chapter 4.
Basic Operation
•
Chapter 5.
Performing a critical drying process
Part 3 Special Operation
The part “Special operation” describes all work not performed during
normal operation such as installation, start-up of system, maintenance, trouble shooting and special functions.
•
•
Chapter 6
Chapter 7
Unpacking, inspection and installation
CPD 030 Maintenance
Part 4 Appendix
The “Appendix” contains general information, which always has to be
accessible to every user. This concerns information related to the
use of the operating manual (indexes) as well as data concerning the
CPD 030 critical point dryer itself (technical data).
•
1.1.3
Chapter 8
Appendix
Target audience and mandatory reading
The operating manual is intended for authorized users of the CPD
030 critical point dryer. Exclusively the customer determines who is
authorized as a user.
The requirements on the users vary with respect to the tasks to be
performed. We therefore distinguish different user roles. One user
may assume one or several of these user roles, if the user meets
the specified requirements.
Note: The definition of the user roles with the requirements on the corresponding roles can be found in section “Users and their responsibilities”.
Mandatory reading
Regardless of the user role assumed, every user is obligated to read
chapter “Safety”.
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1.1.4
EM-TECHNOLOGY AND APPLICATION
CPD 030 GBE
1 CPDGBE.DOC
Access aids
This operating manual facilitates fast access to desired information
by means of the f011owing access aids:
Table of contents
•
Index
•
Notes on left margin
•
1.2
Due to the task- and topic- based structure of the operating manual
complete information on a certain topic can be found easiest by
means of the table of contents.
Information on a certain task or a special topic can be found fastest
by means of the index in the appendix of this operating manual.
Within the chapters of the operating manual the notes on the left
margin of each page may be used for fast orientation.
Presentation convention
The operating manual contains abbreviations and marks to
designate text sections or notes. This section explains the
presentation conventions valid for the entire operating manual.
1.2.1
Warnings and notes on use
Please observe the significance of the f011owing warnings and
notes on use:
Warning: Hazard or unsafe handling which may result in injuries that
could be fatal.
Example: Danger of electric shock when in contact with a high tension line.
Caution: Danger or unsafe handling, which can cause only slight injuries but considerable damage to product, property or the environment
Example: Danger of early wear when running the engine with defective cylinder lubrication.
Note: Information on use which will help user to be able to technically use
the CPD 030 critical point dryer efficiently or understand the workings and
characteristics of the product.
Example: Unpack the instrument and check for exterior damage. Check
that the main unit is still in its blue shrink wrapping.
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1.2.2
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CPD 030 GBE
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Symbols and Abbreviations
In this operating manual the f011owing symbols and abbreviations
are used.
Symbols
Warnings and notes on use are marked by means of the above
symbols.
Abbreviations
Abbreviations are only used for dimension units.
1.2.3
Position numbers and legends
For precise identification of parts or elements the operating manual
includes several figures. Text items refer to the figures via position
numbers or legends.
Position numbers
Position numbers refer to the overview figures. They designate the
parts of the CPD 030 critical point dryer.
Legends
Legends are comprised of small letters and refer to items in the figures immediately above the corresponding text.
1.3
Customer documentation
Although the operating manual is an important document it is
merely a part of the customer documentation. This section
shows how the entire customer documentation is comprised.
1.3.1
Scope of the documentation
The entire documentation of the CPD 030 critical point dryer is comprised as f011ows:
•
•
1.3.2
Operating manual for CPD 030 critical point dryer
Documents (CE-certificate etc.)
Location of documentation
The operating manual is delivered as
•
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printed document on paper
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CPD 030 GBE
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The supplementary technical documents are delivered as
•
printed document on paper
Note: The EMC measurement protocol and further technical documents
are available from the manufacturer upon request.
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1 CPDGBE.DOC
Safety
Warning: The CPD 030 critical point dryer can be operated simply and
safely, if the system is operated according to the operating manual.
Hazards can however be caused by the system, if safety notes are not
observed.
2.1
Safety concept of BAL-TEC AG
The CPD 030 critical point dryer has only few operational hazards and can be operated simply and safely by every trained
user. Poorly instructed users however may cause damage and
may even incur injuries through improper use.
As a result of a hazard analysis a safety concept has been established which shall protect the user of the CPD 030 from hazards and
incorrect use.
The CPD 030 Critical Point Dryer has been developed in accordance
with VDE 0411 regulations concerning electronic measuring devices
and controls as well as the regulations of the elimination of radio interference f011owing VDE 0875.
The specimen pressure chamber has been tested by the Swiss
Federal Laboratories for Materials Testing and Research in Duebendorf at a pressure of 200 bar with 15.000 pressure alterations (EMPA
test report no. 183.723, dating from 21. Aug. 1985). In addition, safe
dimensions of the specimen pressure chamber have been confirmed by the Swiss Control Association for Pressure Installations for
an operating pressure of 150 bar (confirmation of 1. June 1983).
The specimen pressure chamber is secured with a bursting pane
against exceeding the permissible working pressure of 150 bar.
For safe operation it is absolutely necessary to set up and connect
the device according to the instuctions in section 6.3.
Besides the implementation of protective measures the core points
of this concept regulate authorization for use of the CPD 030 critical
point dryer and the responsibility of the individual users. Read the
summary of these points in the f011owing chapters.
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2.1.1
EM-TECHNOLOGY AND APPLICATION
CPD 030 GBE
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Purpose of use of the CPD 030 critical point dryer
The use in accordance with regulations of the CPD 030 critical point
dryer consists of critical point drying of biological or solid specimens
with minimal deformation of the specimens.
Typical areas of application are:
SEM specimens
•
Small biological or solid specimens for subsequent SEM analysis
Any other application without written approval of the manufacturer
shall constitute a violation. The manufacturer refuses to take any responsibility for damage caused thereby.
2.1.2
Users and their responsibilities
We designate all persons who work with or at the CPD 030 critical
point dryer as users. Because the requirements on the users vary
with respect to the individual tasks we distinguish different user
roles. The user may assume one or several of these user roles, if
s/he meets the specified requirements.
Customer
The “Customer” is the contractual partner of the manufacturer and is
authorized for legally binding signature of contracts.
The “Customer” procures the CPD 030 critical point dryer and is responsible that the system is used in accordance with regulations.
S/He determines, who is authorized to use the system.
