Glass Capillaries - World Precision Instruments
World Precision Instruments
Muscle
Physiology
2
Laboratory Equipment for the Life Sciences | 2013
Epithelial
Physiology
13
Microsdissecting
Microsurgery
21
Animal Surgery
& Support
34
Biosensing
53
Amplifiers,
Electrometers
71
Stimulators
92
Laboratory
Supplies
100
Glass, Holders,
Electrodes
114
Microforges,
Pullers
129
Micromanipulators
132
Microscopes,
Cameras
148
Pumps,
Microinjection
165
Spectroscopy
192
Index
209
New Research Tools from WPI
Muscle Physiology
Live Cell Imaging
The SI-CTS200 Cell Tester can be used for single cell or larger skinned
muscle fiber preparations. Translational
experiments from the single living cells
to the intact multi-cellular level can be
accomplished. The Cell Tester provides
researchers with the comprehensive
ability to investigate and characterize
the physiological, bio-mechanical and
bio-physical properties of single isolated living cells and extend these
findings to the sub- and multi-cellular level. New accessories like
the Biofluorometer and Optical Sarcomere Spacing system are
available for use with the Cell Tester. Page 3
This LCI incubator has a
unique, diffusion grid with air
input and return vents to
provide an air flow pattern for
consistent, even heating, with
no hot or cold spots in the
chamber. The external heater
can be placed far enough
from the system to eliminate
electrical and vibrational
interference from the heater. It has a high degree of temperature
precision and stability, minimal focal drift after equilibrium is achieved.
With accuracy of ±0.1ºC at the sample itself, and 0.2ºC across the
microscope stage, it allows for uniform heating of multiwell dishes.
Page 156
The new SI-H Horizontal
Tissue/Organ Bath system
(SI-HTB) combines the
ease of use and productivity of a traditional vertical
organ bath with the more
advantageous features of
single tissue physiology
platforms. The solid horizontal tissue bath design
is ideal for combination with electrophysiology on the same platform.
Intracellular measurements can share the stable solid base of the bath
system. WPI's new fiber-optic based, multi-channel biofluorometer
allow for tissue fluorescence measurements (calcium, NO, ROS) on the
SI-HTB platform. Page 5
Now, you can design a system to meet your needs and budget. And, it is
fully upgradeable in the future.
The new SI-BF100 is an LED-based fluorometer for life science applications. It is ideally suited for
ratiometric calcium detection
(FURA-2) and ATPase detection.
It also works well with voltagesensing dyes in neuroscience
and any other fluorometric
application. Page 6
The SI-H Compact Landgendorff needs no glass columns. It has both
Langendorff and working heart
perfusion modes. Page 7
The full-size SI-H Langendorff
system is designed as a perfusion
system for isolated, small
mammalian hearts. The system
includes the stainless steel sink, a
small reeling pump and specially
designed two-way Teflon taps.
A Neely system is also available.
Page 9
WPI offers a new modular design for all your favorite electronic
equipment. Install up to eight units in one convenient system. This
is perfect for use with the horizontal tissue baths, muscle testers
and cell testers. Units available include: SI-BAM21LCB amplifier,
SI-AOSUB anti-oscillation unit, SI-TCM2 temperature controller,
position controller for the
SI-CTS200, SI-SARCAM
Laser Sarcomere Spacing
system for the SI-MKB,
SI-COLUB Contant Load
unit, and the SI-MOTDB
linear motor controller for
the SI-MKB. Page 82
For short term or long
term studies of living cell
cultures under a microscope
or for time lapse video
research, a microscope
stagetop incubator is
necessary. Perfect for Live
Cell Imaging, STEV (the stagetop environmental control system) is a
compact environmental case that houses your culture wells and fits
on a microcope stage. This system offers precision control of both
temperature and carbon dioxide. CO2 is measured inside the stagetop
incubator, where it is most critical. Page 158
Pumps
The Nanoliter 2010 system is perfect for oocyte
injection and applications in the 2.3–69nL range.
It offers fast, accurate 2.3nL aliquot injections. It
works with glass pipettes. Nanoliter 2010 can be
combined with the Micro4 controller. Page 186
The reliable, compact MiniVacuum pump, sold with some SI-H Muscle
Testers, is also available separately. Maintenance
free • Minimal vibrations • Low noise • Long life
(185x72x72mm) Page 185
Microfluidics
The SF-MFP pump delivers
smooth, pulse-free flow of
volumes from 100nL to 90μL or
at rates between 250nL/min. to
10μL/min. Made of PEEK, this
tiny pump is compatible with
a range of solvents, solutions,
buffers and reagents. With this
pump, you can throw out your nut and ferrule connections. Now a
proprietary connection system uses powerful magnets for leak-proof,
self aligning connections that are a
snap to assemble. Page 191
Surgical Supplies
The 8L BioClave Mini comes preprogrammed for basic sterilization.
Choose either the 120ºC or 134ºC
temperature mode, and the cycle
time is fixed. This is the perfect
autoclave for small spaces. Page 32
Muscle Physiology . . . . . . . . . . . . . . . 2
Cell Tester l Vertical Tissue Bath
Compact Langendorff l Muscle Tester l Isolated Heart Systems
Epithelial Physiology . . . . . . . . . . . 13
Epithelial Voltohmmeter l TEER Measurement
l Ussing System l Perfusion System
Microdissecting, Microsurgery . . . 21
Scissors l Tweezers l Ear Punches l Vessel Clips l Mouse
Surgery Kit l Electrosurgical Unit l Cautery Instruments
Animal Surgery & Support . . . . . 34
EZ Systems l Analgesia l Stereotaxic l Monitoring
Blood Pressure Measurement lStereotaxic Systems
Biosensing . . . . . . . . . . . . . . . . . . . . . 53
Nitric Oxide Detection l Ion Selective Electrodes l Oxygen
Detection l pH meters
Amplifiers, Electrometers . . . . . . . 71
Intracellular l Extracellular l Transducer
Metal Microelectrodes l Voltage/Current Clamps.
Stimulators . . . . . . . . . . . . . . . . . . . . 92
Digital Stimulators l Multi-Channel l Linear Stimulus Isolator
l High Current Isolator
Check out WPI’s
Lab Glassware
—see page 112
Laboratory Supplies . . . . . . . . . . . 100
Adhesives l Cables & Connectors l Metal Wire l Luers &
Fittings l Laboratory Bases, Rods, and Clamps
Glass, Holders, Electrodes . . . . . . 114
Capillary Glass l Electrode Holders l Metal Microelectrodes
l MicroFil Non-metallic Syringe Needles
Microforges, Pullers . . . . . . . . . . . 129
Pullers l Bevelers l Microforges
Laboratory
Equipment
2013
Micromanipulators, Stands . . . . 132
Manual & Motorized Micropositioners l Magnetic Stands
l Tilting Base
Microscopes, Cameras . . . . . . . . . 148
Surgical, Stereo, and Inverted Microscopes l Cameras
l Illuminators l Heater Controller l Culture Dishes
Pumps, Microinjection . . . . . . . . 165
Peristaltic, Syringe, and Pneumatic Pumps l Microprocessor
Controlled Injector
Spectroscopy . . . . . . . . . . . . . . . . . 192
Spectrometers l Sample Cells l Light Sources l Fiber Optic Cables
l Fiber Optic Dipping Probe
Index . . . . . . . . . . . . . . . . . . . . . . . . . 209
Ordering Information, Terms & Conditions . . . . . . . . . . . . . . . . 216
M U SCLE PHYS IOLOGY
Equipment for
SINGLE CELL TO WHOLE ANIMAL
Muscle Research
Heidelberg
A World Precision Instruments Company
DAM50 Bioamplifier
DAM80 Bioamplifier
Non-Invasive Blood Pressure
SI-LANGC Compact
Langendorff with no
columns
SI-MT Muscle Tester
SI-LANG2
Landendorff
SI-MKB Muscle Tester
SI-MB Tissue Bath
SI-IPO Isolated
Perufused Organ
CS-BioTester Biaxial Tester
Signal Conditioning
Amplifier System
SI-CTS200 Cell Tester
2
SI-LANGWH
Working Heart
Duo773 Intracellular
Bioamplifier
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Refer to www.wpiinc.com/myocyte or request the new
Muscle Physiology catalog for details on the complete
line of muscle physiology equipment and accessories.
M U SCLE PHYS IOLOGY
Cell Tester
System
The SI-CTS system is a revolutionary new
research tool for studying single living cells. This
system, which is the result of blending the latest
technologies in electronics, mechanics and
optics, permits researchers to investigate living
systems at a new level using an innovative
experimental paradigm. The Cell Tester provides
researchers with the comprehensive ability to
investigate and characterize the physiological,
biomechanical and biophysical properties of
single isolated living cells, and to extend these
findings to both the sub-cellular and multicellular levels. The SI-CTS systems can be
used on single living cells, small multi-cellular
preparations or skinned muscle fibers and strips
without modification. It is designed to sit on the
stage of any standard, research-level, inverted
microscope while maintaining the optical path
of the microscope for simultaneous fluorescence
or confocal imaging.
The Cell Tester is an integrated system of
components needed to maintain and handle
single living cells, stimulate and perform
pertubations of the cells, and detect, amplify,
and record signals, like contractile force, from
the cells.
l Optical transducer for measuring force with
nN sensitivity and integral microtweezers for
facilitating cell attachment. Lifetime warranty on
optical heads.
l Equipped with a nanomotor for stretching and
relaxing cell with nm resolution and integral
microtweezers for handling cells
l A rotating cuvette system for easy alignment
of cells with the microtweezers increases
productivity. Interchangeable bath inserts
simplify the coating of cell mounting devices
and the handling of live cells.
l Signal conditioning amplifier system includes
a force transducer amplifier with multiple gains,
a position controller for moving the nanomotor
used to stretch and relax cells and to open
and close the microtweezers, a 2-channel
temperature controller, and an anti-oscillation
unit for eliminating the resonance frequency of
the transducer and its mounting support.
l Labtrax 8/16 data acquisition system
with MDAC software records force signals,
motor positions and other data through four
analog inputs. It is designed for controlling the
stimulator and the position of the linear motor
through two analog outputs and other devices
through two digital outputs.
l Includes non-cytotoxic bioadhesive
(MyoTak™) that facilitates bonding of cell to
measuring device
SI-CTS200 Complete Cell Tester System
System includes: Force transducer
and nanomotor with microtweezers,
rotating cuvette system, motorized
micromanipulators with pedestal bases,
signal conditioning system with transducer
amplifier, anti-oscillation module, position/
motor control module and temperature
control module, data acquisition system
with MDAC software for recording,
controlling stimulation and nanomotor
position, and MyoTak™ bioadhesive kit (5
week supply).
The rotating cuvette allows for precise positioning of the cells to be mounted, and it includes two
interchangeable inserts. The first holds any 35mm glass bottom dish for use when coating tweezers or
glass rods with MyoTak. Use the native cuvette insert for the live cells.
A single rat cardiac myocyte is held by MyoTAKcoated glass rods on the Cell Tester.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
3
M U SCLE PHYS IOLOGY
Muscle
Research
System
SI-MKB
The SI-MKB is the next research platform in the
progression of studying living systems from the cellular
level to whole organisms. It is the standard muscle
research system, which can be configured to study
skinned and intact muscle fibers, muscle strips and small
whole muscles. The modular design of the SI-MKB allows
it to be configured for turnkey solutions for specific
applications. The system is built on a solid platform
making precise mechanical and optical measurements
easy. Like the Cell Tester and the SI-MT systems, the
SI-MKB uses SI-KG optical force transducers and is
constructed with corrosion-free materials (stainless steel,
anodized aluminum or plastic).
Can be configured with specific components, like a linear motor or
a stimulator, for measuring:
l Responses to electrical stimulation, including tetany
l Mechanical properties of contracting and relaxing muscle strips
Supplied with a Labtrax 8/16 data acquisition system and MDAC
software for:
l Recording signals from the force transducer and the motor position
monitor, through four analog inputs
l Isotonic force and the effects of constant load
l Controlling the stimulator, or an external stimulus isolator, through an
analog output
l Twitch amplitude and kinetics, like contraction and relaxation times
and velocities
l Controlling the position of the linear motor through a second analog
output
l Slack, quick stretch-release, constant velocity and eccentric contraction
tests
l Controlling other devices through two digital outputs
l Afterloaded contractions from cardiac muscles
l Vibrational studies that simulate unloaded muscle shortening
l Quick temperature-change effects on skinned and intact muscle fibers
Can be equipped with components, like a laser diode or a
photometer, for measuring:
l Sarcomere length/spacing by laser diffraction at the same time muscle
force is measured
l Intracellular calcium concentration/distribution in intact muscle fibers
as muscle force is measured
l ATPase activity in skinned muscle fibers as muscle force is measured
SOME SYSTEMS AVAILABLE FOR INTACT MUSCLE FIBERS INCLUDE:
SI-MKBM-OXY
System with a 5cm heated cuvette and a linear motor
SI-MKB-OXY
System with a 5cm heated cuvette and a digital micrometer
Systems include: Base plate; force transducer and stand, digital micrometer or
linear motor (optional), micrometer or motor stand, cuvette and table, oxygenation
system, evacuation system, signal conditioning system with transducer amplifier
and temperature control module, anti-oscillation module and linear motor amplifier
(if applicable), Lab-Trax 8/16 data acquisition system with MDAC software for
recording force and position signals and for controlling stimulation and the
movement of the linear motor.
Tissue Mounts
Tweezer
Basket
Pointed
Hook
Blunt Hook
Spring Clip
Screw Clip
Tendon
Hook
●● Different styles (tweezers,
baskets, hooks, clips) for
mounting different types
of tissue to the transducer,
and to the linear motor or
micrometer
●● Most styles available in
sizes to fit each model of KG
transducer
●● Sold in a variety of sets,
each set contains two tissue
mounts that are appropriate
for the type of tissue being
tested
4
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Refer to www.wpiinc.com/myocyte or request the new
Muscle Physiology catalog for details on the complete
line of muscle physiology equipment and accessories.
Research system for higher throughput of
complex pharmacological/physiological assays
requirement up to 4-fold when compared with
standard 4-channel organ baths.
Versatile System
The SI-HTB combines advanced physiological
techniques with the throughput needed in
pharmacological assays in one flexible platform.
Upgrades to four or more channels are easy and
economical.
The motor option (SI-MOTTEST) turns
your system into a tissue work-out station
with isotonic, auxotonic and eccentric force
measurement capabilities. Nearly all established
myo-mechnical tests from stretch-release to
work-loops and muscle fatique are now possible
in a single organ bath system. Some of these
procedures require a length change solution
(software/hardware) like WPI’s MDAC package.
Future is Now
Multiple Bath Options
The new SI-H Horizontal Tissue/Organ Bath
system (SI-HTB) combines the ease of use and
productivity of a traditional vertical organ bath
with the more advantageous features of single
tissue physiology platforms.
The SI-HTB system breaks through the (large)
volume limitations of the traditional organ bath,
allowing volumes as low as 500µL in an inert,
teflon-based bath.
l Two channel system for increased
productivity, easily expanded to add
channels
The bath design allows multiple shape options
for thick, long, flat and thin tissue. When
pharmaceuticals are available in precious, small
amounts, you will appreciate this standard
feature. A wide range of transducers and tissue
mounting supports complement this freedom of
tissue shape, volume and size.
l Fully independent heating and fluid control
for each channel
l Low profile/small footprint
l Variable volume, chemically inert teflon bath
with shape configurations from variable to
fixed 500µL–10mL
Low Profile
l Modular, space-saving, blade-style electronics
(Control up to 4 channels with the electronics
in one chassis)
l Large variety of force transducers covering
mN–N forces, all with lifetime warranty
The low profile and small footprint of the
bath system, combined with the modular,
space-saving, chassis-mounted design of
the electronics, reduces the bench space
SI-HTB2
l Can be combined with automated fluid
control systems
The solid horizontal tissue bath design is ideal
for combination with electrophysiology on the
same platform. Intracellular measurements can
share the stable solid base of the bath system.
WPI’s new fiber-optic based, multi-channel
biofluorimeter, which is scheduled for release in
the fourth quarter of 2012, will allow for tissue
fluorescence measurements (calcium, NO, ROS)
on the SI-HTB platform.
Now, you can design a system to meet your
needs and budget. And, it is fully upgradeable
in the future.
Options
WPI’s 16-bit, high speed, Labview-based Muscle
Data Acquisition system SI-MDAC
WPI’s 16-bit, high speed, data acqusition with
industrial (CFR21 compliant) Notocord Data
recording and analysis software
Horizontal Tissue Bath, 2-Channel System
Includes:
2-Channel SI-HTB platform for isometric force (1)
SI-KGX Force Trandsucers (2)
SI-BAM21LCB Optical Force Transducer Amplifiers (2) SI-TCM2B 2-Channel Temperature Controller (1)
Signal Conditioning Amplifier System Chassis (1)
l Add a linear motor and controller (SIMOTTEST) to perform mechanical,
electrophysiological and optical techniques,
inculding isometric (standard), isotonic,
eccentric and auxotonic
SI-HTB4
l Add a photometer for Calcium, NOx, ROS
l Add an electrometer like the WPI Duo773 or
Electro705
Horizontal Tissue Bath, 4-Channel System
Includes:
2-Channel SI-HTB platform for isometric force (2)
SI-KGX Force Trandsucers (4)
SI-BAM21LCB Optical Force Transducer Amplifiers (4) SI-TCM2B 2-Channel Temperature Controller (2)
Signal Conditioning Amplifier System Chassis (1)
SI-HTB2M Horizontal Tissue Bath, 2-Channel Motorized System
l COMING SOON­—Add a 3-channel,
multi-purpose biofluorimeter for tissue
Fluorescence (calcium, NO)
Includes:
2-Channel SI-HTB platform for isometric force (1)
SI-KGX Force Trandsucers (2)
SI-BAM21LCB Optical Force Transducer Amplifiers (2) SI-TCM2B 2-Channel Temperature Controller (1)
SI-MOTTEST Linear motor (SI-MOT) with controllers (SI-MOTDB) (2)
Signal Conditioning Amplifier System Chassis (1)
MDAC Data Acquistion software (1)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
5
M U SCLE PHYS IOLOGY
Horizontal Tissue Bath
M U SCLE PHYS IOLOGY
t
Perfec
for:
tric
e
m
o
i
t
Ra um &
Calci ase
ATP
Biofluorometer
Now more reliable,
simplified and affordable
The new SI-BF100 is an LED-based fluorometer
for life science applications. It is ideally suited
for ratiometric calcium detection (FURA-2) and
ATPase detection (via NADH fluorescence). With
up to seven LED modules (wavelengths), the SIBF100 covers many fluorometric applications in
neuroscience and cell biology.
Recent advancements in optics and LED
technology simplify ratiometric calcium
imaging, making this equipment more
affordable. A breakthrough in WPI patented
technology allows the SI-BF100 to use
wavelengths below 380nm and produce
more light in those spectra. This technology
significantly cuts the cost of photometric
calcium imaging without sacrificing resolution
or quality.
How it Works
Up till now, calcium imaging systems have been
required to compensate for errors and noise
introduced by the complexity of their design.
The systems require mechanical filters and
use expensive xenon or mercury light sources.
The beauty of the SI-BF100 is its simplicity.
The elegance of its design reduces the noise
introduced into the system and the errors
inherent in traditional designs.
output to one or two photomultiplier inputs
on the front of the SI-BF100, which are
independently filtered for specific wavelengths.
This design allows you to monitor multiple
wavelengths from a single emission output.
The LED light source in the WPI design makes
this ratiometric fluorometer more compact,
energy efficient and affordable. As added
benefits, the low-power light source produces
much less heat, and it warms up in less than one
minute!
●● Two auto ranging photomultiplier inputs
allow you to monitor multiple wavelengths
from a single emission output that can be
comprised of any wavelength of light for
which an LED module is available
Monochromatic LED light sources using WPI
patented technology eliminate the need for
complex and expensive white
This incredible design is not limited to calcium
light sources and filter
imaging either. By simply replacing
wheels. Because
the 510nm emission
Unique fiber optic coupling probe allows
the LED modules
filters in front of the
for highly efficient transfer of light and
can be pulsed,
photomultipliers
ease of placement. Custom probes fit your
sampling
with
the desired
existing systems.
frequencies up
wavelength filters,
to 1,000 cycles per
your SI-BF100 becomes a
second are possible.
general purpose fluorometer for any application
you can imagine. Changing a filter involves
The LED light source emits specific excitation
removing the two screws that hold the filter
frequencies which travel through the probe.
carriage on the face of the SI-BF100, swapping
The excitation light can be comprised of any
the filter and reinstalling the integral SMA/filter
wavelength of light for which an LED module is
carriage.
available. The probe returns a single emission
●● Using a separate reference channel,
ultra-stable, continuous ratio calculations
automatically compensate for LED intensity
drift. This ensures less noise and produces
more accurate measurements.
BF-100Biofluorometer
Optional Components
M3301
Manual Manipulator for securing the probe
M10
Magnetic Base
●● LED light sources require less power, give
off less heat and are more compact and
affordable
●● Sampling rates up to 1kHz (1,000 ratios/
second maximum). At lower speeds, signal
averaging is used for noise reduction.
●● Application-specific probes are available for
existing tissue baths and cuvette systems.
SI-BF100 Specifications
●● Ratio noise is <0.05 peak to peak, drift is
less than 0.1 unit/hour
CALL for application
●● The warm up time of less than one minute is
a dramatic improvement over the common
20–60 minutes required by xenon or
mercury light sources
●● Replace the emission filter easily or change
the LED modules to transform the SI-BF100
into a general purpose fluorometer for many
other applications
6
Fiber Optic Light Input/Output . . . . . . . . . . .
Bandwidth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Ratio Noise . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Analog Output Range . . . . . . . . . . . . . . . . . . .
Analog Output Impedance . . . . . . . . . . . . . . .
Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Warm Up Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
SMA terminated
1000 ratios/second
< 0.1 peak to peak
0–10V (continuous, equivalent to a ratio 0–10)
100W
12VDC, 0.5A, (universal power supply, 110/240VAC)
<1 minute
3.5"H x 17"W x 13"D (88 x 431 x 330 mm)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Refer to www.wpiinc.com/myocyte or request the new
Muscle Physiology catalog for details on the complete
line of muscle physiology equipment and accessories.
Compact Langendorff
l Capability of measuring: left ventricular
pressure (LVP), perfusion or aortic
pressure, coronary flow, buffer
temperature, heart rate (HR), muscle
surface potentials, monophasic action
potentials (MAP), muscular tone and
force using optional force-displacement
method
l Port on aortic cannula for administering
drugs
l Optional capability of pacing/
stimulating the heart
l Optional capability of imaging calcium
distribution in a functioning heart
Patent Pending. U.S. and international
patent applications filed.
l In vitro studies of isolated hearts from small animals (mouse, rat, guinea pig, rabbit)
l Retrograde perfusion (Langendorff) or working heart (Janicki) technique
l Constant pressure or constant flow mode
l Occupies a small amount of bench space and no tall buffer columns
l Perfusion pressure electronically controlled at desired value between 0 and 500mmHg
l Highly stable environment for maintaining heart at desired temperature with
integrated temperature control system that can be set at 0.1°C increments
l Buffer reservoirs can be configured to form one large (2L), two medium (1L each) or
four small (500mL each) volumes of perfusion fluid
l Unique heart suspension module permits easy attachment of the heart to the system,
and the concise placement of the heart within the optional monophasic action
potential (MAP) sensor
COMPACT LANGENDORF SYSTEM
SI-LANGC
Compact Langendorf Working Heart System
System includes: Thermostatically controlled unit with built-in heaters (2), reservoirs, tap valves,
heart suspension cannula, heart chamber, oxygenation system, pressure transducers with cables (2),
2-channel pressure sensor amplifier, pressure controller, temperature controller, catheter balloons,
Peristar-Pro high output peristaltic pumps (2)
Optional Components
SI-SEN12AM4E-U
Manipulator with Removable Head (Unipolar)
SI-SEN12AM4E-B
Manipulator with Removable Head (Bipolar)
SI-SEN07RTH2
Buffer Temperature Sensor
SI-SEN13MAPMonopolar Action Potential Sensor
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
7
M U SCLE PHYS IOLOGY
Miniature system for isolated, perfused heart/ischemia reperfusion studies
M U SCLE PHYS IOLOGY
Langendorff
Isolated perfused heart system
Isolated
Organ
The SI-LANG1 and SI-LANG2 isolated
perfused heart systems were developed for
in vitro investigations of the hearts of small
animals according to the methods originated
by Oscar Langendorff. The system can be
configured easily to perform many of the
perfusion techniquesused to study isolated
hearts. Supplementary devices, like a special
MAP sensor or pacing/recording electrodes, can
easily be added to the system.
Features
●● Retrograde perfusion of isolated hearts
from mouse, rat, guinea pig, hamster
and rabbit
●● Measurements in constant pressure or
constant flow mode on the same device
●● Can be equipped with two columns for
using different buffers
●● Capable of recording aortic pressures as
high as 120mmHg
●● Equipped for measuring left ventricular
pressure (LVP)
●● Ports in the system are available for
measuring pressure, flow, temperature
and surface potentials, and for the
infusion or injection of drugs
●● Continuous filling of buffer columns
with use of a pump
●● Continuous oxygenation of buffers
●● Water-jacketed system for precise
temperature control
The Langendorff system is designed as
a perfusion system for isolated, small
mammalian hearts. Some special features
of this system include the stainless steel
sink, the small reeling pump and specially
designed two-way Teflon taps.
The Isolated Perfused Organ (IPO) system is
designed as a perfusion system for isolated,
small mammalian organs like the kidney, liver
or mesenteric bed.
LANGENDORF SYSTEMS
SI-LANG1
Langendorf Isolated Perfused Heart System, Single Buffer Column
SI-LANG2
Langendorf Isolated Perfused Heart System, Dual Buffer Column
Included: Table with base plate, shelf, sink • Console • Perfusion buffer column • Glass overflow tube
Teflon tap set • Heart suspension unit • Heart chamber • Spindle syringe • Pressure sensor holders
(2) • Oxygenation bubbler set • Oxygenation pressure equalizer set • Latex pressure balloons for left
ventricular pressure (LVP) catheter • PeriStar Pro 2-channel peristaltic pumps (2) • Circulating water
bath • Pressure transducers with cables (2)
Optional Components
SI-SEN12AM4E-U Manipulator with Removable Head (Unipolar)
SI-SEN12AM4E-B Manipulator with Removable Head (Bipolar)
SI-SEN07RTH2
Buffer Temperature Sensor
SI-SEN13MAPMonopolar Action Potential Sensor
8
The SI-IPOKIDNEY system is designed for
in vitro investigations of the perfused kidney
of small laboratory animals like mice, rats,
hamsters, guinea pigs and rabbits. An optional
perfusion chamber for liver and mesenteric
bed (gut) is also available.
During experiments, the artery of the organ is
cannulated and perfused with buffer solutions
using either constant pressure or constant flow
mode. Then, you can measure the changes in
the perfusion pressure. If desired, the effluents
can be collected for biochemical analysis.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Refer to www.wpiinc.com/myocyte or request the new
Muscle Physiology catalog for details on the complete
line of muscle physiology equipment and accessories.
Neely
Isolated working heart system
The SI-LANGWH isolated working heart system
was developed for in vitro measurements of
the mechanical activity and work performed
by hearts from small animals according to
the methods devised by J. R. Neely, et al.
This system is designed with a combination
of features from both the classic Langendorff
(retrograde perfusion) and Neely (working heart)
systems.
Features
●● Constant flow and constant pressure
modes
●● Can be equipped to measure perfusion
pressure, arterial flow and buffer
temperature
●● Perfusion pressure up to 120mmHg
●● Maintain buffer temperature with waterjacketed reservoir
●● Lubricant-free Teflon taps
Features
●● For use on small mammalian hearts
(mouse, rat, guinea pig, hamster and
rabbit)
●● External circulating water bath for the
buffer reservoir
●● Internal heater/controller for the organ
bath
●● Supplementary thermostat in the organ
bath
●● Working heart (Neely) and retrograde
perfusion (Langendorff) options in the
same device
●● Constant oxygenation of the buffer
●● Control of the left ventricular diastolic
pressure (LVDP), coronary flow and
cardiac output by variation of the left
atrial filling pressure and the hydrostatic
pressure in the aorta
Perfusion Chamber
Each perfusion chamber has an organ holder,
water bath, heater, vaporizer, perfusion inlet
and outlet, thermosensor port, supplementary
thermostat, mesh organ fastener, acrylic lid,
and blood vessels and ureter ports.
●● Measurement of the heart rate, atrial
and ventricular pressure and cardiac
output
Optional Accessories
●● Ports for drug administration
The following accessories may be added to
complete your system:
●● Pacing of the heart is possible
Temperature probe • Flow sensor and
monitor • Liver/gut perfusion chamber •
Data acquisition system
ISOLATED PERFUSED ORGAN SYSTEM
SI-IPOKIDNEY
Isolated Perfused Organ
(Kidney) System
Included: Heated tissue bath • Table with base
plate • Console • Perfusion buffer column •
Glass overflow tube • Pressure sensor holder •
Oxygenation bubbler set • Oxygenation pressure
equalizer set • Silicone tubing set, cables,
accessories • Peristaltic pumps (2) • Circulating
water bath • Pressure transducer with cable •
Pressure transducer amplifier
Optional Components
SI-LF-05-01-IPOAlternate Tissue Bath for
Liver or Mesenteric Bed
●● Special MAP sensor available
●● Tissue easily removed for biochemical
investigations
The Neely system is designed as a perfusion
system for isolated, small mammalian
hearts. Some special features of this system
include the stainless steel sink, the small
reeling pump and specially designed twoway Teflon taps.
NEELY SYSTEM
SI-LANGWH Langendorf Neely Perfused Working Heart System
System includes: Table with base plate, sink, shelf; console; buffer column; overflow tube; lung column;
elastic chambers (2); tap valve; heart suspension cannula; heart chamber; oxygenation system; pressure
transducers with cables (2); 2-channel pressure sensor amplifier; catheter balloons; Peristar-Pro high
output peristaltic pumps (2); circulating water bath; tubing, cables, and accessories.
Optional Components
SI-SEN12AM4E-U Manipulator with Removable Head (Unipolar)
SI-SEN12AM4E-B Manipulator with Removable Head (Bipolar)
SI-SEN07RTH2
Buffer Temperature Sensor
SI-SEN13MAPMonopolar Action Potential Sensor
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
9
M U SCLE PHYS IOLOGY
Perfused
System
BioTester
M U SCLE PHYS IOLOGY
Biaxial Test System for Biomaterials
The BioTester offers you a complete system for testing small biological specimens and
analyzing the test results. Skin, ligaments, blood vessels, heart valves, sclera, membranes,
scaffolds– BioTester can examine any planar biological or replacement tissue. Easy to use, yet
powerful image tracking and analysis software delivers synchronized data, image and video
management. Data can be exported to spreadsheets or analysis software.
l Quick and easy sample mounting of test samples as small as 3mm x 3mm
l High Resolution (integrated) CCD camera provides synchronized video tracking for live
images (up to 15 frames/sec) and real-time analysis
l Image tracking and analysis software included.
lPrecision measurement of small samples (3mm-15mm square)
l Uniaxial or biaxial tension tests of planar tissues
l
Multi-modal cyclic, simple and relaxation testing
l Simple USB interface offers a quick connect to a control computer
with the included image and marker tracking software
l User-friendly, comprehensive software package offers live data
graphing and imaging to confirm the quality of the data
collected
Sample Mounting
The unique tungsten BioRakes (shown at right) easily pierce the toughest
and most delicate soft tissue samples and provide distributed attachment
sites across the geometry of the sample for uniform attachment and
deformation across the edge of the sample. The sharp rakes will not
damage fragile samples.
Software
The software features a user-friendly interface with live data graphing and
imaging. The menu-driven setup offers a virtually limitless number of test
stages and duration combinations. Previously used tests and templates
can be easily edited for quick test designs. Live test status and data
graphing provides real-time data monitoring. Analysis data can be easily
exported to spreadsheets or other scientific modeling software.
Image analysis software is provided with the BioTester to deliver
BioTester Specifications
Force Capacity.........................................500, 100, 2500, 5000 mN, 10 N, 23 N
MAX. ELONGATION RATE....................................................................................10 mm/s
MAX. STRAIN RATE (5mm specimen)................................................................. 200%/s
Force Accuracy........................................................................0.2% of force capacity
Spatial Resolution (Actuator).................................................................. >0.1 µm
Spatial Accuracy (Actuator)...........................................................................10 µm
Spatial Resolution (Image Analysis)......................................................... 1⁄8 pixel
synchronized data, image and video management. Image features
are easily tracked and synchronized to measured data and export to
spreadsheets or other scientific analysis software. Images are available as
JPG or AVI files for presentation and import into other image processing
applications.
10
MAX. Force Data Rate......................................................................................... 100 Hz
Image Rate...........................................................................................1280x960 - 15 Hz
CS-BIOTESTER
Biaxial Biomaterial Tester
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
MicroSquisher
Micro-Scale Compression System
Cell Aggregate Compression
role in the organization of cells within aggregates.
These properties can be determined by analyzing
the force-time curve and test images from a parallel
plate compression test.
glass plate
The
MicroSquisher
is designed to perform compression
testing on specimens between 50 µm and 2 mm
with force resolutions as small as 50 nN. Forces
are determined from the deflection of a flexible
cantilever beam to which one compression plate
is attached. Displacement control is achieved
by manipulating the base of that beam using a
motorized piezo stage.
The specimen can be tested in ambient air or in a
temperature-controlled fluid bath. An integrated
camera system allows synchronized imaging at up
to 5Hz.
MICROSQUISHER Specifications
Specimen Size...................................... 50–2000 µm
Force Resolution.................................... <0.05 µN
Sampling Rate........................................... Up to 5 Hz
cell aggregate
(250µm diameter)
glass plate
Micro-Scale Material
Characterization
The MicroSquisher can be used to determine the
compressive stress-strain properties of a variety
of materials (hydrogel microspheres, small tissue
samples, scaffolds and cell
aggregates) with peak forces
ranging from 1μN to 1mN.
The MicroSquisher image analysis module
quantifies the aggregate profile, allowing cellcell and cell-medium interface tensions to be
calculated.
The device can perform
displacement-controlled
compression and stress
relaxation testing.
CS-MICROSQUISHER
Micro-Scale Compression System
MechanoCulture
Mechanotransduction cell culture system
l Uniaxial, equibiaxial, or nonequibiaxial strains
l 0 - 15% strain
l User downloaded test protocol
l Monolayer cells on substrate or 3D
constructs
l Autoclavable
l Affordable and expandable system
l VHS videocassette-sized units fit
easily in standard incubator
Easy to use software
mechanoculture
Specifications
Sample Size . . . . . . . . . . . . . . . . . 35mm in diameter
Maximum Frequency . . . . . . . . . . . . . . . . . . . . . . 2Hz
Maximum Strain . . . . . . . . . . . . . . . . . . . . . . . . . 15%
Maximum Sample Thickness . . . . . . . . . . . . . . . 2mm
X-Y Strain Ratios . . . . . . . . . . . . . . . . . . 1:0, 1:1, 2:1
MechanoCulture allows researchers to culture
cells on a deformable substrate or as part of
a 3D matrix to understand how mechanical
environment affects cell differentiation, mitosis
and signalling.
CS-MECHANO-SYS
The MechanoCulture software is used to
specify test parameters. The test sequence can
then be downloaded to the instrument. A run/
pause button is used to initiate, pause and stop
the test. An LED display indicates the state
of the unit, including the number of cycles
remaining in the original protocol. The base
unit can be stopped and disconnected from its
power source without losing track of its position
in the protocol.
Strainable Substrate for Culturing Cells
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
11
M U SCLE PHYS IOLOGY
The interface tensions that exist play an important
Uniaxial Tissue Tester
lQuality testing
M U SCLE PHYS IOLOGY
lCost-effective
lEasy to use
lMultiple attachment options
lAvailable imaging and temperature controlled fluid bath
Specifications
Test specimen as small as 3x3mm and as
large as 20x100mm. Elongation rates
can be as high as 50mm/s
and applied forces
can be monitored at
100Hz. Load control is
also possible, and simple
or complex protocols can be
specified. A variety of load cells are
available, with force resolutions as low as
1mN and capacities as high as 100N.
Test protocols can be saved and modified. Realtime force and displacement graphing facilitates
test validation.
Software
The CS-UStretch makes it possible to carry
out dependable, mechanical testing of soft
materials for a reasonable price.
Applications
Typical applications include hydrogels, skin,
muscle, blood vessels, heart valves, ligaments,
sclera and scaffolds.
Optional image tracking and analysis software
allows images to be analyzed to identify and
quantify strain non-uniformities and regional
property variations (like those in the specimen
at right). Integrated software makes it easy to
specify test protocols, both simple and complex.
Easy to use software lets you visualize
results in a variety of ways.
Mount a specimen quickly and precisely using the
patented BioRake attachment system.
CS-USTRETCH Specifications
Specimen Size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3x3mm up to 20x100mm
Force Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.5N, 1N, 2.5N, 4.5N, 8.9N, 22N, 44N, 110N
Force Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.2% of force capacity
Maximum Elongation Rate . . . . . . . . . . . . . . . . . . . . . . . . . . 50mm/s
Attachment Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . BioRake and Clamp Sample Mounting System
Camera, Tracking and Analysis Software (optional) . . . . . . Full motion and strain analysis
Heated Bath (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . User controlled up to 40˚C
Report Generating Software . . . . . . . . . . . . . . . . . . . . . . . . . . .avi WYSIWYG and Excel
Image Rate and Resolution . . . . . . . . . . . . . . . . . . . . . . . . . . 15Hz, 1280 x 960
Maximum Force Displacement Rate . . . . . . . . . . . . . . . . . . . 100Hz
12
Design and save your test protocols. Then, use the
real-time force/displacement graphs to validate
your tests.
CS-USTRETCH Uniaxial Biomaterial Tester
Check the WPI website for complete
details and specifications.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Trans Epithelial Electric Resistance (TEER) Measurements
During the last two decades TEER measurements have
become universally established as the most convenient,
reliable and non-destructive method to evaluate and
monitor the growth of epithelial tissue cultures in vitro.
The confluence of the cellular monolayer is quickly
determined by a sharp increase in TEER. Recently there
has been a significant surge of interest in introducing a
combined electrode for resistance measurements in the
Millipore 96-well PAMPA (parallel artificial membrane
permeability assay) plate.
TEER measurement technology, which was first
introduced by WPI in the mid-1980’s, has since been
perfected and expanded to include a range of TEER
related manual and automatic instrumentation.
Epithelial Voltohmmeter
● Manual TEER measurements of
epithelial cells in 6-, 12-, and
24-well plates
● Electrically isolated meter that
plugs into a standard outlet for
continual readout without push
buttons
● Compatible with Endohm
chambers
● STX2 manual electrodes and test
electrode included with every
meter
The EVOM was the first instrument designed specifically to perform
routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue
culture research. EVOM2 is the next generation, redesigned for ease of
use. The EVOM2 not only qualitatively measures cell monolayer health,
but also quantitatively measures cellular confluence. The unique electronic circuit of the EVOM2 and the included STX2 electrode detect the
confluence of the cellular monolayer. When
combined with WPI’s Endohm chamber, the
EVOM2 can also be used to perform more
accurate quantitative measurements or lower
resistance measurements like trans endothelial
electrical resistance measurements.
The isolated power source of the EVOM2 was
specifically designed to avoid adverse effects
on tissue and the formation of electrode metal
deposits, even when it is plugged into a standard wall outlet. Now, the EVOM2 is always
on when you need it. In addition, its rechargeable battery allows up to 10 hours of mobile
use. The four and a half digit readout provides
a range of 1-9,999 Ω. The included test electrode lets you calibrate the
resistance measurements for an accurate reading every time, and the voltage meter never needs calibration. An analog BNC output is standard with
the EVOM2, providing an output port for recording data or remote display
of the EVOM2 output.
EVOM2 comes complete with the popular STX2 “chopstick” electrodes,
4 mm wide and 1mm thick. Each stick of the electrode pair contains a
silver/silver-chloride pellet for measuring voltage and a silver electrode for
passing current. The small size of each electrode is designed to facilitate
placement of the electrodes into a variety of standard cell culture wells.
EVOM2 SPECIFICATIONS
Membrane Voltage Range
±200 mV
RESOLUTION
0.1 mV
Resistance Range
0 to 9999 Ω
Resistance Resolution 1 Ω
STX2
STX3
EVOM2 Epithelial Tissue Voltohmmeter (includes STX2 electrode set)
REPLACEMENTS AND ACCESSORIES
STX2
Replacement “Chopstick” Electrode Set
STX3
Adjustable Tip Spread “Chopstick” Electrode Set
3993
Electrode Adapter (for electrodes with 2 mm pins)
91736 Replacement Battery, Rechargeable NiMH
91750 EVOM2 Test Resistor
AC Square Wave Current
±10 µA nominal at 12.5 Hz
Power
Internal rechargeable 6V NiMH 2200 mAH battery with external 12 VDC supply for recharging
Nominal Battery Run Time
10 hours
BNC Output
1-10 V (1 mV/ohm)
Dimensions
19 x 11 x 6 cm (7.25” x 4.25” x 2.30”)
Weight
1.4 kg (3 lb)
Electrode Connection
RJ-11connector (telephone style)
Test ResistorExternal
Environmental Range
10-38°C (50-100°F )
0-90% non-condensing relative humidity
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
13
EPITH ELIAL PHYS IOLOGY
EVOM
2™
TEER measurements in High Throughput
STX100
EPITH ELIAL PHYS IOLOGY
Series Electrodes
● Designed for 24-well HTS plate (Corning Costar and BD
Falcon) and with 96-well plates (Millipore and BD Falcon)
● Improved accuracy down to 5 Ohm
● Sterilized with EtO, alcohol or bactericide
With the development of a High Throughput Screening (HTS) protocol for
faster drug discovery, a new line of cell culture filter plates have been
introduced by several major cell culture insert manufacturers. These
HTS plates normally have either 24 or 96 individual cell culture inserts
“bonded” together as one plate so that it can be handled by a robot
apparatus. In response to these developments, WPI has developed an
automatic REMS system and a manual electrode, STX100, for TEER
measurements using HTS plates.
STX100’s design is based on the same reliable design principle
as the universally used STX2 electrode, with several important
modifications. The size of the electrode tip has been reduced to 1.5 mm
to facilitate positioning through the narrower slit of the HTS plate. The
STX100 electrode itself is constructed using a stronger material for higher
durability and maximum usage applications. The bottom section of the
electrode is shaped to fit neatly into the “keyhole” shaped filter well.
This enables the STX100 electrode to produce increased accuracy and
reproducibility of TEER readings (±5Ω) compared to the standard STX2.
Several versions of STX100 are available, designed to fit the Corning
Costar 24-well HTS plate, the Falcon 24 well HTS plate, and the Millipore
Multiscreen CaCo 96-well plate. Measurement can be directly performed
when the HTS plate is in either a common or divided tray, reducing
the possibility of contamination as well as mechanical damage to the
cultured cells.
STX100C STX100 for Corning Costar HTS Transwell-24
STX100F STX100 for BD Falcon HTS Multiwell Insert System
STX100M STX100 for Millipore MultiscreenTM HTS 96-Well Plate
STX100C96 STX100 for Corning HTS 96-Well Plate
OPTIONAL
13685
13347
2851
500184
ACCESSORIES
Modular Cable, 7 ft
Chart Recorder Adapter
Standard BNC Cable, 5’2”
Standard BNC Cable, 10 ft (3m)
CaliCell™
Cell culture cups with synthetic membrane for testing
STX electrodes, Endohm and Ussing chambers
It takes a long time and a lot of work to grow a batch of cells, so you will want to make
certain that your test ap­pa­ra­tus is func­tion­ing properly. The CaliCellTM provides a quick and
positive way to test STX electrodes, EVOMs, Endohm, and Ussing chamber.
The CaliCellTM is a major improvement in TEER electrode calibration. Its membrane makes
use of our unique electric current constriction technology to produce resistance readings
comparable to those obtained with real cell cultures. The CaliCellTM does not have to be
refrigerated, and can be cleaned and sterilized with alcohol. Readings will not drift over time
as long as the unit is kept in good physical condition.
CALICELL-12
CALICELL-24
14
12 mm Calibration Cell for Endohm-6/Endohm-12
24 mm Calibration Cell for Endohm-24
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Screening (HTS) cell culture filter plates
EPITH ELIAL PHYS IOLOGY
Also for use with Millicell ERS-2
Endohm™
For TEER measurement of endothelial cell cultures in individual cups
● Compatible with EVOM2
● Improved accuracy of 1-2 Ohm
● Accommodates 6mm, 12mm, 24mm cups
and Costar Snapwell cup
● Sterilized with EtO, alcohol or a bactericide
held STX2 electrodes) to less than 5 Ω. With Endohm’s fixed electrode
geometry, variation of readings on a given sample is reduced from 10-30
Ω with STX2 electrodes (depending on the experience of the user) to 1-2.
Compared with other resistance measurement methods, Endohm with
EVOM2 offers a much more convenient and economic solution to “leaky
tissue” measurement. Because of the uniform density of the AC square
wave current from EVOM2, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM2
offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Costar, Millipore, ICN Biomedicals, and Falcon
have been tested. Endohm chambers may be sterilized with EtO, alcohol or
a bactericide (also see: Cidex, Microsurgery section); not autoclavable.
Using WPI’s EVOM2 resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial tissue in culture cups. Transfer
cups from their culture wells to the Endohm chamber for measurement
rather than using hand-held electrodes. The chamber and the cap each
contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the
top electrode can be adjusted to fit cell culture cups of different
ENDOHM-6
End­ohm for 6 mm culture cup (24 wells per plate)
manufacture. Endohm’s symmetrically apposing circular disc
ENDOHM-12
Endohm for 12 mm culture cup (12 wells per plate)
electrodes, situated above and beneath the membrane, allow
ENDOHM-24SNAP Endohm for 24 mm & Costar Snapwell™ cup (6 wells per plate)
a more uniform current density to flow across the membrane
Requires EVOM2, EVOM or EVOMX to operate
than with STX2 electrodes. The background resistance of a
53330-01
Replacement Endohm Cable
blank insert is reduced from 150 Ω (when using WPI’s handWorld Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
15
EPITH ELIAL PHYS IOLOGY
Automated
TEER
Measuring
System
The REMS AutoSampler automates measurements of electrical
resistance of transepithelial, transendothelial or Caco-2 cell membranes
being grown to confluence on microporous filters of high throughput
screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue
resistance measurement system that offers reproducibility, accuracy,
flexibility and ease-of-operation for this kind of measurement. Automated
measurement of tissue resistance in cell culture microplates provides
the important advantages of speed, precision, decreased opportunity for
contamination and the instant availability of measured resistance data on
a computer. These measurements are useful in applications such as drug
bioavailability studies and studies on the mechanisms of drug transport.
The main components of the REMS AutoSampler include: the robotic
sampler that moves the electrode over each well of the microplate,
the electrode which is located on the robotic arm, a base plate for
the 24- and 96-well tray, a Windows-based data acquisition card, the
REMS interface unit and the REMS software to operate the system on a
Windows-based computer.
The REMS AutoSampler automates TEER measurements previously
made with WPI’s EVOM Epithelial Voltohmmeter. Automated tissue
resistance measurements up to 20 kΩ can be performed on 24- or 96-well
HTS microplates. Microplates presently supported include the Corning
Costar HTS Transwell-24, Falcon HTS Multiwell insert systems, and
Millipore Multiscreen™ CaCo 96-well plate.
REMS AUTOSAMPLER SPECIFICATIONS
MEMBRANE RESISTANCE RANGE
0 to 2000 Ω and 0 to 20 kΩ
AC SQUARE WAVE CURRENT
+/- 20 µA @ 12.5 Hz
ELECTRODE POSITIONING
Resolution in X, Y and Z: +/- 1 mm
ELECTRODE PERFORMANCE
Repeatability in X, Y and Z: +/- 0.25 mm
ELECTRODE ARM SPEED
X- and Y-axis: 250 mm/sec
Z-axis: 247.3 mm/sec
TYPICAL MEASUREMENT TIME
24-WELL
1 min, 10 sec
SCAN PATTERN
Choice of any well pattern sampling
LINE VOLTAGE
User specified: 100/120 V or 220/240 V
DIMENSIONS53.5 × 43.7 × 37.1 cm
(213⁄32 × 173⁄16 × 145⁄8 in.)
WEIGHT
24 kg (52 lb)
to reproducibly and precisely locate the electrode results in highly
reproducible TEER measurements. TEER measurements are stored in
the computer as the electrode moves from one well to the next. The
Windows-based software provides user-friendly features to acquire,
display and store the tissue resistance measurements.
The REMS AutoSampler is designed to facilitate integration with
The REMS electrode is very compact and robust in design. Each of two
other robotic systems. Special locating bars are installed on the REMS
rod-shaped probes, 1.5 mm in diameter, consists of a pair of electrodes:
base platform that allow other system robots to place an HTS tray into a
one electrode for injecting current and the other for measuring the
precise location on the REMS base.
voltage. The use of two pairs of electrodes eliminates the error caused
The REMS AutoSampler will automatically measure and record
by the electrode-liquid interface. To take a measurement, the robot
tissue resistance from a user-specified matrix of culture wells on the
inserts one probe into the center of the filter well and the other into the
microplate. According to the specified sequence, the robotic arm moves
opening slot of the 24- or 96-well plate. The use of AC current to measure
over the identified wells taking TEER measurements. By means of a x-y-z
resistance provides several advantages over DC current, including:
locating system, the electrode-containing arm is positioned precisely
● Absence of offset voltages on measurements;
and reproducibly over each well. The ability of the REMS AutoSampler
● There is a zero net current being passed through the membrane
and, therefore, it is not adversely affected by a
REMS
Automated Tissue Resistance Measuring System
current charge;
Includes robot sampler, base plate, data acquisition board; computer, display,
●
No electrochemical deposition of electrode
keyboard, mouse; software for Windows XP or Vista; and electrode for either
metal.
24-well plate (Corning Costar HTS Transwell-24 or Falcon HTS Multiwell) or 96-well
The REMS AutoSampler also features a rinse
plate (Millipore Multiscreen CaCo) — SPECIFY WHEN ORDERING.
and calibration check station. If occasional
ACCESSORIES
rinsing of the REMS electrode is required it may
REMS-24
Replacement REMS STX Electrode for 24-well HTS Plate be sent to a rinse station by pressing the rinse
REMS-96
Replacement REMS STX Electrode for MilliporeTM 96-well Plate
station button on the menu bar.
Contact WPI for detailed information.
World Precision Instruments
www.wpiinc.com
16
UK: Tel: +44 (0)1462 424700 • [email protected]
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Multi-Channel Voltage / Current Clamp
More channels and a wider range of voltage clamp commands than WPI’s classic
DVC‑1000. The superior design of the cartridge electrodes makes 100-volt current
excursion unnecessary, so this safe, low-voltage system is easier to adjust and use.
EVC4000 employs the voltage clamp technique to monitor membrane
permeability as a function of membrane voltage or applied chemicals.
When combined with WPI’s patented EKC and EKV cartridge electrodes,
EVC4000 can efficiently voltage or current clamp up to four sample
membranes simultaneously using safe moderate voltages on the current
wire leads. The superior design of the cartridge electrodes makes 100volt current excursion unnecessary, so this safe, low-voltage system is
easier to adjust and use. Extremely stable and accurate, each module,
with its companion preamplifier, can operate independently in one of
three different modes: Voltage Clamp (VC), Current Clamp (CC), or Open
Circuit Potential (PD) measurement. EVC4000 can be controlled from
the front panel of the instrument or from computer generated commands
applied at the rear panel of the instrument. A feature unique to EVC4000
is an electronic potentiostat in the preamplifier box that maintains the
serosal electrode invariant potential at zero relative to system ground.
The preamplifier apparatus actively maintains one surface of the test
membrane close to ground potential under all operating conditions.
EVC4000 SPECIFICATIONS
PREAMPLIFIER
Input Resistance
1012 Ohms
Input Leakage Current
100 pA, max. Maximum Input Voltage
±15 volts
VOLTAGE CLAMP Panel Display
±200 mV ±0.1 mV
Clamp Voltage / External Input
100 mV per Volt Range of Voltage Electrodes
±32 Volts Max. Clamp Voltage
±100 mV Fluid Resistance Compensation
0 to 1000 Ohms
CURRENT CLAMP
Panel Display
±999 µA ±1 µA
Maximum Clamp Current
±1 milliampere Current Clamp Output
1 µA / mV
DISPLAY RESOLUTION Voltage
0.1 mV Current
1 µA
DIMENSIONS
18.25 x 7.2 x 9.6 in.
(46 x 18 x 24 cm)
SHIPPING WEIGHT (EVC4000-4)
26 lb (12 kg)
References
W. K. MacNaughton (2000) “Role of constitutive cyclooxygenase-2 in
prostaglandin-dependent secretion in mouse colon in vitro.” Journal of
Pharmacology and experimental Therapeutics 293, 2, 539-544
EVC4000-4
EVC4000-3
EVC4000-2
EVC4000-1
4-Channel Volt­age Clamp & preamps (shown above)
3-Channel Voltage Clamp & preamps
2-Channel Voltage Clamp & preamps
1-Channel Voltage Clamp & preamp
Specify line voltage
OPTIONAL ACCESSORIES
SYS-EVC4000 Replacement Voltage Clamp & EVC3 Preamplifier
EVC3Replacement Preamplifier Module
EK1
Ussing Electrode Kit (2 voltage, 2 current)
EKV
Extra Ussing Voltage Electrode (each)
EKC
Extra Ussing Current Electrode (each)
2851
BNC Cable
3485
Post Mounting Kit for Preamp
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
17
EPITH ELIAL PHYS IOLOGY
EVC4000-4 (complete four-channel system)
EVC4000
EPITH ELIAL PHYS IOLOGY
Ussing
System
Glass
cir­cu­la­tion
reservoir
#5210
For electrophysiological investigation
of epithelial transport
● Direct connect low-resistance electrodes
● Simplified operation, easy to control temperature and
clean after use
Ussing
Chamber
● Luer type leak-free attachment of tubing and
electrodes
● Recessed electrode ports to avoid air bubble formation
● Secure membrane holding by sharp stainless steel pins
or O-ring
● Specialized chamber adapts cell culture insert (Costar Snapwell) for monolayer cell culture
● Chambers with rectangular openings for tubular
tissues from small animals
WPI’s Ussing System offers researchers a quick, effective means of
making low-resistance electrical connections to the Ussing chamber
without need of long agar bridges or Calomel half-cells. Ag/AgCl halfcells screw into short tubes which plug firmly into place in the chamber’s
luer ports. These direct-connect elec­trodes eliminate the inconvenience
and expense of Calomel half-cells in open liquids. The System includes
one Ussing Chamber (eight sizes available), Support Stand, Electrode Kit,
Glass Circulation Reservoir (two sizes available), and a Tub­ing start-up kit
(25 feet of 0.375-in. tubing, 10 feet of 0.156-in. tubing, plus four male
luer fittings, two compressor clamps, one Y-connector, and one clip). Six­
teen possible system configurations are list­ed at right. Com­po­nents are
also avail­able sep­a­rate­ly. (Preamplifier in pho­to not included.)
Ussing Chambers
18
Two small chambers with rectangular openings are designed for tubular tis­sue from small an­i­mals such as the mouse intestinal tract membrane (CHM6) and rat in­tes­ti­nal tract mem­brane (CHM7). The rect­an­gu­
lar open­ing more close­ly match­es the shape of the tissue than would a
circular open­ing, sig­nif­i­cant­ly increasing the mem­brane area available
for testing. The larger mem­brane area increases the transport rate of low
permeability chemicals; it also reduces the electrical re­sis­tance of the
system for easier current clamp­ing.
Optional Drains
Drains may be added to Ussing chambers to allow quick and complete
evac­u­a­tion of radioactive or tox­ic sub­stanc­es. To have drains added at
the time of or­der, add a “D” to the part number (such as “USS1LD”); cost
of the drain ($105 to $140) will be add­ed to the cost of the cham­ber or
system or­der­ed.
Cartridge Electrodes
The Electrode Kit contains four
voltage/current electrodes, plus four
luer-tipped cartridges. Elec­trodes are
thread­ed and screw securely into the
end of each car­tridge. The luer tip
then plugs securely into the luer open­
ings of the cham­ber. The ca­ble from
each elec­trode ter­mi­nates with a 2
mm pin which may be plugged into
EKV and EKC
voltage/current clamps such as WPI’s
Cartridge Elec­trodes
DVC1000 or EVC-4000.
The miniature electrode-gel cartridge is a small plastic tube with a male
luer tip identical to those at the tip of hy­po­der­mic syringes. The tube
may be filled with different gel ma­te­ri­als; agar is com­mon­ly used but
other gel ma­te­ri­als may also be sat­is­fac­to­ry.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
U.S. Patent No. 4,912,060
WPI’s classical Ussing Chambers are well established perfusion chambers
that are easy to operate, easy to control temperature, and easy to clean
after use. Hundreds of them are used daily by scientists in the field.
Ussing Cham­bers are machined from solid acrylic with eight entry ports
for fluid lines, elec­trodes, or agar bridges. For easy, leak-free attachment
of tubing and elec­trodes, all eight ports are luer type. The four ports
for volt­age and current electrodes are re­cessed to prevent formation of
air bubbles in the chamber. The fluid com­part­ments in each side of the
chamber are sep­a­rat­ed by the epithelial membrane being stud­ied. Sharp
stainless steel pins on one side of the chamber hold the mem­brane in
po­si­tion and mate with holes in the opposite chamber in­ter­face. (In the
CHM4, tissue is held by an O-ring instead of pins.)
The CHM5 chamber adapts the Costar Snapwell, a cell cul­ture in­sert
for mono­lay­er cell culture, into WPI’s “classical” ep­i­the­lial volt­age clamp
sys­tem. Until now, classical Ussing Cham­bers have not been widely used
for mono­lay­er cell culture in­serts be­cause most inserts have a very deep
pro­file, limiting good fluid per­fu­sion at the surface of the mem­brane —
and limiting voltage elec­trodes from measuring the po­ten­tial close to the
sur­face of the mem­brane. CHM5 solves these problems: Per­fu­sion fluid is
in­tro­duced into the chamber at an angle so that it will flow directly to the
surface of the mem­brane. The volt­age elec­trode is also in­sert­ed into the
cham­ber at an angle so as to reduce the distance between the surface of
the membrane and the electrode.
Complete Ussing System in­cludes stand, glass res­er­voir, electrodes,
Ussing chamber and tubing (EVC3 preamp and post mounting kit
not in­clud­ed—see page 18).
CHM8
CHM8 Chamber
Reservoir
openings and
pins
Fluid out
Electrode
9 mm
17 mm
12 mm
CHM4
Fluid in
CHM3
Current
Current
Electrode
Electrode
13.5 mm
6 mm
18.5 mm
CHM6
Luer ports allow easy fitting
of fluid lines and electrodes
14.5 mm
16.5 mm
12 mm
CHM7
30 mm
32 mm
9 mm
Alignment
Dots
CHM5
4 mm
7 mm
Guide
Pins
12 mm
EPITH ELIAL PHYS IOLOGY
Optional
Optional drain
drain
for
for hazardous
hazardous
material
material also
also
available
available
5.5 mm
7 mm
Suture quality pins
minimize tissue damage
CHM1
4 mm
5 mm
Clear acrylic chambers let you see
Voltage
your experiment in progress . . .
CHM2
0.5 mm
pins
Assembled chambers are 101.6 mm (4 in.) long.
CHM1
(Medium)
CHM2
(Small)
CHM3
(Large)
CHM4
(Ex­tra Small)
CHM5
(Snap)
CHM6
(Rect., Small)
CHM7
(Rect., Large)
CHM8
(Extra Small)
Reservoir Opening
12 mm
9 mm
13.5 mm
4 mm
12 mm
5 x 14.5 mm
7 x 30 mm
4 mm
Half-Chamber Volume
1.0 mL
0.75 mL
1.2 mL
0.5 mL
1.7 mL
0.8 mL
5.5 mL
0.5 mL
Pin Circle Diameter
17 mm
12 mm
18.5 mm
6 mm*
N/A
7 x 16.5 mm
9 x 32 mm
5.5 mm
*O-ring diam.
Circulation Reservoirs
Water Bath
Hand-blown boro­sil­i­cate glass, with
jacketed cham­bers for tem­per­a­ture con­
trol. Avail­able in two siz­es — #5210
holds 20 mL per side, and #5362 (at
left) holds 10 mL per side (use­ful when
ex­pen­sive chem­i­cals are in­volved). Res­er­
voir con­dens­er caps pre­vent air bub­bles
and tur­bu­lence in flu­id res­er­voirs.
The Julabo circulating bath (see next page) is
ideal for controlling temperatures of external
systems. With a powerful 15L/min
flow rate, the pump provides
optimum heat exchange.
The tap water cooling
feature is standard with a
range of 20-100ºC. The bath
opening is 15cm x 15cm x
15cm and can hold 3–4.5L
of liquid.
USSING SYSTEMS, LARGE RESERVOIR
USS1L
Medium Chamber, Stand, Reservoir, Electrodes, Tubing
USS2L
Small Chamber, Stand, Reservoir, Electrodes, Tub­ing
USS3L
Large Chamber, Stand, Reservoir, Electrodes, Tub­ing
USS4L
Extra Small Chamber, Stand, Reservoir, Electrodes, Tubing
USS5L
Snap Chamber, Stand, Reservoir, Electrodes, Tubing
USS6L
Small Rectangular Chamber, Stand, Reservoir, Electrodes, Tubing
USS7L
Large Rectangular Chamber, Stand, Reservoir, Electrodes, Tubing
USS8L
Extra Small Chamber, Stand, Reservoir, Electrodes, Tub­ing
USSING SYSTEMS, SMALL RESERVOIR
USS1S
Medium Chamber, Stand, Reservoir, Electrodes, Tubing
USS2S
Small Chamber, Stand, Reservoir, Electrodes, Tub­ing
USS3S
Large Chamber, Stand, Reservoir, Electrodes, Tub­ing
USS4S
Extra Small Chamber, Stand, Reservoir, Electrodes, Tubing
USS5S
Snap Chamber, Stand, Reservoir, Electrodes, Tubing
USS6S
Small Rectangular Chamber, Stand, Reservoir, Electrodes, Tubing
USS7S
Large Rectangular Chamber, Stand, Reservoir, Electrodes, Tubing
USS8S
Extra Small Chamber, Stand, Reservoir, Electrodes, Tub­ing
* Add EVC4000 at reduced price when buying Ussing System with
equivalent number of channels
EVC4000-1
EVC4000-2
EVC4000-3
EVC4000-4
1-Channel Voltage Clamp & Preamps
2-Channel Voltage Clamp & Preamps
3-Channel Voltage Clamp & Preamps
4-Channel Voltage Clamp & Preamps
* Drain option, $105 to $140, varies according to chamber.
System components also available separately:
xxxxD
Drain option (add “D” to part number of chamber or system)
CHM1
Medium Chamber
CHM2
Small Chamber
CHM3
Large Chamber
CHM4
Extra Small Chamber with O-Ring Seal
CHM5
Snap Chamber (fits Costar Snapwell cups)
CHM6
Small Rectangular Chamber
CHM7
Large Rectangular Chamber
CHM8
Extra Small Chamber with Mounting Pins
EK1
Ussing Electrode Kit (2 voltage, 2 current)
EKC
Extra Ussing Current Electrode (red) (each)
EKV
Extra Ussing Voltage Electrode (blue) (each)
5210
Large Glass Circulation Reservoir, (20 mL per side)
5233
Replacement Condenser for 5210
5362
Small Glass Circulation Reservoir, (10 mL per side)
5361
Replacement Condenser for 5362
3955
EKV Cartridges, 35 mm (pkg of 12)
3960
EKC Cartridges, 58 mm (pkg of 12)
3669
Tubing Kit (flexible hose and luer fittings)
3579-20
Replacement luer fittings for tubing connections (pkg of 20)
5153
Support Stand
3485
Post Mounting Kit for Preamp
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
19
Circulating Bath
JULABO BATH Specifications
Temperature Selection. . . . . . . . . . . . . . . . . . . . . digital
Temperature Indication. . . . . . . . . . . . . . . . . . . . . . . LED
Resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.1ºC
Temperature Control. . . . . . . . . . . . . . . . . . . . . . . . . PID1
Heater Wattage . . . . . . . . . . . . . . . . . . . . 230V–2.0kW
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115V–1.0kW
Circulating Pump
Discharge, max a 0 bar . . . . . . . . . . . . . . . . . . 15L/min
Pressure, max. at 0 I. . . . . . . . . . . . . . . . . . . . . 0.35 bar
Ambient Temperature. . . . . . . . . . . . . . . . . . . . . . 5-40ºC
Mains Power Connection (230V/50Hz). . . 190-253V/50Hz
Current Input (230V). . . . . . . . . . . . . . . . . . . . . . . . . . 9A
Mains Power Connection (115V/60Hz). . . 103-127V/60Hz
Current Input (115V). . . . . . . . . . . . . . . . . . . . . . . . . . 9A
Bath Tank. . . . . . . . . . . . . . . . . . . . . . . . . . Stainless steel
Working Temperature Range. . . . . . . . . . . . . 20-100ºC
Temperature Stability. . . . . . . . . . . . . . . . . . . . . . ±0.03
Bath Opening (WxL). . . . . . . . . . . . . . . . . . . 15 x 15cm
Bath Depth. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15cm
Filling Volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4.5L
Dimensions . . . . . . . . . . . . . . . . . . . . . 17 x 33 x 36cm
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.0kg
Recommended Bath Fluid. . . . . soft/decalcified water
EPITH ELIAL PHYS IOLOGY
The Julabo circulating bath is ideal for controlling temperatures of external systems. With a
powerful 15L/min flow rate, the pump provides
optimum heat exchange. The tap water cooling
feature is standard with a range of 20-100ºC.
The bath opening is 15cm x 15cm x 15cm and
can hold 3–4.5L of liquid.
l LED temperature display (0.1ºC resolution)
l Stainless steel bath tank
l Adjustable high temperature cut out and dry
running protection.
l PID temperature control
l Large capacity for temperature applications with larger external systems and open
systems
l Internal bath for simultaneous applications
with smaller objects
l Built in cooling coil for tap water connection
when you require a temperature less than the
ambient temperature
503843
503844
504122
504142
504141
Julabo Circulating Bath, 115V
Julabo Circulating Bath, 230V
Julabo Circulating Bath, 100V (Japan)
Julabo Circulating Bath, 13L volume, 110V
Julabo Circulating Bath, 13L volume, 220V
Rodent Brain Matrices
WPI offers one of the largest selections of
brain matrices available. Made of acrylic, or
stainless steel, these matrices are sturdy and
can be heated, chilled, autoclaved (stainless
steel only), scrubbed — and stand up to
rigorous daily use. Coronal matrices have
the additional feature of a mid-line sagittal
cut to facilitate splitting of the left and right
hemispheres. Sections can be as fine as
1‑mm. The olfactory/spinal/notch is cut into
each matrix.
D
A B
c
E
20
Order #
Subject
Material
Section
A
B
C
D
E
Cavity
Depth
Weight
RBMA-200C
Adult Mouse, 40-75g
Acrylic
Coronal
3.18
11.1
8.73
19.1
12.2
7.4
0.5 lb
RBMA-200S
Adult Mouse, 40-75g
Acrylic
Sagittal
3.18
11.1
8.73
19.1
12.2
7.4
0.5 lb
RBMA-300C
Rat, 175-300g
Acrylic
Coronal
4.7
15.9
12.7
36.6
23.8
7.61
0.5 lb
RBMA-300S
Rat, 175-300g
Acrylic
Sagittal
4.76
15.9
12.7
36.6
23.8
10.91
0.5 lb
RBMA-600C
Rat, 300g-600g
Acrylic
Coronal
4.76
19.8
14.7
36.6
24.7
10.91
0.5 lb
RBMA-600S
Rat, 300g-600g
Acrylic
Sagittal
4.76
19.8
14.7
36.6
24.7
10.91
0.5 lb
RBMS-200C
Adult Mouse
Stainless Steel
Coronal
3.18
11.1
8.73
19.1
12.2
7.4
1.0 lb
RBMS-200S
Adult Mouse
Stainless Steel
Sagittal
3.18
11.1
8.73
19.1
12.2
7.4
1.0 lb
RBMS-300C
Rat, 175-300g
Stainless Steel
Coronal
4.76
15.9
12.7
36.6
23.8
7.61
1.0 lb.
RBMS-300S
Rat, 175-300g
Stainless Steel
Sagittal
4.76
15.9
12.7
36.6
23.8
7.61
1.0 lb
RBMS-600C
Rat, 300g-600g
Stainless Steel
Coronal
4.76
19.8
14.7
36.6
24.7
10.91
1.0 lb
RBMS-600S
Rat, 300g-600g
Stainless Steel
Sagittal
4.76
19.8
14.7
36.6
24.7
10.91
1.0 lb
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
The instruments shown in this section are a sampling of the most
popular tools from WPI. For a complete look at the hundreds of
other items available, call today for the complete catalog.
Forceps by Dumont
Dumont #3
Stainless steel
12 cm (4.75 in.)
0.20 x 0..12 mm tips
503235 . . . . . . . . . . . . . . . . . . . .
1:1
Dumont #5
Material: Dumostar, non-magnetic, non-corrosive stainless steel
Length:
11 cm long (4.75 in.)
Tips:
0.025 x 0.015 mm
Tip Profile:
500085 . . . . . . . . . . . . . . . . . . . .
1:1
Dumont #5
Material:
Length:
Tips:
Finest tips
available
Stainless steel
11 cm (4.3 in.)
0.025 x 0.005 mm
501985 . . . . . . . . . . . . . . . . . . . .
1:1
Dumont #5
Material:
Length:
Tips:
Dumostar, non-magnetic, non-corrosive
11 cm (4.3 in.)
0.1 x 0.06 mm Tips
500233 . . . . . . . . . . . . . . . . . . . 1:1
Tip Profile:
Dumont #5
Material: Dumoxel, non-magnetic stainless steel
Length: 11 cm (4.3 in.)
Tips:
0.1 x 0.06 mm tips
14098 . . . . . . . . . . . . . . . . . . . . Tip Profile:
ST
MO
R!
U LA
P
O
P
1:1
Dumont #5
Material:
Length:
Tips:
Stainless steel
11 cm (4.3 in.)
0.10 x 0.06 mm
500342 . . . . . . . . . . . . . . . . . . . .
1:1
Tip Profile:
Dumont #5
Material: Stainless steel, Biology
Length: 11 cm (4.3 in.)
Tips:
0.05 x 0.01 mm tips
500341 . . . . . . . . . . . . . . . . . . . 1:1
Tip Profile:
Dumont #5
Material:
Length:
Tips:
Stainless steel, Medical Biology
11 cm (4.3 in.)
0.05 x 0.01 mm
14095 . . . . . . . . . . . . . . . . . . . . .
1:1
Tip Profile:
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
21
MICRODISSECTION, MICROSURGERY
Material:
Length:
Tips:
For material information and instrument
purchase guidance visit www.wpiinc.com
Dumont #5
Material:
Length:
Tips:
Stainless steel, Medical Biology, bent at 45°
11 cm (4.3 in.)
Tip Profile:
0.05 x 0.01 mm
1:1
14101 . . . . . . . . . . . . . . . . . . . . Dumont #5
Material:Titanium
Length:
11 cm (4.3 in.)
Tips:
0.1 x 0.06 mm
Tip Profile:
14096 . . . . . . . . . . . . . . . . . . . . 1:1
Dumont #55
MICRODISSECTION, MICROSURGERY
Material:
Length:
Tips:
Stainless Steel, Biology
11 cm (4.3 in.)
0.05 x 0.01 mm
14099 . . . . . . . . . . . . . . . . . . . . Tip Profile:
1:1
Dumont #5B
Material:
Length:
Tips:
Stainless steel, Biology, bent at 45°
11 cm (44.3 in.)
0.05 x 0.01 mm
Tip Profile:
500234 . . . . . . . . . . . . . . . . . . . 1:1
Dumont #7
Material:
Length:
Tips:
Stainless steel
12 cm (4.75 in.)
0.17 x 0.1 mm
14097 . . . . . . . . . . . . . . . . . . . . .
Tip Profile:
1:1
Economy Fine Tweezers
Made of quality stainless steel, these tweezers are useful for
general dissections where ultrafine tips are not necessary.
Economically priced per package of 6, they are affordable
enough for everyone to have their own instruments.
Hundreds of additional
surgical instruments
and related items
are available. See the
complete catalog.
Economy Tweezer Style 3
Material:
Length:
Tips:
Stainless Steel
11 cm (4.3 in.)
0.20 x 0.40 mm
1:1
501976-6. . . . . . . . . . . . . . . . . .
501976 . . . . . . . . . . . . . . . . . . . .
Economy Tweezer Style 5
Material:
Length:
Tips:
Stainless Steel
11 cm (4.3 in.)
0.40 x 0.45 mm
501979-6. . . . . . . . . . . . . . . . . .
1:1
501979 . . . . . . . . . . . . . . . . . . . .
Economy Tweezer Style 7
Material:
Length:
Tips:
Stainless Steel
11 cm (4.3 in.)
0.40 x 0.50 mm
501981-6. . . . . . . . . . . . . . . . . .
1:1
501981 . . . . . . . . . . . . . . . . . . . .
22
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Forceps
Iris Forceps, serrated
Stainless steel
10 cm (4 in.) long, straight 0.8 mm tips
Tip Profile:
StandardGerman
15914 . . . . . . . . . . . . . . . . 1:1
15914-G . . . . . . . . . . . . . . . Iris Forceps, serrated
Stainless steel
10 cm (4 in.) long, curved 0.8 mm tips
Tip Profile:
StandardGerman
15915-G . . . . . . . . . . . . . . .
Adson Forceps, serrated
Stainless steel
12 cm (4.75 in.) long
1:1
1:1
Tip Profile:
Standard 14226 . . . . . . . . . German 14226-G . . . . . . . Tip Profile:
1:1
Hartman Mosquito Hemostatic Forceps
Stainless steel
Tip Profile:
Standard
German
15920
12.5 cm (5 in.) long, straight . . . . . . . . . 15920-G. . . . . . . . . . . . .
15921
12.5 cm (5 in.) long, curved . . . . . . . . . 15921-G. . . . . . . . . . . . .
501705
9 cm (3.5 in.) long, straight . . . . . . . . . . 501705-G. . . . . . . . . . . .
501291
9 cm (3.5 in.) long, curved . . . . . . . . . . 501291-G. . . . . . . . . . . .
Kelly Hemostatic Forceps
Stainless steel
Standard
German
501241 Straight, 14 cm (5.5 in.) long . . . . . . . . . 501241-G . . . . . . . . . . 501288 Curved, 14 cm (5.5 in.) long . . . . . . . . 501288-G . . . . . . . . . . 501714 Straight, 15.5 cm (6 in.) long . . . . . . . . . 501714-G . . . . . . . . . . 501715 Curved, 15.5 cm (6 in.) long . . . . . . . . . 501715-G . . . . . . . . . . Tip Profile:
1:1
Tip Profile:
Hundreds of additional
surgical instruments
and related items
are available. See the
complete catalog.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
23
MICRODISSECTION, MICROSURGERY
15915 . . . . . . . . . . . . . . . . Spring Scissors
These squeeze-handle micro scissors can be used ambidextrously.
Vannas Scissors, Super Fine, stainless steel
Length: 8 cm
Blades: straight 3 mm
Tips: 0.015 x 0.015 mm
Tip Profile:
501778 . . . . . . . . Finest Tips in the World!
Length: 8 cm
Blades: curved 3 mm
Tips: 0.015 x 0.015 mm
501839 . . . . . . . . 1:1
MICRODISSECTION, MICROSURGERY
Vannas Scissors, stainless steel
Length: 8.5 cm (3.3 in.)
Blades: straight 7mm Tips:
0.025 x 0.015 mm
Length: 8.5 cm (3.3 in.)
Blades: curved 7mm Tips:
0.025 x 0.015 mm
500086 . . . . . . . . . . . . . . . Tip Profile:
1:1
501232 . . . . . . . . . . . . . . . Vannas Scissors
Length:
Blades:
Tips:
Tip Profile:
8 cm (3 in.)
straight 5 mm
0.1 mm tips
1:1
StandardGerman
14003 . . . . . . . . . . . 14003-G . . . . . . . . . . . Vannas Scissors
Length:
Blades:
Tips:
Tip Profile:
8 cm (3 in.)
curved, 5 mm
0.1 mm tips
StandardGerman
14122 . . . . . . . . . . . . 14122-G . . . . . . . . . . . . 1:1
Vannas Scissors
Length:
Blades:
Tips:
Tip Profile:
8 cm (3 in.)
45° angled to side, 5 mm
0.1 mm tips
1:1
StandardGerman
500260 . . . . . . . . . . 500260-G . . . . . . . . . . McPherson-Vannas Scissors
Length:
8 cm (3 in.)
Blades:
straight 5 mm
Tips:0.1mm
Length:
8 cm (3 in.)
Blades:
curved 5 mm
Tips:0.1mm
Tip Profile:
StandardGerman
14124 . . . . . . . . . . . 14124-G . . . . . . . . 501234 . . . . . . . . . . 501234-G . . . . . . . 1:1
Spring Scissors
Length: 12 cm (4.75 in.)
Blades: straight 12 mm extra fine and long
Standard
14125 . . . . . . . . . . . . . German
14125-G . . . . . . . . . . . Length: 12 cm (4.75 in.)
Blades: curved 12 mm extra fine and long
14126 . . . . . . . . . . . . . 24
1:1
Tip Profile:
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Mini-Dissecting Scissors, stainless steel
503666
8.5 cm (3.3 in.) curved, sharp tips . . . . . . 503667
8.5 cm (3.3 in.) straight, sharp tips . . . . . 503668
8.5 cm (3.3 in.) curved, blunt tips . . . . . . 503669
8.5 cm (3.3 in.) straight, blunt tips . . . . . Tip Profile:
2.2 cm
1:1
Dissecting Scissors
Standard
14393
14394
15922
15923
German
14393-G . . . . . . . . . . 14394-G . . . . . . . . . . 15922-G . . . . . . . . . . 15923-G . . . . . . . . . . 10 cm (4 in.) straight . . . . . . . . . 10 cm (4 in.) curved . . . . . . . . . . 12.5 cm (5 in.) straight . . . . . . . . 12.5 cm (5 in.) curved . . . . . . . . Tip Profile:
Tip Profile:
Length:
11.5 cm (4.5 in.)
Blades:
straight, Tungsten Carbide
Standard
German
500216 . . . . . . . . . . Length:
Blades:
2.3 cm
1:1
500216-G . . . . . . . . . . . 11.5 cm (4.5 in.)
curved, Tungsten Carbide
500217 . . . . . . . . . . Tip Profile:
500217-G. . . . . . . . . . . .
Iris Scissors, stainless steel
Length: 11 cm (4.3 in.)
Standard
Blades: straight
German
501758 . . . . . . . . . . Length: 11 cm (4.3 in.)
Tip Profile:
501758-G. . . . . . . . .
2.2 cm
Blades: curved
501759 . . . . . . . . . . 1:1
501759-G. . . . . . . . .
Mini-Iris Scissors
503670
503671
Tip Profile:
8 cm (3.1 in.) straight, sharp tips . . . . . . . . 8 cm (3.1 in.) curved, sharp tips . . . . . . . . . Iris Scissors, SuperCut, stainless steel
one edge micro serrated, one edge honed to the sharpness of a knife edge
10 cm (4 in.)
Standard
German
Straight
14218 . . . . . . . . 14218-G . . . . . . Curved
14219 . . . . . . . . 14219-G . . . . . . 11.5 cm (4.5 in.)
Straight
503259 . . . . . . .
Curved
503260 . . . . . . . 1:1
2.3 cm
Shown: 10 cm, straight
Tip Profile:
Hundreds of additional surgical
instruments and related items
are available. See the complete
catalog.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
25
MICRODISSECTION, MICROSURGERY
Iris Scissors, stainless steel
Operating Scissors, stainless steel
11.5 cm (4.5 in.) Straight
StandardGerman
501753Sharp/Blunt . . . . . . . . . . . . . . . . 501753-G . . . . . . . . . 501754Sharp/Sharp . . . . . . . . . . . . . . . . 501754-G . . . . . . . . . 501743Blunt/Blunt . . . . . . . . . . . . . . . . . 501743-G . . . . . . . . . 4 cm
MICRODISSECTION, MICROSURGERY
11.5 cm (4.5 in.) Curved
501755Sharp/Sharp . . . . . . . . . . . . . . . . 501755-G . . . . . . . . . 501756Sharp/Blunt . . . . . . . . . . . . . . . . 501756-G . . . . . . . . . 501757Blunt/Blunt . . . . . . . . . . . . . . . . . 501757-G . . . . . . . . . 14 cm (5.5 in.) Curved
501220Sharp/Sharp . . . . . . . . . . . . . . . . 501220-G . . . . . . . . . 501221Sharp/Blunt . . . . . . . . . . . . . . . . 501221-G . . . . . . . . . 501222Blunt/Blunt . . . . . . . . . . . . . . . . . 501222-G . . . . . . . . . 14 cm (5.5 in.) Straight
14192Sharp/Blunt . . . . . . . . . . . . . . . . 14192-G . . . . . . . . . . . 501218Sharp/Sharp . . . . . . . . . . . . . . . . 501218-G . . . . . . . . . 501219Blunt/Blunt . . . . . . . . . . . . . . . . . 501219-G . . . . . . . . . 14192
Sharp/Blunt
Tip Profiles:
Sharp/Sharp
Blunt/Blunt
1:1
Needle Holders
Tip Profile:
Needle Holder
12.5 cm (5 in.) long
straight serrated jaws
extra delicate
Standard 14109 . . . . . . . .
German 14109-G . . . . . .
1:1
Hundreds of additional
surgical instruments
and related items
are available. See the
complete catalog.
26
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Clips and Clamps
Reflex Clip Applier
11 cm (4.3 in.) long
for 9 mm clips only
500345 . . . . . . . . . . Reflex Clip Applier
11 cm (4.3 in.) long
for 7 mm clips only
500343 . . . . . . . . . . Reflex Clip 7 mm
for use with #500343
100/box, Stainless Steel, non-sterile
500344 . . . . . . . . . . MICRODISSECTION, MICROSURGERY
Reflex Clip 9 mm
for use with #500345
100/box, Stainless Steel, non-sterile
500346 . . . . . . . . . . Micro Bulldog Clamps
Retractors
14119-G
Wire Retractors
5 cm (2 in.) long
10 x 5 mm blades
5 mm depth
maximum spread 13 mm
14119
Curved serrated jaws
Standard
Standard 14119 . . . . . . . . . .
32mm long, 12mm jaw
German German 14130 . . . . . . . . . .
14130-G . . . . . . . .
14119-G . . . . . . . .
38mm long, 9mm jaw
Scalpels, Knives, Blades and Handles
Scalpel Handle #3
13 cm (5 in.) long, stainless steel
Standard 500236 . . . . . . . . . . .
German 500236-G . . . . . . . .
For Blades #10 ~ #15
Sterile stainless steel blades
All stainless steel scalpel blades are made by Feather®, using a precise
beveling technique to create the edge’s micron sharpness. They are the
finest blades available.
500239
500240
500353
Scalpel Blades #10, stainless steel, sterile (100 / box) Scalpel Blades #11, stainless steel, sterile (100 / box) Disposable Scalpel, No. 21, sterile, (10/box)
Hundreds of additional surgical
instruments and related items
are available. See the complete
catalog.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
27
Ear Punches and Ear Tags
Ear Punch
5 cm (2 in.) long, 2 mm diameter
1:1
500075 . . . . . . . . . . MICRODISSECTION, MICROSURGERY
1:1
Ear Punch
10 cm (4 in.) long
2 mm diameter
1 mm diameter
Hundreds of additional surgical
instruments and related items
are available. See the complete
catalog.
500077 . . . . . . . . 500076 . . . . . . . . Mouse Kit
Dumont #5 (#14098)
Vannas Scissors (#14003)
Iris Forceps, curved, serrated (#15915)
Dissecting Scissors, straight 10cm (#14393)
Wire Retractor (#14130)
Needle Holder (#14109)
Blunt Probe, 1.0 mm diameter (#501313)
Order Number: MOUSEKIT
Now includes
storage case!
Student
Dissecting Kit
Includes:
Dissecting Scissors, 4.5”
Dissecting Scissors, 4”
Dressing Forceps, 5.5”
Micro Dressing Forceps, 4”
#4 Knife Handle
Straight Teasing Needle
Angled Teasing Needle
#22 Surgical Blade
Canvas Roll-up
28
501336 . . . . . . . . . . . . . . . . . . . . . . . 501838 Canvas Roll-up only . . . . . . .
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Rodent
Guillotine
Guillotine for Rodents and other small animals (opening 1.5 x 1.5 in.)
Guillotine for large rodents and other medium animals
(opening 2.5 x 2.5 in.)
Guillotine for larger animals (opening 4” x 4”)
The small animal guillotine has been completely
redesigned for ease of use and extra added
safety features. The blades are drawn together by
magnetic force to ensure a clean and precise cut
through very strong bones and skin.
There is a large base for stability, long handle for
extra leverage, spring action so the blades can
not fall down unexpectedly, hardened stainless
blades for endurance, simplified construction for
easy maintenance. The fluoropolymer coated surface on the base makes
cleaning easy.
The guillotine is considered one of the most humane methods to sacrifice
a subject.
OmniDrill35
This line-powered micro drill will make easy work of grinding, finishing,
cutting, and drilling bone, teeth, and other material. The high-torque 35,000
rpm (maximum) motor is quiet and has minimal vibration which reduces
wear on the motor and provides greater comfort for the user. It also features
a forward and reverse switch, “E Type” handpiece, and handpiece holder. The
handpiece has a removable nose cone that can be cleaned and sterilized. It
accepts 3/32” and 2.33 mm bur shanks. Unlike battery-powered drills, this
unit will maintain consistent power for the duration of use. The wide range of
speeds allows the user to control the amount of heat generation.
The following accessories are included with the Micro Drill System:
Qty
4
1
1
1
1
1
1
1
1
1
1
1
4
1
1
Description
Abrading Tip, Rubber
Abrading Tip, Stone
Accessory Stand
Ball Mill, Carbide, #1, .031” Diameter
Ball Mill, Carbide, #2, .039” Diameter
Ball Mill, Carbide, #3, .047” Diameter
Ball Mill, Carbide, #4, .055” Diameter
Ball Mill, Carbide, #5, .063” Diameter
Ball Mill, Carbide, #6, .071” Diameter
Ball Mill, Carbide, #7, .083” Diameter
Ball Mill, Carbide, #1/4, .019” Diameter
Ball Mill, Carbide, #1/2, .027” Diameter
Cutoff Disk
Mandrel, Screw
Mandrel, Threaded
Size
Ball Diameter (mm)
1/4
0.5
1/2
0.7
1
0.8
2
1.0
3
1.2
4
1.4
5
1.6
6
1.8
7
2.1
OmniDrill35 SPECIFICATIONS
INPUT
110V, 50/60 Hz
OUTPUT
0-32 Vdc
FUSE1 amp
OPERATING SPEED RANGE
0-35,000RPM
DIMENSIONS
178 x 114 x 89mm (7 x 4.5 x 3.5in.)
WEIGHT
1.7kg (3.75lbs)
8
2.3
503598
OmniDrill35 Micro Drill System, 110 V
503599
OmniDrill35 Micro Drill System, 220 V
Replacement Accessories
501850
Abrading Tip, Rubber, pk of 20
501851
Abrading Tip, Stone, pk of 5
501852
Accessory Stand
501853
Ball Mill, Carbide, #1, .031” Diameter, pk of 5
501854
Ball Mill, Carbide, #2, .039” Diameter, pk of 5
501855
Ball Mill, Carbide, #3, .047” Diameter, pk of 5
501856
Ball Mill, Carbide, #4, .055” Diameter, pk of 5
501857
Ball Mill, Carbide, #5, .063” Diameter, pk of 5
501858
Ball Mill, Carbide, #6, .071” Diameter, pk of 5
501842
Ball Mill, Carbide, #7, .083” Diameter, pk of 5
501860
Ball Mill, Carbide, #1/4, .019” Diameter, pk of 5
501861
Ball Mill, Carbide, #1/2, .027” Diameter, pk of 5
501862
Cutoff Disk, pk of 20
501863
Mandrel, Screw, pk of 5
501864
Mandrel, Threaded, pk of 5
502237
Stereotaxic Holder for OmniDrill35 Microdrill
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
29
MICRODISSECTION, MICROSURGERY
DCAP
DCAP-M
DCAP-L
● Large, stable base
● Hardened blades for
long service
● Ambidextrous
configuration
MICRODISSECTION, MICROSURGERY
Fantastic Magnifying Glasses!
S
urgical loupes help to alleviate eye strain
by enlarging the image when you are
working on tiny subjects or conducting
precision operations. They are portable and
easier to use than a surgical microscope.
However, they are not created equal, and
choosing the pair that’s right for you is important
to your satisfaction.
Choosing Loupes
Choosing the correct surgical loupes for your
application involves several factors, including
resolution, working distance, field of view, depth
of field, magnification, weight and interpupillary
distance. These terms are defined below.
Ideally, you want the lowest magnification that
is suitable for your application. As a general
rule, the lower the magnification, the greater
the depth of field and field of vision. Likewise,
the longer the working distance, the greater the
field of view. The larger your field of view, the
less you need to turn your head. This reduces
eye strain and fatigue. It is also important to
consider the weight and fit of your loupes.
Lightweight loupes are more comfortable for
longer periods of use, and they are less likely to
slide down your nose as you work. WPI loupes
have adjustable interpupillary distance for a
correct fit every time.
Three styles of loupes are available
today. The first is a single lens
loupe for simple, low-magnification
applications. A photographer or
jeweler might use this style. The
second style is the Galilean loupe
designed by the 17th-century
astronomer Galileo Galilei. Galilean loupes
use multiple lenses and offer magnification
between 2.0x and 3.0x. These are easy to
use, lightweight and affordable. For greater
magnification up to about 8.0x, prismatic loupes
(Keplerian) are available. Designed by Johannes
Kepler, prismatic loupes use a series of lenses
and prisms to magnify the subject. They offer
greater magnification, sharp resolution and a
greater depth of field.
WD
WD
(when sitting) (when standing)
Your Height
<5'7" (170cm)
34cm (14")
42cm (16")
5'7"-6'4" (170-193cm)
42cm (16")
50cm (20")
>6'4"(193cm)
50cm (20")
55cm (22")
Things to Consider
Resolution
Resolution determines the amount of fine
details that can be distinguished. The type of
glass used in the lenses and coatings applied
to it can affect the resolution of your loupe.
To test a set of loupes, look through them at a
piece of graph paper. Notice color distortions or
curvature of the lines. A high resolution loupe
will have crisp, straight lines. The lines, seen
through lower quality lenses, will be slightly
blurred and curved.
500370 — 2.5x Binocular Loupe
30
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Working Distance
The working distance is distance at which a
loupe will focus. The working distance must be
equal to the distance from the loupe lens to the
top of your subject. Each loupe has a defined
working distance, but the working distance you
require will depend upon your height, posture
and table height. It is best to determine your
desired working distance, and then choose a set
of loupes that will meet your criteria.
You can measure your actual working distance
or use the table at left to get a rough idea.To
measure your working distance, sit or stand in
a comfortable position with your back straight.
Do not lean forward too much. Measure the
distance from your eyes to the top of your
subject. Usually, your working distance will be
close to what is shown in the table.
504053 — 4.5x Binocular Loupe
The area that is in focus when viewed through
the loupes is the field of view. The longer the
working distance of a loupe, the greater its
field of view will be. Likewise, the lower the
magnification factor, the larger the field of view.
This tradeoff must be considered carefully when
choosing loupes. When using a Galilean loupe
the center of the image is clear, but the outer
rim of the image is blurred. The prism loupe
image is sharp to the very edge of the field of
view.
Depth of Field
Like the field of view, the depth of field is
directly related to the working distance and
magnification factor. The depth of field is the
amount of depth that is in focus when viewing
the subject through the loupe. Greater depth of
504040 — 3x loupes flip up to allow use
of prescription lenses (installed by user’s
ophthalmologist).
504038 — 2.5x Binocular Loupe
BINOCULAR LOUPES
PART #
PRISM
POWER
WORKING
DISTANCE
503645
503646
501331
503622
504052
504053
504054
500370
503640
504037
504038
504039
503641
503642
504040
504059
504060
✔
✔
✔
✔
✔
✔
✔
3.5x
3.5x
4.0x
4.0x
4.0x
4.5x
4.5x
2.5x
2.5x
2.5x
3.5x
2.5x
3.0x
3.0x
3.0x
3.5x
3.5x
34 cm
50 cm
34 cm
50 cm
50 cm
34 cm
50 cm
35 cm
50 cm
34 cm
50 cm
34 cm
34 cm
50 cm
50 cm
34 cm
50 cm
TTL
field is preferred, because you
can see deeper into the subject
without repositioning. For greater
depth of field, choose a loupe
with a longer working distance
or a lower magnification factor.
Magnification
The size of the image viewed
through the loupe is determined
by the amount of magnification,
which is a personal preference.
Higher magnification provides a
larger image, but it also means a
reduced field of view and depth
of field. Generally speaking,
for simple surgical cases or
for beginners in training,
2.5x or 3.0x magnification is
sufficient. When a more delicate
or complicated procedure is
required, choose a magnification
factor of 3.5x to 4.5x. If you
are working on a tiny area or
are conducting a microsurgery,
chose a magnification factor of 5.0x to 6.0x.
Higher magnification loupes can be used in
place of a surgical microscope, if desired.
Interpupillary Distance
The distance between the pupils of your two
eyes is your interpupillary distance. For the sake
of comfort when focusing, your loupes must
fit your eyes. You optometrist can give you an
exact measurement. WPI loupes are adjustable
so that you can set your loupes to an interpupillary distance that suits you.
504037 — 2.5x loupes clip
onto the user’s glasses.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
31
MICRODISSECTION, MICROSURGERY
Field of View
BioClave Mini
Research Autoclave
●● Fully automatic–just press START
●● Rapid, complete sterilization
●● Extremely compact
MICRODISSECTION, MICROSURGERY
●● Built in water tank does not
require connection to an external
water supply
●● Benchtop model
The BioClave Mini comes pre-programmed for basic
sterilization. Choose either the 120ºC or 134ºC
temperature mode, and the cycle time is fixed. This is
the perfect autoclave for small spaces.
The stainless steel sterilization chamber
accommodates a variety of liquids, media,
BIOCLAVE MINI Specifications
Chamber Volume. . . . . . . . . . . . . .
Max. Pressure. . . . . . . . . . . . . . . . .
Chamber Dimensions. . . . . . . . . .
Tray Dimensions . . . . . . . . . . . . . .
Exterior Dimensions. . . . . . . . . .
Weight. . . . . . . . . . . . . . . . . . . . . . . .
Electrical. . . . . . . . . . . . . . . . . . . . .
8L
29 PSI/2 Bar
6.7 x 12.5"/17 x 31cm
4.75 x 9.75"/12 x 24.8cm
20 x 13.5 x 12.9"/51 x 24 x 33cm
74 Lb. (33kg)
115 or 230V, 50–60Hz, 950W
instruments, glassware and plasticware. A mechanical and electrical
safety interlock prevents the door from being opened until the pressure is
released (0 PSI).
Simply press the Start button, and the entire sequence runs–fill, sterilize,
exhaust and dry.
504187
504188
Sterilizing Trays
501729
Fine surgical instruments are too valuable not to protect. WPI’s sterilizing
trays help reduce damage from everyday use. With top quality structural
Integrity, these trays are ideal for the handling and storage of all standard
microdissection and surgical instruments.
BioClave Mini (115 V)
BioClave Mini (230 V)
Dry Sterilizer
Sterilize your
microdissecting and
tissue culture in­stru­
ments, thoroughly and
conveniently, in sec­onds.
No chem­i­cals. No flames.
No risk of burns. No
disinfectant fluids. Glass
beads heated to 260°C
kills all viruses, aer­o­bic
and anaer­o­bic bac­te­ria,
yeasts and spores. (1.5
mm lead-free glass beads
in­cluded.)
● GE’s ULTEM® resin ensures product strength, structural integrity and
extended life cycle.
● See-through lids make it easy to locate the right instrument.
● Suitable for all methods of sterilization (autoclave, steam, dry heat and
chemical).
Germinator Specifications
O.D. . . . . . . . . . . . . . . . . . 17.1 x 13.3 x 12.9 cm (6 /4 x 5 /4 x 5 /16 in.)
I.D. . . . . . . . . . . . . . . . . . . 5.1 x 10.2 cm (2 x 4 in.)
WEIGHT . . . . . . . . . . . . . . 2.3 kg (5 lb.)
3
● Unique “grid” design in base makes it easy to install the silicone finger mat
required to protect the delicate instruments.
500252
500253
500254
501728
501729
500255
32
Sterilizer tray, 15 x 6 x 2 cm, base, silicone mat, lid
Sterilizer tray, 19 x 10 x 2 cm, base, silicone mat, lid
Sterilizer tray, 25.5 x 15 x 2 cm, base, silicone mat, lid
Sterilizer Tray, 25.5 x 15 x 4 cm, base, insert tray, lid, 2 mats
Sterilizer Tray for Knife 15 x 6 x 2 cm, base, lid, 2 bars
Silicone mat, 38 x 25.5 cm
14404
500121
14405
1
1
Dry Sterilizer (110 V)
Dry Sterilizer (220 V)
Extra Glass Beads
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
The PI-MC2010 is a micro-cauterizing unit
that embraces thermodynamic technology to
perform micro-surgical tasks with efficiency and
cleanliness. Cells just beyond the cutting line are
left healthy and functional.
How It Works
A current passes through the platinum cutting
wire and super-heats it at a preprogrammed
rate for the span of a few microseconds, then
abruptly stops. A thin, gaseous-tube of solution
surrounding the wire is created because the rate
of heating is much faster than the velocity of the
solution molecules due to their average thermal
energy at room temperature. This creates an
intense but finite shockwave as the superheated
tube of vapor expands from the wire inducing
turbulence. The quick heating and cooling of
Tips available with 13 µm or 18 µm wire and with
1 mm or 1.5 mm loop.
the platinum wire causes a flushing effect as
cool water rushes in after the brief shockwave
and turbulence. Debris from the lysed cells
directly adjacent to the wire is flushed away
immediately. Cellular debris does not stick to
the wire and, even at the highest power settings,
only an extremely small quantity of heat is
generated and then quickly cooled so as not
to increase the temperature of the surrounding
environment.
PI-MC2010
MICRODISSECTION, MICROSURGERY
For surgery at the
micro level, such as
Xenopus Capping
Features
l 13 & 18 micron diameter wire cautery
tips with 1 & 1.5mm loop
l 15 Power settings
l Light weight pencil handle
l Foot switch activated
l Simple operation
Micro-Cautery Instrument
Comes with foot switch, pencil, tip, holder, power cord, operation manual,
and choice of one box of tips.
MICRO-CAUTERY ELECTRODE TIPS
504045
18 micron wire tip cautery electrode, red-black cover,10mm loop, 5/pkg
504046
18 micron wire tip cautery electrode, red cover,1mm loop, 5/pkg
504047
18 micron wire tip cautery electrode, red cover,1.5mm loop, 5/pkg
504048
13 micron wire tip cautery electrode, yellow,1mm loop, 5/pkg
504049
13 micron wire tip cautery electrode, yellow,1.5mm loop, 5/pkg
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
33
AN I MAL S U RGERY & S U PPORT
Small Animal Anesthesia
EZ-7000 Classic System
Components may not be as pictured.
EZ Anesthesia is the system of choice for anesthetizing small animals,
and it comes with a variety of choices. Animals to be anesthetized
are placed in the acrylic induction chamber and the system delivers a
precisely blended mixture of oxygen and isoflurane. An activated charcoal
air filter canister at the top of the chamber releases safe, filtered air back
into the room. A water-heated cage warmer or warming plate (ATC1000)
is used to retain the animal body temperature while in the induction
chamber. After the initial anesthetizing, the animal can be moved to the
heated surgical water bed and positioned properly in the snugly fitted
nosecone. A highly sensitive valve regulated by the animal’s breathing
works with the nosecone to ensure non-rebreathing efficiency, and allow
safe anesthesia for up to several hours.
The breathing device also includes an air filter that releases safe, filtered
air back into the room.
EZ Systems include all necessary hardware components and connections.
Oxygen and liquid Isoflurane are not supplied. These are required to
operate the system.
Each system comes with all necessary components and connections for
immediate use, including::
l Oxygen regulator
l Vaporizer Unit
l One Induction Chamber (standard EZ-178)
l One Breathing Device (standard EZ-103)
l Case of charcoal filters
Other available options include:
l Heated Beds and Heating Pump
l Additional Breathing Devices
EZ-FF9000 Fixed Flow System
Features
lIntegrated turnkey solution for small animal
anesthesia
lSafe for surgical personnel, 90% below OSHA
Isoflurane limit
lDesigned by veterinarians
lCompact and portable
The Fixed Flow System is our most advanced system and provides preset,
fixed flow rates with no need for adjustment. The system offers five gas
outlets, each with an individual ON/OFF switch. The system ensures
consistent, precise gas flow when connected to any pressurized gas source
higher than 7 psi.
l Induction Chamber output is fixed at 1 Lpm
l Four Breathing Circuit outputs are fixed at 0.5 Lpm
This unit features an oxygen flush button that purges the induction
chamber with pure oxygen, thereby protecting personnel from exposure
to anesthesia gas when opening the chamber. A handle on the vaporizer
makes the unit easy to safely transport.
EZ-FF9000 Fixed Flow System
lTime efficient and cost effective
EZ-7000 Classic System
lVirtually stress-free for the animals
lEasy to setup and use, simplifying the training of new
staff and reducing the threat of human error
lSpeedy recovery time
The Classic System is the upgraded version of our original EZ-Anesthesia
system. The new version has a reconfigured flow meter that is more
user-friendly. The system offers five gas outlets that can supply a single
induction chamber and four breather circuits simultaneously.
The upgraded system also features an oxygen flush button that purges the
induction chamber with pure oxygen, thereby protecting personnel from
exposure to anesthesia gas when opening the chamber. A handle on the
vaporizer makes the unit easy to safely transport.
EZ-7000
34
Classic System
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
EZ-B800 Basic System
EZ-177 and EZ-1785 Induction Chambers
The Basic System is designed for use with a single animal. It utilizes one
output directly from the vaporizer into a Y-splitter which creates a dual
feed that can be directed to the induction chamber or the breather circuit.
These chambers incorporate a positive seal O-ring gasket for containment
during use.
Sure-Seal Mouse Chamber (5"L × 4.75"W × 4.38"H) Sure Seal Mouse/Rat Chamber (9.75"L × 4.75"W × 4.38"H)
Large Mouse/Rat Induction Chamber (7"W × 11"D × 6"H inside)
Rabbit/Guinea Pig Induction Chamber (12"W × 18"D × 12"H inside)
This unit incorporates an oxygen flush system that purges the induction
chamber with pure oxygen, thereby eliminating personnel exposure to
anesthesia gas when opening the chamber.
EZ-177
EZ-178
EZ-1785
EZ-179
EZ-B800
Basic System
EZ-B800A Basic System without Regulator
EZ-320 and EZ-330 Oxygen Regulators
Regulators are preset to 50 psi.
EZ-1130 Connection Kit
l EZ-320 utilizes a CGA-540 connection for large “H” tanks.
This kit is used for connecting additional components to the EZ-Anesthesia
Systems. It includes 6 ft of clear PVC tubing with a quick-disconnect fitting.
EZ-1130
EZ-320
EZ-330
Connection Kit
EZ-830 Ventilator Connection Kit
This kit is used to connect the SAR-830 ventilators with EZ-Anesthesia
Systems. It includes all required components, pre-assembled for simple
connection between the ventilator and the anesthesia system.
Oxygen Regulator for large tanks
Oxygen Regulator for small tanks
EZ-340-XXXX Custom Hose Assemblies
Custom built hose assemblies for wall or ceiling outputs specific to facility
needs: Chemetron, Ohmeda, Schrader or DISS.
Ventilator Connection Kit
Euthanex Lids
The Euthanex Lid system has
become an industry standard.
You no longer need to transfer
animals. The lids are available
in five sizes to accommodate
virtually all plastic cage sizes
designed for small lab animals. These
heavy duty stainless steel lids have a stem
fitting for connection to the quick-disconnect fitting on the hose from
the gas source. A foam lid gasket ensures a good seal on the cage.
Multiple lids may be used to treat several cages at once.
EZ-20027 Small Lid (13” x 9”) Fits old-style mouse cages
EZ-20028 Small Lid (16” x 10”) Fits new-style mouse cages
EZ-20030 Square Lid (13” x 13”) Fits Thorn cages
EZ-20032 Medium Lid (20.5” x 11”) Fits rat cages
EZ-20034 Large Lid (23” x 16.5”) Fits guinea pig cages
EZ-20029 Lid Storage Bracket (wall-mounted,holds up to four lids)
EZ-20027G Small Lid Gasket (13”x9”)
EZ-20028G Small Lid Gasket (16”x9”)
EZ-20030G Square Lid Gasket (13”x13”)
EZ-20032G Medium Lid Gasket (20.5”x11)
EZ-20034G Large Lid Gasket (23”x16.5”)
Vaporizer Pole Mount
The EZ-E28000 is a mounting option for the
EZ‑Anesthesia system, combining system portability
with a small footprint. Constructed of Aluminum and
Stainless Steel, it features a five leg base for maximum
stability and an oxygen “E” tank mounting bracket.
Anesthesia system must be purchased separately.