Caution: Danger of wrong operation by poorly trained users. The customer is responsible that only users that have been instructed in detail and have been authorized (by the customer) operate the CPD 030
critical point dryer.
Operator
The “Operator” is an employee who has been trained to operate the
CPD 030 critical point dryer and is familiar with the critical point drying technique. S/He operates the system ready for use and can recognize and rectify errors.
The “Operator” is responsible that errors that he cannot rectify
him/herself, are immediately reported to the technician for rectification.
Caution: Danger of damage to the system. For any work that the operator is not familiar with or that is not described in detail in the operating manual, the operator is obligated to call the technician.
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Technician
The “Technician” is a professional with mechanical and/or electrotechnical basic training. He‘s able to install the CPD 030 critical point
dryer, connect it to the energy supply and start up the system. In addition, he performs maintenance and repairs on the system.
The “Technician” is responsible that the system is in a flawless state
for operation after installation, maintenance or repair.
2.1.3
Operation modes
The CPD 030 critical point dryer has the f011owing operation
modes:
Off
In the operation mode “off” the CPD 030 critical point dryer is
switched off and disconnected from the energy supply (mains) by
means of the main switch.
Normal operation
We designate as “normal operation” the modes in which the system
is either waiting in standby-mode or in normal operation.
Special operation
We refer to “special operation” when work deviating from the tasks in
“normal operation” is carried out on the system. This can be installation, maintenance or repair work.
2.2
Operational hazards and protective measures.
The manufacturer has minimized the consequence of existing
operational hazards by design and safety measures. However,
a user who is deficiently trained may cause damage or may
even be injured through improper use.
Pay special attention to all warnings and safety notes included in the
description of activities and the f011owing general safety regulations.
2.2.1
Operational hazards to persons
Observe the f011owing operational hazards and the possible countermeasures:
Warning: Danger of electric shock when touching parts under voltage. Disconnect the system from mains supply and disconnect the
mains connector before performing any work.
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2.2.2
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Protective measures
For protection from remaining hazards of the CPD 030 critical point
dryer different measures were implemented into the system design.
The most obvious measure is a completely closed casing.
2.2.3
General safety regulations
Generally, the f011owing safety regulations apply to the handling the
CPD 030 critical point dryer:
•
Every user is responsible for his own health.
•
Only instructed users, authorized by the customer are allowed to
work at or with the system.
For all activities at the CPD 030 the users must wear protective
clothing prescribed in the respective environment.
It is strictly prohibited to alter or remove protective equipment or
covers of any kind.
Every user must be trained with safe handling of the process gases
and fluids that are utilized in the CPD 30 unit.
•
•
•
•
•
•
•
•
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After every repair the user (technician) must verify a flawless state
of the system by a test run.
After repair full operation of the system can only be guaranteed, if
original spare parts according to the part lists are used.
Careful operation and preventive maintenance of the CPD 030 reduces maintenance costs and assures reliable operation.
By means of frequent checks and prompt rectification of even small
damages considerable damage can often be avoided.
Observation of this operating manual and all notes installed on the
system serves your own safety.
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3
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Description and specification
This chapter is directed toward all users of the CPD 030 critical
point dryer. The chapter shows the design, it describes the
function and designates the components of the system.
3.1
Introduction
Biological specimens intended for investigation in a scanning electron microscope must be dried before being introduced into the microscope (if the microscope does not have a cold stage) to allow the
specimen to be imaged. If specimens such as cell suspensions are
dried, tangential forces caused by molecular linkage affect the miniscule structures and cause deformation of the specimen.
Critical point drying with the CPD 030 is one method to prevent the
damaging effects of the above mentioned forces by bringing the liquid into the gas phase without freezing. The deforming forces can be
avoided by executing this process above the “critical point” of the
liquid where the phase boundary between gas and liquid phase no
longer exists.
This first section of the manual contains specifications for the CPD
030 critical point dryer. However familiar you may be with this type of
instrumentation, we suggest that you first inspect the instrument for
any signs of damage incurred during shipping (see section 6
Unpacking, inspection and installation).
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3.2
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Technical specification
Dimensions
Specimen chamber:
Usable volume
Ø 40 mm x 36 mm
Fluid filling
approx. 70 ml
Weight
approx. 33 kg
Connection data
Electrical:
Voltage
230 V / 240 V / 115 V
Frequency
50 / 60 Hz
Power consumption 220 VA
Main fuse F1 / F2
4 A slow blowing (230 V / 240 V)
5 A slow blowing (115 V)
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Transitional fluid:
Inlet
M 12 x 1.5
Outlet Ø 6 mm (R1/8")
Gas outlet
Ø 6 mm (R1/8")
Highest permissible pressure
Safety bursting membrane
approx. 150 bar
Refrigerator
Cooling capacity
85W
Refrigerant
R12
Quantity
120 g
Temperature measurement
COOLING range
adjustable
2°C to 12°C
HEATING range
adjustable
30°C to 45°C
Operating parameters
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Cool down time
approx. 2.0°C/min.
Heating time NORMAL
approx. 3.2°C/min.
Heating time SLOW
approx. 0.9°C/min.
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3.3.
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Description of equipment
The CPD 030 as shipped consists of the f011owing equipmen
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Housing of the unit with built-in specimen pressure chamber
-
Cooling unit for cooling of the specimen chamber
-
Continuously contr011ed heater for warming up the specimen
chamber
-
Temperature sensor with digital temperature display
-
Magnetic valves for media inlet as well as for the media and gas
outlet
-
Manually operated gas dosing valve for contr011ed gas outlet,
-
Non-return valve in the media inlet line
-
Pressure gauge for the pressure indication in the specimen
chamber
-
Safety burst membrane
-
Magnetic stirring device
-
Operating and display panel
-
Electrical part for the supply and control of the cooling unit, the
specimen chamber heater, the temperature measuring device,
the magnetic stirrer.
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- Set of accessories, including
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1
Connecting hose, BU 011 471-T for gas pressure bottle
2
Power cable without connector plug, 2 m, B 8010 054 87
3
Aperture disk DN 30 mm, BU 011 618
4
Stirrer, B 8010 114 76
5
Pin wrench 1/16, N 5701 144
6
"Lampenzieher", B 4683 981 Al
7
Poral Filter, BU 011 546-R
8
O-ring (two) for screw-on cover of specimen chamber, B
8010 114 75
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Operation and Display Panel (Overview)
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Brief operating instructions
2
Specimen pressure chamber
3
Operation and display panel
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Operation and Display Panel
1
Cooling
2
°C
Temperature display
3
Cooling
Indicator that cooling is activated
4
Heating
Key to activate heating
5
ber
Pressure
Pressure display in specimen cham-
6
Gas out
Valve is open when lit
7
Stirrer
Stirrer on, when lit
8
Stirrer
Key to activate stirrer
9
Display
Key for display checkout
10
Gas Out
11
12
Key to open gas outlet valve
Mains Switch
Medium Out Key to open MEDIUM OUT valve
13
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Key to activate cooling
Manually operated metering valve to
release gas
14
Medium In
Key to open MEDIUM IN valve
15
Medium Out Valve is open, when lit
16
Medium In
Valve is open, when lit
17
Heating
Heating is active when lit
18
Set Temp.
Key for nominal temp. Display
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Rear View
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1
Heating
Normal Speed or slow speed
2
Setpoint high Upper temperature set point
3
Medium Out Outlet for transition liquid
4
Outlet for gaseous transition medium
5
Cooling unit
6
Medium In
Inlet for transition liquid
7
CP 010
Control board
8
Set point low Lower temperature set point
9
Power connector
10
Fuses and mains switch
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Supply and Drain System
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Media inlet line
2
Magnetic valve for media inlet
3
Non-return valve
4
Burst membrane
5
Supply line to specimen pressure chamber
6
Pressure line to pressure gauge
7
Specimen pressure chamber
8
Outlet line of pressure chamber
9
Filter housing with poral filter
10
Magnetic valve for media outlet
11
Media outlet line
12
Dosing valve (manual) for gas outlet
13
Magnetic valve for gas outlet
14
Gas outlet line
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Specimen Pressure Chamber
1
Screw-on cover for specimen pressure chamber opening
2
View port to monitor liquid level
3
Pressure gauge
Specimen holders or specimen baskets (see section 9) can be inserted into the specimen pressure chamber. It can be closed with a
screw-on cover (1). The front sight glass (2) allows to observe the
level of the transition liquid as well as the achievement of the critical
point.
A cooling coil connected with the cooling unit and a heater built into
the specimen pressure chamber are designed to set the temperature necessary for the critical point drying process. The control is
carried out using a temperature sensor.
A magnetic stirrer system is built into the bottom of the specimen
pressure chamber to mix the liquids during the media exchange.
The magnetic stirring rod lies within the specimen chamber.
A burst membrane protects the specimen pressure chamber from
exceeding the permissible maximum pressure of approx. 150 bar.
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Cooling Unit
1
Dryer
2
Condenser, air cooled
3
Filler socket for cooling medium
4
Refrigerating compressor
Hermetically sealed cooling unit containing, a cooling compressor
(4) with a cooling power of 85 W and an air-cooled liquifier (2).
Cooling medium R 134a (CFC-free) filling amount 120 g.
The operating temperature of the specimen pressure chamber can
be set for a range between 2°C to 12°C. The maximum permissible
ambient temperature for a perfect function of the cooling unit is 30°
C.
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Normal operation
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4.
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Basic Operation
This chapter is directed toward users who operate the CPD
030 critical point dryer in normal (standard) operation.
As precondition for this work, the user must be familiar with the
chapter 3
Description and specification.
4.1
Crtical Drying Method
Drying water-containing biological specimens in air or under vacuum can drastically alter their structures or even destroy them
completely. They must therefore be dried by a gentler method. One
well-known method is "Critical Point Drying".
K= Critical Point
A= Initial state
E =Final state
T=Triple point
Å Air-drying / vacuum-
Phase diagram
The surface tension of the water in a specimen at the point at
which it changes from the liquid phase to the gaseous phase Å
can destroy a delicate specimen.
By increasing the pressure and temperature of the specimen it is
possible to dry it without crossing a phase boundary Ç. This is
possible because once the critical point has been passed, the density of the "liquid" and the density of the "gas" are the same. The
critical
point
for
water
is
228,5
bar
and
374°C. However, this high pressure and extreme temperature
would normally destroy a biological specimen. For this reason the
specimen must first be treated in a suitable transitional fluid such
as CO2 whose critical point of 73,8 bar and 31°C is considerably
more advantageous.
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4.2
Basic Functions
4.2.1.
Cooling of the specimen pressure chamber
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-
Carefully close specimen pressure chamber with screw-on
cover prior to operation of the unit.
-
Switch on the unit with main switch, located on the right hand
side of the middle mask of the unit.
-
Press key COOLING:
Cooling unit starts up. LED “COOLING” lights up.
Actual temperature of the specimen pressure chamber is shown
on the temperature display.
At a room temperature of 21°C the specimen pressure chamber is
cooled down to 10°C in about 7,5 minutes and subsequently falls
below this temperature to 3° to 4°C.
The cooling unit automatically keeps the temperature of the specimen pressure chamber below 10° C.
4.2.2
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Filling transition liquid into the specimen chamber
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If CO2 is used as the transition liquid, open the shut-off valve of the
CO2 gas pressure bottle.
-
Press key “MEDIUM IN”:
Medium inlet valve opens. LED “MEDIUM IN” valve lights up.
Manometer shows the pressure in the specimen pressure chamber (identical to the pressure in the CO2 gas pressure bottle).
-
Fill specimen pressure chamber up to the upper edge of the
front sight glass with liquid CO2.
-
Press key “MEDIUM IN” again:
Medium inlet valve shuts. LED of valve “MEDIUM IN” goes off. The
CO2-level in the specimen pressure chamber might rise a little.
4.2.3.
Filling up the Medium-Outlet Tube
-
Press key MEDIUM OUT:
Medium outlet valve opens. LED of valve "blind schematic" symbol
MEDIUM OUT lights up.
-
Allow the level of the liquid medium in the specimen pressure
chamber to drop to 2 to 3 mm below the upper edge of the front
sight glass.
-
Close medium outlet valve by pressing key MEDIUM OUT
again.
-
Press key COOLING again.
Cooling unit switches off. LED of the "blind schematic" symbol
COOLING goes off.
4.2.4.
Heating of Specimen Pressure Chamber
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Press key HEATING:
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Heater installed in the specimen pressure chamber switches on.
LED of the "blind schematic" symbol HEATING lights up.