EZ-211
EZ-212
EZ-103
E-28000
Vaporizer Pole Mount
Mobile Workstations
EZ-107
EZ-104A EZ-103A EZ-107A EZ-109 EZ-211 EZ-212 Versaflex Non-Rebreathing Unit
Microflex Non-Rebreathing Unit
Rat Stereotaxic Non-Rebreathing Unit
Multi-Animal Non-Rebreathing Unit
Mouse/Rat Thin-Line Heated Waterbed
Mouse/Rat Standard Heated Waterbed
Two mobile workstations, constructed of heavy-duty stainless steel with
locking casters, integrate all your EZ-Anesthesia components into one portable
unit. Open side shelves accommodate 20 lb. cylinders, and convenient 2" port
holes allow for easy rigging of gas and electrical lines. Below the work surface
of each mobile workstation is an open shelf and a locking cabinet. EZ-E25000
provides a 42"x24" work surface and holds up to four cylinders. EZ-E27000
has a 22"x21" work surface and holds up to two cylinders. These systems are
easy to set up and provide maximum flexibility and mobility.
E-25000
E-27000
Mobile Workstation, 42" x 24" Top
Mobile Workstation, 22" x 21" Top
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
35
AN I MAL S U RGERY & S U PPORT
EZ-830
l EZ-330 utilizes a CGA-870 connection for small “E” tanks.
AN I MAL S U RGERY & S U PPORT
Electronic von Frey Anesthesiometer
l Plug up to three probes into a single unit
l LCD readout (Floating or last maximum/minimum)
l Rigid tips up to 800 gm
l “Supertips™” 15 up to 65 gm
l 1,000 gm probe available
l Independence from temperature
l Optional analog output cable for chart recorder
l Pipette tips can be customized to any specification
l Microprocessor electronics 0.1 gm plug-in probes
II-2390
II-2391
II-2392
II-2393
II-23931
II-2394
II-2395
II-2396
II-2397
II-2400
Electronic von Frey Anesthesiometer, rigid tips, 90 gram range
Electronic von Frey Anesthesiometer, rigid tips, 800 gram range
Electronic von Frey Anesthesiometer, rigid & 15 super tips, 90 gram range
Electronic von Frey Anesthesiometer, rigid & 15 super tips, 800 gram range
Electronic von Frey Anesthesiometer, custom rigid tips, 1000 gram range
von Frey Probe, 90 gram range
von Frey Probe, 800 gram range
von Frey Probe, 1000 gram range
MRI Probe Option (add to price of probe above)
Analog Output Cable
To assess mechanical allodynia, which is a
painful response to a light touch or pressure
from a stimulus that is not normally painful,
the Electronic von Frey Anesthesiometer was
developed. The Electronic von Frey meter uses
one of 15 different flexible von Frey hairs called
“SuperTips™” (or rigid tips up to 800 grams).
Each hair, regardless of model chosen, is
exactly 0.8 mm in diameter. This uniformity of
design eliminates false readings and allows for
comparison of test results. The Electronic von Frey
can be used with chart recorders and analog/
digital converters, and it never needs calibrated.
This system includes either a 90, 800 or 1,000
gram probe. Mesh stands are available in a
variety of sizes for large group studies.
Trio
Get three test systems in one package with the Trio, featuring the
Electronic von Frey, Plethysmometer and Randall Selitto Meters. Just like
the Quattro package, the modular design allows these three test systems
to communicate with the same electronic controller. The stand and sling
for the Randall Selitto test are sold separately.
II-2888
Trio 3-in-1 System
Quattro
This special package offers four tests, including Electronic von Frey,
Plethysmometer, Randall Selitto and the Grip Strength Meter. You get all
four test modules and the electronic controller that is interchangeable
with all four systems. The electronic controller has up to three inputs,
so you can perform up to four unique tests with only one electronic
system. If you prefer, you may build your system and you grow. Because
of the modular design of these four systems, you need to order only
one complete system. Then, the modules for the other three tests, which
integrate into the system, can be purchased separately, as needed. The
stand and sling for the Randall Selitto test are sold separately.
II-2889
36
Quattro 4-in-1 System
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Plethysmometer (Paw Volume) Meter
l No wetting solution needed
l One calibration/year
l Battery-operated or line powered controller
l One-year warranty
Test the effectiveness of anti-inflammatory agents and agents to reduce
edemic conditions with the Plethysmometer. Simply insert the rat or mouse
paw into water. A sensor in the water notes a pressure change when the
paw is immersed. Pressure is calibrated in 0.1 milliliters and displays on the
battery-powered monitor. The acrylic stand offers full visibility of the subject
throughout the testing.
AN I MAL S U RGERY & S U PPORT
II-520MR Paw Volume Meter for Mouse & Rat
Randall Selitto Paw Pressure Meter
l Hands free operation with footswitch
l Visible force limit indicator
l Portable electronic display
l Battery-operated or line powered controller
l 3-oz. handheld probe with accuracy of 0.5%
l No calibration required
The Randall Selitto Paw Pressure Meter for
analgesia testing is digitally controlled. Use
the handheld instrument to apply force to an
animal’s extremity and get instantaneous, live
readings. You can even view the last maximum
force applied during the test. The new limit
II-2500
indicator lets you select the maximum force
limit, and then indicates with a warning light
when the system reaches that limit. This unit
comes with an acrylic stand to allow for easy
viewing of the display. Stand and sling are sold
separately.
Randall Selitto Paw Pressure Meter, 800 g pressure applicator
Grip Strength Meter
l Mouse and rat wire mesh grid plates included
l Maximum force range is 2,000 gm (10% over
range allowed). Higher ranges are available
by special order.
l 1 gram increment readings
l Suction feet on the heavy, anodized base plate
resist even large pulling forces
l Battery-operated or line powered controller
l One year warranty
Measure muscle hyperalgesia in rats and mice
with the Grip Strength Meter, which gauges
the forelimb grip force using a digital force
transducer. Simply hold the animal by the
tail and gently dangle it over the wire mesh
plate until the animal grasps the plate with its
forepaws. The force transducer, connected with
the wire mesh plate, measures the strength of
the animal at the time of the test. The batteryoperated, electronic control device calculates the
II-2200
average of three measurements, and it holds the
last maximum force in a “peak and hold” type
readout until you reset it.
Grip Strength Meter for Mouse & Rat
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
37
AN I MAL S U RGERY & S U PPORT
Incremental Hot Cold Plate
Analgesia Meter
l Heat or cool, from 0-70˚C
l Ramping temperatures for threshold and latency results
l Rapid increase or decrease in temperature
l Precise programmable digital control
l Printout of data
l Temperature stability is 0.1˚C
l Includes clear animal enclosure
l Plate size 4 x 8”
l Two-year warranty
This safe, humane device for rats and mice
is used for latency and threshold-based
nocicetption, ramping temperatures for 0-70˚C.
Because this hot cold plate is incremental, it
measures latencies of much more than just the
strong narcotic agents, broadening dramatically
the range of analgesia research with devices
of this type. Microprocessor-controlled, the
Incremental Hot Cold Plate can heat or cool
in increments of 0.1˚C, at a rate of 1-10˚C per
minute. With uniform heating and cooling
and upper/lower cut-off limits, this device is
II-PE34
predictable and safe. It can also function as a
constant temperature plate with great stability
(0.1˚C). As soon as a reaction is observed from
the chosen paw, the unit reverses to the standby
temperature.
Incremental Hot Cold Plate Analgesia Meter for Mouse & Rat
Incapacitance Meter for Mouse & Rat
l Precise programmable digital control
l Start, stop and reset test from controller’s
front panel
l 180-270 gram holder included (other
sizes available)
l Reaction detected automatically
l Manual override of all timer functions
l Alphanumeric readout
l 5 to 999 seconds test period
l Alphanumeric readout
l All functions and parameters entered via
key-pad
l Two-year warranty
II-600MR Incapacitance Meter for Mouse & Rat
38
Test pain and inflammation in the hind limbs of mice, rats or birds with
the Incapacitance Meter. It uses a technique called dual channel weight
averaging, which tests both hind limbs. This gives you a clean, stress-free
correlation of the paw pressure test. Conduct control and testing of the
animal at the same time. Place the animal in the holder with its hind limbs
resting on the two weight-averaging platform pads. The controller records
the average weight (grams) over the test period as the animal shifts its
weight from each pad
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Hot Plate Analgesia Meter
l Includes both mouse and rat enclosure
l Temperature indicated in 0.1˚C increments
l Holding accuracy is +/- 0.1˚C
l Digitally controlled
l Two-year warranty
II-39
Hot Plate Analgesia Meter for Mouse & Rat
Plantar Test Apparatus/Tail Flick Test Analgesia Meter
l Includes three acrylic animal enclosures
that each hold two rats or four mice
l Precise programmable digital control
l User-defined humane cutoff feature
l Adjustable beam intensity in 1%
increments up to 250˚C
l Reaction is detected automatically
This unit, which is designed for testing narcotics
and strong non-narcotic drugs, offers both
Plantar (Hargreaves Method) and Tail Flick
testing with a single unit. Either testing system is
also available individually. In plantar mode, the
visible light/heat source is directed at the paw or
other desired body part, and in tail flick mode
it is directed at the subjects’ tails. Test up to 12
mice or 6 rats simultaneously. If desired, other
animals like cats and rabbits may also be used.
Tests are simple to setup. The focused, radiant
heat/light source creates a 4 x 6 mm intense
spot. Because the light is visible, you know
when the test starts and ends. The equipment is
silent (no whining or clicking sounds) to avoid
l Alphanumeric readout
l Manual override of all timer functions
l All functions and parameters entered via
key‑pad
l Heated glass option
l Tail temperature monitor option (for use
with the Tail Flick meter)
triggering an automatic response in conditioned
animals. You can set a humane cutoff timer that
automatically shuts off the heat if no response is
observed during the designated time frame.
When an animal is placed on cold glass,
its reaction time may be slower. This unique
system offers a heated glass option that prevents
the glass enclosure from acting as a heat sink,
giving a more accurate reading. An optional tail
temperature monitor can also be selected for
use with the Tail Flick meter. This option actually
preheats the tail before experimentation. Once
the preset tail temperature is reached, the test
and timer automatically begin. A glass stand
is also available in two sizes for large group
studies.
II-336T
Combination Plantar/Tail Flick Meter, non-heated glass and tail
temperature for mouse and rat
II-336TG Combination Plantar/Tail Flick Meter, tail temperature and heated glass
for mouse and rat
II-390
Plantar Test Analgesia Meter, non-heated glass for mouse and rat
II-390G Plantar Test Analgesia Meter, heated glass for mouse and rat
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
39
AN I MAL S U RGERY & S U PPORT
Use the Hot Plate Analgesia meters for latency testing in rats and
mice. Simply place the animals on a black anodized, aluminum plate
(11 x 10.5 x 0.75”, 275 x 263 x 15 mm) and set the plate’s surface
temperature to the desired setpoint (up to 75˚C). The plate maintains a
consistent temperature throughout the test.
AN I MAL S U RGERY & S U PPORT
Animal
Temperature
Controller
ATC2000 is a low noise heating system
for maintaining animal body temperature
during experimental procedures. The
DC heater is extremely quiet in terms of
electromagnetic radiation. This is essential in
electrophysiological recordings which are very
sensitive to electromagnetic interference.
The controller uses proportional, integral, and
derivative (PID) technology in adjusting the DC
voltage output. Compared with switched on/off
type controllers, PID controllers provide a much
more precise and stable control of temperature.
The PID approach is also more immune to
the variation of the experimental conditions
such as change in animal size and unexpected
disturbances.
l PID control for maximum temperature stability
l Low Noise DC Heater
l Three temperature sensor inputs
l Automatic shutdown if the plate reaches 45°C
●●
The controller has three temperature sensing
inputs.
●●
●●
The resistive temperature device (RTD)
Probe input can be used to monitor an
RTD rectal probe to control the animal
temperature or to monitor ambient
temperature, induction chamber
temperature or any other temperature.
When using a thermocouple probe, the
thermocouple (TC) probe input can be
used just like the RTD input. (A T type
thermocouple must be used.)
The heater plate also has an internal
temperature sensor. The ATC2000
monitors this sensor to prevent the
localized hot spots under animal.
●●
The controller has three operational modes:
●●
Normal mode uses the configured sensor
(RTD or TC) or the plate sensor to drive the
control loop.
●●
Adaptive mode uses the temperature of
the heated plate and the temperature of
the subject to control. This approach is less
prone to overshoot, but somewhat slower
the normal mode, depending on the
sampling rate used.
Shutdown is a fail safe mode used if the
plate temperature ever exceeds 45°C.
The ATC2000 is tuned at the factory. However,
the PID parameters may also be set manually.
The temperature resolution of the controller is
0.1°C. The rectal temperature probe has a 6-ft
ultra-flexible, shielded cable and an RTD sensor.
The metal heating plates (available separately)
have built-in temperature sensors. Compatible
with stereotaxic systems, the rigid, flat surface
fits under the U-frame. Plates are washable with
water and detergent.
ATC2000 Specifications
Temperature Range . . . . . . . . . . . up to 45°C
Resolution . . . . . . . . . . . . . . . . . . . 0.1°C
Accuracy . . . . . . . . . . . . . . . . . . . . . ± 0.3°C
Rat Sensor . . . . . . . . . . . . . . . . . . . RTD, OD 2.0mm tube with 3.5mm ball head
(Optional mouse sensor is available)
Maximum DC Output . . . . . . . . . . 10V, 3A
Power . . . . . . . . . . . . . . . . . . . . . . . . Universal AC Adapter 90–264V Input,
[email protected] maximum output
An optional silicone pad (WPI #503573) is composed of a thermally
conductive rubber material that is used to enhance the heat transfer to the
animal. Simply place the silicone pad on the ATC2000 warming plate and
place the animal on silicone pad.
ATC2000 Animal Temperature Controller
REQUIRED Accessories (select one sensor and one plate)
502195 Small Heating Plate with built-in RTD sensor, 10 x 15 cm
502196 Medium Heating Plate with built-in RTD sensor, 15 x 25 cm
REPLACEMENTS
502197 Rectal Temperature Probe, 2 mm shaft diam., 3.5 mm ball tip
503524 Mouse Probe, 1.8 mm shaft diam., 2.5 mm ball tip
503567 Mouse Adaptor Heating Plate with built-in RTD sensor, 4x15 cm (for 502063 Stereotaxic Adaptor)
503573Silicone Pad
40
Dimensions . . . . . . . . . . . . . . . . . . . 45x30x7 cm (17.7 x 11.8 x 2.76 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . 5 kg (11 lb)
NEEDLE MICROPROBES (see page 51 for details)
MT-29/1
29 ga 1 cm Needle Microprobe, 5-ft cable
MT-23/3
23 ga 3 cm Needle Microprobe, 5-ft cable
MT-D
Needle Microprobe, 5-ft cable
FLEXIBLE IMPLANTABLE PROBES (see page 51 for details)
IT-18
0.025-inch diam Flexible Implantable Probe, 3-ft cable
IT-23
0.009-inch diam Flexible Implantable Probe, 3-ft cable
IT-1E
0.025-inch diam Flexible Implantable Probe, 3-ft cable
RECTAL PROBES (see page 51 for details)
RET-2Rectal Probe for Rats, 5-ft cable
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
CW-SAR1000
The CW-MRI-1 ventilator
is a small animal
ventilator designed for
use in MRI and other
highly magnetic field
environments, though
its use is not limited to
those environments. It
offers manual or remote
operation of pneumatic,
non-metalic valves. The
CW-MRI-1 operates on
the flow-time principle,
dispensing a known airflow into the lungs for a set inspiratory time to generate
desired tidal volumes. Three controls–respiratory rate, inspiratory time and
flow rate–allow for accuracy and extraordinary flexibility over a wide range of
volumes, breaths-per-minute and inspiration/expiration (I/E) ratios. The unit can
be expanded to ventilate larger animals. A source of compressed air or helium
is required for operating the pneumatic valves.
The CW-SAR1000 Small
Animal Ventilator is a versatile
and easy-to-use ventilator
for mice, rats, and other
small animals. Volume mode
delivers a fixed tidal volume
on each breath, and pressure
mode causes inflation to stop
at a preset airway pressure. In either mode, lung inflation is maintained at the
end of inspiration until the percent inspiration (%Insp) time has elapsed. This
end inspiratory pause (IEP) improves gas exchange and the overall efficiency
of ventilation. In volume mode, the ventilator operates on the proven flow-time
principle, where a known airflow is delivered for a set time, thus delivering a
fixed volume. In pressure mode, airflow is simply adjusted to give a suitable
inspiration time. Actual tidal volume is computed and displayedon the LCD
display, as well as minute ventilation (MV) and other respiratory parameters. A
built-in air pump allows completely self-contained operation. This pump draws
in room air, oxygen, or anesthetic gasses through a rear-panel port.
Features
l Non-metalic, pneumatic valves
l Wide tidal volume and rate range
l Safe with oxygen and anesthesia
l Internal air pump
l Direct readout of volume and rate
●●
●●
●●
●●
●●
●●
●●
CW-MRI-1
CW-SAR1000
Ventilator
Features
Pressure or volume-cycled operation
Wide tidal volume and rate range
User-friendly set-up and operation.
Direct display of Tidal Volume & all parameters
Remote control via USB port
Safe with oxygen and anesthesia
Multi-animal set-ups
Ventilator
VENTILATOR Specifications
CW-MRI-1
CW-SAR1000
RESPIRATORY RATE RANGE
5-150 breaths/min
5-200 breaths/min
TIDAL VOLUME RANGE
0.1-30 mL
0.2-35 mL (using internal valves. External valve
assemblies available for larger animals provide
up to 350 mL volumes.)
INSPIRATION/EXPIRATION RANGE
20-80%
INTERNAL AIR PUMP CAPACITY
4 LPM
INSPIRATORY FLOW RANGE
50-1000 mL/min
10-1000 mL/min
0-50.0 cmH2O
PRESSURE CONTROL RANGE
POWER
120/240V (switchable), 100VA
120/240VAC, selectable
DIMENSIONS
9x5.5x9 in. (23x14x23cm)
9x4x9 in. (23x10x23 cm)
microCAPSTAR CO2 Analyzer
Features
l
l
l
l
Use with Ventilated or unassisted animals
End-tidal peak or continuous CO2 readings
Low sample flow requirements, rapid response time
Simple one-gas calibration
CW-microCAPSTAR Specifications
microCAPSTAR extends CO2 monitoring to the realm of small animals.
The advanced features, reliability and ease of operation make it the
perfect companion to the CW-SAR-830/AP small animal ventilator.
Featuring low sample requirements, rapid response time and long-term
stability, this CO2 analyzer provides accurate end-tidal or continuous
measurement of expired CO2. The LCD screen displays CO2 concentration
(instantaneous ETCO2 in either percent or mmHg), respiratory rate
measurements and trend plot of the end-tidal values. An adjustable ETCO2
alarm warns when end-tidal values fall out of the user-adjustable preset
range. Built-in diagnostics warn of plugged sample tubing or other fault
conditions.
MEASUREMENT RANGE
0-9.9% (0-76mmHg) CO2
RESPONSE TIME
75mS at 70mL/min through cell
150mS at 50mL/min sampling
MAXIMUM SAMPLE CELL PRESSURE
+5PSIg
SIGNAL OUTPUTS
BNC
POWER
120VAC/220VAC switchable, 25VA
DIMENSIONS
19x5.25x16”, 49x13x41cm
WEIGHT
10lb. (4.5kg)
CW-MICROCAPSTAR
CO2 Analyzer
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
41
AN I MAL S U RGERY & S U PPORT
CW-MRI-1
AN I MAL S U RGERY & S U PPORT
Unique environmental control
system and single tail cuff allows tail
movement, minimizing stress in the
animal subjects
l Test up to 200 animals at a time
l Highly sensitive photoelectric sensor for
blood pressure detection
l Sensor is MRI compatible
l Quick and accurate blood pressure
measurement at temperatures as low as 32˚C
This revolutionary design brings non-invasive blood pressure testing to a
new level — a true turn-key system for accurate, consistent blood pressure
measurement on mice, rats or any other laboratory animal test subject.
It is a compact, simple yet versatile system that can test from one to
200 animals at a time with independent control of each channel. Simple
daisy-chaining allows expansion of up to 200 independently controlled
systems.
All components are built into one small unit — controls, inflation of tail
cuff, warming environment with whisper-quiet fans — providing an ideal
system for teaching facilities and for the pharmaceutical industry when
high throughput is a must.
Single animal systems are controlled from the touch screen, which allows
keying in all necessary test setups. Touch screen control allows ease
of operation, supplying automatic evaluation of test results — systolic,
diastolic, mean and heart rate.
42
l Monitor, record, store or export real time
systolic, diastolic, mean and heart rate
Data is collected, stored, displayed and can be transferred to the
supplied memory stick. The USB interface allows for software control of
multichannel systems. For single animal systems, built-in software lets you
view and export data. Reports are in an Excel-style format and may be
easily exported.
No computer is required. However, the analog output may be interfaced
with your own data acquisition software.
System is easily cleaned. Removable trays are included with each system.
In addition to the standard one-year warranty on the system, tail cuff
sensors have a lifetime warranty..
Includes all accessories necessary to run system.
II-MRBP-M Mouse Blood Pressure System
II-MRBP-R Rat Blood Pressure System
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Blood Pressure Monitor and Transducer
● Monitors animal arterial or venous blood pressure
● Displays systolic, diastolic or average blood pressure
BP1 accepts WPI’s BLPR blood pressure transducer (below) as well as other
blood pressure transducers.
An audio monitor provides a signal with variable pitch and am­pli­tude,
allowing you to hear changes in blood pressure. Digital LCD display pro­vides
average or peak signal values from 0 to 1999 mV. With an optional pressure
gauge (not provided — see PM015D, page 187), the user may cal­i­brate the
display to read mm Hg. Re­cord­er out­put con­nec­tor al­lows di­rect con­nec­tion to
a pen re­cord­er, os­cil­lo­scope or com­put­er via a data acquisition system.
Pressure Monitor (transducer & cable not included)
Specify line voltage
ACCESSORIES
BLPR2
NEW Blood Pressure Transducer & Cable
BPCABLE2 NEW Cable (12 ft) with DIN connector for BLPR2
503067
BLPR2 Transducer without cable
13024
Single Rack Mount Kit
13025
Dual Rack Mount Kit
3491
Extension Cable, 5 ft
500184
BNC-to-BNC Cable, 10 ft
14036-15 4-Way Luer Stopcock, Blue Tint (package of 15)
KZ1101
Micro Cannula, 3 inch
SYS-BP1
AMPLIFICATION
x1, x10, x100, variable (x5 to x1000)
BANDWIDTH, SMALL SIGNAL (-3 dB)
300 kHz (x1)
30 kHz (x10)
3 kHz (x100)
0.3 kHz (x1000)
OUTPUT VOLTAGE SWING
± 5 volts
MAXIMUM OUTPUT CURRENT
2 mA
INPUT IMPEDANCE, EACH INPUT
100 kΩ || 0.01 µF
TRANSDUCER APPLIED VOLTS
10 V nominal, varies with load. 25 mA,
maximum
DIGITAL PANEL METER
0 to 1999 mV (calibrated in mm of Hg
pressure when supplied with pressure gauge). Meter reads average value or ± peak (1 to 20 Hz).
POWER
95-135 V or 220-240 V, 50/60 Hz
DIMENSIONS
8.5 x 5.12 x 10 in. (21.6 x 13 x 25.44 cm)
SHIPPING WEIGHT
11 lb (5 kg)
Note: BLPR2 is intended for animal research only and may not be used
for human blood pressure measurement.
BLOOD PRESSURE
TRANSDUCER
BLPR2 SPECIFICATIONS
WORKING PRESSURE
-50 to + 300 mm Hg
OVERPRESSURE
-400 to +4000 mm Hg
EXCITATION VOLTAGE
1-10 VDC or RMS to 5 kHz
INPUT IMPEDANCE
350 Ω ±10%
OUTPUT IMPEDANCE
300 Ω ±10%
ISOLATION
< 5 µA leakage at 120 VAC
SENSITIVITY
5 µV/V/mm Hg
DYNAMIC RESPONSE
100 Hz
EIGHT HOUR DRIFT
1 mm Hg after 5 minute warm-up
MAXIMUM ERROR
Total combined effects of Sensitivity, Linearity, Hysteresis (at 25°C and 5 µV/V/mm Hg) do not exceed ±2% or 1 mm Hg, whichever is greater.
Stopcock #14036
included with BLPR2
ENVIRONMENTAL
Operating Temp. Range
+15°C to +40°C
Storage Temp. Range
-30°C to +60°C
Light Sensitivity
Not affected by light under 3000 ft/candles (32,000 lux)
SHIPPING WEIGHT
1 lb
BLPR2 can be used for the direct arterial and venous pressure mea­
sure­ment in animal blood vessels. Supplied sterile, BLPR2 is accurate,
linear and stable with temperature. May be sterilized cold with Cidex or a
similar bac­te­ri­cide.
BLPR2 is equipped with a twelve-foot cable and connector com­pat­i­ble
with WPI’s four-channel signal conditioning unit, TBM4M Transbridge,
and the single-channel BP1 blood pressure mon­i­tor. Cable has moistureresis­tant locking connector. A continuous, uniform lumen eliminates
places for bub­bles to form and lodge. The clear fluid path is easy to
inspect. Easy to mount — slotted transducer body accepts Velcro strap.
To facilitate setup and operation, a four-way stopcock that al­lows easy
filling, flushing, and zeroing of the transducer is in­clud­ed. Typically, the
stopcock is located between the trans­ducer and the animal catheter
where it can be used to quickly zero, flush, or de-bubble the transducer.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
43
AN I MAL S U RGERY & S U PPORT
BP1 SPECIFICATIONS
AN I MAL S U RGERY & S U PPORT
Precision
Stereotaxic
Instruments
for Small
Animals
● 5 mm linear movement per
revolution on each axis
● Absolute lock at 90º
(vertical)
● Entire frame is electrically
continuous, ideal for
Electrophysiology
● Accessories available for
use with a wide variety of
small animals
WPI’s Precision Stereotaxic Instrument is
built around the time-proven U-frame design
concept, providing stability, and adaptability
to most species. Precision alignment ensures
accurate placement of electrodes, micropipettes,
and other devices. It is ideal for researchers
in need of a versatile, reliable instrument for
stereotaxic procedures with small animals
Versatility of positioning
The manipulator arm controls medio-lateral
and vertical positioning via lead screws with 80
mm of travel. This allows the fastest positioning
possible, consistent with lining up the scales
easily at a given coordinate. The anteroposterior movement is controlled via a dovetail
slide movement, with 80 mm of travel possible
in each direction. A universal joint allows the
investigator to change the angle of the probe up
to 90° in either the antero-posterior or mediolateral planes. The locking mechanism will hold
any angle position without drift or creep. It also
provides an absolute lock at 90° vertical.
TAXIC-600 Stereotaxic Frame
with UltraMicroPump Injection System
Easily read scales
All scales are oriented to be read easily from
the open end of the “U”. This is the position
from which most scientists prefer to work. The
numerals on the scales are clear and easy to
read. Precise alignment with facing vernier
scales gives accurate resolution to 0.1 mm.
Convenient for electrophysiology
The entire Stereotaxic frame including the
dovetails, manipulator arms and base are
electrically continuous. Grounding of the
entire frame including the base plate can be
accomplished by connecting the provided
grounding stud to earth. This is ideal for
electrophysiological studies where the animal
and surrounding structures need to be
grounded to reduce electrical noise.
Above: 502600 Precision Stereotaxic Frame.
At right: 502603 Dual Manipulator Stereotaxic
Frame.
44
502600
502650
502603
502653
TAXIC-600
TAXIC-650
TAXIC-603
TAXIC-653
Precision Stereotaxic Frame with 18°Ear Bars
Precision Stereotaxic Frame with 45°Ear Bars
Dual Manipulator Stereotaxic Frame with 18°Ear Bars
Dual Manipulator Stereotaxic Frame with 45°Ear Bars
Stereotaxic Frame with 18°Ear Bars plus UMP3-1 System
Stereotaxic Frame with 45°Ear Bars plus UMP3-1 System
Dual Manipulator Stereotaxic Frame with 18°Ear Bars plus UMP3-1 System
Dual Manipulator Stereotaxic Frame with 45°Ear Bars plus UMP3-1 System
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Large LCD display
from sealed sensors
on each axis
Quiet, batterypowered operation
This new Digital Stereotaxic Frame features
sealed electronic sensors and an easy-to-read
LCD display with 10-micron resolution in all
three axes. A zeroing function aids in targeting
specific coordinates. The battery-powered
display is electronically quiet, making it useful
in electrophysiology experiments as well as
keeping the workbench tidy.
Features
l Adaptors available for use with rats, mice,
birds, cats, geckos, guinea pigs and other
species
l 80 mm of vertical, lateral and anteriorposterior travel
l 180° rotation and lock at any vertical angle
502300
502350
502303
502353
l 360° rotation and lock at any level angle
l Syringe pump, microcamera and drill can be
attached directly
l Dual-lead screws provide stable and
accurate movement
l Extended base plate 400mmX255mm
suitable for various animals
l Vertical lock and fixing knob are separated
to ensure accurate position at any angle
l Maintains accuracy and flexibility under
different temperatures
l The precisely-designed rotary knob and U
frame allow sufficient space for the anteriorposterior operation
l Zeroing function for targeting specific
coordinates
l Laser engraved scales and darkened rod
make numerals easy to read
l 10µm precision of manipulator in all
directions
l Curved design of nose clamp fixes the head
of the animals more securely
l Desktop display is separate from sensors,
making it easy to read
l Battery-powered sensors, without electronic
noise, are suitable for electrophysiology
experiment
Digital Stereotaxic Frame with 18°Ear Bars
Digital Stereotaxic Frame with 45°Ear Bars
Dual Manipulator Digital Stereotaxic Frame with 18°Ear Bars
Dual Manipulator Digital Stereotaxic Frame with 45°Ear Bars
able
l
i
a
av
3-1
Also U M P ump
with icroP ice.
aM
pr
Ultr all for
—c
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
45
AN I MAL S U RGERY & S U PPORT
Digital Stereotaxic Frame with LCD Display
Stereotaxic Accessories
AN I MAL S U RGERY & S U PPORT
Adaptors
502063 Mouse and Neonatal Rat Adaptor
502213 Platform, Gas Anesthesia, with mouse Mask (use with 502063)
502062 Mouse Adaptor
502204 Rat Adaptor with a pair of ear bars, 18º
502226 Cat/Monkey Adaptor for 502600 series
502238 Spinal Adaptor for Rat
502060 Guinea Pig Adaptor for 502600 series
502241 Dog/Monkey Adaptor for Parallel Rail Stereotaxic instruments, with a pair of ear bars, 18º
The WPI Mouse and Neonatal Rat Adaptor (502063) employs
light, Delrin® adjustable ear bars with tapered points on one
end and thumbscrew on the other to facilitate surgery on
mice and rat pups. Adjustable ear bars may be independently
adjusted in height to level the skull. Laser engraved scales
show the vertical positions of the ear bars. A tooth bar and
nose clamp secures the nose. A well in the thick aluminum
body may be filled with dry ice and alcohol for hypothermic
anesthesia of neonatal animals. The adaptor clamps securely
on the right side of the “U” frame of the stereotaxic instrument.
When gas anesthesia is needed, a gas mask (502213) may be
mounted on the adaptor.
502241
502063
502213
502238
502062
502204
46
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
502237
OmniDrill35 not included
— see page 29.
502245
502068
502070
502235
502056
502225
502242
AN I MAL S U RGERY & S U PPORT
Probe Holders
502210 Probe Holder with corner clamp, 0.3-1.5mm opening
502067 Probe Holder with side clamp, 0.3-3.5mm opening
502070 Cannula Holder, opens to 3.4mm
502068 Large Probe Holder, 6.5-13mm opening
502237 Extra Large Holder for OmniDrill35 Microdrill
502236 Microdialysis Probe Holder, 1.5mm hole
502244 Micrometer Adjustable Electrode Holder, 10μ resolution, 25mm travel, 0.3-1.5mm opening
502245 Manual Microsyringe Injection Holder, 10μ resolution, 25mm travel
502244
Ear Bars
502055 Ear Bars, Rat, 18º, (pair)
502056 Ear Bars, Rat, 45º, (pair)
502224 Ear Bars, Cat, 18º, (pair)
502225 Ear Bars, Cat, 45º, (pair)
502235 Ear Bars, Mouse, 60º. Non-rupture, (pair)
502242 Ear Bars, Rat, Hollow. 1.5mm hole for auditory stimulation
502054
502053
502243
Other ACCESSORIEs
502053 Mask, Gas Anesthesia, Mouse
502054 Mask, Gas Anesthesia, Rat
502201 V-Clamp, 10/32 screw
502213 Platform, Gas Anesthesia, with mouse Mask (use with 502063)
502243 Adjustable Stage Platform for 502600 series, 2cm high
503598 Micro-Drill, 35K RMP, 110/220VAC, w/ a set of bits
503599 Micro-Drill, 35K RMP, 240VAC, w/ a set of bits
503567 Heating Plate for 502063, 4X15cm, 5mm thick (use with ATC1000)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
47
l
i
a
R
l
t
e
n
l
l
e
a
m
r
Pa
stru
AN I MAL S U RGERY & S U PPORT
n
I
c
i
x
a
t
Stereo Animals
e
g
r
a
L
for
WPI’s Parallel Rail Stereotaxic Frame systems are heavy-duty research
instruments for large laboratory animals such as cats, monkeys, and dogs.
The solid large frame and superior rigidity ensure the precise alignment
of animals for Stereotaxic surgery, injection, and recording. The system
can adopt up to four manipulators with 100-micron resolution on each
axis. Each manipulator can smoothly moved to and locked at any location
on both parallel rails in an arrange of 20 cm.
Parallel Rail Stereotaxic Frame system for large animals include the
Parallel Rail Frame, base plate, manipulator(s), Cat/Monkey or Dog
adaptor, and swivel mount.
502227
502228
502229
502230
502231
502232
502233
502234
Stereotaxic Frame System with one manipulator for Cat and Monkey
Stereotaxic Frame System with two manipulators for Cat and Monkey
Stereotaxic Frame System with three manipulators for Cat and Monkey
Stereotaxic Frame System with four manipulators for Cat and Monkey
Stereotaxic Frame System with one manipulator for Dog
Stereotaxic Frame System with two manipulators for Dog
Stereotaxic Frame System with three manipulators for Dog
Stereotaxic Frame System with four manipulators for Dog
For more options and accessories, visit the website — www.wpiinc.com
48
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Neuroscience Cannulas
Dummy Cannula
The Dummy Cannula
has a stainless steel wire
core, and it is placed in the Guide
Cannula when the Internal Cannula is removed.
It seals the opening and prevents tissue from
entering the bottom of the Guide Cannula. The
Dummy Cannula is threaded to securely tighten
it so that the animal will not unscrew it while
grooming.
For in vivo investigation of rodents
Dummy Cannulas are ordered based on the
projection length, just like the Internal Cannulas.
This cannula system for neuroscience study
and pre-clinical research includes an entire
range of cannula options. The three primary
components include the Guide Cannula, the
Internal Cannula and the Dummy Cannula
(cap)
l WPI cannulas are beveled inside and out
and then polished to remove any burrs and
ensure that the inside diameter is perfectly
cylindrical. This limits undesired trauma to
tissue and ensures smooth operation.
l WPI offers exceptional quality at the best
prices.
Choices include 22, 24 and 26 gauge.
Order the Guide Cannulas based on the gauge
and length from the base and the Internal or
Dummy Cannulas based on the length of the
Guide Cannula and the projection from the
Guide Cannula tip.
Length–Guide Cannulas can be ordered in
a range of lengths from 3.4–8.5mm. Length
can be specified to 0.1mm, with a tolerance of
±0.07mm. For example, a GC24-60 is a 24ga.
cannula that is 6.0mm long.
Understanding Part Numbers
For other cannulas, the length is determined
by the desired projection length and the guide
cannula length. The projection can extend
beyond the tip of the Guide Cannula up to
1.0mm.
Cannula Type
GC22-37
Cannula Type
Gauge of Guide Cannula
Length of Cannula or Projection
DUMC Length = GC Length + Projection
INC Length = GC Length + Projection
GC–Guide Cannula
INC–Internal Cannula
DUMC–Dummy Cannula
For example, for a Dummy Cannula flush
with the end of a 26ga Guide Cannula that
is 6.0mm long, order a DUMC26-60. For an
inernal cannula that projects 0.5mm beyond
the 6.0mm Guide Cannula (6.5mm), order an
INC24-65.
Gauge–Choose the gauge of the Guide
Cannula that will be used, even if you are
specifying an Internal or Dummy Cannula.
l Quantities of each item are kept in stock so
you can order as needed.
Guide Cannulas
GC Length
DUMC
Length*
INC
Length**
Projection
Guide Cannula
The Guide Cannula is a surgical grade,
stainless steel tube that is implanted into a
rodent's skull and cemented into place using
dental cement and screws. It guides the
Internal Cannula to the specific injection site.
Internal Cannula
The Internal Cannula is inserted into the Guide
Cannula to sample or inject fluid.
Guide Cannula
Insert the Internal Cannula
Press the cannula in until it contacts the base
Dummy Cannula
Projection
Internal Cannula
* DUMC Length=GC Lenth + Projection. If the cap is screwed on too tightly, the projection will be longer than expected.
** INC Length=GC Lenth + Projection. Internal cannula mounts flush and does not screw into place.
Qty 1-9 Qty 10-19
Qty 20-49
Qty 50-99
Qty 100+
Flexible PE Tubing
504278
0.25mm ID, 0.5mm OD, 1mm long
504279
0.42mm ID, 0.85mm OD, 1mm long
504280
0.6 mm ID, 1.1mm OD, 1mm long
Qty 1-9 Qty 10-19
Qty 20-49
Qty 50-99
Qty 100+
Misc. Accessories
504281
Fixing Screw for connection of INC & GC
504282
Instant Cyanoacrylate Adhesive, 3g
504283
Instant Cyanoacrylate Adhesive, 20g
Qty 1-9 Qty 10-19
Qty 20-49
Qty 50-99
Qty 100+
Cannulas
GC22-X
INC22-X
DUMC22-X
GC24-X
INC24-X
DUMC24-X
GC26-X
INC26-X
DUMC26-X
Guide Cannula, 22 Gauge, X.0mm
Internal Cannula, 22 Gauge, X.0mm
Dummy Cap Cannula, 22 Gauge, X.0mm
Guide Cannula, 24 Gauge, X.0mm
Internal Cannula, 24 Gauge, X.0mm
Dummy Cap Cannula, 24 Gauge, X.0mm
Guide Cannula, 26 Gauge, X.0mm
Internal Cannula, 26 Gauge, X.0mm
Dummy Cap Cannula, 26 Gauge, X.0mm
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
49
AN I MAL S U RGERY & S U PPORT
Exceptional Quality
Best Prices
Rapid Order Response
Ordering
● Super Accuracy ● Fast Response
● Analog output signal ● Multiple inputs
● Differential Temperature Measurement
BAT-10
Stand sold
separately
AN I MAL S U RGERY & S U PPORT
BAT-12
A Microprobe Thermometer is the instrument of choice for biological and laboratory
temperature measurements. These thermometers are very versatile, providing
fast response, high accuracy and stability with digital display and analog signal
for connection to a computer or recorder. With the wide selection of probes, the
instruments can be used in almost any application.
BAT-12 This thermometer has a sealed construction making it water, dust and fume
resistant. The BAT-12 has a single microprobe input and a single range with the
same high accuracy as the BAT-10. Comes complete with carrying case.
The thermometers can be used with any “Type T” thermocouple. Select a
temperature microprobe on the following page for your specific application.
BAT-10 This is the most versatile thermometer available. The instrument has a wide
temperature range and fast response with most microprobes. The BAT-10 accuracy
is NIST traceable and in each of the two temperature ranges, the accuracy is the
same as the resolution. There are three microprobe inputs, 1 and 2 can be selected
as separate inputs while 2 and 3 will read the differential temperature measurement
between the two. The instrument has automatic warnings for low battery or faulty
probes on the digital display. The linearized analog output (LOP) signal allows ease
of connection to a data acquisition system or recorder.
BAT-10R/LOP
Multiple Input Type T Thermocouple Thermometer, rechargeable NiCad batteries and 110 VAC adapter (microprobes ordered separately)
BAT-10R/LOP220 Multiple Input Type T Thermocouple Thermometer, rechargeable NiCad batteries and 220 VAC adapter (microprobes ordered separately)
BAT-12R
Single Input Type T Thermocouple Thermometer, rechargeable NiCad batteries and 110 VAC adapter (microprobes ordered separately)
BAT-12R-220
Single Input Type T Thermocouple Thermometer, rechargeable NiCad batteries and 220 VAC adapter (microprobes ordered separately)
OPTIONAL ACCESSORIES
EXT-6
Probe Extension Lead, 180 cm long
SBT-5
Thermocouple Switch Box, 5 probe inputs on back with numbered push button switches on the front. Connects 5 microprobes to one BAT-12R
501608
Tripod Stand for BAT-12
MICROPROBE THERMOMETER SPECIFICATIONS
BAT-10
Temperature Range
& Resolution
-200°C to +400ºC, 1ºC resolution
-100°C to +199.9ºC, 0.1ºC resolution
-100°C to +199.9ºC, 0.1ºC resolution
BAT-12
Differential Temp. Range
-19.99°C to +19.99ºC
N/A
Linearization centered at 40°C
0.01°C resolution
ACCURACY
1° Range
1°C ±1 least significant digit
0.1°C ± 1 digit between 0-50°C
0.1°C ± 1 least significant digit
0.1% ± 1 digit over full range
0.1° Range
Diff. Range
0.01°C ± 1 least significant digit
REPEATABILITY
50
± 1 least significant digit
Calibration CONFORMITY
Conforms to NIST tables
Follows NIST thermocouple tables
within 1 digit
Display31⁄2 Digit LCD
31⁄2 Digit LCD
INPUT SOCKET
Miniature, quick disconnect, copper-constantan Miniature, quick disconnect, copper-constantan
Analog output
Non-linearized set at 1.6 V, corresponding to temperature of 401°C
≈ 10 mV per degree C
POWER SUPPLY / BATTERIES
BAT-10: 4 alkaline “C” cells (life: 1000 hr)
BAT-10R: 4 Ni-Cad “C” cells (rechargeable unit)
BAT-12: 9V cell
BAT-12R: 9V Ni-Cad with charger
SensorS
Three Type T thermocouple inputs
One Type T thermocouple input
AMBIENT OPERATING RANGE
15-45°C
Auto-compensated to 0.1°C from 0°C to 50°C
dimensions
21.6 x 22.9 x 8.9 cm (8.5 x 9 x 3.5 in.)
12.7 x 6.4 x 15.2 cm (5 x 2.5 x 6 in.)
WEIGHT
1.6 kg (3.5 lb), including carrying case
1 kg (2 lb), including carrying case
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Temperature Probes
● Flexible Teflon microprobes are used for implantation in tissue,
in spectrophotometer cuvettes, rectally in neonatal mice, in water
baths, PCR thermal cyclers, etc.
● Animal rectal temperatures during surgical procedures and pyrogen
testing.
● Skin temperature measurement during exercise physiology studies.
Probe Type
Size
Style
Time
Isolated
Constant
an accuracy that is usually to 0.5°C or worse.
Furthermore, all our type T clinical probes
are guaranteed accurate to 0.1°C, due to our
stringent wire standards. These are five times
more accurate than competitive probes made
with regular “Special Limits” wire.
Max.
Lead
Temp.Length
AN I MAL S U RGERY & S U PPORT
When precise temperature measurements
are required, WPI can provide you with a
very accurate monitor and thermocouple
microprobes. WPI monitors have both resolution
and accuracy of 0.1°C in the 0-50°C range
and are traceable to NIST standards, whereas,
other competitive electronic thermometers have
Description
NEEDLE MICROPROBES
Fast-response needle probes for instant readings in tissue, semisolids, liquids, very small specimens, powders and materials. Needle tip is sealed to ensure only stainless steel contacts specimen.
MT-29/1
29 ga / 1 cm
A
0.125 sec
No
200°c
5 ft
29 gauge approximately 0.013-in
MT-29/2
29 ga / 2 cm
A
0.125 sec
No
200°c
5 ft
MT-29/3
29 ga / 3 cm
A
0.125 sec
No
200°c
5 ft
MT-29/5
29 ga / 5 cm
A
0.125 sec
No
200°c
5 ft
MT-26/2
26 ga / 2 cm
A
0.1 sec
No
200°c
5 ft
26 gauge approximately 0.018-in
MT-26/4
26 ga / 4 cm
A
0.1 sec
No
200°c
5 ft
MT-26/6
26 ga / 6 cm
A
0.1 sec
No
200°c
5 ft
MT-23/3
23 ga / 3 cm
A
0.15 sec
No
200°c
5 ft
23 gauge approximately 0.125-in
MT-23/5
23 ga / 5 cm
A
0.15 sec
No
200°c
5 ft
MT-23/8
23 ga / 8 cm
A
0.15 sec
No
200°c
5 ft
MT-4
29 ga / 1 cm
A
0.025 sec
No
200°c
5 ft
Similar to MT-29/1 but has a blunt tip. Good for instant skin and surface temperatures, liquids
MT-D
—
C
0.025 sec
No
200°c
5 ft
Fast response surface probe (stainless steel for locating inflammation, arteries, etc. Also for dental use.
FLEXIBLE IMPLANTABLE PROBES
Designed for high accuracy on extremely small specimens such as insects, seeds, etc. Maximum insertion depth 1/8". Totally sheathed in chemical resistant Teflon.
Sensor
Lead Diameter
IT-14
0.050" dia
D
0.3 sec
Yes
150°c
3 ft
0.025" dia
D
0.1 sec
Yes
150°c
3 ft
IT-18
IT-18EXLONG0.025” dia.
D
-
Yes
150°c
5 ft
IT-21
0.016" dia
D
0.08 sec
Yes
150°c
1 ft
IT-23
0.009" dia
E
0.005 sec
Yes
150°c
3 ft
IT-1E
0.025" dia
F
0.005 sec
Yes
150°c
3 ft
For ultra fast measurements and for use on micro-size specimens. Tissue implantable with 239a. Needle (supplied). Rather fragile. Teflon coated.
As IT-18 sensor except bead exposed. Combines ultra-
fast reponse of IT-23 with sheath strength of IT-18.
RECTAL PROBES
RET-2
-
G
0.8 sec
No
125°c
5 ft
RET-3
-
G
0.5 sec
No
125°c
5 ft
Rectal probe for rats typically for fast intermittent measurements. Smooth ball tip (0.125-in. dia.) with 1" long (0.59-in. dia) stainless steel shaft.
Rectal probe for mice similar to RET-2 except tip diam. 0.063-in. and shaft 3/4-in. long (0.028-in. diam.)
GENERAL PURPOSE
HT-1
H
0.5 sec
No
400°c
5 ft
HT-2
H
0.5 sec
No
400°c
5 ft
I
0.15 sec
No
240°c
5 ft
BT-1
“Workhorse” probe for liquids, gases, semi-solids. Plastic handle with straight stainless steel shaft. Not good for surface temperatures.
Like HT-1 except shaft length is 9-in.
Plastic handle with welded stainless steel, immersible shaft used for surface temperatures of solids, liquids, gases and semisolids. Tip is 0.02-in. diam., at right angle to probe to facilitate surface measurement.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
51
Intrathecal Catheters
504106
504107
504108
504109
Vascular & Arterial Catheters for Mice
504143
Mouse 32G Carotid Artery Catheter
504144
Mouse 32G Femoral Artery Catheter
504145
Mouse 32G Jugular Vein Catheter
504146
Mouse Catheter
CR3212 Cth Rsr 32G 12 w/ Stylet
CR3218 Cth Rsr 32G 18CM
CS1 32G Intrathecal Cth Sys ST
CS2 32G Intrathecal Cth Sys
AN I MAL S U RGERY & S U PPORT
Intrathecal Catheters for use with Osmotic Pumps
504110
32G Cth 10cm w/50cm Extension
504111
32G Intrathecal Cth w/PE50
504112
32G Intrathecal Cth w/Extension
504113
32G Cth CE w/3 Side Ports
504114
32G Intrathecal Cth w/Bead
504115
32G Intrathecal Cth w/Dacron Mesh & Stylet
504116
32G Intrathecal Cth w/Bead 185mm
Tail Vein Catheters
504147
Tail Vein Cth for Mouse 29G Cannula
504148
Tail Vein Cth for Rat 23G Cannula
504149
Angio Catheter for a Mouse
504150
DSA Catheter
504151
Rat Y Connector
Vascular & Arterial Catheters for Rats
504117
Jugular Vein Catheter 163mm (6 Pack)
504118
Femoral Artery Catheter 255mm (6 Pack
504119
Femoral Vein Catheter 190mm (6 Pack)
504120
Bile Duct Catheter 298mm (6 Pack)
504121
Carotid Artery Catheter 165mm (6 Pack)
ing
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The PI-MC2010 is a micro-cauterizing unit that embraces
thermodynamic technology to perform micro-surgical tasks
with efficiency and cleanliness. Cells just beyond the cutting
line are left healthy and functional.
How It Works
A current passes through the platinum cutting wire and
super-heats it at a preprogrammed rate for the span of a few
microseconds, then abruptly stops. A thin, gaseous-tube of
solution surrounding the wire is created because the rate
of heating is much faster than the velocity of the solution
molecules due to their average thermal energy at room
temperature. This creates an intense but finite shockwave as
the superheated tube of vapor expands from the wire inducing
turbulence. The quick heating and cooling of the platinum wire
causes a flushing effect as cool water rushes in after the brief
shockwave and turbulence. Debris from the lysed cells directly
adjacent to the wire is flushed away immediately. Cellular
debris does not stick to the wire and, even at the highest
power settings, only an extremely small quantity of heat is
generated and then quickly cooled so as not to increase the
temperature of the surrounding environment.
Features
PI-MC2010
l 13 & 18 micron diameter wire cautery
tips with 1 & 1.5mm loop
Comes with foot switch, pencil, tip, holder, power cord, operation manual,
and choice of one box of tips.
MICRO-CAUTERY ELECTRODE TIPS
504045
18 micron wire tip cautery electrode, red-black cover,10mm loop, 5/pkg
504046
18 micron wire tip cautery electrode, red cover,1mm loop, 5/pkg
504047
18 micron wire tip cautery electrode, red cover,1.5mm loop, 5/pkg
504048
13 micron wire tip cautery electrode, yellow,1mm loop, 5/pkg
504049
13 micron wire tip cautery electrode, yellow,1.5mm loop, 5/pkg
l 15 Power settings
l Light weight pencil handle
l Foot switch activated
l Simple operation
52
Micro-Cautery Instrument
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Macro sensors
Species
Nitric Oxide
Hydrogen
Peroxide
Oxygen
Hydrogen Sulfide
Order Number
Iso-Nop
ISO-HPO-2
ISO-OXY-2
OXELP
ISO-H2S-2
2 mm
2 mm
2 mm
2 mm
Price
Available Diameters
Response Time
< 5 sec
< 5 sec
< 10 sec
< 5 sec
Detection Limit/Range
1 NM to 80µM
< 100nM to 100µM
0.1%-100%
< 5nM-100µM
Sensitivity
≤2 pA/nM
<1pA/min
Yes
NaNO2 (10-6 or better)
#5436
#7325
#5435
8 pA/µM
0.3-0.6nA/%
2 pA/nM
0.1pA/min
< 1%/min
Yes
Yes
None
None
None
#600012
#100042
#600011
#5378
#7326
#600016
#100084
#5377
#600015
Drift
Temperature Dependent
Physiological Interference
Replacement Sleeves (pkg of 4)
Filling Solution
Start-up Kit
$
Yes
Micro Sensors
Species
Nitric Oxide
Price
(pkg of 2)
(pkg of 3)
(pkg of 2)
(pkg of 2)
Fiber Diameter (µm)
200
200
100
70
Tip Length2 (mm)
1-51
1-101
1-51
Response Time (sec.)
<5
<5
Lowest Detection Limit/
Range (nM)
0.2
Nominal Sensitivity-New
Sensor2 (pA/nM)
Baseline Drift (pA/min)
H202
(pkg of 3)
(pkg of 3)
ISO-H2S-100
ISO-HPO-100
ISO-NOPNM
ISO-NOP007
ISO-NOP30-L3
ISO-NOP3020
ISO-NOP3005
ISO-NOPF500-Cxx
ISO-NOP70-Lxx3
ISO-NOPF100
ISO-NOPF100-L3
ISO-NOPF200-Lxx3
ISO-HPO-100
ISO-NOPF200
Order Number
BIOS EN S I NG
nsor Body
mm long
Sensors
H2S
(pkg of 3)
(pkg of 3)
500
30
30
30
7
Conical tip:
100
100
3
5–10
0.5
2
3
2
2
1–41
2-51
<5
<3
< 10
<3
<3
<3
<3
<3
<5
~5
0.2
0.2
1
0.2
1
1
1
0.5
0.5
1
<5
≥20
≥50
≥10
≥10
≥ 20
≥1
≥1.5
≥1
≥1
≥0.5
≥1
1-4
none
none
none
none
none
none
none
none
none
none
<2.0
<2
7
100nm
(pkg of 3)
1Sensor
available in 1mm length increments (for example, 1mm, 2mm, 3mm...).
2Sensor sensitivity varies with length and diameter.
Any 10µm sensor can be purchased with a hypodermic sheath. Add a -H to the end of the part number (for example, ISO-HPO-100-H).
3L-shaped sensor for use with a tissue bath.
Some nitric oxide sensors are available in custom lengths. When ordering custom lengths, use the part numbers ISO-NOPF100-Cxx or ISO-NOPF200Cxx and replace the xx with the desired length. For example, for a 1mm flexible sensor tip, the part number should be ISO-NOPF200-C01. Sensors can
be ordered in the following custom lengths: 1mm, 2mm, 3mm, 4mm or 5mm.
Sensor Body
60mm x < 2mm∅
Ag/AgCl
Reference Electrode
Glass tip tapers to
~100µm Tip
~2mm
7µm Ø Fiber
Sensing Element
tapers to
~100nm tip
Ag/AgCl
Sensor Body
Ag/AgCl
60mm x <2mm∅
flexible
100, 200
or 500µm
2mm
or
0.5mm
5mm
~100–
200µm
2mm
NOPNM
ISO-NOPF
7µm
or
30µm
2mm
ISO-NOP007, ISO-NOP30
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
53
Nitric Oxide Sensor Guide
All WPI NO sensors are 100% compatible with ISO-NO Mark II (NOMK2), APOLLO 4000,
APOLLO 1000, and TBR4100 Free Radical Analyzer.
APPLICATIONS
in vivo
Cell Cultures,
NO 2- , NO3- ,
Tissue Bath
Tissue Bath
Microvessels
Microvessels
Single Cell
ISO-NOPF
ISO-NOP
ISO-NOP30L
ISO-NOP70L
ISO-NOP30
ISO-NOP007
ISO-NOPNM
100, 200, or 500 µm
2 mm
30 micron
70 micron
30 micron
7 micron
100 nm
RESPONSE TIME (with NOMK2)
< 5 sec
< 5 sec
< 3 sec
< 3 sec
< 3 sec
< 3 sec
< 3 sec
LOWEST DETECTION LIMIT
0.2 nM
1 nM
1 nM
1 nM
1 nM
0.5 nM
0.5 nM
TEMPERATURE SENSITIVITY
some
yes
yes
yes
yes
yes
some
DRIFT
none
none
none
none
none
none
none
10 pA/nM
2 pA/nM
1.4 pA/nM
1.4 pA/nM
1.4 pA/nM
1 pA/nM
0.5 pA/nM
none
none
none
none
none
none
none
SENSOR
SENSOR DIAMETER
SENSITIVITY
PHYSIOLOGICAL INTERFERENCE
Selectivity of WPI’s NO sensors
ISO-NOP – The original nitric
NO–3 and NO–2 Detection
A simple change in experimental protocol will
enable the ISO-NOP to be conveniently used
for indirect rapid accurate determination of
nitrite (NO –2) and nitrate (NO –3) concentration in
samples. Using this method a detection limit
for NO –2 or NO –3 as low as 1 nM is routinely
possible.
oxide probe – ideal for cell cultures, cell
suspensions and many other applications
The ISO-NOP is a popular, robust and high
performance sensor encased within a 2 mm
diameter disposable stainless steel protective
sleeve. The tip of the sleeve is covered with
a NO-selective membrane. Replacement
membrane sleeves can be purchased
separately (WPI #5436) and require an internal
electrolyte (WPI #7325).
Abdominal X-ray showing the appratus consisting of two customized ISO-NOP nitric
oxide probes, 4-channel pH catheter, and
Teflon nasogastric tube. (Courtesy Prof. K.E.L.
McColl, University Department of Medicine
and Therapeutics, Western Infirmary, Glasgow,
Scotland.) Iijima, K., et al. Gastroenterology
2002: 122: 1248-1257.
ISO-NOP
5435
5436
7325
7521
5399
7357
Replacement 2 mm shielded sensor and cable
ISONOP Startup Kit (recommended with first purchase)
Replacement Sleeve Kit for 2 mm sensor, pkg of 4
ISO-NO Electrolyte (10 mL)
ISO-NO Electrolyte, CO2-insensitive (10 mL)
T-Adapter Kit (pkg of 3) for ISO-NOP
Nitrite Standard Solution, 1 gram/liter (100mL)
ISO-NOPNM
The ISO-NOPNM NanoSensor has a tip
diameter of just 100 nm (0.1 µm) and a
detection limit for NO of less than 0.5 nM —
54
ISO-NOPNM
91580
SNAP50
800
Current / pA
making it indisputably the smallest and most
sensitive NO sensor in the world!
The ISO-NOPNM is based on a novel design
in which an electrochemically “activated”
Ag/AgCl
composite graphite nanofibre is used as
Reference Electrode
Sensor Body
70mm long
Glass tip tapers to
7µm
Fiber
theØNO-sensing
element. The surface of the
~100µm Tip
Sensing Element
NanoSensor is then modified using a unique
tapers to
multi-layered
NO-selective membrane.
~100nm
tip
Figure at right illustrates the response of the
ISO-NOPNM following successive additions
of nanomolar concentrations of NO. The
~100–
~2mm
200µm
ultra-low noise of the ISO-NOPNM (0.5 pA)
enables a detection limit of just 0.5 nM NO.
Schematic drawing of the new integrated NO nanoThe response time of ISO-NOPNM is less than
sensor. (US Patent Pending)
3 seconds.
– The world’s
smallest nitric oxide NanoSensor, designed
for measurement of NO at the cellular level.
Current / pA
BIOS EN S I NG
The ideal NO sensor should be insensitive to other reactive species likely to
be present within the measurement environment. The conventional Nafion
coated carbon fiber NO sensor exhibits a large response to such species.
WPI’s unique NO sensor technology utilizes a novel surface membrane
which amplifies the response to NO while eliminating responses to a vast
range of reactive species, including nitrite, absorbic acid, hydrogen peroxide,
catecolamines, and much more.
y = 0.5968x + 6.4967
R2 = 0.9973
600
400
200
0
0
300
600
900
1200
Concentration / nM
Time / S
Amperometric response of the NO nanosensor (ISONOPNM) to the successive additions of 2nM, 4 nM, 8
nM NO into 0.1 M PBS (pH=7.4).
100 nm NanoSensor, pkg of 3 (requires cable #91580)
Microsensor Adapter Cable
SNAP, 50 mg vial
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
ISO-NOPF
ISO-NOPF electrodes are the newest addition
to WPI’s nitric oxide sensor family and are
available in 100 µm and 200 µm diameters.
Utlilzing the latest advances in nanotechnology and material science, scientists at
WPI’s Sensor Laboratory have created these
completely flexible and virtually unbreakable
NO sensors. The new sensors are based on
a composite graphite NO-sensing element
combined with a reference electrode. The
surface of the sensor is then coated with a
unique multi-layered NO-selective membrane.
Sensor Body
60mm x < 2mm∅
50 pA
Current (pA)
– unique flexible NO
sensor! Designed for arteries, microvessels,
in vivo applications, and similar
applications.
flexible
100, 200
or 500µm
4 nM
2 nM
5mm
1 nM
2mm
Time (s)
Schematic drawing of ISO-NOPF.
Response of ISO-NOPF to NO.
ISO-NOPF100
ISO-NOPF200
ISO-NOPF500
91580
SNAP50
100 µm Flexible NO Sensor, pkg of 2
200 µm Flexible NO Sensor, pkg of 2
500 µm Flexible NO Sensor, pkg of 2
Microsensor Adapter Cable
SNAP, 50 mg vial
ISO-NOP007
ISO-NOP30 – 7 and 30 micron sensors with exceptional
7 µm
or
30 µm
20 sec
1600
Current / pA
Sensor Body
Ag/AgCl
60 mm x < 2 mm diam.
300 pA
Current (pA)
The ISO-NOP007 and ISO-NOP30 have recently been improved in
design and performance. The ISO-NOP007 has a tip diameter of just 7
microns and a length of 2 mm. The ISO-NOP30 has a tip diameter of 30
y = 1.3583x + 16.15
R2 = 0.9985
1200
800
400
0
0
300
600
900
1200
Concentration / nM
Time (s)
2 mm
The response of a 7 µm NO sensor (ISO-NOP007)
to successive additions of NO (100 nM). Inset shows
the linearity of the resulting calibration plot.
Schematic drawing of ISO-NOP007 and ISONOP30.
ISO-NOP007
ISO-NOP3020
ISO-NOP3005
91580
SNAP50
7 µm Nitric Oxide Sensor (pkg of 3)
30 µm Sensor Tips (2 mm length), pkg of 3 (requires #91580)
30 µm Sensor Tips (0.5 mm fiber), pkg of 3 (requires #91580)
Microsensor Adapter Cable
SNAP, 50 mg vial
Simultaneous measurement of force (top trace) and
changes of NO concentration (lower trace) in (A)
the rat superior mesenteric artery relaxed with ACh
and (B) a small human artery relaxed with ACh and
SNAP. In this artery oxyhaemoglobin(oxyHb) partly
reversed the increase in NO concentration, with
only a small change in force. [U. Simonsen, et al., J.
Physiol., 1999, 516: 271-282.]
Glucose Sensor Specifications
IGS100 – Implantable glucose sensor
Measuring glucose in vivo over the long term is challenging and difficult.
Previous measurement systems were limited to acute studies or a
few days at best. WPI introduces a new kind of implantable glucose
sensor based on a patented technology. This sensor provides a tool for
researchers to directly detect glucose in chronic studies in vitro or in vivo.
The sensor is fully compatible with WPI’s Apollo system.
IGS100
91580
Implantable Glucose Sensor (pkg of 2)
Microsensor Adapter Cable
In Vitro Precision
Coefficient of Variation (CV) ≤5%
Glucose Range
36 - 450 mg/dl (or 2-25 mM/L)
Response Time (sec)
100 - 300s
In vivo calibration
In vivo calibration
Interference species
Acetaminophen, ascorbic acid, uric acid
Length
5 cm
Sensor Size
0.6 × 0.7 mm
Reference ElectrodeAg/AgCl
Polarization voltage (V)
0.65 -0.7V vs. Ag/AgCl
Sensor Life
3-4 months in solutions at room temperature under continuous polarization; 15-30 days in vivo
Shelf Life
6 months
Operation Conditions
20° to 40° C (68° to 104° F)
Storage Conditions
10° to 25° C (50° to 77° F)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
55
BIOS EN S I NG
microns and is available in two different tip lengths (i.e., ISO-NOP3020
has tip length of 2 mm, ISO-NO3005 has tip length of 0.5 mm). The
response of the ISO-NOP007 and ISO-NOP30 is linear over a wide
dynamic concentration range of NO. The design of both electrodes
is based on a single carbon fiber coated with WPI’s NO-selective
membrane. A detection limit of approximately 1 nM NO makes these
electrodes ideal for use in tissues and microvessels.
performance — ideal for tissues and microvessels
2 mm
or
0.5 mm
Ag/AgCl
8 nM
10 sec
Hydrogen Peroxide & Oxygen Sensor Guide
ISO-HPO-2
ISO-HPO-100 ISO-HPO-100-LISO-OXY-2
APPLICATION
Cell Cultures, etc.
Tissue/Microvessels Tissue Bath
Cell Cultures, etc.
SENSOR DIAMETER
2.0 mm
100 micron
100 micron
2.0 mm
RESPONSE TIME
<5 SEC (90%)
<5 SEC (90%)
<5 SEC (90%)
<10 SEC (90%)
DETECTION LIMIT
<100 nM to 100 mM
1 nM to 1 mM
1 nM to 1 mM
0.1 % to 100%
DRIFT
<0.1 pA/min
<1.0 pA/min
<1.0 pA/min
<1%/min
SENSITIVITY
8 pA/µM
1 pA/nM
1 pA/nM
N/A
PHYSIOLOGICAL INTERFERENCE
none
Contact WPI
Contact WPI
none
BIOS EN S I NG
Hydrogen Peroxide Sensors
Hydrogen Peroxide is produced in biological systems by controlled
pathways at low concentrations that impact on cell signaling. At higher
concentrations inflammatory cells produce local intense amounts
of this oxidant to kill pathogens. In the progress of human disease,
uncontrolled formation of hydrogen peroxide from the mitochondrial
respiratory chain and enzymes, such as xanthine oxidase, can occur
(Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication).
Despite the recognized importance of this oxidant in biology real-time
measurements at low concentration have been difficult. The hydrogen
peroxide sensors developed by WPI are designed to compliment
existing high sensitivity fluorescent approaches with direct quantitative
measurement in biological samples in the low nM range.
Two hydrogen peroxide sensors are currently available. The ISOHPO-2 is a 2.0 mm stainless steel sensor, with replaceable
membrane sleeves (#600012) and an internal refillable
electrolyte (#100042). The sensor is designed for
use in cell cultures and similar
applications.
ISO-HPO-2
ISO-NOP30L
ISO-NOP70L
ISO-HPO-100-L
ISO-NOPF200-L10
ISO-HPO-100
Both sensors incorporate WPI’s proprietary combination electrode
technology whereby the hydrogen peroxide sensing element and
separate reference electrode are encased within a single Faradayshielded probe design. This design has been shown to enhance
performance during measurements and minimizes overall sensor size.
600011
ISO-HPO-2
ISO-HPO-100
600012
100042
Micrometer
Connect to
Microsensor
Cable #15810
Held in micromanipulator
The ISO-OXY-2 is a 2.0 mm stainless steel sensor, with replaceable
membrane sleeves (#5378) and an internal refillable electrolyte
(#7326). The sensor is similar in design to WPI’s popular OXELP
oxygen sensor (see page 68).
ISO-OXY-2
5377
5378
7326
2 mm Shielded Oxygen Sensor & Cable
ISO-OXY Startup Kit (recommended with first purchase)
Replacement Electrode Sleeve Kit, pkg of 4
ISO2 Filling Solution (electrolyte)
3 mm
carbon fiber
10 mm
L-shaped NO sensor used with
Myobath.
ISO-NOP30-L
NO Sensor, L-Shaped 30-micron (pkg of 3)
ISO-NOP-70-L
NO Sensor, L-Shaped 70-micron (pkg of 2)
ISO-NOPF200-L10 NO Sensor, 200 µm Flexible L-shaped (pkg of 2)
ISO-HPO-100-L HPO Sensor, L-Shaped 100-micron (pkg of 2)
91580
Microsensor Adapter Cable
56
ISO-HPO Startup Kit (recommended with first purchase)
2mm Shielded HPO Sensor & Cable
100 µm HPO Sensor (requires #91580), pkg of 3
Replacement Sleeve Kit for ISO-HPO-2, pkg of 4
ISO-HPO-2 Electrolyte (10 mL)
Oxygen Sensors
FORT-100 Transducer
L-shaped sensors for tissue bath &
cell culture studies
The ISO-NOP30-L is a unique
L-shaped nitric oxide sensor
designed specifically for use in
tissue bath studies and similar
applications (e.g., see WPI’s
MYOBATH). The shape of the sensor
has been engineered to facilitate
placement of the electrode within
the lumen of the tissue vessel under
study. The ISO-NOP70-L is similar
in construction to the ISO-NOP30
but with the advantage of having
a flexible tip (70 µm diameter).
The ISO-NOPF200-L10 is designed
specifically for cell culture studies.
The ISO-HPO-100 is a 100 micron tip diameter hydrogen peroxide
micro sensor designed for use in tissues and similar applications. The
sensor design is based on a flexible “activated” carbon fiber sensing
electrode coated with a proprietary membrane that enhances hydrogen
peroxide detection.
Temperature Sensor
The temperature sensor (#ISO-TEMP-2) is based on a 2.0 mm tip
diameter high quality miniature platinum RTD (Resistance Temperature
Detector) electrode. This design has been shown to provide greater
accuracy, stability and interchangeability during temperature
measurements than traditional thermistor and thermocouple sensors.
The ISO-TEMP-2 is included with the purchase of a system.
ISO-TEMP-2 2 mm Platinum RTD Temperature Sensor (requires #91580)
91580
Microsensor Adapter Cable
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Multi-Port Measurement Chamber
Temperature stabilized four-port closed chamber
for measurements of nitric oxide, oxygen hydrogen
peroxide and other species in cell culture
● Four port (WPI #NOCHM-4) chamber accommodates WPI’s 2-mm sensors for
nitric oxide (ISO-NOP), oxygen (OXELP), hydrogen peroxide, and WPI’s KWIKTIP ion selective electrodes in combination with WPI’s 2 mm Dri-Ref™ reference
electrodes.
● Two additional top ports for injection of reagents using WPI’s MicroFil™
syringe needles
● Closed chamber design greatly reduces the surface area of the solution
exposed to air
● One top port and up to three side ports configuration provides adequate space
for convenient sample and electrode manipulation
● Temperature control through an external circulating bath
● The chamber can be used for nitric oxide and other species calibration at
temperatures from 4-40 ºC
Four-Port Closed Chamber for use with WPI’s 2.0 mm electrodes (e.g., ISO-NOP and OXELP, etc.)
Spare Plug-adapter for ISO-NOP nitric oxide electrode
Spare Center Chamber Gasket (package of 5)
S-nitrosoglutathione
Pre-polarizer ISO-NOP
Keep extra NO sen­sors
Rejuvenator
ready to use
GSNO has been identified in vivo as a potential
storage and transport vehicle for NO in the
body. GSNO has been used in clinical trials to
treat a form of preeclampsia and to prevent
platelet aggregation. It also has considerable
potential as NO donor in medicine.
M.W.336.3 • C10H16N4O7S • Purity > 98% •
Soluble in water or DMSO • Storage: -20°C
GSNO-50 GSNO 50 mg vial
GSNO-100 GSNO 100 mg vial
S-Nitroso-N-acetyl-Dpenicillamine
Achieve a stable background cur­rent quickly. This
small battery-powered de­vice applies a potential
to the NO electrode equivalent to the po­ten­tial ap­
plied by the ISO-NO meter. Con­se­quent­ly, a sensor
which has been con­nect­ed to the activator may be
transferred to the meter for im­me­di­ate­use. For use
with all WPI NO electrodes.
After an ISO-NOP 2‑mm sensor is used for long
periods, sensitivity may become reduced and
response time may increase. This little device can
restore ISO-NOP performance to original levels by
applying an electric waveform for a few seconds.
9v alkaline battery included.
NSA-3
JUV
ISO-NO Activator
ISO-NOP Rejuvenator
SNAP is a stable
green crystalline
S-nitrosothiol
compound that
mimics the ac­tion
of nitric oxide in vivo. It has vasodilatory
properties and has been shown to relax
isolated bovine coronary artery rings by
activating soluble gran­u­late cyclase. This
reagent also actuates apoptosis in mouse thy­
mocytes and has been accounted for reversible
inactivation of pro­tein Kinase C. SNAP can be
used for calibration of all WPI NO sensors.
M.W. 220.2 • Purity > 98% by NMR or TLC
SNAP25
SNAP50
SNAP100
SNAP, 25 mg vial
SNAP, 50 mg vial
SNAP, 100 mg vial
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
57
BIOS EN S I NG
NOCHM-4
NOCHM-P
800100-5
BIOS EN S I NG
Four-Channel Free Radical Analyzer
l Real-time detection using electrochemical
microsensors.
l Measure nitric oxide from < 0.3 nM to
100 µM.
l Integrated system includes one temperature
sensor, your choice of two additional
sensors, and a start-up kit.
l Measure hydrogen peroxide < 10 nM to
100 mM.
l Measure up to four different species in
the same preparation or simultaneous
measurement in four different preparations.
l Measure glucose
l Current measurement range from 300 fA to
10 µA (four ranges) permits wide dynamic
range for detection.
l Isolated architecture allows Lab-Trax
interface to simultaneously measure free
radical and independent analog data (i.e.,
ECG, BP, etc.) data on any channel.
l Wide bandwidth allows recording of fast
events.
Real-time detection and measurement of a
variety of redox-reactive species is fast and
easy using the electrochemical (amperometric)
detection principle employed in the new
TBR4100. This optically isolated four-channel
free radical analyzer has ultra low noise and
independently operated channels.
For use with WPI’s wide range of nitric oxide,
hydrogen peroxide, hydrogen sulfide and
oxygen sensors, the TBR4100 can measure
four different species simultaneously in the
same preparation. Simply plug a sensor into
any one of the input channels on the front
panel and select the current range. Poise
voltage can be selected from a range of values
tuned for optimal response from WPI sensors.
An independent output for real-time monitoring
of temperature is also included.
The TBR4100 analyzer utilizes PC-based
data acquisition via our Lab-Trax interface;
58
l Measure hydrogen sulfide
l Measure oxygen from 0.1% to 100%.
data traces are displayed and recorded in
real-time. The Data-Trax software comes
pre-configured for single or multiple electrode
recording; filters, gains, and smoothing are
all set for optimal results. Data can be viewed
making adjustments to smoothing and filter
settings without affecting the original stored
raw data. Electrode calibration from multiple
concentration readings can be input into the
software's Multipoint Calibration utility quickly
provides a plot and slope calculation for
electrode sensitivity determination. Alternately,
the Lab-Trax T series data interface
can be used for providing simultaneous
acquisition of Free Radical data along with
other physiological data (ECG, HR, BP, etc.)
as each of the four input channels has its
own independent input amplifier, filters, and
24-bit converter. (See WPI website for more
information on Lab-Trax data acquisition.)
Don’t need four channels? The single-channel
TBR1025 packs the power of its big brother in a
small, economical package.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
BIOS EN S I NG
Multipoint electrode calibration and slope determination can be quickly
derived from recorded calibration data.
TBR4100 SPECIFICATIONS
Power.......................................................100 ~ 240 VAC, 50-60 Hz, <15 W
Operating Temperature (ambient).........0 - 50°C (32 - 122°F)
Operating Humidity (ambient)...............15 – 70% RH non-condensing
Warm up Time........................................<5 minutes
Dimensions.............................................135 X 419 X 217 mm (5.25” X 16.5” X 8.16”)
Weight.....................................................1.35 kg (3 lb)
Display Functions...................................18 mm (0.7”) LCD readout, 4.5 digit
Polarization Voltage (mV)
Current input (nA, μA)
Controls...................................................Power (on/off)
Current Input Range
Polarization Voltage
Analog Output Range.............................+/- 10 V (continuous)
Analog Output Impedance.....................10 kohm
Channel to Channel Isolation.................>10 Gohm
Channel to Output Isolation...................>10 Gohm
Power Supply to AC Line Isolation.........>100 Mohm
Analog Output Drift................................<10 pA/h
Temperature Input
Number of Channels......................................1
Sensing Element.............................................Platinum RTD, 1000 Ohm
Range ..........................................................0-100°C
Accuracy..........................................................+/- 1°C
Resolution.......................................................0.1°C
TBR4100-416
TBR4100-424T
TBR1025
Analog Output................................................31.25 mV/°C (continuous)
Amperometric Input
Number of Amperometric Channels...............................4
Signal Bandwidth.............................................................0-3 Hz
Polarization Voltage (selectable via rotary switch)
Nitric Oxide........................................................865 mV
Hydrogen Sulfide...............................................150 mV
Hydrogen Peroxide............................................450 mV
Glucose...............................................................600 mV
Oxygen...............................................................700 mV
ADJ (user adjustable).........................................+/- 2500 mV
Polarization Voltage Accuracy.........................................+/- 5 mV
Polarization Voltage Display Resolution........................+/- 1mV
Current measurement Performance
Range
Analog Output
Noise @ 3Hz * Noise @ 0.3 Hz *
+/- 10 nA
1 mV / 1 pA
< 1 pA
< 0.3 pA
+/- 100 nA
1 mV / 10pA
< 7 pA
< 3 pA
+/- 1 μA
1 mV / 100pA
< 70 pA
< 30 pA
+/- 10 μA
1 mV / 1μA
< 700 pA
< 300 pA
*Instrument performance is measured as the (max-min) over 20 seconds
period with open input. Typical values are given at 3 Hz and 0.3 Hz
bandwidth.
Typical sensor performance with TBR4100
ISO-NOPF100 noise........................................................0.2 nM NO (<2 pA)**
**Sensor noise is measured as the (max-min) over a 20 seconds period with
the sensor immersed in 0.1 M CuCl2 solution.
Four-Channel Free Radical Analyzer with Lab-Trax 4/16 Data Acquisition System
Includes TBR4100 analyzer & power cord, Lab-Trax-4/16 data acquisition system & USB cable, 4 BNC cables,
3 electrode adapter cables, 1 temperature probe, 2 sensors of your choice, and sensor start-up kit(s), if applicable.
Four-Channel Free Radical Analyzer with Lab-Trax 4/24T Data Acquisition System
Includes TBR4100 analyzer & power cord, Lab-Trax-4/24T data acquisition system & USB cable, 4 BNC cables,
3 electrode adapter cables, 1 temperature probe, 2 sensors of your choice, and sensor start-up kit(s), if applicable
Single-Channel Free Radical Analyzer — Includes 1 sensor of your choice & 1 start-up kit
RECOMMENDED ACCESSORIES
SNAP50
SNAP S-Nitroso-N-acetyl-D-penicillamine, 50 mg vial
See ProGuide Electrode Positioners, page 73.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
59
™
Records redox
currents with
carbon fiber
micro­elec­trodes
Advanced
Neurotransmitter
Detection
BIOS EN S I NG
Measures oxidizable compounds
such as catechola­mines (epi­
neph­rine, norepinephrine,
dopamine), indolamines
(serotonin, melatonin), ascorbic
acid and Fe (II) with ex­quis­ite
sensitivity, low noise and site
specificity
Use with WPI electrodes — see next page
MicroC, WPI’s low cost and elegant in­stru­ment for elec­tro­chem­i­cal
detection using carbon mi­cro­elec­trodes, will record the pres­ence and
con­cen­tra­tion of oxidizable bi­o­log­i­cal compounds in vivo and in vitro.
It also features in­her­ent­ly low noise and a sen­si­tiv­i­ty of 1 mil­li­volt per
picoampere of oxi­da­tion cur­rent. Re­sponse time to quan­tal cat­e­chola­mine
re­lease is less than 1 mil­li­sec­ond. When used with car­bon fiber mi­cro­elec­
trodes, redox cur­rent can be re­cord­ed over a range of 1‑picoampere to 2
MICROC SPECIFICATIONS
Method
2 electrode, DC potentiostat
APPLIED POTENTIAL
0.65 V, variable ± 2.5 V
Current ranges
2000 pA, 20 nA, 200 nA, 2 µA
BANDWIDTH
1.67 Hz, 167-1000 Hz
NOISE
< 1 pA
Display31⁄2-digit LCD display, ±2 V
Recorder Output
± 4.5 volts
Rise Time
< 1 millisecond
Electrode probe/cable length Triax shielded, 5 feet
Power
Six 1.5 volt alkaline batteries (included)
Battery Life
> 1000 hours, est.
Shipping weight
4 lb (1.8 kg)
60
mi­cro­am­peres. The built-in carbon electrode
activation feature allows the easy re­new­al of
electrode sensitivity. In ad­di­tion, MicroC fea­tures
a low-pass fil­ter and the op­tion of ap­ply­ing DC
po­ten­tial ex­ter­nal­ly. A wide range of com­pounds
can be de­tect­ed: dopam­ine, epi­neph­rine, nore­
pi­neph­rine, se­ro­to­nin, etc. Other com­pounds,
such as glutamate, glu­cose, ace­tyl­cho­line and
alcohol, can also be detect­ed with MicroC using
en­zyme-modified biosensors.
See Application Notes, “Carbon Fiber
Microelectrodes”, available as a PDF file which
may be downloaded from WPI”s web site.
The MicroC Potentiostat is supplied with a
carbon electrode probe, with 5 feet triax cable, which accepts 0.79 mm
connector pin, and a ref­er­ence elec­trode with a 4 mm Ag/AgCl half cell
(see page 121). For applica­tions where small­er half cells are needed,
please call WPI for more in­formation.
References
G. A. Gerhardt, “Nafion-coated electrodes with high selectivity for CNS elec­tro­chem­
is­try” Brain Re­search, 290: 390-395 (1984).
R. M. Wightman, et al., “Temporally resolved cat­e­chola­mine spikes cor­re­spond to
sin­gle ves­i­cle release from individual chromaffin cells.” Pro. Nat’l Acad. of Sci. 88:
10754-58, (1991).
Z. Zhou and S. Misler, “Action Potential-in­duced Quan­tal Secretion of Cat­e­chola­
mines from Rat Adrenal Chromaffin Cells”, J. Biol. Chem. 270; 3498-3505, (1995).
SYS-MICROC Potentiostat
MICROCP
Replacement Probe for MicroC
OPTIONAL ACCESSORIES
300305
ATP Adapter (0.031" pin to 1 mm socket)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]com
US: Tel: 941-371-1003 • [email protected]
Carbon Fiber Mi­cro­elec­trodes
● Sensitive, renewable/durable and economical
carbon fiber electrodes for electrochem­i­cal
de­tection of oxidizable compounds
Potential (V)
50
Carbon fiber microelectrodes have been used in both the detection of
ox­i­diz­able compounds (Gonon, et al., 1978; Cahill and Wightman, 1995)
and extracellular single-unit re­cord­ing (Armstrong-James and Millar,
1979). WPI’s ultra-sensitive and low-noise carbon fiber (CF) electrodes can
be ap­plied, with our Micro-C Poten­tio­stat or similar in­stru­ments, in the
electrochemical detection of cat­e­chola­mines (epi­neph­rine, norepinephrine
and dopamine), indolamines (serotonin, 5-HT or melatonin), ascorbic acid,
Fe (II), and other ox­i­diz­able compounds.
Dopamine Concentration/Response Curve
1000000
Current Output (pA)
100000
10000
1000
100
10
1
0.1
1
10
100
1000
10000
100000
1000000
Concentration (ng/ml)
Fig. 1 — Excellent linearity in the response of carbon fiber electrode (CF30-500) to dopamine recorded on Micro-C. Courtesy: Drs.
D. Yeomans and X.-T. Wang, Uni­ver­si­ty of Illinois at Chicago.
-50
-100
0
5
10
Time (ms)
Fig. 2 — Extracellular recording using a carbon electrode in CA1
region of the hippocampus in an anesthetized rat shows ultralow noise (‑<5 µV‑). Cour­te­sy: Dr. Carolyn Harley of Memorial University,
Newfoundland, Canada.
References
P. S. Cahill, R. M. Whightman, Anal. Chem., 67, 2599-2605 (1995).
F. Gonon, et al., Hebd Seances Acad. Sci. Ser. 286, 1203 (1978).
M. Armstrong-James, J. Millar, J. Neurosci. Methods, 1, 279 (1979).
CARBON FIBER MICROELECTRODES, UNCOATED
Diameter Length CF10-100
10 µm
100 µm
CF10-250
10 µm
250 µm
CF10-500 *
10 µm
500 µm
CF30-50 *
30 µm
50 µm
CF30-100 30 µm
100 µm
CF30-500 *
30 µm
500 µm
CF30-1000 *
30 µm
1000 µm
(pack of 5)
CARBON FIBER MICROELECTRODES, NAFION-COATED
Diameter Length (pack of 5)
CFN10-50 *
10 µm
50 µm
CFN10-100 *
10 µm
100 µm
CFN10-250 *
10 µm
250 µm
CFN30-50 *
30 µm
50 µm
CFN30-100 *
30 µm
100 µm
CFN30-250 *
30 µm
250 µm
CFN30-500 *
30 µm
500 µm
CFN30-1000 * 30 µm
1000 µm
* Built to order — allow up to 4 weeks manufacturing time.
glass insulation
1 mm O.D. glass pipette or 2 mm plastic tube
Gold-plated connector pin
0.031 in. (0.79 mm)
30 µ or 10 µ
carbon fiber
Carbon fiber microelectrodes, ap­prox­i­mate­ly 25-50
mm (1-2 in. ) long, feature a gold-plated pin for con­
nec­tion to the probe.
cement
25-1000 µm
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
61
BIOS EN S I NG
CF electrodes (diameter of 10 or 30 µm) respond with an ex­cel­
lent linear­i­ty to the oxidizable compounds (Fig. 1) and can detect
the compounds as low as 0.2 nM. While the shorter (25-100 µm) CF
elec­trodes are suitable for in vivo amperometric and voltammetric
measurements, the long­er CF electrodes provide higher sen­si­tiv­i­ty and
are especially useful for the in vitro studies (amperometric or differential
pulse voltammetry). When used with the Micro-C Potentiostat, these CF
electrodes can be ac­ti­vat­ed and re­newed in sensitivity for multiple use.
The se­lec­tive detection of cat­e­chola­mines can be achieved with our
Nafion-coated CF electrodes. For se­lec­tive detection of 5-HT and ascorbic
acid, please contact WPI for more in­for­ma­tion.
0
O2 Sensor
for Real-time
Analysis
Fluorescence-based optical
sensor system
BIOS EN S I NG
The new oxygen sensing system measures fluorescence lifetime, phase
and intensity, using LED excitation and photodiode detection with filterbased wavelength selection. The system is simple to set-up and control.
The compact, self-contained unit makes it invariant to fiber bending and
stray light. It also has a wide dynamic range of optical intensity, as well as
low optical and electronic crosstalk, and low drift and phase noise.
When stability and sensitivity to drift are important in your oxygen sensing
experiment, this unit is ideal. It is perfect for applications where sample
set-ups must be left undisturbed for long periods of time.
Three coatings available for probes and patches
The optical sensors consist of transducer materials, applied to the tips of
optical fibers or to substrates such as patches or cuvettes, which change
optical properties in response to specific analytes in their immediate
environment.
OXY–The standard oxygen sensor designed for monitoring oxygen
partial pressure in gas and aqueous solutions is a fiber optic fluorescence
probe with a proprietary oxygen sensing coated tip.
HIOXY–Designed for monitoring oxygen partial pressure in nonaqueous vapors and solutions. The sensor coating chemistry is compatible
with oils, alcohols, and hydrocarbon-based vapors and liquids.
FLOX is a device for measuring fluorescence lifetime, phase and intensity.
It uses LED excitation and photodiode detection with filter-based
wavelength selection for easy experimental set-up and control. Because
the unit is self-contained, it is invariant to fiber bending and stray light,
and has a wide dynamic range of optical intensity as well as low optical
and electronic crosstalk, and low drift and phase noise. FLOX is especially
useful for oxygen sensing applications where stability and sensitivity to
drift is important and where sample set-ups must be left undisturbed for
long periods of time.
FOSPOR–A new generation of highly sensitive sensor coating can be
used for monitoring traces of oxygen in gas and liquids.
The oxygen sensor probes are low-power and offer high sensitivity,
reversibility and stability, ideal for remote monitoring. The thin coating
on the probe tips consumes no oxygen, allowing for continuous contact
with the sample. They are ideal for viscous samples and are immune
to interference caused by pH, ionic strength or salinity fluctuations or
biofouling.
l Fluorescence-sensing detector for optical sensors, a viable
alternative to traditional chemical sensing devices
l Self-contained, benchtop system–invariant to fiber bending or
stray light
l Affordable–Half the cost of comparable phase measurement
systems
l Excellent stability, extremely low drift and phase noise
l Simple calibration, setup and control
INTRODUCTORY PRICE
FLOX-PATCH Non-Invasive Oxygen Monitoring Kit, including phase measurement
system, temperature probe, 4mm HIOXY sensor patches
FLOX-PROBE In Situ Oxygen Monitoring Kit, including phase measurement system, temperature probe, and HIOXY O2 sensor
62
FLOX software displays oxygen measurements on a connected PC.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
AL300
A 500 µm OD (300 µm core diameter)
aluminum-jacketed optical fiber probe
for applications that require fine spatial
resolution. 300 µm alµminµm-jacketed
fiber assembly; 500 µm OD, 1 m length
OR125G and OR125GT
These probes are 1/8" OD optical
fiber probes used as direct replacement for 1/8" OD O2 electrodes.
1000 µm optical fiber, stainless steel
ferrule; 3.175 mm OD, 63.5 mm
length
OR125
The OR125 is a 1/8" OD optical fiber
probe used as a direct replacement for
1/8" OD O2 electrodes.
100 µm optical fiber, stainless steel
ferrule; 3.175 mm OD, 63.5 mm length
R Sensor Probe
1000 µm fiber in a stainless steel
1/16" ferrule; 1.587 mm OD,
152.4 mm length
P1600
BIOS EN S I NG
The PI600 is a silicone-jacketed,
polyimide-coated optical fiber probe
used in environments where a nonmetallic probe is required.
200 or 600 µm optical fiber with
silicone jacketing; 710 µm OD, 2 m
length
FLOX Specifications
Probe Specifications
OXY Formulation
(general-purpose coating)
FOSPOR Formulation
(high-sensitivity for low levels of O2)
HIOXY Formulation
(for hydrocarbon environments)
O2% range (at 1 ATM)
DO range (ppm at 1 ATM)
Temperature range
0-60 ºC for patches
O2% resolution
DO resolution (at room temp)
O2% accuracy
DO accuracy
Min. detectable level in gas
Response time (in gas)
0-100%
0-40 ppm
-50-+80ºC for probes
0-60ºC for patches
100 ppm in gas
4 ppb
<5% of reading
<5% of reading
0.01% - 0.05%
< 1 second
0-<10%
0-4 ppm
0-+60ºC for probes
NA
10 ppm in gas
0.4 ppb
<5% of reading
<5% of reading
0.001% - 0.01%
< 30-60 second
0-20.9%
0-8 ppm
-50-+60ºC
(with overcoating in gas)
45-60 seconds
60-90 seconds
(in pure water)
30-45 seconds
60-90 seconds
100 ppm
4 ppb
<5% of reading
<5% of reading
0.01% - 0.05%
< 1 second
~45 seconds
Patch Specifications
OXY Formulation
FOSPOR Formulation
HIOXY Formulation
02% range (at 1 ATM)
DO range (ppm at 1 ATM)
Temperature range
02% resolution (20 sec averaging)
(at 30 sec averaging)
DO resolution (at room temp)
02% accuracy
DO accuracy
Min. detectable level
(at 30 sec averaging)
Minimum detectable level in water
0-100%
0-40 ppm
-20 to +60 ºC for patches
0.05%
0.05%
20 ppb
5% of reading
5% of reading
0.1% O2
0.1% O2
40 ppb
0-10%
0-4 ppm
0 to +60 ºC for patches
0.01%
0-20%
0-8 ppm
-20 to +60 ºC for patches
4 ppb
5% of reading
5% of reading
0.01% O2
20 ppb
5% of reading
5% of reading
4 ppb
40 ppb
(room temperature)
Response time (in gas)
(with overcoating in gas)
(in pure water)
<1 second
~30-45 seconds
~45 seconds
30-60 seconds
~60-90 seconds
~60-90seconds
<1 second
~30-45 seconds
Stability (Continuous LED)
OXY Formulation
FOSPOR Formulation
HIOXY Formulation
Lifetime Stability (Tau)
Oxygen Stability %
0.0006 usec/ HR
0.01% HR
0.003 usec/ HR
0.005% HR
0.0002 usec/HR
0.007% HR
Sterilization Guide
OXY Formulation
FOSPOR Formulation
HIOXY Formulation
Form factor
Autoclavable
Calibration
Gamma
Probes/Patches
No
Probes/Patches
Not yet determined
Yes
Not yet determined
Probes
Yes
Single point reset required after sterilization
Yes
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
63
OxyMini & OxyMicro
A new generation of fiber optic oxygen
sensors based on luminescence lifetime
The OxyMini system is optimized for process control and biotechnology applications.
The OxyMicro is designed for biological research applications including implanting
into tissues, cell cultures, profiling of biofilms and sediment related bioassays. The
measurement principle of the sensor system is based on the detection of oxygen
concentration as a function of luminescence lifetime either in dissolved or gaseous
phase environments.
BIOS EN S I NG
OxyMini and OxyMicro Benefits
MicroFlow
The MicroFlow fiber optic oxygen sensor (WPI
#501657) is a miniaturized fiber optic chemical
sensor optimized for fast response time (t90
< 1 sec in gases, < 5 sec in liquids). The tiny probe has a tip size of 50 µm and
is integrated in a T-shape flow cell for easy connection via Luer-Lock adapters to
external tubings. Liquids (like water, blood, etc.) can be pumped through the cell.
MicroImplant
The MicroImplant fiber optic oxygen sensor (WPI #501658) is an implantable probe
(IMP) with a tiny probe tip size 50 µm, an exposed fiber length of 5‑mm and a jacket
diameter of 900 µm. The IMP sensor was successfully implanted in crabs, fishes
and soil.
OxyMini systems
The OxyMini is a single-channel fiber optic oxygen meter for WPI’s fiber optic oxygen
minisensors. These sensors are based on 2 mm polymer optical fibers and have a
length of 2.5 m. A wide range of applications is possible with these sensors.
● Process control: bottling plant in breweries and quality control of packages
● Biotechnology: Control of cell culture media and non-invasive control of bioreactors
● Implantation of oxygen sensors into soil and trees.
●Oxygen is not consumed during the experiment
●Immune to electrical and magnetic interference
●Excellent long-term stability
●No lengthy polarization necessary (e.g., as Clark-type oxygen electrodes require)
●Fast response time < 0.5 s for MicroTip sensors
●Probe size of MicroTip sensors as small as 50 µm
●Measurement is feasible in dry gas
●Optical isolation of sensor tip available for fluorescent or photosynthetically active samples
This oxygen dipping probe (WPI #501641) has a
tip diameter of 4 mm and consists of a polymer
optical fiber, with an oxygen sensitive coating.
The MiniTip’s range is 0 to 100%. This robust sensor has a response time (t90) of
approximately 40 s.
Measurement Principle
MiniFlow
Conventional fiber-optic oxygen sensor systems based on intensity measurements
are limited in their accuracy by light source stability and ambient light fluctuations.
Using a luminescence lifetime detection, measurements are not affected by light
source stability, intensity fluctuations caused by fiber bending or changes of the
optical properties of the sample (turbidity, refractive index, coloration, etc.). These
advantages make WPI’s OxyMini and OxyMicro the most advanced and reliable fiberoptic oxygen system available.
The MiniFlow oxygen probe (WPI #501642) is a
miniaturized fiber optic chemical sensor integrated in
a T-shape flow through cell. The standard T-shape flow
cell can be easily connected via Luer-Lock adapters to external tubings. Liquids (e.g.,
water, blood, etc.) can be pumped through the cell. The sensor has a response time
(t90) of approximately 40‑s and an excellent long-term stability.
Calibration: The sensors can be calibrated by a simple two point calibration, 100%
air-saturation and 0% air saturation.
OxyMini and OxyMicro oxygen meters: The OxiMini and OxyMicro fiber optic
oxygen meters are compact, easy to transport. The instruments are designed for
in/outdoor use and can be connected to a PC via a RS232 interface. Data can be
visualized, analyzed and stored with the supplied software. A full range of sensors
covering most biomedical applications are available.
MiniTip
MiniFoil
WPI offers the sensor material on a 1 cm2 support disk made of
polyester. This material can be glued, for example, inside glass vials
and the oxygen concentration can be measured non-invasively and
non-destructively from outside through the wall of the flask. A plastic
fiber optic cable (WPI #501644, WPI #501645) is used to illuminate
the sensor foil. The wall of the flask must be transparent/non-fluorescent. Response
time (t90) of approximately 50 s. The material can be implanted into animal tissues
or custom-made housings.
MINISENSOR SYSTEM
OXY-MINI-AOT Fiber-optic Oxygen Meter for Minisensors *
Minisensors (not interchangeable with Microsensors)
501641
MiniTip, fiber-optic oxygen sensor 501642
MiniFlow, flow-through cell with integrated planar oxygen sensor
503090
MiniSpot, planar oxygen-sensitive spot, 5 mm diam. (includes 10)
Requires #501644
501644
Polymer optical fiber with 1 SMA connector
OxyMicro systems
The OxyMicro is a single channel fiber
optic oxygen meter for WPI’s fiber optic
oxygen microsensors. Applications include:
● Oxygen profiles of marine sediment, soils, or tissue
● Implantation into living tissue (e.g., heart or muscle tissue)
● Control of cell culture media in Biotechnology.
MICROSENSOR SYSTEM
OXY-MICRO-AOT Fiber-optic Oxygen Meter for Microsensors *
MicroTip
The MicroTip (WPI #501656) is a needle-type (27 ga.) oxygen micro sensor designed
for applications where a small tip size of 50 µm and a fast response time (t90) of 1
s are necessary. The oxygen sensitive sensor tip consists of 140 µm fiber tapered
to a 50 µm tip. The sensor is housed inside a
stainless steel needle of 22 mm length and 0.4
mm diameter. This allows penetration through a
septum rubber or similar material. These sensors
are ideal for oxygen profiling in sediments and
biofilms.
Measurement Range
dissolved/gaseous
Response Time [t90]
dissolved/gaseous
Sterilization (EtOH, H2O2)
autoclavable (130°C, 1.5 atm)
Drift (100,000 datapoints, 20°C)
Accuracy (20°C)
Resolution (20°C)
Temperature Range
Microsensors (not interchangeable with Minisensors)
501656
MicroTip, needle-type housing fiber-optic oxygen sensor, 50 µm tip
501656-C MicroTip, needle-type housing, 50 µm tip, optical isolation
501656-F MicroTip, needle-type housing, 140 µm flat tip
501657
MicroFlow, flow-through housed oxygen microsensor
501658 MicroImplant, implantable oxygen microsensor, 50 µm tip
501658-C MicroImplant, 50 µm tip, optical isolation
501658-F MicroImplant, 140 µm flat tip
*Meter contains two analog outputs and one trigger input
MiniTip
MiniFlow
MiniSpot
MicroTip
MicroFlow
MicroImplant
0-45 ppm, 0-100%
0-760 mmHg
0-45 ppm, 0-100%
0-760 mmHg
0-45 ppm, 0-100%
0-760 mmHg
0-45 ppm, 0-100%
0-760 mmHg
0-45 ppm, 0-100%
0-760 mmHg
0-45 ppm, 0-100%
0-760 mmHg
40 s
10 s
40 s
10 s
40 s
10 s
<2s
< 0.5 s
<2s
< 0.5 s
<2s
< 0.5 s
Y
N
Y
Y
Y
Y
Y
N
Y
Y
Y
Y
< 0.1%
< 0.1%
< 0.1%
< 0.3%
< 0.3%
< 0.3%
0.2%
2.75 ±0.01 ppm, 9.00 ±0.05 ppm, 220 ±0.15 ppm, 45.0 ±0.25 mmHg, 150 ±0.75 mmHg, 375 ±2.6 mmHg
-10°C to 50°C
2.5 m
World Precision Instruments
www.wpiinc.com
Probe Assembly Length
64
UK: Tel: +44 (0)1462 424700 • [email protected]
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
pHOptica™
A novel fiber optic pH
system
pHOptica™ is a pH measuring system which
uses fiber optic sensors and patented DLR
technology. This method allows referenced
measurements with single excitation to be
implemented.
Features of pHOptica Meter
Features of
pH optical sensors
● No reference electrode is needed
● Immune to electrical interferences and magnetic fields
● Low drift
● High spatial resolution due to small tip size
● Measurement in very small sample volumes
● Additional optical isolation of the sensor tip is available for
measurements in colored or photosynthetically active samples.
pHOptica Micro system
pHOptica Mini system
The pH Optica micro system is a single channel pH system for use with fiber optic
micro sensors. The applications include:
The pH Optica mini system is a single channel
pH system for use with fiber optic mini
sensors, foil and spot surface sensors. The
applications include:
● Penetration or implantation into living tissue (heart, muscle or animal blood
vessels).
● Non-invasive and non-distractive pH
measurements from outside through flask
walls (cell culture)
● Soil implantation for pH measurement.
● Implantation into customer-made housing.
To protect the small
glass fiber tip against
breaking, suitable
housings and tubings
around it, depending
on the respective
application, were
designed.
● Online pH monitoring by flow through cells
● Dipping probe pH measurements
pH mini sensors
● OD of the dipping sensor is 4 mm. ● Sterilization of the pH sensor spots via
gamma radiation. ● The pH mini sensor meter is based on 2 mm PMMA waveguides.
● Drift of 0.1 pH units for 10,000 measurements (4 days measurement in the 30 sec
data update mode).
Two different housings and sensor spots (sensorfoils) are offered:
Needle-Type Housing Sensor—
the glass-fiber with its pH-sensitive
tip is protected inside a stainless
steel needle (18 ga.); fiber has to
be extended during measurement;
penetration through septum.
Implantable sensor—
without any housings
implantation into animal
blood circuits; soil implantation;
implantation in customer-made
housings
POF Coated with a pHSensitive Foil—Small
and robust pH dipping
sensor; no reference
electrode needed.
pH micro sensors
Flow-Through Cell
with Integrated
pH Sensor—On-line
monitoring; can be
easily connected via
Luer-Lock adapters.
Planar pH Sensitive Foils
and spots—non-invasive
and non-destructive
measurement from outside
through the wall of the
flask; online monitoring.
● Tip size 140 micrometer.
● Drift of 0.1 pH units for 2000 measurements (16 hours
measurement in the 30 sec data update mode).
pHOptica Specifications
Data Interface RS232
Sample Rate 1 sample per second
Measuring pH Range 5-9
Resolution (at 20 °C) ± 0.03 (microsensors);
± 0.01 (minisensors)
Response Time < 1 min
Dimensions 185 x 110 x 45 mm
Weight 630 g
Power Supply 100 - 220 V AC
MINISENSOR SYSTEM (cannot be used with microsensors)
PH-OPTICA-MINI Fiber Optic pH Meter for minisensors, foils and spots
503538* pH MiniTip, fiber optic pH sensor dipping probe, disposable (4 mm OD), pkg of 3 502120* pH MiniFlow, fiber optic pH flow sensor, pkg of 3 *Requires #503110
502122**pH MiniSpot, fiber optic pH spot sensors, pkg of 10, OD 5 mm **Requires #501644
501644 Polymer Optical Fiber with 1 SMA connector
503110 Fiber Optic Cable with 1 SMA connector
MICROSENSOR SYSTEM (cannot be used with minisensors)
PH-OPTICA-MICRO Fiber optic pH meter for microsensors
502123 pH MicroTip (needle-type), fiber optic pH sensors (140 µm OD), pkg of 3 502124 pH MicroImplant, fiber optic pH implantable sensor (140 µm OD), pkg of 3 World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
65
BIOS EN S I NG
● Single-channel, compact, easy to transport fiber-optic meter for pH
measurements with miniature sensors.
● Two 12-bit, programmable analog outputs, with electrical isolation.
● One external trigger input, with electrical isolation.
● Computer with RS232 interface required for operation.
● User-friendly software saves and visualizes measured values.
● Several pHOptica meters can be connected to one computer.
● Temperature variation is recorded using a temperature sensor.
Dissolved oxygen meter & oxygen electrode
Electrically isolated, the electrode can function in recording situations
where ground-referred electrodes would fail
● Measures gas and dissolved oxygen
● Low O2 consumption
● 2 mm tip
BIOS EN S I NG
Isolation prevents adverse interaction with other
electrodes and in­stru­ments. Highly accurate and stable
ISO2 mea­sures oxygen concentrations in aque­ous
solutions and in gas mixtures. Mea­sure­ment modes are
percent ox­y­gen, parts per mil­lion, and oxygen reduction
cur­rent in nanoamperes.
The lower detection limit (DL) for ISO2 (in gas­eous or
in liquid phase) is 0.1 ppm or 0.1%. Ox­y­gen con­cen­tra­
tion around 0.5 ppm or 0.5% and up can be routinely
mea­sured in the gaseous or in the liquid phases.
DL for a particular sensor tends to be different in
gaseous and liquid phases when the baseline noise
level is also dif­fer­ent. In the case of ISO2, however, the
baseline noise level is very close whether the elec­trode
is im­mersed in solution or used in the gaseous phase,
so DL for the ISO2 re­mains rel­a­tive­ly close in either
phase.
The small tip size (2 mm diameter) and low ox­y­gen
con­sump­tion of the OXELP electrode make it ide­al
for mea­sure­ments in vivo or in vitro. With a T-Adapter
(#5399), the sen­sor probe can be used also for con­tin­
u­ous-flow mon­i­tor­ing of ox­y­gen in small fluid volumes.
OXELP also fea­tures a fast response time, typically
10 sec­onds. Op­tion­al BNC-to-dou­ble banana adap­tor
(#13347) and BNC cable (#500184) allow ISO2 to be
con­nect­ed di­rect­ly to your chart record­er.
SYS-ISO2Dissolved Oxygen Meter & Electrode
OXELP Replacement Oxygen Electrode for ISO2
5378
Replacement Electrode Sleeve Kit (pkg of 4)
Four sleeves with membranes, plus 10 mL refill solution.
ISO2 Filling Solution (10 mL)
7326
5377
Replacement ISO2 Start-up Kit
Includes Calibration Bottle, 10mL Refill Solution, 1 cc Syringe, 2
Replacement Membranes Sleeves, MicroFil (28 ga.)
T-Adapter Flow-Through Kit
5399
Includes 3 female luer T’s, 3 luer lock fittings, 3 2mm gaskets, 6
male luer to 1/8-in. tubing, 3 luer lock fittings
Chart Recorder Adapter (requires BNC cable) 13347
500184 BNC Cable
ISO2 SPECIFICATIONS
MEASUREMENT MODES
% O2:
0-100%
ppm:
0-20
Current:
0-200 nA
RESOLUTION
0.1 ppm
ACCURACY± 1.5%
RECORDER OUTPUT
1000 Ω resistance for chart recorders
DISPLAY
3.5-digit LCD
POWER
2 nine-volt alkaline batteries, supplied
BATTERY LIFE
1000 hours, estimated
DIMENSIONS
8 x 4 x 2 inches (20 x 10 x 5 cm)
SHIPPING WEIGHT
5 lb (2.3 kg)
OXELP OXYGEN ELECTRODE
TIP DIAMETER
STAINLESS STEEL SLEEVE
OVERALL LENGTH
CABLE LENGTH
RESPONSE TIME
2 mm
66 mm
125 mm
122 cm (4 feet), including BNC connector
10 seconds, 90% response typical
in well-stirred solution
Also see WPI’s
temperature stabilzed
chamber, page 57.