The rising temperature of the specimen pressure chamber is
shown on temperature display.
With the rising temperature the pressure in the specimen pressure
chamber increases: display on manometer.
During the warmup of the transition liquid CO2, part of this liquid is transformed into gas. Due to that, the level of the liquid drops.
Having reached the critical temperature and the critical pressure
(31°C and 73,8 bar for CO2) the liquid CO2 is transformed into the
gaseous phase. The change of the physical state can be observed
through the front sight glass.
4.2.5.
Releasing Gaseous Transition Medium
Having reached the critical point of the transition liquid, the gaseous
medium is released from the specimen pressure chamber.
-
Check if manually operated gas dosing valve METERING
VALVE is closed.
Carefully turn the operating knob of the gas dosing valve clockwise to its
stop. If the valve is closed too tightly, the valve seat can be damaged.
-
Press key GAS OUT:
Magnetic valve for gas outlet opens. LED of "blind schematic" symbol GAS OUT lights up
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Open gas dosing valve METERING VALVE
-
Carefully turn operating knob anti-clockwise.
-
If a flow meter (recommended accessory) has been fitted (see section 1.5.), open gas dosing valve just enough to let the ball of the flow
meter float in the upper half of the measuring tube. Re-adjust gas dosing valve if the pressure decreases.
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Decrease of pressure in the specimen pressure chamber can be observed on the manometer. Atmospheric pressure should be reached in
about 10 minutes. After that the specimen pressure chamber can be
opened.
If the pressure drops too fast, the gaseous transition medium will condense due to the cooling caused by expansion. This will become visible
by the formation of fog in the specimen pressure chamber. In this case a
previously dried specimen can no longer be used.
-
After the gaseous transition medium has been released, switch
off HEATING and close medium outlet valve.
-
Press key GAS OUT:
Magnetic valve for the gas outlet closes. LED of valve "blind schematic" symbol GAS OUT goes off.
4.2.6.
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Switching off unit
-
Close shut-off valve of gas bottle.
-
Open screw-on cover of specimen pressure chamber.
-
Open medium inlet valve by pressing key MEDIUM IN: The gas
contained in the connection tube escapes through the open
specimen pressure chamber.
-
Close medium inlet valve by pressing again key MEDIUM IN.
-
Close specimen pressure chamber with srew-on cover.
-
Switch off main switch, located on the right hand side of the
middle mask of the unit.
-
Press key HEATING:
-
The heater is switched off.
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LED of "blind schematic" symbol HEATING goes of
-
Reduce pressure in gas connection tube from gas pressure
container to the unit to atmospheric pressure prior to switching
off the unit.
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Performing a critical drying process
This chapter is directed toward users who operate the CPD
030 critical point dryer in normal (standard) operation.
As precondition for this work, the user must be familiar with part 1
Fundamentals and chapter 4. Basic Operation.
5.1
Preparation of Specimen
-
Remove the specimen from the organ or the culture medium.
-
Wash in a physiological salt solution.
-
Fix chemically with a suitable fixing agent.
-
Wash out fixing agent with a suitable buffer solution.
-
Dehydrate the chemically fixed specimen with acetone or ethanol. If ethanol is used as a dehydration medium, which does not
mix with the transition liquid CO2, it must first be transformed
step by step into the intermedium amylacetate.
NOTE: The specimen to be critical point dried is prepared in the
same way as a sample for ultramicrotomy.
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Insertion of Specimen into Pressure Chamber
-
Transfer prepared specimen into the dehydration medium acetone, or possibly into intermedium amylacetate, in a suitable
specimen holder (see section ACCESSORIES).
-
Fill the specimen pressure chamber with the liquid (in which the
prepared specimen is already contained) to such a level that
the specimen is completely covered with liquid after insertion.
-
Quickly insert the specimen holder into the specimen pressure
chamber.
-
Tightly close the specimen pressure chamber with the
screw-on cover.
The prepared sample must always remain wetted until the critical point
drying process is carried out. Otherwise the structure of the sample would
be damaged after beginning to dry.
5.3
Transfer the Specimen into Transition Liquid
-
Switch on mains switch
-
Press key COOLING to pre-cool chamber
-
Wait until the pre-selected "lower" temperature has been
reached.
-
Open shut-off valve of the CO2 gas bottle
-
Fill the transition liquid into the specimen pressure chamber
-
Press key MEDIUM IN: medium inlet valve opens.
-
Fill specimen pressure chamber up to the upper edge of the
front sight glass.
-
Press key MEDIUM IN again: medium inlet valve shuts.
Mixing the two liquid media
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Press key STIRRER: magnetic stirrer is switched on. The
magnetic stirrer should not be used if the specimens are very
delicate.
Drain media mixture from specimen pressure chamber
-
Press key MEDIUM OUT: medium outlet valve opens.
-
Drain media mixture from specimen pressure chamber until the
specimen is just still covered by the liquid.
-
Press key MEDIUM OUT again: medium outlet valve closes.
-
Repeat procedure several times. Let the transition liquid in and
let the media mixture out as described above several times until
the liquid initially brought into the specimen pressure chamber
(acetone or amylacetate) has completely been replaced be the
transition liquid CO2. Experience suggests that this process
has to be repeated six to eight times.
-
Press key STIRRER to switch off the magnetic stirrer.
Fill specimen pressure chamber with transition medium
5.4
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Press key MEDIUM IN: medium inlet valve opens.
-
Fill specimen pressure chamber with transition medium CO2 to
a level approx. 2-3 mm below the upper edge of the front sight
glass.
-
Press key MEDIUM IN again: medium inlet valve closes.
Dry Specimens
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Check if all valves are closed.
-
LED's of "valve-blindschematic" symbols MEDIUM IN, MEDIUM
OUT and GAS OUT must be off.
Switch off cooling of the specimen pressure chamber
-
Press key COOLING: cooling unit switches off.
Warm up specimen pressure chamber.
-
Press key HEATING: heater of the specimen pressure chamber
is switched on.
-
Temperature and pressure in the specimen pressure chamber
rise: After reaching a temperature of approx. 20°C, the level of
the transition liquid will slowly begin to drop until the critical point
has been reached due to the transition of the liquid into the
gaseous state.
Reaching the Critical Point
5.5.
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The critical point of CO2 is reached at a temperature of 31°C
and a pressure of 73,8 bar: the specimen begins to dry.
-
Raise temperature and pressure slightly above the critical values prior to releasing the gas from the specimen pressure
chamber.
Release Gaseous Transition Medium
-
Check if the manually operated gas dosing valve METERING
VALVE is closed.
-
Press key GAS OUT to open magnetic outlet-valve.
-
Carefully turn operating knob anti-clockwise.
-
Watch flow meter (accessories): open gas dosing valve until
indicator ball floats in the upper half of the flow meter measuring
tube.
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Readjust gas dosing valve with decreasing of pressure in the
specimen pressure chamber.
-
Pressure decrease to atmospheric pressure should take approx. up to 10 minutes.
If the gas is released too quickly the gaseous transition medium
condensates (mist-formation). In this case the specimen gets wet
again and becomes unusable.
5.6.
Remove Dried Specimen
Switch off heater of the specimen pressure chamber
-
Press key HEATING: heater is switched off
Open specimen pressure chamber
-
After reaching atmospheric pressure the screw-on cover of the
specimen pressure chamber can be opened.
Close gas-outlet valve
-
Press key GAS OUT: magnetic outlet-valve closes.
-
Shut manually gas-dosing valve METERING VALVE by turning
the operating knob clockwise.
Remove specimen from specimen pressure chamber
5.7.
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Remove specimen holder with dried specimen from pressure
chamber for further processing.
-
Since the dried specimen is highly hygroscopic, it has to be
coated as quickly as possible with a thin metal- or carbon film
to protect it from atmospheric humidity.
-
If this is not possible, it is recommended to keep the specimen
in a desiccator until it is processed further.
End Critical Point Drying Process
-
Close shut-off valve of gas pressure bottle
-
Release pressure from gas connection hose
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Press key MEDIUM IN: inlet valve opens and gas escapes from
the connection hose through the open specimen pressure
chamber.
-
Press key MEDIUM IN again: inlet valve closes.
-
Switch off mains switch.
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Special Operation
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6
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Unpacking, inspection and installation
This chapter is directed to the technician who installs the
CPD 030 critical point dryer and performs the initial operational set-up.
The premise for this work is that the technician is familiar with the
chapter 2
Safety and 3 Description and specification
6.1
Unpacking
As soon as your critical point dryer and accessories have been delivered they should be checked for damage in transit.
1) Examine the exterior of the casing for evidence of rough handling.
2) Unpack the instrument and check for exterior damage.
3) Keep all packing materials for any evidence of miss-handling that
may be required or for possible future transport.
4) When removing packing check that there may be no rattling when
the unit is gently shaken..
5) Check all the items off against the delivery note and record the
serial numbers in the Appendix for future reference.
6) Report any evidence of damage both to the carriers and to BALTEC or our local agent immediately. We advise you that no responsibility can be accepted for damage in transit, if such a report is not
made within a period of 5 working days.
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Warranty
BAL-TEC cover all equipment made by BAL-TEC against defects in
materials and workmanship for one year from date of shipment.
BAL-TEC will not warrant performance of the equipment if accessories or consumables from other manufacturers are used.
BAL-TEC reserves the right to determine and will not allow a warranty claim for, defects which have been caused by the user's modification, misuse or other abnormal conditions of operation.
Static damage to internal components caused by inadequate handling during servicing or damage caused by using the incorrect
power is not covered.
BAL-TEC will repair or replace, at its discretion products which
prove to be defective during the warranty period provided that they
are returned, carriage pre-paid, to BAL-TEC.
No other warranty is expressed or implied. BAL-TEC is not liable for
consequential damage to equipment or material.
6.3
Installation
Required space for setting up the unit: width 490 mm, depth 660
mm.
To allow adequate intake of cooling air required for the refrigeration
unit and due to the inflexibility of the high pressure gas hose, the
wall distance has to be at least 150 mm.
6.3.1.
Connection of CO2-Container
For a correct implementation of critical point drying it is absolutely
necessary to use a pressurized gas container with a pipe (feedpipe), that the medium (CO2) can reach the specimen pressure
chamber in liquid form.
In order to prevent accidents the CO2-pressurized gas container
must be securely fastened in an upright position.
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- Insert seal DN10/6,5 x 2 (BU 011 646) in compression nut M 12 x
1,5 of high pressure gas hose (BU 011 873).
- Screw high pressure gas hose with compression nut M 12 x 1,5
onto connection MEDIUM IN.
- Connect other end of high pressure gas hose to gas pressure
container.
High pressure gas hose BU 011 873 and seal BU 011 646 are included in the accessory set of the unit.
6.3.2.
Connection of Medium Outlet
- Premounted hose nipple BU 016 448-T is already screwed onto
connection MEDIUM OUT.
- Mount PVC tube DN 5/10 mm onto hose nipple.
- Lead other end of tube into a sealable container preferably filled
with activated carbon.
Vapours of Acetone or Ethanol/Amylacetate used to dehydrate and
transfer the specimen into the transitional fluid respectively, must
not be allowed to freely diffuse into the workroom.
6.3.3.
Connection of Gas-Outlet
The use of a flow meter BU 011 102 -T (accessory) to control the
dosed gas outlet from the specimen pressure chamber is highly
recommended.
-
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Screw connecting tube of flow meter BU 011 1O2-T (accessory) onto outlet GAS OUT.
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Put flow meter on CPD 030 unit.
If no flow meter is available, control of dosed gas outlet can be
done via tube and water container:
6.3.4.
-
Premounted hose nipple BU 016 448-T is already screwed onto
connection GAS OUT.
-
Mount PVC tube DN 5/10 mm onto hose nipple.
-
Dip other end of tube into water-filled container: Exhaust of gas
can be contr011ed by the bubbles formed and regulated accordingly.
Electrical Connection
Check assigned connecting voltage for the unit of the mains connection and, if necessary, adjust required connecting voltage, observing, the f011owing procedure:
-
Push cover (2) by inserting e.g. a screw-driver into the upper
slot (3).
-
Remove voltage selector (4) and reinsert so that the desired
connecting voltage is shown at the display (1).
-
Pull out fuse holder (5) and insert fuses with the required value:
4 AT for 220 V respectively 5 AT for 115 V.
-
Reinsert fuse holder (5) and close cover (2).
Connecting the power cable:
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Fit locally required mains plug to power cable, considering the
f011owing figure of the power cable socket (L I + N + PE).