66
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Beetrode
BEE-CAL
®
ZBEECAL
Micro pH Elec­trodes
with 100-micron sensing tips!
3508
● NEW—IMPROVED—super-miniature, superfast
coated wire pH electrodes
● 100-micron diameter — ideal for monitoring fast
pH changes in very small places
BEETRODE SPECIFICATIONS
NMPH3
NMPH2B
NM
P H2
3L
1
NM
H5
PH
PH
TIP DIAMETER
100 µ (0.1 mm)
TIP LENGTH
2 mm (approx.), except
5 mm (approx.) on NMPH5
BODY DIAMETER (NMPH 1, 3, 3L, 5)
0.187 in. (5 mm)
BODY LENGTH (NMPH 1, 3, 5)
1.875 in. (48 mm)
BODY LENGTH (NMPH 3L)
0.75 in. (19 mm)
RESPONSE TIME
1 s (90%) typical
pH RANGE
0-14
SLOPENernstian
RESISTANCE
100 kΩ (max)
No significant interference by K+, SELECTIVITY
Na+, Ca++ in 0.1 to 1 M solutions
Beetrode® is a solid state pH sensor with ideal characteristics over a
wide pH range. Exhib­its a larger Eo than conventional glass electrodes.
Requires a separate ref­er­ence electrode, such as WPI’s Dri-Ref Series.
Beetrodes (except the NMPH2) connect to your pH meter via a BNCterminated cable. Bee­trodes generate mV readings on standard pH meters.
To obtain pH-scale readings on stan­dard pH meters, use BEE-CAL™, a
small, battery-operated com­pen­sa­tor (AA battery included) that adjusts the
electrode offset po­ten­tial so that Beetrodes will produce standard pH-scale
readings. (NMPH2B requires ZBEECAL.)
pH-Silver
Low-noise combination pH electrode
WPI’s pH-Silver, single/double-junction, gel-sealed Ag/AgCl
combination pH electrodes are very low noise. They are designed
for both laboratory and field use, especially for applications
involving strong magnetic fields. The unique features of these
electrodes provide unparalleled performance.
NMPH1*
Beetrode—2 mm receptacle
NMPH2
Beetrode with 1 m cable (unterminated)
NMPH2B
Beetrode w/ BNC cable, 1 m cable
NMPH3*
Dental Beetrode, 45° Bend, 2 mm receptacle
NMPH3L*
Dental Beetrode w/ 2 mm loop, 2 mm receptacle
NMPH5*
Beetrode, 2 mm receptacle
SYS-BEECAL Beetrode Offset & Cable (BNC, 2 mm pin)
ZBEECAL
Bee-Cal Level Shifting Device
3508
BNC-to-US Standard Adapter
1358
BNC-to-2 mm Pin Adapter
JE671P
pH Meter, pH Electrode, Temp. Probe 110v AC adapter
JE671PZ
pH Meter, pH Electrode, Temp. Probe 220v AC adapter
*Requires BEE-CAL or 1358 BNC-to-2 mm Pin Cable (4-ft)
for connec­tion to a pH meter.
l Special internal fill offers full range linear temperature
compensation
l Very low sensor glass membrane resistance
l Zero and Isopotential: ~pH 7
l Compatible with almost all pH meters
PH-SILVER
JE671P
JE671PZ
l Superior stability
Combination pH Electrode, Low Noise
pH Meter, pH Electrode, Temp. Probe 110v AC adapter
pH Meter, pH Electrode, Temp. Probe 220v AC adapter
pH-Silver Specifications
l Fast response
l Minimum sodium (alkaline) error
l pH 0 -14 full range
measurement
Bulb ShapeBall
Body Material / Color
Epoxy / Blue
Type of ReferenceAg/AgCl
Sealed/RefillableGel-Sealed
pH range0-14
Junction Material
Ceramic wick
Junction TypeSingle
Max. Temperature
80 °C
Dimensions
12 x 115 mm
Cable Length
1.0 m
ConnectorBNC
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
BIOS EN S I NG
NMP
NM
1358
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
67
Dri-Ref™ Reference Electrodes
Although the internal filling solution con­tains KCI, the low flu­id leakage
means Dri-Ref may be used in combination with ion se­lec­tive elec­trodes,
in­clud­ing those for K+ and Cl-, without sig­nif­i­cant con­tam­i­na­tion from the
reference elec­trode.
50
DRIRE
F-4
DRIRE
The Dri-Ref electrodes are chemically re­sis­tant to strong acids and alkalines.
Dri-Ref electrodes are not suitable for use in organic sol­vents. In ad­di­tion, the
long, thin FLEXREF may be easily ma­nip­u­lated to ac­com­mo­date a difficult
ex­per­i­men­tal setup.
F-5
DRIRE
F-2
With a diameter of 2‑mm, SUPER-Dri-Ref does not leak electrolyte at all.
Exhibiting the electrical stability of a classic flow­ing junction reference cell, this
electrode exhibits low re­sis­tance and a stable half-cell po­ten­tial es­sen­tial­ly in­
de­pen­dent of sample electrolyte concentration. SUPER-Dri-Ref is ideal for small
vol­ume and low salt concentration mea­sure­ments.
REF
FLEX
DR2
ef, S
R
i
-Dr
F-L
S UPER
DRIRE
BIOS EN S I NG
DRIRE
Micro-Reference Electrode
F-5S
DRIRE
SUPER-Dri-Ref
H
Only 450 µm in diameter and 1 inch long, WPI’s new DRIREF-450 reference
elec­trode can be used along with other sensors in space-restricted areas and
very small sam­ple vol­umes.
Luer-Tip Reference
F-
2S H
Dri-Ref™ reference electrodes were developed by WPI to have
extremely low electrolyte leak­age properties, hence the name
“Dri-Ref”. In addition to this key feature, these electrodes exhibit
stable and reproducible potential and low resistance. Stored in
KCl when not in use, they have a long life expectancy.
The male luer fitting at the front of the DRIREF-L allows it to be easily
connected to a female luer port (see WPI’s luer fittings kit, page 138) to form a
tight seal — a very convenient installation for a flow-through system.
FLEXREF
DRIREF-2
DRIREF-2SH
DRIREF-5
DRIREF-5SH
SDR2
DRIREF-450
DRIREF-L
Flexible Dri-Ref, 1.5 mm diam.
Dri-Ref, 2 mm diam.
Dri-Ref, 2 mm diam. (Short)
Dri-Ref, 4.7 mm diam.
Dri-Ref, 4.7 mm diam. (Short)
SUPER-Dri-Ref, 2 mm diam.
Micro-Dri-Ref, 450 µm diam.
Reference Electrode with Luer Tip
DRI-REF SPECIFICATIONS
DRIREF-450
DRIREF-5DRIREF-2FLEXREF SDR2
DRIREF-LDRIREF-5SH
DRIREF-2SH
Length
2.54 cm
9 cm
13 cm
13 cm
9 cm
7.5 cm
3.5 cm
2 cm
Diameter
450 µm
4.7 mm
2 mm
1.5 mm
2 mm
Standard Luer
4.7 mm
2 mm
EpoxyIsoplast™
Teflon™
PVC Polypropylene
EpoxyIsoplast™
Construction Coated Glass
Lead Length
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
30 in. (76 cm)
CONNECTOR
2 mm pin
2 mm pin
2 mm pin
2 mm pin
2 mm pin
2 mm pin
2 mm pin
2 mm pin
Resistance (typical)
< 5 K Ω~500 Ω
~2.7 KΩ
~2.7 KΩ
< 5 KΩ~500 Ω~500 Ω
~2.7 KΩ
Filling Solution
KClKClKClKClKClKClKClKCl
~7.4×10-7~7.4×10-7~5.7×10-8
ELECTROLYTE Leakage (mL/hr)—
~7.4×10-7~5.7×10-8~5.7×10-8—
Isoplast is a trade mark of Dow Chemical. Teflon is a trade mark of DuPont.
Calcium Calibration Solutions
CALBUF-1
CALBUF-2
For use with calcium
electrodes
For use with calcium fluorescent indicators
CALBUF-2 is especially suitable for calibrating flu­o­res­cent Ca++ in­di­ca­
tors. It provides eleven buffer stan­dards in the 10-4 to 10-8 M Ca++ range,
where­as other commonly used flu­o­res­cent Ca++ indicators have the
ap­par­ent Kd in the range of 100 to 300 nM. As with any ionic sensitive
indicator, the sensitivity range of these indicators is about 1.0 log unit
above and below the Kd. CALBUF-2 provides seven calibration points in
this sensitivity range. It has an osmolarity of 0.305, which is isotonic with
most mammalian cells.
A set of eight calcium buffers
covering the range of concentration
from 10-1 to 10-8 M Ca++. Each
buffer contains 20 mL of solution
and enough potassium chloride
to set the ionic strength to 0.1 M.
Limited shelf life; use within 30 days.
Concentration: 1x10-1, 1x10-2, 1x10-3, 1x10-4, 1x10-5, 1x10-6, 1x10-7,
1x10-8 M at 20°C. Limited shelf life; use within 30 days.
CALBUF-1 Kit of 8 Calcium Buffer Solutions
68
Concentration: 1x10-8, 4x10-8, 1x10-7, 2.5x10-7, 5x10-7, 7.5x10-7, 1x10-6,
4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C. Ionic strength: 0.150 M. 11
bottles, 20 mL each. Limited shelf life; use within 30 days.
CALBUF-2 Kit of 11 Calcium Buffer Solutions
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
68
Kwik-Tip
Each kit
includes three
electrode tips.
™
Ion selective electrodes
ast al!
f
,
e
­c
t
ura o­nom­i
c
c
A
ec
and
● Superior, stable PVC membrane
● Fast response
● 2mm diameter tips
● Interchangeable tip holder
COMPLETE KITS
KWIKCAL-2 Holder & 3 Calcium Electrodes
KWIKH-2 Holder & 3 Hydrogen Electrodes
KWIKPOT-2Holder & 3 Potassium Electrodes
KWIKTPP-2Holder & 3 TPP (Tetraphenylphosphonium) Electrodes
BIOS EN S I NG
These highly stable electrodes ac­cu­rate­ly measure
cal­ci­um, po­tas­si­um, hydrogen and TPP ion activity.
Tips consist of 2 mm di­am­e­ter plas­tic tubes sealed
at one end with an ion-sen­si­tive mem­brane. After filling with elec­tro­lyte
so­lu­tion, the user in­serts the tube into the hold­er and con­nects it to a pH
meter. Tips and hold­ers are in­ter­change­able, so one tip may be re­placed
with an­oth­er sensitive to a dif­fer­ent ion. Replac­ing a tip takes less than a
minute. Elec­trode tips nor­mal­ly last sev­er­al months, when stored proper­ly
in sa­line so­lu­tion. When re­place­ment is neces­sary, only the tip need be
re­placed.
Kwik-Tip electrodes are available separately and as kits. Each “KWIK”
Elec­trode Holder kit includes a reus­able hold­er and three re­mov­able tips.
In addition to a 4-foot BNC ca­ble and an electrolyte filling syringe; “TIP”
Elec­trode Kits con­tain three elec­trode tips for a specific ion. A separate
ref­er­ence electrode, such as WPI’s Dri-Ref™, is also required.
If your pH meter requires a US Standard connector, also order Part
#3508 (BNC-to-US pH Standard adapter).
MicroFil filling
syringe (included).
HOLDERS AND REPLACEMENT TIPS
KWIK-2
Electrode Holder with BNC cable
TIPCA
Calcium Electrode Tips (3)
TIPH
Hydrogen Electrode Tips (3)
TIPK
Potassium Electrode Tips (3)
TIPTPP
TPP+ (Tetraphenylphosphonium) Electrode Tips (3)
ACCESSORIES
3508
BNC-to-US pH Adapter
JE671P
pH Meter, pH Electrode, Temp. Probe 110v AC adapter
JE671PZ
pH Meter, pH Electrode, Temp. Probe 220v AC adapter
Also see Dri-Ref Reference Electrodes
TIP ELECTRODE SPECIFICATIONS
RecommendedMin.
Part No.
Electrode
Color
Filling
Slope /
Concentration Selectivity
Code
SolutionDecade
Range Coefficients (-log)
TIPCA
0.1 M CaCl2
28 mV
0.1 M - 10-6.75 M
Na+ 5.5, K+ 5.4, Mg++ 4.9
TIPH
Hydrogen
Orange
1 M Citric Acid,
0.01 M NaCl, pH 5.6
54 mV
pH 5.0 - 12
Na+ 10.4, K+ 9.8, Ca++ 11.1
TIPK
0.1 M KCl
54 mV
0.1 M - 10-4.5 M
Na+ 4.0, Ca++ 3.9, Mg++ 3.0
Calcium
Potassium
TIPTPPTPP+
Green
Yellow
Purple
10 mM
TPP+
54 mV
0.001 M -
10-4
M
K+ 6.0
Liquid Ion Exchangers
CATIONS
ION
CATALOG NO.
H+
IE 010
K +
IE 190
Ca++
IE 200
When used in
micropipettes to
record cellular ion
concentrations,
consider using
WPI’s Duo 773
electrometer
(channel A).
SELECTIVITY COEFFICIENTS*
Na+
12.71.97 5.5
Mg++
— 2.954.9
K+
—— 5.4
Ca++
— 2.7—
USEFUL pH RANGE
2-10
4-10
4-10
SLOPE 56 mV
58 mV
28 mV
LINEAR RANGE
pH 4-12
pK 0-3
pCa 1-7
APPROX. EQUIV.
—
Corning
ETH1001
477317
*Selectivity Coefficients are expressed here as -log Kij or pKij.
69
IE010
IE190
IE200
Hydrogen Ion Exchanger (0.1 mL)
Potassium Ion Exchanger (1.0 mL)
Calcium Neutral Ion Exchanger (0.1 mL)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
69
47530 (without base)
47540 (with base)
ProGuide
47520 (without base)
47510 (with base)
Novel electrode holder
BIOS EN S I NG
Designed to hold WPI biosensors and any
standard pH electrode, the unique ProGuide
allows the positioning of an electrode in
small sample volumes without the need for
a micromanipulator. The smooth mechanism
easily moves an electrode side-to-side or
up and down while keeping it at a constant
vertical angle.
The ProGuide Plus (shown at right) comes with
a micrometer positioner which allows fine
adjustment of 0.01mm in your samples.
47520
47510
47530
47540
ProGuide Positioner/Holder
ProGuide Positioner/Holder with Base ProGuide Plus Positioner/Holder with Micrometer
ProGuide Plus Positioner/Holder with Micrometer with Base
pH Meter
Hard-to-find, classic pH meter JE671P
allows the scientist to control all the
parameters manually. Especially useful for
non-standard pH and ISE electrodes.
l All solid state design
l Low drift and high stability
l Fast input response
l Manual and automatic temperature
compensation
l Auto polarity mV measurement
JE671P SPECIFICATIONS
RANGE
pH. . . . . . . . . . . . 00.00 - 14.00
mV . . . . . . . . . . . -1999 to +1999
Temp. . . . . . . . . . 0.0 to +100°C
RESOLUTION
pH. . . . . . . . . . . . 0.01
mV . . . . . . . . . . . 1 mV
Temp. . . . . . . . . . 0.1°C
ACCURACY
pH. . . . . . . . . . . . ± 0.1
mV . . . . . . . . . . . ± 0.1%
Temp. . . . . . . . . . ± 0.5°C
70
JE671P
JE671PZ
pH Meter, pH Electrode, Temperature Probe, 110V AC Adapter
pH Meter, pH Electrode, Temperature Probe, 220V AC Adapter
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Amplifier
AC/DC
Differential Headstage
EMG
EKG
Stimulation Isolated
Multichannel
Battery
Powered
Connectors
Page
2 mm pin
72
2 mm pin
73
2 mm pin
74
Intracellular Bioamplifiers
FD223A
DC
Electro 705
DC
Duo773
DC
t
t
t
t
t
t
t
t
Extracellular Bioamplifiers
WPI’s Low-Noise Amplifiers Outperform Cheap Imitations
DAM50
AC/DC
t
DAM80
AC
t
t
t
t
ISO80
AC
t
t
t
t
DAM800
AC/DC
t
t
t
76
t
RJ-11
78
t
Mini Banana
79
t
Mini Banana
80
RJ-11
84
t
8-pin DIN WPI
transducers
91
t
Ussing 2 mm
15
t
t
t
Transducer Amplifiers
DC
t
AMPLIFIERS, ELECTROMETERS
TBM4M
Epithelial Voltage/Current Clamp Bio Amplifier
EVC4000
DC
t
Specialty Measurement
121 Window Discriminator
81
260 Dual Microiontophoresis Current Generator
81
900A Intracellular Pressure Measurement
90
ABM Audible Baseline Monitor
81
LPF30 Low-Pass Filter
81
Omega-Tip-Z Electrode Volt-Ohmmeter
81
Pressure Manometer
187
Accessories
Ag/AgCl Half Cells
119
Cables & Connectors
110
Force Transducers
87
Metal Microelectrodes
88
Transducers for Physiological Measurement — LUME, PNEU
86
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
71
AMPLIFIERS, ELECTROMETERS
Dual Channel Differential Electrometer
l High
input impedance (10 15 Ω) l Differential (A-B) output l Low noise and wide bandwidth
l Electrode
resistance test circuitry l Probe test circuitry l Driven guard shield
The FD223a is a dual differential, high impedance amplifier/electrometer
designed specifically for electrochemical measurements using ion specific
(K+, Na+, C1-, etc.) or pH glass microelectrodes.
The instrument is very stable, drift free, and features a built in provision
for measuring and adjusting input leakage current. DC levels may be
independently adjusted for each probe channel.
The ability to locate the sensing probes directly at the measurement
site overcomes the noise introduced by the long cables usually needed
to bring the measured potential to the instrument. Signal-driven guards at
FD223a Specifications
Input Impedance
> 1015 Ω, shunted by 0.5 pF
Input Capacitance
1 pF, nominal
Leakage Current
75 fA max
Gain
1.000 ± 0.1%
Output Resistance
50 Ω
Input Swing Voltage ±10 V
Rise Time (10 to 90%)
5 µs, small signal
#2547 Driven Guard Shield
Noise (0.1 Hz to 10 kHz) <100 µV p-p, input shorted
Baseline Stability
±0.1 mV/day
Position Controls Range ±600 mV
Physical Dimensions
Case: 8.8 x 21.0 x 17.5 cm (H x W x D)
Probe: 12.7 x 65 mm (D x L), 1.8 m cable
Power
90-265 VAC, 50/60 Hz, 10 VA
Probe Handle
6.5 x 65 mm (D x L)
Shipping Weight
2.5 kg
Operating Conditions Equipment is intended to be operated in
a controlled laboratory environment. Temperature: 0-40 °C; altitude:
sea level to 2000 m; relative humidity: 0-95%.
72
the probe input maintains the specified high resistance and reduces the
stray capacitance of the probes.
Careful design, coupled with quality component selection, particularly
in the headstage, results in an excellent amplifier with low noise and wide
bandwidth. The FD223a will faithfully reproduce the measured signal.
To reduce the noise and stray capacity even farther the probe housing
includes a signal driven guard. A portion of this inner driven shell is
exposed at the probe tip allowing a spring shield to be extended over the
electrode holder and microelectrode.
The amplifier features a probe test port that permits testing of the
electrode test feature and setting of the probe leakage current, (IG). A
standby mode is included and should be used when attaching glass
microelectrodes or electrode holders to the probe input. While in the
standby mode the voltage at the probe input is clamped near zero volts
thus protecting the input.
FD223A
FD223a Dual Channel Differential Electrometer
2 probes, driven guard shields and micropipette holder MEH1SF included for
all glass microelectrodes O.D. 1.0 mm, 1.2 mm, 1.5 mm, or 2.0 mm.
Optional Accessories
M3301LMicromanipulator (specify left- or right-handed)
M-3
80° Tilting base
RC1TReference cell (Ag/AgCl)
2547
Driven guard shield for FD223AP Probe
MEH1SFMicroelectrode holder
FD223APReplacement probe (includes calibration)
See cables and connectors, page 108
See microelectrode holders, page 120
See capillary glass, page 114
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
WPI’s Quietest Intracellular Amplifier!
Electro 705
BA
POWTTE RY
ERE
D
BLE
A
T
POR
Photo shows two units arranged for differential recording. Manipulators not included.
● Remote Headstage — Easily mounted in any manipulator, this
small probe, containing the first stage of amplification, includes a
microelectrode holder, which plugs directly into the probe input.
● Battery Power — Four 9V alkaline batteries (included) power the
Electro 705 for approximately 500 hours giving a super clean low noise
source of power making the Electro 705 the quietest amplifier available.
Batteries can be easily tested by the press of a button.
● Capacitance Compensation — Corrects for loss of rise time caused
by the presence of electrode capacity. Up to 50 pF of electrode shunt
capacity may be neutralized.
● Driven Guard Shield — Stray capacitance can be
further reduced by placing the driven guard shield
(included) over the microelectrode holder at the
input end of the probe.
ELECTRO 705 SPECIFICATIONS
Input Impedance10 Ohms, shunted by 1 pF
12
Output Impedance
100 Ohms, both outputs
Gain
X1: ±0.1%
Input Voltage Range
±5 V
Risetime
15 µs, 10-90%
Noise Level
500 µV peak-to-peak*
Input Capacitance Compensation 0-50 pF
Gate Leakage Current
±10 pA, adjustable to zero
Electrode Resistance Test
1 mV/ M Ohms
DC Positioning
± 300 mV
Common Mode Rejection>104 (in differential mode)
Power
Four 9V alkaline batteries, supplied
Dimensions
8.5 x 3.5 x 2.2 in. (22 x 9 x 6 cm)
Shipping Weight
5 lb (2.3 kg)
* Full band width, with 20 M Ohms source
● Tickler Circuit — A momentary oscillation that helps achieve cell
penetration.
● Electrode Resistance Test — The 705 provides a 1 nA electrode test
current. Electrode resistance is monitored at the 1X output as a voltage (1
mV/M).
● Probe Test Port — Allows the convenience of testing the amplifier's
intrinsic noise and gain without cumbersome external test hookups. Gate
leakage current can also be adjusted with minimum effort.
● Baseline Position Control — Adds or subtracts up to 300 mV to
the headstage output, allowing artifact voltages such as liquid junction
potentials to be nulled prior to recording.
● Differential Output — Two Electro 705s can be connected in tandem
to create an optional differential amplifier probe system.
SYS-705Electro 705 Electrometer
Probe, driven guard shield and micropipette holder MEH1SF included for
glass microelectrodes O.D. 1.0 mm, 1.2 mm, 1.5 mm, or 2.0 mm.
Optional Accessories
3468
Dual Rack Mount Kit
3469
Single Rack Mount Kit
M3301LMicromanipulator (specify left- or right-handed)
M-3
80° Tilting base
RC1TReference cell (Ag/AgCl)
2541
Driven guard shield for 705PF Probe
MEH1SFMicroelectrode holder
705PFReplacement probe (includes calibration)*
*Instrument must be returned to WPI for free calibration with new probe.
See cables and connectors, page 108
See microelectrode holders, page 120
See capillary glass, page 114
Reference
Koch, U. (2000) “Interdependence of spatial and temporal coding in the auditory midbrain.”
Journal of Neurophysiology 83, 4, 2300-2314
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
73
AMPLIFIERS, ELECTROMETERS
A low noise high quality intracellular
amplifier well-suited for the student lab
Duo 773 Dual Microprobe System
AMPLIFIERS, ELECTROMETERS
2-channel intracellular amplifier
For intracellular dual or differential studies, the Duo773 has separate negative capacity controls and built-in
active filtering that allows the precise balancing of time constants for artifact-free differential measurement.
Comes complete with two probe headstages, 1015 Ohms & 1011 Ohms probes to monitor signals from ionspecific micro-electrodes as well as KCl-filled electrodes.
* Although injected currents are “constant,” the
maximum current in a given situation will always
be limited by the system compliance of 10 V.
**The 712P headstage may be used on either
A or B channels, however Current Injection
specifications do not apply when used on channel
A. The 715P headstage may not be used on the
B channel.
References
L. Pluja (2000) “Electrical and mechanical
effects of vasoactive intestinal peptide and
pituitary adenylate cyclase-activating peptide
in the rat colon involve different mechanisms.”
European Journal of Pharmacology 389, 217224.
G. X. Wang, X. B. Zhou, et al. (2000) “Effects of
mitoxantrone on excitation-contraction coupling
in guinea pig ventricular myocytes.” Journal of
Pharmacology and Experimental Therapeutics
293, 2, 501-508.
S. Tsuruoka (2000) “Acute effect of cadmiummetallothionein on glucose and amino acid
transport across the apical membrane of the
rabbit proximal tubule perfused in vitro.” Journal
of Pharmacology and Experimental Therapeutics
292, 2, 769-777.
74
DUO 773 SPECIFICATIONS
HEADSTAGE (PROBE)
712P (red, port “B”)
715P (blue, port “A”)
ACTIVE PROBE INPUT IMPEDANCE
>1011Ω1015Ω
GAIN
x1, x10
x1
OUTPUT RESISTANCE
100 Ω100 Ω
OUTPUT VOLTAGE RANGE
±10 V
± 10V
MAXIMUM INPUT VOLTAGE
±15 V
±15 V
10-14 A
PROBE LEAKAGE CURRENT
5 X 10-12 A
DC POSITION ADJUST RANGE
± 300 mV
± 300 mV
ELECTRODE RESISTANCE TEST CURRENT
1 nA
1 pA, 1 nA selectable
INPUT CAPACITY COMPENSATION
+10 to -50 pF
0 to -10 pF
NOISE
Input shorted
<50 µV p-p 10kHz bandwidth
<50µV p-p 10kHz bandwidth
<200 µV p-p 10kHz bandwidth <200µV p-p 10kHz bandwidth
20 MΩ carbon resistor
RISE TIME
10-90% direct input small signal
1 µs, typical
25 µs, typical
10-90% through 20 MΩ (-C “on”)
CURRENT INJECTION
(712P only)**
Internal DC Current
± 50 nA low range, ± 500 nA high range
Externally commanded Current
± 500nA low range, ± 5 µA high range
External current command factor
20 mV/nA low range, 2 mV/nA high range
Current monitor
100 mV/nA low range, 10 mV/nA high range
Compliance
3V low range, 10V high range
Bridge balance
0-100 MΩ, 0-1000 MΩ
Bridge amplifier gain
x 10, x 50
LOW PASS FILTER
40 dB/decade, continuously variable 1-30 kHz
METER SECTION
Display
3.5-digit LED
Ranges
200 mV, 2000 mV, 20 V, 200 nA, 2000 nA
Accuracy and resolution
1 digit
DIMENSIONS
Instrument
17 x 5.25 x 10 in. (43 x 13 x 25 cm)
Probe
Diameter: 12 mm Length: 34 mm
POWER
95-135 V or 220-240 V, 50/60 Hz
SHIPPING WEIGHT
15 lb (7 kg)
CERTIFICATION
CE, CSA
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Headstage — Two gold-plated, epoxy sealed miniature active probes can
be positioned directly to the measurement site. Microelectrode holders
containing an Ag/AgCl electrochemical half-cells plug directly into the
probes. Stray capacitance can be reduced by placing the included driven
guard shield over the microelectrode holder at the end of the probe.
Capacity Compensation — Channel A can compensate up to 10 pF of
electrode shunt capacity and Channel B can compensate up to 50 pF.
Tickler Circuit — Assists in cell penetration. The frequency and
amplitude of the oscillations may be varied for differences in membrane
thickness or cell size. The duration of tickle can be controlled either by
using the momentary switch, a foot switch, or by applying a signal to the
remote tickler input.
Active Filters — Low pass settings on a -40 dB/decade active filter
vary the cutoff from 1 to 30 kHz. Either probe or bridge outputs may be
selected for filtering.
Current Injection — Channel B can eject current through the
microelectrode by applying a command signal to the stimulus input
connector; the resulting output from the probe will then be a constant
current replica of the input signal. Two ranges of current delivery are
provided: 50 nA and 500 nA or by an external source. This source can
be useful for delivering hyperpolarizing currents to stabilize the cell
membrane potential and as a holding current for microiontophoresis.
Compliance Alarm — When the electrode voltage exceeds the probe
input maximum allowed voltage, an audible over-compliance alarm will
sound.
Bridge Balance — Subtracts the excess electrode voltage associated
with delivering current through the recording micropipette. Electrode
resistances up to 1000 MΩ can be balanced in two ranges. The balanced
signal is available from x10 or x50 front panel output connectors.
Independent Outputs — The Duo773 has an output for each probe
independent of gain filtering or balancing. In addition the Duo773 has
a 10x and a 50x output for easy integration to most data aquisition
programs.
Digital Meter — The Duo773 comes complete with a 3½-digit display
for monitoring injection current or the voltages for either probe (single
ended or differential).
SYS-773
Duo 773 Electrometer
Specify line voltage
Includes two probes (712P and 715P or two 712P) with driven
guard shields and eight MEH1SF microelectrode holders for
1.0 mm, 1.2 mm, 1.5 mm, or 2.0 mm glass electrodes.
OPTIONAL ACCESSORIES
712PReplacement probe (includes calibration)*
715PReplacement probe (includes calibration)*
*Instrument should be returned to WPI for free calibration with new probe.
2933Rack Mount Kit, 51⁄4-in. high
2547
Driven Guard Shield for 712P & 715P Probes
15790Replacement Probe Handle
TW100F-4 Glass capillary with filament
TW150F-4 Glass capillary with filament
See Dri-Ref, page 68.
See cables and connectors, page 108.
AMPLIFIERS, ELECTROMETERS
#2547 Driven Guard Shield
Optional Holders
for Intracellular
Amplifiers
Typical setup:
Duo773
50x gain output
F
1S
EH
M
Data
Acquisition
System
2S
M
EH
2S
M
Recording Electrode
(with holder MEH1SF)
EH
F
FW
3S
Drug or dye current
electrophoresis injection
M
EH
3S
M
EH
Micromanipulator
EH
6S
M
EH
F
FW
6S
M
Micromanipulator
d)
lie
pp
(su
F
FW
See Microelectrode Holders,
Inverted Microscope
page 120
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
75
AMPLIFIERS, ELECTROMETERS
WPI’s Low-Noise Amplifiers
Outperform Cheap Imitations
A
n amplifier, in simplest
terms, is an electronic
device that magnifies
an input signal. However, the way
an amplifier is designed to handle
noise and bandwidth limitations
greatly affects the quality and
sustainability of the final output
signal.
reproduced. This is called
“hitting the rail.”
be generated
with the
amplifier. It is
determined by
the maximum
voltage of the power supply. If the amplitude
of the output signal is too large for the output
range, part of the signal is cut off (clipped).
• Rail – The upper or lower limit of the amplifier
range is called a rail. Signals that exceed the rail
cannot be faithfully reproduced.
Defining terms
To knowledgeably discuss amplifiers, let’s define
a few terms.
• Gain – The gain is the multiplier defining
how much the amplitude of an input signal
is increased. A signal with an ×1 gain is not
amplified. An ×10 gain produces an output
signal ten times greater than the input signal.
How does an amplifier work?
Power Supply Rails Limits the Range
• Noise – Any unwanted signal fluctuations
are called noise. While noise can also result
from external sources, for the purpose of this
discussion, we are primarily concerned with the
noise resulting from the inner workings of the
electronic device, our amplifier. This intrinsic
noise is called shot (or schott) noise.
In a perfect world an input signal can be
infinitely multiplied by the gain factor to
determine the output signal. For example:
• Signal to Noise Ratio (SNR) – The ratio of
the output signal to the noise of the amplifier is
called the signal to noise ratio. The smaller the
shot noise signal in an amplifier in comparison
with the output signal, the easier the desired
signal is to discriminate. When engineering an
amplifier, the SNR may be improved by boosting
the first stage gain to yield a larger output signal
or by using quality components to minimize the
shot noise level of the amplifier.
• Output Range – The output range
determines the maximum output signal that can
76
• DC Offset – DC offsets can appear in
biological preparations. This offset is the amount
the output signal is displaced away from a zero
reference point, and it is usually a result the
potential difference at the electrode’s tip.
Input Signal
Gain Output Signal
2mV ×1 2mV
2mV ×2 4mV
2mV ×10 20mV
2mV ×100200mV
2mV×10,000 20V
In the real world, however, the power supply
rails limit the possible output range of the
amplifier. For example, a bio-amplifier could
have a range of ±5.0V. In order for the output
signal to be faithfully reproduced, the input
signal times the gain factor must fall within
the voltage window set by the power rails.
Otherwise, the output signal will go off scale,
and the input signal will not be faithfully
In our example, a 1.0μV input
signal at an ×106 gain would generate a 1.0V
output signal. Since the power supply is rated up
to +5.0V, this output signal is clearly visible. If
the input signal in this example is greater than
5.0μV, the output signal would be greater than
+5.0V. Since 5.0V is the top of the range that
the power supply is capable of producing, the
output signal hits the upper rail and gets cut
off. This amplifier will give a +5.0V DC output
signal for all input signals greater than or equal
to 5.0μV. In this instance, a smaller gain factor
should be used to bring the output signal back
into the dynamic output range of the amplifier.
Noise Limits Amplifier Useability
All electronic devices produce their own internal
electronic noise, an unavoidable signal that can
mask the output signal. For example, if the input
signal is 2mV and the noise is 1mV, the signal
to noise ratio is two to one (2:1), and the output
signal would be undetectable. In this case, it is
nearly impossible to discern which part of the
output is generated by noise and which part is
the desired signal. (Fig. 1)
Signal vs. Noise
Range
{
+5.0V
Upper Rail
Signal
Noise
-5.0V
Lower Rail
Fig. 1–The higher the signal to noise ratio,
the more discernable the desired signal.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Ideally, the signal to noise ratio should be at
least 50 to 1 to produce a quality output signal.
A good signal to noise ratio can be achieved in
one of two ways:
Result of Amplifier Using Gain to Control Signal to Noise Ratio
+5.0V
+5.0V
+5.0V
-5.0V
-5.0V
-5.0V
• Boost the output signal by increasing the
gain.
• Reduce the noise.
While increasing the gain is the simplest
solution, too much gain can impose a limitation
on the dynamic range of the amplifier. Reducing
noise is a more complicated solution, but it
offers a greater range and more stability in the
end.
Output signal viewable in range
Two-Stage Amplifiers
signal to noise ratio.
Instead of using high gains in the first stage of
amplification, a well constructed bio-amplifier
that uses high quality components, like WPI’s
DAM series amplifiers, minimizes the noise
in the first stage of amplification so that the
dynamic range is retained throughout the
amplification process. A poorly designed
amplifier will simply increase the gain of the first
stage amplification until the desired signal to
noise ratio is reached.
Why not boost the power rails?
Stage 2 – The stage one output signal enters
the second stage of amplification where both
the signal and the noise from the first stage are
amplified together by the second stage gain
factor so that the signal is large enough to be
seen on a chart recorder or data acquisition
system. The second stage amplification is the
gain the user controls. It does not change the
Theoretically, increasing the voltage rails
powering the amplifier will increase the available
dynamic output range. It would seem natural
to increase the power supply rails coming into
the amplifier in order to provide the capability
for greater first stage gains. However, most data
acquisition systems are limited to a maximum
EMG
EKG
StimuMultiBattery
Isolated
lation
channel Powered
input signal ranging between ±10.0V. Therefore,
it is not practical to increase the power rails of
bio-amplifier beyond ±10.0V. Since the industry
standard limits us to ±10.0V power supply rails,
the only way to improve the signal to noise ratio
is to minimize the shot noise in the first stage of
amplification. This is why high quality amplifier
components are imperative.
Why does my signal flatline?
Regardless of the amplifier used, biological
potentials are often accompanied by a DC offset,
because the electrodes polarize over time. The
DC offset naturally increases over time. Since the
poorly constructed amplifier that utilizes greater
first stage gain has restricted its dynamic range,
it has limited ability to handle this offset. As the
offset continues to increase, the output signal
may eventually be forced by the offset into the
rail causing the flat line (clipping the signal). (See
Fig. 2.)
Connectors
Intracellular Bioamplifiers
DC
Electro 705
DC
Duo773
DC
t
2
t
t
t
t
WPI’s amplifiers
2 mm pin
t
2 mm pin
2
2 mm pin
4-8
Mini Banana or
8-pin DIN
t
Extracellular Bioamplifiers
ISODAM8A
DC
t
opt
t
ISO80
AC
t
t
t
DAM50
AC/DC
t
DAM80
AC
t
t
t
t
t
t
t
t
t
Mini Banana
t
RJ-11
t
Mini Banana
The amplifier that minimizes the
noise in the first stage amplification
offers a larger dynamic output range
and handles a much greater offset
value.
The purchase of a low-noise
amplifier pays dividends in the end.
WPI’s amplifiers were engineered for
the bio-medical researcher. While
20-30μV of noise is common in
bio-amplifiers, WPI’s DAM series
amplifiers generate 0.4μV RMS
(root mean squared) at 0.1-100Hz.
(That’s equal to 2μV peak-to-peak.)
The chart at left compares WPI’s bioamplifiers.
Transducer Amplifiers
BRIDGE8
DC
t
4-8
TBM4M
DC
t
4
8-pin DIN WPI
transducers
8-pin DIN WPI
transducers
Epithelial Voltage/Current Clamp Bio Amplifier
EVC4000
DC
t
1-4
Ussing 2 mm
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
77
AMPLIFIERS, ELECTROMETERS
Stage One – The unadulterated signal coming
into the amplifier is unaffected by the intrinsic
noise of the amplifier. Then, it runs through the
critical first stage of amplification where the
signal is boosted by the primary gain factor to
produce an output signal with the desired signal
to noise ratio. The intrinsic noise is not amplified
in the first stage. Higher gain factors used in the
first stage of amplification can seriously limit
the dynamic range available at output stage.
Large stage one gains also limit the gain factor
available in the second stage of amplification.
FD223A
Increasing offset renders the
signal useless
Fig. 2–As the offset naturally increases over time, a poorly constructed amplifier will not be able
to faithfully reproduce the signal. This offset can also be a result of gain drift which can occur as
the temperature rises.
Bio-amplifiers usually involve multiple stages of
amplification.
HeadAmplifier AC/DC Differential
stage
Offset causes the signal peak to
hit the upper rail
DAM Series Bioamplifiers
A family of very low noise battery-operated amplifiers
Now wi
th E S D P
• Gated or manual current generation for histological marking,
iontophoresis, or cell stimulation.
rotectio
n
!
•
A very low noise remote active headstage (DAM80 only) is
useful for very high impedance amplification utilizing
glass or metal electrodes.
Dam50—Basic Amplifier (optional
#5447 electrode adapter not included)
Optional probe #5489
for use with DAM50 also
in­cludes mi­cro­elec­trode
adapt­er #5469.
WPI’s DAM series amplifier’s are well known as a standard of the
industry for extracellular potential amplification. These battery powered
bio-amplifiers are designed with a compact chassis profile that enables
the user to locate the unit closer to the preparation and thereby
minimize long lead lengths which contribute to noise. Each amplifier
is equipped with selectable high and low filters, and a position
control to offset galvanic potentials which may develop during
recording. A choice of models offer additional features that are useful
for certain applications:
Application Examples
DAM50
#5447 Adapter
DAM50
GND
A
GND
#5371 Cable
B
Electrode Adapter
#5371 Cable
#3294 Grounding Clip
#5489 Probe
Light
Stimulation
#300102 electrode
extension, held by
micromanipulators
#2033 Adapter
B
#3578 Cable
A
AMPLIFIERS, ELECTROMETERS
DAM series amplifiers can be used as standalone
units on any tabletop, or use optional clampmounting hardware to locate them conveniently
within the work area. Alternatively, a pair of
amplifiers can be mounted into a standard
equipment rack with a rack mount kit (#3484 ).
A variety of hook up accessories are available to
configure your application.
Micromanipulator
Metal Microelectrodes
#5469 Metal
Microelectrode
Adapter
Central Ground
Metal Microelectrode
(i.e., TM33B01)
Petri Dish
Immobilized specimen
EP2
Ag/AgCl Half Cell
Ag/AgCl pellet
Central Ground
Extracellular recording
using metal microelectrode
78
ERG recording of fly eyes
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
DAM SERIES SPECIFICATIONS
Dam80—
With low-noise
headstage DAM80P
#3294 Grounding Clip (included)
DAM80i Probe (included)
#5371 Cable
A
B
#13388 Adapter
#300102 electrode
extension, held by
micromanipulators
SYS-DAM50
SYS-DAM80
INPUT LEAKAGE CURRENT
50 pA (typical)
MAX. DC DIFFERENTIAL SIGNAL
± 2.5 V (DAM 50)
GAIN
AC: 100x, 1000x, 10000x
DC: 10x, 100x, 1000x (DAM50)
COMMON MODE REJECTION RATIO
100 dB @ 50/60 Hz
INPUT CAPACITANCE
20 pF
AC MODE NOISE
AC MODE NOISE
DC MODE NOISE (DAM50)
0.4 µV RMS (2 µV p-p) 0.1-100 Hz
2.6 µV RMS (10 µV p-p) 1 Hz-10 kH
7.5 µV RMS (30 µV p-p) 3-10 kHz
Metal Microelectrodes
Ag/AgCl skin electrode pad
(#EL203)
AC Mode
AC Mode (DAM80)
DC Mode (DAM50)
Low frequency, 0.1, 1, 10, 300 Hz
High frequency, 0.1, 1, 3, 10 kHz
High frequency, 0.1, 1, 3, 10 kHz
OUTPUT CONNECTORS
BNC on DAM50; 3.5 mm MiniPhone connector on DAM80
OUTPUT VOLTAGE SWING
±8 V
OUTPUT IMPEDANCE
470 Ω
BATTERY TEST
Audible tone
CALIBRATOR SIGNAL
10 Hz square wave
POSITION
Approximately 250 mV
CURRENT SOURCE
DAM80: DC Generator
0 to ±50 µA, variable
EXTERNAL COMMAND
Input Voltage ±10 V commands
AC or DC current waveform
±50 µA max. amplitude @ 200 KΩ
BATTERIES
2 x 9V alkaline (included)
DIMENSIONS
DAM50
DAM80
8 x 4 x 1.75 in. (20.3 x 10.2 x 4.4 cm)
7 x 4 x 1.75 in. (17.8 x 10.2 x 4.4 cm)
SHIPPING WEIGHT
3.5 lb (1.6 kg)
FEATURE
Input Mode
Input configuration
Gain Range
High / Low Filters
Offset position control
Current Generator
Remote Active headstage
Output connection
Standard input connection*
Power supply
DAM50 DAM80
AC/DC
AC
differential/single ended
differential
100-10K (AC), 10-1K (DC)
100-10K (AC)
yes
yes
yes
yes
NoYes
NoYes
BNC
3.5 mm mini phone
unterminated wire
mini banana
(2) nine volt alkaline batteries
(2) nine volt alkaline batteries
*see optional accessories for additional alternatives
Bio-amplifier
Bio-amplifier with active probe (DAM80P)
OPTIONAL ACCESSORIES
DAM80PReplacement Probe
3072
6 Replacement Modular Cables (DAM50)
3517
2 Optional Shielded Modular Cables (DAM50)
CBL102
3.5 mm Phone plug-to-BNC Cable
2851
BNC-to-BNC Cable
2033
Black Insulated Mini-Banana Plug
2034Red Insulated Mini-Banana Plug
2035Uninsulated Mini-Banana Plug
2101
9V Alkaline Battery, each (2 required)
3484Rack Mount Kit (for 2 DAM preamps)
3485Ringstand Mounting Kit
5447Electrode Adapter (DAM50)
5469Metal Microelectrode Adapter for DAM80
(mini-banana plug to 0.031 in. (0.79 mm) socket)
5489
Adapter for Metal Microelectrode (DAM50)
13388
Adapter, mini-banana plug to 2mm socket
5371
Cable, Low Noise (2 mm pin to 2 mm pin)
3578
Adapter Cable for Ag/AgCl pellets (2 mm pin)
300102Electrode Extension, 4-inch
3414
9V NiMH Battery
Also see cables and connectors, metal mi­cro­elec­trodes,
carbon-filled mi­cropi­pettes.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
79
AMPLIFIERS, ELECTROMETERS
Included with the DAM-80 is a Startup Kit containing the following
accessories needed for basic metal electrode electrophysiology research:
CBL102 Cable, BNC-to-3.5mm plug, 6 ft (2m) (two)
5469 Adapter, mini-banana to 0.031 skt. (two)
13388 Adapter, mini-banana to 2mm skt. (two)
3294 Cable, ground clip to wire, 3 ft
2033 Mini-banana plug, black
2034 Mini-banana plug, red
2035 Mini-banana plug solderable turrent (two)
EP1 Ag/AgCl pellet (70 mm wire) 1mm diam x 2.5 mm long
M3301EH Electrode Holder, 14cm (two)
5470 0.031-inch jack on 12-inch wire (package of 4)
EMG
recording
of a rat
1012 Ω, common mode and differential
BANDWIDTH FILTER SETTINGS
DAM80, an AC amplifier only, features a very low noise headstage
probe which can be mounted in mi­cro­ma­n­ip­u­la­tors for up-close cortical
recording, for ex­tra­cel­lu­lar recording from high impedance glass or metal
mi­cro­elec­trodes. Also provides a gated cur­rent for tissue marking. Mi­cro­
elec­trode holder MEH3SB is recommended.
DAM80 or ISO-80
INPUT IMPEDANCE
AMPLIFIERS, ELECTROMETERS
Isolated
Differential
Amplifier
The improved ISO-80 provides low noise AC
coupled amplification and offers excellent
recording performance for monitoring
extracellular nerve action potentials in
vitro and in living animals. The ISO-80 is
provided with a remote headstage (1 m cable)
which incorporates an electrode impedance test
function and a constant current stimulator. The constant
current stimulator can be used for cell marking, stimulation or electrode
cleaning. Typical applications include measuring EMG, EEG, extracellular
and action potentials in vitro or in vivo. The ISO-80 system is DC isolated
from the subject ground and employs state of the art electro-magnetic
shielding for improved noise rejection. The amplifier employs both high
pass and low pass filtering with gain from 100 to 10,000. The lowest lowpass setting is 5Hz and the upper passband is 10 kHz.
Included with the ISO-80 is a Startup Kit containing the following
accessories needed for basic metal electrode electrophysiology research:
CBL102 Cable, BNC-to-3.5mm plug, 6 ft (2m) (two)
5469 Adapter, mini-banana to 0.031 skt. (two)
13388 Adapter, mini-banana to 2mm skt. (two)
3294 Cable, ground clip to wire, 3 ft
2033 Mini-banana plug, black
2034 Mini-banana plug, red
2035 Mini-banana plug solderable turrent (two)
EP1 Ag/AgCl pellet (70 mm wire) 1mm diam x 2.5 mm long
M3301EH Electrode Holder, 14cm (two)
5470 0.031-inch jack on 12-inch wire (package of 4)
ISO-80 Specifications
Input Resistance
>1011 Ohms, Common Mode and differential
Input Leakage Current
50 picoamperes, max.
Amplification
×102, ×103, ×104
Common Mode Rejection Ratio 100 dB typ. @ 50/60 Hz
Equivalent Noise Signal Input 0.4 microvolts rms (0.1-100 Hz)
2.0 microvolts rms (1 Hz - 10 kHz)
Filter Settings
Low frequency
High frequency
5, 10, 100, 300 Hz
100 Hz, 1, 3, 10 kHz
Max. Output Voltage Swing
±8 volts
Electrode Impedance Range
100 kOhm - 10 MOhm @ 300 Hz
Stimulation Current
0 to ±20 micro amperes (constant current)
ISO-80
Isolated Bioamplifier w/ active probe (ISO80P)
Specify line voltage
OPTIONAL ACCESSORIES
ISO-80PReplacement ISO-80 Probe
CBL102
3.5 mm phone plug-to-BNC cable
Maximum STIMULATION Voltage ±15 volts
Maximum Electrode Voltage
±40 volts
Display31⁄2-digit LCD
BATTERY TEST
Low battery display
Power
Two 9-volt NiCad batteries & charger,
supplied
SHIPPING WEIGHT
4 lb (1.8 kg)
To ISO-80
To ISO-80
To ISO-80
ISO-80 probe
ISO-80 probe
#5470
electrode
adapter*
ISO-80 probe*
red socket
#5469 electrode adapters*
micromanipulator
#5469 electrode adapter*
micromanipulator
metal microelectrodes
metal microelectrode
specimen
#3294 grounding
wire & clip*
Cut, strip, and secure
the wires in #2033
connector.
#3294 grounding
wire & clip*
EP1* or similar
Ag/AgCl half-cell
#2033
connector*
Differential Application
80
micromanipulator
specimen
EP1* or similar
Ag/AgCl half-cell
red
socket
#3294 grounding
wire & clip*
Single-Ended Application
micromanipulator
#2033 connector*
specimen
#M3301
electrode
holder*
metal
#5469 microelectrode
electrode
adapter*
EP1* or similar
Ag/AgCl half-cell
Optional Differential Application
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Dual Microiontophoresis Window
Current Generator
Discriminator
● Monitor signals and discriminator levels
simultaneously at the multiplex output
● Window height independent of lower discriminator
level setting
● Logic level output pulses, TTL compatible
● Output pulses indicated by LED display
The Dual Microiontophoresis Current Generator (Model 260) is an
electrically isolated, battery-operated instrument designed for the electroiontophoresis of dyes, drugs and charged substances from micropipettes.
Two identical battery operated current generators are available. In
ordinary use, the two current generators are operated in parallel providing
two distinct currents; one for preventing substances in the micropipette
from outward diffusion (the retain or hold current) and the second for
actively ejecting charged material. For pipettes with submicron tips, a hold
current may not be necessary if there is little outward diffusion of pipette
material. Model 260 is powered by two 9-volt alkaline batteries per side
(four, in total); unique circuitry converts the ±9 V to ±100 V without a
transformer, yielding an exceedingly quiet output.
Ωmega-Tip-Z
™
Audible Baseline Monitor
The ABM, a bat­tery-op­er­at­ed volt­age-con­
trolled os­cil­la­tor, lets you monitor po­ten­tial
au­di­bly. ABM is par­tic­u­lar­ly use­ful be­cause
it allows the user to es­ti­mate volt­age lev­els
when us­ing a mi­cro­scope or en­gaged in oth­er
tasks which do not al­low viewing an os­cil­lo­
scope or re­cord­er. An audible tone is gen­er­at­
ed with increasing pitch as the input volt­age
becomes in­creas­ing­ly positive.
SYS-ABM
Audible Baseline Monitor
Low-Pass Filter
Upper frequency band limit
can be smoothly varied
between 100 Hz and
30‑kHz using a single knob.
WPI’s new low-pass filter is
small, con­sumes little power,
and can be located almost
anywhere in your workspace. Features switch-selectable gain of ×1 or
×10 and low noise. An ideal tool for filtering an analog signal before
digital conversion by data acquisition systems.
SYS-LPF30
Lo-Pass Filter
Millivolt and megohm meter measures impedance of metal or glass capillary microelectrodes
Omega-Tip-Z was created especially for measuring impedance in
etched tung­sten, platinum-iridium* and steel microelectrodes, as well as
electrolyte-filled micropipettes. The meter’s AC impedance-measuring
circuit is unaffected by electrode
offset or tip junction potentials. The
gold-plated miniature probe lets you
conveniently monitor micro-electrode
impedance in electrolytes, and an
electrode tip cleaning feature lets you
remove buildup quickly. Omega-Tip-Z
can also measure DC electrode tip
potentials up to 2000 millivolts. The
instrument operates for hundreds of
hours without battery failure.
NOTE: Metal microelectrodes which
have been precalibrated at 1 kHz
should be baselined for use with
Omega-Tip-Z.
*See Metal Microelectrodes,
page 88.
SYS-OMEGAZ Omega-Tip-Z with Probe & Holder
711PReplacement Probe
5468
Adapter to connect metal microelectrodes to
probe, 2 mm socket to .031 in. receptacle OPTIONAL ACCESSORIES
Z-LITE
Fiber Optic Illuminator (115v, 60Hz, beige case)
Z-LITE-Z
Fiber Optic Illuminator (230v, 80Hz, black case)
500186
Bifurcated Light Guide with lenses
Z-LITE-186
Z-Lite Illuminator and bifurcated light guide
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
81
AMPLIFIERS, ELECTROMETERS
SYS-260
Dual Microiontophoresis Current Generator
OPTIONAL ACCESSORIES
2933Rack Mount Kit, 51⁄4-in. high
SYS-121
Window Discriminator
2932Rack Mount Kit, 3.5-in. High
2851
BNC-to-BNC cable, 5’2”
500184
BNC-to-BNC cable, 10 ft
500257
BNC-to-BNC cable, 6 inch 15 cm
500258
BNC-to-BNC cable, 12 inch 30 cm
500259
BNC-to-BNC cable, 18 inch 46 cm
Choose the amplifier modules you need to measure nearly anything!
l Rack mountable and stackable
l Ergonomic design
l 8-Channel
l Small footprint
AMPLIFIERS, ELECTROMETERS
Signal conditioning in the last decade has
increasingly moved in the direction of the
computer, because the software preserves the
raw data and is highly reliable. Operations such
as integration, differentiation, filtration and
even waveform generation are now efficiently
handled in software. On the other hand,
however, the transduction of physical signals
such as bio-potentials, force, temperature,
pressure or ionic concentrations must be
measured with an electronic amplifier.
SIH/WPI’s physiology amplifier system
focuses on this idea and provides a flexible
electronic platform intended to process the
transduction of physical signals, displacement
transducer outputs and the outputs from
electrochemical free radical sensors. This
platform simply focuses on the reliable
transduction of the electronic signal and
provides a convenient passage for the
translation of real world signals to a computer
for analysis.
The system consists of an 8-channel, rack
mountable frame that includes an ultra quiet,
shielded power supply. All of the module
outputs are routed to rear panel connectors. If
you prefer, outputs may be routed internally to
the inputs of other modules. The system has a
small footprint and may be stacked to provide
as many channels as you need.
SI-BMFA
82
8-Channel Enclosure
The SI-BMFA Power Frame is the
foundation of the SI Heidelberg
modular physiology suite. It
incorporates a robust power supply
that can accommodate up to
eight physiology modules, which
can be mixed or matched in any
combination. Modules are quick
and easy to install, thanks to an
innovative and mechanically solid
card rail system.
Features
l 8 channel slots
l Backplane design includes
provision for configurable
communication between modules
Power Frame Enclosure
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Optical Transducer Amp
Bridge Amplifier
The SI-BRIDGE80 module is
designed for use with standard bridge
type transducers to measure pressure,
force, or displacement.
Features
Features
l Rapid Auto Zeroing function with
fine offset adjustment
l Multiple gain ranges from 1X to
1000X plus an adjustable gain
option
l Offset indicator LED’s
l Multiple gain ranges with
adjustable fine tuning for precise
calibration
l Defeatable offset adjustment
control
l Digital interface for optional
Anti-Oscillation module for use in
isotonic studies
l Low pass filter down to 30 Hz
SI-BAM21-LCB Optical Transducer Amplifier
SI-BRIDGE80
l Operates in differential or single
ended configuration
Bridge Amplifier
SI-BRIDGE80 SPECIFICATIONS
Input Impedance
> 1012 Ohms
Power
12 VDC provided by chassis
Amplification
1, 10, 50, 100, 500, 1000 and Adjustable
Gain1×, 2×, 5×, 10× Optional factory setting: 10×, 20×, 50×, 100×
Input Leakage Current 0.1 pA at 25 °C
Voltage Offset Adjustment
± 50 mV (low); ± 100 mV (high)
Input Offset Adjustment
± 2.0 VDC
Amplifier Output Voltage
± 4.4 V (10 mA max.)
Output Impedance
470 Ω
Excitation Voltage
10 V (± 5.0V) 100 mA max
Power
12 VDC provided by chassis
Equivalent Noise Signal Input
< 0.4 μV RMS (2 μV p-p) 0.1–10 Hz Gain >10 Output Range
± 10 VDC
< 3 μV RMS (15 μV p-p) 0.1–100 Hz Gain >10
Low Pass Filter Band (KHz)
0.03, 0.1, 0.3, 1, 5, 10, Wide Band R-C Butterworth 6 dB / Octave
SI-BAM21-LCB SPECIFICATIONS
Input Configuration
Current to voltage converter
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
83
AMPLIFIERS, ELECTROMETERS
The SI-BAM21-LCB is designed
specifically for use with the
SI Heidelberg KG series force
transducers. Also available in a single
standalone enclosure (see page 6),
either version provides an incredibly
quiet, linear and stable transducer
signal to your data recording system.
AMPLIFIERS, ELECTROMETERS
Extracellular Bio-Amp
Anti-Oscillation Unit
The SI-DAM800 is a general purpose
extracellular bio-amplifier module
with an optional remote head stage,
useful for working with metal micro
electrodes.
The SI-AOSUB Anti Oscillation
module operates in conjunction
with the SI-BAM21-LCB module
and the motor controller to actively
cancel the force transducer’s natural
harmonic resonance. This is critical
when performing dynamic muscle
contraction and release studies to
ensure the integrity of the force signal
from the tissue.
Features
l High and low pass filters
lMultiple gain ranges from 10X to
10,000X
l AC or DC coupling
Features
l Optional remote powered
headstage
l Adjustable amplitude and
frequency of pulse generation to
ping transducer resonant frequency
l LED amplitude meter peaks when
resonant frequency is generated
SI-AOSUB
Anti-Oscillation Module
SI-DAM800
Extracellular Bio-Amplifier
SI-DAM800 SPECIFICATIONS
Power
Input Impedance
Input Leakage Current Max. DC Differential Signal
Gain (AC or DC)
CMMR
Input Capacitance
AC Mode Noise
AC Mode Noise
DC Mode Noise
Bandwidth Filter Settings
Output Connector
Output voltage
Output Impedance Calibrator Signal Position
DC Current Source Gen.
External Commands
AC / DC Current Waveforms
84
12 VDC provided by chassis
> 1012 Ω common mode and differential
50 pA at 25 °C typical
± 2.5 V
10, 100, 1000, 10000
100 dB @ 50/60 Hz
20 pF
0.4 μV RMS (2 μV p-p) 0.1–100 Hz
2.6 μV RMS (10 μV p-p) 1 Hz–10 KHz
7.5 μV RMS (30 μV p-p) 3–10 KHz
Low Filter Frequency: 0.1, 1, 10, 300 Hz
High Filter Frequency: 100 Hz, 1, 3, 10 KHz
3.5 mm MiniPhone connector
± 8V
470 Ω
10 Hz square wave
250 mV approx.
0 - ± 50 μA variable
± 10V signal levels
± 50 μA max. amplitude at 200 K Ω
SI-AOSUB SPECIFICATIONS
Power
12 VDC provided by chassis
Input ± 10 VDC
Pulser Output
0 – 10 VDC adjustable
0.1 Hz – 4.0 KHz
Damping Frequency Range
0.1 Hz – 2.0 KHz
Output Range
± 10 VDC
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Temperature Control
AMPLIFIERS, ELECTROMETERS
The SI-H Temperature Control Unit is designed for use with the SI-H line of
muscle physiology research platforms. It maintains the temperature of an
SI-H cuvette up to 45ºC. It is accurate to 0.1ºC. The circuit is appropriate
to RTD (resistive temperature device) applications. It linearly converts a
temperature reading to a voltage that is displayed as a temperature on the
SI-TCM2 and can be recorded. This unit is available in a standalone model
and as a module for the Signal Conditioning Amplifier System backplane.
Features
l Controls two cuvettes simultaneously
l Uses PID control to maintain a constant temperature with ±0.1ºC
tolerance
l Has both high and low alarm warnings which can be user defined
l Easy to control with simple interface
l Also available as a stand-alone device ­— call for details and price.
SI-TCM2
Two-Channel Temperature Control Module
SI-TCM2 SPECIFICATIONS
Input Configuration
Current to voltage converter
Power Requirements
12V DC at 2.5A 50/60Hz wall adaptor, 2.5mm ID/5.5mm OD with positive center DC barrel (included–WPI #801513)
Operating Temperature RangeRoom temperature
Display Precision0.1ºC
Controller Resolution0.1ºC
Cuvette Temperature Sensor1000Ω RTD (1000Ω at 0ºC)
New Cell Tester
see page 3
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
85
These transducers are
designed for general use
in data acquisition and
recording instruments and
systems. They are supplied
with 5-ft cables terminating
in an 8-pin DIN connector
which readily plugs into
Transbridge, a 4­-channel
analog signal conditioner
and Bridge8 .
LUME
AMPLIFIERS, ELECTROMETERS
PNEU
LIGHT
PRESSURE
When powered by +2 volts or more, LUME provides an output voltage
sig­nal pro­por­tion­al to inci­dent light over the visible to near infra red
spectrum. Contain­ing a min­ia­ture silicon pho­to­diode, the slender stainless
steel probe can be easily mounted to do a wide va­ri­ety of useful jobs,
such as mon­i­tor­ing trans­mit­ted, reflected or in­ter­rupt­ed light beams. Optic
fi­bers up to 2 mm di­am­e­ter may be in­sert­ed into the tip.
PNEU transducers are ac­cu­rate dif­fer­en­tial pressure mea­sur­ing de­vic­es for
noncorrosive gas­es and gaseous fluids. Lin­ear and re­peat­able, with little
tem­per­a­ture drift, they con­vert hy­dro­stat­ic pressure levels to sta­ble and
proportional voltage sig­nals.
LUME Photocell
3491Probe Extension Cable
Caution: Extension cable may diminish signal-to-noise ratio.
PNEU01
Pressure Sensor, 1 psig
PNEU05Pressure Sensor, 5 psig
PNEU15Pressure Sensor, 15 psig
3491Probe Extension Cable
Caution: Extension cable may diminish signal-to-noise ratio.
PNEU01, PNEU05, PNEU15 SPEC­I­FI­CA­TIONS
LUME SPECIFICATIONS
WORKING PRESSURE
POSITIVE SUPPLY VOLTAGE
+50 V, maximum
NEGATIVE SUPPLY VOLTAGE
-20 V, maximum
LIGHT VOLTAGE
50 mV minimum (per 5 mW/cm2 with 2870°K source)
DARK OUTPUT VOLTAGE
100 µV max. (+ 5 V supply)
ANGULAR FIELD OF VIEW
± 12 degrees off axis
SPECTRAL RANGE
400 - 1200 nm (peak at 860 nm)
PROBE
5 mm diam. x 100 mm, PNEU01
0-1 psi, differential (52 mm Hg)
PNEU05
0-5 psi, differential (260 mm Hg)
PNEU15
0-15 psi, differential (780 mm Hg)
MAXIMUM PRESSURE
3x Full Scale Pressure
TRANSDUCER SENSITIVITYPNEU01: 18 mV, Full Scale (±10% at 10 V)
PNEU05: 60 mV, Full Scale (±10% at 10 V)
PNEU15: 90 mV, Full Scale (±10% at 10 V)
SUPPLY VOLTAGE
10 V (±5 V)
INPUT & OUTPUT RESISTANCE
4 kΩ, nominal
LINEARITY ERROR
0.5% maximum
stainless steel
KG Optical Force Transducers
●● Different models to
accommodate a wide
range of forces and
sensitivities
●● Nearly insensitive to
changes in temperature
and ambient light
●● Extremely high level of
linearity
●● Virtually indestructible
with normal use
●● Lifetime warranty
●● Simple calibration
●● KG transducers are for use with WPI’s BAM21-type amplifier
86
Transducer specifications
Unloaded transducer without tissue mounting support
Force
Range
SI-KG2
SI-KG2A
SI-KG2B
SI-KG4
SI-KG4A
SI-KG7A
SI-KG7B
SI-KGxx
Range
Noise
Compliance Resonance
(nm/mN)
Frequency
0-2 N
0-200 g 250 µN
150
1.3 kHz
0-0.5 N
0-50 g 300 µN
0-0.2 N
0-20 g
80 µN
590 Hz
0-50 mN
0-5 g
15 µN
0.5
1.2 kHz
0-20 mN
0-2 g
4 µN
1
1.2 kHz
0-5 mN
0-0.5 g
0.4 µN
5
500 Hz
0-10 mN
0-1.0 g
1 µN
1.5
550 Hz
contact us for specialty transducers with different ranges.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
FORT
5000
FORT
FORCE TRANSDUCERS
1000
FORT
These rigid-lever force transducers transform applied force into pro­por­
tion­al voltage. Using balanced strain gauges, FORT transducers produce
lin­ear output volt­age vs. applied force input with very little deflection.
To use, clamp the handle of the FORT transducer in a horizontal
position and apply the forces to be mea­sured to a rivet or hook mounted
in the hole at the end of the flat sensing leaf.
250
FORT
100
FORT100
FORT250
FORT1000
FORT5000
Force Transducer (100 g)
Force Transducer (250 g)
Force Transducer (1000 g)
Force Transducer (5000 g)
FORT SPECIFICATIONS
FORT250
FORT1000
FORT5000
100 grams
250 grams
1000 grams
5000 grams
OUTPUT SENSITIVITY (± 10%)
7 µV/V/g
3 µV/V/g
0.84 µV/V/g
0.38 µV/V/g
INPUT & OUTPUT RESISTANCE
350 Ω
350 Ω
350 Ω
350 Ω
RESOLUTION
0.01% of full scale force
0.01% of full scale force
0.01% of full scale force
0.1% of full scale force
RESONANT FREQUENCY
300 Hz
300 Hz
300 Hz
60 Hz
LINEARITY ERROR
Less than 0.1% of full scale
Less than 0.1% of full scale
Less than 0.1% of full scale
Less than 0.1% of full scale
MAX. OPERATING VOLTAGE
10 V AC or DC
10 V AC or DC
10 V AC or DC
10 V AC or DC
MAXIMUM APPLIED FORCE
3× rated full scale force
3× rated full scale force
3× rated full scale force
3× rated full scale force
DRIFT
thermally compensated
thermally compensated
thermally compensated
thermally compensated
DIMENSIONS
0.3 inch diam × 4 in.
(7.6 mm diam × 10.2 mm)
0.3 inch diam × 4 in.
(7.6 mm diam × 10.2 mm)
0.3 inch diam × 4 in.
(7.6 mm diam × 10.2 mm)
0.3 inch diam × 4 in.
(7.6 mm diam × 10.2 mm)
WEIGHT (excluding cable)
0.3 oz (8 g)
0.3 oz (8 g)
0.3 oz (8 g)
0.3 oz (8 g)
10g & 25 Force
Transducers
FORT10g
FORT25
Force Range, full scale
0-10 grams
0-25 grams
Output Sensitivity
10 mV/gm, nominal
3 mV/gm, nominal
INPUT & OUTPUT ResistANCE
1500 Ω
1500 Ω
Resolution
< 1mg
< 2mg
Resonant Frequency
450 Hz
450 Hz
Linearity Error
<0.2% of full scale
<0.2% of full scale
Maximum Operating Voltage
10 V DC (-5V ~ +5V or 0 ~ 10V)
10 V DC (-5V ~ +5V or 0 ~ 10V)
MAXIMUM APPLIED FORCE
2× rated full scale force
3× rated full scale force
DRIFT
<30 mg/hr
<50 mg/hr
DIMENSIONS
40 x 22 x 19 mm
40 x 22 x 19 mm
Handle 88 mm
Handle 109 mm
100 gram
100 gram
Weight
These 10-gram and 25-gram force
transducers are reliable tools for high
precision force measurement. Using balanced
semiconductor strain gauges, both produce
linear output voltage vs. applied force input
with very little deflection. The rigid lever force
transducer transforms the applied force into a
proportional voltage. Featuring a temperaturecompensated full-bridge configuration with
four high sensitivity semiconductor strain
gauges. These transducers have broad
dynamic measuring range and very high
sensitivity.
To use, clamp the handle of the FORT10
or FORT25 transducer in a horizontal
position and apply the forces to be measured
to a rivet or hook mounted in the hole at the
end of the flat sensing leaf.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
87
AMPLIFIERS, ELECTROMETERS
FORT100
FORCE RANGES, FULL SCALE
Metal Microelectrodes
Superior microelectrodes for outstanding extracellular recording — tungsten, iridium,
platinum-iridium, and Elgiloy®
EXPOSED TIP DIMENSIONS
Metal
(nominal)
NominalPlatinumPure
ImpedanceTungsten
ElgiloyIridiumIridium
10 kΩ250 µ
_________
50 kΩ200 µ
_________
0.1 MΩ100 µ120 µ60 µ45 µ
0.5 MΩ55 µ66 µ18 µ14 µ
1.0 MΩ30 µ36 µ10 µ10 µ
2.0 MΩ12 µ15 µ6 µ5 µ
5.0 MegΩ5 µ6 µ3 µ2.5 µ
Gold-plated connector pin
Parylene coating
Kapton* tubing, indicated by “KT” in
the part number, extends from the
con­nec­tor to within 5 mm of the tip,
pro­vid­ing stiffness and additional
in­su­la­tion to the electrode shaft.
Kapton-clad elec­trodes are rec­om­
mend­ed when the electrode is to be
inserted through a cannula for extra
deep pen­e­tra­tion.
Exposed tip
25:1 taper
Parylene-C*
Gold-plated connector pins
Shrink tubing
2-, 3-, 5-inch lengths
Polyimide tubing
0.014" O.D. for 0.005" O.D. WE
Type A
Glue
Parylene-C
Insulation
Tip separation
90 or 125µm ±15%
5 mm
typical
3" and 5" total length
Note: Electrode diagrams not shown to scale.
Exposed
tips
Type B
Gold-plated connector pin
Polyimide Tubing
2-, 3-, 5-inch lengths
AMPLIFIERS, ELECTROMETERS
Parylene coating
Type C
core conductor
Concentric Bipolar Electrodes
exposed stainless steel surface
stainless steel tubing
Excellent for shielded macro recording as well as evoked
potentials — especially well suited for bipolar stimulation
The tungsten electrode is sharpened to a point and is 75 microns in
diameter. The outer stainless steel conductor is insulated with Polyimide
tubing to within 0.2 mm of the end of the stainless steel tube. Also
available without the outer Polyimide insulation.
Heat Treated Tip
(above) is ideal for
penetrating tough
membranes (not
recommended
for chronic
implantation). This process is performed using a microforge in which the
heating element is positioned in close proximity to the tip in order to melt the
Parylene-C distal to the exposed metal. It provides a smooth transition and
produces better adherence of the Parylene-C to the metal.
Polyimide tubing
x
shrink tubing
Y <0.2 mm
Y
L=3" or 5" (2" for PTM)
Insulated metal conductor with exposed concentric surface
.389"
(9.88 mm)
.031"
(0.79 mm)
pin #5482
.404"
(10.26 mm)
socket #5483
To have your electrodes heat treated, just add the suffix “H” to any of the “KT”
numbers on the facing page. Cost of the treatment is $10 per package of 10
electrodes ($20 per package for Type B).
Gold-plated pins (#5482)
and sockets (#5483) may
be attached to 24-, 26-, or
28-gauge wire.
0.51 mm ID
#5470 (mount in M3301EH
holder, see page 200)
* Parylene is a trade mark of Union Carbide. Kapton is a trade mark of DuPont. Elgiloy is a trade mark
of Elgiloy Ltd.
ACCESSORIES
300102Micromanipulator holder, 4 in., 2mm to 0.031 socket
5468
2 mm receptacle to 0.031-inch jack (for Omega-TipZ)
5469
Adapts mini banana plug (DAM80) to 0.031-inch receptacle (metal microelectrode)
5470
0.031-inch jack, 28 ga. wire, 12 inch (pkg of 4)
5482*Pins, 0.031-inch, gold-plated (pkg of 50)
5483*
Sockets, 0.031-inch gold-plated (pkg of 50)
*Gold-plated pins (#5482) and sockets (#5483) may be attached to 24-, 26-, or
28-gauge wire.
88
Electrode
plugged into
adapter #5470
#5468
#300102
#5469
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
W
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Introductory Assortments
Each of these assortment kits includes electrodes with different impedance within each style. Use an assortment kit to determine which electrode you need for
your experiment. Ten electrodes per box, no mixing.
Item
TM31/33Axx
TM31/33AxxKT
TM33BxxKT
TST33AxKT
Contains the following electrode impedances by quantity (pkg of 10)
TM33A05 (2), TM33A10 (3), TM33A20 (3), TM31A50 (2)
TM33A05KT (2), TM33A10KT (3), TM33A20KT (3), TM31A50KT (2)
TM33B01KT (3), TM33B05KT (2), TM33B10KT (3), TM33B20KT (2)
TST33A05KT (3), TST33A10KT (4), TST33A20KT (3)
Concentric Electrodes*
Item
Metal Core Length
Imp
Tungsten
3” (76)
10-15K
TM33CCNON
TM33CCINS
Tungsten
3” (76)
10-15K
TM53CCINS
Tungsten
5” (127)
10-15K
PTM23CC001NON
Pt/Ir
2” (51)
10K
PTM3CC02INS
Pt/Ir NS fine
3” (76)
200K
*All have a stainless steel outer shaft
Probe Outer
Diameter (total)
0.013” uninsulated (325 µm)
0.016” insulated (400 µm)
0.018” insulated (450 µm)
0.020” uninsulated (525 µm)
0.013” insulated (325 µm)
Tip
Core diam.
Y dim. X dim. w/ polyimide
Diam.
(pkg of 5)
3-4 µ
.003” (76 µm)
0.4 mm
.005” (127 µm)
3-4 µ
003” (76 µm)
0.4 mm
.005” (127 µm)
3-4 µ
005” (127 µm)
0.4 mm
.008” (203 µm)
3-4 µ
0.01” (254 µm)
0.4 mm
.014” (356 µm)
2-4 µ
0.002” (50.8 µm)
.25 mm
.004” (114 µm)
Selection Guide for Metal Electrodes
ItemLengthInsul.
ShaftNominalTip Diam.Typical Use
Thick.Diam.Impedance
(± 20%)
AMPLIFIERS, ELECTROMETERS
Tungsten — Profile APackage of 10
TM31A10
76 mm
1 µ
0.127 mm
1.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM31A20
76 mm
1 µ
0.127 mm
2.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM31C05
76 mm
1 µ
0.085 mm
0.5 MΩ
1 µRecording from small tightly packed cells
TM33A05
76 mm
3 µ
0.127 mm
0.5 MΩ
1 µMulti unit and single unit recording and microstimulation
TM33A10
76 mm
3 µ
0.127 mm
1.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM33A20
76 mm
3 µ
0.127 mm
2.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM33B01
76 mm
3 µ
0.254 mm
0.1 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33B05
76 mm
3 µ
0.254 mm
0.5 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33B10
76 mm
3 µ
0.254 mm
1.0 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33B20
76 mm
3 µ
0.254 mm
2.0 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33C05
76 mm
1 µ
0.085 mm
0.5 MΩ
1µ
Single unit and stim / chronic use
TM33C10
76 mm
1 µ
0.085 mm
1.0 MΩ
1µ
Single unit and stim / chronic use
Tungsten — Profile CPackage of 10
TM31A10KT
76 mm
1 µ
0.216 mm
1.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM33A10KT
76 mm
3 µ
0.216 mm
1.0 MΩ
1 µMulti unit and single unit recording and microstimulation
TM33B01KT
76 mm
3 µ
0.356 mm
0.1 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33B05KT
76 mm
3 µ
0.356 mm
0.5 MΩ
1-2 µ
Single and multi unit recording and microstimulation
TM33B10KT
76 mm
3 µ
0.356 mm
1.0 MΩ
1-2 µ
Single and multi unit recording and microstimulation
Elgiloy®/Stainless — Profile APackage of 10
SSM33A70
76 mm
3 µ
0.229 mm
7.0 MΩ
1-2 µRecording and Stimulating (Prussian blue staining)
SSM33A120
76 mm
3 µ
0.229 mm
12.0 MΩ
1-2 µRecording and Stimulating (Prussian blue staining)
Elgiloy®/Stainless — Profile CPackage of 10
SSM33A20KT 76 mm
3 µ
0.356 mm
2.0 MΩ
1-2 µRecording and Stimulating (Prussian blue staining)
Tungsten — Profile BPackage of 10
TST33A001KT 76 mm
3 µ
0.356 mm
10 kΩ
1µ
Tissue slice stimulation
TST33A05KT 76 mm
3 µ
0.356 mm
0.5 MΩ
1µ
Stereotrode / Bipolar, differential measurements
TST33A10KT 76 mm
3 µ
0.356 mm
1.0 MΩ
1µ
Stereotrode / Bipolar, differential measurements
TST33A20KT 76 mm
3 µ
0.356 mm
2.0 MΩ
1µ
Stereotrode / Bipolar, differential measurements
TST33C05KT 76 mm
3 µ
0.216 mm
0.5 MΩ
1µ
Stereotrode / Bipolar, differential meas. — extra fine (75 µm separation)
TST53A10KT 127 mm
3 µ
0.356 mm
1.0 MΩ
1-2 µ
Stereotrode / Bipolar, differential measurements
Pure Iridium — Profile APackage of 10
IRM23E10
50 mm
3 µ
0.106 mm
1.0 MΩ
1-2 µ
Single and multiunit recording and stimulation
IRM23E15
50 mm
3 µ
0.106 mm
1.5 MΩ
1-2 µ
Single and multiunit recording and stimulation
IRM23E25
50 mm
3 µ
0.106 mm
2.5 MΩ
1-2 µ
Greater selectivity - small cells
IRM23E30
50 mm
3 µ
0.106 mm
3.0 MΩ
1-2 µ
Greater selectivity - small cells
Pure Iridium —
IRM23E01KT
IRM23E20KT
IRM23E25KT
IRM23E30KT
Profile CPackage of 10
50 mm
3 µ
0.180 mm
0.1 MΩ
2-3 µMultiunit & ERP recording & stimulation
50 mm
3 µ
0.180 mm
2.0 MΩ
1-2 µ
Greater selectivity & microstimulation
50 mm
3 µ
0.180 mm
2.5 MΩ
1-2 µ
Greater selectivity - small cells
50 mm
3 µ
0.180 mm
3.0 MΩ
1-2 µ
Greater selectivity - small cells
Eligoy Steel *Cobalt/chromium/nickel alloy. The KT suffix refers to Kapton™ cladding.
All Metal Microelectrodes are available in custom lengths, blunt or heat treaded (extra charge).
Ordering:
Add the B suffix where blunt electrodes are desired. (For example, an IRM123A10KT ordered as a blunt will be IRM123A10KTB.)
Add the H suffix where heat treated electrodes are desired. (For example, an IRM123A10KT ordered as with heat treatment will be IRM123A10KTH.)
Additional metal
microelectrodes
available on website
www.wpiinc.com
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
89
Measure hydrostatic pressure
in small vessels and oocytes
AMPLIFIERS, ELECTROMETERS
Model 900A Micropressure System
● Simultaneously measures elec­tric potential and pres­sure
● Preset internal mi­cro­elec­trode pressure
● Air-filled system — no de­bub­bling
Model 900A is designed to measure hydrostatic
pres­sure in small vessels and cells. Pressure ranges
of -200 to +400 mm Hg can be measured with
stability and accuracy. The system’s sensing element
is an electrolyte-filled glass microelectrode with a tip
di­am­e­ter range of 2 to 5 microns.
Pressures of electrolyte solutions are measured
by maintaining a salt concentration gradient at the
tip of the sensing electrode in dynamic equilibrium
by applying an equal air pressure inside the mi­cro­
elec­trode. The pressure reading appears on the front
pan­el display and via the BNC recorder output.
Because the piezoelectric pressure controller uses
external pressure and vacuum sources, pressures
low­er than -200 to greater than +400 mm Hg can
be quickly and accurately measured at the mi­cro­
elec­trode tip.
The open pressure chamber is almost immune
to vibrations and movements and, unless they are
ex­treme­ly large, the open system is unaffected by
leaks. The pressure controller is contained in a small,
light­weight enclosure that can easily be mounted
near the micropipette to help reduce dead space.
It in­cludes an amplifier, a piezoelectric valve and
a pres­sure transducer. The user supplies fluid-filled
mi­cro­elec­trodes, +500 mm Hg pressure source and
a -300 mm Hg vacuum source.
Measuring electric potential and pressure si­mul­ta­
neous­ly lets you use potential recording as an ad­di­
tion­al cue for locating the electrode where vis­i­bil­i­ty
is limited, or correlate pressure and potential when
this is meaningful.
The unique “Set Pressure” mode lets you preset
the internal pressure of the microelectrode — select
90
a positive pressure for flushing the tip, or a negative
pressure for pulling solution into the tip. By dis­con­
nect­ing the microelectrode holder and attaching the
tubing to a manometer, you can check the cal­i­bra­
tion against a standard.
A built-in alarm sounds to indicate maximum
pres­sure. The alarm also sounds when the tip is
blocked or electrical continuity is bro­ken (e.g., the
mi­cro­elec­trode comes out of the so­lu­tion, too little
filling so­lu­tion to cover the Ag/AgCl pellet, dis­con­
nect­ed ground reference, etc.).
The piezoelectric pres­sure controller regulates
in­ter­nal pipette pressure by con­trol­ling air flow into
and out of a small pressure cham­ber. A vacuum
source is con­nect­ed on the outlet side of the cham­
ber, and a pi­ezo­elec­tric valve meters air en­ter­ing
the pres­sur­ized cham­ber. The re­sid­u­al vol­ume of
the pres­sure cham­ber includes the mi­cropi­pette, the
con­nect­ing tub­ing and the pres­sure trans­duc­er on
the outlet side of the piezo­elec­tric valve. The 900A
ac­cu­rate­ly con­trols and ad­justs the pres­sure in the
cham­ber to match pres­sures ap­plied ex­ter­nal­ly to
the mi­cro­elec­trode tip.
The response time of the piezoelectric valve is
0.5 ms from fully closed to fully open. Overall sys­
tem response time de­pends large­ly on the amount
of residual vol­ume in the tub­ing. When this volume
is small, the system re­sponds very rap­id­ly (typically
10 mil­li­sec­onds).
The lightweight pres­sure controller pod may be
mounted close to the mi­cro­elec­trode using smallbore tubing, to min­i­mize system dead space.
Microelectrode holders MEH6RF and MEH6SF
for 1.0 mm O.D. capillary glass included. (1.2, 1.5
and 2.0 mm also available — please specify when
or­dering.)
SYS-900A
Micropressure System
System price includes a one-day technical training session at WPI in Sarasota, Florida.
Specify line voltage
OPTIONAL ACCESSORIES
900AP
Replacement Probe
CAL900A
Pressure Calibration Chamber
3491
Probe Extension Cable
2933
Rack Mount Kit
5332
Replacement Liquid Trap
MEH6RF
Micropipette Holder (1.0, 1.2, 1.5 or 2.0 mm — Specify O.D.)
MEH6SF
Micropipette Holder (1.0, 1.2, 1.5 or 2.0 mm — Specify O.D.)
TIPTW900A Prepulled Micropipette for 900A (1 mm thin-wall, 2 µTip) (pkg of 10)
900APP
Replacement Pressure Pod
SYS-PM015DPressure Manometer (15 psi)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
4-Channel Transducer Amplifier
FORT100
PNEU
FORT100
Transducers not included. See WPI’s complete line of transducers for
physiological measurement.
TRANSBRIDGE SPECIFICATIONS
Channels 4
Voltage amplification
x1, x10, x100, x1000
Voltage offset adjustment
> ± 50 mV
Noise
.4uV p-p (0.1 to 10Hz, G=100)
Linearity
+/-.001% of FSR G=1; +/-.01% of FSR, G-=1000
Output voltage swing
± 10 V
Maximum output current
2 mA
Input impedance, each input1010 ohms
TRANSDUCERS
PNEU01Pressure Sensor, 1 psig
PNEU05Pressure Sensor, 5 psig
PNEU15Pressure Sensor, 15 psig
LUMEPhotocell
FORT10-100Force Transducer, Dual Range (10 g and 100 g)
FORT10g
Force Transducer (10 g)
FORT25
Force Transducer (25 g)
FORT100 Force Transducer (100 g)
FORT250 Force Transducer (250 g)
FORT1000 Force Transducer (1000 g)
FORT5000 Force Transducer (5000 g)
3491Probe Extension Cable
Caution: Extension cable may diminish signal-to-noise ratio.
Transducer Excitation
10 V DC (±5 V) approx.
Bandwidth, small signal
1 MHz (x1), 80 KHz (x10), 10 KHz (x100), 1.0 KHz (x1000)
Dimensions
8.5 x 5.12 x 10 in. (21.6 x 13 x 25.44 cm)
Shipping weight
11 lb (5 kg)
SYS-TBM4MTransbridge Transducer Amplifier
Specify line voltage
OPTIONAL ACCESSORIES
13024
Single Rack Mount Kit
13025
Dual Rack Mount Kit
500184 BNC-to-BNC cable, 10 ft
3161
8-pin DIN plug
3718Package of 4, 8-pin DIN (startup kit)
See Transducers, pages 86-87
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
91
AMPLIFIERS, ELECTROMETERS
Transbridge (TBM4M) is a
four-channel analog transducer
manifold, specifically designed
to amplify output voltage
signals from pressure, force,
displacement, and temperature
transducers as well as a wide
variety of other signal sources.
Analog output signals are
available from each channel
for input to a data acquisition
system for digital signal
processing in a computer.
Each channel contains a
regulated 10-volt power supply
(+5 and -5 volts with respect
to signal ground) to provide
DC power to transducers, and
a precision differential amplifier with selectable voltage amplification and
variable position adjustment control.
Transducers can be connected to Transbridge via any of the 8-pin
connectors on the front panel. Four spare 8-pin DIN plugs are
provided with each instrument to allow you to rewire cables of other
manufacturers’ transducers and connect them to Transbridge. Each
Transbridge channel may be used in either Full Bridge or the Half Bridge
mode independently. For transducer types other than resistive bridges,
such as active transistor circuits, magnetic, photocell or piezoelectric
devices, the instrument’s differential amplifiers may still be used
effectively for signal amplification in differential (full bridge) and singleended (half bridge) modes. See WPI’s complete line of TRANSDUCERS for
physiological measurements.
Feature
Input
Output
Biphasic,
Digital Analog
Modeling
Digital from
DS8000
0-100 V
1 μA to 10 mA
SIU/built-in
stimulator
TTL
0-100 V
1 μA to 10 mA
Mono/Biphasic
TTL
Compatible Stimulator:
DLS100
0
00
8
DS
A320
0
00
8
DS
00
A3
10
A3
STIMULATORS, ISOLATORS
A365
±100 V
1 μA to 10 mA
0
00
8
DS
00
A3
10
A3
A385
High Current
TTL
±36 V
10 μA to 100 mA
0
00
8
DS
00
A3
10
A3
A395
Analog
Analog
±10 volts
±70 V
1 μA to 10 mA
Analog
0-1 volt
±500 nA
0
00
8
DS
00
A3
10
A3
DUO 773
Intracellular
Amplifier
92
0
00
8
DS
00
A3
10
A3
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
A310
Accupulser™
Optional footswitch #3259
A310 ACCUPULSER™ SPECIFICATIONS
TIMING PARAMETERS
EVENT INTERVAL
100 µs to1000 s*
A pulse generator/stimulator combining the reproducibility and accuracy
of digital electronics with the fine resolution and continuous adjustment
pos­si­ble with analog circuitry. All timing pa­ram­e­ters are entered with tenturn readable po­ten­ti­om­e­ters and six-position range switches. Outputs are
ac­cu­rate to within 1% of the set value.
Pulses can be created in continuous run, single-shot, or train/burst
modes. Duration of the train/burst is easily controlled using the onboard
envelope generator or by using either of two external gating inputs. Used
in con­junc­tion with the A360, A365, A385, or A395, bipolar pulses or
trains may be easily produced. Output stimulus can be fed through the
Duo 773 for iontophoresis. Footswitch allows hands-free operation.
Three separate outputs are available on the front panel. A Monitor
output provides 10-15 V signals (up to 50 mA) for viewing the output on
an os­cil­lo­scope or for controlling other devices. The stimulator’s signal,
si­mul­ta­neous­ly available at the Isolator output, is sufficient to drive any
WPI A300 Series stimulus isolator (A360, A365, or A385) and is also TTL
and CMOS compatible. The Variable output can provide signals varying
between ±10 V with a resolution of 1 mV. Separate variable outputs are
provided for positive and negative signals.
EVENT DELAY 10 µs to 100 s *
PULSE WIDTH
10 µs to 100 s *
TRAIN DURATION (ENVELOPE)
100 µs to 1000 s*
PULSE INTERVAL
20 µs to 100 s*
EXTERNAL SYNC
Accepts 1-µs min­i­mum pulses TTL, CMOS compatible
SYS-A310 Accupulser™ Signal Generator
Specify line voltage
OPTIONAL ACCESSORIES
3259
Footswitch for A310
2933
Rack Mount Kit, 51⁄4 in. high
EXTERNAL GATE
Accepts 1-µs pulse to continuous
TTL, CMOS compatible
POWER
95-130 V or 190-260 V, switch selectable single phase, 50/60 Hz
DIMENSIONS
17 x 5.25 x 10 in. (43 x 13 x 25 cm)
SHIPPING WEIGHT
14 lb (6.4 kg)
OUTPUTS
SYNC 5 µs, TTL, and 5 V CMOS com­pat­i­ble, 20 mA max.
MONITOR
10-15 V, 50 mA max.
ISOLATOR TTL & 5 V CMOS com­pat­i­ble, 20mA max.
VARIABLE (Pos or Neg)
PULSED/DC
LOW RANGE
HIGH RANGE
Range
0 to ±1 V 0 to ±10 V
Resolution
1 mV
10 mV
NOISE
Pulsed at 100 kHz bandwidth <500 µV
DC Wide Band
<500 µV
OUTPUT IMPEDANCE
<1 Ω
INPUTS
*Continuously variable in six ranges. All accuracies better than 1% of set value.
50kHz maximum pulse frequency.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
93
STIMULATORS, ISOLATORS
Combining the accuracy of dig­i­tal
electronics with the con­ve­nience of
analog controls
STIMULATORS, ISOLATORS
A300 Pulsemaster™
Multi-Channel Stimulator
An integrated five-channel pulse
generator/stim­u­la­tor including
one in­ter­val generator, five pulse
or train channels, two mixer chan­
nels and one very quiet vari­able
voltage out­put stage
The Pulsemaster™ (Model A300) is
WPI’s third generation, multichannel,
pulse/train gen­er­a­tor/stimulator that
combines the superb accuracy of dig­i­tal
elec­tron­ics with the “you-see-what-youget” displays only available on singlechannel products. In one compact rack
mountable enclosure, the Pulsemaster
contains an event interval generator,
five pulse train channels, two mixing
channels and a very quiet variable
voltage output channel. Sys­tem timing
is accurate to 100 ppm; output timing
is continuously variable in 0.1% of full
scale in­cre­ments over a range of eight
orders of magnitude. Bright, threedigit LED displays continuously and
simultaneously show all the variable
timing pa­ram­e­ters.
The Pulsemaster is designed for ease
of use and flex­i­bil­i­ty. Each channel can be operated synchronized
with the onboard event interval generator, trig­gered manually
from any other channel or external source, and as an in­de­pen­dent
asynchronous pulse generator. Except for the external source, all
channel in­ter­con­nec­tions are accomplished on the panel, without
the use of cables. The output from each channel is compatible with
standard digital circuitry and is also designed to drive WPI’s A300
series stimulus iso­la­tors. If desired, any channel’s output may be
in­ter­nal­ly connected to the variable channel, whose am­pli­tude can be
continuously adjusted from mil­li­volts to ten volts.
SYS-A300Pulsemaster™ Multi-Channel Stimulator
Specify line voltage
A300 PULSEMASTER SPECIFICATIONS
EVENT INTERVAL CHANNEL
MIXER CHANNEL (2 provided)
Operating Modes
Inputs
Any combination of an EXTernal pulse, the outputs of the five Pulse Train channels, and DC continuous ON/
DC MOMentary EXT INPUT accepts ≥ 1µs pulses
Output
OUTPUT, TTL, 5V CMOS compatible, 4 mA sink and source
EXTernal SYNC, SINGLE EVENT, CON­TIN­U­OUS ON
Input
EXT SYNC accepts ≥ 1µs pulses; TTL, CMOS, RS232C compatible
Timing
EVENT INTERVAL 10 µs to 999 s (100 kHz - 0.001 Hz), ±0.1% of full scale, continuously variable in 0.1% of full scale increments, through three orders of magnitude, in six ranges
Output
SYNC OUT pulse of ≈6 µs, TTL, 5 V CMOS compatible
PULSE TRAIN CHANNEL (5 provided)
VARIABLE CHANNEL
Inputs
Output from any one PULSE TRAIN channel or one of the two MIXER channels or DC
Output
0 to +1 V low range, 1 mV resolution
0 to +10 V high range, 10 mV resolution
5 mA max sink and source
Operating Modes
EXTernal SYNC, SELF SYNC, manual SINGLE event, sync from Event Interval, sync from any of other four Pulse Trains, sync from one of the MIXers, off, TRAIN/PULSE
Input
EXT SYNC accepts ≥ 1µs pulses; TTL, CMOS
Noise
<500 µV peak @ 100 kHz bandwidth, PULSED mode
<500 µV, wide band, DC mode
Timing
DELAY and WIDTH 10 µs to 999 s, ±0.1% of full scale
Signal Ground
Floating, i.e., not connected to chassis
Output
OUTPUT PULSE/TRAIN of preset timing, TTL, 5 V CMOS compatible, 4 mA sink and source
POWER
95-135 V or 220-240 V, 50/60 Hz
BATTERIES
Three 1.2 V DC, size AA, NiMH batteries
DIMENSIONS
8.5 x 19 x 8.75 in. (22 x 45 x 22 cm)
Output Impedance <1 ohm
SHIPPING WEIGHT 21 lb (9.5 kg)
94
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Isostim™ Stimulator/Isolator
Combining the ease of use and accuracy of WPI’s 300 Series stim­u­la­tors
with the power output of a stimulus isolator
Timing
Pulse interval
and width are set
with single-turn
continuously variable
con­trols from 5 ms to 5.5 s in three ranges. Pulse width is
continuously variable from 50 µs to 550 ms in four ranges.
Current delivery
Stimulus currents up to 10 mA can be set on the front panel with a control
knob and a two-po­si­tion range switch. Output current is load-in­de­pen­dent.
Modes of operation
Dual tone audible alarm
Power
Isostim model A320D is powered by readily ob­tain­able 9-volt alkaline batteries
(in­clud­ed). Un­der average use these will last several months before re­place­
ment is required. The re­charge­able A320R is supplied with a nickel metal
hydride battery stack which provides 10-12 hours of operation before recharge
is required. The A362 Battery Charg­er must be used with the A320R.
A tone sounds when an open circuit is detected or when system compliance
is reached. A second tone, which sounds when a signal is applied to the input,
can only be heard if the batteries have sufficient charge to operate the isolator.
A violation light advises when pulse width exceeds the interval.
ISOSTIM™ SPECIFICATIONS
TIMING PARAMETERS
Interval
5 ms to 5.5 s continuously variable in three ranges (0.18 to 200 Hz)
Pulse width
50 µs to 550 ms continuously variable in four ranges
INPUT
External sync
Accepts 1 µs minimum pulses
External gate Accepts 1 µs pulse to continuous
External command voltage
5.0 V at 3.0 mA (TTL level), 10 V max.
Trigger threshold
2.0 V at 0.5 mA
OUTPUT
Waveform
DC, pulse from internal timing or externally generated pulse
Current ranges
0-1 mA, 0-10 mA
Load voltage excursion (com­pli­ance) 100 V nom., 150 V max.
Output polarity Reversible, manual switch
Current rise time and delay
8 µs, typical (1 K Ω load)
Current fall time and delay
10 µs, typical (1 K Ω load)
Leakage resistance, output to ground 1012 Ohms
Optocoupler
2500 V rated min. breakdown voltage
POWER
Dry Cell (Version D)
16 alkaline 9V batteries included
Rechargeable (Version R)
16 rechargeable NiMH 9V batteries incl
DIMENSIONS
8.5 x 3.5 x 4.9 in (22 x 9 x 12 cm)
SHIPPING WEIGHT
4 lb (1.8 kg)
A362 Battery Charger
Required for A320R, A365R and A395R
Recharges the high-voltage nick­el-cad­mi­um or NiMH battery stack in the
A320R, A365R or A395R. LED lamp indicates charg­ing sta­tus. Full charge
over­night. Di­men­sions: 2.8 x 4.1 x 5 in. (7 x 10 x 13 cm). Ship­ping
weight: 4 lb (1.8 kg).
SYS-A362
A320RC
SYS-A320D SYS-A320R
Battery Charger for A320R, A365R, A395R
A320R with Charger (A362)
Isostim™ Stimulator/Isolator
Isostim™ Stimulator/Isolator (rechargeable)
Specify line voltage
OPTIONAL ACCESSORIES
DRL
Dummy Load Resistor Kit (set of 3)
13347
BNC-to-Double Banana Adapter
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
95
STIMULATORS, ISOLATORS
In FREE RUN, Isostim™ generates con­tin­u­ous square waves. In EXT GATE or
EXT SYNC modes, externally applied pulses can generate trains or single
events. Single pulses of finite duration can be produced using a push-button
on the instrument’s front panel. EXT/DC mode converts Isostim to a passive
stimulus isolator.
STIMULATORS, ISOLATORS
Stimulus Isolator / Precision Current Source
Model A365 includes the same features and specifications as A360
but with the added capability for automated bipolar pulsing for zero net
charge on biological preparations.
Polarity — Output polarity is determined by a push switch on the front
panel. Bipolar current is toggled by the command waveform, setting al­ter­
nat­ing pulses as positive or negative.
SYS-A365D
A365RC
SYS-A365R
SYS-A362
A365 SPECIFICATIONS
OUTPUT WAVEFORM
DC or current pulse
OUTPUT CURRENT RANGES
0.1, 1.0, and 10 mA
CURRENT AMPLITUDE ERROR
0.5% of full scale, max.
CURRENT RESOLUTION
0.1% of full scale, typical
OUTPUT LOAD VOLTAGE
EXCURSION (COMPLIANCE)
100 V
EXTERNAL COMMAND VOLTAGE
5.0 V at 3.0 mA (TTL level), 10 V max.
TRIGGER THRESHOLD
2.0 V at 0.5 mA
OUTPUT POLARITY
Reversible, manual switch or automatic
CURRENT RISE TIME & DELAY
6 µs, typical (1 KΩ load)
CURRENT FALL TIME & DELAY
10 µs, typical (1 KΩ load)
OUTPUT TO GROUND RESISTANCE
1012Ω
OPTOCOUPLER
2500 V, rated min. breakdown voltage
POWER
Model A365D (dry cell)
Model A365R (rechargeable)
16 alkaline 9 V batteries, included
16 rechargeable NiMH 9 V batteries incl.
DIMENSIONS
8.5 x 3.5 x 5 in (22 x 9 x 12 cm)
SHIPPING WEIGHT
4 lb (1.8 kg)
96
High Voltage Isolator, Bipolar, alkaline batteries
A365R with charger (A362)
High Voltage Isolator, Bipolar, rechargeable
Battery Charger for A320R, A365R, A395R
Specify line voltage
OPTIONAL ACCESSORIES
DRL
Dummy Load Resistor Kit (set of 3)
3468
Dual Rack Mount Kit for A365
3469
Single Rack Mount Kit for A365
13347
BNC-to-Double Banana Adapter
DRL —
Dummy Load
Resistor Kit
Converts current
output to precise
voltages.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Combines optical isolation with a ±100 mA current generator
A385 High
Current Stimulus
Isolator
A385RC A385R with A382 Charger
SYS-A385R
High Current Isolator, rechargeable
SYS-A382
Battery Charger for A385 (see below)
Specify line voltage
OPTIONAL ACCESSORIES
3468
Dual Rack Mount Kit
A385 SPECIFICATIONS
OUTPUT WAVEFORM
DC or current pulse
OUTPUT CURRENT RANGES
1, 10, and 100 mA
CURRENT AMPLITUDE ERROR
0.5% of full scale, max
A382
Battery
Charger
CURRENT RESOLUTION
REPEATABILITY
0.1% of full scale, typical
OUTPUT LOAD VOLTAGE
EXCURSION (COMPLIANCE)
36 V
An innovative threestep charg­er, A382
employs fast, medium, and
trickle charg­es at a safe, low cur­rent, great­ly ex­tend­ing bat­tery life. After
a fast ini­tial phase, the charg­er au­to­mat­i­cal­ly switches to a constant
voltage mode. When charg­ing is com­plete, the charg­er switch­es to the
trickle-charge mode. LED lamps indicate charging status. (For use only in
charging batteries in­stalled in the A385.)
EXTERNAL COMMAND VOLTAGE
5.0 V at 3.0 mA (TTL level), 10 V max.
TRIGGER THRESHOLD
2.0 V at 0.5 mA
OUTPUT POLARITY
Reversible, manual switch, or electronically switched bipolar delivery
CURRENT RISE TIME AND DELAY
6 µs, typical (1 KΩ load)
CURRENT FALL TIME AND DELAY
10 µs, typical (1 KΩ load)
OUTPUT TO GROUND RESISTANCE
1012 Ω
OPTOCOUPLER
2500 V, rated minimum breakdown voltage
POWER
Six rechargeable lead-acid batteries (Requires companion charger A382)
POWER
95-135 V or 220-240 V, 50/60 Hz
DIMENSIONS
8.5 x 3.5 x 5 in. (22 x 9 x 12 cm)
DIMENSIONS
8.5 x 3.5 x 5 in (22 x 9 x 12 cm)
SHIPPING WEIGHT
5 lb (2.3 kg)
SHIPPING WEIGHT
5 lb (2.3 kg)
A382 SPECIFICATIONS
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
97
STIMULATORS, ISOLATORS
Delivers positive, negative, or bipolar currents. For bipolar delivery,
polarity of the output is toggled to the opposite state with each pulse
presented to the input. Pulse duration is controlled by an externally
applied voltage. In­put connector is a standard BNC, allowing signals from
any source — such as computer D/A or I/O lines — to be used.
Output amplitude is set on a 3-digit, ten-turn dial as a percentage of
the range selected: for example, a setting of 45.6 in the 0-10 mA range
trans­lates to 4.56 mA at the output. Ac­cu­ra­cy and repeat­abil­i­ty are ex­cel­
lent. Designed for sub­cu­ta­ne­ous stim­u­la­tion, max­i­mum out­put volt­age at
the stim­u­lat­ing elec­trodes is 36 volts, re­duc­ing the pos­si­bil­i­ty of se­ri­ous
ac­ci­den­tal tran­s­cu­ta­ne­ous shocks. A com­pli­ance/out­put alarm sounds
when the 36-volt lim­it is reached. In­ter­nal cir­cuit­ry main­tains elec­trodes
short-cir­cuit­ed dur­ing in­ac­tive pe­ri­ods (“elec­trode ex­haust­er” fea­ture).
A385 is not ap­pro­pri­ate for tran­s­cu­ta­ne­ous stim­u­la­tion.
The 1.2 amp-hour rat­ing of the six heavy-duty lead-acid re­charge­able
batteries en­sures that ex­per­i­ments will not be in­ter­rupt­ed by dead bat­ter­
ies — even at peak currents. Indicator lights and audible alarms keep the
user constantly apprised of battery charge status. These batteries must be
recharged by the A382 Sys­tem Charger designed especially for the A385.
A395 Linear Stimulus Isolator
STIMULATORS, ISOLATORS
Replicates a programmed waveform of any shape or polarity
All WPI stimulus isolators are designed to supply constant current because
current threshold (not voltage) is the most quantitatively reproducible
parameter for stimulation of nerve and muscle. Model A395 dispenses
current reproducibly from its Output terminals; the amplitude being
determined by the selected current RANGE and the input voltage. Current
amplitude is “constant”, that is, load resistance independent, provided
that the I x R (load) product does not exceed the available battery supply
voltage. A visual indicator (the compliance LEDs) displays if I x R reaches
this limit. When the unit is out of compliance, one of the two LEDs
(labeled - and +) illuminate, depending in which direction the current is
flowing. Model A395 D can generate a voltage of 70 volts or more across
its OUTPUT terminals. Thus, the user can be sure that the amplitude of
the current will be as dialed as long as the voltage drop across the load
(stimulus electrode path) does not reach the magnitude of the supply
voltage. The compliance LEDs will then be visible. The user would then
know that (a) too much current was dialed for a given load or (b) interelectrode resistance was too high or the electrode circuit path was open.
Model A395 generates an output current of arbitrary (user-defined)
wave shape; DC, AC, pulse, and combinations thereof. Battery operated,
and pho­to­elec­tri­cal­ly-isolated from the input voltage drive, the instrument
regen­er­ates out­put currents which are linearly proportional to the analog
voltage wave­forms pro­vid­ed by your D/A con­vert­er or sig­nal generator
(see diagram below).
The A395 is ideally suited for data acquisition and stimulator
generators. It can be easily daisy-chained for mutiple channel
requirements.
Current Delivery —A 10 V input
produces the maximum output
current for the current range
selected, i.e., 100 µA, 1 mA,
or 10 mA. Front panel controls
allow DC current to be generated.
Externally applied signals can be
superimposed si­mul­ta­neous­ly (DC
offset). Warning lamps indicate
open circuit or ex­ces­sive current
conditions.
Digital Meter — Measures DC or average output current.
Overload Lamps — Indicate when output voltage has reached positive or
negative compliance voltage limit.
A395RC SYS-A395D
SYS-A395R
SYS-A362
A395R with Charger (A362)
Linear Stimulus Isolator
Linear Stimulus Isolator, Rechargeable
Battery Charger
Specify line voltage
OPTIONAL ACCESSORIES
3468
Dual Rack Mount Kit
3469
Single Rack Mount Kit
A395 SPECIFICATIONS
10 V
INPUT
3 ranges: 100µA, 1 mA, and 10 mA
OUTPUT VOLTAGE RANGE
± 70 V
OUTPUT BANDWIDTH
10 kHz (measured across 1KΩ load R)
INPUT RESISTANCE
> 20 MΩ
INPUT VOLTAGE @ Imax
± 10 volts
INPUT/OUTPUT LINEARITY ERROR
< 0.5%
RISE, FALL TIME
26 µs @ 10 KΩ
POWER
Model A395D Model A395R
17 alkaline 9 V batteries
17 rechargeable NiMH 9 V batteries
DIMENSIONS
6.5 x 4 x 3.5 in. (16 x 10 x 9 cm)
SHIPPING WEIGHT
4 lb (1.8 kg)
0V
OUTPUT
-10 V
A362 Battery Charger
+100 µA, +1 mA, +10 mA
Required for A320R, A365R and A395R
0
-100 µA, -1 mA, -10 mA
pts
Acce g
o
anal t
inpu
98
OUTPUT CURRENT, Imax
Recharges the high-voltage nickel-cad­mi­um or NiMH
battery stack in the A320R, A365R or A395R. LED
lamp in­di­cates charg­ing status.
Full charge over­night.
Di­men­sions: 2.8 x 4.1 x 5 in.
(7 x 10.5 x 12.7 cm).
Ship­ping weight: 4 lb (1.8 kg).
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
DS8000 8-Channel Digital Stimulator
● Single board computer w/ LCD touch screen
● 32 separate outputs ( 24 BNC's front panel )
● Scope mode displays waveforms
● Unipolar, bipolar, paired pulse, sine, ramp, custom
● 8 banks of 3 timers, synchronous / asynchronous
● Custom upload of real biopotentials
● 8 internal and 8 external trigger inputs
● 2 independent full parameter memory banks
DS8000
8-Channel Digital Stimulator
Output Noise
Timing Accuracy
OUTPUT Voltage Resolution
Max. output voltage
Output impedance
External TRIGGER sync
Digital I/O
Mains voltage
Dimensions
SHIPPING Weight
Ambient temperature
Humidity
< 5 mV rms
< 100 ppm
5 mV
+/-10V @ +/- 10 mA @ 0.005 V/step
50 Ohm Analog, < 1 ohm Combined Analog
40 µs minimum pulse TTL, CMOS
20 µs glitch and spike protection
5V max 10 mA (input)
85-260 V AC, 45-65 Hz 50W
13.3 cm x 42.5 cm x 25.4 cm
5.25” x 16.73” (19” rack) x 10”
12 lb (5.5 kg)
-10 to +40 °C; -20 to +50 °C (Internal)
Max. 95% relative humidity, non-condensing
SPECIFICATIONS SUBJECT TO CHANGE WITHOUT NOTICE.
Digital Linear Stimulus Isolator
The DLS100 is preferentially optimized for applications in which the DS8000 digital
stimulator is used. DLS100 connects to the DS8000 via a flexible cable through
which it receives power and stimulus signals in a digital format. Up to eight DLS100
isolators can be connected independently to one DS8000.
DLS100
501670
83016
503301
Digital Linear Stimulus Isolator
Adapter, Dual Mini-Banana-to-BNC(F)
Replacement Cable, DLS100-to-DS8000
Cable, mini-DIN extension, 10-ft
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
99
STIMULATORS, ISOLATORS
DS8000 Specifications
Timing parameters
Period (total signal width)
0.04 ms to 10,737,418.24 ms
0.02 ms to 10,737,418.24 ms
Pulse width
Bipolar gap width
0.00 ms to 10,737,418.24 ms
Operating Modes
Free run, triggered, gated, Train, DC
Triggers
8 External, manual, TTL 1-8, combined TTL 1-8, timer start or stop
Train events1-199
Train pulse width
0.02 ms to 10,737,418.24 ms (3 hours)
Train pulse DELAY
0.04 ms to 10,737,418.24 ms
Train period
0.06 ms to 10,737,418.24 ms
BNC Output connectorsAnalog, combined analog, combined digital (TTL)
WaveformsUnipolar, bipolar, rectangular, sine, ramp, step, paired pulse, custom defined
Custom waveform
12 steps/ voltage point (1025 if remote controlled)
VARIABLE step waveform
100 points (1025 if remote controlled)
Part No.
Description
Curing time
Epoxies
Form strong bonding. Used in wire bonding applications.
4898
Silver filled conductive Epoxy
12 [email protected]; 5 min @150C
Connecting conductors that can't be soldered. Constructing or connecting Ag/AgCl pellets.
7335
Carbon filled conductive Epoxy
48 [email protected]; 5 min @150C
Constructing carbon electrode.
4886
High performance Structural Epoxy
12 [email protected]
Forms a strong and slightly flexible bond on plastic, metal, and glass. Bonds some low surface .
Hot melt (EVA)
Easy to use for bonding, needs large gap filling
13316
Mini Glue Gun with glue sticks
As soon as it cools down
LAB SUPPLIES
Silicone Adhesives/Sealants/Primers
Useful Applications and Characteristics
Bonds wood, glass, metals, and many plastics.
Good moisture resistant and elastic. Low toxic.
1571
Room temperature vulcanizing (RTV)
adhesive. Acyloxy/moisture cure system.
Acetic acid is cure by-product.
[email protected]
Has the best adhesion property in this silicone family. Will bond to many materials.
7128
RTV sealant. Alkoxy/Moisture cure
system. Methanol as cure by-product.
[email protected]
Good for bonding or sealing electronics circuits (metal).
SYLG184
Sylgard, Two parts, vinyl/platinum cure
sealant. Hydrogen as cure by-products.
Very low toxic
[email protected], 15 [email protected]
Coating Patch Clamp electrodes, Cell culture dish, making dissection pads.
Kwik-Sil
Two part, adhesive. Vinyl/platinum
system, Hydrogen as cure by-products.
Very low toxic.
< 5 [email protected]
Live tissue and nerve studies. Medium strength adhesion.
Kwik-Cast
Two part sealant. Vinyl/platinum cure
system. Hydrogen as cure by-products.
Very low toxic.
< 5 [email protected]
Sealant for live tissues. Embedding peripheral nerves with electrodes.
6820
Primer for silicone
N/A
Enhances adhesion of silicone adhesives for difficult to bond plastic surfaces
Cyanoacrylate
Forms an instantaneous bonding.
7341
Ethyl Cyanoacrylate, low viscosity
90-120 cps
<10 seconds
Mounting rat/mouse brain slices. Ideal for relatively small gaps and smooth surfaces.Bonds plastic, metals
and rubber. Package of 10 vials, each approximately 1.5 mL.
7342
Ethyl Cyanoacrylate, high viscosity
1100-1600 cps
<30 seconds
Use on brain slice exp. Ideal for larger gaps, allows slightly longer bonding time. Bonds plastic, metals and
rubber. Package of 10 vials, each approximately 1.5 mL.
Vetbond
Butyl Cyanoacrylate, Low toxic
<10 seconds
Bonds tissues, alternative to suture, helps small wound healing. Antimicrobial effect. Used in forensic
science.
503763
Octyl Cyanoacrylate, Low toxic
<15 seconds
Suitable for surface wound bonding, protection, holding a sensor or other device on the tissue.
Scotch-Weld 2216
Structural Epoxy
Kwik-Gard
™
Kwik-Gard is specially packaged
Sylgard 184 silicone for quicker
and easier application, eliminating
the messy procedure of preparing
the mixture before application. Its
special cartridge controls the precise
mixing ratio to ensure proper curing.
The disposable tip mixes resin and
hardener as they are dispensed. Since no air is
introduced during mixing, the resin does not need
degassing for most applications. The mixed silicone
is applied directly to the site, reducing preparation
time and material waste.
Each Kwik-Gard cartridge contains 37 mL of
resin and hardener. The dispensing tip has a dead
volume of 0.75 mL.
100
KWIKGARD Kwik-Gard Start-up Kit (incl. dispenser, 1 cartridge, 5 tips)
KWIKGLUE Kwik-Gard Refill (2 cartridges, 10 dispensing tips)
KWIKMIX Dispensing Tips (pkg of 10)
KWIKGUN Kwik-Gard Dispenser
Probably still the best epoxy for bonding plastic,
often used as the benchmark for testing the
binding strength of other adhesives. The slightly
rubbery texture also makes it less easy to break
off. It is the only epoxy known that can bond PEEK.
Color: gray. Cures at room tem­per­a­ture.
Shipping weight: 1 lb. (0.5 kg)
4886
Scotch-Weld 2216 (2 oz.)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Electrically Conductive
Silver Epoxy
Two-component silver-filled epoxy for elec­tri­cal
connections which cannot be soldered, such as Ag/
AgCl pel­lets. This widely used silver-filled epoxy
features low viscosity and smooth flowing char­ac­
ter. Pure sil­ver is dis­persed in both res­in and hard­
en­er. Cures in 15 min­utes at 120 °C. Mix ra­tio 1:1.
May be premixed and fro­zen for later use.
Shipping weight: 1 lb. (0.5 kg)
4898
Silver Epoxy (1 oz.)
Silicone RTV adhesive
(non-acidic)
Because it is non-corrosive, this material is ide­al for
use on metal, for en­cap­su­lat­ing small circuits on
connectors. After cure, will withstand -55° to 200
°C. No mixing required.
Electrically Conductive
Carbon Epoxy
Shipping weight: 1 lb. (0.5 kg)
Two-component carbon-epoxy, curable at room
and elevated temperatures. Ideal for elec­tro­stat­ic
discharge protection and EMI/RFI shield­ing. 1:1 mix
ratio. May be premixed and fro­zen for later use.
RTV Prime Coat
Shipping weight: 1 lb. (0.5 kg)
7335
Carbon Epoxy (2 oz.)
7128
Clear silicone seal­ant pro­vides good bond­ing to
plas­tic. Af­ter cure, will with­stand -55 to 200 °C. No
mix­ing re­quired. A handy, gen­er­al pur­pose lab­o­ra­
to­ry sealant. (Releases acetic acid during curing.)
Shipping weight: 1 lb. (0.5 kg)
1571
RTV Sealant (4.7 oz.)
RTV Coating (3 oz.)
Mini Glue Gun
Enhances adhesion of silicone adhesives to many
difficult-to-bond plastic surfaces.
Shipping weight: 1 lb. (0.5 kg)
6820
Silicone RTV adhesive
RTV Prime Coat, 400 ml (13.5 oz.)
Comes with three sticks of spe­cial for­mu­la hot melt
glue. UL ap­proved. 110V 60Hz only.
Ship­ping weight: 1 lb. (0.5 kg)
13316Mini Glue Gun
Low toxicity 5-minute adhesives for live tissues!
• Specially formulated for WPI
• Extremely low toxicity
• Excellent moisture resistance
• Cures at room temperature
WPI’s silicone elastomers are based on recently developed vinyl
terminated siloxane and platinum complex catalysts. They exhibit
exceptional low toxicity before, during and after curing. The traditional
RTV silicone systems produce either acetic acid or alcohol during
condensation; these compounds are toxic to living cells. In contrast,
the only by-product of condensation from WPI’s elastomers is a small
amount of hydrogen gas, which was shown not to cause any effect on
nerve activity in highly sensitive peripheral nerves. Both elastomers cure
on contact with the tissue. Currently, WPI provides rapid-curing silicone
elastomers in two different formulas:
Kwik-Sil™ is a translucent silicone elastomer adhesive with medium
viscosity. It has good adhesion
10 mixing tips
and mechanical property (tear
strength and elongation). The
included
very short curing time (approx.
1 minute) make it especially
useful for moving preparations.
KWIK-CAST & KWIK-SIL SPECIFICATIONS
Unmixed
Kwik-SilKwik-Cast
Mix Ratio
1:1
1:1
Working Time, typically
2-10 minutes 2-3 minutes
Viscosity, cps
15,000
10,000
After Curing 24 hours:
Tear Strength
90 ppi
44 ppi
Elongation
650%
60%
Durometer (shore A-2)
30
36
Color
translucent
green
Volume Resistivity, Ω/cm1x10151x1015
Shipping Weight
0.5 lb
0.5 lb
References
Sivitz, et al., “Effect of acute and antecedent hypoglycemia on sympathetic
neural activity and catecholamine responsiveness in normal rats.” Diabetes.
50:1119-1125, 2001.
Andrews and Kunze. “Voltage-gated K+ channels in chemoreceptor sensory
neurons of rat petrosal ganglion.” Brain Research, 897:199-203. April 2001.
KWIK-SIL
KWIK-CAST
600009
Kwik-Cast™ is a very low
viscosity silicone elastomer
sealant. It is able to fill small
10 mixing
tips included
KWIK-CAST
KWIK-SIL
Silicone Adhesive Compound (two 5-mL syringes)
Silicone Casting Compound (two 5-mL syringes)
Replacement Mixing Tips (pkg of 10)
PRICE BREAK
1-5 pkg
6-9 pkg
More than 10
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
101
LAB SUPPLIES
The properties of this adhesive make it exceptionally useful for
neuroscience applications, peripheral nerve studies and similar
biomedical applications. These new silicone elastomers also eliminate
the mess and time involved in pre-mixing other commonly used
formulations (such as Wacker SilGel® and Sylgard®). Each silicone
elastomer is packaged in a double barrel syringe and is automatically
mixed when pressed out of the mixer tip provided. It can then be
applied directly to the tissue without further mixing. The curing time
of these silicones is short, reliable and reproducible thus eliminating
potential costly guesswork when using other tissue adhesives.
Furthermore, the curing process does not produce heat, which can
otherwise cause tissue damage. WPI’s silicone elastomers are much less
toxic than dental silicone because they contain no surfactant additives.
spaces around nerves and creates
a continuous mass ensuring
long-term recording stability.
Kwik-Cast is color-coded so mixing
is foolproof. It can be applied and
cured underneath mineral oil. The
slight longer curing time (approx. 3
minutes) makes it more suitable for
stationary preparations and in vitro
tissue studies.
“Super” Adhesives for Life Science Research
Cyanoacrylate adhesives have been on the market since 1958. Most
with the traditional suture,
industrial or household grade cyanoacrylate is made of shorter alkyl
the new super adhesive
chain derivatives such as methyl or ethyl cyanoacrylate (WPI’s #7341
has several advantages.
and #7342). They are very useful for temporarily holding tissues such
On average, it takes only
as mounting specimens for microtome sectioning. However, they are not
one-tenth of the time
suitable for bonding wounds on live animals. The difficulties of using
to close an incision. The
cyanoacrylate for bonding live animals are: (1) a strong, irritating odor;
bonding strength is equal
(2) quick loss of bonding strength due to breakdown of the bonding by
to 5-0 monofilament suture.
hydration; (3) the breakdown products, cyanoacetate and formaldehyde,
It also has a mysterious
are toxic and can cause inflammatory reactions; and (4) they have low
antimicrobial effect that
flexibility and tend to be brittle.
can decrease infection rates
in contaminated wounds.
To overcome these problems, several longer alkyl chain cyanoacrylates
Bonding will slough off
have been developed especially for veterinary and human use. The first
naturally in 5 to 7 days.
longer alkyl chain product
Cosmetic appearance of the
is butyl cyanoacrylate. This
healed incision is also better.
product has been used
for animal and human
Gluture Topical Tissue Adhesive #503763 forms a strong and flexible
applications outside the USA
film and is thus more suitable for surface wound bonding, protection, and
since 1970. It is much less
holding a sensor or other device on the tissue. Setting time is about 10
toxic and has a lower odor
seconds, which gives ample time for application. It can also be used for
than the methyl or ethyl
temporarily holding a live tissue. For example, there is a report of using it
cyanoacrylate. The butyl
to hold nematodes on a glass slide for patch-clamp neurons recording.
cyanoacrylate offered by WPI
All of the products offered by WPI are veterinary grade (not suitable for
is Vetbond™.
human application). Though very similar to the grade for human use, they
A family of adhesives
are not sterile and do not have FDA approval.
containing octyl cyanoacrylate, a plasticizer and
503763 Gluture Topical Tissue Adhesive (10 tips), 1.5 mL
stabilizer, was developed In the 1990’s (one of them
7341
Cyanoacrylate Adhesive, low viscosity—90-120 cps (package of 10 vials, each approx 1.5mL)
approved by FDA for human use). When bonding to
7342
Cyanoacrylate Adhesive, high viscosity—1100-1600 cps (pkg of 10 vials, each approx 1.5mL)
tissue, these new adhesives are four times stronger
VETBOND3M Vetbond™ Adhesive (3 mL)
and less toxic than butyl cyanoacrylate. Compared
LAB SUPPLIES
Sylgard
Silicone
Dissecting Pad Kit
A two-part silicone elastomer,
ideal for pot­ting and en­cap­su­lat­
ing applications. Very low di­elec­tric
constant sealing com­pound used in
patch clamp­ing and many other lab
applications. After cure, will with­stand
-55° to 200 °C.
Shipping weight: 2 lb. (1 kg)
SYLG184
Sylgard (1.1 lb)
Lab Coat
Light weight, medium length
— for the lab professional
LABCOAT-XS
LABCOAT-S
LABCOAT-M
LABCOAT-L
LABCOAT-XL
Lab Coat, Extra Small
Lab Coat, Small
Lab Coat, Medium
Lab Coat, Large
Lab Coat, Extra Large
SOLD
OUT!
Kit Includes:
2-Part Sylgard silicone elastomer
20 plastic petri dishes with lids, 65mm
Pins
501986
Quantity discount available
102
Make your own silicone dissecting pads easily and quickly. Mix the 2-part
silicone right in the plastic petri dishes and allow to cure 24 hours at room
temperature. Kit includes enough silicone to prepare 20 dishes.
Silicone Dissecting Pad Kit
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Digital Caliper & Micrometer
The high quality electronic digital
caliper and micrometer are useful
tools — no laboratory should be
without one because they are more
accurate and easier to use than the
traditional analog units. Measure
in either inches or millimeters at
the touch of a button. The floating
zero feature allows you to read
the increment without calculation.
Both of them have the SPC output
501601
503036
502157
for interface with external readout
devices. The caliper is made from
hardened stainless. The micrometer
meets the IP54 as defined in the
IEC529 standard that is resistance
to dust and splashing water. The
caliper measures up to 150 mm
(6 in.) with 0.01 mm (or 0.0005")
resolution. The micrometer
measures up to 25 mm (1 in.) with
0.001 mm (0.00005 in.) resolution.
The micrometer is designed to be
water-resistant to IP54 as defined
in the IEC529 standard. The IP54
code’s first digit “5” means dustprotected. The second digit “4”
means that the caliper is protected
against splashing water — it has
to withstand a shower from every
direction for 10 minutes.
Digital Caliper Digital Micrometer
Replacement Battery (package of 10)
Digital
Caliper
Digital Micrometer
Screwdriver Set
Wire Cutters
12cm long
Notched blade
Roger Wirecutting Scissors
LAB SUPPLIES
501321
This production grade precision 5-piece screwdriver set is the highest
quality tool you can find on the market. Made by German craftsmen,
the chrome-vanadium tips will fit any screw securely without leaving
marks. The set contains an ESD safe handle with 8 interchangeable
blades. Phillips Sizes: 000, 00, 0, 1. Slotted Sizes: 1.5, 2.0, 3.0, 3.5
mm. The 000 size Philips blade is the smallest you can find anywhere
— it can fit smallest screw on a 35 mm camera. Weight: 0.24 lb
501635
Magnetic
Heating
Stirrer
Professional Quality Screwdriver Set
The Magnetic Heating Stirrer enjoys the
advantages of convenience, stable and
steady operation as well as indefinite
speed regulation. It can carry out
stable and precise agitation
of solutions in a relatively
common temperature
range. It is especially
suitable for agitation of
small volume samples.
501610 Specifications
Speed Range
Tray Diameter Heating Power Power Supply Dimensions
Stirring Power Temperature
0-1400 rpm
12 cm
100 W
AC 110 V / 50 Hz
23 x 16 x 10 cm
3-6W
20°C - 210°C
WPI-02 Magnetic Heating Stirrer World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
103
g-SPIN™
Microcentrifuge
l Small size with rubber feet keeps the centrifuge stable
l Supplied with interchangeable rotors and adapters for
0.5mL-2.0mL microtubes and PCR strips
l On/off switch lets you start and stop in seconds
l Safety switch stops rotor without cover in place
Also a
v
4,000 ailable:
R PM a
nd
10,00
0 R PM
units
— call
detail for
s.
g-SPIN Specifications
Speed range
6000 rpm, fixed
Max. RCF
2000× g
Tube capacity
6 x 0.5/2.0 mL tubes 2 x 0.2 mL strip tubes
Rotor
Fixed angle 6-place 0.5—2.0-mL tubes 2 strips PCR 0.2 mL tubes
Dimensions
6"W x 5"H x 7"D
Power
110V 60 Hz or 220V 50 Hz
G-SPIN6
G-SPIN6-220
503529
503530
503531
503532
503537
Microcentrifuge, 6000 RPM, 110V 60 Hz
Microcentrifuge, 6000 RPM, 220V 50 Hz, CE
Microcentrifuge tube, 0.5 mL, natural, bag/1,000
Microcentrifuge tube, 1.5 mL, natural, bag/1,000
Microcentrifuge tube, 2.0 mL, natural, bag/1,000
Microcentrifuge tube strips, 0.2 mL, and domed cap strips, bag of 250
Microcentrifuge tube strips, 0.2 mL, and flat cap strips, bag of 250
LAB SUPPLIES
Rackmounting Hardware
Many instruments may be mounted in
standard 19-inch instrument racks with the
ap­pro­pri­ate rackmount kit, as noted on the
page featuring the instrument.
Dual rackmount kits allow many smaller
in­stru­ments to be joined by bolting the
chassis to­geth­er and mounting the pair into
a standard rack.
Brackets for instruments which are less than
17.5 inches wide have “wings” which extend
to the stan­dard rack width.

3484
2932
Rackmount Kit, 3½-in. high (121)
3468
Dual Rackmount Kit
(705, A320, A365, A385, A395)

DAM Series amplifiers and Iso-DAM
amplifiers may be mount­ed to the rack
panels above by fastening bolts (included)
through holes in the panel.


2935
2933


Rackmount Kit for DAM Series amplifiers
Rackmount Kit, 8 3⁄4-in. high
Rackmount Kit, 5¼-in. high (260, 900A, DUO773, A310)
13025 Dual Rackmount Kit (TBM4M)

3469
104
Single Rackmount Kit (705,
A320, A365, A385, A395)

13024 Single Rackmount Kit (TBM4M)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Precious Metal and Specialty Wire
0.
61
!
m
m
Micro Coaxial Cables
Metal
Coating AWG*
Diameter
AGT0510
Silver
Teflon
36
0.005 in. (0.125 mm)1
Precut Length
10 ft (3 m)
AGT0525
Silver
Teflon
36
0.005 in. (0.125 mm)1
25 ft (7.6 m)
AGT05100
Silver
Teflon
36
0.005 in. (0.125 mm)1
100 ft (30 m)
AGT1010
Silver
Teflon
30
0.010 in. (0.25 mm)1
10 ft (3 m)
AGT1025
Silver
Teflon
30
0.010 in. (0.25 mm)1
25 ft (7.6 m)
AGT10100
Silver
Teflon
30
0.010 in. (0.25 mm)1
100 ft (30 m)
AGT1510
Silver
Teflon
26-27
0.015 in. (0.38 mm)1
10 ft (3 m)
AGT1530
Silver
Teflon
26-27
0.015 in. (0.38 mm)1
30 ft (9.1 m)
AGW0510
Silver
—
36
0.005 in. (0.125 mm)
10 ft (3 m)
AGW0530
Silver
—
36
0.005 in. (0.125 mm)
30 ft (9.1 m)
AGW1010
Silver
—
30
0.010 in. (0.25 mm)
10 ft (3 m)
AGW1030
Silver
—
30
0.010 in. (0.25 mm)
30 ft (9.1 m)
AGW1510
Silver
—
26-27
0.015 in. (0.38 mm)
10 ft (3 m)
AGW1530
Silver
—
26-27
0.015 in. (0.38 mm)
30 ft (9.1 m)
AGW2010
Silver
—
24
0.020 in. (0.5 mm)
10 ft (3 m)
AGW2030
Silver
—
24
0.020 in. (0.5 mm)
30 ft (9.1 m)
AGW4010
Silver
—
18
0.040 in. (1.0 mm)
10 ft (3 m)
AUW0170
Gold
—
50
0.001 in. (0.025 mm)
70 ft (21 m)
AUW201
Gold
—
24
0.020 in. (0.5 mm)
1 ft (30 cm)
C3005
Carbon
—
49
0.0012 in. (30 µm)
5 ft (1.5 m)
CPVC4050Y
Carbon (46Ω/ft)
PVC (yellow)
18
0.040 in. (1.0 mm)
50 ft (15.2 m)
CPVC4050G
Carbon (46Ω/ft)
PVC (green)
18
0.040 in. (1.0 mm)
50 ft (15.2 m)
CPVC4050R
Carbon (46Ω/ft)
PVC (red)
18
0.040 in. (1.0 mm)
50 ft (15.2 m)
CPVC4050W
Carbon (46Ω/ft)
PVC (white)
18
0.040 in. (1.0 mm)
50 ft (15.2 m)
CU7T44G
Copper Alloy Nylon/Polyurethane
30
0.010 in. (0.20 mm)
16.5 ft (5 m)
IN1003
Indium
—
30
0.01 in. (0.25 mm)
3 ft (91 cm)
PT1002
Platinum / Iridium
—
30
0.010 in. (0.25 mm)
2 ft (51 cm)
PT0402
Platinum / Iridium
—
38
0.004 in. (0.102 mm)
2 ft (51 cm)
PT0203
Platinum / Iridium
—
44
0.002 in. (0.051 mm)
3 ft (91 cm)
PT0110
Platinum / Iridium
—
50
0.001 in. (0.025 mm)
10 ft (3 m)
PTP101
Platinum
—
30
0.010 in. (0.25 mm)
1 ft (30 cm)
PTP201
Platinum
—
24
0.020 in. (0.5 mm)
1 ft (30 cm)
PTP401
Platinum
—
18
0.039 in. (1.0 mm)
1 ft (30 cm)
PTP406
Platinum
—
18
0.039 in. (1.0 mm)
0.5 ft (15.2 cm)
PTT0502
Platinum / Iridium
Teflon
36
0.005 in. (0.125 mm)1
2 ft (51 cm)
PTT0203
Platinum / Iridium
Teflon
44
0.002 in. (0.051 mm)1
3 ft (91 cm)
PTT0110
Platinum / Iridium
Teflon
50
0.001 in. (0.025 mm)1
10 ft (3 m)
SS31605
Stainless Steel
—
36
0.005 in. (0.125 mm)
50 ft (15.2 m)
SS31614
Stainless Steel
—
27
0.014 in. (0.36 mm)
30 ft (9.1 m)
SST30407-25 Stainless Steel
Teflon
33
0.007 in. (0.18 mm)3
25 ft (7.6 m)
SST30407-50 Stainless Steel
Teflon
33
0.007 in. (0.18 mm)3
50 ft (15.2 m)
TCJ3050
Thermocouple Glass braid
30
50 ft (15.2 m)
(Iron + Constantan)
Glass braid
30
50 ft (15.2 m)
TCK3050
Thermocouple (Chromel + Alumel)
TI2051
Titanium
—
24
0.020 in. (0.5 mm)
24 ft (7.3 m)2
TI4024
Titanium
—
38
0.040 in. (1.0 mm)
24 ft (7.3 m)2
TGW0325
Tungsten
—
40
0.003 in. (0.075 mm)
25 ft (7.6 m)
TGW0515
Tungsten
—
36
0.005 in. (0.125 mm)
15 ft (4.6 m)
TGW1510
Tungsten
—
26-27
0.015 in. (0.38 mm)
10 ft (3 m)
0.0173 in. (0.44 mm)
20 ft (6 m)4
LAB SUPPLIES
New! Micro coaxial cables (MAXxxxx)
are ideal for microelectrode fabrication and
construction of similar research tools. The dual
shielding eliminates electrical interference
caused by radio frequencies (RF), electrostatic
and microphonics (e.g., bending and vibration.
Available with single or dual (twin) conductors.
Thermocouple wire for tem­per­a­ture mon­i­tor­
ing. TCJ3050, with an iron/con­stan­tan pair of
wires, can be used in inert, vac­u­um, re­duc­ing
or oxidizing atmospheres (iron con­duc­tor may
rust in moist or low temperature ap­pli­ca­tions).
TCK3050 has a chromel/alumel pair, for use
in inert or oxidizing atmosphere at 15001800 °F.
Teflon-coated stainless steel (type 304) wire
(SSTxxxx) is available in 25-ft and 50-ft
lengths. The Teflon coating is 150 micro-in.
thick (4 µm).
Carbon wire (C3005) is a single 30-micron
fiber of electrochemically activated car­bon.
This fiber is especially useful in micro-elec­tro­
chem­i­cal experiments.
Carbon filament wire (CPVCxxxx) is made
of 3,000 microscopic carbon strands in a col­
ored PVC jacket. 45.8 ohms/foot.
Copper ultra-fine wire (CU7T44G) is made
of seven individually insulated 44 AWG (50
micron) conductors twisted together. Total
diameter with insulation is 200 microns.
Resistance: 2.9 Ω/m for all seven wires
together. Dry dielectric strength: 1500V DC.
Platinum/iridium wire — uncoated (PTxxxx)
and Teflon-coated (PTTxxxx) — is an alloy
of 90% platinum and 10% iridium, giving
excellent tensile strength and cor­ro­sion
resistance. Uncoated pure platinum wire
(PTPxxx) is 99.95% pure.
Indium wire (IN1003) is 99.99% pure, with
a melting point of 156.4°C.
Annealed silver wire (AGWxxxx), 99.99%
pure, is available in five diameters; three of
those sizes are also available with a Teflon
coating (AGTxxxx).
Tungsten wire (TGWxxxx), available in
three diameters, is 99.95% pure.
Gold wire (AUWxxxx) is 99.99% pure.
Titanium wire (TIxxxx) is 98.9% pure,
annealed, in two diameters.
Stainless steel wire (SSxxxxx) is type 316.
Catalog No.
Microcoaxial Cables
MAX3820
Tinned Cu Alloy
Coaxial
MAX4020
Tinned Cu Alloy
Twin Coaxial
0.0158x0.024 in. (0.4x0.61mm)
20 ft (6 m)5
*Brown & Sharpe
1
Plus 0.002 in. for Teflon coating
3
Teflon adds 0.00015 in. (4 µm) to diameter4 Impedance: 50 ohm; capacitance: 95 pF/m; resistance: 5 ohm/m
5
Impedance: 100 ohm; capacitance: 54 pF/m; resistance: 1.9 ohm/m
2
Furnished in bundles of 3-ft lengths.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
105
Luer Valve Assortment Kit
A useful kit (above) for building your own liquid flow experiment. It provides the means to start, stop, add, divide and control a flow of liquid or gas.
Included in the kit are over 200 as­sort­ed parts such as one-way and three-way stopcocks, manifolds, Y-connectors, in­jec­tion sites, male and female luer
caps, check valves, syringe-activated check valves, slide clamps, roller clamps, and pinch
clamps. All (except clamps) have a luer fitting for quick and easy con­nect­ing and
14011
Luer Valve Assortment Kit
disconnecting. Includes assorted luer fittings for use with flex­i­ble tubing.
LAB SUPPLIES
Barb-to-Tubing Coupler Assortment Kit
Barb-to-Tubing Assortment Kit (at left) includes three different sizes
of tubing and two boxes with different fittings, T-connectors, elbow
connectors, check valves and plugs.
500890
Barb-to-Tubing Assortment Kit (polypropylene)
Includes 25ft. each of three tubing sizes: 1/16" ID, 1/8" ID, 1/4" ID
Luer-to-Tubing Coupler Assortment Kit Assemble quick-disconnect luer fittings for use with flexible tubing with internal
diame­ters of 1⁄16", 3⁄32", 1⁄8" and 5⁄32". A variety of quick-disconnect connectors can be
quickly made for con­nect­ing small diameter flexible tubing; 3-way connections can
be made with the use of the 3-way luer tee; luer plugs, tees, connectors, bulk-head
mounts, color coding rings, locking nuts, male and female luers—are all in­clud­ed to
enhance the ver­sa­til­i­ty of this kit. The kit has 253 assorted parts and is offered
in two different types of materials. Polypropylene fittings are chemically inert and
resistant to most organic and inorganic solvents. Nylon fittings are strong and can be
bonded with adhesive.
14012
Luer-to-Tubing Coupler Assortment Kit (Polypropylene)
500895
Luer-to-Tubing Coupler Assortment Kit (Nylon) 106
14012
500895
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Pack of 20
Pack of 5
World Precision Instruments
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
3-Port Manifold (5 Female Ports)
Pack of 2
4-Port Manifold (6 Female Ports)
Pack of 2
# 13156-100
Female Luer
Fitting for
1⁄ " ID Tubing
16
Pack of 100
Pack of 100
Pack of 100
Pack of 100
Pack of 100
ID Tubing
Pack of 100
3
/32" ID Tubing
Fitting for
16"
Fitting for
1⁄
32" ID Tubing
3/
Male Luer
Female Luer Fitting Female Luer
for
Fitting for
1⁄ " ID Tubing
5/ " ID Tubing
8
32
Female Luer Fitting
for
Male Luer
# 13158-100
# 13157-100
# 13161-100
# 14059-2
# 14055-2
# 14054-10
1-Way Stopcock,
Luer Slip
Pack of 10
#14038-10
1-Way Stopcock
Luer Lock,
Pack of 10
# 13160-100
Pack of 10
Pack of 15
Pack of 10
Pack of 20
Pack of 10
# 13159-100
Luer Lock
Luer Lock
ID Tubing
Pack of 100
8"
1⁄
Fitting for
Male Luer
# 13162-100
Pack of 100
/32" ID Tubing
5
Fitting for
Male Luer
# 13163-100
Pack of 10
Luer lock,
4-Way Stopcock,
4-Way Luer
Stopcock
4-Way Stopcock,
3-Port Infusion Y
Swivel Thread
Pack of 20
3-Way Stopcock,
# 14058-10
# 14036-15
# 14057-10
# 14048-20
# 14035-10
Pack of 20
4-Port Infusion Y
Swivel Thread
Pack of 60
Roller Clamp
Large Bore
# 14043-20
# 14047-10
Pack of 10
Tubing
Roller Clamp
0.135”/3.4 mm
OD Tubing
3⁄ "
16
# 14041-60
# 13822-10
Female T Luer
LAB SUPPLIES
UK: Tel: +44 (0)1462 424700 • [email protected]
# 3742-20
Pack of 40
Slip Luer Valve
Activated Check
Activated Dual
Check Valve
Pack of 10
Male luer lock
Syringe
Syringe
Check Valve
Injection Site
# 14045-20
# 14044-5
# 14039-10
#14034-40
Pack of 60
Luer Plug
Male/Female
# 14061-60
Pack of 100
Pinch Clamp for 5mm
Tubing
# 14051-100
Pack of 50
Pinch Clamp for
7mm Tubing
# 14040-50
# 14042-100
Slide Clamp
for 2.5 mm
O.D. Tubing
Pack of 100
Parts now sold individually
Buy parts from Luer Valve Assortment Kit 14011 individually
Parts in kit may differ slightly in appearance from those pictured.
107
Cables and Connectors
#3161
#3294
#13620
#3492
#2851
#1358
#5372
#3142
#3417-10
#3517
#3508
#3491
#5371
#5385
#5373
#3670
#5375
LAB SUPPLIES
#13388
#13451
#5374
#13776
#13347
#500082
#13324
#500131
#14254
#15975
#15976
#500081
#13854
#3578
#300040
#300102
#15623
#500256
#13685
#500128
#300344
108
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
#CBL100
#CBL102
#15624
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
LAB SUPPLIES
PART #
APPLICATION/DESCRIPTION
CONNECTOR A
CON­N EC­TOR B
CABLE
LENGTH
1358
Beetrodes
BNC (male)
2 mm pin
3 ft (0.9 m)
2026-10
2 mm socket, unwired (pkg of 10) (Not Shown)
2 mm socket
unwired
none
2851
Standard BNC cable
BNC (male)
BNC (male)
6 ft (1.8 m)
3142
Mini-Banana Adapter
Screw Terminals
Dual Mini-Banana
none
3161
Connector for input to TBM4M and BP-1
DIN (male)
unwired
none
3294
Ground wire for DAM80 probe
Clip
none
3 ft (0.9 m)
3417-10
2 mm plug, unwired (pkg of 10)
2 mm pin
unwired
none
3491
Extension for any 8-pin DIN
DIN (male)
DIN (female)
5 ft (1.5 m)
3492
Connector, adapts WPI transducers to non-WPI equip­ment DIN (female)
unwired
none
3508
Adapts BNC pH electrode to pH meter with
“U.S. Standard” input
BNC (male)
US Standard
none
3517
DAM50, DAM60, DAM70, shielded (two cables/pkg)
Modular phone plug, 4 wire
none
3 ft (0.9 m)
3578
Adapter cable for Ag/AgCl pellets 2 mm pin
none
5 ft (1.5 m)
3670
Double banana plug with solder turret terminals
Dual Banana (male)
Dual Banana (female)
none
5371
Low-noise cable for microelectrode holders
2 mm gold pin
2 mm gold pin
2 ft (0.6 m)
5372
Low-noise cable for microelectrode holders
2 mm gold jack
2 mm gold jack
2 ft (0.6 m)
5373
Low-noise cable for microelectrode holders
2 mm gold pin/jack
2 mm gold pin/jack
2 ft (0.6 m)
5374
Low-noise cable for microelectrode holders
BNC (male)
2 mm gold pin
4 ft (1.2 m)
5375
Low-noise cable for microelectrode holders
BNC (male)
2 mm gold jack
4 ft (1.2 m)
5385
Cable, shielded transducer stock none
none
25 ft (7.6 m)
13324
Adapter
Double-banana (female)
BNC (male)
none
13347
ISO2 (chart recorder adapter)
Double-banana (male)
BNC (female)
none
13388
Electrode adapter for DAM probes
Miniature banana (male)
2 mm jack
none
13451
Adapter: Iso-DAM, Iso-DAM8
BNC (female)
two 2 mm pins
6 in. (15 cm)
13555
Serial Cable (not shown)
DB9 (male)
DB9 (female)
6 ft (1.8 m)
13620
Low-noise cable for microelectrode holders
2 mm gold pin
2 mm gold jack
2 ft (0.6 m)
13685
SP Series pump-to-pump linking cable Modular phone plug
Modular phone plug
7 ft (2.1 m)
13776
Adapts reference electrode to VF4 ground jack
Banana (male)
2 mm jack
none
13854
BNC T-connector, male to:
BNC (female)
BNC (female)
none
14254
BNC Straight Adapter
BNC (female)
BNC (female)
none
15623
Serial cable and adapter, SP Series pump SP Pump
IBM 9-pin “D” connector
5 ft (1.5 m)
15624
Serial cable and adapter, SP Series pump
SP Pump
Macintosh connector
5 ft (1.5 m)
15975
Adapter
2 mm socket
1 mm pin
none
15976
Adapter
1 mm socket
2 mm pin
none
300040
Adapter Extension 2 mm socket
2 mm socket
4 in. (10 cm)
300344
Cable
DIN
BNC
500081
Adapter IBM PC Comm Port
DB9 (male)
DB9 (female)
none
500082
Adapter BNC/RCA MM
BNC (female)
RCA (male)
none
300102
Adapter Extension 2 mm socket
0.31 socket
4 in. (10 cm)
500128
BNC Zero Ohm Terminator
BNC (male)
none
none
500131
Cable for Biode® Snap Electrodes (assembly of 5)
2 mm pin
snap
39 in. (1 m)
500184
Standard BNC Cable
BNC (male)
BNC (male)
10 ft (3 m)
500256
BNC Right Angle Adapter BNC (male)
BNC (female)
none
500257
Standard BNC Cable
BNC (male)
BNC (male)
6 in. (15 cm)
500258
Standard BNC Cable
BNC (male)
BNC (male)
12 in. (30 cm)
500259
Standard BNC Cable
BNC (male)
BNC (male)
18 in. (46 cm)
501670
Coaxial Adapter
Dual Mini-Banana (male)
BNC (female)
none
503301
Cable, Extension
8-pin miniDIN (male)
8-pin miniDIN (female)
10 ft (3 m)
503536
Cable, USB
USB (male)
USB (female)
10 ft (3 m)
CBL100
MiniPhone Patch Cable
3.5 mm MiniPhone plug
3.5 mm MiniPhone plug
6 ft (1.8 m)
CBL102
DAM Series, PM Series
3.5 mmMiniPhone plug
BNC (male)
6 ft (1.8 m)
POWER CORDS
3006
US 120 V, grounded
IEC Connector
3-pin (M) US
6 ft (1.8 m)
3301
European 240 V, grounded
IEC Connector
3-pin (M) European
6 ft (1.8 m)
3302
British 240 V, grounded
IEC Connector
3-pin (M) UK
6 ft (1.8 m)
14088
Australian 240 V, grounded
IEC Connector
3-pin (M) Australian
6 ft (1.8 m)
801824
Indian 240 V, grounded
IEC Connector
3-pin (M) Indian
6 ft (1.8 m)
US: Tel: 941-371-1003 • [email protected]
109
FrameWorks
Non-Magnetic Bases,
Stainless Steel Rods, & Clamps
43
These high quality components are made of
stainless steel and polymer that resist organic
solvents and corrosion. They can be easily
assembled to make a stand-alone setup for
student labs or to make a complicated frame for
research labs.
m
m
LAB SUPPLIES
503081-4 Vertical Surface Mount, M8 Threaded
503085
Large V-base with M8 Thread Mount
502190 Heavy Rectangular Base (with M8 thread mount ....................
and thumbscrew mount), 23×15.6 cm, 4 lb
503083 Light Rectangular Base (with M8 thread mount .......................
and thumbscrew mount), 23×15.6 cm, 0.5 lb
503084
Small V-base with M8 Thread Mount
503070
503071
503072
503073
502191
503075
503076
503077
503088-10
Thumbscrew, M5×20 mm (pkg of 10)
110
Polished Stainless Steel Post, 12mm OD, 25cm long, no thread
Polished Stainless Steel Post, 12mm OD, 50cm long, no thread
Polished Stainless Steel Post, 12mm OD, 75cm long, no thread
Polished Stainless Steel Post, 12mm OD, 25cm long, M8 thread
Polished Stainless Steel Post, 12mm OD, 50cm long, M8 thread
Polished Stainless Steel Post, 12mm OD, 60cm long, M8 thread
Polished Stainless Steel Post, 12mm OD, 75cm long, M8 thread
Polished Stainless Steel Post, 12mm OD, 80cm long, M8 thread
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
14073-4 Open-sided Frame Clamp (pkg of 4)
503082-4Board Frame Clamp, opens up to 8.5 mm (pkg of 4)
503078-4 T-joint Frame Clamp (pkg of 4)
502193-4 Parallel Frame Clamp (pkg of 4)
503079-4 In-line Frame Clamp
(pkg of 4)
38.7 mm
503080-4 Frame Clamp with
Parallel Surface Mount (including two
10-32 mounting screws)
(pkg of 4)
LAB SUPPLIES
503041
Large Clamp with Rod (157 mm), opens up to 85 mm
503042
Medium Clamp with rod (157 mm), opens up to 45 mm
503086
Small Clamp with rod (157 mm), opens up to 16 mm
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
111
We are your new
glassware source!
LAB SUPPLIES
Griffin Beakers, with spout
ASTM E960 Type I
Pack
Case
Catalog # Capacity
of
of
CG-0001
10 mL
12
48
CG-0002
20 mL
12
48
CG-0003
30 mL
12
48
CG-0004
50 mL
12
48
CG-0005 100 mL
12
48
CG-0006 150 mL
12
48
CG-0007 250 mL
12
48
CG-0008 400 mL
12
48
CG-0009 600 mL
6
18
CG-0010 1000 mL
6
18
CG-0011 Starter Set
5
—
y
ualit
q
h
g
hi
ly
ntly extreme eck
e
t
s
i
Cons ware at ices! Ch es —
r
glass etitive p ly sourc o us.
p
comp lab sup e back t
r
othe ou’ll com
y
and
Griffin Beakers, HEAVY-DUTY*, with spout
ASTM E960 Type II
Pack
Case
Catalog # Capacity
of
of
CG-0015 150 mL
12
48
CG-0016 250 mL
12
48
CG-0017 400 mL
12
48
CG-0018 600 mL
6
18
CG-0019
1L
6
18
CG-0020
2L
4
8
CG-0021
4L
1
4
*25-30% heavier than standard weight beakers.
The glassware shown here includes
a sampling of the most popular
pieces. For a complete look at the
whole line, request a copy of WPI's
glassware catalog ...
Quality
LabWare
Graduated Cylinder
Class A, ASTM E1272, E542
Calibrated “To Deliver”
Catalog # Capacity
CG-0150
CG-0151
CG-0152
CG-0153
CG-0154
CG-0155
10 mL
25 mL
50 mL
100 mL
250 mL
500 mL
Graduated Cylinder
Calibrated “To Deliver”
Class B
Catalog # Capacity
CG-0160
5 mL
CG-0161
10 mL
CG-0162
25 mL
CG-0163
50 mL
CG-0164 100 mL
CG-0165 250 mL
CG-0166 500 mL
CG-0167 1000 mL
CG-0168 Starter Set
• Includes plastic bumper guards
• White enamel graduations
u'll
est prices yo it!
...at the low
antee
ar
gu
e
w
ever see —
... or visit WPI's website:
store.wpiinc.com
112
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Erlenmeyer Flasks with Narrow Mouth
ASTM E1404, Type I, Class 1
Pack
Case
Catalog # Capacity Stopper
of
of
CG-0100
25 mL
#0
12
48
CG-0101
50 mL
#1
12
48
CG-0102 125 mL
#5
12
48
CG-0103 250 mL
#6
12
48
CG-0104 500 mL
#7
6
18
CG-0105 1000 mL
#8
6
18
CG-0106
Starter Set
5
—
All WPI glassware meets or exceeds
current ASTM standards
Volumetric Flask with Stoppers
Class A , ASTM E694, E542, and E288
Calibrated “To Contain”
Catalog #
CG-0130
CG-0131
CG-0132
CG-0133
CG-0134
CG-0135
CG-0136
CG-0137
Capacity
10 mL
25 mL
50 mL
100 mL
250 mL
500 mL
1000 mL
2000 mL
Stopper #
9
9
9
13
16
19
22
27
Volumetric Flask with
Calibrated “To Contain”
Capacity
10 mL
25 mL
50 mL
100 mL
250 mL
500 mL
1000 mL
2000 mL
Stoppers
Stopper #
9
9
9
13
16
19
22
27
Class B
Pack of
12
6
6
6
6
6
1
1
LAB SUPPLIES
Catalog #
CG-0139
CG-0140
CG-0141
CG-0142
CG-0143
CG-0144
CG-0145
CG-0146
Pack of
12
6
6
6
6
6
1
1
BEAKER Set
CG-0011
Beaker Starter Set (50, 100, 250, 600 & 1000 mL)
Graduated Cylinder Set
CG-0168
Graduated Cylinder Starter Set (10, 25, 50, 100 & 250 mL)
ERLENMEYER FLASK Set
CG-0106
Erlenmeyer Flask Starter Set (50, 125, 250, 500 & 1000 mL)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
113
Glass Capillaries
Clean, high quality glass for making
micropipette elec­trodes and other
research implements
GLASS, HOLDERS
& ELECTRODES
Standard,
no Filament
WPI offers a wide spectrum of high-quality glass
capillaries. We take pride in our abil­i­ty to ship your
glass order within 48 hours. If you need a special
glass that does not ap­pear in our catalog, please
call us. We will make every effort to provide it for
you.
Fire-Polished glass
capillaries are easier to insert into
microelectrode holders without
damaging the gasket. More
importantly, fire-polished glass
won’t scratch the chloridized wire
used in a recording electrode.
Fire-polishing does not affect the
glass’s mechanical or electrical
properties.
Borosilicate glass capillaries: Close
di­men­sion­al tol­er­anc­es assure mi­cro­elec­trode
uni­for­mi­ty and reproducibility. Available in one-,
two-, three-, five- and seven-barrel con­fig­u­ra­tions;
a complete range of single bar­rel thin-wall siz­es;
and a variety of special con­fig­u­ra­tions. Cap­il­lar­ies
with filaments con­tain a solid fil­a­ment fused to the
inner wall, which speeds filling of electrodes. Cap­il­
lar­ies with or with­out inner filaments are available
for making microelectrodes in a wide range of
diameters .
Single Barrel standard wall thickness cap­
il­lar­ies are offered either with or without in­ner
fil­a­ments for quick filling in a variety of lengths
and diameters. Two usable elec­trodes can be
made from one 6-inch length. ­Borosilicate glass is
Corning N51A.
Thin Wall single barrel capillaries are of­fered
both with or without inner filaments. The con­cen­
tric­i­ty of this material provides ex­cel­lent strength.
Micropipettes made from thin wall capillaries have
fine tips with a short taper.
Note: Because electrode tips erode
when left filled with saline solutions
for long peri­ods, electrodes should
be made and filled im­me­di­ate­ly prior
to use.
114
Standard,
with Filament
Thin Wall,
no Fil­a­ment
Thin Wall,
with Filament
Single-Barrel Standard Borosilicate Glass Tubing
Length
OD
(mm)
ID
(mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
1.0
1.0
1.2
1.2
1.5
1.5
1.0
1.0
1.2
1.2
1.5
1.5
2.0
2.0
1.0
1.0
1.2
1.2
1.5
1.5
2.0
2.0
0.58
0.58
0.68
0.68
0.84
0.84
0.58
0.58
0.68
0.68
0.84
0.84
1.12
1.12
0.58
0.58
0.68
0.68
0.84
0.84
1.12
1.12
Filament
FirePolished
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Quantity
Item
500
500
350
350
225
300
500
500
400
350
300
300
125
200
500
500
350
350
225
225
125
125
1B100F-3
1B100-3
1B120F-3
1B120-3
1B150F-3
1B150-3
1B100F-4
1B100-4
1B120F-4
1B120-4
1B150F-4
1B150-4
1B200F-4
1B200-4
1B100F-6
1B100-6
1B120F-6
1B120-6
1B150F-6
1B150-6
1B200F-6
1B200-6
Thin-Wall Single-Barrel Standard Borosilicate
(Schott Duran) Glass Tubing
OD
(mm)
ID
(mm)
1.0
1.0
1.2
1.2
1.5
1.5
1.0
1.0
1.2
1.2
1.5
1.5
1.0
1.0
1.2
1.2
1.5
1.5
0.75
0.75
0.90
0.90
1.12
1.12
0.75
0.75
0.90
0.90
1.12
1.12
0.75
0.75
0.90
0.90
1.12
1.12
FIL
FirePolished
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Length
Quantity
Item
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
3 in. (76 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
4 in. (100 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
500
500
400
350
225
300
500
500
350
350
225
300
500
500
400
350
225
300
TW100F-3
TW100-3
TW120F-3
TW120-3
TW150F-3
TW150-3
TW100F-4
TW100-4
TW120F-4
TW120-4
TW150F-4
TW150-4
TW100F-6
TW100-6
TW120F-6
TW120-6
TW150F-6
TW150-6
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Patch Clamp Capillary Glass
To select the best patch clamp glass for your exper­
i­ments, we have evaluated available glass types
in terms of the four properties most crucial to
successful patch clamp studies:
types widely used in constructing patch clamp
electrodes. The significant characteristics of each are
as follows:
The softening temperature determines how easily
each glass type can be pulled to the de­sired shape
and the extent to which it can be heat polished.
Glass with a high softening temperature is difficult
to pull and causes unnecessary wear on the
heating element of the puller. This makes it very
hard to make electrodes that are re­pro­duc­ible and
of consistent quality. Patch clamp glass with a low
softening tem­per­a­ture is preferred; however, higher
softening temperature glass is stronger.
Sealability: It is not clear what factors de­ter­mine
the sealing ability of the patch to the glass. Al­most
any glass can form a gigohm seal under the right
conditions. Different glass types vary, how­ev­er, in
how easily they form a seal. It is im­por­tant to select
a patch clamp glass that seals eas­i­ly. Good fire
polish is critical for seal (see DMF1000).
PG10150-4 and PG10165-4 are composed of
Corning #0010 glass, a high lead content (22%
PbO) glass. Its thermal and elec­tri­cal per­for­mance
is between the Schott #8250 and Corning #8161
glass­es described above. It is much more eco­
nom­i­cal than Corning #8161 glass. It has been
found that this glass causes much less alteration in
chan­nel behavior than Corning #8161 and Schott
#8250 glass (Furman and Tanaka, Biophys. J. 53,
p287, 1988).
GLASS, HOLDERS
Electrical properties determine how much noise
the glass is likely to produce in recording sit­u­a­
tions. The lower the product of dielectric con­stant
times the loss factor, the smaller the equiv­a­lent
noise current the glass will produce (Rae and Levis,
Methods in En­zy­mol­o­gy, 207, p67, 1992). Patch
clamp glass with good elec­tri­cal properties is critical
especially in single-chan­nel recording.
PG52151-4, PG52152-4 and PG52165-4 are
prepared from Schott #8250 glass (equivalent to
Corn­ing #7052), one of the most widely used patch
clamp­ing glasses. This is a specially formulated
boro­sil­i­cate glass with a softening temperature that
is 110°C lower than regular borosilicate glass (Corn­
ing 7740, or Pyrex). It has excellent sealing prop­er­
ties for most cells. Electrical properties are also very
good.
Patch clamp capillaries do not have mi­crofil­a­ments.
Leachable components: Substances leached from
glass can alter channel behavior. Since dif­fer­ent
channels are sen­si­tive to different glass com­po­nents,
it is best to record one type of chan­nel with several
different kinds of pipette glass to elim­i­nate any
artifact due to the glass.
PATCH CLAMP CAPILLARY GLASS
Catalog#
PG52151-4
PG52165-4
PG10150-4
PG10165-4
Glass Type OD/ID (mm)
#8250
#8250
#0010
#0010
1.5/1.0
1.65/1.1
1.5/0.75
1.65/1.1
Dielectric
Constant
4.9
4.9
6.7
6.7
Softening
Point °C
720°
720°
625°
625°
Quantity
100
100
100
100
WPI offers capillary tubing made from two glass
Glass Handling Forceps
& ELECTRODES
Ever had difficulty picking up a glass capillary? Special tips on these forceps solve the
problem, holding glass firmly without risk of breakage. They also keep the glass clean
and avoid contamination from skin oils.
77020
Glass Handling Forceps
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
115
Multi-Barrel Glass Capillaries
GLASS, HOLDERS
& ELECTRODES
Two-Barrel
Multi-barrel configurations are designed
es­pe­cial­ly for microiontophoresis.
Because the capillaries are fused together
during man­u­fac­ture, you will not need to
twist them while pulling to seal the tips
together. An inner fil­a­ment in each barrel
makes filling easy and fast.
Also see PolyFil for a novel
way to connect multi-barrel
pipettes
Three-Barrel
Five-Barrel
Seven-Barrel
Multi-Barrel Borosilicate Glass Tubing with Filaments
Length
Description
OD/ID (mm)
Filament
Quantity
Item
4 in. (102 mm)
4 in. (102 mm)
4 in. (102 mm)
4 in. (102 mm)
4 in. (102 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
6 in. (152 mm)
Two-Barrel
Three-Barrel
Five-Barrel
Seven-Barrel
Seven-Barrel
Two-Barrel
Three-Barrel
Five-Barrel
Seven-Barrel
1.5/0.84
1.2/0.68
1.2/0.68
1.2/0.58
1.2/0.68
1.5/0.84
1.2/0.68
1.2/0.68
1.0/0.58
4
4
4
4
4
4
4
4
4
100
100
65
60
75
100
100
65
60
2B150F-4
3B120F-4
5B120F-4
7B100F-4
7B120F-4
2B150F-6
3B120F-6
5B120F-6
7B100F-6
Special Configuration
Borosilicate Capillaries
Septum Theta offers superior cell impalement. The nat­u­ral
bevel resulting from the prominent spear-like pro­jec­tion of the
septum gives microelectrodes a sharp, spear-point tip. This
style has low resistance for use as a single mi­cro­elec­trode, and
it can be used to make superior dou­ble-tipped microelectrodes
with low trans-tip coupling. The nat­u­ral bevel of Septum Theta
Septum Theta
Piggyback
also sig­nif­i­cant­ly increases the effective tip cross-section. As
supplied, the width of the sep­tum is ap­prox­
i­mate­ly 0.2 mm; wall thick­ness is ap­prox­i­
Special Configuration Borosilicate Glass Tubing
mate­ly 0.2 mm.
Description
OD/ID (mm)
Length
Quantity
Item
Piggyback glass consists of a pair of boro­
sil­i­cate capillaries fused together during
Septum Theta
1.5/1.02
6 in. (152 mm)
100
TST150-6
man­u­fac­ture. One barrel is larger than the
1.51/0.84
other, and both have inner filaments for
Piggyback
4 in. (102 mm)
50
PB150F-4
0.75/0.35
quick filling. Pig­gy­back glass makes it simple
1.51/0.84
to fab­ri­cate two-barrel elec­trodes with a
Piggyback
6 in. (152 mm)
50
PB150F-6
0.75/0.35
significant tip diameter differential.
Borosilicate glass rod
1.0 mm diameter — for
making tools, probes, tips
Borosilicate Glass Rod
Description
OD (mm)
Length
Quantity
Item
Glass Rod
Glass Rod
1.0
1.0
4 in. (102 mm)
6 in. (152 mm)
500
500
GR100-4
GR100-6
Micropipette
Storage Jar
E210
E212
E215
E220
Replacement Parts
1965 Foam Ring for 0.75 - 1.0 mm glass
1966 Foam Ring for 1.2 - 1.5 mm glass
1967 Foam Ring for 2.0 mm glass
Stores up to 30 micropipettes, filled or
unfilled, up to three inches in length. A
gentle sliding action inserts or removes
pipettes without damage to the delicate tips.
116
Storage Jar for 1.0 mm OD Mi­cropi­pettes
Storage Jar for 1.2 mm OD Mi­cropi­pettes
Storage Jar for 1.5 mm OD Mi­cropi­pettes
Storage Jar for 2.0 mm OD Mi­cropi­pettes
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
TM
Borosilicate glass micropipettes
Plain Shank or
Luer Fittings
Shank
Length
Glass O.D.
Filament
Fire
Polished
Catalog #
PLAIN
0.1 µm
0.2 µm
0.3 µm
0.4 µm
0.5 µm
1 µm
2 µm
5 µm
10 µm
10 µm
30 µm
—
—
—
—
—
—
—
—
—
—
—
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.14 mm A203XV glass *
1.0 mm Thin-Wall
Yes
Yes
Yes
Yes
Yes
No
No
No
No
No
No
No
No
No
No
No
Yes
Yes
Yes
Yes
Yes
Yes
TIP01TW1F
TIP02TW1F
TIP03TW1F
TIP04TW1F
TIP05TW1F
TIP1TW1
TIP2TW1
TIP5TW1
TIP10TW1
TIP10XV119
TIP30TW1
LUER
0.1 µm
0.2 µm
0.3 µm
0.4 µm
0.5 µm
1 µm
2 µm
5 µm
10 µm
30 µm
—
—
—
—
—
—
—
—
—
—
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
Yes
Yes
Yes
Yes
Yes
No
No
No
No
No
—
—
—
—
—
—
—
—
—
—
TIP01TW1F-L
TIP02TW1F-L
TIP03TW1F-L
TIP04TW1F-L
TIP05TW1F-L
TIP1TW1-L
TIP2TW1-L
TIP5TW1-L
TIP10TW1-L
TIP30TW1-L
LUER/SILANIZED
5 µm
5 µm
10 µm
10 µm
30 µm
30 µm
1 inch
2 inch
1 inch
2 inch
1 inch
2 inch
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
1.0 mm Thin-Wall
No
No
No
No
No
No
—
—
—
—
—
—
TIP5TW1LS01
TIP5TW1LS02
TIP10TW1LS01
TIP10TW1LS02
TIP30TW1LS01
TIP30TW1LS02
● Schott Duran borosilicate glass
● 0.5 micrometer and smaller ID
micropipettes include an internal glass
fiber for easy filling
● Tip inner diameter tolerance ±20%
● Short taper yields high strength
● Nominal length ≈ 50 mm
● OD:ID = 1.33:1
● Standard capillary outer diameters are
1.0 mm (thin-wall) or 1.14 mm
(pack
of 10)
GLASS, HOLDERS
Eliminate the cost and trouble of making
your own micropipettes — WPI can
quickly supply your need for consistently
sized pre-pulled glass micropipettes for
injection of dyes or proteins into cells,
oocytes and for many other biomedical
laboratory applications. Tip diameters (ID)
range from 0.1 to 10 micrometers.
Tip
I.D.
Shank
* 10 µ (ID), 1.14 mm capillary pipettes are for use in WPI’s Nanoliter 2000.
● Every pipette individually tested and
inspected
● Vacuum packed
Silanization waterproofs the glass to retard water
when inserting into cell. This will not let the outside fluid run down the pipette and get inside so
easily.
Micro Cannula
l 0.4mm O.D., 0.2mm I.D. tubing
l Autoclavable
l Biocompatible Perfluorocarbon tubing material
KZ1101
Micro Cannula, 3-inch
& ELECTRODES
µTip Sampler Assortments
TIPMIX01-05 Two each, 0.1, 0.2, 0.3, 0.4, 0.5 µm ID, plain shank
TIPMIX05-10 Two each, 0.5, 1, 2, 5, 10 µm ID, plain shank
TIPMIX01-05-L Two each, 0.1, 0.2, 0.3, 0.4, 0.5 µm ID, Luer
TIPMIX05-10-L Two each, 0.5, 1, 2, 5, 10 µm ID, Luer
Silanized Tips (Luer Shank)
This micro cannula is ideal for placement in the carotid
or femoral artery of mice, rats, and other
small animal blood vessels. It can be used
with a pressure transducer (WPI’S BLPR2) for
blood pressure measurement, or in conjunction with a
micro-syringe injection system (like WPI’s UMPIII or MMP
pumps). The incorporated standard female luer fitting makes
connecting to existing experimental plumbing quick and easy. The
cannula is provided with a contoured-tip stainless steel stylet (trocar)
to facilitate placement using established techniques. A movable “shoulder”
ring provides a tie-in point to prevent accidental removal. The cannula may
be left in place for 2 hours or more, and with proper care and cleaning, may be
re-used multiple times. Instructions for use included.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
117
MicroFil™
The MicroFil’s tip elas­tic­i­ty is sturdy and very
flexible though not un­break­able. Since it is
more flex­i­ble than stainless steel nee­dles,
moderate bending will not block or dam­age
the MicroFil needle. The com­bi­na­tion of plas­tic
and fused silica in the MicroFil tip is sturdier
than plastic tips, allowing easy and repeated
in­ser­tions into mi­cropi­pettes. MicroFil’s luer
fitting allows easy coupling to syringes and
syringe filters.
MF34G-5
MicroFil, 34 ga., 67 mm long (pkg of 5)
MF28G-5
MicroFil, 28 ga., 97 mm long (pkg of 5)
MF28G67-5 MicroFil, 28 ga., 67 mm long (pkg of 5)
U.S. PATENT 5,294,325
& ELECTRODES
GLASS, HOLDERS
WPI’s MicroFil™ fills micropipettes easily and
reli­ably. Its long and fine tip allows you to start
the filling very close to the pi­pette tip, elim­i­
nat­ing both air bub­ble for­ma­tion and clog­ging
due to the wash­ing down of dust particles. The
trans­par­ent amber MicroFil needle is con­struct­
ed from a com­bi­na­tion of plastic and fused
silica — no met­al com­po­nents are used. The
MicroFil needle can be stored for days with the
filling solution inside without clogging.
1-5 pkgs
6-10 pkgs
Custom MicroFil
All MicroFil products, including custom orders, can be shipped immediately. Custom orders for special
needs can be made using nine sizes of MicroFil tubing in lengths up to 50 cm — except for CMF90UxxL
which has a maximum length of 10 cm because of its high resistance to flow. Quantity discounts
available. Specify length when or­der­ing by in­sert­ing the length (in centimeter in­cre­ments) into
the catalog number in place of the XX’s.
Nonmetallic
syringe needle for
filling micropipettes
PolyFil
CMF20GxxL
CMF22GxxL
CMF23GxxL
CMF26GxxL
CMF28GxxL
CMF31GxxL
CMF34GxxL
CMF35GxxL
CMF90UxxL
MicroFil, 20 Gauge, 700 µm ID, 850 µm OD (pkg of 4)
MicroFil, 22 Gauge, 530 µm ID, 700 µm OD (pkg of 4)
MicroFil, 23 Gauge, 530 µm ID, 665 µm OD (pkg of 4)
MicroFil, 26 Gauge, 320 µm ID, 430 µm OD (pkg of 4)
MicroFil, 28 Gauge, 250 µm ID, 350 µm OD (pkg of 4)
MicroFil, 31 Gauge, 100 µm ID, 238 µm OD (pkg of 4)
MicroFil, 34 Gauge, 100 µm ID, 164 µm OD (pkg of 4)
MicroFil, 35 Gauge, 75 µm ID, 144 µm OD (pkg of 4)
MicroFil, approx. 36 Gauge, 20 µm ID, 90 µm OD (pkg of 4)
™
Multi-barrel
micropipette
coupling kit
MicroFil™
inserted into
each barrel
A drop of hot glue
provides a secure,
leak-free seal.
PolyFil allows easy and secure coupling of a multi-barrel micropipette to
a pres­sure source. Cou­pling is achieved by bonding tem­per­a­ture-re­sis­tant
and flexible MicroFil to the capillary tube with hot melt ad­he­sive. The
luer end of each MicroFil is connected to PVC tubing (200 PSI rated). Kits
also include a five-port manifold that allows use of a sin­gle PV800 Series
PicoPump to drive up to six mi­cropi­pette barrels in­de­pen­dent­ly by switch­
ing on only the barrels to be in­ject­ed. All con­nec­tions are locking luers —
pres­sure safe and convenient.
Pipette pulled from
multi-barrel glass capillary
118
Kit in­cludes: 1 pi­pette holder/han­dle, plastic; 7 pieces MF28G MicroFil;
7‑piec­es tubing with male luer lock fittings; 1 flow-thru man­i­fold with five
luer lock ports; 1 hot melt glue gun(110V only); 3 glue sticks.
5440
13316
Poly­Fil Multi-Barrel Mi­cropi­pette Coupling Kit
Mini Glue Gun and (3) glue sticks World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Ag/AgCl Half-Cells
EP05
New, improved sintered pellets with lower resistance and high strength.
Stable and well balanced in the presence of current, these small and
inexpensive half-cells are easy to work with as bath electrodes.
EP08
0.5 mm dia.
2 cm long
0.8 mm dia.
2 cm long
Ag Wire 40 mm long
EP1
EP4
EP2
1 mm dia.
3 mm long
4 mm dia.
1 mm thick
2 mm dia.
4 mm long
Ag Wire 70 mm long
Ag Wire 5 cm long
Ag wire 12 mm long
EP12
EP8
12 mm dia.
1 mm thick
8 mm dia.
1 mm thick
Ag wire 12 mm long
Ag wire 12 mm long
Reference Cell with 1.5 m lead
Reference Cell, 1.5 m lead, 2 mm pin
Reference Cell with 2.0 mm pin
Reference Cell with female connector
Reference Cell with epoxy body, 4.5 mm diam x 50 mm
Reference Cell with glass body, 4.0 mm diam x 75 mm
Reference Cell with glass body, 4.0 mm diam x 50 mm
Reference Cell with glass body, 1.5 mm diam x 50 mm
Ag/AgCl Electrode 0.5 mm diam x 20 mm
Ag/AgCl Electrode 0.8 mm diam x 20 mm
Ag/AgCl Electrode 1.0 mm diam x 2.5 mm
Ag/AgCl Electrode 2.0 mm diam x 4 mm
Ag/AgCl Electrode 4.0 mm diam x 1 mm
Ag/AgCl Electrode 8.0 mm diam x 1 mm
Ag/AgCl Electrode 12.0 mm diam x 1 mm
Adapter Cable for Ag/AgCl Pellets
RC1 / RC1T
25.4 mm
Insulator
13 mm
3.9 mm DIA.
15.9 mm
4 mm DIA.
8.5 mm
#24 AWG
insulated wire,
4 ft
4 mm DIA.
RCI has an unterminated
lead. RC1T is terminated
with a 2 mm pin.
2 mm DIA.
Ag/AgCl Pellet
RC2
25
RC2F
mm
25
mm
75
mm
mm
& ELECTRODES
50
2 mm Jack
to Ag/AgCl Pellet
2 mm DIA. Gold Plated Pin
to Ag/AgCl Pellet
glass body
epoxy body
2.5 mm diam. Ag/AgCl pellet
3.5 mm diam. Ag/AgCl pellet
RC3
4.5 mm diam.
4 mm diam.
RC4
25
53
mm
1.5 m insulated lead
mm
50
mm
glass body
glass body
2.5 mm diam. Ag/AgCl pellet
1 mm diam. Ag/AgCl pellet
4 mm diam.
3 mm
RC5
1.5 mm diam.
RC6
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
GLASS, HOLDERS
RC1
RC1T
RC2
RC2F
RC3
RC4
RC5
RC6
EP05
EP08
EP1
EP2
EP4
EP8
EP12
3578
Ag Wire 40 mm long
US: Tel: 941-371-1003 • [email protected]
119
Micropipette Holders & Half-Cells
WPI’s microelectrode holder-half-cells couple fluid-filled glass
micropipettes to high input impedance amplifiers. A Ag/AgCl pellet (or
a silver wire) molded into the holder body provides stable potential.
Electrical connection is made via male 2 mm pins or female 2 mm
sockets. The pipette may be mounted axially or at right angles to the
holder. Pipettes are held with screw-caps or rubber gaskets (without
caps). Filling WPI microelectrode holders with electrolytes containing
chloride results in stable electrode potential. Suitable electrolytes
include KCl, NaCl and CaCl2. Holders are supplied for standard WPI
single capillary tubing of 1.0, 1.2, 1.5 and 2.0 mm outside diameters.
(Call WPI regarding custom designs for other glass diameters.) The
holder style you select will depend on your experimental application,
space, and instrumentation.
35.7 mm
35.7 mm
2 mm Pin
to Ag Wire
2 mm Pin
to Ag/AgCl Pellet
9.5 mm DIA.
9.5 mm DIA.
APEH1
APEH2
35.
GLASS, HOLDERS
& ELECTRODES
Hints for selecting and ordering micropipette holders
1. Determine the required electrical connection on the holder: for
example, if you wish to connect the holder to a 2 mm pin you should
select a holder equipped with a 2 mm jack. Most WPI probes require a
holder equipped with a 2 mm jack.
25.4 mm
34.9 mm
9.5 mm DIA.
25.4 mm
9.5 mm DIA.
2. Decide on the required alignment of the electrical connection:
either in-line with the glass pipette, or at a right angle to it. Space
considerations in your experimental setup and requirements imposed
by other pieces of equipment typically determine which alignment is
appropriate.
O-Ring
O-Ring
2 mm Jack to Ag Wire
2 mm Pin
to Ag Wire
EHB1
EHBF
3. Determine if you want to hold the glass pipette by a rubber gasket
(e.g., MEH1S) or a screw-cap (e.g., MEH3S). Rubber gaskets offer easier
insertion and removal of glass pipettes whereas screw-caps provide
more secure mounts for micropipetters.
4. Choose a holder with either a silver wire or a silver/silver chloride
pellet for the metal/liquid coupling. Silver/silver chloride pellets provide
a more stable low-noise baseline which is important for low-noise DC
recording. Pellets require the glass pipette and holder to be free of air
bubbles to achieve a good connection. Silver wire holders are durable
and are easier to use when the holder is equipped with a pressure port
because the fluid in the pipette does not have to be filled to the top of
the pipette to achieve a good electrical connection
5. Choose a holder equipped with a pressure port only when you
want to pressure inject liquid from the pipette. Two types of ports are
available: 2.0 mm O.D. and standard “syringe-style” luer. The luer port
is often recommended because it makes assembly and disassembly
much easier. Quick-connect luer fittings for four common sizes of tubing
(1/16", 3/32", 1/8", 5/32" I.D.) are included with each luer-equipped holder.
63.5 mm
∅1.2 mm
∅1.2 mm
MEH6RF/SF is designed primarily for use with the Model 900A
Micropressure System; EHB1 for use in electrode beveling; and
MEH3SW for microtitration of chloride with a silver wire as the
electrode and a solution of silver nitrate filling the holder. MPH models
do not contain Ag/AgCl half-cells and are used for pressure injection
of substances through microelectrodes. PicoNozzle, used for pressure
injection with PV800 Series PicoPumps, includes an MPH6S holder —
which may also be used to couple a micropipette to a syringe. APEH
models are also designed for use in pressurized injection procedures.
MALE “LUER” PORT
∅1.5 mm
∅ 6.35 mm
FOIMPH
Holder for P-5 and P-7
multibarrel glass (see
al
page 130)
ion
126 mm
6. Some non-WPI preamplifiers or headstages cannot be mounted
on micromanipulators. In such cases, a holder equipped with a rod
(e.g., MEH8) permits the holder to be conveniently mounted on a
micromanipulator.
7. Finally, remember to specify the O.D. of the glass you will be using
when you place your order.
63.5 mm
t
Op ndle
Ha
∅ 6.35 mm
∅1.5 mm
9.5 mm
6.3 mm DIA.
20.7 mm
30 mm
2 mm Pin
to Ag/AgCl Pellet
Female “Luer” Port
for 3 mm OD glass
9.5 mm DIA.
20.7 mm
MBMPH5-7
6.3 mm DIA.
45°
MEH145
20.7 mm
Gasket
2 mm Pin
to Ag/AgCl Pellet
2 mm Jack
to Ag/AgCl Pellet
45°
120
FOIMPH-LF
MEH1F45
4.7 mm DIA.
MEH1R
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
MICROELECTRODE HOLDERS
Connector
Half-Cell
Pressure Port
Screw
Cap
APEH1xx
APEH2xx
EHB1xx
EHBFxx
FOIMPHxx
FOIMPH-LFxx
MBMPH5-7xx
MEH145xx
MEH1F45xx
MEH1Rxx
MEH1RFxx
MEH1Sxx
MEH1SFxx
MEH2Rxx
MEH2RFxx
MEH2RFWxx
MEH2RWxx
MEH2Sxx
MEH2SFxx
MEH2SFWxx
MEH2SWxx
MEH345xx
MEH3F45xx
MEH3FW45xx
MEH3Rxx
MEH3RFxx
MEH3RFWxx
MEH3RWxx
MEH3Sxx
MEH3SBWxx
MEH3SFxx
MEH3SFWxx
MEH3SWxx
MEH3W45xx
MEH6RFxx
MEH6RFWxx
MEH6SFxx
MEH6SFWxx
MEH7xx
MEH7Wxx
MEH8xx
MEH900Rxx
MEH900Sxx
MPH1xx
MPH3xx
MPH4xx
MPH6Pxx
MPH6Rxx
MPH6Sxx
Right
Right
Straight
Straight
Straight
Straight
---45°
45°
Right
Right
Straight
Straight
Right
Right
Right
Right
Straight
Straight
Straight
Straight
45°
45°
45°
Right
Right
Right
Right
Straight
Straight
Straight
Straight
Straight
45°
Right
Right
Straight
Straight
Right
Right
Right
Right
Straight
—
—
—
Right
Right
—
Male
Male
Male
Female
Fiber Optic
Fiber Optic
None
Male
Female
Male
Female
Male
Female
Male
Female
Female
Male
Male
Female
Female
Male
Male
Female
Female
Male
Female
Female
Male
Male
Banana
Female
Female
Male
Male
Female
Female
Female
Female
Male
Male
Male
Male
Male
None
None
None
Male
Male
None
Pellet
Wire
Wire
Wire
None
None
None
Pellet
Pellet
Pellet
Pellet
Pellet
Pellet
Pellet
Pellet
Wire
Wire
Pellet
Pellet
Wire
Wire
Pellet
Pellet
Wire
Pellet
Pellet
Wire
Wire
Pellet
Wire
Pellet
Wire
Wire
Wire
Pellet
Wire
Pellet
Wire
Pellet
Wire
Pellet
Pellet
Pellet
None
None
None
Pellet
Wire
None
No Port
No Port
No Port
No Port
No Port
Male Luer
Female Luer
No Port
No Port
No Port
No Port
No Port
No Port
Male Luer
Male Luer
Male Luer
Male Luer
Male Luer
Male Luer
Male Luer
Male Luer
No Port
No Port
Port
No Port
No Port
No Port
No Port
No Port
No Port
No Port
No Port
No Port
No Port
2.0-mm Port
2.0-mm Port
2.0-mm Port
2.0-mm Port
2.0-mm Port
2.0-mm Port
No Port
2.0-mm Port
2.0-mm Port
Female Luer
Male Luer
2.0-mm Port
Female Luer
Female Luer
Female Luer
2 Caps
2 Caps
N/A
N/A
w/Cap
w/Cap
w/Cap
No Cap
No Cap
No Cap
No Cap
No Cap
No Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
w/Cap
*
Designed for
WPI Products
MBS, 48000
MBS, 48000
MBS, 48000
MBS, 48000
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
ISO-80, ISO-DAM8A
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
705, 773, 767, 721, FD223
& ELECTRODES
Electric
Connection
Angle
GLASS, HOLDERS
Replace XX with
glass diameter
Order Number
900A
900A
Piconozzle Kit (5430-XX)
Piconozzle Kit (5430-XX)
Piconozzle Kit (5430-XX)
* Specify O.D. of glass (1.0, 1.2, 1.5 or 2.0 mm) by replacing XX in the Order Number with 10, 12, 15 or 20.
Handles and Accessories (not included)
Handle #2505 is for use with WPI manipulators. The smaller diameter handle #5444 is required for use with Narishige and Zeiss manipulators.
2505
5444
GO1-100
GO2-100
GO3-100
GO4-100
1571 /4-in
(6.3 mm) diameter handle
(4.8 mm) diameter handle
Replacement gasket 1.0 mm, Package of 100
Replacement gasket 1.2 mm, Package of 100
Replacement gasket 1.5 mm, Package of 100
Replacement gasket 2.0 mm, Package of 100
Clear Silicone Rubber Sealant (‑4.7 oz‑)
1
/16-in
3
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
121
33.5 mm
20.7 mm
23.9 mm
27.2 mm
23.9 mm
Gasket
Gasket
2 mm Jack
to Ag/AgCl Pellet
6.3 mm DIA.
2 mm Jack
to Ag/AgCl Pellet
2 mm Pin
to Ag/AgCl Pellet
MEH1RF
4.7 mm DIA.
4.7 mm DIA.
MEH1S
27.2 mm
27.2 mm
6.3 mm DIA.
MEH1SF
MEH2R
36.7 mm
27.2 mm
6.3 mm DIA.
2 mm Pin
to Ag/AgCl Pellet
Male
“Luer”
Port
27.2 mm
6.3 mm DIA.
2 mm Jack
to Ag/AgCl Pellet
Male “Luer” port
2 mm Jack
to Ag Wire
Male
“Luer”
Port
6.3 mm DIA.
29 mm
Male
“Luer”
Port
Male
“Luer”
Port
2 mm Pin
to Ag Wire
2 mm Pin
to Ag/AgCl Pellet
6.3 mm DIA.
MEH2RF
MEH2RW
MEH2RFW
29 mm
MEH2S
6.3 mm DIA.
27.2 mm
36.7 mm
27.2 mm
6.3 mm DIA.
Male “Luer” Port
Male
“Luer”
Port
2 mm Jack
to Ag/AgCl Pellet
6.3 mm DIA.
MEH2SF
6.3 mm DIA.
27.2 mm
GLASS, HOLDERS
& ELECTRODES
6.3 mm DIA.
2 mm Jack to Ag Wire
2 mm Pin
to Ag/AgCl Pellet
Male
“Luer”
Port
2 mm Pin to Ag Wire
45°
27.2 mm
27.2 mm
27.2 mm
MEH345
MEH2SW
MEH2SFW
6.3 mm DIA.
2 mm Jack
to Ag Wire
2 mm Jack
to Ag/AgCl Pellet
45°
45°
MEH3FW45
MEH3F45
27.2 mm
2 mm Pin
to Ag/AgCl Pellet
6.3 mm DIA.
MEH3R
2 mm Jack
to Ag/AgCl Pellet
6.3 mm DIA.
MEH3RF
27.2 mm
36.7 mm
6.3 mm DIA.
27.2 mm
2 mm Pin
to Ag Wire
2 mm Jack
to Ag Wire
6.3 mm DIA.
122
MEH3RFW
6.3 mm DIA.
MEH3RW
2 mm Pin
to Ag/AgCl Pellet
MEH3S
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
29 mm
29 mm
35.2 mm
36.7 mm
27.2 mm
27.2 mm
6.3 mm DIA.
2 mm Jack
to Ag/AgCl Pellet
Mini-Banana Plug
to Ag Wire
MEH3SBW
27.2 mm
6.3 mm DIA.
6.3 mm DIA.
2 mm Jack
to Ag Wire
2 mm Pin to Ag Wire
6.3 mm DIA.
MEH3SFW
MEH3SF
29 mm
33.6 mm
29 mm
6.3 mm DIA.
29 mm
6.3 mm DIA.
6.3 mm DIA.
6.3 mm DIA.
2 mm Pin
to Ag Wire
2 mm Jack
to Ag/AgCl Pellet
45°
2 mm Jack
to Ag Wire
2.0 mm O.D. Port
2 mm Jack
to Ag/AgCl Pellet
2.0 mm O.D. Port
2.0 mm O.D. Port
MEH6RF
MEH3W45
l
na
tio
Op ndle
Ha
29 mm
123.7 mm
6.3 mm DIA.
2.0 mm O.D. Port
2 mm Pin
to Ag Wire
6.3 mm DIA.
MEH7
36.7 mm
6.3 mm DIA.
2.0 mm O.D. Port
2 mm Pin
to Ag/AgCl Pellet
MEH8
l
na
tio e
Opandl
H
123.7 mm
27.2 mm
Female
“Luer”
Port
MEH900S
Male
“Luer”
Port
MPH1
9.5 mm DIA.
6.3 mm DIA.
42 mm
MPH3
42 mm
Optional
Handle
27.2 mm
MPH4
6.3 mm DIA.
MPH6P
Female
Locking “Luer”
Port
Female
Locking “Luer”
Port
Female
Locking “Luer”
Port
6.3mm DIA.
4.7mm DIA.
2 mm PIN
to Ag WIRE
2 mm PIN
to Ag WIRE
6.3 mm DIA.
6.3 mm DIA.
2.0 mm O.D. Port
42 mm
2.0 mm O.D.
Port
l
na
tio e
Opandl
H
30 mm
2 mm Pin
to Ag/AgCl Pellet
MEH900R
123.7 mm
MEH7W
126 mm
27.2 mm
29 mm
6.3 mm DIA.
2 mm Pin
to Ag/AgCl Pellet
6.3 mm DIA.
MPH6R
6.3 mm DIA.
MPH6S
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
123
& ELECTRODES
33.6 mm
123.7 mm
27.2 mm
2 mm Pin
to Ag/AgCl Pellet
6.3 mm DIA.
l
na
tio e
Opandl
H
2.0 mm O.D.
Port
2.0 mm O.D.
Port
2 mm Jack to Ag Wire
MEH6SFW
l
na
tio e
Opandl
H
123.7 mm
27.2 mm
27.2 mm
MEH6SF
MEH6RFW
GLASS, HOLDERS
33.6 mm
MEH3SW
Microforges
DMF1000 Complete Microforge System.................................................................................................................................126
MF200 Complete Microforge System.......................................................................................................................................125
Microbevelers
48000 Microbeveler.....................................................................................................................................................................129
MBS MicroBeveler System...........................................................................................................................................................129
MICROFORGES,
PULLERS, BEVELERS
1300M Microelectrode Beveler..................................................................................................................................................128
Impedance Meters
Omega-Tip-Z Millivolt and Megohm Meter............................................................................................................................. 81
Pullers
PMP-107 Programmable Multipipette Puller.........................................................................................................................130
Application Guide
P/N
Description
Application
Features
DMF1000
Complete Microforge System;
includes Programmable Digital
Controller and Microscope
Fabrication of special shapes of glass
micropipettes, e.g., pressure polishing of
patch clamp pipettes and making of holding
pipettes. Unique for pipette tip calibration and
microinjection pipettes.
Most sophisticated and only microforge on
the market with built-in pressure polishing
capability. Programmable controller with
10 user-selectable memory storage (of heat
and time) for reproducibility.
MF200
Complete Microforge System, with
analog controller
Use to fire polish glass micropipettes and prepare
special shapes
Comes with W30S Microscope, a 40x
objective and three filament sizes. Uses
exclusive Kohler illuminator instead of
industry standard frosted glass illuminator
for less glare and sharper image.
48000
Microbeveler System
Bevels micropipette tips larger than 1
micron at 5400 rpm for applications such as
microinjection.
Solid surface unit beveler with rotating disk.
Includes basic kit with abrasive alumina
lapping film.
1300M
Microelectrode Beveler
and Start-up kit
Glass micropipette beveler for submicron tips.
Includes M3301R Manipulator and M10
magnetic stand.
Includes 48000 Microbeveler, NOVA
illuminator, fiber optic cable, PZMIII stereo
zoom microscope with special tilt base,
two 20X eyepieces, one 20x eyepiece
with reticle, tool holder and pipette holder
FOIMPH
MBS
Microelectrode Beveler System
Bevels micropipette tips larger than 1
micron at 5400 rpm for applications such as
microinjection.
MES
Microelectrode Beveler System
Glass micropipette beveler for submicron tips.
Includes 1300M Microelectrode Beveler,
1350M Micropositioner and magnetic
stand, OmegaZ, 5052 Steel base plate,
5468 adapter and 3485 Ringstand mount
Omega-Z
Omega-Tip-Z with Probe and Holder
Measures impedance of metal and glass capillary
microelectrodes.
Comes with probe, probe handle and
cables.
Produces 4- or 7-barrel glass pipettes
Equipped with microcomputer, pneumatic
pulling arm, pneumatic rotator, optical­-digital ruler. Pulling process is programmable and under control of a preset sequence.
PMP-107
124
Programmable Multipipette Puller
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Sometimes the simplest designs work best.
MF200 Microforge
MICROFORGES, PULLERS,
The MF200 Microforge is a versatile instrument
designed specifically for the fabrication of
glass micropipettes and other related tools. The
system was developed in collaboration with Dr.
Ming Li of the Department of Pharmacology,
University of South Alabama. It is perfect for
patch pipette tip polishing, tip size reduction,
contact stretching, in vitro fertilization pipette
production and a variety of other pipette
configurations. The MF200 simple, reliable and
is priced economically.
Features of the MF200
The MF200 system includes: An easy to use
analog temperature controller, a specially
configured WPI model W30S research grade
compound microscope, 40x long-working
distance objective and 10x eyepiece. 40x
magnification is essential when polishing
pipettes as small as half a micron (0.5 µm)
in diameter. Compared to a conventional
40x objective, the long working distance
objective reduces the danger of damage to
the pipette and/or objective lens during the
polishing process. It is also the only commercial
microforge using the Kohler illuminator and
Abbe condenser for illumination. This provides
less glare and sharper image of the pipette than
frosted glass illuminator, which was used on all
of the other commercial Microforge.
MF200 SPECIFICATIONS
MF200-1 Complete Microforge System, incl. W30S Microscope (110 v) MF200-2 Complete Microforge System, incl. W30S Microscope (220 v) MF200-M1 MF 200 without microscope (110v) MF200-M2 MF 200 without microscope (220v) *Above MF200 microforges include 40X long working distance objective
OPTIONAL ACCESSORIES
500292
Optional 15× Eyepieces (pair) Note: No reticle available for 15× eyepieces
500329 25× Long-Working Distance Objective (fits most microscopes with a 160 mm Focal Length) 13142
Optional foot switch REPLACEMENT ACCESSORIES
MF200-H2 Replacement heating filament (large gauge)
MF200-H3 Replacement heating filament (medium gauge)
MF200-H4 Replacement heating filament (small gauge)
75090 Filament Adjustment Assembly for 22mm OD Objectives
75050
Replacement Micropipette Slide
75040
Replacement Filament Cable
100-240 VAC 50/60 Hz
FILAMENTS (3)
H2, H3, H4
FILAMENT ON
Pushbutton Controlled or Optional Foot Switch Controlled
FILAMENT ADJUSTMENT ASSEMBLY For 40× and 25× Long-Working Distance Objectives: mounts on objective
OBJECTIVE
40× Long-Working Distance (3 mm)
OPTIONAL
25× Long-Working Distance (5 mm)
EYEPIECE10× (pair)
RETICLE (10× eyepiece only)
1.25 µm/division (at 40×)
0-90º Angle at 5º/division
15× (pair)
OPTIONAL EYEPIECE
GLASS HOLDER
Mounts on Microscope Stage
DIMENSIONS: Control Unit
4 × 7 × 17⁄8 in. (10.2 × 17.8 × 4.8 cm)
SHIPPING WEIGHT
3 lb. (1.4 kg)
MICROSCOPE See W30S
16 lb. (7.3 kg)
SHIPPING WEIGHT
Microforge Feature COMPARISON
MF200DMF1000
BEVELERS
AC POWER MODULE
W30S Microscope
✔
✔
40x Long Working Distance Objective
✔
✔
Analog Controller
✔
✔
Digital Controller —
✔
Pressurized Air Control—
✔
Microinjection Capability—
✔
Optional Foot Switch
✔
✔
Memory—
✔
Auto-sense of Filament Type—
✔
Digital Temperature Control—
✔
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
125
Microforging, Micropipette Calibration
and Microinjection — in a single device!
PULLERS, BEVELERS
The DMF1000 is a ‘state-of-the-art’ microprocessorcontrolled microforge offering unmatched
performance. Designed for fabrication of both small
patch clamp glass pipettes and larger injection
pipettes, the DMF1000 should find many uses in the
laboratory. The DMF1000 is based on a design similar
to that first used in WPI’s extremely popular microforge
model, the MF200. The extensive improvements
incorporated into the DMF1000 greatly increase its
versatility and performance, making it one of the most
powerful microforges on the market.
Digital Signal Processor (DSP)
Technology
The DMF1000 is powered by the latest digital signal
processor (DSP) technology. A digital timer is used
to precisely control the polish heating time. Ten
memories can be used to store settings of the heating
power and heating duration. All of the settings are
controlled and displayed digitally for better accuracy
and reproducibility. Two different operating modes
are provided: Manual and Auto. In the Manual mode,
the DSP will memorize the duration of the time that
is used to achieve a desired polishing. In Auto mode,
the heat will be applied for the duration of the timer
setting.
Filament Holder mounts directly to objective to provide precise
control of heating element position.
Unique Features of the DMF1000
System
The DMF1000 system includes a specially configured
WPI model W30S research grade compound
microscope equipped with a high quality metallurgic
40x long-working distance objective and a pair of
10x eyepieces. It is the most powerful long-working
distance objective currently available on any
commercial microforge. The long working distance
objective reduces the danger of damage to the
objective lens during the heating process.
Ease of use
The Heating Filament
Other benefits of the DMF1000 design include the
use of a Kohler illuminator and Abbe condenser, which
provide the reduced glare and sharper image contrast
necessary when polishing pipettes as small as half a
micron (0.5 µm) in diameter.
MICROFORGES,
Pressure Polishing
The DMF1000 incorporates a unique digital pneumatic
pressure feature that enables pressurized air to be
delivered through the pipette during fire polishing. In
the fabrication of patch pipettes, the pressurized air
can be used to blunt the taper at the pipette tip without changing the size of the tip opening. This reduces
electrical resistance of the tip, leading to lower noise
during patch-clamp recordings (Goodman & Lockery,
2000).
With a conventional microforge often the most difficult
and time-consuming part of using a high magnification
objective is being able to move both the heating
filament and the pipette into the same viewing area.
Finding and moving both the heating filament and the
pipette without collision can be a challenge. However,
this difficulty is eliminated with the DMF1000 because
the heating filament is directly attached to the
microscope’s objective. Hence it can be easily adjusted
to any position within the viewing area.
The low heat capacity and low thermal
coefficient of linear expansion of the filaments
are key design features of the DMF1000. The low
heat capacity of the filament allows it to reach fire-
polishing temperatures without excessive heat. This
permits the user to bring the pipette tip close to the
filament during polishing without fear of collapsing
the pipette tip. Low heat capacity eliminates the
need for an auxiliary air-cooling system. The
low coefficient of expansion characteristic of the
filament ensures minimal displacement of the
filament during heating. This feature eliminates
much of the guesswork out of tip placement in
relation to the filament.
Two different heating filaments are provided with
the DMF1000 to accommodate various applications.
The H5 filament is large gauge and can be reformed
into a “U” for fabrication of pipettes, air forming of
patch pipettes and other applications. The H4 is a
smaller gauge filament and is ideal for polishing patch
clamp pipettes.
The Pipette and Microscope Stage
The pipette rests on a specially designed holder that
sits on top of the microscope stage. The position of the
pipette, relative to the heating filament, is controlled
by the (X, Y, Z) adjustment of the stage. This unique
design makes locating and polishing the pipette
extremely easy. The stage of the microscope has a
high quality rail that gives precise, smooth and stable
control of the pipettes movement. This configuration
also eliminates the need and expense of an additional
micromanipulator to control pipette movement.
Fire
Polishing
126
Large Tip
Sharpening
(contact
stretching)
Tip Sealing
Tip Reducing
(holding
pipettes)
Tip Bending
Carbon Fiber
Sealing in
Plastic Sensor
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Typical applications of the DMF1000
Polishing the Patch Pipettes
Microforging Holding Pipettes
A holding pipette with a large blunt tip and a small opening is used to hold a
floating cell in place prior to microinjection by applying suction to the rear of the
pipette. The procedure for making holding pipettes involves three steps: squaring off,
large bore flame polishing, and tip reducing. These steps are accomplished with a
larger heating filament.
Microforging Beveled Injection Pipettes
Occasionally, a beveled large bore pipette is not sharp enough to penetrate a cell
without damaging the area around the pipette. With the DMF1000 and the large
heating filament, a sharp point can be formed on the beveled tip to assist the
penetration of the cell. This process is referred to as contact stretching.
ProfessionalGrade
Microscope
The W30 professional-grade
microscope is a best-seller in
universities, medical schools,
and reseach laboratories.
Equipped for performance,
its features include titaniumfinished DIN or Semi-Plan
optics and a 30-year anti-fungal
coating. The W30 is the choice
for superior performance at a
great price.
W30S
W30ST
503513
503514
Binocular Microscope
Trinocular Microscope
21 mm 10X Eyepiece with 100/10 reticle
Replacement Lamp
Pipette Tip Calibration & Microinjection
The integrated digital pneumatic pressure system can be used to calibrate the
precise diameter (I.D.) of a micropipette tip, based on a technique described
previously (Hagag & Randolph 1990, Bowman & Ruknudin 1999). The pressure
system can also be used separately as a simple but highly accurate controller for
microinjection applications.
DMF1000 SPECIFICATIONS
AC POWER MODULE
TIMER RANGE (for heater & timer)
NUMBER OF MEMORYS
PRESSURE ADJUSTING RANGE
PRESSURE RESOLUTION
FILAMENTS
HEATER AND TIMER CONTROL
DIMENSIONS: Control Unit
SHIPPING WEIGHT
MICROSCOPE
SHIPPING WEIGHT
100-240 VAC 50/60 Hz
0.01 to 360 sec
10
0.5 – 60 PSI (3.5 – 414 kPa
0.1 PSI (0.7 kPa)
H4 — Small filament for working with 40× long working distance objective.
H5 — Large filament for working with 10× objective. Filament adjustment assembly provided for both objectives.
Auto or Manual via Pushbutton, TTL, or Optional Foot switch.
4 × 7 × 17⁄8 in. (10.2 × 17.8 × 4.8 cm)
4 lb. (1.8 kg)
See W30S, page 205
16 lb. (7.3 kg)
MICROFORGES, PULLERS,
It is well known that the proper fire polishing of patch pipettes is the single most
important factor for forming a stable giga-seal in patch clamp recording. This is
even more important than the type of glass capillary used. Difficulties often arise
in forming giga-seals because the polishing of patch pipettes using a conventional
low magnification microforge is inadequate. However, since the DMF1000 uses a
40X long-working distance objective, pipette polishing is much more accurately
controlled. Pipettes polished using the DMF1000 achieve excellent stable giga-seals
with a wide variety of cells. Both whole cell patch pipettes and single channel patch
pipettes can be conveniently polished with the DMF1000 to the highest quality and
reproducibility achievable with any microforge.
For the single-channel patch clamp pipettes the pipette needs to be pre-coated
with Sylgard 184 before polishing. For this procedure the user can follow a simple
and effective coating method described previously (Li, 1999)
W30S SPECIFICATIONS
OPTIONAL ACCESSORIES
500329
25x Long Working Distance Objective, 5 mm 0.50NA
500292
Optional 15x Eyepiece (pair)
13142
Optional foot switch
REPLACEMENT ACCESSORIES
800292
40x Long Working Distance Objective, 3 mm 0.25NA
503513
21 mm 10X Eyepiece with 100/10 reticle
DMF1000-H5 Replacement heating filament (large gauge)
MF200-H4Replacement heating filament (small gauge)
75050
Replacement Micropipette Slide
75040
Replacement Filament Cable
Binocular (Seidentopf)
Inclined 30°, rotates 360°
Dual diopter adjustment, Interpupillary distance range 55-75mm
10X/18 wide field eyepieces
NOSEPIECE
Quadruple forward-facing nosepiece
OBJECTIVES
DIN Plan, anti-fungal
4X, 10X, 40X, 100XR (oil)
Parfocal, parcentric, color-coded
STAGE
Mechanical stage (140mm x 140mm)
Coaxial drive controls
XY Movement: 73mm x 43mm
FOCUS
Coarse adjustment: range of 30mm
Fine adjustment: graduation of 2μm
Tension control knob
ILLUMINATION
Moveable Abbe condenser, NA 1.25,
Iris diaphragm
Variable halogen light source (12V/20W bulb)
110V/220V switchable electronics
DIMENSIONS AND WEIGHT 15” (38cm) x 9” (23cm) x 7” (17.8cm) (h x l x w)
14 lbs. (6.4kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
127
BEVELERS
DMF1000-1 Complete Microforge, incl W30S Microscope (110 v)
DMF1000-2 Complete Microforge, incl W30S Microscope (220 v)
DMF1000-M1 Microforge without microscope (110v) DMF1000-M2 Microforge without microscope (220v) *Above DMF1000 microforges include 40X long working distance objective
HEAD
MICROFORGES,
PULLERS, BEVELERS
Glass
Micropipette
Beveler for
submicron
tips
Easier cell impalement results in
less damage and longer cell life
An optically-flat mirrored glass disk, wetted with an abrasive slurry, spins
at 60 rpm (120 V), producing sharply beveled tips on fluid-filled glass
microelectrodes of one micron or smaller. This eases cell impalement
and improves the electrode's linearity. The microelectrode’s resistance
can be monitored during beveling with WPI’s Ωmega-Tip-Z™ megohm
meter (see page 81). The beveler is permanently mounted on a precision
magnetic plate that gives stable support for the optional 1350M
Micropositioner shown. Start-up kit includes 0.05 µm alumina abrasive
powder #3531, wick electrode, and wick support.
Model 1350M Micropositioner — This package (shown with beveler
above) includes WPI’s M3301R Manipulator and an M10 magnetic
stand. The stand-manipulator assembly mounts di­rect­ly onto the beveler
baseplate, allowing convenient positioning of elec­trodes onto the beveling
surface. Three axes of adjustment, including coarse and fine control in the
axis of the electrode.
SYS-1300MMicroelectrode Beveler & Start-Up Kit
(micropositioner not included)
Specify line voltage.
1300M SPECIFICATIONS
OPTIONAL ACCESSORIES
2478
Replacement Mirrored Disk
3531
Alumina Abrasive, 0.05 µm (5 g) fine
3551
Alumina Abrasive, 0.30 µm (5 g)
2479
Replacement “O” Ring SYS-OMEGAZ Ωmega-Tip-Z with Probe & Holder
1350M
Micropositioner (M3301R) and M10 Magnetic Stand
711P
Replacement Probe
5468
Adapter to connect metal microelectrodes to
probe, 2 mm socket to .031 in. receptacle Z-LITE
Z-Lite Fiber Optic Illuminator (115 V, 60 Hz, beige case)
Z-LITE-Z
Z-Lite Fiber Optic Illuminator (230 V, 50 Hz, black case)
500186
Bifurcated Light Guide with lenses
Z-LITE-186
Z-Lite Illuminator & Bifurcated Light Guide
Z-LITE-Z186 Z-Lite Illuminator & Bifurcated Light Guide
MES
Microelectrode Beveler System
BEVELING SURFACE
7.8 cm diameter, optically flat reflective glass
MAXIMUM BEVEL
ALUMINA ABRASIVE POWDER
RPM
MOTOR
POWER REQUIREMENTS
DIMENSIONS
Steel base plate
Overall height
Height of abrasive surface SHIPPING WEIGHT
0.5 µ , I.D.
0.05 µ size supplied (0.3 µ also available)
60 rpm at 120 V, 60 Hz; 50 rpm at 240 V, 50 Hz
AC synchronous
95-135 V or 220-240 V, 50/60 Hz
8.5 x 11 x 0.375 in. (22 x 28 x 1 cm)
4 in. (10 cm)
2.75 in. (7 cm) above base plate
20 lb (9.1 kg)
MES includes: 1300M Microelectrode Beveler; 1350M Micropositioner &
Magnetic Stand; OmegaZ; 5052 Steel Base Plate; 5468 Adapter; 3485
Ringstand Mount. Shipping Weight: 59 lb. (27 kg)
128
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
The only microbeveler system
with Guided Light!
Bevel micropipette tips larger than 1 micron,
for ap­pli­ca­tions such as microinjection
l Tool holder on microscope keeps
pipette in focus during beveling
l Includes stereo zoom microscope
PZMIII with up to 90x magnification
l Steel base provides solid support for
beveler and other magnetic stands
l Variable speed, reversible
l Pipette tip illuminated internally via
fiber optic illuminators
MBS
MicroBeveler System
Includes all components pictured above: 48000 MicroBeveler,
NOVA illuminator, fiber optic cable, PZMIII Stereo Zoom
Microscope with tilting base especially adapted for use with
MicroBeveler, two clear 20x eyepieces, one 20x eyepiece with
reticle, tool holder, and pipette holder FOIMPH.
SYS-48000MicroBeveler
optional
48015-03
Beveling Surface
3.5 inch diameter disk
48015-10
Abrasive Material
alumina, diamond
48015-30
48014-01
Speed of Rotation
100 to 5,400 rpm
48014-05
Motor
Reversible Direction
48014-10
Power Requirements
120 volts, 60 Hz or
48014-30
240 volts, 50 Hz, 20 VA 48025
to sup­plied power supply
Dimensions 15934
48300
Base Plate
8.7 × 11 × 0.4 in. (22 × 28 × 1 cm)
Overall Height
3 in. (8 cm)
48200
48000 SPECIFICATIONS
16 lbs. (7.6 kg.)
SHIPPING WEIGHT (MBS) 35 lbs. (16 Kg.)
Specify line voltage
accessories
Lapping Film, Alumina, 0.3 micron (50-pack)
Lapping Film, Alumina, 1 micron (50-pack)
Lapping Film, Alumina, 3 microns (50-pack)
Lapping Film, Diamond, 0.1 micron (3-pack)
Lapping Film, Diamond, 0.5 micron (3-pack)
Lapping Film, Diamond, 1 micron (3-pack)
Lapping Film, Diamond, 3 microns (3-pack)
Fiber Optic Cable for Pipette Illumination
Replacement Beveler Disk Plate
Tilt Base Assembly for PZMIII binocular head
PZM Tool Holder
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
BEVELERS
The advantage of WPI’s MicroBeveler over other types of solid-surface
bevelers is that the abrasive surface can be easily refreshed. Instead
of using a conventional solid abrasive disk, the MicroBeveler abrasive
surface is made of a high quality lapping film, widely used in the fiber
optics industry. When the surface is damaged or loaded up with glass
particles, the abrasive film can be easily replaced.
The solid polishing surface of WPI’s new MicroBeveler, turning at 5,400
rpm, provides sufficient cutting force for a very sharp tip in a very short
time. The cutting surface is very flat and turns very smoothly, ensuring an
undamaged tip.
SHIPPING WEIGHT (48000)
l Abrasive surface can be easily
replaced by several types of Diamond
and Alumina Lapping Film
MICROFORGES, PULLERS,
System now
includes PZMIII
stereo microscope
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
129
Programmable
Multipipette
Puller
●Double top panel structure.
●Automatic 4-Barrel or 7-Barrel
Pipette Puller.
●Exclusive Optical-Digital Ruler
Measurement.
●Computerized Real-Time Feedback
Heater Control.
PULLERS, BEVELERS
●Pneumatic Pulling Force and
Compact Size.
●Programmable and Savable
Sequences for Creation and
Reproduction.
●Manufacture Preset Pulling
Programs for 4 and 7-Barrel
PMP-107
The PMP-107 Programmable Multipipette
puller can pull a one to 7-barrel pipette with
easy push-button processing. Equipped with
a microcomputer, pneumatic pulling arm,
pneumatic rotator, optical-digital ruler and
specially designed clamp, the PMP-107
can automatically heat, twist and pull a
multibarrel pipette. There is no need for any
PMP-107 Programmable Multipipette Puller (110 V)
PMP-107Z Programmable Multipipette Puller (240 V)
manual rotation or any inconsistent timing
interrupt control. The whole pulling process is
programmable and under control of a preset
sequence. The PMP-107 is a new upgrade
model from the PMP-100 multipipette puller.
The rotation (twist) speed is adjustable.
Five-and Seven-Barrel Micropipette Blanks
High quality multi-barrel micropipette blanks for pressure injection
The P-5 and P-7 mi­cropi­pettes are designed for precise local ap­pli­ca­tion
of chem­i­cals in ex­tra­cel­lu­lar elec­tro­phys­i­o­log­i­cal studies. Drug ap­pli­ca­tion
may be by one of two meth­ods:
1. microiontophoretic ejec­tion of charged sub­stanc­es, or
2. pressure ejection.