-
Connect power cable to wall socket.
6.3.4
PREPARATION OF UNIT
6.3.4.1.
Function check
6.3.4.2.
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-
Connect power cable
-
Switch on main switch, on the right hand side of the middle
mask.
-
Check Display Panel by pressing key DISPLAY CONTROL:
-
All 6 LED's of the "blind schematic" symbols must light up while
the button is being pressed.
-
Checking Magnetic Stirrer, by inserting magnetic stirrer
B
8010 114 76 (included in the set of accessories) into specimen
pressure chamber and pressing the key STIRRER:
-
LED STIRRER of "blind schematic" lights up and inserted
magnetic stirrer rotates.
-
Switch off stirrer by pressing key STIRRER again.
Setting "Upper" Nominal Temperature
Front View
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Rear view of unit
-
Press key SET TEMP
The pre-set "upper" nominal temperature appears on the temperature display while the key SET TEMP is being pressed.
-
Make adjustment with potentiometer HEATING located at the
rear panel of the unit.
"Upper” nominal temperature for CO2 as transition liquid is 40 C.
6.3.4.2.
Setting "Lower " Nominal Temperature
-
Press key COOLING
The cooling unit starts up, LED COOLING of "blind schematic"
lights up.
The actual temperature of the specimen pressure chamber is
shown on the temperature display
-
Press key SET TEMP
The "lower" nominal temperature is shown on the temperature display, while key SET TEMP is pressed.
"Lower" nominal temperature for CO2 as transition liquid at a pressure greater 50 bar in the gas bottle is 10 C.
-
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Make necessary adjustment with potentiometer COOLING
(lower) at the rear panel of the unit.
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Selecting Warm-up Speed
Switch is located on rear panel of unit
The warm-up speed of the specimen pressure chamber can be selected with switch HEATING at the rear panel of the unit:
-
Switch to position NORMAL: for normal sensitive specimens
(Increase of temperature approx. 3.2°C/min)
-
Switch position SLOW: for extreme sensitive specimens (Increase of temperature approx. 0.9°C/min)
Warm-up characteristics of the specimen pressure chamber:
1 Normal Speed
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2 Slow Speed
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CPD 030 Maintenance
This chapter is directed toward the technician who maintains
CPD 030 critical point dryer and performs repairs.
Note: As a precondition the technician must be familiar with part
Fundamentals and part Normal operation
Prior to opening the housing of the unit for maintenance work it is
crucial to close gas bottle, disconnect the gas line and disconnect
the mains cable.
If the housing of the unit has to be opened for maintenance work,
first remove the lower sight mask after having loosened the three
screws at the front of the bottom of the unit. Then the front plate
has to be pushed out of the spring clips from below.
7.1.
Basic Maintenance
7.1.1.
Screw-on Cover of Specimen Pressure Chamber
-
7.1.2.
Poral Filter in Medium-Inlet
-
7.1.3.
The poral filter (BU 011 546 -R) built into the medium inlet for
the transition liquid MEDIUM IN has to be cleaned periodically in
an ultrasonic bath. After closing the shut-off valve of the gas
pressure bottle and removing the connecting hose (hex-wrench
SW 17), the poral filter can be screwed out anti-clock wise with
the "Lampenzieher" (B 4683 981 A1) which is part of the
tool-set.
Poral Filter in Medium-Outlet
-
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Periodically check seal (B 8010 114 75) of screw-on cover and
replace if necessary.
The poral filter (BU 011 546-R) built into the filter housing (1) of
the outlet line of the specimen pressure chamber (see section
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7.7.) has to be cleaned periodically in an ultrasonic bath. After
loosening the screw joints (2) and (3) (hex-wrench SW 8 and
SW 12) remove filter housing from outlet line. After loosening
screw-joint (4) (hex-wrench SW 14 and SW 17), screw out poral filter from filter housing with the "Lampenzieher" mentioned
in section above.
7.1.4.
Pinhole Diaphragm in Medium-Inlet
-
7.1.5.
Pinhole Diaphragm in Medium-Outlet
-
7.1.6.
Clean pinhole diaphragm (BU 016 994) installed in inlet MEDIUM IN, if it has been plugged up with dirt. After removing the
connecting hose and the poral filter (see section 5.2.) screw out
pinhole diaphragm from medium inlet (screwdriver no. 4) and
clean it in ultrasonic bath.
Clean pinhole diaphragm (BU 015 541) installed in outlet MEDIUM OUT if it has been plugged up with dirt and is no longer
permeable for the liquid medium from the specimen pressure
chamber. After removing the connecting hose (hex-wrench SW
14) from medium outlet, remove pinhole diaphragm and clean it
in ultrasonic bath.
Checking Non-Return Valve
Periodically check whether there is no leak in the medium inlet- and
outletsystem for the specimen pressure chamber.
-
Cool specimen pressure chamber to 10° C
-
Fill with CO2 up to approx. 2 mm below the upper edge of the
front sight glass.
-
Warm up specimen pressure chamber
Level of liquid drops by a few mm due to physical reasons.
-
After reaching the critical point, cool specimen pressure chamber again to 10° C.
-
If there is no leak in the system, the liquid has to reach its original level again.
If this check shows that there is a leak in the system, it can be due
to a leaking non-return valve.
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In order to remove the non-return valve, proceed as f011ows:
-
Loosen screw joint (1) of medium inlet line with inlet valve (2)
(hexwrench SW 12).
-
Loosen fixing screws (3) of inlet valve (hex-wrench SW 8).
-
Loosen screw joint (4) between inlet valve and non-return valve
(5) (hexwrench SW 12).
-
Screw out non-return valve from connecting block (6)
(hex-wrench SW 14).
-
Open non-return valve by loosening screw joint (7 and 8)
(hex-wrench SW 12 and SW 14).
-
Remove valve piston from non-return valve and check for damage or contamination.
-
If the seal has been damaged, the complete non-return valve
(BU 016 546) has to be replaced.
-
Prior to refitting the new non-return valve, the two copper seals
(B 4119 513 -K) built into the connecting block (6) have to be
replaced.
-
Reassemble in reverse order.
Further possibilities for a leak in the system, see section xxx
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Exchanging of Heating Cartridges
If the preselected upper nominal temperature (40°C, if CO2 is being
used) is not reached, one of the two heating cartridges, built into
the specimen pressure chamber, can be defective.