MICROFORGES,
82
mm
P-5-10
P-7-10
PI-305A
MBMPH5-7
(
")
3⁄
1 4
P-5 and P-7 pipette blanks exhibit levels of quality and reproducibility impossible to match in hand­made pipettes. They are
compatible with most vertical pipette pullers that accommodate
3.00 mm diameter glass. P-5 and P-7 blanks have a solid ball
pulling tip and are fabricated from 3.00 mm Kwik-Fil tubing. All
barrels except the large central one have filaments.
5-Barrel Micropipette Blank (pkg of 10)
7-Barrel Micropipette Blank (pkg of 10)
Pressure Ejection Kit (1 kit)
Holder for P-5 & P-7 Pipettes
PI-305A Pressure Ejection Kit
contains 20 plas­tic fittings, an
in­sertion tool, five 5-ft lengths
of col­ored tubing, a five-valve
manifold cou­pling, six male luer
adapt­ers and one male/female
luered line. These Pressure Ejection
fit­tings con­nect the pres­sure
ejection tub­ing to the P-5 and P-7
pipette bar­rels quick­ly and se­cure­ly.
130
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
MICROFORGES, PULLERS,
r
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t
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S
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●● Store up to 30 programs in memory
●● Two factory programs installed
●● Tempered glass cover to reduce the effects of
●● Switchable power supply for any line voltage
90–240VAC ensures that line voltage fluctuations
don't affect reproducibility
This puller is a reasonably priced, compact, versatile and reliable
workhorse. The microprocessor, combined with the LCD display, makes
the PUL-1000 easy to use.
Construction
PUL-1000 Specifications
The cover of pulling chamber is made with tempered glass to minimize the
humidity effect on the reproducibility of pulled pipettes.
Heater Element . . . . . . . . . . . . . . . . . Platinum/Iridium
Pulling Force . . . . . . . . . . . . . . . . . . Solenoid, adjustable
Power Supply
Capillary OD Range . . . . . . . . . . . . . 1.0–1.5mm
Maximum Capillary Length . . . . . 170mm
Minimum Capillary Length . . . . . 55mm
Memory sets . . . . . . . . . . . . . . . . . . . 30
Power . . . . . . . . . . . . . . . . . . . . . . . . . . 90–240VAC, 50/60 Hz
Dimensions . . . . . . . . . . . . . . . . . . . . . 34 x 24 x 12cm
Shipping Weight . . . . . . . . . . . . . . . . 15 lb
Replacement Filaments . . . . . . . . . 2.5 mm Square Box Filament,
2.5 mm wide (# 13834)
PUL-1000 has a high quality switching power supply for use anywhere
in the world without worry about the line voltage differences. Pulling
reproducibility is unaffected by line voltage fluctuation. Heating voltage
can be controlled to within 0.1% accuracy even when line voltage
fluctuates from 90 to 240VAC.
PUL-1000 Micopipette Puller
13834
Replacement Filaments
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
131
BEVELERS
humidity on puller reproducibility
PUL-1000 is a microprocessor controlled horizontal puller for making
glass micropipettes or microelectrodes used in intracellular recording,
patch clamp studies, microperfusion or microinjection. The puller was
designed with tighter mechanical specifications and precision electronics
for complete control of the pulling process and accurate reproducibility.
It offers programmable sequences of up to four steps with heating, force,
movement and cooling time. This allows graduated cycles for applications
like patch clamp recording.
M ICROMAN I PU LATORS
Micromanipulators are used when precision work
is conducted under a microscope. A micropipette,
electrode or probe can be mounted on a
micromanipulator and move as little as a micron at
a time. This tool can be used for in vitro fertilization,
patch clamp experimentation, extracellular recording,
microinjection and any application requiring fine
mechanical placement (resolution). In addition to
micromanipulators, WPI offers tilt bases, piezo
translators and a variety of stands.
Micromanipulators can be broken out into three broad
categories: Manual, Manual/Motorized and Motorized.
Manual Micromanipulators
Manual/Motorized Micromanipulators
M3301 is a popular, high-quality
manual micromanipulator. Weighing
just 550g, the x-axis allows for
10µm resolution. Control knobs
for all three axes are aligned
in a single plane, protruding from the
back of the instrument for exceptional
control in tight spaces.
The KITE is the economy
version of the M3301. The
KITE
HS6 is a high precision
micromanipulator
built on a 12 pound steel plate. With
5µm resolution and 25mm of travel on
all three axes, it has 20 times greater resolution
than the M3301. The HS6 is a high end manual
micromanipulator.
The DC3001 is the popular,
DC3301
high-precision manual/motorized
micromanipulator. With 0.5µm
resolution, it can be used with an
optional joystick or an MS314
controller. (When sold with a
controller, it is a SYS-DC3314)
While it can be mounted on the
TBS, it is too heavy to mount
on the M3 tilt base. When performing intracellular
microinjection, the DC3001 can be used with the
MPM10 or MPM20 piezo translators.
Best r
Selle
M3301
HS6
MD4
MMJ
The mini micropositioners are also popular.
While they can be purchased separately
for one axis (MM1 or MM3) or three axes
(MM1-3 or MM3-3), they require an adapter
to mount them (MM1-A or MM3-A) and a
clamp to hold the pipette, electrode or probe
(MM1-C or MM3-C). The complete three axis
micropositioner with a magnetic stand are
also sold as units (MM1-ALL or MM3-ALL).
132
The HS6-3 must be used with
a high resolution microscope.
It includes manual controls
and a stepper motor that can
be controlled with a joystick,
keyboard, mouse or computer.
It has 10nm resolution and
can remember 999 positions
coordinates. It uses the same
controller and programmable software as the SM325 (MCL3).
HS6-3
Other manual micromanipulators include:
• MMJ–Includes a joystick for a variety of movements.
• MD4–Holds two electrodes on a common axis when you want
to record from two electrodes in a differential application or
when you want a reference electrode in close proximity to the
recording electrode.
• M325–Can be configured for left or right hand use. With
10µm resolution, it can carry loads up to 1kg. It can clamp
onto horizontal or vertical rods 10–12.7mm ∅.
M325
Best r
Selle
MM3-3
Motorized Micromanipulators
The SM325 is high
end of the WPI line of
micromanipulators. It boasts
of 40,000 steps/revolution
with 25nm/step and 25mm
travel. It tilts up to 90º and
can be controlled using a
joystick, keyboard, mouse or
computer using the MCL3
controller.
SM325
MM3-1
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Smallest micromanipulator features full
20 mm of piezo-movement in all axes!
●● Ultra-stable and precise electrode
positioning
●● Smooth movement with minimal
vibration
●● Ergonomic controller
can operate up to 14
manipulators
Features true electrode
angle adjustment
— 4th axis option
available soon.
SN-PZ-50 Specifications
SN-PZ-50 Micromanipulator
SN-PCZ Control unit
Positioning range
Minimum step size
Closed-loop control
Maximum speed
Maximum load
Table mounting
Electrode drive angle
Back-flip mechanism
Dimensions**
Weight**
Backlit display
Three rotary knobs with optical encoders
Can operate up to 14 micromanipulators*
Two memory positions for each micromanipulator
Dimensions
190 x 210 x 40 mm
Weight
510 g
AC Power
110-240 V; 50-60 Hz
20x20x20 mm3 (x-y-z)
30 nm
0.5 μm resolution and 3 μm repeatibility for absolute position
~3 mm/s
200 g*
magnet or bolt
0 - 35 deg
Combine control unit’s programmable memory
positions with back-flipping mechanism for
convenient electrode exchange.
*1-to-8 connector hubs available for connecting more than two
manipulators
32x40x80 mm
260 g
*Load balancing options available for loads exceeding 50 g
**Dimensions and weight without head-stage or electrode holder adapter; axis in initial positions
SN-PZ-50L Miniature Piezo Micromanipulator (left-hand version) and Controller
SN-PZ-50R Miniature Piezo Micromanipulator (right-hand version) and Controller
SN-PZC
Miniature Piezo Micromanipulator Controller
Specify line voltage
Battery-operated control unit with rotating knobs
and information display. Push buttons allow
easy speed adjustment and operation of memory
positions. Double-curved profile provides good
ergonomics and operation of rotating knobs
without moving hand.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
133
M ICROMAN I PU LATORS
●● Penetration mode with
high acceleration single
steps
●● Back-flip mechanism for
easy electrode exchange
●● Powered off in standby for
zero noise interference
Non-rotating Spindle Digital Micrometer Head
Build your own precision micro-positioning device
M ICROMAN I PU LATORS
502102 SPECIFICATIONS
The new non-rotating
spindle digital
micrometer head
allows you to create
your own micropositioning instrument.
With
micron-level accuracy, it gives higher precision
than a normal micromanipulator. Since
the spindle does not rotate as it advances,
instruments can be directly attached without
the need for a complicated decoupling device.
The digital display eliminates the need to squint
at the notational scale. Readings can be clearly
seen in either inches or millimeters. You can
read both absolute position and the increment
relative to a previously chosen point.
502102
Total Travel Distance
25 mm
Resolution
0.001 mm
Accuracy
± 0.003 mm
Spindle Diameter
∅ 8 mm
Mounting
∅ 12 mm x 10 mm
Total Length
166 mm
Measurement Mode
Absolute and
incremental
Digital Readout
mm or inch
Analog Readout
mm
Data OutputRS232
Environmental Protection IP54
Shipping Weight
0.51 kg (1.12 lb)
Non-Rotating Spindle Micrometer Head
Universal Manipulator Stand
Universal Micromanipulator Stands enable
scientists to mount their manual and
motorized micromanipulators at variable
angles and heights. A solid aluminum
platform with a grooved tower allows
the user to attach any micromanipulator
of any size or shape to the post for
infinite flexibility. Once mounted, the
micromanipulator can be set at any height
along the entire length (30 cm or 45
cm). The platform base comes configured
with industry standard pre-bored holes
(1/4-20 x 1” or M6 x 25 mm), allowing
direct mounting to any type vibrationfree table (for patch clamp recordings)
or optical bench (for laser and optical
measurements).
Using additional Rotation Clamps
(one included with stand), two or more
micromanipulators can be mounted on the
stand simultaneously in a space-saving
convenient manner. WPI’s Universal Stand
not only allows the user 360° flexibility
in manipulations but also promotes
independent angular transitions using a
single feather-light tensioner/adjustment
screw. This affordable stand is currently
the preferred choice in micromanipulator
stands — head and shoulders above setups using multiple magnetic-based stands
costing hundreds more when combined
with antiquated heavy steel base plates.
UMS SPECIFICATIONS
DIMENSIONS
Base plate
10.0 x 12.5 x 1.5 cm (LxWxH)
Stand
4.0 x 4.0 x 30 cm (LxWxH) (501622)
4.0 x 4.0 x 45 cm (LxWxH) (501623)
Mounting holesEnglish 1/4 20 x 1” (2 bolts supplied)
Matrix M6 x 25mm grid (2 bolts supplied)
SHIPPING WEIGHT
501622
9 lbs (4 kg)
501623
11 lbs (5 kg)
501622
Universal Micromanipulator Stand 30 cm high (includes one clamp)
501623Universal Micromanipular Stand 45 cm high (includes one clamp)
501624
Optional Rotation Clamp
VFPVibration-Free Platform
Vibration-Free Workstation — see page 159
Vibration-free Platform (VFP)
not included
134
The M3301, Kite and SM325
(but not M325) can be used
with these mounts.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
High speed
penetration and
precise control
Precise construction and special vibration
stabilizers ensure the MPM-10’s excellent
Piezo-Translator
Adapter MPH8
allows use of WPI
electrode holders!
DC3001 micromanipulator and
M-3 tilting base not included.
puncture characteristics. Lateral deviation from
the ideal axis of puncture (measured at the tip of
the electrode holder) is ± 5% of the step size.
Includes controller, piezo translator, electrode
holder, cables and mounting bracket. Shipping
weight: 6 lb (2.7 kg).
SYS-MPM10
Piezo Controller for DC3001 Motorized Micromanipulator
PM5Remote Controller for MPM-10
PM6Replacement Electrode Holder for MPM10
14104Record/Inject Electrode Holder for MPM10, MPM20
MPH8Electrode Holder Adapter for MPM10 & MPM20
M-3
80° Tilting Base 6mm x 1mm screw (Shipping Weight: 2 lb)
Specify line voltage
Adapter MPH8
allows use of WPI
electrode holders!
Piezo Translator
For use with M3301 and DC3001
micromanipulators
Especially recommended for use with the M3301 micromanipulator, the
MPM20 is a very efficient tool for intracellular injection. High penetration
speed and precise axial advance allows injection pipette to be brought
to its target position with tremendous accuracy. Lateral escape of the cell
is almost eliminated, and even tough membranes can be penetrated.
Independently selectable reverse speed setting can be used for fast
withdrawal, preventing adhesion of the injected cell to pipette tip. Mounts
directly onto DC3001 and M3301 micromanipulators. Use with DC3001
requires MS314 controller (for the micromanipulator).
The combination of the MPM20, a micromanipulator, and the PV820
PicoPump (see page 194) constitutes an extremely efficient system
for intracellular injection; cell penetration, injection and withdrawal are
executed automatically with the press of a button.
Shipping weight: 10 lb ( 4.5 kg ).
MPM20
SYS-MPM20 Piezo Translator
PM7Replacement Electrode Holder for MPM20
14106Footswitch for MPM20
Specify line voltage
Micromanipulator not included.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
135
M ICROMAN I PU LATORS
The piezo-ceramic element in WPI’s MPM10
provides high penetration speed over an
extremely short distance (0.5 to 10.0 µm).
Because the range of travel of a dedicated
piezomanipulator is much too limited for it to
be useful independently, it must be mounted
on a manipulator. The MPM-10 piezo translator
combined with a DC3001 motorized micromanipulator (available separately) provides a
single electronically controlled system.
When the piezo element is activated, the
MPM-10 axis carrying the micropipette shoots
forward at a rate which is set on the control
panel, then immediately returns (at a slower
speed) to its starting position. As soon as the
piezo element begins its reverse travel, the
motorized manipulator starts to travel forward.
The complimentary opposition of these two
travel sequences results in the micropipette tip
remaining in its advanced absolute position.
The three axes of the DC3001 are controlled
by six buttons. Pressing a button for less than
0.3 seconds activates one step, the size of
which (0.5 to 10.0 micron) is set on the Step
Size control. Pressing longer activates the
continuous mode, at the rate set on the Motor
Speed control. Pressing the button for the X
axis forward direction activates the piezo mode.
Advancement speed of the piezo element can
be separately adjusted from 1 to 100 mm/sec.
MPM10
Programmable High Precision
Motorized Micromanipulator
M ICROMAN I PU LATORS
suitable for patch clamp and IVF
WPI introduces a compact high
precision motorized micromanipulator (SM325). It features
low noise, high stability, a userfriendly software interface and
economy that are major concerns
in IVF and patch clamp research.
The SM325 is driven in all three
axes through high resolution stepping motors, which can achieve
40,000 steps per revolution
(25 nm/step) with completely
vibration-free motion. In a normal
lab environment, it can stay localized overnight without drifting.
The 25mm long range of travel
makes it unnecessary to have an
additional manual coarse adjustment.
Its compact construction makes
mounting onto the stage plate of
a microscope practical. The x-axis
can be tilted by 90° that allows
for a better positioning of the
injection tool. An additional tilting
fixture makes it possible to tilt
the tool holder for fast and easy
cleaning and exchange of the
injection tool.
The MCL3 controller features a
dynamic micro-step function that
makes very quick positioning
possible with maximum accuracy.
Motor control is achieved with
a linear output amplifier, which
also drastically reduces electronic
noise. Users can control the
micromanipulators by joystick,
keyboard, mouse or computer.
The user-friendly software
program can be enabled to
remember up to 999 position
coordinates from previous
procedures and can robotically
repeat this same positioning
sequence.
SM325-M
M3301EH Electrode Holder
included
MCL3-SM325
Universal Manipulator
Stand not included.
SM325
High Resolution 3-D Motorized Micromanipulator (SM325-M) & Controller (MCL3)
SM325-M High Resolution 3-D Motorized Micromanipulator
MCL3
Controller with Joystick and software for SM325-M
SM325 SPECIFICATIONS
CONTROL METHOD
Joystick, software, or both
TRAVEL DISTANCE
25 mm each axis
RESOLUTION
25 nm/step or 40,000 steps/rev
MAXIMUM SPEED
4 mm/second
POWER SUPPLY
120/240V, 50/60Hz
DIMENSIONS
SM325-M
5x7x5.5 in. (13x18x14 cm) (WxLxH)
9.8x9x3.7 in. (25x23x9.5 cm) (WxLxH)
MCL3
Optional Accessories
M3301EHReplacement Electrode Holder, straight, 14cm
15873
Angled Electrode Holder, 13 cm long
501622Universal Micromanipulator Stand, 30 cm high
501623Universal Micromanipulator Stand, 45 cm high
VFPVibration-Free Platform
SHIPPING WEIGHT
Vibration-Free Workstation — see page 159
SM325-M
6 lb. (2.7 kg)
11 lb. (5 kg)
136
MCL3
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Programmable Ultra High Precision
Motorized Micromanipulators
M3301EH Electrode
Holder included
The HS6-3 is supplied with manual controls and stepper motor drives in all
3 axes. The extremely solid construction eliminates the vibrations and drifts.
With the utmost precision and long travel distance in all three directions,
HS6-3 is the ideal tool for patch-clamp or electrophysiological applications. The tilting device is mounted
on the base plate serves as coarse height adjustment as well and the tool holder can be swiveled in all
directions.
The MCL3 controller features a dynamic micro-step function that makes very quick positioning possible with
maximum accuracy and free of vibration. Motor control is achieved with a linear output amplifier, which also
drastically reduces electronic noise. Users can control the micromanipulators by joystick, keyboard, mouse
or computer. The user-friendly software
HS6-3 SPECIFICATIONS
program can be enabled to remember up
Control Method: Joystick, software, or both
to 999 position coordinates from previous
Travel (X-Y-Z): 25 mm
procedure and can robotically repeat this
same positioning sequence.
Resolution: 10 nm/step
HS6-3
4.5 mm/sec.
Stability: 1 nm/hour at 24°C
Power Supply: 120/240 V, 50/60 Hz
Dimensions:
HS6-3: 6.1x9.7x9.9 in (15.5x24.6x25 cm) (WxLxH)
MCL3: 9.8x9x3.7 in (25x23x9.5 cm) (WxLxH)
Weight:
HS6-3: 13.2 lb. (6kg)
MCL3: 7.7 lb. (3.5kg)
M ICROMAN I PU LATORS
Maximum Speed: MCL3-HS6
HS6-3
High Resolution Motorized HS6 Micromanipulator and Controller includes HS6-3M and MCL3
HS6-3M High Resolution Motorized HS6 Micromanipulator MCL3
Controller with Joystick and software for HS6-3M OPTIONAL ACCESSORIES
M3301EHReplacement Electrode Holder, straight, 14cm
15873
Angled Electrode Holder, 13 cm long
501622Universal Micromanipulator Stand, 30 cm high
501623Universal Micromanipulator Stand, 45 cm high
VFPVibration-Free Platform
Vibration-Free Workstation — see page 159
DC3001
The DC3001 is the popular, high-precision manual/
motorized micromanipulator. With 0.5µm resolution,
it can be used with an optional joystick or an MS314
controller. (With the controller, it is sold as SYS-DC3314.) It can be mounted on the
TBS tilt base but is too heavy for the M-3 tilt base. When performing intracellular
microinjection, the DC3001 can be used with the MPM10 or MPM20 piezo translators.
Manual coarse controls use cross roller bearing slides. Vernier scales allow readings to
0.1 mm. All controls are closely grouped so adjustments can be made in any plane with
minimum effort. The DC3001 features DC motor drives and fine con­trol micrometers in
all three axes. Left- or right-handed versions of the DC3001 are supplied with a stan­dard
12 mm clamp. Standard accessories provided include one mi­cro­elec­trode holder and a
securing bolt and wrench.
The sophisticated MS314 Controller allows control of all three axes. Movements may be
continuous through the use of cross switches, or the controller can cause the DC3001
to step in defined increments. Steps as small as 0.5 µm are possible. A popular joystick
controller, STM3, allows control of the X, Y and Z axes.
If the MPM10 piezo system is used, it replaces the MS314 controller. This configuration
moves the motor forward as the piezo retracts, keeping the micropipette in the penetrated
cell. When using the MPM20, the MS314 is necessary as the DC3001 motor controller
because the MPM20 controls only the piezo element. The MS314 and MPM20 do not
interact with each other.
SYS-DC3314R
SYS-DC3314L
Manipulator (right-handed) & MS314 Controller
Manipulator (left-handed) & MS314 Controller
Specify line voltage.
System components also available separately:
DC3001R Motorized Ma­nip­u­la­tor, right-handed
DC3001L
Motorized Ma­nip­u­la­tor, left-handed
SYS-MS314Controller for DC3001
STM3
Joystick Controller for DC3001
OPTIONAL ACCESSORIES
TBS
Tilt Base with Screw Adjustment
PM5Remote controller for MS314 and MPM-10
5464
5-lb Weight for Tilting Base (shipping weight: 7 lb [3 kg])
M4C
Microscope Stage Adapter
M3301EHReplacement Electrode Holder (14 cm long)
15873
Angled Electrode Holder (13 cm long)
501607
Cable for MS314 and DC3001
TBS Tilt Base not
included
15873 Electrode
Holder not included
MS314 Controller
STM3 Controller (available separately)➡
DC3001 SPECIFICATIONS
Travel Range Resolution
Manual:X-axis 37 mm 0.1 mm
Y-axis 20 mm 0.1 mm
Z-axis 20 mm 0.1 mm
Travel Range Resolution Maximum Speed
Motorized: X-axis 10 mm Y-axis 10 mm 0.5 µm 0.5 µm 0.2 mm/sec
0.2 mm/sec
Z-axis 10 mm 0.5 µm 0.2 mm/sec
Shipping Weight:
DC3001: 3 lbs (1.4 kg)
1.8 lbs (0.9 kg)
MS314: STM3: 2.8 lbs (1.3 kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
137
M ICROMAN I PU LATORS
MM3-C
clamp
MM3-3
micropositioner
opens
0-7 mm
MM3-A
adapter
MM1-3
micropositioner
and MM1-C
clamp
opens
0-4 mm
MB2
magnetic
base
available
separately
MM1-A
adapter
(see page
156)
138
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
MM1-C
clamp
MM3-3
MM1-A
adapter
Mini Micropositioner
(MM1 and MM1-3)
Single stage measures only 5 × 11 × 26 mm with 3 mm travel. Provides
precise and smooth motion with no backlash, positive spring loaded carriage,
straight within 1 micron and less than 1 micron maximum wobble. Features
fine 80 TPI screw adjustment. 10 mm square mounting surface has a 3.9 mm
tapped center hole for transmission and/or mounting. Available in single X
(MM1), X-Y, and X-Y-Z (MM1-3) axis configurations.
Micropositioner (MM3 and MM3-3)
Single stage measures only 7 × 17 × 44 mm with 13 mm travel. Offers
precise and smooth motion with no backlash, positive spring-loaded carriage,
straight within 1.5 microns, and less than 1.5 microns maximum wobble.
Features fine 80 TPI screw adjustment. 13 mm square mounting surface has a
7 mm tapped center hole for transmission and/or mounting. Available in single
X (MM3), X-Y, X-Y-Z (MM3-3) axis configurations.
MINI-MICROPOSITIONER SPECIFICATIONS
MM3
MM1
MM1
MM1-3
MM1-A
MM1-C
MM3
MM3-3
MM3-A
MM3-C
MM3-ALL
MM1-ALL
MM1
MM1-3
MM3
AXIS
X
X-Y-Z
X
X-Y-Z
STRAIGHT LINE
ACCURACY
Within 1 micron
over 3 mm travel
Within 1 micron
over 3 mm travel
Within 1.5 micron
over 13 mm travel
Within 1.5 micron
over 13 mm travel
3.9 mm tapped
3.9 mm tapped
hole, 8-32 thread hole, 8-32 thread
7 mm tapped hole,
5/16-16 thread
7 mm tapped hole,
5/16-16 thread
CLEAR
APERTURE
MM3-3
LOAD CAPACITY
255 g Normal
255 g Normal
340 g Normal
340 g Normal
FINISH
Black Anodized
Black Anodized
Black Anodized
Black Anodized
WEIGHT
3 grams/axis
12 grams/axis
14 grams/axis
48 grams/axis
TYPE
Fine Screw
Fine Screw
Fine Screw
Fine Screw
TRAVEL
3 mm
3 mm
13 mm
13 mm
Mini Micropositioner, one axis, 3 mm travel
Mini Micropositioner, three axes, 3 mm travel
Mounting Adapter for MM1 and MM1-3
Clamp for MM1 and MM1-3
Micropositioner, one axis, 13 mm travel
Micropositioner, three axes, 13 mm travel
Mounting Adapter for MM3 and MM3-3
Clamp for MM3 and MM3-3
Complete 3-Axis Micropositioner & Magnetic Stand
Complete 3-Axis Mini Micropositioner & Magnetic Stand
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
139
M ICROMAN I PU LATORS
MM1-3
KITE
M3301 Manual
Micromanipulator
M ICROMAN I PU LATORS
The world’s most widely used micromanipulator
Weighing just 550 grams and employing a slim space-saving design, this
well-built micro­ma­n­ip­u­la­tor outsells all oth­ers world­wide for high precision
experiments where mag­ni­fi­ca­tion is in the range of up to 250×. Its design
allows units to stand tightly grouped — since all control knobs project to the
rear. And because con­trol knobs are clustered with­in an 8 cm area in a sin­
gle vertical plane, res­o­lu­tion is quick — the hand works blindly while the eye
monitors the microscopic im­age. Vernier scales allow readings to 0.1 mm;
x-axis fine control allows readings to 10 microns.
The instrument employs rack-and-pinion drive, V-shaped guideways,
and cross roller bearings, so all movement is sure and repeatable, without
drift, sideplay, back­lash, or sticking. Contact parts are milled of hardened
steel for high performance and long life.
Left- or right-handed versions of the M3301 are supplied with a
standard 12 mm clamp (M2) and one microelectrode holder
(M3301EH).
KITE-R
15873
Electrode Holder
not included
Economy Manual Micromanipulator
Vernier scales allow readings to 0.1 mm. X-axis fine control allows
readings to 10 µm.
Left- or right-handed versions of the KITE mi­cro­ma­n­ip­u­la­tor are supplied
with a standard 12 mm clamp and electrode holder M3301EH.
KITE-R
Kite Manual Manipulator (right-handed)
KITE-L
Kite Manual Manipulator (left-handed)
KITE-M3-R Kite (right-handed) + Tilting Base Combo
KITE-M3-L Kite (left-handed) + Tilting Base Combo
Shown on
optional M-3
Tilting Base with
optional 5-lb
Weight #5464
KITE SPECIFICATIONS
TRAVEL RANGERESOLUTION
X-axis Fine
10 mm
0.01 mm
X-axis
35 mm
0.1 mm
Y-axis
20 mm
0.1 mm
Z-axis
20 mm
0.1 mm
SHIPPING WEIGHT
3 lbs (1.4 kg)
M3301R
M3301L
M3301-M3-R
M3301-M3-L
502105
Optional Accessories
M3301EH Re­place­ment Electrode Holder (14 cm long)
15873Optional An­gled Electrode Holder (13 cm long)
M-3
80° Tilting Base M6 x 1mm screw
5464
5-lb Weight for Tilting Base
Shipping weight: 7 lb (3 kg)
500475Ball Joint, 7 cm long, for ∅ 8 mm Holder
500476Ball Joint, 4 cm long, for ∅ 4 mm Holder
M4C
Microscope Stage Adapter
Also see magnetic stands.
140
Manual Manipulator, right-handed
Manual Manipulator, left-handed
Manual Manipulator (right handed) & Tilting Base Manual Manipulator (left handed) & Tilting Base Axis Adjustment Tool M3301 SPECIFICATIONS
TRAVEL RANGERESOLUTION
X-axis Fine
10 mm
0.01 mm
X-axis
37 mm
0.1 mm
Y-axis
20 mm
0.1 mm
Z-axis
25 mm
0.1 mm
SHIPPING WEIGHT
3 lbs (1.4 kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
s
’
e
:
r
e
e
l
g
H
an
ew
n
a
Ball-joint holder attachment
7 cm
7.9 mm diam.
500475Ball Joint, 7 cm long, for O.D. 5-9 mm Electrode Holder (shown)
500476Ball Joint, 4 cm long, for O.D. 2.8-4.5 mm Electrode Holder
80 mm
Interchangeable clamps allow ma­nip­u­la­tors to be
mounted on a variety of supports.
12 mm diameter
support arm
Quick, secure
mounting to any
microscope stage
(maximum opening:
20 mm).
M5 ∅ 10 mm clamp
M4C Microscope
Stage Adapter
— clamps onto
microscope stage
to provide a stable
support for any
manipulator with a
∅ 12 mm clamp.
M2 ∅12 mm clamp
M6 ∅ 1⁄2-in. clamp
M2
M5
M6
∅ 12 mm Clamp
∅ 10 mm Clamp
∅ 1/2-in. Clamp
M4C
Microscope Stage Adapter
At last — a tilt base you can operate with one hand!
Adjust tilt angle
easily by simply
turning knob of
thread­ed shaft.
Tilt angle may be
set at up to 35°.
High Precision,
High Stability
6.5 in.
or
lat ed.
ipu clud
n
a
M t in
no
Manipulator
mounting bracket
included.
3.5-pound steel base
provides additional
stability for your
mi­cro­ma­n­ip­u­la­tor.
Holes also allow permanent mount­ing to
your benchtop.
Two sets of mounting holes are pre­
drilled for WPI ma­nip­u­la­tors (M3301R
shown) but steel platform may be
drilled for mount­ing other devices.
TBS
Tighten locking
screw to lock
platform angle.
Tilt Base with Screw Adjustment
Shipping Weight 7 lb
5” x 6” footprint saves
space in your work area.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
141
M ICROMAN I PU LATORS
Mount a manipulator on
your microscope stage
High Resolution Manual Micromanipulators
Rock-solid —
superb resolution
M ICROMAN I PU LATORS
HS6 Micromanipulator
Engineered for stability, and built on a twelve-pound steel plate, this
instrument is chosen worldwide for high res­o­lu­tion micro-re­cord­ing.
such as patch clamping, and other research requiring sol­id, drift-free
per­for­mance. A superior tool in its own right, HS6 serves equal­ly
well as a base for other precision microdrives. HS6 can be bolted
directly to a lab fixture or vibration-free platform. Resolution
M3301EH
is extremely high — each gra­dua­tion on its large micrometer
electrode holder
barrels indicates just 5 micron movements. Rack and pinion
included
drive, V-shaped guide­ways, and cross roller bearings give sure,
re­peat­able move­ments without sideplay, slipping, or stick­ing. All
contact parts are milled of hard­ened steel. A flexible ball-joint assembly allows the electrode
to be positioned at any angle relative to the x, y, or z axis. The entire manipulator tilts
forward to 25 degrees allowing rapid coarse ad­just­ment of the elec­trode, and allowing cell
pen­e­tra­tion along the axis of any of the mi­crome­ters. Simple, precise, and durable, the HS6
will provide years of dependable per­for­mance.
HS6 SPECIFICATIONS
TRAVEL RANGERESOLUTION
X-axis
25 mm
5 µm
Y-axis
25 mm
5 µm
Z-axis
25 mm
5 µm
SHIPPING WEIGHT
25 lbs (11 kg)
DIMENSIONS
9.9 x 6.6 x 9.9 in. (H x W x L)
SYS-HS6Micromanipulator
M3301EH Replacement Electrode Holder (14 cm x ∅ 7.2 mm)
15873
Optional Angled Electrode Holder (13 cm long)
VFP
Vibration-Free Platform
Vibration-Free Workstation — see page 159
Joystick-Controlled
Micromanipulator
to
0°
Specially adapted for use with the Nanoliter Injector (page
186) for oo­cyte in­jec­tion and similar applications, this joystickcontrolled mi­cro­ma­n­ip­u­la­tor al­lows an easy “steer­ing” motion
that translates normal hand move­ment into smooth sub­mil­li­
me­ter shifts. Viewed mi­cro­scop­i­cal­ly, move­ment of the tooltip cor­re­sponds naturally to hand move­ment, so ac­cu­rate
M3301EH
resolution is intuitive and quick. All fine ad­just­ment can
electrode holder
be controlled by the joy­stick. Piv­ot­ing for­ward, back­
included
ward, or lat­er­ally gives pre­cise x-y ad­just­ment. For
add­ed con­ve­nience, a sep­a­rate coarse control lever
is also pro­vid­ed for quick rais­ing and low­er­ing. A stop screw—which is set once res­o­lu­
tion is achieved—eliminates re­fo­cus­ing and stream­lines re­pet­i­tive work by guiding the
tip to its pre­vi­ous fo­cus­sing plane. The stop screw also prevents the tool-tip from be­ing
broken during sudden low­er­ing and elim­i­nates down­ward drift—place­ment is stable
enough for even ex­tended use. Be­cause the probe holder tilts a full 90°, the tool-tip
pivots easily for precise positioning. Rack-and-pinion drive, V-shaped guide­ways, and
cross roller bear­ings elim­i­nate backlash, slip­ping, and sticking. All contact parts milled
from hardened steel for precise performance and long life.
°
90
s)
ntrol
JR
co
M M handed
t
h
(rig
Joystick travel: 0.35 mm to 3.5‑mm, de­pend­ing on reduction gear ratio setting
.(adjustable between 1:15 and 1:150).
MMJR
MMJL
Joystick Micromanipulator (Right-Handed)
Joystick Micromanipulator (Left-Handed)
MMJ SPECIFICATIONS
OPTIONAL ACCESSORIES
M3301EHRe­place­ment Electrode Holder (14 cm 5 ∅ 7.2 mm)
15873
Angled Electrode Holder (13 cm long)
M4C
Microscope Stage Adapter
500475Ball Joint, 7 cm long, for ∅ 8 mm Holder
500476Ball Joint, 4 cm long, for ∅ 4 mm Holder
TRAVEL RANGERESOLUTION
X-axis
37 mm
0.1 mm
Y-axis
20 mm
0.1 mm
Z-axis
25 mm
0.1mm
JOYSTICK (X,Y axis)
0.35~3.5 mm
SHIPPING WEIGHT
4 lbs (1.8 kg)
Also see magnetic stands.
142
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Micrometer Slide Micromanipulator
The M325 can be configured
from left- to right-handed
version in 60 seconds!
Toolholder rotates
around two axes, can
hold tools from ∅3.0 mm
to ∅12.7 mm.
Mounts onto horizontal
or vertical rods — any
diameter from 10 mm to
12.7 mm.
M325 SPECIFICATIONS
TRAVEL RANGERESOLUTION
X-axis
25 mm
10 µm
Y-axis
10 mm
10 µm
Z-axis
10 mm
10 µm
SHIPPING WEIGHT
4 lbs (1.8 kg)
M325
3-Axis Fine Controlled Manual Micromanipulator
OPTIONS AND ACCESSORIES
M3301EHReplacement Electrode Holder (14 cm long)
15873
Optional Angled Electrode Holder (13 cm long)
500475Ball Joint, 7 cm long, for ∅ 8 mm Holder
500476Ball Joint, 4 cm long, for ∅ 4 mm Holder
Also see magnetic stands.
Dual Tool-Holder Micromanipulator
A small and compact micromanipulator for manual manipulation in all three axes (x,
y and z), the MD4 is equipped with a mounting bracket for a second tool or elec­trode
holder which can be positioned in the x and y axes in­de­pen­dent of the manipulator
and may also be tilted and swiveled by two fine-adjust screws. Scales allow readings of
coarse adjustment with an ac­cu­ra­cy of 100 µm. Additional x-axis fine control is achieved
with a micrometer screw with a resolution of 10 µm. Supplied with one M3301EH
electrode holder and a 12 mm clamp for mounting on M10 Stand or other 12 mm
supports. May also be mounted on optional M-3 Tilting Base.
M3301EH
electrode holders
included
MD4L
(left-handed
controls)
Travel, standard electrode: x-axis, 37 mm (fine, 10 mm); y-axis, 20 mm; z‑axis, 25 mm.
Additional electrode: x-axis, 7 mm; y-axis, 10 mm.
MD4RDouble-Holder Micromanipulator (right)
MD4LDouble-Holder Micromanipulator (left)
MD4-M3-R Double-Holder Micromanipulator (right) + Tilting Base
MD4-M3-L Double-Holder Micromanipulator (left) + Tilting Base
OPTIONAL ACCESSORIES
M3301EHReplacement Electrode Holder (14 cm long)
15873
Optional Angled Electrode Holder (13 cm long)
M2Additional ∅ 12 mm Clamp
M-3
80° Tilting Base 6mm x 1mm screw
M4C
Microscope Stage Adapter
M5Additional ∅ 10 mm Clamp
M6Additional ∅ 1⁄2-in. Clamp 5464
5-lb Weight for Tilt­ing Base*
500475Ball Joint, 7 cm long, for ∅ 8 mm Holder
500476Ball Joint, 4 cm long, for ∅ 4 mm Holder
*Shipping weight: 8 lb (3.6 kg)
Also see magnetic stands.
M-3 Tilting Base not included
MD4 SPECIFICATIONS
TRAVEL RANGERESOLUTION
X-axis Fine
10 mm
10 µm
X-axis
37 mm
100 µm
Y-axis
20 mm
100 µm
Z-axis
25 mm
100 µm
SHIPPING WEIGHT
3 lbs (1.4 kg)
Also see Universal Manipulator Stands.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
143
M ICROMAN I PU LATORS
M3301EH
electrode holder
included
The M325 three-axis man­u­al mi­cro­ma­n­ip­u­la­tor is built of preci­sion
mi­crome­ter-ac­tu­at­ed lin­ear slides. Each slide is com­prised of a large
microme­ter head and a spring-return linear slide. The mi­cro­ma­n­ip­u­la­tor
has been carefully designed to min­i­mize wear in the moving components
to achieve a long op­er­a­tion­al life without the necessity for fre­quent main­
te­nance or ad­just­ment. The mi­crome­ter head is grad­u­at­ed in 10 mi­cron
steps which enable re­peat­able po­si­tion­ing to an ac­cu­ra­cy of ± 2 mi­crons.
A unique spring return mechanism is used to trans­mit move­ment of
the microme­ter spin­dle to the slide car­riage — eliminating back­lash, lost
motion and re­duc­ing thread wear. Each lin­ear slide utilizes ball bear­ings
which en­able the M325 to carry loads of up to 1 kg.
The toolholder can clamp onto tools with shaft di­am­e­ters of 3.0 mm
to 12.7 mm and allows rotation around two axes. This pro­vides a wide
range of options for in­cor­po­rat­ing the ma­nip­u­la­tor into your work­stations.
The M325 can also be con­fig­ured very eas­i­ly in left- or right-hand­ed
versions to allow several units to be positioned in close proximity.
A quick-release clamp allows easy mounting onto any rod from 10‑mm
to 12.7 mm diameter.
Magnetic
Holding Devices
12 mm ∅ x 25mm
M ICROMAN I PU LATORS
12 mm ∅
12 mm ∅
6-8 mm ∅
Useful as a probe holder,
the M11 is not suitable
for heavier items such as
micromanipulators.
M9
Mechanical clamp tightens three rotatable
joints simultaneously with one locking
knob. Arm adjusts without distortion.
Base exerts a magnetic force of 100 kilos
for greatest stability. Equipped with fine
adjustment for precise operations.
Magnetic Base:
50 ( w ) x 60 ( l ) x 55 ( h ) mm
( 2.2 x 2.4 x 2.2 in. )
Vertical Holding Power:
100 kgf ( 220 lb force )
Arms:
L1:
119 mm (4.7 in.)
L2:
106 mm (4.2 in.)
L3:
25 mm (0.98 in.)
Ø 12 mm (0.472 in.)
Clamp Hole:
none
Weight:
1.8 kg (4 lb)
M9
Magnetic Stand
M10
Magnetic Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Vertical Holding Power:
80 kgf ( 176 lb force )
Main Pole:
diameter: 14 mm (0.55 in.)
length: 178 mm (7 in.)
Sub Pole:
diameter: 12 mm (0.47 in.)
length: 165 mm (6.5 in.)
Clamp Hole:
Adjustable from 4.5 mm to 6.5 mm
Weight:
1.8 kg (4 lb)
M10
Powerful Ball
Joint Rare Earth
Magnet
Use in constructing your own holding
device for small parts/equipment
● Small but very powerful: holds 2 kilograms (~5 pounds)!
● Steel ball rotates freely 360° on a 180° axis
● M3 mounting screw on ball for attachment to equipment
144
M10L
Similar to M1 but with a 12 mm diameter
sub pole (fits 12 mm clamp supplied with
M3301, DC3001, MD4 and MMJ ma­nip­
u­la­tors).
M11
Same as M10 but equipped with a taller
(14-inch) vertical main pole.
Magnetic Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Bends freely for maximum flexibility. The
connecting arm twists and bends like a
snake. Lock the arm in position with a flick
of the controlling lever.
Magnetic Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Vertical Holding Power:
80 kgf ( 176 lb force )
Vertical Holding Power:
80 kgf ( 176 lb force )
Main Pole:
diameter: 14 mm (0.55 in.)
length: 356 mm (14 in.)
Main Pole:
diameter: 16 mm (0.63 in.)
length: 315 mm (12.4 in.)
Sub Pole:
diameter: 12 mm (0.47 in.)
length: 165 mm (6.5 in.)
Sub Pole:
none
Clamp Hole:
Adjustable from 4.5 mm to 6.5 mm
Clamp Hole:
Adjustable from 6 mm to 8 mm
Weight:
1.8 kg (4 lb)
Weight:
1.4 kg (3 lb)
M10L Magnetic Stand
M11
Magnetic Stand
● Magnet base threaded (M3) for mounting onto
a base or equipment
This novel magnetic ball joint has
phenomenal holding power for up
to 2 kg of attached weight while
permitting the ball a full 360° rotation
on a 180° axis. You can freely orient
your equipment to an infinite number
of positions within this rotation. This
is made possible by the combination
of a steel ball (10 mm diameter) and a
powerful rare earth magnet contained in
the magnet cylinder (φ 10 x 20mm). Convenient M3
attachment sites are provided on
Magnetic Stand
both the ball (male) and the
magnet base (female).
For use with micromanip­
ulators for the positioning and
holding of optical instruments
including various lighting
sources and lasers, pipettes
and any small parts that would
benefit from the flexibility
offered by this new magnetic
ball joint.
500871
M3 x 12 mm
28 mm
M3 x 5 mm
10 mm
Magnetic Ball Joint
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
The base of each stand exerts a
powerful magnetic force that holds
it solidly on ferrous metal surfaces
— even vertically or upside-down
10 mm ∅
10 mm ∅
5-6.5 mm ∅
MB2
M8
M1
A ball joint at the base of the main post
allows 360° rotation, offering con­sid­er­able
versatility. The second arm adopts angles
up to 75°.
A precision base providing stable support
for such devices as electrodes and manipulators. Adjustable second arm adopts a
variety of angles.
Magnetic Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Vertical Holding Power:
80 kgf (176 lb force)
Vertical Holding Power:
80 kgf (176 lb force)
Main Pole:
diameter: 12 mm (0.47 in.)
length: 194 mm (7.6 in.)
Main Pole:
diameter: 12 mm (0.47 in.)
length: 176 mm (6.9 in.)
Sub Pole:
diameter: 10 mm (0.39 in.)
length: 165 mm (6.5 in.)
Sub Pole:
diameter: 10 mm (0.39 in.)
length: 165 mm (6.5 in.)
Clamp Hole:
Adjustable from 5 to 6.5 mm
Clamp Hole:
Adjustable from 4.5 mm to 6.5­mm
Clamp Hole:
diameter: 4.5 mm and 6.5 mm
Weight:
0.38 kg (0.83 Ib)
Weight:
1.8 kg (4 lb)
Weight:
1.8 kg (4 lb)
MB2 Compact Magnetic Stand
M8
M1
Mechanical clamping type tightens three
joints simultaneously just by on-tough
operation. Arm is freely adjustable without
distortion. Equipped with fine adjuster
and medium size magnet for stabilizing
the base. Suitable for performing precision
operation.
Magnetic Base:
30 ( w ) x 30 ( l ) x 30 ( h ) mm
( 1.2 x 1.2 x 1.2 in. )
Vertical Holding Power:
17 kgf (37 Ib force)
Arm:
L1: 46 mm (1.8 in.)
L2: 46 mm (1.8 in.)
L3: 39 mm (1.5 in.)
Magnetic Stand
M1L
Same base and support arm as M1, but
equipped with a longer (14-inch) vertical
post.
Base:
50 ( w ) x 58 ( l ) x 55 ( h ) mm
( 2.0 x 2.3 x 2.2 in. )
Vertical Holding Power:
80 kgf (176 lb force)
Main Pole:
diameter: 12 mm (0.47 in.)
length: 356 mm (14 in.)
Sub Pole:
diameter: 10 mm (0.39 in.)
length: 165 mm (6.5 in.)
Clamp Hole:
diameter: 4.5 mm and 6.5 mm
Weight:
1.8 kg (4 lb)
M1L
Magnetic Stand
Magnetic Stand
Mounting your Micromanipulator
Three of the stands above — M1, M1L, and M8
— have 10 mm diameter mounting rods. The
standard mount on several WPI manipulators
(DC3001, KITE, M3301, MMJ, and MD4)
accommodates a 12 mm rod. In order to use
one of these three stands, you will need to
replace the manipulator's standard 12 mm
mounting clamp with the optional M5 clamp.
M5
∅ 10 mm Clamp
M5 — ∅ 10 mm clamp
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
145
M ICROMAN I PU LATORS
10 mm ∅
M ICROMAN I PU LATORS
Magnetic
Holding Devices
M7
Square Base
Round Base
An ideal accessory for optical tables and
vibration-free platform. Reduces experimental set-up time by allowing free positioning and instant clamp down of optical
components. Switchable ON/OFF magnetic
circuit permits fine adjustment and precise
positioning.
● Easy ON/OFF operation using lever
● Thin and powerful magnetic force
● Generous array of tap holes
Holding Power:
20 kgf (44 Ib force)
Dimension:
75 (OD) x 20 (h) mm
2.9 (OD) x 0.8 (h) in.
Mounting Hole:
4-M4 x 0.7, depth 6mm *
M8 x 1, depth 6mm
Span 35mm
Weight:
0.7 kg (1.5 Ib)
501651
503568
Magnetic Base, 75mm diameter
Magnetic Base, 50mm diameter
* Posts with M4-threads not available
from WPI. See posts with M8 threads on
page 142.
An ideal accessory for optical tables and
vibration-free platform. Reduces experimental set-up time by allowing free positioning and instant clamp down of optical
components. Switchable ON/OFF magnetic
circuit permits fine adjustment and precise
positioning.
● Easy ON/OFF operation using lever
● Thin and powerful magnetic force
● Generous array of tap holes
Holding Power:
20 kgf (44 Ib force)
Dimension:
65 (w) x 65 (l) x 20 (h) mm
2.6 (w) x 2.6 (l) x 0.8 (h) in.
Mounting Hole:
8-M4 x 0.7, depth 6mm *
M8 x 1, depth 6mm
Span 25mm
Weight:
0.6 kg (1.3 Ib)
501653 Magnetic Base, 65x65mm
503569 Magnetic Base, 45x45mm
503570 Magnetic Base, 90x90mm
503571 Magnetic Base, 120x120mm
146
MOBITY
MOBITYTM is a new magnetic clamping
system. With its ease of use, only one hand
is needed to operate the attractive power.
The MOBITYTM has a strong 88lbf pull,
yet weighs only 1.5 lbs. MOBITYTM meets
various applications with 4 tapped holes
on the top surface. Requires (1) 9V alkaline
battery (included).
Magnetic Base:
30 (w) x 35 (l) x 35 (h) mm
1.2 (w) x 1.4 (l) x 1.4 (h) in.
Vertical Holding Power:
20 kgf (44 Ib force)
Main Pole:
Diameter: 7mm (0.28 in.)
Length: 52mm (2 in.)
Clamp Hole:
Diameter: 6mm
Weight:
0.36 kg (0.8 Ib)
Holding Power:
40 kgf (88 Ib force)
Dimension:
55 (w) x 73 (l) x 50 (h) mm
2.2 (w) x 2.9 (l) x 2.0 (h) in.
M7 Compact Magnetic Stand Mounting Hole:
3-M4, depth 20mm *
M8, depth 15mm
Weight:
0.7 kg (1.5 Ib)
501652 MOBITY Magnetic
Clamping System
* Posts with M4-threads not available
from WPI. See posts with M8 threads on
page 142.
* Posts with M4-threads not available
from WPI. See posts with M8 threads
on page 142.
BASE PLATES: A magnetic stand
requires a steel mounting surface. WPI’s
steel base plates have plenty of mass to
give stability to your ex­per­i­men­tal setup.
Beveled edges make them easy to handle;
rubber feet hold them off the benchtop,
making them easier to grasp when moving;
and the special black coating provides a
durable protective finish.
A small holder ideal for use where space
is limited. Main post unscrews from base
which may then be used alone as a switchable magnetic holder.
81⁄2"x12" Steel Base
Plate #5052
12"x24" Steel Base
Plate #5479
ACCESSORIES
5052Steel base plate, 81⁄2 x 12 in. (10 lb)
5479Steel base plate, 12 x 24 x 3⁄8-in. (32 lb)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Vibration Isolation Platform
l Passive, mechanical and
maintenance free
l No air hoses or supporting
hardware required
l Isolates both horizontal and
vertical vibrations
This platform eliminates the transmission of
vibrations from a table top or counter to the
instrument that is mounted to it. This includes
microscopes, sample preparation tools and
hardness testers.
The platform is comprised of a black powdercoated, steel plate and three or four vibration
isolation bearings. It comes in two styles:
l Six degrees of freedom
l Compact design
Series 320 VIP — Rectangular shaped
platforms with three or four vibration isolation
bearings. Available in the following sizes: 12"
x 18", 16" x 16", 18" x 24" and 24" x 30",
supporting weights of 15-300 lb (7-136 kg).
All platforms are less than 2" high.
Series 3200 VIP — The platform design
matches the footprint of the isolated instrument.
(Microscopes by Carl Zeiss, Leica Microsystems,
Nikon and Olympus; Hardness testers by Wilson
Instruments)
M ICROMAN I PU LATORS
Call for more information and prices
for the configuration you require.
For more information, see
www.wpiinc.com/vfw
Vibration-Free Tables
All buildings vibrate — activities of people, machinery, heating and
ventilation systems, and nearby truck or rail traffic cause all types of
vibrations. These vibrations, though, acceptable to occupants, cannot
be tolerated by equipment used in patch clamping, cell injection,
analytical balances, and optical microscopes. The short-term effects of
such vibrations include inconsistant and unreliable performance. The
long-term effects are excessive wear, maintenance, and fatigue failures.
In order to protect sensitive instruments and equipment from faulty
operation or failure this vibration must be significantly reduced. This
can be efficiently accomplished by using Vibration-Free Platform and
Vibration-Free Workstation.
Vibration-Free Workstation
●
●
●
●
●
●
Vertical and horizontal vibration isolation
High performance Active-Air Suspension
Automatic leveling
VibraDamped Steel
Class 100 Cleanroom compatible
Leveling feet
Additional tabletop sizes and finishes
are available, as well as optional accessories such as side rails and casters.
Call for more information and prices for the
configuration you require.
For more information, see
www.wpiinc.com/vfw
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
147
Precision Stereo ZOOM Microscope
● Modular, two parallel
beam design
All PZ
M
micro IV and PZ M
scope
TIV
s co
10x ey
epiece me with
s, buil
1x au
t-in
xili
light r ar y lens, a
nd
ing ad
apter.
● Planachromatic objectives,
no optical distortion
● High-contrast imaging,
ideal for observing
transparent, low-contrast
objects
USBCAM50 digital
camera included with
version shown, also
available separately
● Large zoom range: 0.62x
to 5.0x
● Long working distance
Computer
and display
not included
● Step and continuous zoom
● 5-year warranty
The fourth generation of WPI’s precision
stereo zoom microscopes uses modular, two
parallel beam path design and high quality
optical system. The advanced optical design
with planachromatic objectives provides sharp
and distortion-free contrast image throughout
the entire zoom range and comes with an
impressive 5-year warranty.
The PZMIV is available in a binocular or
trinocular version. In addition, an extensive list
of optional accessories is available that makes
the PZMIV suitable for integrated optical and
biological research.
The microscope comes with a track stand,
standard 10x eyepieces (wide-field, distortionfree and high eye point), 1x planachromatic
distortion-free objective. See the Table on next
page for all optical options.
Fiber optic illuminator included
with systems below differs
from unit shown in photo.
Max. Working Range: 25 cm
MICROSCOPES, CAMERAS
● Large zoom ratio: 8:1
Shown:
PZMTIV-BS-LWD-DIG50
The trinocular version
is a true trinocular, with
continuous operation of
both eyepieces and photo
tube simultaneously. There
is no need to block the right
eyepiece to use the photoport.
PZMIV
PZMIV-BS
PZMTIV
PZMTIV-BS
PZMTIV-DIG50
PZMIV
148
Precision Stereo Zoom Binocular Microscope (Model IV), on Track Stand
PZMIV Microscope on Boom Stand (tubular) Precision Stereo Zoom Trinocular Microscope (Model IV), on Track Stand
PZMTIV Microscope on Boom Stand (tubular)
PZMTIV Microscope System, including PZMTIV, USBCAM50 USB Computer
Camera, 0.5× CCD Camera Coupler, Z-LITE Optical Illuminator, Bifurcated
Optical Fiber Light Guide
PZMTIV-BS-DIG50 PZMTIV Microscope System, including PZMTIV, USBCAM50 USB Computer
Camera, 0.5× CCD Camera Coupler, Z-LITE Optical Illuminator, Bifurcated
Optical Fiber Light Guide, Boom Stand
PZMTIV-BS-LWD-DIG50 PZMTIV Microscope System, including PZMTIV, USBCAM50 USB
Computer Camera, 0.5× CCD Camera Coupler, Z-LITE Optical Illuminator,
Bifurcated Optical Fiber Light Guide, Boom Stand, 0.5× Objective, 20×
Eyepieces for Long Working Distance Viewing
Please see web site for complete configurations
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
PZMIV
40 cm
21 in.
#502005 Ball Bearing Boom Stand
— Dual Arm Ball Bearing Boom Stand
for increased stability and smoother
horizontal movements.
15 in.
PZMTIV-BS
Base 11.25 x 10.25 in.
MICROSCOPES, CAMERAS
PZMIV & PZMTIV Eyepieces and Objectives
10x Eyepiece
16x Eyepiece
20x Eyepiece
25x Eyepiece
Objective
Mag
Field (mm)
(Video Field)
Mag
Field (mm)
(Video Field)
Mag
Field (mm)
(Video Field)
Mag
Field (mm)
(Video Field)
Working
Distance
0.32x
1.9x - 16x
106 -13.1
(49.8 - 6.1)
3.2x 25.6x
70.6 - 8.8
(49.8 - 6.1)
3.9x - 32x
55.4 - 6.9
(49.8 - 6.1)
5x - 40x
45.4 - 5.6
(49.8 - 6.1)
278 mm
0.5x
3.1x - 25x
67.7 - 8.4
(31.8 - 3.95)
5x - 40x
45.2 - 5.6
(31.8 - 3.95)
6.2x - 50x
35.5 - 4.4
(31.8 - 3.95)
7.8x - 62.8x
20 - 3.6
(31.8 - 3.95)
174 mm
0.63x
3.9x 31.5x
53.8 - 6.7
(25.3 - 3.15)
6.2x 50.4x
35.8 - 4.4
(25.3 - 3.15)
7.8x -63x
28.2 - 3.5
(25.3 - 3.15)
9.8x - 78.8x
23 -2.9
(25.3 - 3.15)
138 mm
1.0x (inc)
6.2x - 50x
33.9 - 4.2
(15.9 - 1.97)
9.9x - 90x
22.6 - 2.8
(15.9 - 1.97)
12.4x 100x
17.7 - 2.2
(15.9 - 1.97)
15.5x 125x
14.5 - 1.8
(15.9 - 1.97)
73 mm
The Video Field is based on a 1/2-inch CCD (8 mm diagonal) and a 0.5x camera adapter.
LED Ringlight
OPTIONAL ACCESSORIES
502000
PZMIV Binocular Body With 10X Eyepieces, 1× Objective, Eye guards
502001
PZMTIV Trinocular Body With 10X Eyepieces, 1× Objective, Eye guards
502004 Boom Stand (Heavy) W/O Focus Mount (requires 502009 Focus Mount for PZMIV)
502005 Ball Bearing Boom Stand (Heavy) W/O Focus Mount (requires 502009 Focus Mount for PZMIV)
502006 Boom Clamp Stand (Heavy) (requires 502009 Focus Mount for PZMIV)
504123
Extension for Heavy Clamp Stand
502007 Articulated Arm and Table Clamp w/o Focus Mount (requires 502009 Focus Mount for PZMIV)
502009 Universal Focus Mount for 76 mm PZMIV (Required for BS, AAC, BBS, and BCS) (5/8” pin)
502160
Lighted Fan Base, Fluorescent base, Tungsten Halogen beam
502010 10× Wide Field Eyepiece for PZMIV (pair)
502011 16× Wide Field Eyepiece for PZMIV (pair)
502012 20× Wide Field Eyepiece for PZMIV (pair)
502013 25× Wide Field Eyepiece for PZMIV (pair)
50026410× Eyepiece with Reticle (matches 10× eyepiece #502010)
50026620× Eyepiece with Reticle (matches 20× eyepiece #502012)
502015 Ring Light Adapter for PZMIV (For R-8-8-WPI01 Ring Light Guide)
502016 0.32×, Planachromatic Objective (Distortion-free) (278 mm WD)
502017 0.50×, Planachromatic Objective (Distortion-free) (174 mm WD)
502018 0.63×, Planachromatic Objective (Distortion-free) (138 mm WD)
502019 1.0×, Planachromatic Objective (Distortion-free) (73mm WD)
500261 0.35× CCD Camera Coupler, C-Mount (Use with USBCAM33)
500262 0.5× CCD Camera Coupler, C-Mount (Use with COLCAM, USBCAM50)
500028 1× CCD Camera Coupler, C-Mount (Use with COLCAM)
502163 Wall Mount Plate for Articulated Arm System
Z-LITE Z-Lite Fiber Optic Illuminator
500186 Bifurcated Light Guide with Lenses
R-8-8-WPI01 Ring Light Guide
Z-LITE-186 Z-LITE Fiber Optic Illuminator with Bifurcated Light Guide and Lenses
502167
Replacement Fluorescent lamp for Lighted Fan Base (502160)
502168Replacement Tungsten Halogen lamp for Lighted Fan Base (502160)
See Page 155
PZMIV SPECIFICATIONS
EYEPIECES
WFH 10×
AUXILIARY LENSES
1×
ZOOM RANGE
0.62× - 5×
TOTAL MAGNIFICATION
6.2× - 50×
ZOOM RATIO
8:1
FIELD OF VIEW
∅33.9- ∅4.2 mm
WORKING DISTANCE
80 mm
BINOCULAR TUBE
Inclined 45°
INTERPUPILARY DISTANCE
50 – 75 mm
DIOPTER ADJUSTMENT
± 5 Diopter
MICROSCOPE BODY
Rotatable 360°
OPTIONAL ACCESSORIES
Eyepieces
16×, 20×, 25×
Auxiliary lenses
0.32×, 0.5×, 0.63×
Total Magnification
1.9× - 125×
Field of view
∅106 - ∅1.8 mm
Working Distance
80 – 296 mm
SHIPPING WEIGHT
23 lb.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
149
Precision Stereo ZOOM Microscope
Shown:
PZMTIII-BS-DIG50
Includes Boom
Stand and USB
Digital Camera
MICROSCOPES, CAMERAS
Quality
and
Precision
to Improve
Your Vision
Computer
and display
not included
WPI’s third-generation stereomicroscope, PZMIII,
is an ideal tool for tissue dissection, cell injection,
specimen manipulation, electrode inspection, and
many other applications that require a magnified,
stereo viewing and ample working distance. It
offers the leading brand’s quality and performance
at an affordable price. Advanced optics provide
the sharpest image that can only be found among
the best of this class. It is superior to many
stereomicroscopes costing almost twice as much.
Zooming is achieved by a spring-loaded knob that
Fiber optic illuminator included with systems
below differs from unit shown in photo.
is smooth and effortless. The compact size and
light weight make it more stable and easily
manipulated on the boom stand. A specially
designed photo/video module is used in
the trinocular version of the microscope
(PZMTIII) for photo, video, or digital imaging.
PZMIII Precision Stereo Zoom Microscope (Model III), on Post Stand
PZMIII-BS
PZMIII Microscope on Boom Stand PZMIII-AAC
PZMIII Microscope on Articulated Arm with Table Clamp PZMTIII
Precision Stereo Zoom Trinocular Microscope (Model III)
PZMTIII-DIG50 PZMTIII Microscope System, including PZMTIII, USBCAM50 Computer
Camera, 0.5X CCD Camera Coupler, Z-LITE Optical Illuminator, Bifurcated
Optical Fiber Light Guide
PZMTIII-BS-DIG50 PZMTIII Microscope System, including PZMTIII, USBCAM50 Computer
Camera, 0.5X CCD Camera Coupler, Z-LITE Optical Illuminator, Bifurcated
Optical Fiber Light Guide, Boom Stand
PZMTIII-BS-LWD-DIG50 PZMTIII Microscope System, including PZMTIII, USBCAM50
Computer Camera, 0.5X CCD Camera Coupler, Z-LITE Optical Illuminator,
Bifurcated Optical Fiber Light Guide, 0.5× Objective, 20× Eyepieces for Long
Working Distance Viewing
PZMTIII-BS
PZMTIII Microscope on Boom Stand
PZMTIII-AAC
PZMTIII Microscope on Articulated Arm with Table Clamp
All PZMIII and PZMTIII microscopes come with 10x eyepieces and builtin 1x auxiliary lens.
PZMIII
150
In addition, an extensive list of optional
accessories is available that can make the
PZMIII suitable for almost any bio-research
applications requiring a stereomicroscope. See
next page for options.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
PZMIII
LED Ringlight
PZMIII-BS
See Page 155
#502007
Articulated Arm
#502006 Boom Clamp Stand —
Combines the stability of a boom
stand with the versatility of an
articulated arm.
Max. clamp
opening: 50 cm
PZMIII Eyepieces and Objectives
10× Eyepiece
Objective
Mag.
0.3×
2× - 13.5×
0.5×
3.4× - 22.5×
0.75×
5× - 33.8×
1.0×
6.7× - 45×
1.5×
10× - 67.5×
2.0×
13.4× - 90×
Field (mm)
(Video Field)*
114 - 17
(53.6 - 8)
69 - 10
(32.4 - 4.7)
45 - 7
(21.1 - 3.3)
34 - 5
(16 - 4.7)
23 - 3.4
(10.8 - 1.6)
12 - 2.5
(5.6 - 1.17)
15× Eyepiece
Mag.
3× - 20.3×
5× - 33.8×
7.5× - 50.6×
10× - 67.5×
15× 101.3×
20.1× 135×
20× Eyepiece
Field (mm)
(Video Field)*
84 -13
(53.6 - 8)
51 - 7
(32.4 - 4.7)
34 - 5
(21.1 - 3.3)
25 - 3.7
(16 - 4.7)
17 - 2.5
(10.8 - 1.6)
13 - 1.8
(5.6 - 1.17)
Mag.
4× -27×
6.7× - 45×
10× - 67.5×
13.4× - 90×
20.1×-135×
26.8×-180×
Field (mm)
(Video Field)*
69 - 10.3
(53.6 - 8)
42 - 6.2
(32.4 - 4.7)
28 - 4.2
(21.1 - 3.3)
21 - 3.1
(16 - 4.7)
14 - 2.1
(10.8 - 1.6)
10 - 1.5
(5.6 - 1.17)
25× Eyepiece
Mag.
5× - 33.8×
8.4× - 56.3×
12.6× - 84.4×
16.8×-112.5×
25.1×-168.8×
33.5× - 225×
Field (mm)
Working
(Video Field)* Distance (mm)
44.8 - 6.7
(53.6 - 8)
26.9 - 4.0
(32.4 - 4.7)
17.9 - 2.7
(21.1 - 3.3)
13.4 - 2.0
(16 - 4.7)
9.0 - 1.3
(10.8 - 1.6)
6.7 - 1.0
(5.6 - 1.17)
287 mm
177 mm
117 mm
100 mm
47 mm
26 mm
* The video field of view is based on a 1/2-inch (8 mm diagonal) CCD camera and a 0.5× camera adapter.
OPTIONAL ACCESSORIES
501352
PZMIII Binocular Body, pair of 10x eyepieces and eyeguards 13338 Ring Light Adapter NOT included
501353
Fan Post Stand with 76mm Focus Mount
501355
Universal Focus Mount, 76 mm ID for PZMIII Body
502004
Boom Stand (Heavy) without Focus Mount
502005
Ball Bearing Boom Stand (Heavy) without Focus Mount
504123
Post Extension for Heavy Boom Stand
502006
Boom Clamp Stand (Heavy) (requires 502009 Focus Mount for PZMIV)
502007
Articulated Arm with Table Clamp, without Focus Mount (50 cm clamp)
502163
Wall-Mount Plate, 6" × 6" (or 15.24 cm × 15.24 cm)
501369
Wide Field 10× Eyepieces (pair)
501370
Wide Field 15× Eyepieces (pair)
501371
Wide Field 20× Eyepieces (pair)
501372
Wide Field 25× Eyepieces (pair)
50412810× Eyepiece with Reticle (matches 10× eyepiece on PZMIII)
50412920× Eyepiece with Reticle (matches 20× eyepiece 501371)
5013730.3× Long Working Distance Objective Lens
5013750.5× Long Working Distance Objective Lens
5013760.75× Long Working Distance Objective Lens
5013771.5× Long Working Distance Objective Lens
5013782.0× Long Working Distance Objective Lens
501379
PZMTIII Trinocular Body, pair of 10× eyepieces and eye guards
13338 Ring Light Adapter NOT included
5013810.5× C-Mount CCD Camera Coupler
13338
Ring Light Adapter for PZMIII Series
(included with all microscope configurations on previous page)
503051
Manual Stage for PZMIII 503102
76mm Rectangular Base Post Stand for PZMIII
#503051 Manual Stage — Mounts in the
circular opening in the PZMIII base. XY travel
distance: 75 x 56 mm. Glass size: 116 x 96 mm.
Active diameter: 37.6 mm. Dimensions: 180 x 155
x 27 mm. Fits 503102 base only.
PZMIII SPECIFICATIONS
EYEPIECES
WFH 10×
ZOOM RANGE
0.67× - 4.5×
TOTAL MAGNIFICATION
6.77× - 45×
FIELD OF VIEW
∅ 34 MM - ∅ 5 MM
WORKING DISTANCE
100 mm
BINOCULAR TUBE
Inclined 45°
INTERPUPILLARY DISTANCE Adjustable 47-70 mm
DIOPTER ADJUSTMENT
±5 Diopter (both eyepiece tubes)
MICROSCOPE BODY
Rotatable 360°
AUXILIARY LENSES
Total magnification
2× - 225×
Biggest Field of View
∅ 110 mm
Working Distance
26-287 mm
SHIPPING WEIGHT
23 lb.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
151
MICROSCOPES, CAMERAS
#502163 WallMount Plate —
Mount the microscope
on the wall for
convenient storage
when space is tight.
Inverted Trinocular Microscope
Video camera
available separately
— see page 163
Condenser
Abbe condenser with
10× / 20× phase
Trinocular Head
Ideal for video/photography
Centering eyepiece for phase
objective
MICROSCOPES, CAMERAS
EW 10× /22 extra wide field
eyepieces
Stage
160×250 mm
Inserts: 35 mm
round, 50 mm round,
87×46 rectangular
Coaxial drive controls
Mechanical stage:
X-Y coaxial control;
120×78 mm range of
traverse
Nosepiece
Quintuple nosepiece
Objectives
Infinity Optical System
Objectives: Plan 4× and
40×, Plan Phase 10× and
20×
Illumination
6V/30W halogen bulb
Fixed Stage
Focus
Optics move during
focusing - excellent for
patch clamp and brain
slice recording
Coarse adjustment range +8 to -3 mm
Fine adjustment: 0.002 mm
Accessories Included
Green and neutral filters, dust cover.
Options
Multiple photo options
Weight
24 lb (10.9 kg)
152
INV-101
Trinocular Inverted Microscope
503510
30 mm 10X Eyepiece with 100/10 reticle
503520
Replacement lamp
503512
Deluxe Optical Cleaning Kit
All necessary tools are included for routine adjustments, alignments, and
assembly: 8 oz. Air Duster, 280 sheets lint-free lens paper, 1 oz. “no-residue”
lens cleaning fluid, 100 cotton-tipped applicators, 9x9 microfiber lens cloth,
soft-bristled dusting brush, micro-glide gear lubricant, allen wrenches,
double-sided friction collar wrench, precision screwdriver set, nylon carry case
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
3000-LED
Series
3000-LED SERIES Specifications
Superb Optics, Durable
and High Performance
Microscopes
Optical System
Infinity Optical System, f=180mm, Anti-Mold
Viewing Head
Siedentopf type, inclined 30°; interpupillary distance adjustment
48-75mm
Eyepieces
HWF Plan 10× eyepiece, 20mm field of view with built-in diopter
adjustment; a pointer is standard in one eyepiece
Nosepiece
Rear facing quadruple
Objectives
Infinity Plan achromat 4× (N.A. 0.10), 10× (N.A. 0.25), 40×R* (N.A.
0.65), 100×R oil* (N.A. 1.25) are standard
Stage
Stage size 140mm × 132mm with X-Y movement range of
76mm × 50mm
Phase Contrast (OPTION)
Phase sliders for 10×/BF/40×
Condenser
N.A. 1.25 Abbe condenser
Illumination
3 watt LED with variable intensity control
Accessories
Dust cover, immersion oil and instruction manual, universal power
supply 110v-240v
Stand
Cast alloy aluminum; coaxial coarse and fine focusing controls.
Dimensions
15.25 × 7.75 × 15.4 in. (387 × 196 × 391mm)
Weight
16 lb (7.26 kg)
504221 Binocular, Infinity Plan achromat 4×, 10×, 40×R and 100×R oil objectives
504443 Trinocular, Infinity Plan achromat 4×, 10×, 40×R and 100×R oil objectives
504445 Binocular, Plan phase contrast 10× and 40×R objectives
504444 Trinocular, Plan phase contrast 10× and 40×R objectives
5044160.50× c-mount adapter for 1/2" sensors, adjustable focus
5044170.35× c-mount adapter for 1/3" sensors, adjustable focus
W30 Professional-Grade
Microscope
W30S SPECIFICATIONS
HEAD
Binocular (Seidentopf)
True Trinocular
Inclined 30°, rotates 360°
Dual diopter adjustment, Interpupillary distance range 55-75 mm
10×/18 wide field eyepieces
NOSEPIECE
Quadruple forward-facing nosepiece
OBJECTIVES
DIN Plan, anti-fungal
4×, 10×, 40×, 100×R (oil)
Parfocal, parcentric, color-coded
STAGE
Mechanical stage (140 mm × 140 mm)
Coaxial drive controls
XY Movement: 73 mm × 43 mm
The W30 professionalgrade microscope is a
best-seller in universities,
medical schools, and
reseach laboratories.
Equipped for performance,
its features include
titanium-finished DIN or
Semi-Plan optics and a 30year anti-fungal coating.
The W30 is the choice for
superior performance at a
great price.
FOCUS
Coarse adjustment: range of 30 mm
Fine adjustment: graduation of 2 μm
Tension control knob
ILLUMINATION
Moveable Abbe condenser, NA 1.25,
Iris diaphragm
Variable halogen light source (12V/20W bulb)
110V/220V switchable electronics
ACCESSORIES INCLUDED
Replacement 0.5 amp fuses, mirror attachment (for field use), blue and green filters, dust cover, immersion oil, spare bulb
DIMENSIONS AND WEIGHT
15” (38 cm) × 9” (23 cm) × 7” (17.8 cm)
14 lb (6.4 kg)
W30S
W30ST
503513
503514
Binocular Microscope
Trinocular Microscope
21 mm 10X Eyepiece with 100/10 reticle
Replacement Lamp
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
153
MICROSCOPES, CAMERAS
The ACCU-SCOPE® 3000-LED Microscope Series delivers outstanding
optical performance, value and resolution to meet the exacting standards
of life science professionals and students. With a newly designed infinity
plan optical system, a best-in-class 20mm field of view and a super-bright
3-watt LED illuminator, the 3000-LED Series provides high contrast images
with outstanding resolution, precision design and enhanced illumination.
Precision
SurgioScope
MICROSCOPES, CAMERAS
Features:
● Motorized focusing system,
allows hands-free operation
● Light weight, compact and
easy to maneuver­—weighs
only 70 lb.
● Dual bulbs prevent
illumination failure during
surgery
● Optional video adapter
● Improved optics 119 lp/mm
● Convenient handles
● New head tilting mechanism
● Five magnification steps
Ideal for small animal
surgery
WPI’s improved precision SurgioScope (now with five
magnification steps) is a portable high quality surgical
microscope offering outstanding image quality and
value. Incorporating an agile extension arm and
excellent working distance objectives, the SurgioScope
provides convenient movement and maneuverability
necessary for accurate positioning. These important
features, together with a high quality optical system,
provide sharp image contrast and enhanced large field
of vision. The SurgioScope comes fully equipped with
a foot-controlled motorized focusing system, normally
only found in more expensive surgical microscopes.
A unique dual lamp housing enables safe and rapid
changing of the lamp during an operation, without
the need to power down. The optional video port on
the “T” version permits operational procedures to be
monitored or recorded simultaneously using a video
recorder and a COLCAM video camera or digital stills
with USBCAM50.
PSMB5N
PSMT5N
SURGIOSCOPE Specifications
Specify 89 cm or 103 cm post
Specify line voltage
TOTAL MAGNIFICATION (F200).............................................. 3.2× — 25×
ADJUSTABLE DIOPTER.............................................................. ± 6 Diopter
ADJUSTABLE INTERPUPILLARY DISTANCE ........................ min. 50 mm — max. 70 mm
EYEPIECE....................................................................................... 12.5x
Objective
Focal
Distance
F100
#504284
100 mm
F200
(included)
200 mm
F250
#504285
250 mm
F300
#504286
F350
#504287
Working
Distance
Magnification
step
Visual Field of
view (mm)
Camera field
1/2" CCD (mm)
Camera field
1/3" CCD (mm)
2x, 3x, 5x, 8x,
12.5x
24, 15, 9.5, 6, 4
23, 14.5, 9.5,
6, 3.75
16.5, 10.5,
6.75, 4.25, 2.5
195 mm
3.8x, 6x, 10x,
16x, 25x
48, 30, 19,
12, 8
46, 29, 19,
12, 7.5
33, 21, 13.5,
8.5, 5
~245 mm
3x, 5x, 8x, 13x,
20x
60, 39, 25,
16, 10
58, 37, 23,
15, 9
42, 27, 17,
11, 7
300 mm
6x, 9x, 15x,
24x, 37x
72, 45, 28.5,
18, 12
69, 43.5, 28.5,
18, 11
49.5, 31.5, 20,
12.75, 7.5
350 mm
6x, 11x, 17x,
28x, 43x
84, 52.5, 33,
21, 14
80.5, 50.8, 33,
21, 13
57.8, 36.8,
23.6, 14.9, 8.8
For additional objectives and specifications, please go to WPI website­—www.wpiinc.com
FINE FOCUS ADJUSTMENT RANGE...................................... 30 mm
Working Height (Arm Movement Range Above Floor)
89 cm Post........................... Focus on specimens 34.5" (88 cm) to 51" (130 cm) above floor *
103 cm Post......................... Focus on specimens 40.5" (103 cm) to 57" (146 cm) above floor *
* Subtract Working Distance for height above specimen, 103 cm post recommended for F350 objective.
154
Binocular SurgioScope, F200 objective (Specify post height.)
Trinocular SurgioScope, beam splitter, std video adapter, F200 objective (Specify post height.)
OPTIONS AND ACCESSORIES
501636 1/2" CS-mount Adaptor (requires Beam Splitter 501637)
501637 Beam splitter
504284 F100 Objective
504285 F250 Objective
504286 F300 Objective
504287 F350 Objective
500162 Replacement lamp, 12V, 100 W RANGE OF MOTION
Maximum Stretch Radius of Arm..................................870 mm
Vertical Movement Range...................................700-1100 mm
ILLUMINATION
Spot = 42 mm
Dual lamp housing with quick-change spare and internal coaxial fiber optic cable.
HALOGEN-TUNGSTEN LAMP....12V, 100W, with cold reflection
OPTIONAL CAMERA .................... COLCAM, USBCAM50 (1⁄2" CCD) USBCAM33 (1⁄3" CCD)
POWER................................... 110V, 50-60 Hz, or 220V, 50-60 Hz
SHIPPING WEIGHT...........................................................94 lb (43 kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
High intensity
source for
fiber optic
illumination
Bifurcated Light
Guide 500186
available separately
Z-LITE Specifications
Lamp
150 W quartz halogen
Size
30.5×25×25 cm (12×10×10 in.)
Power
115 VAC, 50/60 Hz, 3 A
Weight
5.9 kg (13 lb)
Z-LITE
Z-Lite Fiber Optic Illuminator (115 V, 60 Hz, beige case)
Z-LITE-Z
Z-Lite Fiber Optic Illuminator (230 V, 50 Hz, black case)
Z-LITE-186
Z-Lite Illuminator & Bifurcated Light Guide
Z-LITE-Z186 Z-Lite Illuminator & Bifurcated Light Guide
LIGHT GUIDES AND ACCESSORIES
500186
Bifurcated Light Guide (with lenses)
R-8-8-WPI01 Ring Light Guide for PZM and PZMIII Series*
13338
Ring Light Adapter (48 mm ∅) for PZM, PZMII, PZMIII
502015
Ring Light Adapter for PZMIV
5475
Adapter for SMA-terminated Fiber Optic Cables
*Ring Light Guide requires adapter #13338 for use with PZM, PZMII and
PZMIII, included with each PZMIII and PZMIV microscope.
LED Ringlight
Light guide
available
separately
for PZMiii and PZMiv stereo microscopes
●● “White” light illumination — 72 LED bulbs
ID: 58 mm
Ring Light R-8-8-WPI01 can be
used with PZM Stereo Microscope for
shadow-free illumination.
●● Maximum opening 61 mm
18-in. (46 cm) flexible cable
●● Brightness adjustable
●● Ring light is divided into four areas and
each area is turned on and off separately
●● ESD safe
●● Power supply AC 90-264V, 50/60 Hz, US plug only
504134
LED Ringlight
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
155
MICROSCOPES, CAMERAS
The Z-LITE Fiber Optic Il­lu­mi­na­tor
provides reli­able, un­in­ter­rupt­ed highintensity light for micro­scopes and
fiber op­tic spectrometers. Z-LITE
allows a continuous range of subdued
or concentrated light­ing controlled by
a rotary dim­mer on the front panel.
Z-LITE may be used with a ring light
and single or bi­fur­cat­ed flex­i­ble fiber
bundles, en­abling the light beam to
be placed ex­act­ly where need­ed.
Forced air cool­ing prolongs lamp life.
Lamp color temper­a­ture is 3350°K.
An interlock switch automatically cuts
off power when front panel is opened
to replace bulb.
21" (53 cm)
MICROSCOPES, CAMERAS
Incubator on a Nikon
Eclipse TE-2000
Microsocpe. Incubators
for Zeiss and Olympus
microscopes are also
available, as well as
versions that accommodate
confocal modules. All
incubators are compatible
with all commercially
available cameras, light
sources, filter wheels,
motorized stages, and
motorized nosepieces.
Live Cell Microscopy
This Live Cell Microscope Incubator was extensively tested in laboratories.
When compared with other systems, it offers dramatic advantages. For
example, other incubators for live cell microscopy rely on passive, random
diffusion of heated air from a single source to maintain the desired
temperature setpoint.
With no hot air return vent, the heated air escapes from the system
through cracks at the microscope/incubator junction in an uncontrolled,
random fashion. These systems offer no temperature uniformity,
suffer from focus drift and often experience electrical and vibrational
interference from the heater. You will also notice dramatic temperature
drifts when the imaging environment is disturbed.
Features
●●
Unique, diffusion grid, combined with air input and return vents
provide an air flow pattern for consistent, even heating, with no hot
or cold spots in the chamber
●●
External heater that can be placed far enough from the system to
eliminate electrical and vibrational interference from the heater
●●
High degree of temperature precision and stability
156
UK: Tel: +44 (0)1462 424700 • [email protected]
●●
Minimal focal drift after equilibrium is achieved– Accuracy ±0.1ºC at
the sample itself, and 0.2ºC across the microscope stage (allowing for
uniform heating of multiwell dishes)
●●
Airflow pattern and temperature uniformity eliminate dramatic
changes in environmental temperature when the incubator door
opens
●●
Ergonomic design for ease of use– The focus and x/y stage controls
are outside of the incubator itself. Large doors allow easy access to
the specimen and small ones for cords, tubing, etc.
●●
●●
Precision, shielded temperature probe
Simple, one person setup of the system
Live Cell Microscopy System Components
This unique, acrylic Live Cell Imaging chamber, combined with an
Environmental Control Unit (ECU) and an AirTherm controller, ensured
precision control of your incubator environment.
The ECU comes in four varieties so you have all the control you require.
With the ECU-H5, you can control air flow and heating. In addition to air
flow and temperature control, the ECU-HC and ECU-HCP let you control the
CO2 level. One has an internal sensor, and the other has a remote sensor
that can be positioned inside the microscope chamber. Finally, the ECU-
World Precision Instruments
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Ordering an Environmental Chamber
Acrylic enclosures are essentially custom-built. When ordering a
system, you will need to provide the following information:
Microscope
Stage
Perfect Focus
Camera
Right Port
Analyzer
Tirf
White Light Tirf
Tilting Head
Filter Wheels:
Emission
Dual Lamphouse
Cells
35mm/60mm
Coverslips
Stage-Up
Left Port
Fluor Attachment
Binocular D Head
Excitation
Transmitted Light Shutter
Wells
Consistent Air Flow
HOC adds control of the O2 level, which is accomplished by displacing the
oxygen with nitrogen. The first three ECU units are capable of controlling
a simple, external heater, like the AirTherm Satelite (AirTherm-SAT) or a
microscope lens warmer. The AirTherm-SMT can monitor and control both
temperature and humidity inside the microscope chamber.
Fig. 1–Single air input and no
venting causes random air flow
in a traditional incubator.
Fig. 2–Hot and cold spots result
from inconsistent flow.
Fig. 3–A diffusion grid with air
input and exhaust vents yields
consistent air flow.
Fig. 4–Consistent air flow means
uniform heating.
IV-100SMT
Environmental Chamber and AirTherm-SMT. Incubation system with flow regulator, proprietary humidification
module, stage adapter, stage dish, optical grade glass. System utilizes premixed 5% CO2 gas cylinders.
IV-200ECU
Environmental Chamber with CO2 and heat regulated via ECU-HC and AirTherm-SAT. Incubation system,
proprietary humidification module, stage adapter, stage dish, optical grade glass. System requires 100% CO2 and
ambient air gas supply and mixes at a defined rate.
IV-300ECU
Environmental Chamber actively regulated CO2 and heat via ECU-HCP and AirTherm-SAT. Incubation system,
proprietary humidification module, stage adapter, stage dish, optical grade glass. System probe directly measures
CO2 concentration at the sample and adjusts the stream of incoming CO2 via feedback. System requires 100% CO2
and ambient air gas supply and mixes at a defined rate.
IV-200-OX
Environmental Chamber with O2, CO2 and heat via ECU-HOC and AirTherm-SAT. Incubation system, proprietary
humidification module, stage adapter, stage dish, optical grade glass. System requires 100% CO2 and ambient air
gas supply and mixes at a defined rate.
IV-CUST
Custom Design Fee — includes (but not limited to) Tilting Heads, Black Chamber, Confocals, Camera on Right, HMX
Lamphouse, and Manipulators.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
157
MICROSCOPES, CAMERAS
Air flow affects the temperature uniformity of incubators. The red arrows
on Fig. 1 and Fig. 3 indicate air flow. The Live Cell Microscope Incubator
uses a diffuser grid and proper venting to insure consistent air flow.
Traditional incubators with poor air flow suffer with hot and cold spots
in the incubator, as seen in thermal images (Fig. 2 and Fig. 4). Warmer
temperatures are indicated by red and cooler temperatures by blue.
Stagetop
Environmental
Control
Control temperature and CO2 in a
microscope stagetop environment
●● Four programmable digital control loops
MICROSCOPES, CAMERAS
Independent incubator base temperature PID
control with ±0.1ºC precision
Independent incubator lid temperature PID
control with ±0.1ºC precision
CO2 digital PID control with ±0.1% precision
Airflow digital PID control from 0–900 SCCM
●● USB-based remote control and data logging
●● Electronic flow meter
●● Programmable alarm for out of tolerance
condition on all four channels
●● Compact and lightweight
For short term or long term studies of living cell cultures under
a microscope or for time lapse video research, a microscope
stagetop incubator is essential.
Perfect for Live Cell Imaging, STEV (the stagetop environmental
control system) is a compact environmental case that houses your
culture wells and fits
on a microcope stage
inside the live cell
microscope incubator.
This system offers
precision control of
both temperature and
carbon dioxide, as well as remote control and data logging via a
USB connection. The system is flexible and easy to configure for a
variety of experimental conditions.
The system includes the Environmental Control Unit electronics
which use four programmable loops to control the temperatures
of the case and the lid, CO2 within the environmental case and
airflow within the incubator.
IV-ECU-H5
IV-ECU-HC
IV-ECU-HCP
IV-ECU-HOC
STEV
158
The base unit (STEV) with lid removed, showing the placement of the
temperature sensor in one of the wells of a 96-well plate.
Chamber, Controller, Heat, Digital flow control for bottle gas
Chamber, Controller with CO2 and Heat (using CO2 internal sensor)
Chamber, Controller with CO2 and Heat (CO2 probe sensor)
Chamber, Controller with CO2, O2 and Heat (using CO2 internal sensor)
Stagetop Environmental Chamber
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
CO2 Controllers
external CO2 sensor
The CO2-200 carbon dioxide controller has an internal CO2 sensor. It
controls the concentration of CO2 in a background gas from 0.5–15%.
This controller offers:
The CO2-300 carbon dioxide controller has an external CO2 sensor. It
controls the concentration of CO2 in a background gas from 0.5–15%.
This controller offers:
●●
●●
A background flow rate of 25–250mL/min
An RS-232 interface to a computer
Optional air pump (not included) for economical use (as opposed to
using bottle pressurized background gas)
●●
●●
●●
●●
In the future, multiple gas controllers of this variety can be daisy
chained
●●
In the future, multiple gas controllers of this variety can be daisy
chained
CO2-200
A background flow rate of 100–1000mL/min
Optional air pump (not included) for economical use (as opposed to
using bottle pressurized background gas)
CO2-300
CO2 Controller
CO2 Controller
CO2-200
CO2-300
Power
110/240V, 50/60Hz
110/240V, 50/60Hz
10–50°C (50–122°F)
Operating Temperature (ambient)
10–50°C (50–122°F)
Operating Humidity (ambient)
15–70% RH, non-condensing
15–70% RH, non-condensing
Warm up Time
20 minutes
20 minutes
Computer Interface
USB via external USB/RS232 converter
CO2 Flow Meter
6–60mL/min
6 – 60mL/min
Background Flow Meter
20/250mL/min
100–1000mL/min
Sensor
Non-dispersive infrared (NDIR), dual
beam, 20s response time
Non-dispersive infrared (NDIR), dual
beam, 20s response time
Sensor range
0–20% CO2
0–20% CO2
Control Range
0.5–15%
0.5–15%
Control Precision
0.1% CO2
0.1% CO2
Control Accuracy
0.1–3% of reading
0.1– 3% of reading
<2.5% reading/year
Drift
<2.5% reading/year
Dimensions
7.5x6.5x8” (190x155x210mm)
7.5x6.5x8” (190x155x210mm)
Shipping Weight
10 lb. (4.6kg)
10 lb. (4.6kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
159
MICROSCOPES, CAMERAS
internal CO2 sensor
Precision Heater Controller
MICROSCOPES, CAMERAS
Smart, electrically quiet air heater for live cell
imaging systems and custom incubators
Maintenance of the desired temperature set-point can now be achieved
through the use of a precision, shielded temperature probe coupled to a
peripheral Air-Therm heating unit.
Precision: Noise is a major concern in any experimental application.
WPI’s Air-Therm™ is electrically quiet as well as acoustically quiet). Since
the Air-Therm can be placed away from the incubator, any residual
electrical and vibrational interference from the heater is virtually
eliminated. Accuracy of ±0.1°C can be routinely maintained at the sample
site (using a fully programmable differential algorithm to automatically
adjust the temperature on the Air-Therm unit). Air-Therm maintains a
uniform temperature across the microscope stage (allowing for uniform
heating of multiwell dishes). These tight tolerances result in minimal focal
drift once sample/incubator equilibrium has been achieved.
Glass Bottom Dishes: For high numerical aperture (NA) live-cell
imaging, cells must be plated on glass (e.g., WPI’s FluoroDish Glass Bottom
Dishes, spotting 0.17 mm glass thickness) or standard cover slips. Since
cover slips inherit other problems associated with sterility and accidental
damage to expensive microscope condensers, WPI designed new and
improved glass bottom dishes (35 mm × 10 mm or 35 mm × 22 mm)
that are not cytotoxic. A special adhesive in conjunction with an advanced
dish design makes the FluoroDish the only non-cytotoxic glass dish in
the world. (See page 164.) This means that cells can actually be cultured
for 4 to 5 days in the same dish and then carry the complete cell culture
with media from the incubator directly into the (Air-Therm controlled)
recording chamber. This is a comfort level that hitherto was unattainable
by scientists and clinical researchers since cells will have to be transported
from the culture dishes on to cover slips just prior to imagery or attaching
patch electrodes.
Chamber: The most problematic aspect of any live cell imaging
procedure or patch clamp approach is the thermal drift of high
magnification lenses caused by temperature fluctuations in the
microscope's environment. It is necessary to constantly readjust the focus
during image acquisition due to this thermal-drift, introducing artifacts
during quantitative microscopy due to the photo-bleaching that occurs
during refocusing. The optimal solution to this problem is to use WPI’s AirTherm temperature controller connected to a Plexiglas or acrylic chamber
that encloses the entire imaging area (or at least the objectives and the
stage). Using such a chamber will result in highly stable temperature
control and minimal thermal drift.
AIRTHERMY-ATXAir-Therm Heater 120 V, U.S.
AIRTHERMZ-ATXAir-Therm Heater 240 V Specify line cord
2 pieces of 4.5 ft clear, coil-reinforced heater hose included with Air-Therm.
Specify Line (Mains) Voltage
OPTIONAL ACCESSORIES
15590
Clear hose, 2.5” diam., 4.5 ft
300276
Replacement platinum temperature probe
3491
5 ft (1.5m) probe extension cable
160
AIR-THERM SPECIFICATIONS
AIR FLOW RATE
20 to 50 CFM (0.55 to 1.4 cubic meter/M)
CONTROL TEMP. RANGE
Ambient to 60°C
TEMPERATURE RESOLUTION
0.1 °C
TEMPERATURE ACCURACY
0.2 °C
CONTROL MODES
(a) Auto (PID set manually, or Self-Tune)
(b) Manual tune
ANALOG OUTPUT FOR CHART RECORDER
0.5°C resolution
0-10V, 0 to 100 C (default, adjustable via controller)
HEATING VOLUME
More than 4 cu ft (85 liter), non-circulating
POWER (CE)
300 W, 95-135 V or 220-240 V, 50/60 Hz
DIMENSIONS
61⁄2 x 8 x 71⁄2 in. (15.5 x 21 x 19 cm)
SHIPPING WEIGHT
10 lb (4.5 kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Digital Microscope Cameras
l Ultra-Compact USB cameras with color CCD
l Hardware & Software Trigger
USBCAM133 with a 1/3-inch color CCD.
USBCAM152 with a 1/2-inch color CCD.
USBCAM202 with a 1/1.8-inch color CCD.
All three cameras are cased models with a rear mini-USB connector;
hardware and software triggering image capture, digital zoom and a
feature-rich user based menu setup and control. Sentech USB cameras
include a SDK, DirectX, Twain and Linux driver, as well as the Sentech
Viewing Software.
Digital Microscope Camera,
Digital Microscope Camera, Digital Microscope Camera, Replacement USB Cable, 3m (10 ft)
CONN 6-PIN (external camera triggering control)
USBCAM133
USBCAM152
USBCAM202
Image Sensor
1/3” Interline SXVGA color progressive CCD
1/2” Interline SXGA color progressive CCD
1/1.8” Interline UXGA color progressive CCD
Cell Size
3.75 (H) x 3.75 (V) µm
4.65 (H) x 4.65 (V) µm
4.40 (H) x 4.40 (V) µm
Scanning System
Progressive
Progressive
Progressive
Resolution
1280 (H) x 960 (V)
1360 (H) x 1024 (V)
1600 (H) x 1200 (V)
Min. Scene Illumination
11 Lux at F1.2
18 Lux at F1.2
7.7 Lux at F1.2
Electronic Shutter
Auto / Manual (software selectable)
Auto / Manual (software selectable)
Auto / Manual (software selectable)
Gain
Auto / Manual (software selectable)
Auto / Manual (software selectable)
Auto / Manual (software selectable)
Gamma
Manual (software selectable)
Manual (software selectable)
Manual (software selectable)
White Balance
Auto / Manual / One shot (software selectable)
Auto / Manual / One shot (software selectable)
Auto / Manual / One shot (software selectable)
Input / Output
USB 2.0 High Speed
USB 2.0 High Speed
USB 2.0 High Speed
Power
+5 Vdc through USB connector, < 300 mA
+5 Vdc through USB connector, < 420 mA
+5 Vdc through USB connector, < 450 mA
Dimensions
28 (W) x 28 (H) x 37 (D) mm (excluding connector)
28 (W) x 28 (H) x 42 (D) mm (excluding connector)
28 (W) x 28 (H) x 42 (D) mm (excluding connector)
Lens Mount
CS mount
C mount
C mount
Weight
Approximately 45g
Approximately 45g
Approximately 45g
Interface Connector
USB: mini-B USB connector IO signal: 6pin connector
(HR10A-7R-6PB or equivalent)
USB: mini-B USB connector IO signal: 6pin connector
(HR10A-7R-6PB or equivalent)
USB: mini-B USB connector IO signal: 6pin connector
(HR10A-7R-6PB or equivalent)
RoHS
RoHS Compliant
RoHS Compliant
RoHS Compliant
USBCAM33 / USBCAM50
Record images directly to your computer.
These digital microscope cameras
offer flexibility, with a range
of configurations for image
capture, a choice of mount
option (C or CS) and file
output alternatives.
Since both cameras
connect via the
USB port, installing
the image capture
software is simple. Either camera
can be used on WPI’s stereo microscopes
PZMTIV, PZMTIII, compound microscopes
W30ST and GPL-T and also the PSMT5 Surgical
Microscope. Choose from the one third-inch CCD with 1024×768
resolution and 30 frames per second (USBCAM33) or one half-inch CCD
with 1280×960 resolution and 15 frames per second (USBCAM50).
USBCAM33
USBCAM50
503536
These cameras include IC Imaging Control 3.0 software that features:
lReal-time video preview
lText and graphics can be drawn on a live video stream
lScroll and Zoom
lAcquisition of single frames
lCapture pause, for intermittent image capture
lTimestamps
Digital Microscope Camera, 1/3-in. CCD
Digital Microscope Camera, 1/2-in. CCD
Cable, USB Extension (male-female)
USBCAM33
USBCAM50
IMAGE SENSOR
1/3” Sony CCD, progressive scan
1/2" Sony CCD, progressive scan
MAX RESOLUTION
1024 x 768
1280 x 960
SIZE
4.65 µm x 4.65 µm
4.65 µm x 4.65 µm
SPEED (PC DEPENDENT)
30fps, 15fps, 7.5fps or 3.75fps
15fps, 7.5fps or 3.75fps
SENSITIVITY
0.5 lux @ 1/15 s
0.5 lux @ 1/7.5 s
EXPOSURE, SHUTTER
CONTROL, WHITE BALANCE
Automatic/Manual
Automatic/Manual
INTERFACE
USB 2.0 cable
USB 2.0 cable
SYSTEM REQUIREMENT
Windows Vista (32 & 64 bit) or
Windows 7 (32 & 64 bit)
Windows Vista (32 & 64 bit) or
Windows 7 (32 & 64 bit)
SOFTWARE
IC Imaging Control Software
IC Imaging Control Software
LENS MOUNT
C/CS-Mount
C/CS-Mount
CAMERA BODY
50.6 x 50.6 x 50 mm
50.6 x 50.6 x 50 mm
WEIGHT
265 g (9.5 oz)
265 g (9.5 oz)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
161
MICROSCOPES, CAMERAS
USBCAM133
USBCAM152
USBCAM202
504570
504574
USBCAM133
USBCAM152
USBCAM202
Color Video Cameras for Microscopy
COLCAM-HD SPECIFICATIONS
MICROSCOPES, CAMERAS
l 720p (1280x720)
HD resolution
l Low cost alternative to existing HD and
3 CCD cameras, with outstanding image
quality
Imager
1/3" Interline SXGA CCD: ICX445AQA
l 16:9 Aspect Ratio,
1:1, no scaling
HD Active Picture Element
1280 (H) x 720 (V)
Chip Size
6.26 (H) x 5.01 (V) mm
l SXGA progressive
scan 1/3” Color
ExView CCD Sensor
Cell Size
3.75 (H) x 3.75 (V) μm
Minimum Scene Illumination 5 Lux at F1.2 at 50% IRE; AGC ON and 1/60 second
shutter speed with IR Cut Filter
l DVI signal output
via HDMI cable
Resolution
600 TV Lines
Sync System
Internal
Video Output
l 60fps or 50fps
selectable via OSD
remote control
DVI 1.0 compliant; RGB, 1280 (H) x 720 (V); 60 Hz /
59.94 Hz / 50 Hz
ALC
Can be configured via the UART communication; w/
Optical Iris, Auto Electronic Shutter and AGC
Shutter Speed
Adjustable shutter speed via the UART
Communication; Up to Up to 1/100,000 seconds at
60Hz / 59.94 Hz or up to 1/83,947 seconds at 50Hz
Gain
AGC or Fixed gain, selectable via the UART
communication; Gain range: 0 to 18.3 dB
Gamma
1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.45, 0.3 or manual
gamma; Gamma selectable via the UART
communication
White Balance
Auto / Manual / Push-to-set; Selectable via the UART
communication
Power
12V power jack; 2.1mm DC barrel power jack plug
w/ center pin position
Dimensions
40 (W) x 40 (H) x 45.8 (D) mm
Optical Filter
IR cut filter included
Lens Mount
C/CS mount
Video Output
HDMI connector
Weight
Approximately 120g
RoHS
RoHS compliant
l Available in “C”
or “CS” Mount
COLCAM-HD
CCD-based camera outputs a true HD 720p, 16x9 image at 60fps. It
also features the capability to program 28 individual DSP profiles and
has a programmable on-camera push-button for manual control, initially
programmed to set White Balance (requires optional Control Pad).
COLCAM-HD
Color Video Camera
Includes 3m DV cable, power supply, C/CS mount.
504136
3 meter HDMI cable
504138
Hand held control pad
C-Mount Eyepiece Adapters
For 1/4-inch (4mm diagonal), 1/3-inch (6 mm diagonal) and 1/2-inch
(8 mm diagonal) video cameras and eyepiece camera conversion, this
lens and its accessories make it possible to connect a typical video or
C-mount camera to almost any microscope on the market. The lens fits
right into the ocular socket of standard 23.2 mm microscopes and the
30 mm adapter allows for use on the typical stereo zoom microscope.
If you already have a trinocular microscope you can add the included
C-Adapter to the top of an existing 1X C-mount (no lens) adapter. For a list
of 1X C-mounts for popular microscopes, visit WPI’s website and search for
“C-mounts”.
503097
503098 * Adapter, 0.45X for 1/3-in. and 1/2-in. video cameras, 30 mm Stereo adapter, 1X C-mount adapter
Adapter, 0.28X for 1/4-in. video cameras, 30 mm Stereo adapter, 1X C-mount adapter
* For adapter 503098 to mount properly, the 1/4-inch CCD camera
must have a minimum of 13 mm clearance, measured from the front
of the C-mount ring to the CCD surface.
SLR Digital Camera-to-Microscope
Eyepiece Adapter
This adapter connects T-mount SLR digital cameras to almost any
microscope on the market. The adapter is built to 23.2 mm ocular tubes
that are found on most high magnification (upright, inverted, standard)
microscopes. The 30 mm adapter allows mounting on most Stereo zoom
microscopes that use 30 mm oculars. If you already have a trincocular
microscope you can add this adapter to the top of an existing 1X C-mount
adapter.
The 2X magnification of this microscope adapter yields an approximate
65% field of view from the visual field as measured on a Canon 10D
Digital camera. (CCD Sensor size = 22.7 x 15.1 mm). 35 mm film
reference size is 24 x 36 mm.
Please contact your camera dealer for a suitable T-mount to bayonet
adapter for your camera.
503099
Adapter, 2.0X for SLR digital Cameras (includes 30 mm Stereo adapter, 1X C-mount adapter )
503097
t inc
o
ra n
e
Cam
d.
lude
503099
162
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
FluoroDish
™
Cover-glass bottom for
observing and growing cells
in imaging related research
l Optical quality glass bottom
for better imaging quality
(RI=1.525)
l Lowest access angle for
micropipette
l Low toxicity adhesive for
embryo research
WPI’s FluoroDish™ tissue culture dishes
are now available in a larger range of sizes
and coatings. These polycarbonate dishes
provide exceptional imaging quality for many
applications requiring the use of inverted
microscopes such as high resolution image
analysis, microinjection and electrophysical
recording of fluorescent-tagged cells. Taking
advantage of WPI’s extensive experience with
low toxicity adhesives, FluoroDish uses a
specially formulated adhesive that is optically
clear, durable and with extremely low toxicity.
Tests by an independent laboratory have shown
that the 96-hour surviving rate of embryos is
100% when kept in FluoroDish, substantially
better than some other brands. The bottom
glass has superior UV transmission (30%
transmission at 300 nm, compared to less
than 7% for the most popular German glass).
Stringent quality control ensures that glass
thickness stays within the 0.17 ±0.01 mm
range.
microscopy. Higher effective NA yields brighter
images for fluorescence and higher resolution
in Image Analysis. The glass bottom permits
the use of immersion objectives with medium
such as water, glycerin or oil for the highest
magnification possible. To optimize heatexchange, WPI’s glass-bottom dish is designed
to be flush (flat) with the microscope stage or
heating unit, therefore eliminating the air gap
that exists with modified plastic dishes in which
a glass cover slip has been inserted.
Three different sizes of FluoroDish are offered,
one type of 50 mm diameter dish and two
types of 35 mm diameter dishes. An inner well
is created within the dish by the glass bottom
and the tissue culture grade polystyrene which
forms the sides of the dish. All WPI dishes have
the advantages of low toxicity and good UV
transmission bottom glass. They are individually
packed and gamma sterilized.
Certain types of cell lines (e.g., PC3 and HEK)
adhere well to the uncoated glass bottom dish.
The poly-D-lysine coating has been reported to
improve the adhesion of neuron cells. The users
can also apply to the uncoated dish any special
coating that is best for their cell line.
The 10 mm glass window dish (FD3510) has
low sidewall for easy microelectrode access and
low solution volume. The low microelectrode
access angle is the lowest among all of 35
mm dishes on the market (very close that of
a 50 mm dish). The dish needs only 100 ~
200 mL to cover the bottom well, an important
feature when using expensive drugs and
chemicals.
The 50 mm dish (FD50) has a large growth area
(35 mm well diameter), a low access angle for
microelectrodes, and grips for easy handling.
The 35 mm dish has outside dimensions
similar to that of a Corning 35 mm dish
and has ø23.5 mm glass window (FD35)
or ø10 mm glass window (FD3510). Most
heaters and perfusion adapters designed
for the Corning 35 mm dish will also fit this
dish. The 23.5 mm glass window dish is
available uncoated or poly-D-lysine-coated.
OD
ID
Conventional plastic dishes and chambers
limit the utility of the inverted scope for many
Access
h
applications because the thick plastic bottom
Angle
H
requires a long working distance objective
available only in lower magnifications. Each WPI
dish has a flat (0.17 mm thick) optical quality
Glass Ø
glass bottom, allowing
the use of a much
Part Number
ID (mm
OD (mm)
Glass Ø (mm)
height (inside) Height (outside) Access Angle
shorter objective working FD35
33
35.5
23.5
7.8
9
29°
distance, larger numerical FD3510
10
35.5
10
1.5
4.65
17°
aperture (NA), and a
FD5040
47.5
49.82
35
7.25
7.4
17°
higher magnification (up
to 100x). The larger NA
and higher magnification
provide superior
FD35-100
FluoroDish Sterile Culture Dish, clear wall, 35 mm, 23 mm well, box of 100
quality imaging
FD35PDL-100 FluoroDish Sterile Culture Dish, Poly-D-Lysine Coated, clear wall, 35 mm, 23 mm well, box of 100
for both classical
FD3510-100 FluoroDish Sterile Culture Dish, clear wall, 35 mm, 10 mm well, low sidewall, box of 100
and fluorescence
FD5040-100 FluoroDish Sterile Culture Dish, clear wall, 50 mm, 35 mm well, box of 100
l Choose from poly-D-lysine or collagen coated, or uncoated
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
163
MICROSCOPES, CAMERAS
l Low sample volume for
expensive chemicals
MICROSCOPES, CAMERAS
Cover
Slips
Order Number
Diameter
Thickness
Quantity
502040
5 mm
#1.5 (0.16 - 0.19 mm)
100
502041
8 mm
#1.5 (0.16 - 0.19 mm)
100
503508
25 mm
#1.5 (0.16 - 0.19 mm)
100
Slides
These clean glass microscope slides are 25 x 75 mm,
1.0~1.2 mm thick with 90° grounded edges. and are available
plain, frosted, and red ended. The frosted end slides feature
503505
503505
503506
503507
164
These cover slips made of German glass
can be used for growing and culturing
cells that normally have poor adhesion to
plastic surfaces. They are small enough
to be placed in the micro plate or other
cell culture devices. The 5 mm size will fit
inside the 96-well culture plate and leave
enough room to pick it up from the bottom
of the well with forceps. The 8 mm size fits
inside the 24-well plates.
a fine 20 mm frosted area on both sides of one end for easy
marking. The red frosted slides feature a 20 mm colored end
useful for identifying hazardous materials.