-
Check by measuring ohm-value of heating cartridges (25 ohm)
or by examining for intact passage. For that, the connector plug
(1) placed in front of the specimen pressure chamber inside the
housing of the unit, has to be unpluged. The two heating cartridges are connected with the plug pins no. 6 and 7 and no. 5
and B respectively.
In order to replace a defective heating cartridge, proceed as
f011ows:
25.08.99
-
After loosening the two screws (3), remove the motor (2) of the
magnetic stirrer.
-
Remove the assembly plate (4) after loosening the three
screws (5).
-
Unscrew the three spacer elements (6).
-
Pull defective heating cartridge (7) out of the specimen pressure chamber.
-
Cut the connecting cable between defective heating cartridge
and connector plug.
-
Connect cable ends of connector plug and new heating cartridge (B 8010 055 27) with cable terminal.
-
Coat new heating cartridge with heat-conductive paste
(B 2753 420 -W) prior to inserting it.
-
Reassemble parts in reverse order.
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Exchange of Temperature Sensor
Failure of the temperature display may be due to a disruption within
the Pt 100 temperature sensor built into the specimen pressure
chamber or in its connecting cable.
-
Check by measuring the ohm-value (approx. 120 ohm at room
temperature) or by examining for intact passage. For that the
connector plug (section 5.7. Pos. 1) placed in front of the
specimen pressure chamber inside the housing of the unit has
to be unpluged. The Pt 100 temperature sensor is connected to
the plug pins no. 3 and 4.
To exchange the defective temperature sensor (section 5.7. Pos.
8) proceed as f011ows:
25.08.99
-
Remove motor (2) of magnetic stirrer and assembly plate (4)
-
Pull temperature sensor (8) out of the specimen pressure
chamber after loosening the cable screw joint (hex-wrench SW
8).
-
Cut connecting cable between defective temperature sensor
and connector plug.
-
Connect cable ends of connector plug and new temperature
sensor (B 8010 115 63) by soldering.
-
Coat temperature sensor with heat-conductive paste
2753 420-W) prior to inserting it.
-
Reassemble parts in reverse order.
(B
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7.2.
EM-TECHNOLOGY AND APPLICATION
TROUBLE SHOOTING
Problem
After switching on
mains switch, no
function on unit is
active.
After switching on
unit and pressing
key COOLING, the
cooling unit doesn't
start up.
After switching on
cooling unit temperature of specimen pressure
chamber doesn't
drop to pre-selected
lower nominal temperature or possibly
even rises slowly.
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Cause
Fuse F 1 and/or F 2
is,defective.
Remedy
Disconnect power
cable.
open cover of fuse
com
partment (see section
1.6.) and replace defective fuse.
Make sure correct
line voltage is selected!
Fuse values:
for 220 V 4 AT
for 115 V 5 AT
Fuse on power board Disconnect power
of slide-in unit CP
cable. Loosen screws
010 is defective.
of CP 010 slide-in
unit, pull it out of unit
and exchange fuse on
power board.
Fuse value:
for 220 V and 115 V
4 AT
Cooling unit overMake sure that room
heated due to a high temperature is below
room temperature
30°C.
and switched off.
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Problem
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Cause
Cooling unit overheated and switched
off, because of insufficient supply of cooling air.
Remedy
Make sure minimum
wall distance of 150
mm is maintained
and ventillation slots
on unit are not covered up.
Cooling unit is leaking coolant and does
not cooling effectively
anymore.
Contact BAL-TEC
supplier for repair of
cooling device (refrigerator).
Temperature display Temperature sensor Replace sensor and
remains at constant may be disconnected connection cable
value, despite of
or defective.
(see spares list)
COOLING or HEATING
Bursting pane, built Permissable presin for safety, breaks. sure of 150 bar has
been exceeded, because too much transition liquid has been
filled in and warmed
up.
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Open housing of unit.
Unscrew holder of
bursting pane and replace pane
(BU
011 980). Red side of
pane must be on the
outside.
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EM-TECHNOLOGY AND APPLICATION
Problem
MEDIUM IN valve is
open, but no transition liquid flows into
specimen preparation chamber
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Cause
Shut-off valve of gas
bottle is still closed.
Remedy
Open valve.
Gas bottle without a
standpipe (riser) has
been connected!
Use bottle with
standpipe, otherwise
only gaseous medium
can be obtained.
Diaphragm in MEDIUM IN line has
been clogged up.
F011ow procedure in
maintenance section
to exchange diaphragm
Temperature in
Temperature has to
specimen chamber is be below 10°C betoo high.
fore filling in the liquid
transition medium.
MEDIUM OUT valve Diaphragm in MEis open, but transi- DIUM OUT line has
tion liquid does not been clogged up.
flow out of specimen
preparation chamber
F011ow procedure in
maintenance section
to exchange diaphragm
GAS OUT valve is
open, but pressure
does not drop in
specimen preparation chamber
Open gas dosing
valve ccw as describe in section basic operation.
Manually operated
gas dosing valve is
closed.
Poral filter in outline is F011ow procedure in
clogged up.
maintenance section.
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EM-TECHNOLOGY AND APPLICATION
Problem
Level of transition
liquid recedes considerably (small
drop of level is normal), when specimen preparation
chamber is warmed
up.
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Cause
Remedy
Pipe connections are Verify that connecnot screwed tightly.
tions are tight. Check
leak tightness by applying soap water on
a brush to the connections.
Non return valve in
inlet line is not tight
due to a defective
seal or contamination.
F011ow procedure in
maintenance section
for repair.
Valve MEDIUM OUT
is not tight due to a
defective seal, damage on valve piston or
contamination on seal
surface.
Leak tightness can
be checked by connecting a hose to
MEDIUM OUT connection and submerging the other end of
the hose into a bucket
of water. Bubbles indicate a leak.
Refer to maintance
section for repair.
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© 1997 BAL-TEC AG
BAL-TEC AG
EM-TECHNOLOGY AND APPLICATION
Problem
Critical pressure is
not reached when
chamber is warmed
up.
Cause
Upper temperature
has not been
reached.
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Remedy
Make sure HEATING
is on, wait until temperature reaches
31°C.
If temperature does
not rise, check heater
cartridges (Maintenance section)
Verify that cover is
Screw-on cover is not screwed on tightly.
tight.