503507
503506
Plain Glass Microscope Slides, Box of 144 Frosted Glass Microscope Slides, Box of 144
Red frosted Glass Microscope Slides, Box of 144
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
164
Fluid Range
Channels
Special features
Page
Peristaltic Pumps
Peripro-2HS
Peripro-4HS
Peripro-4LS
Peripro-8LS
MityFlex
MINISTAR
0.8 - 300 mL/min
0.8 - 300 mL/min
0.01-80 mL/min
0.01-80 mL/min
1.7-800 mL/min
0.006- 37 mL/min
2
4
4
8
1
1
Calibrated output, replaceable tubing cartridges
Calibrated output, replaceable tubing cartridges
Calibrated output, replaceable tubing cartridges
Calibrated output, replaceable tubing cartridges
High volume
Compact design, remote control
166
166
166
166
168
168
Single-Channel and Whole-Cell Solution Exchange
MPS2
Up to 250 µL/min
8
Programmable control; low dead volume
169
Infuse only
Infuse only
Infuse/Withdraw
Infuse/withdraw
Infuse/withdraw programmable
Push-pull
Push-pull programmable
Infuse only
Basic single channel
Micro dialysis application
RS232 TTL/Footswitch
RS232 Infuse Only
RS232 Infuse Only
RS232 Infuse/Withdraw
RS232 Infuse/Withdraw
Push pull, single cycle
RS232 push pull, single cycle
RS232 push pull, continuous
Push/pull
Push/pull (2 networked pumps)
170
171
171
172
172
172
172
173
174
176
176
174
176
175
176
176
175
175
177
177
Laboratory Syringe Pumps
SPLG100
SPLG101
SPLG110
SPLG210
SPLG212
SPLG270
SPLG272
SPLG200
SP100i
SP101i
SP200i
SP220i
SP250i
SP210iw
SP230iw
SP120p
SP260p
SP210c
AL-1000
AL-2000
1.26 pL/min to 88.32 mL/min
1.26 pL/min to 25.99 mL/min
1.26 pL/min to 88.28 mL/min
0.5 pL/min to 220.97 mL/min
0.5 pL/min to 220.97 mL/min
0.5 pL/min to 220.97 mL/min
0.5 pL/min to 220.97 mL/min
0.5 pL/min to 220.97 mL/min
0.0001-519 mL/hr
0.001 µL/hr – 35mL/min
0.001 µL/hr - 145 mL/min
0.001 µL/hr - 21 mL/min
0.001 µL/hr - 21 mL/min
0.001 µL/hr - 145 mL/min
0.001 µL/hr - 21 mL/min
0.1 µL/hr – 127 mL/hr
0.001 µL/hr - 86 mL/min
0.001 µL/hr - 86 mL/min
0.000073-1699 mL/hr
0.000073-1699 mL/hr
1
2
1
2
2
2
2
2
1
2
2
10
4
2
10
1+1
2+2
2+2
1
2
Micro Syringe Pump / Stereotaxic Injection
UMP3
MMP
DMP
0.03nL/min - 10 µL/sec
Manual 100 µL-1mL syringe
Manual 100 µL-1mL syringe
1
1
1
Ultra micro infuse/withdraw RS232
Manual
Digital readout micrometer
182
187
187
1
Oocyte injector, infuse only
186
Oocyte Injection
NANOLITER2010
Bolus, 2.3-69 mL/Injection
Single and Multi-channel Micro Pressure Injection
PV820
PV830
Bolus/Continuous Pressure,
Duration and Pipette dependent
Bolus/Continuous Pressure,
Duration and Pipette dependent
1
Injection pressure and holding pressure
184
1
Injection pressure and holding pressure and vacuum
184
1
Infuse only
191
Microfluidics
NEW
SF-MFP
0.000073-1699 mL/hr
Supplies & Tools
NEW
NEW
187
188
190
178
179
180
181
183
Pressure Manometers
NanoFil™ Specialty Microsyringe
NanoFil™ Application Kits
LM Series Pipetters — Standard and Autoclavable
ClickPet Repeating Pipetters
Regal Pipetters
Universal Pipette Tips
Syringes, Microvolume, for UltraMicroPump III
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
165
PUMPS, MICROINJECTION
NEW
NEW
NEW
NEW
NEW
NEW
NEW
NEW
Peri-Star Pro
™
High performance digital peristaltic pump at an
affordable price!
l Display either rotation speed
(RPM) or flow rate (mL/min)
PUMPS, MICROINJECTION
l Wide flow range: 0.01 280 mL/min
l Accuracy of flow rate: 0.5%
using self calibration function
l Accuracy of speed: 0.1 rpm
l Large backlit digital LCD
display
l Programmable for all tubing
sizes between 0.8 mm and
6.4 mm ID
l Easy and fast tubing
replacement using snap-on
cartridges
l Membrane keypad allows easy
programming while protecting
controls from fluid entry
l Actively driven rollers by
planetary gears for long
lasting tubing life
Peri-Star™ Pro peristaltic pumps provide
accurate and precise pumping with convenience
and versatility. Peri-Star Pro can be run in either
flow rate mode (mL/min) or rotation speed
mode (rpm). For good laboratory practice,
pumps must be calibrated after changing the
tubing and solution. Users can easily calibrate
Peri-Star Pro to deliver flow as accurate as
0.5% in a wide flow range from 0.01 mL/min
to 280 mL/min. Under rotation speed mode,
the digitally controlled stepping motor provides
accurate and reproducible operation with 0.1%
rpm both forward and in reverse.
Large backlit digital LCD display provides
readouts of rotation direction, flow rate or
rotation speed, tubing ID, drive status and
166
remote control mode simultaneously. Water
resistant membrane keypad allows easy
programming while protecting LCD display and
controls from fluid entry.
Built-in Human Machine Interface (HMI) with
screen instructions in plain English steps users
through initial setup, calibration and operating
procedures. The user-friendly interface reduces
the need to frequently check the printed manual
for instruction and reference.
Peri-Star Pro is available in two versions: a
4-roller version for high flow and an 8-roller
version for lower volumes which provides high
pressure with minimal pulsations.
A unique planetary gear design with eight
actively driven rollers (four rollers for higher
flow rate model), together with independent
tubing compression fine adjustment, greatly
increases flow accuracy and prolongs tubing
life. Snap-on cartridges allow tubing to be
changed quickly without cross contamination of
solutions.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Available in 2-, 4‑ and 8-channel versions
Pump head for
PERIPRO-4LS
(4 channels, 8 rollers)
Pump head for
PERIPRO-4HS
(4 channels, 4 rollers)
Pump head for
PERIPRO-8LS
(8 channels, 8 rollers)
Peri-Star PRO Specifications
Peri-Star Pro 2H / 4H
(High Rate)
Number of rollers
Number of channels
Rotor Speed Range
Fluid Flow range
Tubing range
SELF-CALIBRATION
Working Environment
Power
Dimensions
Shipping weight
Peri-Star Pro 4L / 8L
(Low Rate)
4
8
2–4
4–8
1–100 rpm
1–100 rpm
0.8–280 mL/min
#17 Tubing: 3.5–280 mL/min
0.01–80 mL/min
#14 Tubing: 0.2–18 mL/min
3.1–6.4 mm ID
0.5–2.4 mm ID
Yes
Yes
0–45°C, Humidity < 80%
0–45°C, Humidity < 80%
110 V or 220 V AC, 50 - 60 Hz
110 V or 220 V AC, 50 - 60 Hz
190 x 162 x 275 mm
190 x 162 x 275 mm
11 lb / 5 kg
11 lb / 5 kg
NEW PERI-STAR PRO PUMPS
PERIPRO-2HS Peri-Star™ Pro, 2-channel, High Rate, Large Tubing (110-220V)
PERIPRO-4HS Peri-Star™ Pro, 4-channel, High Rate, Large Tubing (110-220V)
PERIPRO-4LS Peri-Star™ Pro, 4-channel, Low Rate, Small Tubing (110-220V)
PERIPRO-8LS Peri-Star™ Pro, 8-channel, Low Rate, Small Tubing (110-220V)
OPTIONAL ACCESSORIES
503049Replacement Tubing Cartridge, Large
503050Replacement Tubing Cartridge, Small
503022
Replacement Silicone Tubing, 1m, 1.6 mm I.D., #14, with stops
503023
Replacement Silicone Tubing, 1m, 6.4 mm I.D., #17
503120
TTL Control Module
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
PUMPS, MICROINJECTION
Pump head for
PERIPRO-2HS
(2 channels, 4 rollers)
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
167
MityFlex
Peristaltic Pump
The MityFlex is an economical, variable speed, peristaltic pump that can
handle a wide variety of viscosities from air to gases to heavy slurries,
with consistent displacement.
This pump can be used for fluid transfer or for metering applications. By
varying the tubing a wide range of flow rates can be achieved. This is a
great value for medium to high flow applications.
MITYFLEX Specifications
NUMBER OF Rollers2
Flow rate
1.7 to 800 mL/min. Variable speed-reversible
RPM range8-228
Flow rates
Tubing I.D.
Flow Rate
1
/16 in.
1
/8 in.
3
/16 in.
1
/4 in.
1.7-48 mL/min*#14
7-190 mL/min
#16
16-456 mL/min
#25
28-800 mL/min
#17
POWER
115 VAC / 60 Hz or 220 VAC / 50 Hz
Dimensions
22.6 x 14.2 x 19 cm (8.9 x 5.6 x 7.5 in.)
Shipping Weight
3.6 kg (8 lb)
PUMPS, MICROINJECTION
* To use
Included with the MityFlex are four short lengths of Norprene™ tubing to fit into the
roller mechanism — 1/16", 1/8", 3/16", 1/4" — and black rollers for use with 3/16" to 1/4"
tubing. The optional MITY-KIT includes replacement tubing and a set of red rollers for
use with 1/16" and 1/8" tubing. Norprene is a trademark of Saint-Gobain Performance Plastics Corp.
Masterflex Equiv.
1
/8 -in. or 1/16 -in. tubing, MITY-KIT is required.
500282
MityFlex Peristaltic Pump (110 V) 500283
MityFlex Peristaltic Pump (220 V)
MITY-KIT Replacement Parts Kit with Rollers
MITY-KIT includes 4 short lengths of Norprene™ tubing to fit into the roller
mechanism — 1/16", 1/8", 3/16", 1/4" — and red rollers for use with 1/16" tubing.
Mini★Star™
Miniature DC Peristaltic Pump
This compact and lightweight peristaltic pump fits just about
anywhere. It can be mounted directly on the bench, in a regular
rack or to a post. The MiniStar’s speed can be adjusted from 1rpm
to 50 rpm. With recommended silicone tubing, the volume can be
set from 0.06 mL/min to 14.0 mL/min. The MiniStar also features
a hand held remote control that allows users to start and stop the
pump, purge or adjust its speed and direction.
MINISTAR SPECIFICATIONS
Channel1
Speed
1-50.0 rpm, forward/reverse
Flow range
0.06~14.0 mL/min
Resolution
1 rpm (0.1 rpm computer control)
Speed control
Remote control
Display
Indicators for status and speed
Power
12 V DC (110/220 VAC adapter incl.)
Working condition
Temperature 0-40°C, humidity < 80%
Tubing
Wall Thickness
Outer Diameter
Two-stop Silicone
0.8~1.0 mm
≤ 4.8mm
Dimension of driver 135×72×72 mm (L×W×H)
Dimension of remote control 105×50×16 mm (L×W×H)
Weight of driver
MINISTAR
503120
503121
503122
168
0.5 Kg
Miniature Peristaltic Pump, 1-channel
TTL Control Module
Silicone Tubing w stops, 2.4mm ID x 0.8mm wall x 1 m (5-pk)
Silicone Tubing w stops, 1mm ID x 1mm wall x 1 m (5-pk)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
MPS-2
Multichannel
Perfusion
System
● Manual or Programmable PC
control with user-friendly GUI
interface
● Fast LAFF solenoid valve
● Color-coded polyurethane tubing
for easy identification
for single ion channel and
whole-cell solution exchange
● Super low dead volume
(<100 nL) micromanifold
● Economically priced
inputs from 1/16” ID tubing
output: 100
or 250 µm ID
quartz/polymide
tubing
mounting rod
connector
14 mm OD
internal junction
OD: 600 µm
Micromanifold closeup: Fluid-filled passages are shown in magenta.
MPS-2 is a programmable 8-channel perfusion system designed for single
channel and whole-cell patch preparations. Offering the best combination
of performance and value, the MPS-2 incorporates the same high quality
solenoid valves found on similar but much more expensive systems.
Unlike other perfusion systems on the market, which often compromise
performance to fit every possible application, the MPS-2 is the only perfusion
system designed and optimized specifically for single-channel and whole-cell
patch perfusion applications.
The system can be controlled manually via membrane switches on the front
panel or through a PC. Two different manual control modes are offered. One
controls each channel independently and the other mode allows the user
to assign a master channel that will keep the system flow when all other
channels are switched off. User-friendly graphic timing software is included,
and the programmed perfusion sequence can be started by computer, a
patch clamp amplifier or other external trigger, or manually by the user
The perfusion fluid flows through specially designed color-coded
polyurethane ribbon style tubing. The color-coding allows the user to easily
trace each channel for diagnostic checks or set up and
MPS-2
Multichannel Perfusion System & Control Software
the ribbon style of tubing keeps the system very neat and
Replacement Parts
organized. Unlike PVC based tubing, polyurethane tubing
502109-15 Color-coded Polyurethane Tubing, 1/16” ID x 8 Channels, 15 ft
contains no plasticizer, which can cause contamination.
502110
Micromanifold, 100 µm ID tip, 2 pcs/pk
The tubing ribbon is designed as an economical
502125
Micromanifold, 200 µm ID tip, 2 pcs/pk
disposable item, which is often critical when cleanness is
needed.
Specify line voltage and Micromanifold tip OD when ordering.
MPS-2 SPECIFICATIONS
Channels8
Valve Response Time
2 ms
Valve Control
USB, TTL, external start via software
Syringe Reservoir Volume
10 mL
Manifold
8 to 1
Tip ID
250 micron and 100 micron.
Maximum Flow Rates (gravity fed)
100 µm ID tip, 8 µL/min. at 50 cm
250 µm ID tip, 250-500 µL/min. at 50 cm
Dead Volume
< 100 nL excluding the single outlet tubing
The most unique feature of the MPS-2 is its perfusion
micromanifold. Using the latest microfluidic techniques,
the injection molded micromanifold provides the least
flow resistance and dead volume of any product on the
market. The flow channel inner diameter is approximately
1.0 mm, except for the last 5 mm before the junction
point. This design allows a fast flow rate without using a
pressured system. The maximum flow rates are 1 and 16
microliter per second for the 15 mm long 100 µm and
250 µm ID tips, respectively. Small channels and a unique
design at the merging point further reduce the chance of
cross contamination. Dead volume is less than 100 nL.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
169
PUMPS, MICROINJECTION
internal
passage ID:
250 µm
Legato Syringe Pumps
The large touch screen color display lets you see all of the
pump’s operating parameters to ensure proper operation
during the experiments. Syringe size and flow rate are
easily displayed as well as the volume delivered and
elapsed time. Set up is easy using the icon-driven software.
An LED on the front panel makes it easy to see if the
pump is running. Advanced micro stepping techniques are
employed to further reduce the step angle to eliminate
flow pulsation. Accuracy is ±0.5%. A wide dynamic flow
range from picoliters per minute to millimeters per minute
can be programmed into the pump. These versatile pumps
can be connected through an RS485 interface. Add the
new Adagio software to maximize the use of the pump’s
functions and features. Adagio allows you to configure
the pump through the software as well as operate one
or multiple pumps. LabVIEW drivers are available on the
National Instruments website.
PUMPS, MICROINJECTION
Benefits
• Automatic dispensing of small volumes
• Constant delivery of fluids
• Hands free operation
• Better flow performance
Features
• Accuracy ±0.35%
• Holds one or two syringes from
0.5 μL to 140 mL
• High resolution color touch screen
• Real time clock
• Unparalleled ease of use
• Touch pad "lock" feature
• LED light on front panel
• Full metal chassis
• Built in syringe table
• Up to 75 lb linear force
SPLG100
Infuse-Only Syringe Pump
The SPLG100 is the world’s first single-syringe infusion-only pump
with a touchscreen interface. The SPLG100 has a wide flow rate range
from 1.26 pL/min to 88.32 mL/min, depending on syringe size. It
accommodates a single syringe from 0.5 µL to 60 mL. Any type of syringe,
including glass, plastic or stainless steel, are held securely in place.
SPLG100
504578
Legato 100 Syringe Pump, Infuse-Only
Software Adagio/Usb Key
SPLG100 SPECIFICATIONS
• Advanced microstepping
techniques
• Built in RS-485 interface to link
multiple pumps
• USB port & RS232 Interface
• I/O & TTL interface
• Continuous mode of operation
• Protection with a spill dam
• Analog control option
• CE, UL, CSA, CB Scheme, EU RoHS
FLOW RATES
Syringe
Diameter
Minimum
Maximum
0.5 μL
0.103 mm
1.260 pL/min
1.325 μL/min
1 μL
0.146 mm
2.520 pL/min
2.651 μL/min
2 μL
0.206 mm
5.100 pL/min
5.299 μL/min
5 μL
0.343 mm
14.100 pL/min
14.690 μL/min
10 μL
0.485 mm
28.260 pL/min
29.380 μL/min
25 μL
0.729 mm
63.900 pL/min
66.370 μL/min
Syringe Size
0.5 μL to 60 mL
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
Motor Drive Control
Microprocessor with 1/16 microstepping
50 μL
1.03 mm
127.600 pL/min
132.500 μL/min
Linear Force (Maximum)
13.6 kg (30 lb) @ 100% Force Selection
100 μL
1.457 mm
255.20 pL/min
265.100 μL/min
Number of Microsteps per One
Revolution of Lead Screw
15,360
250 μL
2.304 mm
638.300 nL/ min
662.900 μL/min
Step rate (Minimum)
27.5 sec/μstep
500 μL
3.256 mm
1.275 nL/min
1.324 mL/min
Step rate (Maximum)
26 μsec/μstep
1000 μL
4.608 mm
2.553 nL/min
2.652 mL/min
Drive Motor
0.9 degree Stepper Motor
1 mL
4.699 mm
2.655 nL/min
2.757 mL/min
Pusher Travel Rate (Minimum)
0.15 μm/min
Pusher Travel Rate (Maximum)
159 mm/min
3 mL
8.585 mm
8.863 nL/min
9.204 mL/min
Flow Rate (Minimum)
1.26 pL/ min (0.5 μL syringe)
5 mL
11.989 mm
17.290 nL/min
17.950 mL/min
Flow Rate (Maximum)
88.32 mL/min (60 mL syringe)
10 mL
14.427 mm
25.030 nL/min
25.990 mL/min
Dimensions
22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in)
20 mL
19.05 mm
43.640 nL/min
45.320 mL/min
Weight
2.66 kg (5.9 lb)
RS485 - IEEE-1394 6 pos,
USB Type B
30 mL
21.59 mm
56.050 nL/min
58.210 mL/min
Connectors
60 mL
26.594 mm
85.050 nL/ min
88.320 mL/min
170
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Superior reliability and flow performance
SPLG110
Infuse/Withdraw Syringe Pump
The SPLG101 is ideal for applications where dual syringes are required
with small volumes under 10mL. It accommodates two syringes from
0.5 µL to 10 mL. The SPLG101 has a wide flow rate range from 1.26 pL/
min to 25.99 mL/min, depending on syringe size.
The SPLG110 offers infuse/withdraw flow control and programmability
for up to two multi-step programs of 50 steps each. The SPLG110 has a
wide flow rate range from 1.26 pL/min to 88.28 mL/min, depending on
syringe size. The SPLG110 accommodates a single syringe from 0.5 µL to
60 mL. Any type of syringe can be used in the unit including glass, plastic
or stainless steel. The pump is ideal for more complex multi-step dosing
and has all multi-mode operation including infusion only, withdrawal only,
infusion and withdrawal and withdrawal/infusion modes.
SPLG101
504578
Legato 101 Syringe Pump, Dual Infuse-Only
Software Adagio/Usb Key
SPLG110
504578
Legato 110 Syringe Pump, Infuse/Withdraw
Software Adagio/Usb Key
SPLG101 SPECIFICATIONS
SPLG110 SPECIFICATIONS
Syringe Size
0.5 μL to 10 mL
Syringe Size
0.5 μL to 60 mL
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
Motor Drive Control
Microprocessor with 1/16 microstepping
Motor Drive Control
Microprocessor with 1/16 microstepping
Linear Force (Maximum)
13.6 kg (30 lbs) @ 100% Force Selection
Linear Force (Maximum)
13.6 kg (30 lbs) @ 100% Force Selection
Number of Microsteps per One
Revolution of Lead Screw
15,360
Number of Microsteps per One
Revolution of Lead Screw
15,360
Step rate (Minimum)
27.5 sec/μstep
Step rate (Minimum)
27.5 sec/μstep
Step rate (Maximum)
26 μsec/μstep
Step rate (Maximum)
26 μsec/μstep
Drive Motor
0.9 degree Stepper Motor
Drive Motor
0.9 degree Stepper Motor
Pusher Travel Rate (Minimum)
0.15 μm/min
Pusher Travel Rate (Minimum)
0.15 μm/min
Pusher Travel Rate (Maximum)
159 mm/min
Pusher Travel Rate (Maximum)
159 mm/min
Flow Rate (Minimum)
1.26 pL/ min (0.5 μL syringe)
Flow Rate (Minimum)
1.26 pL/ min (0.5 μl syringe)
Flow Rate (Maximum
25.99 mL/min (10 mL syringe)
Flow Rate (Maximum)
88.28 mL/min (60 ml syringe)
Dimensions
22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in)
Dimensions
22.6 x 19.05 x 15 cm (9 x 7.5 x 5 in)
Weight
2.66 kg (5.9 lbs)
Weight
2.66 kg (5.9 lbs)
Connectors
RS-232 - 9 Pin D-Sub Connector,
RS485 - IEEE-1394 6 pos,
USB - Type B
Connectors
RS-232 - 9 Pin D-Sub Connector,
RS485 - IEEE-1394 6 pos,
USB - Type B
Adagio Software
l Low Cost Simple Installation l Flow Evolution Graph l Import & Export Programs l Quick & Easy
Manual Pump Control l Monitor One or More Pumps l Program Data Logging
The manual pump control tool allows easy direct control of the pump. Pump commands can be
entered directly into the log. Multiple programs can be opened at the same time. The program’s
progression is tracked, and can be stored in a file for later access.
System Requirements:
Intuitive Run Screen — Combining multiple parameters
simultaneously with internationally recognizable icons
allow the Legato™ Series to provide a new level of
intuitive syringe pump operation.
l 1 GHz Pentium processor or higher l 512 MB of RAM (1 GB recommended) l Windows XP SP3 or Vista
(XP recommended) l Free RS232 or USB 2.0 ports (depending on the quantity, model and connectivity of the
controlled pumps; daisy chained pumps require a single port; direct PC-to-Pump connections require one free port
per pump) l Microsoft Excel 97 or higher
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
171
PUMPS, MICROINJECTION
SPLG101
Dual Infuse-Only Syringe Pump
PUMPS, MICROINJECTION
Legato Syringe Pumps
SPLG210
Infuse/Withdraw Syringe Pump
SPLG270
Infuse/Withdraw Continuous
The SPLG270 is a Push-Pull syringe pump. It accommodates two syringes
from 0.5 μL to 140 mL for infusion and two syringes for withdrawal. This
model supports infusion and withdrawal simultaneously at user-defined flow
rates and with selectable target volumes to control the total volume pumped.
It also supports infuse only, withdraw only, infuse/withdraw, withdraw/infuse
and continuous mode. The touch screen interface lets you quickly create
configurations and recall them for easy use. The 4.3-inch TFT color display
with touch pad interface presents all the pump operating parameters on one
easy-to-view run screen. Protective cover over the display prevents leakage into
the display.
SPLG270 SPLG272
504576
504577
504578
SPL Syringe Pump, Push-Pull SPL Syringe Pump, Push-Pull Programmable
Small Syringe Multi Rack (for six 30-60mL syringes or ten 0.5µL-20mL syringes)
Large Syringe Multi Rack (for up to four 60-140 mL plastic syringes)
Software Adagio/Usb Key
The SPLG210 Infuse/Withdraw syringe pump offers unparalleled ease of use
through the high resolution touch screen. The basic model works with one
syringe or two (from 0.5µL to 140mL) and can be reconfigured in the field to be
used with multiple syringes. rotective cover over the display prevents leakage
into the display. To optimize your bench space the SPLG210 can be placed on
its side to reduce the footprint to only 3.5 in. x 9.75 in. The display also tilts
with the change to allow the user to operate the pump vertically.
The programmable model offers maximum flexibility for configuring and
running different programs. Up to 40 programs of 20 steps each can be
configured and stored in the unit and recalled quickly with the touch of a
button.
SPLG210 SPLG212 504576
504577
504578
SPLG270 SPECIFICATIONS
SPL Syringe Pump, Infuse/Withdraw
SPL Syringe Pump, Infuse/Withdraw Programmable
Small Syringe Multi Rack (for six 30-60mL syringes or ten 0.5µL-20mL syringes)
Large Syringe Multi Rack (for up to four 60-140 mL plastic syringes)
Software Adagio/Usb Key
SPLG210 Specifications
Syringe Size
0.5 μL to 140 mL
Syringe Size
0.5 μL to 140 mL
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
Motor Drive Control
Microprocessor with 1/16 microstepping
Motor Drive Control
Microprocessor with 1/16 microstepping
Linear Force (Maximum)
34 kg (75 lbs) @ 100% force selection
Linear Force (Maximum)
34 kg (75 lbs) @ 100% force selection
Number of Microsteps per One
Revolution of Lead Screw
6400
Number of Microsteps per One
Revolution of Lead Screw
6400
Step rate (Minimum)
27.5 sec/μstep
Step rate (Minimum
27.5 sec/μstep
Step rate (Maximum)
26 μsec/μstep
Step rate (Maximum)
26 μsec/μstep
Drive Motor
1.8 degree Stepper Motor
Drive Motor
1.8 degree Stepper Motor
Pusher Travel Rate (Minimum)
0.36 μm/min
Pusher Travel Rate (Minimum)
0.36 μm/min
Pusher Travel Rate (Maximum)
190.80 mm/min
Pusher Travel Rate (Maximum
190.80 mm/min
Flow Rate (Minimum)
5 pL/min (0.5 μL syringe)
Flow Rate (Minimum)
5 pL/min (0.5 μL syringe)
Flow Rate (Maximum)
215.803 mL/min (140 mL syringe)
Flow Rate (Maximum)
215.803 mL/min (140 mL syringe)
Dimensions
8.89 x 25.4 x 27.94 cm (3.5 x 10 x 11 in)
Dimensions
8.89 x 25.4 x 27.94 cm (3.5 x 10 x 11 in))
Weight
4.9 kg (10.75 lb)
Weight
4.9 kg (10.75 lb)
Connectors
RS-232 - 9 Pin D-Sub Connector,
RS-485 - IEEE-1394 6 pos,
USB - Type B,
I/O & TTL - 15 Pin D-Sub Connector
Connectors
RS-232 - 9 Pin D-Sub Connector,
RS-485 - IEEE-1394 6 pos,
USB - Type B,
I/O & TTL - 15 Pin D-Sub Connector
172
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Superior reliability and flow performance
When mounted vertically,
the display screen of
the SPLG series pumps
automatically reorients for
ease of use.
PUMPS, MICROINJECTION
SPLG200
Infuse-Only Syringe Pump
The SPLG200 Infuse only syringe pump offers unparalleled
ease of use through the high resolution touch screen. The basic
model works with one syringe or two (from 0.5 μL to 140 mL ) and can be
reconfigured in the field to be used with multiple syringes. To optimize your
bench space the SPLG200 can be placed on its side to reduce the footprint to
only 3.5 x 9.75 inches. the display also tilts with the change to allow the user
to operate the pump vertically. User definable flow rates with selectable target
volumes or time values to control the total infusion volume. Up to 40 programs
of 20 steps each can be configured and stored in the unit and recalled quickly
with the touch of a button.
SPLG200
504576
504577
504578
SPL Syringe Pump, Infuse Only
Small Syringe Multi Rack (for six 30-60mL syringes or ten 0.5µL-20mL syringes)
Large Syringe Multi Rack (for up to four 60-140 mL plastic syringes)
Software Adagio/Usb Key
SPLG200 Specifictions
SPLG200 FLOW RATES
Syringe
Diameter
Minimum
Maximum
0.5 μL
0.103 mm
3.12 pL/min
1.589 μL/min
1 μL
0.146 mm
6.18 pL/min
3.180 μL/min
2 μL
0.206 mm
12.301 pL/min
6.358 μL/min
5 μL
0.343 mm
33.96 pL/min
17.630 μL/min
10 μL
0.485 mm
67.72 pL/min
35.249 μL/min
Syringe Size
0.5 μL to 140 mL
25 μL
0.729 mm
153.42 pL/min
79.640 μL/min
Power
100-240 VAC: 50/60 Hz, 50W. 0.5 A fuse
50 μL
1.03 mm
306.24 pL/min
158.984 μL/min
Motor Drive Control
Microprocessor with 1/16 microstepping
Linear Force (Maximum)
34 kg (75 lbs) @ 100% force selection
100 μL
1.457 mm
612.72 pL/min
318.126 μL/min
Number of Microsteps per One
Revolution of Lead Screw
250 μL
2.304 mm
1.533 nL/ min
795.51 μL/min
6400
500 μL
3.256 mm
3.06 nL/min
1.588 mL/min
Step rate (Minimum)
27.5 sec/μstep
1000 μL
4.608 mm
6.129 nL/min
3.181 mL/min
Step rate (Maximum)
26 μsec/μstep
Drive Motor
1.8 degree Stepper Motor
1 mL
4.699 mm
6.373 nL/min
3.308 mL/min
Pusher Travel Rate (Minimum)
0.36 μm/min
3 mL
8.585 mm
21.272 nL/min
11.044 mL/min
Pusher Travel Rate (Maximum)
190.80 mm/min
5 mL
11.989 mm
41.485 nL/min
21.539 mL/min
Flow Rate (Minimum)
5 pL/min (0.5 μL syringe)
10 mL
14.427 mm
60.073 nL/min
31.19 mL/min
Flow Rate (Maximum)
215.803 mL/min (140 mL syringe)
Dimensions
8.89 x 25.4 x 27.94 cm (3.5 x 10 x 11 in.)
20 mL
19.05 mm
104.74 nL/min
54.383 mL/min
Weight
4.9 kg (10.75 lb)
30 mL
21.59 mm
134.533 nL/min
69.852 mL/min
Connectors
RS-232 - 9 Pin D-Sub Connector,
S485 - IEEE-1394 6 pos,
USB - Type B,
I/O & TTL - 15 Pin D-Sub Connector
50 mL
26.594 mm
204.122 nL/ min
105.985 mL/min
100 mL
35.7 mm
367.839 nL/min
190.992 mL/min
140 mL
38.4 mm
415.623 nL/min
215.803 mL/min
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
173
SP Series Syringe Pumps
Microprocessor-controlled syringe pumps for applications requiring high me­ter­ing
precision at low, pulse-free rates
Sturdy and reliable, ex­treme­ly sim­ple to set up and use — and sur­pris­ing­ly af­ford­able. Liquid crys­tal
displays (LCDs) prompt you through set­up:
1. Select syringe from table stored in the pump’s memory and dis­played on the LCD.
2. Enter the volume to be dispensed.
SP100i SPECIFICATIONS
3. Enter the flow rate, press “start.”
PUMPS, MICROINJECTION
It’s fast and simple. Your settings are permanently stored in memory — there’s no need to re-enter
them each day. SP pumps feature preset rate and volume control. Just set the volume you want
dispensed. Volume is tracked continuously on the LCD display. Then, when the preset volume has
been dispensed, the pump shuts off automatically. The easy-to-read digital dis­play provides real-time
read­ings using both parameters and val­ues for clearer, mistake-free readings. The SP200 Series
pumps offer TTL and RS-232C interfaces and automatic shutoff under stall conditions.
Single-Syringe Infusion
Pump
SP100i
ModeInfusion
Syringe Size
10 µL to 60 mL (one)
Maximum Flow Rate
519 mL/hr
(60 mL Syringe)
Minimum Flow Rate0.1µL/hr
(10 µL Syringe)
Linear Force
20 Ib (9 kg)
Advance Per Microstep
0.529 micron (1/2-step)
Maximum Step Rate
400 steps/sec
(1/2-step)
This inexpensive single-syringe infusion pump
combines precision and simplicity with
outstanding ease of use and durability.
● Holds any size syringe, 10 µL to 50 mL
● Automatic volume control and shutoff
● Simple menu-driven setup: dispense volume,
dispense flow rate, syringe diameter (selected from
displayed table)
Minimum Step Rate
1 step/30 sec
Accuracy
< 1% error
Reproducibility
± 0.1%
Dimensions
9 x 6 x 5.5 in.
23 x 15 x 14 cm
Shipping Weight
7.5 Ib (3.4 kg)
Available 95-135 V or 220-240 V, 50/60 Hz
● Last settings stored in permanent memory
● Continuous dispense volume display
SP220i SPECIFICATIONS
SP220i
ModeInfusion
Syringe Size
10 µL to 10 mL (ten)
10 µL to 60 mL (six)
10 µL to 140 mL (four)*
Maximum Flow Rate
21 mL/min (10 mL syringe)
Minimum Flow Rate
0.001µL/hr (10 µL Syringe )
Linear Force
40 lb (18 kg)
Advance Per Microstep
0.165 micron
Maximum Step Rate
1600 steps/sec (1/2-step)
Minimum Step Rate
1 step/100 sec
Accuracy
< 1% error
Reproducibility
± 0.1%
Dimensions
11 x 12 x 5.5 in.
28 x 30 x 14 cm
Shipping Weight
12.5 Ib (5.7 kg)
Multi-Syringe Infusion Pump
SP220i
Ideal for applications requiring multiple
syringes, the SP220i is an adaptation of
the SP200i and has been modified
to hold up to 10 syringes.
● SP220i has all features of
SP200i
● The multiple syringe holder
accommodates 10 syringes
up to 10 mL, or six syringes
up to 50 mL, or four
syringes up to 140 mL.
Available 95-135 V or 220-240 V, 50/60 Hz
SP100i
SP100iZ
SP220i
SP220iZ
####-A
####-P
174
Syringe Pump, Infusion (single), 95-135 V
Syringe Pump, Infusion (single), 220-240 V
Syringe Pump, Infusion (multiple), 95-135 V
Syringe Pump, Infusion (multiple), 220-240 V
All 240-volt pumps are CE-approved.
Audible Alarm (add “A” to pump part number when ordering)
Programmable Ramp Option (SP200 Series) OPTIONAL CABLES
15623
Serial cable, SP Pump-to-IBM 9-pin “D” connector
15624
Serial cable, SP Pump-to-Macintosh connector
15676
Serial cable, SP Pump-to-IBM 25-pin “D” connector
13685
SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft
13962
Footswitch for SP200 Series Pumps
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Two-Syringe Infusion/Withdrawal Pump
SP210iw
The SP210iw offers you more advanced features
than any other infusion/withdrawal pump in its
price range—including five operating modes
plus independent rate and volume settings
for both infusion and withdrawal.
SP210iw SPECIFICATIONS
ModeInfusion/Withdrawal
Syringe Size
10 µL to 140 mL (two)*
Maximum Flow Rate
145 mL/min (140 mL syringe)
Minimum Flow Rate0.001µL/hr
● All features of SP200i
● Independent rate and volume settings
for infusion and withdrawal
● Multiple mode selection: infusion,
withdrawal, infusion then
withdrawal, withdrawal then
infusion, or continuous cycle.
Linear Force
40 Ib (18 kg)
Advance Per Microstep
0.165 micron
Maximum Step Rate
1600 steps/sec
Minimum Step Rate
1 step/100 sec
Accuracy
< 1% error
Reproducibility
± 0.1%
Dimensions
11 x 9 x 5.5 in.
28 x 23 x 14 cm
Shipping Weight
12 Ib (5.4 kg)
Available 95-135 V or 220-240 V, 50/60 Hz
Four-Syringe Push-Pull Pump
SP260p single cycle
PUMPS, MICROINJECTION
The SP260p can hold up to four syringes. As two syringes are infusing, two other syringes
are withdrawing at the same rate. The SP260p is used for single-cycle applications only.
●Knob locks/unlocks drive block for ef­fort­less, drag-free ad­just­ment
●Simple menu-driven setup: Syringe di­am­eter (se­lect­ed from dis­played table), Dis­pense
vol­ume, Dis­pense flow rate
● Continuous dispense volume display
● Preset volume control and automatic shutoff
● Settings can be reviewed or changed dur­ing oper­a­tion
● Optical encoder stall detection
● Choice of unit selection
● Last settings stored in permanent mem­o­ry
● Built-in RS-232C interface for com­put­er link­ing or “dai­sy chain­ing” up to 100 pumps.
● TTL interface for foot switch, timer, re­lay con­trol; out­puts for run in­di­ca­tor, valve control
Continuous Cycle Syringe Pump
SP210c
The SP210c can hold up to four syringes and can
cycle continuously back and forth in a push-pull action.
SP260p & SP210c
SPECIFICATIONS
Mode
SP260pPush-Pull (single cycle)
SP210cInfusion/Withdrawal (continuous)
Syringe Size
SP260p
SP210c
10 µL-60 mL (four)
10 µL-60 mL (four)
Maximum Flow Rate
SP260p
SP210c
86 mL/min (60 mL syringe)
86 mL/min (60 mL syringe)
Minimum Flow Rate0.001µL/hr
Linear Force
40 Ib (18 kg)
Advance Per Microstep
0.165 µm
Maximum Step Rate
1600 steps/sec
Minimum Step Rate
1 step/100 sec
Accuracy
< 1% error
Reproducibility
± 0.1%
Dimensions
11 x 9 x 5.5 in.
28 x 23 x 14 cm
Shipping Weight
12 Ib (5.4 kg)
Available 95-135 V or 220-240 V, 50/60 Hz
As two syringes are infusing, two other syringes
are withdrawing at the same rate. At the end of
the set volume the direction is automatically
reversed and the next cycle begins. With the use
of 2-way valves, the pump can empty and refill
syringes for a continuous dispense.
●Holds four syringes, 10 mL to 60 mL
each. With larger syringes the full
volume may not be usable. [With a
60 mL syringe, 40 mL is usable; with a
30 mL syringe, the full volume is usable.]
SP210iw
SP210iwZ
SP260p
SP260pZ
SP210c
SP210cZ
Syringe Pump, Infusion & Withdrawal (double), 95-135 V
Syringe Pump, Infusion & Withdrawal (double), 220-240 V
Syringe Pump, Infusion-Withdrawal (double) Single Cycle Action, 95-135 V
Syringe Pump, Infusion-Withdrawal (double) Single Cycle Action, 220-240 V
Syringe Pump, Infusion & Withdrawl (Continuous Action), 95-135 V
Syringe Pump, Infusion & Withdrawl (Continuous Action), 220-240 V
All 240-volt pumps are CE-approved.
####-A
Audible Alarm (add “A” to pump part number when ordering)
####-P
Programmable Ramp Option (SP200 Series) OPTIONAL CABLES
15623
Serial cable, SP Pump-to-IBM 9-pin “D” connector
15624
Serial cable, SP Pump-to-Macintosh connector
15676
Serial cable, SP Pump-to-IBM 25-pin “D” connector
13685
SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft
13962
Footswitch for SP200 Series Pumps
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
175
Two-Syringe Microdialysis Pump
SP101i
Developed especially for use in microdialysis
experiments, these pumps produce very fine
syringe movement. The modified SP100i
gearing features a sixfold gear reduction
compared to standard models, allowing
pumping at much smaller flow rates. (See
sensors such as ISO-NO and Micro-C for
detection of dialysates.) The SP101i is a
dual barrel infusion only pump.
● Holds two syringes, 10 µL to 1­0 mL
PUMPS, MICROINJECTION
Two-Syringe Push-Pull Pump
SP120p
A second syringe mount
has been added to the basic
SP100i, with both syringes
activated by a single pusher
block for simultaneous
infusion and withdrawal. This
pump has all the features of
the SP100i, plus:
● Holds two syringes, from
10 µL to 10 mL.
Two-Syringe
Infusion Pump
SP200i
This feature-laden two-syringe infusion pump combines a broad speed range
and holds a wide range of syringe sizes to meet the requirements of virtually any
laboratory application.
● Holds two syringes, 10 to 140 mL
● Backlit LCD display
● Knob locks/unlocks drive block for effortless, drag-free adjustment
● Simple menu-driven setup: Syringe diameter (selected from displayed table),
Dispense volume, Dispense flow rate
● Continuous dispense volume display
● Preset volume control and automatic shutoff
● Settings can be reviewed or changed during operation
● Optical encoder stall detection
● Choice of unit selection
● Last settings stored in permanent memory
● Built-in RS-232C interface for computer linking or “daisy chaining” up to 100
pumps.
Four-Syringe Nanoliter Infusion Pump
SP250i
● TTL interface for foot switch, timer, relay control; outputs for run indicator, valve
control.
Each syringe can be sized differently
and is clamped independently
● Holds four syringes, up to 10 mL
each.
Multi-Syringe Infusion/Withdrawal
Pump
● Separate clamping accommodates
different sizes.
● Syringes may be positioned
independently for sequential
dispensing by the pusher block.
SP101i
Syringe Pump, Microdialysis (double, slow speed), 95-135 V
SP101iZ
Syringe Pump, Microdialysis (double, slow speed), 220-240 V
SP120pSyringe Pump, Infusion-Withdrawal (double), 95-135 V
SP120pZSyringe Pump, Infusion-Withdrawal (double), 220-240 V
SP200i
Syringe Pump, Infusion (double), 95-135 V
SP200iZ
Syringe Pump, Infusion (double), 220-240 V
SP230iw Syringe Pump, Infusion & Withdrawal (multiple), 95-135 V
SP230iwZ Syringe Pump, Infusion & Withdrawal (multiple), 220-240 V
SP250iSyringe Pump, Infusion (multiple, mixed volumes), 95-135 V
SP250iZSyringe Pump, Infusion (multiple, mixed volumes), 220-240 V
All 240-volt pumps are CE-approved.
####-A
Audible Alarm (add “A” to pump part number when ordering)
####-P
Programmable Ramp Option (SP200 Series) OPTIONAL CABLES
15623
Serial cable, SP Pump-to-IBM 9-pin “D” connector
15624
Serial cable, SP Pump-to-Macintosh connector
15676
Serial cable, SP Pump-to-IBM 25-pin “D” connector
13685
SP Pump-to-Pump “Daisy-Chain” linking cable, 7 ft
13962
Footswitch for SP200 Series Pumps
176
SP230iw
Ideal for applications requiring multiple syringes, the SP230iw is an adaptationas of
the SP210iw, and has been modified to hold up to 10 syringes.
● SP230iw has all features of SP210iw
● The multiple syringe holder accommodates 10 syringes up to 10 mL, or six
syringes up to 50 mL, or four syringes up to 140 mL.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Aladdin
Programmable Syringe Pump
● Versatile
● More Features
● Dual Pumping Action
ALADDIN SPECIFICATIONS
AL-1000
AL-1000HP
SYRINGE SIZES
1-60 mL, or 0.5-5 µL micro syringes Up to 60 mL
NUMBER OF SYRINGES
1
MOTOR TYPE
Step Motor, 1/8 to 1/2 step modes
STEPS PER REVOLUTIONS
400
STEPPING (max./min.)
0.21 µm to 0.850 µm
MOTOR TO DRIVE SCREW RATIO
15/28
15/28
SPEED (max./min.)
5.1 cm/min / 0.0042 cm/hr
18.36964 cm/min / 0.008409
cm/hr
PUMPING RATES
1699 mL/hr with 60 mL syringe,
to 0.73 µL/hr with 1 mL syringe
6120 mL/hr with 60 mL syringe,
to 1.459 mL/hr with 1 mL syringe
MAXIMUM FORCE
35 lb at min. speed, 18 lb at
maximum speed
100 lb at minimum speed, 18 lb at
maximum speed
NUMBER OF PROGRAM PHASES
41
41
RS-232 PUMP NETWORK
100 pumps maximum
100 pumps maximum
POWER supply
Wall adapter 12V DC @ 850 mA
Wall adapter 12V DC @ 1000 mA
DIMENSIONS
22.9 x 14.6 x 11.4 cm
(8.75 x 5.75 x 4.5 in.)
22.9 x 14.6 x 11.4 cm
(8.75 x 5.75 x 4.5 in.)
WEIGHT
1.6 kg (3.6 lb)
1.6 kg (3.6 lb)
1
200
Need a pump for two syringes? Two Aladdin pumps when daisychained are more efficient and affordable than any competitor’s
dual syringe models. Two Aladdins (Al-2000) will perform as a dual
infusion/withdrawal pump, a double pump for infusing at different
rates, a push/pull pump with one infusing and one withdrawing at
the same or different rates, two independent pumps, or a master/
slave pump. One Aladdin can even control the second for continuous
pumping with optional check valve set.
The Aladdin pump series will accept syringes from Becton
Dickinson, Monoject, Terumo, and Air-Tite.
AL-1000
Programmable Syringe Pump AL-1000HP Programmable Syringe Pump, High Pressure
AL-2000
Two AL1000 Syringe Pumps Includes GN-TTL Interconnecting Cable for push/pull or
continuous pumping. Valves not included.
Specify line voltage
When ordering 220V models, specify UK,
Euro or Australian line cord.
OPTIONAL ACCESSORIES
GN-PC7
PC to pump cable, 7 ft
GN-PC25
PC to pump cable, 25 ft
GN-NET7
Pump-to-pump network cable, 7 ft
GN-NET25 Pump-to-pump network cable, 25 ft GN-TTL
Pump-to-pump reciprocating cable
ADPT2
Footswitch
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
177
PUMPS, MICROINJECTION
● Economical
Soon to be your favorite lab tool
Standard and autoclavable pipetters
Single-Channel Pipetters
LM-504247
Volume
Range
µL
Increment
µL
Measurement
Volume
µL
2.5
2.50
20
0.1 - 2.5
0.05
1.25
3.00
3.00
0.25
12.00
6.00
10
1.00
0.80
5
1.50
1.50
1
2.50
1.50
20
0.90
0.40
10
1.20
1.00
LM-504248
LM-504249
0.5 - 10
0.1
LM-504250
LM-504251
2 - 20
0.5
LM-504252
PUMPS, MICROINJECTION
LM-504253
10 - 100
1
LM-504254
LM-504255
20 - 200
1
LM-504256
LM-504257
100 - 1000
5
LM-504258
LM-504259
LM-504260
1000 5000
50
Accuracy
±%
Reproducibility
±%
2
3.00
2.00
100
0.80
0.15
50
1.00
0.40
10
3.00
1.50
200
0.60
0.15
100
0.80
0.30
20
3.00
1.00
1000
0.60
0.20
500
0.70
0.25
100
2.00
0.70
5000
0.50
0.15
2500
0.60
0.30
1000
0.70
0.30
Model Numbers
marked with are
autoclavable
Price
ISO
9001/13485
CERTIFIED
Eight-Channel Pipetters
LM-504261
Model Numbers
marked with are
autoclavable
Volume
Range
µL
Increment
µL
0.5 - 10
0.1
LM-504262
LM-504263
5 - 50
0.5
LM-504264
LM-504265
50 - 300
5
LM-504266
Measurement
Volume
µL
Accuracy
±%
Reproducibility
±%
10
1.00
0.80
5
1.50
1.50
1
2.50
1.50
50
1.00
0.50
25
1.50
1.00
5
3.00
2.00
300
0.70
0.25
150
1.00
0.50
50
1.50
0.80
Price
Twelve-Channel Pipetters
LM-504267
Volume
Range
µL
Increment
µL
0.5 - 10
0.1
LM-504268
LM-504269
5 - 50
0.5
LM-504270
LM-504271
50 - 300
LM-504272
178
5
Measurement
Volume
µL
Accuracy
±%
Reproducibility
±%
10
1.00
0.80
5
1.50
1.50
1
2.50
1.50
50
1.00
0.50
25
1.50
1.00
5
3.00
2.00
300
0.70
0.25
150
1.00
0.50
50
1.50
0.80
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Price
Also available in sets!
Select your choice of 5, 6, or
7 single-channel pipetters
— in either standard or
autoclavable models.
PUMPS, MICROINJECTION
AUTOCLAVABLE SETS
504339 Set of 5 Autoclavable Single-Channel Pipetters
504338 Set of 6 Autoclavable Single-Channel Pipetters
504337 Set of 7 Autoclavable Single-Channel Pipetters
Select from:
LM-504248LM-504250LM-504252
LM-504254LM-504256LM-504258
LM-504260
OPTIONAL STAND (not included with sets)
504592 Stand for LM Series Pipetters (holds 8)
STANDARD SETS
504334 Set of 5 Standard Single-Channel Pipetters
504335 Set of 6 Standard Single-Channel Pipetters
504336 Set of 7 Standard Single-Channel Pipetters
Select from:
LM-504247LM-504249LM-504251
LM-504253LM-504255LM-504257
LM-504259
OPTIONAL STAND (not included with sets)
504592 Stand for LM Series Pipetters (holds 8)
Universal Tips
See page 181
ClickPet Repeating Pipetters
Part Number
Volume
(µL)
# of Dispenses
Fill Volume
(µL)
1-5
1µL - 50
5µL - 10
~50
5-25
5µL - 45
25µL - 9
~200
CP-100
25-100
25µL - 40
100µL - 10
~1000
CP-250
50-250
50µL - 20
250µL - 4
~1150
CP-5
CP-25
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
179
REGAL
pipetters
l Lightweight and conformable
ergonomic design
PUMPS, MICROINJECTION
l Easy calibration using provided tool
l Made from biologically inactive and
chemical inert polymers
l Easy for cleaning and parts replacement
l CE and ISO13485 Certified
Model
Volume
Range
µL
Increment
µL
Nominal
Volume
µL
Tolerance
%
Repeatability
%
REG2
0.2 ~ 2
0.01
0.2
0.5
2
±12.0
±5.0
±2.0
≤6.00
≤2.50
≤0.70
REG10
1 ~ 10
0.1
1
5
10
±3.0
±1.5
±1.0
≤1.50
≤0.60
≤0.40
REG20
2 ~ 20
0.1
2
10
20
±3.0
±1.0
±1.0
≤1.50
≤0.50
≤0.30
REG50
5 ~ 50
0.5
5
20
50
±2.0
±1.2
±1.0
≤1.50
≤0.40
≤0.20
REG100
10 ~ 100
1
10
50
100
±2.0
±0.8
±0.8
≤0.50
≤0.30
≤0.15
REG200
20 ~ 200
1
20
100
200
±2.0
±0.8
±0.8
≤0.50
≤0.30
≤0.15
5
100
500
1000
±1.5
±0.8
±0.8
≤0.30
≤0.30
≤0.15
50
1000
2000
5000
±1.0
±0.7
±0.7
≤0.50
≤0.25
≤0.15
100
1 mL
5 mL
10 mL
±3.0
±0.7
±0.7
≤0.30
≤0.20
≤0.15
REG1000 100 ~ 1000
REG5K
1000 ~ 5000
REG10K 1000 ~ 10000
180
Price
GERS5
GERS6
GERS7
504591
Regal Pipetters (set of any 5) & stand Regal Pipetters (set of any 6) & stand Regal Pipetters (set of any 7) & stand Stand for Regal Pipetters (holds 8) World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Universal Pipette Tips
Ultra-clear and certified RNase/DNase-free
                  Universal Tips
Tip Volume
For Pipetter
REG2
REG10
REG20
0.1 - 10 µL
s—
and
Bulk
Part No.
Rack
Part No.
Bag of 1000
500191
960
(10 racks of 96)
500192
Bag of 1000
500193
960
(10 racks of 96)
500194
1000
(10 racks of 100)
500196
100-1000 µL
REG1K
LM-504257/8
Bag of 1000
500195
500 - 5000 µL
REG5K
LM-504259/60
Bag of 250
500197 *
Bag of 1000
504588
1000 - 10,000 µL REG10K
500
500198 *
(10 racks of 50)
250
(10 racks of 25)
504589
PUMPS, MICROINJECTION
alf
h
t
ea
u
m
o
a
S
b rice!
a
at e p
th
ing
ad
s le
REG20
REG50 LM-504253/4/5/6
REG100 LM-504263/4
REG200
5 - 200 µL
br
LM-504247/8/9
LM-504250/1/2
LM-504261/2
LM-504267/8
                  Universal Filter Tips (sterile)
Tip Volume
0.1 - 10 µL
10 - 200 µL
100-1000 µL
For Pipetter
REG2
REG10
REG20
Bulk
Part No.
LM-504247/8/9
LM-504250/1/2
LM-504261/2
LM-504267/8
REG20
REG50 LM-504253/4/5/6
REG100
LM-504263/4
REG200
REG1K
LM-504257/8
1000 - 10,000 µL REG10K
Rack
Part No.
960 (10 racks of 96)
500199
960 (10 racks of 96)
500200
1000 (10 racks of 100)
500201
250 (10 racks of 25)
504590
                  Universal Gel Loader Tips (flexible)
Tip Volume
For Pipetter
Bulk
Part No.
Rack
Part No.
0.1 - 10 µL (Flat)
0.17 mm OD
REG2
REG10
REG20
LM-504247/8/9
LM-504250/1/2
LM-504261/2
LM-504267/8
200
500202
0.1 - 10 µL (Flat)
0.37 mm OD
REG2
REG10
REG20
LM-504247/8/9
LM-504250/1/2
LM-504261/2
LM-504267/8
200
500203
0.1 - 10 µL
(Round)
0.57 mm OD
REG2
REG10
REG20
LM-504247/8/9
LM-504250/1/2
LM-504261/2
LM-504267/8
200
500204
200
500206
200
500207
0.5 - 200 µL
(Round)
0.57 mm OD
REG20
REG50 LM-504253/4/5/6
Bag of 1000 500205
REG100 LM-504263/4
REG200
REG20
0.5 - 200 µL (Flat) REG50 LM-504253/4/5/6
0.37 mm OD REG100 LM-504263/4
REG200
WPI’s Universal Pipette Tips are for use with Regal and most other pipetters, including Gilson, Oxford Benchmate, Socorex, and SealPette.
* Tips 500197 and 500198 fit Regal, Eppendorf, and BioHit pipetters.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
181
UltraMicroPump III
PUMPS, MICROINJECTION
Three-prong
syringe holder for
more stability
Micro syringes are easily installed —
just snap the barrel into the clamps.
UMP3 accepts a range of syringes
from 0.5 µL to 1 mL.
Manipulator
not included.
Now with
microstepping
Controller now has higher
resolution and is virtually pulse-free
This versatile injector uses microsyringes to deliver picoliter volumes
Perfect for a wide range of applications
including intracellular injection, micro
delivery of biochemical agents or dyes, cell
separation, and in vitro fertilization.
See “Reproducible and Efficient Murine CNS Gene Delivery
Using a Microprocessor Controlled Injector,” A.I. Brooks et
al., Journal of Neuroscience Methods, 80 (1998) 137-147.
ULTRAMICROPUMP SPECIFICATIONS
(based on 10 µL syringe)
NORMAL MODE
TOTAL # OF STEPS
20,000 (63 mm travel)
MINIMUM DISPENSING VOLUME
0.58 nL / step (10 µL syringe)
LINEAR MOTION PER STEP
3.175 microns
WEIGHT
325 g (11.5 oz)
MOUNTING ROD DIAMETERS
7.9 mm (0.31 in.)
Mains power supply 90-264VAC @ 47-63Hz
DIMENSIONS
∅ 32 mm x 190 mm
(∅ 1.3 in. x 7.5 in.)
MICROSTEPPING MODE
Precision is increased eight-fold
182
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
The world’s leading ultramicropump!
With its digital con­trol­ler, UltraMicroPump III can dispense as little
as 600 picoliters per in­cre­men­tal ad­vance of the syringe piston (us­ing a
5 µL syringe). Sy­ringes may be filled externally and then in­sert­ed into the
pump or filled while mount­ed in the pump. Fluids injected or withdrawn
are held en­tire­ly with­in the micro syringe to maintain a low fluid dead
vol­ume.
For po­si­tion­ing, the
UltraMicroPump III
may be attached to any
of sev­er­al WPI mi­cro­
po­si­tion­ers such as
the M3301 (man­u­al),
DC3001 (mo­tor­ized), or
any manual stereotaxic
manipulator.
An Integral component in the UMPIII system is a microprocessor-based
con­trol­ler, SYS-Mi­cro4, which provides an “in­tel­li­gent” and easy-to-use
interface to up to four sy­ringe pumps. Operating pa­ram­e­ters are set with
the mem­brane key­pad and LCD display. From the key­pad the user can
select the following func­tions: set pump to in­fu­sion or with­draw­al mode,
enter the vol­ume to be in­fused or with­drawn, rate of de­liv­ery, and syringe
type as well as syn­chro­nize the start­ing and stopping of any com­bi­na­tion
of sy­ringe pumps.
User parameters can be stored in the device’s “non-vol­a­tile” mem­o­ry for
in­stant re­call when the unit is powered on.
An optional footswitch can be plugged into a connector on the rear of
the controller for “hands free” start‑/‑stop operation.
Computer Control—An RS-232 port on the rear of the controller can be
used to con­nect it to a computer for use with computer control programs.
UMPIII ACCEPTS: glass syringes with barrel diameters from 5.5 to 9 mm.
UMP3-1
UltraMicroPump III (one) and Micro4 Controller
UMP3-2
UltraMicroPump III (two) and Micro4 Controller
UMP3-3
UltraMicroPump III (three) and Micro4 Controller
UMP3-4
UltraMicroPump III (four) and Micro4 Controller
UMP3
UltraMicroPump III (without controller)
SYS-MICRO4 Micro4 Controller, Four-Channel
OPTIONS AND ACCESSORIES
15867
Footswitch for Micro4
40500
RS-232 Cable, 9-pin “D” connector
502201
V-clamp for Stereotaxic Frame
503301
Extension Cable, miniDIN (male-female) 8 ft
Microvolume Syringes
Syringes with Luer Fitting (no needle)
WPI P/N
Volume
Description
ILS005LT 5 µL
ILS 5 µL Gas-tight Luer tip
ILS010LT 10 µL
ILS 10 µL Gas-tight Luer tip
ILS025LT 25 µL
ILS 25 µL Gas-tight Luer tip
SGE050TLL 50 µL
SGE 50 µL Gas-tight Teflon Luer Lock
SGE100TLL 100 µL
SGE 100 µL Gas-tight Teflon Luer Lock
SGE250TLL 250 µL
SGE 250 µL Gas-tight Teflon Luer Lock
O.D. UMP3UMP2UMP1
6.5 mm
Y
Y
N
6.5 mm
Y
Y
N
8.0 mm
Y
Y
N
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
N
N
Syringes with Replaceable Beveled Needles
WPI P/N
Volume Description
SGE0005RN* 0.5 µL
SGE 0.5 µL 23 ga (0.63 mm) 70 mm long
SGE001RN*1.0 µL
SGE 1.0 µL 26 ga (0.47 mm) 70 mm long
SGE005RN 5 µL
SGE 5 µL 23 ga (0.63 mm) 50 mm long
SGE010RNS10 µL
SGE 10 µL 26 ga (0.47 mm) 50 mm long
SGE025RN 25 µL
SGE 25 µL 25 ga (0.50 mm) 50 mm long
SGE050RN 50 µL
SGE 50 µL 25 ga (0.50 mm) 50 mm long
SGE100RN 100 µL
SGE 100 µL 25 ga (0.50 mm) 50 mm long
O.D. UMP3UMP2UMP1
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
8.0 mm
Y
Y
Y
* The plunger extends to the tip of the needle, displacing the full sample during injection - which gives the syringe zero dead volume.
The barrel length of this syringe is 17 cm long vs. the usual 8-9 cm.
SGE and ILS are respective trademarks of Scientific Glass Engineering and Innovative Labor Systeme.
Replacement Needles
RN0005
For syringe SGE0005RN, 23 ga (0.63 mm) 70 mm long
RN001
For syringe SGE001RN, 26 ga (0.47 mm) 70 mm long
RN005
For syringe SGE005RN, 23 ga (0.63 mm) 50 mm long
RN010
For syringe SGE010RN(S), 26 ga (0.47 mm) 50 mm long, 5-pack
RN025
For syringes SGE025RN, SGE050RN, SGE0100RN, 26 ga (0.47 mm) 50 mm long, 5-pack
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
183
PUMPS, MICROINJECTION
UMPIII shown
mounted to
stereotaxic frame
(not included).
Smart Controller
Pneumatic PicoPumps
Repeatable microinjection in volumes ranging from picoliters to nanoliters
Designed to simplify intracellular injection and a variety of other
micro­in­jec­tion tasks, WPI's PicoPumps use carefully regulated air pres­
sures for se­cur­ing cells and injecting them with fluid. Injected volumes
range from picoliters to nanoliters. Separate ports supply positive and
negative pressure—pos­i­tive pressure for high-pressure ejection, and suction
for supporting the cell or for filling the pipette from the tip. A second
pressure port maintains a low positive “holding” pres­sure to the injecting
pipette between injection pulses, to prevent fluid up­take through capillary
action or diffusion. Timing, ejection pressure, hold­ing pressure, and suction
are adjusted inde­pen­dent­ly by control knobs and in­di­ca­tor gaug­es on the
front panel. Injection pressure is controlled by a 20-turn regulator on the
front panel. A built-in timing circuit allows precise control of the amount of
time that the injection pressure is applied to the output port. Time intervals
can range from 10 seconds down to 10 ms or less, depending on the eject
pressure setting. The injection pressure interval can be triggered manually
on the front panel, by footswitch, or by computer controlled TTL pulse. A
5‑volt mon­i­tor output provides a logic-level pulse for your computer or
other mon­i­tor­ing device.
PUMPS, MICROINJECTION
The most recognized picopump in the world!
For a complete list of pre-pulled micropipettes, see µTips™, or call us with your special requirements.
PV830 — Eject pressure, Hold pressure, and Vacuum are all available,
controlled by separate reg­u­la­tors on the front panel. Eject pressure
supplies a high-pressure pulse for injecting fluid. Hold pressure, which
is not sufficient to cause fluid ejection, is used to prevent back filling of
the pipette by capillary action or diffusion when the solenoid is inactive.
Pressure in the injection pipette is au­to­mat­i­cal­ly switched between Eject
and Hold pressure by a precision timing circuit that controls a solenoid
valve. Vacuum is used to fill pi­pettes from the tip or to secure a floating
cell during microinjection. Vacuum is regulated the same way, by a
20-turn knob on the front panel. Vacuum may be switched from regulated
vacuum to at­mo­sphere by using the pneu­mat­ic toggle on the front panel.
Vacuum can also be routed to the eject port.
Each PicoPump is supplied with two PicoNozzle Kits (5430-ALL) plus tubing to connect the
holders to the pressure and vacuum ports.
SYS-PV820 PicoPump w/ hold pressure
SYS-PV830 PicoPump w/ hold pressure and vacuum
Specify line voltage All PicoPumps require external vacuum source — see below.
New PicoNozzle Kit 5430-ALL (included)
allows micropipettes to be securely mount­ed
in micropositioners for stable axial air
delivery. Because air enters the pipette
axially, lateral whipping during injection is
eliminated.
OPTIONAL ACCESSORIES
3260
Foot Switch
2932
Rack Mount Kit, 31⁄ 2-in. high (PV820)
2933
Rack Mount Kit, 51⁄4-in. high (PV830)
5430-10
PicoNozzle Kit (MPH6S for 1.0 mm pipette & 5-ft tubing assembly)
5430-12
PicoNozzle Kit (MPH6S for 1.2 mm pipette & 5-ft tubing assembly)
5430-15
PicoNozzle Kit (MPH6S for 1.5 mm pipette & 5-ft tubing assembly)
5430-20
PicoNozzle Kit (MPH6S for 2.0 mm pipette & 5-ft tubing assembly)
5430-ALL PicoNozzle Kit (for 1.0, 1.2, 1.5, and 1.65 mm pipettes & 5-ft tubing assembly)
75122-110 Replacement Gaskets for PicoNozzle — 1.0 mm, green, package of 10
75122-210 Replacement Gaskets for PicoNozzle — 1.2 mm, black, package of 10
75122-310 Replacement Gaskets for PicoNozzle — 1.5 mm, blue, package of 10
75122-410 Replacement Gaskets for PicoNozzle — 1.65 mm, red, package of 10
MPH6S
Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm)
MPH6R
Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm)
3316
Replacement Input Kit
See NanoFil™ — page 188
184
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
PV820 offers separate regulated Hold and Ejection pres­sure, used to
maintain a low pressure in the pipette between injections to pre­vent unwanted
fluid uptake by capillary action or dif­fu­sion. A pre­ci­sion timing circuit switches
from Eject pressure to Hold pres­sure au­to­mat­i­cal­ly, once timing has been set.
Al­though reg­u­lat­ed vac­u­um is not pro­vid­ed in this mod­el, suction can be pro­
vid­ed by con­nect­ing a vac­u­um source to the vac­u­um port on the rear pan­el.
Suc­tion is then available through the pres­sure ports.
PICOPUMP SPECIFICATIONS
PRESSURE
PV820
PV830
0 to 150 psi
0.3 to 90 psi
12 in. water
10 ms to 10 s in Timed Mode
0.1% (20-turn dial) *
0.05 psi *
3% at full scale *
Quick Connect (1⁄4 in. OD Tubing)
Barbed (1⁄16-in. ID Tubing)
Solenoid
* Both Hold and Eject Pressures
PUMPS, MICROINJECTION
Pressure Input
0 to 150 psi
Pressure Output
0.3 to 90 psi *
Lowest Regulated Pressure
12 in. water *
Pulse Width (10-turn dial)
10 ms to 10 s in Timed Mode
Regulator Accuracy
0.1% (20-turn dial) *
Regulator Repeatability
0.05 psi *
Gauge Accuracy
3% at full scale *
Input Connector
Quick Connect (1⁄4 in. OD Tubing)
Output Connector
Barbed (1⁄16-in. ID Tubing)
ControlSolenoid
* Both Hold and Eject Pressures VACUUM
Vacuum Input
0 to 30.0 in. Hg
0 to 30.0 in. Hg
Vacuum Output
Unregulated
0.2 to 29.9 in. Hg
Lowest Regulated Vacuum
Unregulated
3 in. water
Regulator Accuracy
Unregulated
0.1% (20-turn dial)
Regulator Repeatability
Unregulated
0.03 in. Hg
Gauge Accuracy
None
3% at full scale
Quick Connect (1⁄4 in. OD Tubing)
Input Connector
Quick Connect (1⁄4 in. OD Tubing)
Barbed (1⁄16 in. ID Tubing)
Output Connector
Barbed (1⁄16 in. ID Tubing)
ControlManual
Manual
Vent AtmosphereAtmosphere
CONNECTIONS INCLUDED
Input Kit
Output Kit
10-ft nylon tubing (0.25-in. OD, 1000 psi), one 1⁄2-inch female NPT adapter
Two PicoNozzle assemblies, each consisting of one MPH6S pipette holder, 60-in. of PVC tubing (200 psi), and a luer-fitted aluminum handle.
PHYSICAL SPECIFICATIONS
Power
Dimensions
Shipping Weight
95-135 V or 220-240 V, 50/60 Hz
17 x 3.5 x 9.5 in. (43 x 9 x 24 cm)
11 lb (5 kg)
95-135 V or 220-240 V, 50/60 Hz
17 x 5.25 x 9.5 in. (43 x 13 x 24 cm)
14 lb (6.3 kg)
Miniature Vacuum Pump
●●
●●
●●
●●
●●
●●
●●
MINI VAC Specifications
Power Source
Motor Type
Power
Free Flow
At -100 mbar
Maximum Pressure
Maximum Vacuum
Pump Head Construction
Diaphragm
Valves
Dimensions
Weight
Oil free
Maintenance free
Minimal vibration
Low noise
Extremely long life time
Compact (18 x 7 x 7cm)
Durable aluminum exterior
This miniature vacuum pump is durable and accurate, ideal for any application
requiring a small, reliable pump that provides vacuum pressure up to 250 mbar. The
industrial-strength aluminum exterior, neoprene diaphragm and neoprene/silicone
valves ensure this pump will stand up to daily use.
801566
801963
230 (50 Hz)
Vibrating
4.0 W
4.0 L/min.
2.0 L/min.
–
–250 mbar
120 (60 Hz)
4.0 W
3.0 L/min.
1.5 L/min.
–
–250 mbar
Aluminum
CR-neoprene
CR-neoprene/FPM (Viton)/Silicone
185 x 72 x 72 mm
850 g
Mini Vacuum Pump (110V)
Mini Vacuum Pump (220V)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
185
Nanoliter Injector
For oocyte injection and applications in the 2 to 70 nanoliter range
PUMPS, MICROINJECTION
NANOLITER 2010
WPI’s microprocessor-controlled
Nanoliter 2010 uses direct pis­ton
dis­place­ment. By either push­ing the
injection button on the control box or
press­ing on the optional footswitch,
a calibrated volume will be smooth­
ly injected. The pro­cess is quiet and
vibra­tion free. Capillary filling and
injection speeds are 23 nL/sec and
46 nL/sec (emptying speeds are 92
nL/sec and 230 nL/sec). Maximum
flu­id ejec­tion is 5 µL. Each unit
comes with suffi­cient glass to pull at
least 300 tips. Glass is 1.14 mm O.D.
(nominal) and 0.5 mm I.D.
By setting the DIP switch, the
injection vol­ume can be changed
from 2.3 to 69.0 nL in 16 steps. Up
to 100 injections may be triggered
per filling. Since the volume of a
normal Xenopus oo­cyte is about 500
nL, the instrument has the capability
to inject from less than 1% to over
10% of the total volume of the
oocyte in one preset step increment.
Included: 1 vial 3.5 in. capillaries
(300); replacement “O” rings; Allen
wrench; MicroFilTM MF34G backfilling
needle; and two sample µTip™ prepulled mi­cropi­pettes.
Smart Controller: Mi­cro4, an optional microprocessor-based con­trol­ler,
can provide an “in­tel­li­gent” and easy-to-use interface to up to four Nanoliter
Injectors. Operating pa­ram­e­ters are set with the mem­brane key­pad and LCD
display. From the key­pad the user can set pump to in­fu­sion or with­draw­al
mode, enter the vol­ume to be in­fused or with­drawn, and rate of de­liv­ery,
as well as syn­chro­nize the start­ing and stopping of a
com­bi­na­tion of Injectors. User parameters
can be stored in the device’s
“non-vol­a­tile” mem­o­ry
for in­stant re­call when
the unit is powered on.
An optional footswitch
can be plugged into a
connector on the rear of
the controller for “hands
free” start/stop operation.
An RS-232 port on
the rear of the controller
can be used to con­nect it
to a computer for use with
computer control pro­grams.
Optional smart controller
for Nanoliter 2010
Micromanipulator not included.
NANOLITER 2010
SPECIFICATIONS
INJECTION VOLUME
Variable
REMOTE CONTROL
Yes
GLASS OD
1.14 mm
GLASS ID
0.5 mm
STEP
12.7 µ/step
INJECTION SPEED
Slow
23 nL/sec
Fast
46 nL/sec
FILL SPEED
Slow
23 nL/sec
Fast
46 nL/sec
EMPTY SPEED
92 nL/sec
VARIABLE VOLUME RANGE
2.3 - 69.0 nL
SMALLEST VOLUME
2.3 nL
TO CHANGE VOLUME
Set switch
INJECTIONS PER FILLING, MAX. 100 injections
SHIPPING WEIGHT
3 lb. (1.1 kg)
186
WPI rec­om­mends the following equipment for a complete sys­tem,: PZMIII ste­reo mi­c ro­scope • MMJ joystick
mi­c ro­ma­n­ip­u­la­tor • M10 magnetic base • µTip™ mi­c ropi­pettes.
NANOLITER2010 Nanoliter 2010
NL2010MC4Nanoliter Injector & Micro4 Controller (small controller not included)
OPTIONAL ACCESSORIES
13142
Footswitch for Nanoliter 2010
15867
Footswitch for Micro4 Controller
4878
Replacement 3.5-in. glass capillaries (300)
4879
Replacement 7-in. glass capillaries (300)
TIP10XV119
Micropipettes for Nanoliter Injector (10)
SYS-MICRO4
Micro4 Controller, Four-Channel
300033
Adapter for Micro4
300521Replacement O-rings (five)
500778
Replacement Nanoliter Injector Universal Adapter
500299
Pistons, 5-pack
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Manual Microsyringe Pump
The MMP and DMP are convenient tools for
precise manual injection of fluid using glass pipettes
or similar injection devices. The design allows
visual feedback of flow at the pipette tip. They can
also be used as a manual micro syringe pump for
perfusion or withdrawal of liquids. The resolution
of the injection volume can be continuously
varied from 10 nanoliters to the microliter range,
depending on the syringe used. Either oil or air
can be used as the transfer media to assist the
injection of fluid. The DMP comes with an exclusive
digital micrometer that will allow the reading of
piston advancement easily with a 0.001-millimeter
resolution. The optional software and cable kit can
transmit advancement data directly into computer.
Model MMP has the traditional mechanical micrometer head with a resolution
of 10 microns per division and advances 500 micrometers per revolution. The
entire frame body of the injector is constructed with polished stainless steel for
25 mm
Advances Resolution
0.001 mm for DMP and 0.01 mm for MMP
Syringe Size
10 µL to 1 mL gas tight luer tip syringe
Tubing
1.5 m of PTFE tubing with 0.5 mm ID
Pipette Holder
0.24" x 5.2"
Pipette Holder Fits
1.0 to 1.5 mm OD pipette
excellent stability and durability. The piston of the micrometer can be slid across
the rail to the syringe's plunger position. Small diameter PTFE tubing is used to
improve the accuracy and solution compatibility. The unique design of the pipette
holder can securely hold any pipette with an outer diameter of between 1.0 mm
and 1.5 mm. All necessary accessories for removing air and filling the syringe
and tubing with liquid are included. The system comes complete with a 100 µL
gas tight syringe and other syringe sizes can be purchased.
MMP
Manual Microsyringe Pump
DMP
Manual Microsyringe Pump with Digital Display
Accessories
MMP-KIT
Injection Assembly Parts Kit
Not including valve—see #14057-10, page 131)
Pressure Manometer
For measuring hydrostatic pressures
SYS-PM01D Pressure Manometer (1 psi)
Hand-held and battery
SYS-PM01R Pressure Manometer (1 psi), Rechargeable*
operated, PM Series pressure SYS-PM015D Pressure Manometer (15 psi)
manometers monitor vac­u­um SYS-PM015R Pressure Manometer (15 psi), Rechargeable*
and pressure in nonaqueous
SYS-PM100D Pressure Manometer (100 psi)
fluids. An integral transducer
SYS-PM100R Pressure Manometer (100 psi), Rechargeable*
and dig­i­tal display allow
CBL102
Mini-Phone-to-BNC Cable
easy and accurate pressure
Specify line voltage
readings. Three ver­sions
*Rechargeable
versions
come
with nickel/cadmium battery and charger
measure pressures in the
range of ±1
psi, ±15
PRESSURE MANOMETER SPECIFICATIONS
psi or ±100
PM01
PM015
PM100
psi. A range
PRESSURE RANGES
±1 psi (±52 mm Hg)
±15 psi (±775 mm Hg) ±100 psi (±690 kPa)
switch allows
MAX. PRESSURE
20 psi (1035 mm Hg) 30 psi (1550 mm Hg)
150 psi (1035 kPa)
measurement
RESOLUTION
0.001 psi (0.1 mm Hg) 0.01 psi (1 mm Hg)
0.1 psi (1 kPa)
in units of psi or
OUTPUT
1 V/psi 100 mV/psi 10 mV/psi
kPa for the 100
OUTPUT RANGE
±1.0 V
±1.5 V
±1.0 V
psi version, and psi or
LINEARITY
0.5% full-scale
mmHg for the 15 psi version. Pres­sure
TEMPERATURE EFFECT
1.0% full-scale (0-70°C)
can be read on the built-in LCD display or relayed
ZEROScrewdriver-adjust
to a chart recorder, os­cil­lo­scope, or computer.
RESPONSE TIME
30 ms
POWER
Nine-volt battery
PM Series pressure manometers come with 4 feet
BATTERY LIFE
Alkaline, 200 hours; rechargeable, 25 hours
of 1⁄8-inch ID soft vinyl tub­ing. A mini-phone-to-BNC
RECORDER OUTPUT
Mini-phone jack, 0.141 inch (3.5 mm)
cable for the recorder output is also available (Part
OUTPUT IMPEDANCE
1 kΩ
#CBL102). Standard versions are equipped with a
PNEUMATIC CONNECTORS
Barbed, for 1/8-inch or 3/16-inch ID soft tubing
nine-volt alkaline battery.
DIMENSIONS
SHIPPING WEIGHT
3 x 6 x 1 inches (8 x 15 x 4 cm)
3 lb (1.4 kg)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
187
PUMPS, MICROINJECTION
DMP & MMP Specifications
Travel Distance
solid stainless
steel frame
NanoFil™
Sub-microliter injection system for small animal research
ferrule: ø 8 mm
glass barrel: ø 7 mm
PUMPS, MICROINJECTION
l The world's smallest dead volume injection syringe
l Comes with various needle sizes from 26 ga. to 36 ga.
l Versatile research applications — RPE and IO Kits
l Custom needle shapes available — blunt, sharp, beveled
l Compatible with WPI's UMP3 and PV800 series microinjection systems
NanoFil is a specially designed 10 microliter
syringe developed in response to customer
requests for improved microinjection in mice
and other small animals. It makes quantitative
nanoliter injection much easier and more
accurate than any other method currently in use.
NanoFil's low dead volume eliminates the need
for oil backfilling, a messy process which risks
contamination of the injected sample.Injection
is now simpler, and less messy, and there is
no possibility of oil contamination in critical
applications such as ophthalmology research
(see the Retinal Pigment Epithelial (RPE) and Intra
Ocular (IO) injection kits listed below).
When the inner tip diameter of a conventional
syringe is reduced to less than 100 micron,
it is very difficult to backfill the solution at a
reasonable speed. NanoFil solves this problem
by using a tip coupling mechanism that makes
it possible to change the syringe tip during the
experiment. Simply load the sample using a
larger tip, such as the 26 gauge needle provided
with the syringe, and then replace it with a micro
tip for sample injection. On a conventional 10
microliter syringe, a solid ring or bushing is
permanently bonded to the tubing. Replacing the
tip in middle of the experiment is not practical.
With the NanoFil, tips can be exchanged by a
simple twist of the brass lock, gently pulling out
the tip, and replacing with the desired new tip.
To secure the tip, NanoFil uses an olive shaped
silicon gasket that is similar to, but much sturdier
than, some of the microelectrode holders used
for electro physiology recording. The silicone
gasket makes it possible to hold not only metal
tips but also glass and quartz tubing. Many
types of tubing can be easily connected to the
syringe as long as the outer diameter (OD) is
close to, but not more than, the inner diameter
(ID) of the inside barrel. Flexible quartz capillaries
used in Gas Chromotography (GC) and Capillary
Electrophoresis (CE) can also be easily coupled to
the syringe.
Specially designed tips as small as 36 gauge
(110 micron OD) are offered in both blunt and
beveled styles. Our studies have shown that
these tips will cause less trauma to the tissue
than any other form of micro syringe currently in
use. NanoFil has a unique coupling mechanism
that allows many different forms of small tubing
and tips to be coupled with the syringe barrel.
NANOFIL
NanoFil Syringe, 10 microliter
NANOFIL-100
NanoFil Syringe, 100 microliter
NanoFil syringe does not contain any injection tips, those must be purchased separately. It
does include a 26 gauge beveled needle for backfilling.
REPLACEMENT BACKFILL NEEDLES
NF26BV-2
26G Beveled Needle, 460 µm nominal diameter (package of 2)
Selecting the correct tip for your application
Using NanoFil in different configurations
Direct injection by hand: This is the simplest and most
economical way to inject. Any of our tips can be inserted directly
into the NanoFil syringe. Even the SilFlex tubing can be inserted to
switch from hand injection to the other methods listed below. The
limitation of this method is the difficulty achieving sub microliter
resolution.
Installed on WPI’s UMP-III microsyringe pump: This will allow
the user to achieve nanoliter resolution and reproducibility. For
neural system injection, mount the UMPII on a stereotaxic frame.
SilFlex tubing and holder: The needle is mounted on a small
plastic holder that is connected to the NanoFil by a 35 cm length
of flexible tubing. The NanoFil is mounted on the UMP II pump.
This configuration allows the user to hold the animal in one hand
and insert the needle with the other. When the needle reaches
the desired location, activate the pump using the footswitch and
the pre-programmed injection volume will be delivered. This
configuration gives a nanoliter level of accuracy and reproducibility.
It is best suited for applications such as the RPE and IO injection.
188
The replaceable needles used with the NanoFil are available with either
blunt or beveled tips. The blunt tip is used for injection into soft tissue and
when a uniform solution distribution is needed. The beveled style is used
for applications that involve the penetration of a tough tissue.
One of the main factors that limit the resolution and accuracy of
conventional micro syringes to the upper tens of nanoliters range is
diffusion in the large tip ID. When the tip ID is equal or larger than 100
micron, the error caused by tip diffusion is in the nanoliter range level
((100 micron)^3 = 1 nanoliter). With a 36 gauge needle installed on the
NanoFil, the error caused by diffusion will be reduced to the sub nanoliter
level, making accurate injection of a nanoliter possible.
All of WPI’s beveled tips have a unique 25 degree tri-surface bevel that
is optimized for microinjection. A 10 degree single-surface beveled tip
penetrates better than one with a 25 degree angle, however the distance
between the upper opening to the tip (see section “F” in Fig. 2) is longer.
As a result, it requires a deeper penetration of the tip to achieve the same
level of liquid delivery. Deeper penetration means more tissue damage.
WPI’s unique 25 degree beveled tip solves this problem with two extra
beveled surfaces. The tip of a single surface beveled tip is actually a
blade instead of a point. It dulls very quickly. In contrast, the tri-surfaced
tip has a real point. It not only penetrates much better but is also much
more durable. Our tests show that our 33 gauge, 25 degree beveled tip
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
“E”
“D”
“C”
“A”
“F”
A
“B”
Tip
Tip Order Tip O.D. Tip I.D. Length
Number
“A”
“B”
“C”
Total
Length
“D”
Shank
O.D.
“E”
Bevel
Total
Length Dead
“F”
Volume
Tip
Material
NF33BV-2
210 μm
115 μm
10.0 mm
40.0 mm
460 μm
≈348 µm
1.47 nL
Stainless Steel
NF34BV-2
185 μm
85 μm
5.0 mm
35.0 mm
460 μm
≈290 µm
1.39 nL
Stainless Steel
NF35BV-2
135 μm
55 μm
5.0 mm
35.0 mm
460 μm
≈204 µm
1.38 nL
Stainless Steel
NF36BV-2
110 μm
35 μm
3.0 mm
33.0 mm
460 μm
≈156 µm
1.37 nL
Stainless Steel
NF33FBV-2
200 μm
100 μm
5.0 mm
35.0 mm
460 μm
≈322 µm
1.40 nL
Titanium Alloy
NFQ34-5
160 μm
100 μm
55.0 mm
75.0 mm
460 μm
≈280 µm
0.59 nL
Quartz
NF33BL-2
210 µm
115 µm
10.0 mm
34.0 mm
460 µm
≈348 µm
1.47 nL
Stainless Steel
NF34BL-2
185 µm
85 µm
5.0 mm
29.0 mm
460 µm
≈290 µm
1.39 nL
Stainless Steel
NF35BL-2
135 µm
55 µm
5.0 mm
29.0 mm
460 µm
≈204 µm
1.38 nL
Stainless Steel
NF36BL-2
110 µm
35 µm
3.0 mm
27.0 mm
460 µm
≈156 µm
1.37 nL
Stainless Steel
NF33FBl-2
200 µm
100 µm
5.0 mm
29.0 mm
460 µm
≈322 µm
1.40 nL
Titanium Alloy
Above dimensions apply to blunt tips also.
to limit its length to 2.5 to 3.0 mm and still
maintain a usable strength. Since the tip ID is
in the 25 to 50 micron range, it is very easily
clogged. Therefore, only well filtered solutions
can be used. Depending on the viscosity of the
sample, the user might also need to pre-load
the syringe with a regular tip before switching to
this tip for injection. We recommend using the
35 gauge tip instead of the 36 gauge unless it is
absolutely necessary.
Flexifil: The Flexifil tip is made of a titanium
alloy. The advantage of this tubing is its
durability. This “semi-flexible” tip can be bent up
to 90 degrees without damage. It is also much
more corrosion resistant than the stainless steel
tip. Saline solutions left in the tip will be less
likely to clog it. Although this tip is specified
as a 33 gauge tip, its outer diameter is slightly
smaller than our 33 gauge stainless steel tip.
Flexible Quartz Tubing : The flexible quartz
tubing tip is made of 160 micron OD polyimide
coated quartz tubing with a special adapter
sleeve mounted at the end. It is designed for
filling glass capillary electrodes or pipettes, just
like WPI’s traditional MF34G Microfil. However,
unlike the traditional MicroFil, which has about
50 microliters of dead volume in its luer hub,
the dead volume of this tip is less than 0.6
microliters. It is useful for loading electrodes
with solutions that have a limited volume or are
too expensive to waste.
NANOFIL NEEDLEs
NF33BL-2
33 G blunt NanoFil needle (pkg of 2)
NF34BL-2
34 G blunt NanoFil needle (pkg of 2)
NF35BL-2
35 G blunt NanoFil needle (pkg of 2)
NF36BL-2
36 G blunt NanoFil needle (pkg of 2)
NF33BV-2
33 G beveled NanoFil needle (pkg of 2)
NF34BV-2
34 G beveled NanoFil needle (pkg of 2)
NF35BV-2
35 G beveled NanoFil needle (pkg of 2)
NF36BV-2
36 G beveled NanoFil needle (pkg of 2)
NF33FBL-2
33 G Flexifil blunt NanoFil needle (pkg of 2)
NF33FBV-2
33 G Flexifil beveled NanoFil needle (pkg of 2)
NF33-36BL
Assortment of 4 blunt NanoFil needles
NF33-36BV
Assortment of 4 beveled NanoFil needles
REPLACEMENT PARTS & ACCESSORIEs
NFINHLD
NanoFil Injection Holder
SILFLEX-2
SilFlex tubing 35 cm long (pkg of 2) (dead volume = 2.74 µL)
NFGSK-5
Spare Silicone Gasket for NanoFil & Holder (pkg of 5)
NFQ34-5
34 Gauge Flexible Quartz Tubing for filling (pkg 5)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
189
PUMPS, MICROINJECTION
penetrates easier and lasts longer than other
manufacturer’s 33 gauge, 10 degree single
beveled tips. With a 35 gauge tri-surface
beveled tip, the resistance to the penetration
becomes even less. Each of our tips undergo a
penetration test before leaving the factory to
guarantee the best results for our customers.
Available Tips
33 gauge: This tip is similar to Hamilton’s
7762 and 7803 series removable needles in
both tip length and outer diameter. However,
our beveled tip version is shorter, more durable,
and penetrates better due to the special trisurface grinding technique. In the past, 33
gauge tips were the smallest size sold by other
manufacturers and were frequently cited in
literature. However, our new 35 gauge tip
is much better for injections involving small
animals, especially mice. Compared with
Hamilton’ 33 gauge, 10 degree beveled tip, our
35 gauge 25 degree beveled tip can reduce
the depth of penetration by almost 80%. The
distance between the tip and the upper rim
of the opening (section F on Figure 2) is 348
microns for the 33 gauge tip. The distance
for our 35 gauge tip is only 230 microns.
In addition, the smaller tip size significantly
reduces the required penetration force. In nearly
all applications, a 33 gauge tip can be replaced
with our 35 gauge tip and produce better
results.
34 gauge: This is a transitional size between
the 33 gauge and 35 gauge. If the 35 gauge
is too weak and the 33 gauge is too large, this
makes a good alternative.
35 gauge: This was the most popular and
preferred tip of most scientists during our field
trial. The combination of its strength, length,
durability, and clogging resistance creates a
balance with very little compromising of the
individual properties. It is much smaller than the
33 gauge tip offered by other manufacturers. It
is only slightly larger than the 36 gauge tip but
is much stronger and less likely to be clogged.
Samples can be directly loaded with this tip. Its
5 mm length is sufficient enough for almost all
injection applications in mice.
36 gauge: This is the smallest tip that is
commercially available. The tip is so small that
it can be inserted into the opening of the 33
gauge needle tip. Because this is pushing the
limits of what current technology can produce,
there are some limitations to consider before
using. Its thin diameter makes it necessary
DETAIL A
NanoFil
Application Kits
PUMPS, MICROINJECTION
RPE-KIT
These kits are specially designed for eye research for injecting retinal
pigment epithelium (RPE) and intraocular (IO) in addition to brain injection
in mice. They need to be used with a NanoFil syringe and UMP3 to
achieve accurate, repetitive, and oil free injection in the submicroliter
range. Each kit includes two pieces of Silflex tubing (one for a spare), a
holder assembly, spare gaskets, and an assortment of four tips - blunt for
the RPE kit and beveled tips for the IO kit. Each kit comes with one each of
33, 34, 35 and 36 gauge tips so that first time users can find the best size
for their application.
The Silflex tubing is the most critical component of the kit. This 35
cm long, flexible tubing has a very precise outer diameter for airtight
fitting with the syringe. It also has a small inner diameter for minimum
dead volume, and is very durable when handled correctly. The SilFlex
190
The optional UMP3 stand in the photo below includes the small base (503084), openside clamp (14073-4) and 25cm rod (503070).
is coupled to the injection tip with a mechanism similar to that of the
NanoFil. The dead volume of the entire kit (including the tubing) is less
than 3 microliters. All of the components in the kit are constructed of inert,
solvent resistant, and autoclavable materials for easy cleaning after viral
injection.
Recommended Accessories
RPE-KIT
Retinal Pigment Epithelium (RPE) injection kit (SilFlex
tubing, gasket, holder, and blunt tip mix)
IO-KIT
Intraocular (IO) injection kit (SilFlex tubing, holder,
gasket, and beveled tipmix)
503207
Stand & Clamps
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Non-Pulsatile Microfluidics Pump
●● Non-pulsatile–No moving parts
●● 100nL to 90µL volume range
●● 250nL/min.–10µL/min. flow rate
range
●● Accuracy–Smooth, controlled flow
for a wide range of rates with a
standard deviation of less than 5%
●● Minimal power requirements
– <250mW needed allows this
pump to run off battery or solar
power
●● Rated at 30 PSI, can be modified
for pressure up to 300 PSI
●● Nominal dead volume with the
SF-MFP
self priming cap
The SF-MFP pump delivers smooth, pulse-free flow of volumes from
100nL to 90µL or at rates between 250nL/min. to 10µL/min. Made of
PEEK, this tiny pump is compaible with a range of solvents, solutons,
buffers and reagents.
This pump uses low voltage or current to drive a pumping fluid across a
selective membrane. This, in turn, causes an elastic diaphragm to expand
and push a controlled amount of fluid to your microfluidic application. The
result is very precise and pulse-free flow using a pump that is compatible
with a wide range of biological and chemical reagents and solvents.
Magnetic Quick Connect Joints
Throw out your nut and ferrule connections. Now a proprietary connection
system uses powerful magnets for leak-proof, self aligning connections
that are a snap to assemble. The instantaneous, simple, leak-proof and
solid quick connect system is designed for use with the SF-MFP pump.
Controller
The easy to program controller offers great flexibility. You can choose
set flow or discreet volumetric dispensing. Operate up to eight pumps
independently or together using the controller.
Rapid Response Time — Typical flow profile for switching between forward
and reverse flow using feedback from an external flow sensor. Note the
near instantaneous change in direction and the smooth, nearly error-free
maintenance of flow rates at ±2.5 μL/min.
SF-MFP
Microfluidic Pump & Controller
SF-QC100 QuickConnect
SF-QC101 QuickConnect for 1/16-in. OD tubing
SF-QC102 QuickConnect with 1/4-28 external thread
SF-QC103 QuickConnect with three SF-QC102 fittings
SF-QC104 QuickConnect with four SF-QC102 fittings
SF-QC105 QuickConnect Luer Lock with one SF-QC102 fitting
SF-QCTUBING Tubing for SF-QC101 and SF-QC102 fittings (10 ft)
SF-QC100
SF-QC101
SF-QC102
SF-QC103
SF-QC104
SF-QC105
SF-MFP SPECIFICATIONS
Operational Modes..................Controlled low rate or volumetric dispensing
Flow Rate Range.......................250 nL/min to 10 μL/min
Flow Accuracy...........................Within 5% of setpoint
Flow Precision............................Better than 5% CV (Model 190ES <1% CV)
Dispensing Range.....................100 nL to 90 μL
Dispensing Accuracy...............Within 5% of setpoint
Dispensing Precision................Better than 5% CV (Model 190ES <1% CV)
Capacity......................................90 μL
Dimensions................................Pump: 2.5 x 3.8 x 3.1 cm (1.0 x 1.5 x 1.25 in.)
Controller: 4.0 x 10.0 x 14.0 cm (1.6 x 3.9 x 5.5 in.)
Weight.........................................Pump: 40 g (~1.4 oz)
Controller: 210 g (~7.1 oz)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
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191
PUMPS, MICROINJECTION
●● Compact – Less than 2 in2 footprint
●● Available in a variety of shapes
Optical Detection Systems
Bio Photometric Detection System
2
spectrophotometer
Absorbance @ 260nm [AU]
SPEC­TROS­CO­PY
Choose the wavelength data you want to see. In
many nutrient, water purity and process applications,
full spectrum analysis is not required. With LEDspec
you can eliminate the extraneous data and focus on
those wavelengths you need to see. You can conduct
flow analysis and single-scan applications with high
precision over a large dynamic range.
●● Affordable spectroscopy
●● Dynamic range and baseline noise outperform
CCD and photodiode array based spectrometers
●● LED light source eliminates costly replacement
lamps
●● Integrated reference channel eliminates signal drift
●● Full computer control
●● Integrated math functions allow for baseline
correction at a second wavelength, signal ratio
and more
●● Simplified display with just the data you want to
view
R 2 = 0.9981
1.5
LEDspec
2 µL sample volume
R 2 = 0.9927
1
0.5
0
0
100
200
300
400
500
600
700
DNA [µg/mL]
DNA Calibration Curve using WPI's V-Vette combined with a LEDspecUV
and pharmaceutical compliant spectrophotometer.
LEDspec
l Measures visible wavelengths
l Sample cells: LWCC, Fiber Optic Cuvette Holders, V-Vette
l Wavelength range (nm): 400, 450, 540, 560, 600, 650, 700,
custom
●● Your choice of three wavelengths included
l Applications include:
Two models are available: 2-channel and 4-channel.
LEDspec-2 (2-channel) comes with your choice
of three LED modules (wavelengths). LEDspec-4
(4-channel) also includes your choice of three LED
modules, however, up to four additional wavelengths
can be added, if desired.
Environmental/Oceanography
Nitrite/Nitrate at 540nm
Phosphate at 700nm
Iron at 560nm
Pharmaceutical
Process Control
Semiconductors
Water purity, trace metal analysis (Fe, Pd, Cu, U)
192
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
800
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Data You Want to See
Many biochemistry applications require
information at specific, important wavelengths,
instead of a full spectrum analysis. For example,
the Bradford, BCA and Lowry assays for protein
analysis rely on specific wavelengths.
LEDspec is ideally suited for oceanographic
applications such as detecting nM concentrations of nitrite/nitrate,
phosphate and iron using WPI’s LWCC sample cells. Two or four
independent channel FIA detection systems can be assembled
using a LEDspec-2 or LEDspec-4, respectively.
LEDspec and LEDspecUV are stand-alone LED-based biophotometric detection systems designed to give you the
information you want to see. Now you can conduct flow analysis
and single-scan applications with high precision and a large
dynamic range.
LEDSPEC Specifications
OPTICAL BASICS
LED-based multiple wavelength detector
with build-in reference channel
CHANNELS
2 or 4
DETECTOR Photodiode
SPECTRAL BANDWIDTH (FWHM)
10 nm (LEDs >400nm)
4 nm (260, 280, 340nm LEDs)
DYNAMIC RANGE
0-3 AU
LEDspec can be equipped with up to 7 LEDs of different
wavelengths.
DETECTOR RESOLUTION
24 Bit
NOISE (PEAK TO PEAK)
< 0.1 mAU
Its noise (< 0.1 mAU peak to peak) and drift performance (<0.5
mAU/h) exceeds that of a CCD or photodiode array detection
system at a fraction of the cost.
WARMUP TIME
Instant
FIBER OPTIC INPUT
600 μm
DRIFT
< 0.5 mAU/h
DIGITAL INPUTS AND OUTPUTS
8/8
LEDspec uses dual–beams to reduce light source drift.
Conventional single beam spectrometers notice baseline drift
caused by warm up, temperature stability and bulb aging. A
reference channel in the LEDspec corrects for baseline while you
ANALOG OUTPUT +/- 10 V, scaleable output
DIMENSIONS (W*H*D)
290 x 80 x 250 mm (11.4” x 3.2” x 9.9”)
WEIGHT
2 kg (2.2 lbs)
make sample measurements.
MAINS
INTERFACEUSB
100 – 240 V / 50 - 60 Hz
Data Collection and Analysis
SPEC­TROS­CO­PY
Now, you can analyze output data with LEDspec's easy-to-use
software and export chromatographs directly to your PC (via USB)
in Microsoft® Excel format. Software provides:
l Full computer control of LEDspec
l Continuous flow or single-shot analysis of up to four
independent channels simultaneously or sequentially.
l Immediate calibration and analysis (mean and standard
deviation) of up to four channels
LEDSpec-2
LEDSpec-4
89273
89272
89274
89245
89246
89247
89248
89275
89276
89249
PERIPRO-4LS
MINISTAR
LEDspec biophotometric detection system (VIS), 2 channel, 3 LED modules (choose when ordering)
LEDspec biophotometric detection system (VIS), 4 channel, 3 LED modules (choose when ordering)
LED module, 260 nm
LED module, 280 nm
LED module, 340 nm
LED module, 400 nm
LED module, 450 nm
LED module, 540 nm
LED module, 560 nm
LED module, 600 nm
LED module, 650 nm
LED module, 700 nm
Peri-Star Pro, 4-channel, low rate, small tubing (see page 166)
Miniature Peristaltic Pump, 1-channel (see page 168)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
193
Optical Detection Systems
UltraPath™
A unique multiple long pathlength sample cell for
absorbance spectroscopy
Appearance of instruments presently in these systems differ from those pictured here.
UltraPath™ is a unique high-performance spectrophotometer system
offering user-selectable optical path lengths of 2, 10, 50 and 200 cm.
The instrument operates in the wavelength range of 250 to 730 (UPUV)
or 380 to 730 nm (UPVIS) and has an exceptional dynamic range.
Designed for the detection of low absorbing species in aqueous solutions,
UltraPath is an ideal tool for any study requiring precise and highly
sensitive spectroscopic determination of analytes, either in the lab or in
the field.
Background
substances to the cell wall. In particular, the design greatly minimizes the
problems commonly found with flow cells of long optical pathlengths:
the risk of trapping dust particles, fibers or particulate matter inside the
cell. The UltraPath system includes a low noise photodiode array-based
spectrometer module (TIDAS I: FWHM = 5 nm, noise <0.2 mAU) and a
light source (D4H with UPUV; FO6000 with UPVIS) to measure sample
absorption. Light is coupled from the light source to the sample cell
and from the sample cell to the detector via two fused silica fibers. A
peristaltic pump (PeriPro-4LS) is utilized to draw the sample into the
UltraPath sample cell.
UltraPath was developed by WPI under a collaborative agreement with
NASA (Stennis Space Center) for the spectroscopic determination of
colored dissolved organic matter (CDOM) in seawater and fresh water
environments. It can be used in the laboratory and in the field (i.e., at sea).
CDOM concentrations vary significantly between open ocean samples
with low CDOM (e.g., 0.007 m-1 at 380 nm), and high CDOM freshwater
environments (e.g., 10-20 m-1 at 380 nm). To address these problems the
design requirements of UltraPath mandated the development of a rugged
portable system capable of high sensitivity measurements across a wide
dynamic range. The UltraPath system meets these stringent design criteria
and enables reliable measurement of CDOM in the range of 0.002 m-1 to
200 m-1 (250 to 730 nm).
35
Absorption [1/m]
SPEC­TROS­CO­PY
l Process Control & Oceanography l Rugged system for laboratory and
onboard measuring l Portable & easy to use l User-selected optical path
lengths: 2, 10, 50 & 200 cm l Highly sensitive and stable
194
30
Sarasota Bay
25
Pond
20
15
10
5
0
-5
250
Design
UltraPath has four optical pathlengths contained within a single sample
cell (i.e., 2 cm, 10 cm, 50 cm and 200 cm). The pathlengths are userselectable, offering a very high sensitivity and an extended dynamic
range for UV and VIS absorbance measurements. The fluid path of the
sample cell is optimized to produce a laminar flow that is virtually free
of interference from trapped air bubbles and adherence of dissolved
Sarasota Bay & Pond CDOM
350
450
550
650
Fig. 1 — Two typical absorption spectra measured using UltraPath. The
sample labeled “Sarasota Bay” is a CDOM sample with 34 PSU salinity
collected from Sarasota Bay (Nov. 2007), and the sample labeled “Pond” is a
highly concentrated CDOM sample collected from a local pond in Sarasota,
Florida (Nov. 2007).
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
750
Wavelength [nm]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Mayagüez Bay CDOM
Mayagüez Bay
0.07
0.05
“System Analyzes Water Samples at Sea”, NASA Aerospace Technology
Innovation, 2001, 9 (5). http://nctn.hq.nasa.gov/innovation/innovation95/3techtrans2.html
R. L. Miller and E. D’Sa. “Evaluating the influence of CDOM on the remote
sensing signal in the Mississippi River Bight”. In Eos Transactions AGU Ocean
Sciences, 2002. Honolulu, HI, p. 171.
0.03
0.01
-0.01
380
480
580
680
Wavelength [nm]
Fig. 2 — CDOM Sample “Mayagüez Bay” was collected from the high
salinity oligotrophic waters of Mayagüez Bay on the west coast of Puerto
Rico (2001). Data courtesy of NASA Stennis Space Center.
E. D’Sa, R.L. Miller and R. Trzaska. “Aparent Optical Properties in Waters
Influenced by the Mississippi River”, Proceedings of the Seventh Thematic
Conference, Remote Sensing for Marine and Coastal Environments, 2002, 6
pg, Miami, FL.
R. L. Miller, C. Hall, C. Del Castillo, B. McKee and M. Dagg. “Bio-optical
Properties of the Mississippi River Plume and Adjacent Shelf.” ASLO Aquatic
Sciences, Albuquerque, NM, 2001.
R. L. Miller, M. Belz and S. Y. Liu, “Measuring the absorption of CDOM in the
field using a multiple pathlength liquid waveguide system”, Ocean Optics XV,
paper 1308, Monaco, October 2000.
A standard PC or laptop (not included) is connected to the TIDAS E via
a RJ-4S Etherney interface). For spectrometer requirements and software
options, see TIDAS-E.
Ponceau S measured with 200 cm pathlength
0.025
baseline
0.02
Absorbance
Mobility
The system is designed for mobility. The components of the UltraPath
system are designed to function over a broad range of laboratory and
field environments.
0.01
50
25
0
-25
-0.005
400
450
500
550
600
650
700
Wavelength [nm]
Fig. 3 – Ponceau S absorption measured with UltraPath (200 cm cell).
Ponceau S was dissolved in Millipore water.
SPEC­TROS­CO­PY
UltraPath Specifications
Dynamic range
5 μAU/cm to 1 AU/cm 0.002 m-1 to 200 m-1
Wavelength Range
250 nm – 730 nm (UPUV) 380 nm – 730 nm (UPVIS)
Wavelength Resolution (FWHM) 5 nm
Particulate Absorption
Particulate absorption can be measured by the well established
Quantitative Filter Technique (QFT). WPI now offers a fiber optic filter
holder for Glass Fiber Filters (QFT1, page 206) which can be used with
the spectrometer (TIDAS E) and light source (D4H or FO6000) supplied
with the UltraPath. With this accessory, particulate absorption can be
measured on site, avoiding loss of spectral information due to freezing
and shipping particulate samples to a laboratory.
Noise (peak to peak)
< 0.2 mAU
Drift < 1 mAU/h
Optical pathlength
2, 10, 50 & 200 cm (user selectable)
Sample cell inner diameter
2 mm
Cell Volume
10 mL (at 200 cm pathlength)
Sample inlet / outlet1/8”
Fiber input/output
600 μm
Solvent resistance
Most organic and inorganic solvents
Shipping weight
UPUV: 44 lb (20 kg) UPVIS: 33 lb (20 kg)
Reference
R. L. Miller, M. Belz, C. Del Castillo, R. Trzaska,
75
3 nM
0
Two typical absorption spectra recorded with an UltraPath (UPUV) of
a seawater and a fresh water sample collected in November 2007
are shown in Fig. 1. Due to their high absorbance, both samples were
analyzed in the 10 cm pathlength. The CDOM sample labeled Mayagüez
Bay in Fig. 2 is from oligotrophic, low productive waters with high
salinity collected off the west coast of Puerto Rico in the Mayagüez
Bay. Special attention should be drawn to the exceptional sensitivity of
UltraPath enabling detection of CDOM absorption below 0.03 m-1. To
exemplify the performance of the UltraPath in Laboratory Chemistry and
Process Control, Ponceau S absorbance was measured with the 200 cm
pathlength of an UltraPath. Normalizing the Ponceau absorbance graph
to AU/cm, the range of this measurement is 150 μAU with a noise level
below 2 μAU peak to peak. Sub-nanomolar concentration of this dye can
clearly and reliably be detected, which is a novelty in absorbance based
spectroscopy.
V. Kitidis, A. P. Stubbins, G. Uher, R. C. Upstill
Goddard, C. S. Law, E. M. S. Woodward, “Variability
of chromophoric organic matter in surface waters
of the Atlantic Ocean”, Deep Sea Research Part
II: Topical Studies, Vol. 53, Issue 14-16, 2006, p.
1666-1684.
1.8 nM
0.005
Samples
N. B. Nelson, D. A. Siegel, C. A. Carlson, C.
Swan, W. M. Smethie Jr. and S. Khatiwala. 2007.
Hydrography of chromophoric dissolved organic
matter in the North Atlantic. Deep-Sea Res. I. 54:
710 – 731.
100
0.7 nM
0.015
125
µAU/cm
Absorption [1/m]
0.09
“Determining CDOM Absorption Spectra in Diverse Coastal Environments
Using a Multiple Pathlength, Liquid Core Waveguide System”, Continental Shelf
Research, July 2002, 22:9, p 1301-1310.
UPVIS UPUV Ultrapath System, Visible Light
Ultrapath System, Ultraviolet & Visible Light
The UltraPath system includes: Multiple pathlength cell, Tidas E with TidasDAQ/SpectraView
software, FO-6000 light source (UPVIS) or D2H light source (UPUV), two FO-600-SMA1M optical
fibers, PeriStar Pro peristaltic pump, silicone tubing, sample injector and Waveguide Cleaning Kit.
Specify line voltage
501609
KIT-UPVIS-STARTUP
KIT-UPUV-STARTUP
89575
Waveguide Cleaning Kit
FO-600-SMA1M, 501609, 72100, 800120, 15807
FO-600-SMA1M, 501609, 72100, D2H-DB, D2H-HB, 15807
QFT1, Fiber Optic Holder for Glass Fiber Filters
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
195
Optical Detectors
● Photodiode array
spectrometer module
● Low noise detection
(<0.1 mAU peak to peak)
● Wavelength range
190 nm to 720 nm
● Fiber optic design
Tidas E
SPEC­TROS­CO­PY
High performance fiber optic spectrometer system
WPI’s Tidas E is a high end fiber-optic spectrometer module designed
for low noise applications. The Tidas E outperforms conventional benchbased spectrophotometers and CCD-based spectrometer modules,
when it comes to high precision fiber optic sampling. It relies on a
monolithic optical bench made by Zeiss, which is optimized for fiber
Applications
TIDAS E SPECIFICATIONS
OPTICAL BASICS
Monolithic Spectrometer Module; Concave Aberration
Corrected Holographic Grating; Fiber optic cross section
converter for increased light throughput; 2nd order multilayer filter
DETECTOR ARRAY
Hamamatsu photodiode array, 256 pixel
WAVELENGTH RANGE:
190 - 720 nm
SPECTRAL BANDWIDTH (FWHM)
<10 nm
DETECTOR RESOLUTION
16 Bit
NOISE (PEAK TO PEAK)*
< 0.04 mAU @ 254 nm
WAVELENGTH ACCURACY
<1 nm
WAVELENGTH REPRODUCIBILITY
< 0.1 nm
FIBER OPTIC INPUT
600 μm
SYSTEM REQUIREMENTS
Windows XP, Vista
SOFTWARE (INCLUDED)
TIDASDAQ (data collection) & SpectraView (data analysis)
DIMENSIONS (WxHxD)
260 mm × 150 mm × 140 mm
(10.25" × 5.9" × 5.5")
WEIGHT
2.5 kg (5.5 lb)
INTERFACE
External (RJ-45)
POWER100 - 240 V / 50 - 60 Hz
196
optic applications. Most cuvette-based standard spectrometers lose more
than 90% of light through expensive prism decoupling. The Tidas E
is designed for fiber optic sampling cells. Using suitable light sources
and sample cells, spectral detection in the wavelength range of 190 to
720 nm can be performed at noise levels < 0.04 mAU peak to peak.
The Tidas E is ideally suited for WPI’s fiber optic sampling equipment.
High sensitivity detection systems for flow analysis can be assembled
using WPI’s Liquid Waveguide Capillary Cells (LWCC) with effective
pathlengths ranging from 50 to 500 cm. These setups are frequently used
in fluid injection analysis systems for nutrient analysis (nitrite, nitrate,
phosphate, iron) in oceanographic applications. Microliter sampling
systems for UV/VIS applications can be assembled, using WPI’s V-Vette or
DipTip™ dipping probes (see pages 208–209).
Software
There are separate software packages for data collection and data
analysis for the Tidas E. An instrument driver, TIDASDAQ, is used to run
the spectrometer module, collect spectra in either single or continuous
mode, control the digital I/Os and save the experimental data to disk.
Data analysis is performed with the SpectraView software package.
Further, TIDASDAQ exports data directly into GRAMS/AI, a feature very
useful for advanced data analysis for pharmaceutical applications and
requirements.
TIDAS-I Tidas I Spectrometer Module with TidasDAQ/SpectraView
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
TidasDAQ: Data Collection
and Instrument Control
With TidasDAQ, high precision intensity,
absorbance, transmittance or normalized
spectra can be obtained in less than a second.
Only a few parameters need to be adjusted to
obtain spectral data. Sampling of single scans,
continuous full spectra scans or triggered scans
is possible. Chromatograms can be displayed
and logged to disk at up to four wavelengths.
Data Export of 2D and 3D Spectrograms, as
well as Chromatograms is supported in ASCII,
Spectralys/SpectraView, Excel and Grams/
AI formats. Light sources and other sampling
instrumentation can be controlled via the TTL
level digital outputs, as well as data collection
can be triggered by TTL leveled external inputs
of the TIDAS E.
Figure 1: TIDASDAQ
acquisition window, showing
an absorbance baseline.
SpectraView: Data Analysis
SpectraView is a spectroscopy data analysis
package targeted at flow analysis applications.
It processes data recorded by TIDASDAQ.
SpectraView allows to display recorded spectra
in 2D (default) and 3D view. Mathematical
computation, Derivation, Smoothing,
Quantification and other functions are available
to work with your data. The Quantification
module allows single point and multiple point
analysis, multiple linear regression, partial least
square and principle component analysis. Data
can be exported out of a 3D analysis file into
separate scans. Further, chromatograms as well
as spectrograms can be copied directly into
Excel for further data analysis.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
197
SPEC­TROS­CO­PY
Figure 2: SpectraView 3D view. Spectra can be
displayed and analyzed in 2D and 3D format. This
allows the user to conveniently interpret “time
acquisition” data typically done with a TIDAS-ELWCC flow system
Light Sources
Deuterium halogen light source
with integrated TTL shutter
Replacement Deuterium
Lamp 503847
The D4H is a combined deuterium and halogen light source for UV/VIS
and NIR applications. This light source is ideally suited to work with WPI’s
spectrometer modules and sample cells. It supplies a continuous spectrum
in the UV, VIS and NIR range from 200 nm to 1100 nm. The D4H is
equipped with an integrated electrical shutter, which can be controlled by
a switch or a TTL signal.
LIGHT SOURCE SPECIFICATIONS
200—1100 nm
380—1700 nm
Deuterium lamp life
2000 hr
NA
Tungsten/Halogen lamp life
2000 hr
3000* hr
1-2 mAU/h
<0.5 mAU/h
Stability
Deuterium
Halogen
Deuterium + Halogen
40000
UV/VIS/NIRVIS/NIR
Spectral Range
D4H Spectrum
50000
D4HFO-6000
Application
60000
Intensity [counts]
SPEC­TROS­CO­PY
Replacement Halogen
Lamp 503848
Power Consumption
140 W
6W
Power requirements
110/240V, 50-60Hz, 1A
12VDC/1A
Shutter/TTL triggerYes
Max. Fiber Output
1000 µm
Connections
30000
Shipping Weight
20000
Dimensions (W/H/L) 10000
Yes
1000 µm
SMASMA
13.2 lb (6 kg) 1.3 lb (0.6 kg)
7 x 6.2 x 9.8 in.
(17.8 x 15.7 x 25 cm) 4.8 x 2.8 x 7.5 in.
(12 x 7 x 19 cm)
*Lamp life is dependent upon internal power settings.
0
200
300
400
500
600
700
Wavelength [nm]
D4H
503848
503847
UV
Safety
Gog­gles
Deuterium Halogen Light Source (200 nm-1100 nm)
Halogen replacement lamp
Deuterium replacement lamp ( > 215 nm)
Goggles fit over
regular glasses
13410
198
UV Safety Goggles
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
FO-6000
LED-Lite™
High color temperature
tungsten light source
Modular LED Light source with
exchangeable LEDs
The LED-lite is a power supply for WPI’s ELS LED modules for
monochromatic light excitation. Each ELS module has an SMA bulk head
fitting and allows direct attachment of SMA terminated fibers.
ELS module
Normalized Intensity [cts]
FO-6000-FILT
ELS Spectral Distributions
Color
UV
Blue
Blue
Blue
Blue-Green
Green
Yellow
Orange
Red
λmax
Spectral Line
Half Width
Estimated
Output
370 nm
430 nm
450 nm
470 nm
495 nm
525 nm
590 nm
623 nm
660 nm
12 nm
65 nm
70 nm
20 nm
35 nm
40 nm
13 nm
15 nm
35 nm
85 µW
15 µW
119 µW
140 µW
227 µW
80 µW
60 µW
114 µW
275 µW
Estimated output is after light has passed through a 1 mm fiber.
LED-LITEELS Power Supply (requires ELS module)
Includes transformer and AC adapter.
Specify line voltage
ELS-xxx
ELS-370
300051
300052
The FO-6000-FILT inline filter
holder directly attaches to the
FO-6000 light source. This allows
a virtual light loss free insertion of
optical filters with outer diameters
from 8 to 25.4 mm and thickness
ranging from 2 to 10 mm into the
light path of the FO-6000. With this
filter holder and an optical filter, a
highly stable monochromatic light
source can be assembled.
External Light Source Module (specify wavelength)
ELS Module (370 nm)
Fiber Optic Collimator (SMA)
Fiber Optic Collimator (ST)
To order ELS, use wavelength as suffix to
part number (e.g. ELS-430).
FO-6000
Fiber Optic Light Source
FO-6000FILT Inline Filter Holder Adapter for FO-6000
800120
Replacement Lamp for FO-6000
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
199
SPEC­TROS­CO­PY
This new miniature tungsten
FO-6000 Spectrum
light source has been
developed for high precision
1. 0
portable and low-power
0. 8
spectroscopy applications.
0. 6
Its advantage lies in its
high light power output, its
0. 4
effective color temperature
0. 2
FO-6000
of 6000K and its
2760 K Tungsten Lamp
0. 0
exceptionally low drift below
300 400 500 600 700 800 900 1000
0.5 mAU/h. The FO-6000
Wavelength [nm]
is a light source for the
extended visible part of the
light spectrum (380 nm – 1700 nm). It has a SMA type output connector.
Shutter and lamp can be operated via TTL external triggering. This
light source offers a wide assortment of field applications in analytical
chemistry as well as environmental and life science.
A significant problem with tungsten light bulbs is their inherent low light
output at wavelengths below 430 nm. The FO-6000 was developed
to overcome this limitation. The light intensity of a tungsten light bulb
(2760K) drops below 10% at 420 nm wavelength. However, using
FO-6000, the light intensity drops below 10% at 370 nm, where the
intensity of the conventional tungsten light bulb is at approximately 2%
relative light output. The inherent low noise and low drift of the FO-6000
makes it particularly suitable for
low-noise detection systems.
Flow Cells
See Mini★Star
on page 168
See LEDspec
on page 192
Liquid Waveguide Capillary Cells (LWCC) are
fiber optic cells that combine an increased
optical pathlength (50–500 cm) with small
sample volumes (125–1250 mL). They
can be connected via optical fibers to a
spectrophotometer with fiber optic capabilities.
Ultra-sensitive absorbance measurements can
be performed in the ultraviolet (UV), visible
(VIS) and near-infrared (NIR) to detect low
sample concentrations in a laboratory or
process control environment. According to Beer’s
Law the absorbance signal is proportional to
chemical concentration and light path length.
Compared with a standard 1 cm cell, a 1 mAU
signal is enhanced fifty-fold with a 50 cm cell
to 50 mAU, using WPI’s patented aqueous
waveguide technology*. The LWCC can be
connected directly to a pump or can even be
filled using a syringe. Based on fiber optics, the
LWCC is designed for use with WPI’s LEDspec
photometric detector (see pages 192-193).
LWCC Key Features
Pathlength, internal volume, and wavelength range (measured
with ultrapure water and a Tidas II spectrophotometer
Pathlength [cm]
Internal Volume [µL]
Wavelength Range [nm]
measured with Tidas II
50
100
250
500
125
250
500
1250
230-800
230-730
250-730
280-730
LWCC-3050
LWCC-3100
LWCC-3250
LWCC-3500
Typical Efficiency Curves for LWCC
Percent Transmission (%T)
SPEC­TROS­CO­PY
Liquid Waveguide Capillary Cell
LWCC specifications
50
40
Pathlength
30
50cm
100cm
250cm
20
10
0
200
300
400
500
600
700
800
900
Wavelength (nm)
These spectra show the optimal detection limits for LWCCs of varying pathlength.
200
WAVEGUIDE MATERIAL
Fused silica tubing coated with a low refractive index polymer
OPTICAL PATHLENGTH
50-500 cm
INNER DIAMETER 550 µm
INTERNAL VOLUME
≈ 5 - 1250 µL
SAMPLE INLET/OUTLET COMPRESSION FITTING 1/16”, 1/32”
FIBER INPUT
SMA, ID = 400 µm
MINIMUM PRESSURE*
1.5 - 3 PSI
SOLVENT RESISTANCE
Most organic & inorganic solvents
SHIPPING WEIGHT
1.4 kg (3 lb)
*A one-meter Type II waveguide of 550 µm ID requires about 1.5 PSI
for water flow of 1 mL/min.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Ultra-sensitive Microliter UV/Vis Spectroscopy with 2 to 500 cm optical pathlength!
Mote Marine Laboratories in Sarasota, Florida
has partnered with WPI, using the Company’s
waveguide technology. WPI customized the LWCC
installed in the instrument package inside these
AUV’s (Autonomous Underwater Vehicles). The
LWCCs are used to monitor the color of seawater
in the Gulf of Mexico. One of the primary interest
areas of this research is red tide algae blooms.
Further, modular spectrophotometric sample
systems can be assembled using a TIDAS E
spectrometer and a UV/VIS light source such as
D4H and FO-6000 (see pages 198-199).
Applications
Waveguide Cleaning Kit (#501609), above,
includes the most commonly needed cleaning
solutions for the LWCC waveguides. The LWCC
Start-up Kit (#KITLWCC), at right, includes two
fiber optic cables (#FO-600-SMA1M), Sample
Injector Assembly (#58006), MiniStar™ Peristaltic
Pump, and WaveGuide Cleaning Kit (#501609).
LWCC Injection System
For flow analysis, including simple fluid
injection analysis (FIA) setups, add WPI’s LWCC
injection system (WPI #89372). A selection
valve provides baseline or cleaning solutions
to the sample stream. The injection valve
injects a sample into the stream, avoiding the
introduction of air bubbles or changes of flow
rate.
* Related Patents
Micro Chemical Analysis Employing Flow Through
Detectors, 1995, U.S. Patent No. 5,444,807.
Aqueous Fluid Core Waveguide, 1996, U.S. Patent No.
5,507,447.
Long Capillary Waveguide Raman Cell, 1997, U.S.
Patent No. 5,604,587.
Chemical Sensing Techniques Employing Liquid-Core
Optical Fibers, U.S. Patent No. 6,016,372
LWCC-3050
LWCC-3100
LWCC-3250
LWCC-3500
Liquid Waveguide Capillary Cell, pathlength = 50 cm Liquid Waveguide Capillary Cell, pathlength = 100 cm Liquid Waveguide Capillary Cell, pathlength = 250 cm Liquid Waveguide Capillary Cell, pathlength = 500 cm Accessories
A sample injector assembly can be used to conveniently fill an LWCC with sample solution using a peristaltic pump. Please
note that the LWCC requires two optical fibers to connect to spectrophotometer system. Choose between anti-solarized 400
micron core or UV-enhanced cables (may be ordered in 1 or 3 meter lengths).
89372
LWCC Injection System
58006
Sample Injector Attachment
PERIPRO-4LS Peri-Star™ Pro Peristaltic Pump (see page 182)
MINISTAR
Miniature Peristaltic Pump, 1-channel (see page 184)
FO-600-SMA1M Fiber Optic cable, 1m, SMA, 600 µm core, UV-enhanced
501609
Waveguide Cleaning Kit
KITLWCC
LWCC Start-up Kit*
58450
Kit, Adapter Syringe, LWCC
*includes FO-600-SMA1M (two), 58006, MINISTAR, 501609
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
201
SPEC­TROS­CO­PY
LWCCs have been used in a variety of
applications, such as liquid chromatography,
stopped-flow and colormetric detection,
drinking water analysis, as well as
environmental and oceanographic monitoring
systems (see References on www.wpiinc.com).
WPI’s Liquid Waveguide Capillary Cells are
made of fused silica tubing with an outer
coating of a low refractive index polymer. This
results in high signal stability and easy removal
of air bubbles trapped in the sensor cell due to
the hydrophilic character of the cell wall.
V-Vette
Sample holder for spectroscopic
analysis of microliter volume samples
l 2 microliter sample
l 1 mm pathlength
l No moving parts
l Baseline repeatability < 2 mAU
l Patent pending
V-Vette is a fiber optic sample cell with a pathlength of
1 mm for spectroscopic analysis of microliter volume
samples. Light is coupled into and out of the sample cell via
optical fibers. A 2 μL sample droplet can be conveniently
placed into the v-shaped sample compartment from a
pipetter. Absorbance of the sample is measured between
the input and output fiber after a cover is placed on the
sample compartment to minimize stray light. The sample
can be picked up and reused or removed by blowing it off
with dry air or wiping it off.
V-VETTE
V-VETTE SPECIFICATIONS
Functionality. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Absorbance
Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Included
Pathlength. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 mm
Wavelength Range. . . . . . . . . . . . . . . . . . . . . . . . 200 – 1000 nm
Fiber Connection. . . . . . . . . . . . . . . . . . . . . . . . . . 600 μm (SMA)
Sample Volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 µL
Baseline Repeatability . . . . . . . . . . . . . . . . . . . . . < 2 mAU peak to peak
V-Vette Microliter Sample Holder MicroLWCC
SPEC­TROS­CO­PY
Low volume flow cell for FIA, HPLC
and Process Analysis
MicroLWCC is a new fiber optic low volume flow cell
for UV/VIS/NIR absorbance analysis. Based on WPI’s
established liquid core waveguide technology, the
analyte solution functions as the core of a fluid filled
light waveguide. Wetted parts in the sample cell light
path are PEEK, fused silica and PTFE. Optical fibers are
used to transport light to and from the sample cell. The
cell can be used in biochemistry for DNA, RNA & protein
LWCC-M Specifications
LWCC-M-10 LWCC-M-50LWCC-M-100
Optical pathlength
10 mm
50mm
100mm
Internal volume
2.4 µL
12 µL
24 µL
< 15 mAU
< 30 mAU
Refractive Index @ 280 nm** < 7 mAU
Wavelength Range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200 – 1000 nm
Fiber Connection [µm]. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 500 (SMA)
Transmission @ 254 nm * . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 40%
Maximum Pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . > 1000 psi
Wetted materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . PEEK, Fused Silica, PTFE
* Reference: 2 * 600 µm Fiber, butt-coupled
** Measured using ASTM E 685 - 93
WPI U.S. Patents: 5,444,807; 5,570,447; 5,604,587; 6,603,556; 6,385,380.
202
quantification, colorimetric nutrient and trace metal
analysis, drug discovery and dissolution testing, process
control, and HPLC analysis.
LWCC-M-10
Low Volume Flow Cell, 10 mm pathlength
LWCC-M-50
Low Volume Flow Cell, 50 mm pathlength
LWCC-M-100 Low Volume Flow Cell, 100 mm pathlength
References
M. Belz, “Simple and sensitive protein detection system using UV LEDs
and liquid core waveguides”, Advanced Environmental, Chemical, and
Biological Sensing Technologies V, Optics East, Oct 2007, Proc. SPIE, Vol.
6755, 675505
M. Belz, F. A. Klein, H. S. Eckhardt, K. Klein, D. Dinges, K. T. V. Grattan,
“Optical Detection Techniques and Light Delivery with UV LEDs and
Optical Fibres”, Third International Conference on Optical and Laser
Diagnostics, Proc. IOP, City University, London, UK, May 2007.
M. Belz, P. Dress, A. Sukhitskiy, S. Liu, “Linearity and effective optical
pathlength of liquid waveguide capillary cells”, Part of the SPIE
Conference on Internal Standardization and Calibration; Architectures for
Chemical Sensors, Boston Massachusetts, September 1999, SPIE Vol.
3856, 271-281.
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Dipping Probes
World’s Smallest
Fiber Optic Dipping Probe
for UV/Vis
Spectroscopy
10 mm light pathlength
DipTip™ is a miniature transmission probe for microliter spectroscopic
sampling. DipTip’s tip diameter is only 1.5 mm—the size of a 17-gauge
needle. It will fit into all micro centrifuge tubes on the market and is
a useful tool for measuring protein and DNA samples. It can also be
used for a dissolution system. Because of WPI’s proprietary optical
design, the standard DipTip is not only four to five times smaller in
diameter than any other product on the market, but also costs 50%
less. Now, the new Mini DipTip (with smaller handle and shorter shaft)
is ten times more efficient than the standard DipTip.
New Mini DipTip features
smaller handle, shorter cable,
greater efficiency!
5 mm light pathlength
2 mm light pathlength
Microliter samples can be analyzed cost effectively when you combine
the Mini DipTip with one of the following:
●● The Fiber optic-based spectrometer (Tidas I) and a light source
(D4H and FO-6000)
●● A spectrophotometer (Tidas 100)
●● WPI’s biophotometric detection system (LEDspec)
The Mini DipTip is ideal for multi-channel applications.
●●
10 times more efficient than standard DipTips
Compatible with many standard spectrophotometers (600µm
fiber optic coupler connections)
More flexible cable than standard DipTips
SPEC­TROS­CO­PY
●●
●●
DIPTIP Specifications
DIP-NIR
DIP-UV-SR
DIP-UV-MINI
Tip Diameter
1.5 mm
1.5 mm
1.5 mm
Light Pathlength
2, 5, 10mm
2, 5, 10mm
2, 5, 10mm
Wavelength Range (nm)
350-1000
200-1000
200-1000
Sample Volume Required
20-50 µL
20-50 µL
20-50 µL
Distance From Tip to Upper Edge
of Sample Window
7 mm
7 mm
7 mm
Fiber Length
1.5 m
1.5 m
1.0 m
Fiber Optic Connection
SMA 905
SMA 905
SMA 905
Launch Fibers (2) NA = 0.22
400 µm
400 µm
Return Fiber (1) NA = 0.22
400 µm
400 µm
Launch Fiber Bundle (7 x 200µm)
680 µm*
Return Fiber Bundle (7 x 200µm)
680 µm*
*Circular packaging of the fiber bundle results in an active area equivalent to a fiber with a core diameter of
680 µm. Using a 600 µm connection is recommended and will result in negligible light loss.
DNA samples (with a sample volume of 50µL inside
micro centrifuge PCR vials) were measured in PBS
with concentrations 0–43µg/mL using a Tidas
spectrophotometer.
DIP-NIRx
DipTip™ for VIS/NIR Spectroscopy (2, 5, and 10mm path)
DIP-UV-SRx
DipTip™ for UV/VIS with solar resistant fibers (2, 5, and 10mm path)
DIP-UV-MINI-x Mini DipTip™ for UV/VIS/NIR (2, 5, and 10mm path)
Specify pathlength when ordering: X = 2, 5 or 10
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
203
Optical Glass and Quartz Cuvettes for
● High Quality Glass Cuvettes
● Standard Quartz Cuvettes
● Now offering an expanded line of standard, self-masking, and flow cuvettes!
packaged, glass cuvettes are shipped in
and synthetic quartz cuvettes are shown in
WPI’s glass and synthetic quartz cuvettes
packages of 10 cuvettes. These economic
Fig. 1 (cuvettes were empty, thickness 1.25 mm
are ideal for UV/VIS/NIR absorbance or
quartz and glass cuvettes are ideal for precision
x 2, including surface reflections, measured with
fluorescence experiments.
measurements because of their high quality
a TIDAS II against air as reference).
Synthetic quartz can be used in deep UV
materials used and their low manufacturing
A complete transmission spectrum from
applications and is recommended for
tolerances. Typical transmission curves of glass
190 nm to 4 mm is shown in Fig. 2 (cuvettes
fluorescent applications, as it does not exhibit
were empty, thickness 1.25 mm x
background fluorescence. Quartz
2, including surface reflections).
Technical Characteristics
cuvettes (absorbance, fluorescence
and flow) are shipped individually
Cuvette Material Spectral Range
Transmission Difference ( >80% )
Between Different Cuvettes
Optical Glass
350 – 2500 nm
Less than 1%
Synthetic Quartz
200 – 2500 nm
Less than 1%
SPEC­TROS­CO­PY
Transmission [%]
100
80
60
40
20
Synthetic Quartz
Glass
0
200
400
600
800
1000
Wavelength [nm]
Fig. 1—Transmission curves of Glass and
Synthetic Quartz Cuvettes
Style A
Style B
Style C
Fig. 2—Complete transmission curves of Glass
and Synthetic Quartz Cuvettes
Style D
204
Style E
Style F
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Spectrophotometry and Fluorometry
WPI PN
Style
material
Polished path Dimensions
windows [mm]
[mm]
volume
[mL]
Beam
width
[mm]
Price per cell
standard rectangular cuvettes
CUV2101-1*
CUV2102-1*
B
Quartz
2
1
3.5x12.5x45
0.35
10
B
Quartz
2
2
4.5x12.5x45
0.7
10
CUV2011-1*
B
Quartz
2
5
7.5x12.5x45
1.7
10
CUV1022-10
C
Optical Glass
2
10
12.5x12.5x45
3.5
10
CUV2012-1
CUV2105-1
CUV2106-1
CUV2107-1
CUV2108-1
C
C
C
C
C
Quartz
Quartz
Quartz
Quartz
Quartz
2
2
2
2
2
10
20
30
40
50
12.5x12.5x45
22.5x12.5x45
32.5x12.5x45
42.5x12.5x45
52.5x12.5x45
3.5
7
10.5
14
17.5
10
10
10
10
10
*89341
*89342
*89337
pack of 10
Cuvette spacer for 1-mm cuvettes (part CUV2101-1)
Cuvette spacer for 2-mm cuvettes (part CUV2102-1)
Cuvette spacer for 5-mm cuvettes (part CUV2011-1, CUV2023-1, CUV2063-1)
Self masking Semi micro Cell Cuvette
CUV2023-1*
CUV2031-1
CUV2025-1
CUV2028-1
CUV2032-1
CUV2033-1
CUV2034-1
D
Quartz
2
5
7.5x12.5x45
0.7
4
D
D
D
D
D
D
Quartz
Quartz
Quartz
Quartz
Quartz
Quartz
2
2
2
2
2
2
10
20
50
10
10
10
12.5x12.5x45
22.5x12.5x45
52.5x12.5x45
12.5x12.5x45
12.5x12.5x45
12.5x12.5x45
1.4
2.8
7
1
0.7
0.35
4
4
4
3
2
1
Self masking continuous flowthrough cell
E
Quartz
2
5
7.4x12.5x45
0.035
∅3
E
E
E
F
Quartz
Quartz
Quartz
Quartz
2
2
2
2
10
20
30
10
12.5x12.5x45
22.6x12.5x45
32.6x12.4x45
12.5x12.5x45
0.07
0.14
0.21
0.48
∅3
∅3
∅3
4x12
Self masking continuous flow through cell, small input, large output Z=8.5mm
CUV2614-1
H
Quartz
2
10
12.4x12.4x35.6
0.03
∅2
Micro Cell with black walls
CUV2674-1
J
Quartz
2
10
12.5x12.5x45
0.05
2
Fluorescence
CUV2051-1
CUV2052-1
A
A
Quartz
Quartz
4
4
10
10
12.5x12.5x45
12.5x12.5x45
3.5
1.4
10
4
Long Path Cuvette
CUV2071-1
G
Quartz
2
100
102.5 x 22 ∅
28
19
Style G
Style H
Style J
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
SPEC­TROS­CO­PY
CUV2063-1*
CUV2061-1
CUV2065-1
CUV2066-1
CUV2062-1
US: Tel: 941-371-1003 • [email protected]
205
Accessories
Z-Dimensions Are Not Created Equal
by Lisa J Fulghum
Fiber Optic Collimator
SPEC­TROS­CO­PY
Cuvettes come in a variety of shapes and sizes, but one of the
most important specifications of a cuvette is its Z-dimension. The
Z-dimension of an instrument (cuvette holder or spectrometer) is the
distance from the bottom of the cuvette chamber floor to the center
of its light beam (see image). A cuvette’s Z-dimension must match the
Z-dimension of the instrument with which it will be used.
Each manufacturer designs its instruments with a specific Z-dimension.
Common Z-dimensions include 8.5 and 15mm, and sometimes
20mm. When purchasing small volume cuvettes, the correct
Z-dimension becomes critical. Matching the Z-dimension of the cuvette
to the Z-dimension of the instrument ensures that the light beam
passes through the center of small samples. The table below shows
the standard Z-dimension of the spectrometer sample compartments
for many manufacturers.
ManufacturerZ-Dimension
Agilent® 15 mm
Avantes® 15 mm
Beckman® 8.5 mm
Bio-Rad® 8.5 mm
Cecil® 15 mm
Eppendorf® 8.5 mm
Hewlett – Packard® 15 mm
Hitachi® 8.5 mm
Jasco® 11 mm
J & M® 8.5 mm
Ocean Optics® 15 mm
Perkin – Elmer® 15 mm
Pharmacia® 15 mm
Shimadzu® 15 mm
Spectronics® 8.5 mm
Stellarnet® 15 mm
Turner® 8.5 mm
Varian ® 20 mm
WPI
15mm
WPI’s Fiber Optic Collimator can be used for both collimating a light beam
emitted by an optical fiber or coupling light from a collimated light beam
into an optical fiber. The numerical aperture of the collimator is optimized for
maximum coupling efficiency into typical fused silica fibers. The collimator
can, for example, be used to guide a parallel light beam through a sample
cuvette or an optical filter with virtually no optical losses. In this application,
one collimator collimates the light into a parallel beam 5 mm in diameter,
enabling it to pass a long distance without losing the energy. After the light
passes the sample media, a second collimator can be used to collect the beam
into the receiving fiber. A unique design feature of this collimator is that the
distance between the lens and the optical fiber can be easily adjusted. This
permits it to be used as a focusing device or for fine-tuning the color balance
when coupling light from a light source into a fiber.
COLLIMATOR Specifications
Lens diameter 5 mm
Lens focal distance
10 mm
Lens material
Ultraviolet grade synthetic fused silica (KU-1)
Wavelength range
220 nm-2 µm
Mounting threads
3/8-24 UNF
Divergence
< 0.1 rad for 1 mm core fiber
Fiber connector interface
SMA or ST
300051
300052
OPTIONAL
13395
13370
CC-3-UV
Fiber Optic Collimator (SMA)
Fiber Optic Collimator (ST)
ACCESSORIES
SMA Bulkhead Feedthru connector/coupler, D-hole
SMA half-length Bulkhead coupler/connector
Cosine Corrector
To determine the Z-dimension of a cuvette holder:
l Use strips of heavy paper that will fit neatly into a cuvette (for
example, 12mm x 50mm) and not allow light to pass through the
cuvette.
l Poke a tiny hole in each paper “sample.” For example, one paper
sample could have a hole at 8.5mm, one at 15mm, one at 20mm.
CC-3-UV
13395
13370
l One at a time, insert the paper samples into the cuvette and place
the cuvette into the cuvette holder. The paper sample with the pin
hole at the instrument’s Z-dimension will allow light to pass. The
other paper samples will not allow light to pass.
If you have an instrument that is not on the list and need to
know its Z-dimension, please contact WPI at 941-371-1003 or
[email protected]
206
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Filter Holder
for Glass
Fiber Filters
QFT1
Simple measurements for
particulate absorption
Performance
A significant advantage of the filter holder is its large
beam diameter of 5 mm, resulting in “averaging out” of
larger non-organic particles frequently found on the filter
pad when using natural samples. The removable filter
fixture allows simple filter alternation and cleaning.
WPI’s filter holder for particulate absorption
measurements is specially designed for field
use. It is rugged and portable. It performs as
well as a laboratory based spectrophotometer. It
can be directly connected to WPI’s line of fiber
optic spectrometers and light sources. Instead
of collecting your samples, transporting them to
a laboratory, and accepting the loss of spectral
information associated with it (Sosik, 1999),
particulate absorption can now be measured on site.
Specifications
GF/F Filter Diameter 25 mm
Wavelength Range
280-730 nm *
Fiber Optic Connection ∅ 600 μm / SMA
Material in contact with filter padDelrin
Weight ................. 0.5 kg (1 lb)
How does it work ?
* Using a TIDAS E spectrometer and D4H UV/VIS light source.
Detector and light source requirements
The optical throughput of QFT1 equipped with a classical GF/F filter is very
low and requires a matched light source / spectrometer system. WPI’s TIDAS E
in combination with WPI’s FO-6000 tungsten light source or D4H deuterium/
halogen light source can be used in the 380–730 nm and 280–730 nm
wavelength range, respectively. The QFT1 can also be interfaced to any other
CCD, PDA or scanning type spectrometer with fiber optic capabilities.
In-Line Fiber
Optic Filter Hold­er
References
Mitchell, B. G., “Algorithms for Determining the Absorption Coefficient of
Aquatic Particles Using the Quantitative Filter Technique (QFT)”, SPIE Vol. 1302
Ocean Optics X (1990), 137-148.
Sosik, H. M., “Storage of marine particulate samples for light-absorption
measurements”, Limnol. Oceanogr., 44(4), 1999, 1139-1141
M. Belz, K. Larsen, K.-F. Klein, “Fiber optic sample cells for polychromatic
detection of dissolved and particulate matter in natural waters”, Proc. SPIE, Vol.
6377, Oct 2006, 63770X
89575
QFT1, Fiber Optic Holder for Glass Fiber Filters
This In-Line Fiber Optic Filter Holder allows the insertion of optical
filters within a fiber optic pathway. The connectors of the filter holder
assembly are com­pat­i­ble with WPI’s range of fiber optic jumper cables
and can be coupled using SMA or ST connectors.
Filters with outer diameters from 8 to 25.4 mm and thick­ness­es from
2 to 10 mm can be accomodated. The design lim­its lateral and axial
movement of the filter when secured in the holder.
Two fiber optic collimators are internally mounted in the holder to
pass collimated light through the filter and then refocus the filtered
light into the aperture of the out­put fiber. Spectral range will be largely
limited by the bandpass of the optical fibers (from UV to near IR using
WPI UV-enhanced ca­bles).
56200
In-Line Fiber Optic Filter Holder (SMA)
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
207
SPEC­TROS­CO­PY
Particulate absorption of fresh and seawater can be
determined by filtering a known amount of sample through a Glass
Fiber Filter (GF/F) and measuring the particulate absorption coefficient ap(λ)
concentrated on the filter. This technique is called quantitative filter technique
(QFT) and corrects for the pathlength amplification, an effect of scattering. The
correction of the pathlength amplification and the correction of the non-linear
relationship between the optical density of samples on a Whatman GF/F filter
and in suspension are discussed in Mitchell (1990).
Optical Fibers
Bifurcated Fiber
Optic Assemblies
UV/VIS
Use to combine similar intensity light from
differing sources or to split a light source into
two fibers. For use with a dual spectrometer as
a reference.
BIF22
BIF44
BIF41
BIF62
BIF66
Split or combine similar intensities (200/200)
Split or combine similar intensities (400/400)
Combine UV (400) + VIS (100)
Combine UV (600) + VIS (200)
Split or Combine Similar Intensities (600/600)
WPI can build custom fiber optic assemblies for many
UV/VIS/NIR applications. Call for more information.
Plastic Fiber Optic Cables
More flexible than glass fibers, these
inexpensive PMMA plastic fibers can be used
for illumination and scientific applications. They
are excellent for light transfer between 350 nm
and 1000 nm. Their maximum temperature
should be kept below 80° C.
PLASTIC FIBER OPTIC CABLES (NON UV), 400 TO 1000 NM
FOP1-SMA
Plastic Fiber Optic Cable, SMA connectors, 1 mm x 2 m
FOP1-SMA/ST
Plastic Fiber Optic Cable, ST/SMA connectors, 1 mm x 2 m
FOP1-ST
Plastic Fiber Optic Cable, ST connectors, 1 mm x 2 m
SPEC­TROS­CO­PY
UV-enhanced fiber optic cables
of such a fiber, defined by the 1/e reduction in
transmission at 240 nm, is normally less than 200
minutes. This effect renders them unsuitable for
UV spectroscopy below 240 nm.
Anti-solarization fibers suitable for deep UV
spectroscopy solve this problem. These fibers
stabilize to less than 5% degradation over
a period of 20,000 hours after an initial
transmission “burn-in” loss of less than 25%.
Additionally, this anti-solarization characteristic will
not degrade over time.
Features
● Broad UV/Vis spectral range ● Laser damage resistant ● High core to clad ratios ● Broad
temperature range ● Bio-compatible materials
● Radiation resistant ● Sterilizable by ETO and
gamma radiation ● Higher transmission than PCS
between 180‑nm and 300 nm
Properties
● Multimode • Pure silica core • Numerical aperture: 0.22 ± 0.02 (standard)
● Standard prooftest: 70 kpsi • Minimum bend
radius: 100x clad radius (momentary), 600x
clad radius (long term)
Anti-Solarization
The transmission of conventional UV-enhanced
silica/silica fiber decreases rapidly at wavelengths
below 240 nm when exposed to high intensities
of a deuterium lamp. This effect is called
“UV-solarization” and results from the generation
of color centers in the fiber material. The lifetime
208
Over 20,000 hours
with less than 5%
degradation!
Attenuation
dB/km
1000—
100—
Transmission/m 99%
10—
99.9%
1—
200
300
400
Wavelength, nm
500
600
700
800
900
1000
UV-ENHANCED FIBER OPTIC CABLES, 230 – 1000 NM
FO-50-SMA1M Fiber Optic Cable, 1 m, SMA, 50 µm Core, UV-Enhanced
FO-50-SMA
Fiber Optic Cable, 3 m, SMA, 50 µm Core, UV-Enhanced
FO-100-SMA1M Fiber Optic Cable, 1 m, SMA, 100 µm Core, UV-Enhanced
FO-100-SMA
Fiber Optic Cable, 3 m, SMA, 100 µm Core, UV-Enhanced
FO-200-SMA1M Fiber Optic Cable, 1 m, SMA, 200 µm Core, UV-Enhanced
FO-200-SMA
Fiber Optic Cable, 3 m, SMA, 200 µm Core, UV-Enhanced
FO-400-SMA1M Fiber Optic Cable, 1 m, SMA, 400 µm Core, UV-Enhanced
FO-400-SMA
Fiber Optic Cable, 3 m, SMA, 400 µm Core, UV-Enhanced
FO-400SMA/ST Fiber Optic cable, 1 m, SMA/ST connector, 400 µm core, UV-Enhanced
FO-600-SMA1M Fiber Optic Cable, 1 m, SMA, 600 µm Core, UV-Enhanced
FO-600-SMA
Fiber Optic Cable, 3 m, SMA, 600 µm Core, UV-Enhanced
FO-1000-SMA1M Fiber Optic Cable, 1 m, SMA, 1000 µm Core, UV-Enhanced
FO-1000-SMA
Fiber Optic Cable, 3 m, SMA, 1000 µm Core, UV-Enhanced
ANTI SOLARIZATION FIBER OPTIC CABLES, 190 – 1000 NM
FO-200AS-SMA Fiber Optic Cable, 1 m, SMA, 200 µm Core, Anti-Solarization
FO-400AS-SMA Fiber Optic Cable, 1 m, SMA, 400 µm Core, Anti-Solarization
FO-600AS-SMA Fiber Optic Cable, 1 m, SMA, 600 µm Core, Anti-Solarization
PLASTIC FIBER OPTIC CABLES (NON UV), 400 TO 1000 NM
FOP1-SMA
Plastic Fiber Optic Cable, SMA connectors, 1 mm x 2 m
FOP1-SMA/ST
Plastic Fiber Optic Cable, ST/SMA connectors, 1 mm x 2 m
FOP1-ST
Plastic Fiber Optic Cable, ST connectors, 1 mm x 2 m
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
1100
Product Index
Item
Page
Page
3517 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3531 . . . . . . . . . . . . . . . . . . . . . . . . . 128
3551 . . . . . . . . . . . . . . . . . . . . . . . . . 128
3578 . . . . . . . . . . . . . . . . . . . . . 109, 119
3579-20 . . . . . . . . . . . . . . . . . . . . . . . 19
3669 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3670 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3742-20 . . . . . . . . . . . . . . . . . . . . . . 107
3955 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3960 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3993 . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4878 . . . . . . . . . . . . . . . . . . . . . . . . . 186
4879 . . . . . . . . . . . . . . . . . . . . . . . . . 186
4886 . . . . . . . . . . . . . . . . . . . . . . . . . 100
4898 . . . . . . . . . . . . . . . . . . . . . 100, 101
5052 . . . . . . . . . . . . . . . . . . . . . . . . . 146
5153 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5210 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5233 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5361 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5362 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5371 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5372 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5373 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5374 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5375 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5377 . . . . . . . . . . . . . . . . . . . . 53, 56, 66
5378 . . . . . . . . . . . . . . . . . . . . 53, 56, 66
5385 . . . . . . . . . . . . . . . . . . . . . . . . . 109
5399 . . . . . . . . . . . . . . . . . . . . . . . 54, 66
5430 . . . . . . . . . . . . . . . . . . . . . . . . . 184
5430-12 . . . . . . . . . . . . . . . . . . . . . . 184
5430-15 . . . . . . . . . . . . . . . . . . . . . . 184
5430-20 . . . . . . . . . . . . . . . . . . . . . . 184
5430-ALL. . . . . . . . . . . . . . . . . . . . . . 184
5435 . . . . . . . . . . . . . . . . . . . . . . . 53, 54
5436 . . . . . . . . . . . . . . . . . . . . . . . 53, 54
5440 . . . . . . . . . . . . . . . . . . . . . . . . . 118
5444 . . . . . . . . . . . . . . . . . . . . . . . . . 121
5447 . . . . . . . . . . . . . . . . . . . . . . . . . . 78
5464 . . . . . . . . . . . . . . . . . 137, 140, 143
5468 . . . . . . . . . . . . . . . . . . . 81, 88, 128
5469 . . . . . . . . . . . . . . . . . . . . . . . . . . 88
5470 . . . . . . . . . . . . . . . . . . . . . . . . . . 88
5475 . . . . . . . . . . . . . . . . . . . . . . . . . 155
5479 . . . . . . . . . . . . . . . . . . . . . . . . . 146
5482 . . . . . . . . . . . . . . . . . . . . . . . . . . 88
5483 . . . . . . . . . . . . . . . . . . . . . . . . . . 88
5489 . . . . . . . . . . . . . . . . . . . . . . . . . . 78
6820 . . . . . . . . . . . . . . . . . . . . . 100, 101
7128 . . . . . . . . . . . . . . . . . . . . . 100, 101
7325 . . . . . . . . . . . . . . . . . . . . . . . 53, 54
7326 . . . . . . . . . . . . . . . . . . . . 53, 56, 66
7335 . . . . . . . . . . . . . . . . . . . . . 100, 101
7341 . . . . . . . . . . . . . . . . . . . . . 100, 102
7342 . . . . . . . . . . . . . . . . . . . . . 100, 102
7357 . . . . . . . . . . . . . . . . . . . . . . . . . . 54
7521 . . . . . . . . . . . . . . . . . . . . . . . . . . 54
13024. . . . . . . . . . . . . . . . . . . . . . . . 104
13025. . . . . . . . . . . . . . . . . . . . . . . . 104
13142. . . . . . . . . . . . . . . . 125, 127, 186
13156-100 . . . . . . . . . . . . . . . . . . . . 107
13157-100 . . . . . . . . . . . . . . . . . . . . 107
13158-100 . . . . . . . . . . . . . . . . . . . . 107
13159-100 . . . . . . . . . . . . . . . . . . . . 107
13160-100 . . . . . . . . . . . . . . . . . . . . 107
13161-100 . . . . . . . . . . . . . . . . . . . . 107
13162-100 . . . . . . . . . . . . . . . . . . . . 107
13163-100 . . . . . . . . . . . . . . . . . . . . 107
13316. . . . . . . . . . . . . . . . 100, 101, 118
13324. . . . . . . . . . . . . . . . . . . . . . . . 109
13338. . . . . . . . . . . . . . . . . . . . 151, 155
13347. . . . . . . . . . . . . . . . . . . . . 66, 109
13370. . . . . . . . . . . . . . . . . . . . . . . . 206
13388. . . . . . . . . . . . . . . . . . . . . . . . 109
13395. . . . . . . . . . . . . . . . . . . . . . . . 206
13410. . . . . . . . . . . . . . . . . . . . . . . . 198
13451. . . . . . . . . . . . . . . . . . . . . . . . 109
13555. . . . . . . . . . . . . . . . . . . . . . . . 109
Item
Page
13620. . . . . . . . . . . . . . . . . . . . . . . . 109
13685. . . . . . . . . . . 109, 174, 175, 176
13776. . . . . . . . . . . . . . . . . . . . . . . . 109
13822-10 . . . . . . . . . . . . . . . . . . . . . 107
13834. . . . . . . . . . . . . . . . . . . . . . . . . 131
13854. . . . . . . . . . . . . . . . . . . . . . . . 109
13962. . . . . . . . . . . . . . . . 174, 175, 176
14003. . . . . . . . . . . . . . . . . . . . . . . . . 24
14003-G . . . . . . . . . . . . . . . . . . . . . . . 24
14011. . . . . . . . . . . . . . . . . . . . . . . . 106
14012. . . . . . . . . . . . . . . . . . . . . . . . 106
14034-40 . . . . . . . . . . . . . . . . . . . . . 107
14035-10 . . . . . . . . . . . . . . . . . . . . . 107
14036-15 . . . . . . . . . . . . . . . . . . . . . 107
14038-10 . . . . . . . . . . . . . . . . . . . . . 107
14039-10 . . . . . . . . . . . . . . . . . . . . . 107
14040-50 . . . . . . . . . . . . . . . . . . . . . 107
14041-60 . . . . . . . . . . . . . . . . . . . . . 107
14042-100 . . . . . . . . . . . . . . . . . . . . 107
14043-20 . . . . . . . . . . . . . . . . . . . . . 107
14044-5 . . . . . . . . . . . . . . . . . . . . . . 107
14045-20 . . . . . . . . . . . . . . . . . . . . . 107
14047-10 . . . . . . . . . . . . . . . . . . . . . 107
14048-20 . . . . . . . . . . . . . . . . . . . . . 107
14051-100 . . . . . . . . . . . . . . . . . . . . 107
14054-10 . . . . . . . . . . . . . . . . . . . . . 107
14055-2 . . . . . . . . . . . . . . . . . . . . . . 107
14057-10 . . . . . . . . . . . . . . . . . . . . . 107
14058-10 . . . . . . . . . . . . . . . . . . . . . 107
14059-2 . . . . . . . . . . . . . . . . . . . . . . 107
14061-60 . . . . . . . . . . . . . . . . . . . . . 107
14073-4 . . . . . . . . . . . . . . . . . . . . . . 111
14088. . . . . . . . . . . . . . . . . . . . . . . . 109
14095. . . . . . . . . . . . . . . . . . . . . . . . . 21
14096. . . . . . . . . . . . . . . . . . . . . . . . . 22
14097. . . . . . . . . . . . . . . . . . . . . . . . . 22
14098. . . . . . . . . . . . . . . . . . . . . . . . . 21
14099. . . . . . . . . . . . . . . . . . . . . . . . . 22
14101. . . . . . . . . . . . . . . . . . . . . . . . . 22
14104. . . . . . . . . . . . . . . . . . . . . . . . 135
14106. . . . . . . . . . . . . . . . . . . . . . . . 135
14109. . . . . . . . . . . . . . . . . . . . . . . . . 26
14109-G . . . . . . . . . . . . . . . . . . . . . . . 26
14119. . . . . . . . . . . . . . . . . . . . . . . . . 27
14119-G . . . . . . . . . . . . . . . . . . . . . . . 27
14122. . . . . . . . . . . . . . . . . . . . . . . . . 24
14122-G . . . . . . . . . . . . . . . . . . . . . . . 24
14124-G . . . . . . . . . . . . . . . . . . . . . . . 24
14125. . . . . . . . . . . . . . . . . . . . . . . . . 24
14125-G . . . . . . . . . . . . . . . . . . . . . . . 24
14126. . . . . . . . . . . . . . . . . . . . . . . . . 24
14130. . . . . . . . . . . . . . . . . . . . . . . . . 27
14130-G . . . . . . . . . . . . . . . . . . . . . . . 27
14192. . . . . . . . . . . . . . . . . . . . . . . . . 26
14192-G . . . . . . . . . . . . . . . . . . . . . . . 26
14218. . . . . . . . . . . . . . . . . . . . . . . . . 25
14218-G . . . . . . . . . . . . . . . . . . . . . . . 25
14219. . . . . . . . . . . . . . . . . . . . . . . . . 25
14219-G . . . . . . . . . . . . . . . . . . . . . . . 25
14226. . . . . . . . . . . . . . . . . . . . . . . . . 23
14226-G . . . . . . . . . . . . . . . . . . . . . . . 23
14254. . . . . . . . . . . . . . . . . . . . . . . . 109
14393. . . . . . . . . . . . . . . . . . . . . . . . . 25
14393-G . . . . . . . . . . . . . . . . . . . . . . . 25
14394. . . . . . . . . . . . . . . . . . . . . . . . . 25
14394-G . . . . . . . . . . . . . . . . . . . . . . . 25
14404. . . . . . . . . . . . . . . . . . . . . . . . . 32
14405. . . . . . . . . . . . . . . . . . . . . . . . . 32
15590. . . . . . . . . . . . . . . . . . . . . . . . 160
15623. . . . . . . . . . . 109, 174, 175, 176
15624. . . . . . . . . . . 109, 174, 175, 176
15676. . . . . . . . . . . . . . . . 174, 175, 176
15810. . . . . . . . . . . . . . . . . . . 54, 55, 56
15867. . . . . . . . . . . . . . . . . . . . 183, 186
15873. . . . . . . 136, 137, 140, 142, 143
15914. . . . . . . . . . . . . . . . . . . . . . . . . 23
15914-G . . . . . . . . . . . . . . . . . . . . . . . 23
15915. . . . . . . . . . . . . . . . . . . . . . . . . 23
15915-G . . . . . . . . . . . . . . . . . . . . . . . 23
Item
15920. . . . . . . . . . . . . . . . . . . . . . . . . 23
15920-G . . . . . . . . . . . . . . . . . . . . . . . 23
15921. . . . . . . . . . . . . . . . . . . . . . . . . 23
15921-G . . . . . . . . . . . . . . . . . . . . . . . 23
15922. . . . . . . . . . . . . . . . . . . . . . . . . 25
15922-G . . . . . . . . . . . . . . . . . . . . . . . 25
15923. . . . . . . . . . . . . . . . . . . . . . . . . 25
15923-G . . . . . . . . . . . . . . . . . . . . . . . 25
15934. . . . . . . . . . . . . . . . . . . . . . . . 129
15975. . . . . . . . . . . . . . . . . . . . . . . . 109
15976. . . . . . . . . . . . . . . . . . . . . . . . 109
40500. . . . . . . . . . . . . . . . . . . . . . . . 183
47510. . . . . . . . . . . . . . . . . . . . . . . . . 70
47520. . . . . . . . . . . . . . . . . . . . . . . . . 70
47530. . . . . . . . . . . . . . . . . . . . . . . . . 70
47540. . . . . . . . . . . . . . . . . . . . . . . . . 70
48000. . . . . . . . . . . . . . . . . . . . 124, 129
48014. . . . . . . . . . . . . . . . . . . . . . . . 129
48014-05 . . . . . . . . . . . . . . . . . . . . . 129
48014-10 . . . . . . . . . . . . . . . . . . . . . 129
48014-30 . . . . . . . . . . . . . . . . . . . . . 129
48015. . . . . . . . . . . . . . . . . . . . . . . . 129
48015-10 . . . . . . . . . . . . . . . . . . . . . 129
48015-30 . . . . . . . . . . . . . . . . . . . . . 129
48025. . . . . . . . . . . . . . . . . . . . . . . . 129
48200. . . . . . . . . . . . . . . . . . . . . . . . 129
48300. . . . . . . . . . . . . . . . . . . . . . . . 129
56200. . . . . . . . . . . . . . . . . . . . . . . . 207
58006. . . . . . . . . . . . . . . . . . . . . . . . 201
58450. . . . . . . . . . . . . . . . . . . . . . . . 201
75040. . . . . . . . . . . . . . . . . . . . 125, 127
75050. . . . . . . . . . . . . . . . . . . . 125, 127
75090. . . . . . . . . . . . . . . . . . . . . . . . 125
75122-110 . . . . . . . . . . . . . . . . . . . . 184
75122-210 . . . . . . . . . . . . . . . . . . . . 184
75122-310 . . . . . . . . . . . . . . . . . . . . 184
75122-410 . . . . . . . . . . . . . . . . . . . . 184
77020. . . . . . . . . . . . . . . . . . . . . . . . 115
83016. . . . . . . . . . . . . . . . . . . . . . . . . 99
89245. . . . . . . . . . . . . . . . . . . . . . . . 193
89246. . . . . . . . . . . . . . . . . . . . . . . . 193
89247. . . . . . . . . . . . . . . . . . . . . . . . 193
89248. . . . . . . . . . . . . . . . . . . . . . . . 193
89249. . . . . . . . . . . . . . . . . . . . . . . . 193
89272. . . . . . . . . . . . . . . . . . . . . . . . 193
89273. . . . . . . . . . . . . . . . . . . . . . . . 193
89274. . . . . . . . . . . . . . . . . . . . . . . . 193
89275. . . . . . . . . . . . . . . . . . . . . . . . 193
89276. . . . . . . . . . . . . . . . . . . . . . . . 193
89337. . . . . . . . . . . . . . . . . . . . . . . . 205
89341. . . . . . . . . . . . . . . . . . . . . . . . 205
89342. . . . . . . . . . . . . . . . . . . . . . . . 205
89575. . . . . . . . . . . . . . . . . . . . . . . . 207
91580. . . . . . . . . . . . . . . . . . . . . . 55, 56
91736. . . . . . . . . . . . . . . . . . . . . . . . . 13
91750. . . . . . . . . . . . . . . . . . . . . . . . . 13
100042. . . . . . . . . . . . . . . . . . . . . 53, 56
100084. . . . . . . . . . . . . . . . . . . . . . . . 53
300033. . . . . . . . . . . . . . . . . . . . . . . 186
300040. . . . . . . . . . . . . . . . . . . . . . . 109
300051. . . . . . . . . . . . . . . . . . . 206, 207
300052. . . . . . . . . . . . . . . . . . . 206, 207
300102. . . . . . . . . . . . . . . . . 79, 88, 109
300276. . . . . . . . . . . . . . . . . . . . . . . 160
300305. . . . . . . . . . . . . . . . . . . . . . . . 60
300521. . . . . . . . . . . . . . . . . . . . . . . 186
500028. . . . . . . . . . . . . . . . . . . . . . . 149
500075. . . . . . . . . . . . . . . . . . . . . . . . 28
500076. . . . . . . . . . . . . . . . . . . . . . . . 28
500077. . . . . . . . . . . . . . . . . . . . . . . . 28
500081. . . . . . . . . . . . . . . . . . . . . . . 109
500082. . . . . . . . . . . . . . . . . . . . . . . 109
500085. . . . . . . . . . . . . . . . . . . . . . . . 21
500086. . . . . . . . . . . . . . . . . . . . . . . . 24
500121. . . . . . . . . . . . . . . . . . . . . . . . 32
500128. . . . . . . . . . . . . . . . . . . . . . . 109
500131. . . . . . . . . . . . . . . . . . . . . . . 109
500162. . . . . . . . . . . . . . . . . . . . . . . 154
500184. . . . . . . . . . . . . . . . . . . . . . . 109
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
Page
US: Tel: 941-371-1003 • [email protected]
209
PRODUCT INDEX
1B100-3 . . . . . . . . . . . . . . . . . . 114, 131
1B100-4 . . . . . . . . . . . . . . . . . . 114, 131
1B100-6 . . . . . . . . . . . . . . . . . . 114, 131
1B100F-3. . . . . . . . . . . . . . . . . . 114, 131
1B100F-4. . . . . . . . . . . . . . . . . . 114, 131
1B100F-6. . . . . . . . . . . . . . . . . . 114, 131
1B120 . . . . . . . . . . . . . . . . . . . . 114, 131
1B120-4 . . . . . . . . . . . . . . . . . . 114, 131
1B120-6 . . . . . . . . . . . . . . . . . . 114, 131
1B120F-3. . . . . . . . . . . . . . . . . . 114, 131
1B120F-4. . . . . . . . . . . . . . . . . . 114, 131
1B120F-6. . . . . . . . . . . . . . . . . . 114, 131
1B150-3 . . . . . . . . . . . . . . . . . . 114, 131
1B150-4 . . . . . . . . . . . . . . . . . . 114, 131
1B150-6 . . . . . . . . . . . . . . . . . . 114, 131
1B150F-3. . . . . . . . . . . . . . . . . . 114, 131
1B150F-4. . . . . . . . . . . . . . . . . . 114, 131
1B150F-6. . . . . . . . . . . . . . . . . . 114, 131
1B200-4 . . . . . . . . . . . . . . . . . . 114, 131
1B200-6 . . . . . . . . . . . . . . . . . . 114, 131
1B200F-4. . . . . . . . . . . . . . . . . . 114, 131
1B200F-6. . . . . . . . . . . . . . . . . . 114, 131
2B150F-4. . . . . . . . . . . . . . . . . . . . . . 116
2B150F-6. . . . . . . . . . . . . . . . . . . . . . 116
3B120F-4. . . . . . . . . . . . . . . . . . . . . . 116
3B120F-6. . . . . . . . . . . . . . . . . . . . . . 116
4- or 7-Barrel Pipette Puller. . . . . . . . 130
5B120F-4. . . . . . . . . . . . . . . . . . . . . . 116
5B120F-6. . . . . . . . . . . . . . . . . . . . . . 116
7B100F-4. . . . . . . . . . . . . . . . . . . . . . 116
7B100F-6. . . . . . . . . . . . . . . . . . . . . . 116
7B120F-4. . . . . . . . . . . . . . . . . . . . . . 116
8-Channel Digital Stimulator . . . . . . . . 99
121 . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
260 . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
705 . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
705PF . . . . . . . . . . . . . . . . . . . . . . . . . 73
711P . . . . . . . . . . . . . . . . . . . . . . 81, 128
712P . . . . . . . . . . . . . . . . . . . . . . . 74, 75
715P . . . . . . . . . . . . . . . . . . . . . . . 74, 75
773 . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
900A . . . . . . . . . . . . . . . . . . . . . . . . . . 90
900AP . . . . . . . . . . . . . . . . . . . . . . . . . 90
900APP. . . . . . . . . . . . . . . . . . . . . . . . 90
1300M. . . . . . . . . . . . . . . . . . . . 124, 128
1350M. . . . . . . . . . . . . . . . . . . . . . . . 128
1358 . . . . . . . . . . . . . . . . . . . . . . . . . 109
1571 . . . . . . . . . . . . . . . . . 100, 101, 121
1965 . . . . . . . . . . . . . . . . . . . . . . . . . 116
1966 . . . . . . . . . . . . . . . . . . . . . . . . . 116
1967 . . . . . . . . . . . . . . . . . . . . . . . . . 116
2026-10 . . . . . . . . . . . . . . . . . . . . . . 109
2478 . . . . . . . . . . . . . . . . . . . . . . . . . 128
2479 . . . . . . . . . . . . . . . . . . . . . . . . . 128
2505 . . . . . . . . . . . . . . . . . . . . . . . . . 121
2541 . . . . . . . . . . . . . . . . . . . . . . . . . . 73
2547 . . . . . . . . . . . . . . . . . . . . . . . 72, 75
2851 . . . . . . . . . . . . . . . . . . . . . . . . . 109
2932 . . . . . . . . . . . . . . . . . . . . . 104, 184
2933 . . . . . . . . . . . . . . . . . . . . . 104, 184
2935 . . . . . . . . . . . . . . . . . . . . . . . . . 104
3006 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3161 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3259 . . . . . . . . . . . . . . . . . . . . . . . . . . 93
3260 . . . . . . . . . . . . . . . . . . . . . . . . . 184
3294 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3301 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3302 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3316 . . . . . . . . . . . . . . . . . . . . . . . . . 184
3414 . . . . . . . . . . . . . . . . . . . . . . . . . . 79
3417-10 . . . . . . . . . . . . . . . . . . . . . . 109
3468 . . . . . . . . . . . . . . . . . . . . . . . . . 104
3469 . . . . . . . . . . . . . . . . . . . . . . . . . 104
3484 . . . . . . . . . . . . . . . . . . . . . . . . . 104
3485 . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3491 . . . . . . . . . . . . . . . . . . . . . 109, 160
3492 . . . . . . . . . . . . . . . . . . . . . . . . . 109
3508 . . . . . . . . . . . . . . . . . . . . . . . . . 109
Item
PRODUCT INDEX
Item
Page
500186. . . . . . . . . . . . . . . 128, 149, 155
500191. . . . . . . . . . . . . . . . . . . . . . . 181
500192. . . . . . . . . . . . . . . . . . . . . . . 181
500193. . . . . . . . . . . . . . . . . . . . . . . 181
500194. . . . . . . . . . . . . . . . . . . . . . . 181
500195. . . . . . . . . . . . . . . . . . . . . . . 181
500196. . . . . . . . . . . . . . . . . . . . . . . 181
500197. . . . . . . . . . . . . . . . . . . . . . . 181
500198. . . . . . . . . . . . . . . . . . . . . . . 181
500199. . . . . . . . . . . . . . . . . . . . . . . 181
500200. . . . . . . . . . . . . . . . . . . . . . . 181
500201. . . . . . . . . . . . . . . . . . . . . . . 181
500202. . . . . . . . . . . . . . . . . . . . . . . 181
500203. . . . . . . . . . . . . . . . . . . . . . . 181
500204. . . . . . . . . . . . . . . . . . . . . . . 181
500205. . . . . . . . . . . . . . . . . . . . . . . 181
500206. . . . . . . . . . . . . . . . . . . . . . . 181
500207. . . . . . . . . . . . . . . . . . . . . . . 181
500216. . . . . . . . . . . . . . . . . . . . . . . . 25
500216-G . . . . . . . . . . . . . . . . . . . . . . 25
500217. . . . . . . . . . . . . . . . . . . . . . . . 25
500217-G . . . . . . . . . . . . . . . . . . . . . . 25
500233. . . . . . . . . . . . . . . . . . . . . . . . 21
500234. . . . . . . . . . . . . . . . . . . . . . . . 22
500236. . . . . . . . . . . . . . . . . . . . . . . . 27
500236-G . . . . . . . . . . . . . . . . . . . . . . 27
500239-500248. . . . . . . . . . . . . . . . . 27
500240. . . . . . . . . . . . . . . . . . . . . . . . 27
500252. . . . . . . . . . . . . . . . . . . . . . . . 32
500253. . . . . . . . . . . . . . . . . . . . . . . . 32
500254. . . . . . . . . . . . . . . . . . . . . . . . 32
500255. . . . . . . . . . . . . . . . . . . . . . . . 32
500256. . . . . . . . . . . . . . . . . . . . . . . 109
500257. . . . . . . . . . . . . . . . . . . . . . . 109
500258. . . . . . . . . . . . . . . . . . . . . . . 109
500259. . . . . . . . . . . . . . . . . . . . . . . 109
500260. . . . . . . . . . . . . . . . . . . . . . . . 24
500260-G . . . . . . . . . . . . . . . . . . . . . . 24
500261. . . . . . . . . . . . . . . . . . . . . . . 149
500262. . . . . . . . . . . . . . . . . . . . . . . 149
500264. . . . . . . . . . . . . . . . . . . . . . . 149
500266. . . . . . . . . . . . . . . . . . . . . . . 149
500280. . . . . . . . . . . . . . . . . . . . . . . 102
500282. . . . . . . . . . . . . . . . . . . . . . . 168
500283. . . . . . . . . . . . . . . . . . . . . . . 168
500292. . . . . . . . . . . . . . . . . . . 125, 127
500299. . . . . . . . . . . . . . . . . . . . . . . 186
500329. . . . . . . . . . . . . . . . . . . . . . . 125
500341. . . . . . . . . . . . . . . . . . . . . . . . 21
500342. . . . . . . . . . . . . . . . . . . . . . . . 21
500343. . . . . . . . . . . . . . . . . . . . . . . . 27
500344. . . . . . . . . . . . . . . . . . . . . . . . 27
500345. . . . . . . . . . . . . . . . . . . . . . . . 27
500346. . . . . . . . . . . . . . . . . . . . . . . . 27
500348-500356. . . . . . . . . . . . . . . . . 27
500353. . . . . . . . . . . . . . . . . . . . . . . . 27
500370. . . . . . . . . . . . . . . . . . . . . . . . 31
500475. . . . . . . . . . 140, 141, 142, 143
500476. . . . . . . . . . 140, 141, 142, 143
500778. . . . . . . . . . . . . . . . . . . . . . . 186
500871. . . . . . . . . . . . . . . . . . . . . . . 144
500890. . . . . . . . . . . . . . . . . . . . . . . 106
500895. . . . . . . . . . . . . . . . . . . . . . . 106
501218. . . . . . . . . . . . . . . . . . . . . . . . 26
501218-G . . . . . . . . . . . . . . . . . . . . . . 26
501219. . . . . . . . . . . . . . . . . . . . . . . . 26
501219-G . . . . . . . . . . . . . . . . . . . . . . 26
501220. . . . . . . . . . . . . . . . . . . . . . . . 26
501220-G . . . . . . . . . . . . . . . . . . . . . . 26
501221. . . . . . . . . . . . . . . . . . . . . . . . 26
501221-G . . . . . . . . . . . . . . . . . . . . . . 26
501222. . . . . . . . . . . . . . . . . . . . . . . . 26
501222-G . . . . . . . . . . . . . . . . . . . . . . 26
501232. . . . . . . . . . . . . . . . . . . . . . . . 24
501234. . . . . . . . . . . . . . . . . . . . . . . . 24
501234-G . . . . . . . . . . . . . . . . . . . . . . 24
501241. . . . . . . . . . . . . . . . . . . . . . . . 23
501241-G . . . . . . . . . . . . . . . . . . . . . . 23
501288. . . . . . . . . . . . . . . . . . . . . . . . 23
501288-G . . . . . . . . . . . . . . . . . . . . . . 23
501291. . . . . . . . . . . . . . . . . . . . . . . . 23
501291-G . . . . . . . . . . . . . . . . . . . . . . 23
501321. . . . . . . . . . . . . . . . . . . . . . . 103
210
Item
Page
501331. . . . . . . . . . . . . . . . . . . . . . . . 31
501336. . . . . . . . . . . . . . . . . . . . . . . . 28
501352. . . . . . . . . . . . . . . . . . . . . . . 151
501353. . . . . . . . . . . . . . . . . . . . . . . 151
501355. . . . . . . . . . . . . . . . . . . . . . . 151
501369. . . . . . . . . . . . . . . . . . . . . . . 151
501370. . . . . . . . . . . . . . . . . . . . . . . 151
501371. . . . . . . . . . . . . . . . . . . . . . . 151
501372. . . . . . . . . . . . . . . . . . . . . . . 151
501373. . . . . . . . . . . . . . . . . . . . . . . 151
501375. . . . . . . . . . . . . . . . . . . . . . . 151
501376. . . . . . . . . . . . . . . . . . . . . . . 151
501377. . . . . . . . . . . . . . . . . . . . . . . 151
501378. . . . . . . . . . . . . . . . . . . . . . . 151
501379. . . . . . . . . . . . . . . . . . . . . . . 151
501381. . . . . . . . . . . . . . . . . . . . . . . 151
501601. . . . . . . . . . . . . . . . . . . . . . . 103
501607. . . . . . . . . . . . . . . . . . . . . . . 137
501608. . . . . . . . . . . . . . . . . . . . . . . . 50
501609. . . . . . . . . . . . . . . . . . . 195, 201
501610. . . . . . . . . . . . . . . . . . . . . . . 103
501622. . . . . . . . . . . . . . . 134, 136, 137
501623. . . . . . . . . . . . . . . 134, 136, 137
501624. . . . . . . . . . . . . . . . . . . . . . . 134
501635. . . . . . . . . . . . . . . . . . . . . . . 103
501636. . . . . . . . . . . . . . . . . . . . . . . 154
501637. . . . . . . . . . . . . . . . . . . . . . . 154
501641. . . . . . . . . . . . . . . . . . . . . . . . 64
501642. . . . . . . . . . . . . . . . . . . . . . . . 64
501643. . . . . . . . . . . . . . . . . . . . . . . . 64
501644. . . . . . . . . . . . . . . . . . . . . 64, 65
501651. . . . . . . . . . . . . . . . . . . . . . . 146
501652. . . . . . . . . . . . . . . . . . . . . . . 146
501653. . . . . . . . . . . . . . . . . . . . . . . 146
501656. . . . . . . . . . . . . . . . . . . . . . . . 64
501656-C . . . . . . . . . . . . . . . . . . . . . . 64
501656-F. . . . . . . . . . . . . . . . . . . . . . . 64
501657. . . . . . . . . . . . . . . . . . . . . . . . 64
501658. . . . . . . . . . . . . . . . . . . . . . . . 64
501658-C . . . . . . . . . . . . . . . . . . . . . . 64
501658-F. . . . . . . . . . . . . . . . . . . . . . . 64
501670. . . . . . . . . . . . . . . . . . . . . . . 109
501705. . . . . . . . . . . . . . . . . . . . . . . . 23
501705-G . . . . . . . . . . . . . . . . . . . . . . 23
501714. . . . . . . . . . . . . . . . . . . . . . . . 23
501714-G . . . . . . . . . . . . . . . . . . . . . . 23
501715. . . . . . . . . . . . . . . . . . . . . . . . 23
501715-G . . . . . . . . . . . . . . . . . . . . . . 23
501728. . . . . . . . . . . . . . . . . . . . . . . . 32
501729. . . . . . . . . . . . . . . . . . . . . . . . 32
501743. . . . . . . . . . . . . . . . . . . . . . . . 26
501743-G . . . . . . . . . . . . . . . . . . . . . . 26
501753. . . . . . . . . . . . . . . . . . . . . . . . 26
501753-G . . . . . . . . . . . . . . . . . . . . . . 26
501754. . . . . . . . . . . . . . . . . . . . . . . . 26
501754-G . . . . . . . . . . . . . . . . . . . . . . 26
501755. . . . . . . . . . . . . . . . . . . . . . . . 26
501755-G . . . . . . . . . . . . . . . . . . . . . . 26
501756. . . . . . . . . . . . . . . . . . . . . . . . 26
501756-G . . . . . . . . . . . . . . . . . . . . . . 26
501757. . . . . . . . . . . . . . . . . . . . . . . . 26
501757-G . . . . . . . . . . . . . . . . . . . . . . 26
501758. . . . . . . . . . . . . . . . . . . . . . . . 25
501758-G . . . . . . . . . . . . . . . . . . . . . . 25
501759. . . . . . . . . . . . . . . . . . . . . . . . 25
501759-G . . . . . . . . . . . . . . . . . . . . . . 25
501778. . . . . . . . . . . . . . . . . . . . . . . . 24
501839. . . . . . . . . . . . . . . . . . . . . . . . 24
501842. . . . . . . . . . . . . . . . . . . . . . . . 29
501850-501858. . . . . . . . . . . . . . . . . 29
501851. . . . . . . . . . . . . . . . . . . . . . . . 29
501852. . . . . . . . . . . . . . . . . . . . . . . . 29
501853. . . . . . . . . . . . . . . . . . . . . . . . 29
501854. . . . . . . . . . . . . . . . . . . . . . . . 29
501855. . . . . . . . . . . . . . . . . . . . . . . . 29
501856. . . . . . . . . . . . . . . . . . . . . . . . 29
501857. . . . . . . . . . . . . . . . . . . . . . . . 29
501858. . . . . . . . . . . . . . . . . . . . . . . . 29
501860-501864. . . . . . . . . . . . . . . . . 29
501862. . . . . . . . . . . . . . . . . . . . . . . . 29
501863. . . . . . . . . . . . . . . . . . . . . . . . 29
501864. . . . . . . . . . . . . . . . . . . . . . . . 29
501976. . . . . . . . . . . . . . . . . . . . . . . . 22
Item
Page
501976-6 . . . . . . . . . . . . . . . . . . . . . . 22
501979. . . . . . . . . . . . . . . . . . . . . . . . 22
501979-6 . . . . . . . . . . . . . . . . . . . . . . 22
501981. . . . . . . . . . . . . . . . . . . . . . . . 22
501981-6 . . . . . . . . . . . . . . . . . . . . . . 22
501985. . . . . . . . . . . . . . . . . . . . . . . . 21
501986. . . . . . . . . . . . . . . . . . . . . . . 102
502000. . . . . . . . . . . . . . . . . . . . . . . 149
502001. . . . . . . . . . . . . . . . . . . . . . . 149
502004. . . . . . . . . . . . . . . . . . . 149, 151
502005. . . . . . . . . . . . . . . . . . . 149, 151
502006. . . . . . . . . . . . . . . . . . . 149, 151
502007. . . . . . . . . . . . . . . . . . . 149, 151
502009. . . . . . . . . . . . . . . . . . . . . . . 149
502010. . . . . . . . . . . . . . . . . . . . . . . 149
502011. . . . . . . . . . . . . . . . . . . . . . . 149
502012. . . . . . . . . . . . . . . . . . . . . . . 149
502013. . . . . . . . . . . . . . . . . . . . . . . 149
502015. . . . . . . . . . . . . . . . . . . 149, 155
502016. . . . . . . . . . . . . . . . . . . . . . . 149
502017. . . . . . . . . . . . . . . . . . . . . . . 149
502018. . . . . . . . . . . . . . . . . . . . . . . 149
502019. . . . . . . . . . . . . . . . . . . . . . . 149
502040. . . . . . . . . . . . . . . . . . . . . . . 164
502041. . . . . . . . . . . . . . . . . . . . . . . 164
502053. . . . . . . . . . . . . . . . . . . . . . . . 47
502054. . . . . . . . . . . . . . . . . . . . . . . . 47
502055. . . . . . . . . . . . . . . . . . . . . . . . 47
502056. . . . . . . . . . . . . . . . . . . . . . . . 47
502060. . . . . . . . . . . . . . . . . . . . . . . . 46
502062. . . . . . . . . . . . . . . . . . . . . . . . 46
502063. . . . . . . . . . . . . . . . . . . . . . . . 46
502067. . . . . . . . . . . . . . . . . . . . . . . . 47
502068. . . . . . . . . . . . . . . . . . . . . . . . 47
502070. . . . . . . . . . . . . . . . . . . . . . . . 47
502102. . . . . . . . . . . . . . . . . . . . . . . 134
502105. . . . . . . . . . . . . . . . . . . . . . . 140
502109. . . . . . . . . . . . . . . . . . . . . . . 169
502110. . . . . . . . . . . . . . . . . . . . . . . 169
502119. . . . . . . . . . . . . . . . . . . . . . . . 65
502120. . . . . . . . . . . . . . . . . . . . . . . . 65
502122. . . . . . . . . . . . . . . . . . . . . . . . 65
502123. . . . . . . . . . . . . . . . . . . . . . . . 65
502124. . . . . . . . . . . . . . . . . . . . . . . . 65
502125. . . . . . . . . . . . . . . . . . . . . . . 169
502157. . . . . . . . . . . . . . . . . . . . . . . 103
502160. . . . . . . . . . . . . . . . . . . . . . . 149
502163. . . . . . . . . . . . . . . . . . . 149, 151
502167. . . . . . . . . . . . . . . . . . . . . . . 149
502168. . . . . . . . . . . . . . . . . . . . . . . 149
502190. . . . . . . . . . . . . . . . . . . . . . . 110
502191. . . . . . . . . . . . . . . . . . . . . . . 110
502193-4 . . . . . . . . . . . . . . . . . . . . . 111
502195. . . . . . . . . . . . . . . . . . . . . . . . 40
502196. . . . . . . . . . . . . . . . . . . . . . . . 40
502197. . . . . . . . . . . . . . . . . . . . . . . . 40
502201. . . . . . . . . . . . . . . . . . . . 47, 183
502204. . . . . . . . . . . . . . . . . . . . . . . . 46
502210. . . . . . . . . . . . . . . . . . . . . . . . 47
502213. . . . . . . . . . . . . . . . . . . . . 46, 47
502224. . . . . . . . . . . . . . . . . . . . . . . . 47
502225. . . . . . . . . . . . . . . . . . . . . . . . 47
502226. . . . . . . . . . . . . . . . . . . . . . . . 46
502227. . . . . . . . . . . . . . . . . . . . . . . . 48
502228. . . . . . . . . . . . . . . . . . . . . . . . 48
502229. . . . . . . . . . . . . . . . . . . . . . . . 48
502230. . . . . . . . . . . . . . . . . . . . . . . . 48
502231. . . . . . . . . . . . . . . . . . . . . . . . 48
502232. . . . . . . . . . . . . . . . . . . . . . . . 48
502233. . . . . . . . . . . . . . . . . . . . . . . . 48
502234. . . . . . . . . . . . . . . . . . . . . . . . 48
502235. . . . . . . . . . . . . . . . . . . . . . . . 47
502236. . . . . . . . . . . . . . . . . . . . . . . . 47
502237. . . . . . . . . . . . . . . . . . . . . 29, 47
502238. . . . . . . . . . . . . . . . . . . . . . . . 46
502241. . . . . . . . . . . . . . . . . . . . . . . . 46
502242. . . . . . . . . . . . . . . . . . . . . . . . 47
502243. . . . . . . . . . . . . . . . . . . . . . . . 47
502244. . . . . . . . . . . . . . . . . . . . . . . . 47
502245. . . . . . . . . . . . . . . . . . . . . . . . 47
502300. . . . . . . . . . . . . . . . . . . . . . . . 45
502303. . . . . . . . . . . . . . . . . . . . . . . . 45
502350. . . . . . . . . . . . . . . . . . . . . . . . 45
Item
502353. . . . . . . . . . . . . . . . . . . . . . . . 45
502600. . . . . . . . . . . . . . . . . . . . . . . . 44
502603. . . . . . . . . . . . . . . . . . . . . . . . 44
502650. . . . . . . . . . . . . . . . . . . . . . . . 44
502653. . . . . . . . . . . . . . . . . . . . . . . . 44
503022. . . . . . . . . . . . . . . . . . . . . . . 167
503023. . . . . . . . . . . . . . . . . . . . . . . 167
503036. . . . . . . . . . . . . . . . . . . . . . . 103
503041. . . . . . . . . . . . . . . . . . . . . . . 111
503042. . . . . . . . . . . . . . . . . . . . . . . 111
503049. . . . . . . . . . . . . . . . . . . . . . . 167
503050. . . . . . . . . . . . . . . . . . . . . . . 167
503051. . . . . . . . . . . . . . . . . . . . . . . 151
503067. . . . . . . . . . . . . . . . . . . . . . . . 43
503070. . . . . . . . . . . . . . . . . . . . . . . 110
503071. . . . . . . . . . . . . . . . . . . . . . . 110
503072. . . . . . . . . . . . . . . . . . . . . . . 110
503073. . . . . . . . . . . . . . . . . . . . . . . 110
503075. . . . . . . . . . . . . . . . . . . . . . . 110
503076. . . . . . . . . . . . . . . . . . . . . . . 110
503077. . . . . . . . . . . . . . . . . . . . . . . 110
503078-4 . . . . . . . . . . . . . . . . . . . . . 111
503079-4 . . . . . . . . . . . . . . . . . . . . . 111
503080-4 . . . . . . . . . . . . . . . . . . . . . 111
503081-4 . . . . . . . . . . . . . . . . . . . . . 110
503082-4 . . . . . . . . . . . . . . . . . . . . . 111
503083. . . . . . . . . . . . . . . . . . . . . . . 110
503084. . . . . . . . . . . . . . . . . . . . . . . 110
503085. . . . . . . . . . . . . . . . . . . . . . . 110
503086. . . . . . . . . . . . . . . . . . . . . . . 111
503088-10 . . . . . . . . . . . . . . . . . . . . 110
503097. . . . . . . . . . . . . . . . . . . . . . . 162
503098. . . . . . . . . . . . . . . . . . . . . . . 162
503099. . . . . . . . . . . . . . . . . . . . . . . 162
503102. . . . . . . . . . . . . . . . . . . . . . . 151
503110. . . . . . . . . . . . . . . . . . . . . . . . 65
503120. . . . . . . . . . . . . . . . . . . 167, 168
503121. . . . . . . . . . . . . . . . . . . . . . . 168
503122. . . . . . . . . . . . . . . . . . . . . . . 168
503235. . . . . . . . . . . . . . . . . . . . . . . . 21
503259. . . . . . . . . . . . . . . . . . . . . . . . 25
503260. . . . . . . . . . . . . . . . . . . . . . . . 25
503301. . . . . . . . . . . . . . . . . . . . . . . . 99
503505. . . . . . . . . . . . . . . . . . . . . . . 164
503506. . . . . . . . . . . . . . . . . . . . . . . 164
503507. . . . . . . . . . . . . . . . . . . . . . . 164
503509. . . . . . . . . . . . . . . . . . . . . . . 164
503510. . . . . . . . . . . . . . . . . . . . . . . 152
503511. . . . . . . . . . . . . . . . . . . . . . . 152
503512. . . . . . . . . . . . . . . . . . . . . . . 152
503513. . . . . . . . . . . . . . . . . . . 127, 153
503514. . . . . . . . . . . . . . . . . . . 127, 153
503524. . . . . . . . . . . . . . . . . . . . . . . . 40
503529. . . . . . . . . . . . . . . . . . . . . . . 104
503530. . . . . . . . . . . . . . . . . . . . . . . 104
503531. . . . . . . . . . . . . . . . . . . . . . . 104
503532. . . . . . . . . . . . . . . . . . . . . . . 104
503536. . . . . . . . . . . . . . . . . . . 109, 161
503537. . . . . . . . . . . . . . . . . . . . . . . 104
503567. . . . . . . . . . . . . . . . . . . . . . . . 47
503568. . . . . . . . . . . . . . . . . . . . . . . 146
503569. . . . . . . . . . . . . . . . . . . . . . . 146
503570. . . . . . . . . . . . . . . . . . . . . . . 146
503571. . . . . . . . . . . . . . . . . . . . . . . 146
503573. . . . . . . . . . . . . . . . . . . . . . . . 40
503598. . . . . . . . . . . . . . . . . . . . . 29, 47
503599. . . . . . . . . . . . . . . . . . . . . 29, 47
503622. . . . . . . . . . . . . . . . . . . . . . . . 31
503640. . . . . . . . . . . . . . . . . . . . . . . . 31
503641. . . . . . . . . . . . . . . . . . . . . . . . 31
503642. . . . . . . . . . . . . . . . . . . . . . . . 31
503645. . . . . . . . . . . . . . . . . . . . . . . . 31
503646. . . . . . . . . . . . . . . . . . . . . . . . 31
503666. . . . . . . . . . . . . . . . . . . . . . . . 25
503667. . . . . . . . . . . . . . . . . . . . . . . . 25
503668. . . . . . . . . . . . . . . . . . . . . . . . 25
503669. . . . . . . . . . . . . . . . . . . . . . . . 25
503670. . . . . . . . . . . . . . . . . . . . . . . . 25
503671. . . . . . . . . . . . . . . . . . . . . . . . 25
503763. . . . . . . . . . . . . . . . . . . . . . . 100
503843. . . . . . . . . . . . . . . . . . . . . . . . 20
503844. . . . . . . . . . . . . . . . . . . . . . . . 20
503847. . . . . . . . . . . . . . . . . . . . . . . 198
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
Page
US: Tel: 941-371-1003 • [email protected]
Item
Page
Page
600009. . . . . . . . . . . . . . . . . . . . . . . 101
600011. . . . . . . . . . . . . . . . . . . . . 53, 56
600012. . . . . . . . . . . . . . . . . . . . . 53, 56
600015. . . . . . . . . . . . . . . . . . . . . . . . 53
600016. . . . . . . . . . . . . . . . . . . . . . . . 53
800100. . . . . . . . . . . . . . . . . . . . . . . . 57
800120. . . . . . . . . . . . . . . . . . . . . . . 199
800292. . . . . . . . . . . . . . . . . . . . . . . 127
801566. . . . . . . . . . . . . . . . . . . . . . . 185
801824. . . . . . . . . . . . . . . . . . . . . . . 109
801963. . . . . . . . . . . . . . . . . . . . . . . 185
µTip. . . . . . . . . . . . . . . . . . . . . . . . . . 117
A
A300 Pulsemaster . . . . . . . . . . . . . . . . 94
A310 Accupulser . . . . . . . . . . . . . . . . . 93
A320D. . . . . . . . . . . . . . . . . . . . . . . . . 95
A320R. . . . . . . . . . . . . . . . . . . . . . . . . 95
A362 . . . . . . . . . . . . . . . . . . . . . . . 95, 98
A365D. . . . . . . . . . . . . . . . . . . . . . . . . 96
A365R. . . . . . . . . . . . . . . . . . . . . . . . . 96
A382 . . . . . . . . . . . . . . . . . . . . . . . . . . 97
A385 . . . . . . . . . . . . . . . . . . . . . . . . . . 97
A385R. . . . . . . . . . . . . . . . . . . . . . . . . 97
A385RC. . . . . . . . . . . . . . . . . . . . . . . . 97
A395 . . . . . . . . . . . . . . . . . . . . . . . . . . 98
A395D. . . . . . . . . . . . . . . . . . . . . . . . . 98
A395R. . . . . . . . . . . . . . . . . . . . . . . . . 98
A395RC. . . . . . . . . . . . . . . . . . . . . . . . 98
ABM. . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Accupulser™ Signal Generator . . . . . . . 93
Action Potential Sensor. . . . . . . . . . 7, 8, 9
Adhesives. . . . . . . . . . . . . . . . . . 100, 101
ADPT2 Footswitch . . . . . . . . . . . . . . . 177
Ag/AgCl Half-Cells . . . . . . . . . . . . . . . 119
AGT0525. . . . . . . . . . . . . . . . . . . . . . 105
AGT1010. . . . . . . . . . . . . . . . . . . . . . 105
AGT1025. . . . . . . . . . . . . . . . . . . . . . 105
AGT1510. . . . . . . . . . . . . . . . . . . . . . 105
AGT1530. . . . . . . . . . . . . . . . . . . . . . 105
AGT05100. . . . . . . . . . . . . . . . . . . . . 105
AGT10100. . . . . . . . . . . . . . . . . . . . . 105
AGTxxxx. . . . . . . . . . . . . . . . . . . . . . . 105
AGW0530 . . . . . . . . . . . . . . . . . . . . . 105
AGW1010 . . . . . . . . . . . . . . . . . . . . . 105
AGW1030 . . . . . . . . . . . . . . . . . . . . . 105
AGW1510 . . . . . . . . . . . . . . . . . . . . . 105
AGW1530 . . . . . . . . . . . . . . . . . . . . . 105
AGW2010 . . . . . . . . . . . . . . . . . . . . . 105
AGW2030 . . . . . . . . . . . . . . . . . . . . . 105
AGW4010 . . . . . . . . . . . . . . . . . . . . . 105
AGWxxxx. . . . . . . . . . . . . . . . . . . . . . 105
Air-Therm. . . . . . . . . . . . . . . . . . . . . . 160
AIRTHERMY-ATX . . . . . . . . . . . . . . . . 160
AIRTHERMZ-ATX. . . . . . . . . . . . . . . . 160
AL1000 . . . . . . . . . . . . . . . . . . . . . . . 177
AL-1000HP . . . . . . . . . . . . . . . . . . . . 177
AL2000 . . . . . . . . . . . . . . . . . . . . . . . 177
Aladdin Syringe Pumps . . . . . . . . . . . 177
allodynia . . . . . . . . . . . . . . . . . . . . . . . 36
Alumina Abrasive. . . . . . . . . . . . . . . . 128
Amplifier Selection Guide. . . . . 71, 83, 85
Analgesia. . . . . . . . . . . . . . . . . . . . . . . 37
Analgesia Meter. . . . . . . . . . . . . . . 38, 39
Anesthesia . . . . . . . . . . . . . . . . . . . . . . 34
Anesthesiometer. . . . . . . . . . . . . . . . . . 36
Angled Electrode Holder. . . . . . . . . . . 140
Animal Temperature Controller. . . . . . . 40
Anti-Oscillation Module . . . . . . . . . . . . 84
APEH1. . . . . . . . . . . . . . . . . . . . . . . . 120
APEH1xx . . . . . . . . . . . . . . . . . . . . . . 121
APEH2. . . . . . . . . . . . . . . . . . . . . . . . 120
APEH2xx . . . . . . . . . . . . . . . . . . . . . . 121
Articulated Arm . . . . . . . . . . . . . 149, 151
ATC2000 . . . . . . . . . . . . . . . . . . . . . . . 40
Audible Baseline Monitor. . . . . . . . . . . 81
autoclavable pipetters. . . . . . . . . . . . . 178
Autoclave. . . . . . . . . . . . . . . . . . . . . . . 32
Automated TEER Measuring System. . . 16
AUW201 . . . . . . . . . . . . . . . . . . . . . . 105
AUWxxxx. . . . . . . . . . . . . . . . . . . . . . 105
Item
Page
B
Ball Bearing Boom Stand. . . . . . 149, 151
Ball-joint holder attachment. . . . . . . . 141
Ball Joint, Magnetic . . . . . . . . . . . . . . 144
Barb-to-Tubing Coupler . . . . . . . . . . . 106
Base. . . . . . . . . . . . . . . . . . . . . . . . . . 110
Base, Magnetic. . . . . . . . . . . . . . . . . . 145
base plate. . . . . . . . . . . . . . . . . . . . . . 146
BAT-10R/LOP. . . . . . . . . . . . . . . . . . . . 50
BAT-10R/LOP220. . . . . . . . . . . . . . . . . 50
BAT-12R. . . . . . . . . . . . . . . . . . . . . . . . 50
BAT-12R-220. . . . . . . . . . . . . . . . . . . . 50
Bath, Circulating. . . . . . . . . . . . . . . . . . 20
Battery Charger . . . . . . . . . . . . . . . 95, 97
Battery, NiMH. . . . . . . . . . . . . . . . . . . . 79
Beakers . . . . . . . . . . . . . . . . . . . . . . . 112
Beam splitter . . . . . . . . . . . . . . . . . . . 154
BEECAL . . . . . . . . . . . . . . . . . . . . . . . . 67
Beetrode. . . . . . . . . . . . . . . . . . . . . . . . 67
Beveler, Micropipette . . . . . . . . . . . . . 128
Biaxial Test System. . . . . . . . . . . . . . . . 10
BIF22. . . . . . . . . . . . . . . . . . . . . . . . . 208
BIF41. . . . . . . . . . . . . . . . . . . . . . . . . 208
BIF44. . . . . . . . . . . . . . . . . . . . . . . . . 208
BIF62. . . . . . . . . . . . . . . . . . . . . . . . . 208
BIF66. . . . . . . . . . . . . . . . . . . . . . . . . 208
Bifurcated Fiber . . . . . . . . . . . . . . . . . 208
Bifurcated Light Guide . . . . . . . . . . . . 155
Bio-amplifier. . . . . . . . . . . . . . . . . . . . . 78
BioClave Mini. . . . . . . . . . . . . . . . . . . . 32
Bio Photometric Detection System . . . 192
BioTester. . . . . . . . . . . . . . . . . . . . . . . . 10
Bipolar Electrodes. . . . . . . . . . . . . . . . . 88
bipolar stimulation. . . . . . . . . . . . . . . . 88
Blood Pressure. . . . . . . . . . . . . . . . . . . 42
Blood Pressure Monitor . . . . . . . . . . . . 43
Blood Pressure System. . . . . . . . . . . . . 42
BLOOD PRESSURE TRANSDUCER. . . . 43
BLPR2 . . . . . . . . . . . . . . . . . . . . . . . . . 43
Boom Stand. . . . . . . . . . . . . . . . 149, 151
Borosilicate. . . . . . . . 114, 116, 117, 131
BP1 . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
BPCABLE2 . . . . . . . . . . . . . . . . . . . . . . 43
Brain Matrices . . . . . . . . . . . . . . . . . . . 20
Bridge Amplifier. . . . . . . . . . . . . . . . . . 83
BT-1. . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Buffer Solutions . . . . . . . . . . . . . . . . . . 68
Buffer Temperature Sensor. . . . . . . 7, 8, 9
Bulldog Clamps . . . . . . . . . . . . . . . . . . 27
C
C3005 . . . . . . . . . . . . . . . . . . . . . . . . 105
Cables and Connectors. . . . . . . . . . . . 108
CAL900A . . . . . . . . . . . . . . . . . . . . . . . 90
CALBUF-1. . . . . . . . . . . . . . . . . . . . . . . 68
CALBUF-2. . . . . . . . . . . . . . . . . . . . . . . 68
Calcium Calibration Solutions. . . . . . . . 68
Calcium Electrode. . . . . . . . . . . . . . . . . 69
CALICELL-12. . . . . . . . . . . . . . . . . . . . . 14
CALICELL-24. . . . . . . . . . . . . . . . . . . . . 14
Caliper, Digital. . . . . . . . . . . . . . . . . . 103
Cannula, Micro. . . . . . . . . . . . . . . . . . 117
Cannulas . . . . . . . . . . . . . . . . . . . . . . . 49
Carbon Epoxy. . . . . . . . . . . . . . . . . . . 101
Carbon fiber microelectrodes . . . . . . . . 61
Carbon Wire. . . . . . . . . . . . . . . . . . . . 105
cartridge electrodes . . . . . . . . . . . . . . . 17
Catheters . . . . . . . . . . . . . . . . . . . . . . . 52
cautery electrode . . . . . . . . . . . . . . 33, 52
CBL100 . . . . . . . . . . . . . . . . . . . . . . . 109
CBL102 . . . . . . . . . . . . . . . . . . . 109, 187
CC-3-UV. . . . . . . . . . . . . . . . . . . . . . . 206
Cell culture cups. . . . . . . . . . . . . . . . . . 14
Cell Tester System . . . . . . . . . . . . . . . . . 3
CF10-100. . . . . . . . . . . . . . . . . . . . . . . 61
CF10-250. . . . . . . . . . . . . . . . . . . . . . . 61
CF10-500. . . . . . . . . . . . . . . . . . . . . . . 61
CF30-50. . . . . . . . . . . . . . . . . . . . . . . . 61
CF30-100. . . . . . . . . . . . . . . . . . . . . . . 61
CF30-500. . . . . . . . . . . . . . . . . . . . . . . 61
CF30-1000 . . . . . . . . . . . . . . . . . . . . . 61
CFN10-50 . . . . . . . . . . . . . . . . . . . . . . 61
CFN10-100 . . . . . . . . . . . . . . . . . . . . . 61
Item
CFN10-250 . . . . . . . . . . . . . . . . . . . . . 61
CFN30-50 . . . . . . . . . . . . . . . . . . . . . . 61
CFN30-100 . . . . . . . . . . . . . . . . . . . . . 61
CFN30-250 . . . . . . . . . . . . . . . . . . . . . 61
CFN30-500 . . . . . . . . . . . . . . . . . . . . . 61
CFN30-1000 . . . . . . . . . . . . . . . . . . . . 61
CG-0001 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0002 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0003 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0004 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0005 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0006 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0007 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0008 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0009 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0010 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0011 . . . . . . . . . . . . . . . . . . 112, 113
CG-0015 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0016 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0017 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0018 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0019 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0020 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0021 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0100 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0101 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0102 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0103 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0104 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0105 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0106 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0130 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0131 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0132 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0133 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0134 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0135 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0136 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0137 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0139 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0140 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0141 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0142 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0143 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0144 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0145 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0146 . . . . . . . . . . . . . . . . . . . . . . 113
CG-0150 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0151 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0152 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0153 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0154 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0155 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0160 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0161 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0162 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0163 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0164 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0165 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0166 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0167 . . . . . . . . . . . . . . . . . . . . . . 112
CG-0168 . . . . . . . . . . . . . . . . . . 112, 113
Chamber, Measurement . . . . . . . . . . . . 57
CHM1. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM2. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM3. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM4. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM5. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM5M12. . . . . . . . . . . . . . . . . . . . . . 19
CHM6. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM7. . . . . . . . . . . . . . . . . . . . . . . . . . 19
CHM8. . . . . . . . . . . . . . . . . . . . . . . . . . 19
Circulation Reservoirs. . . . . . . . . . . . . . 19
clamp. . . . . . . . . . . . . . . . . . . . . . . . . 141
Clamps. . . . . . . . . . . . . . . . . . . . . . . . 110
Clear hose . . . . . . . . . . . . . . . . . . . . . 160
Clip Applier . . . . . . . . . . . . . . . . . . . . . 27
Clips and Clamps. . . . . . . . . . . . . . . . . 27
CMF20GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF22GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF23GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF26GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF28GxxL. . . . . . . . . . . . . . . . . . . . 118
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
Page
US: Tel: 941-371-1003 • [email protected]
211
PRODUCT INDEX
503848. . . . . . . . . . . . . . . . . . . . . . . 198
504037. . . . . . . . . . . . . . . . . . . . . . . . 31
504038. . . . . . . . . . . . . . . . . . . . . . . . 31
504039. . . . . . . . . . . . . . . . . . . . . . . . 31
504040. . . . . . . . . . . . . . . . . . . . . . . . 31
504045. . . . . . . . . . . . . . . . . . . . . 33, 52
504046. . . . . . . . . . . . . . . . . . . . . 33, 52
504047. . . . . . . . . . . . . . . . . . . . . 33, 52
504048. . . . . . . . . . . . . . . . . . . . . 33, 52
504049. . . . . . . . . . . . . . . . . . . . . 33, 52
504052. . . . . . . . . . . . . . . . . . . . . . . . 31
504053. . . . . . . . . . . . . . . . . . . . . . . . 31
504054. . . . . . . . . . . . . . . . . . . . . . . . 31
504059. . . . . . . . . . . . . . . . . . . . . . . . 31
504060. . . . . . . . . . . . . . . . . . . . . . . . 31
504106. . . . . . . . . . . . . . . . . . . . . . . . 52
504107. . . . . . . . . . . . . . . . . . . . . . . . 52
504108. . . . . . . . . . . . . . . . . . . . . . . . 52
504109. . . . . . . . . . . . . . . . . . . . . . . . 52
504110. . . . . . . . . . . . . . . . . . . . . . . . 52
504111. . . . . . . . . . . . . . . . . . . . . . . . 52
504112. . . . . . . . . . . . . . . . . . . . . . . . 52
504113. . . . . . . . . . . . . . . . . . . . . . . . 52
504114. . . . . . . . . . . . . . . . . . . . . . . . 52
504115. . . . . . . . . . . . . . . . . . . . . . . . 52
504116. . . . . . . . . . . . . . . . . . . . . . . . 52
504117. . . . . . . . . . . . . . . . . . . . . . . . 52
504118. . . . . . . . . . . . . . . . . . . . . . . . 52
504119. . . . . . . . . . . . . . . . . . . . . . . . 52
504120. . . . . . . . . . . . . . . . . . . . . . . . 52
504121. . . . . . . . . . . . . . . . . . . . . . . . 52
504122. . . . . . . . . . . . . . . . . . . . . . . . 20
504123. . . . . . . . . . . . . . . . . . . 149, 151
504128. . . . . . . . . . . . . . . . . . . . . . . 151
504129. . . . . . . . . . . . . . . . . . . . . . . 151
504134. . . . . . . . . . . . . . . . . . . . . . . 155
504136. . . . . . . . . . . . . . . . . . . . . . . 162
504138. . . . . . . . . . . . . . . . . . . . . . . 162
504141. . . . . . . . . . . . . . . . . . . . . . . . 20
504142. . . . . . . . . . . . . . . . . . . . . . . . 20
504143. . . . . . . . . . . . . . . . . . . . . . . . 52
504144. . . . . . . . . . . . . . . . . . . . . . . . 52
504145. . . . . . . . . . . . . . . . . . . . . . . . 52
504146. . . . . . . . . . . . . . . . . . . . . . . . 52
504147. . . . . . . . . . . . . . . . . . . . . . . . 52
504148. . . . . . . . . . . . . . . . . . . . . . . . 52
504149. . . . . . . . . . . . . . . . . . . . . . . . 52
504150. . . . . . . . . . . . . . . . . . . . . . . . 52
504151. . . . . . . . . . . . . . . . . . . . . . . . 52
504187. . . . . . . . . . . . . . . . . . . . . . . . 32
504188. . . . . . . . . . . . . . . . . . . . . . . . 32
504221. . . . . . . . . . . . . . . . . . . . . . . 153
504278. . . . . . . . . . . . . . . . . . . . . . . . 49
504279. . . . . . . . . . . . . . . . . . . . . . . . 49
504280. . . . . . . . . . . . . . . . . . . . . . . . 49
504281. . . . . . . . . . . . . . . . . . . . . . . . 49
504282. . . . . . . . . . . . . . . . . . . . . . . . 49
504283. . . . . . . . . . . . . . . . . . . . . . . . 49
504284. . . . . . . . . . . . . . . . . . . . . . . 154
504285. . . . . . . . . . . . . . . . . . . . . . . 154
504286. . . . . . . . . . . . . . . . . . . . . . . 154
504287. . . . . . . . . . . . . . . . . . . . . . . 154
504334. . . . . . . . . . . . . . . . . . . . . . . 179
504335. . . . . . . . . . . . . . . . . . . . . . . 179
504336. . . . . . . . . . . . . . . . . . . . . . . 179
504337. . . . . . . . . . . . . . . . . . . . . . . 179
504338. . . . . . . . . . . . . . . . . . . . . . . 179
504339. . . . . . . . . . . . . . . . . . . . . . . 179
504416. . . . . . . . . . . . . . . . . . . . . . . 153
504417. . . . . . . . . . . . . . . . . . . . . . . 153
504443. . . . . . . . . . . . . . . . . . . . . . . 153
504444. . . . . . . . . . . . . . . . . . . . . . . 153
504445. . . . . . . . . . . . . . . . . . . . . . . 153
504570. . . . . . . . . . . . . . . . . . . . . . . 161
504574. . . . . . . . . . . . . . . . . . . . . . . 161
504576. . . . . . . . . . . . . . . . . . . 172, 173
504577. . . . . . . . . . . . . . . . . . . 172, 173
504578. . . . . . . . . . 170, 171, 172, 173
504588. . . . . . . . . . . . . . . . . . . . . . . . 181
504589. . . . . . . . . . . . . . . . . . . . . . . . 181
504590. . . . . . . . . . . . . . . . . . . . . . . . 181
504591. . . . . . . . . . . . . . . . . . . . . . . . 180
504592. . . . . . . . . . . . . . . . . . . . . . . . 179
Item
PRODUCT INDEX
Item
Page
CMF31GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF34GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF35GxxL. . . . . . . . . . . . . . . . . . . . 118
CMF90UxxL. . . . . . . . . . . . . . . . . . . . 118
C-Mount Eyepiece Adapters . . . . . . . . 162
CO2-200 . . . . . . . . . . . . . . . . . . . . . . 159
CO2-300 . . . . . . . . . . . . . . . . . . . . . . 159
CO2 Analyzer. . . . . . . . . . . . . . . . . . . . 41
CO2 Controller. . . . . . . . . . . . . . . . . . 159
coaxial cables, micro . . . . . . . . . . . . . 105
COLCAM-HD. . . . . . . . . . . . . . . . . . . . 162
COLLIMATOR . . . . . . . . . . . . . . . . . . . 206
Color Video Camera . . . . . . . . . . . . . . 162
Combination pH electrode . . . . . . . . . . 67
Compact Langendorff. . . . . . . . . . . . . . . 7
Compact Magnetic Stand . . . . . . . . . . 146
Compression. . . . . . . . . . . . . . . . . . . . . 11
Concentric Bipolar Electrodes. . . . . . . . 88
Copper Alloy Wire . . . . . . . . . . . . . . . 105
Cover Slips. . . . . . . . . . . . . . . . . . . . . 164
CP-5. . . . . . . . . . . . . . . . . . . . . . . . . . 179
CP-25. . . . . . . . . . . . . . . . . . . . . . . . . 179
CP-100. . . . . . . . . . . . . . . . . . . . . . . . 179
CP-250. . . . . . . . . . . . . . . . . . . . . . . . 179
CPVC4050G. . . . . . . . . . . . . . . . . . . . 105
CPVC4050R. . . . . . . . . . . . . . . . . . . . 105
CPVC4050W . . . . . . . . . . . . . . . . . . . 105
CPVCxxxxx. . . . . . . . . . . . . . . . . . . . . 105
CS-BIOTESTER. . . . . . . . . . . . . . . . . . . 10
CS-MECHANOCULTURE. . . . . . . . . . . . 11
CS-MICROSQUISHER. . . . . . . . . . . . . . 11
CS-USTRETCH . . . . . . . . . . . . . . . . . . . 12
CU7T44G. . . . . . . . . . . . . . . . . . . . . . 105
Current Clamp . . . . . . . . . . . . . . . . . . . 17
Current Electrode . . . . . . . . . . . . . . . . . 17
Current Generator. . . . . . . . . . . . . . . . . 81
Current Sources . . . . . . . . . . . . . . . . . . 96
CUV1022-10. . . . . . . . . . . . . . . . . . . 205
CUV2011-1. . . . . . . . . . . . . . . . . . . . 205
CUV2012-1. . . . . . . . . . . . . . . . . . . . 205
CUV2023-1. . . . . . . . . . . . . . . . . . . . 205
CUV2025-1. . . . . . . . . . . . . . . . . . . . 205
CUV2028-1. . . . . . . . . . . . . . . . . . . . 205
CUV2031-1. . . . . . . . . . . . . . . . . . . . 205
CUV2032-1. . . . . . . . . . . . . . . . . . . . 205
CUV2033-1. . . . . . . . . . . . . . . . . . . . 205
CUV2034-1. . . . . . . . . . . . . . . . . . . . 205
CUV2051-1. . . . . . . . . . . . . . . . . . . . 205
CUV2052-1. . . . . . . . . . . . . . . . . . . . 205
CUV2061-1. . . . . . . . . . . . . . . . . . . . 205
CUV2062-1. . . . . . . . . . . . . . . . . . . . 205
CUV2063-1. . . . . . . . . . . . . . . . . . . . 205
CUV2065-1. . . . . . . . . . . . . . . . . . . . 205
CUV2066-1. . . . . . . . . . . . . . . . . . . . 205
CUV2071-1. . . . . . . . . . . . . . . . . . . . 205
CUV2101-1. . . . . . . . . . . . . . . . . . . . 205
CUV2102-1. . . . . . . . . . . . . . . . . . . . 205
CUV2105-1. . . . . . . . . . . . . . . . . . . . 205
CUV2106-1. . . . . . . . . . . . . . . . . . . . 205
CUV2107-1. . . . . . . . . . . . . . . . . . . . 205
CUV2108-1. . . . . . . . . . . . . . . . . . . . 205
CUV2614-1. . . . . . . . . . . . . . . . . . . . 205
CUV2674-1. . . . . . . . . . . . . . . . . . . . 205
Cuvettes. . . . . . . . . . . . . . . . . . . . . . . 205
CW-MICROCAPSTAR . . . . . . . . . . . . . . 41
CW-MRI-1 . . . . . . . . . . . . . . . . . . . . . . 41
CW-SAR1000. . . . . . . . . . . . . . . . . . . . 41
Cyanoacrylate. . . . . . . . . . . . . . . . . . . . 49
Cylinder, Graduated . . . . . . . . . . . . . . 112
D
D4H. . . . . . . . . . . . . . . . . . . . . . . . . . 198
Dam50. . . . . . . . . . . . . . . . . . . . . . . . . 78
Dam80. . . . . . . . . . . . . . . . . . . . . . . . . 79
DAM80P. . . . . . . . . . . . . . . . . . . . . . . . 79
DAM Series Bioamplifiers. . . . . . . . . . . 78
DC3001L. . . . . . . . . . . . . . . . . . . . . . 137
DC3001R. . . . . . . . . . . . . . . . . . . . . . 137
DC3314L. . . . . . . . . . . . . . . . . . . . . . 137
DC3314R. . . . . . . . . . . . . . . . . . . . . . 137
DCAP. . . . . . . . . . . . . . . . . . . . . . . . . . 29
DCAP-L. . . . . . . . . . . . . . . . . . . . . . . . . 29
DCAP-M. . . . . . . . . . . . . . . . . . . . . . . . 29
212
Item
Page
DC heater. . . . . . . . . . . . . . . . . . . . . . . 40
Deuterium halogen light. . . . . . . . . . . 198
Differential Electrometer. . . . . . . . . . . . 72
Digital Caliper . . . . . . . . . . . . . . . . . . 103
Digital Linear Stimulus Isolator. . . . . . 99
Digital Micrometer. . . . . . . . . . . . . . . 103
Digital Micrometer Head . . . . . . . . . . 134
Digital Microscope Camera. . . . . . . . . 161
Digital Stimulator. . . . . . . . . . . . . . . . . 99
DIP-NIRx . . . . . . . . . . . . . . . . . . . . . . 203
Dipping probe . . . . . . . . . . . . . . . . . . 203
DIP-UV-MINI-x. . . . . . . . . . . . . . . . . . 203
DIP-UV-SRx. . . . . . . . . . . . . . . . . . . . 203
Dissecting Kit. . . . . . . . . . . . . . . . . . . . 28
Dissecting Pad Kit . . . . . . . . . . . . . . . 102
Dissecting Scissors. . . . . . . . . . . . . . . . 25
Dissolved oxygen meter. . . . . . . . . . . . 66
DLS100 . . . . . . . . . . . . . . . . . . . . . . . . 99
DMF1000 . . . . . . . . . . . . . . . . . 124, 126
DMF1000-1. . . . . . . . . . . . . . . . . . . . 127
DMF1000-2. . . . . . . . . . . . . . . . . . . . 127
DMF1000-H5. . . . . . . . . . . . . . . . . . . 127
DMF1000-M1 . . . . . . . . . . . . . . . . . . 127
DMF1000-M2 . . . . . . . . . . . . . . . . . . 127
DMP. . . . . . . . . . . . . . . . . . . . . . . . . . 187
DRIREF-2. . . . . . . . . . . . . . . . . . . . . . . 68
DRIREF-2SH. . . . . . . . . . . . . . . . . . . . . 68
DRIREF-5. . . . . . . . . . . . . . . . . . . . . . . 68
DRIREF-5SH. . . . . . . . . . . . . . . . . . . . . 68
DRIREF-450. . . . . . . . . . . . . . . . . . . . . 68
DRIREF-L. . . . . . . . . . . . . . . . . . . . . . . 68
Dri-Ref™. . . . . . . . . . . . . . . . . . . . . . . . 68
Driven guard shield. . . . . . . . . . . . 73, 75
DRL . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Dry Sterilizer . . . . . . . . . . . . . . . . . . . . 32
DS8000. . . . . . . . . . . . . . . . . . . . . . . . 99
Dual Microprobe System . . . . . . . . . . . 74
Dual Tool-Holder Micromanipulator. . 143
Dummy Load Resistor Kit. . . . . . . . . . . 96
Duo 773. . . . . . . . . . . . . . . . . . . . . . . . 74
E
E210 . . . . . . . . . . . . . . . . . . . . . . . . . 116
E212 . . . . . . . . . . . . . . . . . . . . . . . . . 116
E215 . . . . . . . . . . . . . . . . . . . . . . . . . 116
E220 . . . . . . . . . . . . . . . . . . . . . . . . . 116
Ear Bars, Stereotaxic. . . . . . . . . . . . . . . 47
Ear Punch. . . . . . . . . . . . . . . . . . . . . . . 28
Economy Manual Micromanipulator. . 140
Economy Tweezers. . . . . . . . . . . . . . . . 22
EHB1 . . . . . . . . . . . . . . . . . . . . . 120, 121
EHBF . . . . . . . . . . . . . . . . . . . . . 120, 121
Eight-Channel Pipetters . . . . . . . . . . . 178
EK1 . . . . . . . . . . . . . . . . . . . . . . . . 17, 19
EKC . . . . . . . . . . . . . . . . . . . . . . . . 17, 19
EKV . . . . . . . . . . . . . . . . . . . . . . . . 17, 19
Electro 705. . . . . . . . . . . . . . . . . . . . . . 73
electrochemical measurements. . . . . . . 72
Electrolyte, ISO2. . . . . . . . . . . . . . . . . . 66
Electrolyte, ISO-NO. . . . . . . . . . . . . . . . 54
electromagnetic radiation. . . . . . . . . . . 40
Electrometer. . . . . . . . . . . . . . . . . . 72, 73
electrophysiological . . . . . . . . . . . . . . . 40
Elgiloy Microelectrodes. . . . . . . . . . . . . 88
ELS-370. . . . . . . . . . . . . . . . . . . . . . . 199
ELS-xxx . . . . . . . . . . . . . . . . . . . . . . . 199
ENDOHM-6. . . . . . . . . . . . . . . . . . . . . 15
ENDOHM-12. . . . . . . . . . . . . . . . . . . . 15
ENDOHM-24SNAP. . . . . . . . . . . . . . . . 15
Endohm™. . . . . . . . . . . . . . . . . . . . . . . 15
EP1 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
EP2 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
EP4 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
EP05 . . . . . . . . . . . . . . . . . . . . . . . . . 119
EP08 . . . . . . . . . . . . . . . . . . . . . . . . . 119
EP12 . . . . . . . . . . . . . . . . . . . . . . . . . 119
Epithelial Voltohmmeter. . . . . . . . . . . . 13
Epoxy. . . . . . . . . . . . . . . . . . . . . 100, 101
Erlenmeyer Flasks . . . . . . . . . . . . . . . 113
Euthanex Lids. . . . . . . . . . . . . . . . . . . . 35
EVC3 . . . . . . . . . . . . . . . . . . . . . . . . . . 17
EVC4000 . . . . . . . . . . . . . . . . . . . . . . . 17
EVC4000-1 . . . . . . . . . . . . . . . . . . . . . 17
Item
Page
EVC4000-2 . . . . . . . . . . . . . . . . . . . . . 17
EVC4000-3 . . . . . . . . . . . . . . . . . . . . . 17
EVC4000-4 . . . . . . . . . . . . . . . . . . . . . 17
EVOM2. . . . . . . . . . . . . . . . . . . . . . . . . 13
EXT-6. . . . . . . . . . . . . . . . . . . . . . . . . . 50
Extracellular Bio-Amplifier . . . . . . . . . . 84
Extracellular recording . . . . . . . . . . . . . 78
Eyepiece Adapter, SLR . . . . . . . . . . . . 162
Eyepieces . . . . . . . . . . . . . . . . . . 149, 151
EZ-103A. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-104A. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-107A. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-109. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-177. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-178. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-179. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-211. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-212. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-320. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-330. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-830. . . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-1130. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-1785. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-7000. . . . . . . . . . . . . . . . . . . . . . . . 34
EZ-20027. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20027G . . . . . . . . . . . . . . . . . . . . . 35
EZ-20028. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20028G . . . . . . . . . . . . . . . . . . . . . 35
EZ-20029. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20030. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20030G . . . . . . . . . . . . . . . . . . . . . 35
EZ-20032. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20032G . . . . . . . . . . . . . . . . . . . . . 35
EZ-20034. . . . . . . . . . . . . . . . . . . . . . . 35
EZ-20034G . . . . . . . . . . . . . . . . . . . . . 35
EZ Anesthesia. . . . . . . . . . . . . . . . . . . . 34
EZ-B800. . . . . . . . . . . . . . . . . . . . . . . . 35
EZ-E25000. . . . . . . . . . . . . . . . . . . . . . 35
EZ-E27000. . . . . . . . . . . . . . . . . . . . . . 35
EZ-E28000. . . . . . . . . . . . . . . . . . . . . . 35
EZ-FF900. . . . . . . . . . . . . . . . . . . . . . . 34
F
FD35-100 . . . . . . . . . . . . . . . . . . . . . 163
FD35B-100 . . . . . . . . . . . . . . . . . . . . 164
FD35COL-100 . . . . . . . . . . . . . . . . . . 164
FD35PDL-100 . . . . . . . . . . . . . . . . . . 163
FD223A. . . . . . . . . . . . . . . . . . . . . . . . 72
FD223AP. . . . . . . . . . . . . . . . . . . . . . . 72
FD3510-100 . . . . . . . . . . . . . . . . . . . 163
FD5040-100 . . . . . . . . . . . . . . . . . . . 163
fiber optic cables . . . . . . . . . . . . . . . . 208
Fiber Optic Cables . . . . . . . . . . . . . . . 208
Fiber Optic Collimator. . . . . . . . . . . . . 206
Fiber Optic Filter Holder. . . . . . . . . . . 207
Fiber Optic Illuminator. . . . . . . . . . . . 155
Filling Solution. . . . . . . . . . . . . . . . . . . 53
Filter Holder. . . . . . . . . . . . . . . . . . . . 207
Filter Holder, Inline. . . . . . . . . . . . . . . 199
Fire-Polished. . . . . . . . . . . . . . . . 114, 131
Five-barrel glass. . . . . . . . . . . . . . . . . 116
Flexible NO Sensor. . . . . . . . . . . . . . . . 55
FLEXREF. . . . . . . . . . . . . . . . . . . . . . . . 68
flow analysis . . . . . . . . . . . . . . . . . . . 193
Flow Cell, Low Volume. . . . . . . . . . . . 202
Flow Cells. . . . . . . . . . . . . . . . . . . . . . 201
FLOX-PATCH. . . . . . . . . . . . . . . . . . . . . 62
FLOX-PROBE. . . . . . . . . . . . . . . . . . . . . 62
Fluorescence-based optical sensor. . . . 62
Fluorescent lamp . . . . . . . . . . . . . . . . 149
FluoroDish . . . . . . . . . . . . . . . . . . . . . 163
FO-50-SMA . . . . . . . . . . . . . . . . . . . . 208
FO-50-SMA1M. . . . . . . . . . . . . . . . . . 208
FO-100-SMA . . . . . . . . . . . . . . . . . . . 208
FO-100-SMA1M. . . . . . . . . . . . . . . . . 208
FO-200AS-SMA . . . . . . . . . . . . . . . . . 208
FO-200-SMA . . . . . . . . . . . . . . . . . . . 208
FO-200-SMA1M. . . . . . . . . . . . . . . . . 208
FO-400AS-SMA . . . . . . . . . . . . . . . . . 208
FO-400-SMA . . . . . . . . . . . . . . . . . . . 208
FO-400-SMA1M. . . . . . . . . . . . . . . . . 208
FO-400SMA/ST . . . . . . . . . . . . . . . . . 208
FO-600AS-SMA . . . . . . . . . . . . . . . . . 208
Item
Page
FO-600-SMA . . . . . . . . . . . . . . . . . . . 208
FO-600-SMA1M. . . . . . . . . . . . . 201, 208
FO-1000-SMA. . . . . . . . . . . . . . . . . . 208
FO-1000-SMA1M . . . . . . . . . . . . . . . 208
FO-6000 . . . . . . . . . . . . . . . . . . . . . . 199
FO-6000-FILT. . . . . . . . . . . . . . . . . . . 199
FOIMPH. . . . . . . . . . . . . . . . . . . 120, 121
FOIMPH-LF. . . . . . . . . . . . . . . . . 120, 121
FOP1-SMA. . . . . . . . . . . . . . . . . . . . . 208
FOP1-SMA/ST . . . . . . . . . . . . . . . . . . 208
FOP1-ST. . . . . . . . . . . . . . . . . . . . . . . 208
Forceps. . . . . . . . . . . . . . . . . . . . . . . . . 23
force transducers . . . . . . . . . . . . . . . . . 87
Force Transducers. . . . . . . . . . . . . . . . . 86
FORT10g . . . . . . . . . . . . . . . . . . . . . . . 87
FORT25 . . . . . . . . . . . . . . . . . . . . . . . . 87
FORT100. . . . . . . . . . . . . . . . . . . . . . . 87
FORT250. . . . . . . . . . . . . . . . . . . . . . . 87
FORT1000. . . . . . . . . . . . . . . . . . . . . . 87
FORT5000. . . . . . . . . . . . . . . . . . . . . . 87
FORT transducers. . . . . . . . . . . . . . . . . 87
Frame Clamp . . . . . . . . . . . . . . . . . . . 111
FrameWorks. . . . . . . . . . . . . . . . . . . . 110
Free Radical Analyzer. . . . . . . . . . . . . . 58
G
Gaskets for PicoNozzle. . . . . . . . . . . . 184
Glass Cuvettes . . . . . . . . . . . . . . . . . . 204
Glass Handling Forceps . . . . . . . . . . . 115
glass micropipettes . . . . . . . . . . . . . . 117
glass rod . . . . . . . . . . . . . . . . . . . . . . 116
glassware. . . . . . . . . . . . . . . . . . . . . . 112
Glue Gun . . . . . . . . . . . . . . . . . . 101, 118
GN-NET7. . . . . . . . . . . . . . . . . . . . . . 177
GN-NET25. . . . . . . . . . . . . . . . . . . . . 177
GN-PC7 . . . . . . . . . . . . . . . . . . . . . . . 177
GN-PC25. . . . . . . . . . . . . . . . . . . . . . 177
GN-TTL . . . . . . . . . . . . . . . . . . . . . . . 177
GO1-100. . . . . . . . . . . . . . . . . . . . . . 121
GO2-100. . . . . . . . . . . . . . . . . . . . . . 121
GO3-100. . . . . . . . . . . . . . . . . . . . . . 121
GO4-100. . . . . . . . . . . . . . . . . . . . . . 121
Gold-plated pins, sockets. . . . . . . . . . . 88
Gold Wire. . . . . . . . . . . . . . . . . . . . . . 105
GR100-4 . . . . . . . . . . . . . . . . . . . . . . 116
GR100-6 . . . . . . . . . . . . . . . . . . . . . . 116
Grip Strength Meter. . . . . . . . . . . . . . . 37
GSNO. . . . . . . . . . . . . . . . . . . . . . . . . . 57
GSNO-50. . . . . . . . . . . . . . . . . . . . . . . 57
GSNO-100. . . . . . . . . . . . . . . . . . . . . . 57
g-SPIN. . . . . . . . . . . . . . . . . . . . . . . . 104
G-SPIN6. . . . . . . . . . . . . . . . . . . . . . . 104
G-SPIN6-220. . . . . . . . . . . . . . . . . . . 104
Guide Cannula. . . . . . . . . . . . . . . . . . . 49
Guillotine. . . . . . . . . . . . . . . . . . . . . . . 29
H
Half-Cells . . . . . . . . . . . . . . . . . . . . . . 120
heart, isolated, perfused. . . . . . . . . . . . . 7
Heater Controller . . . . . . . . . . . . . . . . 160
Heating Filament . . . . . . . . . . . . 125, 127
Heating Plate . . . . . . . . . . . . . . . . . . . . 40
high throughput screening. . . . . . . . . . 16
Horizontal Tissue Bath. . . . . . . . . . . . . . 5
Hot Plate Analgesia Meter . . . . . . . . . . 39
hot spots . . . . . . . . . . . . . . . . . . . . . . . 40
HS6 . . . . . . . . . . . . . . . . . . . . . . . . . . 142
HS6-3. . . . . . . . . . . . . . . . . . . . . . . . . 137
HS6-3M. . . . . . . . . . . . . . . . . . . . . . . 137
HT-1. . . . . . . . . . . . . . . . . . . . . . . . . . . 51
HT-2. . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Hydrogen Electrode . . . . . . . . . . . . . . . 69
Hydrogen Peroxide Sensors . . . . . . 53, 56
Hydrogen Sulfide Sensor . . . . . . . . . . . 53
Hydrostatic pressure. . . . . . . . . . . . . . 187
I
IE010. . . . . . . . . . . . . . . . . . . . . . . . . .