Check seal, change,
Seal on screw-on
if any damage can be
cover is defective.
observed.
Check seal, change if
Seal on sight glass is any damage can be
defective.
observed.
Non-return valve
leaks
MEDIUM OUT valve
leaks
F011ow procedure
specified above.
F011ow procedure
specified above.
F011ow procedure
GAS OUT valve leaks specified above.
Tube fittings are leak- F011ow procedure
ing.
specified above.
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EM-TECHNOLOGY AND APPLICATION
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8
Appendix
8.1
Spare parts, accessories and consumables
Specimen Container with 6 chambers 0 10 x 14 mm and
3 chambers 4 x 6 x 14 mm to accept up to 9 different
specimens. All specimen chambers can be closed with a
common cover of fine wire mesh. For suspensions, filter
paper can be used instead of the wire mesh.
To reduce the quantity of transition liquid used for the media exchange those specimen chambers that are not being used can be filled with Al-inserts 0 9,8 x 12 mm (BU
011 555).
Order No. BU 011 127-T
Sample basket
∅16 mm, height 12 mm, made of stainless steel, with
screw-on cover of fine wire mesh.
Order No. B 8010 170 37
Sample basket
as above, but ∅23 mm, height 12 mm
Order No. B 8010 170 38.
Sample basket
∅10 mm, height 21 mm, made of stainless steel, with
snap-on lid of fine wire mesh.
Order No. BU 011 126-T
Specimen transfer container
∅30 mm, height 32 mm, for transfering the specimens
into the specimen pressure chamber. With mechanism for
loading into the container. Made to receive up to 5 sample
baskets of typ BU 011 126-T.
Order No. BU 011 121 -T
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Specimen container
with insert for 8 cover glasses ∅18 mm. The insert can be
exchanged with one for 9 cover glasses ∅12 mm or for 7
cover glasses 22 x 22 mm. The cover allows the transfer
of the specimens in the dehydration liquid (e.g. aceton) in
spite of a fine wire mesh at the bottom of the container.
Order No. BU 011 128 -T
Insert for specimen container
for 9 cover glasses ∅12 mm,
Order No. BU 016 775 -T
for 8 cover glasses ∅18 mm,
Order No. BU 016 776 -T
for 7 cover glasses 22x22 mm,
Order No. BU 016 777 -T
Specimen holder
for 6 specimen grids ∅2,3 mm, suitable for drying cell
suspensions deposited on coated grids.
Order No. BU 011 122 -T
Specimen holder as above, but for specimen grids ∅3
mm. Order No. BU 011 123 -T
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Flow meter with connecting hose to the gas outlet GAS
OUT of the CPD 030 critical point dryer; for the
contr011ed outlet of the gaseous transition medium after
the critical point drying process Order No. BU 011 102 -T
Dosing valve complete
with mounting bracket and connecting parts for adding it
to the media inlet. For a contr011ed inlet of the transition
medium if extremely sensitive specimens have to be
critical-pointdryed.
Mounting of this accessory is possible without changes
of the unit. Order No. BU 011 129 -T
Pinhole diaphragm for media inlet
for screwing into the medium inlet line.
Opening of 150 um, Order No. BU 016 994 (already built
into basic unit)
Opening of 300 um, Order No. BU 016 995
Pinhole diaphragm for media outlet for inserting into medium outlet line.
Opening of 150 um, Order No. BU 015 541 (already built
into basic unit)
Opening of 300/um, Order No. BU 015 543
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1
1
1
1
1
1
1
1
EM-TECHNOLOGY AND APPLICATION
Description
Item
Order No.
Electrical part
Operating and display board
Cooling unit
Ventilator, 24 V DC
Specimen pressure chamber l.
Supply and drain line system
Pressure gauge, 160 bar
Seal Cu
1
2
3
4
5
6
7
8
BU B00 130 E/2
BU 005 072-T
B 8010 117 45
B 8010 119 14
BU 800 130 E/3
BU 800 130 E/5
B 8010 115 57
B 8010 115 58
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ELECTRICAL PART
1
1
1
1
1
2
2
2
2
1
25.08.99
Description
Item
Order nr.
CP 010 power board
Connection board
Ribbon cable, 20-pol.
Trafo, 165 VA
Filter, 4 A/250 V
Switch
Fuse, 5 x 20, 4 AT
Fuse holder, 5 x 20 mm
Fuse, 6,3 x 32,5 AT
Fuse holder, 6,3 x 32 mm
Mains cable, 1,5 m
11
12
13
14
15
16
17
18
19
20
21
BU 005 094 -T
BU 005 074 -T
BU 005 668 -T
B 8010 055 13
B 8010 055 14
B 8010 055 81
B 4666 448
B 8010 055 B0
B 4666 550
B 8010 055 78
B 8010 054 87
(for 220 V)
(for 220 V)
(for 115 V)
(for 115 V)
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SPECIMEN PRESSURE CHAMBER
1
1
1
1
1
I
2
1
1
1
25.08.99
Description
Item
Cover compl.
31
Sight glass, ∅40 x 20 mm
Seal
Support ring
Thrust washer
Resistor, PT 100
Heating cartridge
Motor, 24 V, 50 Hz
Magnet, 14-22-16
Stirrer rod, 5 x 20 mm
32
33
34
35
36
37
38
39
40
Order nr.
BU 800 130 EA
BU 015 435-X
BU 011 799
BU 011 798
BU 011 912
B 8010 115 63
B 801.0 055 27
B 8010 114 04
B 8010 114 66
B 8010 114 76
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1
QSG070GBE
CPDGBE.DOC
SCREW-ON COVER
Description
Item
Order nr.
Cover
Sealing ring holder
Sight glass, ∅34,5 x 20 mm
O-Ring, ∅28,3 x 1,7B mm
O-Ring, ∅37,82 x 1,78 mm
Thrust washer
Retaining ring
41
42
43
44
45
46
47
BU 011 413
BU 011 407
BU 015 434-X
B 8010 114 74
B 8010 114 75
BU 011 405
B 8010 115 4B
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EM-TECHNOLOGY AND APPLICATION
1
QSG070GBE
CPDGBE.DOC
 BAL- TEC AG
Foehrenweg 16
PO Box 62
FL-9496 Balzers
++423 388 1212 (tel)
++423 388 1260 (fax)
[email protected]
www.bal-tec.com
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© 1997 BAL-TEC AG
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