IE190. . . . . . . . . . . . . . . . . . . . . . . . . .
IE200. . . . . . . . . . . . . . . . . . . . . . . . . .
IGS100. . . . . . . . . . . . . . . . . . . . . . . . .
II-39. . . . . . . . . . . . . . . . . . . . . . . . . . .
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
69
69
69
55
39
Item
Page
Page
ISO-OXY-2 . . . . . . . . . . . . . . . . . . . 53, 56
Isostim. . . . . . . . . . . . . . . . . . . . . . . . . 95
ISO-TEMP-2. . . . . . . . . . . . . . . . . . . . . 56
IT-1E. . . . . . . . . . . . . . . . . . . . . . . . 40, 51
IT-14 . . . . . . . . . . . . . . . . . . . . . . . . . . 51
IT-18 . . . . . . . . . . . . . . . . . . . . . . . 40, 51
IT-18EXLONG. . . . . . . . . . . . . . . . . . . . 51
IT-21 . . . . . . . . . . . . . . . . . . . . . . . . . . 51
IT-23 . . . . . . . . . . . . . . . . . . . . . . . 40, 51
IV-ECU-H5 . . . . . . . . . . . . . . . . . . . . . 158
IV-ECU-HC . . . . . . . . . . . . . . . . . . . . . 158
IV-ECU-HCP . . . . . . . . . . . . . . . . . . . . 158
IV-ECU-HOC. . . . . . . . . . . . . . . . . . . . 158
J
JE671P. . . . . . . . . . . . . . . . . . . . . . 67, 70
JE671PZ. . . . . . . . . . . . . . . . . . . . . . . . 67
Joystick control. . . . . . . . . . . . . . 136, 142
JUV . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
K
Kelly Hemostatic Forceps . . . . . . . . . . . 23
KG Transducers . . . . . . . . . . . . . . . . . . 86
kidney, perfused. . . . . . . . . . . . . . . . . . . 8
KITE-L . . . . . . . . . . . . . . . . . . . . . . . . 140
KITE-R . . . . . . . . . . . . . . . . . . . . . . . . 140
KITE-TB-L. . . . . . . . . . . . . . . . . . . . . . 140
KITE-TB-R. . . . . . . . . . . . . . . . . . . . . . 140
KITLWCC . . . . . . . . . . . . . . . . . . . . . . 201
KIT-UPUV-STARTUP. . . . . . . . . . . . . . 195
KIT-UPVIS-STARTUP. . . . . . . . . . . . . . 195
KWIK-2 . . . . . . . . . . . . . . . . . . . . . . . . 69
KWIKCAL-2 . . . . . . . . . . . . . . . . . . . . . 69
Kwik-Cast. . . . . . . . . . . . . . . . . . . . . . 100
KWIK-CAST . . . . . . . . . . . . . . . . . . . . 101
Kwik-Cast™ . . . . . . . . . . . . . . . . . . . . 101
KWIKGARD . . . . . . . . . . . . . . . . . . . . 100
Kwik-Gard™. . . . . . . . . . . . . . . . . . . . 100
KWIKGLUE. . . . . . . . . . . . . . . . . . . . . 100
KWIKGUN . . . . . . . . . . . . . . . . . . . . . 100
KWIKH-2. . . . . . . . . . . . . . . . . . . . . . . 69
KWIKMIX. . . . . . . . . . . . . . . . . . . . . . 100
KWIKPOT-2 . . . . . . . . . . . . . . . . . . . . . 69
Kwik-Sil. . . . . . . . . . . . . . . . . . . . . . . 100
KWIK-SIL. . . . . . . . . . . . . . . . . . . . . . 101
Kwik-Sil™. . . . . . . . . . . . . . . . . . . . . . 101
Kwik-Tip™ . . . . . . . . . . . . . . . . . . . . . . 69
KWIKTPP-2 . . . . . . . . . . . . . . . . . . . . . 69
KZ1101. . . . . . . . . . . . . . . . . . . . 43, 117
KZ1106. . . . . . . . . . . . . . . . . . . . . . . . 43
L
Lab Coat. . . . . . . . . . . . . . . . . . . . . . . 102
LABCOAT-L. . . . . . . . . . . . . . . . . . . . . 102
LABCOAT-S. . . . . . . . . . . . . . . . . . . . . 102
LABCOAT-XL. . . . . . . . . . . . . . . . . . . . 102
LABCOAT-XS. . . . . . . . . . . . . . . . . . . . 102
Langendorff . . . . . . . . . . . . . . . . . . . . . . 8
Lapping Film, Alumina; . . . . . . . . . . . 129
Lapping Film, Diamond; . . . . . . . . . . 129
LED light source. . . . . . . . . . . . . . . . . 199
LED-Lite . . . . . . . . . . . . . . . . . . . . . . . 199
LED-LITE. . . . . . . . . . . . . . . . . . . . . . . 199
LED Ringlight. . . . . . . . . . . . . . . . . . . 155
LEDspec. . . . . . . . . . . . . . . . . . . . . . . 192
LEDSpec-2. . . . . . . . . . . . . . . . . . . . . 193
LEDSpec-4. . . . . . . . . . . . . . . . . . . . . 193
Light guides. . . . . . . . . . . . . . . . . . . . 155
Light Sources . . . . . . . . . . . . . . . . . . . 199
Linear Stimulus Isolator. . . . . . . . . . . . 98
Liquid Ion Exchangers . . . . . . . . . . . . . 69
Liquid Waveguide Capillary Cell. . . . . 200
Live cell imaging . . . . . . . . . . . . . . . . 160
LM-5042xx . . . . . . . . . . . . . . . . . . . . 178
LM-504248. . . . . . . . . . . . . . . . . . . . 178
LM-504249. . . . . . . . . . . . . . . . . . . . 178
LM-504250. . . . . . . . . . . . . . . . . . . . 178
LM-504251. . . . . . . . . . . . . . . . . . . . 178
LM-504252. . . . . . . . . . . . . . . . . . . . 178
LM-504253. . . . . . . . . . . . . . . . . . . . 178
LM-504254. . . . . . . . . . . . . . . . . . . . 178
LM-504255. . . . . . . . . . . . . . . . . . . . 178
Item
Page
LM-504256. . . . . . . . . . . . . . . . . . . . 178
LM-504257. . . . . . . . . . . . . . . . . . . . 178
LM-504258. . . . . . . . . . . . . . . . . . . . 178
LM-504259. . . . . . . . . . . . . . . . . . . . 178
LM-504260. . . . . . . . . . . . . . . . . . . . 178
LM-504261. . . . . . . . . . . . . . . . . . . . 178
LM-504262. . . . . . . . . . . . . . . . . . . . 178
LM-504263. . . . . . . . . . . . . . . . . . . . 178
LM-504264. . . . . . . . . . . . . . . . . . . . 178
LM-504265. . . . . . . . . . . . . . . . . . . . 178
LM-504266. . . . . . . . . . . . . . . . . . . . 178
LM-504267. . . . . . . . . . . . . . . . . . . . 178
LM-504268. . . . . . . . . . . . . . . . . . . . 178
LM-504269. . . . . . . . . . . . . . . . . . . . 178
LM-504270. . . . . . . . . . . . . . . . . . . . 178
LM-504271. . . . . . . . . . . . . . . . . . . . 178
LM-504272. . . . . . . . . . . . . . . . . . . . 178
Loupe, Binocular. . . . . . . . . . . . . . . . . . 30
LOUPES . . . . . . . . . . . . . . . . . . . . . . . . 31
Low-Pass Filter. . . . . . . . . . . . . . . . . . . 81
LPF30. . . . . . . . . . . . . . . . . . . . . . . . . . 81
L-shaped sensors. . . . . . . . . . . . . . . . . 56
Luer-to-Tubing Coupler. . . . . . . . . . . . 106
Luer Valve Kit. . . . . . . . . . . . . . . . . . . 106
LUME. . . . . . . . . . . . . . . . . . . . . . . . . . 86
LWCC . . . . . . . . . . . . . . . . . . . . . . . . . 200
LWCC-3050 . . . . . . . . . . . . . . . . 200, 201
LWCC-3100 . . . . . . . . . . . . . . . . 200, 201
LWCC-3250 . . . . . . . . . . . . . . . . 200, 201
LWCC-3500 . . . . . . . . . . . . . . . . 200, 201
LWCC-M-10 . . . . . . . . . . . . . . . . . . . . 202
LWCC-M-50 . . . . . . . . . . . . . . . . . . . . 202
LWCC-M-100. . . . . . . . . . . . . . . . . . . 202
M
M1. . . . . . . . . . . . . . . . . . . . . . . . . . . 145
M1L. . . . . . . . . . . . . . . . . . . . . . . . . . 145
M2. . . . . . . . . . . . . . . . . . . . . . . 141, 143
M-3 . . . . . . . . . . . . . . . . . . 135, 140, 143
M4C. . . . . . . . . 137, 140, 141, 142, 143
M5. . . . . . . . . . . . . . . . . . . 141, 143, 145
M6. . . . . . . . . . . . . . . . . . . . . . . 141, 143
M7. . . . . . . . . . . . . . . . . . . . . . . . . . . 146
M8. . . . . . . . . . . . . . . . . . . . . . . . . . . 145
M9. . . . . . . . . . . . . . . . . . . . . . . . . . . 144
M10. . . . . . . . . . . . . . . . . . . . . . . . . . 144
M10L. . . . . . . . . . . . . . . . . . . . . . . . . 144
M11. . . . . . . . . . . . . . . . . . . . . . . . . . 144
M325. . . . . . . . . . . . . . . . . . . . . . . . . 143
M3301. . . . . . . . . . . . . . . . . . . . . . . . 140
M3301EH . . . . 136, 137, 140, 142, 143
M3301L. . . . . . . . . . . . . . . . . . . . . . . 140
M3301-M3-L. . . . . . . . . . . . . . . . . . . 140
M3301-M3-R. . . . . . . . . . . . . . . . . . . 140
M3301R. . . . . . . . . . . . . . . . . . . . . . . 140
Macro sensors . . . . . . . . . . . . . . . . . . . 53
Magnetic Ball Joint. . . . . . . . . . . . . . . 144
Magnetic Heating Stirrer . . . . . . . . . . 103
Magnetic Holding Devices . . . . . 144, 146
Magnifying Glasses . . . . . . . . . . . . . . . 30
Manometer. . . . . . . . . . . . . . . . . . . . . 187
Manual Micromanipulator . . . . . . . . . 140
Manual Microsyringe Pump. . . . . . . . 187
MAX3820 . . . . . . . . . . . . . . . . . . . . . 105
MAX4020 . . . . . . . . . . . . . . . . . . . . . 105
MB2. . . . . . . . . . . . . . . . . . . . . . . . . . 145
MBMPH5-7 . . . . . . . . . . . . 120, 121, 130
MBS. . . . . . . . . . . . . . . . . . . . . . 124, 129
MCL3-HS6. . . . . . . . . . . . . . . . . . . . . 137
MCL3-SM325. . . . . . . . . . . . . . . . . . . 136
McPherson-Vannas Scissors. . . . . . . . . 24
MD4L. . . . . . . . . . . . . . . . . . . . . . . . . 143
MD4-M3-L. . . . . . . . . . . . . . . . . . . . . 143
MD4-M3-R. . . . . . . . . . . . . . . . . . . . . 143
MD4R. . . . . . . . . . . . . . . . . . . . . . . . . 143
mechanical allodynia . . . . . . . . . . . . . . 36
MechanoCulture. . . . . . . . . . . . . . . . . . 11
megohm meter. . . . . . . . . . . . . . . . . . . 81
MEH1F45. . . . . . . . . . . . . . . . . . . . . . 120
MEH1F45xx. . . . . . . . . . . . . . . . . . . . 121
MEH1R. . . . . . . . . . . . . . . . . . . . . . . . 120
MEH1RF. . . . . . . . . . . . . . . . . . . . . . . 122
MEH1RFxx. . . . . . . . . . . . . . . . . . . . . 121
Item
MEH1Rxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH1S. . . . . . . . . . . . . . . . . . . . . . . . 122
MEH1SF. . . . . . . . . . . . . . . . . . . . . . . 122
MEH1SFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH1Sxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH2R. . . . . . . . . . . . . . . . . . . . . . . . 122
MEH2RF. . . . . . . . . . . . . . . . . . . . . . . 122
MEH2RFW. . . . . . . . . . . . . . . . . . . . . 122
MEH2RFWxx . . . . . . . . . . . . . . . . . . . 121
MEH2RFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH2RW. . . . . . . . . . . . . . . . . . . . . . 122
MEH2RWxx . . . . . . . . . . . . . . . . . . . . 121
MEH2Rxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH2S. . . . . . . . . . . . . . . . . . . . . . . . 122
MEH2SF. . . . . . . . . . . . . . . . . . . . . . . 122
MEH2SFW. . . . . . . . . . . . . . . . . . . . . 122
MEH2SFWxx . . . . . . . . . . . . . . . . . . . 121
MEH2SFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH2SW. . . . . . . . . . . . . . . . . . . . . . 122
MEH2SWxx . . . . . . . . . . . . . . . . . . . . 121
MEH2Sxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH3F45. . . . . . . . . . . . . . . . . . . . . . 122
MEH3F45xx. . . . . . . . . . . . . . . . . . . . 121
MEH3FW45. . . . . . . . . . . . . . . . . . . . 122
MEH3FW45xx. . . . . . . . . . . . . . . . . . 121
MEH3R. . . . . . . . . . . . . . . . . . . . . . . . 122
MEH3RF. . . . . . . . . . . . . . . . . . . . . . . 122
MEH3RFW. . . . . . . . . . . . . . . . . . . . . 122
MEH3RFWxx . . . . . . . . . . . . . . . . . . . 121
MEH3RFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH3RW. . . . . . . . . . . . . . . . . . . . . . 122
MEH3RWxx . . . . . . . . . . . . . . . . . . . . 121
MEH3Rxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH3S. . . . . . . . . . . . . . . . . . . . . . . . 122
MEH3SBW. . . . . . . . . . . . . . . . . . . . . 123
MEH3SBWxx . . . . . . . . . . . . . . . . . . . 121
MEH3SF. . . . . . . . . . . . . . . . . . . . . . . 123
MEH3SFW. . . . . . . . . . . . . . . . . . . . . 123
MEH3SFWxx . . . . . . . . . . . . . . . . . . . 121
MEH3SFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH3SW. . . . . . . . . . . . . . . . . . . . . . 123
MEH3SWxx . . . . . . . . . . . . . . . . . . . . 121
MEH3Sxx. . . . . . . . . . . . . . . . . . . . . . 121
MEH3W45. . . . . . . . . . . . . . . . . . . . . 123
MEH3W45xx. . . . . . . . . . . . . . . . . . . 121
MEH6RF. . . . . . . . . . . . . . . . . . . . . . . 123
MEH6RFW. . . . . . . . . . . . . . . . . . . . . 123
MEH6RFWxx . . . . . . . . . . . . . . . . . . . 121
MEH6RFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH6SF. . . . . . . . . . . . . . . . . . . . . . . 123
MEH6SFW. . . . . . . . . . . . . . . . . . . . . 123
MEH6SFWxx . . . . . . . . . . . . . . . . . . . 121
MEH6SFxx. . . . . . . . . . . . . . . . . . . . . 121
MEH7. . . . . . . . . . . . . . . . . . . . . . . . . 123
MEH7W. . . . . . . . . . . . . . . . . . . . . . . 123
MEH7Wxx . . . . . . . . . . . . . . . . . . . . . 121
MEH7xx. . . . . . . . . . . . . . . . . . . . . . . 121
MEH8. . . . . . . . . . . . . . . . . . . . . . . . . 123
MEH8xx. . . . . . . . . . . . . . . . . . . . . . . 121
MEH145 . . . . . . . . . . . . . . . . . . . . . . 120
MEH145xx. . . . . . . . . . . . . . . . . . . . . 121
MEH345 . . . . . . . . . . . . . . . . . . . . . . 122
MEH345xx. . . . . . . . . . . . . . . . . . . . . 121
MEH900R . . . . . . . . . . . . . . . . . . . . . 123
MEH900Rxx. . . . . . . . . . . . . . . . . . . . 121
MEH900S. . . . . . . . . . . . . . . . . . . . . . 123
MEH900Sxx. . . . . . . . . . . . . . . . . . . . 121
MES. . . . . . . . . . . . . . . . . . . . . . 124, 128
Metal Microelectrodes . . . . . . . . . . . . . 88
MF28G-5. . . . . . . . . . . . . . . . . . . . . . 118
MF28G67-5. . . . . . . . . . . . . . . . . . . . 118
MF34G-5. . . . . . . . . . . . . . . . . . . . . . 118
MF200. . . . . . . . . . . . . . . . . . . . . . . . 124
MF200-1. . . . . . . . . . . . . . . . . . . . . . 125
MF200-2. . . . . . . . . . . . . . . . . . . . . . 125
MF200-H2. . . . . . . . . . . . . . . . . . . . . 125
MF200-H3. . . . . . . . . . . . . . . . . . . . . 125
MF200-H4. . . . . . . . . . . . . . . . . 125, 127
MF200-M1. . . . . . . . . . . . . . . . . . . . . 125
MF200-M2. . . . . . . . . . . . . . . . . . . . . 125
MF200 Microforge. . . . . . . . . . . . . . . 125
MICRO4. . . . . . . . . . . . . . . . . . . . . . . 183
Micro4 Controller. . . . . . . . . . . . 183, 186
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
Page
US: Tel: 941-371-1003 • [email protected]
213
PRODUCT INDEX
II-336T. . . . . . . . . . . . . . . . . . . . . . . . . 39
II-336TG . . . . . . . . . . . . . . . . . . . . . . . 39
II-390. . . . . . . . . . . . . . . . . . . . . . . . . . 39
II-390G . . . . . . . . . . . . . . . . . . . . . . . . 39
II-520MR. . . . . . . . . . . . . . . . . . . . . . . 37
II-600MR. . . . . . . . . . . . . . . . . . . . . . . 38
II-2200 . . . . . . . . . . . . . . . . . . . . . . . . 37
II-2390 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2391 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2392 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2393 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2394 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2395 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2396 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2397 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2400 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-2500 . . . . . . . . . . . . . . . . . . . . . . . . 37
II-2888 . . . . . . . . . . . . . . . . . . . . . . . . 36
II-23931 . . . . . . . . . . . . . . . . . . . . . . . 36
II-MRBP-M. . . . . . . . . . . . . . . . . . . . . . 42
II-MRBP-R . . . . . . . . . . . . . . . . . . . . . . 42
II-PE34. . . . . . . . . . . . . . . . . . . . . . . . . 38
ILS005LT . . . . . . . . . . . . . . . . . . . . . . 183
ILS010LT . . . . . . . . . . . . . . . . . . . . . . 183
ILS025LT . . . . . . . . . . . . . . . . . . . . . . 183
ILS gas-tight syringes. . . . . . . . . . . . . 183
implantable probe . . . . . . . . . . . . . . . . 64
IMPLANTABLE PROBES . . . . . . . . . 40, 51
IN1003 . . . . . . . . . . . . . . . . . . . . . . . 105
Incapacitance Meter. . . . . . . . . . . . . . . 38
Incremental Hot Cold Plate. . . . . . . . . . 38
Indium Wire. . . . . . . . . . . . . . . . . . . . 105
Induction Chambers. . . . . . . . . . . . . . . 35
Infusion Pump . . . . . . . . . . . . . . . . . . 174
Infusion/Withdrawal Pump. . . . . 175, 176
injector. . . . . . . . . . . . . . . . . . . . . . . . 182
inline filter holder. . . . . . . . . . . . . . . . 199
Internal Cannula. . . . . . . . . . . . . . . . . . 49
Intracellular Amplifier. . . . . . . . . . . 73, 74
intracellular injection . . . . . . . . . . . . . 182
INV-101. . . . . . . . . . . . . . . . . . . . . . . 152
Inverted Trinocular Microscope. . . . . . 152
in vitro fertilization. . . . . . . . . . . . . . . 182
IO-KIT . . . . . . . . . . . . . . . . . . . . . . . . 190
ion selective electrodes. . . . . . . . . . . . . 69
iontophoresis. . . . . . . . . . . . . . . . . . . . 81
Iridium Microelectrodes . . . . . . . . . . . . 88
Iris Forceps. . . . . . . . . . . . . . . . . . . . . . 23
Iris Scissors . . . . . . . . . . . . . . . . . . . . . 25
IRM23E01KT. . . . . . . . . . . . . . . . . . . . 89
IRM23E10. . . . . . . . . . . . . . . . . . . . . . 89
IRM23E15. . . . . . . . . . . . . . . . . . . . . . 89
IRM23E20KT. . . . . . . . . . . . . . . . . . . . 89
IRM23E25. . . . . . . . . . . . . . . . . . . . . . 89
IRM23E25KT. . . . . . . . . . . . . . . . . . . . 89
IRM23E30. . . . . . . . . . . . . . . . . . . . . . 89
IRM23E30KT. . . . . . . . . . . . . . . . . . . . 89
ISO2. . . . . . . . . . . . . . . . . . . . . . . . . . . 66
ISO2 Filling Solution . . . . . . . . . . . . . . 66
ISO-80. . . . . . . . . . . . . . . . . . . . . . . . . 80
ISO-80P. . . . . . . . . . . . . . . . . . . . . . . . 80
ISO-H2S-2 . . . . . . . . . . . . . . . . . . . . . . 53
ISO-HPO-2. . . . . . . . . . . . . . . . . . . 53, 56
ISO-HPO-100. . . . . . . . . . . . . . . . . . . . 56
ISO-HPO-100-L . . . . . . . . . . . . . . . . . . 56
Isolated Bioamplifier. . . . . . . . . . . . . . . 80
Isolated Differential Amplifier. . . . . . . . 80
Isolated perfused heart system. . . . . . . . 8
Isolated Perfused
Organ System. . . . . . . . . . . . . . . . . . . . . 8
Isolated working heart system. . . . . . . . 9
ISO-NOP . . . . . . . . . . . . . . . . . . . . 53, 54
ISO-NOP007 . . . . . . . . . . . . . . . . . . . . 55
ISO-NOP30L . . . . . . . . . . . . . . . . . . . . 56
ISO-NOP70L . . . . . . . . . . . . . . . . . . . . 56
ISO-NOP3005 . . . . . . . . . . . . . . . . . . . 55
ISO-NOP3020 . . . . . . . . . . . . . . . . . . . 55
ISO-NOPF100 . . . . . . . . . . . . . . . . . . . 55
ISO-NOPF200 . . . . . . . . . . . . . . . . . . . 55
ISO-NOPF200-L10. . . . . . . . . . . . . . . . 56
ISO-NOPF500 . . . . . . . . . . . . . . . . . . . 55
ISO-NOPNM. . . . . . . . . . . . . . . . . . . . . 54
ISO-NOP Rejuvenator. . . . . . . . . . . . . . 57
Item
PRODUCT INDEX
Item
Page
MicroBeveler. . . . . . . . . . . . . . . . . . . . 129
MicroC . . . . . . . . . . . . . . . . . . . . . . . . . 60
Micro Cannula . . . . . . . . . . . . . . . 43, 117
microCAPSTAR. . . . . . . . . . . . . . . . . . . 41
Micro-Cautery. . . . . . . . . . . . . . . . . 33, 52
Microcentrifuge. . . . . . . . . . . . . . . . . . 104
Microcentrifuge tube, . . . . . . . . . . . . . 104
Micro Coaxial Cables . . . . . . . . . . . . . 105
MICROC-P . . . . . . . . . . . . . . . . . . . . . . 60
Microdialysis Pumps . . . . . . . . . . . . . 176
Microelectrode Beveler. . . . . . . . . . . . 128
Microelectrodes . . . . . . . . . . . . . . . . . . 88
MicroFil . . . . . . . . . . . . . . . . . . . . . . . 118
MicroFlow. . . . . . . . . . . . . . . . . . . . . . . 64
Microfluidic Pump . . . . . . . . . . . . . . . 191
Microforge . . . . . . . . . . . . . . . . . 125, 126
Microforge Application Guide. . . . . . . 124
Microforges, Pullers & Bevelers . . . . . 124
MicroImplant . . . . . . . . . . . . . . . . . . . . 64
Microiontophoresis. . . . . . . . . . . . . . . . 81
Microliter Sample Holder . . . . . . . . . . 202
MicroLWCC. . . . . . . . . . . . . . . . . . . . . 202
Micromanipulator. . . . . . . . 136, 137, 140
Micromanipulators. . . . . . . . . . . . . . . 132
Micrometer, Digital. . . . . . . . . . . . . . . 103
Micrometer Head . . . . . . . . . . . . . . . . 134
Micrometer Slide Micromanipulator. . 143
Micro pH Electrodes. . . . . . . . . . . . . . . 67
Micropipette Beveler. . . . . . . . . . . . . . 128
Micropipette Blanks. . . . . . . . . . . . . . 130
Micropipette Calibration. . . . . . . . . . . 126
Micropipette Holders . . . . . . . . . . . . . 120
Micopipette Puller . . . . . . . . . . . . 130, 131
Micropipette Storage . . . . . . . . . . . . . 116
Micropositioner . . . . . . . . . . . . . . . . . 138
Micropressure System . . . . . . . . . . . . . 90
Microprobe Thermometers. . . . . . . . . . 50
Micro-Reference Electrode. . . . . . . . . . . 68
Micro-Scale Compression. . . . . . . . . . . 11
microscope slides. . . . . . . . . . . . . . . . 164
microscope stage. . . . . . . . . . . . . . . . 141
Microscope Stage Adapter . . . . . . . . . 141
Micro Sensors. . . . . . . . . . . . . . . . . . . . 53
MicroSquisher . . . . . . . . . . . . . . . . . . . 11
MicroTip. . . . . . . . . . . . . . . . . . . . . . . . 64
Microvolume Syringes . . . . . . . . . . . . 183
Millivolt & megohm meter. . . . . . . . . . 81
Miniature Micropositioners. . . . . . . . . 139
Miniature Peristaltic Pump. . . . . . . . . 168
MiniFlow . . . . . . . . . . . . . . . . . . . . . . . 64
MiniFoil . . . . . . . . . . . . . . . . . . . . . . . . 64
Mini-Iris Scissors . . . . . . . . . . . . . . . . . 25
MINISTAR . . . . . . . . . . . . . 168, 193, 201
MiniTip. . . . . . . . . . . . . . . . . . . . . . . . . 64
MityFlex Peristaltic Pump. . . . . . . . . . 168
MITY-KIT . . . . . . . . . . . . . . . . . . . . . . 168
MM1 . . . . . . . . . . . . . . . . . . . . . . . . . 139
MM1-. . . . . . . . . . . . . . . . . . . . . . . . . 139
MM1-ALL. . . . . . . . . . . . . . . . . . . . . . 139
MM1-C. . . . . . . . . . . . . . . . . . . . . . . . 139
MM3 . . . . . . . . . . . . . . . . . . . . . . . . . 139
MM3-3. . . . . . . . . . . . . . . . . . . . . . . . 139
MM3-A. . . . . . . . . . . . . . . . . . . . . . . . 139
MM3-ALL. . . . . . . . . . . . . . . . . . . . . . 139
MM3-C. . . . . . . . . . . . . . . . . . . . . . . . 139
MMJL. . . . . . . . . . . . . . . . . . . . . . . . . 142
MMJR. . . . . . . . . . . . . . . . . . . . . . . . . 142
MMP . . . . . . . . . . . . . . . . . . . . . . . . . 187
MMP-KIT. . . . . . . . . . . . . . . . . . . . . . 187
Mobile Workstations . . . . . . . . . . . . . . 35
Mosquito Hemostatic Forceps. . . . . . . . 23
Motorized Micromanipulator . . . 136, 137
Mouse Kit. . . . . . . . . . . . . . . . . . . . . . . 28
MOUSEKIT. . . . . . . . . . . . . . . . . . . . . . 28
MPH1. . . . . . . . . . . . . . . . . . . . . . . . . 123
MPH1xx. . . . . . . . . . . . . . . . . . . . . . . 121
MPH3. . . . . . . . . . . . . . . . . . . . . . . . . 123
MPH3xx. . . . . . . . . . . . . . . . . . . . . . . 121
MPH4. . . . . . . . . . . . . . . . . . . . . . . . . 123
MPH4xx. . . . . . . . . . . . . . . . . . . . . . . 121
MPH6P. . . . . . . . . . . . . . . . . . . . . . . . 123
MPH6Pxx. . . . . . . . . . . . . . . . . . . . . . 121
MPH6R. . . . . . . . . . . . . . . . . . . 123, 184
214
Item
Page
MPH6Rxx. . . . . . . . . . . . . . . . . . . . . . 121
MPH6S. . . . . . . . . . . . . . . . . . . . 123, 184
MPH6Sxx. . . . . . . . . . . . . . . . . . . . . . 121
MPH8. . . . . . . . . . . . . . . . . . . . . . . . . 135
MPM10 . . . . . . . . . . . . . . . . . . . . . . . 135
MPM20 . . . . . . . . . . . . . . . . . . . . . . . 135
MPS-2 . . . . . . . . . . . . . . . . . . . . . . . . 169
MS314. . . . . . . . . . . . . . . . . . . . . . . . 137
MT-4 . . . . . . . . . . . . . . . . . . . . . . . . . . 51
MT-23/3. . . . . . . . . . . . . . . . . . . . . 40, 51
MT-23/5. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-23/8. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-26/2. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-26/4. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-26/6. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-29/1. . . . . . . . . . . . . . . . . . . . . 40, 51
MT-29/2. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-29/3. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-29/5. . . . . . . . . . . . . . . . . . . . . . . . 51
MT-D . . . . . . . . . . . . . . . . . . . . . . . 40, 51
Multi-Barrel Glass . . . . . . . . . . . . . . . 116
Multi-barrel micropipette coupling. . . 118
Multichannel Perfusion System. . . . . . 169
Multi-Channel Stimulator. . . . . . . . . . . 94
Multipipette Puller. . . . . . . . . . . . . . . 130
muscle hyperalgesia. . . . . . . . . . . . . . . 37
Muscle Research System . . . . . . . . . . . . 4
N
NANOFIL . . . . . . . . . . . . . . . . . . . . . . 188
NANOFIL-100 . . . . . . . . . . . . . . . . . . 188
NanoFil Application Kits. . . . . . . . . . . 190
Nanoliter 2010 . . . . . . . . . . . . . . . . . 186
NANOLITER2010. . . . . . . . . . . . . . . . 186
Nanoliter Injector. . . . . . . . . . . . . . . . 186
NanoSensor . . . . . . . . . . . . . . . . . . . . . 54
Needle Holders. . . . . . . . . . . . . . . . . . . 26
NEEDLE MICROPROBES . . . . . . . . 40, 51
Neely . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Neurotransmitter Detection. . . . . . . . . . 60
NF26BV-2 . . . . . . . . . . . . . . . . . . . . . 188
NF33-36BL . . . . . . . . . . . . . . . . . . . . 189
NF33-36BV . . . . . . . . . . . . . . . . . . . . 189
NF33BL-2. . . . . . . . . . . . . . . . . . . . . . 189
NF33BV-2 . . . . . . . . . . . . . . . . . . . . . 189
NF33FBL-2. . . . . . . . . . . . . . . . . . . . . 189
NF33FBV-2 . . . . . . . . . . . . . . . . . . . . 189
NF34BL-2. . . . . . . . . . . . . . . . . . . . . . 189
NF34BV-2 . . . . . . . . . . . . . . . . . . . . . 189
NF35BL-2. . . . . . . . . . . . . . . . . . . . . . 189
NF35BV-2 . . . . . . . . . . . . . . . . . . . . . 189
NF36BL-2. . . . . . . . . . . . . . . . . . . . . . 189
NF36BV-2 . . . . . . . . . . . . . . . . . . . . . 189
NFGSK-5 . . . . . . . . . . . . . . . . . . . . . . 189
NFINHLD. . . . . . . . . . . . . . . . . . . . . . 189
NFQ34-5 . . . . . . . . . . . . . . . . . . . . . . 189
Nitric Oxide Sensor Guide . . . . . . . . . . 54
Nitric Oxide Sensors. . . . . . . . . . . . . . . 53
NL2010MC4 . . . . . . . . . . . . . . . . . . . 186
NMPH1 . . . . . . . . . . . . . . . . . . . . . . . . 67
NMPH2 . . . . . . . . . . . . . . . . . . . . . . . . 67
NMPH2B . . . . . . . . . . . . . . . . . . . . . . . 67
NMPH3 . . . . . . . . . . . . . . . . . . . . . . . . 67
NMPH3L . . . . . . . . . . . . . . . . . . . . . . . 67
NMPH5 . . . . . . . . . . . . . . . . . . . . . . . . 67
NOCHM-4 . . . . . . . . . . . . . . . . . . . . . . 57
NOCHM-P . . . . . . . . . . . . . . . . . . . . . . 57
noise . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Non-Rotating Micrometer. . . . . . . . . . 134
NOVA. . . . . . . . . . . . . . . . . . . . . . . . . 128
NOVA-186. . . . . . . . . . . . . . . . . . . . . 128
NOVA-Z . . . . . . . . . . . . . . . . . . . . . . . 128
NSA-3 . . . . . . . . . . . . . . . . . . . . . . . . . 57
O
O2 Sensor . . . . . . . . . . . . . . . . . . . . . . 62
Octyl cyanoacrylate . . . . . . . . . . . . . . 102
Omega-Tip-Z . . . . . . . . . . . . . . . . . . . . 81
Omega-Z . . . . . . . . . . . . . . . . . . . . . . 124
OMEGA-Z. . . . . . . . . . . . . . . . . . . . . . . 81
OmniDrill35. . . . . . . . . . . . . . . . . . . . . 29
Oocyte injection. . . . . . . . . . . . . . . . . 186
Open Circuit Potential. . . . . . . . . . . . . . 17
Item
Page
Operating Scissors. . . . . . . . . . . . . . . . 26
Optical Detection Systems . . . . . 193, 195
Optical Detectors . . . . . . . . . . . . . . . . 197
Optical Fibers. . . . . . . . . . . . . . . . . . . 208
Optical Transducer Amp. . . . . . . . . . . . 83
O Ring . . . . . . . . . . . . . . . . . . . . . . . . 128
OXELP . . . . . . . . . . . . . . . . . . . . . . . . . 66
oxygen dipping probe . . . . . . . . . . . . . 64
oxygen electrode . . . . . . . . . . . . . . . . . 66
Oxygen Regulators. . . . . . . . . . . . . . . . 35
oxygen sensing system . . . . . . . . . . . . 62
Oxygen Sensors. . . . . . . . . . . . . . . 53, 56
OXY-MICRO-AOT . . . . . . . . . . . . . . . . . 64
OXY-MINI-AOT. . . . . . . . . . . . . . . . . . . 64
P
P-5-10. . . . . . . . . . . . . . . . . . . . . . . . 130
P-7-10. . . . . . . . . . . . . . . . . . . . . . . . 130
Parallel Rail . . . . . . . . . . . . . . . . . . . . . 48
Patch Clamp. . . . . . . . . . . . . . . . . . . . 115
Paw Pressure Meter. . . . . . . . . . . . . . . 37
Paw Volume Meter. . . . . . . . . . . . . . . . 37
PB150F-4. . . . . . . . . . . . . . . . . . . . . . 116
PB150F-6. . . . . . . . . . . . . . . . . . . . . . 116
perfusion chambers . . . . . . . . . . . . . . . 18
Perfusion System . . . . . . . . . . . . . . . . 169
PERIPRO-2HS . . . . . . . . . . . . . . . . . . 167
PERIPRO-4HS . . . . . . . . . . . . . . . . . . 167
PERIPRO-4LS. . . . . . . . . . . 167, 193, 201
PERIPRO-8LS. . . . . . . . . . . . . . . . . . . 167
Peristaltic pump. . . . . . . . . . . . . 166, 168
Peri-Star Pro. . . . . . . . . . . . . . . . . . . . 166
PE Tubing, Flexible. . . . . . . . . . . . . . . . 49
PG10150-4. . . . . . . . . . . . . . . . . . . . 115
PG10165-4. . . . . . . . . . . . . . . . . . . . 115
PG52151-4. . . . . . . . . . . . . . . . . . . . 115
PG52165-4. . . . . . . . . . . . . . . . . . . . 115
pH electrode. . . . . . . . . . . . . . . . . . 67, 70
pH Meter . . . . . . . . . . . . . . . . . . . . 65, 70
pH Meter, Fiber Optic. . . . . . . . . . . . . . 65
pH mini sensors. . . . . . . . . . . . . . . . . . 65
PH-OPTICA-MICRO . . . . . . . . . . . . . . . 65
PH-OPTICA-MINI. . . . . . . . . . . . . . . . . 65
pHOptica™. . . . . . . . . . . . . . . . . . . . . . 65
Photocell. . . . . . . . . . . . . . . . . . . . . . . . 86
PH-SILVER . . . . . . . . . . . . . . . . . . . . . . 67
PI-305A. . . . . . . . . . . . . . . . . . . . . . . 130
PicoNozzle Kit. . . . . . . . . . . . . . . . . . . 184
PID. . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Piezo Micromanipulator . . . . . . . . . . . 133
Piezo-Translator. . . . . . . . . . . . . . . . . 135
Piggyback. . . . . . . . . . . . . . . . . . . . . . 116
PI-MC2010 . . . . . . . . . . . . . . . . . . 33, 52
Pipette Tips . . . . . . . . . . . . . . . . . . . . 181
Pipetters. . . . . . . . . . . . . . . 178, 179, 180
Planachromatic Objective. . . . . . . . . . 149
Plantar Test . . . . . . . . . . . . . . . . . . . . . 39
Platinum-iridium Microelectrodes. . . . . 88
Platinum / Iridium Teflon Wire. . . . . . 105
Platinum / Iridium Wire. . . . . . . . . . . 105
Platinum Wire . . . . . . . . . . . . . . . . . . 105
Plethysmometer. . . . . . . . . . . . . . . . . . 37
PM01. . . . . . . . . . . . . . . . . . . . . . . . . 187
PM01D. . . . . . . . . . . . . . . . . . . . . . . . 187
PM01R. . . . . . . . . . . . . . . . . . . . . . . . 187
PM5. . . . . . . . . . . . . . . . . . . . . . 135, 137
PM6. . . . . . . . . . . . . . . . . . . . . . . . . . 135
PM7. . . . . . . . . . . . . . . . . . . . . . . . . . 135
PM015. . . . . . . . . . . . . . . . . . . . . . . . 187
PM015D . . . . . . . . . . . . . . . . . . . . . . 187
PM015R. . . . . . . . . . . . . . . . . . . . . . . 187
PM100. . . . . . . . . . . . . . . . . . . . . . . . 187
PM100D . . . . . . . . . . . . . . . . . . . . . . 187
PM100R. . . . . . . . . . . . . . . . . . . . . . . 187
PMP-107. . . . . . . . . . . . . . . . . . 124, 130
PMP-107Z. . . . . . . . . . . . . . . . . . . . . 130
PM Series. . . . . . . . . . . . . . . . . . . . . . 187
PNEU01. . . . . . . . . . . . . . . . . . . . . . . . 86
PNEU05. . . . . . . . . . . . . . . . . . . . . . . . 86
PNEU15. . . . . . . . . . . . . . . . . . . . . . . . 86
Pneumatic PicoPump . . . . . . . . . . . . . 184
PNEU transducers . . . . . . . . . . . . . . . . 86
Polishing Patch Pipettes. . . . . . . . . . . 127
Item
Page
PolyFil . . . . . . . . . . . . . . . . . . . . . . . . 118
Post . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Post Stand . . . . . . . . . . . . . . . . . . . . . 151
Potassium Electrode. . . . . . . . . . . . . . . 69
Potentiostat . . . . . . . . . . . . . . . . . . . . . 60
POWERCORDS. . . . . . . . . . . . . . . . . . 109
Power Frame Enclosure. . . . . . . . . . . . . 82
Preamplifier . . . . . . . . . . . . . . . . . . . . . 17
Precision Current Sources. . . . . . . . . . . 96
Precision SurgioScope . . . . . . . . . . . . 154
Pre-polarizer. . . . . . . . . . . . . . . . . . . . . 57
Pressure Ejection Kit. . . . . . . . . . . . . . 130
Pressure Manometer . . . . . . . . . . . . . 187
Pressure Pod . . . . . . . . . . . . . . . . . . . . 90
Pressure Sensor. . . . . . . . . . . . . . . . . . 86
Programmable Syringe Pump. . . . . . . 177
ProGuide Position/Holder. . . . . . . . . . . 70
PSMB5. . . . . . . . . . . . . . . . . . . . . . . . 154
PSMT5. . . . . . . . . . . . . . . . . . . . . . . . 154
PT0110 . . . . . . . . . . . . . . . . . . . . . . . 105
PT0203 . . . . . . . . . . . . . . . . . . . . . . . 105
PT0402 . . . . . . . . . . . . . . . . . . . . . . . 105
PTM3CC02INS. . . . . . . . . . . . . . . . . . . 89
PTM23CC001NON. . . . . . . . . . . . . . . . 89
PTP201 . . . . . . . . . . . . . . . . . . . . . . . 105
PTP401 . . . . . . . . . . . . . . . . . . . . . . . 105
PTP406 . . . . . . . . . . . . . . . . . . . . . . . 105
PTPxxx. . . . . . . . . . . . . . . . . . . . . . . . 105
PTT0110 . . . . . . . . . . . . . . . . . . . . . . 105
PTT0203 . . . . . . . . . . . . . . . . . . . . . . 105
PTTxxxx. . . . . . . . . . . . . . . . . . . . . . . 105
PTxxxx. . . . . . . . . . . . . . . . . . . . . . . . 105
PUL-1000 . . . . . . . . . . . . . . . . . . . . . . 131
Puller, Micopipette. . . . . . . . . . . . . . . . 131
Puller, Programmable. . . . . . . . . . . . . 130
pulse generator . . . . . . . . . . . . . . . 93, 94
Pulsemaster™Multi-Channel Stimulator. 94
Pumps & Fluid Handling . . . . . . . . . . 165
Push-Pull Pump. . . . . . . . . . . . . . . . . 175
PV820. . . . . . . . . . . . . . . . . . . . . . . . 185
PV830. . . . . . . . . . . . . . . . . . . . . . . . 184
PZMIII . . . . . . . . . . . . . . . . . . . . . . . . 150
PZMIII-AAC . . . . . . . . . . . . . . . . . . . . 150
PZMIII-BS. . . . . . . . . . . . . . . . . . . . . . 150
PZMIV . . . . . . . . . . . . . . . . . . . . . . . . 148
PZMIV-BS. . . . . . . . . . . . . . . . . . . . . . 148
PZMTIII . . . . . . . . . . . . . . . . . . . . . . . 150
PZMTIII-AAC. . . . . . . . . . . . . . . . . . . . 150
PZMTIII-BS. . . . . . . . . . . . . . . . . . . . . 150
PZMTIII-BS-DIG50. . . . . . . . . . . . . . . 150
PZMTIII-BS-LWD-DIG50. . . . . . . . . . . 150
PZMTIII-DIG50. . . . . . . . . . . . . . . . . . 150
PZMTIV . . . . . . . . . . . . . . . . . . . . . . . 148
PZMTIV-BCS; PZMTIV-BS. . . . . . . . . . 148
PZMTIV-BS-DIG50. . . . . . . . . . . . . . . 148
PZMTIV-BS-LWD-DIG50. . . . . . . . . . . 148
PZMTIV-DIG50. . . . . . . . . . . . . . . . . . 148
Q
QFT1 . . . . . . . . . . . . . . . . . . . . . . . . . 207
Quartz Cuvettes . . . . . . . . . . . . . . . . . 204
Quattro. . . . . . . . . . . . . . . . . . . . . . . . . 36
quiet. . . . . . . . . . . . . . . . . . . . . . . . . . . 40
R
R-8-8-WPI01. . . . . . . . . . . . . . . 149, 155
Rackmounting Hardware . . . . . . . . . . 104
Randall Selitto Paw Pressure Meter . . . 37
RBMA-200C. . . . . . . . . . . . . . . . . . . . . 20
RBMA-200S. . . . . . . . . . . . . . . . . . . . . 20
RBMA-300C. . . . . . . . . . . . . . . . . . . . . 20
RBMA-300S. . . . . . . . . . . . . . . . . . . . . 20
RBMA-600C. . . . . . . . . . . . . . . . . . . . . 20
RBMA-600S. . . . . . . . . . . . . . . . . . . . . 20
RBMS-200C. . . . . . . . . . . . . . . . . . . . . 20
RBMS-200S. . . . . . . . . . . . . . . . . . . . . 20
RBMS-300C. . . . . . . . . . . . . . . . . . . . . 20
RBMS-300S. . . . . . . . . . . . . . . . . . . . . 20
RBMS-600C. . . . . . . . . . . . . . . . . . . . . 20
RBMS-600S. . . . . . . . . . . . . . . . . . . . . 20
RC1 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC1T . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC2 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
US: Tel: 941-371-1003 • [email protected]
Item
Page
RC2F . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC3 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC4 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC5 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
RC6 . . . . . . . . . . . . . . . . . . . . . . . . . . 119
RECTAL PROBES. . . . . . . . . . . . . . . 40, 51
rectal temperature probe . . . . . . . . . . . 40
Rectangular Base. . . . . . . . . . . . . . . . 110
Reference Electrodes. . . . . . . . . . . . . . . 68
Reflex Clip . . . . . . . . . . . . . . . . . . . . . . 27
Reflex Clip Applier. . . . . . . . . . . . . . . . 27
REG2. . . . . . . . . . . . . . . . . . . . . . . . . . 180
REG10. . . . . . . . . . . . . . . . . . . . . . . . . 180
REG20. . . . . . . . . . . . . . . . . . . . . . . . . 180
REG50. . . . . . . . . . . . . . . . . . . . . . . . . 180
REG100. . . . . . . . . . . . . . . . . . . . . . . . 180
REG200. . . . . . . . . . . . . . . . . . . . . . . . 180
REG1000. . . . . . . . . . . . . . . . . . . . . . . 180
REG5K. . . . . . . . . . . . . . . . . . . . . . . . . 180
REG10K. . . . . . . . . . . . . . . . . . . . . . . . 180
REGS5. . . . . . . . . . . . . . . . . . . . . . . . . 180
REGS6. . . . . . . . . . . . . . . . . . . . . . . . . 180
REGS7. . . . . . . . . . . . . . . . . . . . . . . . . 180
Regal Pipetters. . . . . . . . . . . . . . . . . . . 180
REMS-24. . . . . . . . . . . . . . . . . . . . . . . 16
REMS-96. . . . . . . . . . . . . . . . . . . . . . . 16
REMS AutoSampler . . . . . . . . . . . . . . . 16
Replacement Electrode Holder . . . . . . 140
Replacement Filaments. . . . . . . . . . . . . 131
Replacement Needles. . . . . . . . . . . . . 183
Replacement Sleeves . . . . . . . . . . . . . . 53
Replacement Tubing. . . . . . . . . . . . . . 167
RET-2. . . . . . . . . . . . . . . . . . . . . . . 40, 51
RET-3. . . . . . . . . . . . . . . . . . . . . . . . . . 51
Retractors. . . . . . . . . . . . . . . . . . . . . . . 27
Ring Light. . . . . . . . . . . . . . . . . . . . . . 155
Ring Light Adapter. . . . . . . . . . . . . . . 155
Ring Light Guide . . . . . . . . . . . . . . . . 155
RN001. . . . . . . . . . . . . . . . . . . . . . . . 183
RN0005. . . . . . . . . . . . . . . . . . . . . . . 183
RN010. . . . . . . . . . . . . . . . . . . . . . . . 183
RN025. . . . . . . . . . . . . . . . . . . . . . . . 183
Rodent Brain Matrices . . . . . . . . . . . . . 20
Roger Wirecutting Scissors. . . . . . . . . 103
RPE-KIT. . . . . . . . . . . . . . . . . . . . . . . 190
RTD sensor. . . . . . . . . . . . . . . . . . . . . . 40
RTV Coating. . . . . . . . . . . . . . . . . . . . 101
RTV Prime Coat,. . . . . . . . . . . . . . . . . 101
RTV Sealant. . . . . . . . . . . . . . . . . . . . 101
194
202
50
27
27
24
100
103
68
53
116
116
191
191
191
191
191
191
191
191
183
183
183
183
183
183
183
183
183
183
84
83
Page
SI-BMFA. . . . . . . . . . . . . . . . . . . . . . . . 82
SI-BRIDGE80. . . . . . . . . . . . . . . . . . . . 83
SI-CTS200 . . . . . . . . . . . . . . . . . . . . . . . 3
SI-DAM800 . . . . . . . . . . . . . . . . . . . . . 84
Signal Conditioning Amplifiers. . . . . . . 82
Signal Generator . . . . . . . . . . . . . . . . . 93
SI-HTB2. . . . . . . . . . . . . . . . . . . . . . . . . 5
SI-HTB2M. . . . . . . . . . . . . . . . . . . . . . . . 5
SI-HTB4. . . . . . . . . . . . . . . . . . . . . . . . . 5
SI-IPOKIDNEY . . . . . . . . . . . . . . . . . . . . 8
SI-KG2. . . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KG2A. . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KG4. . . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KG4A. . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KG7A. . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KG7B. . . . . . . . . . . . . . . . . . . . . . . . 86
SI-KGxx . . . . . . . . . . . . . . . . . . . . . . . . 86
SI-LANG1. . . . . . . . . . . . . . . . . . . . . . . . 8
SI-LANG2. . . . . . . . . . . . . . . . . . . . . . . . 8
SI-LANGC. . . . . . . . . . . . . . . . . . . . . . . . 7
SI-LANGWH. . . . . . . . . . . . . . . . . . . . . . 9
SI-LF-05-01-IPO. . . . . . . . . . . . . . . . . . . 9
SILFLEX-2. . . . . . . . . . . . . . . . . . . . . . 189
Silicone RTV. . . . . . . . . . . . . . . . . . . . 101
Silicone Tubing. . . . . . . . . . . . . . 167, 168
Silver Epoxy. . . . . . . . . . . . . . . . . . . . 101
Silver Wire. . . . . . . . . . . . . . . . . . . . . 105
SI-MKB. . . . . . . . . . . . . . . . . . . . . . . . . . 4
SI-MKBM-OXY. . . . . . . . . . . . . . . . . . . . 4
SI-MKB-OXY . . . . . . . . . . . . . . . . . . . . . 4
Single Barrel. . . . . . . . . . . . . . . . 114, 131
SI-SEN07RTH2. . . . . . . . . . . . . . . . 7, 8, 9
SI-SEN12AM4E-B. . . . . . . . . . . . . . 7, 8, 9
SI-SEN12AM4E-U . . . . . . . . . . . . . 7, 8, 9
SI-SEN13MAP . . . . . . . . . . . . . . . . 7, 8, 9
SI-TCM2. . . . . . . . . . . . . . . . . . . . . . . . 85
SM325. . . . . . . . . . . . . . . . . . . . . . . . 136
SM325-M. . . . . . . . . . . . . . . . . . . . . . 136
SNAP. . . . . . . . . . . . . . . . . . . . . . . . . . 57
SNAP25. . . . . . . . . . . . . . . . . . . . . . . . 57
SNAP50. . . . . . . . . . . . . . . 54, 55, 57, 59
SNAP100. . . . . . . . . . . . . . . . . . . . . . . 57
S-nitrosoglutathione. . . . . . . . . . . . . . . 57
S-Nitroso-N-acetyl-D,L-penicillamine . . 57
SN-PCZ-50L. . . . . . . . . . . . . . . . . . . . 133
SN-PCZ-50R. . . . . . . . . . . . . . . . . . . . 133
SN-PZ-50. . . . . . . . . . . . . . . . . . . . . . 133
SN-PZ-50L. . . . . . . . . . . . . . . . . . . . . 133
SN-PZ-50R. . . . . . . . . . . . . . . . . . . . . 133
SP100i. . . . . . . . . . . . . . . . . . . . . . . . 174
SP100iZ. . . . . . . . . . . . . . . . . . . . . . . 174
SP101i. . . . . . . . . . . . . . . . . . . . . . . . 176
SP101iZ. . . . . . . . . . . . . . . . . . . . . . . 176
SP120p . . . . . . . . . . . . . . . . . . . . . . . 176
SP120pZ . . . . . . . . . . . . . . . . . . . . . . 176
SP200i. . . . . . . . . . . . . . . . . . . . . . . . 176
SP200iZ. . . . . . . . . . . . . . . . . . . . . . . 176
SP210c . . . . . . . . . . . . . . . . . . . . . . . 175
SP210cZ. . . . . . . . . . . . . . . . . . . . . . . 175
SP210iw . . . . . . . . . . . . . . . . . . . . . . 175
SP210iwZ . . . . . . . . . . . . . . . . . . . . . 175
SP220i. . . . . . . . . . . . . . . . . . . . . . . . 174
SP220iZ. . . . . . . . . . . . . . . . . . . . . . . 174
SP230iw . . . . . . . . . . . . . . . . . . . . . . 176
SP230iwZ . . . . . . . . . . . . . . . . . . . . . 176
SP250i. . . . . . . . . . . . . . . . . . . . . . . . 176
SP250iZ. . . . . . . . . . . . . . . . . . . . . . . 176
SP260p . . . . . . . . . . . . . . . . . . . . . . . 175
SP260pZ . . . . . . . . . . . . . . . . . . . . . . 175
SpectraView. . . . . . . . . . . . . . . . . . . . 197
spectrometer. . . . . . . . . . . . . . . . . . . . 196
SPLG100 . . . . . . . . . . . . . . . . . . . . . . 170
SPLG101 . . . . . . . . . . . . . . . . . . . . . . 171
SPLG110 . . . . . . . . . . . . . . . . . . . . . . 171
SPLG200 . . . . . . . . . . . . . . . . . . . . . . 173
SPLG210 . . . . . . . . . . . . . . . . . . . . . . 172
SPLG212 . . . . . . . . . . . . . . . . . . . . . . 172
SPLG270 . . . . . . . . . . . . . . . . . . . . . . 172
SPLG272 . . . . . . . . . . . . . . . . . . . . . . 172
SS316xx . . . . . . . . . . . . . . . . . . . . . . 105
SS31614 . . . . . . . . . . . . . . . . . . . . . . 105
SSM33A20KT . . . . . . . . . . . . . . . . . . . 89
SSM33A70. . . . . . . . . . . . . . . . . . . . . . 89
Item
Page
SSM33A120 . . . . . . . . . . . . . . . . . . . . 89
SST30407-50 . . . . . . . . . . . . . . . . . . 105
SSTxxxxx . . . . . . . . . . . . . . . . . . . . . . 105
Stainless Steel Rods. . . . . . . . . . . . . . 110
Stainless Steel Wire . . . . . . . . . . . . . . 105
Stand for Pipetters . . . . . . . . . . . 179, 180
Steel Base Plate . . . . . . . . . . . . . . . . . 146
Stereotaxic . . . . . . . . . . . . . . . . . . . . . . 44
Stereotaxic Accessories. . . . . . . . . . . . . 46
Stereotaxic Frame. . . . . . . . . . . . . . . . . 44
Stereo Zoom Microscope. . . . . . . 148, 150
Sterilizer. . . . . . . . . . . . . . . . . . . . . . . . 32
Sterilizing Trays . . . . . . . . . . . . . . . . . . 32
STEV. . . . . . . . . . . . . . . . . . . . . . . . . . 158
stimulator. . . . . . . . . . . . . . . . . . . . 93, 94
Stimulator, Digital . . . . . . . . . . . . . . . . 99
Stimulator/Isolator. . . . . . . . . . . . . . . . 95
Stimulus Isolator . . . . . . . . 96, 97, 98, 99
Stirrer. . . . . . . . . . . . . . . . . . . . . . . . . 103
STM3. . . . . . . . . . . . . . . . . . . . . . . . . 137
Stopcock. . . . . . . . . . . . . . . . . . . . . . . 107
STX2 . . . . . . . . . . . . . . . . . . . . . . . . . . 13
STX3 . . . . . . . . . . . . . . . . . . . . . . . . . . 13
STX100 . . . . . . . . . . . . . . . . . . . . . . . . 14
STX100C . . . . . . . . . . . . . . . . . . . . . . . 14
STX100C96. . . . . . . . . . . . . . . . . . . . . 14
STX100F . . . . . . . . . . . . . . . . . . . . . . . 14
STX100M. . . . . . . . . . . . . . . . . . . . . . . 14
Sub-microliter injection. . . . . . . . . . . . 188
Super Adhesive . . . . . . . . . . . . . . . . . 102
SuperCut. . . . . . . . . . . . . . . . . . . . . . . . 25
SUPER-Dri-Ref. . . . . . . . . . . . . . . . . . . 68
Sure-Seal Chambers. . . . . . . . . . . . . . . 35
SurgioScope. . . . . . . . . . . . . . . . . . . . 154
SYLG184 . . . . . . . . . . . . . . . . . . . . . . 100
Sylgard. . . . . . . . . . . . . . . . . . . . . . . . 102
Syringe Pump. . . . . . . . . . . . . . . 170, 172
Syringe Pumps. . . . . . . . . . . . . . 174, 177
Syringes with Luers . . . . . . . . . . . . . . 183
Syringes with Needles . . . . . . . . . . . . 183
SYS-121. . . . . . . . . . . . . . . . . . . . . . . . 81
SYS-260. . . . . . . . . . . . . . . . . . . . . . . . 81
SYS-705. . . . . . . . . . . . . . . . . . . . . . . . 73
SYS-773. . . . . . . . . . . . . . . . . . . . . . . . 75
SYS-900A. . . . . . . . . . . . . . . . . . . . . . . 90
SYS-A300. . . . . . . . . . . . . . . . . . . . . . . 94
SYS-A310. . . . . . . . . . . . . . . . . . . . . . . 93
SYS-A320D . . . . . . . . . . . . . . . . . . . . . 95
SYS-A320R. . . . . . . . . . . . . . . . . . . . . . 95
SYS-A362. . . . . . . . . . . . . . . . . 95, 96, 98
SYS-A365D . . . . . . . . . . . . . . . . . . . . . 96
SYS-A365R. . . . . . . . . . . . . . . . . . . . . . 96
SYS-A382. . . . . . . . . . . . . . . . . . . . . . . 97
SYS-A385R. . . . . . . . . . . . . . . . . . . . . . 97
SYS-A385RC . . . . . . . . . . . . . . . . . . . . 97
SYS-ABM . . . . . . . . . . . . . . . . . . . . . . . 81
SYS-BP1. . . . . . . . . . . . . . . . . . . . . . . . 43
SYS-DAM50. . . . . . . . . . . . . . . . . . . . . 79
SYS-DAM80. . . . . . . . . . . . . . . . . . . . . 79
SYS-EVC4000. . . . . . . . . . . . . . . . . . . . 17
SYS-ISO2. . . . . . . . . . . . . . . . . . . . . . . 66
SYS-LPF30. . . . . . . . . . . . . . . . . . . . . . 81
SYS-MICRO4 . . . . . . . . . . . . . . . . . . . 183
SYS-MICROC . . . . . . . . . . . . . . . . . . . . 60
SYS-MS314 . . . . . . . . . . . . . . . . . . . . 137
SYS-OMEGAZ. . . . . . . . . . . . . . . . 81, 128
SYS-PM01D. . . . . . . . . . . . . . . . . . . . 187
SYS-PM01R . . . . . . . . . . . . . . . . . . . . 187
SYS-PM015D. . . . . . . . . . . . . . . . . . . 187
SYS-PM015R. . . . . . . . . . . . . . . . . . . 187
SYS-PM100D. . . . . . . . . . . . . . . . . . . 187
SYS-PM100R. . . . . . . . . . . . . . . . . . . 187
SYS-PV820. . . . . . . . . . . . . . . . . . . . . 184
SYS-PV830. . . . . . . . . . . . . . . . . . . . . 184
SYS-REMS . . . . . . . . . . . . . . . . . . . . . . 16
SYS-TBM4M. . . . . . . . . . . . . . . . . . . . . 91
T
Tail Flick Test . . . . . . . . . . . . . . . . . . . .
TAXIC-600. . . . . . . . . . . . . . . . . . . . . .
TAXIC-603. . . . . . . . . . . . . . . . . . . . . .
TAXIC-650. . . . . . . . . . . . . . . . . . . . . .
TAXIC-653. . . . . . . . . . . . . . . . . . . . . .
39
44
44
44
44
Item
TB-1. . . . . . . . . . . . . . . . . . . . . . . . . . 140
TBM4M . . . . . . . . . . . . . . . . . . . . . . . . 91
TBR1025. . . . . . . . . . . . . . . . . . . . 58, 59
TBR4100. . . . . . . . . . . . . . . . . . . . . . . 59
TBR4100-416. . . . . . . . . . . . . . . . . . . 59
TBR4100-424T. . . . . . . . . . . . . . . . . . 59
TBS. . . . . . . . . . . . . . . . . . . . . . 137, 141
TCJ3050. . . . . . . . . . . . . . . . . . . . . . . 105
TCK3050. . . . . . . . . . . . . . . . . . . . . . 105
TEER measurement . . . . . . . . . 13, 15, 16
Temperature Controller. . . . . . . . . . . . . 40
Temperature Control Unit . . . . . . . . . . . 85
temperature probe . . . . . . . . . . . . . . . 160
Temperature Probes . . . . . . . . . . . . . . . 50
Temperature Sensor . . . . . . . . . . . . . . . 56
TGW0515 . . . . . . . . . . . . . . . . . . . . . 105
TGW1510 . . . . . . . . . . . . . . . . . . . . . 105
TGWxxxx . . . . . . . . . . . . . . . . . . . . . . 105
Thermocouple Wire . . . . . . . . . . . . . . 105
Thin Wall. . . . . . . . . . . . . . . . . . 114, 131
Three-barrel glass . . . . . . . . . . . . . . . 116
TI4024. . . . . . . . . . . . . . . . . . . . . . . . 105
TidasDAQ. . . . . . . . . . . . . . . . . . . . . . 197
TIDASI . . . . . . . . . . . . . . . . . . . . . . . . 196
Tilt Base. . . . . . . . . . . . . . . . . . . 137, 141
Tilt Base Assembly. . . . . . . . . . . . . . . 129
TIP1TW1. . . . . . . . . . . . . . . . . . . . . . 117
TIP01TW1F . . . . . . . . . . . . . . . . . . . . 117
TIP01TW1F-L. . . . . . . . . . . . . . . . . . . 117
TIP1TW1-L. . . . . . . . . . . . . . . . . . . . . 117
TIP2TW1. . . . . . . . . . . . . . . . . . . . . . 117
TIP02TW1F . . . . . . . . . . . . . . . . . . . . 117
TIP02TW1F-L. . . . . . . . . . . . . . . . . . . 117
TIP2TW1-L. . . . . . . . . . . . . . . . . . . . . 117
TIP03TW1F . . . . . . . . . . . . . . . . . . . . 117
TIP03TW1F-L. . . . . . . . . . . . . . . . . . . 117
TIP04TW1F . . . . . . . . . . . . . . . . . . . . 117
TIP04TW1F-L. . . . . . . . . . . . . . . . . . . 117
TIP5TW1. . . . . . . . . . . . . . . . . . . . . . 117
TIP05TW1F . . . . . . . . . . . . . . . . . . . . 117
TIP05TW1F-L. . . . . . . . . . . . . . . . . . . 117
TIP5TW1-L. . . . . . . . . . . . . . . . . . . . . 117
TIP5TW1LS01 . . . . . . . . . . . . . . . . . . 117
TIP5TW1LS02 . . . . . . . . . . . . . . . . . . 117
TIP10TW1. . . . . . . . . . . . . . . . . . . . . 117
TIP10TW1-L. . . . . . . . . . . . . . . . . . . . 117
TIP10TW1LS01. . . . . . . . . . . . . . . . . 117
TIP10TW1LS02. . . . . . . . . . . . . . . . . 117
TIP10XV119 . . . . . . . . . . . . . . . 117, 186
TIP30TW1. . . . . . . . . . . . . . . . . . . . . 117
TIP30TW1-L. . . . . . . . . . . . . . . . . . . . 117
TIP30TW1LS01. . . . . . . . . . . . . . . . . 117
TIP30TW1LS02. . . . . . . . . . . . . . . . . 117
TIPCA. . . . . . . . . . . . . . . . . . . . . . . . . . 69
TIPH. . . . . . . . . . . . . . . . . . . . . . . . . . . 69
TIPK. . . . . . . . . . . . . . . . . . . . . . . . . . . 69
TIPMIX01-05. . . . . . . . . . . . . . . . . . . 117
TIPMIX01-05-L . . . . . . . . . . . . . . . . . 117
TIPMIX05-10. . . . . . . . . . . . . . . . . . . 117
TIPMIX05-10-L . . . . . . . . . . . . . . . . . 117
TIPTPP. . . . . . . . . . . . . . . . . . . . . . . . . 69
Tissue Bath for Liver. . . . . . . . . . . . . . . . 9
Tissue Mounts . . . . . . . . . . . . . . . . . . . . 4
Titanium wire. . . . . . . . . . . . . . . . . . . 105
TIxxxx . . . . . . . . . . . . . . . . . . . . . . . . 105
TM31/33Axx. . . . . . . . . . . . . . . . . . . . 89
TM31/33AxxKT. . . . . . . . . . . . . . . . . . 89
TM31A10. . . . . . . . . . . . . . . . . . . . . . . 89
TM31A10KT . . . . . . . . . . . . . . . . . . . . 89
TM31A20. . . . . . . . . . . . . . . . . . . . . . . 89
TM31C05. . . . . . . . . . . . . . . . . . . . . . . 89
TM33A05. . . . . . . . . . . . . . . . . . . . . . . 89
TM33A10. . . . . . . . . . . . . . . . . . . . . . . 89
TM33A10KT . . . . . . . . . . . . . . . . . . . . 89
TM33A20. . . . . . . . . . . . . . . . . . . . . . . 89
TM33B01. . . . . . . . . . . . . . . . . . . . . . . 89
TM33B01KT . . . . . . . . . . . . . . . . . . . . 89
TM33B05. . . . . . . . . . . . . . . . . . . . . . . 89
TM33B05KT . . . . . . . . . . . . . . . . . . . . 89
TM33B10. . . . . . . . . . . . . . . . . . . . . . . 89
TM33B10KT . . . . . . . . . . . . . . . . . . . . 89
TM33B20. . . . . . . . . . . . . . . . . . . . . . . 89
TM33BxxKT. . . . . . . . . . . . . . . . . . . . . 89
World Precision Instruments
UK: Tel: +44 (0)1462 424700 • [email protected]
www.wpiinc.com
Germany: Tel: +49 (0)30-6188845 • [email protected]
Page
US: Tel: 941-371-1003 • [email protected]
215
PRODUCT INDEX
S
sample cell. . . . . . . . . . . . . . . . . . . . .
Sample holder, Microliter. . . . . . . . . .
SBT-5. . . . . . . . . . . . . . . . . . . . . . . . . .
Scalpel Blades . . . . . . . . . . . . . . . . . . .
Scalpel Handle. . . . . . . . . . . . . . . . . . .
Scissors . . . . . . . . . . . . . . . . . . . . . . . .
Scotch-Weld. . . . . . . . . . . . . . . . . . . .
Screwdriver Set . . . . . . . . . . . . . . . . .
SDR2. . . . . . . . . . . . . . . . . . . . . . . . . .
Sensor Guide. . . . . . . . . . . . . . . . . . . .
Septum Theta. . . . . . . . . . . . . . . . . . .
Seven-barrel glass . . . . . . . . . . . . . . .
SF-MFP. . . . . . . . . . . . . . . . . . . . . . . .
SF-QC100 . . . . . . . . . . . . . . . . . . . . .
SF-QC101 . . . . . . . . . . . . . . . . . . . . .
SF-QC102 . . . . . . . . . . . . . . . . . . . . .
SF-QC103 . . . . . . . . . . . . . . . . . . . . .
SF-QC104 . . . . . . . . . . . . . . . . . . . . .
SF-QC105 . . . . . . . . . . . . . . . . . . . . .
SF-QCTUBING . . . . . . . . . . . . . . . . . .
SGE001RN. . . . . . . . . . . . . . . . . . . . .
SGE0005RN . . . . . . . . . . . . . . . . . . .
SGE010RNS. . . . . . . . . . . . . . . . . . . .
SGE025RN. . . . . . . . . . . . . . . . . . . . .
SGE050RN. . . . . . . . . . . . . . . . . . . . .
SGE050TLL . . . . . . . . . . . . . . . . . . . .
SGE100RN. . . . . . . . . . . . . . . . . . . . .
SGE100TLL . . . . . . . . . . . . . . . . . . . .
SGE250TLL . . . . . . . . . . . . . . . . . . . .
SGE syringes . . . . . . . . . . . . . . . . . . .
SI-AOSU. . . . . . . . . . . . . . . . . . . . . . . .
SI-BAM21-LC . . . . . . . . . . . . . . . . . . . .
Item
Item
Page
TM33C05. . . . . . . . . . . . . . . . . . . . . . . 89
TM33C10. . . . . . . . . . . . . . . . . . . . . . . 89
TM33CCINS. . . . . . . . . . . . . . . . . . . . . 89
TM33CCNON. . . . . . . . . . . . . . . . . . . . 89
TM53CCINS. . . . . . . . . . . . . . . . . . . . . 89
TPP Electrode. . . . . . . . . . . . . . . . . . . . 69
Transbridge . . . . . . . . . . . . . . . . . . . . . 91
Transducer Amplifier . . . . . . . . . . . . . . 91
Transducers . . . . . . . . . . . . . . . . . . . . . 86
Transmission probes . . . . . . . . . . . . . 203
Trio. . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
TST33A001KT. . . . . . . . . . . . . . . . . . . 89
TST33A05KT. . . . . . . . . . . . . . . . . . . . 89
TST33A10KT. . . . . . . . . . . . . . . . . . . . 89
TST33A20KT. . . . . . . . . . . . . . . . . . . . 89
TST33AxKT . . . . . . . . . . . . . . . . . . . . . 89
TST33C05KT. . . . . . . . . . . . . . . . . . . . 89
TST53A10KT. . . . . . . . . . . . . . . . . . . . 89
TST150-6. . . . . . . . . . . . . . . . . . . . . . 116
TTL Control Module . . . . . . . . . . 167, 168
Tubing Cartridge. . . . . . . . . . . . . . . . . 167
Tungsten Halogen lamp. . . . . . . . . . . 149
tungsten light source . . . . . . . . . . . . . 199
Tungsten Microelectrodes. . . . . . . . . . . 88
Tungsten Wire . . . . . . . . . . . . . . . . . . 105
TW100-3. . . . . . . . . . . . . . . . . . 114, 131
TW100-4. . . . . . . . . . . . . . . . . . 114, 131
TW100-6. . . . . . . . . . . . . . . . . . 114, 131
TW100F-3. . . . . . . . . . . . . . . . . 114, 131
TW100F-4. . . . . . . . . . . . . . . . . 114, 131
TW100F-6. . . . . . . . . . . . . . . . . 114, 131
TW120-3. . . . . . . . . . . . . . . . . . 114, 131
Item
Page
TW120-4. . . . . . . . . . . . . . . . . . 114, 131
TW120-6. . . . . . . . . . . . . . . . . . 114, 131
TW120F-3. . . . . . . . . . . . . . . . . 114, 131
TW120F-4. . . . . . . . . . . . . . . . . 114, 131
TW120F-6. . . . . . . . . . . . . . . . . 114, 131
TW150-3. . . . . . . . . . . . . . . . . . 114, 131
TW150-4. . . . . . . . . . . . . . . . . . 114, 131
TW150-6. . . . . . . . . . . . . . . . . . 114, 131
TW150F-3. . . . . . . . . . . . . . . . . 114, 131
TW150F-4. . . . . . . . . . . . . . . . . 114, 131
TW150F-6. . . . . . . . . . . . . . . . . 114, 131
Twelve-Channel Pipetters. . . . . . . . . . 178
Two-barrel glass. . . . . . . . . . . . . . . . . 116
U
ultra-fine wire. . . . . . . . . . . . . . . . . . . 105
UltraMicroPump III. . . . . . . . . . . . . . . 182
UltraPath . . . . . . . . . . . . . . . . . . . . . . 194
UMP3. . . . . . . . . . . . . . . . . . . . . 182, 183
UMP3-2. . . . . . . . . . . . . . . . . . . . . . . 183
UMP3-3. . . . . . . . . . . . . . . . . . . . . . . 183
UMP3-4. . . . . . . . . . . . . . . . . . . . . . . 183
UMS. . . . . . . . . . . . . . . . . . . . . . . . . . 134
Uniaxial Tissue Tester. . . . . . . . . . . . . . 12
Universal Focus Mount. . . . . . . . . . . . 151
Universal Manipulator Stand . . . . . . . 134
Universal Pipette Tips. . . . . . . . . . . . . 181
UPUV. . . . . . . . . . . . . . . . . . . . . . . . . 195
UPVIS. . . . . . . . . . . . . . . . . . . . . . . . . 195
USBCAM33 . . . . . . . . . . . . . . . . . . . . 161
USBCAM50 . . . . . . . . . . . . . . . . . . . . 161
USBCAM133 . . . . . . . . . . . . . . . . . . . 161
Item
Page
USBCAM152 . . . . . . . . . . . . . . . . . . . 161
USBCAM202 . . . . . . . . . . . . . . . . . . . 161
USS1L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS1S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS2L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS2S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS3L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS3S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS4L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS4S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS5L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS5S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS6L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS6S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS7L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS7S . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS8L . . . . . . . . . . . . . . . . . . . . . . . . . 19
USS8S . . . . . . . . . . . . . . . . . . . . . . . . . 19
Ussing System. . . . . . . . . . . . . . . . . . . 18
Ussing Tubing Kit. . . . . . . . . . . . . . . . . 19
UV Safety Goggles. . . . . . . . . . . . . . . 198
V
Vannas Scissors. . . . . . . . . . . . . . . . . . 24
Vaporizer Pole Mount. . . . . . . . . . . . . . 35
Ventilator Connection Kit . . . . . . . . . . . 35
Ventilators . . . . . . . . . . . . . . . . . . . . . . 41
Vetbond . . . . . . . . . . . . . . . . . . . 100, 102
VFP . . . . . . . . . . . . . . . . . . 134, 136, 137
Vibration-Free Tables . . . . . . . . . . . . . 147
Vibration Isolation Platform . . . . . . . . 147
Video Camera. . . . . . . . . . . . . . . . . . . 162
Item
Page
Voltage Clamp . . . . . . . . . . . . . . . . . . . 17
Voltage Electrode . . . . . . . . . . . . . . . . . 17
Volumetric Flask. . . . . . . . . . . . . . . . . 113
von Frey Anesthesiometer . . . . . . . . . . 36
von Frey Probe. . . . . . . . . . . . . . . . . . . 36
V-Vette. . . . . . . . . . . . . . . . . . . . . . . . 202
V-VETTE. . . . . . . . . . . . . . . . . . . . . . . 202
W
W30 Professional-Grade Microscope. 153
W30S. . . . . . . . . . . . . . . . . . . . . 127, 153
W30ST. . . . . . . . . . . . . . . . . . . . 127, 153
Wall Mount Plate . . . . . . . . . . . . . . . . 149
Wall-Mount Plate. . . . . . . . . . . . . . . . 151
Waveguide Cleaning Kit. . . . . . . 195, 201
Window Discriminator. . . . . . . . . . . . . 81
Wire. . . . . . . . . . . . . . . . . . . . . . . . . . 105
Wire Cutters. . . . . . . . . . . . . . . . . . . . 103
WSA1001 . . . . . . . . . . . . . . . . . . . . . . 70
WSA2001 . . . . . . . . . . . . . . . . . . . . . . 70
X
xxxxD. . . . . . . . . . . . . . . . . . . . . . . . . .
Z
ZBEECAL . . . . . . . . . . . . . . . . . . . . . . . 67
Z-LITE. . . . . . . . . . . . . . . . . . . . . 149, 155
Z-LITE-186. . . . . . . . . . . . . . . . . 149, 155
Z-LITE-Z . . . . . . . . . . . . . . . . . . . . . . . 155
Z-LITE-Z186. . . . . . . . . . . . . . . . . . . . 155
Zoom Microscope. . . . . . . . . . . . 148, 150
Terms & Conditions
WPI values its customers and will make every effort to maintain the highest
professional and ethical standards. However, WPI regards as invalid any
special terms and conditions set forth by any other party, including purchasing,
quotations, warranties or other statements. WPI’s response to a quotation,
purchase order or inquiry shall not be construed as acceptance of any conditions
stated therein.
Credit Terms
PRODUCT INDEX
Net 30 days from date of invoice to customers with satisfactory credit references.
Standard Equipment & Ac­ces­so­ries
To insure against misunderstandings or delays, always include model num­ber,
name, and significant spec­i­fi­ca­tions of systems and ac­ces­so­ries when ordering.
Also specify any op­tions desired. WPI retains the right to change spec­i­fi­ca­tions
or discontinue products without prior notice and with­out re­spon­si­bil­i­ty for in­cor­
po­rat­ing such changes into equipment pre­vi­ous­ly sold. Please state the desired
operating line voltage and frequency.
Warranty
WPI strives to maintain the highest quality standards in all products. We
warranty these products against defects in workmanship or materials, and all
WPI products retain a warranty that is stated in the product’s manual. (Typically,
one year for parts and labor except for consumable items such as electrodes,
glass, etc., for which warranty varies from 30 days to six months.) However,
WPI hereby specifies that no use or application of any product may be suitable
or successful for a specific application and therefore waives all liability in that
regard. It is the sole responsibility of the buyer/user to apply the product in a
manner consistent with its intended use.
WPI products are not approved for human use unless specifically stated in
writing with accompanying FDA (or applicable) documents. WPI assumes no
liability or legal, moral, ethical, or fiduciary responsibility for a use of any
product in the WPI catalog in treating or studying humans.
Prices
WPI maintains competitive world wide pricing. Prices listed for standard items as
described are net and subject to change without notice. Prices (in U.S. dollars)
do not include shipping charges. Formal quo­ta­tions are valid for a period of
30 days unless otherwise specified. Pur­chas­es may be charged to your VISA,
MasterCard, or American Express account.
216
19
Taxes
We reserve the right to add any taxes required by local law or or­di­nance.
Institutions operating under tax-free conditions must state applicable license or
contract number and be prepared to furnish necessary exemption certificate.
Packaging and Shipping
Equipment is shipped in adequate commercial packing. All packages should be
checked thoroughly before being discarded. Shipping and handling expenses,
including adequate insurance coverage, are paid by the customer.
Claims and Returns
Inspect all shipments upon receipt. Missing cartons or obvious damage to
cartons should be noted on the delivery receipt before signing. Concealed
loss or damage should be reported at once to the carrier and an inspection
requested. All claims for shortage or damage must be made within ten (10)
days after receipt of ship­ment. Claims for lost shipments must be made within
thirty (30) days of receipt of invoice or other notification of shipment. Please
save damaged or pilfered cartons until claim is settled. In some instances,
photographic documentation may be required. Some items are time-sensitive;
WPI assumes no extended warranty or any liability for use beyond the date
specified on the container
Do not return any goods to us without obtaining prior approval and instructions
from our Returns Department. Goods returned unauthorized or by collect freight
may be refused. Goods accepted for restocking will be exchanged or credited to
your WPI account and are subject to a restock fee of up to 25%. Goods returned
which were ordered by customers in error are subject to a 25% restock fee.
Equip­ment which was built as a special order cannot be returned.
Repairs
Contact our Returns Department for assistance in the repair of ap­pa­ra­tus. Do
not return goods until instructions have been received. Returned items must be
securely packed to prevent further damage in transit. The Customer is re­spon­
si­ble for paying shipping expenses, including adequate insurance on all items
returned for repairs. Identification of the item(s) by model number, name, serial
number and proof of purchase (packing slip number or invoice number) as well
as complete description of the difficulties experienced should be written on the
RMA request form and a copy of the form must be included with the item.
Prices shown are in U.S. dollars. Actual charges willWorld
vary because
of import
duty, freight, and currency
fluctuations. To obtain an exact quotation, contact your WPI office.
Precision
Instruments
www.wpiinc.com
UK: Tel:
Tel: +44
01438-880025
• [email protected]
Germany:
Tel: 030-6188845
• [email protected]
China: Tel: 21 68885517
• [email protected]
UK:
(0)1462 424700
• [email protected]
Germany:
Tel: +49 (0)30-6188845
• [email protected]
US: Tel: 941-371-1003 • [email protected]
Ordering Information
Orders from North America should be directed to WPI’s main office:
World Precision Instruments, Inc.
175 Sarasota Center Boulevard
Sarasota, Florida 34240-9258
Telephone: 941-371-1003 (collect calls not accepted). Facsimile copies of
purchase orders, inquiries or other correspondence may be transmitted to
WPI’s Fax number: 941-377-5428. When sending written purchase orders
to confirm a telephone order, please clearly mark “CONFIRMING” on the
order to avoid it being shipped a second time. Our Sales Department’s e–
mail address is: [email protected]
Orders from Austria, Germany, Greece, Italy, Malta, Netherlands,
Russian States, Switzerland (DE) and Turkey should be directed to
WPI Germany: Zossener Strasse 55-58
D-10961 Berlin, Germany
Tel: +49 (0)30-6188845 Fax: +49 (0)30-6188670
E-mail: [email protected]
Orders from China, Hong Kong and Taiwan should be directed to
WPI China: WPI Shanghai Trading Co., Ltd., Rm 20a
No8 Dong Fang Rd., Lu Jia Zui Financial District
Shanghai PRC
Tel: + 86 688 85517
Email: [email protected]
North American customers may also order many products on-line through
WPI’s Web site — www.wpiinc.com. All customers may use the on-line
Quote Request Form and on-line Purchase Order Form.
Orders from Belgium, Denmark, Eire, Finland, Luxembourg,
Norway, Portugal, Spain, Sweden, Switzerland (FR) and the United
Kingdom should be directed to
WPI UK: 1 Hunting Gate
Hitchin, Hertfordshire SG4 0TJ England
Tel: +44 (0)1462 424700 Fax: +44 (0)1462 424701
E-mail: [email protected]
Orders from the countries listed below should be directed to the
appropriate WPI distributors. All other international orders should be
directed to WPI’s main office and factory in Sarasota, Florida.
To expedite shipment of orders outside North America (other than to
areas served by the international offices above), payment must be made
in advance via wire transfer, check payable in U.S. dollars, or charged to
a credit card. Proforma Invoices will gladly be furnished upon request.
Please specify proper electrical current for your order.
Customers in France may call +33 (0)970 44 9000 or e-mail
[email protected]
WPI Distributors
Argentina
India
México
Summit Research S.A
Rodrigo de Ibarrola 3140, 1D
C1419CGB Ciudad de Buenos Aires, Argentina
Telefono: (5411)-4566-8246
Fax: (5411) 4638-5049
E-mail: [email protected]
Labindia Instruments Pvt. Ltd.
Head Office: 201, Nand Chambers,
L B S Marg, Near Vandana Cinema,
Thane 400 602
Tel.: 022 – 2598 6062
Email: [email protected]
Website: www.labindia.com
Other Labindia offices: Chandigarh, Delhi, Gurgaon,
Lucknow, Guwahati, Baroda, Bhopal, Kolkata,
Pune, Hyderabad, Bangalore, Chennai, and
Thiruvananthapuram
Alta Tecnologia en Laboratorios S.A. de C.
Sucursales:
GUADALAJARA, JAL.
Av. Inglaterra #2588
Col. Arcos Vallarta
Guadalajara, Jalisco C.P. 44150
Telefono: 52 (33) 36 16 52 04 Y 36 16 24
69
Fax: 52 (33) 36 16 97 30
E-mail: [email protected]
Website: www.atlsadecv.com.mx
MEXICO D.F.
Comoporis #43, Col el Caracol
Coyoacan, Mexico D.F. C.P. 04739
Telefono: 52 555 606 24 69 Y 52 04
Fax: 52 555 665 79 68
E-mail: [email protected]
Website: www.atlsadecv.com.mx
Australia, New Zealand
Coherent Life Sciences Pty. Ltd.
116 Sir Donald Bradman Dr.
Hilton, SA 5033
Tel: (61) 8-8150-5200
Fax: (61) 8-8352-2020
E-mail: [email protected]
Brasil
Sellex (S.A.C.)
Rua Arandu, 205 / 1105
04562-030 - Sao Paulo - SP
Tel: (11) 5506-4646
Fax: (11) 5505-7433
E-mail: [email protected]
Website: www.sellex.com
Chile
Equilab Ltda.
DiazYCompañía Ltd.
El Quillay 627 sitio 96
Ciudad Empresarial Valle Grande
Santiago Chile
Fono: 56-2-4370217
Fax: 56-2-4650066
E-mail: [email protected]
Página Web: www.equilab.cl
Contact: Benilde Ardiles
Hungary, Poland
Experimetria Ltd.
15 Lapostelki Street
Balatonfüred, 8230 Hungary
Tel: +36-87-703-001
Fax: +36-87-703-002
Website: www.experimetria.com
E-mail: [email protected]
This Distributor also covers: Albania, Belarus,
Bosnia, Bulgaria, Croatia, Czech Republic,
Estonia, Herzegovina, Latvia, Lithuania,
Republic of Makedonija, Romania, Serbia and
Montenegro, Slovakia, Slovenia
Israel
NBT NewBiotechnology Ltd.
Uri Schechter, Managing Director
3 Mekor Haim St.
P.O. Box 8662
Jerusalem 91086
Tel: 972-2-6732001
Fax: 972-2-6731611
E-mail: [email protected]
Website: www.nbtltd.com
Japan
LMS Co., Ltd.
Tanaka Bldg.
3-6-7 Hongo
Bunkyo-Ku, Tokyo 113-0033
Tel: 03-5842-4171
Fax: 03-3818-7095
E-mail: [email protected]
Website: www.lms.co.jp
Physio-Tech Co., Ltd.
1-6-3 Iwamoto-cho, Chiyoda-ku
Tokyo 100-0032
Tel: 81-3-38642781
Fax: 81-3-38642787
E-mail: [email protected]
Korea
Scitech Korea Inc,
RM 801, 74 Gangbuk Electronics Industrial Complex,
Deongneung-ro 40-gil, Gangbuk-gu, Seoul 142-705,
Korea
Tel : +82 2 986 4413~5
Fax : +82 2 986 4429
Email : [email protected]
Singapore, Malaysia, Indonesia,
Thailand & Pakistan
Prime Bioscience Pte Ltd
Block 196 Pandan Loop #07-02
Pantech Business Hub
Singapore 128384
Tel: + 65 6364 0391
Fax: + 65 6269 0745
E-mail: [email protected]
Website: www.primebioscience.com
Muscle
Physiology
Loupes
See Page 31
2
Epithelial
Physiology
13
Microsdissecting
Microsurgery
Live Cell Imaging
21
See Page 156
Animal Surgery
& Support
34
Biosensing
53
Amplifiers,
Electrometers
71
Animal Temperature Controller
See Page 40
Stimulators
92
Benchtop Autoclave
Laboratory
Supplies
See Page 32
100
Cell Culture Platform
Glass, Holders,
Electrodes
See Page 11
Lab Glass
See Page 112
114
Microforges,
Pullers
129
Micromanipulators
132
Microscopes,
Cameras
148
Student
Microscope
Pumps,
Microinjection
See Page 3
165
Spectroscopy
192
Digital Stereotaxic
Index
See Page 45
209
Copyright © 2013,
World Precision nstruments, Inc.
All rights reserved.
Printed in USA.
Calcium Imaging
See Page 3
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